LOCUS       DAZOZX010000001        76592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZOZX010000001 DAZOZX010000000
VERSION     DAZOZX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.001"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..267
                     /locus_tag="ACHUPT_000001"
     CDS             <1..267
                     /locus_tag="ACHUPT_000001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817760.1"
                     /translation="KVVYGCMQGTGVIFRDTVTVSGCLLLRPLRLPAAWRHPVVRPDS
                     DGQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPVAP"
     gene            651..1178
                     /locus_tag="ACHUPT_000002"
     CDS             651..1178
                     /locus_tag="ACHUPT_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0817761.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEVREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            1236..1469
                     /locus_tag="ACHUPT_000003"
     CDS             1236..1469
                     /locus_tag="ACHUPT_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR0817762.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            1528..3486
                     /locus_tag="ACHUPT_000004"
     CDS             1528..3486
                     /locus_tag="ACHUPT_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0817763.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELAESVKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCIAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQVSLECKHYSLTSDAPSGKEGAAFLALMAEKVRL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            3541..3975
                     /gene="psiB"
                     /locus_tag="ACHUPT_000005"
     CDS             3541..3975
                     /gene="psiB"
                     /locus_tag="ACHUPT_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR0817764.1"
                     /translation="MKTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMVEGGQA"
     gene            3972..4691
                     /locus_tag="ACHUPT_000006"
     CDS             3972..4691
                     /locus_tag="ACHUPT_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR0817765.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            4688..5284
                     /locus_tag="ACHUPT_000007"
     CDS             4688..5284
                     /locus_tag="ACHUPT_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817766.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            5746..6246
                     /locus_tag="ACHUPT_000008"
     CDS             5746..6246
                     /locus_tag="ACHUPT_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFR0817767.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            6979..7413
                     /locus_tag="ACHUPT_000009"
     CDS             6979..7413
                     /locus_tag="ACHUPT_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFR0817768.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            7508..7774
                     /locus_tag="ACHUPT_000010"
     CDS             7508..7774
                     /locus_tag="ACHUPT_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817769.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            7951..8340
                     /locus_tag="ACHUPT_000011"
     CDS             7951..8340
                     /locus_tag="ACHUPT_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR0817770.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            8337..9215
                     /locus_tag="ACHUPT_000012"
     CDS             8337..9215
                     /locus_tag="ACHUPT_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0817771.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDTVSPEFMRALAERLPQHEDKLMTIAERLEQKGALEKALAIARQLQKMGMTPEQI
                     KQATGLSEAELKKIIH"
     gene            9274..9480
                     /locus_tag="ACHUPT_000013"
     CDS             9274..9480
                     /locus_tag="ACHUPT_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817772.1"
                     /translation="MTIAERLIQKGFDEGFKESFKEGFKEGALEVAREIACRLRDMGW
                     PPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(9511..9762)
                     /locus_tag="ACHUPT_000014"
     CDS             complement(9511..9762)
                     /locus_tag="ACHUPT_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0817773.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(9796..9992)
                     /locus_tag="ACHUPT_000015"
                     /pseudo
     CDS             complement(9796..9992)
                     /locus_tag="ACHUPT_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10341..11189
                     /locus_tag="ACHUPT_000016"
     CDS             10341..11189
                     /locus_tag="ACHUPT_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0817774.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEARNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKMNDIIARHYPG
                     ALPPRV"
     gene            complement(11275..11610)
                     /locus_tag="ACHUPT_000017"
     CDS             complement(11275..11610)
                     /locus_tag="ACHUPT_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR0817775.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKLSRRSSSKTAPKKTDSGS
                     KDGIDMNNN"
     gene            11843..12175
                     /gene="mobA"
                     /locus_tag="ACHUPT_000018"
     CDS             11843..12175
                     /gene="mobA"
                     /locus_tag="ACHUPT_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0817776.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            12187..14907
                     /locus_tag="ACHUPT_000019"
     CDS             12187..14907
                     /locus_tag="ACHUPT_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFR0817777.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AAELAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIDQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDKAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVVPGERFRG
                     HSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM"
     gene            complement(15128..17428)
                     /gene="trbC"
                     /locus_tag="ACHUPT_000020"
     CDS             complement(15128..17428)
                     /gene="trbC"
                     /locus_tag="ACHUPT_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR0817778.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKVILNTLLPLEILLPVAE
                     NNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(17409..18533)
                     /locus_tag="ACHUPT_000021"
     CDS             complement(17409..18533)
                     /locus_tag="ACHUPT_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFR0817779.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKY
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPKSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            complement(18530..19837)
                     /gene="trbA"
                     /locus_tag="ACHUPT_000022"
     CDS             complement(18530..19837)
                     /gene="trbA"
                     /locus_tag="ACHUPT_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFR0817780.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFALLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLAITANNAATITFGQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTEKQHPADKEKEQ"
     gene            20175..20321
                     /locus_tag="ACHUPT_000023"
     CDS             20175..20321
                     /locus_tag="ACHUPT_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817781.1"
                     /translation="MSGDLPSHQSIGTMDLCYSKTWPDKQVVLRLVLWPEEVSFQLTM
                     KELL"
     gene            20374..20469
                     /gene="dqlB"
                     /locus_tag="ACHUPT_000024"
     CDS             20374..20469
                     /gene="dqlB"
                     /locus_tag="ACHUPT_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR0817782.1"
                     /translation="MINLLIIVLRAVVAVANAVIALLELIRELVD"
     gene            21058..21153
                     /gene="dqlB"
                     /locus_tag="ACHUPT_000025"
     CDS             21058..21153
                     /gene="dqlB"
                     /locus_tag="ACHUPT_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR0817783.1"
                     /translation="MINLLIIVLRAVVAVANAVIALLELIRELVD"
     gene            complement(21216..21392)
                     /locus_tag="ACHUPT_000026"
     CDS             complement(21216..21392)
                     /locus_tag="ACHUPT_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817784.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(21543..22010)
                     /locus_tag="ACHUPT_000027"
     CDS             complement(21543..22010)
                     /locus_tag="ACHUPT_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0817785.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            22170..22793
                     /locus_tag="ACHUPT_000028"
     CDS             22170..22793
                     /locus_tag="ACHUPT_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0817786.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMSECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            complement(22991..23206)
                     /locus_tag="ACHUPT_000029"
     CDS             complement(22991..23206)
                     /locus_tag="ACHUPT_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817787.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(23210..23578)
                     /locus_tag="ACHUPT_000030"
     CDS             complement(23210..23578)
                     /locus_tag="ACHUPT_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817788.1"
                     /translation="MKDNNTTSTPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            complement(23878..24120)
                     /locus_tag="ACHUPT_000031"
     CDS             complement(23878..24120)
                     /locus_tag="ACHUPT_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817789.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            24450..24602
                     /locus_tag="ACHUPT_000032"
     CDS             24450..24602
                     /locus_tag="ACHUPT_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0817790.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVLVATL
                     AYEVKR"
     gene            complement(24740..26326)
                     /locus_tag="ACHUPT_000033"
     CDS             complement(24740..26326)
                     /locus_tag="ACHUPT_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0817791.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSDLAAKFIIFTAGIPKARRDEIKSSLSLISQLQKQRLR
                     ERRAELAHARAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQLNESDCHN"
     gene            complement(26600..27247)
                     /gene="excA"
                     /locus_tag="ACHUPT_000034"
     CDS             complement(26600..27247)
                     /gene="excA"
                     /locus_tag="ACHUPT_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFR0817792.1"
                     /translation="MSTTKSRYPSWWPYYYLVRNAYFIFGIPCLLFYAFFGTLIVHDS
                     ALSQTMDFIFAYGCWALIILPLLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(27335..29494)
                     /locus_tag="ACHUPT_000035"
     CDS             complement(27335..29494)
                     /locus_tag="ACHUPT_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFR0817793.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLNDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTNTSSTEYRTAEAAEPKSIIMKVWSGENFADKIATSLSGTGTSSD
                     QLNPLIKMKNIGDYTMGITESIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAKAF
                     LEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLLIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNMRRTPGVREEKLKNVNGDDKDGIKQ"
     gene            complement(29569..30138)
                     /gene="traX"
                     /locus_tag="ACHUPT_000036"
     CDS             complement(29569..30138)
                     /gene="traX"
                     /locus_tag="ACHUPT_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFR0817794.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            complement(30135..31340)
                     /gene="traW"
                     /locus_tag="ACHUPT_000037"
     CDS             complement(30135..31340)
                     /gene="traW"
                     /locus_tag="ACHUPT_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFR0817795.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            complement(31298..31918)
                     /locus_tag="ACHUPT_000038"
     CDS             complement(31298..31918)
                     /locus_tag="ACHUPT_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFR0817796.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            complement(31918..34962)
                     /locus_tag="ACHUPT_000039"
     CDS             complement(31918..34962)
                     /locus_tag="ACHUPT_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0817797.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKNHQTHVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            complement(35257..36075)
                     /locus_tag="ACHUPT_000040"
     CDS             complement(35257..36075)
                     /locus_tag="ACHUPT_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFR0817798.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            complement(35990..36241)
                     /locus_tag="ACHUPT_000041"
     CDS             complement(35990..36241)
                     /locus_tag="ACHUPT_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFR0817799.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            complement(36298..36696)
                     /locus_tag="ACHUPT_000042"
     CDS             complement(36298..36696)
                     /locus_tag="ACHUPT_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFR0817800.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            complement(36743..37273)
                     /gene="traQ"
                     /locus_tag="ACHUPT_000043"
     CDS             complement(36743..37273)
                     /gene="traQ"
                     /locus_tag="ACHUPT_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFR0817801.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAA
                     AGAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVF
                     NDRVIDATLASLGIAF"
     gene            complement(37270..37983)
                     /gene="traP"
                     /locus_tag="ACHUPT_000044"
     CDS             complement(37270..37983)
                     /gene="traP"
                     /locus_tag="ACHUPT_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFR0817802.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGSGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            complement(37980..39317)
                     /gene="traO"
                     /locus_tag="ACHUPT_000045"
     CDS             complement(37980..39317)
                     /gene="traO"
                     /locus_tag="ACHUPT_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFR0817803.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGAPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            complement(39321..40295)
                     /locus_tag="ACHUPT_000046"
     CDS             complement(39321..40295)
                     /locus_tag="ACHUPT_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFR0817804.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     QGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            complement(40306..41001)
                     /locus_tag="ACHUPT_000047"
     CDS             complement(40306..41001)
                     /locus_tag="ACHUPT_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFR0817805.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            complement(41013..41363)
                     /locus_tag="ACHUPT_000048"
     CDS             complement(41013..41363)
                     /locus_tag="ACHUPT_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0817806.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            complement(41380..45441)
                     /locus_tag="ACHUPT_000049"
     CDS             complement(41380..45441)
                     /locus_tag="ACHUPT_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR0817807.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSEDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTAETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKLRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEHPAAPSSAPEKAPVSAQPQQPDT
                     PVEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            complement(45505..45795)
                     /gene="icmT"
                     /locus_tag="ACHUPT_000050"
     CDS             complement(45505..45795)
                     /gene="icmT"
                     /locus_tag="ACHUPT_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HFR0817808.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            complement(45792..46940)
                     /gene="traJ"
                     /locus_tag="ACHUPT_000051"
     CDS             complement(45792..46940)
                     /gene="traJ"
                     /locus_tag="ACHUPT_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFR0817809.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            complement(46924..47760)
                     /locus_tag="ACHUPT_000052"
     CDS             complement(46924..47760)
                     /locus_tag="ACHUPT_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR0817810.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            complement(47757..48215)
                     /locus_tag="ACHUPT_000053"
     CDS             complement(47757..48215)
                     /locus_tag="ACHUPT_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFR0817811.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            complement(48319..49521)
                     /locus_tag="ACHUPT_000054"
     CDS             complement(48319..49521)
                     /locus_tag="ACHUPT_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFR0817812.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTFGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLVSNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            complement(49623..50444)
                     /locus_tag="ACHUPT_000055"
     CDS             complement(49623..50444)
                     /locus_tag="ACHUPT_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFR0817813.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PVRMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            complement(50657..51781)
                     /locus_tag="ACHUPT_000056"
     CDS             complement(50657..51781)
                     /locus_tag="ACHUPT_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0817814.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     MAERYMDEITTVDIATYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIASVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFRDKNQQLYVIFHLALETAMRQGEI
                     LTLRWEHLDLQHGVAHLPETKNGLPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDAKRKQTSKISPYFVPYPATVGCRNGLFVVTLHDFDLETRAETRELAISH
                     ASVLLLRTLAQAAQRGERVPTPGELPANIDERVMICPLTN"
     gene            51878..52075
                     /locus_tag="ACHUPT_000057"
     CDS             51878..52075
                     /locus_tag="ACHUPT_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon protein C'"
                     /protein_id="HFR0817815.1"
                     /translation="MNYSACKWYQSSVAMNHFIGGKSGGSIYYKPIQCPTGFIMTGTR
                     MYGIGDGVDEEHVDAYCCPFG"
     gene            52828..53106
                     /locus_tag="ACHUPT_000058"
     CDS             52828..53106
                     /locus_tag="ACHUPT_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817816.1"
                     /translation="MAMGSVVHLLFFLSEQCYLMPFSTYRIGTEASANPVCGERLVHL
                     TEVTAIWVLTGEHLTGEITAEELCLCTLPVEMVPVAVIAPTHQIFRAM"
     gene            complement(53278..54639)
                     /gene="pilV"
                     /locus_tag="ACHUPT_000059"
     CDS             complement(53278..54639)
                     /gene="pilV"
                     /locus_tag="ACHUPT_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR0817817.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITSGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            complement(54657..55283)
                     /locus_tag="ACHUPT_000060"
     CDS             complement(54657..55283)
                     /locus_tag="ACHUPT_000060"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0817818.1"
                     /translation="MLQMIPALLVAITVVIQCLAVQMSRYRLSRMNASPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLRCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            complement(55299..55784)
                     /locus_tag="ACHUPT_000061"
     CDS             complement(55299..55784)
                     /locus_tag="ACHUPT_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0817819.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            complement(55829..56365)
                     /locus_tag="ACHUPT_000062"
     CDS             complement(55829..56365)
                     /locus_tag="ACHUPT_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR0817820.1"
                     /translation="MSVITCSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            complement(56427..57521)
                     /locus_tag="ACHUPT_000063"
     CDS             complement(56427..57521)
                     /locus_tag="ACHUPT_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR0817821.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSELVIGYGLWLLAGLLLLAILIRMSFSLYKGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNSGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKSVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            complement(57523..59031)
                     /locus_tag="ACHUPT_000064"
     CDS             complement(57523..59031)
                     /locus_tag="ACHUPT_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR0817822.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIRVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            complement(59134..59592)
                     /gene="pilP"
                     /locus_tag="ACHUPT_000065"
     CDS             complement(59134..59592)
                     /gene="pilP"
                     /locus_tag="ACHUPT_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR0817823.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVQP
                     QITARIQLPGGRTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            complement(59582..60877)
                     /gene="pilO2"
                     /locus_tag="ACHUPT_000066"
     CDS             complement(59582..60877)
                     /gene="pilO2"
                     /locus_tag="ACHUPT_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR0817824.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLESGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            complement(60898..62517)
                     /locus_tag="ACHUPT_000067"
     CDS             complement(60898..62517)
                     /locus_tag="ACHUPT_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR0817825.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISENCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            complement(62549..62962)
                     /gene="pilM"
                     /locus_tag="ACHUPT_000068"
     CDS             complement(62549..62962)
                     /gene="pilM"
                     /locus_tag="ACHUPT_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR0817826.1"
                     /translation="MVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIRYMNAINDY
                     LYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGALLEQSGDSA
                     LLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            complement(62990..64060)
                     /locus_tag="ACHUPT_000069"
     CDS             complement(62990..64060)
                     /locus_tag="ACHUPT_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFR0817827.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            complement(64287..64622)
                     /locus_tag="ACHUPT_000070"
     CDS             complement(64287..64622)
                     /locus_tag="ACHUPT_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817828.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            complement(64739..64981)
                     /locus_tag="ACHUPT_000071"
     CDS             complement(64739..64981)
                     /locus_tag="ACHUPT_000071"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR0817829.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(65062..66762)
                     /locus_tag="ACHUPT_000072"
     CDS             complement(65062..66762)
                     /locus_tag="ACHUPT_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0817830.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVDVAVAFFLR
                     VKPSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQ
                     GVQNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRER
                     NEVEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAER
                     RREAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIA
                     VAAKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAV
                     ELTVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFR
                     LALLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTA
                     ALAYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV
                     "
     gene            complement(66789..67409)
                     /locus_tag="ACHUPT_000073"
     CDS             complement(66789..67409)
                     /locus_tag="ACHUPT_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0817831.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(67676..68338)
                     /locus_tag="ACHUPT_000074"
     CDS             complement(67676..68338)
                     /locus_tag="ACHUPT_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFR0817832.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            complement(68479..69120)
                     /locus_tag="ACHUPT_000075"
     CDS             complement(68479..69120)
                     /locus_tag="ACHUPT_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR0817833.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            69824..70696
                     /gene="repA"
                     /locus_tag="ACHUPT_000076"
     CDS             69824..70696
                     /gene="repA"
                     /locus_tag="ACHUPT_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0817834.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            71391..71549
                     /locus_tag="ACHUPT_000077"
     CDS             71391..71549
                     /locus_tag="ACHUPT_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817835.1"
                     /translation="MAENGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
     gene            71604..71873
                     /gene="yacA"
                     /locus_tag="ACHUPT_000078"
     CDS             71604..71873
                     /gene="yacA"
                     /locus_tag="ACHUPT_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR0817836.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVSDK"
     gene            71873..72151
                     /gene="yacB"
                     /locus_tag="ACHUPT_000079"
     CDS             71873..72151
                     /gene="yacB"
                     /locus_tag="ACHUPT_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFR0817837.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYVLRVLHTAQKWP"
     gene            complement(72347..72643)
                     /locus_tag="ACHUPT_000080"
     CDS             complement(72347..72643)
                     /locus_tag="ACHUPT_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0817838.1"
                     /translation="MIIHDIKREMPLKYGLYRVAKWFAWLAHTGIFCTFIIYIGFSII
                     TQHAGQELPETFKHGFALTFCSFATAALVSQWIGGGLHSKLEERIRMKWQNHAH"
     gene            72988..73575
                     /locus_tag="ACHUPT_000081"
     CDS             72988..73575
                     /locus_tag="ACHUPT_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFR0817839.1"
                     /translation="MLIKNPISYFHIGTFLFSGKKRHLLECWKAEVIMRDKFVFHRLI
                     RNGMQRQRGFLLWWRLANEMYISGNKKQRKCAIKIKNALMAKYGCDIGLGAQIGKGLV
                     LPHHSGVVIHGNVKIGENVIIRQNTTIGEKESDSRENYIVIGDNVDIGAHTCIIGLNV
                     KIGSNVKIGAMSFIMEEVPDNCTYVTRKESRVIMR"
     gene            complement(73857..74450)
                     /locus_tag="ACHUPT_000082"
     CDS             complement(73857..74450)
                     /locus_tag="ACHUPT_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR0817840.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAESPDTELSMDEWLCAVSCITSRRVYLQRTSVAGVPRYGL
                     DGHPDGQVSETEAQSAVQRLAIIEQRWLRMKERQTEQ"
     gene            74804..76150
                     /gene="yagA"
                     /locus_tag="ACHUPT_000083"
     CDS             74804..76150
                     /gene="yagA"
                     /locus_tag="ACHUPT_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFR0817841.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
BASE COUNT        17097 a        19252 c        21357 g        18886 t
ORIGIN      
        1 aaggtagtgt atggctgtat gcagggaaca ggagtgatat tccgtgacac tgtcaccgtt
       61 tctggctgtc ttttattgcg cccacttcgg ctcccggctg cctggcggca tccggtcgtc
      121 aggcccgact ccgacgggca gctgctggcg cacccgccag tctccgccga acccgctggc
      181 gcggtttcgg gctgccgcta cggtccgatg cgtgaaaacc cggcttgcag cgaccgttcc
      241 ggtcgcgccg cccctgtcgc gccgtgacct gcgctccctg cacacctgac ggtgtgtgag
      301 gaaactgcgc ctgatggcgc agcagtcctc cctcctgccg ccactcctgc ggcctgtttc
      361 ccgctgtgga ctttccgggg ggcagacgta accccctccc gttccatctg tccagggtga
      421 gatgcaccgg ccacaaaatt tttagccatg aacccggcaa aaaattttgc gtccgccccc
      481 ggacagctgc cccgtgaggc ggtaaacggg cccccatcgg aaaggtccac tgcggggaga
      541 aacccctgtg aagtggacgg caggaggagc tttcgctcct ctgaaggggc acatgaacgt
      601 cccgtcgccg gtatccggtt tatctcacat ctttcagtca ggaggttctt atgagtgcac
      661 gtggtatcaa caaggtcatc ctcgtcgggc gtctgggcaa tgatccggaa gtccgttaca
      721 tccccaacgg gggcgcagtg gcaaacctgc aggtggctac gtcagaaagc tggcgcgaca
      781 aacagacggg ggaggtgcgg gagcagacgg aatggcaccg cgtggtgctg ttcggcaagc
      841 tcgcggaagt ggcaggtgaa tatctgcgca agggtgcgca ggtctacatc gaaggtcagc
      901 ttcgcacccg tagctgggaa gataacggta tcacccgtta cgtcactgaa attcttgtta
      961 agaccacggg caccgtgcag atgctgggac gtgcaccaca gcagaacgct caggcgcaac
     1021 cgaagcctca gcagaatggg cagccacaga gtgctgacgc gacgaaaaaa ggtggcgcga
     1081 aaacgaaagg ccgtggacgt aaggccgcgc agccagagcc tcagccgcaa ccgccggagg
     1141 gggaggatta cgggttttca gacgacatcc cgttctgatc gggctgactg tgacaaccgc
     1201 cccgccctgt gcggggcatc accggagata tgaggatgag cgaatatttc agaatatttc
     1261 aggggctgcc tgacggctcc tttacccgcg aacaggctga agccgttgcc gcacagtacc
     1321 ggaatgtctt tatcgaggat gatcagggaa cgcattttcg cctggttgtc cgtcaggatg
     1381 gcacgttgat ctggcgctcc tggaattttg aggacggtgc cgggtactgg atgaaccagt
     1441 acatcaggga tttcgggatt cttaagtaag agaggtgccg gacgcgcgaa cgtccggcag
     1501 gacataagca attataaggg gatgattatg cctgtaacga agtgtgaacc agaaaccacc
     1561 cgcaaagcaa gccgtaaatc tgtaaaaacg caggaaactg cactgtctgc cctgctggcg
     1621 cagacggagg aagtgagcgt gccgctggat tcactgatta aatcaccgct gaatgtgcgc
     1681 acggtgccgt attctgcgga gtccgtcagt gaactggcgg agtccgttaa gggcgttggc
     1741 ctgttgcaga atctggtcgt gcataccctg cctggtgacc gttacggtgt cgccgcaggt
     1801 ggtcgccgac tggcagcact caacatgctg gcagagcgtg gcatcattcc ggctgactgg
     1861 cctgtacgcg tgaaggttat tccgcaggag ctggcgacgg ctgcatcaat gactgaaaac
     1921 ggtcatcgtc gggatatgca ccctgccgaa cagattgccg gatttcgtgc aatggcgcag
     1981 gaaggcaaaa cgcctgcaca aatcggtgat ttgctgggct attcgccccg ccacgttcag
     2041 cgaatgctga aactggcaga ccttgcgcct gtcatcctcg atgcgctggc agaagaccgc
     2101 atcaccacag agcactgtca ggcgctggcg ctggagaacg acaccgcgcg tcaggtgcag
     2161 gtgtttgaag ccgcctgcca gtcgggatgg ggcggtaaac cggaagtgca gaccattcgt
     2221 cgtctggtga ccgaaagtga agtggcggtg gcggggaaca gtaaattccg cttcgtgggg
     2281 gctgatgcct tctcgccaga cgaactgcgc accgatttgt tcagcgacga cgagggtggc
     2341 tatgtggact gtgttgcgct cgatgctgcc ctgctggaaa aactccaggc tgtcgctgaa
     2401 caccttcggg aagccgaagg ctgggaatgg tgcgccggac gtatggagcc tgttggttcc
     2461 tgccgtgagg atgccggaac ataccgcagt ctgccggagc cggaagcggt gctgacggag
     2521 gcagaagaag aacgcctgaa cgaactgatg gcacgttacg acgcgctgga aaaccagtgt
     2581 gaggaatccg acctgctgga agcagaaatg aagctgatgc gctgtatagc gaaggtcagg
     2641 gcgtggacgc cggaaatgcg tgccggaagc ggtgtggtgg tgtcctggcg ttacggtaat
     2701 gtgtgtgtcc agcgtggtgt gcagttgcgc agtgaggatg acgtggctga cgacgctgac
     2761 cgcacggaac aggtgcagga aaaagcgtca gtggaggaaa tcagcctgcc gttgctgacg
     2821 aaaatgtcct cagagcgcac gctggcagtc caggcagcac tgatgcagca accggacaaa
     2881 tctctggcac tgctggcatg gacgctctgc ctgaatgtgt ttggcagcgg agcgtacagt
     2941 aaaccagcac aagtcagcct ggaatgtaaa cattattcgc tgaccagcga tgcgccgtca
     3001 gggaaggaag gtgccgcatt cctggcgctg atggcagaaa aagtccgtct tgcagccctg
     3061 ctgccggagg gatggtcacg ggacatgacg acgttcctgt cactcagtca ggaggtgctg
     3121 ttatccctgc tcagcttctg taccgcgtgc agtcttaacg gtgtccagac ccgcgagtgt
     3181 ggtcacacgt cacgcagtcc gcttgactcg ctggaaagcg ccatcggctt tcacatgcgt
     3241 gactggtggc agccgacaaa agcaaacttc ttcggacacc tgaaaaagcc gcagattatc
     3301 gcagccctga atgaggcagg gctgtccggt gccgcacggg acgcggagaa gatgaagaaa
     3361 ggcgatgcgg ctgaacatgc agagttccat atgaaagaca accgctgggt gcctggctgg
     3421 atgtgttcac cacgtccaca gacggatgcc actgaacgcg ccgataacct ggctgatgcc
     3481 gcctgatgaa caaccacacc gccccgccgg agacggggcg gcagcaaggg agataccgtg
     3541 atgaaaactg aactgacact gaatgccctg cagtccatga atgcccagga atatgaagaa
     3601 atccgtgctg cgggaagcga tatgcgccgt aatctcactc acgaggtgat gcgtgaagtg
     3661 gacgcaccgg ctaactggat gatgaacgga gagtatggca gcgagttcgg gggctttttc
     3721 cccgtccagg ttcgtttcac gccagcccac gaacgtttcc acctggcatt atgttcgccg
     3781 ggagacgtct ctcagctctg gatgctggtt ctggtgaatg gtggtggaca gcctttcgcc
     3841 gtcgttcagg tgcaacatat cttcacgcct gtcgcgatca gtcacacgct ggcgcttgcc
     3901 gcgacactgg atgcgcaggg atacagtgtt aacgacatca tccatatcct gatggtagaa
     3961 ggaggtcagg catgagcgca cgttcacggg cactgatccc cctcagcgca gaacaacagg
     4021 ccgccatgca ggcggtggct gtcacagaac aacgtcgtcg tcagggacgc acactttcag
     4081 catggcctta tgccaccgct ttctttcgct gcctgaatgg cagtcgccgg atttcgctga
     4141 ccgatctccg cttttttgcc cctgcgctga cgaaggagga atttcatggc aaccgtctcc
     4201 tgtggctggc tgccgtggat aaactgattg aaagttttgg ggaagtctgt gttcttcccc
     4261 tgccatccga tgcggggcac cgtctgttcc cgtccgtgcc ttttcgtgaa ggtgagcgtc
     4321 gtcgtcagaa aaccacgctg acagagcaga aatacagccg ccagcgggaa cgtgaggcag
     4381 aacgacggga actggaatac cagacatgtt ttgctcaggc gcagattgac cttgcgtttc
     4441 atactcccgc cacggtcgga agctggttgt cccgctggtc tggtgttgtt gaggagcatg
     4501 atctggaaac gattttctgg gggtggtgcg ggcgttttcc atcactgtca tcatttgacc
     4561 ggtttttctg gcaggaggaa ccactctggc ggctgatttt tgaagccggt gaggccggtc
     4621 gtggtgcacc ggtacaggta cgtgcacttg agcagtggat gatcccgaac aagctggaga
     4681 acgtaatatg atgaaatcag acgaaaaata ccaggttccc gcgtggatgc gacctctgtt
     4741 gccgttgctc tgcaacaccg gggggaacga tccggaagaa ctgctgaatg atacagaaac
     4801 cactgccagt gcgaatgttg tccgttatgt actgatagtt gctgtgcggt cgcaggttga
     4861 tctgctgcag cttctgtaca ggaaagggct gttgcgcaca gagataccag gtggcttttc
     4921 accggaagaa gcgcaggcac tcctggataa tctggtgcgc agccatatca gcaaggcgct
     4981 gtcgggcgag cgaatggcag cccgtgacag aaatgccgat ctggcctgga ttggacagca
     5041 actggtcgat gccgcctggt ttgtccgtgc cacactggaa gcgcatggga tgagcgtcgg
     5101 aaatgagagt ccccctgctc cgccggagac aatgccggac atacagacac gggaactggt
     5161 gatgttgatc aagcgactgg catcatcgct gaaagcggtg aaaccggaca gttgtgtggt
     5221 gcgtgaagcg caggactggc tgcgcgacag aaaacttgtg gatatcacag atattctccg
     5281 gtgaaccagg catgtatatt ttacgcaggg acacgccgat catgatcgtt tcatatcagg
     5341 ggtggtctgg aggtcatgcg gtgtgctccc tgctctcgcc ggaataagga cgtcgccgcc
     5401 ggctccgctt ttacccggcc atgcggggca tggccttgtg ggttttcagt tatgtggccg
     5461 ccgtgtcgtg tgcgggctgt gccgtgcctc catcgtagcc gggcttctgc gggcgggatg
     5521 caaggtaaga tgaaccgctg cggtcacccg gtccctcctt cccgtctgcc gtgattttcc
     5581 ggtcgttcac ccgctcagat ttcgcggtct caccctccaa ctcccgccag ccgtgtgcgt
     5641 cgggctgcgg cttcccttgc gcccttgcat ccctgcaggc ccggcaaaga tggaggcacg
     5701 gcaccgcccg gtgtcgcaga acatgtgact atggaggatt cgggaatgtc tgttgttgca
     5761 cctgctgtat acgttggaac ctggcacaaa tacaactgtg gaagcatcgc tggccgctgg
     5821 tttgacctga caacgtttga tgatgaacgc gactttttcg ccgcctgccg tgcccttcac
     5881 caggatgaaa ccgacccgga actgatgttt caggattatg agggattccc ggggaatatg
     5941 gcctctgaat gccatatcaa ctgggcctgg gttgaaggct tccgccaggc gcgggatgaa
     6001 ggctgcgaag aggcttatcg tctctgggtg gatgataccg gtgagacgga ttttgacagc
     6061 ttccgcgatg cctggtgggg cgaggctgac agtgaggagg cttttgcggt tgagttcgtc
     6121 agtgataccg gcctgctggc tgacgtgccg gagacggtgg cgctctattt tgactatgag
     6181 gcgtatgcgc gggatttatt cctggactcc ttcaccttta ttgacggtca tgtgttccgt
     6241 cggtgattta ctccccgctc cggcgggggg atggcctgcc aggcccccgg aaaaccctgg
     6301 caggcttacg cctgcctgaa atacaggagg gccgcgcctg cggcgctacc cttcctgcct
     6361 gtcatagtgt gaggcatttt tctgttttgt cgtttctgtt gttctgactc ccgggggcgg
     6421 ggacaccctt cggctcccgg ctgcctggcg gcatccggtc gtcaggcccg actccgacag
     6481 gcagctgctg gcgcacccgc cagtctccgc cgaacccgct ggcgcggttt cgggctgccg
     6541 ctacggtccg atgtctgaaa aaccaggctt gcagcgaccg ttccggtcgc gccgcctctg
     6601 tcgcgccgtg acctgcgctc cctgcacacc tgacggtgtg taaggaaact gcgcctgatg
     6661 gcgcagttgt ctgcccttcc gccgcccccc ctgcggcctg tttccccgct gtggactttc
     6721 cgggggctga agcggccaca acacgtttca gccgtccagg ggagagatgt accagccaca
     6781 aaactttttg ccgcaagcgt caaaaacttt tgcgtctgcc cccggacaac tgccccgtgc
     6841 cgtggcagtc gctgccccat cggaaaggtc cactgcgggg agaaaccccg aagaagtgga
     6901 cggcagaagg ggctttcgcc cctccggggg agcaaccggt cggcccggcg acctgattca
     6961 gcagaaggag acgttttcat gacagtcagc agcacgattt ccgttttttg ccgcgacggg
     7021 gtgttccgta ccgtttactg ccacctgcac ggtgagccga cctggaacgg tcgcatcctg
     7081 catacccact atgccaccgg tcagcaggcc gaagccctgg ttgaacacgg tgatatccgt
     7141 tgcctcggtc cccgttgcga caaacccgcc gggcatacgc ttcagaaccc ggtggacggt
     7201 gtgacggctt attacggacg tgacagtggt ttccggatgg acagtgaggc gcgtgagtac
     7261 cgttctttca gggaggccat cgccacggaa agcactgaag aggtgcgctt ccattatgtg
     7321 ttcatcgacg gctactggaa ggtgatgtac cgcacgccgg aaggctggaa gatgaaagcg
     7381 ctcgcgctgg cactgcgtcg ctgtccggaa tgaaaaaaaa agcagggcga taaaccctgc
     7441 ctttctccgc tggcgcttcc ccgtacggaa gtccggcatc tcattcacta actatggaga
     7501 ttatgccatg agaccatcaa ttatcttcgc aaccgccgag tatgtaaagc gtctgcgaga
     7561 agagtgcctg cgggagaaca aacccctgca ccgccatacc cgcttcagac gccaggaact
     7621 ggcaccggat gagattaacc cggacgtcct ggcgatgggc ggtcatatcg ccagacgctg
     7681 cagtgagcag aagcgggtac gtatccccgc catgaaagtc agcgaatggg gccacctgct
     7741 ccgcgcgctt gaaattgaac gggtctgtca ctgattaacc cctgccgtct gcctgtgcag
     7801 gtggcaggat gtgttcaggt actttaccag gaggaaccgt gagcaagatg aaaaacatca
     7861 cgacgtctgt gttgcatggt gtctccacaa aaaacagaaa agggaaacag attctctttc
     7921 attcagggcc cgggagggct ggagataaca atgaagtctt gttttgatac tgacaaagct
     7981 tttcatatgt cttttgatga agtcactttc aacgctgaca gaattcgctc aaaactggaa
     8041 ctattatccg aagaactaat gccccgttgt ggtcttgttt atgagctata tcagcgacgt
     8101 ttgagtgctg ctattgataa ttttgtggcc acattgccga cagaacaaca tctactggtc
     8161 ctggaactgg cccgtgagga gtttgattac ctttccgcag aagagattgc tgaagaaata
     8221 cgccgtgact cagaacgcgg ctattgttgt catggctttg aacgcaattg ctgtcctctc
     8281 ggttgtgggg atttgggcga tgattgctgc gatgctgctg atttgatgga ggaaccgtga
     8341 gtaagaagaa aaacaccaca acgcccacgc cgcatgatgc cgcgttccgg tcgttcctgg
     8401 cgaatcccga cgtcgccaga gattttctgg aactgcatct tcctgcggag taccggcagt
     8461 tgtgcgacct gtccacgctg aagctggaac ccgccacctt tgtagagccg gacctgcatc
     8521 agtacgccag cgatatcctc tggagcgtga aaaccaccgg gggtgaagac ggctatgttt
     8581 atacgctcat cgagcaccag agcacagaaa atctgtacat gccttttcgc atgttacgct
     8641 acagtgtggc ggcgatgcag agacatctgg agcagcacaa aacgttgcca ctggtgattc
     8701 cggtgctgtt ctatcacggt gagcgcagcc cgtacccgta cagcatgaac tggctggact
     8761 gttttgagaa tccggcactt gcggctaaaa tatacacaaa gccgtttccg ctggttgata
     8821 tcactgtcgt tgatgacaat gaaatcatga accatcgccg gatggccgca ctgacgctgc
     8881 tgatgaagca tatccgccat cgtgacatga tggagctgct ggacaaactc ccgcaggtca
     8941 tggtggagat ttcagacgag caggtgcgtg ttctgattca ttacatcgtt aacgcagggg
     9001 acactgtatc accggaattt atgcgggcgc tggccgagcg cctgccgcag catgaggata
     9061 aactgatgac aatcgcagaa cgtcttgagc agaaaggtgc gcttgaaaag gccctggcca
     9121 ttgcgcgtca gcttcagaaa atggggatga cacctgagca gattaagcag gctaccggac
     9181 tgtccgaagc tgaactgaaa aaaatcattc actgatgaga gcatcacagc ccggcagaat
     9241 gccgggcttt tttgccagca ggggcagagc agtatgacta ttgcagaacg tcttatacag
     9301 aaaggtttcg acgaaggttt taaagaaagt tttaaagaag gctttaaaga aggggcgctt
     9361 gaagtggcgc gggaaatagc ctgccggctt cgggatatgg gctggccgcc ggaacggatt
     9421 caggaggcaa ccggactttc cggtgaagaa ctgaaaaaac tgtttcctga tgagcagtag
     9481 cctggcattc agccaggctg tgaattacgc tcacacatcc tctgccgttt cccccagttc
     9541 attttccagt cgcctgatga tatctgcctt gcgcatcccc tgcatgtgag gcagcaggtc
     9601 acggactgaa agtgcctggg tggtcctcca gacggtgaca tccacctgct ccgcattttc
     9661 ccaggcgtgc catgttctct gtgtcacgcc aaattctctg gccgcttttt cgcgtgacca
     9721 gagcatgctt tttcgccaga ttcgcagttc ccagccggtc ataaagtgcc ttttaaaaag
     9781 tgaaaaaact tcactttatt ttatcatcat aatcaccagg tcagcgcgat aaataagcgt
     9841 gaaaaagtga agaaatttca ctttttttag ttgtagatgt cgtgctgttt tctctgtttt
     9901 cacctgtgcg ggagtgggtg tcagtgacca ccacatgcca ttgttgtgcc gtcgtttttc
     9961 ttcagtcgcg ccggatgcag cgctccctgc acaccttacg gtgtgtaagg ccactgcgcc
    10021 tgatggcgca gctgtctctg cctgtctgcc gccgcactgc ggcctgttct ccgcagacgg
    10081 actttccggg ggtacataca tgaccacatc ccgtttcagt cgtccagggg agagatgtac
    10141 cagccacaaa actttttgcc gcaggcgtca aaaacttttg cgtctgcccc cggacaactg
    10201 ccccgtgacg tggtgagcgt cccccgtcgg aaaggtccac tgcggggaaa tccccgaaga
    10261 cccggacggc agaaggagct tcgctcctcc gggaaatgac ctggctgacc cggcagtcgt
    10321 gacgatgagg agacaatgtg atgaatcaga ctttacccac tgctgacctg aatactgccg
    10381 gcacgacaga tgttattccg tctgtggcta tcgaccgcat catcgcgcag cgtaacgaag
    10441 gtattgcact gttcatgcag gcgatggaat gcctggcgac agcgcgcaag attctgctcg
    10501 atgcgtcagg tgatattttt ctttacgggt ttgaagactg cgtgactgac tccgttcgat
    10561 gcatggataa accggaagaa gcaaaaaaga atatcacccg tcttgccgac cggaaaatct
    10621 gggatcgcct gatgacggat actggcatgt acaccttcat gagttcatgc cagcgtgatg
    10681 agtggaacag ccagctgatg agcgacacct gtcctgaaat caccctggac aatgtactgg
    10741 caacattccg ccatctgaat gccagcaaga tgcagacatt tgaacaggga ctgattgatg
    10801 tctaccggaa attgtcatgg gattacagaa ccaataatcc ctgccgtctg ggtaagaaaa
    10861 tcattattga aaacctgctg taccgctgga gtaacggacg tgtgacgctg gactgcagcg
    10921 gacgggaggc actggatgac ctggtacgtc cgttttatct gctggaggcg cgcaacgttc
    10981 ctgacttcag gaacagcatc ggggcgcagt atggtgaatt tctcgggaac ggcgacaatg
    11041 tcggtaagct attagaaggg gaatatttta cggtgcgtgg ctaccagaaa gggaccgtac
    11101 acattgtctt taagcgtcct gaccttgttg aaaaaatgaa tgatattatt gcacggcatt
    11161 atcctggtgc attgccgcca cgagtctgat taaacagaaa agccctggta tttatgccgg
    11221 ggcttttttt tctgtaatca tcataatctc agaacacgat gtgacattgt cacatcaatt
    11281 gttgttcata tcgatgccat ctttactgcc tgaatctgtc tttttaggtg ctgttttaga
    11341 ggaagaacgg cgggatagtt ttttcgattc tgcttcccga ataatgtcac gaagtgcctt
    11401 ctctcctact tcataccctg catttttgag cgtgtcacga acatctgcca gtgtataacc
    11461 ctttgtcctt accaggacaa taatgtcatc acgaatggca tcaagaaaat cacgcaatgt
    11521 tttccgttga gcggtgaggt ctggcaattc cgataaagca gcttttgcca gctggatgtc
    11581 atcatctgag taaaattttt tagacgccat tggcactttc tctccgttta atgttgaagc
    11641 ctgattttat catcaaaaac tgactcctgg cagggctgcg ccctgcctct gccgtctgca
    11701 taagactatg atgcacaaaa ataacaggct ataatggact gaaaaacggg acaggatgtg
    11761 taatcgtata accgcgcacg cacggctata cgattaccac ctggtcaggg cttagccccg
    11821 acaccccggt aaggagcaga ccatgagtga gaagaaaacc cgaagtggca gtgagaaacg
    11881 gcaaaagaat gtcctcattg ctgttcgttt ttcaccagaa gaggctgaga ttgttaaaga
    11941 aaaggcagaa aaaaacggac tgacagtttc aactctgata agaaaaactg tgctgggaaa
    12001 acagataaat gcccgcattg atgaagattt tttgaaagag ctgatgcgtc ttggtcgcct
    12061 gcagaagcat ctgtttgtgg aaggaaaacg cacgggagat aaagagtatg cagaggttct
    12121 cgttgctatt acagaacttg ctaatactct gcgaagagat ttaatgggac gctgagataa
    12181 tcgctgatga atgccatcat tccgaaaaaa agacgtgacg gaaagtcttc ttttgaagac
    12241 ctgattgcgt acacctcagt gcgggatgat gtgccggaag atgaactcag gcctgacacg
    12301 gaaatgaaag ctgagattcc gcaccggaac cgcttcagac gtcttgttga ttatgcaaca
    12361 cgtctgcgta atgaaaaatt tgtttccctg attgatgtga tgaaagacgg cagtcagtgg
    12421 gtgaatttct atggtgtgac ctgctttcat aactgtttgt ctcttgaatc tgcggctgaa
    12481 gaaatgcaga aagcagcaga gcttgctcat ttttccaaag atgatacaga ccctgttttt
    12541 cactatattc tctcctggcc tgcgcacgaa agtccgcgtt ctgagcagct ttttgactgt
    12601 gtgcgtcaca ccctgaaatc ccttgagctg tcaaaacatc agtatgtggc agccgttcat
    12661 acagacacgg ataacctgca tgtacatgtt gcagtgaatc gtgtccaccc tgaaaccggc
    12721 tatatcaatt gccttccgtg gagccaggaa aaactcagtc gtgcatgccg tgaacttgaa
    12781 cttaagcacg gattcgcgcc ggataatggt tgctttgtac atgcaccggg caatcgtatt
    12841 gtgcgcaaaa cagcgctggt acgtgagcgc cgtaatgcct ggcgacgcgg gaaaaagcag
    12901 acgttcaggg aatatattga tcagatgtcg attgccgggc tgcgtgaaga gccagcacag
    12961 gactggttat ctttgcataa acggctggcc agtgatggtc tgtatatcac gatgcaggaa
    13021 ggggaactgg tggtgaagga tggctgggac aaagcgcggg aaggtattgc actcagttcg
    13081 ttcggaccgt catggacagc tgaaaaactg gggcggaaac tgggggaata tcagcctgta
    13141 ccgacagata ttttcagtca ggtggggaca cccggtcgtt atgatccgga agccataaac
    13201 gttgatatcc ggccggaaaa agtggcagaa acggagagtc tgaaacagta cgcctgccgt
    13261 cattttgccg aacgcttacc ggcaatggcg cgcaatggtg aactgaaaag ctgcctggct
    13321 gtacaccgta ctctggcaga ggcgggatta tggatgggga ttcaacacgg gcatctggta
    13381 ctgcatgatg gatttgataa acaacagaca ccagtacgtg cagacagtgt gtggccgtta
    13441 atgacgctgg attatatgca ggatctggat ggtggatggc agcctgtacc gaaggatatt
    13501 tttactcagg tcgttcccgg agaacgattc cggggacaca gtattggctc tcagcctgtc
    13561 agtgattacg aatggtatcg tatgcggatg ggcacaggtc cgcagggggc tataaagcgt
    13621 gaactgtttt ctgataagga aagtctgtgg ggatacggcc ttattcagtg caggagcctg
    13681 attgaagaga tgatagctga cggggatttc agctggcagg catgtcatga gatgtttgca
    13741 cgtaaagggc ttatgctgca gaaacagcat catggtcttg tgattgtgga tgcgtttaat
    13801 catgagctga caccggtgaa ggccagcagt atccatcctg atttgacgtt atcccgtgcc
    13861 gagcctcagg ccgggccatt tgaaattgcg gctgcagata ttttcgaacg ggtgaagcct
    13921 gaatgtcgtt acaatccgga acttgctgcc agtgatgaag tggagcccgg cttccggcgc
    13981 gatcctgaga ttcgtcgtga gcgccgtgaa gcccgtgcag ctgcgcggga agatttgcgt
    14041 gcgcgttatc ttgcatggaa ggagcactgg cgtaaacccg acttacgtta cggagaacgg
    14101 ttacgggaaa ttcatgctgc ctgccgtcgg cgcaaggcgt atatccgtgt gcagttccgg
    14161 gatccacaat tgcgtaagct gcattaccat attgcggaag tgcagcgtat gcaggcgctg
    14221 atcaggctga aggagagtgt gaaggaagag cggttgtcac tgattgcgga gggtaagtgg
    14281 tatcctttgt cctaccggca gtgggtggaa caacaggcgg ctcagggaga cagggctgca
    14341 gtatcgcagt tacgtggctg ggattatcgt gatcgtcgct cgcgtaataa ggataaaaga
    14401 cgcacaacta atgttgatcg ttgtgtcgta ctctgcgaac ccggaggaac gccacttttt
    14461 aataatgttg caaaacttga agctcgtctt cagaaaaatg gaagtgttca tttcagggat
    14521 acgcggacag gaaaaaatgt ctgtacggat tacggagatc gggttgtgtt ctaccatcac
    14581 acggaccgaa atgaactggc agagaagctt aatttaattg cgccagttct gttcagccgc
    14641 aacggaaagc ttggttttga gccggaaggt tcataccagc agtttaatga tgtgtttgca
    14701 gaaatggtgg cctggcacaa tgcagcggga ataaccggaa acggacactt caccattact
    14761 cgtccggacg tggatttgca ccgtcaacgc agtgagcagt attaccggga atatatcaga
    14821 cagcagaccc gtttatcgga gagccatgat gataattata cgttacggca ggagaaaaca
    14881 tgggagccac catcacctgg aatgtaagcc cggataagtg atggtgcaaa gattcagccc
    14941 aaaaacggca ggaatcttcg tcaataaggg cgggagtagt cactgaattg agtttatacc
    15001 cggttaactc tgaattaagg gccgtgtctg cggcccttat ccagtgcgcc gtaaaacccc
    15061 ggacttagtg aacagggttt tacagtctga ttgacagata tggtcaccag aaaatatttg
    15121 tattccttca gtatttttcg tcagttgttg gttgtgctcc acatgatttc tggggatgat
    15181 cattatttat tgtctgcgat ttattatttt cagcgaccgg taacagtatc tccaggggga
    15241 gcagggtatt cagaatcacc ttcactgaag gtatatcttc aggaccggag tgttcctctt
    15301 ccggacgatt cagtgtgata taacataccc ggcgttcttc cctggttgtg ttattgatag
    15361 tgtgcacagc ctttttccac agttcgtttg cccgggattc gatatctgta tcatattgcg
    15421 gaagcagatc cagtgatgtc tgcgctgttg ccaactggcg ctggaatgta tcaatgatac
    15481 ggtaaggctc tgtacggttt ttacgacgtt tacgggatgg ttttgctgtc agtacaccga
    15541 tgatgctgct gacatgctcc ggtgtgggga gcctgcgctg cactgtcctg gggacgagac
    15601 ggcggagacg ctccagccgg ggggcttcaa cggaaatata gcggttgatg accaccggat
    15661 cactgtcgca acttttctcg ctgtccggaa tatacacaga ggctgacggc accacatccc
    15721 cctggaaaac ggtaaagaac tcaccggcat tcagtctgat caaatcctgc acgtcgattt
    15781 tgctttccat ctggatggtg atctggttac cgtccaccca ggactcaccc atcacaccat
    15841 cgtggcgctg caggctgccc attctggccc gtgcctcttc accggcggca tttttcaccg
    15901 ttcttgccgt tttgtcgtca ctgacaatgc gtccggcgaa tttggtgccg gcgttctgca
    15961 tcagtgtggc ggtgttggtg gcggaggtct gcccttcaat tcgctcctga tcctgcgccg
    16021 tcattatcag ggaaaactcc agtgaacgta cctgggtggc ctccacagca atgcggtctg
    16081 tgtagtaggc accgacttcg tcaaggatac agatataggg gaagctgcct ttgtattttt
    16141 tgacttccag ggtctgtgca tctgtgcctt ccagccggta gcccaggtca cgggcgagga
    16201 gcatgctctg ctgggtgacg aacatacgac cgagtgccga cgtggtatga gccgatgtgt
    16261 ccagtgcagg gatcatcaca atcagaatgc ggtcgctgtg aatactgtcg cgaatatcga
    16321 tgtcgccggc atctgccgca aagacgtccc cgaaggtttc ggcaaaggtt gtgaacgttt
    16381 ccgagaactg accggagaga taagcatgct gtttacgggg ctcctcagtc caggctgacg
    16441 gcgttctcac cagtgacata tcgaatcccg ggacatcctg caggtagttt cgcagtggtg
    16501 cgatagcttc ttccggccac tggtcatcaa ggccacggca atagagtttt gccatccctt
    16561 ccagcgtcat atgttcccgt aacatctgca gggacatggt tttaccttcc cggacgcacc
    16621 agaattttgt gccaaagacc agggccttgt tcatggctat cgcccgtgac tgccattcac
    16681 cgccctgaac gttctggggc agcatggact gcatggtttc ggcggtgaag gcttcggtgc
    16741 tgaatgcgaa cgggttccag gtgtttgact gtgggcggga tttttcgccg ctctgaatga
    16801 tttcactgcg ggattttccg ccgttcatga agttgatgac ctcgatatca tcctccctgc
    16861 caaaacgtcg ggagagatac cagattgtcc tcgtggtatc gttctgtgcc tttccgtcaa
    16921 caaaggtaaa gcctcttccc cagcagagcg ggttgagcag ccaggcaaag gtggtttctg
    16981 ttttaccgcc accggtggtg gcaaaaaaca tgacatgccg ggtgaggtca tccatgctca
    17041 gccagagctc acgaccgata tcattgatac ggcggtaacc gacataaaaa atcccccgtg
    17101 cgggggatgt ttcatctgct tcatactgaa acagggtggg ccagaagctg aagaggcttc
    17161 gcctgacctt tctgtcctgg gaggggtcat caaggctgag cgtcatgggc atacgcaggg
    17221 ggcatcgcca gcgttgcagc gtgaatatca cggaggtgac cagggtgaac agcagacaga
    17281 caggaagggc cagtggccat atcagggccg taatgatact gccggccaga cacagaccaa
    17341 tgatgttcag gttatgcagt gcattccaca gcgggtttcc ccaggccgtg cggtgtatga
    17401 ggtcaggatt aacgtggtgc tggctcattg tgatactcct cgctatctgt tgctttcagg
    17461 aagaggcgaa cggaagtggc gtctttcagg atgccggacg gcaggtgcca gccggtatct
    17521 gataacaccc agggggttcc gccaaaaccg aactggcgga aagcctggtt attgacatcg
    17581 atggcagcac gtgcggcggt cagacactgt ccatcctgtg cggccggtgc atcgccggtg
    17641 ggggccatac cactgtcagc ggaatacagc tgatgccatc cggtcgcccg tttttgtgag
    17701 tccggttcac acagcagcgg agcagtccgc tttgtactgg actccccgcc aatcacggag
    17761 accggataaa tcaccacgct gaattcccgg gccagttgct taagcgcggg ttccagcaga
    17821 cggcagttga tgcatgcggg gtcaaggaag gcgtacagcg tgcgttcatg accagtggac
    17881 aggttaatgg tgaacatccc ccggtctgcg ttgcgtttaa gtactgtggc cagattacgg
    17941 cgggcctcag cggcctgaac gtgttgttct tccgtcactg ccgtcggggc gggggcagaa
    18001 gaaggttcct gctgtgtggg agccgggcgg gccgggatgc tgttaacatg atgttccgta
    18061 tgtgggcgtg gtgtctctgt gtcggttact ggttcagctc tccattcctg agatttaggg
    18121 taagccagcg tggaaggggg ctctgccttc atccagtggt aacctgcaac tgtcgccggc
    18181 agagcaattc cggcgataaa gccacacatc agtgtccgcc agaagccata tttccgggag
    18241 ggatgttttc cttttccggc atattcggca tccaggcagg catcaatcgc tgagagcatt
    18301 ctgccgatgt gatgtccggg acgttctgca tcaacggtat caacaagcac cgtttcggtt
    18361 atttcgcgaa aaggggaact gaaggacagg tgcatcagcc tgttattacg gttaatcagt
    18421 gcgcccagtt ctgttaccgg caggatggca atcacctccc gattaccgtc agggtacagg
    18481 gcggtcaggg acaggtgttt attattcagt gttgcagtgt agtggatggt cattgttctt
    18541 tttccttgtc ggcgggatgc tgtttttcgg tggtttcggt ggtttcggtg gtttcggtgg
    18601 tttcggtggt ttcggtggtt tcaccagtgt cagtgtcttc gtcgttatca tggcggcggg
    18661 gtatcacgtc atccgtgagt gaccagcggg tcctgatctg acgtttacga tcgcgatctg
    18721 gctgaggttc gtcccatatc aggcccagcc ccagcatgtc ccgtcgaagc ccttcaatgg
    18781 cctcttccac acagggttcc gggcagggga ggccgagacg gctggcttca ttttctgccc
    18841 gggcaacggc caccacgccg gccccttcga tgaagccttt tgtggtgttt gcccggtgaa
    18901 gggcatagaa gagcgtccgg tcaacgccct tgagccacag ccagttcgga ggcgtcaggc
    18961 gcaaatcatg tgcgtaaagc cagaccagac cgctgcgaac gtaacggtgc tgcctgagcc
    19021 actgttgtgc gccttctgtt ttgatgaccc ggataaaggc gtttctggcc agtgaataca
    19081 ccggcgtgct gaaacgcccc tggtcccgtt tacttttgag cctgcaggag agattgaggt
    19141 tgttcatcag ggctaccgct gctttccggt caccgaggaa gaactgcagg ccaaagatgg
    19201 caaagagcgc tttttcatgt ggggccatgt ctttccagct ggtcagtggt tgccccagtt
    19261 gcgccatgaa gcactggcgt gtggacgcaa catcgagctg catgttgcgg ataaggttgt
    19321 tctgctccac aaagacttcg ggggttagtg cgacacggcg ctcgggacgt ttttgtccgt
    19381 ggaagagtct gttgttgtct ccatcagcca gaacgggggc gatcgccggt gacagagctg
    19441 aaaaaatatg gggcagtgtg tggatattca gtggcctgcg ggtaaaacgg ggctgggccg
    19501 gatggtgaaa ccacatccac agaaagccga atgtcatggg aagcagaatc aaccagagga
    19561 tccccactgt ccgggacata acatccagcc actggccgaa agtgatggtt gccgcgttgt
    19621 tagcagtgat ggccagcagg ttgtaacggg gggcggcaaa atggtgaaaa ggcccccagt
    19681 cagtcatccc ccacattgtg tgcaggatca ggcaggtggc aaagacgatg tcgggtaaaa
    19741 agagccagat gagaagcgca aagagcagac atatggtgcc aacgatatac catatgttca
    19801 tcgggtcttc tgttgtgggg gagtgttggt aagacacgag gattcctcca tcgataaata
    19861 gataaatttc gggatgaggc tgagtggaat taatgcgaac aatcgccggt aatgaccggg
    19921 cagggctgaa ttcagcaggg cattttcaca ggaacagcag aaggtggtga gagacaggca
    19981 caaaaaagcc gactggtgag gccggctttt ttgcgatccc agggagagtg ggtggctctg
    20041 gcccggggga agtctctttt caactaacgt taccagaata tcgttatatt cacctgatcg
    20101 caatctgttc gttacagtct attgttccgg gagtagcgat atcgcctgaa acaagtataa
    20161 tcagcaagtt agccttgtct ggagacttac cttcgcatca atccatcggg actatggatc
    20221 tgtgctattc aaagacctgg ccggacaaac aggtggtgct gcgtctggtg ctctggccgg
    20281 aggaagtctc ttttcagcta acaatgaaag agctgttgtg acaggcaatg taagagtaaa
    20341 caacagtgat ttcctccatt gatggagaaa cggatgatta acctgttaat catcgttctc
    20401 agggcggttg ttgctgtggc taacgcagtg atagctctcc tggaactgat ccgggaactg
    20461 gttgactgat aatcccggaa acgtacttaa gggcagaggg tcaccgccct ctgcccttta
    20521 ataatctacg gacagattac tttctgtact tccagagcct gtttctgtga gtcaaacatg
    20581 agctggtttt taccgactac acccaaggaa atatattcga tatctcccat ttgccgtatt
    20641 ttcgtaaaat tcaacaggaa atattcaacg ggagtgagag aactgctgtc cggggaggag
    20701 gtacagacgc aaaaaaaccg actggtgagg ccggtttttt tgcgattccg gatcgaagtt
    20761 ggtagccctg accggaagat aactgcctat aactgactat gaaataatac ggctgaaata
    20821 atatttctgc aagcacagta taagtatata ctcggtgtgt cagctttgag gcacgttctt
    20881 tccggcatat cgttcaccgg gatgaacagg ttgcaagtcc tggccaaaac atcaggtggt
    20941 gctggttttg gtgctctggc cggagaagaa ctgtctataa ctgacactat agaaaacgtg
    21001 gcgacaggca atgtaagggc aaaccacggt gttctttctc cattgatgga gaagcggatg
    21061 attaacctgt taatcatcgt tctcagggcg gttgttgctg tggctaacgc agtgatagct
    21121 ctcctggaac tgatccggga actggttgac tgataatccc ggaaacgtac ttaagggcag
    21181 agggtcaccg ccttctgccc tttaacaaaa tatgctcagg ttaaaaatcg aaaaccttcc
    21241 tgcacttcct gtgcctgttc ccgtgagtca aacatgagcc ggcttttacc cggaactccc
    21301 caggaaatat attctacatc cacccaccat tttccgtatt cttcatacgg tccggatatc
    21361 actttagtga caaatgcatc aaccaggttc atttttacac ctttctgtta ttctgcttat
    21421 tacgaccagc agacagcaaa tattaaaaat aacgcggcgg taaaaatggc aatttttttc
    21481 catgctgtat ttttgccatt tattttcttt gaaatggttt ttttcttcat atcagcaaat
    21541 aattatttct tattctgttt tctccaggtc catggtggta ccggattttt atctgcccat
    21601 aatccccgct tcagggcgcg ggcatcacgc tggagcggcg ggagagtata gtcattattg
    21661 tagtccgggt acacccaggc agcgccgtta cggacctgcc agcgatttgc ttctgtgccg
    21721 tcagcggtat agacccggcc gagaatacgg ccatacctgt ctgtctgcgc gtattgcacg
    21781 gttacgggct ttccggccac caggtccttc agctgagtgg ctgaccagcg tccaaagggc
    21841 tgtttctttt ccggtgcatc aatattagcc agacggaccc tgacaggctt tttgtcctgt
    21901 aatatttcca gcgtatcacc atcaagaacg cggatgacgt gcccctgaat ctgtgcagca
    21961 cagagagaag cggaaaaaat aatggtgagt gaaaagatat attttatcat ttcatcgtct
    22021 cctttttatt tatgaaaaca ggtgcaatta atattgcgaa ataataacgt tcaggtgtgt
    22081 tttttttggc agtgtgatac ttcacgcagg gcatgaattc cagtacgctg atatccgtga
    22141 tgatacggca acagatatcc ggtgacggta tgcaggaaca tattgattat cagattgaga
    22201 aatatcagtt cagggccagg aatgagtcac cgcgactgat gcgccagtgg gaagatgtca
    22261 tgagtgaatg ccgtgatacc ggagcaggag ccagggagcg gctgcggatt gccttactta
    22321 atgttgatta tgtgaccagt tttgaactgc cgtttcgtct gctgttaacc cggacaccgc
    22381 agatgattga cagcgtgcgg cgggaatggc agctcagcca gaaaaaagtt gtattcaatg
    22441 agcggcagcg gggatgtgta tacagtctgc agaatgactt atccggcgtg ccggatagct
    22501 tcagttacag tcttgcgacg cgaatacggc gggcggacag ctatggcatg acgacgcgtt
    22561 gtttcactga tatagccggg caggttaaag ccccgacagc acggctgaaa ctggcgctgg
    22621 aaagcgggtt aattgtcacc gcgctggatg gtctgttctg gcttggtatt caacgtattg
    22681 ccgcagatgt gcagcggctg cgtcagtccg gaatgacaat catcacccgg gatgttgagg
    22741 tctcagactc gctgaccgga accacacggg ttgtttccgg gtacagcctt tgatttatgc
    22801 aacaaagtat cgcagcgtcg aatatgagct atggccagat ttttgttaat cgtggttgtt
    22861 gcaacatgta aggcgaatga ccacactatt ctggcacgct cgtaatcatg ctgaaattct
    22921 gcttcctgtc ctgccgtgat gagttccgac caggacatac ggcgaagtgt atgtctgtca
    22981 gtaatatccg tcatgagctc tcagcctcct ccttcgtatt tccgtcagaa acacaaacgc
    23041 cccgcagaga aaattcgcca acattctcct ttttaatggc gcgcaatgca tcgcggcagg
    23101 cccgttcggt atgaaaggcc tggctgtgca ccgccagtgt gggctgacgg gcatcaccgg
    23161 aagagacatt aaagggcgat gaaagccaga aaatcagtag ccacatgcgt cacacctcgt
    23221 cctttttatg cgggtatctg gacagaatat tgagcgcctg taatggcgct tcatgcattt
    23281 cacggataca acgggcatcg gtgatatttt cccggtaatg tgtggtttcc agcatcatat
    23341 gatgcatatg gagcatccgg gccagggctg cgcggagttc ttcaatatgt tgttcatggg
    23401 aatgctggcg gggctgtatc cggcagagag ggataacctc cacatcatcc gggtgcgcgc
    23461 gaagccacgc gtcagtgact gcaatgtcgt cagtgtaaaa ccatgtgccg gatgtcccgt
    23521 tggccagaaa acgcattttc caggcttctg gggtggaggt tgtgttgtta tctttcatgg
    23581 tgccaccact cattcaacag gcttaatgct gtccaggcgc agtctgctca gactgtcgtg
    23641 gctgccacag cggcgtcctg tacttttgct gtagcactcc tgccccctga tacaccatgc
    23701 cgttggcgtt tcgtagaggc tgacacgtcg ggttttatct ttaatgatga gaagcccggt
    23761 gtgagttttt gtctttttca tcggtggggt acaaacgcag aaattttgtt gtcattacat
    23821 catcagtttt agcctgcaga gacgctgaaa aatcagcgtt ttcagaccct gttcaggtta
    23881 gtgaatattc ttgcctggcc tttcggcaat atcaatcccc tgaagttgca tggatatccc
    23941 atgcccattg agcatctggc tgtccatgga atggataccg catgtcatca tcaggttgta
    24001 acaaagcgta ctcagcatgg cgagtgcctc ttctgaggat ttacaatgtg tgttcataag
    24061 ggccagaaac tgctcaacaa catccgggtt aatgctggta ctgtgtattt catccttcat
    24121 gatgtgtcct tgttttttat tattttgcat ttcagaaaat aacacgatgc ctgcagcccg
    24181 gaaaacagat gtgtgcacac gtagtcactt tccggcttta tttctgtccc tgacacaaaa
    24241 tacgtggtgg catgaaaagg attgtggcgg actggaaaac gattagaata gcggcgggcg
    24301 cttgaggctg tatgccgaaa gcgttttgtg gacggcatgt agcagaaagc cccaggcgat
    24361 ttttttatca atcaacccag ggcccactgt aatgcctcaa caacattata gtagcccgat
    24421 gacggccgaa aggcaatgga ggggctgcga tgccacaacg aacgttttta atgatgttaa
    24481 tcgtcgtctg tgtgacgata ctgtgttttg tctggatggt gagggattcg ctttgcggat
    24541 ttcgtatcga gcagggaaat acggtgcttg tggcaacgtt agcctacgaa gttaaacgtt
    24601 aacgggcaac acggcggtag agaatctgcc gccggattgt tgcgaaggct gacctcaatg
    24661 cgccttgttt gtctttttta ccggacgact gctggttgct gctgtccggc agctattatt
    24721 ttttccgtcc tgtgcgtcat caattatgac agtcgctctc atttagttgt ctctggttag
    24781 atgtattatt agatattttc ggggtaattc gataaaagtt atccacttca gcaccagact
    24841 gaatgatata tgcaataaga tctaaacgat gaatctcgta tcgtgtacgt aactccatca
    24901 tttcacgcca tgctaaaatc cttgatgttt tcagttcagc ctctataagt ttccatgtgt
    24961 ttgttgagat ggcatgttct tccagctgac gtaacgagaa cctatcacta atgaccaacc
    25021 atccccatcc tcgagtatgt gcccatttgc gacctgcatt actttttatt ctcgtgagac
    25081 tcaaggtcat tcggtctgtt ggcttcactt ccataagtag gcaccgacca tcaactgtcg
    25141 caatgagtaa atctggatag tatttttttg atttgttttt aaaacaatat ggaatagaaa
    25201 atggttgttc ttgatagaaa agaacctgat cgcaacattc taatagattc attattttaa
    25261 actcaagccc tgattcgtac tggactgtac gacctaattt ttgagagtaa aagaatccag
    25321 atatgtcagt atcattaacc gctctctgtg aacatagctg ttctacgggg ggaggaggtg
    25381 cgatatattt tggccaatgt gtattgtcta gccatttact gaatatgaac tcaactcttt
    25441 ctttcttgat tcgggctctt tcctgagcgc gagcgtgtgc taattctgct cttcgttcac
    25501 gtaacctctg cttttgtaat tgtgatatta atgagagcga actttttatt tcatctcgtc
    25561 ttgctttagg aatgcctgcg gtaaaaataa tgaatttggc ggctaagtca gaggatgtaa
    25621 cgaaatcccg atgaacaata cgataaatcc atactgctat atcatatggt gaattaccta
    25681 ttgagttaat aagggcttta aggcaagctc caggtgtccc ttcatagcga gatttagcag
    25741 ctagtaagcc aagggctcgt tcctgaagtt gtcttaccct ttcacgggaa attcctaatt
    25801 tgacggcaat atctgccaga gaatgaggtg aatcatctgt gccgagtcgc attagaagaa
    25861 tatattgatg tcgtttatta gataaacttt cgatagcaga atgaattagc ataatgacat
    25921 catgttcatc ggctatataa tctttaaaat ctgtattggg ttggcagagg gggagtgggg
    25981 tattttgttt aaaactattc ttttgtattt caactttatc atgagcaatt gtttttgcag
    26041 tgctcaatga tattaaatta ttgatgatac tattgtgttc ttggcgagga atcttgttga
    26101 agtaattgct gagatcattc accgtgttta tatcgttgaa ctcaagaaac tcagaaggaa
    26161 gcaggcgttt gattgctcca cgtattcccc cggacgatcg ttgatgtgtt tgagtaagtt
    26221 tttttagcga gacgccctcc gctatggata cgagaagacg ctgtaactct tcagtattcc
    26281 atggagtacc atgtcgtgct ggaaaattgc tgcgggattc agacatgatt ttatctttat
    26341 gttttctagg tactgccttg agtgtgtgga tatgataatt atttataatg atattgttat
    26401 cttttataga taactgatat gcagaattgt attgattttg tctacagtgg gcaagacagt
    26461 gctaagaatt tgtggggaaa acatagagac tccatgcggc gtcctgagat agacggcaga
    26521 caggcattct gcctgaaaag aaaaagccgg ttcagagaaa accggctgac ggattatcgt
    26581 ctaacagttg atagctacct caaaatactt ctgcaacagg aacaccggca actttttccc
    26641 actctttaac ccgataccgg atcatctgtg ggaaacgttc cttgtactca tatccacgtg
    26701 cagccatggt gtgcatggtg ttggccagcg tccttgctga gatgccggag atctcaagag
    26761 gaatcatcgg cagactcaca taggtggtgt ggatttccag cttgccgtcc tcagcaaccg
    26821 tgcgggtaaa gttgtgaata tccagaccaa tcacatccat cacattgttc ggataaatgc
    26881 gcacatacag catggtgccg tttttaatgt cgatcccgaa ataacaactc tttgagaatg
    26941 acagttgctc gtaatcgctg gtcggcgcaa agggactgct ttcttttatc tgctgaacaa
    27001 cctgctggac tttctttttc ttcgctctgg ttctcagata tagccagagt aatgggagaa
    27061 ttattaatgc ccagcatcca tatgcaaaaa taaaatccat tgtttgagat aaggctgaat
    27121 catgaacaat tagtgttccg aaaaaagcat aaaataataa gcatggtatg ccaaatatga
    27181 aatacgcatt gcgaacaagg taataatatg gccaccagga aggatatctg ctttttgttg
    27241 tactcataat aaacccccat attcacctga atatatttta atctcttcag gtgaatatgg
    27301 caatagtgtt ttattggttt ttcgatattt ttatttattg ttttattccg tctttatcat
    27361 ctccattaac atttttcagt ttttcttcac gaacgccagg tgttctgcgc atattggtat
    27421 tacctccggc aataatatct ttgactgaag aaaccatatt ccccagcata ttaacgccgt
    27481 cacgtccgcc cagaaaatta attacatggt caggcagata agcctgaagc gcaaaaacgg
    27541 ctgcaaccag tgtggtacag actcgggcat aaatcagcaa aaatccagcg atgctgaata
    27601 accccgtaag ggagtccgcc tgcacattaa ccagagccgc gccaaacaac gcattcagaa
    27661 tggtgccgcc ggccacgatg gcgaccgatg caaaaaagaa tccgaacacc atcagcagcg
    27721 gtctgatcat gctgtctatc aggtagatgt aaccgtaagc agcccggctt cccctgtcct
    27781 ggcttgttcc cagatgggtg gcggcccaca gggggccagc agcacatcca atcagtacgc
    27841 tgactatcca gttgccgatg ccggtcatcc agaagataaa cgggataaac ggcaggtaaa
    27901 tggataacga aaagccggca cagaacagca aaaacagcag gaagtagagt ggtggtgcgg
    27961 atgcttccag aaaagccttt gcggcgtcag ataatccgct gacggcattg accagtcgac
    28021 caaaaacgga gttgctgccg gctgtggaaa gtaccctgac ggtggtataa cccgcccata
    28081 tgctttcagt aatacccata gtgtaatcac cgatattttt catttttatc agtggattta
    28141 gttggtccga ggatgttcct gttccagata aggatgtcgc aattttgtct gcaaaatttt
    28201 cacccgacca tactttcatt attatgcttt taggttcagc ggcttccgct gttctgtatt
    28261 cagttgatga agtgtttgtt cccagtgcag gagtatatgg tgaattctgc agctgtgcct
    28321 ggtaagcccc cttgacttcc ttcaggaatt ccgtatcccc ggcttcgccc agtgacgaga
    28381 cgccactcac ggtaggggcg cggttggcca ggtcttccag tcgctggttg gcggtggcaa
    28441 aggtctggta ccaggcaccc agggcaaccc agccgtaggt ggtcaggtag gatttcagct
    28501 gctccctgcg ctcagtttct gcgttatctg ccggcaacgc cttctgcacc gtttcttcat
    28561 accggttggc tgccttctga atacggcttt caatatccgg caggttgccg ttacccgtgt
    28621 cgcgccgggt ccggtatgcc gacacaaatt cctgcgccgc gctgtccagg tcacggagca
    28681 tggtatccat ggcggagcgc tgtgccgtca gaaccccctg atattccgtt tcaatgaagg
    28741 gattgaaaaa atgggccagc gcggactgct tgttactgcc gtttcctttc acggagagac
    28801 tcacactccc gcaggtggcg ctgccatttg agacggtgac ggtatatctg ccattctctg
    28861 tccgtgagga ttcgctcatg ggcggcgtgg atgaacgtgc ggtacggtta aagtcattga
    28921 ggcccgtatt catggcgtgt ttgcacagtt ccatctccag aattccccgt gctgccgtgc
    28981 gggtggagac atgaactggc tgtacggtca ttgaatagcc ttcagcaatg ttttctgccg
    29041 ctgtctgaac catgatattg gcggaaccga tgcccatgat ggatgcgccc cacagcatga
    29101 ccagttgcgc cagactccag ccggaagcgg tgggcacaat catcaaaaat ccactcacga
    29161 cactgagtat gccgacgacg tcccgtccgt tgttaaagac actgccctgg tgtcccgtgc
    29221 ggacagcatg acggatacca ataaacagaa accagactat ccccatcgtg gcgataattg
    29281 cgttaaacac catgaacagg ttgccaatga tggtgttgct gctggctgtc aggggatttg
    29341 tgaccacatc accaaaaatg gtgaccagtg cctggcgcga caggtcatcc ggattggtgg
    29401 cggcactcac aatgtcctga taggtgacga aagcctgtgc aggtacggca gccagcgcca
    29461 gacctgcact cagtgctctg ggtatgattt tcacggtaac tccggaaccg aaggggatgc
    29521 ccgggcatca gcccccggaa tgaggaaaag aaattgcaga ccggacagtc agtactgccg
    29581 gtgggtaatg gccagccatg tggcctgttt ccagccgtgc cgggcctgca ggtaatcggg
    29641 gaatgtgccc tgttcgtcct cgctgacccg acgttcattc agctgccaga ggcggtatga
    29701 cgcaatcagg gcttttgagg cacacagtgc tgcaatggtg ctcatcagta acaggcagac
    29761 caggatacgt atcagggtga tgaccggcag tgcggtggcc gcaagggcca taccacacag
    29821 cagaagtacc ggtatcatca tcagccagaa tcctgtccgc cagaacatcc tgcgacgtcg
    29881 cagacgccgg tccagttcgt cgaccgtcag tccgctggcc ctgaccgctt cctcccaggt
    29941 catacagacc ggactctccg gttgccggtc gggcatgagg cgcttcaggc gcgttttatg
    30001 ctggtccagc atatggcggc agctgcgcag catccagcgg gtttcggcca gtggtgcgaa
    30061 cagatttatc cctatatcac cgatgcggcg gagacggggg gccggttttt tttcgtcgat
    30121 gctgttgtcg tctttcatct tacgctcccc tggctcatgg cctgtgacag ttcgccaagg
    30181 cgctgtccgt aatactgccg tgcggccagc gcaagttgct gcccggcgat aacgttaccc
    30241 tgacgaatct gctctttcag atcgttcatc tgccagttca gcagggcggt ggtgcgcagc
    30301 agctcacgga gaaggttatc gccttccatg ccctgcagat cctgctgcca gtcggtgttg
    30361 gcataccgtc tgccggcttc aaaggcctca aactcccgct ggctcatgtg gccacgggtg
    30421 cgggcttctg atgatgcggt ccggtcaaaa tatgccgcag ctgacggtga ctgcagggct
    30481 tcagtcagtg ccccacgggt ggcttcatta ggggtactgt cagcaatcag tgacagctgc
    30541 ggatgagagg cggcatcgat gatgccgttg tattcattct gcaggcccac atacgtgcgc
    30601 ccggtctgtg tgcccacttc tcctttcccc ggggcacgac ccgctgacgg tctggcggtg
    30661 tttttcatat aagccgtcgc ggcatcaatc tgtttctgat cccaggtgag agcggccggg
    30721 gtatctgctg tgcctgcgcc ctgatagagc gatcgtacct ggctgtctcc gcccggcaga
    30781 tcgcccacgg cagggcagac atcagttccg ccaaagcggg catactccgc ttccgtgcag
    30841 taaccggcgt ggatgccggc actgtcatac gcctcgcgga ccggggagtc cgcagcccgg
    30901 gagagacgtt cccggatggg tttactgctg acccctcccc cctgactcag acgtgccgct
    30961 gcagacgctg ctgcccggcg gctttctgct gcaatgccgg acgcggattc actgcagatg
    31021 gagtccggca cgctgaagct tttcctggcc tcctcgaggc ggtgtgtttc attactgaat
    31081 atcatctgct gacgggtggc ctgcccgtcc tgggcaatga cggtggccag tttttctgag
    31141 ttctgggtga tggctgtgcc ggtggccgtc tgcatacgga ccatttcctc catgatgccc
    31201 tgtatggtgg ccagctccgg catgatctgt cgggtgaccg gctgactgga cacaacattc
    31261 acagagtagg catgggtggc agggataacc gcacagccta caaggagtgc aagcagtgtg
    31321 gttgcagatt gtctttgcat tcttttttct ccgttttcgg gggatacagg atgtctggta
    31381 cgcgatgact gagtgattcc ggcagcggag ccagcgggaa acactggaag cggcgcgtga
    31441 tccatccctg acggttacgg ttatcgatga gcgtgaaggt acgacgcttc acgcgtttca
    31501 gtgtcggggt gtcgctttcc atgaccggcc agtctccggc tgtgagtgcc ggtgtgccgg
    31561 ggacatccgg ccacagccag gtgttgtcag acatcagcgc agtgagtggt gcgggcagaa
    31621 gccagtgaat gacatgctgt atatcaaaag gcggtcgctc cggcacgtta acctgccagg
    31681 tggccgttgt tatcagcgtg cctgtctctc cgggcagggc caggcgaata ccactcatct
    31741 ccccgtcagc ccgtctgtca ctggcaaaaa tgacccgggg attgggggac ggtcgcagtg
    31801 tgaagcaggc ggacggcagg gtgaagctga aactgtcacc gtccacgcgc agtgttttca
    31861 ggaagacctg tccccgggca ggacgccggc agcggggggt gatatgaatg ctgtacatca
    31921 gaggttatat ccctgcttac ggatgagttc tgctgccagc tcatggatga cgttttcact
    31981 gtcatgtgtg cgggcgcggt gttctatcag tgaggtggct gaaccccggg gaaagtgttc
    32041 ggccaggatc tggcgtgccc gcagtgaccc cacctcctgc gtcagcgcac gtcgcagggc
    32101 gctgtcttta ggagaggagt tcagtgccca cagctccagt ggaccgaggg tgaatttcag
    32161 gatccgggcc agcgtgccgt tttttacacg gaacacagcc agtaccggga caccgctgcc
    32221 gtccggtgcg gccccttccg gcattttcag gaagcgccgc agtgtgactt ccgggacgcc
    32281 aaagttatcc cgcaggaacg gaatttcgtc agggcggtaa cgcagcagcc acagggtgtt
    32341 ggcggatttg agcacggccg gaggataatg ctgcagcagc tgggtgctca gcaccgtgcg
    32401 tatcccgaac ttacgctgct ccagttcctg ggtgtccagg gcttcccaga tgaagttgat
    32461 cccttttgca ttgtgcagtt cgtcatagac tttggtcttg acctcctgat caagctgttc
    32521 aatgcggcgg aaaatcacct catggtagcg tgggtcgaga tttttccgta tttcctcctg
    32581 atactgcggc aggacaaaat ccccgccggc gatctgcccg gccagcagat acatgatacc
    32641 ggtgcgcaga cgtccggcag gtgtcttgtc cccggcaaca ttgttcagat caacggcgac
    32701 cacgcgggtg tcggggctga gcataaaccg ggtacagccg gacatcatcg ggtagtcact
    32761 gtgtccctga tcgagggcac ggcggatgta gtccagcaac cgctcttccg agttatcacg
    32821 ctgaacggtg ccgaacacat cccggatgga cggctgtccc agcagggcgg acatttcagc
    32881 cagcaggggc atggcctggt agtgtgcccg ctgggcggcg gggatattcc ctgcaatatg
    32941 cagcatgtcc ctgatttcaa accaggtggc ggcattccac catccggcat catgttgttc
    33001 agcgatgccg ctctcttcca gggcgagatc caccaggggt tccgtacctg cgcggtaaag
    33061 tctggggtta ttctcgccat attcccggaa ggccaggtcc accaggctgc tgatgatttg
    33121 ccgggtgtcg ccgggattac agggctgacc ggtgccggta tccacacaca atgcacagag
    33181 gacggagacc atgaagttct tttccggtgt gacgggtttt ctggccccgt acatcacatc
    33241 aaacaggttg cgggtgtggt ccgggtcatt actcagaatg atccccacgg cttcgtcctt
    33301 tctctgctca ggcaggctgt cacggataag ctgtaccagt ccctgtgcac tgaatccctt
    33361 gtcgatatag gcaatgaaag ggagattctt ctgggctgag gctatctgga tctccgacag
    33421 tgcgttgatg agtaccgatt tgcccagacc gggtgcgccg ggtgtcagct ccgtgtgctt
    33481 gttctgttgt gatgacgcca gcgccacctc ccatgcagag ccgtcttcgg tgtgcatcat
    33541 cagatttccc tgtccccgcc agacagagcc tgccctgttc aggggaaaca gggaaagggc
    33601 gtgtgaaagg ggcgggtaaa gggggatcgg gccggagctg tgggaggctg ccagaaccgt
    33661 gttcacccag gcacgtctcg gatcaccgaa ggtggtggtg gtgccgcata ccccccagcc
    33721 ttccagcgca gatttcagga gagcctggtt acgggtacag acttcccggc tgtttcccca
    33781 tgtggtggca acaatggtca tgatacagac cggatcatgc ttgttgatgt cattcagcat
    33841 cattaccgag tcatacatgg gccggagagg aggaataaag ctgctgtagg tcagcagggt
    33901 tttcttgacc cccagcgcct tcatgccgtc gggcatcaga tccatccgga tacgccaggg
    33961 gattgcacgg ggcacatccc tgaccagctg gttgaatgtc cgcagattct gagggggcag
    34021 ggtaatggcg accataccgt gccagagctc accggcctga atcaggctgc cgtgtgtttc
    34081 aggctgtgtg gtgaataact gcagattcag cgacggtgcg tggaatacag aggtatcccc
    34141 gccctgtcgc cagccggcag gacgggcatc ttcgggcaga tgaggctgcc atactgccgg
    34201 tgtactgttg cgctccagct ggcggcggat ttcccgtccc acttcatgga tatccatcag
    34261 gcgaatcagc aggccatcag aatcatgtgt cagagcctgc tccagcgtgt ccagtaaggc
    34321 gtcatgccgt attttgaggg cggaaagtgt ccagcgccag ggatcctgcg caaaacgggc
    34381 tggcggagcg tgttcagcga gtttcctgac atgggtctga tggttcttgc gatcggtccg
    34441 gctcagcagt tcttcagagc tccagacggt cagccagcag cgctcccgga caagccaggg
    34501 ggtgagtgtg gtgacttttt catccaggac gtcctgaagc aggatccccg tgcgtgcgat
    34561 ggattttcgc tggggtaaga gcatggcttc gatttcatcc cttccacgct ccggatcgcg
    34621 ttcgaaaaca aaactgattt tgtggccgga atttttccag gccgtgttca ttcgctcagc
    34681 cagtgctgca atcagtgact ccagtgtccc gggggccgtc tggtccgggt tttcatccat
    34741 ttctttcagg gcaccgctga tttcaaagac ggaaaggtaa tacccctgtt ttgtggtggc
    34801 aatgtagggg gctttcagcc aggggcggcg ttcgcggtcc tgctggtcca gaccgatgac
    34861 ggtgtgcaga tcacagtaat cggggaagaa tgcggaaacg gaataccgtg acagggtact
    34921 gatacagtct tcaatggcaa aaataatgcg gttgatattc atggcatggg gcactccggt
    34981 aaggatgaat catcacggtc ttatattttt tgatgccaca gcaggggatg tggcatgaaa
    35041 aaacagcaga gaagataaat aatatgttga ggggcagggg cgtatttctg tgtgattaca
    35101 cccggataaa taaactgctg gcggcgaatg aaccgggcaa tctctttttc atctccgtca
    35161 ggtggaatat cgtcagtcag cgaccggata ataaagtgcc ggaatgcatc tgcggagaac
    35221 tgatatccgg caaaacgtca ccggcaccgg tgtgtgtcac atgacgtact ttttgtcagg
    35281 aaacgaatcc ggcagcagaa tgagcgccac cccgtccatt ttctccctca acgttttacg
    35341 ctgtgccggc ggccagcggc gcagggcatc agcaaaatcc gccggacgcc aggtgcggaa
    35401 tttctcctca acggccagac ggttctgtac cagggcattc aggactgccc gtccctgcct
    35461 gacccgctga cggttatcgc tgaaaatctc ctgcagtgcc cgcgcattga gggcaaccac
    35521 cgaggccgga tgcaaatcgg ggagccagac catcactccc tgtgcggcag catagccgtt
    35581 cagacagtgc caggcatggc agaaggcaca gagatgaaag ggcgggcctt ccggctgcag
    35641 caggtcactt tcattttcgc tgagtgcatc cgttgtgacg atttgcgact tttccacatg
    35701 acgcagggcc agtcgcctgg ccgacaggcg ggcctgtgcc agggaccgct ccgcatcctg
    35761 cagggttcgg gtgtgtttct ttttttcgtc agcgttttcc ggtaccggac gttttttcag
    35821 gcgcatcacc cgggtgaggt ttttttccag caggacgacc gtatcccggc aggtgtcagc
    35881 catctgctgt aatggccatg aatcttccag aatgaagcgg tgggtgacgg tcctgcgaca
    35941 gcaggggcag cgtccctctg ttgagagcac tggcggtggt aacagggctt caccgctgaa
    36001 taccgtgtcc ggttgcggga gcgggaaaag ccatcgttcc cggaccgggg cgatgagtgg
    36061 cggggtcttt ttcatacgct tttctctctg tatacagggt gacggcagcc agaatcagtg
    36121 ctgctatagc ggtggagtaa cactctgcaa acacaaactc ctgattatcc cggttgtcgc
    36181 gggcaatcac gttgccgtgc tctgttctga gcagggtgaa agggggtagc tcacaggaca
    36241 tggtctggtc ctcctcattg tgtgaagacg gcaccggttt cccggtgcct ttgcggatta
    36301 actgattgaa accggagaaa gaccgagcgt tttctgtccg ctgcccatca gttccggtac
    36361 gacggcaagg cagacgccga ccagaattga cagcaggcag gccagccctg caccgggttt
    36421 tttaccgatt tgtttgaggc caagcagtcc gccgataaag agcaccaggc caataacctg
    36481 accgatagcc acaaacgaac tgctggagcc tctggcacca tcgccaacgg ttttaatcat
    36541 atcgaagagg gtatttgcca ttgcggtgtg gggcagcagc atcacgggaa gcagcagtaa
    36601 caggcgtacc agccggtcac ggcaggccat caggcataac tgaacataaa cagatgcagc
    36661 gcgcagacgc gccgccatgg cacgaagtcg tgacatagtg tctcctttat taacagattg
    36721 atttactgaa aaaatacagg ggtcagaagg cgataccgag gcttgccaga gtggcatcga
    36781 taacccggtc gttgaaaacc agcagaacgc cggccacaaa cttgacgttc cctttactga
    36841 tggattcact ggcgctcagg gcggtgtgcc cgtccagcag tgatttgcgc cagatggata
    36901 tgccgttcat gacaaaaccg acgccggcca gttttgccag tgagagaatg gcattgacgg
    36961 caccggcagc ggcaccaaag gagcccggct gcacgtatgc gatgggggcg aaagaatctg
    37021 ccctgaagcc catctgggta ccaccggcat tgatcattga gggcagactg accagcatgc
    37081 cgcagatgaa gataccggca ataaattttc ccggtcccat actgtgacgt ccttctgccc
    37141 gggcgcgcat cagatgaccg ataaggccga taatccccag cagctctccc caggtaaaga
    37201 gaaactccat gcctgcactg aaaataccgc tggcaaagtt ctctatggct gttagggcat
    37261 caatgctcat catcgtaatg taccggcaga ggtgatgact gcccggctgg acgggtcaat
    37321 ccgggtgatg gtgatgtttc ccagactgtc gccttcccgt actgcccagg cactgccctg
    37381 ccagcggatc caggccatgc ctttctccac cgacatgatt ttcatgccct gcagaggtga
    37441 aggcagggtt ttcgcaggct gtctgttatt cccggtcggg gctggcagtg ctccctgcat
    37501 ctgcagggta tgcacctgct tacgggtctc ctcaagcaac tgacgggttt cctgtgccca
    37561 ggcctcatcc ttctgaatgg cgttactgag ggtggtgata ctgtccttca gcatggtcag
    37621 ggtgcgccgg atttcctcat cccgtctgtc cagctctgct ttcagatcac gggcaagttg
    37681 ctgcatgtct gcctgtgggg taaccggcgg tgcgggcatg ttgtttccgc tcccggactc
    37741 cgtcatggtc atgggctctg cccagctctc agcagcgggc ggtgtgacag caaagcctgc
    37801 atctgcaggg ggctctgagg gtgtccgcag aaacttgtac cagatggcga ccaggatgat
    37861 gagggcacag ccggcccaga ccggcaggct gtatcccata tactggcgtt tcaggaagga
    37921 tttgccggat gccggtgccg tcgtttcgcc aaagctgccg ggatcttcat gttcgttttt
    37981 catggataac tttcctgttg tatgccgtag cgttgctgca tttcattctg tttacggagg
    38041 accgccgccc ggatcctgtc agaggcctgg tgctctgcct ccgcctgggt gagcggacgt
    38101 ccggaggagg gggactcaga aggggcagtg ccggaggacg gcgtactgat atcgttgctc
    38161 atgaccggct caacaaacag aatggagacg acctcgcccc gtgatactgt cacctgacgg
    38221 taaggttcgc tctctgcctg gcgggtcagc agctggctgc cccgttcggc catgccacct
    38281 gcaatgacgc cggccagcgc ctctccatca ggcattccca ccctgccggt aatactgccg
    38341 tagtttccct gaaggatctg tgtgttggcg tctttgtaaa gacttccgac attgcccacc
    38401 ccgccaagta cggacggcag aacgatgtgc ttaaaccagc ggtggttcac gtcgctggcc
    38461 acggttgaca ttcctgttcg ttcattcagg gcatatgcat tcaccttcag aaacatgccc
    38521 cgccacatca tccggtcaaa gtggatatcc attccctgac ctgcgagtct ggcgtcctgg
    38581 ctgtagagaa cggctccggc ccatggcccg gcaggtacgg aggccaccac gcgggagacc
    38641 gcattgtcag agtccacttc ggtgtcgatg taagccggta cgcgggtcag tgccggtatc
    38701 agctggatcc cctgacgtgt agccggtgtt gccagactcg tctcctgcag acggggatgc
    38761 tgtgatgtaa cggtaccgga cggtgacgca tgaggggctg tcccgggcgc gccggtggtg
    38821 ctccacatgg cagctgccac taccggtggc cgggcatcct gatgctgtgt gttgatacgg
    38881 gcaagcagtt tctgtaatgc ctcacgccgt ttttcccggg ccgtctgatc ttcctgagga
    38941 ggggatgcgt tttgcccgga agtgcctgac tgatcatcgg ggggattatc ccttttctga
    39001 tcgtttccgg tatcggtatc cagtccccgg ggcagactgg cgataaacgt ctcgttatta
    39061 cgtaacgctt ctgcagcacc acgttcattg tcagcccgca gtaaatcccg gtagtgctgt
    39121 gtctccatca cgttccgtcc gccgccggac gttgtgctgt tgatattgac tgacgagagc
    39181 gatgcagcgt tttcggggga cagccaggtg agcagcaggt atccgccccc gatgataatc
    39241 acgcccccca taaccagcag ggctgacagc tttttgccgg attttgcggc tgtgtcctgt
    39301 tcttgttctg cgctcatgat ttactccagt tccggggtga cgtgaacgga ctgaccattc
    39361 acggagaagg tgagtaaggg ggtcaccggc agtttccaca gccgcgtacc atcagccgat
    39421 gccagcgtct gttcaaattc atcccgcagc atggcccgtg agcgcacgag aagatcatcg
    39481 ccgtgttgcc agatggtggt gtccgggacg ttaccggaga atttcaggcg gcggatggcg
    39541 ggatcatccg gtggcacacc gtccagaaag gcctgcagag tggcgtcatg cagggcaatt
    39601 ttttctgacg gtatggacac caccgggctg gcaggtccct gccggggaat gcgcagatcc
    39661 agacgactgt cgatttcctg actcagggta gtggtgtccg tttcaccact ggtgatattc
    39721 aggatgaccg gtacagacaa tcctttgaga tagaccgtca cattcccggc agcccaggcg
    39781 cgcagggggg tgacggacac catattgccg ttgaactgca cgtcaaagtg accgggcgca
    39841 gcgttatagg gcggatccga ctgtggccac ggactgccgt tgacgtccgt gaaggtcacc
    39901 acgctgacgt tatgcagagc ggtacggaca aggggcaggc tggcccccgg agacagattc
    39961 accgtgaggg aactgatacg gggcaccaca ctggtgagcg gggcactgat ggcccgttcg
    40021 ttatcggcca ttatcccacg gagttcacga atttcactgg cattcagggg agaatccagc
    40081 gatgcgcctt cctgtacaag agcagcgccg ggaggcggca ggtttcccgg gagttgtgcg
    40141 gctggcgggg gaccgcctgc cggggtgttc ccggttgcct gtgcggtctt tccgccggtc
    40201 tgcgggcttc gtgcattttt ccagcccggg ttatctgcag ctgtgacagt gccgcacaca
    40261 ataagcagtg cgccaataag atgatgtatg tgcatgatat gagtctcaga ttcctgctgc
    40321 ggcgttacgg gaaaccatct ggctgatttc catgccgccg ggtttgatgc gggggtcaac
    40381 gcgccggaca gtgatttcaa aggtgaaggc ctgttcgggg cgggtgctgg tctgaccgac
    40441 caggcgcatc ctgaccggat actggaattt ccagaaccag acgccgttct ccatctgtcc
    40501 ctggttgacc agggtacctg ccccgatagt ggttgtgagg ttaaatttct cctttttaat
    40561 agtgtcgaga atgttcgagg cctgaagggc attcacatag ccggcaaagc cggcttcgga
    40621 aaagcggggg gcctgtgtcg tgatttgttt tcggtagtgg acaaaatcga gattgaaggc
    40681 ttcgctcagc gtcatgctgc caaagttcat gacgtcgctc tgactccagg ccggctggct
    40741 ggttggtgcc agacggacca gtcggccatt gtcagtggca aagtattcgc gcgtcacgcc
    40801 ggtggcatgc cagtactgta tgccattagc caccaggctg atgcagagtg ctgcgccgct
    40861 gtacagcaac gccctgacca gaccgggtac cagcgcggcc cgttgttctg actgtgcgat
    40921 ggttctgagc acggggttat aaatatctcc gctgttatcc gcttccggtg agacagggga
    40981 ggtgggttgt gcttcgctca tggtgctcct gttcagaggt tgtagtcttt ccaggggtcg
    41041 atattccccg gaagatggtc attcacccga tccacgacgg catcacagac ctggttttcc
    41101 aggtcgcgga ggagcttgtc caggctgggg aactgcggaa gagtgatact gatgtccttg
    41161 atgcgggaca ggcaggactg cagggaggag gcgacgtcat tctcccgctg tgaccatgcc
    41221 tggctggctg tgcgcgcccg ctcccaggcg gctctgctgc cggcttcggc agcggccccc
    41281 gcccggcagg tggcggcacc tgccaccggt gatgccaggg tgatgccggt cagtatgacc
    41341 agcagcgttt ttttcatgtt catggtcgtc cttaccggct catcggccgg ggctgtggga
    41401 ttgtgcctgt tgcagagttt tttcctgcct gtcctccgcc ttttggctgt cagaccactc
    41461 tttccaggtc tgctcaaaac ccagagagcg gaaaagcttg tccggttgct cttccggttt
    41521 gtggatggcg gcaaacaacg gggagtcctg ttgcttcagg tgaaactgca ttatttgtgg
    41581 ggacggacgc tgatgattat tctcatcatg gttaacagct tcaccgtaac cggcaaactg
    41641 cagaccgtta tccgtcaggg cattgataac cagctgacgg ttgtcagctg acggggtggc
    41701 cagtgccggg taaagggtgc cgttcaggcg gaccacgccc tgcagatgat catctgcgct
    41761 gtggaccagc agaagattca actgacgatt gtccttatcg gcagaggcga tggctggtat
    41821 cagttcattg ggggcattac cagcgagggg gagtcgcatt gattgatcct gaggtgctga
    41881 tgcaggttgc ctgtcttcac caatgtacag attattggga ggatttttgg gggagcgaac
    41941 ttcctgcatt atctgtggcc agtgttgttg catctgtaac atcacctggc tgaatgttgc
    42001 catcggaaac tccagggtat gctgacccgc atgaggttgg tcattattaa cggtaatgcc
    42061 gttactgacc ggggttatgg cccagatatt acggatacga tcccgcgttt caccggacag
    42121 ccggaatgtt tctctcgcct gcagtgtcag tgtgacgggg tcacccttct gatggttttt
    42181 gaccagttct tccaggccgt ttccccagta ttcccgtttg ccactgcggt tggtgagctc
    42241 cacaaaaaag cagcgccctt ttccgggcgc atactctttt tctccgtagt ttgtcaggac
    42301 ggcagtcaca ctttcaccgg ggctgagctt gccgggcggc tcttccaccg gtgtgtcggg
    42361 ttgctgcggc tgtgcactga caggggcttt ttctggcgca gacgacgggg cggcggggtg
    42421 ttccggtctg tcggcccggg gggcttcagc ttttgccgtg accggcgctt ccggatgcgg
    42481 tgtttcagcc gtactgcctg ctgtttgttc agcctctgcc ttcggtgaca cagggggagc
    42541 cggttgggcc attgttgtct cttccggtga ctggcggttg agttccggcg tgggtctgtg
    42601 ggtgacaatg gcatcatgcg ccatctcttt ttccttgcgg agtttttcca gtgctgcccg
    42661 ctgggccggg agtttcatgc gaaccttgat gtcgtattcg acaatcagct gcatggcttt
    42721 ttgcttaaag gcatcactgc cggtcagctc gataacgccg ccatagaagt tggtggccac
    42781 agcaagggca gccaggacct ttttgtcatc cgcgctggcg ccatccacca tttcgagaca
    42841 gtagagtctg tccaggaagg cgggttcacc gtcaacgctg tagagcgcag tcctgtcggg
    42901 aaactctttg tgtgtaagat ttttaaggat ctcgtccagg ttgtgccgct gcggactgtc
    42961 gggacggcgg ggtgcaaaga taatcgtgtc cggttccgct gacgatgtgt cttccgggct
    43021 ggtttccggt ggtgatggtg gtgcgacctc ctctgttgct gtttcagcct ccgtgggctt
    43081 tttctcctga ataacggaag gttctgaggt gcgtgatttc acaccggtca gaaaacggcg
    43141 cagacggctg aaaagcgatg gcgtttcagg gcgggctgct tcattcgctg tgctggtctc
    43201 agtggcggac acaagacgct cttcctgcag tttatcttca gccgtcggtt cagcggtggc
    43261 ctgccggact ggctgagttt ctgcaggttc agaaggtgag gctgtttctg ctgtgccggt
    43321 gttgtgaaca gcaaccgtgg catcggcatt gagctcctca gcgggtgcgt tcctgttgtc
    43381 atcggtgatg tcagaggagt gctctgcgcc ggtctcagcg gcagacgcaa cgctcttttc
    43441 ctgtgcttta tcttctgccg tcagttcagt ggtcacctgc tgaactggcc gcatcccggc
    43501 cggttcagaa actgtgtttg tctccgcgtt gtcggtgttg tgaacagtgg gggcccccgg
    43561 agtgtcagcg ttgagatctt ccgcaaatgc gctccagtcg tcagcgctgg ccatggaaac
    43621 ctgcacttgt tcttcagact gcgttgtggt ggtgtgctgt gtcccggccg cgtcggagag
    43681 ggaggtttcc ggtgtgtctt cactccgggg atgttcttct gcctgcgtgc tctggttgtc
    43741 agcggtggtg gcagaggcat tttcttcacc ggtatcagcg gtagccacag aggcattttc
    43801 ttctctggta tcggcagcgg aaacaacgtt ctcgtgtggt ttatcttctg cggtcggttc
    43861 agtggttgcc tgctgcacgg actgcatccc ggcaggttca ggaagcgttc ctgtttctgc
    43921 tgtgtcgctg ttgtgagcgg tgtctgtacc tgtggcgtct gtattgaggt cttcctggtc
    43981 gccagcggta ggcacagagg tattctctgt gccggtatca gcggcagacg caatattctc
    44041 cagtggttta tccggggata acgtctccgc agttactgat tgcagggatg cataggcggc
    44101 atagtcatct tcatatgcct ggttttctgt atcgtcggag tgggtgtcat aaggcgtatc
    44161 ctgtgtcgcc agctccgttt ccggggctgt gctctgtctg ttttcagggg acatttgtgg
    44221 cttctcatct tttaccggtg tgaatatcag gttgtcgatc acgacagcct cacgatggtc
    44281 ggcagtgtca ctgttgggtg tggggatacc gatatgtttt aactgtgcat tcatggcatt
    44341 ctgaaagttg tcccgtcccc ggctggcgtc caggtcattg aaatcggtca gtccctgtgc
    44401 tttttccgcc tccgtaaagg ccggaataat gacttcgcca ttcgtgactt ctgcggcctt
    44461 tgttgcacta aaaacccctt tgttaatttc ccgttgatga tcgttgtcgg cacagaagac
    44521 aaaggggctg tcagtccaga tttggcgggc attttctgcc acggcaatca tattgctggc
    44581 atcaactgtc atcagaaccg gaaggccggt tgcttcgtgc agggaggcgg cagtagcgta
    44641 accttccgca aataacaggg ggcggccatt ttccggggtt ccgaaggtga accagttgcc
    44701 ggttttctcg gagtctttca gaatgcgggc atccttcccg ccggtgaccg gaatacgctg
    44761 gtacgaacgt atttctcccc ggccgttgct gaagggaata accagctcat ttttattgtt
    44821 cagccgcacg ccaggcgcgg cccggatgcc cttgcgcgtc aggtattcat gtgctgttgc
    44881 ctgtggcaac ctgttaatat aggagcgggc atagccggcc tgtttgttat actgttgctg
    44941 cagtttacga gcgttatcat cgcgctgttg ctgagcgaat gcccgcagat gcagtctggc
    45001 cagaggatcg tgttgttctc cgccggaaaa tacccagttt gtgggactgt catcggcgct
    45061 ccggtagtcg cggtaccagc cggcgggacg accatcaaga taggccctgt atgcgccact
    45121 tttggcccct tttttgtcat cccgggtggc cactctgtgt atggctccgt ccatctgtgg
    45181 caatccctga ataaccagcc cggcgttttc caggacctga gcaaattcag tgaccggatc
    45241 gccggcatcc atcgacagtt cctgtggccg gggtaaccag cgctccagca gtgaaagttc
    45301 ggtacccgcc cgggcatacc agagcttttt ttctggatcc catgcgacag ctttgcggcc
    45361 atttaacggg ggaactgcat tttttacggc ttctgtctcc tcaggcggga cagcaagcca
    45421 ggttcgatat ctggacttca tgtcgttctc cggggattaa agatgagggc gttgtgatac
    45481 gccgggatga tgaacaaaag agggttattc cgttccgcgc cattcacggg gtaattctgt
    45541 gcgatgtcgg taccaccagg gacggcctga cgcctgttta ccctgcacca ggctgtacag
    45601 acgccggcag agcgtccgga cagtccatcc tgagtacccc aggagcacaa aaaacagcaa
    45661 aatgccgctg cacacccaga atgttgtttt tgacggatac ggaaacagca gcaggtaaat
    45721 catggtaaac ggcaacggga caccataaat attcagtggc ggagtgacat cccgccagaa
    45781 gccttcgcgt ttcatttggg gaattctccg gccgggatga agagcgcagc ttctgatgcc
    45841 tcaatgctgc ccgtctgata catggtcaga acctgatccc gtatccgccg tttttccttg
    45901 cgaatgattt catcaatgta atgcccccat gtcgcatggg actgccggga gagcgtatca
    45961 cgcagttcgt cagttatgac gatgtattcc cggacggcct gtctgccacc gtcatttgtt
    46021 ctgaccagga cctgcaccag aatgaactgc accagggaaa gggtggcggc tgccattgaa
    46081 tccctgattt ccggtggaaa taaccccagc agccgggcaa aggtttcacc gggtgatttg
    46141 gtgtgcatgg tgccgatgca gaagtgaccg gtgttacctg cctggacggc tgcatctgcc
    46201 gtttcataat ccctgatttc accgataccg atgatgtccg ggttacggcg aacggcagag
    46261 cgaagtcctg ccgcaaaact ttccacatca cgcccgattt cggcctgatg gggtggcaga
    46321 aggtcattct ctccccccag aatgtattcc accggatctt cataggtcac cgttttgcgg
    46381 tcggggaaat gcgtctgaat gtagcggtac agtgccgctg ccagtgtgga tttaccggag
    46441 ccggtctctc cgcagatgaa acccagaccg gttttccgga gcatggcatc cagcaggtcc
    46501 ggttcaatat tcatggtgag gatgtccgga atatcggacg ggatcacccg catggtgacg
    46561 gagattactt tttcttctgt tgctgatgtt ccctgaatca ggtgacagcg caggcgaaca
    46621 cgctgccccc gttgcagacc gtagcgaccg gaggcatcac cgctgacctg aatggtacgg
    46681 tccacgggat gaccggacag cacccgggga aggacttccc gtccaaacaa ttcatcactc
    46741 agcgccatca tgatggagga cggcagagga tgtgcggacg aacgtacccg ccgtccaaag
    46801 cgactgacag ataccggtga gccgcctgtc agatcgatat cactgacgcg gtgtcgggca
    46861 caccaggcaa aaaaatagcg aaactcttcg gccgtgaaac gggcgaaagg gaatgtgccg
    46921 aactcatcgg gtaatttcat cgggggctcc ttccgtgctg acgaccggac gccagcgtga
    46981 tttctgcagg gtgaattccg cgtgtgtctt cagacggcgg acgtggtctc cggtgatgag
    47041 tttcctgccg ttaccggtga cggtctgccg ctgttcagtc acatccgggc gtgtgatcat
    47101 gccctgacgc cagagcaggg cataaagcat cattccccgg taatcccggg tcaggcgttt
    47161 ctggttggct tccagggtga ggtcagcgtt gtcacggcct tcctgccagc cctggcggag
    47221 tgcgttttcc cagagtctgc gctgtgcgcg gtcttccggt acaacacggc cctcagttcc
    47281 cggggtggcg gtagtggaaa gcccccgcag aagccagtca cgccagcccg gcgggttact
    47341 gacaaaacgt tcggggcgca ggatggtcca gacacgggac gcggttctta tctggtcagg
    47401 ctgaatgtga gcaacatcct gcgcttcatc aataacaggg ggcagccagc cttccctgct
    47461 gataagggga cggaagtcat acagggcatt cagtgtactt tcccgggcat tcagtgcctg
    47521 aatcagttcc catgagcgct gtgcttttcc gccacggaat cccagcgttc gtccggcgtc
    47581 cgtgagcatc tggcgacggg tgtctgaaat gccgtcatcc tggttgtcag tgggcgtcag
    47641 ccatgcccgg agatccggcg gtggtaaatt gcctgccata ccggcagggg ggcgatggtg
    47701 ctgtccggca cagcctgcca gcaggagccc ggacagcagg agacagacct gttttttcat
    47761 tttttctccg ttggcatata ggaaagatgt aaacgcccgg ctgactgtga gaggactgcc
    47821 cgccaggcag tctgggcctc tgtgagacga agggcatcag cgaaggtcat atcgcgtaca
    47881 tggagtgaga cgggcagggg aagacgcacg ccgctgtaac tgaactgttg tccgcgcaga
    47941 cgggccagct cgctcaggag ctccagagca tcgccttccc agtcaaagga aacccgcccg
    48001 ctgtgagcct gcaggctgcc tccgggctgg cgggaaggca gggctgcaga atacgtgccc
    48061 tgctcccaca actgatactg tgcatgtcgt gtgacagcga cttcagaagc tgcaccgtac
    48121 ttcctggccg gtgcggtatt atccggggga agatgacagc cgctcagaca gagcatggca
    48181 actccccctg caagggtgat acgttttttt tgcatggttt tatccggaaa ataatgaaca
    48241 ggactggcgg tgagtggcca gaagagcgga aacgagcaga ttcaggtcag aagccccggg
    48301 cgtcccgggg cgtgataatc agaaggtgaa ctgtaactga gcaatggcac cgtagttgtt
    48361 gtcggtgccc acgaggccgg cgacatcaat gtgaatcaca tcaaacggtg agagcccaat
    48421 cccggcagtg aatgcactgc cgttgttgct gaccagattc tggcggtaac cggtacgtaa
    48481 ctgtgcccat ttccaggcat taaactcagc accaacggcg gcaaactgac gtttgctgtc
    48541 tgatgtgaag ccgctggccg gggtcagatc cacatccagt gccgtggtga acatgtcgtt
    48601 gtgccatgat acgccggtcg ttacctgcgg acggatcctg aaggtttcag tgacaccatg
    48661 tacctctttg gtctcaatgc tgcgtgggat caggttctgt gcgaccagac ccagcgtcca
    48721 gttgtcatcc aggtccatgc tggcaccgat atcagcgtta ataccgtttt gggtgttgtg
    48781 gtaacggtca cctttgaatg cgctgctgtc atagtttttt atcagcgtat tgtagttaaa
    48841 caggtcaaca cgctggaatt ttggtgtgaa gcccagtgac catttttgtc cggccgtttc
    48901 cagttcagtg gccagcgcaa cgcccacatc ggtgatcagt gcggcacggg caaaagcctg
    48961 tgaggtcagt ctgtttttat ccacatcctg gctgccactg tctgcaatgc tttccagata
    49021 gttcaggtcg gcatcgctga ctttaccgtc gacgctgaca gtcccgaacg ttttcagcat
    49081 cagtgcagca gcgagggtat caccgggaag ggccgccaca gcagataccc cggcctgtgc
    49141 gctggcatgg gtgttccgga agtcctgcag tctctctttc aggcgggccg ctgtctgcgg
    49201 taccccggag tggctgtcga cggcacggtc gaaggctttc cagtcatcgc tgattttgtc
    49261 ggcgttgtcg gcgatattac ccggatcgga cagctgcgca cccacggacg gaagcagaag
    49321 actgaagtca tcttttgcac cggattttgt cagcagtgcc gggtttgcca gtggggccac
    49381 accatagtga gaggaggcaa cccccgttcc tcccatcgca tcattgcggg cttcaaaata
    49441 tgacgttgct gcagtcgaat taaaggaaaa taaaacggca gacgcgatga gagtacgggt
    49501 aatatgcttt ttcatcatca tttcagtttc ctgtattgag gtattttggg tataacaaca
    49561 gagggcacgg ctgtgccatt aaaaacagtt ggtgtattat tccggtttat tgctcagtat
    49621 tattaccagg gggataacgg aaccctgaat aacgcagtgg gtaatatttt tcctgttatc
    49681 agatattctg cctgtgccgg agtgagtgtt acggcgcgaa cagggatatc cggagatatt
    49741 tttcgggcca tctgaaacca gccttctttt tctatatcca ttctgaccgg atataacgaa
    49801 agtaccggat aaataatggt gttattaatt tcttttttct gcctgagcag aacgccgcaa
    49861 tattctccgg aatgcgttcc ggcaaacgga aaaccatgcc agagaataat gtccatattc
    49921 tcgcgggctg aaataatcct gcagaatatc tcctgtgtgg ctggccagtc tcccggacga
    49981 tacagtggat aatgcaccct gccgttcagc cattccggac tgagataaaa ccagtcagcc
    50041 agtgctgcaa gaatctcccc ggtcagatag tcagtgagtc tgtccggctc ccggaagacg
    50101 gcagggcgga tattccaggg ggccagtaat gcagccatcg ttgttgcgct gatatcatgt
    50161 gcctgcatga tgtgctccat cctgctgaga agacggcgga cgatgttatc tgcaggaaga
    50221 aacatttcac ttaacgcgtc ttcgaccagg atacgtgtca ggtcagagac ggaaagagaa
    50281 agctgttcgc tcatgatacc aagggcatcc ctgactccct gcggataacg aacctgcagg
    50341 ggtgtggact gcagctggcg atgactgagc aggcattcgc aaattcgctc cctgtccagg
    50401 gagagcatca tacgggcggc aatacctgtg ctgtcagttt tcatttcacc tccatgcggg
    50461 tcagcgtgtt ttgttcataa attgagtcta aagagagtgt tttttaaacg gaagcgagtt
    50521 atttcgatcg ttatgatcga gtgtgtttca agagtgtttg caactctgtt ttaagggcga
    50581 atgaaaagga ttcggacagg atgaccgtga ttaatttaac ccggaagcaa aagttgattt
    50641 tcattctgat atgccttcag tttgttaacg gacaaatcat gacccgttca tcaatgtttg
    50701 cgggtagctc gcctggcgtg ggaacgcgtt caccccgctg agccgcctgt gccagtgtgc
    50761 gcagcaacaa tacgctggca tgagatatag ccagttcccg ggtttctgca cgggtttcaa
    50821 ggtcgaaatc gtgcagtgtc acgacaaaca gcccgtttct gcacccgaca gttgcagggt
    50881 agggaacaaa ataaggggaa attttgctgg tctgtttacg cttggcatcg agtttgctga
    50941 ccagctgata tgcccggaga tgcgtgtacc gttttaacat gttcagggag cggtggcctg
    51001 aaatagcggc aacttcaatg acattcaatg taccaagttc gaaaaatcgg ctgatggctt
    51061 catgacggag atcgtgaaaa tgcagatttt ctattttcag gtccagaagt gcggtacgcc
    51121 aggcgctttt gaaaccggat gaggtgtaac tgaacacatt gccgtttatc tgttgtggca
    51181 gaatttgcag gtagttgcga gctttccggg agagtgggac atcgcgtggt aaaccatttt
    51241 tcgtttcagg aagatgcgcc actccgtgct gcaaatcgag gtgttcccag cgtaatgtga
    51301 ggatttcccc ctgtcgcatt gctgtttcca gggccagatg gaagataacg tagagctgct
    51361 gatttttatc tctgaaatag cgggagagtc ggcgttcttc gccagaagtc aggcgacgat
    51421 cgcgtccact actgattttg ggttttcgga ccagttcaac aggattcata cggcatgtcc
    51481 cccattccac actggcgata ttgaataatg atgataaaag agcaagttca agacggacgg
    51541 tgtttccggt gatttgacgc cctgttcgtg ggttaatttg agccaaacgc tgatcccggt
    51601 aagtggcgat atcaacggtg gtgatttcat ccatatagcg ctcggccata gggtgacgtt
    51661 taatcacatt gacgcgataa aactcctgca gatgaccttt tttgtggatg gatactgtct
    51721 tcaggtattt gtccagcgcc cggttgagag tcattttgcg aatttttatt tttcgaaaca
    51781 tatctgcctt ccacgataaa tccgaggaag gtaggaatac tgaatcagaa atgcaatgtc
    51841 tctgtgccaa tccggtcggt ggtctggtgg caataaagtg aactactccg cctgtaagtg
    51901 gtatcaatca tctgtagcaa tgaatcactt cattggcggg aaaagtggag gatctatcta
    51961 ctacaaaccc attcaatgcc cgaccggatt tatcatgaca ggtacacgca tgtatggcat
    52021 tggtgatggc gttgatgagg aacacgttga tgcttattgt tgccccttcg gttaaatccc
    52081 agcaccagga agattcaggc atgtaacagt aattgtgccg ccggactcat aatttcccca
    52141 cgcataatta ttcacatacc aaaccttgtt ttggtcggag tatacctgct gaaacccacc
    52201 aaaaggcgca ccattaaggt atgtccacga acagtacgca tgtacgccaa tgctcagatt
    52261 tcgggtgttt ttcccgatat tgtacgtact ggtagtgaag ttcacttttg ccgttgtcca
    52321 gataccggat tggcactggc ctccgtgcca atccggtacg tggaaaaaaa ttggcgcagg
    52381 tgatagccaa attgttaccg cttcagctac tgcgtggcgt tggcctggag caacggcaac
    52441 atgtccatct ggcaagaaag tcattggtgg aggcggtcaa tgcagatcaa ataccggatt
    52501 tatatggctc acgcgttcca tgccttcagg caacaatgca tggactgcca gttgcgacac
    52561 gaccgaagat caaaatggtt ccattacagt ttacgccatt tgccagtgac gatacttatc
    52621 actggcaaac cacgatgtaa ctacatttcc ccgacaggtc agttcggtga gtaccgccat
    52681 aaaagttgta ttcgtcatag aagccgcatt tcacaattcg attggcatca taggttgcaa
    52741 ctgaccccgc aaaataattc ggcttccacc cgtgcggatt ttctgcatta ctgacactcc
    52801 aggtacactg gctggcagaa aaaccagatg gcaatgggat ctgttgtcca tttgctattt
    52861 ttcctgtcag aacagtgcta cctgatgccc ttctccacgt accggattgg cacggaggcc
    52921 agtgccaatc cggtgtgtgg cgaacgattg gtgcacctga cggaagttac agcaatctgg
    52981 gttctcaccg gggaacattt aacgggagaa ataacggcag aggaactttg tttgtgtacg
    53041 cttccggtgg aaatggtgcc ggtggcggtg attgcgccaa cacatcaaat cttcagggct
    53101 atgtgaacgg cgctttcatt ggtatgaatg ccagcaataa cccctcctac ggaaagacag
    53161 catttatttc atttgccgtg ccagtgggag cgtcatatca aatcatctcc agaccaacac
    53221 aaaattacgc ctgtggtaac ggtgttttct ctgtgtatgc ctatcagatg tgagtcccta
    53281 gctggcttta acaatgtagt tgaatgcaat atttttggga cgcatttctg tggtactccc
    53341 ctgtgcccag tatggcatac cactatgatg ccctttcagc catccgccac gttcgtgata
    53401 gacgcctgat tgatcggcct gataactcag gagttgtctg ttccgatcac catcaagtcc
    53461 cttcccatta tcccagccac ggataaaaac accgcgcaga tcgggcaatt tacagctagg
    53521 ataagcacgg gcaagctgag gataccggga acaggaaaac gactggccgt tcatcaacag
    53581 atagccttgt ggaggagtgg cagatggcca cgggataggg cttcccacag ggtaggattc
    53641 aaagccaacc cacacaccgg attggcacga aagtattgcc cctttgctgt cacggcctac
    53701 aagtccatct ggtgaacact ttgtatttgc tacggctgtt ttatccagct gtaaatactc
    53761 acctgtggaa aggcgaccgt cagaccggac tgttccccca ctgacttttc ctcctttgat
    53821 ttcccctgag gttataattc ctttaccacc gactgcgcgt atccactggc tgtctgtcat
    53881 cgtaaaaccg ccaccatagg tgatgttctt ccagccttta tcatttttgg tgattaacca
    53941 gccgttttcg cttgtaacat cacctttcag tgttgcactt tgtccattga cgtttcctgc
    54001 attgttcagg ttgttccctc ccatattgat ggccgtgttc atctggttca gctccggtcg
    54061 tcccggtacc tgaaagcgat acaggcgatc gctttcctgc aggtcatcac ccatcattac
    54121 gatggcgatg tgcccgccat tgcagctgag tccatagtcg ccgggtttat ctgtccatcc
    54181 accaaaaaca ccggttgctg tgccattttt tcctataaat ccgcccagac cggggagcag
    54241 gctggcgata ctgcgcaggg catcgtcttt gatattctcc cctccggtgg agcaggtcag
    54301 ggcctccaga cgaccggtac tcgtgttacg acggatgccg gtggtataac tctgcccaaa
    54361 gggcgagcgg ttaagaccct tcagatatcc tttccggatt aactcatccg ccgtgaaggt
    54421 ggcaggaagc gttccgctat gcaggcgggc attgttatcg gcgatgtagg tcctggctgc
    54481 agtattgtag gcatctgcat gaaatgcggc gctctggttg cgcatttcat tcaggtagtc
    54541 ttcaatgtaa gaccagaaca gcggcatgag caggctgatg acaaccagtc ctcccagaat
    54601 ttcaagaagt gcaaaacctt tatcgttgtc tttttgcata gtcgttccct ccggtattac
    54661 caaaggccac cgggataaaa agtaaatgcg tactgtgacg cgcagagaaa cggcccaaag
    54721 ggaagttctc tggtaatgcg tcgttgtctg ttacaccgca gataaagtaa ggctccgcct
    54781 gctgccgttg cgacaatcca ggggatgtct cccggcgggg accagatggc aataccggca
    54841 atcagaaaaa catcgcccag gcctatggtt tctgttttcc tgcggtaaga ggtgtattgc
    54901 ctgaacagaa gcaacagcag gaaccagaac cctgcactgc acaggcctgt cagccagtct
    54961 ccctgccaga gacgaaatgc gctgccgctt atcagataca tgatggtgaa gggccgggga
    55021 agacggcagg atgcagcatc cgtcagggtc atcagtgtca tacatccggt cattatcgtc
    55081 ataaacagca ccccgacagc actgtcattc tgataaagac gcaggataat ggcggcagct
    55141 gcaagcatcc cgctcatcag cgcaagccca tggtgagggg ggctggcatt catccgggag
    55201 agtcgatagc gactcatctg aacggccaga cactgtatta ccacggttat tgcgaccagc
    55261 agtgccggga tcatctggag catggcagtt ctcccggact aacgtccctg catgtaagcg
    55321 ctgtaaatct gccgggcgta tttcatccgg cggtgagtgt tacttttact gaagccggcg
    55381 ttataactgc cgaggcattc ccagtttacg ccgcatcgct gaaaatgccg ggcgaggatc
    55441 catgctccgg tctgaatatt caggcagaca ttattcaaca gatctttctc actccggata
    55501 atgcccattt ttttcagccc gggaatatgt gtggagttaa tctgcattat cccgtaatcg
    55561 gtactgataa tttttcctct gtcattcctg ttatgattaa tggcccgggg attcagactg
    55621 ctttccctgc gggcaataat tttgagtaat cctggatcaa tggaataacg ttttccggcg
    55681 gtaataaaac aacgatcata ttgtttgtcc tgtggggatg cctgtgcgga aggaagtgag
    55741 cataacagtg aaaaaatcac tgtcaggata aataacctgt tcatacagtg accttaaata
    55801 aagcgcccag gcgggcgctg atgaatgatt aactgttggt ttccagtttg atagtagttt
    55861 tatctgcgtt acatactgta gacgggtcaa ctttattcgt cacattgccg ttgatttttg
    55921 taaacatgga tgaactgcgc aggctggtga ccagatcaat acagtttttc ttcggcacat
    55981 tgctggattc aataacgtag gagtatttat ctgaactgac tttgatggtt accgcaccac
    56041 cccaggcatt tttcgcctta ttaccaccgg cgatcatatc ggcaggcagg ccaccacttt
    56101 cggtcaggat tttgacgtag tcagtacctg tatactgccc ctggaatttc tgggctttca
    56161 ttttggtggc gattgtctgc aggtttttga gttccttctg gttgtctgaa gaggacaggg
    56221 tatcggacat aagtcccatc acggcaccaa taacaatggc agcgacgacc agaacaccga
    56281 ggacttccag cagggtcatg cctttatcat cgttctgaag gtgacgaata ttatgttctg
    56341 aatacgaaca ggttatgacg ctcatattag ctcctgagat tggtaaaaca gaatttggat
    56401 cgaggggaat ataaaggatg ctgtgtttac tgcttcagca tgtcactgat gccgtaaatg
    56461 gcactgacca tattgatgat gaagagaaat accagcagac ctgatatagc agtaataata
    56521 atgccgcttt ttctgacact cttttttgct tcttccagcc agcggtctgc ataaagcgcc
    56581 atactggcaa cactgtcctc cctttctgaa tttaaaagaa gattattcac acaggatttt
    56641 gacggaaagt cataaccact gtttttcatg gccagtgcca gtgactgacc ggataagaca
    56701 ttttttctga tagcacttat tcgtgatttt aaccatcggt tatgttctgc atgtttttct
    56761 attctggtaa gggcatgaga taaaggaata tttatctgca gcatggatga aatattaaaa
    56821 aggtaattca cgccatggaa tgtctgatac agggaccacg gcgggatgcg atcaagatac
    56881 cgtcgtcggg gacctttata cagactgaat gacatcctga tgagaattgc cagcagaagc
    56941 agtccagcca gcagccacaa accataaccg ataaccagct cggaggattt ggccagaatg
    57001 accatctgca ctgaccagcg ttcttccggt gcaaaggcgc tgatcatcgg aataacccgg
    57061 gtatgaacgt tatacatatt caccacacag agcgtcagca ggatgagcgg atagaggcag
    57121 acactcacca gactgctaat catctcctgt cgtccttttg ccgttttgac aacctgaaac
    57181 atggcttcag cgatatcacc gcgctcttca ccgttacgga tcagggcaat ttcatctttg
    57241 ggtacccagt ccttcagggc cggagagagc tgaccactgc tgcgtatgct ggcgatacag
    57301 tctgtcgcca gaaaatacag gccggactgc cctttctttt taccaaaatc cgtttccaca
    57361 tcgcagatga actccagtgc ccgcataacc ggtgtgccgt cccggatcat gcctgcaagg
    57421 gactcgtaaa acgtagtccg cctgaacgtc tttttgccca gccagcggtt aaggccggac
    57481 agttccagtt ccgtattgtt gagtctttcc agaattttca tatcagtgca gcagcgtata
    57541 gctgtcctcg tctacagggg atatgtaatg cgcagccagc ggatcgacct gaccactgtt
    57601 gattttgccc agcaggtgct gatttcgtgt cataccgccg agctcccggt gccagtaggt
    57661 ttttgcctca atccggcctt tttcccggta aatctggaaa aacttcgcat ccggggaaat
    57721 cacttctgca atgacggtcc ggccgttcac tccacgccag cagtgtgggc agccgttatg
    57781 gttacgaaga tacacggcat cgggctggca gaagttttcc accaggcgtc gttcttcatc
    57841 cgtccgggag ctctccacct catgccaggg gaggcggcag tggggacata tcacctgaac
    57901 cagacgctgg ctgagcaggc cgataaataa ctgcggatct gcaatcatcc ttgcctgaac
    57961 gccctccatt tccagacgct caagaatatt tatcgggtcg ttagcatgaa gggtggtcag
    58021 catcaggtga ccggtcatgg cggcttttat cgccgtgatg gcggaggcat ggtcacgtat
    58081 ctccccgttc aggatagcgt caggatcaag gcgcagggcg gacttaacag aatcgaccca
    58141 gccctgagcg gtatccataa cggccgtctg aattgcaccg gggatacgcc cctctggtgg
    58201 tgattctatt gtgaacagac gtcgccgggg gagcaggatc cctccggtat cgttgaagcc
    58261 atactgttcc agataggcgg cagaagccgt cctcagcgtg gtggattttc cggaaccggt
    58321 ggggccagag aggatcacga tgccctccgg tcgctgcagc atccttcgga cagcgcgctc
    58381 ctgctcaggg tgatacccca gcatactgaa tgtcggtata tgctgactgt catccttgat
    58441 cagacgcagt acggcataga gcccgcccac acacggatag tgggagtagc gcgcaccaaa
    58501 cagtccggcc agtttcagga atctttcagc cagacgggca tcctggggga caccgggatc
    58561 aaactgtgtt ccctttatga cgtcagtcat tccggaatag gtggccccca gtaattccag
    58621 cccctcatct tttctgacga tatcgagcag ctccagctct ccgtggacac gggcctccag
    58681 atacgtgaaa tccgtactgt cacgccccgg cgtgatgtga agatctgagg cgctgagggt
    58741 ggccgcatgg ctgatatacc cgagcatttt ttgctgtacg gggttaattc cgttttccac
    58801 cccggaaatg accgtctcct gccggagtcg ggcgatcaca tcaagtgtgg tgaactcaaa
    58861 cggggatact ttacccggat agcgggagca caggcgggtc agataatcct ggacggcacg
    58921 aaggtgtttt tttccttcca ccactctgac acgaatttgt cctgttttat ctgtatcaac
    58981 aaccacgtaa tccagaatgt catcaggtgg ctggtctgca ggtgccagca taaaatctcc
    59041 ttacgggcag cggtacccgg aaacgatttt tttgtgatta agggccgggg gaataatctg
    59101 ttccgacagc cgcctgtaaa tggcggcact gagtcagggt tgtagtgttg ttctgatacc
    59161 attttgttca aggacaacac tgtcagtggt gatcttttcc acgcggaatg tggtgccggg
    59221 aatgtgttgc ccttctttta catcagcagt tcgtccgccc ggcagttgta tcctggcggt
    59281 tatctggggc tgaactccgg atatctctgt cagccgggga ataccggtaa tgggggcagg
    59341 ggtatctgtc gtgacggctg aactgctttc ctgtgaatgc acaggctcag ctggttctcc
    59401 ctgctccagg gttctgagtt catgtcgcac cttttctctt gccagcatgg cttcatacag
    59461 tgcagtttct tcctgaatct gttccacctg gagttgccgg ttattctgtt gcatgttgct
    59521 gatggtgctg gccgcgaccg ttgtcggcag gatcaggacg gaaagcagaa gagcttttgt
    59581 tttactggac ataatattta cctgtgaatt gataacggaa acgaccctct tccagttgcc
    59641 ttgttacgga gataaaacgg acggagggtt cactgaaatg ctctgccacc tggtgcgccg
    59701 gaagtgtcgt cagtatctgc aggtcatact ccttccaggg gggctggatg gtatttccgt
    59761 cttcatcggt gaagctgttg ctgacttcct gccagtccat tttcagctgc atcgaccggg
    59821 cgagtgtggt cagccgttcc tgcaggaggg tgttttccgg taatgcctca acggcaacgg
    59881 gagtttcagg catactgaag gtgaccggca gggcaaaacc accgctattc ccgtccggca
    59941 taaccataaa cggccggatg ctgaaaattt ccctgacccg ctggctgaat gcggtcactg
    60001 tcgtcccggc gatgtttgta tatgatgcac gcagattgcc gctgttcccg gtggcagcac
    60061 actcggcgag tgaatatcgc cagccggcga ctgacaccgg caggcgtgac catacgtcac
    60121 tgcatccccg gataaaatct ccggtttccg gcatactcag ccacggtggt gtgatccgtt
    60181 cctctgcggc tttttgtgct gcttcagcaa gtgctgcttt ttctgccttc aggcgttcat
    60241 agtactgcca gccataccat gcagtaccca gaagggtgag tatgagtaat accggaataa
    60301 gctgtggccc acgggatacg gggtgaaggc gtgcctctgc cggatatttc cctgcacctg
    60361 tcagagtttc cagtggcagg ggacctctgc cgtcaggccc gttaaacgag ggcggttcat
    60421 aacatgtcca gccgtgttca ggcgcgctgt taatggtgag gaatatttcc cgggcctccc
    60481 tgatttcctc ccgggttccc accaggtcac tgaccaggat gccgtcaaca gcagaaacaa
    60541 aaccataaag ttctgtatcg tttgcctgtg acagttcaac gatagcgtat cctcctgact
    60601 cgagcagggg caggataagc atggcgagag agaaatacct gcggctgcct ttggggagtg
    60661 tattctggct gacggctgca accatgatgt gacgatcggt atcttccgta ctgagatact
    60721 cacaggaaat atagcgtact gcccccagac tgcgggcaaa cgtccggggg tttttatggc
    60781 ttgcgggcag tgttttccag tccattccgg caagaaatac attccgtcct gccgtcagcg
    60841 gaaacacctg gcgctctgtc ggtgaaatat ccgtcacgat agtcctcctt ctgcaaatca
    60901 gatgatattg ggcgtgatca gcaccacgag aatggtgtcg tcgttttcac tgttgatccc
    60961 accgccaaga ccgaagaatc ccggtgtgcc gacaccctgt tttccactgc gacgccctga
    61021 ctgctggaag ccactgataa tgactgtctg cccggatttc aggtccactg tctggttgat
    61081 ggttttcaga tccacattcg gcagctgggc tttgcttcca ccactctcgt aattctcaat
    61141 aaccggtttg tcggagagac tcatggaata gagcagctgc agtgtctgtg agtccgggag
    61201 aatgaacggc agaagggtca tattaaaacc ggtggtgatg gtggccgcat tcagggaggt
    61261 ggatgagccg acattggcgg tggtatccgt cgtcacactc tcaatgtagc tctgctgatt
    61321 ggcaatctgc atcggtaccg gggtcaggtt tttggtcacg gcggaattcc gggttaccac
    61381 actgacatct ccctgggaag agagtgcttt caggaaggct tttgaaccgg tgagtttccc
    61441 gttcacaatg gaaacaccac cagtgacggt atcgctggct gcactggtga atgtgctgcc
    61501 gaggctcagt cccagcgttc tgtcgctgaa gactgcgttc cagtcgatac ctgcctgctc
    61561 attacgggtt ttccggatac tcagaatttc cacattcagc accacctggc gcttcatttc
    61621 ttcattgcgt ttttcaatga acgtccggac cgtttccagt acctgtgggg tgtccgtgac
    61681 ggtgataaag cccgtcgaca tgaacatgcg accaatcccc ggcgtcagca ttgatgagac
    61741 agcatttttc acatcctgat acagcgcaga tttcatatcc acggtggtgg tctgggtatt
    61801 actggcatca ccggaaatgg catccgatgc tgttccacct gatgagccat ttgatgacat
    61861 ggtaccgctg accacggtgg cattataggc aacctggctg tccatatagg tcaccgggaa
    61921 ggtccgggta tcaagataat agaacgtgat atgtgaacca tcataacgcc aggaaatccc
    61981 gagacgggaa gcgaccgcat tcagcagtga tgtcagtcct gaagccttca gttcaggaag
    62041 tgacgccgta tttccctgca ggcggggaag tgtcgtgtca gaactgccta tggcggccag
    62101 tggaaccata ccgccggtat ccggcggtgg tactgtgccc tggatttgct gcgtactgcc
    62161 ggaggtggtc acgctcagat acgcctgtac atcaggggtg atccttacag gaatatgaca
    62221 gttttccgag atccgctggg ccacctgctg aagggtgatg ctctgccggg tgttaatgat
    62281 aatcggacag ccgggcaccg cattaccggg gtttttatcc gtgaccggac ggggattgat
    62341 ccactgacgt ggggattcct ttacaaccgg tgtccggttt cgcagctcag cgatcatttc
    62401 ccgggcccgt tgttgttcag caacggcgtc ttttctggtg ttctctgcct gtcggaaact
    62461 ggtacagccc tgcagcagag tggctgcaat cataatagca agaagttttt tgttcataga
    62521 taaatccgtg agttattcag agtccggcct aattcatcca gatgatgacc tgatcgggga
    62581 tcacagcagg gagcgtgatg gaaatacgcc gcccacgggt gtccaggagg cgaccgtttt
    62641 ccactctggc aagcagggcc gaatcaccac tttgttccag gagggcaccc ataaggccgg
    62701 gtttttcttt tgcccagaca aaaacgcgct gctgactgat gaggtggctg acgtttttcg
    62761 tggcgggcag accgagctgt gctgacgtga gctgaccacc ggcagcacgt cgttcagggt
    62821 gctgatacag gtaatcgttg atggcattca tataacgaat aaagtctgca gcctgctgtg
    62881 tgatttcagc ggtctgctga tggtgcacag tacggctgag gtgactgtgg aaccagaaat
    62941 taccaatggc cataatgacc ataatgaaac ccgtaaccca tcccataggt tattcctgcg
    63001 ggcggtctgt cacacggagc aggcattgtg cagtgctggt ccgggcatac agcggttttt
    63061 cagccggggt atagagtgca aagagctgct ccagcgcctg gcggaaactg ccggtgaaaa
    63121 ccagaggggc atcgatgctg tagttgacag gtgtttccca gcgcagtgtc catgtgccgt
    63181 gcgggcattt ttcggatgat gtccagcggg ccagtccgtc tttcagggtg gtcccctttt
    63241 ccattttcca ctgccgtggc ggtatgacct tctgctgtgg tggcactacc ggtttcgtgg
    63301 ccaccggggc tggcgtggtt gttctgctct ctggagttcc ggatgtcccg gtggtggtct
    63361 gagcccggga aacgctgacg gcaggtgtca ctggtgtcgg gcctgccggc gttgcggtgc
    63421 tgcccctgaa cggattacgc ggtgtatcag cagctttctg tgaaccctgc ggtgccgtga
    63481 cgtcgaccgg cggtgttgca gaggtcgtga cagtcagtat tttcctgttc cagtccatat
    63541 gtccgtaaag ctgctggccg gccagcagcg cattcagtgc ccggtgccac tggtcattaa
    63601 cggaccagac ggggaggcgt ttgttcagtg ccggtatggc agcgttctgt tgccggaact
    63661 gccagccggg tggtagcaac tggcgtatcc agtcactgac ggagcgctga tgtgcctcac
    63721 gtaaataaac gacggatgac tgaccgggtt taccggtcat aaccagtgcg ggcagctgtg
    63781 tggcctgaag gagcggacgg ggaggcagtg cggtactctg gctgctgact gcggttgtgg
    63841 gcgtcgctgt tgttgtcggg cggggcggcg tcacagggcg ggtcaccggt ggcggaagca
    63901 gacgtttttg cgtatgggcg ataatggcag cgctttcgcg aatttgtccg tcgacgaaca
    63961 ccagagccgg aggcgtggtg tcaggaacag gcgtggcatt aaatgccgta cagccgctga
    64021 gtaatgacgg cagaaggagt gtaagtgatt tctttttcat agaataaatc cggtatgtat
    64081 cgggttacgg tataagacag gtggctggta acaggtcttc agataaatat aatttcatca
    64141 ggatattgat gatgaacatt catgttcggg gtgattatga aaactaaaac attccggttc
    64201 cggaaatata aaaagaaaaa cgtcctgctg caaatgatag tttccagaaa acaagtgata
    64261 ttgtttttca gaactggaac attattttat ttcttgcctg ttattgaaac atgtccggtt
    64321 atgatctccg aatggcaggt atcaataaaa tcacttttcc ccgattgtgt gtcaaagtca
    64381 acgtgggtga atctgtgtac atcctgccat atggtgaaac agacaggaca ggtaacaggt
    64441 agtttacctt cagctatttt ttcgcagctt tcaaccagtt gctgcgcctt gttgtatctg
    64501 acgttatagc catttgtgca aagcgtttca tacccatgag ctcctgtaac tatatgtgtg
    64561 gcgattcttt cgtggactct cccattaatt aataattgtt cacgtgttgt gattgttctc
    64621 atgcctgttt ctccattggt cataacagaa aataatctgc attcctgaaa aatatcgcgg
    64681 ttgtgaaata tttaattttt accccccttt tatgggagat gagaagcagg ctgtcaggct
    64741 ataactgaac gcggtttctg gattttggtg ctaatgtcac atctttccat tcacagctgc
    64801 ttccggggat acgctctttg cgtcgtatta tacgggagtt ctgacacaca ataattaagc
    64861 cattgtctgc cgtctgaaat ggcattatag cgatgtttat atcttctgtt gcaggaatat
    64921 aactgacttt cttttcacat gtattggtgt atatgacaac tctgaatttt tctgcggaca
    64981 ttttcaatcc tcttttaaac cagaatatcc cgtgagggca ttctttgtga aaaagatgcc
    65041 ggaaaaattc cggcacgggg attaaacctg ttgtggttct gagttatccg gagcggttac
    65101 cggttcgctg acatccggaa ccttttccgg ggcaggcatg accagtggac gtgtcagggc
    65161 tgtaagtcct tcaccggata aacccacctc tttcagcagg gagtcgatga gcggagcctg
    65221 tgtacggtaa gccagagcgg cggttactgc ctgttcagcg agatttccgc cacttatgtc
    65281 ggttgttgct gttttaccgc gggtaacacc gccattcaga ccatccacct gaatgatttt
    65341 aataccgtca atggatttca ttggttctgt ggacttctct atgacggcag gtaatgcatc
    65401 cagcagggca agacggaagc ggaggctggt ctgctcatcc gaaagcacgt taatggcatc
    65461 gttcagtgcg cgctgtgctt ctgcttctgc cagacctttc tgacgggtgg cctccgccag
    65521 ttcaataatt gctgctgcct ggagttcggc tgcttctttt tcagcctgtg cgcgaaccgt
    65581 cagttcaacc gctttcgttt cagcttcctg agcagcagca atcagggcaa cctgtttggt
    65641 acggtcggcc tccgcagtct ggcgggtggt ttccacatcc tgctgggcct gtacggcctc
    65701 agccagtgct ttacttgccc gggcttctgc ctgtgactgc tcttccgatt ttgccgccac
    65761 ggcaatggct ctggcctggc tggcgatttc ggttgcctgc tgttgctcaa tttcccgtat
    65821 ggccacttcc cgttcggctt cgacctggcg ggtacgcact atctgctgtc gttcgatttc
    65881 ggcttcctct atcttacgtt cagccagaat tcgcgcggat tctgcttcac gacgtcgttc
    65941 agcttcaata gcagcaatac tggcactctg ctctgccgta cgggttttta cccgttgttg
    66001 ttgctccaga gtcatgaagg cttcctgctg ttcaatctcc agacgtcggg agagtgcatc
    66061 ccggtttttt tccctgatgg ccacttcaac atcctgctca acttcgttgc gttcgcggcg
    66121 acgacgttct gtttcctggg tcagaagggt caggccttca gcatcaaagg cattgtcagg
    66181 attaaaatgt acccgtgcag tctggttgaa acttgtcagc gacacacttt caagctccag
    66241 accgtttttg gataagtctt ccgcaacggt gttctggaca ccctgaacaa agttctcgcg
    66301 ggcatcctgt aaatcctgca tgctcattct ggccgccgtg gcccgtaggg catcaacaaa
    66361 tttatcctcg accagactac gcagatcttc aggtgtgagc gtgcgttgtc ccagcgtctg
    66421 ggctgcagtg gatattcctt cggctgatgg ttttacccga agaaaaaagg caacggcaac
    66481 atcaacatca acacgcatgc ggtcgcgggt tatgagactt tcagccgctg cacgactgac
    66541 ttccagcttc agggtgttca tgttgacggg aattgtttcg tgaaaaatgg gcataacaat
    66601 tgcaccgcca ctcattacca ctttttggcc tcccagacct gtgcggacaa aggcctgctc
    66661 tgccgatgcc cggcggtaca ggcgggcaaa tatcagcccg ataacgaaca gggtgaggac
    66721 cagcgctata acggtaaaca accatcccgg aagattaaac atagtgagaa cctctgtata
    66781 tgtcgggatt aaagaacatc cggccaggga ttcagttcgc ccagccagcg ggaaccggaa
    66841 agacggcaga caataatcac tttgtctcct cgtctgaatt ctttcccggc ctccggctcc
    66901 agcaacaggt aatgaacctg cccgaagcgg tctgtgaagc gaccttcaca gggggaaccc
    66961 gccactgcgt gataaccagt gatggtggcc atacagccga tgaatgcctc ctcggtgagc
    67021 gccgttgttt catcacgggg taaccagggg gcaatcagtc tgccggtgcg gtgtacgaga
    67081 ggacatgtca gcagcaggca aagtggtgca aggagaatgt tcggagcagg agcctgcagc
    67141 agatgaataa taagaccctg caggagaatg ccggatatac caaaaaaacc tgccagcata
    67201 cagaggacga tcagcagggg gatccgtccg atgttcagcc agtccagcgc ctctcctgtc
    67261 agaccctccg gtaaatcagg tgtatccata gctgaagaga gggagtgtcc gcagagcagg
    67321 gcgataagtt cagcgatacc agtaagcaga acaaaagtca gggctgccag atagggtgcg
    67381 ttatacgcgg taagtaacgt cagtgtcatt tttcctcctg tggcaggtca gtcaaacaac
    67441 attatggcgt gaagcttccg cttctttaat tgtcagaaga agcatatcgt cagtcatcag
    67501 aagacaaggg gatgtctgca ggaggggatg ataacaacag aagcctcacg ccggaatgcg
    67561 ctttatgaca accggagagg acggataatc aaaaatggga catccacatc agcaaaccgc
    67621 gatttgcgaa aaaaagtcag cggagagtgg ctgccgttgt ggctgtcccg gtatttcagt
    67681 gtaaaagcag actgttgtcc atgccccatt cctcttccgc taaacctgaa tggcccacat
    67741 gaagaatgcg tgacagcata tttctgccgc tgccgttcag actgcattcg cgcccgtgtg
    67801 cgagcataaa accttcatcc cggaatatct cttcaacctc agacgtgttg atggtggccg
    67861 gatggattaa tgaaaatgcc accggtctga tcattctgcc cgtggtggaa aaggtgtccc
    67921 cggtgctgtt cagctgcagg atatgacgtt tcccggtctg tgcgcgggaa agtgtctcac
    67981 tcagtaactg gtcattccat gcgcacgtgc caatctgcca tcccagactc ctgcacgtgg
    68041 caagagccga gcggaagcgg tacaggtcgt ataatgctgc gttattgcca ctttccagca
    68101 gtgatttcag ggtgtttgct accggtatgt ctgaattggt ggccagcagt tttcgcaggg
    68161 tagtgaactc ttcagccatt cctctgaact cgcgcatctg acgggcagtg tcaagccagt
    68221 tcagcaggta acgccggcga cgccgttcgt tgtttatgtt gtccggggcg gtggagagtg
    68281 cttcgagcat attctccagt aacagaatga cagggttttc ctgggataga atggtcataa
    68341 aggcgtcctg aaaaaatgcc cgacagccgc tctccgtaca ccctcaggtg tcaccggtgc
    68401 cgggcattgt ggaaacagaa gggaaaaaaa tcccggccat aaaggccggg aaaagtcgtc
    68461 aaattaagac agcaattttc atgtcaacgc tccgtcatca gataccaggt gcaggagcag
    68521 ggcgatcctc gactccggca gaggctgtgc atcaagccgg aggaggtact ggtaacgcgc
    68581 ttttgtcagc cagagggtgg aggcagcatc cagctcctcc ctggctgccg gattgagcgc
    68641 aggatccggg tacactttca tcaaagactg aacgatatcc tccctgacgg ttttgatttc
    68701 accggccatt ttcacgaagt ccagaaaggc actgtgacgg cgaaggtgag tgaagctgtg
    68761 gctttcgggg tctgctttca ggaaaaagta accgggaaac acggcgtgta tatgtcgacg
    68821 gtaaccacag actttatccg tgcggcggac ctttttcagt atcaggggtg tccagggaag
    68881 gacgtgttgt tcggaaagcc aggaaaagag atgttcgcgg tttttgccgg cagggatgta
    68941 ctgtgccaga taccagttgc gttgatgtaa ttcactgata ttcaaaatcc ggaacgctcc
    69001 gcgaaaatcg atccccgcag gggggcttcg atgttgttgt gaacggcgaa tacataaagc
    69061 atgtgtcgga aacagcggct gacccacgtt cagcctggcc tgcagttctg acaggcacat
    69121 gatttacgca ctcttgtaag ccttgtggac ggtttcgagg cgggcgaaaa ccgacaactc
    69181 ctgcaaacag gattaatggc tggaaaatat attacataag gatgtatctg tggcaagagc
    69241 gaagataagc agttgaatag atcgttatat ttgataagac agaatattat tctatatttg
    69301 atgttgtgtg cgggatatta gatcgtttaa aaaaattcgg cgatctcttc tactggaaaa
    69361 gaagaaaaaa gcagcataaa agatcgtttg tgaaattgtt catttttttg acagagttca
    69421 aaaaaacgac gctttttagg gggttaaaga gggcaactgt aactctgaga aatcgtgtcg
    69481 ccttttcaaa gcggtggaaa aggggtatat tgcggatcgt tattcagtgg cttttgggat
    69541 cctcgcggtc cggaaagcca gaaaacggca gaatgcgcca taaggcattc aggacgtatg
    69601 gcagaaacga cggcagtttg ccggtgccgg aaggctgaaa aaagtttcag aagaccataa
    69661 aggcgaaacc cccatcatct ttcctcgaaa cttggcggaa tcagagaaag ataatggggg
    69721 tatcacggaa tacgggacaa gtatatatga aaccgtgtca gagattcaac ccttcgcagt
    69781 gcattaacgc acggcaatct gttcccgcca taagcgacag cttgtggcgg ccctgaacca
    69841 gacctcattc actgaaagca cataccgtca ggtaaagaac ccgaatccgg tgttcacacc
    69901 ccgtgaagat gccggaacgc tgaagttctg cgaaaaactg atggaaaagg ccgtgggctt
    69961 cacctcccgt tttgattttg ccgcgcatgt ggcgtatgcc cgttcgcgcg gcctgcgccg
    70021 gcgtatgccg ccggtgctgc gccgccgggc gattgatgcg ctgctgcagg ggctgtgttt
    70081 ccactatgac ccgctggcca accgcgtgca gtgctccatc accacgctgg ccattgagtg
    70141 cggactggcg acggagtctg aggccgggaa actctccatc acccgtgcca cccgtgccct
    70201 gaagttcctg tctgagctgg ggctgataac ctaccagacg gaatacgacc cgacaattgg
    70261 ctgtaacatc ccgaccgaca tcacgttcac gccggcactg tttgactcac tcgatatctc
    70321 agaagaggca gtggcttccg cacgccgcag ccgcgtggaa tgggaaaaca ggctgcgcaa
    70381 aaagcagggg atggatgcgc tgggtatgga tgaactgata gcgagggcct ggcgttttgt
    70441 gcgcgagcgt ttccgcagtt atcaggcaga gcttaagtcc cacggcatga aacgtgcccg
    70501 tgcgcgccgc gatgcaggca ggacccgtca ggacattgtc accctggtga aacggcagct
    70561 gacacgtgaa atcgcggaag ggcgtttccg cggaagcctg gaggccgtga agcgcgaaat
    70621 cgaccgtcgc gtgaaggaac gaatgataat gtcgcggaac aacaactaca cacggctggc
    70681 cactgcctca ccctgagttt gacctcatct gaataatccg gcctgcgccg gaggcttccg
    70741 cacgtctgaa gcccggcgac gcacaaaaaa gcagcacagc acacaaaaaa cagcctcatc
    70801 atctgccttc cggctcatca tgctccccct gtttttcacg caaaacacgt ctcacagacg
    70861 ggtaattctg tttatccaca tttaactgca agggacttct ccataaggtt acaaccgtta
    70921 atgctataaa gcgccagccg caggcgttac agggtgcaat gtttctttta aactccagta
    70981 ctgtatttcc tttaaactac ttaattactt ccatttaaaa agaaaacctg ttcaatgcct
    71041 gtcctgtgga cagactgata tgcacctccc acgctgacgc gcgggccccg aacctcaccg
    71101 ttctgaaacc acagcaaaaa gacatcagga ataaaaacac cacacaaacg cagcaccgtg
    71161 cccacccctc ataactgaaa agcgaggccg cccccgccca aagggcggga acaacatcgc
    71221 ttttaattat gaatgttgta actaataatc ctcatcgctg tcagtcttct ggctggaagt
    71281 cctcagtaca cgctcgtaag cggccctgac ggcccgctaa cgcggagata cgccccgact
    71341 tcgggtaaac cctcgtcggg accactccga ccgcgcacag aagctctgtc atggctgaaa
    71401 acgggtatgg cttagcaggg ctggggatgg gtaaggtgaa atctatcaat cagtaccggc
    71461 tgacgccggg cttcggcggt tttactccgg tatcacatgt aaccacagcg tgccgccttc
    71521 catgccgctg gcgcggcata tcctggtgac gatatctgaa tcgttatata ctgtgtatat
    71581 acgtagtaat gacgaggtga taaatggcac aggttaatat gagtttaaga atcgacgctg
    71641 aactgaagga tgcttttatg gccgctgcaa aaagcatgga ccgtaatggc tctcagttaa
    71701 ttcgggattt tatgcgccag accgttgaac ggcagcataa tacctggttc cgtgaccagg
    71761 ttgcggcagg acgtcagcaa ctcgagcgcg gcgatgtgct tccccatgac atggtcgaat
    71821 cttctgccgc tgcatggcgg gatgaaatga gcaggaaggt ttccgataaa tgatggagat
    71881 attctggacc atgctggcca gccaggacag gaagcgcatt cgtgagtaca ttgcggagca
    71941 gaacctgatg gccgccattg aactggacga acggattggt tattcagcca gcagcctggc
    72001 cgggcagccg tataaaggtc gcaacggtcg tgtggaagga acccgggaac tggttattca
    72061 cccccatttt gttctggttt atgaggtcga cagccagtgg ggaaaagtgt atgtcctgcg
    72121 cgtgttgcat accgcacaga agtggccata aaattccagt caggaataat acttttcatt
    72181 tttagtttac tgctccccca gtaagagcga tatattccga acagtcttgt ctgggaggta
    72241 tggtgcagtc agcgaagaac cgccgagaat cctcgcccaa tgcgggcggg gagcagtcac
    72301 cggagtcttc gccacccgcc ctctgtgcac tgaccgggtg atcctgtcag tgagcgtggt
    72361 tctgccactt catccgaatt ctttcttcca gtttgctatg caatccgcct ccgatccact
    72421 ggctgaccag agctgctgtt gcaaatgaac agaaagtcaa ggcgaatcca tgtttaaacg
    72481 tctccggtag ttcctggccg gcgtgctgag tgatgatgga aaaccctata tatataataa
    72541 aggtacaaaa aattcctgtg tgggcgagcc aggcaaacca tttggccacc ctgtataacc
    72601 cgtatttcaa tggcatctcc cgtttaatat catgaataat cattgtatct atcttgtggg
    72661 tcagagattg aaattttgta aatatcataa gtccttagtc catctaaagt gagatttacc
    72721 gtatctgaat atatcaaaat gctaaggcag aatccccgga aatttgtgta ttacttctgt
    72781 agcagacacg gcaaaatcgt tgcattacag gtgcatctgc tggggcgtct gtttatcgat
    72841 gcatcagatt taatgtctga cagttacttt tctgtatcac ccacactctc tgctttttca
    72901 ttgctactgt catatttatg tctgttgcgc ggttttatct gtgtatataa aatacatctg
    72961 tattcattgt tttctaaaaa tttgcgtatg ttgataaaaa atccgatttc ttatttccat
    73021 attggcacgt ttttattttc aggaaaaaaa cgtcatcttc tggaatgctg gaaagcagaa
    73081 gttatcatgc gggataagtt tgtttttcac cggcttatca ggaacggtat gcagaggcag
    73141 agaggttttc ttctgtggtg gcgcctggct aatgaaatgt atatatcggg gaataaaaaa
    73201 caacgaaaat gtgcgatcaa gataaaaaat gcgcttatgg cgaagtatgg atgtgatatt
    73261 ggtctggggg cgcagattgg taaaggcctc gtgctgcccc accattcagg cgtcgtcatt
    73321 cacggaaatg taaaaatcgg tgagaatgtg attatacggc agaatacgac cattggcgaa
    73381 aaggaatctg acagcagaga gaattatatc gttattggtg ataatgtcga tatcggtgcc
    73441 catacgtgta ttatcgggct taacgtaaaa ataggcagta atgtaaaaat tggagccatg
    73501 tcttttatta tggaggaggt tccggataac tgtacttacg ttaccaggaa agaatcacgg
    73561 gtaattatgc gttaatcatg atgatattat tcacagtttt attgtattgt tgattgagaa
    73621 atttatacgt gcggggggga tggcctgagt acaggaagga agcccccgtg attgtggtga
    73681 tttttttgtt actttatttc agtatgcggc agatatccat tgcatccttg cggtcgcttt
    73741 tttcctttca aaaggcagtt acgtcgcttc tgccttacat ctgatgagcc tttggtggca
    73801 tccatgagtc tgtaagcaga ctctgataca caatgtcatg atgttatctg aaatgtttat
    73861 tgctcagtct gccgttcctt catcctgagc caccgttgtt ctattatcgc cagtctttga
    73921 acagcactct gtgcttcagt ttctgagacc tgcccatcgg gatgaccatc cagcccataa
    73981 cgagggacgc cggcgacaga ggtccgctgc agatatacgc ggcgggaggt gatgcaggac
    74041 actgcacaga gccattcgtc catggacagt tctgtatccg ggctttctgc cagaaatgcc
    74101 gaaatatcat tgcttatgcc aattttcagg ggaataaatc caccaccttc agcacgaaga
    74161 tgaggaaaca gggcctttat tttttccacg cactcgtgag tatactcacg ggtccagtgg
    74221 gagggacgtg gaaaacggtg tcttttcttt ttcttcttcc tggctggcgc ttcggggata
    74281 tgtgtttcga cggcagatga atttattccg gcgggggctg attctgccgt ctgttcagta
    74341 aatacaggtg cgataatatc tgttttttcg gataaggacg gtggcgaaaa agttcgacgt
    74401 tttttcacca caatgacggg agttttcctt tcttcctgat tttttcccat aaatacctcg
    74461 gctggctggt tttactcttt aataatatca caatccataa attagtaaat ttcccatcag
    74521 gtgatttttc atggctaata agtccattct ctgaatattt atactcgagg tagttacagt
    74581 tatggcatga cagtcgaatt gatattctac tgaaccctga cagaaacatg gttgatatat
    74641 tctctcactt taatgttgtc atgatataaa taaaaacaat tcatttgaaa ggatatcaga
    74701 agaagatatc ggggcgcaca gttcatctct gcctttgttt catggggtgt tctttgtata
    74761 ttttacttac tgtttaagca tgtacataag gagtgctttc attatgccaa tgatacttgt
    74821 aaagaagaat ccccggggca aagtgattcg ggagcttagc agtgaggaag agactgcagt
    74881 aaagacggtc tgtggactga aaaagccagc aaccatggca ctacataacc tggccaatga
    74941 tattctccgg gaaatgcgtg agtatgatgc atggttacag tgtgactgta ttccgggaga
    75001 ttcgccggcc atgaattttg ctgctcttaa aaacaatacg ggtactcttt atctgtccag
    75061 ttttaatcat gagcatgcac ctgaatgccc catgtatcgt cagttatccg gaaatgaaga
    75121 ggaaacatct tccggagcaa gccggcatcc cgttagcacc cgtataaatt acagaaactt
    75181 tttaccacct gatgacagta attctgttat cagactgcag gcccgctctg cttaccataa
    75241 tggtgagagc tcctccgttc gcaagaaacg tccgcgcctt gcccgtttac tcctgtcgct
    75301 tattgacgat gctgggctga ataaactgga ttccctggct aatccccgta tccggactaa
    75361 ttatcgtgag tgtcttgatg ccatacggca ggttacactg cagcaggagt atattcgggg
    75421 aagatcctta tctgaaatta tccatttccg gcctggaatg tctgaaaggt ctcaggagcg
    75481 gttaatggaa acgctggaga attcagaacg acactggcct gccagaagaa aacatatgtt
    75541 ctttcagatt tttatggcgc agcacatctg tcgtgatgct gtggaaattc actgggcgaa
    75601 cggtaatatt caggtcatca gacctgtgcg ggggatcagt attaatggtg aagcgcaggg
    75661 cggaatacgt cccccttact gggttattct tgctttttgc cggagtgccg atggcagaat
    75721 catctgcagt gaagggtatg ctcatgctct ttatcaactg acatgtccgg tgcctgtgga
    75781 cagcaaactg gaacgaaaca cgctcactgc tcttctgaat gtggccagct ggcttaaaag
    75841 aaagccaggt acgccggaat taagtctgga aaggcccctg tttgatacag aagtttatgt
    75901 taatggtgaa aagaaatatg tcctgccgga tttcattgtc acagcaaggg ctcctgacgg
    75961 aaagacggcc agagtggtca tcgaaacgat gggatatgaa gacagtgatt actgcgcgag
    76021 aaaatccagg cagcataccg gcatgaagca gattggtgtt ctgcataccg atccaccgaa
    76081 atggctggat aacgatcatc ccccttttga gaaacatatg tacggtgttt ttatgcatct
    76141 caggtactga gatattttgt ggctcagttc tgtaactttt cccgtaacat tgtctgttgt
    76201 tacgggaaag tccggttttt gtattgcacc agagaatact cagactgtga tgctgccaca
    76261 gcgtcagcag gctttctgaa cggtgtaaca ttacttcatg ttaatgataa tcactatcat
    76321 taagtcttga catgccattt tctccttaat aaattaataa tgtatatgta tccatatacg
    76381 taagcagtta attcatttgt tttcctcaga ggatgaagga gataccgaat gtctgaccct
    76441 gtacggggtt cagtgagtta gctggcctgg atgtaaccac tcgttcaact caattctctt
    76501 agcgtgggaa aatttccctt ttccaagcgg acccctatta atatttgttt tgttttaggt
    76561 tctattcaac ataggtcatc ctaaaataca tt
//
LOCUS       DAZOZX010000002        70711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZOZX010000002 DAZOZX010000000
VERSION     DAZOZX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.002"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..795
                     /locus_tag="ACHUPT_000084"
     CDS             <1..795
                     /locus_tag="ACHUPT_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0817842.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHUPT_000085"
     CDS             806..1897
                     /locus_tag="ACHUPT_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0817843.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUPT_000086"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUPT_000086"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0817844.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUPT_000087"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUPT_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0817845.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHUPT_000088"
     CDS             4859..5254
                     /locus_tag="ACHUPT_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0817846.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUPT_000089"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUPT_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0817847.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUPT_000090"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUPT_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0817848.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHUPT_000091"
     CDS             complement(6563..7603)
                     /locus_tag="ACHUPT_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0817849.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHUPT_000092"
     CDS             complement(7676..8311)
                     /locus_tag="ACHUPT_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0817850.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUPT_000093"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUPT_000093"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0817851.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHUPT_000094"
     CDS             complement(8937..9380)
                     /locus_tag="ACHUPT_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0817852.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUPT_000095"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUPT_000095"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0817853.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHUPT_000096"
     CDS             complement(9720..10223)
                     /locus_tag="ACHUPT_000096"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0817854.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHUPT_000097"
     CDS             complement(10217..10741)
                     /locus_tag="ACHUPT_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0817855.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHUPT_000098"
     CDS             10950..11945
                     /locus_tag="ACHUPT_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0817856.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHUPT_000099"
     CDS             11954..12832
                     /locus_tag="ACHUPT_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0817857.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHUPT_000100"
     CDS             12913..13920
                     /locus_tag="ACHUPT_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0817858.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUPT_000101"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUPT_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0817859.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUPT_000102"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUPT_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0817860.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUPT_000103"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUPT_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0817861.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUPT_000104"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUPT_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0817862.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUPT_000105"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUPT_000105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0817863.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUPT_000106"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUPT_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0817864.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUPT_000107"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUPT_000107"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0817865.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUPT_000108"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUPT_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0817866.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUPT_000109"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUPT_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0817867.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUPT_000110"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUPT_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0817868.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUPT_000111"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUPT_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0817869.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHUPT_000112"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHUPT_000112"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHUPT_000113"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHUPT_000113"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0817870.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHUPT_000114"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHUPT_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHUPT_000115"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHUPT_000115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUPT_000116"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUPT_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0817871.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUPT_000117"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUPT_000117"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0817872.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUPT_000118"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUPT_000118"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0817873.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUPT_000119"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUPT_000119"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0817874.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUPT_000120"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUPT_000120"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0817875.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUPT_000121"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUPT_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0817876.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUPT_000122"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUPT_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0817877.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUPT_000123"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUPT_000123"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0817878.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHUPT_000124"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHUPT_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUPT_000125"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUPT_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0817879.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHUPT_000126"
     CDS             complement(41212..42177)
                     /locus_tag="ACHUPT_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0817880.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUPT_000127"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUPT_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0817881.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUPT_000128"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUPT_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0817882.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUPT_000129"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUPT_000129"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0817883.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUPT_000130"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUPT_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0817884.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUPT_000131"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUPT_000131"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0817885.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUPT_000132"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUPT_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0817886.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUPT_000133"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUPT_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0817887.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUPT_000134"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUPT_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0817888.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUPT_000135"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUPT_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0817889.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUPT_000136"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUPT_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0817890.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUPT_000137"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUPT_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0817891.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHUPT_000138"
     CDS             49715..50680
                     /locus_tag="ACHUPT_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0817892.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUPT_000139"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUPT_000139"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0817893.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUPT_000140"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUPT_000140"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0817894.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUPT_000141"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUPT_000141"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0817895.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUPT_000142"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUPT_000142"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0817896.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUPT_000143"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUPT_000143"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0817897.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUPT_000144"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUPT_000144"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0817898.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUPT_000145"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUPT_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0817899.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUPT_000146"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUPT_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0817900.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUPT_000147"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUPT_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0817901.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHUPT_000148"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHUPT_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0817902.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUPT_000149"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUPT_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0817903.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUPT_000150"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUPT_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUPT_000151"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUPT_000151"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0817904.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUPT_000152"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUPT_000152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0817905.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHUPT_000153"
     CDS             complement(62547..63476)
                     /locus_tag="ACHUPT_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0817906.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUPT_000154"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUPT_000154"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0817907.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUPT_000155"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUPT_000155"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0817908.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACHUPT_000156"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACHUPT_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17317 a        19176 c        17557 g        16661 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaagttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctacagatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagcag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaagt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa g
//
LOCUS       DAZOZX010000003        67005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZOZX010000003 DAZOZX010000000
VERSION     DAZOZX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.003"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..601
                     /locus_tag="ACHUPT_000157"
                     /pseudo
     CDS             1..601
                     /locus_tag="ACHUPT_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            704..1027
                     /locus_tag="ACHUPT_000158"
     CDS             704..1027
                     /locus_tag="ACHUPT_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR0817909.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1094..2551)
                     /gene="nuoN"
                     /locus_tag="ACHUPT_000159"
     CDS             complement(1094..2551)
                     /gene="nuoN"
                     /locus_tag="ACHUPT_000159"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0817910.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2558..4087)
                     /gene="nuoM"
                     /locus_tag="ACHUPT_000160"
     CDS             complement(2558..4087)
                     /gene="nuoM"
                     /locus_tag="ACHUPT_000160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0817911.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4251..6092)
                     /gene="nuoL"
                     /locus_tag="ACHUPT_000161"
     CDS             complement(4251..6092)
                     /gene="nuoL"
                     /locus_tag="ACHUPT_000161"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0817912.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6089..6391)
                     /gene="nuoK"
                     /locus_tag="ACHUPT_000162"
     CDS             complement(6089..6391)
                     /gene="nuoK"
                     /locus_tag="ACHUPT_000162"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0817913.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6388..6942)
                     /gene="nuoJ"
                     /locus_tag="ACHUPT_000163"
     CDS             complement(6388..6942)
                     /gene="nuoJ"
                     /locus_tag="ACHUPT_000163"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0817914.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6954..7496)
                     /gene="nuoI"
                     /locus_tag="ACHUPT_000164"
     CDS             complement(6954..7496)
                     /gene="nuoI"
                     /locus_tag="ACHUPT_000164"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0817915.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7511..8488)
                     /gene="nuoH"
                     /locus_tag="ACHUPT_000165"
     CDS             complement(7511..8488)
                     /gene="nuoH"
                     /locus_tag="ACHUPT_000165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0817916.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8485..11211)
                     /gene="nuoG"
                     /locus_tag="ACHUPT_000166"
     CDS             complement(8485..11211)
                     /gene="nuoG"
                     /locus_tag="ACHUPT_000166"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0817917.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11264..12601)
                     /gene="nuoF"
                     /locus_tag="ACHUPT_000167"
     CDS             complement(11264..12601)
                     /gene="nuoF"
                     /locus_tag="ACHUPT_000167"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0817918.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12598..13098)
                     /gene="nuoE"
                     /locus_tag="ACHUPT_000168"
     CDS             complement(12598..13098)
                     /gene="nuoE"
                     /locus_tag="ACHUPT_000168"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0817919.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13101..14903)
                     /gene="nuoC"
                     /locus_tag="ACHUPT_000169"
     CDS             complement(13101..14903)
                     /gene="nuoC"
                     /locus_tag="ACHUPT_000169"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0817920.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14997..15659)
                     /gene="nuoB"
                     /locus_tag="ACHUPT_000170"
     CDS             complement(14997..15659)
                     /gene="nuoB"
                     /locus_tag="ACHUPT_000170"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0817921.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15675..16118)
                     /gene="nuoA"
                     /locus_tag="ACHUPT_000171"
     CDS             complement(15675..16118)
                     /gene="nuoA"
                     /locus_tag="ACHUPT_000171"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0817922.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16749..17687)
                     /gene="lrhA"
                     /locus_tag="ACHUPT_000172"
     CDS             complement(16749..17687)
                     /gene="lrhA"
                     /locus_tag="ACHUPT_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0817923.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18601..19818
                     /gene="alaA"
                     /locus_tag="ACHUPT_000173"
     CDS             18601..19818
                     /gene="alaA"
                     /locus_tag="ACHUPT_000173"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0817924.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19902..20501
                     /gene="yfbR"
                     /locus_tag="ACHUPT_000174"
     CDS             19902..20501
                     /gene="yfbR"
                     /locus_tag="ACHUPT_000174"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0817925.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20560..22392)
                     /locus_tag="ACHUPT_000175"
     CDS             complement(20560..22392)
                     /locus_tag="ACHUPT_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0817926.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22479..23129)
                     /gene="hxpA"
                     /locus_tag="ACHUPT_000176"
     CDS             complement(22479..23129)
                     /gene="hxpA"
                     /locus_tag="ACHUPT_000176"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR0817927.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23140..23634)
                     /locus_tag="ACHUPT_000177"
     CDS             complement(23140..23634)
                     /locus_tag="ACHUPT_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0817928.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23717..24172)
                     /gene="yfbV"
                     /locus_tag="ACHUPT_000178"
     CDS             complement(23717..24172)
                     /gene="yfbV"
                     /locus_tag="ACHUPT_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0817929.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24510..25712
                     /gene="ackA"
                     /locus_tag="ACHUPT_000179"
     CDS             24510..25712
                     /gene="ackA"
                     /locus_tag="ACHUPT_000179"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0817930.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25787..27931
                     /gene="pta"
                     /locus_tag="ACHUPT_000180"
     CDS             25787..27931
                     /gene="pta"
                     /locus_tag="ACHUPT_000180"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0817931.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28121..29641
                     /gene="yfcC"
                     /locus_tag="ACHUPT_000181"
     CDS             28121..29641
                     /gene="yfcC"
                     /locus_tag="ACHUPT_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0817932.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29674..30216)
                     /gene="yfcD"
                     /locus_tag="ACHUPT_000182"
     CDS             complement(29674..30216)
                     /gene="yfcD"
                     /locus_tag="ACHUPT_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0817933.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30274..30825)
                     /gene="yfcE"
                     /locus_tag="ACHUPT_000183"
     CDS             complement(30274..30825)
                     /gene="yfcE"
                     /locus_tag="ACHUPT_000183"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0817934.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30881..31525)
                     /gene="yfcF"
                     /locus_tag="ACHUPT_000184"
     CDS             complement(30881..31525)
                     /gene="yfcF"
                     /locus_tag="ACHUPT_000184"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0817935.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31661..32308
                     /gene="yfcG"
                     /locus_tag="ACHUPT_000185"
     CDS             31661..32308
                     /gene="yfcG"
                     /locus_tag="ACHUPT_000185"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0817936.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32365..32727
                     /gene="folX"
                     /locus_tag="ACHUPT_000186"
     CDS             32365..32727
                     /gene="folX"
                     /locus_tag="ACHUPT_000186"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0817937.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32748..33641
                     /locus_tag="ACHUPT_000187"
     CDS             32748..33641
                     /locus_tag="ACHUPT_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0817938.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33689..34579)
                     /gene="rpnB"
                     /locus_tag="ACHUPT_000188"
     CDS             complement(33689..34579)
                     /gene="rpnB"
                     /locus_tag="ACHUPT_000188"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0817939.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34776..35549)
                     /gene="hisP"
                     /locus_tag="ACHUPT_000189"
     CDS             complement(34776..35549)
                     /gene="hisP"
                     /locus_tag="ACHUPT_000189"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0817940.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35557..36273)
                     /locus_tag="ACHUPT_000190"
     CDS             complement(35557..36273)
                     /locus_tag="ACHUPT_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0817941.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36270..36956)
                     /gene="hisQ"
                     /locus_tag="ACHUPT_000191"
     CDS             complement(36270..36956)
                     /gene="hisQ"
                     /locus_tag="ACHUPT_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0817942.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37046..37828)
                     /gene="hisJ"
                     /locus_tag="ACHUPT_000192"
     CDS             complement(37046..37828)
                     /gene="hisJ"
                     /locus_tag="ACHUPT_000192"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0817943.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38049..38831)
                     /gene="argT"
                     /locus_tag="ACHUPT_000193"
     CDS             complement(38049..38831)
                     /gene="argT"
                     /locus_tag="ACHUPT_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0817944.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39097..39666)
                     /gene="ubiX"
                     /locus_tag="ACHUPT_000194"
     CDS             complement(39097..39666)
                     /gene="ubiX"
                     /locus_tag="ACHUPT_000194"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0817945.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39761..41278)
                     /gene="purF"
                     /locus_tag="ACHUPT_000195"
     CDS             complement(39761..41278)
                     /gene="purF"
                     /locus_tag="ACHUPT_000195"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0817946.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41315..41803)
                     /gene="cvpA"
                     /locus_tag="ACHUPT_000196"
     CDS             complement(41315..41803)
                     /gene="cvpA"
                     /locus_tag="ACHUPT_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0817947.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42062..42724)
                     /gene="dedD"
                     /locus_tag="ACHUPT_000197"
     CDS             complement(42062..42724)
                     /gene="dedD"
                     /locus_tag="ACHUPT_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0817948.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42714..43982)
                     /gene="folC"
                     /locus_tag="ACHUPT_000198"
     CDS             complement(42714..43982)
                     /gene="folC"
                     /locus_tag="ACHUPT_000198"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0817949.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44052..44966)
                     /gene="accD"
                     /locus_tag="ACHUPT_000199"
     CDS             complement(44052..44966)
                     /gene="accD"
                     /locus_tag="ACHUPT_000199"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0817950.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45122..45781)
                     /locus_tag="ACHUPT_000200"
     CDS             complement(45122..45781)
                     /locus_tag="ACHUPT_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0817951.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45864..46676)
                     /gene="truA"
                     /locus_tag="ACHUPT_000201"
     CDS             complement(45864..46676)
                     /gene="truA"
                     /locus_tag="ACHUPT_000201"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0817952.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46676..47689)
                     /locus_tag="ACHUPT_000202"
     CDS             complement(46676..47689)
                     /locus_tag="ACHUPT_000202"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0817953.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47755..48891)
                     /gene="pdxB"
                     /locus_tag="ACHUPT_000203"
     CDS             complement(47755..48891)
                     /gene="pdxB"
                     /locus_tag="ACHUPT_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0817954.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48990..49985
                     /gene="flk"
                     /locus_tag="ACHUPT_000204"
     CDS             48990..49985
                     /gene="flk"
                     /locus_tag="ACHUPT_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0817955.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49982..51160)
                     /locus_tag="ACHUPT_000205"
     CDS             complement(49982..51160)
                     /locus_tag="ACHUPT_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0817956.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51415..52656)
                     /gene="fabB"
                     /locus_tag="ACHUPT_000206"
     CDS             complement(51415..52656)
                     /gene="fabB"
                     /locus_tag="ACHUPT_000206"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0817957.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52815..54821
                     /gene="mnmC"
                     /locus_tag="ACHUPT_000207"
     CDS             52815..54821
                     /gene="mnmC"
                     /locus_tag="ACHUPT_000207"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0817958.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54942..55220)
                     /locus_tag="ACHUPT_000208"
     CDS             complement(54942..55220)
                     /locus_tag="ACHUPT_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0817959.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55254..55802)
                     /gene="epmC"
                     /locus_tag="ACHUPT_000209"
     CDS             complement(55254..55802)
                     /gene="epmC"
                     /locus_tag="ACHUPT_000209"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0817960.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55802..56611)
                     /locus_tag="ACHUPT_000210"
     CDS             complement(55802..56611)
                     /locus_tag="ACHUPT_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR0817961.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56611..57435)
                     /gene="mepA"
                     /locus_tag="ACHUPT_000211"
     CDS             complement(56611..57435)
                     /gene="mepA"
                     /locus_tag="ACHUPT_000211"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0817962.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57439..58524)
                     /gene="aroC"
                     /locus_tag="ACHUPT_000212"
     CDS             complement(57439..58524)
                     /gene="aroC"
                     /locus_tag="ACHUPT_000212"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0817963.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58559..59491)
                     /gene="prmB"
                     /locus_tag="ACHUPT_000213"
     CDS             complement(58559..59491)
                     /gene="prmB"
                     /locus_tag="ACHUPT_000213"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0817964.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59657..60208
                     /gene="smrB"
                     /locus_tag="ACHUPT_000214"
     CDS             59657..60208
                     /gene="smrB"
                     /locus_tag="ACHUPT_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0817965.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60312..61202)
                     /locus_tag="ACHUPT_000215"
     CDS             complement(60312..61202)
                     /locus_tag="ACHUPT_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0817966.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61189..61713)
                     /locus_tag="ACHUPT_000216"
     CDS             complement(61189..61713)
                     /locus_tag="ACHUPT_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0817967.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61710..62180)
                     /locus_tag="ACHUPT_000217"
     CDS             complement(61710..62180)
                     /locus_tag="ACHUPT_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0817968.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62177..62725)
                     /locus_tag="ACHUPT_000218"
     CDS             complement(62177..62725)
                     /locus_tag="ACHUPT_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0817969.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62730..63452)
                     /locus_tag="ACHUPT_000219"
                     /pseudo
     CDS             complement(62730..63452)
                     /locus_tag="ACHUPT_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63472..66112)
                     /gene="yfcU"
                     /locus_tag="ACHUPT_000220"
                     /pseudo
     CDS             complement(63472..66112)
                     /gene="yfcU"
                     /locus_tag="ACHUPT_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66194..66757)
                     /locus_tag="ACHUPT_000221"
     CDS             complement(66194..66757)
                     /locus_tag="ACHUPT_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0817970.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16698 a        18152 c        16685 g        15470 t
ORIGIN      
        1 actccctctg attattttta ccaaccctgg gtcactgcat tatcgccaac gccccgacac
       61 gtaagtcctg agcaattaga aaaaacagtt cgttatctta cacaaactgt gcatccacgt
      121 aatgccggca atattgataa tctgaatagg tccgcagagt atataaaaga gttctttgtc
      181 agtagcggtg ccagagttac cgcgcagggc gtccccgatt ggctattacg actctgcgcc
      241 tggaagccag aattaccctt acccggcaat gtcctggctt tatcccgatc ggggagactt
      301 tattgctgtg gtcggcagaa tgcaggatat caacgccgtt cgtcaggtaa aagcggcatt
      361 attgtcatcc cgggatttat ctgtttattc tatgaatgcc ccagggttta ttcccggtat
      421 tgatttttct gatcacctca attattggca acacgatatt cccgccgtaa tgattactga
      481 caccgctttt tatcgtaata aacaatacca cttgcccggt gataccgcag acagattgaa
      541 ttatcagaaa atggctcagg tggtagatgg tgttataacc ttgttataca acagtaaata
      601 ataaaaattt ctcgaggcgg gtccagaaac agcgacatgt acattttatt ttccctcgtc
      661 tgttttaaga tgcgaaacat aaaattcaat ggaggaaaaa taaatgggaa tgattggcta
      721 ttttgcagaa attgactcag aaaagattaa ccaactgctt gagtctactg aaaaaccttt
      781 gatggataat atacatgaca cgctttcagg attacgccga ctggaatcac tggatcgaag
      841 tgagctgcgt aaacagtttt ccattaagcg actcaatgaa atggagattt atccgggagt
      901 gaccttttca gaagagttag aggggcaact ctttgcctcg atcatgcttg atatggaaaa
      961 gctgattagc gcgtatcgac gtatgctgcg acaagggaat catgcgctga cggttattgt
     1021 tgggtgatgg tgtcagagcg ccaaaaaaaa ccgccgaaca tgtcggcggc tttctgactt
     1081 acaaactaac agtttacatc agcggcattg ctaaccgcac aatactaatc agcggttgtg
     1141 gccatacacc cagcaccagt accaacagtg cagaaatcag caccacgata ccgcccgcgc
     1201 tgtactgcca gtttgacggt gcatcgcgac ccggttgttc cggtgcgtgc aggtacaggc
     1261 tcaccgccac gcgcaggtag tagtagaggc cgattgccga accgacaacc acggcaccca
     1321 ccagccacca caagtgtgcc tggacaccga ccgccagcac gtagaactta ccgataaagc
     1381 ccagcgtcat cgggataccg gccagagaca gcatcatcac cgtcatcact gccgcgagga
     1441 tcggacgatg ccagaacaga ccgcggtaag agaacaggga atcagcatcc gggccacgat
     1501 acgggctgga catcaggctg accacgccga acgcgccgag gctgctgaac agataaccgg
     1561 ccaggtaaac ccctaccgct tccatcgaca tctcgccggt ttgcagcgca atcagcgcca
     1621 ccagcagata gccgaggtga gagatagatg agtaaccgag cagacgtttg atgttggtct
     1681 ggctcaacgc catcaggtta ccgaagatga tggaggcaaa ggcgataatc gccagtacta
     1741 cgcgaatcgc ttcgctgtca cccaccggtg cgtagaggaa cagacgcatc accacaccga
     1801 agatagcgat tttgctcgcc gtcgccagga aagtggaaac cggcgcaggc gcgccctggt
     1861 atacgtctgg cgtccacagg tggaacggca ccagagagag tttgaagccg aggccaacaa
     1921 tcatcatgcc gaaacctgcc agcaacagcg gctcgttgag cataccgtcg ccgaggtttt
     1981 tacccagcgc gacaaacgac aggtcgccag actgcgcata caccagcgcc ataccgaaca
     2041 gcaggaaaga agacgctgcg gcagaaagga tggtgtattt gatactggct tccagcgaac
     2101 gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg caaagagatc agttcaatac
     2161 cgaggaacag agacgccaga tggtttgcat tcgccagcag gatcccgccc agcgcggcaa
     2221 ttaacaccaa caggtagaac tcatccttgt tgtcgttata gccttcaagc cacgggtagg
     2281 cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc ggtgtaaagc atggcgaaac
     2341 catcaacgcg catcagcggc gtaacgtcca tagcgcccgc ctggccaaca aaccagagcg
     2401 aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc gttgaggaaa tgattgcgtc
     2461 gccacgcaat ggagagcatc acaaccacca ccgtcaagcc gacgatcagc aacggtagca
     2521 gtgcgatcag gttttgtgga gttattgtca tggcgattta cggccttgta gtagtaacgg
     2581 aattaacaaa ccactgctgg atattgccaa tcgcggagtg cgaggtatcc agaatcggct
     2641 gcggatagaa gcccagcagt accagcagca ccaccagcaa caggatcata aacagctcac
     2701 gcagcgacat ccctggcagt tcctggctgg caatctggct tttcgcttta ccgaagtaag
     2761 cgcgatgcaa catcgccagc gaataaacag atgcaaagac cagcccaaag gtagagataa
     2821 cggtaatcac cgggacaacc tggaagctgc cgaacagaat cataaattcg ccgacgaagt
     2881 taccggtgcc aggcatccca agcgttgcca ccgcaaagaa cagcgacagt gctggcagcc
     2941 atttcatctt gctccacaga ccgcccatca tgcgcatgtc gcgggtatgg atacgttcat
     3001 aaagctgacc gcagagaata aacagacccg ccgccgacaa gccgtgcgca atcatctgga
     3061 ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat tgcaatcagc acgaagccca
     3121 tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc ggtctgagcg aaggtcatcc
     3181 acgcaccgta gaagatgccg ataacaccca gccacatagc gattggcgcg aactctgccg
     3241 acgcgttcgg gaacagcggc agggagaaac gcaacaaacc gtaagcggcg gttttcagca
     3301 agatccccgc gaggtccacg gaaccggcgg tcggagcctg ggagtgcgca tccggcagcc
     3361 agccatgcag cggaaccacc ggcattttga ctgcgaaggc gatgaagaag cccagcatca
     3421 acagatattc cacaccactg gacattggcg tattcagcag ctcttcatag ttgaaggtcc
     3481 agacgccggt cgcattgtag tgaacaaaaa ccagcgccag gatggcgatc aacatcacca
     3541 gaccactcgc ctgggtgtaa atgaagaact tggttgccgc cgtgatacgc gttttaccgt
     3601 cagaggcttt atgcccccac agtgcgatca ggaagtacat cggcaccagc atcatttccc
     3661 agaagaagaa gaacaggaac atgtcgatgg caaggaacac gccgataacg ccgcccagga
     3721 tccacatcag gttgaggtgg aagaagccct gatatttttc gatctctttc cacgaacata
     3781 gtaccgccag cacaccgagc agaccggtca gcacgaccat cagcagcgac agaccgtcaa
     3841 tggcgagatg aatagagata ccaaaacgcg ggatccacgg catgtcgaat tcagactgcc
     3901 actgcggaat tccggcggat tgcgtcagtg aataaccgcc ctgcaaccac agttgcagcg
     3961 acagcgccag cgtcaatccc atggtgatca gcgcgatcca gcgtggcacc ttgacgccaa
     4021 agcgttcggt ctgccagcac aggaagccgc caataaaggg aattaatatt agccagggta
     4081 gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag gaccggattt tcaacgaatt
     4141 ctcacgacag aaaattctct tcggactggg ggtagtgtcg gatgcgccgc gaaggcagct
     4201 tatccgacct acgttcttat atgcccaggg gcaatcccac aatccttaac tcaacgcagt
     4261 accatcaaca gtgccagcac cacgaccgca ccgatgctca tggatgccac ataccagcgc
     4321 agataaccgt tctcgcttaa cagcagacct ttacctgcaa agcgggaaag aaccgccggg
     4381 atgttcatca ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc caggaacggc
     4441 ttgacgaaca ctttgtcgta cagccagtca aagccccaag cgttgtacca ccaggtactc
     4501 agcaggcggc ccggcgcact gttggcgatg gaggtcacca gagaacgttt acccagccac
     4561 agccaggctg ccagcagaat gccgaccacc gcgaccacgc cagaggtaat ttccagggtc
     4621 aacatgctgc cgtgcgccag ttccgtcgtt tgcggaagca cgccctgcag cggcggtaca
     4681 atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa tcagcggcag gctgtgagtt
     4741 acccctttca cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat gaaaatcata
     4801 cggaaggtgt agagcgaggt cataaacgca ccgaccagac ctgccaccat cagattgata
     4861 tgaccattcg ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc cgcagtgacc
     4921 agcggcagtg ccgacagtgc tgcgccgccc accaggaagc agagataaac cagcggaata
     4981 gatttacgca gaccgcccat cttgaagatg ttctgttcgt gatggcaggc cagaatgacg
     5041 gaaccggatg ccaggaacag cagcgcttta aagaacgcgt gagtcatcaa gtggaaaatc
     5101 gccgcatccc atgcctgcac gccaagcgcg aggaacatgt agccaatctg gctcatggta
     5161 gagtaagcga gaacacgttt gatgtcggtc tgtaccagcg cggcaaaacc ggccagcagc
     5221 agcgtaaccg ccccgacaat acccaccaga tgcagaactt ccggcgtcat caggaacagg
     5281 ccgtgggtac gggcgatcag gtagacaccc gcggttacca tcgttgcggc gtggatcagc
     5341 gcggagacag gcgtcgggcc cgccatcgcg tcagcaagcc atgtctgcaa cggcaactgc
     5401 gcagatttac cgaccgcacc gcccagcagc atcagcgtcg cccacatcag catgttattg
     5461 ccgtcagcaa agtgcgctgg tgccagttcc accatatcgc ggaagttcag ggtgcccagt
     5521 tcgttgtaaa gaatgaacag tgcgaaagcg aggaaaacgt cacccacacg ggtcacgacg
     5581 aacgctttca ttgctgctgc gccattcttc ggatcggtgt aatagaaacc gatcagcaga
     5641 taggagcaca ggcccacgcc ttcccagccg aggtacatca gcagcaggtt atcggcgaga
     5701 accagaacta ccatgctggc gatgaacagg ttggtgtagg cgaagaagcg agagtagccc
     5761 tcttcaccgc gcatgtacca ggaggcgtac atgtgaataa ggaaacccac accagtgacc
     5821 accgagagca tggtcagcga caggccgtcc agcaccaggt taaaaccgat gttaaagtcg
     5881 cctaccgaca tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt agcgaagaaa
     5941 tcaacgccga taaaggcggt taccagcgcc gccaggccca cagagccaac gccgacgatc
     6001 gccgaaacgt tttcagacca gcgcccacgg gagaacgcca gcaggacgaa gccaatcaat
     6061 ggcaaaataa tggttaaggc aagcatgttc atccgcgcat ctcacttact gaatcgatgt
     6121 tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc aaggccgata ctcgcttctg
     6181 ccgccgcgag gctgatggcg agaatgtaca tcacctgacc gtcggtctga ccccagtagc
     6241 ttccggcgac cacaaaggcc agcgcggagg cgttaatcat gatttccaga ccaatcagca
     6301 taaacagcag attgcgacgg ataaccagac cggttaagcc aagaacgaat aagattgccg
     6361 cgaggatcag tccatgttgt aaggggatca tgcgtgctcc tccgtttttc ttttcgcgct
     6421 gtcgtcttta cgattgctca gcacttcacc cgcacgctct tcacgaccga cgtggaaggc
     6481 cacaaccaga cccgcgagca gcagcataga agccagttcc accgccagta cgtaaggccc
     6541 gaacagcgta atacccactg ctttagcact gattggcgta ccgtcgatac cctgatcgtt
     6601 aacaccgagg atggcgtaaa caatcaccac cagcatgatg gccgacaaaa ttgccgggcc
     6661 aatccacacc tgcggtttca gccactggcg ttcctgttcg atttctgaac cgcccaggtt
     6721 gagcatcatc accacgaaca cgaacagcac cataatggca cccgcgtaga cgataatttc
     6781 cagcgcaccg gcgaagtaag cgcctagtga gaagaacacc ccggaaatcg ccagcagcga
     6841 aataatcagg tacagcagtg cgtgtaccgg attggtatgg gtgatcactc gcaaggtcgc
     6901 aagtatggct atcaggccac agatataaaa agcgaactcc attgcccctc tccttacggt
     6961 aacaggctct tgacgtcgat aggcttggct tcgttctctg cttcgccctt atctttgccg
     7021 tcgattgcca tacctgccat ccggtagaag ttatattccg ggtatttgcc cggaccggag
     7081 atcagcagat cctctttctc gtaaaccaga tcctggcgct tgtattcccc catttcgaaa
     7141 tccggggtta actgaatcgc cgtggtcgga caggcttctt cgcacagacc acagaaaatg
     7201 cagcgtgaga agttaatgcg gaaaaattcc ggataccagc gaccgtcttt ggtttctgct
     7261 ttttgcagcg agatacagcc gaccgggcag gctaccgcac agaggttaca ggctacgcaa
     7321 cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac cacgataacg gggcggcaga
     7381 tagaccggct cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg caggccgatc
     7441 atccagatac tacgaacctg ggtgccgaaa cctactaaca attcttttaa ggtcatggtc
     7501 ttattgcccc ttattgcgcc tgccagagaa tgacagccgc cgttaccagc aagttgatca
     7561 gcgtcagcgg caggcagatt ttccagccga aggacattac ccggtcataa cgcggacgcg
     7621 gtaacgacgc acgaatcaaa atgaacatca tcataaagaa cgcggttttc agcgcgaacc
     7681 agatgaatgg cggtaacaac gggccttgcc agccaccgaa gaacagcgtc accatcaatg
     7741 cagagatggt cacaatcccg atgtactcac ccacgaagaa cagaccgaac ttcataccgg
     7801 aatattcaat gtggtaacca tccgccagtt cctgctcggc ttccggctgg tcaaacgggt
     7861 gacggtgaca caccgccacg cccgcgatgg caaaggtaat aaaaccaaag aattgcggga
     7921 tgacgttcca cacatgcgcc tggctgttga cgatgtcggt catgttgaat gaaccggcct
     7981 gcgccaccac gcccatcagg gaaagcccga ggaacacttc gtagctcagg gtctgcgcag
     8041 aagcacgcat cgcacccagc agcgagtatt tgttgttact tgaccagccc gcaaacagca
     8101 ccgcgtaaac cgccagacct gccatcatca ggaagaacaa aatcccgatg ttcaggtcgg
     8161 caaccaccca acccggactg actggcacaa ttgcaaaggc cagcagcagc gaggtaaagg
     8221 caatcatcgg tgccagggta aagatgacgc gatccgagaa tttcgggatc cagtcttctt
     8281 taaagaacat tttgatcatg tccgcaacca gctggagcga accgccccag ccaacacggt
     8341 taggtccata acggttctgg aacagaccca gcaggcgacg ttcgccaaag ctcatgaatg
     8401 ccccacaggt gacaaccacc agcaggatca ccaccgcttt gaggatggtc agcaggatct
     8461 caatcagttc cggtgatatc caactcattg ttgtgcctcc ttgagatcct caagatgcgc
     8521 gccagccagc accggagcaa tgccggacat acccatcggc aagcccacct gccctgccgt
     8581 cagtccttcg gcgatttcaa ccggcagcgt gaccgtgttg ccatcgtaac taaaggagac
     8641 gcgtgtacct gcgttcacac ccaacttcgc ggcatccgct gggttgagtt tgatgtacgg
     8701 ctgcggcata cggctctgga agaccggagc acgctgtgac aattcatcgc tgccaaacag
     8761 gtggtagtac ggcgcgatac gccatttccc gtcctgcggc tggaagcgtg ccggtacgct
     8821 ggtgaagtaa tccagaccgt tttcgctggt ttcaaacaga cgcacgcccg gatcgccaaa
     8881 gcgcagtttg ccgcccactt cgtcctggaa tttgttccac gcctgcgggg agttccagcc
     8941 cggcgcccag gcaaacggca cttgcgaacg gtgcgcggtc ggctggttgt taccttccat
     9001 cgagaaggtg aacatggtgt caatatcctg cggctgacgc ggctcatgaa cgctgatatt
     9061 ggcgcgcatg gcggtacgac cgctgtaacg gtgcggttca cgggccagtt tctgcccacg
     9121 aatacggaat gtcgcatccg gcgcagcatc tttgataccc gccagttctg ggattttcgc
     9181 cacaacagcg tcaattacat ggtcgagctg cgtccagtcc acttcacggc tcagcaaggt
     9241 gctgtgcagc gagtgcagcc agcgccagct ttccagcatg acagttttgc tgtcgtaata
     9301 agccggatcg taaacctgga agaaacgttg ggcgcgacct tcattattaa ttaccgtacc
     9361 gtcgctttca gcaaagctgg cagaggaaag caccagatgg gcgttttcca tgatcgctgt
     9421 gcgttgatga tcaaccacca tcaccagcgg cgctttagcc agcgcagcat tcacgcgggt
     9481 agcagaagcg taacgatgca gatcgttttc caacaccacc accgcgtcgg cgcgtccggt
     9541 ttccagttcg gttaacgctt cttcaagcga accgccaccc ataatgccca gccccatgct
     9601 gttgacggaa cgggcaatca tggtgatacc aacgtcagcg ccgcgacctt tcagggcttt
     9661 cgcgacgtta gccgcagcct gaatcacctc tatgctaccg gcgttcgtcc cggagataat
     9721 caacggtttc ttcgcacctg ccagtgcctg cacgatgacg tcgattttgt tttgcagctc
     9781 aggttcgata ccgtcaaccg ctggtgcgga gttatccagc gcatgggcaa tggcaaaacc
     9841 taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat catccagacg
     9901 agtgtcatca acgttggtaa caaacagcgg atgcttcgca cgttgaccga tgttgaggat
     9961 tgccgcaatc tgccagtcag ccactttctg tgctgcagcc atttcgcgcg ctttaccttt
    10021 caccgcctga cgcactgcca gtgcgacgcg cgcgccggtc tgggtaacgt cttcgcccag
    10081 caccagtacc gcatcgtaag attcgatttc gcgcagagcc ggagtataaa tgccgccttc
    10141 acgcagcact ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga taccggtgta
    10201 gaagttttct tcgcccacca gttcacgcag cgcaaagttg ctttccacgc tggcacgcgg
    10261 agaaccaata ccgatcactt tcttcgactg acgcagaata tctgccgcgc cctgcattgc
    10321 ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc tgtactggct gacgcggacg
    10381 atccttcagg ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga agtagtggtt
    10441 taccgtaccg ttgtaacggt tttcgatacg acgcagttcg ccgtaacgtt caccggggct
    10501 gatgttacag ccgatggaac attgctggca gatgctcggc gcaaactgca tatcccattt
    10561 acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc aaatttcgac
    10621 caggttaccg gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga agtagacgtt
    10681 gtcgtgcgcg ccataaacgc ccagatctgt accgtcagcg taatctttgt agtaacgcac
    10741 acagcggtag caggcgatgc agcggttcat ttcgtgagag atgaatggcc ccaaatcctg
    10801 attacggtgg gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg tcatcacggt
    10861 catatcctga agatggcagt taccgccttc ttcacatacc ggacagtcgt gcgggtggtt
    10921 ggtcattaac cactcgacca cactttcacg gaactgtttc gcttcttcgt cgtcaatgga
    10981 aataaaggtg ccatcggaag ccggtgtcat acaggacatc accaggcgac cacgcgtgtc
    11041 ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc ttcccagcgc
    11101 cggatgccag caaaagtaag gaatatcaag gcccagagac agacaagctt ccagcaggtt
    11161 gtccgctccg ttgacctcgt attctttgcc gtctacatga attgtagcca ttagcatgct
    11221 tccagttttc tcggtcaggg accgagcgtt aatcgaaatt cggttaccag cgctctttca
    11281 gcaggttcgg ctgaatccca ttaatcaaat gggtattgct gaacggctgt ttgattcccg
    11341 cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa cggctccact gcaccaggtg
    11401 cgtgggcaca gaaagtttta cccgggccta agaatcgaca cagttgctca agtgtttcga
    11461 tatcgcccgg ctggccttcg ccacgctcca gcgcacgcag aattttcacg ctccacggca
    11521 gaccgtcgcg gcacggcgta caccagccgc aggactcacg ggcgaaaaac tcttccaggt
    11581 tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc catcgccagc gccgtaccca
    11641 gacggctgcc cgctttaccg atactttcga attccatcgg cagatcaagg tgcgcttcgg
    11701 tcaggaagtc agtccccgcg ccgcctggct gccaggcttt aaatttcaga ccatcacgca
    11761 taccaccggc gtaatcttcg aggatctcgc gtgcggtggt gccgaacggc agttcccaca
    11821 gtcccggatt tttcacccga ccggagaagc ccatcagctt ggtgccagca tctttacttt
    11881 tcgagatgtt ctgataccac tccacgccgt tagcgaggat cgccggaacg ttacacaggg
    11941 tttcgacgtt gttgacacag gtcggtttgc cccatacgcc ggaggttgcc gggaaggggg
    12001 gtttcgagcg tgggttagca cgacgccctt ccagggagtt gattaacgct gtttcttccc
    12061 cgcagatgta gcgccctgcc ccggtatgga cgaacagttc gaagtcgaaa cctgttccca
    12121 taatgttttt gccaagcaag cccgcttcag tggcttcggc aatggcgcgg cgcagattta
    12181 ctgccgcttc gatatattcg ccacgcagga agatgtagcc acggtaagct ttcagcgcaa
    12241 acgcggagat gagcatacct tccaccagca ggtgcggcag ttgctccatc aacaggcggt
    12301 ctttataggt gcccggctcc atttcatcgg cgttacacag caggtaacgg atgttcatgg
    12361 attcgtcttt cggcatcagg ctccacttca ggccagtcga aaagcccgcg ccgccgcgcc
    12421 ctttcagacc agcgtctttt acctgattaa cgatttcgtc cggagacagc ccggtcagcg
    12481 ccttacgcgc cccttcgtaa ccgtttttgc tgcggtattc gtccagccac actggctgtt
    12541 tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg agtacggata atgtttttca
    12601 tttataccgc tccagcagtt cagggatcgc ttccggggtc agatgcgcgt gagtgtcctc
    12661 atcgatcatc atgtttggcc ctttatcaca gttccccagg cagcaagttg gcagcagcgt
    12721 aaagcggcca tcaaatgtcg tttgccctgg tttgatgttc agctttttct cgagcgccgc
    12781 ctgaataccc tgatacccgt tgatatgaca gaccacgctg tcgcaataac ggatcacatg
    12841 gcgaccaacc ggctggcgga agatctgact gtagaacgtt gccacacctt cgacgtcgct
    12901 tgccggaata cccagcacat cggcgatcgc gtggatcgca ccatccggca cccagccacg
    12961 ctgcttctga acgattttca gcgcttcaat ggacgccgca cgcgggtctt cgtagtggtg
    13021 catctcgtgc tcaatcgctt cacgctctgc cgcactcagc tcaaaagcct cggtttgtgg
    13081 ttgttgattc tcgtgcataa ttagcggtcc acatctgaca taacaaaatc gatactgccc
    13141 agataaacaa tcaggtcaga caccaggctg ccgcggatcg ccgccggaat ttgctgcaaa
    13201 tgcgcataac tcggcgtgcg gatacgagtg cgataactca tggtgctacc gtcgctggtc
    13261 aggtagtaac tgttgatccc cttggtcgcc tcaatcatct ggaaagattc attggcaggc
    13321 atcaccggcc cccacgacac ttgcaggaag tgggtgatca gggtttcgat atgttgcagc
    13381 gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg ctttgaacgg gccttccggc
    13441 atgttgttga ggcactgctc aagaatgcgc agactctggc gcagctcttc cactttcagc
    13501 attacgcggg tgtagcagtc agaaacacca ccaccaaccg ggatttcaaa gtcgaagttt
    13561 tcatagccag aataaggacg cgccttacgc acgtcgaagt cgatcccggt agcacgcagg
    13621 cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc cataggcggc aacgccctgg
    13681 gaacgacctt tcagaatggt attttgcagc gccgctttct cgtaagacgc cagacgtttc
    13741 ggcatccagt cgaggaactc acgcagcaga cgatcccagc cgcgcggcag gtcgtgcgct
    13801 acgccgccaa tacggaacca cgccgggtgc atacggaaac ccgtgatcgc ttccaccaga
    13861 tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc gccgacgtcc
    13921 tgaataaagg tcgagatgta cagcaggtgg ctgttgatac ggaacagttc ggagagcata
    13981 acgcgaatga cgttaacgcg atccggcacg gtgatcccgg ccagtttctc taccgccagc
    14041 acgtaaggca tttcgttaac gcagccgccg aggtattcaa tacggtcggt atacggaatg
    14101 tagctgtgcc aggactggcg ctcgcccatt ttctccgcac cacggtggtg gtagccgatg
    14161 tctggtacgc agtcgacaat ctcttcgccg tcgagttgta aaacgatacg gaaagccccg
    14221 tgcgccgacg ggtggttcgg accgaggttg aggaacatga agtcctcatt ttcggtgccg
    14281 cgtttcatcc cccactcttc cggtttgaag gtcagggctt ccatctccag atcctgtttg
    14341 gctttggtca gctcaaacgg cgagaattcg gtagcgcgtg ccgggtaatc tttacgcagc
    14401 gggtgacctt tccaggtttg cggcatcatg atgcggcgca ggttcgggtg accgtcgaaa
    14461 gtaatgccaa acagatccca ggtttcacgc tcataccagt tagcgttcgg gaacagtttg
    14521 gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca ccttcagcat gatgtcgcgg
    14581 ttacgatcga tagaaatcag atggtagaaa acggaaaaat ccgcggcagg taacccttcg
    14641 cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa acagcatgac gtaaggtttc
    14701 ggcagtttct ttaagaaatc gccaacttcc agtaattgtt cacgcttgat ccacacaacg
    14761 ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg gttgcgcagt
    14821 tcgccaatta ccggatcatc aagatgatcg cgggtctgcc aggcgggttc ttgcgcggtt
    14881 aagtcagtca tattgttcac cattgcaaat ggtccgtggt gactgtcggc gcagtaagct
    14941 tcgcgaaata gatttaggaa taagcgaaga aaaatccctt tgccgacagg cgcaaattaa
    15001 atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt gcgttcgcgc
    15061 tctgattgca tattggcgcg ataaacgccc tgatcgccaa ccacccagga gagcggacga
    15121 cgttctttgc cgatagattc ctgcaacagc atcagtgcct gcatgtacgc ttcaggacgc
    15181 ggcgggcagc ccgggatata cacatcaacc gggatgaatt tatcgacgcc ctgcacaacg
    15241 gaataaatat cgtacatacc accagagttg gcacaggcac ccattgagat aacccatttt
    15301 ggttccagca tctggtcata cagacgctga ataaccggtg ccattttggt aaagcaggtt
    15361 cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc tgcgccaaaa
    15421 cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca gcaggaaaga
    15481 ccgaagttat acggccaaat tgagttttta cgaccccagt taaccatgtc attgagcttg
    15541 cccataaaca cgtttttgtt aacttcttgc tccagagggt cggttacgat ctcctgcttt
    15601 tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt ataatccatc
    15661 ttaatgcctc gcggttagcg ttgacgatta gcgatactgt tcgtttccgg gttcatacgc
    15721 tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag ataaaccaga
    15781 cctgccagta acacaaaaat aaaaattgca gcttccacaa agcctaccca gccgctttcg
    15841 cggatagagg ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat aacgaagaac
    15901 atggccacca gataaaactt ggcggacagg cgtaagcggg cggagccgac cgagtcgata
    15961 ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa aaaccaaccg
    16021 cctaccagca tcaggcaaca caggccaatg gcaacgataa gaaagatagc gaatgcccag
    16081 tgatgagcga tgacttcagt ggatgttgac atactcattg cttactcatc aaaagtagcg
    16141 ccagattcac tgctcttcac ggcagatgga cgccacatcg attcatgggg aggaataaaa
    16201 aaaaccttac aataactgta gaaattcttt tatacagcta attgatgtgg tcttttactc
    16261 ctttctataa ccttttgtca actttaacaa aagcttcttc acattagttt acataatatc
    16321 aacaccatta gcatttaatg ccctttcacc ccagatcctt gacgactcca ggataattag
    16381 atgttgttga atcgtgtccg ttgtgaagca atggaaaaaa tacgggtcta ttttgacagg
    16441 aatttgtgtc gattcctccc cccaaaagag agtattttct tgatctgtga cacgcttttg
    16501 ttattccata acaaaaacgc agcaacaaat ttacgtattt tttaacatca ttgtagcagg
    16561 tgattttttt caggcgatta tttgtgcatt cgggacgtga atctctggtg attgaaaaat
    16621 aaacagtttt gtacgttctg cactatgcga ggaaggattt ttactaaaaa aaagccgctg
    16681 gggtttaaaa cacccccagc ggctcgtttt ttacactatt gtctcaggaa ttatctatcg
    16741 tccgtcgatt actcgatatc cctttcaatc aacaatgaat catccccttc cggagcagac
    16801 attgggctgt attgccacgg attatggtag ctctccatcg cctgatagat cacctgggcc
    16861 agctcattat tactggacgg gtcatagcac agcaaatatt cggtatcagg tagcggcggt
    16921 aagccatcta ctccactcag cacacgcagg tccgggctca tcatctcaac cggccttgcc
    16981 gtcacgccaa gaccggcttt cactgccgca cgaacggccg gaagcgtcga ggcgacataa
    17041 gccagtcgcc atggaatatc tactttatta agcgtcgcca gcaccatatc gcgaaacgga
    17101 cttggatcat ccagcaatac aagagggatc ggctcgcctt tttgcaatac gtattccgct
    17161 gcgcagtacc agtgtgttgg cgaggtacgc aggttcaatg ccttaaacgc tgaaggacga
    17221 tgggtggtta ccatcaaatc cacttcctgg gattccagca tttctgccat ataggcatta
    17281 cgtttaacgc ggacatccag cgctaatttc ggataaaccg aactcacgcg atttaacagg
    17341 aaaggtaaga tcgtatcggc agattcatct gaagcaccga tagttaacac gccctgaaga
    17401 ttactgaaca ttaatgatga gcaggcctca tcattaaaac gcaggatttt cctggcgtaa
    17461 ccaagaagtt gaatgccatg ttcagttaac agtttgttgc gaccgtgacg agcgaacagt
    17521 tctttcccaa cgagttgttc cagacgctgc atttgctgac ttacggcgga ctgagtacga
    17581 cacacagcgg cagctgcggc agcaaaagtg ttcagatcgg caacagcaac aaatgttctc
    17641 agcagatcga ggtcgaggtt aattatcgga cgatttgcac ttatcatata ttatcactta
    17701 ctggcggctc atactgagct ggttaatgct gtgcacacac aaacaagcaa ttccatttgt
    17761 aatgtgcctc cctggcagtt tcatccggaa cccggacgaa agtaaaaatg catatgagtt
    17821 gcactaaaaa agcgactcac attgttccgt tataatgcct gaagtagatc acagaatata
    17881 tcttcaggga tcgcatatct attaagttac tcactctttt ctatttatga catgcgcgtg
    17941 tttgtataaa tgtaaatgtg agtccttgtt ccactctcgt gcagcatcgc tggtcatacg
    18001 cgaacacgta caaacagcaa tggtgaagcg gcatcaagag cagggatccg tcatttatgc
    18061 gagcatttta ccccaaaaac cttttattta taagggtcat tgcgaattat ctgatgcaaa
    18121 gttatgttag gcaaagtaat cttctattat taataagcac atcaaaacct ttttgaatat
    18181 taaataataa ttaattagca tcatcctcat tcattaattc cgcttaacaa tagtttcaca
    18241 ccttaccccc attgcgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga
    18301 aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct
    18361 tacagataaa aaaaccagac ttgcaattaa gaattagaac aattcactat ataacattgc
    18421 atgtaaagca tataagccta attatttgtc attattaagt tattaacagc acaatcgagc
    18481 cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca
    18541 ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc
    18601 atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg
    18661 gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc
    18721 ggcaacccag ccccattcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc
    18781 aacctgccta ccgctcaagg gtattgcgat tccaaagggc tttactccgc gcgtaaagcc
    18841 atcatgcagc actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc
    18901 ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac
    18961 gaaatgttgg ttcctgcacc agattaccca ctctggaccg cggcggtttc gctttccagc
    19021 ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat
    19081 attcgcgcta aaattacgcc tcgtacgcgt gggattgtta ttatcaaccc aaataaccca
    19141 accggcgcgg tatattccaa agagctttta atggagattg tggagattgc acgtcagcat
    19201 aatctcatta tcttcgccga tgaaatttat gacaaaattc tctacgacga tgctgagcat
    19261 cactcaattg cgccgctggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa
    19321 acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac
    19381 gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt
    19441 cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt
    19501 acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt
    19561 ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc
    19621 aaacgcttta acattcatga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa
    19681 gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc
    19741 acgctaccgc gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct
    19801 ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc agtgttatcc
    19861 cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt
    19921 ttgcccatct ctcccgcctg aaactcatta accgctggcc gctaatgcgc aacgtgcgga
    19981 cggaaaatgt gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta
    20041 tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt
    20101 accacgatgc ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatg
    20161 cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcagaaa ctggtcgata
    20221 tggttccgga agaactgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg
    20281 atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg
    20341 aagaactcgc ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc
    20401 ttgaagcgcg tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc
    20461 atctttcgct cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg
    20521 catcagtcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg
    20581 gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa
    20641 gtcactgaag ctgtaatttc ccggacctaa aaccagggtg ttaacaggtg aagacaccgg
    20701 tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcaaatggat aaggcgacac
    20761 tcccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt
    20821 agagataaac agcccaataa ccgccgacaa gacaaacaaa caccccagca tcatatgtgg
    20881 cccataaccg ccgccaatat ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt
    20941 ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat
    21001 ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat
    21061 gatagcggca acaggattag gaatttcatt tgtcagcatt aacgccacca ttagcaccag
    21121 acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg
    21181 taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt
    21241 acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag
    21301 cgccacgcca ttgcgcttga gccccaccac attcagtccg tagcgggtac gaaaaccaat
    21361 ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc
    21421 cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg
    21481 ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc
    21541 acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca
    21601 gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg
    21661 ttgacctatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg
    21721 atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg
    21781 ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac
    21841 gaccaggcca ataggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa
    21901 ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag
    21961 cccggcaaaa cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac
    22021 gcttaacacc acgggaataa agatagcgac aacgctggtt gaactcataa acgcgccaag
    22081 ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgccacttt
    22141 gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat
    22201 aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac
    22261 cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg
    22321 cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa
    22381 ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa
    22441 agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca
    22501 tttggctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc
    22561 aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgccaggcca
    22621 gaaagcacgc cagcgggagc atcttccacc accacacact cctgcggcgc aagccccagc
    22681 agctgcgcac ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg
    22741 gttacaaaca cctctggtgc gggaagcccc gctattttat ggcgcgctcg cgctaccggc
    22801 atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg attgagtaag
    22861 gcgattgccc caggaagcgc ggtaatacct tcggtttccg tggcctcgat ttgctccaga
    22921 cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcaaagag
    22981 gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt
    23041 ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca
    23101 agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt
    23161 aatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg
    23221 ctgatatttt tcccacattg gcgtctgggc gttaaaacca tgagtcccag cgtcaaaatg
    23281 ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt
    23341 ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa
    23401 attagaccaa gaaacataca acgcatgata catatccata atgtcgatga tagtgcggca
    23461 ggtttcttct ttcagctcgc caaactcgcg atccaattca cgcatctgta atccgtaacc
    23521 acgctcaata attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt
    23581 catcatcttg tactgattag acaaaataag acgttgcgcg ttggtcattt ccattgttga
    23641 ctcctgtatc actctactac ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg
    23701 cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc
    23761 gcttaagcgt gtcagctaat gcctggtaat caggcttgcc ttcaacgggt gccaacacct
    23821 gtccagactc ctgcaattta ccgcgaactt cataaaacca gttaaggatt gcagggggta
    23881 atggcgttac agaacgcttg cccagccacc acaatccctg catgggtaaa cttaaggcga
    23941 acagtgcagt ggcaactgcc ggcccaagca gaccgcccag ggcaatctgc cagcagagag
    24001 taaatacggc gatcggcggc ataaaacgga tcgcataacg cgtcatcttg ataacgcgat
    24061 tttcgacaaa gaccggagca aggcgttttt ccagtggcca cgtctttgag taatgctgtc
    24121 cgcggcgaaa caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc
    24181 tcaacttcac atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt
    24241 tcaaaacatt ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa
    24301 gtagtacata ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac
    24361 gcaaaatggc atagactcaa gatatttctt ccatcatgca aaaaaaattt gcagtgcatg
    24421 atgttaatca taaatgtcgg tgtcatcatg cgctacgctc tatggctccc tgacgttttt
    24481 ttagccacgt atcaattata ggtacttcca tgtcgagtaa gttagtactg gttctgaact
    24541 gcggtagttc ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa gagtaccttt
    24601 ctggtttagc cgaatgtttc cacctgcccg aagcacgtat caaatggaaa atggacggca
    24661 ataaacagga agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg
    24721 ttaatactat tctggcacaa aaaccagaac tgtctgcgca gctgactgct atcggtcacc
    24781 gtatcgtaca cggcggcgaa aagtatacca gctccgtagt gatcgatgag tctgttattc
    24841 agggtatcaa agatgcagct tcttttgcac cgctgcacaa cccggctcac ctgatcggta
    24901 tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa aaacgttgct gtatttgaca
    24961 ccgcgttcca ccagactatg ccggaagagt cttacctcta cgccctgccg tacaacctgt
    25021 acaaagagca cggcatccgt cgttacggcg cgcacggcac cagccacttc tatgtaaccc
    25081 aggaagcggc aaaaatgctg aacaaaccgg tagaagaact gaacatcatc acctgccacc
    25141 tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa atgcgttgac acctctatgg
    25201 gtctgacccc gctggaaggt ctggtcatgg gtacccgttc tggtgatatc gatccggcga
    25261 tcatcttcca cctgcacgac accctgggca tgagcgttga cgcaatcaac aaactgctga
    25321 ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag cgactgccgc tatgttgaag
    25381 acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg
    25441 cgaaatacat cggtgcctac actgcgctga tggatggtcg tctggacgct gttgcattca
    25501 ctggtggtat cggtgaaaat gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg
    25561 tgctgggctt tgaagttgat catgaacgca acctggctgc acgtttcggc aaatctggtt
    25621 tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa gaactggtta
    25681 tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac cgccagctca gctggcggtg
    25741 ctgttttgta acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt cccgtattat
    25801 tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg
    25861 tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac
    25921 cggtggcgat gcgcccgatc agactacgac tatcgtgcgt gcgaactctt ccaccacgac
    25981 ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca atcagaaaga
    26041 tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt
    26101 tctggttgaa ggtctggtcc cgacacgtaa gcaccagttt gcccagtctc tgaactacga
    26161 aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg tctcagggca ctgacacccc
    26221 ggaacagctg aaagagcgta tcgaactgac ccgcaacagc ttcggtggtg ccaaaaacac
    26281 caacatcacc ggcgttatcg ttaacaaact gaacgcaccg gttgatgaac agggtcgtac
    26341 tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa acaatgttga
    26401 tccggcgaag ctgcaagaat ctagcccgct gccggttctc ggcgctgtgc cgtggagctt
    26461 tgacctgatc gcgactcgtg cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa
    26521 cgaaggcgac atcaatactc gccgcgttaa atccgtcact ttctgcgcac gcagcattcc
    26581 gcacatgctg gagcacttcc gtgccggttc tctgctggtg acttccgcag accgtcctga
    26641 cgtgctggtg gccgcttgcc tggcagccat gaacggcgta gaaatcggtg ccctgctgct
    26701 gactggcggc tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac
    26761 cggcctgccg gtatttatgg tgaacaccaa cacctggcag acctctctga gcctgcagag
    26821 cttcaacctg gaagttccgg ttgacgatca cgaacgtatc gagaaagttc aggaatacgt
    26881 tgctaactac atcaacgctg actggatcga atctctgact gccacttctg agcgcagccg
    26941 tcgtctgtct ccgccagcgt tccgttatca gctgactgaa ctggcgcgca aagcgggcaa
    27001 acgtatcgtt ctgccggaag gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc
    27061 tgaacgtggt atcgcaactt gcgtactgct gggtaatccg gcagagatca accgtgttgc
    27121 agcgtctcag ggtgtagaac taggtgcagg cattgaaatc gttgatccag aagtggttcg
    27181 cgaaagctat gttggtcgtc tggtcgagct gcgtaagaac aaaggcatga ccgaaaccgt
    27241 tgcccgcgaa cagctggaag acaacgtggt gctcggtacg ctgatgctgg aacaggatga
    27301 agttgatggt ctggtttccg gtgctgttca cactaccgca aacaccatcc gtccgccgct
    27361 gcagctgatc aaaactgcac cgggcagctc cctggtatct tccgtgttct tcatgctgct
    27421 gccggaacag gtttacgttt acggtgactg tgcgatcaac ccggatccga ccgctgaaca
    27481 gctggcagaa atcgcgattc agtccgctga ttccgctgcg gccttcggta tcgaaccgcg
    27541 cgttgctatg ctctcctact ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt
    27601 tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct
    27661 gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc aaagcgccga actctccggt
    27721 tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac accggtaaca ccacctacaa
    27781 agcggtacag cgttctgccg acctgatctc catcgggccg atgctgcagg gtatgcgcaa
    27841 accggttaac gacctgtccc gtggcgcact ggttgacgat atcgtctaca ccatcgcgct
    27901 gactgcgatt cagtctgcac agcagcagta atctcgtcat catccacagc tttgcgctgc
    27961 ggatatctga accggaaata atcactattt ccggtttttt attctcttaa tttgcattaa
    28021 tcctttctga ttatcttgct taactgcgct gcatcaatga attgcgccat ctcactttgc
    28081 atacttacca ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc
    28141 caaaccaaca agaagatggg caatgcccga tacgttggtg attatctttt ttgttgctat
    28201 tttaaccagt cttgccacct gggtagttcc ggtggggatg tttgacagtc aggaagtgca
    28261 gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta gatccacact catttcgcat
    28321 tctgactaac gaagcaggcg aacctgagta tcaccgcgta cagctgttca cgacgggcga
    28381 tgaacgcccg ggcctgatga acttcccgtt tgaagggtta acctcaggat cgaaatacgg
    28441 gacagccgtt ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat
    28501 gcgtacagga accattgata acggtatcct ggcgcttatt cgccataccc gcgggaatga
    28561 aattctcttt attcctgcgc tgtttattct gttttcactt ggcggcgcgg tatttggtat
    28621 gggagaagag gccgtcgcct ttgccattat catcgcaccg ctaatggtcc ggctgggcta
    28681 tgacagtatt accaccgtcc tggtgactta tattgccacg caaatcggtt ttgccagttc
    28741 gtggatgaac ccgttttgtg tggttgttgc tcaggggatt gccggcgttc cggtgctttc
    28801 tggctccggg ttgcgcatcg tggtgtgggt tatcgccact ctgattggcc tgatctttac
    28861 catggtgtac gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga
    28921 ccgcttcttt cgtgaaaagc aggcggatgt tgaacaacgt ccgtttacct ttggtgactg
    28981 gctggtattg attgtcctga cagccgtaat ggtctgggtg atttggggcg tgatcgttaa
    29041 tgcctggttt attccagaaa ttgccagcca gttcttcacc atgggtctgg tgattggcat
    29101 catcggcgtt gttttccgcc ttaacggcat gacggttaat accatggctt catcctttac
    29161 cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca aagggatttt
    29221 gctgctggtt ggtaatggtg aagcaggtaa tgccagcgtg ttaaatacta tcctcaacag
    29281 cattgccaat gccattagcg gtctggacaa cgcggtcgcg gcctggttta tgttgctctt
    29341 ccaggctgta tttaatttct tcgtgacgtc cggttctggt caggcggcat taactatgcc
    29401 gttactggca ccgcttggcg atctggtcgg tgttaaccgt caggttaccg tgctggcgtt
    29461 ccagtttggt gatggcttca gccacatcat ttacccaacc tcagcttcgt taatggcaac
    29521 gctcggtgtg tgccgggtgg acttccgtaa ctggctgaag gtgggcgcga ccctgcttgg
    29581 actgctgttt attatgtcca gcgtcgtagt gatcggcgct cagttgatgg gctaccacta
    29641 aaaatgttaa gagccgcaat tgcggctctt ttttcattct gccgtttcag tctctacggc
    29701 ttcatttttg gcattgcgct tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa
    29761 ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag caaactccac tgacttcatc
    29821 ttcctgtagg gcgaagggac cgtgagagac gcaactgaac aacgcgcccc agacacggca
    29881 atttttatct tcgaaataga actgcccgtg ctcggcaaag gggacaccgg caatgcccaa
    29941 ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc tcatcggcct gaactacacc
    30001 gcctgcggtc gcatctaaca tgccgggtaa aaagtctttt gtctcggtac gacgctggac
    30061 cagaattttg cccatgccat catgcacgac gatgtaagtt gcacgatgac gcagacactg
    30121 tgcccgcatt tgttcccggc tggcttgtgc aatgacttcg ttctcttcat tgacaatatc
    30181 cacccattca gtacttgcca aacgacgctg ttccattatc aggaaacctt ctttttctgg
    30241 cgctcttacg gcgcattttg agttgtgggt aaattacgga ttaatcgcga cctgcgcaat
    30301 gatactttga tcattgagtg cgattacact aagtacgtca ttatccagca tgccataacc
    30361 cgccggatta ccgcctttcg gaatactcac cgagccgggg ttgaagtgga aaatctcccc
    30421 ccgctgttct gccactggca gatgggtatg accgtacacc agcacatcgt tctggtttaa
    30481 agcaggtaga ttttccgggc caaaaagatg accatgcgtc aaaaacagac gttgtttttc
    30541 cagtaatacc tgttgccacg gcgcggttat cgggaaatgc agcatcattt gatccacttc
    30601 gctgtcgcag ttgccacgca cagcgataac cttatgtgcc acttcattaa gccgttcagc
    30661 gactttggct ggcgcgtaac cctccggtaa agcattacgc gggccgtgat tcaacacgtc
    30721 gccaaggatc accagccact gggcaccgct ttggacaaac aactccacaa cacgttccgt
    30781 cgccggtaac gacccatgaa tgtccgatgc aaacatcagt ttcatcactc actcctcgtc
    30841 gaaaaaacgt aagctatgat actggattct gtcgttgcta tcagccagat tgcttcgccg
    30901 aaagtgcaat aaaacgctgg acagacgctc gctgccactg gaatgtcgca taatccacca
    30961 ggcgttctgg cacctcatcg ccatgtagta ccaggcggtt aatcattagc gccagatcag
    31021 tatcagcaat gcaccattca ccaaataaat tcggctgacc cagtgctaac aaatgttctg
    31081 ccatcgcgaa cagtttctct gcactggctt ttccctcggc cgttagtggc gcttttttcg
    31141 cccccgcaaa gacaacatcc gtcggacgct cttcgcggat gggcatcaga tcgctgcgca
    31201 gccaggcctg aatctgtcgt gcacgcgcac gattttctaa atcaagcgga taaatacgtt
    31261 cccaggtcgg tggcgcaaat cgatcttcca gatactccgc aatggcagaa gattcactca
    31321 actcaaaatc atcgatttgt aataacggca cacggcgtgt ctgaccgtaa ccttgccacg
    31381 tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt
    31441 tttcctgcaa cgccacccag gcggataaca cataagggga gaaaaagtgg gcatctgacc
    31501 aaagcgtgat agcgggttta ctcattatgt cctcggcaat aaagggggct ggctatcaaa
    31561 ctatagagcc tttttgtcac tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct
    31621 atacttgtgg tgttcagatg attaaacaac cggagctgca atgatcgatc tctatttcgc
    31681 cccgacaccc aatggtcaca aaattacgct gtttctcgaa gaagcagagc ttgattatcg
    31741 cttgattaag gtagacttgg ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc
    31801 gcccaacaac aaaattccgg caattgttga tcattctcct gccgatggcg gcgaaccgtt
    31861 aagcctcttt gaggctgctg ccattttgtt gtatctggct gagaaaacag gactcttttt
    31921 gagtcatgaa acgcgtgagc gcgccgccac attacagtgg ttattctggc aggtaggcgg
    31981 actggggccg atgcttgggc aaaatcatca ttttaatcac gcagcccccc aaaccattcc
    32041 ttacgctatt gaacgttatc aggttgaaac tcagcgtctt taccatgtac taaacaagcg
    32101 gctggaaaac tcgccctggc tgggaggcga gaactacagc attgcggata ttgcctgctg
    32161 gccgtgggtt aatgcctgga ctcgcctgcg aattgacctc gcaatgtatc cggcagtcaa
    32221 gaactggcat gagcggatcc gttcgcgccc tgccaccggg caggcactgc taaaagcaca
    32281 actcggtgat gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt atttcatcct
    32341 aagtaaaaca acggagaacc tgcaatggca caacctgccg ctattattcg tataaagaac
    32401 cttcgtttgc gtacgtttat cggaattaag gaagaagaaa ttaacaaccg tcaggatatt
    32461 gttatcaatg tgacgatcca ctaccccgcc gataaagcgc gcactagcga agatatcaac
    32521 gatgcgctga attatcgcac cgtaacgaaa aacattattc agcatgtaga gaataaccgt
    32581 ttctctttgc tggaaaaatt aactcaggat gtgctcgata tcgcacgtga acatcactgg
    32641 gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta
    32701 tccatgacct taagctggca gcgttaatcg ccatatcggg aggctgcatg aacatagtga
    32761 tcaccggagg gacgggatta attggtcgcc atttgattcc gcgtttgctg gagctgggcc
    32821 atcaaattac ggtagtgacg cgtaacccgc agaaagccag ttccgttctc ggccctcggg
    32881 tgacactatg gcaagggctt gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca
    32941 acctggccgg agaaccgatt gctgataaac gctggactca caagcaaaaa gagcgtctct
    33001 gccaaagccg ctggaatatc acgcaaaaac tggtcgattt gattaatgct agcgacacgc
    33061 cgccgtcggt actcatttcc ggctcggcaa cgggctatta tggcgactta ggtgaagtgg
    33121 tggttaccga agaggaaccg ccgcataacg aatttactca taaactctgc gcccgctggg
    33181 aagagattgc ctgccgggcg caaagtgaca aaacgcgagt gtgcctgctg cgaaccggcg
    33241 tggtactggc accggacggc ggtattctcg gtaaaatgct gccaccgttt cgtcttggcc
    33301 tgggcgggcc gattggttcc ggtcggcagt atctggcctg gattcatatt gatgatatgg
    33361 tcaacggcat tctctggctg ctggataacg agctgcgcgg gccatttaat atggtttcgc
    33421 cctacccggt acgcaatgaa caatttgccc atgcgctcgg tcatgcgctg catcgcccgg
    33481 ctattttgcg cgtccccaca accgccattc ggctgttaat gggcgaatct tcagtactgg
    33541 tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt gcgtttcgct
    33601 ggtacgattt agaagaggcg ctggcggatg tcgttcgctg atgtggttta cagcaaacat
    33661 ccgccagtta actccctgtg ttacaggatt agtggctttg cgcgataaga tcgtctggtg
    33721 aaagtcgggt caccatcata actaactctc tgtctaaacc tctatccagc atctcctgag
    33781 caatacgcag ggcttcttcg tgtttgccct gcattgcgcc ttcttcacgt aatctgtcag
    33841 caatggtcat cagtttctcc ttttcttgtg gtgcgcgttc tgttatctca ccaataaatg
    33901 cacggaaacg ctgggcatcc cctgtttgta atacgtaatt aaacagggct tttagctgtc
    33961 tgtcattagt gttccctgta actaacagcg aaacaatttg gtcgactaat cccaacagat
    34021 cgcgctgacg aatatgtttc tgaattaact ccaacagcgc cattttgcgg tgttgcataa
    34081 tctcgtcatc cggcaccacg gtaatatcca ccaacggaaa cgctgatgaa tatattttgc
    34141 gggctatagc aggctcggca aattcatcaa gccagcagag tgaataagga taaggacttc
    34201 tgcaaccatg ataaaacagc atcgggatca ccaatggaag ctctttatag cccgcatcaa
    34261 gatggttttg cattgccgca atggaataac gcatcatgcg aaaagccatt aattcttccg
    34321 gcttactttg gtgctctatc actacataaa tataacccac tccctcctgc gttttcacag
    34381 accacaagag gtcggaataa tattgccgca ggtcttcatc aataaaactg tttggttcta
    34441 gtttaagcgt cgttaaatca cacagtttgc gcagcggcgc gggaagatga atattaataa
    34501 aattccgcgc ggtgtctgga tggcgtaaaa aagatttaaa taccgcatca tgcggcgtgg
    34561 aagttgtcga tattgtcatg gcgttccgtc acccctcaaa tttaagatga cgcgacaata
    34621 accacgatta acacccaatg cagcaggcag tttttatctt tggacagcga attcagaagt
    34681 atttttagcg gactgcaact tttacatttc agcggaagct gactcgcaaa ttccccttga
    34741 aaatactccg gcagcgttag ccaccggaga ggaaattatt tcagcgatcc cttaaggaac
    34801 tgttgcagac gagggctttg cgggttgcca aataactgct ccggcgcgcc ctcttcttct
    34861 attttcccct gatggaggaa aatgacgtga gtagaaacat ggcgagcaaa gcccatttcg
    34921 tgagtcacta ccaccatggt tttcccctct tctgccagtt gctgcataat acgtaacact
    34981 tcgcctacca gttcaggatc gagcgccgag gtaggttcat caaacagtaa aacttctggt
    35041 tccatcgcca gcgcccgcgc gatagaaaca cgctgttgct gaccgccgga aagatgcacc
    35101 ggatatttcc cctgcgcacg ttcgtctatc ccgactttcg ccagatactt caccgcccgc
    35161 tcgcgcgctt cctgcttgct caggcccaac acctgaatcg gcgcttccat gacgttttcc
    35221 aacaccgtca tatggctcca gagattgaag tgctggaata ccatcgtcag gcgtgtgcgc
    35281 agtaagcgca gttgattttt atcggcgact ttgagttgac cgtctttgtc gcgcaccaga
    35341 ttgatcgtct ggccattgac cacgatcgat ccttcactcg gtttttcgag gaagttaatg
    35401 cagcgcagaa aggtactttt ccccgatccc gacgatccga tgatgcttat tacatctccg
    35461 gcattcgctt gcagtgagac ccctttcagc acttcgtgtt cgccatagcg tttgtgcaaa
    35521 tcgataacgt ttaatttatt ctcggacatc gtattctcag tgcgttgaag aaggtttcac
    35581 atgctgcaac cagcgttttt ccgctctgcg aaagaggctg atcaggacat aagagatgat
    35641 taaatagagc accgcggcaa tgccgaaggc ggtaaaaggt tgatacgtgg cggcgttaat
    35701 atcgcgggct attttcagca gatccggcac agtggccgta aacgccagcg cagtagagtg
    35761 cagcatcagg atcacttcgt tgctgtatgc cggtaacgca atacgcagcg cagaaggcaa
    35821 aataatgcag cgatacattt taaaggtcga gaagccatag gctctggcgg cttcaatttc
    35881 cccatgcgga accgaacgga ttgccccagc aaaaatctcg gtagtgtaag cgcaggtgtt
    35941 aagcgtcagc gccagcacgg tacagttcag gccactgcgg aagaaagcgt taaggaattc
    36001 ggttccctta acaatctcaa gcgtgtacat gccggaatag aacaccagca actgaacata
    36061 cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt ggaaactgga tgtatttatt
    36121 actggagaca cgaccaatcg ccagaaacag cgccaggact ccgcctatca ctaccgacaa
    36181 aataagcagc cacagagtga tcgccacacc agtaaagcga taaccgtcgg tccacagcag
    36241 cggtttccag tattcatgta agatttcgat cacaggtcag ccctcttcac acccacggag
    36301 tagcggcgct caaggaacag cagcacacca ttggaaacgg tggtgaaaac caggtaaatc
    36361 acgccacaga cgatggcgaa atagaacggt tcccaggtac ttttgcctgc cagctgcgtg
    36421 gctttgacca catcttccag gccgagtaac gaaaccagtg cggtagattt gaggatcacc
    36481 tgccagttgt tgccaatgcc tggcagggcg taacgcatca tcgccggaaa catgatccgc
    36541 cgaaacactt gcccgcgagt aaaaccgaac gccgtcgccg cctctatatg ccctttcggc
    36601 actgccataa acgcgccacg gaacgtttcg gtaaaataag caccgtagat aaaaccgaga
    36661 gtgataatac cagcgaccat cggatcgata tcaatctgcc cgacgcccat cgcctccgtc
    36721 accatgttta gcgcaatctg caaaccatag aaaatcagca gcatcaacac caagtccggc
    36781 acgcctcgaa tcagcgtggt gtacccttcg aaaatcagcc ccgaaagccg attttgcgag
    36841 agcttaccgc cagcgccaat taaaccgatg attacagcga gcactacaga gctgatagcc
    36901 agctccagcg tgacgagcgc accctgtaaa ataacacctg aaaacccata caacatgctg
    36961 cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc cctccggtta cccgaaggga
    37021 gcgggctttc tttcaccgat ggcgattaac caccataaac atcaaaatcg aagtactttt
    37081 tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc aaaggctttg ttcagtgcct
    37141 cgcgcagttc gttatcttct ttacgcaggc ccatgccggt ccctacgcca aacagttttt
    37201 catctttaac agacggacca ccgaatttgt aatctttacc gacgggttgt ttgaggaaac
    37261 cttcgctggc agcgacctca tcctggaacg cggcatcaat acgtccggca gtcaggtcag
    37321 aataaatgtt gtcctgcccc tgatacgaga cgatttcaat gccttttggt gcccagtgtt
    37381 cattaccgaa cgtctcctgg gtggtgccct gcaatacgcc tacccgtttg cctttcagcg
    37441 actcgactgt cggctgaatg tcagaatttt tcgccaccac cagacgagaa tcggcagcgt
    37501 acagtttgtc ggtgaaggca atttcttgct gacgtttttc cgtaatggaa agcgatgaca
    37561 tgatggcgtc aatcttcttc gcttttaagg acgggattaa cgcatccagc gggttttcga
    37621 caaacgtaca ttgcgtattg atgcgcttgc acaactcctt cgccagctcg atatcgaaac
    37681 caaccagttc gccttgtgaa ttttttgatt caaatggcgc ataggtcggg tcggtaccga
    37741 tgcggatgtt ttgcggaatt gcagcaagtg ccgcagttgc gctggagaag gccagaacca
    37801 gagagagcga tagcaccagt tttttcataa tgtcctcaac tgacagtctt tttataggga
    37861 atatttacag gtttgttaca cttatcgtgc cataaaattg accatcaagg caaaatatcc
    37921 cgccccgtta gacgtattat ttttcattaa aaatgcttaa gtatgcattt caatgcacca
    37981 cgatagtgca acaaacctgg cgtgcaccat gatgatcatt ccatcaggta cagcttccca
    38041 gcgacgtatc agtcaccgta gacattaaag tcgaaatact ttttcgccat cttgtcgtag
    38101 gtgccgtcct gacgcagctc gccaagcgcc ttattgaagg cagccgtcag ttcagcataa
    38161 tctttacgta gccctacacc ggtgccatca ccgaagtatt ttttgtcttt tactgatgag
    38221 ccagcaaaag cgaaatcttt accagcaggt tgcttgagga atccttcgct ggcagcaact
    38281 tcatcttgta acgcagcatc cagacgtcct gcagccagat cggaatagac caaatcctgg
    38341 ttggcatagg ccaccacatc cacgccttta ctacgccagg tctcgttagc gtaagcttcc
    38401 tgggttgatc cctgcagcac accaacatgt ttacctttca gtgaatccag cgttggctga
    38461 atcggtgaac ctttggcagc aatcaaacga gaatctgcgg cgtacagctt gtcggagaag
    38521 gcaatctcct gctgacgttt atcggtaatg gaaagcgacg aaataatagc gtcgattttt
    38581 ttcgctttca gtgaggggat cagcgcgtca aagtcactgg caacccaggt acatttcacc
    38641 tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa agccaacaaa atcaccttta
    38701 gcatctttcg acgagaacag cgcataggtg gtatcggttc cgatacgtac cgtctccggt
    38761 agcgccgcat agctggaagc cgctgcggag agaccgacta ataaagacag agcgagaatc
    38821 gacttcttca tacataaccc tcaagtgaaa atggcttttt tatgttgtgt attgtgttgt
    38881 gtgtttgcag gctctttcat gcaggtctta tgccatcttg acgacaacag taacattcaa
    38941 cgttaaatat gttaataaga cattgcatta ttgtcctgaa gttgaagata gcaggtatag
    39001 cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa atgtcgagga tttgatcgcg
    39061 gttgcaaaat tgccctgaaa cagggcaaca gcggagttat gcgccctgcc agcgggcaaa
    39121 gagatcttca ggaagggtta ttgcaaactg gtcaagaaca cgattaaccg tctgatttat
    39181 cacatcatca agggattgcg ggcgatgata aaacgccgga acgggaggca taatcaccgc
    39241 accgatttct gccgcctgag tcattaaacg cagatggcct aagtgcaatg gtgtttcacg
    39301 cacgcagagc accaacgggc gacgctcttt cagcaccaca tctgccgcac gggtcagtaa
    39361 gccatcagta tagctatgga caatgccgga cagggttttg attgaacagg gtaaaatcac
    39421 catccccagc gtctggaaag aaccggaaga gatgctggcg gaaatatcgc gcgcatcgag
    39481 cgtgacatca gctaatgcct gcacttcgcg cagggaaaaa tccgtttcga gggataaggt
    39541 ctggcgcgcc gcctggctca tcaccagatg cgtttcgata tctgtgacat cgcgcagaac
    39601 ctgtaataag cgcacgccat aaatcgcgcc gctggcaccg ctgatgccta caatgagtcg
    39661 tttcataaat agttgccctg tcagacttgc aggcagactt tgcagtattt cggcgggagt
    39721 tgcaagtcag ggtgccagac cggcaccctc agcgaaggca tcatccttcg ttatgcattt
    39781 cgagattttc cacttcgttc tgacgttgca ctgctttggc gtcatcatta cgtaacgtat
    39841 cgaggaaatc gaggtagccc tgatcaacat ctttggtgac gtagacgccg ttgaacaccg
    39901 agcattcaaa ctgctggata tccggatttt cagcgcgaac ggcgtcgatc agatcgttca
    39961 gatcctggaa aatcaacccg tcagcaccga tgatctggcg aatttcatca acttcgcgac
    40021 cgtgagcgat cagttccgtg gcgctcggca tatcaatacc ataaacgttc gggaagcgaa
    40081 tttccggtgc cgcagaagcg aggtacactt tcttcgctcc ggcttcgcgt gccatctcga
    40141 taatctgctc agaagtggtg ccacggacga tggagtcgtc gaccagcagg acgtttttat
    40201 cgcggaactc ggcgcggttg gcattcagtt tacggcgcac ggacttacga cgcagctgct
    40261 ggcccggcat gataaaggtg cggccaacat agcggttttt aacgaagccc tggcggtacg
    40321 gtttgcccag aatacgagca atttccagcg cgatatcaca cgaggtttct gggatcggga
    40381 tcaccacgtc gatatccaga tcttcccatt cgcgggcaat tttctcgccc agtttcgtgc
    40441 ccatattcac acgcgcgctg taaacggaaa ttttgtcgat aaacgagtcc gggcgggcaa
    40501 agtatacata ctcaaacagg cacggattgc tgaccggatt gtcagcacat tgacgggtaa
    40561 acaactgccc ttcttcagtg atgtaaatcg cttcgcccgg cgcgacgtca cgcaggaaat
    40621 caaagcccag cgtatcgagc gctacgcttt cggaagcgac catatattct gtacggttct
    40681 cgtcaatatc acgttttccc agtaccagcg gacgaatccc gtttggatcg cggaaagcaa
    40741 ccataccgtg gccgataatc atcgccacac aggcatacgc gccgcggatt aagcggtttg
    40801 tggcagcaat ggcagcgaaa atattgtcgg cttccagcgg gtagtggcgg aagttgtcca
    40861 gctcgctggc gaagatatta agcagaattt ccgagtcgga agtggtgttg atgtggcggc
    40921 gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt ggtcagattg ccgttgtggg
    40981 caagcgtaat gccatacggg gagttaacgt aaaacggctg cgcttcagag gcgctggagc
    41041 tgccagccgt agggtaacgc acatgaccaa tgcccatatt gccctgcaaa cgctgcatat
    41101 ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt acgcaaacgg aagcagttat
    41161 tggcatctat ggtgatgatg ccggcggcat cctgaccgcg atgctgaagc accgttaagg
    41221 catcataaat cgactggtta accggcataa caccggcgat accgacaata ccgcacatac
    41281 gtctttttcc tcgttaagcc acatctcaga gcacttacgc tctgggcaag aaacttgacg
    41341 agctttgcag ataatcaaaa aaccatctga tgataaaact gaattgcggg atcagctgtg
    41401 atttgctcca gtcttcactt ttcgacaccc cggtaaagga gtcgagaaag aagagaatgg
    41461 cggcaacaat caacacaccg cgcaacgcac cgaaacagac gcccagcacc cgatcggtgc
    41521 ctgacaaccc cgttttctcc accaactggc ctatcacgaa gttcacgata gcaccaacga
    41581 tcagggtagc gataaacagt accgcgatgg caatcccatt tcgaaccagt tcgtcttcaa
    41641 agcccgtaaa ccagactgac aggtaagtgt agtaatgact ggcaacaaag aaagcacaac
    41701 cccatgtcac cagcgataac gcttcacgaa caaagccgtg gatcaggcta accagagagg
    41761 aaaaagcaat caccgcgatt atggcgtaat caatccagac catatgtgtc ccacgatttt
    41821 acgccctgtc atcctgttcg gggcgcattc taacagaaaa agaaaacgtt tgcgtaggga
    41881 tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat attcaacgcc atcgactttt
    41941 tatgcctttg cggcatcgtg caatgcgtgt cggatgcggc gtaaacgcct tatccgacct
    42001 acggttctaa cactgcccaa gcctgataag acgcgccagc gtcgcatcag gcaagactgt
    42061 attaattcgg cgtatagccc attaccacgc cacttaagcc agaaagttgc ttcaactcac
    42121 ccagcgaacc tttcagctta tctttcgagg catccggccc aaccagaata cgggtaattt
    42181 taccctgcac tggcgtggat ggcgacgtat aaacccgata accggcaccg cgcagcttac
    42241 cgacaatctc attcacttta tcggcatttt tcagcgcacc cagttgcaca acataggctt
    42301 tacccgttgg cgctgctttt tcttccacga ccggcttcgg ttctggcttc ggcgcaggtg
    42361 gtacttcaac ctttggttta ggcggctcca ccggtttcgg cttcggtggg gcgaccggtg
    42421 caggttccgg ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg agcgacggtg
    42481 ctgcggcatc acctgcccgc acctcttccg ctgcgccttc cggcggctgc gtcggcaacg
    42541 cctgggtggc ggctggcatc atatcaggct catcacgatc gcccgctttc ggcaccagcg
    42601 ggatagccgc gaactcatcc tgataatgtt ttttctgccc gtccagcagc cctggaagta
    42661 caatcacccc cagcgccacc agcacgatcg tgcccactaa ccgattctga aacttacttg
    42721 ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg gaaagaacca
    42781 cacaccagca cggtgtcttc cgctttagcg tccgccattg cggcatccca tgcctgcgca
    42841 acgctatcaa atgatttgcc gttacccaaa tgctcaagca gttgttctgc cgtggcaccg
    42901 cgcggccctt ccagtggcgc acaataccag tcatcaacca cgcttttcaa ccaggccaga
    42961 gttccggcaa tatctttatc atgtagcata ccgataaccg ccagcacgcg cccgtttttc
    43021 ggtagcgctt tcatacgccc ggtgagatat tccgccgcat gtggattatg cgcgacatca
    43081 aaaataacgc gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca
    43141 atcccgtcgc gaatggcatt ttcactgact tccagcccgc tggcacgcag tgccgccagc
    43201 gccgttgcgg cattcggttg cgggacaagc ggcaacggca gattttccag cgtgccgtga
    43261 gcatcgctaa acgcccagtc atgatcggtg acggaatagt tccactcaac gccccgacgt
    43321 tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg tagaaggcat ttccggctca
    43381 ccgacaattg ccggtttttc gctgcggaag atgcctgctt tctcgagacc aatactttcg
    43441 cgatctggac ccagccagtc ggtatgatcc agcgcaatac tggttactac cgcgacatcg
    43501 gcatcgacaa tattggttgc gtccagacga ccgcccagcc ctacttccag aatcaccacg
    43561 tcaagttgtg cctgcttgaa cagccacaac gccgacagcg taccgtactc gaaataggtc
    43621 agggaaatat caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc
    43681 ggcaattcct ggccctgcac acgtacgcgc tcggtataac gcaccagatg aggcgaactg
    43741 tagacgccca ctttgtaccc tgccgccatc agaatcgact ccagcgtacg gcaggtggtg
    43801 cctttgccat tcgtacccgc aacggtaaac acaaatggcg ctggtttcag gacgccaaga
    43861 cgcgccgcga cctggctcac gcgctcaagg ccgagatcga tagttttact gtgcaggttt
    43921 tccagataag aaagccacga agccagaggc gacgcggctt gaggagtgcg tttgataatc
    43981 atggtatccg ctgattcgtt acggtgagaa tagcaaaagg gcagagccag tggccctgcc
    44041 cttatcagtt atcaggcctc aggttcctga tccggtaccg ggggtaccac tacgccttca
    44101 cgcggcgctt caggattcgg cgctggcaga ttcatcaact tcgccagaat gctcgccagt
    44161 ttcaggcgca tttccggacg acggacgatc atgtcgatcg cgcctttctc gatcaggaat
    44221 tcactgcgct ggaatctagg cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga
    44281 ccggcaaagc cgattaacgc tttaggttca gcgatgttga gatcgcccag catggcgaaa
    44341 cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg caagccgcgc
    44401 tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga catcagtgct
    44461 tcctgcatac gtgcgccacc agaggcggag aagcagatca gcgggcagtt atcttcctgc
    44521 gcctgctcaa cggcacgcac gaaacgtgca cccacaacag accccattga accgcccata
    44581 aaggcgaact cgaatgccgc agcgacaacc ggcattccat acagagtgcc tttcatcacc
    44641 accagcgcat ctttttcgcc ggtttctttc tgcgcagatg ccagacggtc tttatacttc
    44701 ttggagtcac gaaacttcag ctcatctttc ggctcaagct cgctacccag ctccacaagg
    44761 cttccttcat ctaacaggct atgcaggcga ttacgcgctg tcatacgcat gtgatggtca
    44821 cacttcggac agacctcaag attacgttcc agctcagcgc ggtataaaac ctgaccgcag
    44881 ctatcacact tagtccacac cccttcagga atgctcgcct tgcgggtggg agtaatgttg
    44941 cttttaattc gttcaatcca gctcattagg gacctttctg tctgaacctg gttcgatgcc
    45001 agttttatct ttggggacgc ataatgccat ttttgccccc aacagaccat gaatgttgca
    45061 cattaaaaca taacagcccg aaactttgga taaaaaagtg gtcgaaccgc ggagttactt
    45121 tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacggat tatttcgatg acgcccggca
    45181 aaatagaaac cacaataatc ccgacgatca gcagcttaag gttatcctga accatcggga
    45241 ttgtaccgaa gaaatagcct gcgtaggtaa aaagcagtac ccacaacagt gcgccgatca
    45301 cgttataagc agcgaaatga cggtacgaca tgtggcccat tcccgcaaca aacggggcga
    45361 acgttctgac gatcggcaca aaacgggcga gaataatcgt tttgccgcca tgtttctcat
    45421 aaaactgatg ggttttgtcg agataactgc gacggaaaat tttcgaattg ggattactga
    45481 ataacttttc accgaacaac cgcccgatag tgtaattgac cgcgtcaccc acaatcgcgg
    45541 cgatcagcat cagcactacc atcatatgga cgttgagatc gttggtttcc agtgatgcca
    45601 acgctccggc aacaaacagc aatgaatcac ccggtaaaaa cggcattacc accagaccgg
    45661 tttcacagaa caaaattaag aacaaaatgg cataaaccca gacgccgtac tccgcgacta
    45721 gctctgccag atgcacgtca atgtgcagga taaaatcgat gaggaaataa atcaggtcca
    45781 tatttgccta tgccttgtac tcgtcatctt tcaggctgta actacgttgg ctacagcctg
    45841 aaatgttccg agcattattc tcgttagtcc gccagaaata gcgggcccat tggcggtttt
    45901 ggaagatcat accggtcagg gtaatccacc gcgaccagat acagcccttc cgcttttgcc
    45961 gttgctgccg ccagcgttct gtcctttgcc gccagcaact ctgctatcca gctctccggc
    46021 tggttgtggg caccgacttc catcaggctg ccgacaatat tcctgaccat atgatgtaca
    46081 aaggcattcg ctttgatatc aaccaccaca taaggaccgt gacgcgtgac attaatatgc
    46141 ataacgttgc gccacggggt tcgggactgg cactgcaccg cacggaacga ggtgaaatca
    46201 ttctcgccca gcaagcattg cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa
    46261 aaatgggtta cccctttact cagtaccgcc gggcgcagcc gatgattgta gatgatgtag
    46321 cgataacggc gagccgtggc gctaaatcgg gcatgaaaat catcaggtac agctttaacc
    46381 caacgcacgg cgatgtcacc aggtaaattc gcatttacgc ccaaggtcca cgccgcgtct
    46441 ttgcgctgcg cggttgtttc gaaatgcacc acttgcccgg taccgtgtac ccctgcgtca
    46501 gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca cctgggagag cgccttttcc
    46561 agcttctcct gcacactgcg gacttcattc tgccgttgcc agccgtaata cttactgccg
    46621 tcgtactcaa tgcccagcgc aattttataa actggcagtt gttgctggtc ggacattagt
    46681 acagatactc ctgcaccagt ttctcggcga tttttactgc catcagcgcg ccgccaaagc
    46741 gaacgttatc ggccaccgac cagaactgga cttgctccgg cataccgtag tcattacgca
    46801 cgcagccaac agaaagatgc ggcgtacccg aagcatcccc tacctgagtc gggaattcgt
    46861 tctcttcaga gagcacaata tcttcgccct gagcaaacgc atcacgcgct tcttctgccg
    46921 ccagcggacg cagtgcttca aagttgacca tctgggcatg accgtagaat accggtgcct
    46981 ggacgacgct tgccgaaatc atcagccctt cgtcctgcag gattttgcgt acttcgtcaa
    47041 cgatacgacg ttcttcacgc acgctacctt cgctatccgg caacaacggc agcatattga
    47101 acgccagttg acgaccgaag aaatcttctt cgtcaatcgg aatgccgttg agcaatttcg
    47161 cactctgccc cgctaacgca tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc
    47221 tggtaacact gatacgcgac aaaccgccct gatcgattaa cggtttcagt gccgccagca
    47281 gctggctggt cagactgtct ggtacggcga tgacattccg gttgcggtaa tcggtcagta
    47341 caaacgggtt tacttccggc accaccagcg gtacgtcggg ttcgagagca aacagtccgc
    47401 tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc aacccaggca gcggtagctt
    47461 ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca gcggcatcct
    47521 gcacggtgat tgttttacca ccaaagcgca gttgttcgcc tgcgctttcg ttacgtgcca
    47581 gtgcataaat ttccccaacc gggaactgac gttcagccag cgtttcaagc agggcttcgc
    47641 ccatagcgcc agttgcgccc aggacggcaa tgttccagcc ttcagacatg gtggtttact
    47701 ccagaaatag caaagctccc tgccaatgtt acagcagaga gcatgaagaa gagattaacg
    47761 tgccggatga tgaacggcgt taaaacccag tttacacagc aatgatgccg cactggcgtc
    47821 atcacaaatt acatacagag atgaccattc acggcgctca agatagtttt tgcgcagttt
    47881 atcgaactca cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc ggcgcacatc
    47941 atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg agcggaccat gcagcgtaat
    48001 gcggccaaac tcaggcgcgg gcagcaacgt atccagcgca acgtgttgtt catgcccaat
    48061 aaacttgcta taggcttcaa acacttgcgt cgtgccacgc gctttaccct ccagggtata
    48121 gcctgcgata tgcggcgtgc cgatatccac ttttgtcagc aattccacat taagttccgg
    48181 ctcgccttcc cagacatcca gcaccacgct taacttctgg ccttcattca ggcaggtcag
    48241 cagcgcggta ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg ctccgggctt
    48301 caggctacgg atcagttttt catccgccag atgtagcgtt ttgtacggac catctttaaa
    48361 gagtggcgta tggaaagtca gaatatccgc gcgctggact aactcatcca gcgagcggaa
    48421 atcaccctca tccccacggt cggcgcgagg cggatcgcaa agtaaggttt taatccctaa
    48481 cgcttccagt cgcgcctgca atcgacgtcc aacgttacca acgcccacga tccccaccgt
    48541 acggtcgtgc agtgaaaatc catcgcgttc ggcaagcatc agcagggagg agaaaacata
    48601 ttccaccacc gcaatcgcat tacagccagg tgcagcggaa aaaccaattc ccgcctgctt
    48661 caaccatgct tcatcgacat ggtcggtccc cgcagtggca gttccaacaa atttaatggg
    48721 ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc
    48781 gtctgccagt tgagcgacgg ggattggacg cccgggaacc gcgatcacct cacccaaacg
    48841 gctaaataag tcgcgggcat aaggcatatt ttcatcaaca aggattttca cgtttgtgtt
    48901 acctgtatga gacgagagtt aaccggacaa gtatgccata atctcgcggc caggcatact
    48961 tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc tatttccggc cctcctcctg
    49021 ggcagccacc aggtcaggga gataacctgc cgtctggcgc gggcaatcag cctttatcca
    49081 gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt gacctcactg acgcaacaac
    49141 aaagagcaga actgtgggcg ggtatcaggc acgatattgg tctgtcggga gattcaccgc
    49201 tgctttcgcg tcacttccct gccgctgagc ataatctggc gcaacgtctg ctggccgcgc
    49261 aaaaaagcca ttctgcccgc cagcttttag cgcaattagg ggagtattta cgtctgggga
    49321 atcatcgtca ggcggtcacg gattatatcc gtcataactt tggtcagacg ccgctgaatc
    49381 agctctcacc ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg aagatggtta
    49441 ttccgcaacc acagcagcgc gaggcgaccg accgtccttt attaccggcg gagcacaatg
    49501 cgctaaaaca gctggtgacc aaacttgcgg cggcaacggg ggaacccagc aaacagatct
    49561 ggcaatcgat gctggaactt tccggggtga aagatggcga gttaattcca gcgaaactgt
    49621 ttaaccatct ggtgacctgg ctacaggcgc gtcagacgct aagccagcaa aatacgccga
    49681 cgctggaatc actacagatg gcgctaaaac aacctttaga tgccagtgaa ctggcggcgt
    49741 tatcggcata tatccagcaa aaatatggcc tttctgcgca atcatcgctt tcttctgccc
    49801 aggccgagga tattcttaat cagctttatc aacggcgggt taaagggatt gatccgcgtg
    49861 atatgcaacc gctgcttaat ccttttccac cgatgatgga cacgttgcaa aatatggcaa
    49921 cgcgtcccgc gctgtggata ctgttagtcg cgattatcct gatgctggtc tggctggttc
    49981 gttaaccccg acgaaatgac agtatcgtga caataatacc cagcaccgca gagatcgccc
    50041 cggcaagaaa taccgaagag taaccaaacg tggtcgccag cattcccgcc agcggcccgg
    50101 agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaactt
    50161 gtgaggggac gcgtttaacc acctccacgc ccagcgcagg aaagataagc gaacatccgg
    50221 ctccggttaa cgccgcgccc gctaatgcga cccatgcacc tggggcttgc cagagcagca
    50281 acaagcccac cgtttctaca agcagagaga caatcgccac tttcacgccg ccaaaacggt
    50341 ccggcatcca gccaaacatg acgcgcatca cgacaaatgc gccgccaaac gcggtaagag
    50401 taaagcccgc catcgcccat cctttgctgg caaagtagag cgaaacgaaa gtcccgataa
    50461 ccgcaaaacc aacgccttgt agtgccagcc ctaaccctgg tttccagata agcccgacaa
    50521 cgctccacag cgatggacgt tctcccgcca gggccggtac tttgcgcact gtgccgttac
    50581 aggcccacgc cagtaagggt aataccattg tggtgatcgc cagtgcggca aaaccgtaat
    50641 ggctatgaat caacaggcca agcggcgcac caacagcgag ggcaccgtaa atcgccattc
    50701 cgttccatga catcactttg ccagagtgtt ttggccctac gatgcctaac ccccaggtca
    50761 gagcgcctgt cagtaactgg ctttcaccaa acccaagaat caaacgcccg atgaccaaca
    50821 gggcaaattt gaacggtgcg gagacaggca aaatcgccgc cagcagcaac gcgccgccag
    50881 ccagaccaca agctaacatc ccctgaagcg ccgaacgttt tgcgccatat tgatcggcca
    50941 gtcgcccggc gtaaccacgc gtcagcaccg tagccagaaa ctgaatcccg acggcaatgc
    51001 cgaccatggt attgccatag cccagatcat gatgaacaaa cagcgggata accggcaacg
    51061 gcaaccctac ggtcatgtag gtgagaaaaa ccgcaaaaga gatgcggaag agcgaaaaac
    51121 tggcagaaga tcgtgtttcg gtttggctta cagcagtcat gcattactcc agaatgcagc
    51181 gcaaggcgag gagtatcccc gtctcatctc tctggtttca gggttacggt gcgttggcag
    51241 gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt gcctagaact
    51301 ggtggcgatt gtcaatgatg tgaaaaaggg aaccatcagg ttcccttttg cgttagtgcc
    51361 ggaggccgca ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc cgacctactc
    51421 cggcctactg cgaattaatc ttttcagctt gcgcattacc agcgtggcgt tggtgccgcc
    51481 gaagccgaag ctgttagaca taacagtggt cagttcgcga tcggtcgttt cggtcacgat
    51541 gttcagaccc gcagcctgct cgtccagctc ttcaatgttg atgctcgggg cgataaagcc
    51601 gtgttccagc atcagcagag agtagatagc ttcctgcaca cccgcagcgc ccagtgagtg
    51661 accggtcatg gctttggttg cagaaatcgc cgggctctta tcgccgaaca cttcacggat
    51721 agctgccagc tctttcacgt cgccaaccgg agtcgaagta ccgtgagagt tcaggtaatc
    51781 gattggggta tcaacgccat gcatcgccat ctgcatacaa cgtactgcgc cttcgccaga
    51841 cggagcaacc atgtctgcac catcagaggt tgcgccgtag ccaacgattt cagcatagat
    51901 gtaagcacca cgcgccagcg cgtgttccag ctcttcaacc actaccatac cgccgccgcc
    51961 agcgataacg aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc
    52021 gttgtattta gtagatagcg cacccatcgc gtcgaattcg caggccattt cccagcacag
    52081 ctcttcgccg ccgccagcaa acacgatgtc ctgtttgccc agttggatct gctctactgc
    52141 gttaccgata cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt taacgccatg
    52201 aattttaaac ggcgtggcga gacaggcaga aacgccggat gccatcgctt tagtgaccac
    52261 atacgggcca accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc cgaacacctg
    52321 gaaacgcggg gaaccgccgc cggaacctgc aatcaggcca acgcgcgggt tattctggta
    52381 agcttccgga gagaggcccg catcagcgat tgcctgctcc atagaaagga atgcataaat
    52441 ggatgcgtcg ctcataaagc gcacaacttt gcggtcaatg aggccagtgg tatccagttt
    52501 tatgttgccc cagacgtggc tacgcatgcc ggaatccttc agctcctgag agaaagtgat
    52561 ccctgaacgt ccttcacgca gagatgccag gacttcctgc tggttattac cgatgctgga
    52621 aacaatgccc aggccagtaa tcactgcacg tttcattcaa tacctctgta agtcgcacat
    52681 agagtaagtt tcgaatgcac aatagcgtac acttgtacgc cgaacaagtc cgatcagcca
    52741 tttaatagag atatttgcgc agccttacac acatcgctaa gatcgagcca ccgcctgtaa
    52801 gacgagtaac ttacgtgaaa cactactcca tacaacctgc caacctcgaa tttaatgctg
    52861 agggtacacc tgtttcccga gattttgacg atgtctattt ttccaacgat aacgggctgg
    52921 aagagacgcg ttatgttttt ctgggaggta accaattaga ggtacgcttt cctgagcatc
    52981 cacatcctct gtttgtgata gcagagagcg gcttcggcac cggattaaac ttcctgacgc
    53041 tatggaaggc atttgatcag tttcgcgaag cgcatccgca agcgcaatta caacgcttac
    53101 atttcattag ttttgagaaa tttcccctca cccgtgcgga tttagcctta gcgcatcaac
    53161 actggccgga actggctccg tgggcagaac aacttcaggc gcagtggcca atgcccttgc
    53221 ccggttgcca tcgtttattg ctcgatgaaa gccgcgtgac gctggattta tggtttggcg
    53281 atattaacga actgaccagc caactggacg attcgctaaa tcaaaaagta gatgcctggt
    53341 ttctggacgg ctttgcgcca gctaaaaacc cggatatgtg gacgcaaaat ctgtttaacg
    53401 ccatggcaag gttggcgcgt ccaggcggca cgctggcgac atttacgtct gccggttttg
    53461 tccgccgcgg tttgcaggaa gccggattca cgatgcaaaa acgtaagggc tttgggcgca
    53521 aacgggaaat gctttgtggg gtgatggaac agacattacc actcccctgc tccacgccgt
    53581 ggtttaatcg tacgggcagc agcaaacggg aagtagcgat tatcggcggt ggtattgcca
    53641 gcgcgttgtt gtcgctggcg ttattacggc gcggctggca ggtagcgctt tattgcgcgg
    53701 atgaagcccc cgcgctgggt gcttccggca atcgccaggg ggcgctgtat ccgttattaa
    53761 gcaaacacga tgaggcgcta aaccgctttt tctctaatgc gtttactttt gctcgtcggt
    53821 tttacgacca attacccgtt aaatttgatc atgactggtg cggcgtcacg cagttaggct
    53881 gggatgagaa aagccagcat aaaatcgcac agatgttgtc aatggattta cccgcagaac
    53941 tggctgtagc cgttgaggca aatgcggttg aacaaattac gggcgttgcg acaaattgca
    54001 gcggcattac ttatccgcaa ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc
    54061 tggaactggc gcaacagcag ggtttgcaga tttattatca atatcagtta cagaatttat
    54121 cccgtaagga tgactgttgg ttgttgaatt ttgcaggaga tcagcaagca acacacagcg
    54181 tagtggtact ggcgaacggg catcaaatca gccgtttcag ccaaacgtcg actctcccgg
    54241 tgtattcggt tgccgggcag gtcagccata ttccgacaac gccggaactg gcagagctga
    54301 agcaggtgct gtgctatgac ggttatctca cgccacaaaa tccggcgaat caacatcatt
    54361 gtattggtgc cagttatcat cgcggcagcg aagatacggc gtacagtgag gacgatcagc
    54421 agcagaatcg ccagcggttg attgattgtt tcccgcaggc acagtgggca aaagaggttg
    54481 atgtcagtga taaagaggcg cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa
    54541 tggtaggcaa tgttcccgat tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc
    54601 aaaaagatga ggcggtaagc gcgccggttt ttgacgatct ctttatgttt gcggctttag
    54661 gttctcgcgg tttgtgttct gccccgctgt gtgccgagat tctggcggcg cagatgagcg
    54721 acgaaccgat tccgatggat gccagtacgc tggcggcgtt aaacccgaat cggttatggg
    54781 tgcggaaatt gttgaagggt aaagcggtta aggcggggta atctgctctc gcagtgtttg
    54841 ccggatgcgg cgtgaacgcc ttatccaacc tacgtgtgac gtgtaggcct gataagacgc
    54901 gataagcgtc gcatcaggca tggtgctcca gacgccgcaa cttacagctg tgacgcctgc
    54961 tgaaacaggt tttcccacat atcggtcacc agcgcctggt cacgcggcga caattcaccg
    55021 gcaccaatgg ctttttccag actctggcta acggtcgtat gcaccgcctg agcggagtgg
    55081 tcatcaccac tttccagttc tgcgatggct aatgtcaggt ggccacgcaa atacccactg
    55141 gcaaacaact catcatcact ggcatggtca accatgccgt cgattaatgc cagaatgcgt
    55201 gattcaaact ccgcgatcat cttctttcct cattgtaaaa cgtggcgctg gcttcagttg
    55261 agcgcttccg gccacgggaa ctgttccgcc gtaagttccg gcgtgtgata ataattttgt
    55321 agtgctttaa tgaagcgtgc cgggcgttcc ggaatgccat ttgtcagata atccatcacc
    55381 tgcgcatgta cccgccgttg aaacaccaca cgatccggtt cgaaatcccc ttccagattg
    55441 tcgcaactga cgttaaacgg atatccagcc gccacgcaga ataaccagtc gaacgcctgc
    55501 ggcttcactt caacatcttc aaactggctt tgcgtctggg catcgcgtcc gtccgggcaa
    55561 taccagtagc cgaaatcaac cagctcacgg cgcgcttttc cggcaataca ccagtgcgaa
    55621 atctcatgaa ttgcgctggc ataaaagcca tgagcaaaga cgattcggtt atacggtact
    55681 tccgcatcag caggaagata gatcggttcg tcgtcgcctt taatcagacg ggtattaaat
    55741 tcatcggcaa agcagctatt aaaaatttca atcaactgct cgtagtggtg tgtactgttc
    55801 attagttcat ccccaaccag tggaggatct cctgtccgtg gctatcgtaa agtaatttgg
    55861 cactcatcac cgccgagacg ataacaatca tcgggcggat cagcttttgt cctttgctca
    55921 acaccaaccg tgaacccatg cgcgcgccga ggaactgccc caccagcatg acaaagcccg
    55981 tcgcccaaat cactttgccg ccgagaataa acagcagcag accgccaatg tttgacgttg
    56041 cgttgagtaa tttggcatga gccgtggctt tggcgaggtt gaatccgcac agcataacga
    56101 aggccagcgc gtaaaacgat ccggcagccg ggccaaagaa tccgtcataa aaaccgacgc
    56161 agccaccggc aattaacgcg aagggtaaac cgtacatccg gcgctggcga tcttcttcac
    56221 ccagctttgg catcagcaag aaatagaggc cgatacaaat caccagaatg ggcaaaatct
    56281 gccgtaagac atcagcctga acgtactgta ccaacagtgc gccgctcatt gagccgacaa
    56341 aggtcatggc aatattgagt ttctgatcgc ttaaactaac cactttgcgg cgaataaagt
    56401 agatagtggc ggaaatagag ccgccgcagg cttgcagttt attggttgcc agcgcgttag
    56461 cgggagacat tcccgcagcc atcaatgccg gaatggtgag taacccaccg ccaccggcaa
    56521 tcgagtcgat aaatcccgcc agcatggcga caaaaaagag aactcccagc aacagcggag
    56581 aaaccataaa caggctatta aacgtttcca ttagatcacg tgctcatcca gtagcgcctg
    56641 gcaggaaggc ggcaacggag gcggtgtctt cttctcaggc tttgttgttc ccggttttgg
    56701 aggttcaaac cagctttgca gttctgcccc gcaaccatcg cctgatggcg gtaaaggttg
    56761 atcttcacac tccagactat cggcagggca acgtaatcgt acatgcatat gcgcgcgatg
    56821 ctggaaccag ggtcgcactt tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag
    56881 ttgttgttta atcgccggat taacaaaaat gcgcgtgacg tctttgtcct gggcggcgag
    56941 tttgatcaag ctgaaaattt ctggcttcca cagcgtggag acaacgtgtt taccgtcgcg
    57001 ggaaactaag tccagtgctt gcgggcgcaa gagctgcgcg gaggtccagc gagttttcgg
    57061 cagttgcaga aagatatcga catccagtcc ggtctggtgg ctggcatgac cgccgttgaa
    57121 acgcccacca gcgggcatcc ccatatcgcc aatcagcacc gtacccatgc ccagattgct
    57181 cacctggcta ctcagacgct ggataaacat caccagatcc gggtgaccga aatagcgacg
    57241 ctgatcggta cgcatgacct gataatgttc ggactgtatc ggcaacgtgt cagcgccgac
    57301 aatacagcca ttagaaaaac tgcctatcga ttgtgcgcta cccggcacag gttgggttat
    57361 tttttgccac ggcgtcgctg ccaggctagc actactggca agcagagcca gcagcgcaat
    57421 cgcggttttg ttcatttttt accagcgtgg aatatcagtc ttcacatcgg cattttgcgc
    57481 ccgctgccgt aacaggtgat ccattaaaac gatcgccagc atcgcttcag cgatcggcac
    57541 tgcacggatc ccgacacagg gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc
    57601 aaagcggtta atagtgcgcc ccggcacggt aatgctggag gtcggtttca gcgccatatg
    57661 agcaatgatt tgctgcccgc tgctgatacc gccgaaaatg ccgcccgcat ggttgctctg
    57721 gaaaccgtct ttggtgattt cgtcgcggtt ctggctgccg cgcagcgcca ctacgtcaaa
    57781 accgtcgcca atttccacgc ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc
    57841 ggcatccagg cggtcaaaga ccggctcgcc aagtccggcg gggacaccac tggcaacaac
    57901 ggtgactttc gcgccgatgg agtcgccctc ttttttcagc gcgcgcatca gttcatctaa
    57961 cgcgtcgatt ttgtcggggt ccgggcagaa gaacggattt tgttcaacca gcgaccagtc
    58021 tttgatttcc agcggaatgt cgcccatctg ggtcaggcag ccgcggattt caataccaaa
    58081 tttctcggcg agatattttt tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg
    58141 ggcggaagaa cgtccaccgc cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta
    58201 atcagcatgg cctggacgga agacgtcttt aattgcgctg taatcctgag aacgctggtc
    58261 ggtattttcg atcaacaagc caatgctggt gccagtagta acgccttcaa aaacaccgga
    58321 gagtattttg acctgatccg gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg
    58381 acgacgatca aggtcatgtt gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc
    58441 atcgacgatg cagccgagcg ccagcccgtg cgattcgccg aaggtggtta cgcgaaagag
    58501 ttgtccaatt gtgtttccag ccatcacggc tccgttattg ttgtgtttgc gtgtttactt
    58561 aatctttata aatcgcgaaa tgttctcgtg cggcaataag ctgctcttta gtgagcataa
    58621 acacaccatc gccgccgtta tcaaactcca gccaggtgaa cggaacatcg ggatattgtt
    58681 ccataagatg taccatgctg ttgcccactt cacaaatcaa cacgccatca tcagcaaggt
    58741 aatctgccgc gttaccgaga atgcgacgcg tcagtttcag gccgtcggtg ccagatgcca
    58801 ggcccagttc cggctcgtga cggtattcgt ttggcaggtc ggacatatct tccgcgtcga
    58861 catacggcgg gttagtgaca atcagatcgt attgcacttt cggcaaatca cggaacagat
    58921 cggaacgaat cggaatgacg ttgtggatca gaccgtgttc ttcgatgttc tgttcggcaa
    58981 ccgccagcgc gtcgggagag atatctaccg cgtcgacttc cgcttccggg aaggcataag
    59041 cacaggcaat ggcgatgcag ccgctaccgg tacacatatc taaaatatgc tgcggttgct
    59101 tgctgatgag tccggcaaat ttattgttga tcagttcacc aatcggcgag cgcggcacca
    59161 gcacgcgttc atcgacgtaa aattcatgac cgcagaacca ggctttgttg gtcaggtaag
    59221 ccaccggaat gcgctcgttg acgcggcgga tcacgcgttc aacaatacgg tgtttttcgc
    59281 tggaggtcag acgcgcggtg cgcatatctt ccgggatatc cagcggcagg tagagcgatg
    59341 gtaacaccag ctgtacggct tcatcccacg ggttatcggt gccgtgaccg taccagatat
    59401 ttgccgcgct gaagcggctt accgaccagc gcaatatgtc ctgaatggtt tgcagctcat
    59461 ttactgcttc atcaacgaaa attttatcca cgtattcctc cagggcatga tcgcaataat
    59521 ttcggcggct agtttgccat gaagatgacg ataaatcagc attcacgcgc ggtgagtgag
    59581 gaaaaatacg tttaaaacga tcgattgtgc tacgagtcgg gtaaactgta ggaaaattag
    59641 aaatagagac agataaatga aaaagaaaac aacacttagc gaggaggacc aggctctgtt
    59701 tcgccagttg atggcgggga ctcgcaagat taagcaggac acgattgtcc accgaccgca
    59761 gcgtaaaaaa atcagcgaag tgccggtgaa acgcttgatc caggagcagg ctgatgccag
    59821 ccattatttc tccgatgagt ttcagccgtt attaaatacc gaaggtccgg tgaaatatgt
    59881 tcgcccggat gtcagccatt ttgaggcgaa gaaactgcgc cgcggcgatt attcaccgga
    59941 attgtttttg gatttacacg gtctgacgca gctgcaggcc aagcaggaac tgggcgcgtt
    60001 gattgccgcc tgtcgccgcg aacatgtgtt ttgtgcctgc gtgatgcatg ggcacgggaa
    60061 gcatattttg aagcaacaaa caccgctgtg gctggcacaa catccgcatg tgatggcatt
    60121 tcaccaggca ccgaaagagt acggcggtga tgcggctttg ttggtgttga ttgaagtgga
    60181 agagtggttg ccgccggagt tgccctgaat gctggcagca aatgccggag cagcgtaaac
    60241 gccttatccg gcctacaaat atgtgataat tcaataaatt gcaattcact tcgtaggcct
    60301 gataagacgc atcagagcaa tactacgaac ttcagttcat tcaatacata ctgaaaacga
    60361 cacgtcgaat caatcggttg cccactactg gtattcacat tcaccacaat atcgtgtagt
    60421 actttatccg ttaccggatg acgagttggc gtaaccgtta ccgttgattt atcacaaccg
    60481 taagaggtaa aggttaacaa ttctgatacc gcgcggcagt atgctcctct gccatcatta
    60541 tcagaaaaat tactgccatc acagagtctg ccgccttcca tcaggtaggt cgaaagcgtg
    60601 gtggtcactg ttgaccccgt ggactgaatt cgtaacgcct tattatggct gacataattt
    60661 tcactccagg agatatcgta tgaagcgacc ggtatcgttg ggcatattgt gttatgcact
    60721 gtaggggccg tatagcttcc aaatccgggt aaatctgtat aggaataaaa ggatgcgatc
    60781 caagggcaac cataatcatc agatgcaacg gtaattttaa acgtcatata tgcacaacga
    60841 aaaattttcg tatctatgcc agggcaattt gagttattaa ttgtatcaaa gtcagtcagt
    60901 accaaagttg gagctgaacc cgacccagac caggaaataa cccgcaacgt atcgccttta
    60961 tacgcaaaag aatcatattt cccatatgca acctcctttg gcgttatcac cgtaacattg
    61021 aaaacactgc tcgctacagc accggttacc ttgtccatgg cataataaac tctcacactg
    61081 tcaaaactcg cactcataag tttatctcca gcataactct tagatgcgcc gacaacactc
    61141 agaacgagca gcattaggct cgcgaaaatt atggtccatt ttttcatttc agaaatactc
    61201 aaaggttgcc gtcactgtgg cactaaagtc tccggacgtt acatctcgcc cgctctttgc
    61261 ctgtaaccag gtattaaaat ttaggctgtt atttccctga ttcagcggga gcgtgacgcc
    61321 tgttgtattg ttgattggca ccgatgtgcc tgccgcagtt tcaataccaa tcccagcgcc
    61381 ggatgctgat gacgaggagt caaaggccag aaagccgggt agagcactat cttctgttcc
    61441 tgtcaacgtt actttgacat taaacaatgt agaactatga caatctttca attgaaacac
    61501 caatggcact cgctctgact gcccaaggtt cattaaatca ctggcggcaa tggcaggaaa
    61561 atgtacttct gccaactggc taccattaat cactggcgta cacgatttac tgattaaatt
    61621 gccggtaaag tgcaaattat ctcctgctat tgtggttcca ctgcccagca acatcaggct
    61681 actaataaaa aacaacgtac gctttttctt cattggtaat ctacctgtaa cgtggcccag
    61741 gcctcaaagt ccgcctctgg cagaatgaca ccactctttt ttactggcac agccgttaac
    61801 accggtaaat cggtttcatt caccacaagt ggcgtattga tcgtaaaact ctgccctgcc
    61861 tgtttgagct gaatgcctaa accagtgata ttgctgctta ctgcagcatt atcaaaatcg
    61921 cttggtgtgc cactccagct caaggtcatt tccattaatg ggtcgcgaac cgccgtatcg
    61981 caggtaatgg aatacggcac ggtttgctca taattgctgc cattaatgtc atcaataagc
    62041 acatcctgaa aagttatttc aatggtttga gcgttgctaa tcgtgcagtt aggcggctgc
    62101 actaaataac cgtgaaaaga gacgtttgag agtgccatcg agcaaaagcc ccacagcaaa
    62161 ataagcgaga tccgtttcat tggtaatcca ccacaattgt ggcactggcg ttaaattcag
    62221 ctgccgctaa cgttgtacca ctctgtttca ccggcacagc ttccagaacg ggaactcccg
    62281 aggagaaatt aaatggcaac catgagccag aggttaagtc gagtattgtg tgatcactac
    62341 ttttctgaac ccgaatacca aacccaggaa tacccgtgct gataactgtt tcaccgttaa
    62401 tcacgaccgt tgtcgcctgc atctgtagac gcaggtcatc cattgccaga ttgttacaaa
    62461 caagcgagta gtcaatggga cgtttataat caaccctatt gatcttatta atgaatacat
    62521 taccaaactc aactgccgca ccatttaccg tacaaggagg aggggcatcg acaagcaccc
    62581 gtaaattcat acttactgac ttactcgctg caactgcaaa gccgctacca acacaacata
    62641 tcagtaaccc agttaccgcc cagacagaac aagatccttt catactggct ctcccggcct
    62701 cagcctttgc ggctctgctc ttcatcgacc tagcaactgt taccgttgca agtgaaaaag
    62761 agcggcatcc gagcgccata gtcgttgaca taggtcagta ccggcacgct acccatcttc
    62821 acattcagcg gtgcagaggt tttcggcgac atcactattg gcgagaaacc ggctgccgga
    62881 ttgccatttt tctgtgtgct ggcattactg aaaatgacgt aatacgacgt cgggttattg
    62941 acggtaaatt cctgaccgtt gcgggttaaa gtaatcttat attgctaggg gtttttcatg
    63001 ctgacgttct ccagcgcctt cggtcgccag aacaacttaa tacgcgtttg tagtgctatt
    63061 tgcagcgtgt tggctttgtt tgattttggc gggatctcac gcacgttgaa gtagaacagg
    63121 ctttcacgat cggcaggcaa tttgttgata tccggcattc cctgcacttt tacctgcccg
    63181 ttcatcattg aatcgatgcg ttgcaccggt ggcagaacag ttaacggtga actgatcttg
    63241 ttaccctttt catcttcgat ccagctttgg gccagataag gcagtttcgg atcgttattg
    63301 cgcaacgtca cgctgattga tttgtcgctt tcattaaata tcaaacgggt acgatctggc
    63361 gttacagaag cctgggcaga caaactggca gcgctaagca gtaacgctag cgtcgtagca
    63421 cccaatttgg cagagcaaag taaatcagac atggatcatt ccttaatcac gttattggtt
    63481 agctgaaaca gaaacagggg gattggtggg tgtcacctgt tgcgtctgct cctgaatgac
    63541 aggctttatg tcatcaggaa caaccggcgc gacgttgcct ttatgctggc atggcagtaa
    63601 caagccgtta aacagatcgg caggcagcgg gtccggcagg ttgatatcgc aatgcgcaac
    63661 accactccag aacacactca tgtgttcgcc aggtttcact ccagccagat aaacattgcc
    63721 gtcatcatcg acaaggccca ctgtctgctc gttatcattt tttacttctg cgccaaacgg
    63781 aggatggctg ccgtcttgta aacgcagcac cgccatcgct ttttgaccac tgatgacggc
    63841 aaatttgcgg tagccaatcg ccccttcagt tagcgtggct tgtaccaccg actgggttgc
    63901 ttcggcgttt tccggcagtt tgttgaggtc gatatacgcc tgattgcggt aatagttatt
    63961 gacgtcagaa acgacggctt taccaaacat attggtataa acagcagcac cgttaccttc
    64021 taccggaaca tcggcaacgc catcggcatc aatcaacaag cgtgtcccgc ccatattctg
    64081 ggtacggtga agtgcgccac cgtgggcagt aagcgttgcg ccgccctgta acgacaagcc
    64141 cgcagaggtg tattgccctt catggtagtt cgcactgagg tcaacctgcg ccagcgaacc
    64201 atcatggctg taatagccgt caacgctggt gtgtttgtca ctggtgccga cgttcaactg
    64261 atagtgagtc gcgtcatcga cacggctgaa ataaccgacc tgactgctgt ccgtcccact
    64321 gccatagtac cgttatagct aacggtactg ttgtcgcccc acggcatact gagcgaaatg
    64381 tacatgcctt tgtcggcctg gttgtcatac tcgtaacggt agccagtcat cgagatgctg
    64441 acattacgaa tactgcccat attgaaatag tgcgagagca tgatgttgta gtttgtctgc
    64501 tcctcgcgat cccagtaggt atggcgggta tagttgagat aaaccgaaac acccgcatcg
    64561 cggaagttct ggttataagt ggcggtgtac atctctttgt cgttgcccgt gcgtaccatt
    64621 ccgctgtcac tggcatccag atactcgctc atggtcataa agttctcttc cgagaagcga
    64681 tatccagcaa aagtaacgcg gctgttgagc tggtcaaagt ctttggaata actcacacgg
    64741 aaggagttac cgtccagcga acctttgcca taagcggtgt ctttatccag tttggtgtgc
    64801 gagtgagtaa catcaaacgc gaccgcgccg aatgtagaca aatcgcgacc gacaccaagc
    64861 gccgcagact gatagttttc atctcccagt gcgccgccat ataacgacca gccgttagcg
    64921 atcccccagg aagcttcagc accagaaaaa aattcaccct cgacatggtg tccccactct
    64981 tgcggacggc ccatcatgat cttatagcga acctgacctg gacgagtgag gtatggcatc
    65041 gaggcggtgc tgatgtcata ttcctgcacc tggccgttct gttcttcaat gcgaatatgc
    65101 aacgtaccgg agacagaatc accaagatcc tgaatacgaa acggtccagc cggaacctgc
    65161 gtttcgtaaa tcacacgccc catctggctg acggtcactt ttgctgtggt gtgtgccacg
    65221 ccggaaatgt ctggcgcgta gccgcgcaga ttgggaggca acatttgatc gtcagtactg
    65281 acactgccac caacatagtt aaaaccatca aaaatatcgg atctgaggta atcctcgccc
    65341 agcgccagtt tggcttttaa tgatggtaac gcccgcctgg catagtagcg actccactcc
    65401 catttttttg agtttcatcg ccgctgaatt catcgtcatc attactgcga gtatgttgat
    65461 agttagtctg ccagtcagca cgcatacgcc acggccctag gttaacccca accgtcccgt
    65521 tgccgctgat ctcgttacta tcatcaccgc cattttcttc gtgccgggtt tgtgcgttga
    65581 tgctgtagtc cgcgacgatc ccggagatgc cgtcatccca acgtgaaggc ggatccctgt
    65641 cgggataagt atattcgagg taagcctgtg gcagtgaaat gactaatgcg gactggctta
    65701 aatcagcctt aatttccatg ccttccagtt gaccggattt caggcattta gcatcgtggc
    65761 tccattgcag atttttcgcc acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg
    65821 tcagacaagc atagcttttg ctcgcgtcat cttcaccagc ataccagtaa atatcgtact
    65881 cttccgccaa tggctgttta tttagttgaa cctgtaaatt gtattttccg ggctcaacat
    65941 atccctggct ggaaaaacgc ttcatatcaa tttttgtgtc gccttttaat tccagaaaac
    66001 gggaatcaaa ctgaatgtcg tcttcagccc agacactgct ataactccct gacattgcca
    66061 gcgcaatgca ccacggaaga atccgtaatc gaaaaagaga atggtcaggc atacacgttt
    66121 ctatccgttt ttattcttgt tcggtcaaaa tacatttaaa tcccccatag cagtgtcgct
    66181 atgggggtat gccttacagg taagtaatct ggaaggtggt atttgcttca aaattaccca
    66241 gatctggcgc atcagctgcg ccgaccagcc aggcgttaaa gttcagtgtc tgcttcgctt
    66301 tgcctttgtt ggtagaagtg tctttcacta ttttctggtc aatacccatg ccgcttttgt
    66361 aagaggtacc cagagagtca ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat
    66421 cggtattgaa aatggtgtaa taattgccgc tatttgcaga agaaacggta ccagtaaagg
    66481 tagtggtcat tgtttcctga gtatcaaata cgcaatcttg cagacgaatc tggaaacttt
    66541 tcggcgtggt ggtgccgtta tttttcagtt tttcggcacc gatatcaccc atatcaactt
    66601 ccagtttgtg atcgtccgga acgatagaac acactgagtc ttcaatggtg ccgtaaaaac
    66661 gtgcagtacc gttgttaccc gcagcgattg ttgccgtaga ggtgaacacg cccatcacca
    66721 ttgccgcagc aatagctgtt ttaacaaact tactcattat ttgtccttaa actataaata
    66781 attaaaaata taaacattgc aatacattga ttcagtcaat agccaatgtt tatacccagc
    66841 cattgaatac ggcatgcgac ccaaaaagaa caggtcgtag aaaattgtat tcattggtgt
    66901 ggggccgcat agtgatgttt ccacacccct gatccaattt aattttacta aagagtaaat
    66961 gtagatttaa ttaatatatt gacagaagaa aggttatctt aagga
//
LOCUS       DAZOZX010000004        60419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZOZX010000004 DAZOZX010000000
VERSION     DAZOZX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.004"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..322
                     /locus_tag="ACHUPT_000222"
     CDS             <1..322
                     /locus_tag="ACHUPT_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0817971.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHUPT_000223"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHUPT_000223"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0817972.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHUPT_000224"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHUPT_000224"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0817973.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUPT_000225"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUPT_000225"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0817974.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHUPT_000226"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHUPT_000226"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0817975.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHUPT_000227"
     CDS             complement(3726..4955)
                     /locus_tag="ACHUPT_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0817976.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHUPT_000228"
     CDS             complement(5019..5459)
                     /locus_tag="ACHUPT_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0817977.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHUPT_000229"
     CDS             complement(5564..6334)
                     /locus_tag="ACHUPT_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0817978.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHUPT_000230"
     CDS             complement(6331..7257)
                     /locus_tag="ACHUPT_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0817979.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHUPT_000231"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHUPT_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0817980.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUPT_000232"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUPT_000232"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0817981.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUPT_000233"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUPT_000233"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0817982.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUPT_000234"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUPT_000234"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0817983.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHUPT_000235"
     CDS             12964..13311
                     /locus_tag="ACHUPT_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0817984.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHUPT_000236"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHUPT_000236"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0817985.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHUPT_000237"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHUPT_000237"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0817986.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUPT_000238"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUPT_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0817987.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUPT_000239"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUPT_000239"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0817988.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACHUPT_000240"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACHUPT_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUPT_000241"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUPT_000241"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0817989.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUPT_000242"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUPT_000242"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0817990.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUPT_000243"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUPT_000243"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0817991.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUPT_000244"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUPT_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0817992.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUPT_000245"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUPT_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0817993.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUPT_000246"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUPT_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0817994.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUPT_000247"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUPT_000247"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0817995.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUPT_000248"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUPT_000248"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0817996.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUPT_000249"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUPT_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0817997.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACHUPT_000250"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACHUPT_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUPT_000251"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUPT_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR0817998.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUPT_000252"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUPT_000252"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0817999.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACHUPT_000253"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACHUPT_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUPT_000254"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUPT_000254"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0818000.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUPT_000255"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUPT_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0818001.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUPT_000256"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUPT_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0818002.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUPT_000257"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUPT_000257"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0818003.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUPT_000258"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUPT_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0818004.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUPT_000259"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUPT_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0818005.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNPNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUPT_000260"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUPT_000260"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0818006.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUPT_000261"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUPT_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0818007.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUPT_000262"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUPT_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0818008.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUPT_000263"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUPT_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0818009.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUPT_000264"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUPT_000264"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0818010.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUPT_000265"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUPT_000265"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0818011.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUPT_000266"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUPT_000266"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0818012.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUPT_000267"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUPT_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0818013.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUPT_000268"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUPT_000268"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0818014.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUPT_000269"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUPT_000269"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0818015.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUPT_000270"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUPT_000270"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0818016.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUPT_000271"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUPT_000271"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0818017.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUPT_000272"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUPT_000272"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0818018.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUPT_000273"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUPT_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0818019.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUPT_000274"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUPT_000274"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0818020.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUPT_000275"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUPT_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0818021.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16732 c        14848 g        14366 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctaccca aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 caccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcgg attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAZOZX010000005        59768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZOZX010000005 DAZOZX010000000
VERSION     DAZOZX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.005"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            67..1755
                     /locus_tag="ACHUPT_000276"
     CDS             67..1755
                     /locus_tag="ACHUPT_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR0818022.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            1752..2666
                     /locus_tag="ACHUPT_000277"
     CDS             1752..2666
                     /locus_tag="ACHUPT_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0818023.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2699..2947
                     /locus_tag="ACHUPT_000278"
     CDS             2699..2947
                     /locus_tag="ACHUPT_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0818024.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2947..4118
                     /locus_tag="ACHUPT_000279"
                     /pseudo
     CDS             2947..4118
                     /locus_tag="ACHUPT_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(4154..5233)
                     /gene="lptG"
                     /locus_tag="ACHUPT_000280"
     CDS             complement(4154..5233)
                     /gene="lptG"
                     /locus_tag="ACHUPT_000280"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0818025.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5230..6300)
                     /gene="lptF"
                     /locus_tag="ACHUPT_000281"
     CDS             complement(5230..6300)
                     /gene="lptF"
                     /locus_tag="ACHUPT_000281"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0818026.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6331..6891)
                     /locus_tag="ACHUPT_000282"
     CDS             complement(6331..6891)
                     /locus_tag="ACHUPT_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0818027.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6903..7727)
                     /locus_tag="ACHUPT_000283"
     CDS             complement(6903..7727)
                     /locus_tag="ACHUPT_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818028.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(7727..9076)
                     /locus_tag="ACHUPT_000284"
     CDS             complement(7727..9076)
                     /locus_tag="ACHUPT_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0818029.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9122..9880)
                     /locus_tag="ACHUPT_000285"
     CDS             complement(9122..9880)
                     /locus_tag="ACHUPT_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818030.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(9912..10625)
                     /locus_tag="ACHUPT_000286"
     CDS             complement(9912..10625)
                     /locus_tag="ACHUPT_000286"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0818031.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            10799..11491
                     /locus_tag="ACHUPT_000287"
     CDS             10799..11491
                     /locus_tag="ACHUPT_000287"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0818032.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11540..13039)
                     /gene="pitB"
                     /locus_tag="ACHUPT_000288"
     CDS             complement(11540..13039)
                     /gene="pitB"
                     /locus_tag="ACHUPT_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0818033.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13331..15190)
                     /gene="gss"
                     /locus_tag="ACHUPT_000289"
     CDS             complement(13331..15190)
                     /gene="gss"
                     /locus_tag="ACHUPT_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0818034.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15395..16261
                     /gene="yghU"
                     /locus_tag="ACHUPT_000290"
     CDS             15395..16261
                     /gene="yghU"
                     /locus_tag="ACHUPT_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0818035.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16290..16371)
                     /locus_tag="ACHUPT_000291"
                     /pseudo
     CDS             complement(16290..16371)
                     /locus_tag="ACHUPT_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16384..16632)
                     /gene="hybG"
                     /locus_tag="ACHUPT_000292"
     CDS             complement(16384..16632)
                     /gene="hybG"
                     /locus_tag="ACHUPT_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0818036.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16766..17107)
                     /gene="hypA"
                     /locus_tag="ACHUPT_000293"
     CDS             complement(16766..17107)
                     /gene="hypA"
                     /locus_tag="ACHUPT_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0818037.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17100..17588)
                     /gene="hybE"
                     /locus_tag="ACHUPT_000294"
     CDS             complement(17100..17588)
                     /gene="hybE"
                     /locus_tag="ACHUPT_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0818038.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(17581..18075)
                     /locus_tag="ACHUPT_000295"
     CDS             complement(17581..18075)
                     /locus_tag="ACHUPT_000295"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0818039.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18075..19778)
                     /gene="hybC"
                     /locus_tag="ACHUPT_000296"
     CDS             complement(18075..19778)
                     /gene="hybC"
                     /locus_tag="ACHUPT_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0818040.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19775..20953)
                     /gene="hybB"
                     /locus_tag="ACHUPT_000297"
     CDS             complement(19775..20953)
                     /gene="hybB"
                     /locus_tag="ACHUPT_000297"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0818041.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20943..21929)
                     /gene="hybA"
                     /locus_tag="ACHUPT_000298"
     CDS             complement(20943..21929)
                     /gene="hybA"
                     /locus_tag="ACHUPT_000298"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0818042.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21932..23050)
                     /gene="hybO"
                     /locus_tag="ACHUPT_000299"
     CDS             complement(21932..23050)
                     /gene="hybO"
                     /locus_tag="ACHUPT_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0818043.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23239..23526)
                     /gene="yghW"
                     /locus_tag="ACHUPT_000300"
     CDS             complement(23239..23526)
                     /gene="yghW"
                     /locus_tag="ACHUPT_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0818044.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23645..24532)
                     /gene="yghX"
                     /locus_tag="ACHUPT_000301"
     CDS             complement(23645..24532)
                     /gene="yghX"
                     /locus_tag="ACHUPT_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0818045.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24689..25729
                     /gene="gpr"
                     /locus_tag="ACHUPT_000302"
     CDS             24689..25729
                     /gene="gpr"
                     /locus_tag="ACHUPT_000302"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0818046.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25769..26263)
                     /locus_tag="ACHUPT_000303"
     CDS             complement(25769..26263)
                     /locus_tag="ACHUPT_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0818047.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26454..27338
                     /gene="yghA"
                     /locus_tag="ACHUPT_000304"
     CDS             26454..27338
                     /gene="yghA"
                     /locus_tag="ACHUPT_000304"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0818048.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27610..28035)
                     /gene="exbD"
                     /locus_tag="ACHUPT_000305"
     CDS             complement(27610..28035)
                     /gene="exbD"
                     /locus_tag="ACHUPT_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0818049.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28042..28776)
                     /gene="exbB"
                     /locus_tag="ACHUPT_000306"
     CDS             complement(28042..28776)
                     /gene="exbB"
                     /locus_tag="ACHUPT_000306"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0818050.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29028..30215
                     /gene="metC"
                     /locus_tag="ACHUPT_000307"
     CDS             29028..30215
                     /gene="metC"
                     /locus_tag="ACHUPT_000307"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0818051.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30355..31014
                     /gene="yghB"
                     /locus_tag="ACHUPT_000308"
     CDS             30355..31014
                     /gene="yghB"
                     /locus_tag="ACHUPT_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0818052.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31054..31953)
                     /gene="yqhC"
                     /locus_tag="ACHUPT_000309"
     CDS             complement(31054..31953)
                     /gene="yqhC"
                     /locus_tag="ACHUPT_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0818053.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32147..33310
                     /gene="yqhD"
                     /locus_tag="ACHUPT_000310"
     CDS             32147..33310
                     /gene="yqhD"
                     /locus_tag="ACHUPT_000310"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0818054.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33415..34242
                     /gene="dkgA"
                     /locus_tag="ACHUPT_000311"
     CDS             33415..34242
                     /gene="dkgA"
                     /locus_tag="ACHUPT_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0818055.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            34442..35368
                     /locus_tag="ACHUPT_000312"
     CDS             34442..35368
                     /locus_tag="ACHUPT_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0818056.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35419..35676
                     /gene="yqhH"
                     /locus_tag="ACHUPT_000313"
     CDS             35419..35676
                     /gene="yqhH"
                     /locus_tag="ACHUPT_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0818057.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35719..37938)
                     /locus_tag="ACHUPT_000314"
     CDS             complement(35719..37938)
                     /locus_tag="ACHUPT_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0818058.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(38049..39461)
                     /gene="ftsP"
                     /locus_tag="ACHUPT_000315"
     CDS             complement(38049..39461)
                     /gene="ftsP"
                     /locus_tag="ACHUPT_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0818059.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39536..40273)
                     /gene="plsC"
                     /locus_tag="ACHUPT_000316"
                     /gene_synonym="parF"
     CDS             complement(39536..40273)
                     /gene="plsC"
                     /locus_tag="ACHUPT_000316"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0818060.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40507..42765)
                     /gene="parC"
                     /locus_tag="ACHUPT_000317"
     CDS             complement(40507..42765)
                     /gene="parC"
                     /locus_tag="ACHUPT_000317"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0818061.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43311..43793)
                     /locus_tag="ACHUPT_000318"
     CDS             complement(43311..43793)
                     /locus_tag="ACHUPT_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0818062.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(43846..44238)
                     /gene="ygiW"
                     /locus_tag="ACHUPT_000319"
     CDS             complement(43846..44238)
                     /gene="ygiW"
                     /locus_tag="ACHUPT_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0818063.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            44390..45049
                     /gene="qseB"
                     /locus_tag="ACHUPT_000320"
     CDS             44390..45049
                     /gene="qseB"
                     /locus_tag="ACHUPT_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0818064.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            45046..46320
                     /gene="qseC"
                     /locus_tag="ACHUPT_000321"
     CDS             45046..46320
                     /gene="qseC"
                     /locus_tag="ACHUPT_000321"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0818065.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(46366..46698)
                     /locus_tag="ACHUPT_000322"
     CDS             complement(46366..46698)
                     /locus_tag="ACHUPT_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR0818066.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            47017..47598
                     /gene="mdaB"
                     /locus_tag="ACHUPT_000323"
     CDS             47017..47598
                     /gene="mdaB"
                     /locus_tag="ACHUPT_000323"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0818067.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            47629..47943
                     /locus_tag="ACHUPT_000324"
     CDS             47629..47943
                     /locus_tag="ACHUPT_000324"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0818068.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(47991..49883)
                     /gene="parE"
                     /locus_tag="ACHUPT_000325"
     CDS             complement(47991..49883)
                     /gene="parE"
                     /locus_tag="ACHUPT_000325"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0818069.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(49912..50493)
                     /gene="yqiA"
                     /locus_tag="ACHUPT_000326"
     CDS             complement(49912..50493)
                     /gene="yqiA"
                     /locus_tag="ACHUPT_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0818070.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(50493..51320)
                     /gene="cpdA"
                     /locus_tag="ACHUPT_000327"
     CDS             complement(50493..51320)
                     /gene="cpdA"
                     /locus_tag="ACHUPT_000327"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0818071.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(51345..51767)
                     /locus_tag="ACHUPT_000328"
     CDS             complement(51345..51767)
                     /locus_tag="ACHUPT_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0818072.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(51768..52397)
                     /gene="nudF"
                     /locus_tag="ACHUPT_000329"
     CDS             complement(51768..52397)
                     /gene="nudF"
                     /locus_tag="ACHUPT_000329"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0818073.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            52602..54083
                     /gene="tolC"
                     /locus_tag="ACHUPT_000330"
     CDS             52602..54083
                     /gene="tolC"
                     /locus_tag="ACHUPT_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0818074.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            54231..54902
                     /locus_tag="ACHUPT_000331"
     CDS             54231..54902
                     /locus_tag="ACHUPT_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0818075.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            54908..56068
                     /locus_tag="ACHUPT_000332"
     CDS             54908..56068
                     /locus_tag="ACHUPT_000332"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0818076.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(56106..56894)
                     /gene="ygiD"
                     /locus_tag="ACHUPT_000333"
     CDS             complement(56106..56894)
                     /gene="ygiD"
                     /locus_tag="ACHUPT_000333"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0818077.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            57037..57810
                     /gene="zupT"
                     /locus_tag="ACHUPT_000334"
     CDS             57037..57810
                     /gene="zupT"
                     /locus_tag="ACHUPT_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0818078.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(57868..58038)
                     /gene="yqiD"
                     /locus_tag="ACHUPT_000335"
     CDS             complement(57868..58038)
                     /gene="yqiD"
                     /locus_tag="ACHUPT_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0818079.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(58299..58952)
                     /gene="ribB"
                     /locus_tag="ACHUPT_000336"
     CDS             complement(58299..58952)
                     /gene="ribB"
                     /locus_tag="ACHUPT_000336"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0818080.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(59056..59204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            59326..59616
                     /gene="ubiK"
                     /locus_tag="ACHUPT_000337"
     CDS             59326..59616
                     /gene="ubiK"
                     /locus_tag="ACHUPT_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0818081.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        14958 a        15853 c        14828 g        14129 t
ORIGIN      
        1 ttatttttga tatccaattt tttcgtggtg tatatgtcta ataaaatctt tacgcattcc
       61 ctacctatgc gctatgccga ttttccaacg ctggttgatg ctttggacta cgccgctctg
      121 agtagagccg gaatgaattt ttatgacaga cgttgccaac ttgaagatca actggaatat
      181 cagacattaa aaacgcgtgc cgaagctggt gcgaagcggt tgttatcgct gaacctgaaa
      241 aaaggcgaac gcgtggcgct gattgccgaa acgagtagcg ggtttgtaga ggcttttttt
      301 gcctgtcagt atgctggctt agtcgccgtc ccgttggcga ttccaatggg cgtcggtcag
      361 cgggattcct ggagcgccaa actccagggt ttactggcaa gttgccagcc cgcagccatt
      421 atcactggtg atgagtggtt gccactggtc aatgccgcga cgcataacaa ccccgaatta
      481 catgttttaa gccacgcttg gtttaaggca ttaccggaag ccgatgttgc gctccagcgt
      541 ccagttccga acgatatcgc ctacctccag tacacctccg gcagcacccg ttttccccgt
      601 ggcgtcatta tcacccatcg cgaagtgatg gctaatctac gtgctataag ccacgatggc
      661 attaaattac gccctagcga ccgctgcgtc tcctggcagc ctttctacca tgatatggga
      721 ctggtcggct ttctcctgcc ccccgtcgcc acgcaacttt cagtagatta tttgcgcact
      781 caggattttg ccatgcgtcc tttgcaatgg cttaaattga tcagtaaaaa tcgcggcacc
      841 gtttccgttg cgccgccgtt tggctatgaa ttgtgccaac gccgcgtgaa tgaaaaagat
      901 ctcgctgaac tggatctttc ctgctggcgc gtcgctggta ttggtgctga gccgatctcc
      961 gcagaacaac tccatcaatt cgctgaatgt ttccgtcagg ttaactttga caataaaact
     1021 ttcatgccgt gctacggact ggcagaaaat gcgctggctg tcagcttctc tgatgaagcc
     1081 tccggggttg tggttaacga agtggatcgc gacatcctcg aatatcaggg taaagccgtc
     1141 gcgccgggtg cagagacacg cgccgtatcg actttcgtca actgcggcaa agcgttgccg
     1201 gaacatggta ttgaaatccg caatgaagca ggtatgccgg tcgcggaacg tgtggtaggc
     1261 catatttgca tctccggtcc cagtctgatg agcggttact ttggcgacca ggtttcgcaa
     1321 gacgagattg ccgcgacggg ctggttagac accggcgacc tcggttatct gctggacggt
     1381 tatctgtatg tcaccggacg cattaaagat ctgattatta ttcgtggccg taatatctgg
     1441 ccgcaggata ttgaatatat agcggaacag gaaccggaaa ttcattctgg cgatgcgatt
     1501 gcttttgtta ccgcccagga aaaaatcatt ttgcagatcc agtgtcggat cagcgacgaa
     1561 gaacgtcgcg ggcagcttat ccacgcgctg gcggcacgga tccaaagcga atttggcgtg
     1621 accgcggcta tcgatctgtt gccgccccac agtattcccc gaacgtcctc cggcaagcct
     1681 gcccgtgcgg aagcgaaaaa acgttatcag aaggcttatg ctgccagtct taatgtgcag
     1741 gaatccctgg cataaatcaa actgtcgcgg tgacgggcgc taccgggttt atcggtaaat
     1801 atattatcga taacctgctc gcccgcggct ttcacgttcg cgcattgacg cgtactgccc
     1861 gcgctcacgt caatgataat ctcacctggg tgcgcggttc gctggaagat actcattcac
     1921 ttagcgagct ggttgccgga gccagcgtag tggtccattg cgccgggcaa gtgcgcgggc
     1981 acaaagaaga gattttcacc cgctgtaacg ttgacggcag cctgcgcctg atgcaagcag
     2041 caaaagagag cggcttttgc caacgttttc tgtttatctc ttcgctggcg gcgcgccatc
     2101 ccgagctctc ctggtatgca aaatccaaat acgtcgccga gcaacgactg gcagcaatgg
     2161 cggacgaaat tacgctgggc gtttttcgcc cgacagccgt gtatggtccc ggcgataaag
     2221 agttaaaacc gctgtttgac tggatgctac gcggcctgct gccacgactt ggtgcgccag
     2281 atacacaact ctcttttctt cacgtcaccg attttgcgca agcagtgggt cagtggttaa
     2341 gcgccgaaac tgtacagacg caaacctatg aattatgcga tggcgtcgcc ggtggctatg
     2401 actggcaacg cgtacgacag cttgccgcca aagcccgttg tggttccgtg cgaatggttg
     2461 acattcctct gccggtactc acaggccttg cggatatcag taccgcgttg agtcgcctgg
     2521 caggtaaaga acctatgctg acccgctcga aaattcgtga attaacccac gccgactggt
     2581 cggcaagtaa taaccgtatt tctgaagata tcaattggtt tcccgggatt agcctggaac
     2641 acgcattacg caacgggcta ttttgattgt cttgttttaa aggtgtgtta acgtttctat
     2701 ggtaaatcgt gaaatagtaa tggattacat cctttcctgt ttacaggatt tggtcgaaaa
     2761 cggtgtggaa attaagcccg acagcgatct cgtcaacgat cttggcctgg agtcgatcaa
     2821 agtgatggat ctcttaatga tgctggaaga cagattcgat atttccattc ctattaatat
     2881 tctgctggat gtcaaaaccc ctgcgcaatt aatggaaact ctacttccat ggctggagaa
     2941 taaataatgg ggctatacga taaatatgcg cgccttgctg gcgagcgtct gcaatttagt
     3001 gacaacgggt taacaccgtt tggcacctgt attgatgaag tgtattccgc caccgaaggc
     3061 cggatcggca ataaaaaagt gattctggcc gggaccaata attatctcgg tttaaccttt
     3121 aatcatgatg ccatcagcga aggtcaggcc gcgttagcgg cccaaggcac cggcaccacc
     3181 ggttctcgta tggcaaacgg cagttatgcg ccgcacctgg cgttagagaa agagattgcc
     3241 gagtttttca atcgccctac cgctatcgtt tttcaaccgg ttataccgct aatttaggcg
     3301 tcatcagcgc acttgccgac cataacgccg tggtgttact ggatgccgac agccacgcca
     3361 gtatttatga cgtctgttct ctcggtggcg cggaaattat tcgtttccgc cataacgatg
     3421 ctaaagatct ggaacggcgc atggttcgcc tcggtgaacg ggcgaaagag gcgattatca
     3481 tcgttgaggg catctacagc atgctcggtg acgttgcgcc gctggcggaa attgttgata
     3541 tcaaacgtcg tctcggtggt tatttgattg tcgatgaagc gcattcgttt ggcgtgttag
     3601 gcgcaacggg gcgcggactg gctgaagctg tcggcgttga agatgatgtg gatattatcg
     3661 ttggtacttt cagtaaaagt ctggcctcga ttggcggttt tgctgtcgtt tcggaagcga
     3721 tggaagtgct gcgctacggc agccgcccgt atatttttac cgcctctcct tctccgtcct
     3781 gcattgcgac cgtgcgctcg tcgttaagaa ctattgccac acaaccagag ctgcggcaaa
     3841 agctgatgga taacgccaac catctgtatg acggtctgca aaaactgggt tatgagttaa
     3901 gttcacatat tagcccggta gtgccagtaa taatcggttc gaaagaggaa ggtttacgca
     3961 tctggcgcga gcttatctcg ccgggcgtgt atgtgaatct tatcctgccg cctgccgcgc
     4021 ctgcaggcat cacgctgctg cgctgtagcg taaatgctgc ccacagtcgt gagcaaatcg
     4081 atgccatcat tcaggcattt gctactctga agcagtaatg tgagcccgcc gtggtgtttg
     4141 cgccacggcg ggtttaatgt tgtctgtcat accaatacac caggctcaac gccaccaaca
     4201 caaacgctac cggtaaaccg agcgcggtaa caggtgcgct taacgcaaat agcaatccca
     4261 gattaaccat gatttgatag ctgatccagg tgagtaagcc cacgatgacg cctacagcga
     4321 gacggctacc catcccaggc gaacgcgggg cactaaaggt aaacggcaca gccagcaaaa
     4381 tcatcgccag ggtcaggatg ggttgcccca gtttttccca cagtgcaagg tggtattcgc
     4441 tgctgggttg cccggtttcc tgcaaataat ggatgtaatg attaagctga cggacagaaa
     4501 aagtattccc cggcattgat aactcttcaa gattcatact ggtgaaggcc gattgccagg
     4561 caagattatc taatgtttcc agcgtttctt tgccattcag ccatttttta tgattcacac
     4621 catgcaggac ccacgttttg tcgtcttcaa tggtggcact gcgtgcataa atgtaggatt
     4681 caagcgagag atcgtcgcga taatgaaata tctccacgcc caccggctgg ccctgctcgt
     4741 tcagcgattt caccgtcaca aattcattac cacgcctggc ccacagcata ttgccggtaa
     4801 tgtcatcgtc ctcccccaac gcggtggcgg tcgatttgat ttgcaacgcc tgttgctgca
     4861 atggcgacgc cacccactca tctatcgcgc ctaacgaaac agtccacaat atcccggcaa
     4921 ccagcgccac cagtgcaata cggaagatag aaaaccccgt gctgcgaatg gcggtaagct
     4981 cactgttttt cgataactgc cccagtccaa caataccacc caataacgcg ataaacggcg
     5041 aaagttcgac cagtgtgcgc ggtaaggtca ttagcaccac cagcaccgcc tgagtccagc
     5101 gataaccgcc cgggctgaca ccatccagtt cgttaatcag gttgaaggtg gtaaaaagcg
     5161 gcagcaatag ccctgcagcg gcggcaaaac cgagaaagag atgacggatt aaatagcgac
     5221 tgaaaacatt catcgggaaa atttctgcag caaagagaag tcacgtgcga ccagtatcag
     5281 caggcccatt agcatgagtc cgggcactaa ccacaaacca ggaatgaggg gaatcgcgcc
     5341 gttagccacc agtgtacggc agacgtcgcc gccataaaaa atggcaataa acagcaacgt
     5401 cagtggcaat aacgttgaaa atcgcccttg ccgcggtttt acccgactta atgaaatggc
     5461 cagcagcgcc atcaacaatg cggtcaggcc acggctttgt cgccattgca gttcggcatg
     5521 atcggcagga aaggcggagc gcgccagctc cgtgaccgat ttcgctttgc gtttgacgtt
     5581 cgggctttga tccagcggat tcagatgtaa ttgcagatta cggtagatct gttcgttgtc
     5641 gtcacgcccc tgatgatcga gaagataggc agtgccgttg tgcaacataa cggtcggttt
     5701 ctcgggtgat gggtcaacca catcaaccga accggcgcgg aaaatacggg ttcgattggc
     5761 agtagaagta taaatcagcg cgtcagtcag gcgattatta tcctgatcaa ccgtctgcga
     5821 aaggatcatt cgtccgttat cgttagtgtt aaatttcttt gcccgcaact ggcgaacatc
     5881 cagctccgac tgcgactgct gctccagttg ataaatctgc gcataagccc acggacgacc
     5941 atacatcgac agaagggtga caaaaatact tagcggtatc gccagataaa gaacggcttt
     6001 gtacaagcgt cccggactgc cccccgccgc agagatagcg gtaatttccg agtcggtata
     6061 catctgccct agcgtcacgc caactgacac atacagacca acaggtaaca acatctccag
     6121 tgcaatcagt actttgtaga aaacgatatc cagcacaaca tcaagagcta atgtcccatt
     6181 tgccgcttcg gtcagataac gctgtgcgga gtagctggcg aaaataaaga tcagaaaacc
     6241 gacgataatc agcaccagac ggcgcgtacc acgcatgatg tagtgttcaa ccagcttcat
     6301 ctcactaacg ccctcaatcc tttgagtttt ttattcattg atcagtttct gcgccaacgc
     6361 aatgacatga tctacctctt ccggcgtcag gctgccgtcc tttgcccact gaatttgccc
     6421 ggctttattc agcaccagaa tggttgaact ctgctccggc aagcgccagg caacgcgtcc
     6481 ctgtccattg ccgtcaataa cgaattgcgc ccacggatag cggcgtttat ttttttcgat
     6541 tttgccaaca acaaaatagc cagtaccaaa gatggcatca tcggtgttca caatggtggt
     6601 gggctgaaaa cgatcctgcg gaaagtttgc cgcttcaacc gctttaatta acagtgagtt
     6661 ctttttcttc gccgacttac gcccggcgat atactgaata atgcggactt ttcccgctaa
     6721 ttgtgtgctg ttccactttt ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc
     6781 gccttcctgg atataaacag gggtaaccgt tttgccagtg acgaaattgt gtgcctgcac
     6841 acccggggct aagagcatga taatcaacgc aataatcaaa cgagatgaca tcattgctcc
     6901 ggctatagcg gaaaacgcga aatcagcagc tctaagcgct gacaaatatc gctatccagc
     6961 gccaacggtg cataatacga ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt
     7021 ttatctccca tcaggccatt aatggcggcg aggaaacggg aaatctgtaa cgcctcttta
     7081 ttttcaggat caagcgatct taaggtgtcg taataagaaa cctgctggct gatgattttt
     7141 cgcacatcat gaaattccct gccggttagt cgcggtttgg caataatctc tttcagggta
     7201 tgcatttgct tttgccgata ggcaataaaa ctgcttacgc tttctaactg gctatggcgt
     7261 aatgaatagt tcaccaacga ccagaccgga gagcttaaat aaatccgtaa caggttgccg
     7321 taatacgaca caatattttt cttaccgtta cgaaagccgt cctgaaaacg ccccagaaaa
     7381 acggtcgttt tgccgaataa aaatccggcc tgatgcttct ttaaatacag ccgttgcgca
     7441 aaacgcaggt gcttgtagcg cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc
     7501 agctcatcgc ctttggcttg tttgcgaact aaacgcagta attcctgacg atcaacgcca
     7561 ttgagccaga attgcaggca taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt
     7621 agtgcaatag tcggcggcaa actaacctgt gcatcaacga catcatcttc ttcaatcgca
     7681 acaaatagac gctcgctcgc ctcaagcggg aaatattttc tcatcatcag gatttctctc
     7741 cggctgcgtt ataaagcaag gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg
     7801 agagcgccac gtccagacat tgcccaccga agtaagcaaa cgctgctccc agcacaccaa
     7861 acagatgaca gaacatgaac agcaatacga tgtagccgat tgaggcgatg gtttgcgcca
     7921 cgagaaaagc gcgctgtttt cccgccatca atagcaatga ctcctgcggg aagcccagca
     7981 tcgaaatcac aattgcgccc agcatcacct gaatcaggtc atacgcttcg agatacttaa
     8041 cgccaaacac cagcgaaatg agcggcttgc cgacgatcaa caccgcgagc gccaccagaa
     8101 taccgattcc gcccgccagt aaaccggact tcacacctaa caaccacggt ctggtggtgc
     8161 gcggatctaa acgcatcacc tctgggtaaa aacttttacc cagcaaaccc gctggcgttc
     8221 cggcggcgtc gaagaatgtc atggcgattt taaataaccc ggcggcagcg ggtcctaaca
     8281 cgatccccac caacactgtg ctgcacgagt tacgcgccga ccagatggag tgggcaatgt
     8341 tggttgacca gacaaaactc cacgcgcctt taatgtgtcg ggcagactca aacagattca
     8401 atttgaaggc gttatggata tttctacggc gtaattcgcg cgcggcaaac caccagtaca
     8461 tggtgccgcc aaccagattc gacacgtacc aggcaataac aaaacccaca aaaccaaagt
     8521 caaaatacca ggctgcgacg ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta
     8581 cagcaatcaa atcaaagcga tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca
     8641 ttgaaggaat gagcgtgcaa tacagcgccg ccagccaaaa gctttggtca tccagaccta
     8701 atgaatggga aaggaatggc agtaaggcaa tgccgccgac aatcgccacc gcgccgctga
     8761 cgatatccag cgagaaggaa aatgagacga cattgcgaaa ttgctgcgga ttattgttgg
     8821 tcaaggctgg cgttccgtac tgaaccacca gctgccatgt ctgaaactta ataaaatcgc
     8881 tgatcgactt ggcgtacgat tgcacaatca ccagtacacc aaacatggcg ggcgtcatcc
     8941 ctttaccggc acacgagagc gccaacagcc ccagcaaggc gctctcgaca ttactggagc
     9001 ctaaccaggc gctattgcga ataatggtgc gaaacgcgcc atctgcaaac caatgtttga
     9061 tgttaaaacc cgccaattca gcctgacctt atcgttgata gtaaaaaata tcccgccagc
     9121 cttaagttaa acttcggcgg tcagaaacga tggcaaccag agaaaccgcc ttctgtacct
     9181 gttccagcac ttcgctgtag ggcgctctgg aatcaatctc aagaattttt gtgccgttat
     9241 agccaatcgt cgacatgacg ccgattttgt cctgcagctc ggcatactca tggtcaggct
     9301 tgcgggagat ggcagtatca atatcaatcc ccaaacgaat gattaattca ggacggtatt
     9361 gcgccatttt ttggtataaa ctccgttcgc gctgcgccag aaacatgctg attttcccgg
     9421 tcgcacgttc gacgccgatc cccggtccgt cataataaaa gcccgaaatt tcagcctgcg
     9481 ggaagcgatc gctgaccacc agaacgccac tttgcgccag tcgctgaacc ttgcgtagat
     9541 tcgccattct tcgcagcgag aagcagtaca taatcaccgc cgcccatagc gccggagatt
     9601 tggttttcat gctttgggtt ttcgaggatt tggccgccaa tcgccgttcc agccagacgc
     9661 caaccaacgg caatcgtttg attttgtcgc cgtcttcgcc ggagagcagc cccagatagc
     9721 ggcgctcggt ttgccagtgt tgttgcagcg atttcaccag gtcggtggtc agcgtggatt
     9781 taccggtgcc atcacacccg accaccgcaa tcagccccgg aatgtagttg ggctgcggcg
     9841 cggttgtact attaacagtt tgtgtttgta gtgcatccat tcgttaaatc atcccctatc
     9901 cattttggtg atcaggaaag cgcggcgtga attgcgcgta gtgacgcttg cataatttca
     9961 tcggctggtt gccgaccatc cagttcaagg atcttggcgc cattgaatgt cagttgcggc
    10021 gtaacggcga ttttttcctg taacgctgcc agttggtggt caggtttacg cgcaaacgcg
    10081 gtttgttcat caatgccaag acgaatcaac aatacgggta aataagacgc catccattgg
    10141 tacagcttca actcgcgctg ccttaacatt tttatccaac cgttaccgcc cgtggttttt
    10201 gccaattgcg ggccatcaaa gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg
    10261 atgagcagat agccttgctg gcttttacac aacattttgc gaaacttgta cgcccgccag
    10321 caggaaagca gatagataac cagcgcagta atattgccag gcggagttaa gggcttttcg
    10381 tgtacatgtg ccgctttact tcgcagataa cacccacaag gtgcgccaat aacagggagc
    10441 tgtgaaatcc attcgccaat tcgcccggac gattgcccga gataaatgtg ttctgttggc
    10501 attcttgctg ccagttcatt taccaggctt gccgtgaggg tcgatttacc tgaaccatca
    10561 catcctacaa tggcaataac acgcactggc gtggaattaa ttattgacat attacgttga
    10621 ttcacgaaaa acccggcagt aaataatgta ttgaatatta cgtggtccgt gctcagaata
    10681 tccgttcaac cttattgtga caatgactaa ttgtgacatt gacctgaatt acgctttcat
    10741 aaaaacatat taaccaaata aatattttta atggatattt aaattaaagg atatattcat
    10801 gcagtcaata acacctccat taattgccgt tattggtagc gatgcttcag gcaagtcaac
    10861 ggtgtgtgaa catcttatta ccgttgtcga aaaatatggt gctgccgaaa gagttcattt
    10921 aggaaaacag gccggaaatg tcggtcgtgc agtgacaaaa ttacctttga tgggaaaatc
    10981 cttacataaa acaattgaac gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt
    11041 tccggcgctg gtaattacag cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct
    11101 tgcctgtcgt cgtcgcgggt taattgttct aaccgaccgt tatcctcagg accaaattcc
    11161 tggcgcttac gatggtacgg tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg
    11221 gctggcaagc caggaacgta aagcgtttca ctggatggcg agccataagc ctgatctggt
    11281 catcaaactc aatgttgacc ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga
    11341 atcgctggcg aggaagattg ccataacgcc acagttaacc tttggtggtg cacaactggt
    11401 tgatatcgat gccaatcagc cactggaaca ggtgttggtt gatgcagaaa aagcgattac
    11461 ggattttatg accgcgcgtg gttatcacta gtcaaaaatg gaaatgcccg atcgccagga
    11521 ccgggcattt tcaggaaggt taaatcaact gcaatgctat ccagtacagc ccaccagaaa
    11581 gaaaaattgc cgccggtaaa gtaaacaccc acgccatcag gatgctggtt accgttttac
    11641 gctgtaaccc accgccgtcc accaccatcg tccctgctac cgcagacgag aggacgtgtg
    11701 ttgtggagac aggcatccca atataactgg caaggccgat agacactgcc gccgtcattt
    11761 gtgccgccat gccttgcgca tacgtcatgc cgcgcttacc aatcttctca ccgatggtca
    11821 tcgctacacg acgccagcca atcatggtgc caatgccgag cgccagtgct accgccatga
    11881 tgatccacac cggagcgtac tcaatggtgc ttaacatatc gctgcgcagt tttttcagca
    11941 ggttctggtc ttctttactg acgcctggca gtttcgccag cttcgcggag gtatcggaga
    12001 tgcacagcat aatgcggcgc agctggctgc gctgactcac gcttaacggc tcgtaacttt
    12061 ccatattgcc tggcagcatc gttttaacgc gcgcaatcgc atcaaaggta tttgccggat
    12121 gacagtgaaa ctctgttact tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca
    12181 tcgcaatcaa cttctgcggc agttcaggat gctgttgcag gtagtgttcg aagttggtaa
    12241 cggcatcgcg ggtacgggta atttcatagc cggacgcatt catattgacg acgaagccag
    12301 caggggcaat ccccaccagt accagcatta ccaggccgat ccctttctgc ccgtcgttcg
    12361 cgccgtgcga aaacgccacg cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg
    12421 gcggtttacg tttgcccttt ttctttttgc gatcttccgg aatgcggtga atacggtcac
    12481 gctttttcgt cccactccag aaacggcgca gcaggaaaat cagaccaccc gcaatgacca
    12541 ggccgacgat aggggaaaca atcagcgagg agaaaatttt ggttacttca cgcaggttta
    12601 acgcatccat caccgatgag ccggttaaca gcgcgttggt taaaccgatg ccgataatcg
    12661 caccaatcaa ggtgtgcgaa ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc
    12721 agataatcgc cgccagcagc atggaaaaga ccatcgccaa accgtgagtt gaccccatat
    12781 tcagcaacaa atcgattggt aacatatgga caatggcata ggcaacgcta agtccgccca
    12841 ataacacgcc aaaaaagtta aaaaatgccg ccatcaccac agcaagttgt ggctgcatgg
    12901 cacgagtata aataacggtt gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt
    12961 cgtagaacaa cacaaatgcc agagcaagta ataacaaaag ccccgtatat atatcaaggc
    13021 caacaaataa atttagcata tattagatta cgccattttg aatttacgaa cggacgcatt
    13081 atcagtgact ttaacggcat gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg
    13141 ttatttcttg tgtatcagac aataagcatt cattcaaaac ctttagaaat aatcaaaaca
    13201 caggtattcc actggttaat atattcacat atgaaatgaa taaatattgg aatatataaa
    13261 tattgaatat tttgattaat ccccgtacta attattcttc ataatcagta cggggttgct
    13321 acaacatgta ttactttttc accacaatta acggttcaat atcactctct tttttgatga
    13381 ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac
    13441 atacctgaat gtatttaccg tccactttcg gcaaacacca cagttgctga tagatgtttt
    13501 tctgctcggc aaatttaccg ctggttttgt ccagcacctc ttcatgatgg ctgaccaggt
    13561 cgatattgct accacagcga ccggcgatcg gtttcactgc gtaacctgtt ttcaccaatt
    13621 catcattaac agtgaaatcg gtatccagca ggtaacgatg gtgcgggaac agcgaccaga
    13681 ggatcggcag aatcgctttg ttgccgggga tcaccgtcca cagcggctca aagaccagca
    13741 cttccgggcg cagcaatacg tcgataagac gcacttcgtt ttgcggatga ccggtacgga
    13801 ttggcaccgc agcaaactca cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc
    13861 acgcccaggt tttccacacg cagttaacca gtcgcccttc cccatcaatc agttgcccgg
    13921 cagcatccca gcccagttca tccagcccgc gcaagatacg cgtttcaaag cccgcctggt
    13981 gcagcgcctg ctccataaac tgcgcgtgat agttttcctc gatatctttg tcctgcatga
    14041 tatggacaaa cggacgtgca cgactgtgtt tccaggcacc agccagttcg ttaatcagcc
    14101 cttccgccgg attgaagccg ttgcctttat agccctgctc cgcccaacgt tcgaggatca
    14161 agcccgcttc ggtatgacag gaggcggagt cggcgttgta ttcgtaaacc ttcaggccac
    14221 gctcatccat gcagaaatcc atacgaccag tgatcatatg gtgacggcga cgctgccagg
    14281 agagacgcaa acgtggccag aggattttcg gaatgtcgaa cagcgccagc aggttgtcat
    14341 ctttcagcac cttgtcggtt gcgtgaagat acatcagatg cagctcgttg gtggctttaa
    14401 ttagctcctg ctcggcactc tcggtaatgg tgtagtaatg atacggatcc tgattgatca
    14461 cctgaccgtt ggcctgcaca taggcgtttt gtagcggatc tttttcatcc agccatttac
    14521 cgtcaaactg gcctttgttt tccagacgcg ctccactgat tttcagcagc tcgcctgcaa
    14581 tttccggctg cggtaagctg tattcagtat cttccgtctg gatcatccag cccagaatgg
    14641 tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt ttcgaccacc atctccagct
    14701 cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat cacgttctgt tccgcaatac
    14761 ggactttgtt gccatgcaat tgggtaatga tggcgacatg gccagtgtct ttaaattcac
    14821 cgcctttatc ccagataaga agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa
    14881 atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa
    14941 tctcccacgc catacccacg tcagtaaaga ccacaccgta attcagaaag agaaaacggc
    15001 gagcaaattc aacgcattgc cacttgtggc ccatatattc gtcgtcgata tagctacgga
    15061 atacggcgtc atcttcgtat tcctgcggat cgagagaact gtaatctgaa gagtagattg
    15121 ctaccccacc tggggcgtag cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc
    15181 ctttgctcat cactttacct ttaacaatac aacctaagca gttggcgtaa ttgttgtgct
    15241 ctgattacct gctcagcgac attaaccgga cagaggtcag actaatcata cacctcatcg
    15301 cagcggctgg cgcaaagggt taaaaaattc acattctgta cagcaagtga cctgctacac
    15361 tgcttcaaca ctaccactca gaaggcaact cactatgaca gacaatactt atcagcccgc
    15421 gaaagtctgg acgtgggata aatccgctgg cggcgcgttc gccaatatca atcgcccgtt
    15481 ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa catccattgc aactttattc
    15541 gctgggaacg ccgaacggtc agaaagtaac gattatgctt gaggagctac tggcgctggg
    15601 cgttactggt gcagagtacg acgcctggct gattcgtatt ggcgatggcg atcaattctc
    15661 cagcggcttt gtcgaagtga acccaaactc gaagatcccg gcgctgcgcg atcatacgca
    15721 taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt
    15781 tggctacttc ctgccgcagg atctggcaaa acggactgaa acgttgaact ggctgttctg
    15841 gttacagggt gccgcgccgt tcctcggcgg tggttttggt cacttttacc attacgcgcc
    15901 ggtgaaaatt gagtacgcca tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt
    15961 gctggataag caactggcgc agcataagtt tgttgcgggc gatgagtaca ccattgcgga
    16021 tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc ggcgtgtatg atgccgcaga
    16081 gtttctggat gcgggaagtt ataaacatgt gcaacgctgg gcgaaagaag tgggcgaacg
    16141 tccggcagtg aaacgtgggc gtattgttaa ccgcaccaac gggccgctga acgagcagtt
    16201 gcatgagcgc catgacgcca gtgattttga gacaaataca gaagataagc gtcaggggta
    16261 agggttggtg ttcgtcgcag caagccatcc aggccggata aggcgttcac gccgcatccg
    16321 gcaatcgtgc acaatgcctg atgcgacgct gccgcgtctt atcaggccta caaaagctaa
    16381 tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt gcgtctaatg tggctttggc
    16441 ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt accagcaccc actggcccag
    16501 cagatcggca gggttacctt cgcaaatcag ggcgatattc acgtcgcgct tgataccaca
    16561 tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca gccagcacct ggcctggaac
    16621 gccaatacac attgttaact ccggttattt cgattgccgg acaaacgcga aaaagaacct
    16681 ttagcgggtg ttccctctca ccctaaccct ctccccggag gggcgagggg accgtccgtg
    16741 cgctttttag gcgttgtcct cagtcttatt caacttcaat acttttgacg atcagcgaat
    16801 cgccggtatc gacacgcaac cgctcgccgt gacagagcgg acactgcgca tcgtgctgat
    16861 gaatctccac cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat
    16921 gtaaatcgca cccttgcgcc accgttccgt ggcagacaat ttcaaaacta aaacggacgg
    16981 cgctctcctc aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa
    17041 catcgtgctg ctccgcctgc cgttggataa tttcaacggc gctctggcaa agcgacaact
    17101 catgcatttt cgcccctcct gcggccaaac agcagggcgc gacgccctgc gtgtggtaca
    17161 tccggattgg tgactggcaa cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg
    17221 ccttcttcaa tcgacatgct gtgcgaaagc ggcgacatca gcgaacagga gaggtattgc
    17281 gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca
    17341 attttttcac tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca
    17401 ctcagcatcc acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca
    17461 gaaacataca ccggcattga aggatgcaga aaagagagat cgtgcatcga acgccgggca
    17521 atttcttcaa acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct
    17581 tcagtcatga atcgcctccc gtgggatggc ttccacgcct gattcgcgca gcgcagccag
    17641 aacctgctca agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg
    17701 ctccagcgat tccgggatca cgccgaccag ggtcagcttt ttcggaaact caccggtgaa
    17761 gcgcagggcc gacaatacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa
    17821 caacgccgga acttcttcat cccgcaggat catcatcgtt cccggagtgt tctttttcga
    17881 cacaatggca tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat
    17941 tcccgccgtg ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa
    18001 cgcttcgaca atccgcacgc cgatggcttc atcggtcagc aaaatattgc cgacccctaa
    18061 gactaaaata cgcattacag aaccttcact gaaaccactt cgttgccgtc ggcatccact
    18121 acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc
    18181 ggtttattcg gatcggcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca
    18241 tcattgaagt tacgcggacc agagttccag gttgatggaa caaccgcctg gtagttgctg
    18301 atgataccgt ctttaataac catccagtga gagagcatac cgcgcggcgc ttcgaggaag
    18361 ccaacacctt tgaactcacc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg
    18421 cctttgccga tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattca
    18481 cagcagtgaa cggtacgacc aataatacgg cccagcgtgg agtgcagctg tgccacttcc
    18541 agcgtgttgc cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta
    18601 gattcgcgac ctgccgccag tttcaccagc atattagcca gcggacccac ttctaccgtt
    18661 ttgccgtaga aagtcggtga tttcacccag gaatatttcc cgtcgtcaga ccaaccatca
    18721 taagccggaa tggtggtacc ttcccacggc gcctgtggcg cttcgtcttt ataccaggag
    18781 tgcttcgcgc tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga
    18841 cgatacgagg acagatccgc attctcaatg tagcctcccg ggaacaggaa gctgccgttt
    18901 ttgctgtcgg tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc
    18961 agccattccg ggtagaacgc ggcgataacc gcggtatcaa ccttataaac ctgctcaaca
    19021 aagtcgctca gtttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcaacacg
    19081 cccaacccgt cgaggttgat tgggttcgcg acaccaccca cagccaggtt ctgaatgtgc
    19141 ggcgttttac cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc
    19201 tgcaggtagt gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgccgggtga
    19261 ccccagtagc cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg
    19321 ttctgaactt tagtgaactc ttccggactg ttcagatgcc aggtcgaaac gcctttcagc
    19381 atttcggagg ctttggttgg gtcagcttgc agtgcagaag tgatgtccac ccagtccagc
    19441 gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg
    19501 ttacggatgt attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga
    19561 acggaagaca gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat
    19621 gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa
    19681 gcccatgctt tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca
    19741 atacgggtta ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat
    19801 tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag
    19861 cgcaaatctc aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt
    19921 aggcgtaacc gccgcccggg ttgaatgcca ccaacgaata ggtcagacgc caggttgcac
    19981 aacctaacag tgcgctcagt gctgacagga acagcatacg ggaatcatta cgcagcttcg
    20041 ccacacgcag aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg
    20101 agtagaagtc accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc
    20161 gcagcccgat gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaacaggc
    20221 tcttttcatc cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga
    20281 caatcgagaa gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc
    20341 tctgccagag cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact
    20401 ggtgcatggt cggcagcagc gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac
    20461 gttgtagcga caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca
    20521 tcacgccgat gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga
    20581 agtgacccgg aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg
    20641 acaagccacc cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat
    20701 ggtattgccc acggttaaag acgtataccg cccacgccag cgcccagccg ccacaggcaa
    20761 agccggtgcc aatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca
    20821 ggtcagagac agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga
    20881 cgattaacgg tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg
    20941 gatcatgact catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc
    21001 agcacggtta agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt
    21061 tgaatatttt cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt
    21121 tcataaggca cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac
    21181 agatgcggat aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga
    21241 cgcggatagt ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc
    21301 agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc acacttctac gcagccaggc
    21361 agaccgcctt tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc
    21421 gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag
    21481 taacggcaac cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt
    21541 ttcagtgcag agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt
    21601 ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc
    21661 cacacctgaa tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt
    21721 tccccttgcg ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctgg
    21781 cagcctacgc acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg
    21841 ttttcagcag ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag
    21901 gctgctttaa taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt
    21961 tcttttgctg acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa
    22021 ccacaccgcc gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa
    22081 catccggttt ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga tggatgcctt
    22141 tatggaagcc gatacctttt tcgttacagc catagcacgg gtgaccaatc gccaccggcc
    22201 acacaccgcc aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt
    22261 tacagccgag gtggtacaag caccagcctt cgcggtggcc ttcatcaccg aactctttgg
    22321 caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat
    22381 aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt
    22441 gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg
    22501 ttttgcctgg cagaacttct tgcaagctga ctgcgccagt tgggttaact ccagctgcgg
    22561 caacaccgcc ccacgcggag caggaaccaa tagcgataat ggctgctgag ccttccgccg
    22621 ctttgcggat gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt
    22681 ttaatgggat ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt
    22741 tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatattcca
    22801 gagagatagt ctccagcacg aggttttcta ccgttggatg cgttgcacga agcagagatt
    22861 ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag
    22921 taaccgattc ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg
    22981 ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat
    23041 ctccagtcat ttatagttat tccgttgcga gaacctggca tatattttgc ctcaatcgca
    23101 aaatcaataa tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat
    23161 gatactgcgg cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa
    23221 gataatacgt atgtttgatc aattttcgag gcgataaccg gccataaaaa agcgcaacgt
    23281 agaacaggaa ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg
    23341 gcgcgtcaga atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat
    23401 cctgtgtgag tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt
    23461 aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt
    23521 attcatcgtt cacctcattc attatatatc gccatatata acgatatttt aggcaaagaa
    23581 cgacttttgc gcacgctatt ttttcatact tgtttaagac gtaatcccgt aagagataaa
    23641 cctatcagga gagatattta tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg
    23701 cagatttgtc ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga
    23761 tataagcctc ataaacctta ttattgtctt tcaacgccgc ctcgtaagga ggccagccct
    23821 cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca
    23881 catcggcagt gggtgcctga cgaccataaa acggcaccgc gcaggccagt tccggatacg
    23941 cgacagccgc cgcgttcgat acgccaccgc catagcaaaa tccggtaata cccactttac
    24001 ctgtcgcttg cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt
    24061 tggttggatc aacctgctgt tgcagctcac gacctttatc atcatttccc ggataacctc
    24121 caacggaact taatccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg
    24181 ccacatcttc gatatacgga ttcagtccac gattctcatg caccaccccc acggctggcg
    24241 ttttgccgct catttttgcg ggtttcacca gataaccccg tacctcgccg tgaccatttg
    24301 gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc ggtaaactct acctgagtcg
    24361 ccagcgcata atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg
    24421 catacttcgc cgcaaggttg aggaactcac gtttcgaaat tttcccgtga acgtaatagt
    24481 cgtagtaatc caacaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcat
    24541 tcctcaactg gtgttttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
    24601 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
    24661 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
    24721 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcat tatcgctcgg
    24781 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa
    24841 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
    24901 aagcgcagaa gagaactttg gtcgcctgct gcgcgaggat tttgctgctt atcgcgatga
    24961 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccgtacg gctctggcgg
    25021 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
    25081 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
    25141 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
    25201 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
    25261 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
    25321 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
    25381 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
    25441 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
    25501 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
    25561 agatgatcgc gtgacgtcgg tattgattgg tgccagccgc gcggagcaac tagaggagaa
    25621 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
    25681 tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc gataaatgac ctgttaataa
    25741 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
    25801 agcccatcac aaatgcagaa agcacaaacg tcagatggat aatgacgtac cacatcagtt
    25861 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
    25921 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccact
    25981 tcagcttctc tttgttctcg gagatatcca gctgtgagac gaaattctca taaccggaaa
    26041 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
    26101 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
    26161 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
    26221 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
    26281 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
    26341 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
    26401 cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc
    26461 atttaaaaga cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
    26521 cgccaggcat ccaggcgaag atgacaccga taccggattg cggcgagaaa acctatgttg
    26581 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
    26641 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
    26701 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
    26761 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
    26821 acaaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc
    26881 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
    26941 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtgtcatca
    27001 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
    27061 aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
    27121 gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa
    27181 cgcaggataa gatcccgcag tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg
    27241 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
    27301 aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
    27361 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
    27421 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
    27481 atcaggcatt gagcacctaa tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg
    27541 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
    27601 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
    27661 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc
    27721 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
    27781 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
    27841 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
    27901 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
    27961 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
    28021 aagatgcatt gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
    28081 cgcagcggcg ctggcttcca gatctaggtc acggctttgc agcaacaata cctgcgctgc
    28141 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
    28201 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
    28261 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
    28321 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
    28381 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
    28441 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
    28501 ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
    28561 gttggcctgg tttaaggaac gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg
    28621 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaggtga ctacagaggc
    28681 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtactgat acatacccca
    28741 gacggaaagg tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca
    28801 caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat
    28861 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
    28921 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
    28981 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
    29041 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa
    29101 atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg
    29161 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
    29221 tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
    29281 gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt
    29341 tgatgaccaa caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
    29401 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
    29461 agccaaacac taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg
    29521 acgttccggc gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg
    29581 acaacacctg ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta
    29641 ttcaggccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
    29701 gcaatgcccg ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg
    29761 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
    29821 aacatcatga aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc
    29881 gagttaacca ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta
    29941 caggcagcag cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg
    30001 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
    30061 cgttgatcct ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
    30121 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
    30181 ccgatctgga cgccggtttt gcacggattg tataacattg ccacttttgg acaattttgc
    30241 agacatttta ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga
    30301 ttcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
    30361 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
    30421 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
    30481 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
    30541 caaggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc
    30601 tgctggctaa gttatattca ggggagctgg ttagggaata ccaaaacggt gaaaggctgg
    30661 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
    30721 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
    30781 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
    30841 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
    30901 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
    30961 ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
    31021 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
    31081 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
    31141 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
    31201 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
    31261 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
    31321 ctcgacgctc aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag
    31381 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
    31441 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
    31501 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
    31561 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
    31621 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
    31681 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
    31741 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
    31801 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg
    31861 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
    31921 cgccagcaag cgacaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
    31981 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
    32041 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
    32101 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
    32161 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
    32221 aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg
    32281 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
    32341 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
    32401 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
    32461 cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
    32521 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
    32581 atccaacgca ggcgcggtga tctcccgtaa aaccacaggc gacaagcagg cgttccattc
    32641 tgcacatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
    32701 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
    32761 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat
    32821 cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat
    32881 gtgggcggcg actcaggcgc tgaacggttt gattggcact ggcgtaccgc aggactgggc
    32941 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
    33001 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgag accaagcgcg ctaagctgct
    33061 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
    33121 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
    33181 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
    33241 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
    33301 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
    33361 ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct
    33421 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
    33481 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
    33541 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
    33601 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
    33661 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
    33721 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
    33781 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
    33841 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
    33901 cttcatccgc tgatgcaaca acgccagcta cacgcttgga acgcgacaca caaaatccag
    33961 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
    34021 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
    34081 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    34141 gatgtctggg atttccgtct cgacaaagac gaactcggca aaattgcaaa actcgatcag
    34201 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa ttctcccggt
    34261 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    34321 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    34381 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    34441 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    34501 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    34561 cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    34621 ggcgcttacc ctgaacgaca atcttacact accgggcaat attggctggc tggattatga
    34681 tccggtttgt gattgtcagg attttggcga tctggtgtta gaaagcgtag cgataaccca
    34741 aactgacgtc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    34801 aaagaccaca cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgatgatat
    34861 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    34921 cgctttagct tcgttgcaga aagaacagcc agaatccttt gttgctgaac tctttgaaca
    34981 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    35041 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    35101 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    35161 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    35221 tacgctgacc aatggcaaca atgaagagca agagctggtt ttacagcggc gcgaaggtaa
    35281 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    35341 aaaaaccgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    35401 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    35461 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc aactggcgag caatgtacaa
    35521 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    35581 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt
    35641 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    35701 agttgccggg gatttttttt aacgtccaac cgccgcttta gggcgtttct tcgcaccagc
    35761 attcaccgga cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg
    35821 agtcgccatc ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct ggcgacgagc
    35881 ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag
    35941 atgctttttg cccatcgctt ccagcgcctg gcggattaac ggccagtttg ccggatcgtg
    36001 gtaacgcaac aacgctttat gcaaacgacg ctgtttgtcg cccttcggta cgaagacgcc
    36061 ttcgctctta taaccaatct tcgccagcgg ttttttcccg gtgtaataca tggtggttga
    36121 gttcgccagc ggcgatgggt agaagttctg tacctggtcg agacggaaac gatgcttttt
    36181 cagccacagc gccaaattca ccatatcttc atcacgcgta ccggggtgcg cagagatgaa
    36241 atacgggatc agatactgct ctttacctgc ctgtttcgag taggtatcga acagctcttt
    36301 aaagcggtca tagctgccca tgcccggctt catcatcttc gacaacggcc cttcttcggt
    36361 atgttccgga gcaatcttca gataaccgcc gacgtgatgg gtcgccaact ctttgatata
    36421 gcgcggatct tccacggcga tgtcataacg tacgcctgag gcgataagga tttttttaat
    36481 gcctttcaga tcacgcgcac ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat
    36541 gtgcggacaa atatccggat aaacgcacga caaacggcga caagtttgtt cagcgcgtgg
    36601 cgatttgcag cgcaacatat acatgttggc agttggccca ccgagatcgg aaatcacgcc
    36661 cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca ttaatgatcg aatcttccga
    36721 acggctctga ataatgcgcc cttcgtgctc ggtaatagaa cagaaagaac agccgccaaa
    36781 gcagccgcgc ataatgttga ccgaaaaacg gatcatttcg taagccggaa tacgggcatt
    36841 gccataggcc ggatgtggca cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc
    36901 ggtagaaagc gggatagcag gcgggttgat ccacacatag cggtcaccgt gtttttgcat
    36961 caatgcgcgg gcgcagcctg ggttggtttc gtggtgcaga atgcgcgaag catgggcgta
    37021 cagcacttta tcgcccttca ctttctcgaa agaaggcagc aatacgtagg ttttttccca
    37081 cggtttctgg cgcggtggct gcacgattac agctttcgct tcctgctttt tcggcgcaac
    37141 aggtttgtta tccgcacacg gcaaatcttc gccatacgga tgcgggattg ggtcgatttt
    37201 ccctagggta tcaagacggg tggaatccac accgctccag ccaggcagcg cctcttttac
    37261 gataattgcg gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg
    37321 atgcgctact tccaccagcg gacgttcacc gttaccaaac atcagcatgt cggctttcga
    37381 atctaccaga acggaacggc gcacggtatc ggaccagtaa tcataatgcg cggtacggcg
    37441 caggctagcc tcaataccgc cgaggatcac cggcacatct ttccacgcct ctttacaacg
    37501 ctgggtataa accagcgtgg cgcgatccgg tcgcttaccc gcgacattat ctggcgtgta
    37561 ggcatcatca tgacgtaaac ggcgatcggc ggtatagcgg ttgatcatcg aatccatgtt
    37621 gccagcagta acaccaaaaa acagattcgg tttacccaga cgcataaagt cgtctttgct
    37681 gctccagtcc ggctgggcga tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc
    37741 gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat
    37801 gtcgcagcta tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc
    37861 cggtccaaaa caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat
    37921 cagggagata gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga
    37981 ttataagccg gaacgaaaga gaaatcgaaa ggtattccat actcgccctc ctcgggcgag
    38041 tatgaagatt acggtaccgg attgaccaac agttgcccaa tcgagccacg gtccgccatt
    38101 tccagcgtct gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa
    38161 acaagcagct ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg
    38221 aacggcatcg cgccattcac gttacggatc tggaacatta cgccttcaat atggaacgct
    38281 tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca
    38341 atacggttga cgtcccacag ctgtccatta atacccgggt catcacccag actgatatcg
    38401 cgactgcgaa ttggcgaacc agccatgatt tcagttggca gcaagcgcat cggaagactg
    38461 tctgtgacca gcggcagaag gccggttggg cgcagcgtta gcaccaaggt agaaaccaga
    38521 atactggatg gctcaaagaa gccgcgaata cgatcaacaa tgctcgccgc ttcaccacag
    38581 gtgatcgaca cttcatcgcc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt
    38641 gccagcgaaa gttgcttcac cgatacagga gcaggcagga atccctgatc gccagaaatc
    38701 acatgtaacg ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc
    38761 agcagtcgca agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta
    38821 accagcagcg tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca
    38881 aagttatcca gccgtttatc ctggataatg accggaaaat catccacacc ataatggttg
    38941 gggataggca gcgacttgct gacttcatct tccaccagcc acattccggc aaggccgtta
    39001 tagacctgct gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc
    39061 tggcgaatag gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg
    39121 cccatcagcg ggcctggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg
    39181 cggttgctgt aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg
    39241 taacgaccat tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca
    39301 cgttgtacag tcataaacag cggttgccca cggcgagatt caagtagcgg cggaacgggt
    39361 agcggttgct gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca
    39421 atccccgatg cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact
    39481 ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa
    39541 cttttccggc ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca
    39601 ttatcgaacg acaatgggca gccagctcac gaacctgatc tttgccatac tgactgacgt
    39661 caattggcgg cagcatttcg acaatcacca gaccgttgtg cagtcgatta agattaatct
    39721 tattcgaagt tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt
    39781 gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga
    39841 acatccagat ggaaatacgg cgttttttga aatgattcac gacttccgca atggtgccgt
    39901 gagctttagt gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc
    39961 cgaagaaggg gatccacagc aagctctttt tacctaccgt caccgtcggc ggttgcacaa
    40021 tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt agcgatatag atagcattgc
    40081 cgtagctttc agcatctgca ggtttgcggc actcaacttt caggccgaac agtggcgcaa
    40141 gacggccaaa catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc
    40201 agtaaatgga gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa
    40261 agatatatag cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa
    40321 tcaggttagg gcattgttaa aagccgctct ctggaaaagc gtattgttaa tcgcaacgca
    40381 tgaataaaca acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg
    40441 atgacgactt aacgtttcat ccggcgttcc ttgctagcgg gaggaaacag cgccctcccc
    40501 ggcatattac tcttcgctat caccgctgct ggcacggcga ggagagtcga tctcaacacg
    40561 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcgc cagtgacttt
    40621 ctgtagctct tccggacgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    40681 ttgcggcggc agaacgtaca gttgcgccag accatcctcg ccacgcgcgg cttctgccga
    40741 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg
    40801 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    40861 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacccgcacg
    40921 gttacgcgct accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    40981 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    41041 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    41101 gatggcatag ctacgaccgg tggaatcaac aaacactacc ggttggttac tcttaccttt
    41161 caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    41221 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    41281 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    41341 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    41401 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    41461 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    41521 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    41581 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    41641 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    41701 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    41761 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    41821 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    41881 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    41941 gcgcatttgc gcagcaattt gctccagtac gcgcgcacct gaaacctgat gcggtaatgc
    42001 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg
    42061 tccgttctcg tagattttac gaatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    42121 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtagttttcg gctggtcgat
    42181 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    42241 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacggg caggtagcat
    42301 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    42361 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    42421 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    42481 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    42541 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    42601 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    42661 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    42721 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    42781 aacttattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    42841 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    42901 catcaatgtg ccttgatata caactctttg ctgtagactt gatcggcata gagggcagca
    42961 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    43021 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatacg
    43081 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    43141 ttgctcatgg atatccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    43201 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg acattgttcg
    43261 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgccaac
    43321 ggcacataaa catccgtttc cagtcgctgc tctgtcaccc cttcggcaag attggtgtag
    43381 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    43441 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    43501 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    43561 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg ggcgtcccag
    43621 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    43681 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    43741 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    43801 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    43861 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    43921 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtgat cgatatcaac
    43981 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    44041 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt
    44101 tacgctgccg ttcggcccct gaaatcctcc ggcctgactt tgcgttaccg atgggccaga
    44161 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    44221 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    44281 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    44341 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    44401 actgatagaa gatgacatgc tgattggtga cggcatcaaa actggcctta gtaaaatggg
    44461 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    44521 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    44581 cgaatggcga gagaaaggtc agcgtgagcc ggtactgaac ctgaccgcgc gtgatgcgtt
    44641 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    44701 gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag
    44761 caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc
    44821 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    44881 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    44941 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    45001 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    45061 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    45121 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    45181 acccaactga tgctgtttgc caaacggtta agtacgctcg atctcaacga aatcaacgcg
    45241 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    45301 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    45361 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    45421 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    45481 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    45541 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    45601 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    45661 ccactaaacg cgactggcgt acccagcgaa gtgcggccac tggttgagtc gctaaatcaa
    45721 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    45781 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    45841 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    45901 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    45961 caggacgtcg cggagatccc gcttgaagat ctcctacaat cgtcggtgat ggatatttac
    46021 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cggcatcaaa
    46081 cgcaccggac aaccgctatt gctaagtttg ttggtgcgaa atttgctgga taacggcccc
    46141 ggtgtgacac cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc acccggacaa
    46201 accgctaccg gcagtggact tgggctatcg attgtccagc gaatcgccaa gctgcatgac
    46261 atgaatgttg agtttgggaa tgcggaacaa ggtggatttg aggcgaaggt aagctggtaa
    46321 cttctctgtc tatgagagcc gttaaaacga ctctcataga ttttactaat agcaaaatat
    46381 aaaccgtccc caaaaaagcc accaaccaca aaccagacaa ataacgagcg agaatacata
    46441 tagccaaaac tcgaacattt cccgcataaa gagttttctt aagataagaa taataagtgg
    46501 cgtaagaaga aaaaatgctg caatcattgc ttgcaatata cgatcttcgc ctgaaacgga
    46561 aggtatactg caaatattgc ctcccgactc aagcatccag tcatacttat tgaaagcaaa
    46621 aaaagaatct attaaccaat aagtaagcat tatatatggc aatgcttcaa atattgtttt
    46681 aattttctgc tttaacatat atcagtacgc tcataaaaat ccatttataa ttatcaacat
    46741 gacacgttga tattttcatc ttttacttat tcaaagccat attttcaatc cccaaaaaaa
    46801 ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat tatgaggaat ggctatcagt
    46861 attgtcattt tcagaaaata tttatcctgc atcggtgagt cagagctaga tcagactttt
    46921 gctaaattcg caaaagactt tgcacatttt gctaatttca cagtaccgct ctgtgacgta
    46981 ctatagtcga caaacgtctc accttgagat taaaaaatga gcaacatcct gattatcaac
    47041 ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg
    47101 gatggcacac tgcgcgacct cgggcatgat gtccgcatcg ttcgcgccga cagcgactac
    47161 aatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca
    47221 ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata ttgatgatgt attcaccgaa
    47281 ggtcacggga ctctgtatgc cagcgatggt cgtacccgca aagatccgtc gaaaaaatac
    47341 ggttccggca gcctggtaca ggacaaaaaa tatatgcttt ctctaacctg gaacgcacca
    47401 atggaagcct tcaccgaaaa agatcagttc ttccacggcg ttggcgttga cggtgtgtat
    47461 ctgccgttcc ataaagcaaa ccaattcctc ggtatggaac cgctgccgac atttatcgct
    47521 aatgacgtga taaaaatgcc tgatgttccc cgctatactg aagaatatcg caagcatctt
    47581 gtggaaattt ttggttaact agagctcagg ctttagaagg agttaaccat gcttaccgta
    47641 atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc aggcggtatt ggatcagttt
    47701 gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc acggctatgc gccaatggtg
    47761 gattgcgctg ctggcgtgag tttccagtct atggcaccgg attctatcgt gatgattgag
    47821 cagtgggaaa gcatcgcgca ccttgaagcg catctgcaaa ccccgcacat gaaggcgtac
    47881 agcgaagccg taaaaggcga cgtgctggag atgaatatcc gtattctgca gccagggatt
    47941 taatcctgcc ttgtttgccc ggccatcctg accgggcaat gttctttcct ttaaacctca
    48001 atctccgcca tgtcgccttt ctcttgcaac cagttgcggc gatcttccga gcgtttcttc
    48061 gccagcagca tatccatcat cgcgtcagta cgctgatcgt cttcatcatc gatagtcaac
    48121 tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac gcaattgcat cgggttcatt
    48181 tcccccagac ctttaaaacg ctggacgttc ggcttgcctt tcttgcgttt taattgctca
    48241 agtacgccct ctttctcttc ttccgtcagc gcgtaataaa cctctttccc gagatcaata
    48301 cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt
    48361 tttacgaaca aagcgcagag cagcgtggca atgtgcagac catcagagtc cgcatccgcg
    48421 aggatacaga ttttgccata acgaagctgg ctcagatcgt cgctgtcagg atcgataccg
    48481 atcgctaccg aaatatcatg cacttcctgc gaggccagca cttcgtcgga agaaacttcc
    48541 caggtgttaa ggatcttacc tttcagcggc atgatcgcct gatattcacg atcgcgcgcc
    48601 tgcttggcag atccgcctgc ggagtcacct tccacgagga acagttcggt acggttaagg
    48661 ccctgcgcgg tacaatcagc cagtttgcca ggcagcgccg ggccgctggt cagcttttta
    48721 cgcaccactt ttttagccgc acgcatacga cgctgggcgc tggaaatcgc catctctgcc
    48781 agcaactccg ccgcctgaac gttctggttc agccacagga tgaaggcatc tttcaccacg
    48841 cccgaaacga atgccgcaca ctgacgcgaa gagagacgct ctttggtctg cccggcaaac
    48901 tgcggatcct gcattttaac cgacagaaca taggcgcagc gatcccagat atcttccgcc
    48961 gacagcttca cgccgcgcgg cagaatgttg cggtattcac agaactcacg catcgcgtcc
    49021 aacaggccct gacgcagacc attaacatgg gtaccgccct gcatcgttgg gataaggttg
    49081 acgtagcttt cggtcagcag ttcaccgcct tccggcagcc acagtagcgc ccagtccaca
    49141 gcttcagtat cgccagcgaa attaccgata aacggttttt ctggcagcgt cggtaaaccg
    49201 tttaccgctt ccgccaggta atcattcaga ccgtcctgat agcaccagcg ctgttcggtg
    49261 ttgttgatct catctttaaa ggtaatctca acgccggggc acagtactgc tttggccttc
    49321 agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt caaagaaggt ttcatccggc
    49381 cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc
    49441 tgcacctttt cgccattttc aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta
    49501 acttctacgc gcttcgacaa ggcgttaact accgaaatcc ccacgccatg caggccgcca
    49561 gagaactggt aatttttgtt agagaattta ccgcctgcat gcagacggca aagaatcagt
    49621 tcaaccgccg gtactccttc ttccgggtga atatccaccg gcatcccgcg cccatcgtca
    49681 atcacttcta acgactggtc agcatgtaag attacatcca cgcgttttgc gtgacccgcc
    49741 agtgcttcat cgacactgtt atcaataact tcttgcccca aatggttagg gcgagtggta
    49801 tcggtataca tccccggacg gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca
    49861 tcagcgttat aagtttgcgt catggtttaa gttagcaatt cgagttgatc gtcagagatg
    49921 gtgcagacca agaaaatcga cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg
    49981 gttgccgcct tctatgacag tctggcggca ggaagcatag tacgccaccg cctggcggta
    50041 atccagcact tcatctcccg tctgttgcag cagccagatc aaatccggcg cttccagcgg
    50101 gtcaatctgc atgactttga gatcgtaaat atggcgtgac tctagcacat attgctgccc
    50161 ggtgtagggg ttctcgttct gaccgagata gtccgtcagc aattcaaacg ggcgtaccgc
    50221 cgggtttacc accactgcgg gcagcataaa acattgcgac aaccaggtgg cgtaatatcc
    50281 ccccagtgac gaaccgacaa tacccagcga atcgccgcca tgttccagga caatggattc
    50341 cagcagttct gccgcgtcgg aaggatacgg cggcaactgc ggaatgatca tctcaacgtc
    50401 agggtgatgt tccgccagcc agttttttaa caagctcgct tttgcagagc gcggcgagct
    50461 gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc cttctgaagc ggtatcaggt
    50521 tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac
    50581 tcgagagtac gccagccggg cgcgatagta tccagcgtaa agttggaaca gtgcggctta
    50641 aactgcacac aggtcgacgg cgttgccagc aggcggcgac cgttccagtc gagatccagc
    50701 tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta
    50761 tccagttcgc ccgcgttacg cagactgtgt tgatcgagcc aactacaacc cgcaggtagc
    50821 ggatgatgat gcagcagcag caacgtatgg cgttctggcg catcggccag tttacgttcc
    50881 agccactcaa gctgaaactc gctcagctca ccgtgcggca cgccaaacac ctggctatcc
    50941 agcaacagga tttgccattg ctcaccaata aacacccgct tcgccgggga gatacccgca
    51001 tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt tgcccggcag ccagacgcag
    51061 ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat aggccgcaga ggattgatcc
    51121 tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt gctggtgtgg acgaatcgcc
    51181 tccagcaccg cctggtagct ttcccaggtg tttaccccta acagggcttc gtgcttttgt
    51241 gcaaacaggt gagtatcggt aatttgtaaa atccttcctc tggcctcacc agccagagga
    51301 agggttaaca ggctttccaa atggtgtcct taggtttcac gacgctagta aaccggaatc
    51361 gccatcgctc catgtgctaa acagtatcgc aaccagtccg ctaaaaactg gttaatttga
    51421 tgcttttcgt cgcgttgatg caacttttta ttaggataat cataccgcgc tttgaagcga
    51481 aaaatctgct ggcttgaaca cacttcagcc accatcgcgt catgatacag acgcaccgtc
    51541 attgacggaa ggctccagta actgatcgcg ggcgcagtct gttctattgt caccagggta
    51601 gtgtatcggg tcgattccac aatcgtcagc cgatattgtg cgtttgccac ctgatagctt
    51661 acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc gcaattgtga aaagttcatc
    51721 tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac gcttcattta tgcccactca
    51781 ttttttaacg cttgatgatg cagctgcagc cattgcaaag cgatgaccga cgctgcgttg
    51841 tcgattttcc cctcttctac ccactggtat gcctgttccc ggcttaccac atgaacgcga
    51901 atatcttcgt tttcatcagc cagaccatga ataccgcttg cggtcgtggc gtccacttcg
    51961 cccaccataa ttgacgaacg ctcactggtg ccccccgggc ttgccaggaa acttaacacc
    52021 ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg cttcgcgacg ggcaacatct
    52081 tccacacttt caccctcttc aatcatcccg gcaaccatct ccagtagcca gggggtttcg
    52141 ctggtgtcgt acgcggcaat ccgaatctgc tcaatcagca caacttcatc acgcactggg
    52201 tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga
    52261 ctcatttgcc cgttgaatag acgatgacga aatctataaa gatctaatga aaaaaagccg
    52321 cgataaagtg tttctcgtgc aataatttct acatcgtttt tgccaaatgt aacgggcagg
    52381 ttgtctggct taagcattgt taatgtccta gcactaatag tgaattaaat gtgaatttca
    52441 gcgacgtttg actgccgttt gagcagtcat atgttaaatt gaggcacatt aacgccctat
    52501 ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta gaatccgcaa taattttaca
    52561 gtttgatcgc gctaaatact gcttcaccac aaggaatgca aatgaagaaa ttgctcccca
    52621 ttcttatcgg cctgagcctt tctgggttca gttcgttgag ccaggccgag aacctgatgc
    52681 aagtttatca gcaagcacgc cttagtaacc cggaattgcg taagtctgcc gccgatcgtg
    52741 atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt actgccacag ctaggtttag
    52801 gtgcagatta cacctatagc aacggctacc gcgacgcgaa cggcatcaac tcgaacgcga
    52861 ccagtgcgtc cctgcagtta actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa
    52921 cgctgcagga aaaagcagca gggattcagg acgtcacata tcagaccgat cagcaaacct
    52981 tgatcctcaa caccgcgacc gcttatttca acgtgttgaa tgctattgac gttctttcct
    53041 atacacaggc acaaaaagaa gcgatctacc gtcaattaga tcaaaccacc caacgtttta
    53101 acgtgggcct ggtagcgatc accgacgtgc agaacgcccg cgcgcagtac gataccgtgc
    53161 tggcgaacga agtgaccgca cgtaataacc ttgataacgc ggtagagcag ctgcgccaga
    53221 tcaccggtaa ctactatccg gaactggcgg cgctgaatgt cgaaaacttt aaaaccgaca
    53281 aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac
    53341 aggcacgctt gagccaggac ctggagcgcg agcaaattcg ccaggcgcag gatggtcact
    53401 taccgactct ggatttaacg gcttctaccg ggatttctga cacctcttat agcggttcga
    53461 aaacccgtgg tgccgctggt acccagtatg acgatagcaa tatgggccag aacaaagttg
    53521 gcctgagctt ctcgctgccg atttatcagg gcggaatggt taactcgcag gtgaaacagg
    53581 cacagtacaa ctttgttggt gccagtgagc aactggaaag cgcacatcgt agcgtcgtgc
    53641 aaaccgtacg ttcctccttc aacaacatta atgcttctat cagtagtatt aacgcctaca
    53701 aacaagccgt agtttccgcg caaagctcat tagacgcgat ggaagcgggc tactcggtcg
    53761 gtacgcgtac cattgttgat gtgttggatg cgaccaccac gctgtacaac gccaagcaag
    53821 agctggcgaa tgcgcgttat aactacctga ttaatcagct gaatattaag tcagccctgg
    53881 gtacgttgaa cgagcaggat ttgctggcac tgaacaatgc gctgagcaaa ccggtttcca
    53941 ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa tgctattgct gatggttatg
    54001 cgcctgatag cccggcaccc gtcgttcagc aaacatccgc acgcactacc accagtaacg
    54061 gtcataaccc tttccgtaac tgatgacgac gacggggctt cggccccgtc tgaacgtaag
    54121 gcaacgtaaa gatacgggtt atctgccgca ttcttccccc ttctcgcttc aatttcgacc
    54181 agccatcctc tattctgatg ggtatttacc actggtcccg gaagacaaaa atgaaacgga
    54241 caaaatccat acgccacgca tcgttccgca aaaactggag cgcacgccat ctgacaccag
    54301 tcgctctcgc ggttgccact gtttttatgc tggctggctg tgaaaagagt gatgaaacag
    54361 tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat
    54421 gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg tactgcgccg aaatacgcca
    54481 cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg tcagcaagca ccagcccagg
    54541 ctggcatggc accagaaaac caggcgcagg ctcagcaatc cagcgggagt ttctggatgc
    54601 cgctgatggc cggttacatg atggggcgtc tgatgggcgg cggcgcggga tttgcacagc
    54661 agccgctgtt ctcctcgaaa aacccggcca gtccggctta cggtaaatat accgacgcga
    54721 cgggtaaaaa ctatggcgca gcccagccag gccgcaccat gaccgtaccg aagacggcaa
    54781 tggcaccaaa accggcgacc accactaccg ttacccgtgg cggttttggt gaatctgttg
    54841 ccaaacaaag cactatgcag cgtagcgcaa ccggcacctc ttctcgttca atgggtggct
    54901 gataccgatg gaaagagtca gtattaccga gcgcccggac tggcgtgaga aagcccacga
    54961 atacggtttc aattttcaca ccatgtacgg cgagccgtac tggtgtgaag atgcttacta
    55021 caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc accgccgaac tgcaccagat
    55081 gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag ctgatgacca aattccgcat
    55141 accaaaacac acctggagtt ttgtgcgtca atcatggctg actcaccagc catcgcttta
    55201 ttcgcgtctt gatctggcgt gggatggcac tggcgaacct aaacttctgg aaaataacgc
    55261 cgatacgcca acgtcactat acgaggcggc gttctttcag tggatctggc tggaagatca
    55321 gcttaacgcc ggtaacttgc cggagggcag cgaccagttt aacagtctgc aagaaaaact
    55381 gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc cagttgctgc atctcacctg
    55441 ctgtcgcgac acggtggaag atcgcggaac cattcagtat ttgcaggact gcgcaaccga
    55501 agctgaaatc gccaccgagt tcctctacat cgacgagata ggtttaggcg aaaaaggcca
    55561 gttcacggat ttacaggatc aggtgatttc taacctgttc aagctttatc cgtgggaatt
    55621 tatgctgcgc gaaatgttct ccaccaagct ggaagatgct ggcgtacgct ggctggaacc
    55681 ggcgtggaag agcattatct ccaacaaggc acttctaccg ctactgtggg agatgttccc
    55741 gaatcacccg aacctgctgc ccgcttattt tgcggaagat gatcatccgc aaatggaaaa
    55801 atatgtggtt aaaccgatct tctcccgtga aggcgcaaac gtgtcgatca ttgagaacgg
    55861 caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa gggatgattg ttcagcaatt
    55921 ccacccgtta ccgaaattcg gcgacagcta tatgctgatt ggtagctggc tggtgaacga
    55981 tcaacctgcc ggaattggca ttcgtgaaga ccgtgcgttg atcacccagg atatgtctcg
    56041 gttttatcca catatttttg ttgaataagc cacgataccg gatggcactc gccatccggt
    56101 aattgttagc ctatctgcac cgacagcata cttaaactgc ccatttctat accatcgacc
    56161 ggaatggtaa ttggctcctg cccatcccac acacctaaca catacaacaa cggcagatag
    56221 tgctctggcg ttgggttcga taacgcgcca ccttcatggt cgagataatt caccagagga
    56281 tgctgctcta ccggcccttg ccacgtcaga ttcgctttca catactcatt aaacgacgtc
    56341 gcccacggat acggtgaact atcaccgtgc cacttcactg tgcgcaggtt atgcaccacg
    56401 ttaccgctgg cgaccaacat tattccttca tcacgcagcg ccgcgagttt gcgccccatt
    56461 tcgaaatgcc aggcggcagg tttgctactg tcgatactca actgcaccat agggatatcg
    56521 gcgtcaggat acatcttaat cagcacgccc cacgagccgt ggtcaaagcc ccaggcttct
    56581 ttatccagcg tcaccgggat cggcgctaac agctcaacca gacgctgtgc cagcgcaggc
    56641 gaacccggag caggataatg cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga
    56701 atcgtgggcg gcgtctccat cgcggtcact cctgttccac gggtaaacca gtgagccgaa
    56761 accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca acttctgcca gctgcgggta
    56821 tacaaattat cttccagcac gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata
    56881 cgtgttgaag acatgatgat atccttaact aaaggtgtca ttttgatatc ctcacaatac
    56941 gcttgttcgg cggagtaaga acccggataa caatgatgat gatcatcagt tattttgacg
    57001 atctgcctga aggtgaagat ttataaggag ttgtcgatgt cagtacctct cattctgacc
    57061 atactggcgg gggcagccac gtttattggc gcgtttctcg gcgttctcgg acaaaaaccc
    57121 tcgaaccgct tactggcgtt ttcgctaggt tttgcggcgg ggatcatgtt gctcatctca
    57181 ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa tgtcgccggt gttgggttat
    57241 ggaatgttta tcttcggtct gcttggctac tttggcctgg accgcatgtt gccgcatgcg
    57301 catccgcagg atttaatgca aaaatcggtg cagctgttgc caaaatcgat caagcgcaca
    57361 gccattctgc tcactctcgg catcagtctg cataacttcc cggaagggat tgccaccttt
    57421 gtcacggcga gcagcaacct ggagctggga tttggcatcg cactggccgt cgcgttgcac
    57481 aatatccctg aaggtctggc agtggcaggc ccggtttatg cggcaacggg gtctaaacgc
    57541 accgcgattc tgtgggcggg gatttctgga ctagcagaaa ttcttggtgg tgtgctggcg
    57601 tggttaatcc tcggtagcat gatttccccg gtggtcatgg cggcaatcat ggcggcggtt
    57661 gcaggaatta tggtggcgct ctcggttgat gaattaatgc cgctcgccaa agagattgac
    57721 cctaataata accccagcta tggcgtacta tgtggaatgt cagtgatggg attcagttta
    57781 gtgctgctac aaacggcggg aattggttaa aaacaacatc agaagttgtc cagcacatgg
    57841 acaacttctg ccagcttcac gaaagcctta ttctaccttt tcggctgtct cttctctcgt
    57901 actgtttaag tatttgttcc gcgcttcaag cagtgcgtct ctgtcctggc gaaacgcccg
    57961 acaataacgt ttcaaatgca ggaaataacc caccacaacc agagcaatca atacaatcca
    58021 gtaccaggcg ataaacatcc ttttaccctt aatatttaac aatactaaac atttagcgca
    58081 taaatttcac atatcctttt tcggatatat tcatatggtc gcataataac ggaacaatta
    58141 tatgattttg atcataatga aaaagaatgc ctgatattaa tgaaattttc tcatagaaag
    58201 agaagcggaa ttaacatctt gcatagcagg gcaaaataaa aaccgaagcc agctacaact
    58261 tcggtttctt gattaaggca gtaattaagc agcggttttc agctggcttt acgctcatgt
    58321 gcctgacggt atgccaccag gtcttcaata gtcacgagcg ccatattgtg tttattggca
    58381 aactcaatgc actctggtgc acgcgccatc gtgccatcgt cattagtcag ctcacacagc
    58441 acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa tagtcgcttc agtatgaccg
    58501 ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga aaacgtggcc cgggcgattc
    58561 agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat acgatcagcg
    58621 gcagaaacac cggtagtcac accttcagcc gcttcaatgg tcacggtaaa accggtgcca
    58681 taggcgctgg tgttattttc taccatcatt ggcagatcaa gttgtttacg gcgatcttca
    58741 gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg tcagcgccat ctgctcaacg
    58801 gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt cacggtcttc atcatcaagc
    58861 accattacac cgcgtccttc acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc
    58921 gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa aacctcacta aaattatggt
    58981 taccagaatc agggcagtct taggagtggc ggcatatagc caaaataacg tgagcgggtc
    59041 catgcccgac agaatcgtta ctctctccca tccggactct aaccgtcggc cccggaatta
    59101 caccggatct gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt caactgagtt
    59161 gatttaccgc cggtggggaa tttcgccccg ccctgagaat aagcggattc actataacgc
    59221 taatgattag cggcagcaac gcatagcttc acataattct ggtttatgac ttacccttat
    59281 cgcactacaa tggtactcaa cacctatcag tacagggaaa ctacaatgat tgacccgaaa
    59341 aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc ctaaaggaat cagggagttc
    59401 ggggaagatg tggagaaaaa aatccgccaa accctacaag ctcagctgac gcgcctcgac
    59461 cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc tgttacggac tcgtgagaaa
    59521 ctggcgctgc ttgaacaaag aatcagcgaa ctggaaaatc gcagtactga gatcaaaaag
    59581 cagccagatc cagagactct ccccccaacg ctgtaaacat ttgtctttaa tgcactgtaa
    59641 attatgaatt aatcctctaa tttacagtgt tttctattac attatcgctt aatcccattc
    59701 agagtatcat cacaaaaatg aatgattgca attgaaaata taattgttat gtattcttta
    59761 agttttac
//
LOCUS       DAZOZX010000006        59763 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZOZX010000006 DAZOZX010000000
VERSION     DAZOZX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.006"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            546..1835
                     /gene="sdaC"
                     /locus_tag="ACHUPT_000338"
     CDS             546..1835
                     /gene="sdaC"
                     /locus_tag="ACHUPT_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0818082.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1893..3260
                     /gene="sdaB"
                     /locus_tag="ACHUPT_000339"
     CDS             1893..3260
                     /gene="sdaB"
                     /locus_tag="ACHUPT_000339"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0818083.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3371..4126
                     /gene="xni"
                     /locus_tag="ACHUPT_000340"
     CDS             3371..4126
                     /gene="xni"
                     /locus_tag="ACHUPT_000340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0818084.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4179..5327)
                     /gene="fucO"
                     /locus_tag="ACHUPT_000341"
     CDS             complement(4179..5327)
                     /gene="fucO"
                     /locus_tag="ACHUPT_000341"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0818085.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5355..6002)
                     /gene="fucA"
                     /locus_tag="ACHUPT_000342"
     CDS             complement(5355..6002)
                     /gene="fucA"
                     /locus_tag="ACHUPT_000342"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0818086.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6549..7865
                     /gene="fucP"
                     /locus_tag="ACHUPT_000343"
     CDS             6549..7865
                     /gene="fucP"
                     /locus_tag="ACHUPT_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0818087.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAATPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7898..9673
                     /gene="fucI"
                     /locus_tag="ACHUPT_000344"
     CDS             7898..9673
                     /gene="fucI"
                     /locus_tag="ACHUPT_000344"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR0818088.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9782..11201
                     /gene="fucK"
                     /locus_tag="ACHUPT_000345"
                     /pseudo
     CDS             9782..11201
                     /gene="fucK"
                     /locus_tag="ACHUPT_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11203..11625
                     /gene="fucU"
                     /locus_tag="ACHUPT_000346"
     CDS             11203..11625
                     /gene="fucU"
                     /locus_tag="ACHUPT_000346"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0818089.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11683..12414
                     /gene="fucR"
                     /locus_tag="ACHUPT_000347"
     CDS             11683..12414
                     /gene="fucR"
                     /locus_tag="ACHUPT_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0818090.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12458..13558)
                     /gene="rlmM"
                     /locus_tag="ACHUPT_000348"
     CDS             complement(12458..13558)
                     /gene="rlmM"
                     /locus_tag="ACHUPT_000348"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0818091.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13551..13946)
                     /locus_tag="ACHUPT_000349"
     CDS             complement(13551..13946)
                     /locus_tag="ACHUPT_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0818092.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13965..14882)
                     /gene="gcvA"
                     /locus_tag="ACHUPT_000350"
     CDS             complement(13965..14882)
                     /gene="gcvA"
                     /locus_tag="ACHUPT_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0818093.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15233..15460)
                     /locus_tag="ACHUPT_000351"
     CDS             complement(15233..15460)
                     /locus_tag="ACHUPT_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0818094.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15652..16857
                     /gene="csdA"
                     /locus_tag="ACHUPT_000352"
     CDS             15652..16857
                     /gene="csdA"
                     /locus_tag="ACHUPT_000352"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0818095.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16857..17300
                     /gene="csdE"
                     /locus_tag="ACHUPT_000353"
     CDS             16857..17300
                     /gene="csdE"
                     /locus_tag="ACHUPT_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0818096.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17351..18157)
                     /gene="tcdA"
                     /locus_tag="ACHUPT_000354"
     CDS             complement(17351..18157)
                     /gene="tcdA"
                     /locus_tag="ACHUPT_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0818097.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="ACHUPT_000355"
     CDS             complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="ACHUPT_000355"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0818098.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19701..19777
                     /locus_tag="ACHUPT_000356"
     tRNA            19701..19777
                     /locus_tag="ACHUPT_000356"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19735..19737,aa:Met,seq:cat)
     gene            complement(19852..21105)
                     /gene="amiC"
                     /locus_tag="ACHUPT_000357"
     CDS             complement(19852..21105)
                     /gene="amiC"
                     /locus_tag="ACHUPT_000357"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0818099.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21337..22668
                     /gene="argA"
                     /locus_tag="ACHUPT_000358"
     CDS             21337..22668
                     /gene="argA"
                     /locus_tag="ACHUPT_000358"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0818100.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22730..24556)
                     /gene="recD"
                     /locus_tag="ACHUPT_000359"
     CDS             complement(22730..24556)
                     /gene="recD"
                     /locus_tag="ACHUPT_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0818101.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24556..28098)
                     /gene="recB"
                     /locus_tag="ACHUPT_000360"
     CDS             complement(24556..28098)
                     /gene="recB"
                     /locus_tag="ACHUPT_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0818102.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28091..30979)
                     /gene="ptrA"
                     /locus_tag="ACHUPT_000361"
     CDS             complement(28091..30979)
                     /gene="ptrA"
                     /locus_tag="ACHUPT_000361"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0818103.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31155..34523)
                     /gene="recC"
                     /locus_tag="ACHUPT_000362"
     CDS             complement(31155..34523)
                     /gene="recC"
                     /locus_tag="ACHUPT_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0818104.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34536..34886)
                     /locus_tag="ACHUPT_000363"
     CDS             complement(34536..34886)
                     /locus_tag="ACHUPT_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0818105.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34871..35278)
                     /locus_tag="ACHUPT_000364"
     CDS             complement(34871..35278)
                     /locus_tag="ACHUPT_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0818106.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35275..35838)
                     /locus_tag="ACHUPT_000365"
     CDS             complement(35275..35838)
                     /locus_tag="ACHUPT_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0818107.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35829..36299)
                     /locus_tag="ACHUPT_000366"
                     /pseudo
     CDS             complement(35829..36299)
                     /locus_tag="ACHUPT_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36483..37277)
                     /gene="thyA"
                     /locus_tag="ACHUPT_000367"
     CDS             complement(36483..37277)
                     /gene="thyA"
                     /locus_tag="ACHUPT_000367"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0818108.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37284..38159)
                     /gene="lgt"
                     /locus_tag="ACHUPT_000368"
     CDS             complement(37284..38159)
                     /gene="lgt"
                     /locus_tag="ACHUPT_000368"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0818109.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38310..40556)
                     /gene="ptsP"
                     /locus_tag="ACHUPT_000369"
     CDS             complement(38310..40556)
                     /gene="ptsP"
                     /locus_tag="ACHUPT_000369"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0818110.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40569..41099)
                     /gene="rppH"
                     /locus_tag="ACHUPT_000370"
     CDS             complement(40569..41099)
                     /gene="rppH"
                     /locus_tag="ACHUPT_000370"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0818111.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41452..41598)
                     /gene="ygdT"
                     /locus_tag="ACHUPT_000371"
     CDS             complement(41452..41598)
                     /gene="ygdT"
                     /locus_tag="ACHUPT_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0818112.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41784..42473
                     /gene="mutH"
                     /locus_tag="ACHUPT_000372"
     CDS             41784..42473
                     /gene="mutH"
                     /locus_tag="ACHUPT_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0818113.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42542..43255
                     /locus_tag="ACHUPT_000373"
     CDS             42542..43255
                     /locus_tag="ACHUPT_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0818114.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43393..43611
                     /gene="ygdR"
                     /locus_tag="ACHUPT_000374"
     CDS             43393..43611
                     /gene="ygdR"
                     /locus_tag="ACHUPT_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0818115.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43719..44759
                     /locus_tag="ACHUPT_000375"
     CDS             43719..44759
                     /locus_tag="ACHUPT_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0818116.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44792..45982)
                     /gene="lplT"
                     /locus_tag="ACHUPT_000376"
     CDS             complement(44792..45982)
                     /gene="lplT"
                     /locus_tag="ACHUPT_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0818117.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45975..48134)
                     /gene="aas"
                     /locus_tag="ACHUPT_000377"
     CDS             complement(45975..48134)
                     /gene="aas"
                     /locus_tag="ACHUPT_000377"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0818118.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48720..49751
                     /gene="galR"
                     /locus_tag="ACHUPT_000378"
     CDS             48720..49751
                     /gene="galR"
                     /locus_tag="ACHUPT_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0818119.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49758..51020)
                     /gene="lysA"
                     /locus_tag="ACHUPT_000379"
     CDS             complement(49758..51020)
                     /gene="lysA"
                     /locus_tag="ACHUPT_000379"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0818120.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51142..52077
                     /gene="lysR"
                     /locus_tag="ACHUPT_000380"
     CDS             51142..52077
                     /gene="lysR"
                     /locus_tag="ACHUPT_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0818121.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52064..52756)
                     /gene="ygeA"
                     /locus_tag="ACHUPT_000381"
     CDS             complement(52064..52756)
                     /gene="ygeA"
                     /locus_tag="ACHUPT_000381"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR0818122.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52885..54303)
                     /gene="araE"
                     /locus_tag="ACHUPT_000382"
     CDS             complement(52885..54303)
                     /gene="araE"
                     /locus_tag="ACHUPT_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0818123.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54618..55379)
                     /gene="kduD"
                     /locus_tag="ACHUPT_000383"
     CDS             complement(54618..55379)
                     /gene="kduD"
                     /locus_tag="ACHUPT_000383"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0818124.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55409..56245)
                     /gene="kduI"
                     /locus_tag="ACHUPT_000384"
     CDS             complement(55409..56245)
                     /gene="kduI"
                     /locus_tag="ACHUPT_000384"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0818125.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56532..57713)
                     /locus_tag="ACHUPT_000385"
     CDS             complement(56532..57713)
                     /locus_tag="ACHUPT_000385"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0818126.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57968..59197
                     /locus_tag="ACHUPT_000386"
                     /pseudo
     CDS             57968..59197
                     /locus_tag="ACHUPT_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            59624..>59763
                     /locus_tag="ACHUPT_000387"
     CDS             59624..>59763
                     /locus_tag="ACHUPT_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0818127.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT        14753 a        16036 c        14549 g        14425 t
ORIGIN      
        1 ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac ttactcatca actcatttca
       61 tttgttatat gaatgtttct taccaccctc acgcgacaaa tatcatcaca gttaatatgt
      121 catacaattt atgttgcaac acaaacgttt ccctattttc ataaaccgta ttttatcgct
      181 atgaaaaaga aattatcgcc atgattaact aaaagtattg attttttcag ttcaacctac
      241 atatattgcg cgccccggaa gaagtcagat gtcgtttaat gggcaaatat tgcccttaaa
      301 ttctctttta cttttgattt acagagtaaa gcgttgggat aatctatctt ccaagcagat
      361 tattgtattt gggatcaaga tcactgatag atacataact tgtgtgtatc tttccgccct
      421 caaattatta cggcggtaaa tgattaagcc atcgccgata gacagatttc atttttacgg
      481 tcaggcacct tcccgggctg aactggctaa aagctgaatt atttgcattc ctccaggaga
      541 aatagatgga aacgactcaa accagcacga ttgcgtcgaa agactctcgt agtgcctggc
      601 gcaagacaga caccatgtgg atgctgggcc tttacggcac ggcaatcggc gcgggcgtgc
      661 tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc gctgatcatc atggctatcc
      721 ttgcgttccc gatgacgttt tttgctcacc gcggcctgac tcgcttcgta ctgtctggta
      781 aaaacccggg cgaagacatc accgaggttg tagaagaaca ctttggtatt ggcgcaggta
      841 aactgattac cctgctctac ttcttcgcta tctacccgat cctgctggtt tatagcgtgg
      901 caatcaccaa taccgttgaa agcttcatgt ctcaccagct gggtatgacg ccaccgccgc
      961 gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac catcgttcgc tttggtgagc
     1021 agatgatcgt taaagcgatg agtattctgg tattcccgtt tgttggcgta ctgatgctgc
     1081 tggctctgta cctgatcccg cagtggaacg gcgcagcact ggaaacgctg tctctggaca
     1141 ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg gctggcaatt ccggtaatgg
     1201 tgttctcgtt caaccactct ccgatcatct cttctttcgc cgttgcgaag cgtgaagagt
     1261 acggcgatat ggcagaacag aaatgctcga agatcctggc attcgcacac atcatgatgg
     1321 tgctgaccgt aatgttcttc gtcttcagct gcgtactgag cctgactccg gcagacctgg
     1381 ctgcggctaa agagcagaac atctcgattc tgtcttacct ggctaaccac tttaacgcac
     1441 cggttatcgc gtggatggct ccgattatcg cgattatcgc tatcaccaaa tccttcctcg
     1501 gccactacct gggcgcacgt gaaggcttca acggtatggt gattaaatct ctgcgtggta
     1561 aaggtaagtc tatcgaaatc aacaagctga accgtatcac tgcgctgttc atgctggtaa
     1621 cgacctggat tgttgccacc ctgaacccga gcatcctggg tatgattgaa accctgggcg
     1681 gcccaatcat cgcgatgatc ctgttcctga tgccgatgta cgcaattcag aaagtaccgg
     1741 caatgcgtaa gtacagcggt cacatcagca acgtattcgt tgtcgtgatg ggtctgattg
     1801 caatctccgc aatcttctac tctctgttca gctaagtcct ttcgcgccgc tttcgggcgg
     1861 cgcttcctcc gttttaacgc gatgtatttc ctatgattag cgtattcgat attttcaaaa
     1921 tcggcattgg cccttccagt tctcataccg ttggaccaat gaaagcgggt aaacaattta
     1981 ccgacgatct gattgcccgt aacctgctta aagacgtgac ccgcgtggtg gttgacgtgt
     2041 acggctcgct ctctctgact ggtaaaggcc accacacaga tatcgccatt attatgggcc
     2101 tggcgggtaa cctgccggat accgtggata tcgattccat ccccggtttt attcaggatg
     2161 tgaatactca tggtcgcctg atgctggcaa acggtcagca tgaagtggag ttcccggttg
     2221 atcagtgcat gaacttccat gcagacaacc tttctctgca tgaaaacggt atgcgcatta
     2281 ccgcgctggc gggcgataaa gtcgtttaca gccagactta ctactctatt ggcggtggct
     2341 ttatcgttga tgaagagcat tttggccagc aggatagcgc accggttgaa gttccttatc
     2401 cgtacagttc agcagccgat ctgcaaaaac attgtcagga aaccgggctg tcactctctg
     2461 gcctgatgat gaaaaacgag ctggcgctgc acagtaaaga agagctggaa cagcacctgg
     2521 cgaacgtctg ggaagtcatg cgtggcggta ttgagcgcgg tatttccacc gaaggcgtgt
     2581 tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact acgccggatg ctggtcagcc
     2641 aggataaaac caccactgac ccgatggcgg ttgttgactg gatcaacatg tttgcactgg
     2701 cagtgaacga agagaacgct gctggcggtc gcgtggtgac tgcgccgact aacggtgcgt
     2761 gcgggattat cccggctgtg ctggcgtact acgataagtt tatccgcgaa gtgaacgcta
     2821 actcactggc tcgttacctg ctggtagcca gcgccattgg ttctctttat aagatgaacg
     2881 cgtcgatttc tggtgccgaa gtgggttgcc agggtgaagt tggcgtggcg tgctctatgg
     2941 cggcagctgg tctggcagaa ctgttaggcg caagcccggc gcaggtttgc atcgcagcgg
     3001 aaatcgcgat ggagcacaac ctcggtctga cttgtgaccc ggtcgcgggg caggttcagg
     3061 taccgtgcat tgagcgtaac gccattgcag cagtgaaagc ggtgaacgcc gcgcgtatgg
     3121 cactgcgccg taccagcgaa ccgcgcgtct gcctcgataa agttatcgaa accatgtacg
     3181 aaacaggtaa agacatgaac gccaagtacc gcgaaacctc tcgcggcggc ctggcgatga
     3241 agatcgttgc ctgcgattaa tctctcccag aggcctcgtt ttgcgaagcc tcttcctgtt
     3301 ttctcaacca gccatagcct gttccggcat cgaatgttac ccttatcgcc tgatctttaa
     3361 gggggttatc gtggctgttc atttgcttat tgtcgatgca ctgaatctta tccgtcgcat
     3421 tcatgccgtt cagggttcgc cctgtgtcga aacctgccag catgcgctcg atcagctcat
     3481 tatgcacagc cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc gcagtagcgg
     3541 ctggcgtcat cagcgtttac cagattacaa agcggatcga ccgccaatgc cggaagagtt
     3601 gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc
     3661 agccagcggc aacgaagccg atgacttagc cgccacactg gcggtcaaag tgacacaggc
     3721 cgggcatcag gcaacgattg tttcgacaga taaaggctac tgtcagttac tttcaccgac
     3781 attacgtatt cgtgattact tccagaaacg ttggctggat gcgccattta tcgataaaga
     3841 atttggcgtt caaccgcagc agttgcccga ttactgggga cttgcgggga tcagcagttc
     3901 aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc acgcagctgc tggtcgagtt
     3961 tcagagtctg gaagggatat atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa
     4021 attagaaacc cataaagaga tggcgtttct gtgccgcgat attgcccgct tacaaaccga
     4081 tttgcatatc gacggcaatt tacagcaatt gcggttggta cggtaacggc gagccggata
     4141 cgccgcaaat cgtatccggc attatcacat cagcgcattt accaggcggt atggtaaagc
     4201 tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac atcatccagt
     4261 gccgcctgag ccagtgccga aatgtcttcc ttgcgtacac caacatcacg caaatgtggc
     4321 ggaataccga catcacggtt gagggcaaac accgcttcaa cagcggcatt acgcgcccct
     4381 tccaggctca taccttccac tttcacgccc ataacgcgcg cgatatcgcg gtacttctca
     4441 ccggtaaagt cagcgttata gcgcatgaca tgcggtaaca ggatggcgtt cgcaacaccg
     4501 tgtggagtgt tataaaacgc gcccagtgga tgcgccatac catgcaccaa ccctaaccca
     4561 acattcgaga agcccatacc cgcaacatac tgcccgagcg ccatttcttc tccggcatcc
     4621 ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga tttcaatcgc tttaatgtgc
     4681 agtgcatcgg ttagtgccca cgcgccacgg gtaatatacc cctcaatagc atgagtgagc
     4741 gcatcaacac ccgtcgcggc tttcagcgcc ggaggcatac catccatcat gtcagcatca
     4801 ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa caaacttgcg ccgtttttct
     4861 tcgtcagtga tcacgtagtt aatggtcact tctgccgcag tacctgctgt ggtggggatt
     4921 gccagaatcg gtacactggg tttattggtc ggggaaagcc cttccaggct acgcacatcg
     4981 gcaaactccg ggttgttgct gataatgcca attgctttac aagtatcctg tggagaacca
     5041 ccaccaatag cgatcaggta atccgcgccg ctattctgga atacaccgag cccttctttg
     5101 acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa tcgcccatgc cagccctgca
     5161 gcatccatct tatcggtcac tttcgccacc acgccgcatt gcaccagcgt tttatcggtg
     5221 acgatcagcg ccttctgata accacggcgt ttcacctcat cggttaaagc cccaacagca
     5281 ccccgaccaa accatgccgt ttcgttcaga atcattctgt tagccatcat ccttctcctt
     5341 gttgctttac gaaattactc ttcaatccgt aacccatagg ttttgaattt ctccagcact
     5401 acggcaatct cttcatcgct cagcactggc accgggtccg taatcgccag ggtcgtcagg
     5461 taaagttgcg ccagcacttc aacttcatgc gccagccata acgctttttc cagattcacc
     5521 tcacaagcga taagcccatg atgttgtaac aaagttgcct tacgattttt gagagccagc
     5581 gcaacatgtt cagaaagttc gcgtgttcca aaggtcgcat aaggcgcgca aggaatagaa
     5641 ttaccgccag ccgccgcaat catgtagtga atagcgggga tcgatcggtt aagaatggaa
     5701 actgccgtgc aatgaacggc atgattgtga acaaccgcgt tggcatccgg tctgctttga
     5761 taggctgcca tatggaaacg ccattcgctt gaggggagct ttccttcctc atgtttaccg
     5821 ttgccatcaa taaagacaat atgcgactcc gtcagttttt catatggaat gcctgtaggc
     5881 gtaatcagca tcccatcctg ataacgtaca ctgacgttcc ccgctgtccc ctggttcagt
     5941 cccaggcggg tcatttccag gcaagtgtca ataatctgac gagcaagttt atttcgttcc
     6001 attagctacc tctctctgat tcaaaacagg gcaataatgt tgttcctttc acactattga
     6061 attagccgtt taattaccca ccatcttctt cctgattaac aagaaagaaa ttcacaagct
     6121 tatattttgt gacctagctc aactaatcac agtaaataac tgcaagttct ctttttataa
     6181 ccccgttaaa aatgaccgct cttaaaaata tttatcaaaa cggtcatttt tccatttccc
     6241 ctcgccagaa atgcccgttt tcggcacaaa caattaatac ggtcatctgg tttgtgtttt
     6301 ttatgattta ttttctgaaa cgggcatgaa atttcgatta ttaaagtgat ggtagtcaca
     6361 taaagtcacc ttccagctaa taagtgtgac cgccgtcata ttacagagcg ttttttattt
     6421 gaaaatgaat ccatgagttc atttcagaca ggcaaatatt caccgatatg aagcccgaac
     6481 tcgctggttt tgcacttttg aaaacataac cgattacgtg cttaagcttc tgaacctaag
     6541 aggatgctat gggaaacaca tcaatacaaa cgcagagtta ccgtgcggta gataaagatg
     6601 cagggcaaag cagaagttac attattccat tcgcgctgct gtgctcactg ttttttcttt
     6661 gggcggtagc caataacctt aacgacattt tattacctca attccagcag gcttttacgc
     6721 tgacaaattt ccaggctggc ctgatccaat cggcctttta ctttggttat ttcattatcc
     6781 caatccctgc tgggatattg atgaaaaaac tcagttataa agcagggatt attaccggat
     6841 tatttttata tgccttgggc gctgcattat tctggcccgc tgcagaaata atgaactaca
     6901 ccttattttt agttggccta tttattattg cagccggatt aggttgtctg gaaactgccg
     6961 caaccccttt tgttacggta ttagggccgg aaagcagtag tcacttccgc ttaaatcttg
     7021 cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt ctttgggcaa agtcttattt
     7081 tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa aatgtctcca gagcaattga
     7141 gtgcgtataa acacagcctg gtattatcgg tacagacacc ttatatgatc atcgtggcta
     7201 tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt cccggcattg cagagtgata
     7261 atcacagtga cgccaaacaa ggatcgttct ccgcatcgct ttctcgtctg gcgcgtattc
     7321 gtcactggcg ctgggcggta ttagcgcaat tctgctatgt cggcgcacaa acggcctgct
     7381 ggagctattt gattcgctac gctgtagaag aaattctagg tatgactgca ggctttgccg
     7441 ctaactattt aaccggaacc atggtgtgct tctttattgg tcgtttcacc ggtacctggc
     7501 tcatcagtcg cttcgcacca cacaaagtcc tggccgccta cgcattaatc gctatggcac
     7561 tgtgcctgat ctcagccttc gctggcggtc atgtgggctt aatagccctg actttatgca
     7621 gcgcctttat gtcgattcag tacccaacaa tcttctcgct gggcattaag aatctcggcc
     7681 aggacaccaa atacggttcg tccttcatcg ttatgaccat tattggcggc ggtattgtca
     7741 ctccggtcat gggttttgtc agtgacgcgg cgggcaacat ccccactgct gaactgatcc
     7801 ccgcactctg cttcgcggtc atctttatct ttgcccgttt ccgttctcaa acggcaacta
     7861 actgaacata ttttccgaat aaagtgagga atctgtaatg aaaaaaatca gcttaccgaa
     7921 aattggtatc cgcccggtta ttgacggtcg tcgcatgggt gttcgtgagt cgcttgaaga
     7981 acaaacaatg aatatggcga aagctacggc cgcactgctg accgagaaac tgcgccatgc
     8041 ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt atcgcgggta tggctgaagc
     8101 cgctgcttgc gaagaaaaat tcagcagcca gaatgtaggc ctcaccatta cggtaacgcc
     8161 ttgctggtgc tatggcagtg aaaccatcga catggatcca acccgcccga aagccatttg
     8221 gggctttaac ggcactgaac gccccggcgc tgtttacctg gctgcggctc tggcagctca
     8281 cagccagaaa ggcatcccgg cattctccat ttacggtcat gacgttcagg atgccgatga
     8341 cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc tttgcccgcg ccggtttggc
     8401 cgtcgccagc atgaaaggta aaagctatct gtcgctgggc ggcgtttcga tgggtatcgc
     8461 cggttctatt gttgatcaca acttctttga atcctggctg ggaatgaaag tccaggcggt
     8521 ggatatgacc gaactgcgtc gccgtatcga tcagaagatt tacgacgaag ccgaactgga
     8581 aatggcactg gcctgggctg ataaaaactt ccgctatggc gaagatgaaa ataacaaaca
     8641 gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc gaaagtttac tgatggcgat
     8701 gtgtatccgc gacatgatgc aaggcaacag caaactggcc gatattggtc gcgtggaaga
     8761 atcacttggc tacaacgcca tcgctgcggg cttccagggg caacgtcact ggaccgatca
     8821 atatcccaat ggtgacaccg ccgaagcgat cctcaacagt tcatttgact ggaatggcgt
     8881 gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt aacggcgtgg caatgctaat
     8941 gggtcaccag ctcaccggca ccgctcaggt atttgccgat gtgcgtacct actggtcacc
     9001 agaagcaatt gagcgtgtaa cggggcataa actggacgga ctggcagaac acggcatcat
     9061 ccatttgatc aactcaggtt ctgctgcgct ggacggttcc tgtaaacaac gcgacagcga
     9121 aggtaacccg acgatgaagc cgcactggga aatctctcag caagaggctg acgcttgcct
     9181 cgccgctacc gaatggtgcc cggcgatcca cgaatacttc cgtggcggcg gttactcttc
     9241 ccgcttcctt accgaaggcg gcgtcccgtt caccatgact cgtgtcaaca tcatcaaagg
     9301 cctgggaccg gtactgcaaa tcgcggaagg ctggagcgtg gaattgccga aggatgtgca
     9361 tgacatcctc aacaaacgca ccaactcaac ctggccaacc acctggtttg caccgcgcct
     9421 cactggtaaa gggccgttta cggatgtgta ttcggtaatg gcgaactggg gtgccaacca
     9481 cggagttctg accatcggcc acgttggcgc agactttatc acccttgcct ccatgctgcg
     9541 tatcccggta tgtatgcaca acgttgaaga gaccaaagtg tatcgtcctt ctgcctgggc
     9601 tgcgcacggc atggatattg aaggccagga ttaccgcgct tgccagaact acggtccgtt
     9661 gtacaagcgt taatacatta caccctgatg tgatgcccgg tcgctccggc taccgggcct
     9721 gaacaagcaa gagtggttag ccggataaac aatgttatcc ggctatattg caggagcgat
     9781 tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg accaatgtca gggccatcgc
     9841 ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg cctaatgcca gcgatatcgc
     9901 gatggaaaac aacacctggc accagtggtc tttagactgc cattttgcaa cgctttgctg
     9961 attgctgtcg gcaaatcaat agtgaactga ctgaatgcca catccgcggt atcgccgtca
    10021 ccacctttag tgtggatggc gctctggtag ataagcaagg caatctgctc tatccgatca
    10081 ttagctggaa atgtccgcga acagcagcgg ttatggacaa tattgaacgg ttaatctccg
    10141 cacagcggtt gcaggctatt tctggcgtcg gagcctttag tttcaatacg ttatataagt
    10201 tggtgtggtt gaaagaaaat catccacaac tgctggaacg cgcgcacgcc tggctcttca
    10261 tttcgtcgct gattaaccac cgtttaacca gcgaattcac tactgatatc acgatggccg
    10321 gaaccagcca gatgctggat atccagcaac gcgatttcag tccgcaaatt ttacaagcca
    10381 ccggtattcc acgccgactc ttccctcgtc tggtggaagc gggtgaacag attggtacgc
    10441 tacagaacaa cgccgcagca atgctcggct tacccgttgg cataccggtg atttccgcag
    10501 gtcacgatac ccagttcgcc ctttttggcg ctggtgccga acaaaatgaa cccgtgctct
    10561 cctccggtac atgggaaatt ttaatggttc gcagcgccta ggttgatact tcgctgttaa
    10621 gtcagtacgc cggttccacc tgcgaactag atagccaggc atggttgtat aacccaggta
    10681 tgcaatggct ggcatccggc gtgctggaat gggtgagaaa actgttctgg acggctgaaa
    10741 caccctggca aatgttgatt gaagaagctc gtctgatcgc acctggcgcg gatggtgtga
    10801 aaatgcagtg tgatttattg tcgtgtcaga acgctggctg gcaaggagtg acgcttaata
    10861 ccacgcgggg gcatttctat cgcgcggcgc tggaagggtt aactgcgcaa ttacagcgca
    10921 atctacagat gctggaaaaa atcgggcact ttaaagccta tgaattattg ttagtcggtg
    10981 gaggaagtcg caacacattg tggaatcaga ttaaagccaa tatgcttgat attccggtaa
    11041 aagttctcga cgatgccgaa acgaccgtcg caggagctgc gctgttcggt tggtatggcg
    11101 taggggaatt taacagcccg gaagaagccc gtgcacagat tcattatcag taccgttatt
    11161 tctacccgca aactgaacct gaatttatag aggaagtgtg aaatgctgaa aacaatttcg
    11221 ccgttaattt ctcccgaact attgaaagtg ctggcagaga tgggacatgg agatgaaatt
    11281 attttttccg atgctcactt tcccgcccat tcgatggaac cgcaggtgat ccgcgctgat
    11341 ggcctgttgg tgagcgactt gctccaggcg attatcccgt tatttgagct ggacagttat
    11401 gccccgccgc tggtgatgat ggcggcggta gagggtgaca ctctcgatcc tgaagtagaa
    11461 cgacgttacc gtaatgcgct ttcactacaa gccccgtgtc ctgacatcat ccgcatcaat
    11521 cgttttgcgt tttatgaacg ggcgcaaaaa gcctttgcga tcgttatcac aggcgaacga
    11581 gcgaagtacg ggaatattct tttaaaaaaa ggggtaacac cgtaatctca taccggtacg
    11641 cccgcatgac gcgggcggtt atcgaatgat ggggtgaaaa atatgaaagc ggcacgccag
    11701 caagcgatag tcgacctgct gctgaaccat accagcctga ccacggaagc tctctctgaa
    11761 cagctaaagg tcagtaaaga aaccattcgt cgcgatctca atgaattaca gacgcagggt
    11821 aaaattctgc gcaatcatgg acgcgctaaa tatatccacc gtcaaaatca agacagtggc
    11881 gatccctttc acatcaggct gaaaagccat tatgcgcata aagcagatat cgcgcgcgag
    11941 gcgctcgcgt ggattgaaga agggatggtg atagccttag acgccagttc aacttgctgg
    12001 tatctggcac gccagttgcc tgacatcaac attcaggtct tcaccaatag ccatccgatt
    12061 tgccatgaac tcggtaaacg cgaacgcatt caactgatca gttccggcgg cacacttgag
    12121 cgcaaatatg gctgttacgt caatccctcg ctgatttccc aacttaaatc gctggaaatc
    12181 gatctgttta ttttttcttg tgaagggatc gatagcagcg gcgcactgtg ggactccaat
    12241 gcgatcaacg ctgattacaa atcgatgcta ttaaaacgtg ccgcgcaatc gttgttattg
    12301 attgataaaa gtaaatttaa tcgttcaggg gaagcccgca tcgggcatct ggatgaggta
    12361 acgcacatta tttctgatga gcgccaggtt gcaacttctt tggtaacagc ctgacggaaa
    12421 gggtagcagg ccggagacga cgccccggcc ttgcctgtta tcgctcgtcg cgacgaccac
    12481 ccaccgcagc ccagatgcgg cggacgtgca ccgtcacttc ttcgcgatcg tgatacaact
    12541 gccgcgcctg aatctgagca tttatgccat gttcatcaag ctgtgcctga atatacgcca
    12601 gattgtgtga tacttcttcg tagcgttttt tcatcggcag tttgagattg aaaatggttt
    12661 cacggcacca gccattaacc agccactgcg ccatcaatgc cgcaacttta gccggttttt
    12721 caaccatatc gcataccatc caggagatat tgctgcgtgt cggacggaat ttgaaaccgt
    12781 cttcccgcag ccacgtcacc tgcccggtat ccatcagact ttgcgccatc gggccgttgt
    12841 cgacggaata aacccacatg ttgcgcttca ccagttggta ggtccagccg ccagggcaag
    12901 cgcctaaatc caccgcccac atcccgttcg ccaggcgttc atcccactca tccgcaggaa
    12961 taaacacatg aaatgcctct tccagtttga gcgtggaacg actcggcgcc tctgccggaa
    13021 atttcaggcg cggaatgccc atatagaacg gcgaattatt gttgctgtat gagtaaccgg
    13081 tatagcagca tcctggtgca ataaagaata catgcacaac cggacgcttc ggcgtttcat
    13141 agttcgccag caccccagca tcgcgcaagg cagcgcgtag cggaacagtg aacttacggc
    13201 agaatttcag caactctttg ctttcgttgg tatcggcaac ttcaacacgc agttcaccgc
    13261 ccttctctac tacaccctgt aacatgccga caatgggggt aatacgatct tctggcggca
    13321 aatgctgcag gagttccccc accacaaacc actggcgggc aaaaattaat gaactgaacg
    13381 gcagctcacg gattaactta tcgccatcat caggttgata acattcataa atgacataac
    13441 ccgcattctc tttcacgcgg gcaaaaccga aaatttcccg ctggccggct ttatcggtaa
    13501 tttctgcggc gcactctttt tcaaagcccg gacggcacag caatacaacc ttattcatga
    13561 cttacgccct tgcgctttaa acggatagca ccaactaaca ttaacgccca gcccgcaagg
    13621 aagctcacgc cgccaaccgg tgtgacaaac gcccacaaac gcagatggga cagcgccagg
    13681 caataaaggc tgccgctgaa cagcaccgtg cctaacgcga ggaaaacgct actccagtaa
    13741 aaccagatac tgatgcgacg ctgcattgcc accgccagac ctaagatcgc cagcgtatga
    13801 aacgcctggt attcgagacc ggtttggatc cagcccatct caacggcccc catggtttta
    13861 cttaacacat gcgcaccaaa agcgcccagt gccacaaaaa tgaagccgct aatggcggcg
    13921 aaaatcagca taaaacggct ggtcatggtc gtaccctacg taaattattg ttcataacga
    13981 aagcggaatt tttcttgttc agcagcggct ttcgccagga tccattggcg aaaggcggct
    14041 attttaccca gttctgcctg gctgtcatga caaaccagat aaaaagcatt tttactgacc
    14101 agaacatcat taaacgggca aacaagacgt ccggcctcga tttcagattg cgccatcacg
    14161 ttatttgcca gcgccactcc ctgcccgtga atagccgctt gcagcaccat ggcgctatga
    14221 ctaaaaattg gcccttgctg aacgttgata tgattaaacc ccaactgtcg ggtatatgtc
    14281 tgccagtcac ggcgtgacgc atcatgtaat aacgtatgtt tagccagatc ttccggggtc
    14341 ttcaagggtt tttcgcctgt cagcagtagc ggcgaacaca ccggcaataa atattcggcg
    14401 tacagttttt ccacccgtag ccccggccag ttgccccgac cataaaatat cgccacatca
    14461 acatcatccg ccagcttatc ttcctgacga tcaaccgcct ggattcgaac gtcaattccc
    14521 ggataagctg aattaaagct ggaaagtcgc ggaaccaacc aatgaatggc gaaactgggg
    14581 agtaaactga ccgtcaacgc ccccttggcg ctacgggcct ggagtttacg cgtcgcttcg
    14641 gttaattgcg aaaatatctc tttgatatcg aggaaatagc tttgcccttc ctcggtcagc
    14701 aggagtgaac gattacggcg gcggaacagt tttagcccca aaaaatcctc aagagacttg
    14761 atttgatgac ttactgcggc ctgggtcaca aaaagctctt ctgctgcgcg agtaaaactt
    14821 aaatggcgtg ctgcggcatc aaaaactcgt aaggcattta gcggtggtaa tcgtttagac
    14881 atggctttta aactttgatg ttaaatgaat ttaacaatta gatcacactg tgtaacctat
    14941 tagttttttt aatctgagcc attataaatt gtccgttgag cttctaccag caaataccta
    15001 tagtggcggc acttcctgag ccggaacgaa aagttttatc ggaatgcgta ttctggtgaa
    15061 cttttggctt acggttgtga tgttgtgttg ttgtgtttgc aattggtctg cgattcagac
    15121 catggtagca aagctacctt ttttcacttc ctgtacattt accctgtctg tccatagtga
    15181 ttaatgtagc accgcctaat tgcggtgctt ttttttacct tgcgatcgcg aattactgtt
    15241 ccagttcgac catctctttc acgtcagtac ggttgatctg ctgtttgttg ccattagcgt
    15301 ctttatacga aatcataccg atatcgttat cagtctgtgg tttgccgtca gagacgatgg
    15361 tacgtccgtc attggtgtgc atcacatagt tcgaaccgga acaggcgctc agggcaaaag
    15421 tcagcataca ggcagaaata attgcggcag tctttttcat catcttctcc ttaaaacgat
    15481 taataaatct taatagtatt gattaacagg ctaaaattaa cgcctaacac tattcagcat
    15541 atgttactta cgcggcttcg ccaggatatc cagataattc tgatggttag cactctcctt
    15601 gtatcaaagt gaattttgcg tcacgatcgg tgcatcaagc cgaggagtac catgaacgtt
    15661 tttaatcccg cgcagtttcg cgcccagttt cccgcactac aggatgcggg cgtctatctc
    15721 gacagcgccg cgaccgcgct taaacctgaa gccgtggttg aagccactcg acagttttac
    15781 agcctgagcg ccggaaacgt ccatcgcagc cagtttgccg aagcccaacg cctgaccgcg
    15841 cgttatgaag ctgcacgaga gaaagtggcg caattactga atgcaccgga tgataaaact
    15901 atcgtctgga cgcgcggcac cactgaatcc atcaacatgg tggcacaatg ctatgcgcgt
    15961 ccgcgtctgc aaccgggcga tgagattatt gtcagcgtgg cagaacacca cgctaacctc
    16021 gtcccctggc tgatggtcgc ccaacaaact agagccaaag tggtgaaatt gccgcttaat
    16081 gcgcagcgac tgccggatgt cgatttgttg ccagagctga ttactccccg tagtcggatt
    16141 ctggcgttgg gtcagatgtc gaacgtgact ggcggttgcc cggatctggc gcgagcgatt
    16201 acctttgctc attcagccgg gatggtggtg atggttgatg gtgctcaggg gacggtgcat
    16261 ttccccgcgg atgttcagca actggatatc gatttctatg ctttctctgg tcacaaactg
    16321 tatggcccga cgggtatcgg cgtgctgtat ggcaaatcag aactgctgga agcgatgtcg
    16381 ccctggctgg gtggcggcaa aatgattcac gaagtgagtt ttgacggttt caccactcaa
    16441 tctgcgccgt ggaaactgga agcaggaacg ccaaatgtcg ctggcgtcat aggattaagc
    16501 gcggcgctgg aatggctggc agattacgat atcaaccagg ccgaaagctg gagccgtagc
    16561 ttagcaacgc ttgcagaaga agcgctggcg aaacgtccag gctttcgttc attccgctgc
    16621 caggattcca gcctgctggc ctttgatttt gctggcgttc atcacagcga tatggtgacg
    16681 ctactggcgg agtacggtat tgccttgcgg gccgggcaac attgcgctca gccgctactg
    16741 gcagaattag gcgtaaccgg cacactgcgc gcctcatttg cgccatataa tacaaagagt
    16801 gatgtggatg cgctggtgaa tgccgttgac cgcgcgctgg aattattggt ggattaatga
    16861 caaacccgca attcgccgga catccgttcg gcacaaccgt aaccgcagaa acgttacgca
    16921 ataccttcgc accgttgtcg caatgggaag ataaatatcg ccagttgatc atgctgggga
    16981 aacagcttcc ggctttgcca gacgagttaa aagcgcaggc taaagagatt gccggatgcg
    17041 aaaaccgcgt ctggctggga tataccgtgg ctgaaaacgg caaaatgcac ttctttggca
    17101 acagcgaagg gcgcattgtg cgcggcctgt tggcggtgtt gttgactgcc gttgagggga
    17161 aaaccgccgc cgaattacag gcacagtcgc cgctggcatt gtttgatgag ctgggattac
    17221 gtgcgcagct tagcgcctca cgcagccagg ggttaaatgc gttaagcgag gcgattatcg
    17281 ctgcgacgaa gcaggtttaa gcacagaacc gggtcggata agacgttcgc gtctcatccg
    17341 acctgattgt ttaaccctga cgcgccgctt tcgccatcat cttcttcagc gcatgagaaa
    17401 ccgcaacaaa cccaaaggtg gcggtcacca tcgttgccgc gccaaatcca gatgcacaat
    17461 ccatccgctt cggcccttct gccgtggctt tcatcgcaca caccgtaccg tctgactgcg
    17521 ggtacaccag cgcttcagta gaaaacacgc aatccacgcc gagcttacct ttactgtttt
    17581 tcactacgcc aaaatcgctt ttcaggcgct cgcgcaactt cgccgccagc ggatcctgaa
    17641 tcgttttcgc cagatcggta acctgaatct gcgtcgggtc aatctgccca cccgcgccac
    17701 cggtcgtgac cagcgggatt ttattgcgcc gacaataagc aatcagcgcc gctttgggcc
    17761 gtacactatc aatggcatca atcacgtacg aataaccggc actcatatat tgcgctacgt
    17821 tatccggcgt cacgaaatca tccaccaccg ttacacggca ctccgggtta atctggcgaa
    17881 tgcgctccgc catgacttcc gcttttgcca gcccgacgtt atcgcgcaga gcgtgaattt
    17941 gccgattggt attggtgacg cacacatcat ccatatcgat aagcgtgatt gcgccaatcc
    18001 ccgtgcgcgc cagcgcttcc gccgcccagg aaccgacacc accgatacca accacacaaa
    18061 tatgcgcgtc agcaaacagt tgcaacgctt tttcaccata cagacgcgct gtgccaccaa
    18121 aacgctgacg ccatgcatca ctaattacca cagacatagc acctcagaat taaaaagggc
    18181 gaagttatcc ctcaccctaa acgcataaat gcctgatgcg ctacgcttat caggcctaca
    18241 taactcctgc aatattttgg atttgcatga tttgtaggcc ggataaggcg ttcacgccgc
    18301 atccggtatg aacaacgcgc acttagtcaa taatcaggat gtgaggttac ccctcccctg
    18361 tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc
    18421 ccggagccgt tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc
    18481 cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga
    18541 catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca
    18601 gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa
    18661 cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg
    18721 gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt
    18781 gtgtttcgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac
    18841 ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga
    18901 aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa
    18961 tgaagttatc catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgccccgg
    19021 cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat
    19081 actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa
    19141 actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc
    19201 gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac
    19261 tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc tcggcaaaat
    19321 caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg
    19381 taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa
    19441 gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct
    19501 tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga
    19561 gcagcctctc cctgacgcaa attttgcaca aaaaacaggc tttagtggtt tgtttttgtt
    19621 caaaatcatg ctaaatccgt gatcggggta aaaaaaaggt tgcatgaaaa cgcgagcgga
    19681 gtatagtgcg catccacgga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac
    19741 ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatt attgaacacc ctaacgggtg
    19801 tttttttgtt tctgatctcc cataaaaaaa gcgccattca gcgccttttt atcatcccct
    19861 tctcgccagc gttgccccat cggcaaaata cgctttaatc cccgcaagaa tagactccgc
    19921 aacttcctgc tggaaagtcg ccgttttcag tttacgctct tcctcaacgt tactgataaa
    19981 cgccgtttcg accagaatgg agggaatatc tggtgccttt agtacggcaa acccggcctg
    20041 ttcaacttga tttttatgca gcttgttgat ttttccgagc ttattcagta ccgctttacc
    20101 aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata tcgaacatgg tgtggtcgac
    20161 atagcggtca ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc
    20221 cagatatttt gccgcagtac tggttgcgcc tttggttgag agcgcaaaca ccgaggaacc
    20281 gctcggctga cgactggtaa aggcgtcggc atggatagag acaaacaggt cagcacgctg
    20341 tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat
    20401 gtacaccttc atattgccct cttcctcgat cagagagcgc aaacggcgag ctatttgcaa
    20461 taccacatct ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt cgccaccgtg
    20521 gccagggtca agcataatga caatcggacg atcgcgccct gctttacccg gttgtggacc
    20581 actttgcgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc
    20641 cagcagcggg tcctgcatat cctgtgcatt ggccggatag aggtccatca ccagacgctc
    20701 tttaaacccg gcgaccggcg caagggcaaa cagctgcggt tttacgtttt gctttaattc
    20761 aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg
    20821 gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc gagttcaggt ttacatcttc
    20881 gatatccacc accacgcgtt cagggttact caacgcgaac tgcttatatt tcagctgacg
    20941 atttgattct accgtcacgc gggtgtagct ggacgcaggc cagacgcgca ccgccacgac
    21001 ctggctaacc gcagccaggc tgacctgact tacgctcaat agccacatag cgcccgcgcc
    21061 ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc tcccgagcaa
    21121 aagaaatcta agctgtgtaa caagtaaacg actaatttga ccggaaactt tagcgaaaga
    21181 cgcataatct gtcatctaat aaacggtaaa cattcttttt atattcacgg cattactgat
    21241 aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat
    21301 acaataattt caaataatca tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag
    21361 ctggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg aaaaacgttt
    21421 gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat cgttaatgat
    21481 atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc acgtccgcag
    21541 atcgacgcaa atctggctgc gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg
    21601 accgacgcca aaacactgga actggtgaag caggctgcgg gaacattgca actggatatt
    21661 actgctcgcc tgtcaatgag tctcaataac acgccgctgc agggcgcgca tatcaacgtc
    21721 gttagtggca attttattat tgcccagccg ctgggcgtcg atgacggcgt ggattactgc
    21781 catagcgggc gtatccggcg gattgatgaa gacgcgctcc atcgtcaact ggaaagcggt
    21841 gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg gcgagagctt taatctgacc
    21901 tcggaagaga ttgccactca actagccatc aaactgaaag ctgaaaagat gattggtttt
    21961 tgctcttccc agggcgttac taatgacgac ggtgatattg tctccgaact tttccctaac
    22021 gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg attacaactc cggtacggtg
    22081 cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgccgctg tcatttaatc
    22141 agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg tatcggtacg
    22201 cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga tattggcggt
    22261 attctggagt tgattcgccc gctggagcag caaggtattc tggtgcgccg ttctcgcgag
    22321 cagctggaga tggaaatcga caaattcacc attattcagc gcgataacac gactattgcc
    22381 tgcgccgcgc tctatccgtt cccggaagag aagattgggg aaatggcctg tgtggcagtt
    22441 cacccggatt accgcagttc atcacggggc gaggttctgc tggaacgcat tgccgctcag
    22501 gcgaagcaga gcggcttaag caaattgttt gtgctgacca cgcgcagtat tcactggttc
    22561 caggaacgtg gatttacccc ggtggatatt gatttactgc ccgagagcaa aaagcagttg
    22621 tacaactacc agcgtaaatc caaagtgttg atggcggatt tagggtaagc gatgaaaatc
    22681 accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg caattacgtt tattcccgcg
    22741 aactaaacaa cgccgccaga ccactgcgcc gctcagtacg agtggcgatt gccgcactta
    22801 atatacgctc atcggcatac agcgacagac gacgacgcgc gcgggtcacc gcggtgtata
    22861 ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga
    22921 actccgatcc ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc
    22981 gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg cgcgtcccct
    23041 gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac
    23101 gggcaatcat caccggtcgg ccttcgtacc aacgagagtg cggatgacga tgaattttgc
    23161 gcttctgttg cataaactgt tcaattcgct cattcagtcc agccacgcca aacggccctt
    23221 cccgcagggc gcacaaaagc tggtactcat tgaacgcctg aatgattaaa tccggctcgg
    23281 cacgcgcttg cagcagatcc agataacgtc cgtaacccgc aagagcttcc tcaagcatcg
    23341 caatataatc ttcgccgctc tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa
    23401 aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc
    23461 cagaatcgct gccgaaacga tagctttttt gcagcaggca gagactgtcg cgcaaagatg
    23521 ccgcttctgt gccggttcct gccggaacgt gagtccccgt caggcggctt agctgcctgg
    23581 cacgctcggc ggtaaagccc gcgttggcat aagcgcagat atcgcccagc acagccccag
    23641 cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca tgatctggca
    23701 aggcgtcgat cagtctcgac atcataggca gatcgatcat tgacgcttca tctaccacca
    23761 gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta cccggctgcg
    23821 cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt tgttcatcgg
    23881 tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg gcagctttac
    23941 ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga attaacgctg
    24001 ccagcaactt cgccacggtg gtcgttttac cggtgcctgg gccgccggaa atcaccgaga
    24061 tccgccgcgt cagcgccact gccgctgcaa ctttttgcca gttaatttca tcgcttactg
    24121 gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg gtatgattca
    24181 cctcattgaa gaagcgcgct accgtccgct cgttacacca catgcgattc aaataaagac
    24241 gatcgccaca gaggatcatc ggcgtgggtt catctcccct gctgaccgct tgagaagcca
    24301 gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc gccaacagcg
    24361 gattcgacgc ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg
    24421 catcatgact taacagtgcc gccgcgaggg tgacggcagg atgttcatct cccgccacgg
    24481 tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg ctccacagct tccagtaatt
    24541 gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct catccatcag
    24601 ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat gttctttatc
    24661 aacgccacgc aggaacagat agataacgcc gccaaagtga agatcatagt cgtaatcagc
    24721 aatgcgatgg cgcagataac gatgcagcgc cagggtataa agctgatatt gcagatcata
    24781 gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg taagccgaac tgtcttcacc
    24841 cagccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga ataccaggtc
    24901 gataaagcct tttaacatgc cgcgtacctg catgaactcc agcggcgggc agccagcgga
    24961 tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg gttcgctaat
    25021 cggcaggtaa aactccatct ccacctgttt atcgcggtcg gaaagctgac tcaggctaac
    25081 acccgtttca ttgagaggtg cctggaggac agccgtgatc cactcggtca aaaccggttc
    25141 ccactgcgat tcaaagccgc cgagttccag tttttcctgt acccagttcg ggtcaaccgg
    25201 ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg acgcaccgcg
    25261 cgggaactga tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc
    25321 aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct gcaagccgga
    25381 gtagctggtg acacgccagt tatcgccggg caatcgttgt agtgtcctcg cattcagttc
    25441 tgcagttaaa gcatcattaa cctgccaggg ctggttatca ccagtttgtg ccgtttgcca
    25501 ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg catcttgcgg
    25561 ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt cacctttttt
    25621 atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt
    25681 cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcgacctccg cgaggtcgac
    25741 gctttctggc gcagcattaa gatccaaaac tgcctcaaac gagtggcgat cgtgataaaa
    25801 cgcctgatcc tggacgcgga aattggtaat aaatggcaac cagaccagcg gatattccag
    25861 ccctttcgat ttatggatcg tgacaatctg caccagatgt ttatcacttt cgaggcgcat
    25921 ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc agcgtaccag
    25981 cgcatgttca ctttccagct gcgttccggc ttcttgaagc agttcgctga tatgcaagat
    26041 atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc
    26101 cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt gccgataacc
    26161 atcgaactct tcgactaccg catcccacgc atgttcgtca ttattcagcg tttcaatatc
    26221 cagcgcattc agccccatca ttgacgttgc cagcgcactg cgcagggtgt tctcacgttc
    26281 gggcgtcatc accgcctgca acaaccaaag catttcctgc gcttccagag tttcaaaaac
    26341 actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg catcgcgcac
    26401 ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg tccgaagcac gcaccggacg
    26461 cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg
    26521 gatttgcgca gcacatacct gcgccatggt actttgatag tcgccaacgc cgcagctttc
    26581 gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaata caaaacgtaa
    26641 cgcctgattt ttcccggctg atttcactgg aataaacggt atttcgcgaa acatgaacgt
    26701 gtcatcagtc tggctgaaaa gcttgttcac actgttcacc attccaggtg cggagcgcca
    26761 gttggtgtct aaagtgtagt gggcatgaac ttcactacgc gccttcatat aagtgaagat
    26821 atccgcaccc cggaatgcat atatggcctg cttcgggtcg ccaatcagca acaatgcggt
    26881 ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt cggtatcctg
    26941 aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca acacctcgcc
    27001 gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac ccaattcgcc
    27061 acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc gggtgatcaa
    27121 cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa acagtggatg
    27181 tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag aaacgctggg agaatttttc
    27241 cagcgactcc ggcaactgat aactgtttgt ctcttcttct gcccaggcgc tgatcttttc
    27301 gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag aagattcgat
    27361 cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc
    27421 cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg ctttgataac
    27481 cggcggttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg gccctttcca
    27541 ggtttcaaag acgacctggg ctatttcacg cggcagcggg tagcagtggc gacgccagaa
    27601 atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct gctcaaacag
    27661 catgccggat tcaaaggcat tcaggttgag catgcgctgg caaaagccgt gaatagtaaa
    27721 gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg cttgcgcttt
    27781 atcgtcgatc tcttccagca ggcgttcgta cagtggattg tcggtggttt cacgcagaca
    27841 ggcgatgcgc aactcgtgga tattgctacg gatacgaccg cgcaattctg ccgtggcagc
    27901 ctcggtaaag gtgaccacca gcagttcttc aacgatcagc gggcggggaa aggcggcgga
    27961 accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt
    28021 gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt
    28081 ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc aacgcgctga
    28141 cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg ttctggctgc
    28201 cggaaatctg cgacagaata gccatgcctt gcggctcgac caccgcctga tggaagaaat
    28261 cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca cgcgaatcga
    28321 agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg ccgagcgttt
    28381 gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac tcatctggct
    28441 tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga
    28501 atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga cgccccacgc
    28561 tcattggaaa cgcaaacacg gcatagccca attgttcttc ggtacgcaac tgattgtaga
    28621 accacggctg tacgatctgc cccaacagag agctataggc tgagctggtg tattcatcgt
    28681 agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttaccg gctttttcaa
    28741 agatgacgga ttgtttttta tcgaccacta catctttgtt tcgacaccac tctgaaccat
    28801 cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc tcggtcatgt
    28861 tgccgataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga taggccagca
    28921 cctctttcaa cgtaatggag ggcaaaattt tacgccgttc atctcgcgag aagtacggca
    28981 cttgcgagag catctgcgcg ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg
    29041 aatccatcat ctggttatac caggacttcg cctgctcaag ctgctcttcc gtagcggtat
    29101 agctaaagta cccctcgagc aatgcctgga acagctgcgg cagacgctgg gtgtaaccat
    29161 tagcattaat cataaggccg ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg
    29221 cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc gcaaacatca
    29281 cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg acatcagctt
    29341 tgggctcgct ggcaaaataa cggcttggcg catacaccac gcgcagattc gactcatcaa
    29401 caatcagctc tggatggtcg tatttcttct ctgacttaat cagcgagaaa tcatcaggaa
    29461 tataagggtt aagctctggc aaagagagcg caatgtcggc ggcttttttc tgccagtcgg
    29521 cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc atcgacaaag taagccgttt
    29581 tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc gtcatcatcg
    29641 ccagacgttc ctttactgct ttagcatcgt accgatcggc aatattgact gcatccagcg
    29701 tatgctcaac aggaacgcga atcatggtat ctgccagcca ttcgacgtaa tccatatcac
    29761 gggtgatcga cggataacgg aagtcgatat ccagcacatt cgccagttca tcgaagtatt
    29821 gtttatcaat gcctttttca cgtaacagat tgagatagct aaaaattgcc gccacaacct
    29881 gatcgcgatt agccaggcct ttatcagtta aagacgcaga gatcgctaat acgccgctgt
    29941 tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc tgcttttgca
    30001 gccagtcaga aagtgtacct gggctgcgat tgccaatcag ataggtaatc aattcatcgg
    30061 ttttactacg gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc aacactttac
    30121 gcggcagcgc agggacgtaa tgaatgataa tgcccttttg cgcgtcggtg actaccggca
    30181 cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg tccgccgcca
    30241 tttttgccaa ctccggcagc ggtttattac tgtaaataac cgccttcatc aaattggcgg
    30301 aatagtactt ctcgtggaaa tctttcagcg cctgctgcac cggattacca ggtttgtcgc
    30361 ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt
    30421 ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt
    30481 tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag caagggttct gcaatagcat
    30541 cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc aacttccaga tagaaagccg
    30601 tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga tattcggcca
    30661 gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca aggtaatgtg
    30721 ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcactacc agcgccgaga
    30781 gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta tccagacgta
    30841 tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga atcggctgcc
    30901 atcccgtttc tgcctgactt aatggtgccc agagggcaac aaacaacaat aatgctttga
    30961 accaggtgct gcggggcatt cacggacctc ataatcaact ttattttctg gacagattca
    31021 acaggttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt cagcctatgt
    31081 ttatataacc atcagtccgt gactggtgcg catcataaag taagcagata gattgcgcaa
    31141 tttttataca acactcatga ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac
    31201 tgttcaacga tggcctccat tgtctctggt gttaattgcc gccagagccg ttgataccag
    31261 atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag gaatttcgta
    31321 cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc gtcataacag
    31381 gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga cattccttca
    31441 cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga
    31501 aaacgccact caccgtcttt gcgtagaaaa aggcgacttt cgccattacc accgctggca
    31561 cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa taaagaggga
    31621 cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat ctgcacaccg
    31681 ttgcaggcga gatcaatttc catactctgc cccggctggc gacaggcaat gactctgtcg
    31741 gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac
    31801 ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc
    31861 agtgcattca ataactgctg attgatttga taacggctaa gtccttccag aataaatggc
    31921 tcggtgtctg ggatttcgct gtcttcagta tggaagttca cctgcaaacg catctggaag
    31981 aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt
    32041 aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg gctggccgca
    32101 ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg cggatcaaac
    32161 ggcatccggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct ttcatcacag
    32221 ttgagcgctt catcgcccgg tagataatga ctttgcccga tgtagtcgat cagttcctgc
    32281 accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat atagctgata
    32341 tagagttttt gctgcgcgga aattaacgct tccaggaaca ggtagcggtc gtcatcgcga
    32401 cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc
    32461 tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa cggaattgaa
    32521 cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg gctgatacgt
    32581 tcttgatcca gacgctgtgc cagttcatca cgcaatagtg acagcggcac cgcgtcgcca
    32641 tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat cagcgtcatc
    32701 gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg acaaaccggc
    32761 aaccactctt ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt
    32821 agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc atcataaggt
    32881 agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc
    32941 aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg
    33001 tcatctatcc cccaacgaat gccggattcg ttgacccact ggcgtaaata acgcagccct
    33061 tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagcagcgc cagcacatcc
    33121 tctgacacaa agcgactgtc aggcagtgat aacaggctga taaacgcttc cagtaccgga
    33181 tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg
    33241 ctaccaaaca ctgcctgaat aaacggactg tagctgtcga tatcagccac catcacgatg
    33301 atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg ccagcaagcg atcgtgtaaa
    33361 acttcaactt cacgctgcgg gctatggcaa acgtggaatg tgatactgct atccagtgga
    33421 tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc cgcaacggcg
    33481 cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc tggcgtcaca
    33541 tcgacaaaag cgtccagctc ctggctgctc tccaggtcag aaaggagata aatgtagtcg
    33601 cgcccaagct tcccccatga ggccagcagc gagttgccga catcctgttc accatcgctg
    33661 ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa
    33721 ctgtgtcgac gctgacgagt cagcagtttc gccagataag caggatcttt aatatcgccc
    33781 cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt acccagcgcc
    33841 tggagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa
    33901 ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg ctgatagaga
    33961 ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat attccaccag cgccttccac
    34021 aacggggctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc tgtttcccac
    34081 tgtgccagcc agtccggacg atagaccaga tactggtcaa acaggtccgc cgcttttgag
    34141 gaaagctgga acagttttcg cttgtcgcta tcgtcagtca gataatgccg caacagggta
    34201 aagtcttcgc gctccagcaa ttgcggcagc agagtcatca gtttccagct catgctctgt
    34261 ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc ggacgaacat atcccagata
    34321 aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg cgacagggtc
    34381 atttgcagcc actgcgccat accggtactt tgcaccagaa tcatctctgg ttcgaaagga
    34441 tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac gtccagacga
    34501 ttggaatggt agacccttaa catagcggct cctgactact gacaattcgg gcaatgcagg
    34561 cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat gctgacacat
    34621 cccgcctgcg atgtctgcat tcggttgacc tgccagttgg caggtggcga aatcgcgcgc
    34681 agttgcgttt gctgccaacc atgccgccag agcttttggt actggtttct gctggcgaga
    34741 ctgttcattg atgttcgctg gatacccgat aacgcagtga caatcatcac catcagcacc
    34801 atcagcacca tcagcaccat cagcaccatc gcaaccatta cctccggcag gctaaagcct
    34861 tgttgattct tcagggaagc tgacataatg ccccctcttt cagcggacaa aaatcgctcc
    34921 agccgcgtgg cgaaaaaaca atttttccat caatgacttc gcctgttcgc cacaacgaaa
    34981 cgccttcata accggcaatc aataaggctt cattatctgc cagtaaacgc aaacaaacct
    35041 gggcatcggt tccagcgtac tgcgagcact gaactgctgg ctgcgtctgc caggagtgca
    35101 tttttcccca cgccagcgcc gactgaacga tggcctggcg gcgcaatgac tgactttcca
    35161 tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat tccttgcaat agcaagctac
    35221 ccaaaaccag cagcatcagg accagagcca gtgacgaaac acctttttcg cggttcacag
    35281 gttgaatcct gtcacgctat agctggcatc caccacggtt tgcggttcag acttactggc
    35341 agcacgcata ttaaccgtca acaccggcga gaagccgctg acatcctgac gtacgacctg
    35401 aaaagtgtcg ataatgatgg catccggatt agtgacttta tcccagccct taccttcaca
    35461 ggatgtcgca ccgcgtagcg tttccagcac atgctccttc agacgaaatc caatctggtc
    35521 ggactctttt accggttcgc gatcccagat accgttactg ttcgcatccc actgcacaat
    35581 gatacagtca ccctgtccga caatttccag cccttcgccg gtacagctgc catgacaata
    35641 acccgccctc tggagatgct tcgcgacggt aaatacccgc agccagattt catcttccag
    35701 tgccagctta cgagtgctcg ttaaactttc acgctgtaac gcaggcagaa agcgtgcagc
    35761 ccccagcaac aatacgctac tgatcgccat agcaatcaac acttccagca gagaaaaacc
    35821 ttgctctttt acaggcatcc ttctgtttct ccttgctgat aaagccggag tcttccccac
    35881 ggcgaaacca ccagccacca ctcgcccgtt gagtttttga agcgaatatg cacggcccat
    35941 gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg
    36001 ggccagcgtg gcacaaagac caatggtgaa ctgccatgac aggtattggc cccagcagcg
    36061 gaactcacaa ggcaccataa cgtcccctcc ctgataacgc tgatactgtg gtcgcggtta
    36121 tgccagttgg catcttcacg taaatagagc aaatagtccc gcgcctagct ggcggtttgc
    36181 caaagccgtt gcgactgctg ccagtattgc cagccataga gtccacttgc gcttagcatg
    36241 accaaaatca gcatcgcgac cagcgtttca atcagcgtat aaccacgttg tgttttcatg
    36301 ccggcagtat ggagcgagga gaaaaaaaga cgagggccag tttctatttc ttcggcgcat
    36361 cttccggact atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc gtctcgaaga
    36421 atttaacgga gggtaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt
    36481 aattagatag ccaccggcgc tttaacgccc ggatgtggat cgtagccttc aatctcaaag
    36541 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
    36601 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatggttgct gtacaggtgc
    36661 gtatcgccac cggtccagac aaaatcaccc acttccagat cgcactgctg cgccatcata
    36721 tgcaccagta acgcgtagct ggcaatgttg aacggcaggc cgaggaagac gtcacaggag
    36781 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaatgcatgg
    36841 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
    36901 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag taatctggtc aatatgacga
    36961 ccatctggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccattt
    37021 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagta
    37081 tcgccctgca ggaaccacag cagttcgtgg atgatggaac gcaggtggca acgtttggtt
    37141 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
    37201 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
    37261 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
    37321 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg ccccatgctg
    37381 atgtactgca cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
    37441 atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga cagctcccat tgggcgcggt
    37501 ttacgaatat acaggttgag gataataaac agcaccacac cttccagcag cagttcgtaa
    37561 agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga aaatggattg ccactgcggg
    37621 ttggtttgca gcagcaaaat atcttctgta cgggagcctg ggaacagcat ggcaaacggg
    37681 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
    37741 ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta
    37801 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaagac
    37861 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaaattg
    37921 tagaacagaa cataaccaat acgtcccccg aggaagacgc cgaggaagcc cgcatagagt
    37981 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc
    38041 agccacattg caaaaatgaa ccccaccaga tacatcaggc cgtaccagtg aagcgccacg
    38101 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
    38161 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
    38221 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
    38281 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
    38341 ccgcgacgct ccataaaggc tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc
    38401 agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt
    38461 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
    38521 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac gaagatcgat accatgtatt
    38581 tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcgtaa
    38641 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
    38701 gtgccaacag agatgaaatc gaccctcttt gccagatgcg gcagcataaa taccattgac
    38761 ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
    38821 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg
    38881 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
    38941 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
    39001 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
    39061 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
    39121 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
    39181 ccgatgccat caatacagct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
    39241 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
    39301 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtacc
    39361 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
    39421 agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg
    39481 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
    39541 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
    39601 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
    39661 cgtaaatcac cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
    39721 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
    39781 aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc
    39841 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
    39901 gcttcttcca gcgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc
    39961 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
    40021 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa cagggcagtc
    40081 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
    40141 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
    40201 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
    40261 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
    40321 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
    40381 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
    40441 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
    40501 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
    40561 ggcgtgactt aacctctttt acgtcgataa gcagatgcgt tttgtggttt tggcgtattt
    40621 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
    40681 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
    40741 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgcttaccag ctgcaagaga
    40801 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
    40861 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
    40921 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
    40981 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
    41041 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
    41101 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
    41161 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
    41221 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
    41281 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
    41341 tagacatttt aaaatattca ttattagtca atgcattaca tgtaatttga gtaatgaaaa
    41401 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
    41461 gccgtgttta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
    41521 ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga ggtttttcac agttatccca
    41581 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
    41641 cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa aaagcaatat aaatcagaga
    41701 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggcacataa ttgctgtttg
    41761 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
    41821 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
    41881 cggcacttga cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
    41941 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
    42001 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
    42061 cattcgtttg tgttgcccgg ttaacgggta atagcggggt gacctgggaa accagccatg
    42121 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc
    42181 tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag gaagaccgac
    42241 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
    42301 tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc
    42361 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
    42421 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
    42481 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
    42541 gatgttattt gcatggataa ccgatcctaa cgcttggctt gcgctcggta cgctgacgct
    42601 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
    42661 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
    42721 tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg acaaatccgc tttttacaat
    42781 attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat
    42841 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
    42901 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatcttcag
    42961 cctcgactcg gtgattaccg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc
    43021 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
    43081 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
    43141 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
    43201 ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc
    43261 cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc
    43321 ctgctttcaa gatatataac gtcggtttat aaacagacta ttatcatagg taggccaaat
    43381 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
    43441 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
    43501 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
    43561 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
    43621 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
    43681 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
    43741 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
    43801 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg
    43861 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
    43921 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
    43981 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
    44041 agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcgatc
    44101 tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct
    44161 ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
    44221 aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc
    44281 gctacctgca tctggcggac aaacacgatc tgccgcgtat tgtcaccatt cagaaccctt
    44341 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
    44401 tcgaactgct ggcctattcg tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg
    44461 gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
    44521 agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggccttgatc
    44581 ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
    44641 gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg
    44701 aagacgtatt agctgaaatt gaagcggtac atcaggttta tacttatcct gcaccataaa
    44761 gattaacgcc ggttaaaacc ggcgttaaat attaatgacg gcgctgccag atccacagcg
    44821 ccgttattgc cagcgcaaat agcgcaccga agccaatgcc aatgggcacg accgggatgc
    44881 ctaccattac cgccagcgag taaatgccca gcatcaacaa catggcgctg ttttcgccaa
    44941 ggttttgtac tgcaatcgca ttccccgccc cgacgctttt tttaccccgc tcctgtagca
    45001 acgcattgag cggaacgaca aaaaagcccc ccagcacgcc aatcagcatc aacaaggcat
    45061 aggctggcag cagctcgtgt tgcagggaga aaatcggtac caccacgcca atcaaaatcc
    45121 cggctggcat acagcgtgac acggtttcca gcgtaaccag cttcgctgcc gcacctgcgc
    45181 caaccacgat accaatcgct accatcgcgt tgagataggt cggcgtagcg ttatcggtaa
    45241 tgcccagcgc caccggtacc cacagcacca acaggaaacg cagcgtgaca cccgctcccc
    45301 agaataaact ggtgcccacc agcgaaaaac gcgtttcacc attgcaccat agcgaggtgc
    45361 aggcattcag gaaactgcgg gtcatgttga tgagattcca ggactgcccc ggacgcgccg
    45421 ccagtttggg aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg
    45481 ccagagcgac caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa
    45541 tcgccgctat ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg
    45601 ttaattcgcc gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca
    45661 gcgtatagcc gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca
    45721 ggccgttggc aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa
    45781 acggcgccaa aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact
    45841 ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa
    45901 acgcagagag aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt
    45961 tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg
    46021 acaaagtcag gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc
    46081 ggtacagcaa gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc
    46141 agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc
    46201 gcagtggcat ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc
    46261 gacaccattt cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag
    46321 ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg
    46381 cgaacattct cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg
    46441 ttcattatgt tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac
    46501 aacaggcgtg catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc
    46561 ggtacgttga tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc
    46621 aggccaaatt tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc
    46681 accacatagc gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg
    46741 aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg
    46801 cggtaatgca gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac
    46861 aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg
    46921 gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta
    46981 tggacgacgc cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct
    47041 tccggctgct gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact
    47101 ttgtcggcag tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta
    47161 agttgctccg gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg
    47221 gttttgattt cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc
    47281 atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct
    47341 gcattgggca gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt
    47401 ccaacaaaca gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct
    47461 tcgacacatt tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga
    47521 gtttcacgcg ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca
    47581 gcgattttgc gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc
    47641 aaaatatgca gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg
    47701 aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc
    47761 gcgacaaaac ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct
    47821 tcagggaaaa tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc
    47881 ataggttgcg tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac
    47941 cactgttggc ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca
    48001 agcaaaatgc catcaataaa agagacgtga ttaggcgtaa ttagtacgcg ctcgcccttc
    48061 agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa
    48121 aagctaaaaa gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat
    48181 tacacgagat aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc
    48241 gcgcaggttg gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat
    48301 acctctggga tcttgattgt ggtctgcacg acgactcttc gccagcgaga aaacgcaaag
    48361 gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt actgttacag attgatgacc
    48421 ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat tcatgtgctc aacacgaagt
    48481 tgacttcacc tatcaatacc tctgggatca ccactttagc aacctgaagc caaacgccac
    48541 cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa aggtttgcaa tctctttttt
    48601 tgtcttactt atttgcgttt ggctcacatt cccacgatga aaacacgcca ccccttgaac
    48661 caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt tacccactaa ggtattttca
    48721 tggcgaccat aaaggatgta gcccgactgg caggcgtttc agtcgccacc gtttcccgcg
    48781 tcattaataa ttcacccaaa gccagcgaag cttcccggct ggctgtgcat agtgcaatgg
    48841 agtctcttag ctatcacccg aacgccaacg cccgtgcgct ggcgcagcag accactgaaa
    48901 cggtcggtct gatcgttggt gatgtttccg atccgttttt cggtgcaatg gtgaaagcgg
    48961 tcgaacaggt ggcttatcac accggtaatt tcttattgat tggcaacggt taccacaacg
    49021 aacaaaaaga gcgtcaggct attgagcaac tgatccgcca tcgctgtgct gcgttggtcg
    49081 tccatgccaa aatgatccca gatgccgatt tagcctcatt aatgaaacaa atgcccggta
    49141 tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg ttgtattgct cttgacgatc
    49201 gttacggtgc ctggctggca acgcgtcatt taattcagca aggtcatacc cgcattggtt
    49261 atctgtgctc taaccactct atttctgacg ccgaagatcg tctgcaaggg tattacgatg
    49321 cccttgctga aagtggtatt ccggccaatg atcggctggt gacatttggc gaaccagacg
    49381 aaagcggcgg cgaacaggca atgaccgagc ttttgggacg aggaagaaat ttcactgcgg
    49441 tagcctgtta taacgattca atggcggcgg gcgcgatggg cgttctcaat gataatggta
    49501 ttgatgtacc gggtgagatt tcgttaattg gctttgatga tgtgctggtg tcacgctatg
    49561 tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac gatggcgacc caggctgccg
    49621 aactggcttt ggcactggcg gataatcgcc ctctcccgga aattactaat gtatttagtc
    49681 cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct ggaggcaagt catcatgcaa
    49741 ccagcgacta accgcagtta aagcaattcc agcgccagta attcttcgat ggtctggcga
    49801 cggcgaatca accgcgcctg accattatca aacagaactt ctggtaacag cggacggcta
    49861 ttgtagttgg atgacattga tgcgccatat gcccctgtat catgcagtac cagataatca
    49921 cctgccttca cttccggcaa ggcgcgggtt tcaacatttc ccccttcctg ctgggtaaag
    49981 acatcgcccg attcacataa cggcccggcg acgacggttt ccaccgttgg cgcgtgttcc
    50041 agagaacgac catcagctgc cagggcactg atatggtggt aactaccgta cattgccggg
    50101 cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt ggcggctacc catttgtttg
    50161 acgctccgca cctgagtaat taatacgcca gactgcgcta ccaggaagcg acccggttca
    50221 atttccagtt tcacagggtg gcccaaatgg cgagcgattt gctcacgcgc ggcattccac
    50281 agaccataat aatgttcggt atcaaccgcc tcttcaccct gttgataagg aatagaaagc
    50341 ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt cgataacctg acgcaccata
    50401 gcaccacaca cctgctccag atgggcataa tcaacgccag aaccaatgtg catgtgaatg
    50461 ccgactagct gcagatgatg acgttgtatc acgtccagtg cggcgggcag atcggtgtac
    50521 cagataccgt gcttgctgtt ttcgccaccg gtattggttt tttggctatg tccgtgacca
    50581 aaccccggat taacgcgcag ccatacccga tgccctggcg aaacctggcc cagttggtcg
    50641 agcgtatcaa cagaacccgc attcaccgga atttgcaatt cactgacgcg ttcaagcgtc
    50701 gcctgatcga taacatctgc cgtaaaaaca atatcatcgg ggtgcgtttg cggattgtaa
    50761 cccgccgcca acgcacgctc tatttcgcct aacgagacgg aatccacttt cacgccctgc
    50821 tcacgcatta agcgcaaaat atgaatattg gaacaggctt tctgtgcaaa gcgcaccact
    50881 tcaaactgtt tcagcgctgc aatctgccga cgaataattt gcgcatcgta gacccacacc
    50941 gggcagccaa attcagcggg caaacgcagc agattttcgg cggtgagatc ggtatcggtg
    51001 ctgaacagtg aatgtggcat aacaaactcc agatgagtgc ttttttatga ttacgccaca
    51061 tcataaaaag aataaaaaat atcgatttat gtcgagtcta tgcaaaaatg atatggatta
    51121 ccggattgcg agagagcgct aatggccgcc gttaacttac gtcatattga aatttttcat
    51181 gcggtaatga ccgccggaag cctgactgag gcagcacacc tgctacacac ctcacagcca
    51241 accgtcagcc gcgaactggc gcgctttgag aaggtgatcg ggctgaaatt gtttgagcgc
    51301 gtacgtggac gattacatcc taccgtgcaa ggactgcgtc tgtttgaaga agtgcaacga
    51361 tcctggtacg gactggatcg cattgtcagc gccgcagaaa gtctgcgcga gtttcgccag
    51421 ggagaactgt ctattgcctg cctgccggtc ttttcgcaat cttttttacc gcagctcctg
    51481 caaccctttc tggcacgtta tcccgatgtc agcttaaata tcgtgcccca ggaatcaccg
    51541 ctacttgaag agtggctctc ggcccagcgt catgatttag gactcactga gacactccat
    51601 acgcctacgg gaacagaacg taccgaatta ctctctttag atgaagtgtg tgtgttacct
    51661 ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg atgattttca gggtgagaat
    51721 tacatcagcc tttcccgtac tgacagctat cgccagttgc tggatcagct atttactgaa
    51781 catcaggtta aacgacgcat gatcgtagaa acccacagcg ccgcgtcagt ctgcgcaatg
    51841 gtacgggcgg gggtaggtat ttcggtggtt aacccgctca ccgcactaga ttatgcggca
    51901 agcggtttag tggtgcggcg gttcagtatt gcggttccgt tcaccgtcag cctgatccgc
    51961 cccctgcacc gcccgtcatc agcgctggtt caggcgttta gtgggcattt acaagcgggg
    52021 ttaccgaaac tggtcacttc tcttgacgct attttgtcgt cagctacgac agcataaaag
    52081 cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac aggcaaaaca ctgcgctctt
    52141 ctggcaccag taaaccaatt tctgtgcagc caaaaatgac gccctgtgcg ccctgttctg
    52201 caaggcgagc aatcacttgc gcataataag cgcgtgacgc ttcggtaaat tgccccagac
    52261 acagttcttc aaaaataatc tgattaattt tcgcccgttc atccgcttca ggaataagac
    52321 aattgatgga aaattgttcc gtcagccgcc cgcgataaaa atcctgttcc atggtgtaac
    52381 gcgtacccag cagcgccaca cgagtcattc cggccccggt aattgcacgt ccggtggcat
    52441 ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat ggcatccgcc actttatgca
    52501 tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca agcgccgcct
    52561 cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct tctatttcat
    52621 gaaaatcgac gctatgtagc agcatttgcg cagagtgaag cccaccaagc cgctgtttaa
    52681 tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt cctcccagca
    52741 aaccaattgt tttcattcct ctctcctgat gtgtgttacc gcaacaaggc tgaataagga
    52801 acgagacaaa cgcctcaacg gccaggtgcc caatctctat taacgaaaaa agggccggat
    52861 gtacagcaca tccggcccgt gaaatcagac gccgatattt ctcaacttct cgcctgccat
    52921 cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt tccggaatga gccagaaagt
    52981 aatacccaca aacgcaatgt tcagcgcagt gtagagccag aacgtaccgg cagcgccaat
    53041 gctatcaagc agtgtcagga aggccgcgcc gataatcata ttcgacaccc agtttgtcgt
    53101 cgtcgaacag gtaataccga aatcgcggca tttcagcggc tgaatttcag agcacaggat
    53161 ccacaccact ggcgcggcgc tcatcgcata accggcaata cacatcatcg tcatgccaac
    53221 agagagccag gacaagccac tggaagccgt accgttatca aactgcatca ggcaatagcc
    53281 cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt ttcagagccg gtttacgacc
    53341 tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac ataaaggtca gtcccacgac
    53401 cagagtcgca atcatctgtt gttctgtggt cgtaaagccc gccattttga agatacgcgg
    53461 cgcgtagtac atgatgatgt tcataccggt aaactgctgc atcgcctgca acaacatacc
    53521 gagaaacaca gcacgacgga cgttacggtt gatcttaaac agtgcccaac cgccctgttt
    53581 taacttcagg ctttcacgaa tttcgttgag ttcttctcgc gctttttccg acgtatcgcg
    53641 cagcatacgc aacacttctt ccgcctcaat atgacgcccc ttttccgcca gccagcgcgg
    53701 gctatttggc aggaagacca ccagaataat cagcagaact gctggtaaag caagaacccc
    53761 caacattgcg cgccagttac cgctataact gaacgctgta tcggataaaa acgccagcac
    53821 gatgccgagt gtgaccatca actggtacat actgatcatc ttaccgcgaa cgttttcact
    53881 tgccatttca gaaagataca gaggagcggt gtaagacgcg atcccgacag caatgcccag
    53941 caccacacga gcggcgatta acatctctac gctggtcgca aaagcggacc ctatagaacc
    54001 gagtacaaac aggatggccc ccgccatcag gctgtattta cgccccaggc ggaacgacag
    54061 ccaaccatta aacagcgcac caattgctgc gccgagcatc atgctactaa ccacccattc
    54121 ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac ggcaacgctc cggcgattac
    54181 gccgatatca agaccaaata acaatcctgc gaccgcagca gctaccgaaa caaacatatt
    54241 catacgccgc gtatcacgca aagaacgtgg cgttaaagca gattccgtat tgatagtaac
    54301 cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa atacgtgaac aattcacaca
    54361 ggtgtcaggt cggaaacagc ataaatatgg attaaattgc tgcgacatgc cgttatgtga
    54421 tggatattcc aattttaaaa ttaagttgaa ttattgagat tattattaac ctcctaattt
    54481 taaagcagat aaaattcata aagttcatta attgataatt gatatggatt atttcataac
    54541 catgatatgg attatgatga tctagagata taaaaaaccc tgccatgcgg cagggtcata
    54601 aaagtaagag gaatgaatta acgcgccagc caaccgccat ccacagcaat ggtataacca
    54661 tttacataat ctgaagcgct ggaggcaaga aacactaccg gccccatcag gtcactcggc
    54721 agtccccaac ggccagctgg aatgcgatcg agaatttccg cgctacgttg ttcatctgcc
    54781 cgcagttgtt gggtattgtt ggtcgccatg taacccggag ctatcgcatt aacattaatg
    54841 ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac ccatcacgcc gctttttgat
    54901 gcggtataag aaggcacacg gatcccgccc tggaaggaga gcattgacgc gatattgata
    54961 atcttgccgc cattgccttg cgcgataaag tgtttcgccg ctgcctgaga catgaagaat
    55021 acgctcttga tattcaggtt catgacatcg tcccagtctt tttcgctgaa ctcgagagcg
    55081 tcttcgcggc gaatcaatcc ggcgttattc accaggatat caatatgacc aaattcagct
    55141 accgcgcgat ccagcagtcc aggaatgcca tcaatctttc gcagatcggc ggtcaggctt
    55201 aaaaaacgac gccccagcgc cgtgacctgc tcgatggttt cagtcggttc aacgatgtta
    55261 atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg ccatcccctg gcccagccca
    55321 gtatcacaac cagtgacgac cgcaacttta ccttggagag aaaatgcact taaaatcata
    55381 acaacacctt attcgtttat gcccacaact agcgcaaatc tttaacgccc acatggtcca
    55441 tatcattaaa gacctggttt tcaccgacca tgccccagat aaaggtataa gctttggttc
    55501 cgacgccgga atggatcgac cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa
    55561 tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac gcaggcgtca tcatccatat
    55621 tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg acacggcatg gtgttccaca
    55681 agttacctgg agccagctcc gtcagcccca tactcaattg acaggtttcc agtacatccg
    55741 ggacaaaata tttgttaatc gtgcgacggt tactggtgag gttatcgcct aacgtgactg
    55801 gagatacttc gtccggtgtg acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat
    55861 tgtaataaaa cttcgccgga gtggcggtat caatactggc aaagacaact tcttttgcac
    55921 ctttaccaac atacagggcg tcgcggtgac cgatttcata gcattgacca tcgacagtaa
    55981 tcgtaccggc accgccaata ttgataacac ctaactcgcg acgttcaagg aaatagctta
    56041 cgccgagttg tttaccaact tccccgccaa cggaaaccgt tttagttatc ggcataatgc
    56101 cgccaacaat aattcggtca atgtggctgt aaaccatggt gtactcatcg gcgacaaata
    56161 ccttttcaac caaaaattca ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac
    56221 cgtggatgct ctgtcttacg tccacatcaa cctccgataa tcagtgaagc gtgaaaatag
    56281 aaaacgatag tgaaacactg tttcgttttc tctataaagg gatgatatgc ccatgggcta
    56341 gggttttcaa ttaaaaataa aacaatgttt tatttttaat ttaaggatta aaaaaagtgc
    56401 agtgtcgatc acgaatatgt catgagaatg gaaaaatgag ggagcccaga aaatgtatgt
    56461 cgacttgcca gccatcatta ttcaacagat taatgacggc agatatttat cggtaaggag
    56521 gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca cgccctggcc cccgccgata
    56581 caaagcgttg ccagcccttt gcgggcatta cgtttcacca tttcatgaac cagagaaacc
    56641 aggattcggc aaccggaagc gcctatcggg tgaccgagtg cgatcgcgcc accattgaca
    56701 ttgacccgac gctcatccca ctcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac
    56761 gcttcattag cctcgataag atcgacatca gccaactgcc agcctacacg ctccaggcaa
    56821 cggcgggtcg catacaccgg cgcaattccc atcaatgccg gatctacacc aacgctggca
    56881 aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc
    56941 atcattaccg cagctgcgcc atcgtttatg gatgatgcat tccccgcggt cacagaaccg
    57001 agactatcaa atgaaggatt taaacgggct aaaccttctg cactggcgtc agtgcgtggc
    57061 tgttcatcgg tatcaacaac caacgtctga ccgttacttt ggatcattac cgggacgatc
    57121 tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg
    57181 taagcatcct gcaactgacg gctgatgcca tattcgcgag ccagattttc ggcggtgaca
    57241 ccaatatgat aatcattgaa ggcatcccac aacccatcat gcaccagact gtcaaccaac
    57301 tggctattgc caagctgttc accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc
    57361 atgttttcct ggccacctgc gatgacaata tcagcctcgc cacactgtat cgcctgagta
    57421 gccagatgca gtgctttaag accggaaccg caaacgtcat taatcgtgat tgcagaaacg
    57481 ctgttaggaa gaccaccttt aatagccgat tgccttgccg gattctgccc tgcccctgca
    57541 gtcaacacct gaccaagaat tacttcatcc accgcatatg caggaacgcc ggtacgttct
    57601 attaacgctt tcacgaccag actaccaagt tccacggcgg aatgacccgc taacgcacca
    57661 cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca caacgtcttt catcacaacc
    57721 tcgcaccaat taacagtgca atagtaaatg attgttaatt acagttatct taattgttta
    57781 aaaaaagtga tttttatcac aaaggaaata tgcctgagca gtagtcagag tcataactgg
    57841 cacgtaaggt ttgcaaccac taacccacca atagatgggt agatagggcg ttaatctccc
    57901 atacttaact tggtttatgg taaattgccc tccattttgt ttaatttgta gatgatacgt
    57961 tcagataatg tctaatattt ggtctaaaga agaaactctg tgaagtttcg cgctctatgg
    58021 cacagccgtt gatgcaggca cgctattcct tcctattcag ttaggttcgg caggggctgt
    58081 ggtcctgttt attactgctc tggtcgcctg gcctttaaca tattggccac ataaagcctt
    58141 atgccagttc atcctctcat caaaaacatc agcaggtgaa gggataacgg gcgcggtaac
    58201 acactactat ggcaagaaga ttggtaatct gattaccacg ctgtacttca tcgccttttt
    58261 tgtcgtcgtg ttgatatatg cagtggcaat taccaactca cttacggaac agctggcaaa
    58321 gcatatggtt attgatcttc gcatccgtat gttggtgagt ctgggtgttg tattaattct
    58381 gaatctcatt tttctgatgg gacgtcatgc cactattcgg gtaatgggat ttttggtatt
    58441 cccattgatt gcctatttct tatttctttc catttacctt gtcggtagtt ggcaacctga
    58501 tctattaaca acccaggtag agttcaatca gaataccctt caccagatat ggatatcgat
    58561 tcccgtgatg gttttcgcct ttagccatac gcccattatt tctacgtttg ccatagacag
    58621 acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa aaaattatga aagtcgctta
    58681 tctcatcatc tgcataagtg tactgttctt tgtctttagc tgcctgcttt ctattccatc
    58741 ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt ttatcggcgc tttctatgct
    58801 gccgaacgcc ccagcatggt tgtcaatttc cgggattatt gtcgcagtag ttgcgatgtc
    58861 gaaatcattc ctgggtacgt actttggcgt tattgaaggt gccacagagg tcgtcaaaac
    58921 aacactacag caggttggtg taaagaaaag tcgtgcattt aaccacgcac tatcaattat
    58981 gttggtatcg ctgattacct tcattgtttg ttgcattaac ccgaacgcga tttcgatgat
    59041 ttacgcgatc agcggcccgc tcattgccat gatacttttc atcatgccta cgctgtcaac
    59101 gtatctcatc ccggcgctta aaccctggcg ttccatcgga aatctgatta cgctgatcgt
    59161 gggtatcctg tgcgtatcgg taatgttctt tagctaatat ccgttgtgga tggttcaaaa
    59221 ttgctacaat tttgaaccat tccatttttc ataatattca tttgttatta ttccgcccta
    59281 tcacttctct cgtcatatcc tagaattatc agttagttaa acacacaaag agatctgaac
    59341 atcttaaata agactattta agatgcataa cttagattcg caagatatct tctggtcatt
    59401 caaaaacaat ttgcgatata gatattctca taccatgaga tagttatctg gaccacgttc
    59461 gaccatgcaa gattaaatca gtgaaacata atcatattta ataacgagaa atgcattttt
    59521 aaatatctta atcagaatta tagcaaatac agattaatcg caatttttcc cagcaaaatt
    59581 acaaaatcaa ggtgtattga catcatcaat caccaatgaa attatgcaat tatatacgga
    59641 tagggaggtt cttaacatgg ggctttgtag tcgttataaa agtcttacat gcaatagttg
    59701 ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt ttgcagtact gcgcaaactc
    59761 gtg
//
LOCUS       DAZOZX010000007        59624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZOZX010000007 DAZOZX010000000
VERSION     DAZOZX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.007"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHUPT_000388"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHUPT_000388"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0818128.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHUPT_000389"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHUPT_000389"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0818129.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHUPT_000390"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHUPT_000390"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0818130.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHUPT_000391"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHUPT_000391"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0818131.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHUPT_000392"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHUPT_000392"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0818132.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHUPT_000393"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHUPT_000393"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0818133.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHUPT_000394"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHUPT_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0818134.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHUPT_000395"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHUPT_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0818135.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHUPT_000396"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHUPT_000396"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0818136.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHUPT_000397"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHUPT_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0818137.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHUPT_000398"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHUPT_000398"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0818138.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACHUPT_000399"
     CDS             complement(15665..16702)
                     /locus_tag="ACHUPT_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0818139.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHUPT_000400"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHUPT_000400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0818140.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHUPT_000401"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHUPT_000401"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0818141.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKKGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="ACHUPT_000402"
     CDS             19444..19884
                     /locus_tag="ACHUPT_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0818142.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHUPT_000403"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHUPT_000403"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0818143.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21544)
                     /locus_tag="ACHUPT_000404"
                     /pseudo
     CDS             complement(20611..21544)
                     /locus_tag="ACHUPT_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHUPT_000405"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHUPT_000405"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0818144.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHUPT_000406"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHUPT_000406"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0818145.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHUPT_000407"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHUPT_000407"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR0818146.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHUPT_000408"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHUPT_000408"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0818147.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHUPT_000409"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHUPT_000409"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0818148.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHUPT_000410"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHUPT_000410"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0818149.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHUPT_000411"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHUPT_000411"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0818150.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHUPT_000412"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHUPT_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0818151.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="ACHUPT_000413"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="ACHUPT_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0818152.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACHUPT_000414"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACHUPT_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0818153.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACHUPT_000415"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACHUPT_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0818154.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACHUPT_000416"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACHUPT_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0818155.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACHUPT_000417"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACHUPT_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0818156.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="ACHUPT_000418"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="ACHUPT_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0818157.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="ACHUPT_000419"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="ACHUPT_000419"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0818158.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="ACHUPT_000420"
     CDS             37412..37681
                     /locus_tag="ACHUPT_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0818159.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="ACHUPT_000421"
     CDS             complement(37684..38043)
                     /locus_tag="ACHUPT_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0818160.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="ACHUPT_000422"
     CDS             38184..38810
                     /locus_tag="ACHUPT_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0818161.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="ACHUPT_000423"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="ACHUPT_000423"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR0818162.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="ACHUPT_000424"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="ACHUPT_000424"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0818163.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="ACHUPT_000425"
     CDS             41650..42315
                     /locus_tag="ACHUPT_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0818164.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="ACHUPT_000426"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="ACHUPT_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0818165.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="ACHUPT_000427"
     CDS             complement(42949..44166)
                     /locus_tag="ACHUPT_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0818166.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="ACHUPT_000428"
     CDS             44300..45349
                     /locus_tag="ACHUPT_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0818167.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="ACHUPT_000429"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="ACHUPT_000429"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0818168.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="ACHUPT_000430"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="ACHUPT_000430"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0818169.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="ACHUPT_000431"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="ACHUPT_000431"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0818170.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="ACHUPT_000432"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="ACHUPT_000432"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0818171.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="ACHUPT_000433"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="ACHUPT_000433"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0818172.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="ACHUPT_000434"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="ACHUPT_000434"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0818173.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="ACHUPT_000435"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="ACHUPT_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0818174.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="ACHUPT_000436"
     CDS             complement(51544..51903)
                     /locus_tag="ACHUPT_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0818175.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="ACHUPT_000437"
     CDS             complement(51900..52175)
                     /locus_tag="ACHUPT_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR0818176.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="ACHUPT_000438"
     CDS             complement(52234..53358)
                     /locus_tag="ACHUPT_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0818177.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="ACHUPT_000439"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="ACHUPT_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0818178.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="ACHUPT_000440"
     CDS             complement(56090..57157)
                     /locus_tag="ACHUPT_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0818179.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57220..57333)
                     /gene="yhiY"
                     /locus_tag="ACHUPT_000441"
     CDS             complement(57220..57333)
                     /gene="yhiY"
                     /locus_tag="ACHUPT_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0818180.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(57523..59145)
                     /locus_tag="ACHUPT_000442"
     CDS             complement(57523..59145)
                     /locus_tag="ACHUPT_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0818181.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..>59624)
                     /locus_tag="ACHUPT_000443"
     CDS             complement(59407..>59624)
                     /locus_tag="ACHUPT_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0818182.1"
                     /translation="NTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
BASE COUNT        14081 a        16446 c        15102 g        13995 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt caacgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctttttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctgacgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtaca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgtc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctaaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc acgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatca cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttc cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcgctt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggcctgaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcctggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatt
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaatatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttat
//
LOCUS       DAZOZX010000008        59385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZOZX010000008 DAZOZX010000000
VERSION     DAZOZX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.008"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            67..294
                     /locus_tag="ACHUPT_000444"
     CDS             67..294
                     /locus_tag="ACHUPT_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818183.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(371..613)
                     /locus_tag="ACHUPT_000445"
     CDS             complement(371..613)
                     /locus_tag="ACHUPT_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0818184.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            966..1886
                     /gene="lpxP"
                     /locus_tag="ACHUPT_000446"
     CDS             966..1886
                     /gene="lpxP"
                     /locus_tag="ACHUPT_000446"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0818185.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2241..2312
                     /gene="ypdK"
                     /locus_tag="ACHUPT_000447"
     CDS             2241..2312
                     /gene="ypdK"
                     /locus_tag="ACHUPT_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0818186.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2377..3615)
                     /gene="alaC"
                     /locus_tag="ACHUPT_000448"
     CDS             complement(2377..3615)
                     /gene="alaC"
                     /locus_tag="ACHUPT_000448"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0818187.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            3992..5478
                     /locus_tag="ACHUPT_000449"
                     /pseudo
     CDS             3992..5478
                     /locus_tag="ACHUPT_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            5493..6227
                     /gene="ypdB"
                     /locus_tag="ACHUPT_000450"
     CDS             5493..6227
                     /gene="ypdB"
                     /locus_tag="ACHUPT_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0818188.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6240..7097
                     /locus_tag="ACHUPT_000451"
                     /pseudo
     CDS             6240..7097
                     /locus_tag="ACHUPT_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(7100..9602)
                     /gene="ptsP"
                     /locus_tag="ACHUPT_000452"
                     /pseudo
     CDS             complement(7100..9602)
                     /gene="ptsP"
                     /locus_tag="ACHUPT_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(9627..10671)
                     /gene="ypdE"
                     /locus_tag="ACHUPT_000453"
                     /pseudo
     CDS             complement(9627..10671)
                     /gene="ypdE"
                     /locus_tag="ACHUPT_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(10671..11087)
                     /locus_tag="ACHUPT_000454"
     CDS             complement(10671..11087)
                     /locus_tag="ACHUPT_000454"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR0818189.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11084..11191)
                     /locus_tag="ACHUPT_000455"
     CDS             complement(11084..11191)
                     /locus_tag="ACHUPT_000455"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818190.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(11152..11754)
                     /gene="ypdF"
                     /locus_tag="ACHUPT_000456"
                     /pseudo
     CDS             complement(11152..11754)
                     /gene="ypdF"
                     /locus_tag="ACHUPT_000456"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(11769..13016)
                     /locus_tag="ACHUPT_000457"
     CDS             complement(11769..13016)
                     /locus_tag="ACHUPT_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR0818191.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13038..13364)
                     /locus_tag="ACHUPT_000458"
     CDS             complement(13038..13364)
                     /locus_tag="ACHUPT_000458"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0818192.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13583..14548)
                     /gene="glk"
                     /locus_tag="ACHUPT_000459"
     CDS             complement(13583..14548)
                     /gene="glk"
                     /locus_tag="ACHUPT_000459"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0818193.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            14752..16008
                     /locus_tag="ACHUPT_000460"
     CDS             14752..16008
                     /locus_tag="ACHUPT_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0818194.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16123..16449
                     /gene="ypeC"
                     /locus_tag="ACHUPT_000461"
     CDS             16123..16449
                     /gene="ypeC"
                     /locus_tag="ACHUPT_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0818195.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16590..17828)
                     /locus_tag="ACHUPT_000462"
     CDS             complement(16590..17828)
                     /locus_tag="ACHUPT_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0818196.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18164..19366
                     /gene="nupC"
                     /locus_tag="ACHUPT_000463"
     CDS             18164..19366
                     /gene="nupC"
                     /locus_tag="ACHUPT_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0818197.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19416..21528)
                     /locus_tag="ACHUPT_000464"
                     /pseudo
     CDS             complement(19416..21528)
                     /locus_tag="ACHUPT_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(21736..21811)
                     /locus_tag="ACHUPT_000465"
     tRNA            complement(21736..21811)
                     /locus_tag="ACHUPT_000465"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21776..21778),aa:Ala,seq:ggc)
     gene            complement(21851..21926)
                     /locus_tag="ACHUPT_000466"
     tRNA            complement(21851..21926)
                     /locus_tag="ACHUPT_000466"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21891..21893),aa:Ala,seq:ggc)
     gene            22147..22506
                     /locus_tag="ACHUPT_000467"
     CDS             22147..22506
                     /locus_tag="ACHUPT_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0818198.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22508..22900
                     /locus_tag="ACHUPT_000468"
     CDS             22508..22900
                     /locus_tag="ACHUPT_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0818199.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(22952..24367)
                     /gene="gltX"
                     /locus_tag="ACHUPT_000469"
     CDS             complement(22952..24367)
                     /gene="gltX"
                     /locus_tag="ACHUPT_000469"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0818200.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            24626..24701
                     /locus_tag="ACHUPT_000470"
     tRNA            24626..24701
                     /locus_tag="ACHUPT_000470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24659..24661,aa:Val,seq:tac)
     gene            24746..24821
                     /locus_tag="ACHUPT_000471"
     tRNA            24746..24821
                     /locus_tag="ACHUPT_000471"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24779..24781,aa:Val,seq:tac)
     gene            24826..24901
                     /locus_tag="ACHUPT_000472"
     tRNA            24826..24901
                     /locus_tag="ACHUPT_000472"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24859..24861,aa:Lys,seq:ttt)
     gene            24932..25057
                     /locus_tag="ACHUPT_000473"
     ncRNA           24932..25057
                     /ncRNA_class="other"
                     /locus_tag="ACHUPT_000473"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            25177..25518
                     /locus_tag="ACHUPT_000474"
     CDS             25177..25518
                     /locus_tag="ACHUPT_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0818201.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(25509..26435)
                     /locus_tag="ACHUPT_000475"
     CDS             complement(25509..26435)
                     /locus_tag="ACHUPT_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0818202.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            26525..27523
                     /locus_tag="ACHUPT_000476"
     CDS             26525..27523
                     /locus_tag="ACHUPT_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0818203.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(27520..27738)
                     /locus_tag="ACHUPT_000477"
     CDS             complement(27520..27738)
                     /locus_tag="ACHUPT_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0818204.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(27740..29755)
                     /gene="ligA"
                     /locus_tag="ACHUPT_000478"
     CDS             complement(27740..29755)
                     /gene="ligA"
                     /locus_tag="ACHUPT_000478"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0818205.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(29826..30812)
                     /gene="zipA"
                     /locus_tag="ACHUPT_000479"
     CDS             complement(29826..30812)
                     /gene="zipA"
                     /locus_tag="ACHUPT_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0818206.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            31042..31803
                     /gene="cysZ"
                     /locus_tag="ACHUPT_000480"
     CDS             31042..31803
                     /gene="cysZ"
                     /locus_tag="ACHUPT_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0818207.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31988..32959
                     /gene="cysK"
                     /locus_tag="ACHUPT_000481"
     CDS             31988..32959
                     /gene="cysK"
                     /locus_tag="ACHUPT_000481"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0818208.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            33343..33600
                     /gene="ptsH"
                     /locus_tag="ACHUPT_000482"
     CDS             33343..33600
                     /gene="ptsH"
                     /locus_tag="ACHUPT_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0818209.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            33645..35372
                     /gene="ptsI"
                     /locus_tag="ACHUPT_000483"
     CDS             33645..35372
                     /gene="ptsI"
                     /locus_tag="ACHUPT_000483"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0818210.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            35413..35922
                     /gene="crr"
                     /locus_tag="ACHUPT_000484"
     CDS             35413..35922
                     /gene="crr"
                     /locus_tag="ACHUPT_000484"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0818211.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(35964..36815)
                     /gene="pdxK"
                     /locus_tag="ACHUPT_000485"
                     /gene_synonym="thiJ"
     CDS             complement(35964..36815)
                     /gene="pdxK"
                     /locus_tag="ACHUPT_000485"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0818212.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            36920..37294
                     /locus_tag="ACHUPT_000486"
     CDS             36920..37294
                     /locus_tag="ACHUPT_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0818213.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            37327..38050
                     /locus_tag="ACHUPT_000487"
                     /pseudo
     CDS             37327..38050
                     /locus_tag="ACHUPT_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(38254..39165)
                     /gene="cysM"
                     /locus_tag="ACHUPT_000488"
     CDS             complement(38254..39165)
                     /gene="cysM"
                     /locus_tag="ACHUPT_000488"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0818214.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(39299..40396)
                     /gene="cysA"
                     /locus_tag="ACHUPT_000489"
     CDS             complement(39299..40396)
                     /gene="cysA"
                     /locus_tag="ACHUPT_000489"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0818215.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(40386..41261)
                     /gene="cysW"
                     /locus_tag="ACHUPT_000490"
     CDS             complement(40386..41261)
                     /gene="cysW"
                     /locus_tag="ACHUPT_000490"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0818216.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(41261..42094)
                     /gene="cysT"
                     /locus_tag="ACHUPT_000491"
     CDS             complement(41261..42094)
                     /gene="cysT"
                     /locus_tag="ACHUPT_000491"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0818217.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(42094..43110)
                     /gene="cysP"
                     /locus_tag="ACHUPT_000492"
     CDS             complement(42094..43110)
                     /gene="cysP"
                     /locus_tag="ACHUPT_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0818218.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(43414..44205)
                     /gene="ucpA"
                     /locus_tag="ACHUPT_000493"
     CDS             complement(43414..44205)
                     /gene="ucpA"
                     /locus_tag="ACHUPT_000493"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0818219.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(44334..45192)
                     /gene="murR"
                     /locus_tag="ACHUPT_000494"
                     /pseudo
     CDS             complement(44334..45192)
                     /gene="murR"
                     /locus_tag="ACHUPT_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            45356..46252
                     /gene="murQ"
                     /locus_tag="ACHUPT_000495"
     CDS             45356..46252
                     /gene="murQ"
                     /locus_tag="ACHUPT_000495"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0818220.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            46256..47680
                     /gene="murP"
                     /locus_tag="ACHUPT_000496"
     CDS             46256..47680
                     /gene="murP"
                     /locus_tag="ACHUPT_000496"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR0818221.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            47685..47771
                     /locus_tag="ACHUPT_000497"
                     /pseudo
     CDS             47685..47771
                     /locus_tag="ACHUPT_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(47860..48759)
                     /gene="yfeX"
                     /locus_tag="ACHUPT_000498"
     CDS             complement(47860..48759)
                     /gene="yfeX"
                     /locus_tag="ACHUPT_000498"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0818222.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(48855..49430)
                     /locus_tag="ACHUPT_000499"
     CDS             complement(48855..49430)
                     /locus_tag="ACHUPT_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0818223.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(49491..49940)
                     /locus_tag="ACHUPT_000500"
     CDS             complement(49491..49940)
                     /locus_tag="ACHUPT_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0818224.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(49927..50352)
                     /locus_tag="ACHUPT_000501"
     CDS             complement(49927..50352)
                     /locus_tag="ACHUPT_000501"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0818225.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            50566..51435
                     /gene="amiA"
                     /locus_tag="ACHUPT_000502"
     CDS             50566..51435
                     /gene="amiA"
                     /locus_tag="ACHUPT_000502"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0818226.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            51439..52338
                     /gene="hemF"
                     /locus_tag="ACHUPT_000503"
     CDS             51439..52338
                     /gene="hemF"
                     /locus_tag="ACHUPT_000503"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0818227.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQIYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(52344..53396)
                     /gene="eutR"
                     /locus_tag="ACHUPT_000504"
                     /pseudo
     CDS             complement(52344..53396)
                     /gene="eutR"
                     /locus_tag="ACHUPT_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(53442..53942)
                     /gene="eutK"
                     /locus_tag="ACHUPT_000505"
     CDS             complement(53442..53942)
                     /gene="eutK"
                     /locus_tag="ACHUPT_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0818228.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(53955..54614)
                     /gene="eutL"
                     /locus_tag="ACHUPT_000506"
     CDS             complement(53955..54614)
                     /gene="eutL"
                     /locus_tag="ACHUPT_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0818229.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(54624..54692)
                     /locus_tag="ACHUPT_000507"
                     /pseudo
     CDS             complement(54624..54692)
                     /locus_tag="ACHUPT_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            55796..56587
                     /locus_tag="ACHUPT_000508"
     CDS             55796..56587
                     /locus_tag="ACHUPT_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818230.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            57307..57702
                     /locus_tag="ACHUPT_000509"
     CDS             57307..57702
                     /locus_tag="ACHUPT_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0818231.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            57720..57983
                     /locus_tag="ACHUPT_000510"
     CDS             57720..57983
                     /locus_tag="ACHUPT_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818232.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            58006..58548
                     /locus_tag="ACHUPT_000511"
     CDS             58006..58548
                     /locus_tag="ACHUPT_000511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818233.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            58687..58974
                     /locus_tag="ACHUPT_000512"
     CDS             58687..58974
                     /locus_tag="ACHUPT_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818234.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            59013..59219
                     /locus_tag="ACHUPT_000513"
     CDS             59013..59219
                     /locus_tag="ACHUPT_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818235.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        14867 a        15706 c        14614 g        14198 t
ORIGIN      
        1 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
       61 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
      121 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
      181 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
      241 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
      301 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
      361 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
      421 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
      481 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
      541 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
      601 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
      661 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
      721 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
      781 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
      841 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
      901 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
      961 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
     1021 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
     1081 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
     1141 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
     1201 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
     1261 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
     1321 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
     1381 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
     1441 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
     1501 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
     1561 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
     1621 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
     1681 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
     1741 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
     1801 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
     1861 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
     1921 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
     1981 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
     2041 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
     2101 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
     2161 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
     2221 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
     2281 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
     2341 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
     2401 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
     2461 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
     2521 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
     2581 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
     2641 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
     2701 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
     2761 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
     2821 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
     2881 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
     2941 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
     3001 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
     3061 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
     3121 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
     3181 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
     3241 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
     3301 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
     3361 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
     3421 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
     3481 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
     3541 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
     3601 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
     3661 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
     3721 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
     3781 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
     3841 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
     3901 taaatttatg ttttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
     3961 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
     4021 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
     4081 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
     4141 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
     4201 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
     4261 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
     4321 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
     4381 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
     4441 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
     4501 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
     4561 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
     4621 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
     4681 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
     4741 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
     4801 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
     4861 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
     4921 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
     4981 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
     5041 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
     5101 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
     5161 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
     5221 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
     5281 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
     5341 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
     5401 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
     5461 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
     5521 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
     5581 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
     5641 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
     5701 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
     5761 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
     5821 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
     5881 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
     5941 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
     6001 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
     6061 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
     6121 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
     6181 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
     6241 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
     6301 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
     6361 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
     6421 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
     6481 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
     6541 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
     6601 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
     6661 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
     6721 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
     6781 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
     6841 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
     6901 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
     6961 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
     7021 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
     7081 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
     7141 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
     7201 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
     7261 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
     7321 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
     7381 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
     7441 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
     7501 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
     7561 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
     7621 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
     7681 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
     7741 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
     7801 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
     7861 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
     7921 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
     7981 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
     8041 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
     8101 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
     8161 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
     8221 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
     8281 taatcggctt gtcgcccgcc gcccgccgcc agtagcacct gctggtaggc ttcaaattgc
     8341 tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg gaacagcccg
     8401 acaccttccg cgccgttggc aaacgcgcct ggcgcttcca gagcggtgcc gatattcgcc
     8461 gcaatatcaa tacgcttgtt atcacgggaa taggccagct gcgcggcagc ctgcgcctgt
     8521 tgtttttggc gtttatccgc cagcgtctgc gcgacctgat aataaccgct caccgcgtca
     8581 ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg cccggcataa
     8641 cgggcaatcg catccagcgg caagccactc agtaccggga tcgccgaggc acgggccaga
     8701 atcagtgtat gcgaggtgcg cccggttttc tccagaatca tacccgcaag atttttcaaa
     8761 tcaaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt cggtttttcc
     8821 agcaccagat tgttgcgcgg cttcagttcc ggccaggtga tatgcagcaa ctgttcgctg
     8881 atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc agatagtttg
     8941 gcgcaaacct gctccatatt gctgatgatc gccgccccca gtccctgatg ctgttctgtc
     9001 atcaggcgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa atgagcgctg
     9061 aggatagttt tgctttcgcc gtcacgctca cgcaattgct gattcagttg ctcggcaagc
     9121 gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat tgcccgataa
     9181 ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgcc gcttgccagc
     9241 acattgccgt acagtaaatc cgggttaagg cggcttaatg aacgcggcag cggatgcgcc
     9301 gtcagttctg cctgcgtcgg ctgaacgctg tcgctgtcga taaagcgcac caggatgtac
     9361 tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag gctgcaactg
     9421 tcattaaata aggtgccagt tccaatcaac gccagcgagc ttttagcatc agcctttgcg
     9481 ttctggcgat ggttgataaa ggtgatttcg ctttgccact ggctgcactg ttctttaagt
     9541 tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg aatcgttaac
     9601 ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac aaccgtctca
     9661 cgcgtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat gtcgcggcaa
     9721 tcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg gtggcaggcc ccattaccaa
     9781 tgtgggtacg ccagtaccgg ttaagtgcac cgctccaccg tccgtgccgc cgttactgaa
     9841 catatccgcc tgcaacggaa cgccaatttc tgccgccacg gtttcaatcc acgcggtaag
     9901 ttttggcggc gcaatcagcg atttatcgct taacaccagc atcggcccgt taccaatctg
     9961 acgatggtta gccgcgccat aatcaaagtt tttcgcccag caggcggtat caagcacaat
    10021 ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg ccgcgtaatc ccacctcttc
    10081 gctggaactt gccaccagcc acacttccgc aggcagttcg gcgtcgtgca gctcacgtag
    10141 taacgtcacc agcagatagc aactgaggcg atcatcaaag gctttgccca tcacccgctg
    10201 gtgagggagg acctgaaaag tggtatcgaa ggtgacgcga tcgcctggac gaattcccgc
    10261 ctgcatcact tcgtcatagg tgcgcgcgcc aatgtccacg cgcatggcgc taacgtcatt
    10321 cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac tcttcacggg tggtgatgcg
    10381 caccggctgc agctggcggg cagccatgcg cacgttgcca accggcagca cgtcaatagc
    10441 gccttcgcgg gagatgctgc gcaccataaa tcccacttcg tccatatggg cgcagatcat
    10501 caccttcgga cctgtcgatt cattgaggcg gatcagcacc gatcccagac catcaaatcg
    10561 cacttctttt tgcaggcgag ccgcttcttc cagcaggatc tgccgcactt cctgttccga
    10621 ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc gataaatcca ttatgcctct
    10681 cccgtgagca acactgtttt cggcatggcg tagagcactt ctgcgccttg cggggtaacc
    10741 agcacaacat cttcgatgcg cacgccccct tgccctagca aataaatccc cggctccacg
    10801 gtcagcaaca tgcctggctg tagcgtcgtg gtgtcccgcg gtgaaaaacg cggatcttca
    10861 tggacttcaa tgccgatagc gtgaccagtg ttatgaccga aatagtcgcc aaatccagcc
    10921 tcggtaatga cccggcgcgc ggcgtcgtca atctgctggc agcgcacgcc ggggcgaatt
    10981 gcagagattg ccgcgagctg tgcctgcagg acaatctgat agacgtcaaa cagcgggtga
    11041 gattcggcgc tcaccccttc gccattcacc agcaaggtgc gcgtcatatc agcagtagcc
    11101 ctgatacagc gcaccaaaat cgagagtgac aaactcgccc gctgcaacaa tcttgtcgct
    11161 ggctttgccg tgcggcagcg ccccgcgcca gccgctggcg acaatggtgt caaaagaggc
    11221 tttttctgcg ccctgctggc gcataaacca ctccagttca gcggctatct cgcgctcgct
    11281 catccccgcc tgaataaagc ggcgaatatg ctccgctccg cgatcggcaa tcccacaggc
    11341 gaggcggatt atctccacct cctctggcgt tttgatttgc cgcagcacat ccggcgtggc
    11401 gctaaccagt ttcgcattga gttcagactt ccagcggtgc gcggtttccc aactcacctg
    11461 ctggccttca aaaccgagcg tttgcaactg ctcatcggca atgatttgat tgacgatagt
    11521 ggtaagcgtg ttcgttgcgt caagcaaatg cagctggtag ccttgcgcac gggcttccac
    11581 ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt tcacggctaa taaccacata
    11641 gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac
    11701 cgcatccagt tgttgcacct taagccagtt gcgcagcgaa gcgagtaatg tcatctggat
    11761 tttccttctt acaggctatc gattaacaat ttgcctttgc ggaacatcat cagacgcagg
    11821 aacaccacca tcagcgcagt aatgattgcc cccagcgcga tccccgccat ataaacgccc
    11881 aagttagtga ccagcggcca tgcccagata gcggattccg ggaaccattg cactgcgccc
    11941 agccagacag ctgcggttga gccgacaatc gcgccgacca tatatgacgg aatagcggtg
    12001 atggggcttt ccagcgcaaa cggaatcgca ccctcactaa tccccataaa ggccaggaac
    12061 atcgcggtct taccctgcgg ataaagttga gcgttgaaca ggcgtttgcc ggttaaacga
    12121 cggtcaataa tggtcgccag acccagacca atcggcggaa taacgatagc gatagaacga
    12181 gcggtgaccg gtaatacgtg gtcagtggta aagctgaagg caacgaaacc tgcggctttg
    12241 ttgatcgggc caccaaggtc gattgctgtc gccgcggcga tccccattgc gtacatcagc
    12301 gcgcccttct cacctgcggc agtcagcaca gtacggatac cgccgttgat ccagccacca
    12361 aagggagtga tgacgtagta catcgccagc atgacaaaaa tggcggaaag aatcggtagc
    12421 aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact ggattttctg gttcatccat
    12481 ttcacaaggt aaccggcaac gatagaaata atcagcgcac caatgaaggt ggacggcacc
    12541 ggcgaagagg tcgcccattg catagtgctg ggatcgaagt tcagcagttg cgtcggctgg
    12601 gtagacatca acccgccgat aaaaccagcc gggaacgcca gtttgccacc gatagagttc
    12661 gccacaaatg cagcaaacat cggaatggca aaaccaaaca acacgccgcc gaaggactgc
    12721 gacagccagg caaatttaag taaggagagg tcgaaaccgg agaatttccc gctattaaga
    12781 gcatccatga tgccgatctc agcaggaatt ttaagccagc tataagcaat cagctggctg
    12841 aaagcgagga tcacaccgcc cataattaag gtcggcacca tgcgtgaaat ccctgacatc
    12901 acatgctgcg ggagttcacc ccagaagctg cttttagagg cctgcggatt cttaactgcc
    12961 gctgccgcac cggatgcgcc aggcacaacg gttgcactgc gttttttaat ggccataaca
    13021 attatccctg tcgaatatta ctgttgttca gaggcaatca tctcttcgat ttctttgatg
    13081 atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg
    13141 aaacgttcgt tatcttccgg ggtaactgcc acggaatgga tgatgatggt cgcttcggcg
    13201 atatcctgcg ccgtcaggcg attctggatg ccgtccgcgc cctgggtttc aattttcact
    13261 tcataaccgg cttctaccgc cgcttcttcc agcacctgag cggccataaa agtgtgagcc
    13321 aggcccattg ggcaggcaca taaggcaatc agtttcttac tcatcgttaa ttcctcgttc
    13381 agtgatagtt gactagtgaa attatgaacg tgcaaaacaa atcgccgtta ccagacaaat
    13441 aatgcattta ctggagaatt aatcccgctg tacggaagtg tgacgcaggt cggccttgtg
    13501 aggggacaca aagaaaaatt gaattaactg tatgatttaa aagattatcg ggagagttac
    13561 ctcccgatat aacaggaagg atttacagaa tgtgacctaa ggtctggcgt aaatgtgcac
    13621 cggaaccgag aagacccgga tagtcatgga cgatgagata caccggaata tcatggacat
    13681 attctttaaa gcgcccttta tcttcaaatg cggcacggaa accggaggct ttgaagaact
    13741 caaggaagcg cggcacgata ccgcccgcaa taaacacgcc gccaaatgtc ccgagattga
    13801 gcgccagatt gccgccaaaa cggcccataa tgacgcaaaa cagcgacaat gcgcggcggc
    13861 aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc ttttggcttg agattttctg
    13921 gcaggcggtt gtcagctttc acaattgcgc gatacaaatt caccagccca gggccagaaa
    13981 gcacgcgctc cgccgaaaca tgaccaattt ccgcacgcaa tatttcgagg ataatggcct
    14041 cttcttcact attcggcgca aaatcaacgt gaccgccttc gcccggcaag cttacccaac
    14101 gcttatcgac atggaccaga tgcgcaaccc caagccccgt tccggcaccg taaaccgcaa
    14161 taggcttacc ttcgaccggt tctgcgccac caaactgaat cagatgctct tttttcagca
    14221 tcgggatcgc catcgataca gcggtaaaat cgttaataat ttccagatgg ctaaaaccga
    14281 gattcttttt catttcggca attgagaacg cccaggtatg gttggtcatt gccacccagt
    14341 caccggtaat tgggcaagcg atggcaatac agccgtcttt cacctcgacc ttatgttctt
    14401 caagataaac gcgaatgacc gcttcgaggc tggggtaatc aagccctgaa taggtcttag
    14461 cctgcgagat ttcaccactg gcaatatcac acagagcaag acgtgcgttg gtgccgccca
    14521 catcaccgac taatgcatac tttgtcattc ttcaactgct ccgctaaagt caaaataatt
    14581 ctttctcaca ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc aggctccctg
    14641 taaatatcga tctgggtcac acaattactt tatcgtttca gcaccaattg cagcgatgcc
    14701 tttttgcaag ctgggcaaac taagtatctg accccgcata aggaatagaa catgctccat
    14761 ccgcgagcca gaaccatgtt gttattatcg ctccccgccg tggcaattgg gattgcgtcc
    14821 agtcttattc tgattgtggt gatgaaaatc gcctcggtat tacagaattt gctctggcaa
    14881 cgactgccgg gaactctggg gatagcccag gattcacccc tctggatcat cggtgtatta
    14941 acgctaacgg gtattgcggt ggggttggtt atccgtttca gccagggtca tgccggacca
    15001 gaccccgcct gtgaaccgct gatcggcgca ccggttccgc cctctgcgct acctagactt
    15061 atcgtagcat taattctcgg tcttgctggc ggcgtcagcc tggggccgga acatccgatc
    15121 atgaccgtca atatcgccct tgccgtcgcc attggcgctc gtctgttacc gcgcgtcaac
    15181 cgaatggagt ggactatttt agcctctgcc ggaaccatcg gcgcgctgtt tggtacacct
    15241 gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg
    15301 gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg cacttactac cggattattt
    15361 ttccatcccc atttttcact gcccattgcg cattacggac agatggaaat gaccgatatt
    15421 ctcagcggtg caattgtcgc ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc
    15481 ttaccacggt tgcacgcgat gatgaatcaa atgaaaaatc cggtgctcgt gctgggtatt
    15541 ggcggattta ttctcggaat tctgggggtt attggtggac cagtttcgct gtttaaaggg
    15601 ctggatgaga tgcagcagat ggtggcaaat caggctttca gcaccagcga ttactttttg
    15661 ctggcggtaa ttaaacttgc cgccctggtc gttgctgccg ccagtggctt tcgcggtggg
    15721 cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt tgatgctgca tgagcacgtt
    15781 cccgccgttc cagcggcaat aaccgtttcc tgcgctattc tcggcatcgt gctggtggta
    15841 acacgcgatg gctggttaag tctttttatg gcggcagtcg ttgtacccaa taccacattg
    15901 ctaccgctgc tctgtatcgt catgcttccg gcatggctgt tattagcagg taagccgatg
    15961 atgatggtca atcgtccgaa gcaacagcca ccccacgata atgtttagca aaaaatgctc
    16021 tcctttatga tttaagagtt atggcgatga tacggggctt tacctccccg taatattgcg
    16081 ttaacaggcc gctgacggct tcgatcgtga aggagaataa caatgttcag gtcactgttt
    16141 ctggcggccg ccctgatggc atttaccccg cttgcagcaa atgcaggtga aatcacccta
    16201 ctgccatcga tcaaattaca aattggcgat cgcgatcatt acggtaatta ctgggacggt
    16261 ggtcactggc gagatcgtga ttactggcat cgcaattatg aatggcgcaa aaaccgctgg
    16321 tggcgtcatg ataatggcta tcatcgtggc tgggataagc gtaaagcata tgagcgtggc
    16381 tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa aaggccgcgg acatgggcac
    16441 cgccattaaa cgcgtcctcc aatggagcac aatgcctgat gcgacgctag cgcgtcttat
    16501 caggcctaca aaccggctct ttaacgcgta gggcggataa ggcgtttacg ccgcatccgc
    16561 caatagtgcc agatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaacaac
    16621 cagatattca gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg
    16681 ctgttcacca gatcgcccat caacttgctg tcactggtga aaatcagcag tggaaccagc
    16741 gccagggcga taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct
    16801 aatcccatca gaatgacaat aaatgacggc aacatggtga ctgtacgacg cacccacagc
    16861 gggatatgga agcgaatgaa tccctgcatc accacctgcc ccgccagtgt ccccaccacc
    16921 gttgaggaca gtccggcagc aaccagactt aacccaaaga ccgttgccgc agcatggctt
    16981 aacagcggtt gcagcgtcag ataagcctca tcaagatcgg caacaccagt atgaccagaa
    17041 aagtggaacg ccgccgcagc tgtagccatc atcaccagat tgacaaaacc ggcaatcgtc
    17101 atggcgatag ccacatccca tttggtggcg gaataacgtt gttgacgcga accgccatgt
    17161 aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt cgcccctaac
    17221 acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac cattccttta
    17281 cccagctgcg ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcggcaaca
    17341 aacaacagta acccgccaat cactttctcc agcggttttt gcccgcgacg ttgcagcatt
    17401 aaaatcagga aagtcgcgat ccccgtcagc accgcgccct gcaacaacga aacaccaaga
    17461 atgagtttaa aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaata
    17521 atttctgcct gaacccaata gaaccacact acgggacgcg gatagtgatc acgaatctgc
    17581 tccgccagat ttttaccggt ggcaatccct agtttggcag agaggatctg aatcagcatc
    17641 gccatcaggt tggcccaaac gacaacccac agtagctgat agccgaagct agcacccgcc
    17701 tgaatattgg tcgcaaagtt accgggatcg atataaccaa tcgccgcaat gaacgcaggt
    17761 cccattaatg cgagcctcat cttacgcgcc gcccgtccgc tgctactctc aacgcgatag
    17821 ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gctaaacgag
    17881 aatgattatc aaattcattt aaatggattg tggtgatttc tctgatagac caagattatg
    17941 attacgaaaa gattgatgac ttgaatgtta tggttatgtt taatgttagc acatttacat
    18001 aactttcagc ttccatacac aacatagcag aaatgtatga cagatcacta tttttgaagc
    18061 ctgtcacagg acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata
    18121 gaatgtgcac ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca
    18181 ttttgtactg gcacttgccg ttgttgtgat tctcgcactg ctggtaagca gcgaccgcaa
    18241 aaaaattcgt atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt
    18301 cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa
    18361 actgctcgga tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg
    18421 cctggcattc ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cactgatcgg
    18481 tattctccag cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc
    18541 caaagtcaac ggtatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg
    18601 tcagtcagaa aactttattg cctataaaga tatcctcggc aaaatctccc gtaatcgtat
    18661 gtacaccatg gctgccacgg caatgtccac cgtgtcgatg tccatcgttg gtgcatacat
    18721 gaccatgctg gaaccgaaat acgtcgttgc tgcgctggta ctgaacatgt tcagcacctt
    18781 tatcgtgctg tcgctgatca atccttaccg tgttgatgcc agtgaagaaa acatccagat
    18841 gtccaacctg cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg
    18901 tttcaaagtt gccattatcg ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc
    18961 gctgaacgca ctgtttgcca ccgttactgg ctggtttggc tacagcatct ccttccaggg
    19021 catcctgggc tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc
    19081 actgcaagtg ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat
    19141 ggatctgcag aaaattgctt ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt
    19201 cctagtttcc ttcgctaact tctcttcaat cgggattatc gcaggtgcag ttaaaggcct
    19261 gaatgaagag caaggtaacg tggtttctcg cttcggtctg aaactggttt acggctctac
    19321 cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa
    19381 aagccgggga tcattccccg gcttttttac gcctgttaat cagctaatgg ctgcgggcga
    19441 ccaatcaaat acccttgcag atattgcacc ccgagcttat gcaatagcgc ctgctgctgt
    19501 tgcgtctcga caaactccgc gaccacactc aatgacttcg ctttcgccag atcggtaatt
    19561 gatctcacaa tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa gacgccatca
    19621 attttgatga tatcagcctg caaacgcttt aaccgttcgt agttggcata tccggtgcca
    19681 aagtcatcaa tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg
    19741 ctttctgcgt tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag
    19801 atgtgataac gcttaaacag acgaataatc cgcccggcaa tattcttttg cagcagcgtg
    19861 agcggcatta aattgactga aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc
    19921 cacttcaaca aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca
    19981 ataaggggca gaaacttatc cggggtcata atgccgccgt catatttcag tcgcgcgagg
    20041 atctcatcat aaccttcacc ctctttgttg cgaattggct gggcgtagag cagcaaatca
    20101 ccctgatcta acgccgtgcg aatggtattc agcaatagca cctgtttagt ggtctgcccg
    20161 gaaaccatct cctctctgct atccagcgcc agcacatgat gatgtgcgca ggattgctcc
    20221 gccagccagc ttaactgccc caacaagggt tgcagggttt cctgatttcc atcaaaacgc
    20281 ccccaggcag caccatagcc catatccagc ccggtattgt tccagtgaat ttgccgacta
    20341 ttcaggatgt taaccatatg ctggagtcgc ccttccgttt ccggcccgct cagcactaac
    20401 agcagttcac tacccggcaa ttgatacaac ttttcgtttt cctgcatcaa cggcagcagc
    20461 gtacggcaaa ttgagcggat acagtgaacg cgcatcatta agccgtaatg acgactcata
    20521 aactcaagat tatcaatgcg caggcagcaa aaactcttgc ccgcctcttg ctccggcgct
    20581 tgttccaacg cacgaaagtt gggtaggagc gttaacggat ctgttagcgc ctgcaaatgc
    20641 cagcggcgat taagccattc actgcgatga taaatgcgca ccatatagag caggcaaacg
    20701 ctaaaggaaa tcagcaccgc gagaataaat gccagcgaat attcggtttc caccccttgc
    20761 aaaaagttct gattgtaatt cagaaggcaa agcgttgaaa cagcccaggt gagatttaaa
    20821 aacggatagc gaagattacc gaccccgagg gtaaagatga tgaagaaaac gggtaccagg
    20881 taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca gaagtagcag cacgcttaac
    20941 gctgccagcc agcttaaggt aaatgcgagt gagatagtag aaaagcatgt tgtaaatcag
    21001 cactgcggtg aaaaggctta gtaaatcaac gaccgtgaaa atggcatccg catcgccgaa
    21061 aaaggtggat atctttagcg ggaaatcaaa gaagctcccc acaagataca tgctgcattt
    21121 gatgccgatc ggcgtcacca aaccaagcca gaacagacgt tgccagacat atcggctggt
    21181 caatccataa cgccaacgcg tccccagctg ccagcgtaat attgcgcagg cacacaacac
    21241 gacaaacgtc tggctgccga gcatgaccgc agtctgtaaa aaagagagat gaaagttcca
    21301 cagattggtg caaaacatcc cgcataaaac gggaacgaca ccgcgccagc caaagataaa
    21361 caagattgac agcattacgc acagtggcat ccacgccaga aagatataac tggaatggat
    21421 aatggcaagt ggcgaaataa aacgggatag ctgaataagt accacggtga gcgtaaacgc
    21481 tagtgtgaat atcttgatat tttttatcag gttatgctcc acaaacatga gtacactact
    21541 ctcactgatc ggggttttca ggcacacaaa taataagttt attcgcatcg cgaagcaaac
    21601 aggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg gcgctaaccc
    21661 ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc cgctttgcag
    21721 cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
    21781 gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca aatttgctgg
    21841 gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag
    21901 cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga cgggcggcat
    21961 catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga
    22021 aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac
    22081 ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata cattcaggga
    22141 attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac cggctatagc
    22201 cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc aaaacctggc
    22261 gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga atatattcgc
    22321 aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga tgcgccgctt
    22381 gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa acagtttaca
    22441 tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat tcgcgatagc
    22501 aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg aatgcctcgg
    22561 tgtggctaaa caaactgtta accgttggac cagagaaaaa ggctggaaaa cggaaaaatt
    22621 tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag tttgcgagtt
    22681 tattcagaac acgccagcct tccataacac accaatgttg atggaagctg aagagagaat
    22741 tgctgaatac gctcctggag cacgcgcccc ggcgtatcga caaatcatca acgcgataga
    22801 taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg aaggaattcg
    22861 caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg gcaggatatt
    22921 atctcctgcc gtttatcttt ttacacgcta attactgctg attttcgcgt tcagcaataa
    22981 aatccagcgc tttgttgata cgctcgatac tgcgggtctt accaattgcg tgaacggtaa
    23041 catccagtgc tggagactgc cccgcaccgg ttacggcgac acgcagcggc ataccaactt
    23101 tacccatacc cacttccagc tcatccgccg tcgcctgaat agcgtgatga acgttttcag
    23161 cggtccagtc agtaatcgcg gccagtttgt cacgaaccac ttccagcggc tgacgcgcta
    23221 ccggacgcag atgttttttc gccgcatcgg catcgaactc agcaaaatct tcgtagaaat
    23281 aacggcagct ctgtgccatc tctttcagcg tcttgcagcg ttcgcccagc agtttcacca
    23341 gatcagccag ctgcgggccg ttacgggtat cgatattttc ctgctcaatg tgccactgta
    23401 agtgagtagc aacatactcc ggcggcagcg cgttaatgta gtgatggttc agccacagca
    23461 gcttgtcggt gttgaacgca ctggcagatt tgctgacggc attcaaagtg aagtatttga
    23521 tcatctcttc acgagtgaag atttcctgat cgccgtggga ccagcccaga cgcaccagat
    23581 agttcagcag tgcttctggc aaataaccgt catcacgata ctgcattacg ctgactgccc
    23641 cgtgacgttt ggacagtttt ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa
    23701 ccggcaccgg cgctttcagg gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat
    23761 ggtcttcgcc acggataacg tgggtgattt ccatatccca gtcatcgaca accacacaga
    23821 agttataggt tggggaacca tcggtacggc ggataataag atcgtccagt tcctggttgc
    23881 tgaactcgat cggaccacgg atctgatcgt caaaaacaac agaaccttcc tgcgggttag
    23941 caaaacgtac aacacacggt tcatcatcag catgatgctc atggctgtgg cggcagcgac
    24001 cgtcataacg cggcttctca cctttcgcca tttgctcttc gcgcagcgct tccaggcgct
    24061 ctttagagca atagcattta taagcagtgc cctcttccag catctgatcg atcaccgcgt
    24121 tgtagcgatc aaaacgtttg gtctggtagt acggaccttc atcccactcc aggctcagcc
    24181 agttcatgcc atccataatg gcttcgatag cttccggcgt ggaacgctca agatcggtgt
    24241 cttcaatacg cagcacgaac tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag
    24301 cagtacgcgc gccgccaacg tgcagatagc ctgttgggct tggcgcgaag cgagttttga
    24361 ttttcatgaa atggccttac gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat
    24421 tgagtgggcg atattctatc actcccgcct gattcctcaa tgtagttcgg gtttctacct
    24481 tacgcattat tgttttcgtt gacaaattgc gcaacacggt ctgttttgcg atcatcttgc
    24541 ttaaatttac gacgaacgaa caatttcttt agaaaatgcg ttgactcatt ttgaactctc
    24601 cctataatgc gactccacac agcgggggtg attagctcag ctgggagagc acctccctta
    24661 caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta tgtagtctcc
    24721 gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc acctccctta
    24781 caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc gttagctcag
    24841 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
    24901 aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatactcaa agattcgaac
    24961 ctgcagcagg tttgagttga gcgcagcgaa acaacggagc cgctcgcggc gacggcccga
    25021 agggcgagcg aagcgagtca tcctgcacga cccaccaatg caaaaaagcg ccctaaaggc
    25081 gcttttttgc tatctgcgat ttgtaagatt gatttttctt ctcaagttcc ttgttcttct
    25141 accgatatta cctcgtacag tgacgaggac tgaacaatgg ccactatctc ttcaacttca
    25201 attccttcca tacaaacaca gagtagtaac agggcatcac aaggtagcga tgttgcttca
    25261 caaatcgcgc ggataagtca gcaaatcatc aaactcactc aacaaattaa agagattgta
    25321 gacactagcg gcagtgctga agacaagcaa aaacaggccg aattgattca acagcaaatc
    25381 accctactgg aaacacaact ggcgcagttg cagaaacagc aggcagaaaa ggcgcaggag
    25441 aaagagcagc gtttgtctct gaatgtatcc cttctaaatc ctgttgaaaa aacgactcat
    25501 atcgatatct atatctgata caacggatcc ccttcccgac cagccattag cgcattgccc
    25561 tggtctcgta ccacgtccca taacgcttca gcggcggtgg aaagtgaacg gttcttcctg
    25621 cgcaccagca tcaactggcg ttcaaccacc ggcgtaatgc gcttaaccac cagtgggcta
    25681 ccttcaggca atggtagcgc cagtgcagga agaatactga tgccgatgcc tgcggctacc
    25741 atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa tattcgcctg aataccattg
    25801 cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat
    25861 ttcgctccct gtaatgcctg ccagggtaca taatcttcca cggccaaagc gctatcgcga
    25921 tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac attgcagatc gcccactggg
    25981 cctggatcga tgacgatgcc aaagtcgaca tctccctggc gaatactttc catcacccac
    26041 tgctgcggac gatcatgcag gacaaactga atatctggat agcggcgatg gctttcagca
    26101 atgcattgcg gaataagatg cgcggaaatg gtctggctgg cagcgacccg aacttttccg
    26161 ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc tgttaagttc atccagcagt
    26221 cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt
    26281 ctgtccagca ggcgaacacc agtatgattt tccagctcct tcacactgtg acttactgcc
    26341 gactggctca agccgatacg ctctcctgca cgactaaaac ttttctcctg cgctactgtg
    26401 acgaaaacct ttaattgttt taaagaataa ttcatctgtt ttcttcatga atggatgcaa
    26461 taaatcaatt ttatttctca aactggaaga agcacaatag aaccatcgat catctggagt
    26521 ctttatgaaa ctttttcgta tcctcgatcc tttcacctta accctgatca cggtggtgtt
    26581 gctggcctct ttctttccgg ccagaggcga tttcgtcccc ttctttgaaa atctgaccac
    26641 cgcagctatt gccctgctgt tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat
    26701 tgctggcggt ggtcactggc gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt
    26761 tccgattctg ggtgtactgt ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta
    26821 ctccggtttt ctctacttgt gcattctccc ggctaccgtg cagtctgcaa tcgccttcac
    26881 gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct
    26941 ggggattttc ctttcaccat tgctggttgg tctggtgatg aatgttcacg gtgcaagggg
    27001 cagccttgag caggtcggta aaattatgct gcaactgctg ctgccgtttg tgttggggca
    27061 tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac
    27121 tgaccagacg tccattctgt tggtggttta tacagcgttc agcgaagccg tcgttaatgg
    27181 tatctggcat aaagttggct ggggatcatt gctgtttatc gtggtggtca gctgcgttct
    27241 tctggctatc gtgattgtag ttaacgtctt tatggcacgc cgactgagct tcaataaggc
    27301 agatgaaatt actatcgtct tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat
    27361 ggcaaacatt ctgttcccca catcggtgat cggtatgatg gtgctgcccc tgatgatttt
    27421 ccatcagatc caattgatgg tctgtgcggt gctggcgcgt cgatacaaac gccagaccga
    27481 acagttacag gcgcagcagg aaagcagcgc cgataaagct taaagcggac gcttcagggg
    27541 ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc gtgatttgca ttagctcacc
    27601 gagcttccct gccgggaact catctttgcg ggcaaaccac agcagatact cctccggcag
    27661 gtcaattaag cgacgccctt tgtatttgcc aaacggcatt atcgtattgg ctatttcaat
    27721 cagctgctct ttttccatct cagctaccca gcaaacgcag catttccgct tcgtcgatga
    27781 cttcaatgcc cagttcctgc gccttcgcca gtttagatcc tgcagcttca cccgctatca
    27841 ccagatcggt tttcttcgac acgctgcccg cgactttcgc gcccagttcg accagtcgag
    27901 ctttagcgtc atcacgcgac atctggctta agctgcccgt aagcaccacg gttttaccag
    27961 caaacgggct gtcaatctct tccgcgttga taacgatcgg cgcaggccag tgaacacctt
    28021 ccgccaacag ctcgctgatg acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt
    28081 gggatgcaac gacaatgcca acatcaggca ccttttgcag ctcttcaatc gaagcggctt
    28141 ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc tgcggtggcc tcgccgactt
    28201 cacggatgcc aagtgcatag aggaagcgag caaaggtggt ttctttcgct ttttccagcg
    28261 cgttaaccac gttttgtgcc aattttggcc ccatacgctc cagtccggtc agtttgcctg
    28321 cggtgagttt gaacagatct gccggagtat ggacatattc tttttcaacc agctgatcga
    28381 tgattttgtc gcccattccg tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg
    28441 actctttacg ctgcgcaccg caaatcaggc caccggtaca gcgggcaacc gcttcacctt
    28501 ccacacgctc aacgtcagaa ccacataccg gacaatgcgt cgggaataca acctcacggg
    28561 tatcttccgg gcgttcagaa agcacgacgt taaccacctg cgggatcacg tcgccagcgc
    28621 ggcgaatcac cactttatcg ccaatgcgta aaccaagacg ttcgatttca tccgcattgt
    28681 gtaaggttgc gttactcacc agcacgccag caacatggac aggttccaga cgcgcaacag
    28741 gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc acgcacaaag gtcatctgct
    28801 cctgcgccgg gaatttaaac gcaaccgccc agcgcggggc acgcgcgaca aagccaagct
    28861 gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg
    28921 tcgggcggtc ttcttccact ttgtgataga acgccagcac ttcttccgcc gattcacaaa
    28981 gcgttacccg atcgctgacc ggcacccccc actttttaaa ttgcagtaaa cggccaagat
    29041 gagtatccgg cagctcgcca ccttccagaa caccaacgcc atagcagaaa aaagtgagcg
    29101 gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga accagctgcc gcattacgtg
    29161 ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg
    29221 cctgcggcag gaacacttca ccacgcactt ccagacgcgc cgggatattc tctccgtgca
    29281 gcttcagcgg aatggcgcga atagtacgca cattagacgt gatatcttcc ccggtggtgc
    29341 catcgccacg ggtcgcggca ctgactaaaa cgccattttc atacagaata ctgacggcaa
    29401 ggccatccag cttcagctca cagcaccagg tgactttctc gttgtttttc agacggtcct
    29461 gcacacgttt gttgaaagca agaaagcttt cttcatcaaa aacgttatcc agtgacagca
    29521 ttggtacttc atggcgtatc tggctgaaag ctgccagcgg cgcagcacct acacgttggg
    29581 taggcgaatc aggcgtaatc agttctggat gtttggtttc cagctcgcgc agttcgcgca
    29641 tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc catcacatga taaagatatt
    29701 catgatggca aagcgtcgtt cgcagttctg tcagttgttg ttcgattgat tccatatcgc
    29761 accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa
    29821 gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt cctggtactc gcgcaatttc
    29881 tgcggagtca tcatacggcg ctgatcgtca agcacgacac cgcccacttc atcggcaata
    29941 tgctgcgcag attgcagcat cagcttgaag ttctgcagct cgtcaccgta agacggtacc
    30001 tgcataaaga tagtgacacc cggagtagtg aaatccttca tttcaggatc aaaggttccc
    30061 ggtttcacca tattcgccag gctgaataac gccgggccgc tgccatccgg gctaagatga
    30121 cgatggtaaa tattcatatc gccaaaaatg aagcccgctt gttgaatgct gttaagaagc
    30181 agttcaccgt ttagctcgct accgtgatgc gccgcgacgt tcatgataat caccgcttct
    30241 ttgcgcttcg gtttatccat aactggagcc ggttccgcta caggctcagg ctgtggtgcc
    30301 gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc
    30361 tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca acggctgttc aggctgcggc
    30421 tgataggcag gctgctgcac cggctgcgct ggacgcggag catgttgcgg cggtacctgc
    30481 gcttcaggcg gctgctggac cggttgacgc ggctgcgcag acgcataagg cggtaggtac
    30541 tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg ggcatgattc
    30601 acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc gtcataagaa
    30661 tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat agaagatcgt
    30721 tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat cgcgccaaca
    30781 atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct aacaccttgc
    30841 caccacggca aacatttact cactaagagt atttgccgat tacctcaagt gcaagtgcac
    30901 tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt tttcgaacat
    30961 atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc tgggattggt
    31021 caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg cagcggtttt
    31081 tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt
    31141 ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct ctttacacag
    31201 ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca atggctgagt
    31261 tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta tttcttctcc
    31321 acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca actggaagca
    31381 cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa agatgtgccg
    31441 cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta
    31501 ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact gtggttcctg
    31561 tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa ccacaaagtg
    31621 ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt
    31681 gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc
    31741 gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg
    31801 taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc
    31861 ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct aaatccttac
    31921 ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat acagttaagg
    31981 acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca cgccgctggt
    32041 tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc gtaaccccag
    32101 cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt
    32161 gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga ttgcactggc
    32221 ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa ccatgagtat
    32281 tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa
    32341 aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc cagagaaata
    32401 cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg
    32461 tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg gcgttgggac
    32521 tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca agaccgatct
    32581 tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc tggcaggtga
    32641 agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta tcccggctaa
    32701 cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag ccatttctac
    32761 cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg gagcagctgt
    32821 tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata ttgtggttat
    32881 tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc tcttcactga
    32941 gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca cctttttagg
    33001 tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca gcctgtcgga
    33061 actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac aggaaaagcc
    33121 aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata atgtttaatg
    33181 acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc
    33241 gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga
    33301 ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca gcaagaagtt
    33361 accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt aaaagaagct
    33421 aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag cgcgaaaagc
    33481 ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat ctccgcagaa
    33541 ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga actcgagtaa
    33601 tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt tcaggcattt
    33661 tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac gaaattgtca
    33721 ttgaccggaa aaaaatttct gccgaccagg ttgatcagga agttgaacgt tttctgagcg
    33781 gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg
    33841 gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat gaggagctgg
    33901 agcaggaaat catagccctg attaaagata aacacatgac agctgacgca gctgctcatg
    33961 aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac ctgaaagaac
    34021 gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg ggcctgaaga
    34081 ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac ctgacgccgt
    34141 ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac gcgggtggcc
    34201 gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc gtgggtaccg
    34261 gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc gtaaataatc
    34321 aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt caggagcaag
    34381 tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt acgctggacg
    34441 gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa ggtgcagagc
    34501 gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg
    34561 cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa gcgtgtggct
    34621 cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg ccatacatga
    34681 acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc
    34741 gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat
    34801 tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga
    34861 tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa
    34921 tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta gccaaagaag
    34981 ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca gttgaccgtg
    35041 gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg aacttgatca
    35101 agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt ggtgagcttg
    35161 ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa ttctctatga
    35221 gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc gaagatgcga
    35281 aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg acgctggtta
    35341 acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca atttactgct
    35401 taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga cgacaaaaag
    35461 gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa tatcgaagac
    35521 gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat caaaccaacg
    35581 ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga aaccaatcac
    35641 gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg tatcgacacc
    35701 gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt
    35761 ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa gtctaccctg
    35821 actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact gtccggtagc
    35881 gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa
    35941 atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat caattcatcg
    36001 ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc acggtgcacc
    36061 gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca aaacaggtcg
    36121 ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac gctgtcggca
    36181 ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct ggtaatcacc
    36241 acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact gtcgagatcg
    36301 cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat tccctgcgcc
    36361 agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata aattccgcta
    36421 tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc tttgcgtagc
    36481 gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata gcccgtggtt
    36541 acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac
    36601 cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt gctcagcaat
    36661 accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac cgcaatgctg
    36721 ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc
    36781 ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct tgccgatgat
    36841 cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg ctattacaaa
    36901 acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc tgctaatgac
    36961 actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg ccatgctgga
    37021 gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac atacctgcga
    37081 tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca ttgacactgc
    37141 cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt
    37201 gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt taattgcgca
    37261 gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc atttacagga
    37321 ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt ttggtggata
    37381 gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat tggtaaattc
    37441 catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa attgattgcc
    37501 gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga tctctactat
    37561 tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt ccagtgccat
    37621 tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa
    37681 ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg ctgatttccc
    37741 tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca gtattcttga
    37801 agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga gcaatatgat
    37861 tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc ataaacttaa
    37921 aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg
    37981 tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactg cttttccaca
    38041 cgaaaattaa aaactgcatt tgtcggtagc aacaactgcc gacaaatcag attattgaca
    38101 aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata aaaatattgc
    38161 taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc aggcagtttt
    38221 gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct gactaaaatg
    38281 ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga tgatcgctac
    38341 caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg cgccgcctga
    38401 gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc
    38461 gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc ccggcagata
    38521 ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt gcaggccaac
    38581 aatggtcacc ggtttggatt gttcgcgcat aaagcatgaa acgccggtga tagtgccggt
    38641 cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg
    38701 cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat cgagcagctt
    38761 tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc
    38821 tttggtgaca agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct
    38881 catgttgtcg ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag
    38941 cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc
    39001 gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg
    39061 gttattgcct tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa
    39121 cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacaatct ctatccatac
    39181 gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc
    39241 cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc
    39301 aggcgctttg tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat
    39361 acagccgcgc atgttgcaga ccaacgaata aacgctcgcc atgctgcggg gcatcgtcgc
    39421 catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg
    39481 tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg
    39541 tacggcggct gatatccact tcccacgggc gcaggaagag atccaccggc ccctgatacg
    39601 caggtgtgta gcccagcggc cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg
    39661 ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc
    39721 gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc accactacac
    39781 gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg gtgaatttca
    39841 gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg
    39901 cgccgaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg
    39961 ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc agatgcgcaa
    40021 gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct
    40081 cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc gaataccgtc atatggcgga
    40141 acagcgcgta atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca
    40201 cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatcc
    40261 gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag
    40321 gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttgatattgg
    40381 caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc
    40441 cactgcaaca tactctttaa aaacagggtg ataatcgcca tcaacgtcaa cagcgcggcg
    40501 gcggtaaagg agccgacggt gttgtagtcc tgctccaaca attcaatctg taacggcagc
    40561 gaaagtgttt cgccgcgaat cgagccggaa accaccgaca ctgcaccaaa ctcgccaatc
    40621 gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg
    40681 acgcgacgga acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg
    40741 ctgccctggc ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag
    40801 atggtgacca gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc
    40861 cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc ggcaaccacc
    40921 ggcgatacag caaacggaat gtccagtagc gtcagcagta actggcgtcc cgggaagtta
    40981 aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc
    41041 agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt
    41101 aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc
    41161 aggatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc ccagttaatc
    41221 gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc
    41281 gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga
    41341 tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca
    41401 tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa
    41461 attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagct
    41521 ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt tccgccgctt
    41581 cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc acaaacggaa
    41641 tgctggtaaa ggccatcgcc accgcaatac caagccaggt ataggtgact ttgatatcaa
    41701 acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac
    41761 cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc
    41821 ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa
    41881 tcgatgccac aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg
    41941 tgatcacctc ccagtactgc gcccagctca tctcagaaag ctgcatcacc agcgcagaga
    42001 gcggcagcag caatatcagg cacacaaaca gcagactggt gccaaggctt aaagtaaagc
    42061 ccggcagcac gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt
    42121 gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccatg agccaaattt
    42181 gtcttccacg cggaacagtt cggtctgcgg gaatttatct ttcagtttgt ccatgacttc
    42241 cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag
    42301 ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat ttttatcgac
    42361 ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt caaagccctg
    42421 tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca gcacatcgcc
    42481 caagccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac
    42541 gtttttcagg aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt
    42601 atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt
    42661 cgggaaaatc agcttcacgt cggagcgcac caggtcgttc cagtcgtgga tattcttcgg
    42721 gttgccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag
    42781 gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt
    42841 cacctggtta taagtgacaa cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt
    42901 tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca
    42961 ttgctgttca aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact
    43021 gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag
    43081 tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct
    43141 aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg
    43201 atttggaagt taaaaaggga ataagaactt ggttcaacag gcgtaattgt tgcaggcagt
    43261 ttggacacgg acagcgcgga gaaaccggag cgtacacata gtacgtgagg atttcgagca
    43321 ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc
    43381 tccccacaca tgcagggagg aaacagaggt gaatcagata ccgacgctaa ccgtctccgg
    43441 cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg attcatccga
    43501 tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac acagcgggat
    43561 tgcttttgcc atttcagtca gcaccgattc tggatcttcc gggttcgact ggcgggcaat
    43621 gctttccgcc attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc gaataccgga
    43681 ttgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt tcgttaaggc
    43741 atacgccgtt tcgccaggat cagccaccat atcaccagtg actgaagaca tcatcacaat
    43801 gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc
    43861 tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc
    43921 cagacgacaa acgcctgcgt tattcaccag gatatcaatg cgcccttctt tttccttcgc
    43981 gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca
    44041 gcgatgacca cgaccacaca gttcgtccgc tagcttttcg atctcagggg agatatccag
    44101 caagattagg ttcgcgccat gacgtgcaaa agttctggca attccttcgc caattccctg
    44161 caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa tgctctcctt
    44221 gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca aaatattgct
    44281 gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat gcatcatttc
    44341 aggcgttgtg ttagctcact gctgcgctga atcattttca atgacgccac atcattgatg
    44401 ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc gggtagacat
    44461 cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat gcgccagccg
    44521 ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct gctttcgtgc
    44581 cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag cgatttgtac
    44641 atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc
    44701 cacgcgatag ccaattttca tcagtttgaa cgacagatcg cgccccacca gcgccgatcc
    44761 gcccaggccc gttatctgga taaacggtgc tttactgatg acctcaatga ttttttgcag
    44821 ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc agctcttttt cgcgattcag
    44881 tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc
    44941 cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca tacgcaattc
    45001 agtaaatcct tgcgcgccga gcttctgggc aaatttcaca atgctcgact ggctgatacc
    45061 gagctgtttc gccatctgcc gtgaagagac ggatttcagc tcgctcactc tggcccgcag
    45121 aaagtcagca attttttgct cgttcccggt aaattcactt tccgcattgc gtattttcgt
    45181 caggtacaac atcttccact ctcctaggct ggcagcaaaa attccgctta agttcttatg
    45241 acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt caattcggga
    45301 tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac caccgatgca
    45361 actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg accgcgtatc
    45421 gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc ctgccgttga
    45481 tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg
    45541 cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag
    45601 cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga ttgctggagg
    45661 cgaatatgcc attcagcacg cggtggaagg agctgaagat agccgggaag gtggtgttaa
    45721 cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg ctgccagcgg
    45781 tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct gccgcacagt
    45841 gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg ccattacacc
    45901 gattgtaggt gccgaagttg ttaccggttc ttcgcggatg aaagcaggta cagcgcagaa
    45961 actggtgctc aatatgcttt ccaccgggct gatgattaaa tccggcaaag tgttcggcaa
    46021 cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt
    46081 taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg cctgcgagcg
    46141 caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa
    46201 acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat aacccatggc
    46261 caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa
    46321 tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcact
    46381 ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgacca
    46441 ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct
    46501 gctgggcgat gcaccggtac aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt
    46561 aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac
    46621 gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc
    46681 cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc tgaattttat
    46741 gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt ctggtgggct ataacgccgc
    46801 ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg
    46861 ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct ttggtctgcc
    46921 catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgtgggcct gcgcccgcat
    46981 tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga cctcgttaat
    47041 caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gtggctggtt
    47101 attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg gttgtgcggt
    47161 tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt
    47221 ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc
    47281 aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac cgcacagtgc
    47341 gctacgcagt caggtacgcg gggcgattat tcccggcctg ctgggcgttg gtgaaccgct
    47401 gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc
    47461 ggcgggcggt ttgtttatcg gattgatagc ctggtggggt ctgccgatgg gcttaaacag
    47521 cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac aaggcatcct
    47581 tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg ggtttatttt
    47641 caccacgctc tttggctgcc gtaacgtcaa tctggactga tattatgaaa cggacaatgc
    47701 tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac cctgttctga
    47761 cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg ccagtcagta
    47821 atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcgcca tcaacttgtc
    47881 cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc
    47941 acgtttgcca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg
    48001 cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt acggcaggct
    48061 ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga
    48121 ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat
    48181 catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct cccaacgctg
    48241 cacaaacaca tagctgccgc cggaatccac gccgtctttg ataaccgcca cttcgcggcg
    48301 cgtctcttca cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc
    48361 cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat
    48421 cgccgcctga gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac
    48481 atcgaactgg gtcgtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc
    48541 tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc
    48601 aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact
    48661 ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg
    48721 gcaatgttct ggcaaaatgc cactctgaac ctgagacatt gttcctcctg aaaataagaa
    48781 tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc gacggcggtt
    48841 tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt tttcagggta
    48901 tcgtccgaag gcattaaccc ttcaggacca cgccattccc cgctgaactg gtagctaata
    48961 tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg
    49021 caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg
    49081 tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta
    49141 atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac gttgccgttc
    49201 gtggttttca ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc aatggcttgt
    49261 tcttgcagtg gtgtggacgc cgttaattca cccacgccct gctcgctcac tttggtggac
    49321 gatccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag
    49381 ccggtcagca tcaacggcat agcgcataac attaaacgca gcgatttcat gtcacttcct
    49441 ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt
    49501 tcttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata
    49561 tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac
    49621 ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag cacatgccag
    49681 gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg
    49741 ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta
    49801 ccctgctcac gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat
    49861 agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga
    49921 tggaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg
    49981 ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg gcacgttgat
    50041 ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa gcaacgcatt
    50101 ggcaattcca cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg
    50161 cccgtcataa ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa
    50221 caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg
    50281 cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac
    50341 gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac
    50401 ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt
    50461 caactattga aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa cggattacgc
    50521 gatacgatat aacatctgaa actttattat tacaactcag gccgtatgag cacttttaaa
    50581 ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttagc tgccctgacg
    50641 ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag caacggacac
    50701 agcaagccga aagccaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac
    50761 ggcggaattg ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg
    50821 gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg
    50881 cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc
    50941 gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct
    51001 tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa
    51061 cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg
    51121 cagcaagtac tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgcttggc
    51181 tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa
    51241 gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt
    51301 atcaccaacc cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc
    51361 acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat
    51421 tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac
    51481 cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat
    51541 agttggcagc gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt
    51601 ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc tgcttccgcc
    51661 accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta
    51721 gtgcatccgc ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc
    51781 gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt aacccctttc
    51841 tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgccagcca
    51901 tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa
    51961 catcgcaacg aacagcgcgg cattggcggg ctgttctttg atgatctgaa cacgccagat
    52021 ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta
    52081 ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagatc
    52141 taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac gctgtttggc
    52201 ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg
    52261 gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag
    52321 gtcagggatt gggtgtaact ccctcacccc cattcccgca tccgctgatg caacgtcaac
    52381 gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat
    52441 ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg
    52501 cggcgtacga cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata
    52561 gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca gttgattaca caaatccagc
    52621 accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga aagcaaccga
    52681 cggtaactct gatgactgat gctttccgcc gtcaccattg gttgcgcttc ttccagcatc
    52741 gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga
    52801 ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct
    52861 ttatgctgct ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc
    52921 ggattgtgca aaaagttagc ctgacaggtg atgacatctt cagacagcac tacgcccagg
    52981 atcgtataat cgtccggcgt acttaattca aactcggttc caccggggcg cgtcgctatt
    53041 tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg
    53101 ccaaaccaga acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat
    53161 tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc
    53221 ggatgcagct gatcgtaaat ctgctgctag gcggtaatcg tcaaagcatg ttcatagaca
    53281 tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt
    53341 tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg
    53401 ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga
    53461 taacgactac tgcgtttacg taacgtcccg gcagaaaaga gctgttcgag cgcatttctg
    53521 gctttttcca gcggccagcc aaagtgggcg gccacttctc ctgccgtcat tccctgacat
    53581 actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg
    53641 ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca
    53701 tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat cgcggcagca
    53761 caacctgcgt ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag
    53821 cgaccagggt caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca
    53881 tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc
    53941 atcggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg
    54001 gtaaaggcgt tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag
    54061 tagttggttt cagacggtgg cgggacgtag gtcaccagct gtacgtcggc agatttcaac
    54121 gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg
    54181 ccaagcgtga tcccggcggt tgatgaaaga taagaaccgg tacgcgaaac cacatgcgcc
    54241 aggaaggcgg tgttttctgc gtcattggcc cactggaaag ctgcgccatt ttcaatatgt
    54301 gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc
    54361 agcacttcac cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag cgagcgacca
    54421 tataccactt cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg
    54481 tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc
    54541 gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa
    54601 tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc
    54661 agcatacgtt tcgccaaatc cacaatgacg gcacagcagc acgaaagaga agcaaaagtc
    54721 agctacctgg tacactaggc agcgggtcag caaagggggg caaatgcccc ttttttcatg
    54781 tcagataatc agatcattac ctcaataaac taataatcac atcgcggtta caaccagttt
    54841 gatagtgata gctcaaaccc agacgtggcg ctggctgcaa gcaaaataag ccgcaaaaat
    54901 gcgaccccat gtttactagc cgcaaaaatg cggtcctatt tgccagaacc ttttattatt
    54961 caattggtta gtgcaagaaa aagcgtgatt actgttcaag aagtatccag aaaggtcatg
    55021 attttgacgc tgaaaaaaaa taggtgggca tttttgccca ctcactagcc gcaaatttgc
    55081 ccactcacta gccgcatttt tgcggcccaa agtaataaat acagagaaat aataaataca
    55141 gatcagcgcc aagatttttc gtcttttcgg cggaatcatg agactagtgc ttacgtcctg
    55201 ataacctcca gagcagacct taacttctgt gcagcaggac tacgctcatt gccgctcggc
    55261 taggtcaaat cctcacgata aagctgttcg gattgtgacc ataatgttaa aggactcttt
    55321 aaatcattcc ttgacgaatt aactagctgg cttaagcgga atagcatgca ggataaaaac
    55381 aaaaccagct tagcagtggg gagagcttcg cttcgctcgt ctcctggggc caatgctcaa
    55441 tgaaacgacg taaggcacgg agcgctcacc ataggtagac acataaacat ctgagagcgc
    55501 accttctatc gcgctacgcg ctcaataaaa accaacgaca ccgccacact gcaagaccaa
    55561 tggtttttat cgcgtataca gaccagacag gaaacagcag caaagcagcg tatagccagg
    55621 tgcagacagc acactcacac gacgcacagc aggcttaagt gatcactcag gaaactttat
    55681 ccccgccgcc gatcaccgcg aactatcatg accgcgccaa attcgaaagg aatggcccat
    55741 agaacgcgat gaacacacat aagcgctaca cttacttaac gtcgccacag agcgattgag
    55801 agcgcttaaa atcgatgcga tttcattaaa tttttctcac tcgcctgaat ggtggcgatc
    55861 aggactgatc gcaacattgc cccgcctcga tattgagcgc ataatagata catacattaa
    55921 gcggaaaaga gataagttta ttgtgaaaat cagggggtta catatcatgc gcatatcaga
    55981 acaacaacgg cagatcctcg actatctggc aaaaaacgaa tcaatcaccg cacgacaagc
    56041 cgcagatctc gtttatggtg gcaatgttac ccgcgtccag gttgacgccg cccgccgctc
    56101 actcatgacg atggttagta atgggctaat caggaaacaa ggccgcgctt tcgtagctga
    56161 aagacatatc aatcacgctt atagccagga cgttgaagaa ttgcgccgcc agatcctttg
    56221 ggtattcagc aaacagggtc aggagtattt cctttctgaa cacccaaatt tcgccgccaa
    56281 agagttaaca gtaagcggat tgtcgtgcgc actggattat cttgtcggtg aaggttgcgt
    56341 tacttacaag cagctatggc acgccctgaa tacgcttgta gaagatggtt tactcattgc
    56401 cacgcgtgag aacatcgaat actggataat gccgcgttca ggtcgccgca atcatgcacg
    56461 gtatatttca ggcaacagat acagaacgcc ggactttaat ccgacacagg aagatcggga
    56521 ccgtgccgaa atgatgtgca gggaacagga gaaaagaatt atggataagt tattttcgat
    56581 gcagtgaaca caagcgggcg caatgcccgt ttttattgcc ttattccacg cctgacgaat
    56641 caatttgatc acacctgaga aaatgcatag ccagaagtta aggccgttat attgcgattg
    56701 ggagcgtttt aaacggtatc aatttaatta attctgatgt ggtgatttgc aggcaaaaaa
    56761 aacccgccgg agaagcggga aatagagaca tatcgttgta gttattaaca gatcactttg
    56821 aggtcgaggg cggctaaccc tcgctaatat tatcccgcta ttaccttgcg ggtgcaaata
    56881 atcttttatt gctttgatga tttaataaat agcacttttt gctaacaaaa accgctgtca
    56941 atatatggcc aaaaatgtca acagaaaaaa taaggtttat aatattaagt atcacatggg
    57001 taatgggttc agggaatcgg aatccaccgc ctcacggaaa ttcaaaattt gcttcttctt
    57061 tcctccgtgg gcggcttctt tttcgtatcg aggtatttat gaaagcaata acattagaac
    57121 agttcgcagc aatggcgaat gtatcgacca gaacgctgag gcgctggcta aaaaaaccat
    57181 caaccggagt ccctttgcca tttaccccac gcggtactcg ccaattattc tgggagcacg
    57241 aatgcaaaga atggttgtac agaacagaac gataataatc acgcatcaca ttttgaggta
    57301 aacatcatga ccaatatcga aagattagaa cgctgtgccg atattatgcg acgcagatgg
    57361 atttatgacc ctgaagcggg tttattgttt agccgtgaaa caaaagacgt tatcaaaggc
    57421 agcatgacca ataaagggca tttggttgta acggtacatg aaggtaaatt caaagtgaca
    57481 ctcacctatc agaaagcctg ctatgtatac acatacggcg cttatgacga gacattatat
    57541 gaagtgcacc acgttaattt gaacaaacag gataaccgca tcaagaatat tcgcctgatg
    57601 ccgaaagaaa agcaccgccg gatacataaa aacgtccgca aacttattcg gctgggccac
    57661 acacaatggc ttcaaccgca attaattgaa caatcactat aaacacagga gtaaatacca
    57721 tgcattcatt agacagttat tttcagcgca cgacagcccc taaatcagcc gcccaggaac
    57781 gccgtgagga attccatgag aaagtgatgc gttctgctga ttatattgcg gacaagttcg
    57841 tggaaacggt tcgtccactt gttgatgaag tagctgataa acttcagtct gaaatgccgg
    57901 aagatatgga aggcactgca aagcggcgac tcatttgcga attaagtcgt cgttttggcg
    57961 tgtcaataag cgcttttaaa taatcgaaaa aaggggggaa agataatgga cgcatttgag
    58021 gtactggcaa ataaaggcgc tgaactggta gcacagcgtg ataaaaccgc caacgaaggg
    58081 gaacgcgcac tgctaaataa acagattaaa gcgatccgta tggcccagtt taaattaatc
    58141 agtaatgaag ttatcgaaac aattaaaccg cagatcccac aaatcgttgc tgacgctatt
    58201 aaagccgccg gactggagaa acgcattgcc gggctaaaaa cgaacgcaga gaaaatggag
    58261 atgtacaaac agagcggtgc tccgaatccc gatgagtact tctcaccgga tgttgagttt
    58321 atggcgcagg ttgaggaacg tctgggtagc tgtctcaccg aggaacagcg tcgctatttt
    58381 gatggagtgg atagttcggc aggtattgac ctgaactcgt atttcggtcg tgaaattgag
    58441 cattttgatg ccgccccgtc tatgccggag ccagaaccag aaatggccac cgatcctgaa
    58501 gctgagcaac ggaaacgcgt attcaatgcg atagggattc ggtactgatc ggataaaaga
    58561 aagccgggaa ttaccccggc tgacttaaca atgtgaccaa tgaacaaatc ggacaagaca
    58621 aacaggataa gcagtctcga aacaatgttc gggaactata ttagcacaag tccggagaaa
    58681 atcatcatgt tggcactacg catcatgcag ggcatcgcta agaccctcgc agaacatgtc
    58741 ttagacctga aacattcgcc actgagcaaa caggccatga agcgccaaac gcttcgtctg
    58801 tgggctgaat attcgcttgg aactatcaat aaaatcatcg acatgaaatc ggggccgagc
    58861 aaccagagcg cggaggaaat ggagttcatc aggcgtttaa ttctcattcg cagagacatt
    58921 catagccagt tacacagcgt gggaatcgat attaatgatg ggacgggcga ttaattttcg
    58981 gacaagtact atgcgcgcgc gaggagctga acatgaagca agatattgca gacaggctgg
    59041 agatactcga aggccagcgg gcagaagcga aacaactacg taagcaggcc agaagagcgc
    59101 acaggaacaa cgaagcagag ttactaacca aatacatcag tttcaccaat tactgcatct
    59161 acgagtgtta caaggaagat gcagaggact ggctggattc attgccagaa cagtactgag
    59221 cagaataaca ccacgtcatg ttgttctgag gtgagcatat cacctctgtt caggtggcca
    59281 aatttagtgt gccacttcag catcaccgtt gaaatcacaa taacgatttt tttgtaaata
    59341 tgctctcaca tatgtaggaa tagaccaggt tggcattgaa acaca
//
LOCUS       DAZOZX010000009        58556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZOZX010000009 DAZOZX010000000
VERSION     DAZOZX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.009"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            130..2664
                     /gene="mrcB"
                     /locus_tag="ACHUPT_000514"
     CDS             130..2664
                     /gene="mrcB"
                     /locus_tag="ACHUPT_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0818236.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2884..5127
                     /gene="fhuA"
                     /locus_tag="ACHUPT_000515"
     CDS             2884..5127
                     /gene="fhuA"
                     /locus_tag="ACHUPT_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0818237.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5178..5975
                     /gene="fhuC"
                     /locus_tag="ACHUPT_000516"
     CDS             5178..5975
                     /gene="fhuC"
                     /locus_tag="ACHUPT_000516"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0818238.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5975..6865
                     /gene="fhuD"
                     /locus_tag="ACHUPT_000517"
     CDS             5975..6865
                     /gene="fhuD"
                     /locus_tag="ACHUPT_000517"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0818239.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6862..8844
                     /gene="fhuB"
                     /locus_tag="ACHUPT_000518"
     CDS             6862..8844
                     /gene="fhuB"
                     /locus_tag="ACHUPT_000518"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0818240.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8879..10159)
                     /gene="hemL"
                     /locus_tag="ACHUPT_000519"
     CDS             complement(8879..10159)
                     /gene="hemL"
                     /locus_tag="ACHUPT_000519"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0818241.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10325..10372
                     /gene="yadX"
                     /locus_tag="ACHUPT_000520"
     CDS             10325..10372
                     /gene="yadX"
                     /locus_tag="ACHUPT_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0818242.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10384..11804
                     /gene="clcA"
                     /locus_tag="ACHUPT_000521"
                     /pseudo
     CDS             10384..11804
                     /gene="clcA"
                     /locus_tag="ACHUPT_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
     gene            11828..11893
                     /gene="yadW"
                     /locus_tag="ACHUPT_000522"
     CDS             11828..11893
                     /gene="yadW"
                     /locus_tag="ACHUPT_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0818243.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            11886..12230
                     /gene="erpA"
                     /locus_tag="ACHUPT_000523"
     CDS             11886..12230
                     /gene="erpA"
                     /locus_tag="ACHUPT_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0818244.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12277..12900)
                     /locus_tag="ACHUPT_000524"
     CDS             complement(12277..12900)
                     /locus_tag="ACHUPT_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0818245.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12938..13738)
                     /gene="btuF"
                     /locus_tag="ACHUPT_000525"
     CDS             complement(12938..13738)
                     /gene="btuF"
                     /locus_tag="ACHUPT_000525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0818246.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13731..14429)
                     /gene="mtnN"
                     /locus_tag="ACHUPT_000526"
     CDS             complement(13731..14429)
                     /gene="mtnN"
                     /locus_tag="ACHUPT_000526"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0818247.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14513..16030
                     /gene="dgt"
                     /locus_tag="ACHUPT_000527"
     CDS             14513..16030
                     /gene="dgt"
                     /locus_tag="ACHUPT_000527"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0818248.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16160..17584
                     /gene="degP"
                     /locus_tag="ACHUPT_000528"
     CDS             16160..17584
                     /gene="degP"
                     /locus_tag="ACHUPT_000528"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0818249.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17739..18895
                     /gene="cdaR"
                     /locus_tag="ACHUPT_000529"
                     /pseudo
     CDS             17739..18895
                     /gene="cdaR"
                     /locus_tag="ACHUPT_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(18984..19370)
                     /locus_tag="ACHUPT_000530"
     CDS             complement(18984..19370)
                     /locus_tag="ACHUPT_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0818250.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19684..20508)
                     /gene="dapD"
                     /locus_tag="ACHUPT_000531"
     CDS             complement(19684..20508)
                     /gene="dapD"
                     /locus_tag="ACHUPT_000531"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0818251.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20539..23211)
                     /gene="glnD"
                     /locus_tag="ACHUPT_000532"
     CDS             complement(20539..23211)
                     /gene="glnD"
                     /locus_tag="ACHUPT_000532"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0818252.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23273..24067)
                     /gene="map"
                     /locus_tag="ACHUPT_000533"
     CDS             complement(23273..24067)
                     /gene="map"
                     /locus_tag="ACHUPT_000533"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0818253.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24435..25160
                     /gene="rpsB"
                     /locus_tag="ACHUPT_000534"
     CDS             24435..25160
                     /gene="rpsB"
                     /locus_tag="ACHUPT_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0818254.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25418..26269
                     /gene="tsf"
                     /locus_tag="ACHUPT_000535"
     CDS             25418..26269
                     /gene="tsf"
                     /locus_tag="ACHUPT_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0818255.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26416..27141
                     /gene="pyrH"
                     /locus_tag="ACHUPT_000536"
     CDS             26416..27141
                     /gene="pyrH"
                     /locus_tag="ACHUPT_000536"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0818256.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27433..27990
                     /gene="frr"
                     /locus_tag="ACHUPT_000537"
     CDS             27433..27990
                     /gene="frr"
                     /locus_tag="ACHUPT_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0818257.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28082..29278
                     /gene="ispC"
                     /locus_tag="ACHUPT_000538"
     CDS             28082..29278
                     /gene="ispC"
                     /locus_tag="ACHUPT_000538"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0818258.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            29467..30225
                     /gene="ispU"
                     /locus_tag="ACHUPT_000539"
                     /gene_synonym="uppS"
     CDS             29467..30225
                     /gene="ispU"
                     /locus_tag="ACHUPT_000539"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0818259.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30238..31095
                     /gene="cdsA"
                     /locus_tag="ACHUPT_000540"
     CDS             30238..31095
                     /gene="cdsA"
                     /locus_tag="ACHUPT_000540"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0818260.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31107..32459
                     /gene="rseP"
                     /locus_tag="ACHUPT_000541"
     CDS             31107..32459
                     /gene="rseP"
                     /locus_tag="ACHUPT_000541"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0818261.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32489..34921
                     /gene="bamA"
                     /locus_tag="ACHUPT_000542"
     CDS             32489..34921
                     /gene="bamA"
                     /locus_tag="ACHUPT_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0818262.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35043..35528
                     /gene="skp"
                     /locus_tag="ACHUPT_000543"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35043..35528
                     /gene="skp"
                     /locus_tag="ACHUPT_000543"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0818263.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35532..36557
                     /gene="lpxD"
                     /locus_tag="ACHUPT_000544"
     CDS             35532..36557
                     /gene="lpxD"
                     /locus_tag="ACHUPT_000544"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0818264.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36662..37117
                     /gene="fabZ"
                     /locus_tag="ACHUPT_000545"
     CDS             36662..37117
                     /gene="fabZ"
                     /locus_tag="ACHUPT_000545"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0818265.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37121..37909
                     /gene="lpxA"
                     /locus_tag="ACHUPT_000546"
     CDS             37121..37909
                     /gene="lpxA"
                     /locus_tag="ACHUPT_000546"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0818266.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37909..39057
                     /gene="lpxB"
                     /locus_tag="ACHUPT_000547"
     CDS             37909..39057
                     /gene="lpxB"
                     /locus_tag="ACHUPT_000547"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0818267.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39054..39650
                     /gene="rnhB"
                     /locus_tag="ACHUPT_000548"
     CDS             39054..39650
                     /gene="rnhB"
                     /locus_tag="ACHUPT_000548"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0818268.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            39687..43169
                     /gene="dnaE"
                     /locus_tag="ACHUPT_000549"
     CDS             39687..43169
                     /gene="dnaE"
                     /locus_tag="ACHUPT_000549"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0818269.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43182..44141
                     /gene="accA"
                     /locus_tag="ACHUPT_000550"
     CDS             43182..44141
                     /gene="accA"
                     /locus_tag="ACHUPT_000550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0818270.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44240..46381
                     /gene="ldcC"
                     /locus_tag="ACHUPT_000551"
     CDS             44240..46381
                     /gene="ldcC"
                     /locus_tag="ACHUPT_000551"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0818271.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46438..46827
                     /locus_tag="ACHUPT_000552"
     CDS             46438..46827
                     /locus_tag="ACHUPT_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0818272.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46892..48190
                     /gene="tilS"
                     /locus_tag="ACHUPT_000553"
     CDS             46892..48190
                     /gene="tilS"
                     /locus_tag="ACHUPT_000553"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0818273.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48239..48499)
                     /gene="rof"
                     /locus_tag="ACHUPT_000554"
     CDS             complement(48239..48499)
                     /gene="rof"
                     /locus_tag="ACHUPT_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0818274.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48486..48686)
                     /locus_tag="ACHUPT_000555"
     CDS             complement(48486..48686)
                     /locus_tag="ACHUPT_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0818275.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48852..49397
                     /locus_tag="ACHUPT_000556"
     CDS             48852..49397
                     /locus_tag="ACHUPT_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0818276.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49394..49816
                     /gene="arfB"
                     /locus_tag="ACHUPT_000557"
     CDS             49394..49816
                     /gene="arfB"
                     /locus_tag="ACHUPT_000557"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR0818277.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49830..50540
                     /gene="nlpE"
                     /locus_tag="ACHUPT_000558"
                     /gene_synonym="cutF"
     CDS             49830..50540
                     /gene="nlpE"
                     /locus_tag="ACHUPT_000558"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0818278.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(50695..51519)
                     /locus_tag="ACHUPT_000559"
     CDS             complement(50695..51519)
                     /locus_tag="ACHUPT_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0818279.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(51572..53290)
                     /gene="proS"
                     /locus_tag="ACHUPT_000560"
     CDS             complement(51572..53290)
                     /gene="proS"
                     /locus_tag="ACHUPT_000560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0818280.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53401..54108)
                     /gene="tsaA"
                     /locus_tag="ACHUPT_000561"
     CDS             complement(53401..54108)
                     /gene="tsaA"
                     /locus_tag="ACHUPT_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0818281.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54105..54509)
                     /gene="rcsF"
                     /locus_tag="ACHUPT_000562"
     CDS             complement(54105..54509)
                     /gene="rcsF"
                     /locus_tag="ACHUPT_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0818282.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(54627..55442)
                     /gene="metQ"
                     /locus_tag="ACHUPT_000563"
     CDS             complement(54627..55442)
                     /gene="metQ"
                     /locus_tag="ACHUPT_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0818283.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55482..56135)
                     /locus_tag="ACHUPT_000564"
     CDS             complement(55482..56135)
                     /locus_tag="ACHUPT_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0818284.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56128..57159)
                     /gene="metN"
                     /locus_tag="ACHUPT_000565"
     CDS             complement(56128..57159)
                     /gene="metN"
                     /locus_tag="ACHUPT_000565"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0818285.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57347..57922
                     /gene="gmhB"
                     /locus_tag="ACHUPT_000566"
     CDS             57347..57922
                     /gene="gmhB"
                     /locus_tag="ACHUPT_000566"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0818286.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            58286..>58556
                     /locus_tag="ACHUPT_000567"
     rRNA            58286..>58556
                     /locus_tag="ACHUPT_000567"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        13920 a        14422 c        16099 g        14115 t
ORIGIN      
        1 tcaccctaac cctctccctc aagggagagg ggaccgatcg agcgcaaatt ttgagagata
       61 tcttcttctg tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg
      121 agaaaaagca tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt
      181 ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat
      241 gatgactatg aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt
      301 aagcctcgtg gcaaacgcgg ctggctatgg ctactgctaa aactggctat cgtttttgcc
      361 gtgctgatcg ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc
      421 aaggtctggc aactgcccgc ggcagtttat ggccgaatgg tcaaccttga accagatatg
      481 accatcagca agaacgaaat ggtgaagctg ctggaggcaa cacagtatcg tcaggtgtcg
      541 aaaatgaccc gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt
      601 ccgtttgatt tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggt
      661 gatcatctgg cgaccatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt
      721 gatccgcgtc tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg
      781 cgcagtggtt tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt
      841 tacgagcatg atggaatcag tctctactcc attgggcgtg cggtgctggc aaacctgacc
      901 gcaggtcgca cggtgcaggg tgcgagtacg ctgacgcaac agctggtgaa aaacctgttc
      961 ctctccagcg agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg
     1021 gacgcgcgtt acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt
     1081 cagagcggcg acaacgaaat ccgcggcttc ccgctggcaa gcttgtatta ctttggtcgc
     1141 ccggtagaag agctaagcct cgaccagcag gcgctgttag tcggtatggt gaaaggggcg
     1201 tccatctaca acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtgctg
     1261 cgtctgctgc aacagcaaca gattattgat caagaactct atgacatgtt gagtgcccgt
     1321 ccgctggggg ttcagccgcg tggtggggtg atctctcctc agccagcctt tatgcagctg
     1381 gtgcgtcagg agttgcaggc aaaactgggc gataaggtaa aagatctctc cggcgtgaag
     1441 atcttcacta cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc
     1501 attccggcac tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc
     1561 gaccgcttta gtggtgaagt tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc
     1621 tacaaccgtg cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat
     1681 ctgacggctt taagccagcc gaaaatctat cgcctgaaca cgtggattgc cgatgcgcca
     1741 atcgcgctgc gtcagccgaa tggccaggtc tggtcaccgc agaacgatga ccgtcgttat
     1801 agcgaaagcg gcagagtgat gctggtggat gcgttgacac gttcgatgaa cgtgccgacg
     1861 gtaaatctgg ggatggcgct ggggctgcct gcggttacgg agacctggat caaactgggc
     1921 gtaccgaaag atcagttgca tccggttccg gcaatgctgc tgggggcgtt gaacttaacg
     1981 ccaatcgaag tggcgcaggc attccagacc atcgccagcg gtggtaaccg tgcaccgctt
     2041 tctgcactgc gttcggtaat cgcggaagat ggtaaggtgc tgtatcagag cttcccgcag
     2101 gcagaacgcg ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg
     2161 gtacaacgtg gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg
     2221 aaaacaggga ctaccaacaa taacgtagat acctggtttg caggcattga cggcagcacg
     2281 gtgaccatca cctgggtagg ccgtgataac aaccagccga ccaaactgta tggagccagc
     2341 ggggcaatgt cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt
     2401 gttccgccag aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc
     2461 ggtggcatgc gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagc
     2521 gagatgcagc agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag
     2581 ccgcaacagc aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc
     2641 aaggatatgt ttggtagtaa ttaacatcta agcgtgaaat accggatggc gagttgccat
     2701 ccggtaaaat aacatcccat ctaagatatt aaccctttct tttcatctgg ttgtttatta
     2761 acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta
     2821 tcttatcttt ataataatca ttctcgttta cgttattatt cactttacat cagagatata
     2881 ccaatggcgc gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta
     2941 gtagccacag cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa
     3001 gacactatca ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca
     3061 actattgcgg cgcggcagtc agctaccggc actaaaaccg atacgccgat tcaaaaagtg
     3121 ccacagtcta tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta
     3181 aaagaagcgc ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc
     3241 tatgaccacc tgatcattcg cggttttgcg gcagaaggcc aaagccaaaa taactatctg
     3301 aatggcctga agttgcaggg caacttctat aacgatgcgg tcattgaccc gtatatgctg
     3361 gaacgcgctg aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc
     3421 ggtctgttga atatggtcag caagcgtccg accaccgaac cgctgaaaga agttcagttt
     3481 aaagccggta ctgacagcct gttccagact ggttttgact ttagcgatgc gttggatgat
     3541 gacggtgttt actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa
     3601 gggtcagaag agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa
     3661 accaatttca ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg
     3721 ttgccgaaag agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt
     3781 aatgaagggg cgaagaacaa cacctattct cgtaatgaga agatggtcgg ctacagcttc
     3841 gatcacgaat ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa
     3901 acctcgcaaa acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc
     3961 aaacagtgtg cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc
     4021 gttgatgatg agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc
     4081 actggcgata tcgaccacac cctgctgacc ggtgtcgact ttatgcgtat gcgtaatgac
     4141 atcaacgcct ggtttggtta cgacgactct gtgccactgt tcaatctgta caatccggtg
     4201 aatcccgatt tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg
     4261 aataaacaga aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg
     4321 gtcaccctag gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgctggg
     4381 acgaccgata aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt
     4441 gataatggtg taacacctta cttcagctat agcgaatcgt ttgaaccttc ttcgcaagtt
     4501 gggaaggatg gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa
     4561 tatgtaccgg aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc
     4621 aacaacctga tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc
     4681 gcacgtggcg tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt
     4741 tcttatactt acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct
     4801 gcacaggtgc caaaacacat ggcttcgctg tgggctgact ataccttctt tgacggtccg
     4861 ctttcaggtc tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg
     4921 gctaactcct ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg
     4981 gcgcgagtcg gcatggcggg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt
     5041 gaatacgtcg ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc
     5101 gttgcaaccg caacctttcg tttctaattt ctcttttggg gcacggattt ccgtgcccat
     5161 ttcacaagtt ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc
     5221 gtaatatctc ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc
     5281 ctgccgggaa agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca
     5341 aaatgcttgg tcgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc
     5401 tggaaagctg gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc
     5461 ctccggcaga agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg
     5521 gcgcgctggg gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg
     5581 ttggcttaaa accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcagc
     5641 gggcgtggat cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga
     5701 cttcagcgct ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc
     5761 aggaacgtgg cctgacggtc attgccgtgt tgcacgatat caatatggcg gcacgctact
     5821 gtgattatct ggtcgccctg cgcagtggtg aaatgattgc tcagggaacg cctgcggaaa
     5881 ttatgcgcgg cgaaaccctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc
     5941 cggcgggtgc tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg
     6001 ccgtcgactg ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca
     6061 cgcggcggct attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact
     6121 gctggcgctc ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt
     6181 cagcgaacca ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct
     6241 tgaactgctg accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc
     6301 atcagaaatg ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca
     6361 tccgttggcg atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag
     6421 cgcagcggaa acgcatttag cgcaatacga agactttatc cgcagcatga aaccccgctt
     6481 tgtgaagcgt ggtgcacgcc cgttactgct gacgacgctt atcgatccgc gccatatgct
     6541 ggtcttcggt ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc
     6601 ctggcaaggg gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcagc
     6661 gtataaagac gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct
     6721 aatggcaacc ccgctgtggc aggccatgcc gtttgtccgt acagggcgct ttcagcgcgt
     6781 acccgcagtc tggttttatg gtgcaacgct ttcggcaatg cactttgtgc gtgttctgga
     6841 taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc ttttcccggc gttattgctg
     6901 gcgctgttag tgattgtcgc tacggcgctc acctggatga acttctcaca ggcgctgccg
     6961 cgcagtcagt gggcgcaggc cgcctggtcg ccggatattg acgtcatcga gcagatgatt
     7021 tttcactaca gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc gggcctgggg
     7081 ctggtgggcg tgctgtttca gcaagtactg cgtaacccgc tggcggaacc gacgacgttg
     7141 ggcgttgcaa caggcgcgca actggggatt accgtcacca cgctctgggc gatccctagc
     7201 gcgatggcga gccagtttgc tgcgctggca ggggcttgcg ttgttggctt aattgtcttt
     7261 ggcgtcgcgt gggggaaacg gctgtcgccg gtaacgctga ttttcgcggg gttggtagtg
     7321 agcctttatt gcggcgcaat caatcagtta ctggttatct tccatcatga ccagttgcaa
     7381 agcatgtttc tgtggagcac tggaacgctg acgcaaaccg actggggcgg tgttgagcgt
     7441 ttatggccgc agttgctggg cggtgtgatg ctgacgttgc tgctacttcg tccgttaacc
     7501 ctgatggggc ttgatgatgg cgtggcgcgc aatctcgggc tggccttgtc gcttgcgcgt
     7561 ctggcggcgc tgtcgctggc gattgtcatc agtgcgctgc tggtgaacgc tgtggggatt
     7621 atcggcttta tcgggttgtt cgcgccgctg ctggcaaaaa tgctgggggc gcggcgtctg
     7681 ctgccacgac tgatgctggc gtcgttgatt ggtgcgctga tcctctggct ttccgatcaa
     7741 atcatcctct ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg
     7801 atcggtgcgc cgctgctact gtggctgctg ccgcgtttac gcagcattag cgcgccggat
     7861 atgaaggtta acgatcgtgt cgcgactgaa cgccaacatg tgctggcgtt tgccctcgcg
     7921 ggcggcgtgc tgttgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac
     7981 ggctggacgt gggcgagcgg ggcgttgctc gaggatttaa tgccctggcg ctggccgcga
     8041 attatggcgg cgctgtttgc gggcgtcatg ctggcggtag cgggctgtat tattcagcga
     8101 ctgaccggca acccgatggc aagcccggaa gtgctgggga ttagctccgg cgcggcgttt
     8161 ggtgtggtgt taatgctgtt tctggtaccg ggtaatgcct ttggctggtt gttacccgca
     8221 ggcagtctcg gggcggcggt gacgctgttg atcattatga tcgccgccgg acgcggtgga
     8281 ttttccccac accgcatgtt actggcgggg atggcgttaa gtaccgcgtt caccatgctt
     8341 ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac ctggatttcc
     8401 ggttcgacct acaacgcgac cgatgctcag gtctggcgca ccggaattgt gatggtgatt
     8461 ttgctggcga ttactccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat
     8521 accgcgcggg cggtagggat ggcgctgacg ccgacgcgaa ttgcgctgct gctgttagcg
     8581 gcttgcctga cggcgaccgc gacgatgact attggaccgt tgagttttgt tggtttaatg
     8641 gcaccgcata ttgcgcggat gatgggcttt cgacggacga tgccacacat cgtaatttcg
     8701 gcgctggtgg gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt
     8761 ccattccaga tcccggcggg gctgctgtcg acctttatcg gtgcgccata ttttatctat
     8821 ttgctgagaa agcagagccg ttaatagaat gcctcgcccg gtgggcgagg caaaattatc
     8881 acaacttcgc gaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt
     8941 gcgccacaga cataaagcct gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca
     9001 gcatcatatg gaagaaacgc ttaaaacgct cgacgtcaca ggccatcaca tcctgatagc
     9061 acgtcacgga ctcggcgtcg gtaaagaaga taccgaacat accaccgacg tggttaacga
     9121 ccagcggaat tcccgcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca
     9181 gctcatccag cgtttcgtga atgcccggct gcgcgacttc attcagacag gcgaaacccg
     9241 ctgccatcgc aatcgggtta ccggaaagcg tacccgcctg atagaccgga cctgtcgggg
     9301 ccagcgcatc cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga
     9361 tgatttttcc gaggcaggtt aaatctggca ctacggcgaa ataatcctgt gcgccagcta
     9421 gcgctacgcg gaaaccggtc atcacttcat cgatgatcag caacgcgcca aattcgtcgc
     9481 acagcgcacg cagacctggc aggaactctg gcagcggcgg aacacagttc atattgcctg
     9541 ccaccggctc gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta
     9601 cagaggccag atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc
     9661 ccggcgagtt tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt
     9721 cagcgtgacc gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac
     9781 gggccaggcg gatggcgctc atcgtcgcct cggtgccgga gttcaccatg cgcaccatat
     9841 ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggtcggtg
     9901 caccaaagct taaaccacgc tcggcggctt caatcacggc attgcggatc gccggatggt
     9961 tatggcccag caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa
    10021 catcgtacag ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag
    10081 taaaggcgcg aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa
    10141 gattttcaga cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt
    10201 ccatgttatt cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa
    10261 tcctgatttc ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat
    10321 tttcatggaa cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat
    10381 tggatgaaaa ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga
    10441 caactgattc gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg
    10501 gcagtcgtcg gcacgcttgt cgggctggct gcggttgctt ttgacaaagg tgtcgcctgg
    10561 ttgcagaacc aacgtatggg ggcgctggta catactgctg ataattatcc gctactgtta
    10621 accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa
    10681 tacgcgcccg aagcaggggg atcggggatc ccggaaattg aaggggcgct ggaagatcaa
    10741 cgtcctgttc gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc
    10801 ggcggaggca tggggctagg gcgcgaagga ccaaccgtgc agatcggcgg caatatcagt
    10861 cgcatggtgc ttgatatttt ccgcctgaaa ggtgatgaag ctcgccatac gctgctggca
    10921 accggtgctg ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt
    10981 attatcgaag agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt
    11041 attggcgtca ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt
    11101 gacgtcggta aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt
    11161 attatttttg gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg
    11221 ctgcaccgtg tgcacggcgg caatattacc aaatgggtac tgatgggggg ggcgattggc
    11281 ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg
    11341 attcctatcg ctaccgcggg gaatttcagc atgggaatgc tgtgtttatc ttcgtcgctc
    11401 gggtcattac caccttactc tgcttctctt ccggcgcgcc gggcggtatt tttgccccga
    11461 tgctggcgct gggtactgtg ctgggaaccg ctttcggaat ggttgccgtt gagctgtttc
    11521 cgcaatatca cctggaggcg gggacgtttg ctattgccgg aatgggggca ttactggcgg
    11581 catctattcg cgcgccgtta acggggatca ttctggttct ggagatgacc gataactacc
    11641 agctcatttt gccaatgatt attaccggtc ttggcgcaac actattagcg caatttaccg
    11701 gcgggaaacc tctatactcg gcgattcttg cgcgcacgct ggcaaaacag gaagctgagc
    11761 aactggcgcg aagcaaggcc gcatcagcca gcgagaatac ttgaacgaaa taccagggta
    11821 ttagataatg gcgattatta ttgggttaga atttgcccga ttgccgatgt cgtttggagc
    11881 aaaatatgag tgatgacgta gcactgccgc tggagtttac cgacgcagca gccaacaaag
    11941 ttaaaagcct gatcgctgac gaagataacc cgaatctgaa attacgcgtg tatatcaccg
    12001 gtggcggttg cagcggcttc cagtatggtt tcacctttga tgatcaggtg aacgaaggcg
    12061 atatgaccat cgaaaaacag ggcgttggcc tggtggttga tccgatgagc ctgcaatatc
    12121 tggtcggcgg ttccgttgac tacaccgaag gactggaagg ttctcgtttc atcgtgacca
    12181 acccgaacgc gaaaagcacc tgcggttgcg gttcttcctt tagcatctaa tctgttgttc
    12241 atgtgccgga taaactatcc ggcacatctt ctctcttcaa cgcccattct catccagcgc
    12301 aaacgtcggt agcttaagat gccaacgaat agccgccagc cgaatcaata gcgtcacgac
    12361 catgcccatc atactggctg tttccagtgg tacggaaaat gtgtaataag ccgtagcgtg
    12421 gacaataccg ccgataatac aggcagttgc gtagatttct gtacgtaaaa tcatggggat
    12481 ttcgcggacc agtacatcac gaatgatccc gccgccaacg ccagtaatga cgcccataca
    12541 aaccgcgatt aacggaccgg cttccgcatt aaaggcttta ttcacgctaa tgccgacaaa
    12601 caccgccaga ccaacggcgt ccagcaccgg caacatccat tttggtaagc gtcttggctg
    12661 gcgcaccagc acgatggtca gcatgctggt gaccatggca acgaccagat cggtgggatc
    12721 tttcacccaa aataccgggc cgtgatccag cgccatgtcg cgaattgttc cgtcgcctac
    12781 tgcggtaacc acgcccagta ccagaacacc aaaagggtcc atacgcaatt ttccggctaa
    12841 caaaacgccg tagatggcaa ataccgctgt gccgactata tccagccaat agacgagcat
    12901 tgttcaatcc ccactgagca cctgaaaagg tcaggcacta atctacctgt gaaagcgcat
    12961 tacagagctg ttgtgctgcg aggataatac gtgggcttgc acgttcaaac cagtcactcg
    13021 tgagaggaat aacgggaatt ttgagctgtt caccccagta ttgtttgatt ttaggaattt
    13081 ggtccggtcc gcctgtaatg acaatagcct gtggcgagcg tgctaacacc tgttcgcggc
    13141 taacttgcgg ccagggaacc cggctgtctt taaagatgtt ttctccgcca caaacttcga
    13201 gtacctggtt ctgaatcgac tcttttccac tggtaaatgg cggattaatg ccgaattgca
    13261 gaaaaacacg ttttttaggt ttatcagcat attgcgcttt caattgcacg tactgatcca
    13321 gcagggattg cgcggcttgt tcggccttgt ctggttgcgg actccagggg gccagttgac
    13381 gtaacgcatt ggcaatttgt tcaatgcttg tcgcatcgac ccacatcact tttattccca
    13441 gcgaagccag ctggtcaacc tgccgctcgg cattacctcc acgccaggca atcaccagat
    13501 cgggtttcag cgcgacaatg cgttccagat tcatcccctg ccaggtggaa acctgctcaa
    13561 tcttttgcgc ttgtggagga tagtcggaat agctgctgac cccaaccggc gtgatcccgg
    13621 cggcaaaggc aagttcagtg ttggcgggag aaagcgtgat gacgcgcggc gcggcgttga
    13681 gccacagtgg cgcgagaaaa gacagggcga ccagcgccct gaacagtgat ttagccatgt
    13741 gccagtttct gcaccagtga ctcaaccatc aggctggact gtttagcggc aacagccagg
    13801 aactcatcga agctaagatg agactgttga tcggccacgt cggagatggc gcgtacgaca
    13861 acaaacggga cgttgaaatt gtggcagaca tgggcgattg ccgtcgcttc catctctaca
    13921 gcaatggcct gtgggaagtt gtggcggatt ttcgccagac caacagaacc gttgatgaaa
    13981 gcgtcgccgc taacaatcag gccacgtaca gcgttaagat tcagttcggc aatgcaggcc
    14041 tcagcggcag cgatcagttt atcgtcagct ttaaagcctg ccggacagcc tggtaactga
    14101 ccgtattcat aaccaaatgc cgtgacatcc gcgtcgtgat aacgcgcttc gtccgagaca
    14161 acgatatcgc ccactttcag cgttggtgcc aggccaccgg cagaaccggt gttaataatc
    14221 acatctggct tgcagtgttc caacagcaga gtggcaccca gcgccgcagc gactttaccg
    14281 atgcccgatt tcagaagcgc aacctcggtt ccattcagtt ggccggtata gatttcgcaa
    14341 ccgccgagac tgatagtttg acggttttcg attttgtcac gcagcagcgt aacttcttct
    14401 tccattgcac caatgatgcc gattttcata gatttactcg cgataagccc gatttgaagg
    14461 catagtttac catgcgctta cggggaagcg tatttctcac gcgggagagg acatggcaca
    14521 gattgatttc cgaaaaaaaa taaactggca tcgtcgttac cgttcaccgc agggcgttaa
    14581 aaccgaacat gagatcctgc ggatcttcga gagcgatcgc gggcgtatca tcaactctcc
    14641 ggcaattcgt cgtctgcaac aaaagaccca ggtttttcca ctggagcgca atgccgccgt
    14701 gcgcacgcgt cttacccact cgatggaagt ccagcaggtg ggacgctaca tcgccaaaga
    14761 aattttaagc cgtctgaaag agcttaaatt gctggaagca tacggcctgg atgaactgac
    14821 cggccccttt gaaagcattg ttgagatgtc atgcctgatg cacgatatcg gcaatccgcc
    14881 gtttggtcat tttggcgaag cagcgataaa tgactggttt cgccaacgtt tgcacccgga
    14941 agatgccgaa agccagcctc tgactgacga tcgctgcagc gtggcggcac tacgtttacg
    15001 ggacggggaa gaaccgctta acgagctgcg gcgcaagatt cgtcaggact tatgtcattt
    15061 tgaggggaat gcacaaggca ttcgtctggt gcatacattg atgcggatga atctcacttg
    15121 ggcacaggtt ggcggtattt taaaatatac ccgtccggcg tggtggcgtg gcgaaacgcc
    15181 tgagacacat cactatttaa tgaaaaagcc gggttattat ctttctgaag aagcctatat
    15241 tgcccgcttg cgtaaagaac ttaatttggc gctttacagt cgttttccat taacgtggat
    15301 tatggaagct gccgacgaca tctcctattg tgtggcagac cttgaagatg cggtagagaa
    15361 aagaatattt accgttgagc agctttatca tcatttgcac gaagcgtggg gccagcatga
    15421 gaaaggttcg ctcttttcgc tggtggttga aaatgcctgg gaaaaatcac gctcaaatag
    15481 tttaagccgc agtacggaag atcagttttt tatgtattta cgggtaaaca ccctaaataa
    15541 actggtaccc tacgcggcac aacgatttat tgataatctg cctgcgattt tcgccggaac
    15601 gtttaatcat gcattattgg aagatgccag cgaatgcagc gatcttctta agctatataa
    15661 aaatgtcgct gtaaaacatg tgtttagcca tccggatgtc gagcagcttg aattgcaggg
    15721 ctatcgggtc attagcggat tattagagat ttatcgtcct ttattaagcc tgtcgttatc
    15781 agactttact gaactggtag aaaaagaacg ggtgaaacgt ttccctattg agtcgcgctt
    15841 attccacaaa ctctcgacgc gccatcggct ggcctatgtc gaggctgtca gtaaattacc
    15901 gtcagattct cctgagtttc cgctatggga atattattac cgttgccgcc tgctgcagga
    15961 ttatatcagc ggtatgaccg acctctatgc gtgggatgaa taccgacgtc tgatggccgt
    16021 agaacaataa ccagtctttt gtaaagacga acaataaatt tttacctttt gcagaaactt
    16081 tagttcggaa cttcgggcta taaaacgaat ctgaagaaca cagcaatttt gcgttatctg
    16141 ttaatcgaga ctgaaataca tgaaaaaaaa cacattagca ctgagtgcac tggctctgag
    16201 tttaggtttg gcgttatctc cgctctctgc aacggcggct gagacttctt cagcaacgac
    16261 agcccagcag atgccaagcc ttgcaccgat gctcgaaaag gtgatgcctt cagtggtcag
    16321 cattaacgta gaaggtagca caaccgttaa tacgccgcgt atgccgcgta atttccagca
    16381 gttcttcggt gatgattctc cgttctgcca ggaaggttct ccgttccaga gctctccgtt
    16441 ctgccagggg ggccagggcg gtaatggcgg cggccagcaa cagaaattca tggcgctggg
    16501 ttccggcgtc atcattgatg ccgataaagg ctatgtcgtc accaacaacc acgttgttga
    16561 taacgcgacg gtgattaaag tccagctgag cgatggtcgt aagttcgacg cgaagatggt
    16621 tggcaaagat ccgcgctctg atatcgcgct gatccaaatc cagaacccga aaaaactgac
    16681 cgcaattaag atggcggatt ctgatgcact gcgcgtgggt gattacaccg tagcgattgg
    16741 taacccgttt ggtctgggcg agacggtaac ttccgggatt gtctctgcgc tggggcgtag
    16801 cggcctgaat gctgaaaact acgaaaactt catccagacc gatgcagcga tcaaccgtgg
    16861 taactccggt ggtgcgctgg ttaacctgaa cggcgaactg atcggtatca acaccgcgat
    16921 cctcgcaccg gacggcggca acatcggtat cggttttgct atcccgagta acatggtgaa
    16981 aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa cgcggtgagc tgggtattat
    17041 ggggactgag ctgaattccg aactggcgaa agcgatgaaa gttgacgccc agcgcggtgc
    17101 tttcgtaagc caggttctgc ctaattcctc cgctgcaaaa gcgggcatta aagcgggtga
    17161 tgtgatcacc tcactgaacg gtaagccgat cagcagcttt gccgcactgc gtgctcaggt
    17221 gggtactatg ccggtgggca gcaaactgac cctgggctta ctgcgcgacg gtaagcaggt
    17281 taacgtgaac ctggaactgc agcagagcag ccagaatcag gttgattcca gctccatctt
    17341 caacggcatt gaaggcgctg agatgagcaa caaaggcaaa gatcagggcg tggtagtgaa
    17401 caacgtgaag acgggcactc cggctgcgca gatcggcctg aagaaaggtg atgtgattat
    17461 tggtgcgaac cagcaggcag tgaaaaacat cgctgaactg cgtaaagttc tcgacagcaa
    17521 accgtctgtg ctggcactga acattcagcg cggcgacagc accatctacc tgttaatgca
    17581 gtaatctccc tcaacccctt cctgaatacg ggaaggggtt ctccttacaa tctgtgaact
    17641 tcgccacaac tccatacatc ttcatcatcc tttgggcatt tgcacaatgc cgtacgttac
    17701 gtacttcctt atgctaagcc gtgcataacg gaggacttat ggctggctgg catcttgata
    17761 ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat catcgatacc aatatcaacg
    17821 taatggatgc ccgcgggcga attatcggca gcggcgatcg tgagcgtatt ggtgaattgc
    17881 acgaaggtgc attgctggta ctttcacagg gacgagtcgt cgatatcgat gacgcggtag
    17941 cacgtcatct gcacggtgtg cggcagggga ttaatctacc gttacggctg gaaggtgaaa
    18001 ttgtcggcgt aattggcctg acaggtgaac cagagaatct gcgtaaatat ggcgaactgg
    18061 tctgcatgac ggctgaaatg atgctggaac agtcgcggtt gatgcacttg ttggcgcagg
    18121 atagccgttt gcgggaagaa ctggtgatga acctgattca ggcagaggag aatactcccg
    18181 cacttctgaa tgggcgcaac ggctggggat cgatctcaat caaccgcgag tggtggctat
    18241 tgttgaggtc gacagcggtc agcttggcgt ggacagcgca atggcggagt tacaacaact
    18301 gcaaaacgcg ctgactacgc ccgagcgtaa taatctggtg gcgattgtct cgctaaccga
    18361 aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag aagatcatcg
    18421 taagcgagtt gaacaactga ttacccgcat gaaagagtac ggccagctgc gttttcgcgt
    18481 ttcactgggc aactatttta ccggtcctgg cagtattgcc cgatcctatc gtacggcgaa
    18541 aacgacgatg gtggtgggta aacagcggat gccagaaagt cgctgctatt tttatcagga
    18601 tctgatgtta cctgtgttac tcgacagttt gcgtggcgac tggcaggcca acgaactggc
    18661 gcgaccgctg gcgcggctga aagcgatgga caataacggc ttgctgcgac gaacgctggc
    18721 ggcgtggttt cgccacaatg tgcaaccgct ggcaacgtca aaggcgttgt ttattcatcg
    18781 taataccctg gagtatcggc ttaatcgtat atcggaactg accgggcttg atttgggcaa
    18841 ttttgatgac aggttgctgc tgtatgtggc gttacaactg gatgaagagc ggtagattat
    18901 gcgttaaggt tgaggcggcg ctggctcatt cccctcagcc cgaaggggag aggggaatgc
    18961 ccgagttaac cgtcaattcg ggattattta cgcgttagtt tttccagatc ggcttcaatc
    19021 tcgctgatct tattggttac gactgactcc aggtgacgta agtcgtcgag gatcttacgc
    19081 ttaagatcaa cttcgctgcg gtcacgctga cagatttgat caagctcatc aatgatatac
    19141 cgtagattcg ggctgatttc ctggacttct ttataaccct gacccacacc atcagcgacg
    19201 accgttttac gctgacgcgg atatttaaac ttaacgctct tggcgaaaaa ctcgcctttg
    19261 tctttctgga aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga
    19321 tcaatttctt caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct
    19381 caaaatgagt cagtaaattg tgcttatttt agcatttggc ttggcccgcc cggctcgact
    19441 gtgatcgcag aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta
    19501 cctgctttat tgtggcgata ttccatggga tggattagca catttatctt cgttttgcga
    19561 attacatgac agtccgaaac aagatgaaaa agtatttaaa aaagggggag gaagaatgag
    19621 taggacgaat tattctgagc cagaaagcat acgggcagca tcatgctgcc cgtatatgta
    19681 ctcttaatcg atggtacgca gcagttcgtt aatgccgact ttgccgcgag ttttcgcgtc
    19741 aactttctta acgataaccg cacagtagag gctgtatttg ccatcttttg acggcagatt
    19801 acctgaaaca accacagacc ccgccggaac gcgaccgtag tggatttcgc cggtttcacg
    19861 gtcgtaaata cgggtgctct gaccaatgta tacgcccata gagatgacgg aaccttcttc
    19921 gacaatcacc ccttcaacca cttcagagcg tgcgccaatg aagcagttat cttcaataat
    19981 ggtcgggtta gcctgcagcg gctccagcac gccgccgatg cccacgccac cggaaaggtg
    20041 gacgttttta ccaatctgcg cacaagaacc gacggtcgcc caggtatcaa ccatggtgcc
    20101 ttcatcaaca tatgcgccga tgttaacgta agacggcatc agcacggtgt tacgggcaat
    20161 aaacgcaccc tgacgtaccg ccgctggtgg cacaacgcgg aagccttctt tctggaaacg
    20221 tgcttcgtcg tagtcggcga atttcatcgg cactttgtcg aagtagcggc tttctgcccc
    20281 ttcgatcacc tgattatcat taatacggaa agagagcagc accgcttttt tcaaccactg
    20341 atgcgtcacc cactgaccgt caattttttc cgctacacgc agtgcgccgg aatccagcag
    20401 ggcgatcacc tgatttaccg cttcgcgggt aacggtgtct gcattggctg gcgtgatctc
    20461 ggcacggcgt tcaaaagcgg tttcaataat gttctgtaac tgctgcattg ttaaactctt
    20521 ttcatatcag taaacacatc accctttatc gtttggattg agggcctctg tcaaccgctg
    20581 atgcacttcc tgctgcaact cgttattaag cgcacgccgg tcagcggtgg caattatgaa
    20641 taaatcttct actcgctcgc caatggttgt aattcgggca ccatgaagcg aaattcccag
    20701 atcggcaaaa attttcccga ctcgcgccag cagtccaggt tggtcgaggg cgatcagttc
    20761 gaggaacgat ttgcggtcgg tatgggtcgg caaaaacgtt acttcggttt caacagtaaa
    20821 atggcgtaat ttggcgggtt ggcgacgggg ctgcggtggc tgccagctac tttgcgtcag
    20881 cacctgctcc agaccaaacc gaataacctc atggcgatct gcggacagcg ggctgccatc
    20941 gggttccagc acgataaagg tatccatcgc cataccgtcg cgagtggtga aaatttgtgc
    21001 gtcgtgaaca cttaaattgc ggcggtctaa ttcggcacag acggcggcaa acagataggg
    21061 gcggtccggg ctccagataa aaatctcggt gcctccacgc gtagcctgcg ggctaagcaa
    21121 taccagcggt ttgcttaaat catgctgtaa taaatggcgg gcatgccagg ccagttgatt
    21181 tgggctatgg cggacaaaat agttagcacg acagcgtgac caaatttggt gcagcgcctc
    21241 ttcgtcgatg ttatccatgc gcagtagtgc cagtgcctgg agttggtgat ggcgaacccg
    21301 ttcgcgcata tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg tggcaaaata
    21361 gagttcgcgc aacagacttt gcttccagct attccacagt gtttcgttgg tggcgcaaat
    21421 gtcagccact gtcaggcata ccagatagcg cagacgattt tccgtttgaa cttcttcggc
    21481 aaactgcttg atgacttcag ggtcctgaat atcgcggcgt tgtgctgtca ccgacatcag
    21541 caagtgctgg cgaaccagcc aggcaaccag ctgtgtttcg cgtgagttca gcccgtggag
    21601 ttctgcaaaa tgcactacat cctgagcacc gagaatggag tggtcgccgc cgcgtccttt
    21661 ggcgatatcg tgaaacagcg cggcgatgaa aatcagctca gttgacggca ggcgcggcca
    21721 gacgtccaca cacaacggat ggcgctggcg cgtttcttca ctggcaaaac tctccagttt
    21781 cagcatcacg cggatagtat gttcatccac cgtgtaggcg tggaacagat caaactgcat
    21841 ctgcccgacg atatgcgacc attgcggcat atacgcgccg agcacgctat ggcgatgcat
    21901 tggcaatagt ccgcgccgca ccgctccggg gtgacgcaga atgctcaaaa acagttttcg
    21961 tgcttgcgga atattacaca gcggttgttg cagatggcga cgggcatggc gtaactggcg
    22021 cagcgtggtg gagtaaatgc cggtgatcgc actgttgcgc accatggtgt agaacatacg
    22081 caagatggct tccggctggc gcataaatag tgtttcatca cgcaggtcga ttagcgtacc
    22141 gcgtagctga aactcatcgt cgagtggacg tggtttttcg tcggcgggaa gggcgaggat
    22201 ggcttcatcg aacagttgca gcagcatctg gttgagttca ctgacgcggc gtgtaacgcg
    22261 gaagtaatcc ttcatcatcc gctcgaccgg ttcgttgcct tcaccactgt aattcagacg
    22321 ctgagcgacg ctaagttggc gatcgaataa cagacgatta tcgtaacggc tgacgaccag
    22381 atgcagggca aagcgaatac gccacagtat atgcagacat tcgtttaatt ccgcccgctc
    22441 cgctgaggtt aagaagccaa acccgaccat ttcatccagc gatgttgcgc caaaatgacg
    22501 gcgggccacc cattgcagag tgtgaatatc gcgcaagccg ccagggctac ttttgatgtc
    22561 tggttcaagg ttgtagctgg tgccatggta acgctgatgg cgctggttct gttcttcaac
    22621 tttcgctgcg tagaacttgt cggaaggcca gaatccttcg ctaaaaatat gtttttgcag
    22681 ttcgaggaag agcgcaacat cgccaattaa taagcgggat tcgattaaat tggtggcgac
    22741 ggttaaatcc gataaccctt ccaacatgca ctcttcaagc gtgcgcacac tatgaccgac
    22801 ttccagcttt acatcccaga gcagcgttaa cagctcgccc actttttgcg cctgatcgtc
    22861 cgggagcttt ttacggctta aaatcagtaa atcgatgtca gaaagtggat gcagctcgcc
    22921 acgaccgtag ccaccgacgg caaccaatgc caggtcggca atctggctga atcccgcttc
    22981 aatccataat cgttgcagga gctggtctat gaactcggta cgtgcctcaa tcaactgttc
    23041 tgcagagatc ccattgtcaa aggcatcacc cagccaacgc tggaaagtat cgatatgggc
    23101 ttttatccca cagacggtta attcatcacg gggccaggcg catggatttt gcggttgacc
    23161 gggcagggtg gagagagcgg tgtttgcgta ctgttctgga agggtattca ttgtgcgcca
    23221 cccataagat taaattatcg cattaaaaaa gccggcattc gccggcttca tcttattcgt
    23281 cgtgcgagat tatcgccggg atggtgtcat ccttgcgtag cgtcaaaatt tcgcagccgt
    23341 tatcagtcac cacaatagta tgctcatatt gtgcagacaa gctgcgatct ttggttttta
    23401 ccgtccagcc atctttcatg gtgcggatct cttttttacc cgcgttgacc attggctcaa
    23461 tggtgaacgt catccccggt ttcagcacga cgttggtttc acgggagtca tagtgcagca
    23521 cctgcggttc ttcatggaag ccgcgaccaa taccgtgtcc gcaatattca cgaacgacgg
    23581 agaagccttc tgcttcgaca aatttctgaa tcgccgcacc gatttcgcgc agattaatgc
    23641 ctggttttac catgcgtagc gccaggtaca ggctttcttg cgtgatgcgg cacaggcgtt
    23701 cgcccatgat ggtcggctta ccgacgataa acattttcga ggtatcgccg tggaaaccat
    23761 ctttgattac ggtgacatca atgttaacga tatcgccatc tttcagcagc ttagcatcgt
    23821 ccgggatacc gtggcacacc acttcattaa tagagatgca aacggatttc ggatagccgt
    23881 gatagccgag gcaggcagaa accgcgtgtt gttcattaac aatgtaatca ttacagatgc
    23941 gatccagctc gccggtgctg acgcccggtt taacatacgg ttcgatcatc tccagcactt
    24001 cggcagccag tcggccagcg acgcgcattt tttcgatatc ttctggggtc ttgattgaga
    24061 tagccattaa ttctgtccat cagcgtcggt gataccgaca atatatatgt aagtgccgtc
    24121 aatggtatca cacccgggca aattgagaat cattctgaat ttcgccaaac gtgccactga
    24181 aggttttcta taatagaaaa ttcgacgtct gatgctgtac acagcgccaa caattattgg
    24241 tgtccacgac gtatttgtgg tataaagcgc gccggacttc cgatccattt cgtatacaca
    24301 gactggacgg aagcgacaat ctcactttgt gtaacaacac acacgtatcg gcacatattc
    24361 cggggtgccc tttggggtcg gtaatatggg atacgtggag gcataacccc aacttttata
    24421 tagaggtttt aatcatggca actgtttcca tgcgcgacat gctcaaggct ggtgttcact
    24481 tcggtcacca gacccgttac tggaacccga aaatgaagcc gttcatcttc ggtgcgcgta
    24541 acaaagttca catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa gctctggctg
    24601 aactgaacaa gattgtttct cgcaaaggta aaatcctttt cgttggtact aaacgcgctg
    24661 caagcgaagc ggtgaaagac gctgctctga gctgcgacca gttcttcgtg aaccatcgct
    24721 ggctgggcgg tatgctgact aactggaaaa ccgttcgtca gtccatcaaa cgtctgaaag
    24781 acctggaaac tcagtctcag gacggtactt tcgacaagct gaccaagaaa gaagcgctga
    24841 tgcgcactcg tgagctggag aaactggaaa acagcctggg cggtatcaaa gacatgggcg
    24901 gtctgccgga cgctctgttt gtaatcgatg ctgaccacga acacattgct atcaaagaag
    24961 caaacaactt gggtattccg gtatttgcta tcgttgatac caactctgat ccggacggtg
    25021 ttgacttcgt tatcccgggt aacgacgacg caatccgtgc tgtgaccctg tacctgggcg
    25081 ctgttgctgc aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag gcggaagaaa
    25141 gcttcgtaga agctgagtaa taaggcttga taactccccc aaaatagttc gagttgcaga
    25201 aaggcggcaa gctcgagaat tcccgggagc ttacatcagt aagtgaccgg gatgagcgag
    25261 cgaagacaac gcatctgcgg cgcgaaatat gaagggggag agcccttata gaccaggtag
    25321 tacacgtttg gttagggggc ctgcatatgg cccccttttt cacttttata tctgtgcggt
    25381 ttaatgccgg gcagatcaca tctccgagga ttttagaatg gctgaaatta ccgcatccct
    25441 ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa aagcactgac
    25501 tgaagctaac ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg gtgctattaa
    25561 agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg atcaaaacca aaatcgacgg
    25621 caactacggc atcattctgg aagttaactg ccagactgac ttcgttgcaa aagacgctgg
    25681 tttccaggcg ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa tcactgacgt
    25741 tgaagttctg aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga aaattggtga
    25801 aaacatcaac attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt cttatcagca
    25861 cggtgcgcgt atcggcgttc tggttgctgc taaaggcgct gacgaagagc tggttaaaca
    25921 catcgctatg cacgttgctg caagcaagcc agaattcatc aaaccagaag acgtatccgc
    25981 tgaagtggta gaaaaagaat accaggtaca gctggatatc gcgatgcagt ctggtaagcc
    26041 gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg gcgaagtttc
    26101 tctgaccggt cagccgttcg ttatggaacc aagcaaaact gttggtcagc tgctgaaaga
    26161 gcataacgct gaagtgactg gcttcatccg tttcgaagtg ggtgaaggca tcgagaaagt
    26221 tgagactgac tttgcagcag aagttgctgc gatgtccaag cagtcttaat tatcaaaaag
    26281 gagccgcctg agggcggctt ctttttgtgc ccatcttgta aattcagcta acccttgtgg
    26341 ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa tcgttgacag
    26401 tctcaggaaa gaaacatggc taccaatgca aaacccgtct ataaacgcat tctgcttaag
    26461 ttgagtggcg aagctctgca gggcactgaa ggcttcggta ttgatgcaag catactggat
    26521 cgtatggctc aggaaatcaa agaactggtt gaactgggta ttcaggttgg tgtggtgatt
    26581 ggtgggggta acctgttccg tggcgctggt ctggcgaaag cgggtatgaa ccgcgttgtg
    26641 ggcgaccaca tggggatgct ggcgaccgta atgaacggct tggcaatgcg tgatgcactg
    26701 caccgcgcct atgtgaacgc tcgtctgatg tccgctattc cattgaatgg cgtgtgcgac
    26761 agctacagct gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt gatcctctcc
    26821 gccggtacag gtaacccgtt ctttaccacc gactcagcag cttgcctgcg tggtatcgaa
    26881 attgaagccg atgtggtgct gaaagcaacc aaagttgacg gcgtgtttac cgctgatccg
    26941 gcgaaagatc caaccgcaac catgtacgag caactgactt acagcgaagt gctggaaaaa
    27001 gagctgaaag tcatggacct ggcggccttc acgctggctc gtgaccataa attaccgatt
    27061 cgtgttttca atatgaacaa accgggtgcg ctgcgccgtg tggtaatggg tgaaaaagaa
    27121 gggactttaa tcacggaata attcccgtga tggataaata agggtaagat tccgcgtaag
    27181 tatcgcgggg gcgtaagtct ggttataagg cgttattgtt gcaggcagtt tggtcacggc
    27241 cagcgcgcag caaccggagc gtacaaaagt acgtgaggat ggcgagcact gctcggggcc
    27301 aaaatggcaa ataaaatagc ctaataatcc agacgattac ccgtaatatg tttaatcagg
    27361 gctatactta gcacacttcc actgtgtgtg actgtctggt ctgactgaga caagttttca
    27421 aggattcgta acgtgattag cgatatcaga aaagatgctg aagtacgcat ggacaaatgc
    27481 gtagaagcgt tcaaaaccca aatcagcaaa atacgcacgg gtcgtgcttc tcccagcctg
    27541 ctggatggca ttgtcgtgga atattacggc acgccgacgc cgctgcgtca gctggcaagc
    27601 gtaacggtag aagattcccg tactctgaaa atcaacgtgt ttgatcgttc aatgtctccg
    27661 gccgttgaaa aagcgattat ggcctccgat ctcggcctga acccgaactc tgcgggtagc
    27721 gacatccgtg ttccgctgcc gccgctgacg gaagaacgtc gtaaagatct gaccaaaatc
    27781 gttcgtggtg aagcagaaca agcgcgtgtt gcagtacgta atgtgcgtcg tgacgcgaac
    27841 gacaaagtga aagcactgtt gaaagataaa gagatcagcg aagacgacga tcgccgttct
    27901 caggacgatg tacagaaact gactgatgct gcaatcaaga aaattgaagc ggcgctggca
    27961 gacaaagaag cagaactgat gcagttctga tttcttgaac gacaaaaacg ccgctcagta
    28021 gatccttgcg gatcggctgg cggcgttttg ctttttattc tgtctcaact ctggatgttt
    28081 catgaagcaa ctcaccattc tgggctcgac cggctcgatt ggttgcagca cgctggacgt
    28141 ggtgcgccat aatcccgaac acttccgcgt agttgcgctg gtggcaggca aaaatgtcac
    28201 tcgcatggta gaacagtgcc tggaattctc tccccgctat gccgtaatgg acgatgaagc
    28261 gagtgcgaaa cttcttaaaa cgatgctaca gcaacagggg agccgcaccg aagtcttaag
    28321 tgggcaacaa gccgcttgcg atatggcagc gcttgaggag gttgatcagg tgatggcagc
    28381 cattgttggt gctgctgggc tgttacctac gcttgctgcg atccgcgcgg gtaaaaccat
    28441 tttgctggcc aataaagaat cactggttac ctgcggacgt ctgtttatgg acgccgtaaa
    28501 gcagagcaaa gcgcaattgt taccggtcga tagcgaacat aacgctattt ttcagagttt
    28561 accgcaacct atccagcata atctgggata cgctgacctt gagcaaaatg gcgtggtgtc
    28621 cattttactt accgggtctg gtggcccttt ccgtgagacg ccattgcgcg atttggcaac
    28681 aatgacgccc gatcaagctt gccgtcatcc gaactggtcg atgggacgta aaatttctgt
    28741 cgattcggct accatgatga acaaaggtct ggaatacatt gaagcgcgtt ggctgtttaa
    28801 cgccagcgcc agccagatgg aagtgctgat tcacccgcag tcagtgattc actcaatggt
    28861 gcgctatcag gacggcagtg ttctggcgca gctgggggaa ccggatatgc gtacgccaat
    28921 tgcccacacc atggcatggc cgaatcgcgt gaactctggc gtgaagccgc tcgatttttg
    28981 caaactaagt gcgttgacat ttgccgcacc ggattatgag cgttatccat gcctgaaact
    29041 ggcgatggag gcgttcgaac aaggccaggc agcgacgaca gcattgaatg ccgcaaacga
    29101 aatcaccgtt gctgcttttc ttgcgcaaca aatccgcttt acggatatcg ctgcgttgaa
    29161 tttatccgta ctggaaaaaa tggatatgcg cgaaccacaa tgtgtggacg atgtgttatc
    29221 tgttgatgcg aacgcgagtg aagtcgccag aaaagaggtg atgcgtctcg caagctgagg
    29281 ataatccggc tacagagagt cgcgctattt gttagcgtag ggcttcagtg atatagtctg
    29341 cgccatctga tcgtaagtag ttggctttat aaggtcaggt atgccgtggt tttacacggc
    29401 ttttttttgt ataggcttca gtattcctga gtaccgtaaa ccctgtcagg gaataaaaaa
    29461 cgcgtgatgt tgtctgctac tcaaccactt agcgaaaaat tgccagcgca tggctgccgt
    29521 catgttgcga tcattatgga cggcaatggt cgctgggcaa aaaagcaagg gaagattcgt
    29581 gcctttgggc ataaagccgg ggcaaaatcc gtccgccggg ctgtctcttt tgcggccaac
    29641 aacggtattg aggcgttaac gctgtatgcc tttagtagtg aaaactggaa ccgaccagcg
    29701 caggaagtca gtgcgttaat ggaactgttt gtgtgggcgc tcgatagcga agtaaaaagt
    29761 ctgcaccgac ataacgtgcg tctgcgtatt attggcgata ccagtcgttt taactcgcgt
    29821 ttgcaagaac gtattcgtaa atctgaagcg ctaacagccg gaaataccgg tctgacgctg
    29881 aatattgcgg cgaactacgg tggacgttgg gatatagtcc agggagtcag gcaactggct
    29941 gaaaaggtgc agcaaggaaa cctgcaacca gatcagatag atgaagagat gttaaaccag
    30001 catgtctgta tgcatgaact ggcccctgta gatttagtaa ttaggactgg gggagagcat
    30061 cgcattagta actttttgct ttggcaaatt gcctatgccg aactttactt tacagatgtt
    30121 ctctggcccg atttcgatga acaagacttt gaaggggcgt taaatgcctt tgctaatcga
    30181 gagcgtcgtt tcggcggcac cgagcccggt gatgaaacag cctgatgggg gtcgcttttg
    30241 ctgaagtatc gcctgatatc tgcttttgtg ttaatacccg tcgtcatcgc ggcgttgttt
    30301 ctgttgccgc cggtggggtt cgccattgta acgctggtgg tctgcatgct ggcagcgtgg
    30361 gaatggggac agcttagcgg ttttaccact cgttcgcagc gagtatggtt ggcggtgtta
    30421 tgcgggttat tgttggcgct gatgcttttt ctgttgccgg aatatcaccg aaatattcat
    30481 caaccgctgg ttgaaatctc actttgggct tcgttgggtt ggtggattgt cgcgctattg
    30541 ctggtgctgt tttacccagg ttccgcagca atctggcgta actctaaaac attgcgcctt
    30601 atttttggcg tgctaaccat tgttcccttc ttctggggca tgctggcgtt acgggcctgg
    30661 cactatgacg agaatcatta cagtggcgca atatggctgc tctatgtcat gatcctggta
    30721 tggggcgctg actccggcgc atatatgttt ggcaaattgt ttggtaaaca taagctggca
    30781 ccgaaggttt ctccgggtaa aacctggcaa ggctttatag gtggactcgc tactgcagcg
    30841 gtaatctcat ggggttatgg catgtgggcg aatctcgacg tcgctcccgt caccttactc
    30901 atttgctcta ttgtcgcagc gttagcctca gtgctcggcg atctgaccga gagtatgttt
    30961 aagcgtgaag caggaattaa ggacagcggt catttaattc caggacacgg tggtatttta
    31021 gatcgtattg atagcctgac ggctgcggta ccggtctttg cttgcttgtt gttactggta
    31081 ttcaggacgc tttaacggaa ggtaatatgc tgagttttct ctgggatttg gcttcgttca
    31141 tcgttgcact gggtgtactt atcaccgtgc atgaatttgg tcatttctgg gttgcccggc
    31201 gttgtggtgt tcgcgttgag cgtttctcaa tagggtttgg taaggcgctc tggcggcgaa
    31261 ctgataagct cggcaccgaa tatgttatcg ccctgatccc gttgggcggt tatgtcaaaa
    31321 tgctggatga gcgcgcagaa ccggtcgttc cggaactccg ccaccatgcc ttcaataata
    31381 aatctgtcgg ccaacgagcg gcgattattg ccgcaggtcc ggttgcaaac ttcatttttg
    31441 ctatctttgc ctactggctg gtttttatta ttggtgtgcc tggcgtacgt ccggtggttg
    31501 gcgaaatagc agccaattcg atagctgcgg aagcacaaat tgcaccaggt acggaactaa
    31561 aagccgtaga tggtatcgaa acgcctgatt gggatgccgt gcgtttgcag ttggtcgata
    31621 aaattggcga tgaaagcacc accattacag tagcgccatt tgacagcgac caacggcggg
    31681 atgtaaagct cgatttacgt cactgggcgt ttgagcctga taaagaagat ccggtatctt
    31741 cgctggggat tcgtcctcgt gggccgcaaa ttgaacctgt actggaaaat gtgcagccaa
    31801 actcggcggc aagcaaggca ggtttgcaag caggcgacag gatcgttaaa gtcgatggtc
    31861 agcccttaac gcagtgggtg acctttgtga tgcttgtccg ggataacccg ggtaaatcct
    31921 tagcgttaga aatcgaaagg caggggagtc ccttgtcttt gacattaatc ccggagagta
    31981 aaccgggtaa tggtaaagcg attggttttg tcggtattga gccgaaagtc attcctttgc
    32041 cagatgagta taaagttgta cgccagtatg ggccgttcaa cgccatcgtc gaagccacgg
    32101 acaaaacgtg gcagctgatg aagctgacgg tcagtatgct gggaaaattg atcaccggtg
    32161 atgtgaaact gaacaacctc agtgggccga tctctatcgc caagggggct gggatgacag
    32221 cggaactcgg ggtagtttat tacctgccgt ttcttgcgct tattagcgtg aacttaggga
    32281 taattaacct gtttccgttg cccgtacttg acggggggca tctgctgttc cttgcgatcg
    32341 aaaagatcaa gggcggaccg gtatccgagc gggttcaaga cttttgttat cgcattggct
    32401 cgattctgct ggtgctgtta atggggcttg cacttttcaa tgatttctct cggttatgag
    32461 agttagttag gaagaacgca taataacgat ggcgatgaaa aagttgctca tagcgtcgct
    32521 gctgtttagc agcgccaccg tatacggtgc tgaagggttc gtagtgaaag atattcattt
    32581 cgaaggcctt cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg tgcgtacagg
    32641 cgacacggtt aatgatgaag atatcagtaa taccattcgc gctctgtttg ctaccggcaa
    32701 ctttgaggat gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg taaaagaacg
    32761 tccgaccatt gccagcatta ctttctccgg taacaaatcg gtgaaagatg acatgctgaa
    32821 gcaaaacctc gaggcttctg gtgtgcgtgt gggcgaatcc ctcgatcgca ccaccattgc
    32881 cgatatcgag aaaggtctgg aagacttcta ctacagcgtc ggtaaatata gcgccagcgt
    32941 taaagctgtc gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg tgttccagga
    33001 aggtgtgtca gctgaaatcc agcaaattaa cattgttggt aaccatgctt tcaccaccga
    33061 cgaactgatc tctcatttcc aactgcgtga cgaagtgccg tggtggaatg tggtaggcga
    33121 tcgtaaatac cagaaacaga aactggcggg cgatcttgaa accctgcgca gctactatct
    33181 ggaccgcggt tatgcccgtt tcaacatcga ctctacccag gtcagtctga cgccagataa
    33241 aaaaggtatt tacgtcacgg tgaacatcac cgaaggcgat cagtacaagc tttctggcgt
    33301 tgaagtgagc ggcaaccttg ccgggcactc cgctgaaatt gagcaactga ctaagatcga
    33361 gccgggcgag ctgtataacg gcactaaagt gaccaaaatg gaagatgaca ttaaaaagct
    33421 tctcggtcgc tatggttatg cctatccgcg cgtacagtcg atgcctgaaa ttaacgatgc
    33481 cgacaaaacc gttaaattac gcgtgaacgt tgatgcgggt aaccgtttct acgtgcgtaa
    33541 gatccgtttt gaaggtaacg atacctcgaa agatgccgtc ctgcgtcgcg aaatgcgtca
    33601 gatggaaggt gcatggttgg ggagcgatct ggtcgatcag ggtaaggagc gtctgaatcg
    33661 tctgggcttc tttgaaactg tcgataccga tacccaacgt gttccgggta gcccggacca
    33721 ggttgatgta gtctacaagg tgaaagaacg taacactggt agcttcaact tcgggattgg
    33781 ttacggtact gaaagtggcg tgagcttcca ggctggcgtg caacaggata actggttagg
    33841 tacaggttat gctgttggta tcaacgggac caaaaacgat taccagacct atgctgaact
    33901 gtcggtaacc aacccgtact tcaccgtaga tggcgtaagc ctcggtggtc gtctcttcta
    33961 taatgacttc caggcagatg acgccgacct gtccgactat accaacaaga gttatggtac
    34021 agacgtgacg ttgggcttcc cgattaacga atataactcg ctgcgtgcag gtctgggtta
    34081 tgtacataac tccctgtcca acatgcagcc tcaggttgcg atgtggcgtt atctgtactc
    34141 tatgggtgaa catccgagca cctctgatca ggataacagc ttcaaaacgg acgacttcac
    34201 gttcaactat ggttggacct ataacaagct tgaccgtggt tacttcccga cagatggttc
    34261 acgtgtcaac ctgaccggta aagtgaccat tcctggatcg gataacgaat actacaaagt
    34321 gacgttagac acggcgactt atgtgccgat cgatgacgat cacaaatggg ttgttctggg
    34381 tcgtacccgc tggggttatg gtgatggttt aggcggcaaa gagatgccgt tctacgagaa
    34441 cttctatgcc ggtggttcca gcaccgtgcg tggcttccag tccaatacca ttggtccgaa
    34501 agcagtttac ttcccgcatc aggccagtaa ttatgatccg gactatgatt acgaatgtgc
    34561 gactcaggac ggcgcgaaag acctgtgtaa atcggatgat gctgtaggcg gtaacgccat
    34621 ggcggttgcc agcctcgagt tcatcacccc gacgccgttt attagcgata agtatgctaa
    34681 ctcggttcgt acttcattct tctgggatat gggtaccgtt tgggatacaa actgggattc
    34741 cagccaatat tctggatatc cggactatag tgatccaagc aatatccgta tgtctgcggg
    34801 tatcgcatta caatggatgt ccccattggg gccgttggtg ttctcctacg cccagccgtt
    34861 caaaaagtac gatggagaca aggcagaaca gttccagttt aacatcggta aaacctggta
    34921 agtgttctcc acaaaggaat gtagtggtag tgtagcgatg actttaggcg atcaatataa
    34981 gatcgccggg ccacgcaaag aactgcaccc tccggtgcaa atgggatggt aaggagttta
    35041 ttgtgaaaaa gtggttatta gctgcaggtc ttggtttagc actggcaact tctgctcagg
    35101 cggctgacaa aattgcaatc gtcaatatgg gcagcctgtt ccagcaggta gcgcagaaaa
    35161 ccggtgtttc taacacgctg gaaaatgagt tcaaaggccg tgccagcgaa ctgcagcgta
    35221 tggaaaccga tctgcaggct aaaatgaaaa aactgcagtc catgaaagcg ggcagcgatc
    35281 gcaccaaact ggaaaaagac gtgatggctc agcgccagac ttttgctcag aaagcgcagg
    35341 cttttgagca ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg gttactcgta
    35401 tccagactgc tgtgaaatcc gttgccaaca gccaggatat cgatctggtt gttgatgcaa
    35461 acgccgttgc ttacaacagc agcgatgtaa aagacatcac tgccgacgta ctgaaacagg
    35521 ttaaataagt aatgccttca attcgactgg ctgatttagc gcagcagttg gatgcagaac
    35581 tacacggtga tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct gcacaaacag
    35641 gtcacattac gttcatggtt aacccaaaat accgtgagca tttaggcttg tgccaggcgt
    35701 ccgcggttgt catgacccag gacgatcttc ctttcgcgaa aagtgccgcg ctggtagtga
    35761 agaatcccta cctgacttac gcgcgcatgg cgcaaatttt agataccacg ccgcagcccg
    35821 cgcagaacat tgcacccagt gcggtgatcg acgcgacggc gaagctgggt aacaacgtat
    35881 cgattggcgc taacgcggtg attgagtccg gcgttgaact gggcgataac gtgattatcg
    35941 gtgccggttg cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt ctctgggcga
    36001 acgtaaccat ttaccatgag atccagatcg gtcagaattg cctgatccag tccggaacag
    36061 tggtaggcgc agacggcttt ggttatgcca acgatcgtgg taactgggtg aagatcccac
    36121 agattggtcg cgtaattatt ggcgatcgcg tggagatcgg tgcctgcaca accatcgatc
    36181 gcggcgcgct ggatgacact gttattggca atggcgtgat cattgataac cagtgccaga
    36241 ttgcacataa cgtcgtgatt ggcgacaata cggcggttgc cggtggcgtc attatggcgg
    36301 gcagcctgaa aattggtcgt tactgcatga tcggcggagc cagcgtaatc aacgggcata
    36361 tggaaatatg cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt cccatcactg
    36421 aaccaggcgt ctattcctca ggcattccgc tgcaacccaa caaagtctgg cgcaaaaccg
    36481 ctgcactggt gatgaacatt gatgacatga gcaagcgtct gaaatcgctt gagcgcaagg
    36541 ttaatcaaca agactaacgt tccatctttt gttcgccaaa ctttacggcc tgtctcattc
    36601 ttacgattgc ggcaggccgt gttattattg tcgtttctta tattttgaca ggaagagtat
    36661 cttgactact aacactcata ctctgcagat tgaagagatt ttagaacttc tgccgcaccg
    36721 tttcccgttc ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt ttctgcgcgc
    36781 agtaaaaaat gtctctgtca atgagccatt cttccagggc catttccctg gaaaaccgat
    36841 tttcccgggt gtgctgattc tggaagcaat ggcacaggca acaggtattc tggcgtttaa
    36901 aagcgtagga aaactggaac cgggtgagct gtactacttc gctggtattg acgaagcgcg
    36961 cttcaagcgc ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt tcgaaaaaac
    37021 gcgccgcggc ctgacccgtt ttaaaggggt tgctctggtc gatggtaaag tagtttgcga
    37081 agcaacgatg atgtgtgctc gtagccggga ggcctgatac gtgattgata aatccgcctt
    37141 tgtgcatcca accgccattg tggaagaggg cgcgtcaatt ggcgcgaacg cacacattgg
    37201 tcctttttgt atcgttggac cccatgtcga aattggtgag ggtaccgtac tgaaatctca
    37261 cgttgtcgtg aatggtcata ctaaaattgg ccgcgataat gagatttatc agtttgcctc
    37321 catcggcgaa gttaaccagg atttgaaata tgctggcgaa ccgacccgtg tggaaatcgg
    37381 cgatcgtaac cgcattcgcg aaagcgtcac cattcatcgt ggcacagtcc agggcggtgg
    37441 attgacgaag gtgggcagcg acaacttact gatgatcaac gcgcacattg cgcacgattg
    37501 tacggtaggt aaccgctgta ttctcgccaa caacgcaacg ctggcgggtc acgtatcggt
    37561 tgacgacttc gcgatcatcg gcggcatgac cgcagtccat cagttctgca tcattggtgc
    37621 gcatgtgatg gttggcggct gctccggtgt ggcgcaggac gtccctcctt atgttattgc
    37681 gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc gccgcggatt
    37741 cagccgtgag gcgattaccg ctatccgcaa tgcgtataag ctaatttatc gtagcggtaa
    37801 aacgctcgat gaagtgaaac cggaaattgc tgaactggcg gaaacatatc cggaagtgaa
    37861 agcctttacc gatttctttg cacgctcaac gcgcggtctg attcgttaat gactgaccag
    37921 cgtccattaa cgattgccct ggtcgccgga gaaacctccg gcgatatcct gggggccggt
    37981 ttaatccgcg ctctgaaaga acgtgtgccc aacgcccgct ttgttggtgt tgccgggcca
    38041 cgaatgcagg ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc ggtgatgggc
    38101 attgttgaag tgctcggtcg tctgcgtcgc ttactgcata ttcgtgccga tctgacaaag
    38161 cgttttggcg aactgaagcc agatgttttt gttggtattg atgcgcccga cttcaatatt
    38221 actcttgaag gtaacctcaa aaagcagggt atcaaaacca ttcattacgt cagtccatcc
    38281 gtctgggcgt ggcgacagaa acgcgttttc aaaataggca gagccaccga tctggtgctc
    38341 gcatttctgc ctttcgaaaa agcgttttat gacaaataca atgtaccgtg ccgctttatc
    38401 ggtcatacca tggctgatgc catgccgtta gatccagata aaaatggtgc ccgtgatgtg
    38461 ctggggatcc cttacgatgc ccactgtctg gcattgttgc cgggcagccg tggtgcagaa
    38521 gtcgaaatgc ttagtgccga tttcttgaaa actgcccagc ttttgcgcca gacatatccg
    38581 gatctcgaaa tcgtggtgcc gctggtgaat gccaaacgcc gcgagcagtt tgaacgcatc
    38641 aaagctgaag tcgcgccaga cctgtcggtt catttgctgg atggaatggg ccgtgaggcg
    38701 atggtcgcca gcgatgcggc actactggcg tcggggacgg cagccctgga gtgtatgctg
    38761 gcgaaatgcc cgatggtggt gggatatcgc atgaagcctt ttaccttctg gttggcgaag
    38821 cggctggtga aaactgatta tgtctcgctg ccaaatctgc tggcgggcag agagttagtc
    38881 aaagagttat tgcaggaaga gtgtgagccg caaaaactgg ctgcggcgct gttaccgctg
    38941 ttggcgaacg ggaaaaccag ccacgcgatg cacgatacct tccgtgaact gcatcagcag
    39001 atccgctgca atgccgatga gcaggcggca caagccgttc tggagttagc acaatgatcg
    39061 aatttgttta tccgcacacg cagctggttg cgggtgtgga tgaagtcgga cgcgggccgt
    39121 tagttggcgc ggtcgtcacc gctgcggtga tccttgaccc ggcgcgcccg attgccgggc
    39181 tgaatgattc caaaaagctg agcgaaaaac gccgtctggc gctctatgaa gagatcaaag
    39241 agaaagcgtt gagctggagt ctgggccgcg cggaacccca cgaaatcgac gagctgaaca
    39301 ttcttcatgc gaccatgctg gcgatgcagc gtgccgtcgc tgggctgcat attgcgccgg
    39361 aatatgtgtt gattgatggt aaccgctgcc cgaaattacc gatgcctgcg atggctgtgg
    39421 tgaaagggga tagccgcgta ccagaaatca gtgccgcgtc tatcctggcg aaagtgacgc
    39481 gtgacgccga aatggcggcg ctggatattg ttttcccgca atatggtttt gcccaacata
    39541 aagggtatcc aaccgctttt catctggaaa aactggctga atatggcgcg actgaacatc
    39601 atcgtcgcag ctttgggcct gtcaaacgcg cactgggact tgcgtcctga ttcttgtgtc
    39661 gagattaagt aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac ctgcgggtgc
    39721 acagcgacta ctcgatgatc gatggcttgg ccaaaaccgc accgctggta aaaaaggcgg
    39781 cggcgttggg tatgccagca ctggcgatca ccgatttcac caacctttgc ggtctggtga
    39841 agttctacgg agcgggacat ggcgcaggga ttaagcctat cgtcggggca gattttaatg
    39901 tccagtgcga cctgctgggt gatgagttaa cccacctgac ggtactggcg gcgaacaata
    39961 ccggctatca gaatctggcg ttgctgatct caaaagcgta tcagcgcggg tacggtgccg
    40021 ccgggccgat catcgatcgc gactggctta tcgaattaaa cgaagggttg atccttcttt
    40081 ccggcgggcg catgggcgac gtcggacgca gtcttttgcg tggtaacagc gcgctggtag
    40141 atgagtgtgt cgcgttttat gaagaacact tcccggatcg ctattttctc gagctgatcc
    40201 gcaccggcag gccggatgaa gaaagctatc tgcacgcggc ggtggaactg gcggaagcgc
    40261 gcggtttgcc cgtcgtggcg actaacgatg tgcgctttat cgacagcagc gactttgacg
    40321 cacacgaaat ccgcgtcgcg atccacgacg gctttaccct cgacgatcct aaacgcccgc
    40381 gtaactattc gccgcagcaa tatatgcgta gcgaagagga gatgtgtgag ctgtttgccg
    40441 acatcccaga agcccttgcc aacaccgttg agatcgccaa acgctgtaac gtaactgtgc
    40501 gtcttggtga atacttcctg ccgcagttcc cgaccgggga catgagcacc gaagattatc
    40561 tggtcaagcg tgcaaaagaa ggcctggaag agcgtctggc atttttattc cctgacgagg
    40621 aagaacgtct taaacgccgc ccggaatatg acgaacgtct ggagactgaa cttcaggtta
    40681 tcaaccagat gggcttcccg ggctacttcc tcatcgttat ggaatttatc cagtggtcga
    40741 aagataacgg cgtaccggta gggccaggcc gtggctccgg tgcgggttca ctggtggcct
    40801 acgcgctgaa aatcaccgac ctcgatccgc tggaatttga cctgctgttc gaacgtttcc
    40861 ttaacccgga acgtgtctcc atgcctgact tcgacgttga cttctgtatg gagaaacgcg
    40921 atcaggttat cgaacacgtg gcggacatgt acggtcgtga tgcggtatcg cagattatca
    40981 ccttcggtac gatggcggcg aaagcggtga tccgcgacgt aggccgcgtg ctggggcatc
    41041 cgtacggctt tgtcgaccgt atctcgaaac tgatcccgcc cgatccgggg atgacgctgg
    41101 cgaaagcgtt tgaagccgag ccgcagctgc cggaaatcta cgaagcggat gaagaagtta
    41161 aggcgctgat cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc ggtaagcacg
    41221 ccggtggggt ggttatcgcg ccgaccaaaa ttaccgattt tgcgccgctt tattgcgatg
    41281 aagagggcaa acatccggtc acccagtttg ataaaagcga cgttgaatac gccggactgg
    41341 tgaagttcga cttccttggt ttgcgtacgc tcaccatcat caactgggcg ctggagatga
    41401 tcaacaagcg gcgggcgaag aatggcgagc caccgctgga tatcgccgcg atcccgctgg
    41461 atgacaagaa aagcttcgac atgctgcaac gctcggaaac cacggcggta ttccagcttg
    41521 aatcgcgcgg catgaaggac ctgatcaagc gtctgcaacc tgactgcttc gaagatatga
    41581 tcgcactggt ggcactgttc cgccctggtc cgttgcagtc agggatggtg gataacttta
    41641 tcgaccgtaa acatggtcgt gaagagatct cctatccgga cgtgcagtgg cagcatgaaa
    41701 gcctgaaatc ggtactggag ccaacctacg gcattatctt gtatcaggaa caggtcatgc
    41761 agattgccca ggtgctttct ggttataccc tcggtggcgc ggatatgctg cgccgtgcga
    41821 tgggtaagaa aaagccggaa gagatggcta agcagcgttc tgtatttgct gaaggtgcag
    41881 aaaagaacgg aatcaacgcc gaactggcga tgaaaatctt cgacctggtg gagaaattcg
    41941 cgggttacgg atttaacaaa tcgcactctg cggcctatgc tttggtgtca tatcaaacgt
    42001 tatggctgaa agcgcactat ccggcggagt ttatggcggc ggtaatgacc gccgatatgg
    42061 acaacaccga gaaggtggtg ggcctggtgg atgagtgctg gcggatgggg ctgaaaatcc
    42121 tgccaccaga tataaactcc ggtctttacc atttccacgt caacgacgat ggcgaaatcg
    42181 tgtatggtat tggcgccatc aaaggggtag gtgaaggtcc gattgaggcc atcatcgaag
    42241 cccgtaataa aggcggctac ttccgcgaac tgtttgatct ctgcgcccgt accgacacca
    42301 aaaagttaaa ccggcgggtg ctggaaaaac tgatcatgtc cggggcgttt gaccgtcttg
    42361 ggccacaccg cgcggcgctg atgaactcgc tgggtgatgc gttaaaagcg gcagatcaac
    42421 acgcgaaagc ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc gaagagccgg
    42481 aacaaattga acaatcctac gccagctgcc aaccgtggcc ggagcaggtg gtattagatg
    42541 gggaacgtga aacgttaggc ctgtacctga ccggacaccc tatcaaccag tatttaaaag
    42601 agattgagcg ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca gaacgtggta
    42661 aagtcatcac ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc aagcgcggca
    42721 atcgtatcgg tatctgcacg ctggatgacc gttccgggcg gctggaagtg atgttgttta
    42781 ctgacgccct ggataaatac cagcaattgc tggaaaaaga ccgcatactt atcgtcagcg
    42841 gacaggtcag ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc gaagtgatgg
    42901 atattgacga agcccgggaa aaatatgctc gcgggcttgc tatctcgctg acggacaggc
    42961 aaattgatga ccagctttta aaccgactcc gtcagtctct ggaaccccac cgctctggga
    43021 caattccagt acatctctac tatcagaggg cggatgcacg cgcgcggttg cgttttggcg
    43081 cgacgtggcg tgtctctccg agcgatcgtt tattaaacga tctccgtggc ctcattggtt
    43141 cggagcaggt ggaactggag tttgactaat acaggaatac tatgagtctg aatttccttg
    43201 attttgaaca gccgattgca gagctggaag cgaaaatcga ttctctgact gcggttagcc
    43261 gtcaggatga gaaactggat attaacatcg atgaagaagt gcatcgtctg cgtgaaaaaa
    43321 gcgtagaact gacgcgtaaa atcttcgccg atctcggtgc atggcagatt gcgcaactgg
    43381 cacgccatcc gcagcgtcct tataccctgg attacgttcg cctggcattt gatgaatttg
    43441 acgaactggc tggcgaccgt gcgtatgcag acgataaagc tatcgtcggt ggtatcgccc
    43501 gtctcgatgg tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa accaaagaaa
    43561 aaattcgccg taactttggt atgccagcgc cagaaggtta ccgcaaagca ctgcgtctga
    43621 tgcaaatggc tgaacgcttt aagatgccta tcatcacctt tatcgacacc ccgggggctt
    43681 atcctggcgt gggcgcagaa gagcgtggtc agtctgaagc cattgcacgc aacctgcgtg
    43741 aaatgtctcg ccttggcgta ccggtagttt gtacggttat cggtgaaggt ggttctggcg
    43801 gtgcgctggc gattggcgtg ggcgataaag tgaatatgct gcaatacagc acctattccg
    43861 ttatctcgcc ggaaggttgt gcgtccattc tgtggaagag cgccgacaaa gcgccgctgg
    43921 cggctgaagc gatgggtatc atcgctccgc gtctgaaaga gctgaaactg atcgactcca
    43981 tcatcccgga accgctgggc ggcgctcacc gtaacccgga agcgatggcg gcatcgttga
    44041 aagcgcaact gctggcggat ctggccgatc tcgatgtgtt aagcactgaa gatttaaaaa
    44101 atcgtcgtta tcagcgcctg atgagctacg gttacgcgta attcgcaaaa gttctgaaaa
    44161 agggtcactt cggtggccct tttttatcgc cacggtttga gcaggctatg attaaggaag
    44221 gattttccag gaggaacaca tgaacatcat tgccattatg ggaccgcatg gcgtctttta
    44281 taaagatgag cccatcaaag aactggagtc ggcgctggtg gcgcaaggct ttcagattat
    44341 ctggccacaa aacagcgttg atttgctgaa atttatcgaa cataaccctc gaatttgcgg
    44401 cgtgattttt gactgggatg agtacagtct cgatttatgc agcgatatca atcaacttaa
    44461 tgaatatctc ccgctttatg ccttcatcaa cacccactcg acgatggatg tcagcgtgca
    44521 ggatatgcgg atggcgctct ggttttttga atatgcgctg gggcaggcgg aagatatcgc
    44581 cattcgtatg cgtcagtaca ccaacgaata tcttgataac attacgccgc cgttcacgaa
    44641 agccttgttt acctacgtca aagagcggaa gtacaccttt tgtacgccgg ggcatatggg
    44701 cggcaccgca tatcaaaaaa gcccggttgg ctgtctgttt tatgattttt tcggcgggaa
    44761 tacccttaag gctgatgtct ctatttcggt caccgagctt ggttcgttgc ttgaccacac
    44821 cgggccacac ctggaagcgg aagagtacat cgcgcggact tttggcgcgg aacagagtta
    44881 tatcgttacc aacggaatat cgacgtcgaa caaaattgtg ggtatgtacg ccgcgccatc
    44941 cggcagtacg ctgttgatcg accgcaactg ccataaatca ctggcgcatc tgttgatgat
    45001 gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat gcgttgggga ttctgggggg
    45061 gatcccgcgc cgtgaattta ctcgcgacag catcgaagag aaagtcgctg ccactacgca
    45121 agcacaatgg ccggttcatg cggtgatcac caactccacc tatgatggtt tgctctacaa
    45181 caccgactgg atcaaacaga cgctggatgt cccgtcgatc cacttcgatt ccgcctgggt
    45241 gccgtatact cattttcatc caatctacca gggtaaaagt ggtatgagcg gcgagcgtgt
    45301 tgcgggaaaa gttttcttcg aaacgcaatc gacccacaaa atgctggcgg cgttatcgca
    45361 ggcttcactg atccacatta aaggcgagta tgacgaagag gcgtttaacg aagcctttat
    45421 gatgcatacc accacctcgc ccagttatcc cattgttgct tcggttaaga cggcggcggc
    45481 gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt tcggtggagc gggcgctgca
    45541 ttttcgcaaa gaggtccagc ggctgcggga agagtctgac ggttggtttt tcgatatctg
    45601 gcaaccgccg caggtggatg aagccgaatg caggcccgtt gcgcctggcg aacaatggca
    45661 cggctttaac gatgcggatg ccgatcatat gtttctcgat ccggttaaag tcactatttt
    45721 gacaccgggg atggacgaac agggcaatat gagtgaggag gggatcccgg cggcgctggt
    45781 agcaaaattc ctcgacgaac gtgggatcgt agtagagaaa accggccctt ataacctgct
    45841 gtttctcttt agtattggca tcgataaaac caaagcaatg ggattattgc gtgggttgac
    45901 ggaatttaag cgctcttacg atctcaacct gcggatcaaa aatatgctgc ccgatctcta
    45961 tgctgaagat cccgatttct accgcaatat gcgtattcag gatctggcgc aagggatcca
    46021 taagctgatt cgtaaacacg atctttccgg tttgatgttg cgggcattcg atactttgcc
    46081 ggaaatgatc atgacgccac atcaggcatg gcagagacag attaaaggtg aagtagaaac
    46141 cattgcgctg gaacaactgg tcggtagagt atcggcaaat atgatcctgc cttatccacc
    46201 gggcgtaccg ctgttgatgc caggagaaat gctgaccaaa gagagccgca cggtactcga
    46261 ttttctactg atgctttgtt ccgtagggca acattacccc ggttttgaaa cggatattca
    46321 cggcgcgaaa caggacgaag acggcgttta ccgcgtacga gtcctaaaaa tggcgggata
    46381 acttgccaga gcggcttccg ggcgagtaac gtgctgttaa caaataaagg agacgttatg
    46441 ctgggtttaa aacaggttca ccatattgcg attattgcga cggattatgc ggtgagcaaa
    46501 gctttctact gcgatattct tggtttcacg ctgcaaagcg aagtctatcg cgaagcgcgc
    46561 gactcgtgga aaggggattt ggcgcttaat gggcaatatg tgattgagct tttctctttt
    46621 ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg gcctgcgtca tctggctttt
    46681 agcgttgatg acatcgatgc ggcagtggcg caccttgaaa gccataacgt gaagtgtgaa
    46741 gccatccgtg tcgatccata cacgcaaaaa cgcttcacct tctttaacga tccggacggg
    46801 ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc cgcatttgcc cggtaacgtg
    46861 ccgggcattg ctactgtaaa atcgcaccat catgacactc acgctcaata gacaacttct
    46921 cacctcacgc cagattctgg tggcctttag cggcgggctt gactccaccg ttctgctgca
    46981 tcagttggtg cagtggcgga cggaaaatcc gggcgtcact ctgcgcgcta ttcatgtgca
    47041 tcacggttta agtgccaatg ccgatgcctg ggttacgcat tgcgaaaacg tctgccaaca
    47101 gtggcaggtg ccgctggtgg tcgaacgcgt acaacttgcg caagaggggc tggggattga
    47161 ggcccaggcg cggcaggcac gttatcaggc atttgcccgt accctattgc ccggcgaagt
    47221 gctggtcacc gcgcaacatc tcgacgatca atgtgaaacc tttctgctgg cgctaaaacg
    47281 cggtagcggc cctgccgggc tttcggctat ggcggaagtt tcggagtttg ctggaacacg
    47341 gcttatccgc ccgttgcttg cccgcacgcg gggggaactg gagcagtggg cattggcgca
    47401 tggtttacgc tggattgaag acgaaagtaa tcaggacgac tcatacgatc gtaactttct
    47461 gcgcctgcgc gtagtgccgt tattgcagca gcgttggccg cattttgcgg aagcaacggc
    47521 ccgcagcgcc acactttgtg ctgaacaaga gagtctgttg gatgaactgc tggcggatga
    47581 tttagcacac tgccaaacgt cgcaggggac gctgcagatt gcgccaatgc tggcgatgag
    47641 tgatgcccgt cgcgcggcga ttattcgccg ctggctggca gggcagaatg caccgatgcc
    47701 ttcccgcgac gcgctggtgc gcatctggca ggaagtggcg ctggcgcggg aagatgcttc
    47761 cccctgttta cgtttgggcg cctttgaaat ccgccgctat cagtcgcaac tgtggtggat
    47821 taaatccgtc accgggcaaa gcgaaaccat tgtgctgtgg cagacgtggc tacaaccgct
    47881 ggaattaccg gcggggctgg gaaccgtaca gcttactgcg ggaggcgata ttcgccctcc
    47941 gcgtgcagac gaagcggtca gcgtgcgttt caaagcgcca ggattgctgc atattgtcgg
    48001 gcgcaacggc ggacgtaagc tgaagaaaat ctggcaagag ttaggcgtgc cgccgtggct
    48061 acgtgacacc acgccactgc tgttttatgg agaaacgctg attgcggcgg caggggtatt
    48121 tgtgacgcaa gaaggtgtgg ctgaaggtga gaatggcgta agttttgtct ggcagaaaac
    48181 gcttagttaa gtgaaagccg gataagacgc atcaaacgtc gcatccggcg aaagtcaatc
    48241 aggattcgct taccaccacc gtaccgattt cagggtggct aaagctggta attttatcca
    48301 gacgcagctc gcgggtttcg ccagcggcct cgacgaccag atactccaca tttttgcggg
    48361 agactaaatc actggctttt gcctgcaatt tttcgccatc tttcagctca agtgtcagca
    48421 ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc atcacaatta attggttgat
    48481 acgtatcatt cattgacata atcgctcacc agtaagtttg ccgcagcgta tgctgctttt
    48541 tccctgacag cctcagaaag ggcgtcgtcg gcagccattt cattcagcac tttcaaaacg
    48601 cagcccagcg cgtccggaac gtatcctaag tctccgctgg cgatttccgc gtaccgcttg
    48661 cgtattaact cacaatattt ttccacatgc cctcctgtca gcactctgac ttaaccgtgg
    48721 atgcaagtct aagcctacga agataaactc tgtttcgcaa ggtgactata ccacactcat
    48781 ttctgcaata tcagcgccgc aactgcacgt attccgttac aatggcctcc tgattcgaaa
    48841 ggagttttct tatggcgctt aaagcgacaa tttataaagc gacggttaat gtggccgatc
    48901 tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc gcgccaccct tcagaaaccc
    48961 aggagcgtat gatgctgcgc ttgctggcgt ggctgaaata tgctgatgaa cgtctgcaat
    49021 ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg gctgcgtaac gatcacctgg
    49081 gcattgattt gtggattgag ctggggctgc cggatgagcg acgaattaag aaagcctgca
    49141 cccaggccgc agaagtggcg ctgtttacct ataatagtcg ggcggcgcaa atctggtggc
    49201 agcaaaatca gagcaaatgt gtgcagtttg ccaatctttc cgtctggtat ctggacgatg
    49261 aacaactggc gaaagtaagc gcctttgccg atcgtaccat gacgctgcag gcaacgattc
    49321 aggatggtgt tatctggtta tcggatgata agaataatct ggaagttaac ttaaccgcct
    49381 ggcaacaacc ttcatgattg tgatttcccg acatgttgct attcccgatg gtgagcttga
    49441 gatcaccgcc attcgtgcgc agggcgctgg cgggcagcat gttaataaga cctcaacggc
    49501 tattcatctg cgttttgaca ttcgggcgtc cagcctgcca gagtattaca aagagcgtct
    49561 gctcgccgcc agccatcatt tgatcagcag tgatggcgtg attgtcatta aggcacagga
    49621 ataccgcagt caggaactga accgcgaagc cgctctggcc cggctggtgg cagtgattaa
    49681 agatttaaca aaagaacaaa aagcccgacg acccacgcgg cccacccgtg catcgaaaga
    49741 gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag gcgatgcgcg gcaaagtacg
    49801 cagcggtcgg gaataaaaag aaggaatgga tggtgaaaaa agcgatagtg acagcgatgg
    49861 ctgtaatcag cctctttact ctgatgggat gtaataatcg ggccgaagtc gatacgcttt
    49921 ctccggcgca ggctgccgaa ctgaaaccga tgccgcaaag ttggcgcggc gtgctaccgt
    49981 gtgccgattg cgaaggaatc gaaatctctc tgttcctcga aaaagacgga acatgggtga
    50041 tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc cttcgcttcc tacggtacat
    50101 gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa aggtgaaaag tcatattatc
    50161 gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg caatccaatt gaatcgcagt
    50221 tcaactatac gctggaaccg gcacaatcca gtttacctat gacgccgatg accctgcgag
    50281 gcatgtattt ttatatggct gatgcggcga ccttcactga ttgcgcgacc ggaaaacgtt
    50341 tcatggtagc gaataacgca gagctggagc gtagctacct ggctgcgcgc ggtcacagtg
    50401 aaaaaccggt gttactgtca gtagaaggtc actttacgct tgaggctaat ccggatactg
    50461 gtgcgccaac caaagtatta gcgcccgata cggcgggtaa attttacccg aataaggatt
    50521 gcagtagttt ggggcagtaa acctgcttaa ggcagaaaca aacgcaaaat tgcctgatgc
    50581 gctgcgctta tcaggcctac agcatataat gcaacatttt gaattagcac gattttgtag
    50641 gccggataag gcgtttacgc cgcatccggc attttgtgca cgaggctggc tttatcactg
    50701 agtcagccca acaaagcgac ccgccttgat ataaatccca ggcttcaggt gcccaacata
    50761 ctgtaactgc gtttctggct taaacgcaga gacatcacca atacgcatat gcatcagatc
    50821 ggcagggcta atccagcccg attgcgccag tgtaagtgga tgcccggatt tggcaaatgc
    50881 atcagtgaca aattccgaac aaaaccacga ctttttgtct ccttcgcaca cactgcttaa
    50941 ctgcgctttc gccaggtcgc tgacgcactg ttggcgaaaa tcctcggaga acggattcag
    51001 tgagcacatc tggcgagtca ccataaaggg aataaattcg acaatgccgc gatagttata
    51061 accgctatct ttgattttat tggcaaacgc ggtgatatct gtggcttgtt gcggggtaag
    51121 atccgggact cgtaagacga aaagcttatc actatgcttc atcgcttttt taagggaaac
    51181 aatctggacg ccagcgcctg tcgcttctgc aacgttatta tcacccagaa agattgcaac
    51241 gtgactcaca gaggaagtgc tgaagacgcg gattccaaat gaggttaccc caaggcttga
    51301 ggagaacagc aaatcgccgg gtttgagatc aggtgccgtt atttctttta ttgattgttc
    51361 ggtgaaagag ctttgatgct ggaatttgac agcccatgtt tttgcctcgg catctactgc
    51421 tgtggctgat gggtctggct ggctgatatc aacggtacaa gccgaaagta aaagaaagct
    51481 ggggagaagc aggcggcagt acgcctttgg tttatccatt ttatacaatc catgtaaaaa
    51541 agggccctga aattcaggac cctttctggc atcagccttt aatctgtttc accagatatt
    51601 cgacgatgtc accagtctta attaactgtt tctcgccgtt gcgacgatat ttatattcga
    51661 tatcgtcgtt gtcgaggtta cggtcgccca gcacaatagt gtgcggaata ccgatcagtt
    51721 ccatatcagc aaacatcacg cccggacgct ctttgcggtc atccagcagc acttcgatac
    51781 cttgtgcacg cagttcgctg tacagtttct cagcaagctc ttgtacgcgg aaggatttgt
    51841 gcatgttcat cggcagaatc gccacctgga acggcgcgat agcgtcaggc catacgatgc
    51901 cgcgttcgtc gtagttctgc tcaatcgccg cagctaccac acgcgttacc ccgataccgt
    51961 agcaacccat cgtcaggatt tggttacggc catcttcacc ctgtacggag gctttcagtg
    52021 cttcggagta cttggtaccc agctggaaga tgtgaccaac ttcgatacca cgtttgatca
    52081 gcagcgtacc ctggccatcc gggcttggat cgccagccac cacgttacgg atatctgcaa
    52141 cttccggggt agcgacatcg cgatcccagt tgataccgaa gtagtgttta ccatcgatgt
    52201 tagcaccagc agcgaaatca ctcatcgccg caacggtacg gtcaatcacc accggaatcg
    52261 gcatgtttac cggacccagt gaacccggac cggctttaac cacagcacga atttcttctt
    52321 cggtcgcgaa agtcagcggg cttgcaacct gcggcagttt ttctgcttta acttcgttca
    52381 gctcgtgatc accgcgcacc agcagcgcaa ccagcgggaa gctactgcct tcaaccgctt
    52441 taaccagcag agtcttaacc gttttctcaa tcggcagatt gaactgttca accagttccg
    52501 cgatggtttt cgcgttcggc gtatcaacca gcgtcatttc ctgggtagca gcagcgcgcg
    52561 gttctttcgg cgcgatagct tctgccagtt caatgttcgc tgcatagtca gaggtgtcgg
    52621 agaagaccac atcgtcttca ccgctctgcg ccagcacctg gaattcgtga gaggcgctgc
    52681 cgccgataga accggtgtcg gcctgtacgg cgcggaaatc cagccccatg cggctgaaga
    52741 ttttgctgta ggccgcatac attgcatcgt aggtttcctg cagggattcc tgagaagtat
    52801 ggaaagagta agcatctttc atcaggaatt cgcgggaacg catgacgccg aaacgcggac
    52861 gcacttcgtc gcggaacttg gtctggatct gatagaagtt cagcggcagc tgtttgtaag
    52921 agctaagctc gttacgaatc aggtcagtga taacttcttc atgagttggg ccgagtacga
    52981 acggacgctc gccacggtca acaaaacgca gcagttccgg accgtactgt tcccaacgac
    53041 cactctcttg ccacaaatcg gctggctgaa ccaccggcat cgacacctcg atcgcaccgg
    53101 cgttgttcat ctcttcacgc acgatgtttt cgactttttt cagaacgcgc acgccggtcg
    53161 gcagccaggt atataacccg gaggccagct tgcggatcat cccggcgcgc agcatcagct
    53221 gatggctgat cacctcggcg tcggcaggtg tctccttgag agtggagagc aggtattggc
    53281 tagtacgcat gttgttacgg ttccagttgg aaggtagaac aggctcaagg cgagcctggg
    53341 acaaaaaaag tgatttagtt taccagtgca aaagaaatgt caaaagagaa gggcgtgaat
    53401 ttaacgcggt tccagcgcaa agacttcaaa acctgcgtcg gtgacgcgcc agcgaacgtt
    53461 aaaatcatgt agccagacgg cataggtttt gcccgtttcc tcacctttac gataggccgg
    53521 gcgcgggtcc tgcgccagta cttcgcggat aaacagcgtt aactgcggat aacgcttctc
    53581 cagcgtcaaa agctgctttt cgacctctgc ggtaaaactc accgccatct ctgcagctgg
    53641 cgcactttgc gcatagctgg cactggcatc gggaagcgat tcggcaaagg ggagatacgg
    53701 tttgatatcc actaccggcg taccatcgac cagatccaga ctgccgagct tcagaatcac
    53761 gctgccttta tggcaaacaa cctctttcag ctctaccagc gacatgccaa ttgggttagg
    53821 gcggaaagta gagcgtgttg cgaaaacccc cattctggcg ttaccgccga ggcgcggtgg
    53881 acgcacagtc ggacgccagc cgccttccat cgtttgatga aagacgaaaa ggatccataa
    53941 atggctgaat gcttccaggc cgcgaacggc gtcggcctgg ttgtaggaag ctatgagatg
    54001 cagttcgccg ttggcgcttt ttaccagacc tggctggcgc ggaacggcga atttttcttt
    54061 atagggcgag cgaataacgc ctatttgctc gaactggaaa ctgctcattt cgccgtaatg
    54121 ttaagcgcag agccgataca tacagcctga cgatagcagc ccggcgtacc gctggtgact
    54181 tcgcagctat gcagtaatac agcattggct ttcattttag aggcattgat ttgcatctgc
    54241 ttacgtgcgg ttggaatgct cggcggagag tcctgattag aggcctggca agagtcgcca
    54301 ctgacttcac cgagatcgcg gaacggtttg ccgactaatt cttctgcatt ggtataaatt
    54361 cggaccggcg tggcgcgcgg cgctttcggt tttgcaggct ccgctttcgg ctggggtgca
    54421 gtgctttgaa cgggttcgac aggggatctg cttaacatgg aacagccgct tagcatgagt
    54481 gctactaaac agatcggtaa agcacgcata gtatttcctc aatgtatgat caaaacgtca
    54541 atattgaatc aggagcttgt aaaaatgaca agacgggcaa gcgcccgtcc tgaatgatat
    54601 tacaaattgt ggaaacagcc taaaaattac cagcctttaa cagcgccgcc gttaaacact
    54661 ttgtttgctg cttcgtaaac ttcatcagac tgataagcct ggacgaattt cttcacgttc
    54721 tcggcgtctt tgttatcttc acgcgttacg atcaggttta cgtatgggga gtctttatct
    54781 tcgacaaaga taccgtcttt cgccggagtc aggccaatct ggctggcata ggtggtattg
    54841 ataactgcca gagcgatttg cgcgtcgtcc agagaacgcg gcagttgcgg tgcttccagt
    54901 tcaacaattt tcagattttt tgggttctca acaacatcaa gaacggtcgg cagcaggcca
    54961 acgccatctt tcagtttgat caagcccact ttttgcagca gcagcagtga acgaccaaga
    55021 ttagttggat cgtttggcac ggcaacctgc gaaccatcct gcagttcatc cagtgatttg
    55081 attttcttgg agtaaccagc aatcggataa acaaaagtgt tgcctactgc gaccagtttg
    55141 tagccacgat ctttcagttg ctgatcaagg tacggtttat gctggaaggc gttggcgtcg
    55201 atatcgcctt tgctcaatgc ttcgtttggc agaacatagt cgttgaaggt taccagctca
    55261 acgtccaggc catatttgtc tttcgcaact ttctgcgcga cttctgctac ctgctgttcg
    55321 gcaccaacaa tcacgccgac tttaatgtgg tttggatctt tttcatcctg accgcagcct
    55381 accagtgcca gtgatccaat cagggctccc actgccgcaa aggttttgaa tttgaacgcc
    55441 ataccttatt ccttcttcaa ttatttatgt tgtgttgaac gttacttgcg agtgacagcc
    55501 cggacgatgc ggtcgcctgc aaactgaatt aaataaacca gaatgaccag caataccagt
    55561 accgtattca tcaccgtcgc gttgtagccg atgtagccat actgatagcc aatctgacct
    55621 aaaccaccgg caccgactgc accacccatc gcggaataac cgaccagggt aatcagggtg
    55681 atagttgccg cattcaccag acccggcaac gcttccggta acagcacttt acagacgatc
    55741 tgcattggcg tggcacccat tgcgcgggaa gcttcaatta acccggttgg gatctccagc
    55801 agagcgttct cgaccatacg ggcaataaac ggtgctgcac caacggttaa cggaacaatt
    55861 gctgcctgca aaccaatcga tgtaccgaca ataacgcggg taaacggaat catccatacc
    55921 agcaagataa tgaacgggat ggaacggaaa atgttcacaa tcgcagaaac ggtacgatac
    55981 agcttcgcgt tagcaataat ttgccccgga cgcgtgacat aaagcagaac gccaaccggc
    56041 agaccaatca caaacccaaa aaatccggat acgaaggtca ttgccagcgt ttcccatacg
    56101 ccacgaacca gcagccacat catcggctca gacataaccc agtacctcta cttttacatg
    56161 gtgttcctgc agccaggcaa tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt
    56221 cagcatgatg ccgaatttaa cgccaccggc gtagtccatc tgcgcgctaa taatgttgtt
    56281 gttgacgttg aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg attgaccggt
    56341 aaactccaga cgcagcatcg gcacgcagtc agtaaatggc tccgtttgca gacgttcctg
    56401 gtaatcttcc gggatatcca gatgcagggt cgactgaata aacttctgcg ccagcggcgt
    56461 tttcggatgc gagaacactt cacttaccgt gtcctgctcg atcagttcgc cattgctgat
    56521 gaccgccacg caatcacaaa tacgcttcac aacgtccatt tcgtgggtga tcaacagaat
    56581 cgtcaaaccc agacggcggt taatgtcttt cagcagttcg agaatagaac gtgtcgttgc
    56641 cgggtccagc gcgctggtgg cttcatcaca cagcaatact ttgggattgc tggctaatgc
    56701 acgggcaatc gccacacgtt gtttctgccc accggaaaga ttcgacgggt agctatcatg
    56761 cttatcgcca agaccaacca atgacagcaa ttccgtcacg cgacgtttga tctcgtcttt
    56821 cggtgtgttg tccagctcca gcggcagagc cacgttgcca aaaacagtac gcgaagagag
    56881 caggttaaaa tgctggaaaa tcataccaat ctggcggcga gctttggtca actcggattc
    56941 tgacagcgtg gtcaattcct ggccatcgac cagcacgcta ccctcggttg ggcgctccag
    57001 caagtttaca caacgtataa gcgtactctt acccgcgcct gaggcaccga taacgccata
    57061 aatttgtcca gctggcacat gcaggctgac gttgttcaac gcctggatgg tgcgggtgcc
    57121 ctggtggaac actttggtga tattcgaaag ttttatcatt gattatttat tatcgtcatt
    57181 aagttagtcg tagcatctcg aatgcctgaa acgggcaacg ccgtcaatga aatggatgtt
    57241 aaggcatcca gacgtctaaa tcaatcaggt ttatgcgaag agcactttct tgcaggtcga
    57301 aacatgcgat actagcgtcc catgccttat taaggagcta taaaaggtgg cgaagagcgt
    57361 acccgcaatt tttcttgacc gtgatggcac cattaatgtc gatcacggct atgtccatga
    57421 gatcgacaac tttgaattta tcgacggtgt tattgacgcc atgcgcgagc taaaaaaaat
    57481 gggctttgcg ctggtggtag taaccaacca gtctggcatt gctcgcggta aatttaccga
    57541 agcacagttt gaaacgctga ccgagtggat ggactggtcg ctggcggacc gagatgtcga
    57601 tctggatggt atctattatt gcccgcatca tccgcagggg agtgttgaag agtttcgcca
    57661 ggtctgcgat tgccgcaaac cacatccggg gatgcttttg tcagcacgcg attatttgca
    57721 tattgatatg gccgcttctt atatggtggg cgataaatta gaagatatgc aggcagcggt
    57781 tgcggcgaac gtgggaacaa aagtgctggt gcgtacgggt aaacctatta cacctgaagc
    57841 agaaaacgcg gcagattggg tgttaaatag cctggcagac ctgccgcaag cgataaaaaa
    57901 gcagcaaaaa ccggcgcaat gattaaaaga tgaacggttg aaataaaaat gcatttttcc
    57961 gcttgtcttc ctgagccgac tccctataat gcgcctccat cgacacggcg gatgtgaatc
    58021 acttcacaca aacagccggt tcggttgaag agaaaaaatc ctgaaattca gggttgactc
    58081 tgaaatagga aagtgtgata tacgccacct cgcgacagtg agctgaaagc tgcgtcgcaa
    58141 ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg gattcttaac
    58201 gtcgcaagac gataaatgaa taccaagtct caagagtgaa cacgtaattc attacgaagt
    58261 ttaattcttt gagcatcaaa cttttaaatt gaagagtttg atcatggctc agattgaacg
    58321 ctggcggcag gcctaacaca tgcaagtcga acggtaacag gaagcgcttg ctgcttcgct
    58381 gacgagtggc ggacgggtga gtaatgtctg ggaaactgcc tgatggaggg ggataactac
    58441 tggaaacggt agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct
    58501 cttgccatcg gatgtgccca gatgggatta gcttgttggt ggggtaacgg ctcacc
//
LOCUS       DAZOZX010000010        58038 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZOZX010000010 DAZOZX010000000
VERSION     DAZOZX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.010"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(24..278)
                     /locus_tag="ACHUPT_000568"
     CDS             complement(24..278)
                     /locus_tag="ACHUPT_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0818287.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUPT_000569"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUPT_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0818288.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHUPT_000570"
     CDS             1228..2694
                     /locus_tag="ACHUPT_000570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0818289.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUPT_000571"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUPT_000571"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0818290.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUPT_000572"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUPT_000572"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0818291.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUPT_000573"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUPT_000573"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0818292.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHUPT_000574"
     CDS             5709..7265
                     /locus_tag="ACHUPT_000574"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0818293.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHUPT_000575"
     CDS             7347..9161
                     /locus_tag="ACHUPT_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0818294.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHUPT_000576"
     CDS             9162..10256
                     /locus_tag="ACHUPT_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0818295.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHUPT_000577"
     CDS             10256..11281
                     /locus_tag="ACHUPT_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0818296.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUPT_000578"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUPT_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0818297.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHUPT_000579"
     CDS             complement(12876..13220)
                     /locus_tag="ACHUPT_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0818298.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUPT_000580"
     CDS             complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUPT_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR0818299.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVL
                     VWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVL
                     SYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVR
                     RIGALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVIL
                     DEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYA
                     SRPKKR"
     gene            complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUPT_000581"
     CDS             complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUPT_000581"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0818300.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15622..17382
                     /locus_tag="ACHUPT_000582"
     CDS             15622..17382
                     /locus_tag="ACHUPT_000582"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0818301.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUPT_000583"
     CDS             17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUPT_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0818302.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUPT_000584"
     CDS             complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUPT_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0818303.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19119..19346
                     /locus_tag="ACHUPT_000585"
     CDS             19119..19346
                     /locus_tag="ACHUPT_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0818304.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUPT_000586"
     CDS             19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUPT_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0818305.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21201..21277
                     /locus_tag="ACHUPT_000587"
     tRNA            21201..21277
                     /locus_tag="ACHUPT_000587"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21235..21237,aa:Pro,seq:ggg)
     gene            complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUPT_000588"
     CDS             complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUPT_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR0818306.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24291..24938
                     /gene="narP"
                     /locus_tag="ACHUPT_000589"
     CDS             24291..24938
                     /gene="narP"
                     /locus_tag="ACHUPT_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0818307.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUPT_000590"
     CDS             complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUPT_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0818308.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUPT_000591"
     CDS             complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUPT_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0818309.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUPT_000592"
     CDS             complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUPT_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0818310.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUPT_000593"
     CDS             complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUPT_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0818311.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUPT_000594"
     CDS             complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUPT_000594"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0818312.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUPT_000595"
     CDS             complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUPT_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0818313.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUPT_000596"
     CDS             complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUPT_000596"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0818314.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUPT_000597"
     CDS             complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUPT_000597"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0818315.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUPT_000598"
     CDS             complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUPT_000598"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0818316.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUPT_000599"
     CDS             complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUPT_000599"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0818317.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUPT_000600"
     CDS             complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUPT_000600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0818318.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUPT_000601"
     CDS             complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUPT_000601"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0818319.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUPT_000602"
     CDS             complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUPT_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0818320.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUPT_000603"
     CDS             complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUPT_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0818321.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUPT_000604"
     CDS             complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUPT_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0818322.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37397..37561
                     /gene="yojO"
                     /locus_tag="ACHUPT_000605"
     CDS             37397..37561
                     /gene="yojO"
                     /locus_tag="ACHUPT_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR0818323.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37675..38184
                     /gene="eco"
                     /locus_tag="ACHUPT_000606"
     CDS             37675..38184
                     /gene="eco"
                     /locus_tag="ACHUPT_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0818324.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(38306..39930)
                     /gene="mqo"
                     /locus_tag="ACHUPT_000607"
                     /pseudo
     CDS             complement(38306..39930)
                     /gene="mqo"
                     /locus_tag="ACHUPT_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(40148..41791)
                     /gene="yojI"
                     /locus_tag="ACHUPT_000608"
     CDS             complement(40148..41791)
                     /gene="yojI"
                     /locus_tag="ACHUPT_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0818325.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41867..42517)
                     /gene="alkB"
                     /locus_tag="ACHUPT_000609"
     CDS             complement(41867..42517)
                     /gene="alkB"
                     /locus_tag="ACHUPT_000609"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0818326.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42517..43581)
                     /gene="ada"
                     /locus_tag="ACHUPT_000610"
     CDS             complement(42517..43581)
                     /gene="ada"
                     /locus_tag="ACHUPT_000610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0818327.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43655..44710)
                     /gene="apbE"
                     /locus_tag="ACHUPT_000611"
     CDS             complement(43655..44710)
                     /gene="apbE"
                     /locus_tag="ACHUPT_000611"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0818328.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44822..45943)
                     /gene="ompC"
                     /locus_tag="ACHUPT_000612"
     CDS             complement(44822..45943)
                     /gene="ompC"
                     /locus_tag="ACHUPT_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0818329.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46681..49353
                     /gene="rcsD"
                     /locus_tag="ACHUPT_000613"
     CDS             46681..49353
                     /gene="rcsD"
                     /locus_tag="ACHUPT_000613"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0818330.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49370..50020
                     /gene="rcsB"
                     /locus_tag="ACHUPT_000614"
     CDS             49370..50020
                     /gene="rcsB"
                     /locus_tag="ACHUPT_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0818331.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50220..53069)
                     /gene="rcsC"
                     /locus_tag="ACHUPT_000615"
     CDS             complement(50220..53069)
                     /gene="rcsC"
                     /locus_tag="ACHUPT_000615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0818332.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53344..54120)
                     /locus_tag="ACHUPT_000616"
     CDS             complement(53344..54120)
                     /locus_tag="ACHUPT_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0818333.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54125..55774)
                     /locus_tag="ACHUPT_000617"
     CDS             complement(54125..55774)
                     /locus_tag="ACHUPT_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0818334.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55775..58038)
                     /locus_tag="ACHUPT_000618"
                     /pseudo
     CDS             complement(55775..58038)
                     /locus_tag="ACHUPT_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14198 a        15657 c        14260 g        13923 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccgcagcc agcccgtgga agaaacgcat cacaatcagc tgatcgatgg
    14461 tttgtgccaa cgcacacgcc accgcggcgg cggcaaacac cagcgtcccg ccaagaacca
    14521 ccggcttacg cccgaagctg tctgccatcg gcccgtagat taactgcccc aacgcaaagc
    14581 ccagaatata agtactgagg gtcatctgcg tactgcccgc cgatacgcca aactgcgctg
    14641 aaattaccgg tagcgcgggc agatacatat caatcgacag cggcatcaac atggccagca
    14701 ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca acgggctcct
    14761 gaaagtcatt gaaaagttag acgacgctgg caatttcttc ttcagttaac ggacgatatt
    14821 caccgggggc taaatcagca tccagcgtaa taccgccaat acgttcacga tgcagctcaa
    14881 ccacgtggtt acccacggcg gcgaacatgc gtttcacctg atgataacgc ccttcgctga
    14941 tggtcagacg aacctgcgtt ggggtaatca cttcaagcac cgccggctta gtgagatctt
    15001 tttcgttatg cagctgcacg cctttagcaa attgctctgc cgtatcgtca gctacaggtg
    15061 attccagtgt caccagatag gtcttctcgc aatgatggcg cggagaagta atgcggtgcg
    15121 accactgacc atcatcagtc atcagcacca gaccggtggt atcgatatcc agacgtcctg
    15181 cggcgtgcag tttccacgct accggttcat ccagaaaata gagcaccgtc gggtgatccg
    15241 ggtcgtccgt ggagcaaaca tagccctgag gcttattgag catgaagtaa cgtggaccgt
    15301 gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg ttcagggagc agtttgaacg
    15361 ctgcattacg aacgatttcg ccatcgacgg tgacacgatt gccgcggatt tcacgcccgg
    15421 caatagcacg gctaacgccg agttgctgtg cgataaattt atcaagtcgc atgtgtgtga
    15481 ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa atctgaactg ttgtctgccc
    15541 agtatagcgg tctaataact tccctcaagg gaaaaagaac cgtggcatac tattagcaga
    15601 ataatctacc tacgcgaaac catgattttt acactccgcc catatcagca agaagccgtg
    15661 gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc
    15721 ggcgcaggta aaagcctggt gatagcggaa ctggcacggc tggcgcgtgg tcgcgtactg
    15781 gtgctggcac acgttaaaga actggtggcg caaaaccatg caaagtatca ggcgctgggg
    15841 ctggaagccg atatttttgc cgccgggcta aagcgcaaag agagccacgg taaagtggta
    15901 tttggcagcg ttcagtcggt cgcccgtaat cttgatgcct ttcagggtga attttcgctg
    15961 ttgattgtcg atgaatgcca ccgtattggt gacgatgaag agagccagta tcagcaaatc
    16021 ctcactcacc tgactaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct
    16081 tttcgattgg gcaaaggctg gatctaccag ttccattatc acggcatggt acgcggcgat
    16141 gagaaagccc ttttccgtga ctgcatttat gagctgccgc tgcgttatat gattaaacac
    16201 ggctatctga cgccgccaga acgactggat atgccagtag tgcaatacga tttcagccgc
    16261 ttgcaggcac agagtaacgg gctgttcagc gaagctgatc tcaaccgtga gctgaaaaaa
    16321 cagcaacgca ttacgccaca tatcatcagc cagatcatgg agttcgccga aaagcgcaaa
    16381 ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag agattgtggg attactgcct
    16441 gccgaagatg ctgcactgat tactggcgac aaccccggcg ctgagcgcga tgtgttaatt
    16501 gaagatttta aagcccagcg ttttcgctat ctggtcaacg tcgcagtact gaccaccgga
    16561 tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt
    16621 taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt
    16681 cttgattatg cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc
    16741 aaaagtgaca acgttccggt acaggttttc tgccctgcct gcggttttgc caacaccttt
    16801 tgggggaaaa cgaccgccga cgggacattg attgaacact ttggtcgccg ctgtcaggga
    16861 tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat
    16921 tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga atgtgacacg
    16981 gtactggttg atccggatga tatgttaaaa gcggcgctac gactgaaaga tgcgctggta
    17041 ttacgctgta gcggcatgtc tttgcagcat gggcacgacg agaaaggcga atggttgaaa
    17101 atcacctatt acgatgaaga cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc
    17161 gcccagcgta ccgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc
    17221 cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc aagccttact gcgacatccg
    17281 gattttgtcg tcgcccgcat gaaaggtcag tactggcaag tgcgtgaaaa agtgttcgat
    17341 tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg
    17401 tataaggcga ttgagtatag aatctcgccc gcttttgcat acgcaaagca gatcacttac
    17461 ctgttgctgg gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa
    17521 cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa
    17581 gttcccggca atcatctacg gtggcaaaga agcaccgctg gctatcgagc tggatcacga
    17641 caaagtcatg aacatgcaag ctaaagctga attctacagc gaagttctaa ccatcgttgt
    17701 tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa
    17761 gctgcagcac atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct
    17821 ccggcggggt tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag
    17881 gccagataag gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc
    17941 cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta atggtcaagg
    18001 tatcggttgc tggatcccag aagatgcgtt cacctaacag catcgcatca aagttaatag
    18061 tcaagccacc gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg
    18121 ggaagctctc ttccagttcg tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc
    18181 ttacgcctgc cagctctttc gacagcgatt ccagttcgat ctcttcgcct gcctgcagct
    18241 gctcattaca gtagctataa acctgctggc gcacgttctg tcgctctgct ttatccagtt
    18301 gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc acggttttgc gctttcgcgt
    18361 tcaatccttc gctggccccg aggaaatcca taaagaaatc cgccactttg cgccctactc
    18421 gccctttcag gaaagtgaga tagcgggtgg actctggatt ggtttcccat tcggttaaat
    18481 cgatacgcgc aacaatatcc gcatgattga tatcaagata atgggttggg ttgatgtcga
    18541 gattttcgtt aacgcgcata ctgctcaggt tgctcagaac tgccaccagc aaatactcca
    18601 ccgccagata acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg
    18661 ctaattcgtc acgcaggcga ccggttgccg cgcggctaaa tgccagaaag tcctcttcgc
    18721 cctgacgctg taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag
    18781 ctttattttt ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg
    18841 tcggttccag caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa
    18901 gctggtgcag agcaatctgg ttgatatcca gactcatgat aaactctcct ttaagaccgg
    18961 gcggtattca accaccgcca gttaagcgac gcaataaaag atggcggtca ttttacagaa
    19021 ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt
    19081 aatatgttgc cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca
    19141 gtaatgaaca ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg
    19201 ctccgaccga cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca
    19261 gcattgcccc ggcccaacgc caggcaattg ccaactcttt tgccagcgcc ttacagtcct
    19321 ctatcaacga agacaaagcg cactaaggga aacagataac aggttatggt aactcatcgt
    19381 cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc
    19441 aatattctgc tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgaca
    19501 acgcttgctg gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctagtg
    19561 ttcgccacct acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg
    19621 atgaggtttt tgtccgtcat tctggcgacg gcgggaatga cgctattact gatcgatagc
    19681 gaagtcttta ctcgtttcca tctccatctt aatcccatcg tctggcagtt ggttatcaac
    19741 ccagacgaaa atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt
    19801 ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt
    19861 cgtcgacgct tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat
    19921 gtggtgtata tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac
    19981 ctgccgcttt cgtacccgat gacggcgcga cgtttccttg agaagcatgg cctgcttgat
    20041 gcgcaggagt atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgtacag
    20101 tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt gctgttgatt
    20161 actgtcgatg gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt
    20221 gctgagcaaa atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc
    20281 atctttggcc tgttctatgg catctcgccg agctatatgg acggcattct gtcgacccgt
    20341 acgcctgcgg cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctca
    20401 tcagatggct ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg
    20461 agcgtacgca cccaatctga cgatcagacc gccacgcagt ggatcaactg gctgggccgc
    20521 tacgcacaag aagataaccg ctggttctcg tgggtttctt tcaatggcac taacattgac
    20581 gacagcaatc agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac
    20641 cagatcaaac gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggtggtg
    20701 attatcactg ccggtcgggg tattccactg agcgaagagg aagaaacctt tgactggtcc
    20761 cacggtcatc tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt
    20821 aatgctctga ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctgcatgtc
    20881 agcacacctg ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat
    20941 tactgggtta ctgctgcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg
    21001 gtgctgaaca ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat
    21061 gaaaaaccac agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct
    21121 aactgattaa ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg
    21181 tagtattagc agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg
    21241 tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa atcccaagaa aaaaccaacc
    21301 cttacggttg gtttttttat atctgcaatt aattcgataa acagaccgtg acacatcaca
    21361 gcctgtttat tttctgttat cagaacgtcc agaccacacc cgcctgagta ttccacggcg
    21421 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
    21481 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
    21541 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
    21601 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
    21661 ccgcttcagc gcgtaatatc cgggtatttc ccacatccgc gcgcatgccg tttgataacg
    21721 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
    21781 atccgtcaac ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat
    21841 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
    21901 ctttcaccac cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
    21961 gggtatagct atcgatatta ccttttccgc cgcgatcaaa accaatatca gaatgagagt
    22021 aaccaaagaa caagccgcga attgtactgc tttcttcacg ggagaaacgg ctatcgatac
    22081 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
    22141 tgcgggtgtt aattgccgaa ctccacatca ccgtatcgta actaacgcct tttacgctac
    22201 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gcggctgcca
    22261 tgttcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcg aggctccagc
    22321 tatggttgcc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
    22381 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
    22441 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
    22501 cggtgacgtt gagctgatcg ctctgatgat tggccatatc ggtacgcata taaaaattgc
    22561 cgtttcccga cagggtattg gtggtcaacg taatgaaatt ttccgcactt gccgtcacag
    22621 atgaacgagt cactggctca gccatcataa tcacatttgc gccggacatc gcaaggttgg
    22681 ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc
    22741 tattttcgag cgaaacgtcc gctttgctgg tatccacggc accatgcgtt ctaaaagaag
    22801 cgccctcaga aaccactacc ttacccacca acatagcggg tgcataattt atttgcggct
    22861 tcatgacctc aagaatgcca tcattccctc tgactgacac gttaaccatt gtgccagccc
    22921 agacattagc gcggccattg ttgattacca tattttcaga gatggctttt gaggaatacg
    22981 tgatacttcc attctggtct gatcgaccga gatcatatac cgcatttgcc tgcactttcg
    23041 taccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
    23101 cctgcgtgtc ctccataaca atgagcccgg aaccatcatc cagttcataa ttttttgaca
    23161 caccatcctt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
    23221 ttgagacaat taatttcccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
    23281 taacctccag taagccgcca ctatccacag tagtattata agcgaagtca ttttcttcaa
    23341 ctcgcaaact tccgccgttt tccaacaaca tattattagc aataccattc ttgatatcga
    23401 actgaccgag acggtttgtt ccgaatacct ccatggcccg cgtggttgtt ttaatagcac
    23461 cgcctgtttt ctgatctacc gcaagcgcaa atcccccctc tttaacctcc agtagtcctc
    23521 ccttttcgat agcggcaaaa acggcccccc catctttttc aacgattaag cgcccgcctt
    23581 taatcgtggt atcattcgct gggccataaa cctgtagcac accattattg agggtagtct
    23641 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatact gagtattctg
    23701 cggtaccatt catttcaatg tattgatacc ctccgttaat ttcagtatta ctacttacac
    23761 caaattcttt tatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
    23821 aaaccagctg gatatctttt tccaggataa caccatcaac tgtctcacca tactcaactg
    23881 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
    23941 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgca
    24001 gttaaaagca ttgggtacag aaaataccca tagctccata cccggagtca gtttttaaaa
    24061 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
    24121 agctgatgca aaattccgtc tttataataa aaatgatgcc aaagcgaacg acaaggttgt
    24181 agttttcact acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
    24241 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
    24301 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
    24361 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgaa ggcgcgagcg
    24421 cgatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
    24481 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
    24541 ttatcctgac cgtatccgat gcctccagcg atgtctttgc gctgatagac gcaggcgcag
    24601 acggttatct gttgaaagac agcgacccgg aagtattgct ggaagcgatt cgtaccggag
    24661 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacgtgaa cgtgaaatgt
    24721 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
    24781 agctggcaca ggggctgtca aataaacaga ttgcatcggt gttgaatatt tccgagcaga
    24841 cagtaaaagt acatattcgc aatctgctgc gtaaactcaa tgtccgctca cgcgtggcgg
    24901 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggatttatca
    24961 tcgggctgag atttatgaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    25021 gtggatcgat caatttattg aatttacaca attttgtagg ccggataagg cgttcacgcc
    25081 gcatccggca taaactaagc gcactttgtc atcagtctag cccgatggca tcaccatcgg
    25141 gcctcttttt atttactctc ctgcggcgac aaatgttgca tcgcctgcgc gatactacgt
    25201 tcaatcaccg cacggcgagt atcgttggca ggtaagagtt tcaacatcat ctcccacgcg
    25261 gcaacggctt cgccaaatcg ctgctgctca aaggcattaa acgcatacat gcttagcaca
    25321 cggatattgc tatggtccgt tctcaccagc tgacgtagca gttcaccgcc gaggcggttg
    25381 tcgttgggat cagatgaacg agtcaacgct tcagcgtatc ccagtgcggc atcgctgttc
    25441 ttcggatcga ggcgatacgc agtagcgtat gcatcggtgg cgatactggc gttacccagc
    25501 gccatgccaa cgcggcccaa cataatccag ccttctatat ctcccggatt tttttgcagt
    25561 tgagtacgca tccccagcgc aaggcgcgac atctcttctt cgttgagcgg atcggctttc
    25621 gggtccagcg ccctgtccag caacgccgga gcctgtgccg tggcctgctg ccagattttc
    25681 acctgctgat aattgccagt ctggtagtag ctgacgccag ccacaattaa cgccaccaca
    25741 atacccggca gataaacaac atatccggca cgcttacctt ccggcacgct ttgttcagga
    25801 aacgcttccg gcaccacgcg tacccgacgc cgcgagcggg cgtatatgac ccaaccgcca
    25861 atgccaatag ccactactgg cagcacccac agcagcacgg tcagcggcgt taacggcgga
    25921 tcgtaagtga cgaagttgcc gtaacgcgcc accatataat cgacaatctc ttttttactt
    25981 ttaccttcct gcatcagttc atacactttc tgacgcaggt cggtggcaat catcgagttg
    26041 gaatcggcaa tgctgttgtt ctggcatttc gggcagcgca gttcttcagt gagctgacgg
    26101 aactgttgtt cctgtgcttc atctttaaac tgcaacacgt cgatggtcgc cagcgctgag
    26161 ccggagatca tcagcatcag cacgcccaat aaaaacctca ttgtgcggcc tccttactgt
    26221 atttctccca cagcggcttg atctcttctt cccagacgcg aggattgaga tcgcccgcat
    26281 ggcgatagcg aatgataccg ttgccgtcaa taaggaaggt ttctggcgcg ccatagacac
    26341 cgagatccag ccctaacatg ccgtcgccat caaacaggct tagcgcgtaa ggattgccca
    26401 gctctttcag ccagctgatt gccttctggc gatcgtcttt atagttcatg ccgaccacgc
    26461 gaatgccctg cgctgaaagc tgattcagat attgatgttc cgcacggcag gtcggacacc
    26521 aagtcgccca gacgttaagc agtactggtt tgccctgagt cagcacatcc gcctgataaa
    26581 actgccccgg attgtccagt gattcgagac gaaacttcgg cacaggcttg ccaatgagcg
    26641 ccgattccag attggtcgga tcatcccctt cggcattacg cgccagctgc cacagcagcg
    26701 ccgcggcaat cgccaggaag ataatcaacg gaattaacaa tactttgcgc ttcatacggc
    26761 ctccggcgca gttttttgcg gactcacgcg cttacgatag cgaggatcaa acagacacag
    26821 cagtccgccc aacgccatca tcagcccgcc cgcccaaatc cagcgaacaa atggtttgta
    26881 gtaaagacgc acggcccacg cgccgttttc cagctcttca ccgagcgcgg cgtacaggtc
    26941 acgcgtgatg ccgccgtcaa ttgccgcttc ggtcatcatc gacccggcag tgttgtaata
    27001 acgtttttcc gcatacagca ccgtttccgg tttaccctct cgcgttacgc caatagtcgc
    27061 cacaccgccg cgccagttcg ggccagtcac ctctttgaca tcacggaagg tgaagcgata
    27121 ttcatgaata tcgacgctat cgccggactt catgcgcaca tcacgctcaa cgctataatt
    27181 ctggctaaag gcaatgccaa caattgtcac tgccagcccc aggtgagccg ccaccatccc
    27241 ccaataactg aaggtggttt tcgtgccgcg tgaaatacgc agcgcagctt ccgcagttgc
    27301 cagcaccgca atccagcagg ccattgccag gccgagcacc gtcatcgcca caactttgct
    27361 ttcgaacagc cacggcaaca gcagagacag caccagcgta gagatgaagg cgataatcaa
    27421 taaattgcgg attttacgtg ggcgatcgcg cccccagcgc accagaggac cgacaccaag
    27481 cagtagcgca aacggcacca tcagccaggt aaacatggtg ttgaagaacg gttcgccaat
    27541 cgaaatactg cccagtccaa gttgcttgtg caccagcggc agcagcgtcc ccagcaacac
    27601 caccagcatc gcggcgacca gcaaaacgtt gttcgctaac agcaaagatt cccgcgacca
    27661 cagcgcattg tttacgcgtg agcgaacttt gtgtccacgc gcggcaaaca gcagcagcga
    27721 accgccaatc accagcacca taaaggcgag gataaacata ccgcgcgccg gatcagacgc
    27781 aaacgcgtgt accgatacca gcacgccgga acgcacgagg aaagtcccca acagacacaa
    27841 cgagaaggca ctgattgcca gcagtaatgt ccacgctttg aagctggcgc gttgttcagt
    27901 gaccgccagt gagtgcatca gtgcagtccc caccagccac ggcataaacg aggcgttttc
    27961 taccggatcc cagaaccacc agccacccca gccgagttcg taataggccc atgcggaacc
    28021 gagcacaatg ccgagcgtta ggaaaatcca cgccgccagc gtccacgggc gagtaaaacg
    28081 cgcataagtg ctgtccagac gcccgctcag caaagaagca atggcaaaag caaacgccac
    28141 cgagaaaccc acgtacccca tataaagcag aggcggatgg aagatcagcc ccggatcctg
    28201 taacagcggg ttaagatcgc gcccttcaat cgggaagttc ggcagcgtgc gtgagaacgg
    28261 gttagaggta aagagaatga acagcaaaaa gccgacgctg accatcccca ttatcgccag
    28321 tacacgggcc acaatatcca gcggaatacg ctgactaaaa atcgccaccg caaaggtcca
    28381 gccgctcatc agcagcaccc acagcagaag cgaaccttca tgcgcgcccc aagtagccgc
    28441 cacgcgatac cacaccggaa gctgggtatt ggagttgctg gcaacatagg tgacggtaaa
    28501 gtcgttgacc acgaaagcat tgaccagcac cagaaatgcg ccagccacag acataaacag
    28561 cagccaggca aacaagcggg aagacgccat catgcgcgca tctccgcgcg ccacgcccca
    28621 tagcggatac acggacagca gcagcgcaat ccctaacgcc aagcacagca gcccgtttcc
    28681 aatttccggc atcatgatgc tgggtcctta taaacactcg ccgggcgacg gtggttagct
    28741 tccatcgctt tctcaacttc tggcggcgtg tagttttcgt cgtgtttcgc cagcacttct
    28801 ttcgcgagga tatgattgcc tttttccagc tcgccctgca ccacaacgcc ctgcccttca
    28861 cggaacagat ccggcaaaat gccttcgtaa gagacatcca ctgagccttc agcatcgtaa
    28921 atggtgaagg tcactttcag cgaattggga tcgcgctgca cgctacccgg catcaccatc
    28981 ccgccaacgc gcagacgctg accgacttcc ggcatctgct gagtttcacg cttgccgtag
    29041 agaatttccc ccggcgtata aaagagatcg atattcgagc gcagcgcata tagcaccaga
    29101 ccgatagtca gcgctagccc tgccaacacg gcacaggcaa tccacaagcg gtttttacgg
    29161 cgaatattca tgcagcctcc tgctgttgcg cagcacgtaa acgcgcctca cgcgcccgct
    29221 gttgcgccac accacgcaga attgcgcgat gttgcatcac cgagtgcacg accaaaacca
    29281 ccagcggaat aacggtcatc accaccgcca gccagacaaa aaaggcgtaa ccgcccattg
    29341 cgaaaaattc attccaggaa gcaaatgcag gggtcattta cggcctcttt tcagtatcag
    29401 ttcactcacc cacggacggc gtttttccat cagcaaaatc aaattacgca tccgcatcag
    29461 cgtcagcgtg gcagacagga gcaggaagcc aaaaatcgac cagcgcagcg gcgaacgcat
    29521 cgccggatcg atactttgct gcatccgcgt tgatccctga tgcagggtgt tccaccactc
    29581 cacggagtaa tgaataatcg gcagattcac cacgccaatc agcaccatga tacctgccgc
    29641 acggcccgcc agacggcggt cgtcgaaggc gtgccacagg gcaatcacac ccacatacaa
    29701 aaacagcagc accagttcag aagtcagacg tgcatcccat acccaccagg tgccccacat
    29761 cggttttccc catgcagagc cggtaaccag ggcaataaag gtaaacacgg caccaatggg
    29821 ggccatcgcc gccaccgcca ggttggccat tttcatctgc cagacaaggc caataaacgc
    29881 cgccaccgcc attgatgcat aaatgcccat cgaccagatc gccgcaggca catgcaggta
    29941 gataatgcgg tagctatttc cctgctgata atcagccgga gcaaagccga atccccagat
    30001 ccagccgacg gtaagcacga ccacactggc aattgccagc cacggtataa accagccaca
    30061 gatttgatac agccgtggtg ggatcgccag ttgatgcagt gttttccaca tagtttcgat
    30121 accagactcg aacaaaaatc agtaatccag cgttattgaa tgctgattcg taacgctgcc
    30181 gccgtcgcaa aaggacttaa tgtcgcggtg cctgccagca acgcgcctaa aattgccaga
    30241 tacccatcaa cgggcaaatg catagaagcc gcgtccatcg cggcggtggc aaagatgagt
    30301 aatgggatag tcagcggtaa caccagtatg ctgagcagca caccaccgcg cttaagtccc
    30361 actgtcagcg ccacgcccgg tgcaccgaga aagccaagcg taggcgttcc cagcagcagc
    30421 gtcagcgcca tcacttgcca gccataaaca tccattccca gtagcattgc taccagtggc
    30481 gaaaggatga gtaacggcag accggttacc atccagtgcg ccatcacctt cgccagcaca
    30541 acggcgggca agggtaacgg caacaacatc aattgttcaa gactgccgtc ctgcaaatcg
    30601 tcacggaaca gtcgttccag cgccagcaag gatgaaagca gcgcagcaac ccagataatg
    30661 cccggtgcaa tacgcgccag cagttgcggc tccggaccga tactgagcgg aaaaagggta
    30721 attacaatca ggaagaacca cagcgggttg gcgatttcgg cgctatggcg aaacgctacg
    30781 cgcagttcaa gacggaaaat acgccagaac atcatgcggc cctcgtttgc gtcagtgaaa
    30841 tgcggcgaat tttactttca gcaacgttga gcggctggtg ggtagtcaga atcacaatcc
    30901 ccccctgctc cgtatgctgc gccatacgct gggtcagacg atcgacgccg ttaacgtcaa
    30961 tcgcggtaaa aggctcgtcg aggatccata atgtggcacg ggtcaaccac agacgcgcta
    31021 aagcgacgcg gcgttgttgc ccggcagaaa gttgatttac cggaatatct tcgaatccgg
    31081 caagcccggc ctgcgccaag gcttccagac attgtgcggc atcgccatcg cgatgataaa
    31141 agtgcagatt ttctaacgcc gtcagccggg ttttgatccc cggctgatgg cctatccata
    31201 acagggtttg atggtagctg tcgcgtacct gatgcaaggg ctgcccttgc cagagaacct
    31261 cgccagcgtc agggcgagac aaccccgtca gcaaacggag aagcgttgtc ttccccgcgc
    31321 cgttgctacc ggtgatttgt acccactctc ctgcgttcag cgtaaatgac aagccactaa
    31381 ataaggttcg ttcatcccgc tcacaaagta actctctggc ttcaagcata cccacgcaat
    31441 aaccctgtta aaaacctggc tcgacttcac gcatatcggg cagcttgtgc gcgatccctt
    31501 tatggcaatc aatacaggtt tgcccatctt tcaccgcctg gtcatgcatc ttcgcggcaa
    31561 ccgatttctg ggcggttgta tccatatact cgaagttgtg acagttacgg cactcctgcg
    31621 agttattgtc cttcatgcgc cgccactcat tctgtgccat cgtcagacga tgagcttcaa
    31681 atttctgcgg cgtgtcaata acgccaaaaa ttttaccata cagctcttta cttgctttga
    31741 gcttgcgtat catcttcggc acaaactcgt gcggaacgtg acaatccgga caggtcgcac
    31801 ggacgccgct acggttgttg tagtgcacgg aatccatgta ttcctgatac accgtgttgc
    31861 gcatttcgtg gcagctaatg cagaactctt cggtattggc tttttccatc ccggtgttaa
    31921 agccccccca gaagacgatg ccgccaacaa aaccgatcaa cagcagcgtc cccagcgcca
    31981 gatggctggg ggtacgccac catttccaca ggcgcttaat cagaccaggc ttacggtcag
    32041 aatttcccat aataacctct tatttcccgt aaccttttga tggggtaaag gtattcccca
    32101 cgattggcgc ggtatcggcc tgcggtacgt gacattgcag acagaaataa cgacgcggag
    32161 ccacttccgc gcccactttg ccgtcgctgt ccataaagtg agtaggactg atacgcggcg
    32221 cgccagtggt gcgatagctt tcgacaccgt ggcactgcaa gcagcgattg gtattggtcg
    32281 ttacctgata accttcaacg ctatgcggga tcattggcgg ctgattcaca tagttcagcg
    32341 gcatccgatc ctgctctttt ggcatccgaa tggccccttc ctgcgtcccg gatacttccg
    32401 gcgactggct aaaatcgacg ccattagccg cccaaaccgc gccgcttacc accagggcca
    32461 gcatcgccgt ccattgacac agcgctttct tcaggtcatg gcttttcatg atttcgctcc
    32521 cgaactccat cgtgtagtta ttgtaaatac atcctcagaa cagacatcca cgcagcgacc
    32581 gcacgtcatg caatcgcggc tggtgacctg caccgggctt tgttcatcca gcaccggggc
    32641 acgtagcaca tgcggttccg ggcaaacatg aaaacaatcc atacagcggt tacatttctg
    32701 ccgatcgctg gccgcaacgg taatcacacc tttgctaccc agcacgccat acagcgcgcc
    32761 aaccggacaa atgtgcccgc accagccgtg ttcaacgacc agtagatcaa ataaaaacag
    32821 cgcgagaata agcagcgcgc cgctgccgaa gcccattacc aggctacgcc ccatcagaga
    32881 aacggggttg atccattccc aaatgagcgt gccagttaag gctgatccca ccagaatgac
    32941 caccaacagc acgtagcgaa tgtggcgagg gatcgtcgca gactgattga ggtcaaagcg
    33001 cctgcgtaac cagttcgcta agtcggtaat cgggttcagc gggcagaccc agctgcaaaa
    33061 taatcgcttt cccgccaggg cgtagagcac ggtgataatc accgcaccgg tcaacgccac
    33121 cgtggcaggc agatgaccac tggcaagact ttgcagcgtc atcagcggat cggtcagcgg
    33181 aacggtgtcg aataacaggc tactgctgta gttgccgtgc aagatccaca caccaaacca
    33241 tggaccgctc aaaaacatcc ccagcacgaa gaactgacaa aggcgacgta acaccagcca
    33301 acggtgactg cgccaccagc cttttttctc cagcgcctcg cgcccggcgt cacgtttacg
    33361 atttgccatt gttcccctcc agccagccga agcggtaatg gtgacctaac tcccctttcg
    33421 ccagtgacag cggtaacacc ttgattgccg gttgttccag cacgcatact ttttcgcact
    33481 taccgcaacc ggtacaggcg tcgctgtgaa ccgtcggcag aaagcgggcg tgcttaccgg
    33541 tacgcgtgtt gcgctccagc tccaaggtga tggcctcatc aattttcggg cattcgcgat
    33601 aacaaacatc gcagcgcagc ccctgaaagt tgagacagtt ttcctggtcc accagtaccg
    33661 ccagccccat ccgcgcgtcg tcgatcgatt caatctcacg atccagcgca ccgcttgggc
    33721 acactttggc gcacggaatg tcctcacaca tttcgcaagg aatatcccgt gcgacaaaat
    33781 atggcgtgcc cgccgacaga ccagaggcca gcgtcgccag ttttaaggtg tcgtaagggc
    33841 aagcctgaac acactgaccg caacgcacac aggcactggc aaaggcgttc tcgtttatgg
    33901 ctccgggcgg gcgcaaccgc acgccagatg cgcgtgcggt ttgctgttgt aaccccagcg
    33961 ccacacccac ggcagccagc ccgcctgctg tgcgaacgac atcgcgcaga aagcggcggc
    34021 gaccattttg aggtttcgct gaccgggaca taatggctta caccttctcc agtttgaccg
    34081 cgcacttctt gaagtccgtc tctttcgaga gcggatcggt cgcatccagc gtcagtttgt
    34141 taaccagctg tgcggcgtcg aagaacggca tgtacaccag gccctgtggc ggacggttac
    34201 gaccgcgcgt ttcaacaatc gagatcactt cgccacggcg agaaaccact ttcactttgt
    34261 cgccacggcg cagatcgcgc gctttcgcat ccagcgggtg aataaacaag accgcttccg
    34321 ggaaggcgcg gtgcagttcc ggcacacggc gagtcatact gccggtgtgc cagtgctcca
    34381 gaacgcgtcc ggtagagagc cacaggtcgt actcttcatc cggtgcttcc gccgccggtt
    34441 cgaacggcag tgcgaagatc accgctttgc catccggttt accgtagaac ttatagcctt
    34501 cgcccgcttt cacgtacggg tcgttacctt cgctgtaacg ccactgtgtt tctttaccat
    34561 tcaccaccgg ccagcgcaga ccgcgcgctt tgtggtagtc atcgaacggt gccagatcgt
    34621 gaccgtgacc gcgaccaaac catgcgtact cttcgaacag ccctttttgc agatagaagc
    34681 ccagctcgcg ggattcatcg ttcagctgat cttccgccag ttcggatacc gggaatttgc
    34741 tcacttcggg ggtggcatac agaacttcgt acagcgtttt gccacgcagt tccggtttct
    34801 tcgccagcag ctcttccggc catacttctt cagttttgaa gcggcgggag aactggacta
    34861 actgccagag atccgatttc gcttcgcctg gcgcctgtac ctgctgacgc cagaactgag
    34921 tacggcgttc ggcgttaccg taagcgccct ctttctctac ccacattgcg gtcggcagga
    34981 tcaagtcggc ggccagcgca ctgactgtcg gatacggatc ggagacgatg atgaagttgc
    35041 gcggatcgcg ccagcccggc atacgctctt cattgatgtt cggcccggcc tgcatgttgt
    35101 tggtacacat ggtccagtaa acattcagct tgccgtcttt cagcgcacgg tcttgtgcca
    35161 ccgcatgaag accgattttc gccggaatgg tgccgctcgg gatattccac ttcttctcac
    35221 agatatcacg atgtttctcg ttagttacca ccatgtccgc aggcagacgg tgagcaaagg
    35281 tgcccacttc acgcgcagta ccacacgcag aaggttgccc ggtcagggag aacggaccgc
    35341 aacccggctg ggaaattttg ccggtcagca ggtgcaggtt gtagaccagg ttgttagccc
    35401 acacaccacg ggtatgctgg ttgaagccca tcgtccagta ggagatgact ttcttgttcg
    35461 gatcggcata cagctgcgcc agttgttcta actggtcttt cggcacgccg gtcatttcag
    35521 cggttttttc cagcgtatac tcagcaacga aggctttgta atcttcaaag ctcatcggtt
    35581 cggaggcgtc agaacccgga ttcttcgctg ctttttccag cggatgggtc ggacgtaaac
    35641 cgtagccgat gtccgtcgcc cctttgcgca ggttaacgtg cttgctgaag aagtcctgat
    35701 ttatcgcatt gttttgaatg atatagttgg cgatgtagtt caggatcacc aggtcagatt
    35761 gcggcgtaaa gatgatgccg ttatccgcca gctcgaagct acgatgctgg taggtagaaa
    35821 gcaccgccac ggtgacgttc tggttagaga gacgactgtt agtgatgcgt gaccagagga
    35881 tcgggtgcat ctccgccatg ttagcgcccc acagcacaaa cgcgtcagcc tgctcgatgt
    35941 cgtcatagca gcccatcggc tcatccatac caaaggtacg cataaagcca actactgccg
    36001 acgccataca gtgacgtgcg ttcgggtcga tgttgttcga acggaagccc gctttgaaca
    36061 gcttggacgc ggcataacct tcccagatag tccactgacc agaaccgaac ataccgatcg
    36121 attccggccc tttttctttc agggcggttt tgaatttctc ttccatcaca tcgaaggcct
    36181 gatcccaggt gattggggta aattcgcctt ctttgtcata tttaccgttt ttcatacgca
    36241 gcagcggctg cgtcaaacgg tctttaccgt acatgatctt gggcaggaaa tagcccttaa
    36301 tgcagttcag gccacggtta accggtgcgt ccgggtcgcc ctgacaggcc accacacgcc
    36361 cctgctgcgt tccgaccaga acgccgcaac cagtaccgca gaaacggcac ggcgctttat
    36421 cccatttaat ggcttcctgc tgaccaacaa cggcgcgggc aacgcccggc acgctgagac
    36481 cggcagccgc cgcagcggcc gcaacggcgt tagctttcat aaagctacga cgactgagtt
    36541 tcatggtgtt tcctcacctt gctcttcctg ctggtgataa accagcgaca ccgccagcac
    36601 gccctctacg ttgcgtactg actcaatggt ttggatcagc gtttcgctgt cttctgcttc
    36661 caccaccaca atcaactgac cgctcggcgc gtcgctgaca gcaacttcac agccgggaaa
    36721 ggcgttcagt tgggtgctga tgtctgaaat tcgttcgctt ttggcctgca cgaccaggct
    36781 gcaaacttgc cagttagtgt gcatggagat actccgcagt tatggctgat accggacagc
    36841 tggctgcaca tgctccacat ccgttgcaaa gttggctatt aagttgcgcc tggtagatcc
    36901 cggagagcgt cgggcgaaag ataattgcca ttggctcaca gctatcctga cagcggcggc
    36961 attcaactga ctgatacgcc aggcacgcgt ccccgatggt gaactgcaaa tcccaggccc
    37021 tggtgtggcg cggagaaaat agcgattcgg ggcaagcctg cgcgcaggcg taacagaagc
    37081 tgcactcgtt atttttgaaa ttaacgctcg gatagccacc cgtgccgcgt tgcagaatgt
    37141 tgttttcaca agcattaata caggcgtcgc agcgggtgca atgggtcaga aaatgagatt
    37201 catcaccgga ccagggcgga cggataccgt tactggcttt gcgccagcaa ccagtgagta
    37261 tgccccgacg ggatgcatca atcttcacat tgaccttcca tcattaacgc gctctgaaaa
    37321 ttgagagcga ccaaatagac cgcataatta ataagccatt tttatagccg ctaagatatt
    37381 aaaggatgtg tcaaagatgc ataccccgat cggggtaaaa cctgtagcag gatcaaaaga
    37441 gtggcgggaa gcgtggcaaa aacgggcttt tgctcacatt tcaaatggtt ataaacatat
    37501 ttatatagcg attaattcac cagagatatt tctgctggtt tgctccctca tcagaattta
    37561 acactaaaag agcaggtaaa attgtctgaa tgttctttaa gttattcata aagcaaatta
    37621 ataaatctga tgaatatgtt aaccttcagc gacatcatcg gtgaaaacct ataaatgaag
    37681 aaggaaagca aaaaaatgaa gaccattcta cctgaagtat tgtttgccgc tttcgctacc
    37741 acttccgcct gggcggcaga aagcgtccag ccactggaaa aaatcgcgcc ttatccacaa
    37801 gctgaaacag gcatgaagcg tcaggtgatt cagttaaccc cgcaagaaga tgaatctacc
    37861 ctgaaagtag aactgttaat cggtcagacg ctggaagtcg attgcaattt gcatcgtctc
    37921 ggcgggaagc tggaaagcaa aacgctggaa ggctggggct atgattatta tgtctttgat
    37981 aaagttagct cgccggtttc cacgatgatg gcctgcccgg atggcaagaa agagaagaaa
    38041 tttgtcaccg cgtatctggg cgatgctgga atgctgcgtt acaacagcaa gctgccgatc
    38101 gtggtgtata cgccagacaa tgtagatgtg aaataccgcg tctggaaggc ggaagagaaa
    38161 attgacaacg cggaagttcg ctaaactgtt gtgaagtacg gcaccccgtt ggttggataa
    38221 ggcggttacg ccgcatccaa catctaacgc ccgagccggt tgcctgatgc gacgctggcg
    38281 cgtcttatca gtatacgcca catcattaca acgcaatatc cgccacttct ttttgaacgg
    38341 gttgcggttt caactgcggt ttcggcgtac tatctgctgc ttgcggtttg tcgtatttca
    38401 gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca tcaccgttca
    38461 gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc gggctggaaa
    38521 cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg gttgacgccc
    38581 ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc acttcggtac
    38641 ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca cgctgccccg
    38701 cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc aacgcttcaa
    38761 aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga tcgaaattat
    38821 ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc atcagatccc
    38881 acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca aacagcacta
    38941 cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt ggtgcgccaa
    39001 cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga ttttccgaaa
    39061 caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga atccccgatt
    39121 cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg gcgcggatgt
    39181 tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta tcgtcattac
    39241 gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc ttctgtaagg
    39301 aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt tctgttgcgg
    39361 atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat cttcagagta
    39421 acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc gcaggaaatt
    39481 aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa atgaacgcgg
    39541 agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa tctgaaatgc
    39601 ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt gcggggtgta
    39661 gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt tcgaactctc
    39721 ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct ccagctcgcg
    39781 tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta catcagtttc
    39841 ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc ccacggccat
    39901 cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt agtcgcttta
    39961 ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat ttaaataatg
    40021 tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat tatagggtta
    40081 ttagaaatga agggattttt agcatattga cctgacggca gcagtccgcc aggtcagcgt
    40141 cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc ggcatcgcgc tcttcgcccg
    40201 tcagctcgct aagttgccca ttgcgcattt ccagcaggcg gtcggcgtgg ataaagtaat
    40261 gatcatcatg actgatagcg aaaatagttt tacccatctc ctgcatcagc ggcagcaaca
    40321 cctgataaaa ctcacgacgg aagtgtggat cctgatccac cgcccattca tccagcagga
    40381 taatatcgcg ttcttctgcc agcgccagca acagcgccac gcgttttttc tgcccttttg
    40441 ataacttcag gttaacaata cgcccgttgc ttaactcaag cttatgagcc attttcagct
    40501 gcgccagcca cttctcaacc agttgcgggt tagcgggttg cccctccggc cccagcagtt
    40561 gatcaaacag ccagacatcg gtaaacactg ccgaaaacag tttgcgataa tcttccggtt
    40621 gttcgccgct gacaggtttg ccatccagca agatttcgcc gctttgtggc tgatacaagc
    40681 ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt accgccaatc agaaacagca
    40741 gctcgccacg tttgatggtg agattaatcg gaccaacgga aaacgcgtta tcctgataag
    40801 caaacgtcac gttacgcagc tccagcgttt gccagttggg aaacgcctgc gggcgcggaa
    40861 actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt gttaaacgcc acctgcgccg
    40921 tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg cgtacgcagg aataacagcg
    40981 tcaacgaata ggtcgcagca acgttggtat cagcccaacc gagactgttc gccatccaga
    41041 acaccaggcc gatagcgccc agcatcatga tgtttgacca gttcacggca ctaagatgga
    41101 aggtgtctgc gcgaataata tgatggcgat actcttgcgc atcggggatg tagaggttgt
    41161 taaacacata ctcggcgcgt tcccggttca gagtcagctc tttgcgccct tcaagtacag
    41221 tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag ggtcgccata tgtttgtaca
    41281 cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc catccagata gccgttacca
    41341 gcaacatttt gcccgacagc atccacagat acgccgatga accgatagtg agaatgatcc
    41401 cctgccccag ttccggcaga cgcacaaaag caatggtgat attgcgcaca tcgctggtta
    41461 atcccgccag caacgaggcg ctaccgagtt gttcaatgcg ctcgacgtgg gtatccagaa
    41521 tccgcttgat aaattcacta cgcagtcggt aaacgaagtg atgccccaaa gtggtgagcg
    41581 ccagttgcga tccgagagtg actgccatca acagcagcaa taatcccaga aactccggta
    41641 acaccagcag actggtatcc gccgtttcga taaggcgctg attgataaaa gcaattaagc
    41701 caatgcctaa tgccgcactg gcgaggctta gcgccatcac actgataaat ggccagcgat
    41761 actgccgcca gacaagtaca agaagttcca tgcagaaaac ccggacaatg aattacagcc
    41821 cgcagtttaa acatcttcgc gcgcacagca ataataattc ttatttttat tcttttttac
    41881 ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt gagtggatga aaccccgctt
    41941 tcaacggttg aataccgtga taaaacagcc gcgattcacc gccccatacc accacatcgc
    42001 catgttccaa caacaaacgt ttgagcggat cattacgttt caggccacca aattgaaaaa
    42061 tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg cagatccggt tcgtctttat
    42121 cctgatgcag cgacagtttc gcgccaggag cgtagcggtt gataagacaa gcatctggct
    42181 ggaaatctgg atagcccgcc gccgtagccg cacgttgaca taaatcatga aagctttgtg
    42241 gcatagcggg ccacggttta tttgtttgcg gatcaatggg cgaatagaga taaccttgcc
    42301 gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc caccgacatg gtatatccgc
    42361 ctggagcgac catctggcga aacggcgact ggctggcaac gtcattaata tcgcggatta
    42421 gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac cgcaccagcc gccagtggct
    42481 cttgccacgg ttcagcatcg gcaaacagat ccaacattac ctctcctcat tttcagcttc
    42541 gcggcgcagc agttgcgctt tacgcgacac gccccagcgg taaccggaaa gtgtgccatc
    42601 accacggacc acccgatgac agggtattac gatagccagc ttgttggcgg cacaggcgct
    42661 ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta gccagttgct gataactgac
    42721 ggtttcacca caaggtatcg tgcgcagtgc ctgccagact tgttgctgaa aagcagtgcc
    42781 gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga ttgaggctgg cgatcacttc
    42841 acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg tcggcagcgg gaaacatctg
    42901 ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc aatatcgcgc aaatcccccg
    42961 ctcgctttct gccaccaggc aacgacccag ctcacaatca gccagcgcgt aacgcaccgc
    43021 cagattttcg ccaccgtgac ggaattgttt agccgtcatg cccagcgttt catcggcttt
    43081 gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga atagacgtcg tcacgctctc
    43141 ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag gcctgttgcc aggctttagg
    43201 cgtcattccg gtagtagctt taaacaaccg atgtagatga aatggactca tcgccacctg
    43261 gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc agcagtcgac acgcgtgggt
    43321 gattttatcc aaccgatgtt gccggggatt ggctttgtct ggctgacaac gtttgcaggg
    43381 gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag aaggagacat tttcccgcaa
    43441 ggcatgtctg gcgcggcaag aaggacggca aaagataccc gtggtgcgca cggcgaaaac
    43501 gaattcgccg tcggcattcg ggtcgcgggc taagacagat tgccagcgtt gatcgtcagt
    43561 taagcatgtg gcgtttttca taatcagctc cctggttaag gatagccttt agtctgcccg
    43621 gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt tttttcgctg acaaggaagc
    43681 ttttaaactg tggtgacatc caggttttaa agctatcgcc ttctttggtg atcatataaa
    43741 ccgccagccc ttcccggcga acaacctctt tggctttctc cggcccgagt accatcaagc
    43801 cagtatccca ggcatcggct tccagcgccg tcggagcaat caccgtcacg gataccagat
    43861 tgtgttcgat gggacgcccg gtttgcggat cgataacatg ggaaagacgt ttgccgtcca
    43921 gttcgtaata gttacggtag ctgccagagg tgctaatccc atgaccgttg atatccacca
    43981 cagcctgaac cgcgttttct ttatcggttg gtttttgaat cgctacccgc cacggctggc
    44041 cttcaccgtt cataccacgg ctgttcagcg cgccgcccac cgacaccaga tagcgggaaa
    44101 tcccttcctg ctccatcaag cgtgccaggt gatccgccgc ataaccttca ccgacggtgg
    44161 agagatcgac atataaatcc ggcaggtctt tttgcagata ttgctgatgc gactgattga
    44221 tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc atcgatctgt tcctggctcg
    44281 gaatttgaac cggctgttgt tccgggccaa agccccacag attcaccagc ggcccgacgg
    44341 taatatccat cgcaccatcg gtccttgccc caatgcgcag cgaggtggtg acgatatcgg
    44401 ccatcgcttc acttaccggc cacggcgaca aactttgcga gtcgttaaag cgcatcagcg
    44461 cggaatcttt tttataggtc gaaagcagct gatcgtcggc gtccagctgg gtctgaatct
    44521 tttctttaag ttcggcactg cgtttggcat caatgcccgg gatgctggca cgccagaagg
    44581 tacccatggt tttgccttca agaacggtaa cttcagtagc gtgggttttg gcgggttgtg
    44641 gtttttgatc gcaaccaaca aagaagagcg cggcaaccag cagtgccacg cgggtaaagc
    44701 ttatttccat acgtgattat cctcatgcga acggtcgcaa gagtacacca aaaaactgtg
    44761 tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc tttgttcgat atccatcgag
    44821 attagaactg gtaaaccaga cccagagcta cgatgttatc agtgttgatg ccagcgtcac
    44881 gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc aacgtaggtg gacatgtttt
    44941 tgttgaagta gtaggtcgca ccaacatcaa catatttcag gatatcttcg tcgtcgtagt
    45001 tacgaccatt gatgacaccc aggtttttac ctttagactg caggtaagcc agggacggac
    45061 gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa gttctgtgct ttgttcgccc
    45121 aacccaagtt gcctacgcga gttgcgttgt aggtctgggt gtactgagca gctaggtaga
    45181 tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc agcgcggtcg ccattaccga
    45241 tgtaacgctc atcataacgg ttcagaccga agttctggtc atcagtacgt ttggagctgg
    45301 aaactgcagc accgataccg aagccttcgt aatcataagt aatagaaccg ccaacgccgt
    45361 caccgttctg acgcagagct tcacgaccgt tgttggtcat gccttcacct tccgggctac
    45421 cgtttttgcc ctgatactga acagcgaagt tcagaccgtc aaccagacca aagaagtcgg
    45481 tgctacggta ggttgcgaag ccgttaccac gctgctgcat gaagttgtca gaaccgtaag
    45541 tgtcaccacc gaattctggc agtacgtcgg tccaggaagt tacgtcgtaa actacgccgt
    45601 agttacgacc gtagtcgaaa gaacctacat cctggaattt cagacctgcg aatgccacac
    45661 gggtccagga gttgttttcg ttttcagcgc tgttgccctg gatctgatat tcccactggc
    45721 cgtaaccggt cagctggtca gtaacctgag tttcaccttt gaagccaaga cgcatgtagg
    45781 tctggtcgcc atctacagac ttgtcgtcag agaaatagtg caggccgtct actttaccgt
    45841 acagatctaa tttgttgccg tctttgttgt aaacttcagc agcgtttgct gcgcctgcta
    45901 ccagcagagc tgggaccagg agggacagta ctttaacttt catgttatta accctctgtt
    45961 atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat taatcagtcg
    46021 gcaagtccat tctccccaaa aatgcagaat aatccaacac gaatatgata ctaaaacttt
    46081 taagatgttt catttatcgc tatagatgtt tcaaaatgta aatacaaggg aactttttaa
    46141 gattattgcg gaatggcgaa ataagcacct aacatcaagc aataataatt caaggttaaa
    46201 atcaataact tattcttaag tatttgacag cactgaatgt caaaacaaaa ccttcactcg
    46261 caactagaat aactcccgct atcatcatta actttattta ttaccgtcat tcatttctga
    46321 atgtctgttt acccctattt caaccggatg cctcgcattc ggttttttta cccttcttta
    46381 cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta actatttcac
    46441 aaataattaa catcagcata atttccagca atctttgttt atttgcaatt aatttcgttg
    46501 ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt ccccccatac
    46561 aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt ctggaatttc
    46621 acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc ataaacacga
    46681 atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg gagcattacc
    46741 cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggggtaat ggtacaaagt
    46801 gccgttaacg cttggctgaa agataaaagt taccagattg tcgacattac ccacgcaatc
    46861 caaaagcgcg tcgatacctg gcgttacgtg accaggcaga tctacgacaa cattgccgcg
    46921 acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca ggatgtctac
    46981 tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca cgacaactca
    47041 acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg tgcagaaaat
    47101 gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct gatctcaacc
    47161 ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagtga cattgttgat
    47221 tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg atgaacgcga aagtttttct
    47281 aacatgcgcc gcctagcctg gcagaacggt cattacttta ccttgcgtac cacgttcaac
    47341 cagccgggac atctggcaac ggtcgtggct tttgatctgc cgattaatga tttgatccca
    47401 ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac gggaaacaat
    47461 gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac gaagattgaa
    47521 atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc ttatggcacc
    47581 ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat cggtttgctg
    47641 gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag tacagaaagc
    47701 gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa tgaagagata
    47761 gtctcactgt tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg cactgtcata
    47821 agtaacaaaa ttgccgatca tttgctgccg catctgaatc tgcaaaacat caccaccatg
    47881 gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta tgagatccgc
    47941 atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga tcaggatcgc
    48001 gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa aaaccagcag
    48061 gggcggatga cctttatgaa aaacattggc gatgcgctga aagaacccgc acagtccctg
    48121 gcggagagtg cggctaaact caacgccccg gaaagcaaac aactggcgaa tcaggcagat
    48181 gtgctggtgc ggctggtcga agaaatacag ttagcgaaca tgcttgcgga tgatagctgg
    48241 aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt gccttcagtg
    48301 ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct gaaagcacac
    48361 gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact gatgcaatat
    48421 gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga tgagtcctct
    48481 gaagaccgcc tgacattccg cattctggac acgggagaag gcgtaagtat tcatgaaatg
    48541 gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgttatgg aaaggcggac
    48601 ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca tttaaacatc
    48661 aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct cgccgctgac
    48721 ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat ggtggatgtt
    48781 acttcggcag aaattcggaa tattgtcact cgcctgttag aaaattgggg tgcaacctgt
    48841 atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac ggataatccg
    48901 tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt acgagaaatt
    48961 gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga agcggtctta
    49021 caattaattg aagtgcaact ggcacaggaa gaggtgacag aatcgcctct gggcggagat
    49081 gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac agtaccggat
    49141 gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt agcccaaacg
    49201 gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa gcagttatgt
    49261 gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa atacatcagc
    49321 gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca tgaacaatat
    49381 gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca aatcacttga
    49441 gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac tgatcaacaa
    49501 cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg gcgataagta
    49561 cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc tgtcgatcat
    49621 tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc tggatatcga
    49681 agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg ccgcgctgca
    49741 gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa tcagtgctgg
    49801 tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc tgtttgcgga
    49861 aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa ccatcagtag
    49921 ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac gatatcgccc tgctgaatta
    49981 tctctcttct gtgaccttaa gtccggcaga taaagactaa tcatctgtag gccagataag
    50041 acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta aacgccttat
    50101 ccggcctacg attcccatta tttcaacaga ttacattaaa gtaggccaga taagacgcgt
    50161 cagtgtctta tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc ttatccgtcc
    50221 tacgaatccc gcgatttcct gaccctctcg gcatataccg tcagcgtctg ttttatcaca
    50281 tccagcgtta ccggcttcga gaggcagctg tccataccgg actccagaca ccgctgcttc
    50341 tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc caactgacga
    50401 atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct aagcacgata
    50461 tcaatatgat tcttactaag tacattaagc gcatcgacgc catcattcgc ggttttacat
    50521 tgatagccca acgatcccaa ctgatctgcc agcagacgtc ggttaatcgg atgatcatcc
    50581 acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt tgtccgtcga cggcagagcg
    50641 ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa tgccggtagc
    50701 tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag cggaatacca
    50761 atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact cactacctcg
    50821 tcagtgatta acacatcttc gggagtcggt tcctgccctt cgtatgttgt aacgacgatg
    50881 ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg gaccgccagc
    50941 cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg agcgccgtac
    51001 aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac cgagatatcg
    51061 ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt cccctggaag
    51121 tttcgctgta cgcccgttcc aacctggaag aagggatcaa acaagcgcac cacttctttc
    51181 gccggaatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc atcagcgcga
    51241 acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa caggttggag
    51301 atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc cggttcaata
    51361 aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc ggtgatgtgg
    51421 ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc cgattcaatc
    51481 ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga gttgttcatt
    51541 gccgtcacca gacgatcgac gcctttcggt aactctttgg tttgcaacag atccaggtta
    51601 ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag gaacatcgat
    51661 tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc ttccatcttc
    51721 acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg atagcgcgaa
    51781 tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac aaaattgacc
    51841 tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag catattgaga
    51901 taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg caaaatgcag
    51961 atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc ttccagtcgc
    52021 agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgcgc gagagtaaac
    52081 aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat gcgaatgcgt
    52141 tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg tggcagattt
    52201 ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg ttcctgcatc
    52261 cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag ggtatgacca
    52321 ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa gttctccatc
    52381 cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata aactggcgtc
    52441 aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc tgatccagta gaggttactg
    52501 ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa cgctttcaac
    52561 gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca gaggttatca
    52621 ctgccgatta aaaatacccg gttgagatcg taagccgcag aaaaattatc gcgccagtag
    52681 cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat tgcggaacaa
    52741 tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc tcgtccacgc
    52801 ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt aagctctttc
    52861 atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag attaaattcc
    52921 tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac ggatgaaaaa
    52981 gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga ggctttcagg
    53041 gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc tccgcctcag gtgaccgatg
    53101 gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctgtcac cattgcaata
    53161 atgcgtatcg cttagaaacc attgccttaa ccaggaaagc cggagtagat ggacagttct
    53221 cagtacgaaa aataagaaat gaccaatctg ccgacgcttg ttaatggtta tatcctggcg
    53281 tcggcagatc gcccagcagt cttatctgaa aaaagagaga gccaggttgt gtgaagggat
    53341 ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca gcattacgca tcggtacaat
    53401 aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg atcagcgtca gttgggcggt
    53461 cgtcaattcc gtttcgctgc gcccaccata gtagttgata tacacctgat agcgcccgtg
    53521 aactggtgct ggcatggcga aaatctcggg tccgtacccc gtcgtgacat ccatatccag
    53581 tgcaccactg tttttcagca cggtgttacc gtaccaggcg tgttcgccat cgggcgtgac
    53641 aacatggaga tcgagatcgg tattgtccgt atcccacgag agaaccagcc gtaaacgtgc
    53701 acgaatagtt cctgtacccg gcgttgagta aaactgcatt ttttgccggc tttgcccatc
    53761 cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa atataagggc gtgcaaatga
    53821 accatcggat tcaattctct gcggcatact ggcaccgttg accaccagcc ggccctgctg
    53881 aactttaccc gccgcagcat aattctttat tttgccgcgg atctgcgctg agatattttg
    53941 atcatctgcc atgttgacgg acgacgccgg gtaattgatc gattgtgaaa aactggcatc
    54001 ttcgccctct gccggatgcc agccagagag cggtgcgtca atctcgactt cctgcacacc
    54061 ttcactgtga gcgacaggcg aaagtgccac cagtaacaac ggaagaaaaa tctttcgcat
    54121 agcattagtc cattaataat tgtcgggtca gcgtttcgat atagttctca tcaagcccgg
    54181 tgggataacc atcaaaggcc aagtgcagat actcatgggt taaatccagc cgatcctgaa
    54241 gcgtaaagaa gttacgaata aacagccgtt tctgctgccg atcggtatag gggaaacctg
    54301 agactaaacg acagaccgca aacacgtctg gttcgttgta ccccgtctcg ccttgtaata
    54361 tacgccgcca ctgcggcatt tttttcgcca gccaggcttt cgctttgggt aataactggc
    54421 aggttgagcg tggagctccc cagcggctaa ggctgttgtc gggataagca aacgcgagta
    54481 tctggtcgta acgctccccc tgccccgcct gggccgtggc ttgccgccag gaaagtgtcc
    54541 cttcggtagc gcgactgcca tgataatgaa ccggatcgcc tgcatagatg agctcctgcg
    54601 tccaggcggt catcgttcgc gcccccgtgg tcgctggcga agcagaaaca cgctgcgtgg
    54661 cgctgctgtc ggggatggtc aggcaatcac cttcccgatt ggcgttttgc tgtaaaaaag
    54721 tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg cgtacttttc gcctcgcgat
    54781 ccagaactct ggcaacgtac tcttcacgat cgagatgcga ctgcaatttc agtttgccct
    54841 tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt aatgtggtta ccattagtaa
    54901 acgtcacacg atagcgccca ttaagtacgc tgggttttac tgccgtcgta ctcttttccg
    54961 ctgtgatttt cttcagcgga tagcgggcaa acagctccac ctctacacat tgcccactgg
    55021 caacctgcgt cggcactggc aaaacccgat taagcaccgt cgcataacgg gttagtaccg
    55081 ttttgctggt gccactgcca gtaacccaca gcggtgtacc atcggttaac cagccagcaa
    55141 aaccaccctg acggatttcc tgtttatcat cggcaaacca gctccaggtt ttcacccgta
    55201 cccgactacc gagcatggat gcgacaccga ttttcgcctc gtccagcacc acatccagta
    55261 agacttcctg agctttgttt tgcgcaggta acgtcgctaa tgaatcaagc agcgatttaa
    55321 ccgttaccga ggtttctggc ttcagcgttg taagagacgc taaccacgca ggtgcctgac
    55381 gctgttgcca gtattgcccc cacacatctg cgccaatatg caaccgctgc ggcgcaaaat
    55441 agagcccaca ggaacgtacc agcgcggtat cgcgcgttat gctctcccca gcctgacaac
    55501 aataaacctc ttccggggag tttccgcgac actggtaggg ctgctccggt tgatgggtat
    55561 cctccagcca ggcgtaaaca tacagtttcc acaaactccc taacggcgtg gtgagcgagt
    55621 caggtaatgc tgagactttg gtcacgcctg atgacgacag ctgatacagt tgatcgtgct
    55681 gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc aagcgttggc agagtaacca
    55741 acgccaatag ccagacgatt ctgcgccagt tcacttattt cacctgcatc ccggtccatt
    55801 cactccctgc cgcgacactt tgctgcgcag gtgcatagga acgcacataa cgagcaggag
    55861 gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg gcggaaagtg accgttcccg
    55921 tcagttcttt caccggcacc atataggcca gttcgcccat ttcattacgc gctttttcca
    55981 gcaattgccc ctgctgtttc gcggcgttgg gtttattgac tgaaatgccc catgttgtgc
    56041 gctcaacgtc agctcccggc gggagcggta cttccacctg accatagcgc agaactgcat
    56101 cctgctcgct ggtaagcgta atttcatcga gatacagcgc atcgctgtca atctcattgc
    56161 tggtcaccgg ctgcagagta aagctcatct cttcttcacc ggggataagc cgatacaact
    56221 ggcgttcaac ggtcaccgga atgttacttt gttgagccgt ttttgccggt tcacgccagc
    56281 ggacctgcac atttgcggcg ataattcgtc accaaaagag agaatgtccg gcacacccta
    56341 accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc atgcgcccgc
    56401 aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc agttcatggc
    56461 cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc ggccgtctgg
    56521 gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg attgctgttt
    56581 gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt caactgcgct
    56641 tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg agattccggt
    56701 gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa atcttcatct
    56761 ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt gcacggcaga
    56821 ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat attatccgcc
    56881 tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt tacgccgagc
    56941 gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc gtcaccattg
    57001 ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat cagccgcaga
    57061 cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg atcggcaagc
    57121 gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac cccaccccac
    57181 ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt ttcactactt
    57241 tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc accgccgagc
    57301 atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag ttttgtgcta
    57361 atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc agattgctga
    57421 atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt ctggcgcatc
    57481 tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg actgaagata
    57541 aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact ccacttcatg
    57601 tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc gtcgccgttc
    57661 atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa cgtgaaatac
    57721 gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc cacctcttca
    57781 cgccgtggac gttcaagcat ttttactcgc cgctcgctgc ggttagtcgc gccaggcgca
    57841 accggctcgc tggagagcgc ctggtcatag ctgataaacg acagactgga gctggtacgc
    57901 acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat ttctggttgc
    57961 agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac aggtttacct
    58021 ttcagcgacg aggttaat
//
LOCUS       DAZOZX010000011        55418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZOZX010000011 DAZOZX010000000
VERSION     DAZOZX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.011"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHUPT_000619"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHUPT_000619"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0818335.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHUPT_000620"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHUPT_000620"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0818336.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHUPT_000621"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHUPT_000621"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0818337.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHUPT_000622"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHUPT_000622"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0818338.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHUPT_000623"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHUPT_000623"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0818339.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHUPT_000624"
     CDS             7826..9559
                     /locus_tag="ACHUPT_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0818340.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHUPT_000625"
     CDS             9708..10559
                     /locus_tag="ACHUPT_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0818341.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHUPT_000626"
     CDS             complement(10546..10887)
                     /locus_tag="ACHUPT_000626"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0818342.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHUPT_000627"
     CDS             complement(10889..11767)
                     /locus_tag="ACHUPT_000627"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0818343.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHUPT_000628"
     CDS             complement(11733..14030)
                     /locus_tag="ACHUPT_000628"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0818344.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHUPT_000629"
     CDS             complement(14081..14401)
                     /locus_tag="ACHUPT_000629"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0818345.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHUPT_000630"
     CDS             complement(14416..15495)
                     /locus_tag="ACHUPT_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0818346.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHUPT_000631"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHUPT_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHUPT_000632"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHUPT_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHUPT_000633"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHUPT_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHUPT_000634"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHUPT_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHUPT_000635"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHUPT_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0818347.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHUPT_000636"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHUPT_000636"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0818348.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHUPT_000637"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHUPT_000637"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0818349.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHUPT_000638"
     CDS             25474..25608
                     /locus_tag="ACHUPT_000638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818350.1"
                     /translation="MKEKPKGVNRKMTNGGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHUPT_000639"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHUPT_000639"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0818351.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHUPT_000640"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHUPT_000640"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0818352.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHUPT_000641"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHUPT_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0818353.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHUPT_000642"
     CDS             30120..30728
                     /locus_tag="ACHUPT_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0818354.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHUPT_000643"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHUPT_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0818355.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHUPT_000644"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHUPT_000644"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0818356.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHUPT_000645"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHUPT_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0818357.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHUPT_000646"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHUPT_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0818358.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHUPT_000647"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHUPT_000647"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0818359.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHUPT_000648"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHUPT_000648"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0818360.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHUPT_000649"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHUPT_000649"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0818361.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHUPT_000650"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHUPT_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0818362.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHUPT_000651"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHUPT_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0818363.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHUPT_000652"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHUPT_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHUPT_000653"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHUPT_000653"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0818364.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHUPT_000654"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHUPT_000654"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0818365.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHUPT_000655"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHUPT_000655"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0818366.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHUPT_000656"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHUPT_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0818367.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHUPT_000657"
     CDS             complement(44843..45142)
                     /locus_tag="ACHUPT_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0818368.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHUPT_000658"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHUPT_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHUPT_000659"
     CDS             45895..46494
                     /locus_tag="ACHUPT_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0818369.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHUPT_000660"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHUPT_000660"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0818370.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHUPT_000661"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHUPT_000661"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0818371.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHUPT_000662"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHUPT_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0818372.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHUPT_000663"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHUPT_000663"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0818373.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHUPT_000664"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHUPT_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0818374.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHUPT_000665"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHUPT_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0818375.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHUPT_000666"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHUPT_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0818376.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHUPT_000667"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHUPT_000667"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0818377.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHUPT_000668"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHUPT_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0818378.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13492 a        15017 c        14165 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatggtgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAZOZX010000012        53211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZOZX010000012 DAZOZX010000000
VERSION     DAZOZX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.012"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(12..323)
                     /locus_tag="ACHUPT_000669"
     CDS             complement(12..323)
                     /locus_tag="ACHUPT_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0818379.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="ACHUPT_000670"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUPT_000670"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="ACHUPT_000671"
     CDS             702..1055
                     /locus_tag="ACHUPT_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR0818380.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="ACHUPT_000672"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="ACHUPT_000672"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR0818381.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="ACHUPT_000673"
     CDS             complement(2811..3281)
                     /locus_tag="ACHUPT_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0818382.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="ACHUPT_000674"
                     /pseudo
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="ACHUPT_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4120..4338)
                     /locus_tag="ACHUPT_000675"
     CDS             complement(4120..4338)
                     /locus_tag="ACHUPT_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0818383.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="ACHUPT_000676"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="ACHUPT_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0818384.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACHUPT_000677"
     CDS             complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACHUPT_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0818385.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACHUPT_000678"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACHUPT_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0818386.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="ACHUPT_000679"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="ACHUPT_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0818387.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="ACHUPT_000680"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="ACHUPT_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0818388.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACHUPT_000681"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACHUPT_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0818389.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACHUPT_000682"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACHUPT_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0818390.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="ACHUPT_000683"
     CDS             14911..15288
                     /locus_tag="ACHUPT_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0818391.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="ACHUPT_000684"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="ACHUPT_000684"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0818392.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="ACHUPT_000685"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="ACHUPT_000685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0818393.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="ACHUPT_000685"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="ACHUPT_000686"
     CDS             18099..18428
                     /locus_tag="ACHUPT_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0818394.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="ACHUPT_000687"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="ACHUPT_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0818395.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="ACHUPT_000688"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="ACHUPT_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0818396.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="ACHUPT_000689"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="ACHUPT_000689"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR0818397.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="ACHUPT_000690"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="ACHUPT_000690"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0818398.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACHUPT_000691"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACHUPT_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="ACHUPT_000692"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="ACHUPT_000692"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0818399.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACHUPT_000693"
     CDS             complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACHUPT_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0818400.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACHUPT_000694"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACHUPT_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0818401.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="ACHUPT_000695"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="ACHUPT_000695"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0818402.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACHUPT_000696"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACHUPT_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0818403.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="ACHUPT_000697"
     CDS             complement(31205..31999)
                     /locus_tag="ACHUPT_000697"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0818404.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="ACHUPT_000698"
     CDS             32137..32478
                     /locus_tag="ACHUPT_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0818405.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACHUPT_000699"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACHUPT_000699"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0818406.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="ACHUPT_000700"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="ACHUPT_000700"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0818407.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="ACHUPT_000701"
     CDS             36394..37686
                     /locus_tag="ACHUPT_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0818408.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37717..37814
                     /locus_tag="ACHUPT_000702"
                     /pseudo
     CDS             37717..37814
                     /locus_tag="ACHUPT_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="ACHUPT_000703"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="ACHUPT_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0818409.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="ACHUPT_000704"
     CDS             complement(38219..38677)
                     /locus_tag="ACHUPT_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0818410.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="ACHUPT_000705"
     CDS             complement(38674..39591)
                     /locus_tag="ACHUPT_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0818411.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="ACHUPT_000706"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="ACHUPT_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0818412.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="ACHUPT_000707"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="ACHUPT_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0818413.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACHUPT_000708"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACHUPT_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0818414.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACHUPT_000709"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACHUPT_000709"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0818415.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACHUPT_000710"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACHUPT_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /gene="ybbA"
                     /locus_tag="ACHUPT_000711"
     CDS             43550..44236
                     /gene="ybbA"
                     /locus_tag="ACHUPT_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0818416.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /gene="ybbP"
                     /locus_tag="ACHUPT_000712"
                     /pseudo
     CDS             44233..46648
                     /gene="ybbP"
                     /locus_tag="ACHUPT_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACHUPT_000713"
     CDS             complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACHUPT_000713"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0818417.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="ACHUPT_000714"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="ACHUPT_000714"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0818418.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="ACHUPT_000715"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="ACHUPT_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR0818419.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="ACHUPT_000716"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="ACHUPT_000716"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR0818420.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="ACHUPT_000717"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="ACHUPT_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR0818421.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="ACHUPT_000718"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="ACHUPT_000718"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR0818422.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53211
                     /locus_tag="ACHUPT_000719"
     CDS             52939..>53211
                     /locus_tag="ACHUPT_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR0818423.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12830 a        13689 c        14016 g        12676 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtgc
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccag gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgaactatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctag ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgcgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaattagcga gaatttactg gcgtaatgtg tgctcttccg ccagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctagactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgaaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggctcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagttattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc attcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DAZOZX010000013        52777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZOZX010000013 DAZOZX010000000
VERSION     DAZOZX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.013"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..356
                     /locus_tag="ACHUPT_000720"
     CDS             <1..356
                     /locus_tag="ACHUPT_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0818424.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="ACHUPT_000721"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="ACHUPT_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0818425.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="ACHUPT_000722"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="ACHUPT_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0818426.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="ACHUPT_000723"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="ACHUPT_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0818427.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="ACHUPT_000724"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="ACHUPT_000724"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0818428.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="ACHUPT_000725"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="ACHUPT_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0818429.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="ACHUPT_000726"
     CDS             6879..7283
                     /locus_tag="ACHUPT_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0818430.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="ACHUPT_000727"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="ACHUPT_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0818431.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="ACHUPT_000728"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="ACHUPT_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0818432.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="ACHUPT_000729"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="ACHUPT_000729"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0818433.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="ACHUPT_000730"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="ACHUPT_000730"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0818434.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12136
                     /locus_tag="ACHUPT_000731"
                     /pseudo
     CDS             10706..12136
                     /locus_tag="ACHUPT_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            12206..12982
                     /gene="yaaA"
                     /locus_tag="ACHUPT_000732"
     CDS             12206..12982
                     /gene="yaaA"
                     /locus_tag="ACHUPT_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0818435.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13135..13431)
                     /locus_tag="ACHUPT_000733"
     CDS             complement(13135..13431)
                     /locus_tag="ACHUPT_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0818436.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="ACHUPT_000734"
     CDS             complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="ACHUPT_000734"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0818437.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="ACHUPT_000735"
     CDS             complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="ACHUPT_000735"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0818438.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="ACHUPT_000736"
     CDS             complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="ACHUPT_000736"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0818439.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18354..18473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="ACHUPT_000737"
     CDS             complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="ACHUPT_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0818440.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18688..19374)
                     /locus_tag="ACHUPT_000738"
     CDS             complement(18688..19374)
                     /locus_tag="ACHUPT_000738"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0818441.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19774..19914)
                     /gene="yjjY"
                     /locus_tag="ACHUPT_000739"
     CDS             complement(19774..19914)
                     /gene="yjjY"
                     /locus_tag="ACHUPT_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0818442.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20010..20726
                     /gene="arcA"
                     /locus_tag="ACHUPT_000740"
     CDS             20010..20726
                     /gene="arcA"
                     /locus_tag="ACHUPT_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0818443.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20785..22137)
                     /gene="creD"
                     /locus_tag="ACHUPT_000741"
     CDS             complement(20785..22137)
                     /gene="creD"
                     /locus_tag="ACHUPT_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0818444.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22195..23619)
                     /gene="creC"
                     /locus_tag="ACHUPT_000742"
     CDS             complement(22195..23619)
                     /gene="creC"
                     /locus_tag="ACHUPT_000742"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0818445.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23619..24308)
                     /gene="creB"
                     /locus_tag="ACHUPT_000743"
     CDS             complement(23619..24308)
                     /gene="creB"
                     /locus_tag="ACHUPT_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0818446.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24321..24794)
                     /gene="creA"
                     /locus_tag="ACHUPT_000744"
     CDS             complement(24321..24794)
                     /gene="creA"
                     /locus_tag="ACHUPT_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0818447.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25005..25874
                     /gene="robA"
                     /locus_tag="ACHUPT_000745"
     CDS             25005..25874
                     /gene="robA"
                     /locus_tag="ACHUPT_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0818448.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="ACHUPT_000746"
     CDS             complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="ACHUPT_000746"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0818449.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26570..27090
                     /gene="yjjX"
                     /locus_tag="ACHUPT_000747"
                     /pseudo
     CDS             26570..27090
                     /gene="yjjX"
                     /locus_tag="ACHUPT_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="ACHUPT_000748"
     CDS             complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="ACHUPT_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0818450.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="ACHUPT_000749"
     CDS             complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="ACHUPT_000749"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0818451.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29739..31406
                     /gene="ettA"
                     /locus_tag="ACHUPT_000750"
     CDS             29739..31406
                     /gene="ettA"
                     /locus_tag="ACHUPT_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0818452.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31513..31680)
                     /gene="nadS"
                     /locus_tag="ACHUPT_000751"
     CDS             complement(31513..31680)
                     /gene="nadS"
                     /locus_tag="ACHUPT_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR0818453.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="ACHUPT_000752"
     CDS             complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="ACHUPT_000752"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0818454.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32967..34349)
                     /gene="radA"
                     /locus_tag="ACHUPT_000753"
     CDS             complement(32967..34349)
                     /gene="radA"
                     /locus_tag="ACHUPT_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0818455.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34398..35366)
                     /gene="serB"
                     /locus_tag="ACHUPT_000754"
     CDS             complement(34398..35366)
                     /gene="serB"
                     /locus_tag="ACHUPT_000754"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0818456.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35472..36116
                     /locus_tag="ACHUPT_000755"
     CDS             35472..36116
                     /locus_tag="ACHUPT_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0818457.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36144..37159
                     /gene="lplA"
                     /locus_tag="ACHUPT_000756"
                     /pseudo
     CDS             36144..37159
                     /gene="lplA"
                     /locus_tag="ACHUPT_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37191..37454
                     /locus_tag="ACHUPT_000757"
     CDS             37191..37454
                     /locus_tag="ACHUPT_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0818458.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="ACHUPT_000758"
     CDS             complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="ACHUPT_000758"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0818459.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="ACHUPT_000759"
     CDS             complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="ACHUPT_000759"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0818460.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="ACHUPT_000760"
     CDS             complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="ACHUPT_000760"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0818461.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="ACHUPT_000761"
     CDS             complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="ACHUPT_000761"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0818462.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42152..43693
                     /locus_tag="ACHUPT_000762"
     CDS             42152..43693
                     /locus_tag="ACHUPT_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0818463.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43665..44528
                     /locus_tag="ACHUPT_000763"
     CDS             43665..44528
                     /locus_tag="ACHUPT_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0818464.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44742..45524)
                     /locus_tag="ACHUPT_000764"
     CDS             complement(44742..45524)
                     /locus_tag="ACHUPT_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0818465.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45521..46594)
                     /locus_tag="ACHUPT_000765"
     CDS             complement(45521..46594)
                     /locus_tag="ACHUPT_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0818466.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46716..46877)
                     /locus_tag="ACHUPT_000766"
     CDS             complement(46716..46877)
                     /locus_tag="ACHUPT_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0818467.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="ACHUPT_000767"
     CDS             complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="ACHUPT_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0818468.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="ACHUPT_000768"
     CDS             complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="ACHUPT_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0818469.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="ACHUPT_000769"
     CDS             complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="ACHUPT_000769"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0818470.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="ACHUPT_000770"
     CDS             complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="ACHUPT_000770"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0818471.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50779..51192)
                     /gene="holD"
                     /locus_tag="ACHUPT_000771"
     CDS             complement(50779..51192)
                     /gene="holD"
                     /locus_tag="ACHUPT_000771"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0818472.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51295..52326
                     /gene="rsmC"
                     /locus_tag="ACHUPT_000772"
     CDS             51295..52326
                     /gene="rsmC"
                     /locus_tag="ACHUPT_000772"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0818473.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52603..52689
                     /locus_tag="ACHUPT_000773"
     tRNA            52603..52689
                     /locus_tag="ACHUPT_000773"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52637..52639,aa:Leu,seq:cag)
BASE COUNT        12562 a        14274 c        13239 g        12702 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc
    11581 ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
    11641 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
    11701 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt
    11761 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
    11821 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
    11881 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
    11941 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
    12001 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
    12061 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
    12121 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
    12181 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
    12241 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
    12301 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
    12361 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
    12421 gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
    12481 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
    12541 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
    12601 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
    12661 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc
    12721 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
    12781 gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg
    12841 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg
    12901 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    12961 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    13021 ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac
    13081 gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    13141 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    13201 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    13261 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    13321 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    13381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    13441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    13501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc
    13561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    13621 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga
    13681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    13741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    13801 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    13861 cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    13921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    13981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    14041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    14101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag
    14161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    14221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    14281 gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc
    14341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    14401 gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    14461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc
    14521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag
    14581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    14641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    14701 gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat
    14761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    14821 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    14881 ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc
    14941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    15001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag
    15061 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    15121 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac
    15181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    15241 agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    15301 ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc
    15361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    15421 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    15481 gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt cattcatcgc
    15541 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt
    15601 cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca
    15661 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    15721 tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    15781 atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    15841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    15901 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    15961 acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt
    16021 tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat
    16081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg
    16141 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    16201 taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    16261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    16321 ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc
    16381 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    16441 ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    16501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat
    16561 gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    16621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    16681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    16741 ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga
    16801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc
    16861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    16921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    16981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    17041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    17101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    17161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    17221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    17281 taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc
    17341 caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    17401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    17461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    17521 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    17581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    17641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    17701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    17761 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    17821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    17881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    17941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    18001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    18061 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    18121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca
    18181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    18241 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    18301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    18361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    18421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    18481 tgtgtactct gtaattttta tctgtttgtg cgctatgcct atattggtta aagtatttag
    18541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    18601 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    18661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    18721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    18781 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    18841 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    18901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    18961 atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag
    19021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    19081 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc
    19141 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    19201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    19261 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    19321 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    19381 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    19441 aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct
    19501 aaactatttt ctgactgcac taacggttta attgttaaaa aatgctacat atccttctgt
    19561 ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag
    19621 ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac
    19681 gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt
    19741 agttttattg ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat
    19801 gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat
    19861 gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg
    19921 ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt
    19981 cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag
    20041 acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt
    20101 tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg
    20161 tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg
    20221 agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc
    20281 tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga
    20341 cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac
    20401 gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt
    20461 tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc
    20521 acttctgtga aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg
    20581 gccgtgagct gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac
    20641 atttcgaatc tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc
    20701 gcttctgcgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt
    20761 tagcgccgtt tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac
    20821 ttctttactg gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac
    20881 aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc
    20941 ggcagagttg agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa
    21001 catgctattg cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc
    21061 aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa
    21121 gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg
    21181 ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat
    21241 ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac
    21301 gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag
    21361 attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg
    21421 tttggctggt agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat
    21481 ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc
    21541 catattcagc tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc
    21601 ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc
    21661 aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt
    21721 gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt
    21781 tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac
    21841 gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt
    21901 ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat
    21961 aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc
    22021 gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa
    22081 cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt
    22141 cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt
    22201 gaagtgacgg tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt
    22261 gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct
    22321 tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta
    22381 gtcaggaata ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc
    22441 ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt
    22501 ccccagcgcc tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac
    22561 atgcaaagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa
    22621 taatgccgcc acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg
    22681 gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt
    22741 aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat
    22801 cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt
    22861 tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct
    22921 actaccgaga tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt
    22981 gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat
    23041 ccccaacaaa atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc
    23101 catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat
    23161 cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg
    23221 cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc
    23281 aacggcttta tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg
    23341 atattcattg cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag
    23401 ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg
    23461 cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac
    23521 gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag
    23581 taaaaaatag cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat
    23641 cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca
    23701 cgcagcgttt taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac
    23761 acgctatcca ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc
    23821 agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc
    23881 gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc
    23941 ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta
    24001 gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg
    24061 gccgtcagga acagtacagg taacgccgga tagagcgcca gtaactggcg gcacaattca
    24121 aagccactga tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga
    24181 gctttatcca gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc
    24241 aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc
    24301 cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg
    24361 tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt
    24421 ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg
    24481 aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg
    24541 acttgctgac aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta
    24601 ataccaccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca
    24661 aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa
    24721 ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa
    24781 tgcttgtatt tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg
    24841 taaataacat attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa
    24901 catactctac taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat
    24961 tacctgatgt caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca
    25021 ttattcgcga ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca
    25081 atgtagcggc gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca
    25141 ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac
    25201 tacgcctgac tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac
    25261 agacatttac ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt
    25321 ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc
    25381 cagagcacaa atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact
    25441 cctgttcgct ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg
    25501 attttctcgg caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc
    25561 cgagtcagga taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc
    25621 aggcagatgg ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga
    25681 tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa
    25741 cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc
    25801 cggcagaaga cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc
    25861 ctatccgccg ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc
    25921 ctgcggtttc aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg
    25981 aaatagaaca gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg
    26041 tactcaccag acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg
    26101 gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact
    26161 cgccttcagg aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt
    26221 cttcttcggt cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca
    26281 ggcgagaatc aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc
    26341 gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg
    26401 ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa
    26461 tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc
    26521 tttttttccg tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt
    26581 tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat
    26641 cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca
    26701 accctttggc agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg
    26761 tttacttcca gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac
    26821 tttcagttgg gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt
    26881 gccgttacct gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag
    26941 tcgcgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc
    27001 gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg
    27061 tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac
    27121 attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc
    27181 gcttttcagc aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct
    27241 gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg
    27301 actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg
    27361 ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt
    27421 cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga
    27481 atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc
    27541 ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga
    27601 cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa
    27661 gcatcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg
    27721 acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca
    27781 ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca
    27841 tattgcaggt aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg
    27901 ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc
    27961 tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga
    28021 cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa
    28081 agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc
    28141 gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc
    28201 tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg
    28261 agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg
    28321 tcaacattct gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct
    28381 gcaaccatcg ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca
    28441 gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct
    28501 tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca
    28561 agcgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca
    28621 tcgcgccatt tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata
    28681 tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc
    28741 atcagccgtg cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt
    28801 tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg
    28861 ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc
    28921 agcactgtta ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac
    28981 gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta
    29041 ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc
    29101 ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc
    29161 gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga
    29221 gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc
    29281 accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc
    29341 ggataatcct tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag
    29401 gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg
    29461 ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct
    29521 ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata
    29581 gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac
    29641 tgcgaaggtg atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa
    29701 aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca
    29761 tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga
    29821 gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc
    29881 tgctgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag
    29941 acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg
    30001 agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt
    30061 atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc
    30121 tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg
    30181 cggatgcgct gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc
    30241 gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg
    30301 aaccgaccaa ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact
    30361 tcgaaggcac cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct
    30421 ggatcctcga actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc
    30481 tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt
    30541 cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta
    30601 aagcacgtct ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa
    30661 ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca
    30721 acctgcgtaa atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga
    30781 aaggggcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta
    30841 tgatctccgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa acggtgaaac
    30901 tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag
    30961 tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg
    31021 ttggccgctt taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg
    31081 gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc
    31141 tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc
    31201 tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg
    31261 ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta
    31321 ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta
    31381 tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta
    31441 tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg
    31501 gtaagcgtcg catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg
    31561 gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa
    31621 accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat
    31681 tttatacctc gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg
    31741 caccagctcc acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa
    31801 ttcgatattg ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac
    31861 cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat
    31921 caccagatcg aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc
    31981 gtactttttg cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt
    32041 ggcgtatttc actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc
    32101 ggaatactgc aatgcgattt catcaccgcc gaggtgtgaa aagacataat cgcggccata
    32161 ttcccacgca ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc
    32221 gctcgactcg ccgccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg
    32281 aatatattcc cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa
    32341 ggtacgtttc ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc
    32401 atcggcttct tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt
    32461 tttgatgccg ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt
    32521 gaaagcatga atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg
    32581 atccggcacc gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt
    32641 atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag
    32701 gtagatatgt ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt
    32761 ctgccgggga aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg
    32821 gcttttaatt ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc
    32881 agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga
    32941 cgacatatct ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag
    33001 agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc
    33061 ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg
    33121 ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc
    33181 agcggtctgt cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt
    33241 tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga
    33301 ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact
    33361 gccacgcggc gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc
    33421 aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg
    33481 cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg
    33541 aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa
    33601 cgggagtcgg catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc
    33661 gggccagcca gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc
    33721 gttttggcga aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc
    33781 gatgattgta catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt
    33841 tgctcttctt cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga
    33901 ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg
    33961 ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc
    34021 gtggatttcc ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg
    34081 cctcctagta cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg
    34141 ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga
    34201 cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag
    34261 gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa
    34321 acaaaggcgc gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca
    34381 ggatgacggg caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc
    34441 atcaggtcag cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg
    34501 taggcaatcc ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc
    34561 gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct
    34621 ttgtactgcg cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc
    34681 tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag
    34741 aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc
    34801 agctgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg
    34861 tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg
    34921 cgcatcgccc gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt
    34981 tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc
    35041 gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg
    35101 cgtgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac
    35161 caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt
    35221 ttatccagcc cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc
    35281 atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc
    35341 aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa
    35401 ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca
    35461 aagggtggaa gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc
    35521 tgttctgtct tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc
    35581 accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga
    35641 cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga
    35701 tcctcgatca gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag
    35761 gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg
    35821 gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg
    35881 gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac
    35941 aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg
    36001 tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc
    36061 tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca
    36121 ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg
    36181 acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc
    36241 gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt
    36301 ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg
    36361 gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca
    36421 agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg
    36481 gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc
    36541 gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct
    36601 tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac
    36661 tggcggcgaa aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc
    36721 cggggctcac catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg
    36781 cgagcacgtg gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc
    36841 cgaaaccttt gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc
    36901 gcatctgctg gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa
    36961 aggccatatc acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc
    37021 cctcgccggg cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga
    37081 agcgctgttg gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat
    37141 agcgggggcg gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc
    37201 tggaaaacca ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag
    37261 ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct
    37321 cgattaattc tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca
    37381 aaatggcaga aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg
    37441 ctgaacataa atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat
    37501 atgaaaaata atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc
    37561 ggagcagtcc cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc
    37621 tttatcgccc agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg
    37681 acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt
    37741 cagggctttc gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac
    37801 gccgaggatg ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag
    37861 gtcagcggag aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac
    37921 tgcgttacgc accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat
    37981 gcggttaact ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac
    38041 gtgcggcaga actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt
    38101 gatcagttct ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga
    38161 aattttgcgg cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc
    38221 acgggcatct tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat
    38281 caaaactacg tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt
    38341 atcctttttg tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg
    38401 gctttgccat attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc
    38461 gcgaaggttt cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc
    38521 ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg
    38581 gtgaggatca ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg
    38641 aacagttcca gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg
    38701 aagtcaacga agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg
    38761 gtggcgtcaa acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg
    38821 gcgtagatgt ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt
    38881 ttctgcagca cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc
    38941 tggaagttac cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag
    39001 ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg
    39061 aaagtttctt catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc
    39121 ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg
    39181 cagttaccga ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc
    39241 gggaagctgt tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg
    39301 atttcccagt gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac
    39361 gcgccgataa cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg
    39421 tgtgctttcg ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca
    39481 ttatcagctt cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg
    39541 tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata
    39601 aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta
    39661 taccgttatt cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca
    39721 agtttaattg ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg
    39781 aggataaccg ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta
    39841 aagccgacgc tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact
    39901 actgccatcc ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag
    39961 actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc
    40021 gggccttttt gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc
    40081 accgcctgca atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac
    40141 atctccacgc acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc
    40201 aggaactgca ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga
    40261 ttcatgtcgg tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc
    40321 gcttcggcaa gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc
    40381 acgtccatca ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg
    40441 atcagcggga tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca
    40501 gccggagcca gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg
    40561 cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc
    40621 gtaccgccag tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct
    40681 gcgaccatcg gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta
    40741 tcaacaatcg ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc
    40801 atcgccatgg tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc
    40861 gcgagggcgg caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa
    40921 cgaatttctt catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg
    40981 agaaacaagg taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac
    41041 gccttatccg gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc
    41101 gtaaagcatc ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc
    41161 agcaggcttg ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca
    41221 gcaccgaaca gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg
    41281 ccgcccgccg gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc
    41341 atgatgcgag cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg
    41401 aagtccgcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc
    41461 agttcgccgg tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta
    41521 caggctttca ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg
    41581 aacaccacgt caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg
    41641 atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg
    41701 gtgccctgct ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata
    41761 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg
    41821 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    41881 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    41941 ttagtattct aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct
    42001 acttacaagt ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat
    42061 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    42121 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    42181 acaacgttgc cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt
    42241 tctggcactg gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg
    42301 cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    42361 ctatgtctta cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga
    42421 aggcgcgaaa gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc
    42481 gtcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    42541 tagaattcta acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga
    42601 cagaaccctg ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac
    42661 ccgcgcgatc ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc
    42721 tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa
    42781 accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat
    42841 tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa
    42901 cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc
    42961 gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca
    43021 atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt
    43081 tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga
    43141 agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag
    43201 cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc
    43261 catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt
    43321 gccgtatggc gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc
    43381 ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc
    43441 gcaggcactg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc
    43501 caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt
    43561 agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc
    43621 acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg
    43681 ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc
    43741 tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac
    43801 gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg
    43861 acggcaaagt ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt
    43921 gtccgcaaca tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg
    43981 tgcgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc
    44041 aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc
    44101 tcacctgtct ggtggacagt aacggtatgt tgagcgaaac cggctgggaa aaattactcc
    44161 cggtgtgtga cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat
    44221 taaccggacg cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca
    44281 agctggcgga actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg
    44341 aagaactggc ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc
    44401 atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg
    44461 agccgttggc tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct
    44521 atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg
    44581 cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc
    44641 cggcctacgg ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg
    44701 cattatgcgc cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac
    44761 accgcatacg tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac
    44821 tcgcaaagca cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa
    44881 ccgttgagcg gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt
    44941 gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt
    45001 ttgtagccca gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca
    45061 acaccagcac gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc
    45121 cgtgaatgca ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt
    45181 aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc
    45241 accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca
    45301 tcgctatgtt tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa
    45361 ttttccgcta acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc
    45421 ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc
    45481 gggaaatcaa aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt
    45541 taaatgtcgg gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg
    45601 cgactggcgc aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg
    45661 taagcggcgc tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata
    45721 aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat
    45781 gtaatggctt cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa
    45841 attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca
    45901 ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt
    45961 gtgacggcac agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa
    46021 ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc
    46081 tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg
    46141 ttggtaaaaa gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt
    46201 tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg
    46261 cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga
    46321 aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc
    46381 agataaacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat
    46441 taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc
    46501 cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa
    46561 tattaccaag cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa
    46621 agaaagacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac
    46681 tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac
    46741 aaactcacta ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg
    46801 gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg
    46861 atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag
    46921 cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt
    46981 tcgggaggac gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg
    47041 ctttggcgat actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct
    47101 gaaccacgcc atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca
    47161 gcagtttggc tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc
    47221 cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt
    47281 tcgccacttt cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca
    47341 cgactttttg atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca
    47401 gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca
    47461 tagagctatc gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt
    47521 aggcagagcc ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct
    47581 tcagtcttgt catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca
    47641 acacgaggtt gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc
    47701 catcacaaaa gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat
    47761 atcggtagaa tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta
    47821 acgccatcag aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg
    47881 atatcagcga aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata
    47941 aggaagggaa attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc
    48001 gcgggtctgg tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat
    48061 gctggtagcg atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt
    48121 acgcttgaac tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac
    48181 gttgactgac tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac
    48241 atcaaactgc agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa
    48301 cacctgcacc gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt
    48361 cagcggatct ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt
    48421 gaacttcatc atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg
    48481 caggccgagg atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa
    48541 ggtcagcgcg tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc
    48601 tttttcatat ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc
    48661 catgttggcc tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt
    48721 acgggtatcg gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat
    48781 atgatcaacg ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc
    48841 aaggaacagc tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg
    48901 cagctgctgt gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt
    48961 aacaatgcgg acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc
    49021 tttataaagg tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg
    49081 tgcgcagcca attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat
    49141 atcacggtca agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc
    49201 catcagctta cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca
    49261 gtccaccgcc gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc
    49321 gagcaggtta accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat
    49381 cccacgctgc ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc
    49441 ttttactgta ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt
    49501 agttttaccg gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt
    49561 caaataagga gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt
    49621 gttgaaaacg aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt
    49681 acacaggagc tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc
    49741 tggttgctcg cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc
    49801 gagaatatcg gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc
    49861 cgcctgttcc agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc
    49921 ggaaatcacc agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg
    49981 caaggcgcta aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag
    50041 cagagaaacc gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc
    50101 gttaagcaag cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg
    50161 aagctgtaat gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc
    50221 ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg
    50281 caggccggtg aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc
    50341 ccacttcatt attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct
    50401 tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa
    50461 ctttcgtaca gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc
    50521 acgccgcgtt tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga
    50581 cgctgatagt cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc
    50641 gtaatcgcaa acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg
    50701 ccctggttgc tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg
    50761 tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat
    50821 ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga
    50881 tgccacctga gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca
    50941 gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg
    51001 gctgacggtt aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc
    51061 gtttgccacc atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa
    51121 cgcgccaggg cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg
    51181 tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga
    51241 taccgcgcca acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct
    51301 gcatttaccc cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt
    51361 attctgtttg ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc
    51421 cgtgctcata cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac
    51481 gcccgtttta gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac
    51541 tactggccga agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg
    51601 ccagtgggga cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag
    51661 cagatgctgg cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc
    51721 tattttggtc gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac
    51781 agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat
    51841 gtcggtagcc agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat
    51901 gtcggctgtg gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt
    51961 ctcaccttgt gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc
    52021 aacggtgttg aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt
    52081 gatatgatca tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg
    52141 caaacgctga ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta
    52201 gcgaacgcct tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt
    52261 atcgcgcaaa ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa
    52321 ggttgagtat ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc
    52381 cgacctacaa aacctgcacg ttagcccttc gtaggccaga taagacgcgc cagcgtcgca
    52441 tctggcatct gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt
    52501 tccagcaatt acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa
    52561 tgcgcctccg tggtagcaat tctttttaag aattgatggt atgcgaaggt ggcggaattg
    52621 gtagacgcgc tagcttcagg tgttagtgtc cttacggacg tgggggttca agtccccccc
    52681 ctcgcaccaa aaatcacgtt gatattgctc gcactgggcg aaggtggcgg aattggtaga
    52741 cgcgctagct tcaggtgtta gtgttcttac ggacgtg
//
LOCUS       DAZOZX010000014        49672 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZOZX010000014 DAZOZX010000000
VERSION     DAZOZX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.014"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            124..756
                     /gene="comR"
                     /locus_tag="ACHUPT_000774"
     CDS             124..756
                     /gene="comR"
                     /locus_tag="ACHUPT_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0818474.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(818..1357)
                     /locus_tag="ACHUPT_000775"
     CDS             complement(818..1357)
                     /locus_tag="ACHUPT_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0818475.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1584..2888)
                     /gene="ndh"
                     /locus_tag="ACHUPT_000776"
     CDS             complement(1584..2888)
                     /gene="ndh"
                     /locus_tag="ACHUPT_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0818476.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3296..3838)
                     /gene="ycfP"
                     /locus_tag="ACHUPT_000777"
     CDS             complement(3296..3838)
                     /gene="ycfP"
                     /locus_tag="ACHUPT_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0818477.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3861..4886)
                     /gene="nagZ"
                     /locus_tag="ACHUPT_000778"
     CDS             complement(3861..4886)
                     /gene="nagZ"
                     /locus_tag="ACHUPT_000778"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0818478.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(4897..5721)
                     /gene="thiK"
                     /locus_tag="ACHUPT_000779"
     CDS             complement(4897..5721)
                     /gene="thiK"
                     /locus_tag="ACHUPT_000779"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0818479.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5702..6343)
                     /gene="lpoB"
                     /locus_tag="ACHUPT_000780"
     CDS             complement(5702..6343)
                     /gene="lpoB"
                     /locus_tag="ACHUPT_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0818480.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6358..6735)
                     /locus_tag="ACHUPT_000781"
     CDS             complement(6358..6735)
                     /locus_tag="ACHUPT_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0818481.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6738..7097)
                     /gene="hinT"
                     /locus_tag="ACHUPT_000782"
     CDS             complement(6738..7097)
                     /gene="hinT"
                     /locus_tag="ACHUPT_000782"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0818482.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7430..9615
                     /gene="fhuE"
                     /locus_tag="ACHUPT_000783"
                     /pseudo
     CDS             7430..9615
                     /gene="fhuE"
                     /locus_tag="ACHUPT_000783"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9675..11108)
                     /gene="ptsG"
                     /locus_tag="ACHUPT_000784"
     CDS             complement(9675..11108)
                     /gene="ptsG"
                     /locus_tag="ACHUPT_000784"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0818483.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11403..12200)
                     /locus_tag="ACHUPT_000785"
     CDS             complement(11403..12200)
                     /locus_tag="ACHUPT_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0818484.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12211..13215)
                     /gene="holB"
                     /locus_tag="ACHUPT_000786"
     CDS             complement(12211..13215)
                     /gene="holB"
                     /locus_tag="ACHUPT_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0818485.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13212..13853)
                     /gene="tmk"
                     /locus_tag="ACHUPT_000787"
     CDS             complement(13212..13853)
                     /gene="tmk"
                     /locus_tag="ACHUPT_000787"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0818486.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13843..14865)
                     /gene="yceG"
                     /locus_tag="ACHUPT_000788"
     CDS             complement(13843..14865)
                     /gene="yceG"
                     /locus_tag="ACHUPT_000788"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0818487.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14868..15677)
                     /gene="pabC"
                     /locus_tag="ACHUPT_000789"
     CDS             complement(14868..15677)
                     /gene="pabC"
                     /locus_tag="ACHUPT_000789"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0818488.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15797..17038)
                     /gene="fabF"
                     /locus_tag="ACHUPT_000790"
     CDS             complement(15797..17038)
                     /gene="fabF"
                     /locus_tag="ACHUPT_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0818489.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17125..17361)
                     /gene="acpP"
                     /locus_tag="ACHUPT_000791"
     CDS             complement(17125..17361)
                     /gene="acpP"
                     /locus_tag="ACHUPT_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0818490.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17572..18306)
                     /gene="fabG"
                     /locus_tag="ACHUPT_000792"
     CDS             complement(17572..18306)
                     /gene="fabG"
                     /locus_tag="ACHUPT_000792"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0818491.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18319..19248)
                     /gene="fabD"
                     /locus_tag="ACHUPT_000793"
     CDS             complement(18319..19248)
                     /gene="fabD"
                     /locus_tag="ACHUPT_000793"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0818492.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19264..20217)
                     /gene="fabH"
                     /locus_tag="ACHUPT_000794"
     CDS             complement(19264..20217)
                     /gene="fabH"
                     /locus_tag="ACHUPT_000794"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0818493.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20285..21355)
                     /gene="plsX"
                     /locus_tag="ACHUPT_000795"
     CDS             complement(20285..21355)
                     /gene="plsX"
                     /locus_tag="ACHUPT_000795"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0818494.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21436..21609)
                     /gene="rpmF"
                     /locus_tag="ACHUPT_000796"
     CDS             complement(21436..21609)
                     /gene="rpmF"
                     /locus_tag="ACHUPT_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0818495.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21661..22182)
                     /gene="yceD"
                     /locus_tag="ACHUPT_000797"
     CDS             complement(21661..22182)
                     /gene="yceD"
                     /locus_tag="ACHUPT_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0818496.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22381..22965
                     /gene="yceF"
                     /locus_tag="ACHUPT_000798"
     CDS             22381..22965
                     /gene="yceF"
                     /locus_tag="ACHUPT_000798"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0818497.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23077..24036)
                     /gene="rluC"
                     /locus_tag="ACHUPT_000799"
     CDS             complement(23077..24036)
                     /gene="rluC"
                     /locus_tag="ACHUPT_000799"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0818498.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24609..27800
                     /gene="rne"
                     /locus_tag="ACHUPT_000800"
     CDS             24609..27800
                     /gene="rne"
                     /locus_tag="ACHUPT_000800"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0818499.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27997..28939)
                     /gene="flgL"
                     /locus_tag="ACHUPT_000801"
                     /pseudo
     CDS             complement(27997..28939)
                     /gene="flgL"
                     /locus_tag="ACHUPT_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(28951..30593)
                     /gene="flgK"
                     /locus_tag="ACHUPT_000802"
                     /pseudo
     CDS             complement(28951..30593)
                     /gene="flgK"
                     /locus_tag="ACHUPT_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30659..31600)
                     /gene="flgJ"
                     /locus_tag="ACHUPT_000803"
     CDS             complement(30659..31600)
                     /gene="flgJ"
                     /locus_tag="ACHUPT_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0818500.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31600..32697)
                     /gene="flgI"
                     /locus_tag="ACHUPT_000804"
     CDS             complement(31600..32697)
                     /gene="flgI"
                     /locus_tag="ACHUPT_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0818501.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32709..33407)
                     /gene="flgH"
                     /locus_tag="ACHUPT_000805"
     CDS             complement(32709..33407)
                     /gene="flgH"
                     /locus_tag="ACHUPT_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0818502.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33460..34242)
                     /gene="flgG"
                     /locus_tag="ACHUPT_000806"
     CDS             complement(33460..34242)
                     /gene="flgG"
                     /locus_tag="ACHUPT_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR0818503.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34380..34967)
                     /gene="flgF"
                     /locus_tag="ACHUPT_000807"
                     /pseudo
     CDS             complement(34380..34967)
                     /gene="flgF"
                     /locus_tag="ACHUPT_000807"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(35069..35131)
                     /locus_tag="ACHUPT_000808"
                     /pseudo
     CDS             complement(35069..35131)
                     /locus_tag="ACHUPT_000808"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35151..36290)
                     /locus_tag="ACHUPT_000809"
     CDS             complement(35151..36290)
                     /locus_tag="ACHUPT_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0818504.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(36315..37010)
                     /gene="flgD"
                     /locus_tag="ACHUPT_000810"
     CDS             complement(36315..37010)
                     /gene="flgD"
                     /locus_tag="ACHUPT_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0818505.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37022..37425)
                     /gene="flgC"
                     /locus_tag="ACHUPT_000811"
                     /pseudo
     CDS             complement(37022..37425)
                     /gene="flgC"
                     /locus_tag="ACHUPT_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(37429..37845)
                     /gene="flgB"
                     /locus_tag="ACHUPT_000812"
     CDS             complement(37429..37845)
                     /gene="flgB"
                     /locus_tag="ACHUPT_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0818506.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38000..38659
                     /gene="flgA"
                     /locus_tag="ACHUPT_000813"
     CDS             38000..38659
                     /gene="flgA"
                     /locus_tag="ACHUPT_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0818507.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            38735..39028
                     /gene="flgM"
                     /locus_tag="ACHUPT_000814"
     CDS             38735..39028
                     /gene="flgM"
                     /locus_tag="ACHUPT_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0818508.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39033..39449
                     /gene="flgN"
                     /locus_tag="ACHUPT_000815"
     CDS             39033..39449
                     /gene="flgN"
                     /locus_tag="ACHUPT_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0818509.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39489..41024)
                     /gene="murJ"
                     /locus_tag="ACHUPT_000816"
     CDS             complement(39489..41024)
                     /gene="murJ"
                     /locus_tag="ACHUPT_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0818510.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41191..42057)
                     /locus_tag="ACHUPT_000817"
                     /pseudo
     CDS             complement(41191..42057)
                     /locus_tag="ACHUPT_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(42059..42706)
                     /locus_tag="ACHUPT_000818"
     CDS             complement(42059..42706)
                     /locus_tag="ACHUPT_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR0818511.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42717..43301)
                     /gene="rimJ"
                     /locus_tag="ACHUPT_000819"
     CDS             complement(42717..43301)
                     /gene="rimJ"
                     /locus_tag="ACHUPT_000819"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0818512.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43537..44745
                     /gene="mdtH"
                     /locus_tag="ACHUPT_000820"
     CDS             43537..44745
                     /gene="mdtH"
                     /locus_tag="ACHUPT_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0818513.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44809..45456
                     /gene="grxB"
                     /locus_tag="ACHUPT_000821"
     CDS             44809..45456
                     /gene="grxB"
                     /locus_tag="ACHUPT_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0818514.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            45590..46150
                     /locus_tag="ACHUPT_000822"
     CDS             45590..46150
                     /locus_tag="ACHUPT_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0818515.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46256..47302
                     /gene="pyrC"
                     /locus_tag="ACHUPT_000823"
     CDS             46256..47302
                     /gene="pyrC"
                     /locus_tag="ACHUPT_000823"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0818516.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47376..47621
                     /gene="dinI"
                     /locus_tag="ACHUPT_000824"
     CDS             47376..47621
                     /gene="dinI"
                     /locus_tag="ACHUPT_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0818517.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47911..48165
                     /gene="bssS"
                     /locus_tag="ACHUPT_000825"
     CDS             47911..48165
                     /gene="bssS"
                     /locus_tag="ACHUPT_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0818518.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48280..49398
                     /gene="solA"
                     /locus_tag="ACHUPT_000826"
     CDS             48280..49398
                     /gene="solA"
                     /locus_tag="ACHUPT_000826"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0818519.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            49446..49559
                     /locus_tag="ACHUPT_000827"
     CDS             49446..49559
                     /locus_tag="ACHUPT_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR0818520.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        12081 a        13344 c        12281 g        11966 t
ORIGIN      
        1 tttaaaaatc tttaaatttc agtcaaatag aaagcaatta tttttatggg atctggactg
       61 gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag gtacaacggc
      121 atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc
      181 gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg
      241 acttctcttg cggacctcgt cgaagcaacc ggagccaaag cgcccacgct atacgcggaa
      301 tttaccaaca aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc
      361 gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac
      421 tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg
      481 atcaacaact gcaccaccct ctccccagat tcaggagata tagccaatac gttgaaatca
      541 cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg
      601 gaaatcccga cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag
      661 gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca gattgccgga
      721 acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaggc ccctcagcct
      781 gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca
      841 gttgcccgtt actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc
      901 cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat
      961 acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct
     1021 ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac
     1081 cgcgaccacc accaaactga tgccctatca cgccaccagc aacagcgccg agcaccgacc
     1141 cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact
     1201 cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt
     1261 gcgggccccg ttcaaacacg ttcagactgg ccactgccgc tacgcccaac gcagctgcga
     1321 caccaatccc gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt
     1381 tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg
     1441 gaatgactgg aaatacttaa ggattcggag gagtctgaag gaatgcctga tgcgacgctt
     1501 aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa ggcgtttata
     1561 ccacatccgc cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta
     1621 atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc
     1681 attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg
     1741 cgcgtcaggt tacccatcag gctaccaacg gtggagaagt tcgacagcga taccagcgaa
     1801 ccatgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc cagaatgttg
     1861 ttcattgcgc aagtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct
     1921 tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgagtggtt
     1981 tgcagcgtcg gttccaccac cagctggttg atacggttag tttcaagacc accgatatct
     2041 ttcaggaagt ctggcgcttt gatcccggct gcccatacca tcagatcagc ctcaatatat
     2101 tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc
     2161 acgcgaacgc caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat
     2221 gcaggcaaaa tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg
     2281 cctttgtaac cgtagctgtg cagttgcttg atcgcgttgt gcaattcagc ggagagttct
     2341 acacccgtcg cgccgccgcc gacaatcgca atgttcactt tgccatttgc gcccaggttg
     2401 gcagagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc ctggtgcggg
     2461 ttatcgagga aaatgcagtt ctctttgacg cctggcgtat tgaaatcgtt agaggtgcta
     2521 cccagcgcca tgaccagggt gtcataggcg attttacgtt ccggaaccaa cagttcacct
     2581 ttctcgtcgc gtagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg
     2641 gaacccaact ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg
     2701 acgccttcat caagcgagcc agtcgccact tcgtgcagca gtggtttcca caggtggctg
     2761 tggttacgat cgaccagcgt aattttggct tttttcttac gtcccagctt atgccccagc
     2821 tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatctt tttcaatggc
     2881 gtagtcaacg tgcccccctt aaatttatta accaattgtt aattaatggt tattaacata
     2941 gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg
     3001 aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctt aaaagatagg
     3061 caactttacc gggggagatc aaaaaataga gtttattgtt tattatttga gcaaattcgg
     3121 aagggtaata tagggtgaag cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca
     3181 gcatcgcatc aggcattgat gctcgactgt cggatgcggc gtggacgcct tatccgactt
     3241 acagaattcg gagcgcatca ggtaccggca aggttacctg atgcgacgag ggcatttacc
     3301 cgagggtttt gaacgcttta atgcgctgta aatgcgggga gatattcttg aatttgtgcg
     3361 tctgctcttc gtcccagaca atctcgtaat aatgatgcaa ctcttcagat gtccgctggc
     3421 tgttaagtgc ttcatcatta cgcgacaaaa taaccagaca acgatcgcga ttcttctcgc
     3481 ggaagttggt cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc
     3541 cttccatgtt ctcataaggg aacaaattag ggttgaagat cacctggcgg atatcgcaaa
     3601 gaaaaccaat ccgttccgcc cagtatccgc ccaggccaac gccgcaaatt agcggacgct
     3661 cgtcaacgtt cagttgcaac attttgtcca cttctttaag cagatgctgc atatcatgtt
     3721 tcggatgccg cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta
     3781 agactttctc gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg
     3841 cttcctcaca taagccaggg ttagtgacct actttctctt cctgccagcg ttcatgtaac
     3901 tgattcagac gggtgctgat cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa
     3961 aatgaacctt tatgatacaa acgtgtaaca cgttctgcct tgatcggtga cagattatct
     4021 aacacgctga cggccccttt acgattattg cagaccagga tcatatcgca acccgcatcc
     4081 agtgatgcct gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa
     4141 tcgtcagaga aaatcacgcc gtcaaacccc agttcctgac gcaaaacggt tttcagccag
     4201 tagggagaac cgctcgccgg acgcggatca acatcactgt agatcacatg cgcaggcata
     4261 atggcgtcga gtttattttc gcggattaag gaactgaaga ccgacatatc tttagcgcga
     4321 atctccgctt gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc cgttactgca
     4381 ccgtgtcctg ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat accatcaata
     4441 aaccggctgg caattgccag ggctttttgt ggatcggcat gataagaacg ctcgccaatc
     4501 gccgcgctga tatgcccgac atccagcact ggcgcaaagc tgatatcaat atccatagcg
     4561 atcatttcgc tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt
     4621 cccaacagcg cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc
     4681 tgcacgcgtc caccttcctg atctaccgcc accaccagat gattgcgcga tgctgcgcgg
     4741 atctggcgca ccagttcacg taactgggca ggatcatgat agttacgcgt aaagagaatc
     4801 agccctccca ccagcggatg cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct
     4861 tcgacatcca acattactgg acccacactg ctctccttat tgttttatta atagctgccg
     4921 ccaggtgtca tcggccagcc tgataaattg ttgatcgccg gtttgtcgcc agcggtactc
     4981 aaaccaccct cctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa
     5041 ttgcgccgga taaatcttcg cgcgagtggc atagtcattg accaattgcc ggtgctgttc
     5101 agtattttcc acccacaccg ccgccagttc cagcgcgata tcaccatctc cagcatactc
     5161 ccagtcgatg agttttaacc ctgatgcgct atgcactaaa tttccggcgt ggacatccat
     5221 atgcaatgga cttaagcgta aaggtcgtgg ttcccgcgct ttgcgcagac gttttaacat
     5281 tcgcagccaa cccacagtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa
     5341 gggcaacagc gttattcgcc agccaaaacg tggttgttga tgtagataat acagcaagcc
     5401 tgccagttcg ttggtatccg gcaaatacgt ttttaccgca ccgggcagat agtcgactac
     5461 catccagtca cggagatata aatgcggctt cggtgcaatg cttgcgggta gttgtgataa
     5521 agcccgatac tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac
     5581 aacaaaacgc tggccctgat gttcaatgag aaaactaccg ccactaagcc cactattaaa
     5641 cgtcgtgacg ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg
     5701 attattgctg cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca
     5761 tcagctgcat ttgtagggtc ggagcgttaa cgttgccaga ggcgcaggag tacagcacgt
     5821 aatgagcgcc gacattgcgg gcaatgccta tggctttact acgggtgcct aaactgtcct
     5881 gcggcgacaa acctaactgt tgcttagcca tcgacagttg ctgggcggaa accagggtaa
     5941 atttcccgtt attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg
     6001 aaccgttagt acggttgtta acgctatcaa ccagcaggac gctacccgca gtcaccccgt
     6061 cagccccaag catcttactg accatcggct gcattgcgcc attccagtca taatggcgaa
     6121 tatgcggcgc aggcggtgcc gttcgatctt cgtgctcaat tgggcctggc tgctgcggga
     6181 tcgtcggcac cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg
     6241 ctggtttcac ttcttctacc ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca
     6301 tcgccagcgc ggtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttta
     6361 caaatataga taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt
     6421 taccgccgaa tgtgcgggaa tggtaacgct gcgtggcctt tccagaggat gcatctccag
     6481 ccctctggcg tcataccagt aaaaacgata atgaacggta acgggttctt gcctttcgtt
     6541 ataaatcgtt gaggatgctg aaggttgaat atcagacgtc gaaaggacgg gcttttctgc
     6601 actgatgccc gcagccaata aagatgactc catcaccagc gattgctcat cattcaccgg
     6661 aatttccgga tgtgaacgac agcccaccag cagtaacaac accagagaca ccagcccaaa
     6721 gcatcctttt ctcatcgtta cagaccttta tgcgccagca ttggtcccag cggacggcca
     6781 cccaacaagt gcatatggat gtggtaaacc tcttgtccgc catggcggtt ggtattcatg
     6841 atcagacgat agccatcttc ggcaatacct tcttgctcag caatttttgc cgctacggtg
     6901 atcatgcgtc ccagcgcctg ctcatgctca gctgacacgt cgttcacagt cggaatgaga
     6961 atattcggaa tgatcagaat atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt
     7021 accagatcat cctggtagac gatatcggag gggatctcac gacgaataat tttgctgaat
     7081 atagtttctt ctgccacgac attttccttt ttcaaatagc catgcgttgc gccatgctac
     7141 gccgcgccac actgcgagta tagagtatgg tcgagttaaa cctgctcttt caatcttaac
     7201 tcacgatttc ttaagcaaaa aatcgttttg ctgccgtata tatcgccatt attcccattt
     7261 ctgcctgcga atgcgctgaa aagacattct ctgtttggtt tacacgacaa atatgaatgc
     7321 gtatatttct catttgcatt tgcagcaaaa ttattcgcac actaaatata accgctcatc
     7381 accctcctcc ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac
     7441 acaatttaac agggataatc aatatcaagt catcaccaaa ccgtcactac ttgccggttg
     7501 catagcactg gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt
     7561 gattgttgag ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc
     7621 tacctctgcg ggtaccaaaa tgcagatgac tcaacatgat attcctcagt cggtcactat
     7681 tgttagccag cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa
     7741 cacgctgggg atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt attcccgcgg
     7801 attccagatc gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa
     7861 tctgggcgac gcactttctg atatggcact gtttgaacgc gtagaagtag tgcgtggcgc
     7921 gacaggactc atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca
     7981 cgcgacctgt cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga
     8041 acggtatgtg gcggatttac aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat
     8101 tgtcggcggc taccagaata acgactcatg gctggaccgc tacaatagtg aaaagacctt
     8161 cttctcgggc attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga
     8221 atatcagcgc attgatgtta atagcccaac ctggggcggt ttaccgcgct ggaatactga
     8281 tggcagcagc aacagttacg atcgcgcacg cagtaccgca cctgactggg cgtacaacga
     8341 taaagagatc aacaaggtct ttatgaccct gaagcagcgg tttgctgata cctggcaagc
     8401 gacactgaat gccacccact ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc
     8461 ctatgtaaac aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt
     8521 tgatcatgtc ggcggcaccg gttggaacag tggcaaacgt aaagttgatg cgctggattt
     8581 gttcgctgac ggtagttatg aattgtttgg tcgtcagcac aatctaatgt ttggtggcag
     8641 ttacagcaaa caaaacaatc gttcagttca tgggccaaca tcttcccgga tgaaattggc
     8701 agtttctaca actttaatgg caatttccca caagccgact ggtcatcaca gagcctggcg
     8761 caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat
     8821 ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac
     8881 agcatggaga aaaaccacac cacgccttac gctggtctgg tgtttgacat caatgacaac
     8941 tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca
     9001 ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg
     9061 atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct
     9121 cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat
     9181 gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag
     9241 ctgacatttg gcgcaacgcg ctatcttgca gaggataacg aaggaaacgc cgttaatcct
     9301 aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag
     9361 ttgacagtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg
     9421 tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac
     9481 caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac
     9541 gataccaaca tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag cattaccggc
     9601 acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc
     9661 caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg
     9721 gatttagtac cgaaaatagc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc
     9781 agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac gcgcagacgg
     9841 gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc
     9901 ggtgccattt cgctggtacc tgttgctttt gcatcttcag tcgcgtcttc acgacccggc
     9961 gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatcgcataa
    10021 ccgataccga cgatcggtaa cagccacagt ttgctgctgt taccagacag aacgatgaag
    10081 tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat acagattggg
    10141 aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag
    10201 aagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata
    10261 ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca
    10321 gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca
    10381 cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca
    10441 ccaatctgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccaggcaa
    10501 cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga
    10561 gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta
    10621 aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga
    10681 tactcaggca gcttaatacg gtagaaacgg ttaaacatgt acgctgcaat cgcaccggag
    10741 ataatccctc cgagtacgcc agtatccgcc aagtgtttag aggcgatttc ttcagcaggt
    10801 aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca
    10861 actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca
    10921 aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca
    10981 acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggt
    11041 agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc
    11101 ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct cacgcgtggc
    11161 aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag
    11221 taaaataatt cagtgccaat ttgtttgacg gctatcacgt ttcaacttca gatcattgga
    11281 gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgcggtt attcaccgcc
    11341 caattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc
    11401 attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg
    11461 gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca
    11521 acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa
    11581 ggtgagtcag tttccaccag taatcgatcc agggggacat aacgcgcagc atcgcgcagt
    11641 tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt
    11701 aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc
    11761 actttttctt cgcgcagaat tgccagcgta tcggcacggg cgtcacgggt atggacgata
    11821 accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgacgt
    11881 actttagttt ccggtgtgta ataataatcc agcccggttt cacccagcgc tacaacaccc
    11941 tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga
    12001 tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg catatgtaaa
    12061 taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc
    12121 gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag
    12181 tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta acacaacgcc
    12241 cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat
    12301 gcctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag
    12361 gcacgaggga gaaagatggt ttgccagttc ggcgaccagg cccggcacat caacattggt
    12421 cacctgcgca gcgccatgat ggcgttttag cgcatccatc agcaacgttg ccagccagtg
    12481 taaacgcgcc ggagcttgtt catgattaag ggccgccagc agcgaatacc agtcgcccga
    12541 tggcacgcta tatgccaacg cctgacacaa tgtttcacga gcctgccagt tatctccctg
    12601 aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg caagtaatgc
    12661 atcctgtgac attgtcactt cgcgtgaaag ccaagtcacg gcgtactgtt ctggaggcgg
    12721 cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt caggctcgcg
    12781 ggtagccagg aaaaaccagg tttctgctgg tggctcttca agcgttttca gcaatgcgtt
    12841 agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctag
    12901 gcgtgcgtgc tcattcagct tttcggtgac ctcacgtacc gcatcaatgc ccagcgtatt
    12961 tttccctttt tcgggagcca gggtgtagta atcgggatgc gtgccagcct gcatcaactg
    13021 acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg
    13081 gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagtagcgc
    13141 atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca
    13201 tggataccat ctcatgcgtc caactccttc acccagtggg tcacggtagt gcggattgca
    13261 tccatcacgg cctccagcgg ctgggtggca tcaatggtat gaatactttt atcttgtgca
    13321 gccagttcca gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata
    13381 cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga
    13441 tagagcgtta agtctgggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata
    13501 tgttggtcaa taccacgtcc tccgccctga tacgcctgag tggaaagatc gtggcgatcg
    13561 ccaatcaccc aggtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg
    13621 cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat
    13681 ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga
    13741 gtgaaaacca tgtcgcggat acccagttgc tcgagcgtct caaccaccac attacgcgcg
    13801 gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc
    13861 cttaagcact ttcagataat cctgcacaga cttgttatga ctggcaagat tggtattaaa
    13921 cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc
    13981 agcagccttc agcgaatccg cccccggcgt agctatcgca cccggcggca aaccggtaat
    14041 ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata
    14101 acgctctccc atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa
    14161 acggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc
    14221 gataattgat gccatcgtca ccagctgatt tttatcttta taaggcagac cgtccgcacg
    14281 cccttcccag gcgctatcga ccgctttcac cattttcttg tgaactcgct tgagtaacgc
    14341 gacatcggtg gtattggcgg tatacatcaa ggtgtctggc cagaaccaac cttcaatcca
    14401 ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt
    14461 gtgcttgata tacggagcct cacgcaattg cttgaggtag tcgctcagac gcatcccttc
    14521 taccagtcgc agcgggaact gtgcttcttt accgctttcc aacaatttca gcatctcgcg
    14581 cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc
    14641 gatacgcagc agccattgaa aaacccgtgg gcgattgatg atcttatcag cataaagctg
    14701 ttcaccgagc gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat
    14761 aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc
    14821 cagtaccacc agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc
    14881 tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggtgac atcgccacag
    14941 gcacgtacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac
    15001 gcctcctcca gcgaggcttg cacttcgaca agctgataag aggattgtgc cagtaaacgg
    15061 atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact
    15121 acattgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg
    15181 tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcgaat caatacttgc
    15241 tcaagccgat taagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg
    15301 cttagcgcca acgtcatccc ttcgttacgc aaacggtcgt aatgtgcagg ataagccgta
    15361 acggagagaa tccgcgttgc tggtccgctg ttcaatgtgc tgtaccctcg cccgccacta
    15421 ccgcgactga tcacgacttt cagcacacca ttttgctgtt ctgctgccag cgttttcatc
    15481 tcttgttcaa gctgaggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt
    15541 agtcgcagga tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta
    15601 aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttataa
    15661 ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca
    15721 ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttatatg
    15781 ggtggaaaat gacaacttag atctttttaa agatcaaaga accattagtg ccaccgaagc
    15841 cgaaggagtt acacagcgtg tattccattc cgctgacctg acgtgcttca tgcggtacga
    15901 aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat
    15961 cgcgcagcgc caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac
    16021 cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt
    16081 tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt
    16141 agccaatctg actcgcttca atgcctgcat cacgcagagc atttgccatt gccagagctg
    16201 cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc
    16261 cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta
    16321 ccagcatacc ggcaccatcg cccagtacga agccatcacg ctctttatcc cacgggcggc
    16381 tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa
    16441 cgcccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat
    16501 acgcgataat acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga
    16561 tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca
    16621 caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg
    16681 tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc
    16741 gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga
    16801 caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct
    16861 cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat
    16921 ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg
    16981 tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt
    17041 ttgtcctcca aggagggaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc
    17101 gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc
    17161 ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc
    17221 cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga
    17281 agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac
    17341 gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt
    17401 tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga
    17461 ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta
    17521 ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat
    17581 gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc
    17641 caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg
    17701 cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa
    17761 gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt
    17821 actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat
    17881 ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg
    17941 cattaccgct tttgacagac ggaaaacaga tgaaaggtta gtttcgataa tatcgttcca
    18001 ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag
    18061 gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg
    18121 gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcac tgatcgcctg
    18181 agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc
    18241 agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa
    18301 attcatgatt ttcctctttt aaagctcgag cgccgctgcc atcgctgaag gttcgttcag
    18361 cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg
    18421 gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca
    18481 ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc
    18541 gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg tgattttcgc
    18601 taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct
    18661 cactggtaac ggcagcgcgc gttttgcgcc agcagcttta caggcagcgc cagcacgctc
    18721 aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga
    18781 aacgacctga ccttctgcag cttcttcaca cgctttcgca ataggcgcat catccagacc
    18841 gatgattgcc gccatagcgc ccgtgccttc cggtacggct tcttgcatga acttaccgcg
    18901 catctcaacc agacgcaccg catcagcgaa atcaatcacg ccagcgcaaa ccagcgcgga
    18961 gtattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca
    19021 tacgcgatac agcgcaacag atgcagtcaa cagcgctggc tgggtttgcc aggttttatt
    19081 cagttcttca gctgacccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga
    19141 agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc
    19201 aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt
    19261 atcctagaaa cgaaccagcg cagagcccca agtgaatcca ccgccaaagg cttcaagcag
    19321 aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac
    19381 agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatcgacat
    19441 accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca
    19501 gtccagttga gaacggtcaa gattattcgc cgccagcgtc tcatcaacga tgtgcgccag
    19561 ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc
    19621 tggattcacg cggtcggcgt ttggcagcgt cagcaattca ccataactac cgtcggcatg
    19681 cagatgggtg gaaatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc
    19741 atcgccaaaa ataataatag tcccacgatc tgttggatcg caggtgcgcg ccagtacatc
    19801 ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct
    19861 taatgcatag gtgaaacctg cacaggctgc tgcaacgtca aatgccgggc aacctttaat
    19921 gtccaacatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt
    19981 tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt
    20041 cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg
    20101 gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc
    20161 gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta
    20221 cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt
    20281 cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata
    20341 cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct
    20401 gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc
    20461 cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc
    20521 tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc
    20581 cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta
    20641 acgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact
    20701 cattggcttc aagatagccg atataattga tagaagggat tgttttaagc accgctgagg
    20761 catcccgaat actgtcaaga ccctttactt cttcttcacc aatattgagc aacgccacgc
    20821 gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca
    20881 ccaacattgt gctgtcacaa tcgacgttgg cccctaaatc gaggaccacc gttttgccct
    20941 tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga
    21001 gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg
    21061 ctcgaccttc tttcaccagc tccagggcca tacgcattga actcccacgg ctggcgcgga
    21121 tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac
    21181 gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga
    21241 ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca
    21301 cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat
    21361 cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat
    21421 cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt
    21481 gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc
    21541 ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg
    21601 tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa
    21661 ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca cttcttcagg
    21721 cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg
    21781 aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc
    21841 gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga
    21901 acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg
    21961 ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt
    22021 atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc
    22081 gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt
    22141 acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact
    22201 ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg
    22261 cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca
    22321 aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat
    22381 atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg
    22441 caaatctctt ttgaatgtgc agcacctgag gtcgacgaaa ccccacgcag cgacgaatca
    22501 ccgcgacagt tggtgcttcg actggcacaa gaaaaatcgc aatctctggc gtcacgttat
    22561 ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt
    22621 aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc
    22681 accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg
    22741 gagccttttg acgtccattt ccgtcaccta agcgaggcgg agattgataa ttacgtgcgt
    22801 aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg
    22861 tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct aatcgcgctg
    22921 tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt tgcgccggat
    22981 gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa acgccttatc
    23041 aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac gcagcttttg
    23101 caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct caccggtccc
    23161 cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc
    23221 ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg
    23281 acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg
    23341 caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc
    23401 ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc
    23461 ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg
    23521 cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctac
    23581 cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc
    23641 cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac
    23701 cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc
    23761 cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac
    23821 gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg
    23881 ttttttgttc acccgcactt cacctttacg caaaatacgg taaatcatac tttttggtac
    23941 gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat
    24001 agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac
    24061 aggattcgcg ccactcattt ttctatgctt atttttagtt ttgcacctta ttacttcact
    24121 acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg
    24181 agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa
    24241 tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat aacccatttt
    24301 gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc
    24361 gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt
    24421 ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg
    24481 cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt
    24541 tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt
    24601 aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc
    24661 ttgtagatgg gcagcgtctg tatgacctgg atatcgaaag tccagggcac gagcagaaaa
    24721 aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa gctgcttttg
    24781 ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt
    24841 tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc
    24901 aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca
    24961 cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg cgcgcaggtg
    25021 gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca ctggcaagcc
    25081 ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc aaatctgctg
    25141 aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc aaaaaagccg
    25201 ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcct
    25261 tccgcgatta tttacgtcag gatatcggcg aaatccttat cgataacccg aaagtcctcg
    25321 aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac
    25381 tgtacaccgg cgagatcccg ctgttcagcc actaccagat cgagtcgcag atcgagtccg
    25441 ccttccagcg tgaagtccgt ctgccatccg gtggttccat tgttatcgac agcaccgaag
    25501 cgttaacagc catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa
    25561 ctgcgtttaa caccaacctc gaagccgccg atgagattgc tcgtcagctg cgcctgcgtg
    25621 acctcggcgg cctgattgtt atcgacttca tcgacatgac gccagtacgc caccagcgtg
    25681 cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc
    25741 atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca tcactgggtg
    25801 aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat
    25861 cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag aacacccagg
    25921 aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa aaacgttctg
    25981 cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg ccaaacgatc
    26041 agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa acgccgacct
    26101 taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt
    26161 tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg ccggatgtac
    26221 cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca ccgaaagctg
    26281 caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc ttcggcgcac
    26341 tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca acaccgaaag
    26401 cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc aaccgccgtg
    26461 accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag
    26521 aaaaccgtcg taatcgtcgc caggcacagc agcaaactgc cgagacgcgt gagagccgtc
    26581 agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagtg ccgcgtcgtg
    26641 aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga
    26701 atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc
    26761 cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag
    26821 aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag
    26881 ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac
    26941 cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt
    27001 ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg
    27061 agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg
    27121 cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag
    27181 agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactgaggtc cctgtcgccg
    27241 ccgctgtcga acctgttgtt agcgcgccag ttgttgaaga aatggctgaa gtcgtagaag
    27301 cccccgtacc ggttgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga
    27361 ttgccgccgc ggtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc
    27421 aggaagtcgc agaacacgca gaaccgatgg ttgaaccgca ggaagagacg gcagatgcag
    27481 atattgaaga agttgccgaa actgctgagg ttgtggttgc tgaacctgaa gttgttgctc
    27541 aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt tgaaacggtg gccgcggttg
    27601 aaccagagat caccgttgag cataaccacg ctaccgcgcc aatgacgcgc gctccggcac
    27661 cggaatatgt tccggaggca ccgcgtcaca gtgactggca gcgccctact tttgccttcg
    27721 aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc gctcctgcgc
    27781 gtccgcaacc tgttgagtaa taattagctc aaagtaatca agccctggta actgccaggg
    27841 cttttttatt tcatgtttga atcaacatat taaaattcat caaatggcat ccttgctaac
    27901 caacaatgca aaataggcaa aaaaaatgcc cgccccggcg atcccagagc gggcagacaa
    27961 aacataccca gtttcatgat atgttttaaa gcgagcttat ttgttgagct ggaagagcga
    28021 caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt gctgcatgat
    28081 gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct gctgcgtttg
    28141 ccctaaagcg cgatcgctac ctaaggaatc cagcgactcc agtttgttca gctgcgtgcc
    28201 taattccgcg cgaacggtaa ggacattatt cagcgagttt ttcagtccac ggttggtttt
    28261 atccaatgcc gcagcggcga tttctttatc cgcttcgcta tccgcgaccg gcgttttcag
    28321 ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc taccgtctgg
    28381 ttccgctacc gcgttactgg taatactgtc gaaaattttg tcgcccgtat gtcctatcac
    28441 catcgaacgc gaagcatcga cctgttgctt aatactttct gttccgccaa cataatcacc
    28501 gttagcctca ctaaacggcg cagtctctgt tttatatgta gcgcccatta ccgtcagtgg
    28561 tgttcgccag attcagcaac tggtcacgaa gcccctgaat atccgtagcc agcgaggccc
    28621 ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat tttttcctga gcattctgga
    28681 tagcagtggt gacctggcta agtacactct cttccagtga cactttttga gtggcgaaag
    28741 tacgtgccag cgtgtactgg ctgttttgcg cctgtgcctg ggagagaaca acagcttgtg
    28801 atgcagcaat gggatcgtct gaagggttaa cgactcgctt acccgtcgac atctgctcgc
    28861 cgtacttcat ccattctgcc tgagaattgg tgatgccgcg catgttttgc tggtacatca
    28921 tctgggtact gaaacgcatt ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat
    28981 cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa tactgctgaa aacgttgcag
    29041 atttccgtac tcctcatcga gattgacacc ggaaatcgac tgctgctgat tggaaagctg
    29101 cgtcaccaca ttaccttgcg tggtgctact ggttttcaac gtcgcggttt tattaccgat
    29161 atcactcact aacgaggcat aagcgtcgtt aaaggatttc gcaccgccca ccgttttact
    29221 gttgctttgc agatccagca gggcctgacc gttgcggttg tcgctatcac ccgcatcttc
    29281 ttcgctcgcc atcgcgattt tcgcttcgtc ggtgattaat acatccatgt tgacgatggc
    29341 gtcacttact ggtttcagcg tgaagctgtc gttaacggca ggcgttcctg taaacgtcaa
    29401 ctccagacca tcaaatgcca ctttaccgtt ggcatctggt gtcgccgtaa aagtggtatt
    29461 gctggcaagg cgggtgacct gccactgatt attatcgaac gagattttgt aatctgtcgc
    29521 cagtaccacg gaggcatcag ttaccgtggc accgatcgca acgtcacctt tgtttttcgt
    29581 gttttgcaga accgcgggct taccgatagt aaagaaatct tcaccagcat cgccgttagc
    29641 atcaaatccg gctttgtgtt ggctgttgaa agcctcggca aatgccagcg ccagttgtcc
    29701 aagcgtatta cgcgtctggt ccagatcctg agaacggaat gtcagaatgc cgcccagcga
    29761 cccggtattc agtaatttct ccgggatctc aatattgcct gccgtcccat caacgtaagc
    29821 gacagtcgta cgagaagggt cggcgctgga aggaactgcc gccagttgcc gcgccgtact
    29881 tccctgaacc agtgagtaac cattggcatc gtgatgttat aagtaccgcc atcctgaacg
    29941 ctgacttcta cgccaacaat ctggtttaat tcgctcacca gttgatcgcg ttgatccagc
    30001 agattgttag gtgacgcccc tgcccccacg cctgtcaggc gcgagatttg atcgttcagg
    30061 ctggcaattt gtttagcgta attgttgatc tgatcaacgc tggcacctat cgcgatattg
    30121 acctgtttgt cctggtcgcg cagatattga tcggtggttt taaactgatt caccaaccct
    30181 tctgatttcc caatcagcgc ctggcgcgcc gccgggtctt ccgcgttact caccagcgtt
    30241 tgcaggctgg tgaagaaatc ctgcatctgt gttgccagcg aagaggtact ggtggagagc
    30301 atattgtcga ttttcgacat ctgctcatag cgggcggtca ggccgctact ttgcgtctgc
    30361 gcagcacgta actggttggt aataaacgta tcatactcac gctgcacacc agaaacgtag
    30421 acgccattgc caacccagcc gccagcgccc aacgtgctat tggcctgcgc cataatagtg
    30481 gtttggcggg tatatccggc aacgttatag ctggagatat tattacttgc cgtatttaac
    30541 gccgcctggg ccgcgttcag tccgctcatg gcgttattga tcaagctgga catggaggtt
    30601 ccttataagc cttcaattac agagtattat cggcagcgac ccgccggact tgagttattc
    30661 agaacagatt atcaatgttc atgctgtagg ttttgctcac cttgtcgctt atcgatttca
    30721 tctgctggat catgttggtg agtttacggg catagtgagg atcggtagca tagcccgcgt
    30781 cctgtaggac ctgcgccccc tgttccgcac tcgcggcggt cgtcacggcg gcgtagcgcg
    30841 ggttacgtgt taacagccca acgtaatccg acaatgcttc cagatacgag ctgtagaccc
    30901 gaaacttcgc ttttactttc ttcgcttcgc cgttttcata ttcagtcgtg gtgatttcag
    30961 tgacttgccc tttccagttg ccagaggctt tgacaccaaa caggttatag cttggctcgc
    31021 cgttttcgcg gcggatttgc cgttgccccc aaccagattc aagcgcagcc tgagcgagga
    31081 tcaaatgatg tggcacaccg ctttgctggc ttgccagttg ggcgggcagc gagagttgcg
    31141 cgaggaatgc tctactgtca cccggcagcg aatcatcgta gttacgtggt acggcctttt
    31201 gcaccagctg cgaaagcgtc tggttttgat aacgcaccac ggtttcgagc gggaatttca
    31261 tcggtgctgc tggcgtggac tcctctggca atggttgttc tggcgtcatc tgtttaacca
    31321 tcatctctgc aagccccaga cctttgcccg ccgtcatctg ttgggcaatt tgctggtcat
    31381 acatactggt atacagacga gtgtgctcgc tgctgaacag gccatctttt ggtaaagcgt
    31441 cgcgcatgct tttcaacatc atctgcacga acatcccttc cacctgacgg gccaccggac
    31501 ggatatttgc cgccggatct tcgctcgctt tcgcctttag ttcgttgagt gattgcgcat
    31561 cccaggccgc acttgccagt agtttgctgt cgctgatcat cagatgattt ccagttttgc
    31621 ccgcagacat cccgcacttt gcattgattg caaaatagac atcagatcca tcggcgtagc
    31681 gcccagcgca ttgagcgcac gcaccacgtt attgaggctg gcgctggaac gtacgctttg
    31741 cagcgaaccg ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacaa cagtctgtcc
    31801 accaccaaac ggtgtatctg gctggctgac attggcctga cgattaactg ttactgagag
    31861 atttccctgc gctaccgcgc agctgtcgag ggtcacttcg cgattcatca ccaccgaacc
    31921 ggtgcgcgag ttaatcacta ctttagcgtc ctgcggggtg acattaacct gcatattctg
    31981 gatatcggca aggaaacgga cctgggaact gttgccactt ggtacgcgca cctgaatagt
    32041 ccgcgcatct aacgcggtgg cgctgccata tccacgcacg cggttgatgg tgtcagcgat
    32101 ttgctgcgcc atgctgaaat cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc
    32161 aaactggctg ggcaattcac gttcaataac cgcaccattg gtgatccgtc caccgttcag
    32221 ttggttaacc tgaacactgc taccgcctgc ggaggctcct gcgccgccaa ccagaatatt
    32281 gccctgcgcc agcgcataca cctgactgtc aacgccctta agcggagtca tcaacaacgt
    32341 acctccacgc aagcttttgg cattacccat ggaagaaacc accacatcga tggtttgccc
    32401 ctgacgtcca aacggaggaa gtgacgctgt caccattacc gcagcgacgt tttttagctg
    32461 catattggtg cccgtcggaa cggtaattcc cagctgtgag agcatgttat taagcgtttg
    32521 tgtggtaaac ggcgtctggg ttgtctggtc accagtgcca tccagcccca ctaccaagcc
    32581 atagccaatc agtgagtttt gccttacccc ctgaacactg gtgagatcgc gaatacgctc
    32641 agcctgagcc gccgtcgtaa ccagtagaag aattaatgca gagagaaatt taatcaccac
    32701 agcctcactt acattggcga caggttaagg aagaaacgct gcaaccagcc catattttgc
    32761 gcttcgttaa tgtagccatt gcctacgtat tcaatacgcg catccgccac ctgagtagac
    32821 ggtacggtat tgctgccgct gatagtgcgt ggattaacca cgcccgagaa gcgaataaat
    32881 tcggtaccct gattaatggc aatctgtttt tcacccacca catgcaggtt gccgttgacc
    32941 agtacctggt caaccgtcac cgtcaacgtg ccgctaaagg tattgctggc attggccccg
    33001 ccctttccgt tgaacgtgtt accaccggag gcttcgacat cggcacgagc gttaccaaac
    33061 agcccctgca aatagcgcgg cacagtatca aagccaaaat tagttttacc gtcacggctg
    33121 gcattcgcag aggagctttt gctggcgctg acgttctcct gcaacacgat ggtcagcgta
    33181 tcgccaatat tgcgtggtcg acgatcttca aacagcggtt gatagccata gttaatcggc
    33241 tgagcagact ggaaaataga accgttggcg acgggcgtcg gaccgggaac cggttgtgca
    33301 ctggtcgccc cctgcaccag cggcgtggag ggtatccagg cgcagccggt tagtgaaagc
    33361 accaacaagc tggaaatggc ataagtatgc gcagcgtttt tttgcatggc tatcatcttc
    33421 aaaaatcagt aaaccggtga acctgccacc ggttatgcct tagagttgcg tcagtttttg
    33481 cagcatctga tcggtggtgg acaccgcttt actgttgatt tcgtaagcgc gttgcacctg
    33541 aatcatattg accagttctt ccgccacgtt gacgttagac gtttcaacat acccttgata
    33601 cagcagtccc gcgccgttca ggcccggcgt gctttcgttc ggtgcaccgg aggattgcgt
    33661 ttcggtgtag aggttttcgc caatgctctc cagtccggta tcattcataa aggtggtgag
    33721 attgagctgc ccaacctgaa ccggagctgc ctggccttgt tgggttacgc tgaccacgcc
    33781 atcacgaccg atggtgatac ttaacgcatt cgccggaatg gtgatcgctg gctgcacctg
    33841 aaaaccacca gccgtcacca gctgcccgtt ctgatccacc tggaaagagc cgtcacggat
    33901 ataggctgat gagccatctg gcaacattac ctggaaaaag ccctgccctt taatcgcgac
    33961 atctttgctg ttattggtct gcgacagatt gccctggctg tgtaagcgtt cagtggcgac
    34021 cgggcgtacc cccgtgccga tttgtaatcc ggagggtaag gtggtttgtt cggaagactg
    34081 tgcccccggc tggcgaatgg tttgataaag cagatcttca aacaccgcgc gctgacgctt
    34141 aaaaccgtta gtactgacgt ttgccaggtt gttggcaatg acgtccatat tggtttgctg
    34201 ggcgtcaagg cccgttttgg cgatccataa tgaactgatc atataatgtc ctgtagtgga
    34261 ttgctgacaa gcagagcaga agcacgcgac agtgtgaatt tcccctcacc ctaaccctct
    34321 ccccaaaggg gcgagggaat agtccgcgcg cttttattgc aatgtcatca atcgttcaat
    34381 taactcatcg acagcagttg gttggcacgg cctgcgttat catcgacgct gctgatcacc
    34441 ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt cgctcattgc cgcaacggca
    34501 ttgacgttac tgccttccag aacccccgac atcacacgca aggttggatc tgcctgcagt
    34561 accggccctc gcgtggcctg ggtttctgcg cttaaacgaa aaatgccgtc gtcaccgcgc
    34621 tgcacttcgc tgcccgtggc tttcaccagt ttcagacgcc ctactggcgc aactgtattt
    34681 gccggatcgc ccggattgag cgccgagatt gtgccatcgg cagcaatcgt gatttccgcc
    34741 ccttccggca cagcaattgg ccccgcctcg cctatcaccg gatgcccctg aattgtcagt
    34801 tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat acccttcgct gccgtcagcg
    34861 gtctgcacgg ccagccatcc tgctgcaacg caacgtccag cgggcgagag gtgtaatcca
    34921 tcttgccggg cgtcatgtct gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca
    34981 gtgaaagccc ttccaccggc actgcgcgca aggcgtttaa ctgcgcgcga aaaccgggcg
    35041 tagaggcatt ggccagatta ctggcggtta ccgcctgttg attcagcgtc tggctggctg
    35101 ctcccatcgc agtataaatt gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag
    35161 ttaacccgcg tgttgaggat ctggtcctgg gttttgatgg tctgggcgtt agacttatag
    35221 ttacgctggg caacgatcat attgaccagt tctttactga gatcgacgtt ggacgcttcc
    35281 agcgcaccgt tggtcagagt gccaaagttt cccgtcccgg ctgtccccaa cagcgccacg
    35341 ccagaagatt gcgtcgcaga ccagacgttg tcgccttcgg atgccagacc ttcgttgttg
    35401 gcaaagttcg ccagtacaat ctgccccagc agttgggttt gttcgttgga attgttgccg
    35461 acaaccgtac cgtcatcatt gatttgataa ctcaccagat cgcccggttt gtagccattc
    35521 tgggtggttg ccacaatgtt gttagcgccg gttgcgatat tattcgccat cgcaccatcc
    35581 actaatgtgc cattagcatt aaattccagt gttgtcgctg tcttcgcaat gctgtttgga
    35641 tcactgctat cctgggtgta gacctgccag ttattatccc cggtcttcac aaaatagacg
    35701 ctcatgtcat gagcattacc ctgactgtcg aaaacagtca ccgaaccttt tttgttatag
    35761 ctatccgcat tgctggcgct gaatggcgta acagtaggaa gcggatcact ggaattcagg
    35821 ttgatctgca tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt cgggatcgaa
    35881 atattggtcg gattcgcccc ttgctgaata gtcggcggcg taccggttac cgggtaaccc
    35941 gtcagctgta aaccttgcgt attcaacagg ttacggtttt catccagctt aaattgtccg
    36001 ttacggctgt agaacaccga accgttgctg tctaccagac ggaaaaaacc gttctggctg
    36061 atagcaacgt ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa gtcctgagtg
    36121 ataccggcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc ggcaaaagag
    36181 gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc aataacatcg
    36241 aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat gactgactcc
    36301 tgaaatgtga aggcttaaat tatctgccgt acttcgtcga gggtggtggt gccgtaagtg
    36361 ccgagatcca gcgtattacc gctgttgccg cggatcacac cctgcaccag agcaaactgc
    36421 agcggctggg caaccagttg tgtaccaccg ttactggcgc taatcgctac attgtaagaa
    36481 ccgttcggcg cagtgctgcc atcagtcaac gaaccgtccc aggtgaaact gtgaactccg
    36541 gcggtcagtt caccaatatc aatggtgcgc acaaccgcgc catttttatc ggtgatggtg
    36601 gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt cgtggtcaca
    36661 gccccttctt cactgccggt tcccgcaaga acagttgtgc cggggatcat cacgccgtga
    36721 ccgatcaggt tactggcctg taacgactgg ctgttatcaa tctgtccgga aatagatccg
    36781 agcgtggtat tgagtttttc aatcccgctg accgtgctga tttgtgccaa ttgcgacgtc
    36841 agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc caccagcaaa
    36901 gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact actgctggtg
    36961 gtactgacgc cggtatttgt cggatcggtg gtggttaccg caatggacat agctttctcc
    37021 tttattgacc gagcgtaagg gttttcagca tcatgctttt caccgtgttg agcacttcaa
    37081 cattggcctg atagctgcgt gacgccgaca tggtgttaac catctccccg acgacatcaa
    37141 cgttcggcat ttttatgtag ccctttgcat ctgccagcgg attacctggt tcataaacca
    37201 gtttgtccgg ggcctgactt tcttaacatc ggcaaccttt acgccgcctg tcgcagcacc
    37261 tggtgcagcg ttaacctgga ataccacctg ttttgcccga tatggctgtc catcaggacc
    37321 ggtcacgcta tcagcatttg ccagattact ggccgccacg ttcaggcgct gggactgggc
    37381 agttaacgcc gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct
    37441 ctgtaaaacg ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta
    37501 ttgcaggctg ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc
    37561 aagcgaaggc tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg
    37621 cgccgggatg tgctgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc
    37681 acgttgcatg acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg
    37741 ggtatcggca ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag
    37801 cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt
    37861 atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc
    37921 aaaatgggtc gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta
    37981 gaaatggctg ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact
    38041 gttcagtccg ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc
    38101 ccaactcgcg ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact
    38161 accgccatgc gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt
    38221 gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac
    38281 aggaaattat gtggttgccg cgatgcctat tgtgcgggga ggaaagctgg aagctggcaa
    38341 tgtcaaactg aaacgtggac gcctggatac cctgccaccg cgtacggtgc tggatatcaa
    38401 tcaacttgtt gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac
    38461 ccagtttcgc caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg
    38521 tgatgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa
    38581 tgcgcgggtg cgcatggtat cggaacaggt agtcagcggc gttgttgatg cagatgggaa
    38641 tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac
    38701 acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct
    38761 gaagcctgta agcaccgttc aaccgcgcga aaccaccgac gcgccggtaa cgaacagccg
    38821 ggcggcaaaa acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa
    38881 actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc
    38941 gattcgtaac ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga
    39001 agcgcagcaa gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc
    39061 agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct
    39121 ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc
    39181 tggcgacgct ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg
    39241 ttgatattag tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga
    39301 atcaacataa cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa
    39361 tgttgaaacc gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacacccc
    39421 atcgcggcag taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga
    39481 atgcattgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt
    39541 gcagcgaagt acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag
    39601 ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac
    39661 atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta
    39721 aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga
    39781 ccaatagaaa gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat
    39841 tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa
    39901 taaaagccag gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca
    39961 attaacgccc gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc
    40021 gaaacggtta acggacctga aagaatgccc aacgcaaccg cactcggcag tgccaacagg
    40081 aaacaaagac gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca
    40141 aaacttttcg acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag
    40201 ggaaactcca ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac
    40261 gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc
    40321 ggtcccatct gtttcaccac gcgcattgct ccagcatcgt ggaagttaat gcgcggcagg
    40381 accagcatgc cgatcttctt caggtgcggt agctgataca ccagctgcag aatgccgccg
    40441 accgtaacag cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc
    40501 gcgaaaccaa tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg
    40561 ttccacgtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga
    40621 aaggtaatct ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct
    40681 ggcgcggtca ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc
    40741 agcgcaagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc
    40801 tgcttacttt tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca
    40861 aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc
    40921 cctgcgccaa agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac
    40981 atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccgg
    41041 acccacgggc aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctagatt
    41101 atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat
    41161 cttgtcaacg atacgttgcg ccagcactgc ctattcgccg gcggtttgcg gaactgtctg
    41221 attttacacg cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt
    41281 gctctgccaa ccaggaatcg gtttattcac tacaccctgc ccgcgctcct cacgccattc
    41341 gcgcatatcc gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg
    41401 acttccggca cggcgatgca tacaagtggt gatctgcaaa ggaccggccg aaaaatggtg
    41461 ctcggcaaac agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc
    41521 tttgccgccc gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt
    41581 gaaataaaga tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttagcgaggc
    41641 tgcggtggcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc
    41701 gaccatcaac gtcagttttt tccgcgccgc cagttccacc agcctttcag catcgcgcag
    41761 attttctgcc agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac
    41821 cacgtcaaag tggctggcgg tgctggagtg cacaaaaacc gcatcacaac tggcggcaag
    41881 gctggataac gaatcggcat aaggaatgcg ccagctgtca caaattggca gggctttggc
    41941 gcgcgtaggc gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa
    42001 ccacgctttt tgcgcaatgc cgcctaatcc cactacgccg atacgtaatt ttttcacttt
    42061 aatctcccag atgggccagc aacgaatcaa gacgttgttt cagttctgcc acttcgattt
    42121 ccagggcttc gacgcgggtc tgtaaatcac cgtcaaccat gttcgacata gccgttaccg
    42181 ccggctgatc ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg
    42241 gttcgcgggc cagacgcacc acaaaaggac catcttcgcg attcgccagt tgttccagcg
    42301 tcgactccac ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt
    42361 cacccggcgt ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc
    42421 tcagtttcag atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat
    42481 taccaaaacc gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct
    42541 gcacttcgga ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg
    42601 tgactacacc attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca
    42661 agcaaccaat tacgcgagct tccagggcag taagttgata tttcatgaga ttctccttag
    42721 cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca
    42781 tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa
    42841 tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg
    42901 cgctgcatat aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat
    42961 ttttgcccaa tcgaataacc gagatagcag gcatgaaaag agccgcgaac aacattggaa
    43021 aaattggcaa cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct
    43081 gaaccttgtt tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa
    43141 cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa
    43201 taatccgcca gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg
    43261 gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc
    43321 gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag
    43381 tccaaaatgc cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac
    43441 gttttgataa aaaaatactg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa
    43501 tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg
    43561 aacctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt
    43621 gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc
    43681 ggtattgctc tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca
    43741 attgccgacc gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc
    43801 ggattcgcca caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc
    43861 tcgggactcg gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc
    43921 cgtccacaac aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccagtgcg
    43981 gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc
    44041 acaggggccg ttttgtttgt gctatgtgcg gcgttcaatg cgtggttgtt accggcatgg
    44101 aaactctcca ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag
    44161 cgttttgtca cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg
    44221 ctgatgctgc caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg
    44281 tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa
    44341 aagcattttc gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg
    44401 atgccggtgg gcatggtcag cggcctacaa caacttttca ccctgatttg tctgttttat
    44461 atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca
    44521 agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt
    44581 ggttatatcg gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt
    44641 ccgtggatga tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt
    44701 agtcagaaac gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga tactcccccc
    44761 tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac
    44821 atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc
    44881 cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt
    44941 caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat
    45001 atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct
    45061 gccattgaag agtggctgcg caaggtcaat ggctacgtca acaaactgct gttgccgcgt
    45121 tttgccaaat cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac
    45181 aagaaagagg ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt
    45241 aagaatatca gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg
    45301 aatggcgaac tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg
    45361 gtagccggaa tcaactggcc aagccgcgtt gctaattacc gcgataatat ggcgaaacag
    45421 acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt
    45481 attcgcgggt tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca
    45541 tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt
    45601 tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca
    45661 atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa
    45721 agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca
    45781 aattggtcgc gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa
    45841 ctccctgttc ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt
    45901 tgataaagaa aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca
    45961 accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg
    46021 caattacttt aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc
    46081 aatggcgaaa aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt
    46141 tacggattaa tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt
    46201 ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac
    46261 tgcaccatcc caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga
    46321 tggcgacatg ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt
    46381 aatgcccaat ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg
    46441 tattcttgac gccgtacctg ccgggcacga tttcacccca ttgatgacct gttatttaac
    46501 agattcgctg gatcctaatg agctggagcg cggatttaat gaagccgtgt tcaccgctgc
    46561 aaaactttac ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc
    46621 aatcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga
    46681 agtgacacat gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat
    46741 ggaacctctg cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa
    46801 agatgctgcc gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca
    46861 gcatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg
    46921 tttacccatc ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg
    46981 ttttaatcga gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag
    47041 cagttgcggc tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt
    47101 ctttgaagag atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca
    47161 gttctatggc ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt
    47221 tgctgaaagc atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt
    47281 acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct
    47341 gtataaataa ccagtatatt caacaggggg ctattatgcg aattgaagtc accatagcga
    47401 aaacttctcc attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta
    47461 ttcagtatgc gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata
    47521 atttatcggt tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg
    47581 aaacgtggga aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt
    47641 ttgccggatc gccccggctt ttttatttta cttacgaatt ttgagtacaa aatttctaca
    47701 cttcctcaaa ataattgcac aatatgctaa aaaaatatga atgacatgct gttttattgt
    47761 tcatataaat cttaaaagca gaaatttatc tgttcccccc tttaaaaatt cggtgaatac
    47821 ccttacttat tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg
    47881 ttgtctagta acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact
    47941 catccgctcg tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt
    48001 ttgcactacc agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc
    48061 tggttaacca ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa
    48121 attgaatccg gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc
    48181 cacccaaaag gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt
    48241 taattactct gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt
    48301 attggcagcg gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac
    48361 gtgctaatga ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg
    48421 cgattaattc gccatgctta tggtgaaggc gaaaagtatg tcccgttggt cctccgcgcg
    48481 caaacgctgt gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct
    48541 ggtgtcatta acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc
    48601 gaacagtggc aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca
    48661 gaaatacgcg tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc
    48721 agcgaactgg cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg
    48781 ttcaactgcc cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggtt
    48841 gacggtgagt atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg
    48901 ctcccggagc tgcctgtcca gcccgtacgt aaagtatttg cctggtatca ggccgatggc
    48961 cgctatagcg tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa
    49021 tattatggtt ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag
    49081 gttatccatt cagcggatga acgtgttccg tttgcggaag tggccagtga tgggtcagaa
    49141 gccttcccgt tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc
    49201 tgcacctatg ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat
    49261 aatacactgc tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg
    49321 gaaatagctg ccgattttgc gcaagaccaa aaaagcgatt ttgatttgac gccattcagg
    49381 ctttcccgct tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt
    49441 atttaatgcg tcggctgttg cactatctca tcaataatat tcgcgagcat ctgatgcttt
    49501 atctttttct ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt
    49561 tttccgcact gacataaact gacaaatatt taatctccct ttcatttagc gtgaaaaatt
    49621 gaatgagatt attcctgctt ctacagcact caccagacat ctctgatgct ga
//
LOCUS       DAZOZX010000015        49534 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZOZX010000015 DAZOZX010000000
VERSION     DAZOZX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.015"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1152)
                     /gene="setB"
                     /locus_tag="ACHUPT_000828"
     CDS             complement(<1..1152)
                     /gene="setB"
                     /locus_tag="ACHUPT_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0818521.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1520..2650
                     /gene="fruB"
                     /locus_tag="ACHUPT_000829"
     CDS             1520..2650
                     /gene="fruB"
                     /locus_tag="ACHUPT_000829"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0818522.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2650..3588
                     /gene="fruK"
                     /locus_tag="ACHUPT_000830"
     CDS             2650..3588
                     /gene="fruK"
                     /locus_tag="ACHUPT_000830"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0818523.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3605..5296
                     /gene="fruA"
                     /locus_tag="ACHUPT_000831"
     CDS             3605..5296
                     /gene="fruA"
                     /locus_tag="ACHUPT_000831"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0818524.1"
                     /translation="MKTLLIIDANLGQARAYMVKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5720..6661
                     /locus_tag="ACHUPT_000832"
     CDS             5720..6661
                     /locus_tag="ACHUPT_000832"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0818525.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6649..7587
                     /gene="psuG"
                     /locus_tag="ACHUPT_000833"
     CDS             6649..7587
                     /gene="psuG"
                     /locus_tag="ACHUPT_000833"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0818526.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7681..8931
                     /locus_tag="ACHUPT_000834"
     CDS             7681..8931
                     /locus_tag="ACHUPT_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0818527.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9002..9696)
                     /locus_tag="ACHUPT_000835"
                     /pseudo
     CDS             complement(9002..9696)
                     /locus_tag="ACHUPT_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9827..10768
                     /gene="rihB"
                     /locus_tag="ACHUPT_000836"
     CDS             9827..10768
                     /gene="rihB"
                     /locus_tag="ACHUPT_000836"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0818528.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10868..12118
                     /locus_tag="ACHUPT_000837"
     CDS             10868..12118
                     /locus_tag="ACHUPT_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0818529.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12174..13262)
                     /locus_tag="ACHUPT_000838"
     CDS             complement(12174..13262)
                     /locus_tag="ACHUPT_000838"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0818530.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13265..14122)
                     /gene="nfo"
                     /locus_tag="ACHUPT_000839"
     CDS             complement(13265..14122)
                     /gene="nfo"
                     /locus_tag="ACHUPT_000839"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0818531.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14196..15245)
                     /locus_tag="ACHUPT_000840"
     CDS             complement(14196..15245)
                     /locus_tag="ACHUPT_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0818532.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15344..16225
                     /gene="yieE"
                     /locus_tag="ACHUPT_000841"
     CDS             15344..16225
                     /gene="yieE"
                     /locus_tag="ACHUPT_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0818533.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16431..17900
                     /gene="lysP"
                     /locus_tag="ACHUPT_000842"
     CDS             16431..17900
                     /gene="lysP"
                     /locus_tag="ACHUPT_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0818534.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18192..20183
                     /gene="cirA"
                     /locus_tag="ACHUPT_000843"
                     /pseudo
     CDS             18192..20183
                     /gene="cirA"
                     /locus_tag="ACHUPT_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20215..21050)
                     /gene="yeiG"
                     /locus_tag="ACHUPT_000844"
                     /pseudo
     CDS             complement(20215..21050)
                     /gene="yeiG"
                     /locus_tag="ACHUPT_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21308..21976
                     /gene="folE"
                     /locus_tag="ACHUPT_000845"
     CDS             21308..21976
                     /gene="folE"
                     /locus_tag="ACHUPT_000845"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0818535.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21993..23069
                     /gene="yeiB"
                     /locus_tag="ACHUPT_000846"
     CDS             21993..23069
                     /gene="yeiB"
                     /locus_tag="ACHUPT_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0818536.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23211..24251
                     /gene="galS"
                     /locus_tag="ACHUPT_000847"
     CDS             23211..24251
                     /gene="galS"
                     /locus_tag="ACHUPT_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0818537.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24531..25529
                     /gene="mglB"
                     /locus_tag="ACHUPT_000848"
     CDS             24531..25529
                     /gene="mglB"
                     /locus_tag="ACHUPT_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0818538.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25590..27110
                     /gene="mglA"
                     /locus_tag="ACHUPT_000849"
     CDS             25590..27110
                     /gene="mglA"
                     /locus_tag="ACHUPT_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0818539.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27126..28136
                     /gene="mglC"
                     /locus_tag="ACHUPT_000850"
     CDS             27126..28136
                     /gene="mglC"
                     /locus_tag="ACHUPT_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0818540.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28172..29385)
                     /gene="preA"
                     /locus_tag="ACHUPT_000851"
                     /pseudo
     CDS             complement(28172..29385)
                     /gene="preA"
                     /locus_tag="ACHUPT_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29379..30617)
                     /gene="preT"
                     /locus_tag="ACHUPT_000852"
     CDS             complement(29379..30617)
                     /gene="preT"
                     /locus_tag="ACHUPT_000852"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR0818541.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30811..31050)
                     /locus_tag="ACHUPT_000853"
     CDS             complement(30811..31050)
                     /locus_tag="ACHUPT_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0818542.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31053..31772)
                     /gene="sanA"
                     /locus_tag="ACHUPT_000854"
     CDS             complement(31053..31772)
                     /gene="sanA"
                     /locus_tag="ACHUPT_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0818543.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31922..32806)
                     /gene="cdd"
                     /locus_tag="ACHUPT_000855"
     CDS             complement(31922..32806)
                     /gene="cdd"
                     /locus_tag="ACHUPT_000855"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0818544.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32936..33631)
                     /locus_tag="ACHUPT_000856"
     CDS             complement(32936..33631)
                     /locus_tag="ACHUPT_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0818545.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33628..34026)
                     /locus_tag="ACHUPT_000857"
     CDS             complement(33628..34026)
                     /locus_tag="ACHUPT_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0818546.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34265..35212
                     /gene="dusC"
                     /locus_tag="ACHUPT_000858"
     CDS             34265..35212
                     /gene="dusC"
                     /locus_tag="ACHUPT_000858"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0818547.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35583..35666)
                     /gene="yohP"
                     /locus_tag="ACHUPT_000859"
     CDS             complement(35583..35666)
                     /gene="yohP"
                     /locus_tag="ACHUPT_000859"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR0818548.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35890..37326
                     /gene="mdtQ"
                     /locus_tag="ACHUPT_000860"
                     /pseudo
     CDS             35890..37326
                     /gene="mdtQ"
                     /locus_tag="ACHUPT_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37394..38140
                     /locus_tag="ACHUPT_000861"
                     /pseudo
     CDS             37394..38140
                     /locus_tag="ACHUPT_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38257..38835)
                     /locus_tag="ACHUPT_000862"
     CDS             complement(38257..38835)
                     /locus_tag="ACHUPT_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0818549.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39005..39592
                     /locus_tag="ACHUPT_000863"
     CDS             39005..39592
                     /locus_tag="ACHUPT_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0818550.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39766..40698
                     /gene="pbpG"
                     /locus_tag="ACHUPT_000864"
     CDS             39766..40698
                     /gene="pbpG"
                     /locus_tag="ACHUPT_000864"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0818551.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40736..42451)
                     /gene="dld"
                     /locus_tag="ACHUPT_000865"
     CDS             complement(40736..42451)
                     /gene="dld"
                     /locus_tag="ACHUPT_000865"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0818552.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42647..44944
                     /gene="bglX"
                     /locus_tag="ACHUPT_000866"
     CDS             42647..44944
                     /gene="bglX"
                     /locus_tag="ACHUPT_000866"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0818553.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45196..46113
                     /gene="osmF"
                     /locus_tag="ACHUPT_000867"
     CDS             45196..46113
                     /gene="osmF"
                     /locus_tag="ACHUPT_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0818554.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46120..47277
                     /gene="yehY"
                     /locus_tag="ACHUPT_000868"
     CDS             46120..47277
                     /gene="yehY"
                     /locus_tag="ACHUPT_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR0818555.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47270..48196
                     /gene="yehX"
                     /locus_tag="ACHUPT_000869"
     CDS             47270..48196
                     /gene="yehX"
                     /locus_tag="ACHUPT_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0818556.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48201..48932
                     /gene="yehW"
                     /locus_tag="ACHUPT_000870"
     CDS             48201..48932
                     /gene="yehW"
                     /locus_tag="ACHUPT_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0818557.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48913..49020)
                     /locus_tag="ACHUPT_000871"
     CDS             complement(48913..49020)
                     /locus_tag="ACHUPT_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0818558.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49080..49534)
                     /locus_tag="ACHUPT_000872"
                     /pseudo
     CDS             complement(49080..49534)
                     /locus_tag="ACHUPT_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11956 a        12204 c        13311 g        12063 t
ORIGIN      
        1 cagagtggcg ataatcatca ccatcgcaaa ccagaacaca gcgtgataat tccagatctc
       61 ggcgacgatg cccgccactg atcctgcgat gatccagccc acgcgcgacg tgttggtata
      121 gagcgtggtg gctgaacctg cctgaccggg catcaaatcc tgaaaataga gcatcccgat
      181 gccgcccaga atgccaataa aaatagcatt tagcagctgc aagcccaaca gaatggcagg
      241 tgaatgcgcc atcagcattc ctgcgtaaaa acagacgcca cccacggcag caacgcgcat
      301 taagaaacgc ttacccagac gtttggcgaa atatccggca atcaacatcg tcgggatttc
      361 cagcccggcg gcggtcccca tcatcacacc ggccagtttc tcgggaagat gcagttcgtt
      421 gataataaat agcggcatgt tgatgatgta caggctgttc gagccccaca tcaatgtaca
      481 aatgacaaac agcagtagcg tatcgcgacg gttacggcgc ggcgcttcaa ccgtgccggt
      541 cgccagcgga agctcttttt gcatcgacgg taaaaacagc cacaccatca cgccgcaaac
      601 aataaacgct accgctgcgc tcagatacat taccgtaaag ctgaaaccca tcgctaacgc
      661 ataagccagc ggtgggccaa tgacccatgc cagtgaaacc tgagcgcgta aaaaagagct
      721 gaacatcacc gcctcacgtc cggttttgtc ggcatgttca cgggcaaggg caaacatttg
      781 cgggttagcg gtcgagccaa agctgctaag aaagacgcca acgaatagca aaacaaagta
      841 gttgcgattc caagcaaaaa gggtgcaggc cagcacgcct aacaggcagc aaaagacaat
      901 cagcgatttg cgatcgccgc gcttatcaga gcgcccagcg agaaactgac ttaccagaat
      961 cccaatgaca gcgctgccgg taaagaagaa tcccaccatc gccggacggg catgtacttc
     1021 atcggtaagg aaaatactga gtgtcggggt ttgcagagca cccgcaatac cggtgagaaa
     1081 ggcaacgatt aaaaacgccg tcgaggtcag gtcaaacgat ttcgcgctgg agactgcggg
     1141 ggagttatgc atgctggtcg atcactggaa gaggaagacg cggagtttac gctgcttgtc
     1201 agaaaataaa cagacagcga aatcaaaagc gccacgaata atcaaaatgc catttcactg
     1261 ttgctgattg gctgctgacg ctgctgaaat gaaattgctg ataacggatt ttcccatcag
     1321 caattaggaa aaatgacaaa aaattgtgca gcacatcaaa cttttgctca taactttacg
     1381 gctttccttg cgtgctgaaa cagaagtatt atgctttctt gaaacgtttc agcgcgatct
     1441 tgtctttaac cctaagctag gttggcgctt tttttctcat agaggctgaa tcgtttcaat
     1501 tcagcaagag aggagaacta tgttccagtt atccgtacag gacatccatc cgggcgaaaa
     1561 ggccggagac aaagaagagg cgattcgcca ggtcgctgcg gcgctggtgc aggccggtaa
     1621 tgtagcagaa ggctacgtca atggcatgct ggcgcgcgaa cagcaaacct caacgttcct
     1681 cggcaatggt attgctattc cacacggcac taccgacacc cgcgatcagg tgctgaaaac
     1741 cggcgttcag gtatttcagt tcccggaagg cgtcacctgg ggtgacggtc aggtagcgta
     1801 cgtggcaatc ggtattgctg ccagctcgga taaacatctg ggcctgctac gccagctgac
     1861 ccacgtactg agcgatgatt ccgttgctga acaactgaag tcagcaacaa cagcagaaga
     1921 acttcgcgca ttactgatgg gcgaaaagca gagtgagcag ctgaagctcg acaacgaaat
     1981 gctgacgctg gatatcgtcg ccagcgatct gctgactctt caggcgctga acgctgcgcg
     2041 tctgaaagag gcgagggcag ttgacgccac tttcgtcacc aaagccatca atgaacaacc
     2101 gctgaatctc ggacagggta tctggctgag cgatagcgcc gaaggcaatc tgcgtagtgc
     2161 gattgcggta agccgtgcgg caaatgcttt tgatgtggac ggcgaaacgg cagccatgct
     2221 ggtgagtgtg gcgatgaatg acgatcagcc catcgcggtt cttaagcgtc tcgctgattt
     2281 gttgctcgac aataaagctg accgcttgct gaaagcggat gcggcaacgt tgctggcgct
     2341 gctgaccagc gatgatgcgc cgaccgacga cgtgttaagc gcggagtttg tggtgcgcaa
     2401 tgaacacggc ctgcatgctc gtccaggtac catgctggtc aataccatta aacaatttaa
     2461 cagtgatatt accgtgacaa accttgatgg caccggcaaa ccggcaaacg gacgtagtct
     2521 gatgaaagtt gtggcacttg gcgttaagaa aggtcatcgc ctacgcttta ccgcccaggg
     2581 tgcagatgct gaacaggcgc tgaaagccat tggcgacgct atcgctgctg gtcttgggga
     2641 gggcgcataa tgagcagacg tgttgctacg atcaccctta atccggctta tgaccttgtt
     2701 ggtttctgcc cggaaattga acgcggcgaa gtgaacctgg tgaaaaccac cggtctgcat
     2761 gcggcgggta aaggcatcaa cgtggccaaa gtattaaaag acctgggaat tgatgtcacc
     2821 gttggcggct tcctgggtaa agacaatcag gatggttttc agcaactgtt cagcgagctg
     2881 ggcattgcca accgtttcca ggttgtacag gggcgcaccc gaattaacgt taagctgacg
     2941 gaaaaagacg gcgaagtgac cgacttcaac ttctcgggtt tcgaagtcac ccccgccgac
     3001 tgggaacgct ttgtgactga ttctctgagc tggctcggtc agttcgatat ggtctgtgtc
     3061 agcggaagct taccgtcagg cgtcagcccg gaagcgttca ccgactggat gactcgcctg
     3121 cgtagtcagt gtccttgcat tatctttgat agtagccgtg aagcgttagt agcaggtttg
     3181 aaagcggcac cgtggctggt gaaacctaac cgccgcgagt tggaaatctg ggcaggccgt
     3241 aaactgcctg aaatgaaaga tgtgattgaa gctgcacatg cgctacgtga acaaggtatt
     3301 gcgcatgttg ttatttcact gggtgccgaa ggcgcgcttt gggttaatgc ctccggcgaa
     3361 tggatcgcta aaccaccgtc agtcgatgtc gtaagcaccg ttggcgcagg ggattctatg
     3421 gttggtggcc tgatttatgg cttgctgatg cgtgaatcca gtgaacacac actgcgtctg
     3481 gcgacagctg ttgcagccct ggcggtaagt caaagcaatg tgggtattac cgatcgtccg
     3541 cagttggccg caatgatggc gcgcgtcgac ttacaacctt ttaactgaca gcaggagagg
     3601 cataatgaaa acgctgctga ttattgacgc taatctcggt caggcacgcg cctatatggt
     3661 gaagaccctg ctgggcgcgg cggcgcgaaa agcaaaactg gaaatcatcg acaatccgaa
     3721 cgacgcagaa atggcgattg ttctcggtga ttccatcccg aatgacagcg cgctgaacgg
     3781 taaaaatgtc tggctgggtg atatttcccg ggcagttgcg caccctgagc tgttcctgag
     3841 tgaagccaaa ggccatgcga aaccttacac tgcgccggtc actgcgacag caccggttgc
     3901 cgccagcggt ccgaaacgcg tagttgcggt gactgcttgc ccgactggcg tagcacacac
     3961 ctttatggcg gctgaagcca ttgaaaccga agcgaaaaaa cgtggctggt gggtgaaagt
     4021 tgaaacccgt ggttctgttg gcgcgggtaa tgcaatcact cccgaggaag tagccgcagc
     4081 ggatctggtg attgtggcgg cagatatcga agtggatctg gcgaaatttg ctggtaaacc
     4141 gatgtatcgt acctctaccg gtctggcgct gaagaaaacc tcgcaggaac tggataaagc
     4201 ggttgctgaa gcaacgccgt atgaaccggc gggcaaagct caaacggcga ccactgaagg
     4261 taagaaagag agtgcaggcg cttatcgtca cttgctaacg ggcgtctctt acatgctgcc
     4321 gatggtcgtt gctggtggtc tgtgtatcgc gctttctttt gcttttggta tcgaagcgtt
     4381 taaagagccg ggtacgttgg cagcggcgct gatgcagatt ggtggtggtt cagcctttgc
     4441 gctgatggtg ccggtactgg caggttatat tgccttctcc attgccgatc gtccgggcct
     4501 cacgccgggt ctgattggcg gtatgctggc ggtcagcacc ggttctggct tcattggcgg
     4561 tattattgcg ggcttcctgg ctggttacat tgcgaagtta atcagtacgc aactgaaact
     4621 gccgcagagt atggaggcgc tgaaaccgat cctgatcatt ccgctaattt ccagtctggt
     4681 ggtcggtctg gcgatgatct acctgatcgg taaaccagtt gctggcattc tcgaagggct
     4741 gactcactgg ctgcagacca tggggactgc gaatgcggtt ctgctggggg cgatcctcgg
     4801 tggcatgatg tgtactgaca tgggcggtcc ggtaaacaaa gcagcgtacg cattcggtgt
     4861 gggtctgctg agtactcaaa cctatggccc gatggcggcg attatggcgg caggtatggt
     4921 gccaccgctg gcaatgggcc tggcaacaat ggtggcgcgt cgcaaattcg acaaagcgca
     4981 gcaggaaggt ggcaaagccg ctctggtatt gggactgtgc ttcatttcgg aaggtgcaat
     5041 tccgtttgct gctcgtgatc cgatgcgtgt gctgccgtgc tgtatcgtgg gtggggcgct
     5101 gactggcgca atctcaatgg cgattggtgc gaaactgatg gcaccgcacg gtggtctgtt
     5161 tgttctgctg atccctggcg ctattacgcc ggtattgggt tacctggtag caattattgc
     5221 cggtacgctg gtggcgggtt tggcctatgc cttcctgaaa cgtccggaag tggacgcagt
     5281 agcgaaagca gcgtaataaa aggtgtgtta cagggcagaa atttcctgcc ctgtaacatt
     5341 tgatgagcgc aaagagaatg accagcttgt gaatgtaatt ttatgaattt aaatggtttt
     5401 atctttcagt agaaacttca aacgttcatc aaacactaag gttatttcct caagggcaga
     5461 ttattttctg ccctttttta gtgttcctgt tctgtgcatc ccgtcacaaa ttcctcgctt
     5521 ttgtaattca ggcatttaat cgcattttgt gagcctgctc aaatctgccc attttgcctc
     5581 tctggcggta atttacttta cgtaaaacat gcatactatg agcacatgtt taaatatgaa
     5641 cacatgttta aaccgtcttt gcagtatcta tattcttgtg gattgacagg gttggtaatt
     5701 ttttaccaga ggacgtgcta tgcgcgaaaa ggattatgtc gtaattatag gttcggcgaa
     5761 tattgatgtc gccggatatt cacatgaatc attaaattat gcggattcaa atccaggtaa
     5821 aataaaattt acgcctggtg gagtagggcg caatattgca caaaacctgg cgttgctggg
     5881 taacaaagcc tggctactga gcgccgtagg cagtgatttt tatggtcaat cgctgctaac
     5941 gcaaaccaat caatctggtg tttatgtcga taaatgcctg attgtgccgg gagaaaatac
     6001 gtcgagttat ttatcattac tcgataatac cggtgaaatg ctggttgcta taaatgacat
     6061 gaatattagc aacgctatta cagctgaata tctcgcacag caccgtgaat ttattcagag
     6121 ggcaaaggtc attgtcgcgg actgtaatat cagtgaagag gcactggcat ggattctgga
     6181 taatgccgcc aacgtacccg tatttgtcga tccggtttca gcgtggaaat gtgtcaaagt
     6241 gcgcgaccgt ctaaatcaga accacactct gaagccaaac cgccttgaag cggaaaccct
     6301 gagtgggatt gcgctgtcag ggcgtgacga tgtggcaaaa gttgctgcct ggttccatca
     6361 acatggcctg aaccgactgg tattgagcat gggcggcgac ggcgtttatt acagcgatat
     6421 cagcggtgaa aatggctggt ctgcgccgat caaaaccaat gttattaatg ttaccggagc
     6481 gggcgatgcc atgatggcgg gacttgcttc gtgttgggta gacggaatgc cgtttgccga
     6541 atctgttcgt ttcgcacagg gatgttcgtc aatggcgctc tcctgtgaat acaccaataa
     6601 ccccgattta tcgattgcca acgttatatc gttagtggag aacgcagaat gtctgaatta
     6661 aaaatttccc ctgaattatt acaaatttct ccggaagtgc aggacgcttt aaaaaacaaa
     6721 aaaccggttg tggcgctgga atcgaccatt atttctcacg ggatgccgtt cccacaaaat
     6781 gcccagaccg caattgaagt agaagaaact attcgtaaac agggcgcagt acctgccact
     6841 atcgccatta ttggcggcgt gatgaaagtg ggtttaagca aagaaaaaat tgaattactg
     6901 ggtcgtgaag ggcataacgt gaccaaagtt agtcgtcgcg atttaccttt tgttgttgcc
     6961 gccggaaaaa atggcgcaac cactgtggct tcaacgatga ttattgcggc gcttgccgga
     7021 attaaagtat ttgccaccgg gggaattggt ggtgtgcatc gcggggcgga acataccttc
     7081 gatatttctg ccgatttgca agaactggca aatactaatg tcaccgttgt ttgtgccggg
     7141 gcgaaatcta ttctcgattt aggattaacc actgagtatt tagaaacctt cggtgtgccg
     7201 ttaattggct atcagactaa agcgctgcct gcgtttttct gccgtaccag ctcgtttgac
     7261 gtcagcattc gtctcgacag cgccagtgaa attgcccgtg caatggcggt gaaatggcaa
     7321 agcgggctga acggtggcct cgtggtagcg aacccgatcc cggaacagtt tgcgatgccg
     7381 gaggaatcta tcaatgcagc catagatcaa gccgtcgccg aagccgaaga gcagggcgtt
     7441 attggtaaag aaagtacacc gttcctgctg gcgcgcgttg ctgagctgac cagcggtgac
     7501 agcctgaaat ccaatatcca gctggtgttc aacaacgcca ttctggcgag tgaaattgcc
     7561 aaagaatacc agcgtctcgc gggttaattt ctgacgggca gaaagccgtc gcccgtactc
     7621 atcaagcata ccaaaacctg gcgctgacgc cgggtttact ggcatgaagg gaaaatcatt
     7681 atggatataa tgagaagtgt tgtggggatg gtggtgttac tggcaatagc atttctgttg
     7741 tcagtgaata aaaagagcat cagtttgcgc acggttggag ccgcactgct gctgcaaatc
     7801 gctattggtg gcatcatgct ctacttccca ccgggaaaat gggcagtaga acaggcggca
     7861 ttaggcgttc ataaagtgat gtcttacagt gatgccggta gcgccttcat ttttggttcg
     7921 ctggttgggc cgaaaatgga tgtcctgttt gacggtgcgg gttttatctt cgcctttcgc
     7981 gtacttccgg cgattatttt cgttactgcg ctcatcagtc tgctgtacta cattggcgtg
     8041 atggggctgc tgattcgcat ccttggcagc attttccaga aagccctcaa catcagcaaa
     8101 atcgaatctt ttgttgcggt taccactatt ttcctcgggc aaaatgagat cccggcgatc
     8161 gttaaaccgt ttatcgatcg cgtgaatcgc aacgagttgt ttaccgcaat ttgtagcggg
     8221 atggcgtcca ttgctggttc ggtgatgatt ggttatgccg gaatgggcgt accaattgac
     8281 tacctgttag cggcatcgct gatggcgatc cccggcggta ttttgtttgc acgtattctt
     8341 agcccggcaa ccgagccttc gcaggtcaca tttgaaaatc tgtcgttcag cgaaacgccg
     8401 ccaaaaagct ttatcgaagc ggcggcgagc ggtgcgatga ccgggctgaa aatcgccgct
     8461 ggtgtagcga cggtggtaat ggcgtttgtc gcaattattg cgctgatcaa cggcattatc
     8521 ggcggaattg gcggctggtt tggtttcgcc aatgcctctc tggaaagtat ttttggctat
     8581 gtgctggcac cgctggcgtg gatcatgggt gtggactgga gtgatgccaa ccttgctggt
     8641 agcctgattg ggcagaaact ggcgattaac gaattcgtcg cttacctgag tttctcccca
     8701 tacctgcaaa cgggcggcac gctggaagtg aaaaccgtgg cgattatatc ctttgcgctt
     8761 tgtggttttg ctaactttgg ttctatcggt gttgtcgttg gcgcattttc ggctatttcg
     8821 ccaaaacgtg ctccagaaat cgcccagctt ggtttacggg cgctggcagc agcaacgctt
     8881 tccaacctga tgagtgcgac tattgccggg ttctttattg gtctggcgta atgctcgatt
     8941 tgtagaccgg ataaaacgta ttaagcgttg catccggcaa tcgcgctatt gcaaataatt
     9001 tttactgcat catcccggag aatttattct ccgggtccat ctccagcgcc agtcctgaca
     9061 actgctttct gtttttaatt agatatcctt tcttttaatt aataaaccgt cgtgaatgaa
     9121 ttgcgcgaga acatataaaa gatgtcgata agaaacgcct aaatactctg ccgcttgcgt
     9181 atgcttttcg tgataaagat caccttcttg tgagagtaat ataaatgctg ccaggcgatt
     9241 cactaacgga aatgattgat tctgagttaa agaaacaata ttacgataat ttttatgact
     9301 taaggtgacg caaagttttc gtaaaaatag ggtgtcgttt aataacagcg gacggtaatg
     9361 tttcataggg agcgcaaggc accaacactc ttcaatagcc tgcaccgcac gcggttcatg
     9421 gtctttgtcg attaactcaa tctcgccaat aaaacaggga gcggcaaaga aatcgatcag
     9481 cgacacgcga ccattggcaa gcgtggcgta aagcctggcg cggcctcgcg tcagataaaa
     9541 cagccatgac ggctgttgcc cttcctgcac aatgtagtca cgcgctaaaa aatgaaacag
     9601 ccgcgtatcg gctgaaacat ccgttgaaaa gcaatcctta aacgcagatt cactcatcaa
     9661 ttgctgctta aggtcattat tgtggatttc tttcacgatg tttatccgct atgagatttc
     9721 tcatatttag catacgtacg ctgttgttaa gattcaatca ttggcccggt cgaattttct
     9781 ttgctgtaga aaaaaataac gcaactggaa acagaggaaa taaacaatgg aaaagagaaa
     9841 aattattctg gattgtgagc cgggtcatga tgatgctatt gcaatgatga tggcagcgaa
     9901 acatccggca atagatttat taggcatcac tattgtagcg ggtaatcaga cgcttgataa
     9961 aacattaatt aatggcctga atgtttgcca gaaactggag attaatgttc cggtttatgc
    10021 ggggatgccg caacccatta tgcgtaaaca aatcgttgcc gataatattc acggtgaaac
    10081 cggactggat ggcccggtat tcgagccgct gacccgccag gcagaaagta cccatgcggt
    10141 gaaatatatc atcgataccc tgatggcaag cgatggcgat atcactctgg tgccggttgg
    10201 tccgctttca aatatcgcgg tggcaatgcg tatgcaaccc gcaatcctgc ccaaaatccg
    10261 tgaaattgtg ctaatgggcg gcgcttacgg tacaggcaac ttcaccccat ctgccgagtt
    10321 caatatcttt gccgacccgg aagccgcccg cgtggtgttc acctccggcg ttccattagt
    10381 gatgatgggc ctcgatctta ccaaccagac cgtttgcacc ccggatgtaa ttgctcggat
    10441 ggaaagggta ggcggccctg ccggagagct gttcagcgac atcatgaact tcactctcaa
    10501 aacgcagttc gaatactacg gcctggctgg cggcccggtg catgacgcca cctgcattgg
    10561 ttatctgatc aaccctgatg gcattaaaac ccaggatatg tacgtcgaag tggacgtcaa
    10621 cagcggccct tgctacgggc gtacggtctg cgacgaactg ggcgttcttg gcaagcccgc
    10681 taataccaaa gtcggcatca ctattgatac cgactggttc tggggcttag tcgaagagtg
    10741 cgtgcgcggc tacatcaaaa cccattaagt gttgataaca agccgggaca gtatcgcaag
    10801 atgcaggccc ggctatttct gatgggcaga aagccgtcgc ccaaaatgac atgaagagat
    10861 aatcactatg gatgtcatga gaagtgttct gggaatggtg gtactgctgg caattgcgtt
    10921 tttactgtca gtaaacaaga agaagatcag cctgcgtacc gttggcgcgg cgttagtgtt
    10981 acaggtcgtg attggcggga ttatgctttg gttaccgcca gggcgttggg tcgcagaaaa
    11041 agtcgctttt ggcgtccaca aagtgatggc gtacagcgac gcaggtagcg catttatctt
    11101 cggttctctg gtcggaccga aaatggatac cttatttgat ggcgcaggat ttatctttgg
    11161 tttcagggta ttaccggcaa ttatcttcgt cactgcactg gtgagtattc tctactacat
    11221 cggtgtgatg gggatcttaa ttcgcattct cggcggtatc ttccagaaag ctttaaatat
    11281 cagcaagatc gagtcattcg tcgcggtcac caccattttc ctcgggcaaa acgaaattcc
    11341 ggcgatcgtc aaacccttta tcgatcgtct gaatcgcaat gaattattta cagcgatttg
    11401 tagtggcatg gcctcgattg ctggttcgac aatgattggt tacgccgcac tgggcgtacc
    11461 tgtggaatat ttgctggcgg catcgttaat ggcgatccct ggcgggatct tgtttgcccg
    11521 cctgttaagc ccggctacgg aatcttcgca ggtttctttt aataacctct ctttcaccga
    11581 aacaccgcca aaaagcatta ttgaagccga tgcgacaggg gcaatgaccg ggctgaaaat
    11641 cgccgccggt gtagcgacag ttgttatggc atttgtcgcc atcattgcgt taattaacgg
    11701 tattatcggc ggcgttggcg gctggtttgg ttttgaacat gcctcgctgg agtccatttt
    11761 aggttacctg ctggccccac ttgcgtgggt aatgggtgtt gactggagtg atgcaaatct
    11821 tgccgggagt ttgattggac agaaactggc aataaatgaa tttgtcgctt atctcaattt
    11881 ctcaccctat ctgcaaacgg ctggcactct ggatgctaaa accgtggcga ttatttcctt
    11941 cgcgttgtgc ggtttcgcta actttggttc tatcggggtg gtggtggggg cgttttctgc
    12001 ggttgcgcca caccgtgcgc cggaaatcgc ccagcttggt ttacgcgcgc tggcggcggc
    12061 gacactttct aatctgatga gtgccaccat tgccgggttc tttattggtt tagcgtagtg
    12121 tgcaatttat gagccggatt agatgcggtc aagcgtcgaa tccggcaaca ttgttacacc
    12181 attggcacta atgacagcgc gttatcggca gacagggtag ggttgtttaa gctgccgctg
    12241 gcgcgtgaaa ttgccgcgca ggccatcgca aaacgggcgc tgtcacggaa attgtttccc
    12301 tccagaaagc tatataccag acccgccata aaaccatcgt cagcaccaaa actgtctacc
    12361 gtcgtatgcg ctggcacagt cagcaaaaat tgttcaccat ccttttcgct gcaataaact
    12421 gactcatcgg gcaaataaac aaacagttgc tgaacacctt gctgatgcaa tgcattcact
    12481 gcggcattac ggtcagcatc gctggtgatc gcctgtcccc ataaaatttc cagctccggt
    12541 aaagtgggtt tcagggtgtg aatatgcgcc agccagtgtt tgattttgcc cgctttgaat
    12601 tctgaaacgg tatcgacaaa caccgggatc tcatcagcga gggtaaagac ccattccagc
    12661 gcctcggctg tcaggttgca atcagccagt accacgcccg catgacgaag taaatcgcgc
    12721 gacccgttca gtagttgcgg tgtcaactgt tccagcagat gggtatcgtt aattgccagc
    12781 acggtttgat cgtctcgatt ggcaattgcc agatacgtcg atgtgctttg accatgcaaa
    12841 cgaacgcagc cggagacatt cacgccggca cggcgcgttt cttccaggag catttcgcca
    12901 taaaagtcat cgccaatcac tgaaagcaaa tggacgtcac ggcctaacag cgccagattg
    12961 tgggcgatat tgcgtccaac gccgcctgcc gaacaatgaa ttgtaccggg atgagaagcc
    13021 gcttgcgggt aatggatatc cgccatcccg cgaatatcca tattgattgt ccccaccact
    13081 acgcagtatt cctgctcggt gagaatgtaa cctttgtctt taatccggcc tttgcgcatt
    13141 aaatccataa tatgcgccgc aacgcgcgaa cgactgattt gcagcatgtc cgcaatttcg
    13201 ttctgctgaa tcagcgggtt acgccgtaaa attgcaagga tctccttttc ccggttattt
    13261 atcttcaggc taccgctttt tcagtttgtt gcgctttcag ccaggcgatc tcttctgccc
    13321 agatatccgg gttgatggtt tcaaggatca gcggaatgcc gtcgaaacgg tcgttctgca
    13381 taatccagcg gaacgcatca tgaccgatat tgccttcacc gaggctatga tggcggtcaa
    13441 cgcggctgcc aaaggtactt ttcgcatcgt taaggtgcat cccgcgcaaa tacttaaagc
    13501 cgacaatacg ggcaaaatca gcgaacgttt tttcgcattc ggctggagta cgcaaatcat
    13561 acccggcagc gaaagcatgg caggtatcaa tgcagacgcc gacgcgggat ttatcttcca
    13621 cgccgtcgat aatcgccgcg agatgttcga atttaaaccc taagttactg ccctgaccgg
    13681 cggtgttttc aatcaccgct gttacacctt gagttttatc cagcgcaatg ttgatggatt
    13741 cggcaatacg cgcaaggcaa tcctcttctg aaatctgcat cagatggctg ccagggtgga
    13801 agttgagcaa agaaagcccc agttgttcgc aacgctgcat ttcatctata aaggcatcgc
    13861 gcgatttttc cagagcttca gtgaccggat gtccgaggtt gatcagataa ctgtcgtggg
    13921 gaagaatttg cgccgatgtg tagtgatatt tttcacaggc ggctttgaat tcatcgatgg
    13981 tttgcgtcgt gagcggtgcg gcacgccact gacgttggtt tttggtgaac aaggcaaacg
    14041 cggttgcgtc gatttcggcg gcgcgaattg cggcatttgc cagaccgcca gcagcactaa
    14101 cgtgcgctcc aatgtatttc atgcgaggac tcctgttaaa cccgctggag gaaaacggta
    14161 atgatagcgg gttaacagga gcaagaagta gtggtttatg cgatgacgct ttgaatcaca
    14221 tagttaatcg caccaccacc aacaatcagc caggcaaaca gtaccagtgc catcagcaga
    14281 ggtttcgccc cagctttttt cagcgcgctg acgtgagtgg tcagacccag cgccgccatc
    14341 gccattgcca gcaggaaggt atccagcgtt accagcatgt tcaccacgct ctgcggtaac
    14401 aggtggaacg agttaaagat ggcaactacg atgaacaaga tggcaaacca cggaatagtg
    14461 attttgcttt tctcgccgct gttcgcccca gacagctgtt taacacgcgc cgccagcagg
    14521 atgaggaacg gagccagcat catcacgcgc agcattttgg aaataactgc tgcgttttct
    14581 gcatccgggc tgatggcatg acctgccgcc accacctgcg ccacttcgtg cacagtagaa
    14641 ccaatgtaga taccgaaagt ttccggacta aaccattgag acatcagcgg atatatcgcc
    14701 gggtagagga aaatcgcgac ggtcccgaag ataacaacgg ttgcaacagc cacggttact
    14761 ttactggctt ccgctttcac taccggctca gtcgccagta ccgcggcagc accacagata
    14821 ctgctaccgg caccgatcaa ccaactggtg tgcttatcca gaccaaacac tttctgcccc
    14881 aggaagcaag ccagcaggaa ggtactggac agcgtcagca cgtcaatgat gatcccactg
    14941 ataccgacat cggcaatttg cgagaacgtc agacggaagc cataaagaat gatacccaga
    15001 cgtaataaat attgcttggc aaacagcaca ccaccgtcac agcttttcca gatgtgcgga
    15061 tagatggtgt tgcctaaaac catccccaac aagattgcga gggtgagggc actaaacccg
    15121 gcacccgcaa ccgcgggaat ggaaccaccc cacagggcga ccccggtgat aactgcactc
    15181 agggctaacc ccggaataaa atgccacagt gtacgatgtt gtttctgtaa ggtgatattc
    15241 gtcataaccc tctcctttaa ccgggcataa ggttacgact aactggttta aaaataaaat
    15301 tgattatata tttataatta atctttataa gtggtaagcg actatgcaca tcaccctccg
    15361 gcagttggaa gtttttgcag aagtattgaa aagtggatca accacccagg cgtcggtgat
    15421 gctggcgttg tcgcaatcag cagtgagcgc agccttgact gacctggaag ggcagcttgg
    15481 cgttcaactg tttgatcgcg tggggaaaag actggttgtt aatgaacacg ggcggctgct
    15541 ctatccgcga gcgttggcac tgcttgaaca ggcggttgaa atcgaacaac tgtttcgcga
    15601 agacaacggc gcgattcgta tctatgccag tagtaccatc ggtaactaca ttctgcctgc
    15661 agttatcgcc cgttatcgcc atgattatcc gcagttgccg attgaactta gcgttgggaa
    15721 cagccaggac gtgatgcaag cggtgctgga tttccgcgtt gatattggct ttattgaagg
    15781 accgtgccac agcactgaaa tcatttctga accgtggctg gaagacgagc tggtggtttt
    15841 cgccgcgccg acttcgccgt tggctcgtgg tccggtcacc ttagaacagc tggccgctgc
    15901 gccgtggatc ctgcgtgaac gcggttccgg cacgcgggat attgtcgatt atctgttgct
    15961 gtcacattta ccgaagtttg agatggcgat ggaattaggt aactccgagg caatcaaaca
    16021 tgcggtgcgt catgggttgg gaattagttg cctgtcgcga cgtgtgattg aagatcaatt
    16081 gcaggcaggc acattaagtg aagttgcggt ccctctgccg cgcctgatgc gtacgttgtg
    16141 gcgtatacat catcggcaaa aacacctttc caacgcgcta cggcgctttc tggactattg
    16201 cgatcccgca aatgtgccgc gttaagttgc tgtacaagaa catgctggtg ctgtgtcgat
    16261 ttcgtgacgc agcgccttca gcatgcattc gccagaaaag agattggctg ctttacttat
    16321 aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat ttctgccgga aggattgcca
    16381 atcgtctgct acaatcgcgc catcattttt aagatggata gcatttttgt atgggttccg
    16441 aaactaaaac tacagaagcg ccgggcttac gccgtgaatt aaaggcgcgt cacctgacga
    16501 tgattgccat tggcggttcc atcggtacag gtctttttgt tgcctctggc gcaacgattt
    16561 ctcaggcagg tccgggcggg gcattgctct cgtatatgct gattggcctg atggtttact
    16621 tcctgatgac cagtctcggt gaattggctg catatatgcc ggtttccggt tcgtttgcca
    16681 cttacggtca gaactatgtt gaagaaggct ttggcttcgc gctgggctgg aactactggt
    16741 acaactgggc ggtgactatt gccgttgacc tggttgcagc acagctggtc atgagctggt
    16801 ggttcccgga tacaccgggc tggatctgga gtgcgttgtt cctcggcgtt atcttcctgt
    16861 tgaactacat ctctgttcgc ggctttggtg aagcggaata ctggttctca ctgatcaaag
    16921 tcacgacagt tattgtcttt atcatcgttg gtgtgctgat gattatcggt atcttcaaag
    16981 gcgcgcagcc tgcgggctgg agcaactgga caatcggcga agcgccgttt gctggtggtt
    17041 ttgcggcgat gatcggcgta gctatgattg tcggcttctc tttccaggga accgagctga
    17101 tcggtattgc tgcaggcgag tccgaagatc cggcgaaaaa cattccacgc gcggtacgtc
    17161 aggtattctg gcgaatcctg ttgttctatg tgttcgcgat cctgattatc agcctgatta
    17221 ttccgtatac cgatccgagc ctgctgcgta acgatgttaa agacatcagc gtcagtccgt
    17281 tcaccctggt gttccagcac gcgggtctgc tctctgcggc ggcggtgatg aacgcagtta
    17341 ttctgacggc ggtgctgtca gcaggtaact ccggtatgta tgcgtctact cgtatgctgt
    17401 acaccctggc gtgtgacggt aaagcgccgc gcattttcgc taaattgtcg cgtggtggcg
    17461 tgccgcgtaa tgcgctgtat gcgacgacgg tgattgccgg tttgtgcttc ctgacctcca
    17521 tgtttggcaa ccagacggta tacctgtggc tgctgaacac ctccgggatg acgggtttta
    17581 tcgcctggct ggggattgcc attagccact atcgcttccg tcgcggttac gtattgcagg
    17641 gacacgacat taacgatctg ccgtaccgtt caggtttctt cccactgggg ccgatcttcg
    17701 cattcattct gtgtctgatt atcactttgg gccagaacta cgaagcgttc ctgaaagata
    17761 ccattgactg gggcggcgta gcggcaacgt atattggtat cccgctgttc ctgattattt
    17821 ggttcggcta caagctgatt aaaggaactc acttcgtacg ctacagcgaa atgaagttcc
    17881 cgcagaacga taagaaataa gtttcctccc ttccttgcta agccctctca accgagaggg
    17941 ctttttcaat tccatttccc tgacaaatca tgcggatata aaatttaaca tttggattga
    18001 taattgttat cgtttgcatt atcgttacga cgcaatcaaa aaaggctgac aaatcagagg
    18061 ctgttccggc tttctgggat gatcacctgc atacaaataa gtccaccgcg atgctgccgt
    18121 acgcaagggg acgtaaagaa gatgtgagcg atacccattt tattttcgta gttacctcat
    18181 ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt cgggctgtgt ttgtccgcta
    18241 tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg cgaaacgatg gttgtcactg
    18301 catcttccgt ggaacaaaat cttaaagatg cacccgccag tatcagcgtc attacccagg
    18361 aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt cctcaaagaa gtgcctggcg
    18421 tacaactgac gaacgaaggg gataaccgta agggcgttag tattcgtggt ctggacagca
    18481 gctacaccct gattcttgtc gacggtaaac gcgttaactc ccgcaatgcc gtcttccgcc
    18541 acaatgattt cgatctgaac tggatcccgg tcgattccat cgaacgtatt gaagtggttc
    18601 gtggcccgat gtcgtcgcta tacggctccg atgcgctcgg cggtgtagtg aatatcatca
    18661 ccaaaaaaat cggtcagaaa tggtcgggta ccgttaccgt cgataccacc attcaggaac
    18721 atcgcgatcg cggtgatacc tataacggtc agttctttac cagtggacca ttaattgatg
    18781 gtgtgctggg aatgaaagct tacggcagcc tggcaaaacg tgaaaaggat gacccgcaaa
    18841 actcaacgac caccgatacc ggagaaacgc cgcgtattga aggattctcc agccgcgacg
    18901 gcaatgtcga atttgcctag acaccgaatc aaaatcacga ttttactgcc ggatacggtt
    18961 tcgaccgtca ggatcgtgat tccgattcgc tggacaaaaa ccgcctggaa cgccagaact
    19021 actgcgtcag ccataatggg cgttgggatt acggcaccag cgaactgaaa tcctacggtg
    19081 agaaagtcga gaacaaaaac cctggcaaca gcagcccgat aacttccgaa agcaatacgg
    19141 tcgacggcaa atacacgttg ccgctgacgg cgattaatca gtttctcacg gttggcggtg
    19201 aatggcgtca cgacaaactt agcgatgcgg tgaacctgac cgggggaacc agctccaaaa
    19261 cgtctgccag ccagtacgcg ctgtttgtgg aagatgaatg gcggatcttc gagccgctgg
    19321 cgctgacgac cggcgtgcgt atggacgatc acgaaaccta cggtgaacac tggagtccgc
    19381 gtgcctacat ggtttataac gccaccgaca ccgtaacggt gaaagggggc tgggcgacgg
    19441 catttaaagc accttctctg ttgcaactta gccctgactg gacgagcaat tcctgccgtg
    19501 gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga aaccagcgaa agttgggagc
    19561 tggggcttta ctacatgggt gaagaaggct ggctggaagg gggtgaatcc agcgttatcg
    19621 ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg tacgtctgac gtcaacgctg
    19681 caccgggcta ccaaaacttt gttggttttg agacgggcgc taacggacgg cgcataccgg
    19741 tatttagcta ctacagcgtt aacaaagctc gtattcaggg cgtggaaacc gaactgaaaa
    19801 ttccgttcaa cgatgaatgg aaactgtcga tcaactacac ctacaacgat ggtcgtgatg
    19861 tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt ccatactgct aacggtacgc
    19921 tggactggaa accgctggcg ctggaagact ggtcattcta tgtttctggt cactataccg
    19981 ggcagaaacg cgccgacagc gcgacggcta aaacaccggg cggttatacc atctggaata
    20041 ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg cgcaggcgtg ctgaaccttg
    20101 gcgacaagga tctcagtcgt gacgactaca gctataacga agacggacgt cgttacttta
    20161 tggcagtgga ttatcgcttc tgatgagaag atgcccggcg aaccgggcgg actttcactt
    20221 cagtaaatac tgcgcatgga agcgcaggtg atcctctata aaagaggcga tgaagtagta
    20281 actgtgatca tatcccggct gaatacgcag cgtcatcggc cacgctttct ggcgcgcggc
    20341 ttctgccaat accgcaggtt gcaactggtc ggcaagaaac tgatcattat cgccctgatc
    20401 gataagcgtc gggatcgcat cctgcgcgtt actggcatac atcagcgcgc aactatccca
    20461 ttccagcatg catttttgtc ctcacctaaa taggtgctaa acgctttgat tccccacggg
    20521 acgctgcacg gattcacaat gggcgcaaag gccgaaacgc tggtgtattt acccggattt
    20581 ttcagcgcca taatcagcgc accgtgacca cccattgagt gaccgctaat ggcgcagcgg
    20641 tcgctgacat taaattgcga ctgaaccagc gccggtaatt catcgcgcag ataatcatac
    20701 atccggtaat gcgtcgccca cggcggttgc gtggcattaa gataaaagcc tgcgccctgg
    20761 cctaaatcgt agccatcgtc gttggcaacc ttttcgccgc gcgggctggt gtctggcatc
    20821 accagtacaa tccccagttc cgccgctacc cgctgggcac ccgccttggt ggtgaagttc
    20881 tcgtcattgc aggttaatcc ggaaagccag tacagcactg gtggcggggt gtgatcacga
    20941 ggggggggga gaaagatact gaacgtcatc gggcagttta aggtactgga gtcgtgtcgc
    21001 cagcgttgct gccagccttc aaaacagcgg tgctcttcga gcatttccat gcgtggctcc
    21061 ttgttgtgtt gtttgcaatt tcttcatcat accgtttatt caatgtgctg tgagtaactt
    21121 tcacttccgt atttgcataa cgatgtttta acatctgctg atgaaaggca gcggcaatta
    21181 caataattat cgctgtgaat actggattat gtgcgccgcc tcacgcacaa taatcaagct
    21241 gtaaatcagc ttaataactt tgcccccacg cagggtggag gcgtcacacc tgcaggagaa
    21301 atcataaatg ccatcactca gtaaagaagc ggccctggtt catgaagcgt tagttgcgag
    21361 aggactggaa acaccgctgc gcccgcccgt gcatgaaatg gataacgaaa cgcgcaaaag
    21421 ccttattgct ggtcatatga ccgaaatcat gcagctgctg aatctcgacc tggctgatga
    21481 cagtttgatg gaaacgccgc atcgcatcgc taaaatgtat gtcgatgaaa ttttctccgg
    21541 tctggattac gccaatttcc cgaaaatcac cctcattgaa aacaaaatga aggtcgatga
    21601 aatggtcacc gtgcgcgata tcactctgac cagtacctgt gaacaccatt ttgttaccat
    21661 cgatggcaaa gcgacggtgg cttatatccc gaaagattcg gtgatcggtc tgtcaaaaat
    21721 taaccgtatc gtgcagttct ttgcccagcg tccgcaggtg caggaacgtc tgacgcagca
    21781 aattcttatt gcgctacaaa cgctgctggg caccaataac gtggctgtct cgatcgacgc
    21841 ggtgcattac tgcgtgaagg cgcgtggcat ccgcgatgca accagtgcca cgacaacgac
    21901 ctctcttggt ggattgttca aatccagtca gaatacgcgc cacgagtttc tgcgcgctgt
    21961 gcgccatcac aactgattaa aaggcaggaa ccatggagcg caacgtcacg ctcgattttg
    22021 ttcgcggcgt cgccattctg gggatcctgc tattaaacat cagcgccttt gggctaccaa
    22081 aggcggctta tctcaatccc gcctggtacg atgctattac gccgcaggat gcatggacct
    22141 gggcatttct cgatctcatc ggccaggtga aattcctcac gctttttgcg ctgctgtttg
    22201 gtgctggcct gcaaatgttg ctgccccgtg gcagacgctg gatccagtcg cggttaacgc
    22261 tgttagtctt gttgggcttt attcacggtt tattgttctg ggacggcgat attctgctgg
    22321 cttacgggct ggtggggctt ggcgttttgc tgttattggg gctgatttcc gacagccaga
    22381 ccagccgcgc ctggacgccg gatgcatcgg ctattttgta tgaaaaatac tggaagcttc
    22441 acggcggcgt tgaagcgatc agtaatcgtg ccgatggtgt tggcaacagt ttactggcac
    22501 tgggcgcaca gtatggctgg caactggcag ggatgatgct cattggtgcg gcgctgatgc
    22561 gcagcggctg gctgaaaggg cagttcagct tacgtcacta tcgtcgtact ggttttgtgc
    22621 tggtggcgat tggggtgacc attaaccttc ctgccatcgc cctgcaatgg cagctggact
    22681 gggcgtatcg ctggtgtgcc ttcttacttc aaatgccgcg ggaactgagt gcgccgtttc
    22741 aggcgattgg ctatgcgtcg ctgttttatg gcttctggcc gcaattgagc cgctttaagc
    22801 tggtgcttgc gatcgcctgc gtcggacgga tggcgctgac caactatcta ttgcaaacgc
    22861 tgatttgtac cacgcttttt taccacctcg gtttgtttat gcattttgac cgcctggagc
    22921 tgctggcgtt tgttattccg gtatggctgg cgaatatcct cttctctgtt atctggctgc
    22981 gttacttccg ccaggggccg gtggaatggc tctggcgtca gttaactttg cgtgctgccg
    23041 gaccggcaat atctaaaaca tcaagataac gatctggatt acattcgtta acaaaacggc
    23101 tgtaaccgtt tccattgctg tgactcgatt cacgaagtcc tgtattcagt gctgacaaaa
    23161 tagccgccag caagcagtca tttactgcaa tctcataaca ggtagtgaat atgatcacca
    23221 ttcgtgatgt agcgcgtcag gctggcgtct ctgtggcaac ggtttcccgg gtgctcaata
    23281 acagcacgct ggtcagtgcc gacacgcgtg aagcagtaat gaaagccgtg agtgagctgg
    23341 attatcggcc aaacgccaat gcccaggcgc tggcaactca ggttagcgac accattggcg
    23401 tggtggtgat ggacgtttct gatgcgtttt tcggcgcgct ggtaaaagcg gtggatctgg
    23461 tcgcccagca gcatcagaaa tacgtgctaa tcggcaatag ctatcatgaa gcggaaaaag
    23521 agcgtcacgc cattgaggtg ttaattcgcc agcgttgtaa tgcgttgatt gttcactcaa
    23581 aagcattgag tgacgatgaa ctggcgcaat ttatggataa cattcccggt atggtgttaa
    23641 tcaaccgcgt tgtgccgggg tacgcccatc gttgcgtttg cctggataat ctcagcggtg
    23701 cccgaatggc gacgcgcatg ttgctgaata acggtcatca acgtattggt tatctttctt
    23761 ccagccacgg cattgaagat gacgccatgc gtaaagcagg ctggatgagt gcgttgaaag
    23821 agcaggatat tattccgccg gaaagctgga ttggcactgg tacgccggac atgccgggcg
    23881 gtgaggcggc gatggttgaa ctgctggggc gcaatctaca acttaccgct gtatttgctt
    23941 ataacgacaa tatggccgct ggcgcactga cagcattaaa agataatggc attgcgattc
    24001 cgttacatct ctcaatcatc ggtttcgatg atattcccat cgcccgttac accgacccgc
    24061 aattaacgac cgtgcgttat cccattgctt caatggctaa attagccacc gaactggcct
    24121 tgcagggggc agcaggcaat attgatcctc gtgccagcca ctgttttatg ccgacgttag
    24181 tgcgtcgcca ttctgtcgca acgcgccaga atgcggcggc gatcactaac tcaacaaatc
    24241 aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc
    24301 tatgattgca ccgttttaat gttgtaaccc gtatgtaaca gtgaataatc actttcaccg
    24361 aggtaatagc gtcataacaa caattaaagc cgttttctgg agcgttaccg ggcatggaag
    24421 aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc tactgccccg
    24481 ccgagcattt atctcaagca ctaccctgca taagaaaaac cggagatacc atgaataaga
    24541 aggtgttaac cctgtctgct gtgatggcca gcatgttatt cggtgccgct gcacacgctg
    24601 ctgatactcg cattggtgta acaatctata agtacgacga taactttatg tctgtagtgc
    24661 gcaaggctat tgagcaagat gcgaaagccg cgccagatgt tcagctgctg atgaatgatt
    24721 ctcagaatga ccagtccaag cagaacgatc agatcgacgt attgctggcg aaaggggtga
    24781 aggcactggc aatcaacctg gttgacccgg cagctgcggg tacggtgatt gagaaagcgc
    24841 gtgggcaaaa cgtgccggtg gttttcttca acaaagaacc gtctcgtaag gcgctggata
    24901 gctacgacaa agcctactac gttggcactg actccaaaga gtccggcatt attcagggcg
    24961 atttgattgc taaacactgg gcggcgaatc agggttggga tctgaacaaa gacggtcaga
    25021 ttcagttcgt actgctgaaa ggtgaaccgg gccatccgga tgcagaagca cgtaccactt
    25081 acgtgattaa agaattgaac gataaaggca tcaaaactga acagttacag ttagataccg
    25141 caatgtggga caccgctcag gcgaaagata agatggacgc ctggctgtct ggcccgaacg
    25201 ccaacaaaat cgaagtggtt atcgccaaca acgatgcgat ggcaatgggc gcggtagaag
    25261 cactgaaagc acacaacaag tccagcattc cggtgtttgg cgtcgatgcg ctgccagaag
    25321 cgctggcgct ggtgaaatcc ggtgcactgg cgggcacagt actgaacgat gctaacaacc
    25381 aggcgaaagc gacctttgat ctggcgaaaa acctggccga tggcaaaggt gcggctgatg
    25441 gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc gtagataaag
    25501 acaacttggc tgaattcagc aagaaataag actgattggg cgcagtcgtt tactgcgccc
    25561 ttattgtatg gccaacaagg taaaattata tggtcagctc aacgactccg tcttccgggg
    25621 aatacttgtt ggaaatgagc ggtatcaaca agtcctttcc tggtgttaag gcacttgata
    25681 acgttaattt aaaagtccgg ccacattcta tccatgcatt aatgggggaa aacggtgcag
    25741 gaaaatcgac attattaaaa tgcctgtttg gtatttatca aaaagactcc ggcaccattt
    25801 tattccaggg taaagagatc gatttccatt ccgccaaaga ggcactggaa aatggtattt
    25861 cgatggtaca ccaggagtta aacctggtat tacaacgttc ggtgatggat aacatgtggc
    25921 tggggcgata tcccaccaaa ggcatgtttg tcgatcagga caaaatgtac cgcgaaacca
    25981 aagcgatttt tgatgaactg gatattgata tcgatccgcg tgcgcgcgtc ggcacattat
    26041 ccgtttcgca aatgcagatg atcgaaatcg ccaaagcgtt ttcctataac gcgaaaattg
    26101 tgattatgga tgaaccgact tcttcgttaa ccgaaaaaga ggtcaatcat ctgttcacta
    26161 ttattcgtaa attaaaagag cgcggctgcg gtattgttta tatctcgcat aaaatggaag
    26221 aaatcttcca gttatgtgat gaagttaccg tattgcgcga cggtcagtgg atcgccaccg
    26281 aaccgctggc aggactgacg atggacaaga tcatcgccat gatggttggg cgttctctta
    26341 atcagcgttt tcctgacaaa gaaaacaagc cgggcgaagt catcctcgag gtgcgtaacc
    26401 taacgtcact gcgccagccg tcgattcgcg atgtctcgtt tgatctgcat aaaggggaga
    26461 tcctcggtat tgccgggctg gtgggggcga aacgtaccga tattgttgag acgttatttg
    26521 gtattcgcga gaaatcggct ggcaccatta cgttgcacgg caaacagatc aataaccata
    26581 atgccaacga agccataaac cacggatttg cactggtaac tgaggagcgc cgctcaacgg
    26641 gaatttatgc ctatctggat attggtttta actcgttaat ttccaatatt cgcaactaca
    26701 aaaataaagt tggtttactg gataactcgc ggatgaaaag cgatacccag tgggtgattg
    26761 attcgatgcg ggtaaaaacg ccgggtcatc ggacgcaaat tggttcgctc tccggtggta
    26821 atcagcaaaa ggtgattatt ggtcgctggc tactaacgca accagaaata ttaatgctcg
    26881 atgaaccgac gcgcggtatt gatgtcgggg cgaagtttga aatttatcag ttaattgccg
    26941 aactggcgaa gaaaggcaag gggattatta ttatctcctc tgaaatgcct gagttgttag
    27001 ggataacaga ccgtattctg gtcatgagca atggtctcgt ttccggaatt gtcgatacaa
    27061 aaacaacaac gcaaaacgaa attctgcgtc ttgcgtcttt gcacctttaa gattaggggc
    27121 ttcccatgag tgcgttaaat aagaaaagtt ttcttactta cctgaaagag ggcggtattt
    27181 acgtcgttct tttagttttg ctggcgatta ttattttcca ggacccaaca tttttaagtc
    27241 tgttgaactt aagtaatatt ctcacccagt catcggtgcg tattattatc gcgctcggtg
    27301 tggcagggtt aattgtcacc caggggaccg atctttctgc tggtcgtcag gtagggctgg
    27361 cggcagtggt ggctgcgaca ttattgcagt ccatggataa cgccaacaaa gtgttcccgg
    27421 aaatggcgac gatgccgatt gcgctggtta ttctgattgt ctgtgccatt ggtgcggtga
    27481 tcggtttgat caacggtctg attatcgctt atctcaacgt gacgccgttc attaccacgc
    27541 tcggcacgat gatcatcgtc tatggcatca actcgctcta ttacgacttt gtcggggcgt
    27601 cgccaatttc tggttttgac agtggcttct ctacctttgc tcagggcttt gtcgcgctgg
    27661 ggagtttccg tctctcttac atcaccttct acgcgttgat tgcggtggcg ttcgtctggg
    27721 tgttgtggaa caaaacccgc ttcggtaaga acatttttgc cattggcggt aacccggaag
    27781 cggcaaaaat atctggtgtc aacgtcggcc tgaacctgct gatgatctac gcgttgtctg
    27841 gcgtgttcta tgcctttggc gggatgttag aagccggacg tatcggctct gccaccaaca
    27901 acctcggctt tatgtatgag ctggatgcta tcgcggcgtg cgtggtaggc ggtgtatcgt
    27961 tcagcggcgg tgtggggacg gtgattggcg tggtgaccgg ggtaattatt tttaccgtca
    28021 tcaactatgg cctgacgtat atcggcgtaa acccatactg gcagtacatc atcaaagggg
    28081 cgattattat cttcgccgta gcgctggatt cactgaaata cgcgcgtaag aagtgatgtg
    28141 attgcggata ttgccggatg cgtctctggt tttacaacgt taccgggtgt tctttctcgc
    28201 ctttcttaaa cttcacttcc ccgagctcaa tacaacccac cgggcagacg tgaccacaaa
    28261 gcagacaacc cacacatttc tcggtattac aatgcggtgt gcgggttttc tcgctccatt
    28321 ccatcgcctg gtgaccgccg tcgtaacagg aaatataaca gcgtccacag ccaacacatt
    28381 tgtcaagatt gatacgggga tagacaatat aactgcggtc taaatcttcc gccgggacaa
    28441 tattgttatt cgccagacct accatctcct gcaacgaatc aaatccctga tcggcgagat
    28501 aatgcgataa cccgctcgcc atatcttcta cgatgcgata gccgtactgc ataatgccgg
    28561 tggtgacctg taacgttgct gcgccgagca ataaaaactc cgccgcatct tcccaggttt
    28621 caatgccgcc gataccgctg attgggaaat cgcgcagttc tggatgggtg cgcatttgct
    28681 gaatgaagcg cagggcgatc ggttttaccg ctttaccgga atatccggaa atactcgatt
    28741 taccgttaac gatcggcata ccgattttct gattaagatc gatattggtg atggatttaa
    28801 cggtgttaat tgccgcaatg ccatctgcgc cgccgcgctt cgccgccagc gccacttcgc
    28861 acatatcacc gatattcggc gtcatcttcg ccagcattgg cagcgtggaa ccccgtttca
    28921 ctgcccgaca atatttttct accagctccg ggctttgccc gacatcgcta cccatcgcat
    28981 gagaagtcat ttgcggacag gagaagttac actcgatcat atccgcgcca gctgctccca
    29041 ttgctgctca ttttccccca tgatcgaagc gatcaatact ttgtccgggt aatcttcctt
    29101 cagccgacgc agggcggtca gattctcttc caacggatgt tcagcaatct gctccatatt
    29161 tttgaagccg ataaaaccgg tatcttcttt caccagatga tcaaaacgcg gcgagacttc
    29221 gttggcgata aaaaagccga tcgttttaaa caccacaccg ccccaacctg tgtcgtaggc
    29281 tttggcacac atctcatagc agttgcctac cggcgaagaa gagaggcaga acgggttggg
    29341 aaagttcacg ccgcaaaaag taatcgaaag atctttcgtt aacatgagca agctccctct
    29401 aaatagtgat gaatcgcctc ggcggcttct ttcccggttt tcacggcata gaccacggtt
    29461 ttgtcaccct caacaatatc gccagcagca aagacttgcg gatcgcggat ctgataattt
    29521 tgtgttttga tggtgttacg ctgctgctct aactccgcaa aggcatccag tctggcatgc
    29581 tgaccgacgg cgagaataat tttatctgcc gccatcgtca gttcgcctga taaccgtaca
    29641 tgcttaaacg tgactttatt gccttccacg gctactggcg tgaatccatc gatgatcgaa
    29701 acgcccagct cccgggcgct ggcaaactct ttttcgcttg ccggaaactc atctaactct
    29761 tcacgtgcta cgcaagttac cgcctgacag ccgagaactt tcagcgtgct ggctacgtcc
    29821 atcgcgacat caccgccgcc gataattaat gcgctttgtg gaatgctgat atcgccttgc
    29881 gcctgtcgta cacgttgcag gaagtcgacg gcaatctcaa cgtcgctatg ctcaaacagc
    29941 ggtagcccgg aaccgcttga taaccccacg gtgaccagta ccgcgcggtt ttccgctttc
    30001 agttgctcca gggtgagtgt tttaccgatt tcgttattgc acttaatggt cacgcccatt
    30061 ttttcaatac gggcgatctc tgcatccagc actgattgcg gtaaccggaa ttgcggaata
    30121 ccgttacgca accagccacc ggggtgcgct tctttctcat aaatcgtgac gtcataaccc
    30181 tggtttgtca gtgtcacact ggcctgcaat ccggcaggac ctgcgccaat aatcgcgact
    30241 ttgccgagcg ttttagtacc gggctgataa atttccatcc cggtttgttg ttcaaaatca
    30301 gtaacaaaac gctgtaagcg gccgatatca atgggagcat caacaccggc acgggtacaa
    30361 ccgctttggc ataatttctc cgtcgggcac actctggcac aaacggcacc gagggcatta
    30421 ttttcgcgaa ttgtctcggc agcgccttta aaattacgaa agtagattga gcgaataaat
    30481 ttccccggat cggtctgtgc cgggcaagcc tgactacagg gagcgtcgtg acataataaa
    30541 cagcgagagg cttctttaat cgccagtaac ggcgtaaaag ccggagtgag ttcatccaga
    30601 taattttgtt gcggcatgtg atcttcctta atgctatgag taattatggc gaacgctaaa
    30661 taaatattgc gtgctgatat taaggctatg cgcgtgggga aatttaagct gtgaaaggat
    30721 tcacaaatag ctgcggttca gaatgttata tccgcagctt attgagtgaa gtaataatta
    30781 ttctcaataa ttaacagaaa agagaaaata ttaacgatag aaaataagat aaacaatcat
    30841 gccgatagat aaaattccga agccaatata tgccaccata taagcgaaga agagtccggg
    30901 attttttgct cgccagacat acaccggttc cggtgcattt ttaacccgtt tatcaatcgc
    30961 gcctgcgcgc cagcctttcc atgcttcgcg gaaacaaaat atcgggataa ggaaaaaaac
    31021 ggcaacgaca aaaaactgct gtacatccat aactactttc cttgtttctt ttgtaattca
    31081 agtaactgtt cgggtgtaac ggcgggatac ccctgcgcat cttccggcac ctggtgcata
    31141 gccggaatag ggaccagcgg ccctaaaaaa cgcggttcac gtttaaaaat ataaaggtca
    31201 gccagcgcac cgaaacgggc ggcaaattcg cgaatacgta ctgacagcat atctttcggt
    31261 gacggtacgg cataacactg agcctgaatc cccatatgca gcgcaataaa taatgctcgc
    31321 tcacagtgga aacgttgggt gataataatg aaatcattag tatcgaaaac tttgcgtgta
    31381 cgcacgatgg aatccagcgt acgaaagcct gcgtaatcga gaacaatatc tgatgggtcg
    31441 acaccagcag cgattaaatc tttgcgcatg gtcatcggct cgttataact ttgcaatgcg
    31501 ttatcgccgc tcagtaatag ataatttacc ttaccgctgt tataggcatt aatcgctcct
    31561 tgaatgcggt agcgataata ctgattaata acgccggtac gataatattt tgctgtgccg
    31621 agcaccacgc cgacctggcg gtaggggaga tcctgcaatt cgtcgtagat ataaggcgcg
    31681 gttttccagc tcatccagcg atcgaggccg agcacagtca acagcagcaa gccgatcagg
    31741 actaacaggc tgaggaacac gcgctttaac atgcaggaga ctcgagctaa ggaaaatttc
    31801 catcaggcta ctttacccgc tggttttgcg caagaaacgg caaactgttt gccgggaatt
    31861 tcgacaccag gagagggctg gcaagccgga caaggcgttc acgccgcatc cggcaccagg
    31921 cttaagcgag aagcacgcgg tcgatactgt gacagccgag agctttcagc gttgcggagg
    31981 tggcatccca ctgaatcaac ggcgcatcgg ctttttctgc cagaaccgcg cgctggatat
    32041 ccgggtaatc ataacccttg agattcaaca gaattaacgc cccctgcaac ggcggcagag
    32101 tcgggttgaa tgcggcgttt tcagcgtagc tgccgctgaa aatacgaccg tctttacatt
    32161 ccagcgcgac accgcttggt gacttactgt aaggcatgtg cgaacggttt gccgctgcaa
    32221 tcgccgcctg agaaagcgca tcaccagtca gcgcatagcc gtgatcctgc tcgtccatca
    32281 gcagcgtttt aatctccaga tctttcggcc caaaggcatc cggcagatag tcacgcagcg
    32341 cgtgtgcctc gcggcccggc aaatgaatac gcagatccag accgctgttc agttcattca
    32401 taaactgacg gcagtgacca caaggcgtat agttaacggt gatggctgca agcgcttttt
    32461 caccactcaa ccaagcgtgg ctgatcgcgc tttgttcggc atgaacggtt tgctgcattg
    32521 ttgcaccaat aaattccata ttggcaccaa aataccaggt tccgctcaca ccgcgcgcaa
    32581 ttgcgccaac attaaaattc gacaatggcg tacgcgcaca ggccgccgcc agcggaagta
    32641 gtgcgaatgc cagcgcgtct tcgtccagtc ccgttgcgct cttcagcgat gagacttgct
    32701 ccccggtcaa caaagcgggg aagtacttgt ctgccagaat aggttccagt gcagattgca
    32761 agttatccgc aagttgggca aaagcggttt gaaaacgtgg atgcatggcg ttgcctcata
    32821 atcatgtaat gggataacag tttacgaacc cgttatggct ttatatgtga tctgaatctc
    32881 attaattatg caacacgtga aacttacatc aaaaatgcgc gacgtatcgc aaattttagc
    32941 ccattactgc cagaataatc gggaaaagga acggtgcgat cagcgaagta attatcccgc
    33001 ataacaccag cgctagcgaa ctaaatgcac cttcctgata atccagctcg gcgcagcgcg
    33061 ccgtaccgag ggcgtgcgag gcagtcccca ttgccagacc gcgcgcagct ttggtacgaa
    33121 tacgcatcgc attaagcaat gtatggccaa ataccgcgcc gaggatgccg acgaaaatca
    33181 cgcaaactgc gctgattgcc ggaataccgc caatactgcc gccaaccgcc attgcaatag
    33241 gcgtggtgac tgatttcggc aggattgacg cggcgatttc cggtgaagcg cccatcaaca
    33301 atgccacgga agtccccgtc accatcgcaa ccacgctgcc gataaaacag atggtgatga
    33361 tcgatttcca gcgcgcgcgg atctggtgta gctgctcata taaaggatag gccagcgcga
    33421 cgaccgccgg ttgcagcaga tcgtttagca cttcgctacc tttaaagtaa ctgtcgtaag
    33481 agatgccggt cagcattaaa aaaggaatga tcaccaccat tgctaccagc aacgggttaa
    33541 gcaggggaaa cttataccgt gccgccagtt tgcgggcggc aaaaaagaca atcaacgtca
    33601 gcggtaatga ccaccagata ttcgccatca ttcttctgat cctttctgac ctacgacttt
    33661 acgttcaccg tgtaccagtt gtgaactcca gctcaccacc agaaaaacca ccagcgtact
    33721 gacagcacag gaaaccacta ccgggccaaa ctgcgcgcgg agcaaatcaa aatattgcat
    33781 gacgcctacg ccaatcggca caaacaatag cgccatatag cgaatcagta cgtagcaccc
    33841 cggattgacc catttcgccg gaagaatttg caaggccagc aggacaaaca ggatcagcat
    33901 cccgatgatg ctgcccggaa tggttaccgg tagcagggaa gcaatgaaaa tgcctgcata
    33961 caggcaggca taaatcagga cgaaagcgcg taaatattgc cagataatgt tcagtgtctt
    34021 actcataatc aacaccccag atgagatgga ttcatcatac aattaattcg taaaacgtgc
    34081 taccgatcac attaagaaat cagcgcttat tttttcgcta cccggtatta cgcaatgagc
    34141 agcgaactca ggcatacatc aagcatagag agggattgca gtttgcagca atccctcttt
    34201 gcagaccttt aatccgctga ccataggcgc taacatagcg cctcattttt ttgcgggtga
    34261 tgatatgcgt gtgttactgg caccgatgga gggagtgctt gactctctgg tgcgtgaatt
    34321 gctgaccgaa gttaacgact acgatctgtg catcaccgag tttgtccgcg tggtggatca
    34381 actgctgccg gtaaaagtct ttcatcgcat ttgccctgag ctacaaaacg ccagccggac
    34441 accatctggt acgctggtgc gcgtgcagtt gttaggtcag ttcccacaat ggctggcaga
    34501 gaacgccgcc cgtgcggtgg agttaggttt ctggggcgtg gatctcaatt gcggctgccc
    34561 gtcgaaaacg gttaacggta gcggcggcgg ggcgacgtta ctcaaagatc ctgaactcat
    34621 ctaccagggg gcaaaagcga tgcgtgaagc tgtaccggcg catttgcctg tcagcgtgaa
    34681 agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa atcgccgatg cggttcaaca
    34741 ggctggcgct acggagctgg tggtgcatgg gcggacgaaa gagcagggtt accgcgcgga
    34801 gcatattgac tggcaggcga ttggcgagat tcgccagcgg ctgaatattc cggtgattgc
    34861 caacggtgaa atctgggact ggcagagcgc gcaacaatgc atggcgatca gcggctgcga
    34921 cgcagtgatg attggtcgcg gggcgctcaa tattcccaac ctgagccggg tggtaaaata
    34981 taacgaaccg cgaatgccgt ggccggaggt ggttgctttg ctgcaaaaat atacccgtct
    35041 ggaaaagcag ggcgataccg ggttatatca cgttgcgcgg attaaacagt ggttgagtta
    35101 tttgcgtaaa gaatacgatg aagcaacgga attatttcag catgttcggg tgttgaataa
    35161 ctcccctgat attgcaaggg ctattcaggc aattgatatc gagaaactct aaaaaccata
    35221 gtgatgtttt tgacaatgtg ttagctcttt atcaataggc cgcacggttg tttggtggcg
    35281 tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc acatggatgt gggctgaggg
    35341 cgcgttttac agggatgtta cctcgcgccc gacccggtag ccgtaaggga taagccgagg
    35401 gcaccgcgca gcggcgattt tgttcgccag agcccggggg ggcagggggc ggcggcgatt
    35461 ggtcgccccc tgcgcgctcc ttgcgccagt gattatatga agcaatgcac atgacaggag
    35521 cgcaacagga tgagagactg aaatattctc gttattttcg gatctgatga cattaattaa
    35581 ttttaaaata tcatcttaaa tacgccagtc accaccagta gcccaatcag gaaaataatc
    35641 aatacagccc agagtataat tttcattggc gttcctttat ttgtgagtgc tgaataagta
    35701 taggaaaaaa cgaagccctt tgccgttatc cataaacgat taagataaat cggaagatat
    35761 catttactta gtctatattt ttctgggtat gtcgttttat tgctactgat gtattgccac
    35821 gcgaaactaa aaaatatatc gcaacacacg gtaatctccg cgcaccgttt cttacctgat
    35881 tattccccca tgaatcgtga ttccttttat ccagccatcg cctgttttcc gctgttactg
    35941 atgctggccg ggtgtgcgcc tatgcatgaa acccgccagg cgttaagcca gcaaacgccc
    36001 gctgcacaag ttgacaccgc attacccacg gcgctgaaaa atggttggcc agacagccaa
    36061 tggtggctgg agtatcacga taatcagctc acttcattaa ttaacaatgc actacagaac
    36121 gcaccggata tgcaggtggc tgaacagcgc atttagctag ctgaagccca ggcgaaggca
    36181 gttgccactc aggatggtcc gcagatcgac ttttcggcgg atatggagcg gcaaaaaatg
    36241 tcggcagaag ggctaatggg gccgtttgct ctgaacgatc cggccgcagg tacgaccggt
    36301 ccgtggtaca ccaacggaac ttttggctta acggcgggct ggcatctcga tatctgggga
    36361 aagaatcggg cggaggttat tgcccgcctg ggtacggtta aagcacgggc ggcggaacgc
    36421 gagcaaaccc gccaattgct ggctggcagc gtagcccgcc tgtactggga gtggcaaacc
    36481 caggcggcgt taaacacggt cttgcagcaa atagaaaaag agcagaacac cattatcgcg
    36541 accgatcgcc agctatatca gaacgggatt acttcttcag ttgacggtgt ggaaaccgat
    36601 attaatgcca gcaaaacccg gcagcagctc aacgatgtcg cggggaaaat gaaaattatt
    36661 gaggcacggt taaatgcact cacaaatcac cagactaagt cattgaagct taaaccggtc
    36721 gcgttgccga aagtggcaag ccagcttcct gatgaactgg ggtactcctt actggcccgg
    36781 cgggcagatt tgcaggcggc gcactggtac gttgagtcat cgttaagcac tattgatgcg
    36841 gcaaaagcgg cattttatcc tgacatcaac ctgatggcct tcctgcaaca ggatgcgttg
    36901 cacttaagcg atctgttccg tcattccgcg cagcaaatgg gcgttacggc aggcctgacg
    36961 ctacctattt tcgatagtgg tcgtcttaac gccaatctcg atatcgcaaa agccgaaagc
    37021 aacttgtcta tcgccagcta caacaaagcg gtggttgaag cagtgaatga cgtggcgcgg
    37081 gcagccagtc aggttcagac actggcggag aaaaaccagc atcaggcgca aattgagcgc
    37141 gatgccttgc gtgtggtagg tcttgcgcag gcccgcttta acgcgggcat cattgctggt
    37201 tcccgcgtca gcgaagccag aatcccggcg ctgcgtgagc gggccaatgg cctgttattg
    37261 caagggcagt ggctggatgc ctccattcga ctcaccggtg cgctgggcgg gggttacaaa
    37321 cgctgatgat atgcgccttc tatacttaac gtttattcag cgttaagtgg agaactcgat
    37381 ggcataggtc gcgataatta ccgcttccga ttcggggatc ggcaaagagt gcgcgttatt
    37441 actggcgcag caggggtttg atattggcat tacctggcac tcagatgaag aaggggcaaa
    37501 agataccgcg cgtgaggtgg ttacccacgg cgtacgtgcg gagatcgtgc aactggatct
    37561 cggcaatctg ccagaaggtg cgcaggcgct ggagacactc atccagcgat ttgggcgtat
    37621 tgatgtgctg gtgaataatg cgggtgcaat gaccaaagcg ccgtttcttg atatggcttt
    37681 tgatgagtgg cgcaagattt ttaccgttga tgttgatggt gcatttttat gctcgcaaat
    37741 tgcggctcgt cagatggtga aataagggca gggcggtcgc atcatcaaca ttacgtcggt
    37801 acatgaacat acgccgctgc cggatgccag cgcctacaca gccgctaaac atgcgctcgg
    37861 cgggttaacc aaaacgatgg cgctggagtt ggtcaggcat aagattttgg tgaacgcagt
    37921 cgcgcctggg gcgatagcca cgccaatgaa cagcatggat gatagcgacg tgaagcccga
    37981 cgcggagcct tcgattcctc tacggcgttt tggcgcaacg catgagattg ccagcctggt
    38041 ggtgtggctt tgttcggagg gcgcaaatta cacgacaggg cagtcgttga tagtggatgg
    38101 cggctttatg ttggcgaatc cgcagttcaa cccagaatag cgcagtgagg tgcgtggata
    38161 tttcaatggg ttaggccaga taagacacca gcgtcgcatc aggcatggaa cacccggtgc
    38221 cggatgtgga gtgaatgctt atctgtggca actgctttac gcctgattat ccggcttttt
    38281 cttcccgcgc cgtttcagcc accagcgcac gcccaccacc agaaccacga ccagaatcaa
    38341 ccagacccag tgcttcaaat gctggtcgag attgtgcaac cacggcgcaa tcacctgacc
    38401 accagcgtaa ccaatagtgg taaaaatcaa cgcccaggca aatgcgccga gaatattcag
    38461 cggcagaaag attttcggcg gcagctggct ggcaccaatc agcgtcgggc caatcacccg
    38521 aaagccatac ataaagcgcg taccaatgac aaacagatac ggatggcgtt ggataagttt
    38581 ctgtgcccgc tcaattttat cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa
    38641 ccgccgcccg cacagataga gcacctggtc gccaatcatg ccaccaagcg ccacagaaag
    38701 taccaccagt gggaacttta atagcccctg atgtgccgca acgcctccca gcaaagtcac
    38761 ggtttcacct tccgccaggc taccgataac cagcgcggca taaccatatt gtgagatgag
    38821 tgtattgaga tccataggcc agtttgtctc cctttggttg cctgtaagca tacaccctgt
    38881 gaatcattca cgctgaaaaa ggacatacat cagacggtgc aaattacatg gatgaacaaa
    38941 aaataatcac aagctggctt atacttgaac tgtcaggttc atgaactcac aacaaggggg
    39001 cggtatgagc catgtctggg gactgttttc ccatcccgat cgtgaaatgc aggtgattaa
    39061 tcgcgaaaac gaaacgattt ctcaccatta cacccaccac gttttgctga tggcggcgat
    39121 tccggtgatt tgcgccttca ttggcactac acagattggc tggaattttg gcgatggcac
    39181 tatcctgaag ttatcctggt ttaccggact ggcgctggct gtcttgtttt acggcgttat
    39241 gctggctggg gttgcggtga tggggcgggt catctggtgg atggcgcgca attacccgca
    39301 gcgtccgtct cttgcgcact gcatggtctt cgcgggctac gttgcaactc cgctgttttt
    39361 aagcggtctg gtggcgcttt atccactggt ctggttgtgt gcgctggtcg gcacggtggc
    39421 gctgttttac accggctacc tgctttacct tggtattccg agcttcctga atatcaacaa
    39481 agaggaaggc cttagcttct ccagttcgac gctggcgatt ggcgtgctgg tactggaagt
    39541 gctgctcacc ctaaccgtta tactatgggg ctacggttat cgtttgttct agaaagttct
    39601 ttgacgttgc attgctggcg taaatgtcag caatgcagca tttactcacg attctccttt
    39661 gacgatcgca ctttttgctc gttatgatgg cgtcgctagc ctcagtaaat ccttactccg
    39721 gcggtgcgca acccgtgcgc gtgaaccact atctgaatgc tcatcatgcc gaaatttcga
    39781 gtttctttat ttagcttggc cctgatgctg gctgtgcctt ttgcaccgca ggccgttgcg
    39841 aaaacggcag ccgctaccac cgcttcacaa ccggaaattg cctccggtag cgcgatgatt
    39901 gttgatctga ataccaacaa agtgatctat tcgaaccacc cggatctggt gcgtccgatt
    39961 gcgtctatca gcaaattaat gaccgcgatg gttgtgctgg atgcacgact gccgctggat
    40021 gaaaaactaa aagtggatat cagccagacg ccggagatga aaggggtcta ttcgcgcgta
    40081 cgactgaata gcgaaatcag ccgtaaagat atgctgttgc tggcgctgat gtcttcagaa
    40141 aaccgcgcgg cggcaagcct tgcgcaccat tatcccggtg gttacaaagc ctttattaag
    40201 gcaatgaatg cgaaagcgaa atcgctcgga atgaacaaca cgcgctttgt tgaacctacc
    40261 ggattgtcgg tgcataacgt ttcaactgcc cgtgacttaa ccaaactgct cattgccagc
    40321 aaacaatatc cgttgatcgg gcagttaagt actacccggg aagatatggc aaccttctct
    40381 aacccgacgt atacgctgcc gttccgcaat actaatcatc tggtgtatcg cgataactgg
    40441 aatattcagt taaccaaaac cggctttacc aatgcggcgg gccattgtct ggtgatgcgt
    40501 acggttatca ataataaacc ggtggcgctg gtagtgatgg acgcgtttgg caaatatacc
    40561 cattttgccg atgccagccg cctgcgtacc tggattgaaa ccggtaaagt gatgcctgtg
    40621 ccggcagcag cgttgagcta taaaaaacaa aaagccgccc aaatggcggc ggcggggcag
    40681 acggcacaga acgattaagt gaattcggat ggcgatactc tgccatccgt aatttttact
    40741 ccacttcctg ccagtttttc cgtttactgg ttttaccgat ccccggattc atgctattgg
    40801 tcggatcatt ctcgcgatag aacttctgca acgtctccgg tgctttatac aaatgaccga
    40861 cgttatgctc ggcagggtac tgcgcgccgc gctgctgtag cagttccagc atctgttctt
    40921 ttaacgcatg cacatccacg ccttttttca ctatgtaatc ctgatggaag acatagcaca
    40981 taaaatggcc gtaatagagc ttgtgcacca gctggctgtc gatctccggc ggtaaatgct
    41041 cataccactc ggtgtcgtta cgccgcagag cgatatccag tgccagaatg tcttcgactt
    41101 catcggaatg caccgcctga taacgaattg ctgcgcccgc agcggcgaaa cggtgtaaaa
    41161 acgctttgct accttcctcc ggcgtacaga caaagaaatc gccctcggcc tgtttgaaat
    41221 aatccaccag ccacgatttg gcttcaccga cgccatcgcc tgccattttt aacagcagat
    41281 gatgctcgta tttatcgcgc cagtttttca tgcgcggcgg taaatggctg gggaacaggt
    41341 gaccgaattt ttgcattgca cggtcagtaa aatgcggacg gaagaatttc actttctcca
    41401 gcatcgcatc ggtgcgtccc ttgagattaa agaagaacgg catcttgtcg gtgcctaact
    41461 tatcaatcat caggaaggtg tctttgccgt atttttccgc aatatcgtag atatcccggt
    41521 gcatatattc cccggcaacc ggcagatttt cgaagttagc cagaatatga cggcggattt
    41581 cggtcagcac ttccggctgg ttggtgccga tataaaacac ctgctgattt ttttccgctt
    41641 cgaaggtatc aagacgtacc gcaaagacgg ccagcttccc ggcgcaacca gaagattcaa
    41701 ataaccgatc cggatcggcg ttataacgtg cgggcgtgtc ggcttcaata tcacgaacgc
    41761 ggtggacata gtcataatcg tgggcgtgac gaccatcgtg acgcacatcg tcatctttga
    41821 tgcgatcgtc atccagcttg ctaaggatct gctccggcgt ttcgcccaga tcaatcccca
    41881 gatggttcac cagcgtcagt ttgccatctt catctatacg cgcgaataac gacatttcgg
    41941 tatacgccgg gccacgttgc accagcgagc cgccggagtt gttacaaata ccgccgatga
    42001 ccgatgcgcc tatacacgat gatccaatca ccgaatgcgg ttcgcgtccc agcggtttga
    42061 gggctttttc cagcgaatag agcgtggtgc ccggataggc cagcacctgt tcgcccttgc
    42121 cgagaacgtg cagcttgtcg agacgcaggg tgctgatgat cacgatatcg cgatcatagt
    42181 cgttaccgtt tggcgtcgat ccttcggtca ggcctgtatt ggcagcctgc atcagaataa
    42241 ttttgtcggc ggtgacgcag gctttcagca cccgccacaa ttccagtagt gagccaggga
    42301 aaacgacagc cagcgcgtcg ccctgaccag aacggaagcc cttgcgatag cgggccgttt
    42361 ttgcgggatc ggtgagcagg tgtgaatgac cgaccagacg ggcaagttca ttcaaaaagg
    42421 ctttattatc agttgttgtc atggaagaca ttttccactc cttgtggtgg cgaaaaaata
    42481 tcacaaacta gaatagcgcc ttgtatgaca attcagcgag tgaatatttt gaaaattcag
    42541 acaataaact gagcattctt tttctctctc catcatgctt atggcaaact acggtttttg
    42601 caccattttc gctgccacgt cgggcaacaa aggaagaaaa atccatatga aatggctatg
    42661 ttcagtagga atcgcggtga gtctggtcct gcagccagca ctggcggatg atttattcgg
    42721 taaccatcca ttaacgcctg aagcgcggga tgcgttcgtc accgatctgc tcaagaaaat
    42781 gacggttgat gagaaaattg gtcagctgcg cttaatcagc gtaggcccgg ataatccgaa
    42841 agaggcgatc cgcgagatga tcaaagacgg tcaggttggg gcgattttca acaccgtaac
    42901 ccgtcaggat atccgcgcca tgcaggatca ggtgatggaa ttaagccgcc tgaaaattcc
    42961 tcttttcttt gcttacgacg tgctgcacgg tcagcgcacg gtgttcccga ttagcctcgg
    43021 tctggcctcg tcttttaacc tcgatgcggt gaaaacggtc gggcgtgtct ctgcttatga
    43081 agcggcagat gatggcctga atatgacctg ggcaccgatg gtcgatgtct cgcgcgatcc
    43141 gcgctgggga cgtgcttccg aaggttttgg cgaagatacg tatctcacct caataatggg
    43201 taaaaccatg gtggaagcga tgcagggtaa aagcccggca gatcgttact cggtgatgac
    43261 cagcgtcaaa cactttgccg catacggcgc ggtagaaggc ggtaaagagt acaacaccgt
    43321 cgatatgagt ccgcagcgcc tgtttaatga ttatatgccg ccgtacaaag cggggctgga
    43381 cgcaggcagc ggcgcggtga tggtggcgct gaactcgctg aacggcacgc cagccacctc
    43441 cgattcctgg ctgctgaaag atgttctgcg cgaccagtgg ggctttaaag gcatcaccgt
    43501 ttccgatcac ggtgcaatca aagagctgat taaacatggc acggcggcag acccggaaga
    43561 tgcggtgcgc gtggcgctga aatccggaat caacatgagt atgagcgacg agtactactc
    43621 gaagtatctg cctgggttga ttaaatccgg caaagtgacc atggcagagc tggacgatgc
    43681 tgcccgccat gtactgaacg ttaaatatga tatggggttg tttaacgacc catacagcca
    43741 tttggggccg aaagagtctg acccggtgga taccaatgcc gaaagccgcc tgcaccgtaa
    43801 agaagcgcgt gaagtggcgc gcgaaagcct ggtgttgctg aaaaaccgtc tcgaaacgtt
    43861 accgctgaaa aaatcggcca ccattgcggt ggttgggcca ctggcggaca gtaaacgtga
    43921 cgtgatgggc agctggtccg cagccggtgt tgccgatcaa tccgtgaccg tactgaccgg
    43981 gattaaaaat tccgtcggtg aaaacggtaa agtgctgtat gccaaagggg cgaacgttac
    44041 cagtgacaaa ggcattatcg atttcctgaa tcagtatgaa gaagcggtca aagtcgatcc
    44101 gcgttcgccg caagagatga ttgatgaagc ggtgcagacg gcgaaacaat ctgatgtggt
    44161 ggtggctgta gtcggtgaag cacaggggat ggcgcacgaa gcctccagcc ggaccgatat
    44221 cactattccg caaggccaac gtgacttgat tgcggcgctg aaagccaccg gtaaaccgct
    44281 ggtgctggtg ctgatgaacg ggcgtccgct ggcgctggtg aaagaagatc agcaggctga
    44341 tgcgattctg gaaacctggt ttgcggggac tgaaggcggt aatgcaattg ccgatgtatt
    44401 gtttggcgat tacaacccgt ccggcaagct gccaatttcc ttcccgcgtt ctgtcgggca
    44461 gatcccggtg tactacagcc atctgaacac cggtcgtccg tataatgccg acaagccgaa
    44521 caaatatact tcgcgttatt ttgatgaagc taacggagcg ctttatccgt tcggctatgg
    44581 tctgagctat accactttca ccgtctctga tgtgaaactt tctgcgccga ccatgaagcg
    44641 tgacggcaaa gtgacggcca gcgtgcaggt gacgaacacc ggtaagcgcg agggggccac
    44701 ggtagtgcag atgtacttgc aggatgtgac ggcttccatg agtcgccctg tgaaacagct
    44761 gaaaggcttt gagaaaatca ccctgaaacc gggcgaaact cagactgtca gcttcccgat
    44821 cgatattgag gcgctgaagt tctggaatca acagatgaaa tatgacgccg agcctggcaa
    44881 gttcaatgta tttatcggca ctgattccgc acgcgttaag aaaggcgagt ttgagttgct
    44941 gtaatgcttt agtaagggcg cgacgtttgc gccctttgcc tgatgttcat atcttaactt
    45001 gctgaatttt cgccctttgt aggccggata aggcgctcgc gccgcatccg gcaggaaacg
    45061 ccggaccgaa tatgcctctt aagtatttat ctgattctgc aactgaatcc ttccgctcag
    45121 gctaactctg tcattatcaa ctatgctttt ctcttaattc gctgaaaaca acagtaaaag
    45181 aatgaggaaa gcatcatgcc actcttaaag ctctgggcag gttcactggt tatgttggca
    45241 gccgtgagcc tgccgctaca ggcggcttcc cccgttaaag tcggttcaaa aatcgatacc
    45301 gaaggcgcgc tgctcggcaa catcatcttg caggtactcg aaagccacgg cgtaccgacg
    45361 gtgaataaag tgcaactcgg aacgactcct gtggggcgcg gggcgattac ttccgatgaa
    45421 ctggatatct atccggaata taccggcaat ggcgctttct tctttaaaga tgaaaacgat
    45481 gcagcgtgga aaaatgccgg gcagggctac gagaaagtca aaaagctcga tgcggagcac
    45541 aacaagttaa tctggctgac gcccgcgcct gcaaataaca cctggactat cgccgtgcgt
    45601 caggatgtgg cagagaaaaa caaactcact tcgcttgccg acctgagtcg ttatctgcaa
    45661 gagggcggca ccttcaaact ggcagcctcg gcagagttta tcgaacgcgc cgatgcgtta
    45721 cccacgtttg aaaaagccta tggctttaag ctcggtcagg atcagttgct gtcactggct
    45781 ggtggcgaca cggcggtgac gattaaagcc gctgcccagc aaacctctgg cgttaatgcc
    45841 gcaatggctt acggcactga cggtccggtc gcggcgctgg ggctgcaaac cttaagcgat
    45901 ccgcaaggcg tgcaacctat ctacgcgcct gcaccagtgg tgcgtgaatc ggtgttgaag
    45961 gagtatccgc aaatggcaca gtggctacag ccagtcttcg ccagcctcga tgcaaaaaca
    46021 ttgcagcaac tgaatgccag cattgcagtg gaaggactgg atgccaaaaa agtggctgcc
    46081 gactacctga aacaaaaagg gtgggcgaag taatttcccg tgacttatct acgtattaat
    46141 cctgttctgg cgctgctgtt gttgctgacg gcaatcgcag cggcgctgcc gtttatcagt
    46201 tacgcgccta atcgtttagt ctcgggtgaa gggcttcatc tctggcaact gtggccgcaa
    46261 acgatctgga tgctggtggg cgttggttgc gcctggctga cagcctgttt tgtccccgct
    46321 aaaaaaggca gcatttttgc actcattctg gcgcaattcg tcttcgtatt gttggtgtgg
    46381 ggagctggaa aggcggcgac ccaactggcg caaaatggca gtgcgctggc gcgtaccagc
    46441 ctcggcagag gtttctggct ggctgctgcg ctggcattgc tggcctgtag cgatgccatc
    46501 cgccgaatct ccacgcatcc gctgtggcgc tggttgttgc atatgcagat tgccattatt
    46561 ccgctgtggt tgctgtactc cggcacgctt aacgatctct cactaatgaa agaatacgcc
    46621 aaccgtcagg atgtgtttga cgacgcgctg gcacaacatc tgacgttgct gtttggtgcg
    46681 gtgctgcctg cgttagtgat tggtgtgccg ttgggcatct ggtgctactt ttccactgcg
    46741 cggcaggggg caattttttc tctgctcaat gtcattcaga ccgtgccttc ggtggcgctc
    46801 tttggcctgt tgattgcgcc gcttgccgcg ctggttacgg cctttccgtg gctggggaag
    46861 ctcggcatag caggaaccgg aatgacaccc gcactgattg cgctggtgct ctatgccttg
    46921 ctgccgctgg tgcgaggcgt ggtagtcggt ttgaaccaga tcccgcgcga tgtgctggag
    46981 agcgccagag cgatggggat gagcggggcg cagcgtttcc tgcatgttca gttaccgctg
    47041 gcgttaccgg tatttctgcg cagcctgcgg gtggtgatgg tgcaaactgt aggtatggcg
    47101 gtgattgcgg cgttaatcgg cgcaggcggt tttggtgcgc tggttttcca ggggctgcta
    47161 agcagcgcca ttgatttagt gttgctgggg gtgatcccgg taattgttct ggcggtgctt
    47221 accgacgcgc tgttcgattt gcttatcgca ctgctgaagg tgaaacgtaa tgattgaatt
    47281 tagccatgtc agcaaactgt tcggcgcaca aaaagccgtt aacgatctca atctcaattt
    47341 tcaggaaggg agtttttcgg tgctgattgg cacatctggc tccggcaaat ccaccaccct
    47401 gaaaatgatt aaccgcctgg tggagcatga cagcggagtg atccgctttg ccggagaaga
    47461 aattcgctcg ctgccagtac tggagttgcg ccgccggatg ggctatgcca ttcaatctat
    47521 tggcctgttc ccccactgga gcgtggcgca aaacatcgct accgtgccgc aattacaaaa
    47581 atggtcgcgg gcgcggattg acgatcgtat cgacgaatta atggcgctac tggggctgga
    47641 gtcaaatttg cgtgagcgtt atccgcatca gctttccggt ggtcagcagc aacgtgtggg
    47701 agtggcgcgt gcactggctg ccgatccgca agtcttactg atggatgaac cttttggcgc
    47761 actggacccg gtaacgcgcg gcgcgttgca acaagagatg acgcgcattc accgtttgct
    47821 ggggcgcact attgtgctgg tcactcatga tattgatgag gcgctacggc tggcagaaca
    47881 tctggtattg atggatcacg gtgaagtggt gcagcagggg aatccgctga cgatgctgac
    47941 tcgtccggcg aatgattttg tccgccagtt ttttggacgt agtgaactgg gggtgcacct
    48001 gctttcgtta cgtagcgtgg cggattacgt gcgtcgcgaa gaacgggcag aaggtgaggc
    48061 actggcagaa gagatgacgc tacgcgatgc gctctccctg tttgtcgcgc ggggatgcga
    48121 ggtgctgccg gtggtgaaca cgcagggcca gccttgcggc acgctgcatt ttcaggatct
    48181 gctggtggag gcgtaagcgt atgaagatgt tgcgcgatcc gctgttctgg ctcattgctc
    48241 tttttgtggc gctgattttc tggctgcctt acagccagcc gctgtttgct gccttgttcc
    48301 cacaactgcc acgacccgtt tatcagcaag aaagttttgc agctctggca ctggctcatt
    48361 tctggctggt gggaatttcg agtttgtttg cggtgatcat tggcacaggt gccggaattg
    48421 ctgttactcg cccgtggggc gcggaatttc gcccactggt ggaaactatt gccgccgttg
    48481 gacagacttt tccgcctgtc gcagtgctgg cgattgccgt tccggtgatc ggctttggtc
    48541 tgcaaccagc gattatcgcc ttgatccttt acggtgtgct gcccatcctg caggtgacac
    48601 ttgccgggct gggagcgatt gatcccagcg tgacagaagt tgcgaaaggt atgggaatga
    48661 gtcgaggtca gcgactgcgt aaggtcgagc taccgctggc ggctccggtg attctggcgg
    48721 gcgtgcgaac ttcggtgatt atcaacattg gtacggcgac gatcgcctca acggtagggg
    48781 ccagtacgct gggtacgccc atcatcatcg ggcttagcgg atttaatacc gcgtatgtga
    48841 tccagggggc gttactggtg gcactggcgg cgatcatcgc agaccgcctg tttgaaaggc
    48901 tggtgcaggc gcttagccag cacgcaaaat aaaggtataa cctgcgagca tgacgccacc
    48961 aattccgcct aacgccataa acaggaacag ggcgatgacc ccaattttag ctatgcgcat
    49021 aatgcactcc ttatgttaac gaaaggattg tacagtaaag cgcatttgtt aacgaatcat
    49081 taaataccga gtgggaaaat atcatggcct tgttcttgcc aactggtgag ttgctgctgt
    49141 tgggcggagg ttcgattttc accgcaccac accagcaatg tacggccttc gaatagttca
    49201 gggcgtagtt gattgagcga gtgggcgagc gaggacatca atgcgccatc cttgttgact
    49261 ggcaatccag ccctccagcc acagacgggt ggtatcctga atattccagc caaccaccag
    49321 cgcatcttta ccctgttttt tacgtgccga agccagacaa atggcgatgt agttgatcag
    49381 tacgccgtcg aggatcgcca gcagcgcctg gagagtcggt tgttggcact gaagccgtcg
    49441 gcgcagagga ataaacagat gtgtggtgag tgtctgggcg gggtaatcct gaccgcgctc
    49501 tttgatccac gttcgcaggc tatgtagatt gccg
//
LOCUS       DAZOZX010000016        48322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZOZX010000016 DAZOZX010000000
VERSION     DAZOZX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.016"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            187..480
                     /gene="yicS"
                     /locus_tag="ACHUPT_000873"
     CDS             187..480
                     /gene="yicS"
                     /locus_tag="ACHUPT_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR0818559.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACHUPT_000874"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACHUPT_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR0818560.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="ACHUPT_000875"
     CDS             complement(1922..2374)
                     /locus_tag="ACHUPT_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR0818561.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACHUPT_000876"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACHUPT_000876"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR0818562.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="ACHUPT_000877"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="ACHUPT_000877"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0818563.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACHUPT_000878"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACHUPT_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0818564.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACHUPT_000879"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACHUPT_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0818565.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACHUPT_000880"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACHUPT_000880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0818566.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACHUPT_000881"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACHUPT_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0818567.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACHUPT_000882"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACHUPT_000882"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0818568.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACHUPT_000883"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACHUPT_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0818569.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACHUPT_000884"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACHUPT_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0818570.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="ACHUPT_000885"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="ACHUPT_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0818571.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACHUPT_000886"
     CDS             complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACHUPT_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0818572.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="ACHUPT_000887"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="ACHUPT_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0818573.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="ACHUPT_000888"
     CDS             complement(15085..15582)
                     /locus_tag="ACHUPT_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0818574.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="ACHUPT_000889"
     CDS             complement(15579..15941)
                     /locus_tag="ACHUPT_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0818575.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="ACHUPT_000890"
     CDS             complement(15931..16278)
                     /locus_tag="ACHUPT_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0818576.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /gene="yidI"
                     /locus_tag="ACHUPT_000891"
     CDS             16387..16800
                     /gene="yidI"
                     /locus_tag="ACHUPT_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0818577.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="ACHUPT_000892"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="ACHUPT_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="ACHUPT_000893"
     CDS             complement(18324..20039)
                     /locus_tag="ACHUPT_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR0818578.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21100
                     /locus_tag="ACHUPT_000894"
                     /pseudo
     CDS             20206..21100
                     /locus_tag="ACHUPT_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(21097..21911)
                     /locus_tag="ACHUPT_000895"
                     /pseudo
     CDS             complement(21097..21911)
                     /locus_tag="ACHUPT_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21911..23527)
                     /locus_tag="ACHUPT_000896"
     CDS             complement(21911..23527)
                     /locus_tag="ACHUPT_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0818579.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23822..24496
                     /locus_tag="ACHUPT_000897"
     CDS             23822..24496
                     /locus_tag="ACHUPT_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0818580.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="ACHUPT_000898"
     CDS             complement(24535..26196)
                     /locus_tag="ACHUPT_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0818581.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="ACHUPT_000899"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="ACHUPT_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0818582.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="ACHUPT_000900"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="ACHUPT_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0818583.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="ACHUPT_000901"
     CDS             27650..27982
                     /locus_tag="ACHUPT_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0818584.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29216)
                     /locus_tag="ACHUPT_000902"
     CDS             complement(27984..29216)
                     /locus_tag="ACHUPT_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0818585.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29317..30417
                     /gene="cbrA"
                     /locus_tag="ACHUPT_000903"
     CDS             29317..30417
                     /gene="cbrA"
                     /locus_tag="ACHUPT_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0818586.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30378..31715)
                     /locus_tag="ACHUPT_000904"
     CDS             complement(30378..31715)
                     /locus_tag="ACHUPT_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0818587.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31790..32938)
                     /gene="dgoD"
                     /locus_tag="ACHUPT_000905"
                     /pseudo
     CDS             complement(31790..32938)
                     /gene="dgoD"
                     /locus_tag="ACHUPT_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32935..33552)
                     /gene="dgoA"
                     /locus_tag="ACHUPT_000906"
     CDS             complement(32935..33552)
                     /gene="dgoA"
                     /locus_tag="ACHUPT_000906"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0818588.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33536..34414)
                     /gene="dgoK"
                     /locus_tag="ACHUPT_000907"
     CDS             complement(33536..34414)
                     /gene="dgoK"
                     /locus_tag="ACHUPT_000907"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0818589.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34411..35100)
                     /gene="dgoR"
                     /locus_tag="ACHUPT_000908"
     CDS             complement(34411..35100)
                     /gene="dgoR"
                     /locus_tag="ACHUPT_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0818590.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35378..36034
                     /gene="yidX"
                     /locus_tag="ACHUPT_000909"
     CDS             35378..36034
                     /gene="yidX"
                     /locus_tag="ACHUPT_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR0818591.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36080..36892)
                     /gene="yidA"
                     /locus_tag="ACHUPT_000910"
     CDS             complement(36080..36892)
                     /gene="yidA"
                     /locus_tag="ACHUPT_000910"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0818592.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37006..37404)
                     /locus_tag="ACHUPT_000911"
     CDS             complement(37006..37404)
                     /locus_tag="ACHUPT_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0818593.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37644..40058)
                     /gene="gyrB"
                     /locus_tag="ACHUPT_000912"
     CDS             complement(37644..40058)
                     /gene="gyrB"
                     /locus_tag="ACHUPT_000912"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0818594.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40087..41160)
                     /gene="recF"
                     /locus_tag="ACHUPT_000913"
     CDS             complement(40087..41160)
                     /gene="recF"
                     /locus_tag="ACHUPT_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0818595.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41160..42260)
                     /gene="dnaN"
                     /locus_tag="ACHUPT_000914"
     CDS             complement(41160..42260)
                     /gene="dnaN"
                     /locus_tag="ACHUPT_000914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0818596.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42265..43668)
                     /gene="dnaA"
                     /locus_tag="ACHUPT_000915"
     CDS             complement(42265..43668)
                     /gene="dnaA"
                     /locus_tag="ACHUPT_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0818597.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43965..44045
                     /locus_tag="ACHUPT_000916"
     CDS             43965..44045
                     /locus_tag="ACHUPT_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818598.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44275..44415
                     /gene="rpmH"
                     /locus_tag="ACHUPT_000917"
     CDS             44275..44415
                     /gene="rpmH"
                     /locus_tag="ACHUPT_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0818599.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44432..44791
                     /gene="rnpA"
                     /locus_tag="ACHUPT_000918"
     CDS             44432..44791
                     /gene="rnpA"
                     /locus_tag="ACHUPT_000918"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0818600.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44755..45012
                     /gene="yidD"
                     /locus_tag="ACHUPT_000919"
     CDS             44755..45012
                     /gene="yidD"
                     /locus_tag="ACHUPT_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0818601.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45015..46661
                     /gene="yidC"
                     /locus_tag="ACHUPT_000920"
     CDS             45015..46661
                     /gene="yidC"
                     /locus_tag="ACHUPT_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0818602.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46767..48131
                     /gene="mnmE"
                     /locus_tag="ACHUPT_000921"
     CDS             46767..48131
                     /gene="mnmE"
                     /locus_tag="ACHUPT_000921"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0818603.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13066 c        12219 g        11057 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc ccccaccagt atgatggata tttccgttgg
    20581 tcagcaaagc gttatttata atggcgaaat ttataatcag gaactcaccg aagttgctga
    20641 gttaataact tcaccagaag caataaaaaa taatctggca gtcgcttttc tgacgaaaat
    20701 tatttatcag tggatttgtc ttatgtccgc agatggtaaa aaagatccac aacggcggca
    20761 aattgaaaaa ttaattgcca ctttacatgc cagtctgcaa caacgctgga gcgtagctga
    20821 tatggctgcc acgatcccct gtagcgaagc ctggttgcgt cgtctgtttt tacgctatac
    20881 cggcaagacg ccgaaagaat attacctcga tgcgcgtctg gatctggcgc tatcgctatt
    20941 aaagcaacaa ggaaactcgg ttggcgaagt cgctgatacg ctcaacttct tcgactcctt
    21001 tcatttcagc aaagccttta aacataaatt tggttatgcg ccgtcagccg tgctaaagaa
    21061 tacggaccag cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc
    21121 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
    21181 tttcgacaac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
    21241 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
    21301 gccgaccacc agtggttcag gccgtagtag cgcacgcgca tctgtttgcg atcttgcagc
    21361 ccaacaattt gcgccatccg gctttcaata ccgattggca tgtcacagat gttgaggatt
    21421 ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa ttgctgccgg gttggagtag
    21481 ttgagcatct aggcgtttgg tgaatatttt tccatataat ccaccagttc caggacgccg
    21541 ccaatggaac gcatgccgta cgctattccg ccgggtccgc aagtttcctg accaacaaca
    21601 ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg ggtacttgcc gacgcggatg
    21661 tgcgccatga caaaatcaac gtcgctgaac gccacttcag gatcggtggt gtaactaaac
    21721 gcaatgtccg gcgctttttc tttaaggatg actttacagg cttcggcaat cacttcctgc
    21781 cgcgcgccat cgttatcata aaatttcagt gcacgaagcg ggaaacggtc ctgattcgcc
    21841 aggagcatca acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac aaccactgag
    21901 aatttggtca ttatactgcc tccgtaatgg caacattttc tgctgattga tgagaattaa
    21961 ttaagctatc gagctgttca cgcaattggg atacatgcag accaatgatc acctgaatgg
    22021 catcgccact acggaagacg ccgtgcgctc ccagcttttt aaagacttcg tcatccagcg
    22081 tttgtgacat gtcatgcagt gcaatacgta gacgcgtcgc gcaattgtta atgctggaga
    22141 tattgccgac cccgcccagg gcttgcagga taccggcagc ctgatccagc tctttttttg
    22201 gctctgcagc ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag tagagtttca
    22261 cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg cagaatcagg gtgcggaaaa
    22321 ccacgaagta cagcagggta aagcacaacc caatggcgat ttgggtcagc atcatatccg
    22381 cgtggttgct gaacatcggg atccagtttt gcggtaaaac ctggtcaatc agacctccgc
    22441 ccatgttgcc caccacacca aagagataca ttacggtcga cattgaggcc gccagcacgg
    22501 cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt aaattccagc ggttcggtaa
    22561 ttcccaccag catggcggtt aaggttgcgg gaatcagcaa gcccgccact tttacccgat
    22621 tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc cacggcacca aagatttttg
    22681 agttaccgtg cagggcaaaa ccgccttccg ggaacaacga tttcagtggc tcggcgctca
    22741 gactgaactc ttgcagatgc tgcgcccagt acatctgaat gccgccttca acagctgccg
    22801 gaccaaagat aaacggtccg tagatgaagt ggtgtaaacc ggttgggatc agaatacgtt
    22861 cgaggaaggt gtaaacccac accccaagcg cacccgccga acgcaggaac gcttgcagag
    22921 attcaatccc catttgtact tttggccagc cgagcaatgt cagccaggca caggggatca
    22981 tcaccaggaa ggcgataatc accacataag acgtcccctg gaaaatgccg agaaaaaccg
    23041 gcagtttttt atcgaacaga cggttatgca gcgccgtcac aatgccggaa atgataattg
    23101 cgccgataat gctggtatcg agggttttaa tcccggccat cattgtcaga ccgctacctg
    23161 ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc ccaggtcatt cccatcgcat
    23221 tgatgaaata gttccaggtc aggaaactca ccatcaccgc cagacaagca cgtccctgcg
    23281 cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat cagcggcata ttacggaata
    23341 ccgtccaacc gccctcttca ataatgtgta cgatttgcgc gaataaactg ttcggatcgg
    23401 tcagtgattc cccgacaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcag cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaattcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtgcctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatgggt taacttgcgc ggttcgccgc cctgtggcga gataagccac
    28261 agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta
    28321 cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg atgggtaaag
    28381 gttaaacgtc gctgcacgac gccacgcggt ggcgcgggta gggttgtttc cgttccactt
    28441 aacggcgcat cacctgccga tttccagcta gcttcatctt gcggtaactc aacgataaat
    28501 agctccggca ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga
    28561 tttcctaccc atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg
    28621 ggcgtggttt tactcaccag cacacaccag tggctaccgc tgtattcacg cggatgttgc
    28681 ttttgtacgt tgaccgggcc aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc
    28741 ggatcgagtt gatgcattac atggtcgtta taggtaaagc tccccctttc accgttcggg
    28801 ctaaagacat gcacatggct gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata
    28861 tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg
    28921 aagtcgtaat gccatgtttc atcaggattt tcagggccgt gaataaagac atatttctct
    28981 gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc
    29041 tcgacctcgc ctgtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca
    29101 gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtatttgta
    29161 ttggtattgg tattggtgag caggtgatta cggggagcaa aggttatctg tttcatggcg
    29221 cggtcctgat acaaaacggt tcccgccatc atacttcaca aggacttcac tttcagacaa
    29281 tagtcttttg ccatcgtgat gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt
    29341 attggcctcg gcccggcagg gtcggcgttg gcacgaaagt tagccggcaa aatgcaggtg
    29401 atcgcgctgg ataaaaagca ccagtgcggt actgaaggtt tcagcaaacc ttgcggcggt
    29461 ctgctggcac cggacgcgca gcgatctttt attcgcgatg gactgacgct tcctgtcgat
    29521 gtgatcgcca atccacagat tttcagcgtc aaaactgtcg acgtcgccgc atcgctcacg
    29581 cgtaactacc agcgaagcta tatcaatatt aatcgccatg ctttcgactt gtggatgaaa
    29641 tcgctgatcc ccgccagcgt tgaggtttat cacgatagcc tgtgccgaaa aatctggcgt
    29701 gaggatgata aatggcatgt catttttcgt gcagacggct gggagcaaca tattaccgcc
    29761 cgctatctgg tcggtgccga tggcgcaaac tcgatggtgc ggcgacatct ctacccggat
    29821 catcaaatcc gtaaatatgt cgctatccag cagtggttcg cagagaaaca tccggtgccg
    29881 ttctactcct gcatctttga taatgcgata actgactgtt actcatggag tatcagcaaa
    29941 gatggttatt ttatctttgg cggtgcctat ccaatgaaag acggtcagac gcgtttcacg
    30001 acgctgaaag agaaaatgag cgcctttcag ttccagtttg gcaaagcggt gaaaagtgag
    30061 aaatgcacgg tgttgtttcc ctcacgctgg caggattttg tctgtggtaa ggacaacgcc
    30121 tttctgattg gtgaagcggc gggatttatc agcgccagct cgctggaggg gattagctat
    30181 gcgctggata gcgcagagat tctgcgtgcg gtgttcctga aacagccgga gaagagcaac
    30241 gccgcttact ggcgcgccac acacaaactg cgtttaaaac tcttcggcaa gatagtaaaa
    30301 agccgatgcc tgaccgcacc ggctttaaga aagtggatta tgcgcagtgg tgtggcgcat
    30361 attccacagt tgaaagatta tccaacgcgc ttcacatcac ccaccagcag gatgtaagag
    30421 agcgcgccaa tcaacgcgac ggcggagata taaaccagtg caggcgcgaa accgtaaccc
    30481 tgcgccaggt accccaccac cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta
    30541 aacacgccgc cggttaaacc aatcaggcgc atcggtgcca gagaagagac cagcgaccag
    30601 gtaatcgaag caaaaccgtt gccgaagaat gccagcgcca tcaggcacat aatcatcatc
    30661 ggatcgttgg tgtagttagc gcccataatg caggtggaga tcagcaagcc gcagataatc
    30721 ggcgttttac gcgcaaagcc cagtgaaaag cccttacgta ccagcagatc cgcgacccag
    30781 ccagagagca ggacgccgac aaacgccgcg aggaatggca ccgtggtcat aaagcctgct
    30841 ttcagcgccg tgattccttt ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac
    30901 cacagtgcag aagccaccgc aaattgccca agataaacgc cgatcagttt acgatggaac
    30961 accagtttcc agtctttggc tgttaacggc tgacgcgcct ctttcttcac cggcgcatca
    31021 ccatccacca gaccgccgcc atcacgaatg taatccagtt cagctttgct gatacctttg
    31081 gtcaggcgcg gcggctgata aaccttaaac caaatcagcg accagataat gccgatacca
    31141 ccagtgacaa tgaacaccca gtgccagctc aacatctcct gaatccagat cagcagcgga
    31201 gtcaaaaacg ccagaccgac aaactgacca gacgtataaa aaccaacggc ggaagcgcgt
    31261 tcatgttccg ggaaccagct ggtcaccatc cggttattgg tcgggaaagc gggcgcttcg
    31321 aaaataccgg ttatcgcacg cagaccaatt aatgacatta atcccgtggc aaagccttgg
    31381 aataaagtcg ccactgacca accaaatatc gcaataaaat aagtcacgcg agaacctacg
    31441 cgatctaaaa accaaccgcc ggggatctgg catagcgtat aaagccaggc gaaggccgaa
    31501 aatacatagc ccatttccgc tttcgtaatg ccgaactctt cctgaatatg ggcggaagcc
    31561 acggccaggt tagcgcggtc aacataacaa atgaccaccg taataaagat catcaccagc
    31621 gtcagataac gccgacgccc cggctttgct gcattaacgg gaatatccat agcgagcttt
    31681 ctccagattt tgggcatagc gaagccgctc accatgccct gtaatttaca gagggttata
    31741 tttttgtatt gctgttttag tgcccgatga ggggcttacg tggcaggaat taccactctg
    31801 ctacgctgtt atcttcatga cgccagagcg gattacgcca gtccggggca tttttactga
    31861 actcaataac tttagcttcg tcgatttcca cacctaagcc cggtttcgtt aacggtttaa
    31921 agaagccgcc aaccatgctg aagtcttctt tgtttttcac aaagtcgagt aactccgcgc
    31981 ctttgttgta atgaatgccc atactttgtt actgaagtac cgcgttatag gaaacgaagt
    32041 cgatatgcag gcaagccgcc agtgcaatcg gtccgaacgg acagtgcggc gcaagggtca
    32101 catcataggc ttctgccatt ccagcaattt tgtagcattc ggtaataccg cctgcatggg
    32161 agagatccgg ttgcagaatc gaaataccgc ctgcctccag cacgcgttta aaatcgaaac
    32221 gtgagaacat gcgctcaccc gccgccagtg gaatatgcgt ttgtgccgcc aatttcgggt
    32281 agtattcggc ttgttccgcc agcaccggct cttcaataaa cagcgggcga tacggctcca
    32341 gttctttaat cagcactttc gccattggcg cgctgacgcg accgtggaaa tcaagaccaa
    32401 actcaatctg attgccaaaa gcttcacgaa tttgtgccac ggtgttgact gccgcatcta
    32461 ccgcgcggga gttatcaatt agccccagtt cttcacaacc gttcagtttg aaggtatcga
    32521 agccgatttc gcgcagggtt ttaatgccgt cgataacatc cgccggacga tcgccgccga
    32581 cccaactgta ggctttaatt ttgtcgcgaa ccaggccgcc catcagttgc cagactggcg
    32641 cattcagcac tttgcctttg atatcccata acgcctggtc gatcccggcg atggcgctca
    32701 tcaggattgg accgccacga taaaatccgg cgcgatacat cacttgccat aagtcgttga
    32761 tgcgcgaagg atcctgacca atcaaatagt cacccagctc gtgaactgcc gcttccaccg
    32821 tacgggcgcg gccttcaatc acgggctcgc cccaaccgac cacgccttca tcggtttcaa
    32881 ttttcaggaa catccagcgg ggaggtaaac gatacgtggt aattttggtg attttcattg
    32941 cactgcctct cgatacgcct taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga
    33001 ctgcccggcg cgataaagat cgctgcctaa gcccgcccca acacaacctg cgtctatcca
    33061 ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag actgcgatgt ccggtggtaa
    33121 taccgctttt aacgctttga tgtattgcgg accaaaagcc gatgacggaa atattttcag
    33181 cgcctgcgcg cccgcttcga gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca
    33241 aacggtcatg ccgtagccca ccgcgcggcg gatcacttca ctatggatat tgggcgtaac
    33301 gatgagctga cagcccatcc tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc
    33361 tgcgccaatc aacgccttgt cgccatacgc atcaacgatg gcgggaatgc tctgctccca
    33421 ttgtggggaa ttcagcggga tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac
    33481 atgcgccagc gcctcgtcgg gcgtaatgcc gcgcaaaatg gcgatcagcg ggagtttagt
    33541 ttgccactgc atgagcgatg ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg
    33601 ccgccacgtc gcaacccatc gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc
    33661 cggcgacaag ggtgatggcg tgttgatggg tcacatagtc gcgcatactg gcaacctctg
    33721 cgccaatcaa caaaccagag agaaattcgc tgacctgttc gcggggaagt gttcccagca
    33781 catgcgaggc gcgaacttca aaaagctgcg gcaatatggc gggcgcatta aggccgcgct
    33841 caaggccagc cgcgaaggca tcggcagagt tttcctgcgg cggcaaacct gcgccaatca
    33901 atgagtgatt taacagtaaa tgatgtaatt caccggtcat cacggtgcga aaatcgttga
    33961 tttgctggct atcggcctgc acccatttgc aatgcgtacc gggcataaca taaagagagg
    34021 aaggagccag agctcgcgcg ccgatcaatt gtgtttcttc gccgcgcatc acattgtggt
    34081 tatcgtcatg agagacgcat aatccgggaa taatccagat attgtcgcca acagacgtta
    34141 attgttcgcc aatagacgaa aaacgggcag gaacagataa atacggagca actttccagc
    34201 cgacattgct gccgaccatt cctgccatca ccactggcgt attttcttca cgccagtcgg
    34261 tcgtgacttc tgctaacact gcagccggag attttccgtt caggcgcgtg acgcctgctt
    34321 ctgattgcct gctctccagg cagtggtcgc cctgataaag ccaggcgcgc agattggtcg
    34381 atccccagtc aattgcgatg tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga
    34441 gctggcaatc atggtaagcg ccgcctgctc tgccgcatcg ccgtcctgat gccgtatcgc
    34501 atcgaacagc gccttatgtt cctggagcgt ttgcggcatg ttggcctcat cgcccatcca
    34561 ggttcgttca aaaaccgccc gctgcagcga actgatcgca atgctaagtt gctgtaacac
    34621 cgggttatgc accgactgca gcaccgcctc gtggtagcga atatccgctt cgttaaacgc
    34681 ttcgcggtcc tgattgttgg caatcatctc gttcagcgcc gattcaatct gcgccagatc
    34741 gctggaggtc gcgcgctctg ctgcccaacg ggcaatcgcc ggttccacca gatttcgcac
    34801 ttcgctcatg gcactgataa gccgtgggtc gtagtcgttt tccagcaccc attgcagtac
    34861 gtcagtgtcg aggtaattcc actggttacg cggtgccaca aacgccccgc gataacgttt
    34921 catttcaatc agccgcttcg ccatcagcga acggaacacc tcacggatga tgttgcgcga
    34981 ggttgcaaac tcttcacaga gctccgcctc agccgcaagt ggcgagccag gtacgtattt
    35041 gccgtgaacg atctgtttac ccagcgtaat gacaatgcga tcggttttat tgagagtcat
    35101 ggagagtcct tgtgctctgt atgttcttct ctactttacc ccgatcgatg cataacgcgg
    35161 caactttgta gtaccagcgt gatgacgttc gcgtttgccg cgcgcgtaat gtagtacaaa
    35221 cttatattgt tgcgctacaa tttagatcaa aaaaagaaca acacataaaa aaatgacatg
    35281 cgtcgggcag aaatctgaaa agggatatca ggcgctaaac aggagggaaa gaagagtatg
    35341 ctttcaacgg cttagctact cgtttaaagg attaatcatg aagttgaatt ttaagggatt
    35401 ttttaaggct gccggtttat tcccactggc gctgatgctt tcaggctgta tctcgtatgc
    35461 tctggtttcc cataccgcaa agggtagttc aggaaagtat caatcgcagt cagacaccat
    35521 cactgggcta tcgcaggcaa aagatagtaa tggaacaaaa ggctatgttt ttgtagggga
    35581 atcgctggat taccttatca ctgatggtgc cgatgacatc gttaagatgc tcaatgatcc
    35641 agcacttaac cggcacaata ttcaggttgc cgatgacgca agatttgttt taaatgcggg
    35701 gaaaaagaaa tttaccggca caatatcgct ttactactac tggaataacg aagaagaaaa
    35761 ggcactggca acgcattatg gttttgcctg tggtgttcaa cactgtacca ggtcactgga
    35821 aaacctaaaa ggcacaatcc atgagaaaaa taaaaacatg gattactcaa aggtgatggc
    35881 gttctaccat ccatttaaag tgcgatttta tgaatactat tcacccagag gcattccgga
    35941 tggtgtttcc gcagcattac tgccagtgac tgttacactg gacatcatta ctgcaccgct
    36001 gcaatttctg gttgtatatg cagtaaacca ataatcagta agcgggcaaa cgcgtttatg
    36061 ctgtttgccc gcccacagat taattcagca catacttctc aatagcaaac gccacgccat
    36121 cttcaaggtt ggattttgtg acaaagttcg ccacttcttt caccgaagga atagcgttat
    36181 ccatcgccac accgacgcct gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa
    36241 tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc acacccgtac
    36301 ctttgttaac gcgtttatcg aggatttcga ggaagtacgg cgcacttttc agcacggtat
    36361 atttctcttt cacttcctgc ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat
    36421 caatcatcat cactttcagg aactgggtat tggggtccat tttctccgct tcgcagaaca
    36481 ccagcggaat tgtggcaacg aaggattcat gcaccgtgta gtagctgata tcgcggttag
    36541 cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga accgacttcg cgagagagtt
    36601 tttccaggaa acgatagtcg tcatagctga gagcggtttg cgccacggtg ctaccatcag
    36661 cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc tgttccatat
    36721 gcagctcttt caggtagttg tgcacacctg catacggacg acccgtcgtt agcacgacat
    36781 tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa atggtgtgat
    36841 cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc atgagttccc
    36901 aaggtagatt agttcctgac ccatgctaac gcgattccgc tcaaaaatca gtacaacacc
    36961 cgagggaaaa ggggatgcaa cgcgggttgc atgctcccca tttgcttagc ggaagagttt
    37021 ccctttcagc agttccatgc ctgcggaaag cagatcgtta ttggcttgtg ctgacacttc
    37081 accttgcggt gagagcgcat caataatctt cggcaattgc tctgccagta aactggaagc
    37141 cgtactggta tccacgccaa gtttttgccc gagatcggac accgcatttg tgccgagcgc
    37201 cgactccagt tgctcgccgc taactgattg atttctctgt tgattactca accaggttga
    37261 gagaatggcc cctaagccgc cactttgcag tttttccagc agcacctgaa tgccaccctg
    37321 ctcctcaacc caacttaaaa tcgcctgata tttccccgca tcgcctttca gaaaggcacc
    37381 gacaacttca tcaaaaagcc ccatgataat cacctgtaaa gcgttacgtg ttgacccaaa
    37441 aagtatagat ctgcgggatg ataattgcgg attgcagaaa taaaaagggc ggagatgatc
    37501 tccgcccttt tcttatagct tcttgccgga tgcggcgtga acgccttacc cggcctacaa
    37561 aatcatgcaa aatcaatata ttgcaggagt ctcgtaggac taataagcgt agcgcatcag
    37621 gcacgctcgc atggttaacg ccattaaata tcaatattcg ccgctttcag ggcgttctct
    37681 tcaataaacg cacgacgcgg ttctacggcg tcgcccatca gcgtggtgaa caactggtca
    37741 gcggcaatcg catccttaac ggtaacgcgc agcatacgac ggctttccgg gtccatggtg
    37801 gtttcccaca gctgttccgg gttcatctcg cccagacctt tataacgctg gatggagagg
    37861 ccacgacggg actctttcac cagccagtcc agcgcctgct cgaagctggc taccggctga
    37921 cgacgctcgc cacgttcgat aaacgcatct tcttccagca agccacgcag tttctcaccc
    37981 agcgtgcaga tacgacgata ttcgccaccg gtgataaact cgtgatccag cggatagtca
    38041 gtatccacac cgtgggtacg cacgcgaaca atcggctcga acaggttttg ttcggcattg
    38101 gtgtggacat caaacttcca ctggctgccg tgctgttctt tgtcgttcag ttcgctgacc
    38161 agcgcgttca cccagcgggt aacggtctgc tcatcagaaa ggtcagcttc cgtcaacgtc
    38221 ggctgataga taagctcttt cagcattgct ttcggataac gacgctccat gcggttgatc
    38281 attttctgcg tcgcgttgta ctcagatacc agtttctcta acgcttcgcc agccaatgcc
    38341 ggtgcactgg cgttggtgtg cagcgttgcg ccgtccagcg cgatagagat ctggtactga
    38401 tccatcgctt cgtcgtcttt aatgtactgt tcctgcttgc ctttcttcac tttgtacagc
    38461 ggcggctgag cgatgtagac gtgaccgcgt tcaacgattt ccggcatctg acgatagaag
    38521 aaggtcaaca gcagcgtacg aatgtgcgag ccgtcgacgt ccgcatcggt catgatgatg
    38581 atgctgtgat aacgcagttt gtccgggttg tactcgtcac gaccgatacc acagccaagc
    38641 gcggtgataa gcgtcgccac ttcctgagaa gagagcatct tatcgaagcg cgctttctcg
    38701 acgttaagga ttttaccctt cagcggcaga atcgcctggt tcttgcggtt acgcccctgc
    38761 ttcgcagagc cgcccgcgga gtccccttcc accaggtaca gttcggaaag cgccggatcg
    38821 cgttcctggc agtctgccag tttgcccggc aggccagcta agtcgagcgc acctttacgg
    38881 cgggtcattt cacgcgcgcg acgcgccgct tcacgggcac gggcagcatc gataattttg
    38941 ccaaccacga ttttcgcatc agttgggttt tccagcaggt attccgccag cagttcgttc
    39001 atctgctgtt caaccgccga tttcacctca gaagaaacca gtttgtcttt ggtctgggag
    39061 gagaatttcg ggtccggcac ttttacggaa acgaccgcaa tcaggccttc acgcgcatcg
    39121 tcaccggtgg cgctgacttt ggcttttttg ctgtagcctt ctttgtccat gtaggcattc
    39181 agggtacggg tcatcgccgc acggaagcct gccaggtgag taccgccgtc acgctgcgga
    39241 atgttgttgg taaagcagta gatgttttcc tggaagccat cgttccactg cagcgccact
    39301 tcaacgccaa taccgtcttt ttcggtggag aagtagaaga tatttgggtg gatcggcgtt
    39361 ttgttcttgt tcagatattc aacgaacgct ttgatgccgc cttcatagtg gaagtggtct
    39421 tctttgccgt cacgcttgtc gcgcagacga atggaaacgc cggagttgag gaacgacaac
    39481 tcacgcagac gtttcgccag aatttcatat tcgaactcgg tcacattggt gaaggtttcg
    39541 aggctgggcc agaaacgcac catggtgccg gttttttcag tctcgccggt aaccgccagc
    39601 ggagcctgcg gtacaccgtg ttcgtagatc tgacggtgaa ttttaccctc gcgctgaata
    39661 accagctcca gtttttgcga cagggcgttt actaccgaaa caccaacgcc gtgcagaccg
    39721 ccggacactt tataggagtt atcgtcaaat ttaccgcctg cgtgcagaac ggtcatgatc
    39781 acttccgccg ccgatacgcc ctcttccggg tgaataccgg tcggaatgcc gcgcccgtca
    39841 tcctgtacag agacagagtt atcggcgtga atggtgacga taatttcttt acagtgaccc
    39901 gcgagcgctt cgtcgatagc gttatctacc acctcgaata ccatgtggtg cagaccggtg
    39961 ccgtcatccg tgtcgccgat atacataccc gggcgcttac gcaccgcatc cagccctttc
    40021 aggactttga tactggagga gtcataagaa ttcgacatca acgtttctcg ctcatttata
    40081 cttgggttaa tccgttattt tacccttttc cacggtaaac atcttcgaat tttcgtccga
    40141 catgtctata acgtgttcag cactgatcgc gctgacaaag acctgtgatt gcgtcgcttt
    40201 taagcggctg gcaagcaacc cgcgacgctc atcatcaagc tcagaggcaa aatcatctat
    40261 caggtagaga caccgccgcc cgctttcacg agtgaggaac tctccttgcg ccagacgtaa
    40321 ggcgcacatc aacagcttaa gctgcccacg cgataaggta tcttccaccg gcgcaccgtc
    40381 ggcgcgaatg cgtaagtccg ctttatgcgg gccatgcgcg gtgtaggtta gctggcgatc
    40441 gcgttcaaaa ttacgttcca gcacctcagc gtactctgtc tctttctccc agccgcgctg
    40501 gaaagagaaa gtcagagaaa actcagggag aaattgctta caggtatcat ccatatcggc
    40561 cgcgataccg gcgctatact ccgcgcgcca ggtgctgatt tgctccgcca gcgggatcag
    40621 ttctttatcc cacgggcgta gctgttcgta acgagtcacc tggcgcagcg ccgcattgcg
    40681 ctgcttgagc aatcgcttga gattgctcca ggcggtgaaa aatccaggtt cgttgtgaaa
    40741 gcatccccag tcgaggaatg ctcttctgta tttggggccg ccgttgagta aagtaaaccc
    40801 ttctggcgtt atcaactgca tcggcatcag gtgcgccagt tccgcgactt tatgcccgtc
    40861 cgtaccgtca atacggactt tgctgtcgcc ctgtttgtct ttggttaagc caatcgccgt
    40921 ctcgcgctct tcgccctgta atcgcccgtg gagaacaaat gcctcctgct catggcgaat
    40981 gacgcgacca atctgcaaac tgcgaaacgc ccgaccatgg ccgagcgtat agatggcttc
    41041 cagcacgctg gttttgccac tgccgttggc acctaccaga aagttaaagc cgggagataa
    41101 agcgagatcc gcggtttcaa tattgcggaa atcgcggatc aacaagcggg tgagggacat
    41161 tacagtctca ttggcatgac aacataggca gccgactgtg atgcggcatc ttcaatctgc
    41221 acgctggaaa ccgaatcggt cagcatcatg cggacgtttt cgcatttcag cgcgttcaga
    41281 acatccagca catagctgac gttgaagccg atttccatct ccgcaccgct ataggtaacg
    41341 tcgaggatct cttccgcttc ttcctgttcc gggttgttgg cagtaatttt cagctggttt
    41401 tcgctgacat acaggcgcac accgcggaat ttctcgttag agagaatcgc cgcgcgagca
    41461 aacgcctgct tgagcagatc gcagcctgct tccagatgtt tgtccgggtt cttcggcaga
    41521 acgcgacggt aatccgggaa gcgaccatcc accagtttcg aggtgaaaat aaagtcgcca
    41581 acgtgggcac gaatgttatt gctgccaatc tgcacacgca gcagattgtc gccgccgtcg
    41641 agcatacgca tcagttcaat cacgccttta cgcggtacga tcaccgaatg gcttggcaaa
    41701 gattgaccaa ttggcattga acagaccgcc agacggtggc cgtcggttgc cacggtgcgc
    41761 agttcttcac cttcggtttc aaacagcata ccgtttaagt aatagcgaac atcctgatgc
    41821 gccatcgaaa actgggttgc ttcaatcaga cgcttcatcg tcgcctgcgg cagggtaaat
    41881 tcgacttcgc tctgccagtc atcgaggttc gggaaatccg ccgctggcag ggtagacagc
    41941 gaaaaacggc tacgcccgga gcgcaccagc atccgttcac cttccagctg cacggcaatt
    42001 tccgcgcctt caggcagacc acggcagata tcaaagaatt tgcgcgccgg aacggtcgtc
    42061 gctcctggct cgtgtggctg aaccagcgca acgcgtgcca ccatttccat ctcgagatca
    42121 gtaccggtca gcgacaacgt accgtcagca acctgtaaca gcagattacc gagaatcggt
    42181 agcgtaggac gaccacctaa cggaccgctc acctgttgta gcggttttaa taaatgctca
    42241 cgttctacgg taaatttcat aggtttacga tgacaatgtt ctgattaaat ttgaaaaatc
    42301 ttctttgata tcgtggctct cttcacgcaa ctgctcgatc ttacggcagg catgaagcac
    42361 cgtcgtgtgg tcacgcccac caaacgcatc gccaatctct ggcagactgt ggttagtcag
    42421 ctctttcgct agcgccatcg ccatctggcg cggacgcgcc accgagcggg atcgacgctt
    42481 ggaaaggaga tccgcgactt tgatcttgta gtactccgcc accgtcttct gaatattgtc
    42541 gatggtgacc agtttttcct gcaatgccag caagtcgcgc agcgcctcac gcacgaagtc
    42601 gatggtgatc gcccgtccgg taaagttggc gttagcaata acgcggttca gcgccccttc
    42661 cagctcacgt acgttagatc gtagacgctt ggcgataaag aacgccactt cacccggcaa
    42721 acgaatgtca ttttcgtcgg cctttttcat caggatcgcc acacgggttt ccagctctgg
    42781 cggttcgatc gccacagtca gtccccaacc gaagcgggat ttcaaacgat cctcaacgcc
    42841 gttgatctct ttcggatagc gatccgaggt gagaatgatc tgttgattac cttccagcag
    42901 ggcgttgaag gtgtggaaaa actcttcctg agatcgttct ttattagcaa aaaactgaat
    42961 atcgtcgatc agcagtgcat ctacggaacg gtagtagcgt ttaaactctt cgatcgcgtt
    43021 gttttgcagg gctttaacca tgtcctgaac aaagcgctcg gagtgcatat aaaccacttt
    43081 ggcattcggc ttgcgcgcca taatgccgtt acccaccgca tgcagcaggt gagttttacc
    43141 cagacccgtg ccgccataaa ggaacaacgg gttataggca ccgccagggt tatccgccac
    43201 ctggcaagcc gccgcgcgcg ccagttggtt agatttacct tcaacgaagt tatcaaacgt
    43261 gtgtttgacg tttacgttag aacgataggt cggttctgcc ggggccggga cgttatccca
    43321 acctgagcgc gtagaaggcg cagcacgttg cggctgcgtt tgcgccacct gtgcaggggc
    43381 cgcgacgttg ctcgtcactg ccgcttgtgg cgtttgcgtc accggtttgg tgccgacttc
    43441 aaaacgcagc tgtggggcat ccgctccgca gaaactggtt agcagtccat tgatattatt
    43501 aaggtacttg tcccgtaccc aatcgaggac aaaacggttt ggcgcgtaca gggccagcgt
    43561 gttatcgctc agttccgcct gcaatgggcg tatccacata ctgaattctg tggctggtaa
    43621 ctcatcctgc aatcgggcaa gacactgctg ccaaagcgaa agtgacacgg cggactccac
    43681 tcgaacaaaa gtcgataatg actaaggctg aaacattcat gattgttgac gtacgtcgaa
    43741 aagaccctgc ttgagggtga cgcacgaacc gctgtctgcg gttatatgcc cgatcaagat
    43801 cctgcaaaac gatcgggacc gcggatcata gcctaaactg cgcaagagat cttctgtttc
    43861 tcacagattt tccctattta tccacaggac tttccagaac tcgctaagtg taaacgatcc
    43921 tgccgcgagg cgggcacgat ttacgccgca tattggaaaa tttaatgacc atagacaaaa
    43981 attggcttaa tcgatctaat aaagatccag gacgatcctt gcgctttacc catcagcccg
    44041 tataatcctc cacccggcgc gccatgctgg tttccactgg tgtgaggtcg tacattttcc
    44101 ctgcgaaaag gtgcggaaaa gcgcggtaaa taaggaaaga gaattgactc cggtgtgtac
    44161 aattattaca atccggcctc tttaatcacc catggcttcg gtgtccatcg tttcattttt
    44221 cggcggatat ccaataaagc cattgaattt attcaagttt aggtagaaat cgccatgaaa
    44281 cgcacttttc aaccgtctgt actgaagcgc aaccgttctc acggcttccg tgctcgtatg
    44341 gctactaaaa atggtcgtca ggttctggca cgtcgtcgtg ctaaaggccg cgctcgtctg
    44401 accgtttcta agtaataaag ctaacccctg agtggttaag ctcgcatttc ccagggagtt
    44461 acgcttgtta actcccagtc aattcacatt cgtcttccag cagccacaac gggctggcac
    44521 gccgcaaatt accattctcg gccgcctgaa ttcgctgggg catccccgta tcggtcttac
    44581 agtcgccaag aaaaacgttc gacgcgccca tgaacgcaat cggattaaac gcctgacgcg
    44641 tgaaagcttc cgtctgcgcc aacatgaact cccggctatg gatttcgtgg tggtggcgaa
    44701 aaaaggggtt gccgacctcg ataaccgtgc tctctcggaa gcgttggaaa aattatggcg
    44761 ccgccactgt cgcctggctc gcgggtcctg atagccctca ttcgggtcta tcaacgcctg
    44821 attagtccgc tactcgggcc gcattgtcgt ttcactccaa cctgttcaag ctacggaatt
    44881 gaggcattgc gcaggtttgg agtgataaaa ggcagttggt tgacggtgaa acgcgtatta
    44941 aaatgccacc ctttacaccc tggtggtgac gatcccgtcc cgcccggacc atttgatacc
    45001 agagaacact aacgatggat tcgcaacgca atcttttagt catcgctttg ctgttcgtgt
    45061 ctttcatgat ctggcaagcc tgggagcagg ataaaaaccc gcaacctcag gcccaacaga
    45121 ccacgcagac aacgaccacc gcagcgggta gcgccgccga ccagggcgta ccggccagtg
    45181 gccaggggaa actgatctcg gttaagaccg acgtgcttga tctgaccatc aacacccgtg
    45241 gtggtgatgt tgagcaagct ctgctgcctg cttacccgaa agagctgaac tctacccagc
    45301 cgttccagct gttggaaact tcaccgcagt ttatttatca ggcacagagc ggtctgaccg
    45361 gtcgtgatgg cccggataac ccggctaacg gcccgcgtcc gctgtataac gttgaaaaag
    45421 acgcttatgt gctggctgaa ggtcaaaacg aactgcaggt gccgatgacg tataccgacg
    45481 cggcaggcaa cacgtttacc aaaacgtttg tcctgaaacg tggtgattac gctgtcaacg
    45541 tcaactacaa cgtgcagaac gctggcgaga aaccgctgga aatctcctcg tttggtcagt
    45601 tgaagcaatc catcactctg ccaccgcatc tcgataccgg aagcagcaac ttcgcactgc
    45661 acaccttccg tggcgcggcg tactccacgc ctgacgagaa gtatgagaaa tacaagttcg
    45721 ataccattgc cgataacgaa aacctgaaca tctcttcgaa aggtggttgg gtggcgatgc
    45781 tgcaacagta tttcgcgacg gcgtggatcc cgcataacga cggtaccaac aacttctata
    45841 ccgctaatct gggtaacggc atcgtcgcta tcggctataa atctcagccg gtactggttc
    45901 agcctggtca gactggcgcg atgaacagca ccctgtgggt tggcccggaa atccaggaca
    45961 aaatggcagc tgttgctccg cacctggatc tgaccgttga ttacggttgg ttgtggttca
    46021 tctctcagcc gctgttcaaa ctgctgaaat ggatccatag ctttgtgggt aactggggct
    46081 tctccattat catcatcacc tttatcgttc gtggcatcat gtacccgctg accaaagcgc
    46141 agtacacctc catggcgaag atgcgtatgc tgcagccgaa gattcaggca atgcgtgagc
    46201 gtctgggcga tgacaaacag cgtatcagtc aggaaatgat ggcgctgtac aaagctgaga
    46261 aggttaaccc gctgggcggc tgcttcccgc tgctgatcca gatgccaatc ttcctggcgt
    46321 tgtactacat gctgatgggt tccgttgaac tgcgtcaggc accgtttgca ctgtggatcc
    46381 acgacctgtc ggcacaggac ccgtactaca tcctgccgat cctgatgggc gtaacgatgt
    46441 tcttcattca gaagatgtcg ccgaccactg tgaccgaccc gatgcagcag aagatcatga
    46501 cctttatgcc ggtcatcttc accgtgttct tcctgtggtt cccgtcaggt ctggtactgt
    46561 actatatcgt cagcaacctg gtaaccatta ttcagcagca gctgatttac cgtggtctgg
    46621 aaaaacgtgg cctgcatagc cgcgagaaga aaaaatcctg attcggtgag ttttcgctaa
    46681 aataagggcg gtcagttgac cgcctttttt cttttcgtag ggcggataag caccgcgcat
    46741 ccgccacaca aagcaacagg aacatcatga gcgataatga cactatcgta gcccaggcca
    46801 cgcctccggg acgtggcggc gttggcatcc tgcgcatctc cggcttcaaa gcccgtgaag
    46861 ttgccgaaac cgtgctgggt aaactgccta agccgcgcta cgccgattat cttccgttta
    46921 aagacgccga cggcagcgtg ctcgatcagg ggattgcgct atggttccct ggcccgaact
    46981 cgttcaccgg cgaagatgtg ctggaactgc aaggtcatgg cggtccggtg atcctcgacc
    47041 tgctgttaaa acgcattctg accattcccg gcctgcggat tgctcgccct ggtgagtttt
    47101 ccgaacgcgc gtttcttaac gataaacttg acttagccca ggccgaggcg attgccgatc
    47161 ttatcgacgc cagttcggaa caggcggccc gttcggcact taactcgctg caaggcgcat
    47221 tctccgcacg ggttaatcat ctggtagaag ccctcaccca cttgcgcatt tacgtcgaag
    47281 cggcaattga tttccctgat gaagagatcg atttcctctc cgacggcaaa attgaagccc
    47341 agcttaatga cgtgatggcc gatctcgatg cagtgcgtgc tgaagcacgt cagggtagtt
    47401 tgttgcgcga agggatgaaa gtagtgattg ccggacgtcc taatgccggt aaatcgagcc
    47461 tgttaaacgc gctggcgggc cgtgaagcgg caatcgtaac cgatatcgcc ggaaccacgc
    47521 gtgacgtgct gcgtgagcat atccacattg acggaatgcc gctgcatatc atcgacactg
    47581 ccgggctacg tgaggccagt gacgaagtgg aacgtattgg tatcgagcgc gcgtggcagg
    47641 aaattgaaca ggccgaccgc gtgctgttta tggtcgatgg caccacgaca gacgccgttg
    47701 atccggcaga gatctggccg gaatttatcg cccgtctgcc cgcgaaactg ccgatcaccg
    47761 tggtacgcaa taaagccgat atcaccggcg aaacgctggg aatgagtgaa gtgaacggtc
    47821 acgcgttaat tcgtctctcg gcaaggactg gtgaaggcgt ggacgtgctg cgtaaccatc
    47881 tcaaacagag catgggcttt gacaccaata tggaaggcgg cttcctggcg cgccgtcgcc
    47941 acttacaggc gctggaacag gcagcggagc atttgcagca aggtaaagcg caactgttgg
    48001 gagcctgggc aggtgaactg ctggcggaag agttgcgcct ggcacagcag aacttaagcg
    48061 aaatcaccgg ggaatttact tcagacgacc tgctggggcg gattttctcc agcttctgta
    48121 ttggtaagta accgcgctta cgaagccgta ttctgactgt cagatgcggc ttcgcttcat
    48181 tgttagcact cctgttatgc ctcaaccctt ttttaaacat taaaattctt acgtaattta
    48241 taatctttaa aaaaagcatt taatactgct ccccgaacga ttgtgattcg attcacattt
    48301 aaacaatttc agaatagaca aa
//
LOCUS       DAZOZX010000017        46539 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZOZX010000017 DAZOZX010000000
VERSION     DAZOZX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.017"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            361..486
                     /gene="timP"
                     /locus_tag="ACHUPT_000922"
     CDS             361..486
                     /gene="timP"
                     /locus_tag="ACHUPT_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0818604.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(518..1294)
                     /gene="sseB"
                     /locus_tag="ACHUPT_000923"
     CDS             complement(518..1294)
                     /gene="sseB"
                     /locus_tag="ACHUPT_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0818605.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1436..2719)
                     /gene="pepB"
                     /locus_tag="ACHUPT_000924"
     CDS             complement(1436..2719)
                     /gene="pepB"
                     /locus_tag="ACHUPT_000924"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0818606.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2778..2978)
                     /gene="iscX"
                     /locus_tag="ACHUPT_000925"
     CDS             complement(2778..2978)
                     /gene="iscX"
                     /locus_tag="ACHUPT_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0818607.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2990..3325)
                     /gene="fdx"
                     /locus_tag="ACHUPT_000926"
     CDS             complement(2990..3325)
                     /gene="fdx"
                     /locus_tag="ACHUPT_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0818608.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3327..5177)
                     /gene="hscA"
                     /locus_tag="ACHUPT_000927"
     CDS             complement(3327..5177)
                     /gene="hscA"
                     /locus_tag="ACHUPT_000927"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0818609.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5194..5709)
                     /gene="hscB"
                     /locus_tag="ACHUPT_000928"
     CDS             complement(5194..5709)
                     /gene="hscB"
                     /locus_tag="ACHUPT_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0818610.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5805..6128)
                     /gene="iscA"
                     /locus_tag="ACHUPT_000929"
     CDS             complement(5805..6128)
                     /gene="iscA"
                     /locus_tag="ACHUPT_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0818611.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6145..6531)
                     /gene="iscU"
                     /locus_tag="ACHUPT_000930"
     CDS             complement(6145..6531)
                     /gene="iscU"
                     /locus_tag="ACHUPT_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0818612.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6559..7773)
                     /gene="iscS"
                     /locus_tag="ACHUPT_000931"
     CDS             complement(6559..7773)
                     /gene="iscS"
                     /locus_tag="ACHUPT_000931"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0818613.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7885..8373)
                     /gene="iscR"
                     /locus_tag="ACHUPT_000932"
     CDS             complement(7885..8373)
                     /gene="iscR"
                     /locus_tag="ACHUPT_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0818614.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8643..9383)
                     /gene="trmJ"
                     /locus_tag="ACHUPT_000933"
     CDS             complement(8643..9383)
                     /gene="trmJ"
                     /locus_tag="ACHUPT_000933"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0818615.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9502..10305
                     /gene="suhB"
                     /locus_tag="ACHUPT_000934"
     CDS             9502..10305
                     /gene="suhB"
                     /locus_tag="ACHUPT_000934"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0818616.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10450..11304
                     /locus_tag="ACHUPT_000935"
     CDS             10450..11304
                     /locus_tag="ACHUPT_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0818617.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11495..12775
                     /gene="csiE"
                     /locus_tag="ACHUPT_000936"
     CDS             11495..12775
                     /gene="csiE"
                     /locus_tag="ACHUPT_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0818618.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12767..13906)
                     /locus_tag="ACHUPT_000937"
     CDS             complement(12767..13906)
                     /locus_tag="ACHUPT_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0818619.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14066..14956)
                     /gene="hcaR"
                     /locus_tag="ACHUPT_000938"
     CDS             complement(14066..14956)
                     /gene="hcaR"
                     /locus_tag="ACHUPT_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0818620.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPDIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15092..16453
                     /gene="hcaE"
                     /locus_tag="ACHUPT_000939"
     CDS             15092..16453
                     /gene="hcaE"
                     /locus_tag="ACHUPT_000939"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0818621.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16450..16968
                     /gene="hcaF"
                     /locus_tag="ACHUPT_000940"
     CDS             16450..16968
                     /gene="hcaF"
                     /locus_tag="ACHUPT_000940"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0818622.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16968..17288
                     /locus_tag="ACHUPT_000941"
     CDS             16968..17288
                     /locus_tag="ACHUPT_000941"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0818623.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17285..18097
                     /gene="hcaB"
                     /locus_tag="ACHUPT_000942"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17285..18097
                     /gene="hcaB"
                     /locus_tag="ACHUPT_000942"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0818624.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18107..19324
                     /gene="hcaD"
                     /locus_tag="ACHUPT_000943"
     CDS             18107..19324
                     /gene="hcaD"
                     /locus_tag="ACHUPT_000943"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0818625.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            19421..19843
                     /locus_tag="ACHUPT_000944"
     CDS             19421..19843
                     /locus_tag="ACHUPT_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0818626.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19891..20763)
                     /locus_tag="ACHUPT_000945"
     CDS             complement(19891..20763)
                     /locus_tag="ACHUPT_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0818627.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20775..21869)
                     /locus_tag="ACHUPT_000946"
     CDS             complement(20775..21869)
                     /locus_tag="ACHUPT_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0818628.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21902..22900)
                     /locus_tag="ACHUPT_000947"
     CDS             complement(21902..22900)
                     /locus_tag="ACHUPT_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0818629.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22925..24436)
                     /locus_tag="ACHUPT_000948"
     CDS             complement(22925..24436)
                     /locus_tag="ACHUPT_000948"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0818630.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24459..25441)
                     /locus_tag="ACHUPT_000949"
                     /pseudo
     CDS             complement(24459..25441)
                     /locus_tag="ACHUPT_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(25538..28819)
                     /locus_tag="ACHUPT_000950"
                     /pseudo
     CDS             complement(25538..28819)
                     /locus_tag="ACHUPT_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            28937..30130
                     /locus_tag="ACHUPT_000951"
     CDS             28937..30130
                     /locus_tag="ACHUPT_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR0818631.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30328..31581)
                     /gene="glyA"
                     /locus_tag="ACHUPT_000952"
     CDS             complement(30328..31581)
                     /gene="glyA"
                     /locus_tag="ACHUPT_000952"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0818632.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31908..33098
                     /gene="hmpA"
                     /locus_tag="ACHUPT_000953"
     CDS             31908..33098
                     /gene="hmpA"
                     /locus_tag="ACHUPT_000953"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0818633.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33143..33481)
                     /gene="glnB"
                     /locus_tag="ACHUPT_000954"
     CDS             complement(33143..33481)
                     /gene="glnB"
                     /locus_tag="ACHUPT_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0818634.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33542..34876)
                     /gene="glrR"
                     /locus_tag="ACHUPT_000955"
     CDS             complement(33542..34876)
                     /gene="glrR"
                     /locus_tag="ACHUPT_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0818635.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34866..35579)
                     /gene="qseG"
                     /locus_tag="ACHUPT_000956"
     CDS             complement(34866..35579)
                     /gene="qseG"
                     /locus_tag="ACHUPT_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0818636.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35744..37171)
                     /gene="qseE"
                     /locus_tag="ACHUPT_000957"
     CDS             complement(35744..37171)
                     /gene="qseE"
                     /locus_tag="ACHUPT_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0818637.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37729..41616)
                     /gene="purL"
                     /locus_tag="ACHUPT_000958"
                     /gene_synonym="purI"
     CDS             complement(37729..41616)
                     /gene="purL"
                     /locus_tag="ACHUPT_000958"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0818638.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41875..43431
                     /gene="mltF"
                     /locus_tag="ACHUPT_000959"
     CDS             41875..43431
                     /gene="mltF"
                     /locus_tag="ACHUPT_000959"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0818639.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43428..43931)
                     /gene="tadA"
                     /locus_tag="ACHUPT_000960"
     CDS             complement(43428..43931)
                     /gene="tadA"
                     /locus_tag="ACHUPT_000960"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0818640.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43989..44624)
                     /gene="yfhb"
                     /locus_tag="ACHUPT_000961"
     CDS             complement(43989..44624)
                     /gene="yfhb"
                     /locus_tag="ACHUPT_000961"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0818641.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44833..45681
                     /locus_tag="ACHUPT_000962"
     CDS             44833..45681
                     /locus_tag="ACHUPT_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0818642.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46020..46539)
                     /locus_tag="ACHUPT_000963"
                     /pseudo
     CDS             complement(46020..46539)
                     /locus_tag="ACHUPT_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11070 a        12794 c        11700 g        10975 t
ORIGIN      
        1 atattactta aacatgtaaa tttactttcc tttaaaaaac aaaaaaccgc caaaatcagg
       61 cggttatttg ttgctggtcc ggttcgcggc ctttccagca ggttgtatta ccgtagtaat
      121 gcaagcgcgt ctcagcggag acaatactcg ccagtaactc tctttttgtc aagcaaaaga
      181 gagttattat tgttctgtta gtgtattatc cactgcggcc ctttccgccg tctcgcaagc
      241 gggcgctggc ttaaggaaag gatgttccgc gaccgttaga gcaagcgcgt ctcagcgctt
      301 gctatcgcgg caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat ggaccgcaca
      361 atgaagatac ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt ttcccaagta
      421 aataataacc ggagtctctc cggcgacaat ttactggtgg ttaacaacct tcagagcagc
      481 aagtaagccc gaatgccgcc ctttagggcg gcatatttta gattatccgg ttttgtttaa
      541 agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg cgcaatatgt tcggtaataa
      601 agtggctaat tcctttttcc ccttttttca cctgacaaat atcgattggt tcatcgccag
      661 gtaatgtatc ggtcgctaca cttcccgtcg cctgaataat ttcttcgata tcaccatcgg
      721 cttcaatgcc aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt gaacaaataa
      781 aagcacgctt caccggctta atggttttaa ataaggtggt gagtgaatca atcatttgtg
      841 ctggcggctc tgcgacttcc gataatatca gcgattcacc gccttccagg atttcctggc
      901 tgctcagcgg atttccctct tcaccaatca acaaactgat ttcacgcggc ataaattctt
      961 taccggtcgg cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc tcaaacaatg
     1021 tgcgaacggg cattacgaca aatgcctgtt cgtcttcaac cgcctgttga agcgcttcta
     1081 acgaggtgaa aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc tgtaaatcaa
     1141 gcgcactatc ttcaaccaca gcctcgccct gcgccgccgt accaggcacc cagacggtgg
     1201 attccagtag agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct gctttttcca
     1261 gcaggtcttc aagttcgttt tttgtttcgg acataagaac cacaattcat tcaacgttcg
     1321 gggcgcaaat gcctgatgcg ctacgcttat caggcctaca agggattcgc aatttgttga
     1381 atttgcagaa tttgtaggcc ggataaggcg tttacgccgc atccggcata ttagtttacg
     1441 ccgttaacag attagctatc gtgcgcacac caagtcccgt agcgcccgca gaccactgtt
     1501 caaccggcgc tttacggtaa gtcgccgagc agtcgatatg cagccagcct tgctgatagt
     1561 tctcaacaaa gtgcgacagg aagcccgccg ccgtgctcgc gcctgccgga tacgccgcgc
     1621 ttccggtatt gttcagttcg gcaaagttag acggcagctg gctgcggtgg aattccgcca
     1681 gcggcagacg ccagaatggt tcgttctctt gtgaggcact cgccagtaaa cgaccggcaa
     1741 gcgcatcgtc aaaactgaac agcgcgtgat aatcattacc cagcgcagtt ttcgccgccc
     1801 cggtgagggt cgccgcatca atgatcattt ccggtttctg cgcactggca tcaatcagac
     1861 catcggcaag caccagacgc ccttcggcat cagtgttcat cacttcaact tttttaccgt
     1921 tgcgataggt gatgatatcg cccagcttga acgcattgcc gctaatcagg ttatccgcac
     1981 agcagaggaa cagcttcacg cgcttgttca gtccgcgcat aatggcaaat gccagcgccc
     2041 cggtaaccgt tgccgcgccg cccatgtccg acttcatcga gtccataaac gcagtctgtt
     2101 tgatgctgta gccgccggag tcaaaagtga tacctttacc taccaggcac gcgtacaccg
     2161 gtgcttcttt atcgccagtt gggttgtaat ccagcgccag caataccgga gaacgttctg
     2221 aaccgcgccc gacagtgtgc agccccatat aaccttgctc acgcagatct tcgcctttgg
     2281 tgatccgata agtcacacga tcgcccgcga cgttgctgat cagatcaaca gcacgctgtg
     2341 ccagttgcga tggtcccaat tcttctgccg gtgcgttgat ggtgtcacgc acccagtcga
     2401 tgatcatcag gcggttatcc agttcctggc gctgggcatc gtccagatcc ggccactcca
     2461 ctttacgcgt gcctttcggg cctttataac cttgccagaa tgcccagcag cgatccgcat
     2521 cccagccttc accgcttaac tgaacatgct tgatgcccag accgtcaatc ttgcgggccg
     2581 cacgctggat cagccccaga tcgtctgccc cgttcaaatg cagggtaatg ccgtcattat
     2641 taatgctgta agttgctttt tctccccagc gcgcgtcggc aggttgggta gagagggtaa
     2701 tcttcatcgc ttctgtcatt ttagttatcc ttattcgttt aacagcaaaa cgggctaccc
     2761 ccaggcagcc cgttcattca ctcggcttcg tccagccaga ctaacaaaat cgcttcgagg
     2821 attttctcgt tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca aatccactga
     2881 tgcatatcgg tgaatcgaac cgttttcgga tcaagatcgg gatacgcatc gtacagtgct
     2941 tcgccaattt cgcggctatc ggtccactta agtcccatac taacctctgt taatgctcac
     3001 gcgcatggtt gatagtgtaa cgcgggattt cgaccactaa atcttcgtcg gtgacgcgcg
     3061 cctggcagct taaacggctt tccggctcca gtccccaggc tttgtccagc atgtcgtctt
     3121 cctgctctga gctttccggc agtgagtcaa aaccttcacg aacgatgcag tggcaggtgg
     3181 tgcaagcaca ggatttttca caggcgtgtt caatctcaat accgttacgc agcgctgcgt
     3241 cgagaatggt ttcaccgcta ttagcttcca gaacagcgcc atcagggcag aggtcctgat
     3301 gaggcaaaat aacaatcttt ggcatattaa acctcgtcca cggaatggcc tttcagcgca
     3361 cgacgaaccg actggtccat gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt
     3421 ttaatcgctt gttcgatggc gtcaacatca tcgccctgcg ccacttcact caggtgagcg
     3481 gcagcattgt caatgacctg acgctctgcg gcgcttaaca gcgcggcatc agcagccagc
     3541 gcgccgtgca gactttccag cacacgcgcc gcttctactt tttgttctgc cagcatacgg
     3601 gcttttacgt cctgctcggc atagctcatt gagtctttga tcatcgaagc gatttcgcta
     3661 tcggtcagac cgtaagacgg tttgacctga atagacgcct caacgccggt ggatttctcc
     3721 atcgccgtca cgctcaaaag accgtcggca tcgacctgga acgtcacgcg aatatgcgca
     3781 ccgccagccg gtagcgccgg aataccacgc agcgcaaaac gcgccagtga gcggcagtcc
     3841 tgcaccagtt cgcgctcacc ctgcattaca tggatagaca tcgccgtctg accatcttta
     3901 aaggtggtga aatcctgagc gcgggccacc ggaatagtgg tattacgcgg aatcactttc
     3961 tccaccaggc cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc aagcagcagc
     4021 atttcgctgt ctggcttgtt acccaccaga atatccgcct gaatcgccgc gccaatagcg
     4081 acgactttat ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc gcctacccgt
     4141 tcacgcacca gcggcacgcg agtagaaccg cccaccatca ccacttccag cacttcgtca
     4201 gcttctacac ccgcgtcttt cagcgcgcga cgacaagcca gtaaggttcg ttttaccagt
     4261 ggcgcgatca gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc cgcaacgtta
     4321 acggtcacgg agtccgcatc gctcagcgcg attttggctg caatggtggc atccagcagt
     4381 tcacgctgaa cgcggttatc gctacgatca ggaatgtccg cctgctcgcg aatgtaatcc
     4441 gccagcagat ggtcgaaatc atcgccgccg agcgcggaat caccgccggt tgccagcact
     4501 tcaaacacgc cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc accgaggtca
     4561 taaacagcga tcacgccttc ctgaccggaa tccagcccgt aggcgatagc cgcagcagtc
     4621 ggttcgttaa gtaagcgcag aacatgcaac cccgccagac gcgccgcgtc tttggtgccc
     4681 tgacgctggg catcgtcaaa gtacgccgga acggtgataa ctacaccatc cagctcgcct
     4741 gccagggctt cagttgcccg cgccgccagt gctttgagga tgtccgcaga aacgcgcacc
     4801 gggttcagta gccccgccgc cgtttcaatc atcggcaggc cgttttcgct ggcctggaat
     4861 tgataaggca gatgcggata gcgttgctgg atatcagcca gcgagcgccc catcaggcgt
     4921 ttaacagaac taatggtgtt ggcggtgtca agcgctgcgt tagtacgcgc gtcataaccc
     4981 accgaatgcc cttgctgttg atagtgaaca acagatggca gcaggtgacg gccttcatgg
     5041 tcggctaacg tttcggcctg accgctgcgc actgtcgcca ccagcgagtt ggttgtgccc
     5101 aggtcaatac cagccgccag acgacgctga tgcggcgcag cactcaaacc aggttcacta
     5161 atttgtaata aggccatgtt tagcttccag aaattaaaaa tcgagcagtt tttcttcgag
     5221 ttgttcggca ctgctccgca gtttatcgag aaaacgcagc ttacgcaccg tatccgccgc
     5281 cgcgtcccac gcctcgttgt ctaactgttc aaccatcaac tgatggcggg tatcaaacat
     5341 ctttttcaca cgtttgataa agctttccag ccgcgcttca tctttcgcct gttcgatctc
     5401 gtccagctct tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc gcacagtatg
     5461 ctgctcgctg gcgagatcaa aaccgtgtaa agaaagcaaa tattccgcgc gcattaacgg
     5521 atgacgcagc gtttgccagg cctggttaat ggttgcagat tgctgtacgg cggcgagttg
     5581 ttccgccagg cttccgctgg cgaatttatc aggatgatac tgacgttgta gatcctgaaa
     5641 acgcaggctc agtgcctggg tatcgagttg ataacgggca ggcaagccaa agagggtgaa
     5701 gtaatccata acattctcag ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca
     5761 aaccccacgc gcaggcgacc acggtggggt tatcggtatg cgcatcaaac gtggaagctt
     5821 tcgccgcaac cacactcatc tttaacgttc gggttggtga atttaaaccc ttcattcagg
     5881 ccttctttta cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc
     5941 acgactttca cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat
     6001 tccagtacat aagccatacc tgaacatccg gaggttctca cgcccagacg caggccaaac
     6061 cctttgccgc ggttagccag aaaggtattt actcgcgctg ctgcactgtc gctcagtgta
     6121 atcgacataa ccaaacctca actcttattt tgcttcacgt ttgcttttat agtccgcaat
     6181 ggcggctttg atcgcgtctt ctgccagaat agaacagtga attttcaccg gcggcagttc
     6241 aagttcttca gcaatatcgg tgtttttgat cgcctgcgct tcgtcgagag acttcccttt
     6301 cacccattcg gtgaccaggg agctggaagc gatagcggaa ccgcagccgt aagttttaaa
     6361 acgcgcgtct tcaatgatac cttcatcgtt gactttaatc tgcaacttca ttacgtcgcc
     6421 acaggccggt gcacccacca tgccgctgcc gacgttctcg tcgttgttat caaaggaacc
     6481 cacgttacgc ggattctcgt aatggtcgat aactttttcg ctgtaagcca ttataaattc
     6541 tcctgattcc gataccgatt aatgatgagc ccattcgatg ctgttcagat ccacgccctg
     6601 cttgtacatt tcccacagcg gagaaaggtc acgcagacga ccgatggatt tacgaactaa
     6661 ctcgatggtg tagtcgatct cttcttcagt agtaaaacga cctaaagaga aacggataga
     6721 gctatgtgcc agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg gttcgaggct
     6781 tgctgacgta caggcggaac ctgaagaaac tgcgaggtct ttcagcgcca taatcagcga
     6841 ctcaccttca acgtagttga agctgacgtt gagaatgttc ggcgcaccgt gttccaggtc
     6901 accgttcagg taaacttctt cgatatcttt gatgccgttc cacagacggt tacgcaggcc
     6961 gcgcagacgt tccatctcgg tcgccatctc ttcttttgcg atgcgatagg cttcgcccat
     7021 tccgacgatc tggtgaacag gcagagtgcc ggaacgcata ccgcgctcgt gaccgccgcc
     7081 gtgcatttgc gcttcgatgc gtacacgcgg tttacgacgc acatacagcg caccgatacc
     7141 tttcgggcca tagattttgt gaccggagaa agacatcagg tcaactttca actggctcag
     7201 gtcgattggc agtttgccca cgctctgggt tgcatcaacg tgatagataa tgccacgagc
     7261 acggcacatt tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt tatttacgtg
     7321 catgatggaa acaaggatgg tgtcgtcacg catcgccgct tcgagttctt tcaggtcgat
     7381 aataccgtta cgctgcggtg ccaggtaggt gacttcgaaa ccttcgcgct ccagctgacg
     7441 gcaagtatcc agtaccgctt tgtgttcggt tttgctggtg atgatgtgct tgcctttttt
     7501 ctgataaaag ttggctgcac ctttgatcgc caggttgtca gattcggttg caccagaggt
     7561 aaagacgatt tcacgcggat cagcgccgac cagatcggca atctgattac gggcgatatc
     7621 taccgcttct tcagcctgcc agccgaaacg gtgagaacga gaggccgggt taccaaaggt
     7681 tccgtccatc gtcatgaact gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt
     7741 tgcggagtag tcgagataaa tcggtaattt cattgctcta taaactccgt acatcactca
     7801 atgcaaggaa tcaggctacc ggctggatgt acgaccgtgt ttacggagta tttagcactc
     7861 cggcctgatt ctgaattctt tttattaagc gcgtaactta acgtcgatcg cgtcttgtgt
     7921 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
     7981 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
     8041 acgccacagc gcgtgggtca ggcatttatc gccgccctgg cagccgcctt taccctgaca
     8101 acgggtggca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
     8161 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
     8221 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
     8281 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
     8341 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
     8401 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
     8461 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
     8521 cattttttta tcgtggcgtt tgtacctgtt gtcggatgcg gtgtgaacac cttatccgac
     8581 ctacgattct gtacattgta ggcctgataa gacgcgttaa gcgtcgcatc aggcaaccac
     8641 tgttattcgg ccttgttacc tttattctgc tgctcaatag aagccagaat cccgcgcagg
     8701 atattcaatt cctggctttc cgggcgcgca cgggtaaaca gacggcgcaa tttattcatc
     8761 acctgccccg gatggttttc acggataaaa ccggttgcca gcagcgtttg ttccagatga
     8821 ccataaaaac gctccagatc atcgaccagc ggatacggcg tctcttcatg ctcgacctgc
     8881 tcgccatttt cctgagttgc cagccaggcc atacgcactt cataggcaat aacctgaacc
     8941 gccattgcca ggttcagcga actgtattcc gggtttgccg caatcgcgac atgataatgg
     9001 catttctgca actcttcatt ggtcaagccg acgcgctcac gaccaaacac cagcgccacc
     9061 ggggtatttg ccgcttcagc gacgcttttc agaccgcatt cgcgcgggtc gagcatcggc
     9121 cacggcagcg tgcgggagcg tgcgctggtg cccaccacca ggctacaacc ggctaacgct
     9181 tcgtcgagcg tatcgacgat gtgagcatta ccaatcacat cgctggcccc tgcagccagg
     9241 gcaatcgcct gggagtcggg tttcaccagt ggattaacca gccacagatt ggttaatccc
     9301 attgttttca tggcacgggc aacagaaccc atattgccgg tgtgtgacgt ctccaccagc
     9361 acaattcgaa tattttgcag cattgtcttt cttcatctaa agattattca cgcatcttat
     9421 cataaaacga agacagatgc cgatctcgct gctatactct gcgccgtttt cccgttcttt
     9481 aacatccagt gagagagacc gatgcatccg atgctgaaca tcgccgtgcg cgcagcgcgc
     9541 aaggcgggta atttaattgc caaaaactat gaaaccccgg acgctgtaga agcgagccag
     9601 aaaggcagta acgatttcgt gaccaacgta gataaagctg ccgaagcggt gattatcgac
     9661 acgattcgta aatcttaccc acagcacacc atcatcaccg aagaaagcgg tgaacttgaa
     9721 ggtactgatc aggatgttca atgggttatc gatccactgg atggtactac caactttatc
     9781 aaacgtctgc cgcacttcgc ggtatctatc gccgttcgta tcaaaggccg caccgaagtt
     9841 gctgtggtat acgatcctat gcgtaacgaa ctgttcaccg ccactcgcgg tcaaggcgca
     9901 cagctgaacg gctaccgtct gcgcggtagc accgctcgcg atctcgacgg taccattctg
     9961 gcgaccggct tcccgttcaa agcaaaacag tacgccacta cctacatcaa catcgtcggc
    10021 aagctgttca acgaatgtgc agacttccgt cgtaccggtt ctgcggcgct ggatctggct
    10081 tacgtcgctg cgggtcgtgt tgacggtttc tttgaaatcg gtctgcgtcc gtgggatttc
    10141 gcggcaggcg agctgctggt tcgtgaagcg ggcggcatcg tcagcgactt caccggtggt
    10201 cataactata tgctgaccgg taacatcgtt gctggtaacc cgcgtgttgt gaaagccata
    10261 ctggcgaaca tgcgtgacga gttaagcgac gctctgaagc attaatgact caggcgggtg
    10321 atatcactca cccgccctcg cctttcaggc gctattccga aatacttcct caccgcttta
    10381 ctttctttcg tcactctccc accatctttc cccgattaat ggatgataaa gaagtcgcaa
    10441 ggaaaggtta tggcactgcc agtgaacaaa cgcgttccca aaattctgtt tattctcttt
    10501 gttgttgcct tctgcgttta tttagtgccg cgcgttgcca tcaacttctt ctattatcct
    10561 gacgataaaa tttacggtcc cgacccctgg tcggcggaat ccgtcgaatt tacggctaag
    10621 gacggtactc gtctgcaagg ctggtttatt ccttcttcga cgggccctgc tgacaacgcc
    10681 atcgcaacca tcattcatgc tcacggcaat gccggaaata tgtccgccca ctggccgctg
    10741 gtcagttggt tacctgagcg taatttcaac gtttttatgt ttgattatcg cgggtttggt
    10801 aaatcaaaag gcactccgtc ccaggccgga ttgctggacg atacgcaaag cgccatcaat
    10861 gtggtgcgcc atcgcagtga tgtaaaccca caacagctgg tgctgttcgg gcagagtatt
    10921 ggcggggcga atattcttgc tgttattggt cagggtgatc gtgaaggcat acgtgcggtg
    10981 atcctcgact ccacatttgc ctcttatgca accatcgcca accagatgat ccccggcagt
    11041 ggctacttac ttgatgagag ttacagcggc gaaaattaca tcgccagcgt cagcccgatc
    11101 ccgcttttac tcattcacgg taaagccgat cacgttatcc catggcagca cagcgaaaag
    11161 ttgtatagcc tggcaaaaga gccaaaacgg ttgatcctaa tcccggatgg cgaacacatt
    11221 gatgcttttt ccgatcgtca cggcaatgtt tatcgcgaac agatggtgga ctttatcctt
    11281 agcgcgttga atccgcagaa ctaacccatg atcgctagca cgataatcat tcacaaaacc
    11341 accttaagac atgctaatcc actggtcaga acagtttaaa atgagaaaaa ttctgtgacg
    11401 cttgccaaca tttctgatga ttagcattcc cttcgccatt tccctgagca aactttagct
    11461 attcttatca attatgctta tgggagatct acagatgatg cctacgcttg ctccaccatc
    11521 tgtcctttcg gctccccagc gccgctgcca gatcttgctg acgctctttc agccggggtt
    11581 gaccgccacc atggcaacct tcagcgagct taatggtgtg gatgatgata ttgccagtct
    11641 tgatatcagc gaaacaggac gggagatcct gcgctatcat caactcacac tgacgactgg
    11701 ttatgacggt agctaccggg ttgaaggtac agtgcttaac caacgtttgt gtttatttca
    11761 ctggctacga cgtggtttcc gtctgtgtcc gtcatttatt accagccaat tcacccccgc
    11821 cctgaagagt gaactgaagc ggcgcggaat tgcgcgtaac ttttacgacg ataccaatct
    11881 acaagcgtta gtgaatctct gctcccgacg gctgcaaaaa cgctttgaat cgcgcgatat
    11941 tcatttcctg tgtctgtatc tgcaatattg tttgctgcag caccacgctg gaattacgcc
    12001 tcagtttaat ccgctccaac gtcgctgggc ggaatcctgc cttgaatttc aggtagcgca
    12061 ggaaattgga cgccactggc agcgtcgggc gctccagcat gtaccgcctg atgagccact
    12121 gtttatggca ttactttttt ccatgttgcg ggttcccgat ccactgcggg atgcgcatca
    12181 gcgggacaga caattgcgtc agtctatcaa acgtctggta aaccattttc gtgagctggg
    12241 aaatgttcgt ttttatgatg aacaggggtt atgtgatcag ctttatatcc acctcgccca
    12301 ggcgttaaat cgcagtttgt ttgccatcgg tattgataat accctgccgg aagagttcgc
    12361 cagactgtat ccacgcctgg tacgcaccac ccgcgcggcg ctggccggat ttgaaagtga
    12421 atacggcgtc catctttctg atgaggaaag tggtctggtc gcggtgattt tcggtgcctg
    12481 gctaatgcag gaaaacgatc tgcatgaaaa acagattatt ctactgaccg ggaatgatag
    12541 cgagcgagaa gcgcagattg agcagcagtt acgcgaacta acgttactgc cgctcaacat
    12601 taagcatatg tcggtaaagg catttttgca gacaggcgct ccgcgtggcg cggcactgat
    12661 tattgcgcct tataccatgc cgttaccgct cttttcacca ccgctgatct atacggacct
    12721 gacgctgaca acacatcaac aggagcagat ccgcaaaatg ctcgaatcag catgagggaa
    12781 caactttcgg gcgcaaaaac attgccggaa gcgccaccag cgccattacc cagaacacgc
    12841 cgtggcccag atattgatac aggaaaccgg cgaaaacggt catgatagcg atactgccgc
    12901 ccatcgcgac ggcagagtaa accgcctgta aacggatgac ttcgctaacc tggcgagcag
    12961 caatataacg catggcggcc aggtggcaga ccgtgaaggt gccgcaatgc agaatttgca
    13021 ccactatcaa ccacggcaac gccgtagttg ctcccataat gccccagcgc actacgccgc
    13081 aaatcgccga gatcaacagc atatcgcgag cactacaacg gcggaaaagt ttattactca
    13141 gcgcaaagat aatgacttcc gccaccacgc ccagcgacca caaatacccc accgccgagg
    13201 ccgagtagcc agctgcctgc cagtaaatgg cgctaaaacc gtaataggcc gcatgtgccc
    13261 cctgcaataa acaaacgcag gccagaaagc gccagttctg gcgaaccagc gccaaccacg
    13321 cagaccaacc ggtgctctcc tgctggcggc ttgccccttg tggctgaatc gtcggacgga
    13381 tgagaaagcc gagcagcatg gatgccacgc ccaacgtcaa cagcacgagg atcacccgat
    13441 aatcaaacat agtgaccagt ttgcccgtca gcgccgagcc aatgacaaac gccaccgagc
    13501 cccacagtcg cactttgccg taatcaagcg ggaactgctt ttgccacgta ttcgccagtg
    13561 catcggtcaa cggtaccagc ggtgagaaaa agaggttaaa gccaatcatc accagcatca
    13621 gccacgctac gtgcgccccc gcccagaagg cgacagcaaa gagaagtggc agcagtgcca
    13681 gcacgcgcaa ggcggaaatc aggcgggaag gatcgctgac gcggggcgcg atgagcaaac
    13741 tcccgagaaa acgggcaacc agacctgccc ccaataacag gccgatggtt tctggcgtta
    13801 aaccaatccc tttaagccag acgctccaga aaggtaaaaa aatgccgtaa ctaaaaaagt
    13861 atgtgaaata gccgagcgcc aaccagcgcg tggattgcaa aaccatgagt ccctcccgtc
    13921 aggaggcgtt agtctggggt taatcgaatg ttggtgcaag ttgaaaaata agaatattcg
    13981 agcctgcttc ccgatgtctg taacgccagt tcacaagcac taaaaaactg cgatccgcga
    14041 cgcatgttgc aaaaataatg cgtaattatg ccgttacgct tgccaaacgt tcctgcacaa
    14101 tggcgatgaa atcgcgtaat gccagcttca tctccccttt cttccacgcc atcagtaaag
    14161 caatggaagg cacatttccg gcaatgggac gaaaaacaac ctgcccggta ttaaaattat
    14221 tcatataacc gggtatcaaa gtgacgccca gccccatgcc caccagattc atggtcacca
    14281 gaatattcgt tgccacctgg acgatatttg gctggctgtt ttcttgcgca aaccacgctt
    14341 taacgatcgg cgcaagcgaa ccggaatacg ccggatcggt actgacgaaa ttcacgccat
    14401 ccagttgcgc ggcggtgatc tctttttcat gtgctaaagg gtgatcaacc ggtaacacca
    14461 ccactaatgg ttcgtcaaaa agctccagat aatcaatatc cgggctataa acaggatggc
    14521 gcattaagcc gacgtcgagt tcaccacggc gaattttttc ctcctgttgc gtggtgatta
    14581 aactcaccag ctcaatcaag gtgtctggct gtctgagacg aaacatcggt aatacttttg
    14641 gcaataaatt cacttccgcc gatggcacaa agccaatggt taattgtctg tcttcctgaa
    14701 caattttccg cgcccgtaat ttggcatttt ccgcttgttc gaggattgcc agcgcgtcct
    14761 ggagaaaaca ttctcccgcc gccgtcaacg cgactttgcg cttatccctc accagtaacg
    14821 gaacaccgac acagttttca agatcgcgga tctggctgct taacgaaggc tgtgaggtat
    14881 gcagtttttc cgccgcacgg gtaaagttca gtgcctgcgc cactgcgacg aaatagcgta
    14941 aatgccgtag ttccatcacc ttccccttgt tatcgaaaaa acgtctcagc tggtagaaat
    15001 taactatttc acatattagc aaccaaccag caacatcctt atggcacaaa aatagaaggt
    15061 caatacatct tatctttcag gattaaaaaa tatgaccaca ccctcagatt tgaacattta
    15121 ccaactgatt gacacccaaa acggtcgggt cactccgcgt atttataccg acccggacat
    15181 ttaccaactg gagcttgagc gtattttcgg tcgttgctgg ctatttctcg cccacgaaag
    15241 ccagatccca aaacccggtg atttctttaa cacctacatg ggagaagatg cagttgtcgt
    15301 ggtgcgccag aaagacggca gcatcaaggc gtttctcaac caatgccgcc accgggccat
    15361 gcgtgtgagt tatgcagatt gtggcaacag tcgcgccttt acctgcccgt atcacggctg
    15421 gtcttatggc attaacggcg agttgatcga tgtaccgctg gaacctcgcg cctacccaca
    15481 agggttgtgt aaatcccact gggggctaaa cgaagttcct tgtgtggaga gttataaagg
    15541 actgattttt ggcaactggg ataccagcgc accgggcctg catgattacc tgggtgacat
    15601 tgcctggtat ctggatggca tgctggatcg tcgcgaaggc ggcaccgaaa ttgtcggcgg
    15661 cgtacaaaag tgggtgatca actgtaactg gaaattcccg gcagagcagt tcgccagtga
    15721 ccagtatcat gctctgttca gccatgcttc tgccgttcag gtattagggg cgaaagatga
    15781 tggcagcgat aagcgcctcg gtgatggaca aaccgcccgc ccggtgtggg aaaccgccaa
    15841 agatgcgctg caatttggtc aggacggtca cggtagcggt ttcttcttta ctgaaaaacc
    15901 ggatgctaat gtctgggtcg atggcgcagt ttcaagctat taccgcgaaa cctatgccga
    15961 agcagaacaa cgtttaggtg aagttcgcgc cctgcgcctg gcgggtcata acaatatatt
    16021 ccccacgctt tcatggctaa acggcactgc cacactccgc gtctggcatc cgcgcggccc
    16081 tgatcaagtt gaagtgtggg cgttctgtat tactgacaaa gccgcctccg atgaagttaa
    16141 agccgctttt gaaaacagcg ccactcgtgc ttttggtcct gctggttttc tcgagcagga
    16201 tgactcggag aactggtgtg aaatccagaa attgcttaaa ggccaccgcg cccgcaacag
    16261 caaactgtgt ctggaaatgg ggcttggtca ggaaaagcgc cgcgacgacg gcattcctgg
    16321 cattactaac tatatctttt cagaaactgc cgctcgtgga atgtaccaac gctgggccga
    16381 tcttctgagt agcgaaagct ggcaggaagt gctcgataaa accgccgctt accagcagga
    16441 ggtgatgaaa tgagtgcgca agtttcactg gagttacatc accgcattag ccagtttctc
    16501 tttcacgaag ccagtctgct ggatgactgg aaatttcgtg actggttggc acagctcgac
    16561 gaagagattc gttacaccat gcgcaccacg gttaacgcgc aaacacgcga ccgtcgcaaa
    16621 ggcgtccagc cgccgacgac ctggattttc aatgacacca aagaccagct ggagcggcga
    16681 atcgcccgtc tggaaacggg catggcctgg gcagaagagc cgccgtcacg cactcgtcac
    16741 ttaatcagca actgccaggt aagcgaaacc gacatcccaa acgtatttgc cgtgagggtg
    16801 aattatctac tttatcgggc acaaaaagag cgcgatgaaa cattctatgt tggaacgcgt
    16861 ttcgacaaag ttcgccgtct ggaagatgac aactggcgct tgctggaacg ggatatcgtc
    16921 ctggatcaag cggtgatcac ttcccataac ctgagtgtac tgttctgatg aatcgaattt
    16981 atgcgtgtcc cgttgcggat gtgccggagg gtgaggctct ccggatagat acctcgcccg
    17041 tcatcgcctt gttcaacgtg ggcggcgagt tttatgccat taacgatcgt tgcagccatg
    17101 gtaatgcgtc gatgtcagaa gggtatctgg aagatgacgc cacggtggag tgcccgctac
    17161 acgccgccag tttttgcctg aaaacgggga aagcgttatg cctgcccgcc accgatccgc
    17221 tcaccactta tccagtacac gttgaaggtg gtgacatttt catcgactta ccggaggcgc
    17281 agccatgagc gatctgcata acgagtccat ttttattacc ggcggcggat cgggattagg
    17341 gctggcgctg gtcgagcgat ttatcgaaga aggcgcgcaa gttgccacgc tggaactgtc
    17401 ggcggcaaaa gtcgccagtc tgcgtcagcg atttggcgaa catattctgg cggtggaagg
    17461 taacgtgacc tgttatgccg attatcaacg cgcggtcgat cagatcctga ctcgttccgg
    17521 caagctggat tgttttatcg gcaatgcagg catctgggat cacaatgcct cactggttaa
    17581 tactcccgca gagacgctcg aaaccggctt ccacgagctg tttaacgtca acgtactcgg
    17641 ttacctgctg ggcgcaaaag cctgcgctcc ggcgttaatc gccagtgaag gcagcatgat
    17701 tttcacactg tcaaatgccg cctggtatcc tggcggcggt ggcccgctgt acaccaccag
    17761 taaacatgcc gcaaccggac ttattcgcca actggcttat gaactggcac cgaaagtgcg
    17821 ggtgaatggc gtcggcccgt gtggtatggc cagcgacctg cgcggcccac aggcgctcgg
    17881 gcaaagtgaa acctcgataa tgcagtctct gacgccggag aaaattgccg ccattttacc
    17941 gctgcaattt ttcccgcaac cggcggattt tacggggccg tatgtgatgt tggcatcgcg
    18001 gcgcaataat cgcgcattaa gcggtgtgat gatcaacgct gatgcgggtt tagcgattcg
    18061 cggcattcgc cacgtagcgg ctgggctgga tctttaagga agcacgatga aagaaaaaac
    18121 gatcattatt gtcggtggcg ggcaagcggc ggcaatggct gcggcctcgc tacgccagca
    18181 agggttcacc ggtgagctgc atctgttttc cgatgagcga catcttcctt atgaacgacc
    18241 tccactctcg aaatccatgt tgctggaaga ttccccgcag ttacagcagg tgttacccgc
    18301 taactggtgg caggaaaaca atgttcatct gcattccggt gtaaccatca aaacgctggg
    18361 tcgcgacaca cgagagttag tgttaaccaa cggcgaaagc tggcactggg atcagctttt
    18421 tatagcaacc ggcgcggcag ctcgaccgct gccgttgctt gatgcactgg gagaacgctg
    18481 ctttacccta caccatgccg gtgatgccgc cagactgcga gaagttctgc agcccgaacg
    18541 gtcagtcgtg attgtcggtg ccggaactat tggtctggaa ctggctgcca gcgccacgca
    18601 gcgcagcgca gcgcagcgca gatgtaaggt gacagtgatt gaactggcgg caaccgtcat
    18661 gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc agcaggctgg
    18721 tgtgcgcatt ctgctcaata atgccattga acatgtggtc gatggtgaaa aagtagaact
    18781 gacgctgcaa agtggggaaa cgcttcaggc tgatgtggtg atttacggta ttggtatcag
    18841 cgccaacgag caactggctc gcgaggccaa ccttgatact gccaatggca ttgtcattga
    18901 tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg caatcactcg
    18961 tcttgataat ggtgcactac accgctgcga aagctgggaa aacgccaata accaggcgca
    19021 aattgccgct gccgcaatgt tagggctacc gctaccgcta ctgccgccgc cgtggttctg
    19081 gagcgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct
    19141 ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt aatctgcaaa acggcgtgct
    19201 tatcggtgcg gtcacgctga atcagggccg tgagattcgc ccaattcgca aatggatcca
    19261 gagcggcaaa acgtttgatg cgaaactgct gatagatgag aacatcgcgc ttaaatcact
    19321 gtaatcagga taattagcga atatctcaat gcctggggcg tggcgaggtg caagagtgtg
    19381 tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaacacac tacgttattt
    19441 tgattttgga gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcttaat
    19501 tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc
    19561 ctcgttgggc gcgccaatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc
    19621 cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc tgtttatttt
    19681 ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgcggt
    19741 tgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcgt tcttgctatt
    19801 ggcaattacc ggaccggggg caatttctct cgatcggcgt taggaaagat gccggatgcg
    19861 gcgtgaacgc cttatccggc attttataaa ttacagccac tctacccgca acgacatctc
    19921 tgaggttgtt gattcccctg gtgctagcgc aatgagatca cccccttccg ggcgatgatg
    19981 ctcatccggc gcatggctca tcggttccag acagaagaaa tcaaacgcat atcctttatc
    20041 aaacgcaggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttccatgat
    20101 gatggcataa ccttcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc
    20161 attgttcacc cactgacgcg gcaacggcgc gagctggtta aaatccagtt cctgcggtag
    20221 ctgctcgcaa aactcacccg ccagccactg ctcccgttcc agccagtaac cgctcgcctg
    20281 cgcctgaatc cgcgtttgtg gtgacaacgg aaaataagga tgccagccgg taccaaatgg
    20341 cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa
    20401 atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca
    20461 caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag
    20521 atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa
    20581 acggttgccg ctcacccggt ttgcaaatgg caccagcgga aaacatgagg catcggttgc
    20641 cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcgcgccaga agccttcgat
    20701 tacgccgccc tggtcagaaa cgtccagctt tagcgacccg tgggataagg tatagatggt
    20761 catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc
    20821 atcagcacat aggcatcacc tgcctgttct agcgagaagc gatgggtaat ggcgttacgc
    20881 ggccacagct tccagtcagt caaatcgtgg gcgcattttt ccatatggaa cagactggtc
    20941 acccaggagc cgataatccg ccgttgatgg tgcatcagat cagcgctgac ctcgaattcc
    21001 acttttccgg tttcaccaat gtaaaccacc cgcccccagt cagcagtgga ttgcagtgcc
    21061 agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc accgtgggta
    21121 agttcagcaa ttatctgcgg cagaccttcg gtggttgcta aatagccgtg atccatcccc
    21181 cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat gatccgtttt
    21241 gcaccgcgac ctttcgccag catcatcgcc atcatgccga ccggccccag accgaccacc
    21301 agcacgttat cgctgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg
    21361 ccgcaactga taaacgcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct
    21421 ttttcttccg ccagcagata ttcggcatga ccgccgtcac gctgccagcc gtaagccgct
    21481 tttcctttgc cagtacaaga gataggaaag ccgcggcggc agttcgggca aaaaccacag
    21541 ccagaaatgt gatacaccag cacgcggtcg ccctctttaa aatggcggca gccttgcccc
    21601 atcgccacaa tctgcccgca cggttcatga ccgttgataa atccctggta taacggttta
    21661 tcgggtgccg ccgccgtggc acggtgttga tgatagatat agtggacatc gcttccgcaa
    21721 atcccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg caccgcaact
    21781 tcccgcagat cgacggttga atttcctagt aaataagcag ccagcatcgt tttcataaat
    21841 ccctcattaa caatacgatt aattttcatc cctgcagcac ccgcgccagg ggcgttaagg
    21901 tttagcgttt cgctttactg cttcgctggg tcagcaagat attcgccagc accgccacca
    21961 cgatgatgac gccgcgtacc acctgctgga aaaaggagtt aataccgagc agcaccagac
    22021 cgttaccgat tagcgtaatc accagcacac caagcaatgt accgaacagg gaaccgcgac
    22081 cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt
    22141 ttgcggcacc tgcgttaccg gaaccgaggc gtgccgccaa caaaatgccg gtcaccgccg
    22201 ctaataatcc cgaaagggta aagataagaa tgcgcactcg acgaacgtta ataccgcaca
    22261 actgcgccgc cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggtttttc
    22321 ggctaatgaa cacaaacagc gcaaacaaca ccatcatgat cagcgcggat actggcacac
    22381 cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcagtg
    22441 cgttggtcat aaacagcccc attccgcgca gagcgctcca cagccccagt gtggcaacga
    22501 aacttggtac gttaaacacg ccgcgcagca ccccggcgag cgttcccatc agcgcgccta
    22561 acagcaacac cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca
    22621 ggcacaccga gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa
    22681 taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca
    22741 tctggttatt caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg
    22801 caatcaccac cagcaaaccg atctcattaa tatggcgact gacaaattgt ttgagcgaga
    22861 cgctcttgcc ctgcggcaat ggtaatgacg aagcatacat cttcaggttc ctcggtaaaa
    22921 ttcatcagtg cacagacaga atggcggaca tcagctcatc cacattgacc ggcgagtgaa
    22981 actcctgcga gaacgtgccg tgctgtaata acaggatgcg gtcacacacc agcggcaatt
    23041 cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc tcacggacaa
    23101 tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca
    23161 aaatctggct ggcggcatag acccaacgac cgatcaccac tttttgctga ttgccaccag
    23221 aaagcgtgcc gatgggtgtt tcgctactgg cagccttgac cgtcatccgc tgcatcacct
    23281 cttcggtcag gcggcggatg gtggaccatt gcagcacacc gttggtgctg attttttgcc
    23341 gattggtcag cactgtattt tcgtcaacgc ctagccaggg aatgatcccc gcttctttgc
    23401 ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcatga
    23461 ttttctcgcc gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct
    23521 tcagcaattc actgcgccct gcccccagca ggccagcaat gccaagcact tcgccacgac
    23581 gtagcgtaaa gctgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagta
    23641 cggcctgatc cacaatttcc tgaggggcaa ccggcgcaat atcaacgtga tcacgcccta
    23701 gcattagcga cacaatatga tgcgtggagg tgttttcgag catcacatcg cccgccacct
    23761 gaccatcgcg cataacggtg gcataggagg caatgcggcg gatttcttcc atccggtggc
    23821 tgacataaat caccgccacg cccagtgccg acatcttttt caccgcactg atcaccagtt
    23881 caacttctgc actggcaagc gagctggtgg gttcatcaag aatgaccacg cgcggctcgc
    23941 ccttcatcac ccgtgcaatt tccaccagtt gcttttgcgc cgggcttagc gttgaaacaa
    24001 gttgttcagg actaacgtca acgcccagcg cctgtaagca acgttgggca tcctgcgcca
    24061 tttgcaggta atcaatcatg ccgttgcggc gaggccattg accaaggcag aggttttctg
    24121 ccaccgtcag cccttccacc agacttaatt cctgataaac cgcacgaacc cccagttcag
    24181 cggcacggcg agtcaacgta gcttcgtcac cttccagtcg cgtctcgcca atccagatat
    24241 caccgctatc cgggcgttcg ctgccggtaa gcattcgaat gagagtcgat ttgcccgcgc
    24301 cgtttttacc taacagcgca cgaacttcgc ctttattgag cgtgaagtta acgttatcca
    24361 gcgcaacgac gccggggtaa cgcttatttc ctgccaccac ttttgctacc gggactgcct
    24421 ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc
    24481 gttgccagcc actgtttacc atcttccgtt ttggtataga gatcgattgg cacctgaatc
    24541 actttttcac cgtcggcttg tttattgata accttcaatg tttgcgcgaa aacagcattg
    24601 cccattttct taccggaaat atccactacc gctttcagca cctgattgtt ttccagctcc
    24661 tgagcaattt cggttgtcat atccgaaccg aaaacagcaa tttttccggc ctgattttga
    24721 ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg actcccccat aatggcgttg
    24781 agatccggcg tggagataat cagtttttca ccaacggaaa tcgctttatc taaaacagtc
    24841 ccttcctggt tagcgacaat ttgcgcgccg ggaacgcggg attttaatac ttcttcaaat
    24901 cctttacgtc gctgcacaca aacttcaaag gcttcgcaat tgatgacggc aattttcggc
    24961 tggtcaattt tattggcaat aaaataatcg gcggcagcgt tacccagttt ttaccaaatt
    25021 ccagtggatc gccgaccaga tacgccgaga catatttatc gacacccttt tgattaatac
    25081 aggtgttgta gcaaatcacc ggaatacccg cttcactggc gcgacgaacg gtacggctac
    25141 tgccattttc agacactgcc gataaaataa tggcatcgac attacgcgcc acgagggtat
    25201 caacaaaggt actttctttc gaaatatccc cctgggcgtt agtttcaatt aactgtacct
    25261 gtactgaaga atcttttgcc gcatcctgaa cgccctgacg cactccagcg taatatccct
    25321 gggtatcaag gtatattgca ccaatggtca tttctttatc cgcagccctg gcaaatagtg
    25381 cgctacctaa tagcgtagcc attaataata gattacgggt tgttctcatt tttttaggca
    25441 tagtgcctcc tgtagggttt ttattaacaa cggcttattc taattatttt gtgatgagcg
    25501 gcagcgcgtg ccgctcaaat attacagtta atagggttta gtgaacgtag ttaaaaataa
    25561 aaggcatcac agtggtgaat aatgccgctt tcggccaggc cggattaatc gccgtcaatt
    25621 cagcgcgagc agactcatat tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca
    25681 ccatcaggca tttttcttta tgctgctgtt gtaaatcgcc atacagcgat aacaggtcag
    25741 gttgtgaaac agcaaagaaa tccgcctcaa tactggtttt cgccatctct tgcgcccact
    25801 gtttcatttc actaaacagt tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca
    25861 ttccttgcca gaagagataa tcaaccggct gatcgttgta ataactgtgg atgttaatgg
    25921 tgcgatcgcc ggtcgccgcc agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg
    25981 cgttggcgca tatcgcctgc cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc
    26041 cttcgcttaa attctccgga taatacagcg cggcatgaag cagttcgctg gcctgctgcg
    26101 gctctctggc atcaagatgc tgccaggcgc gtaataactg gttgaggata aactgactgg
    26161 tgaccttccc ttccccgcct tcccacgggt ggaatttacg cgtggcgaga atgtccgccg
    26221 ctttgtctgc ctgacccgtg agatgccaca aattgagcag ttctgcggtc atgtcgtcgc
    26281 gtttcagcgc aatttccaga ttattttcca gacgcgccag tcgtttctcc ggtgtggctc
    26341 cacttaactt atccagcaaa tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct
    26401 gataagcatt atcaagataa cgcgcggcca gctcataatc gtgtttctta ttccacgcat
    26461 ggatcgctaa cccgcgccag ccgtcggcaa actccggcga catctctacg caacgttgcc
    26521 ataaggcaat ggctttgttg tagctacgtt tgttgtagta gaagcaggcc agtaaatggc
    26581 gagcaaacca acactcttca atactctcca gcgccgccac ttcttccagc gtattcggga
    26641 aacggacaaa ctgcgggaag acatcaatgg cttttgcgac cagttcgcca cgttcggctt
    26701 tcggcagcaa gctggcttgc aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca
    26761 gcatctctgc cgccagggtg ggcattcccc agttaatcag ttgcccggcg gtcatcagag
    26821 cgttaacgtc gcgtccctga cacagaccgc gccactgcgc gagggctgat tcgctacgac
    26881 catcgaacca gttcagccac cacagagtgg cgttcagcgg ataatcacgc agcagtttct
    26941 cgcgctgcaa acgcgcgttg tcctgacgac cacttaacac cagcagcaga ttatgcaagc
    27001 aaagcacttc ctgattggtt gggcaggcac gaagactttg ttggcaaaaa tccagacccg
    27061 cgtcgaagtt atcattacgc gccgccagtc gtgccagacc ataatagccg cctgctttac
    27121 tattgccgct ccagaccgca cgccagaaat cctcttcggc ttgttgatat tgtccctgac
    27181 gttcgtaagc actggcgcga atcaaacttg cttgtccaca ctgcggtttt ttgttcagcg
    27241 catgtgcacg tttcagcgcc tgactggcat acgccaccgc ttgcgggaaa tctgcgcggt
    27301 tatattccag catcgccagc gccaggttac agcgataatc cagcgggtcc agcgccacgc
    27361 cgcgcaggta gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc agatgctgac
    27421 cgataaacca ggcttcatct gtactggtaa tgtcttgtgc tgccagtggc gctttggcga
    27481 cgtccggcaa cggcaactcc tgcggctgat gttcctgata actcagtaca atattgccgt
    27541 cggcatcgga gagttcaatg gtcagtctgt caggattgat accgtgcaac acgccctgga
    27601 tggcggtcgc tggtgtcagg gccacggcat catcgagcaa cgcgttgcat ttgccgattt
    27661 cgcggatcgc cagacggtat ccgttcaacg gagagatggc atacagcccc cactcaatcc
    27721 cccgctcact acgctggagt tttatcaccg catcgcggga ggcattttgc accatgccca
    27781 gagaatgata aggcaggaaa tactgctcga aacgcttctc ttcgtaagca tcaagccagg
    27841 taaaatcagg ctggttatcg gcaaaaatac cggtcatcag ttcgatatac gggccgttat
    27901 tgtcggtcag actcttatcc cacgcctggc caaattcact gtgtccccaa ctccactgtt
    27961 ttttacctgg cgcaatatgg tggttggcaa cgtgcagcaa accgccatct tcatcgtgac
    28021 accacgcgcc gacaaaatcg tactgcgatt tttcggccat atatgaggtt ggaacaggca
    28081 catttttata gcgagaaatg tccactccgg cggagtagtc cactttgtag taagtgccgg
    28141 tggcgatggg gaaagcggag acggcccgtt tgccgtgatc aaacaccgca gtcacatccg
    28201 gtgggaaaac gctctgatgc ccttcacccc ctttcactgc cgggttgacc caccacaaga
    28261 aatgacgcgg cgtggcgttg ccgttataga cgcggctggc gatttccagt gccgcccggt
    28321 cagggcgcag ggtgaaacct gtcatcacct gtaaaccatg catcggctcc gtttcgccga
    28381 cccacaccgt ctgtgcaccg tcttcatggg cttcgagggt gaaatcaacg ggcataaagg
    28441 tggtcgggcg atggtgttgc ggccagttaa actcaatccc gccggagatc cacggtccca
    28501 gcatccccac cagcgcaggt ttaatgactt cattgtgata gacaaaatcg cgctgtttca
    28561 ctttatccca tgcgcgatgc acccgaccgc ccagttccgg caggatcatc actttgatgt
    28621 agtcgttttc cagccacacc gcctgctagg atttcagggt tttctgctcg ctcagcgtat
    28681 cggtcacgcc gtagggataa accgcgccgg acgatccctg ataaacgcga ttttccagga
    28741 acatgggatg tatatcctgc ggcccggttt cataggtcgg gatctcaacg cgctcttgcc
    28801 acacttttac tggagtcatg gtgccctcaa tattaacaag acatactgaa ttaaaagatt
    28861 tgtggcagtg tattgaacaa tctggcaatg ttttcgcgga ataatcacgc aattaactaa
    28921 acaaggttta gtgaagatga gagcctgcat taacaatcaa cagattcgcc accataacaa
    28981 atgcgtgatt ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg
    29041 gctggcgcaa atttcgattc cggcagtcag taatattttg caggaactgg aaagcgaaaa
    29101 acgggtggtg aatattgacg atgaaagcca gacgcgcggg catagtagcg gtacatggct
    29161 gattgcgccg gaaggtgact ggacgctgtg cctgaacgtg acgcccacca gtattgagtg
    29221 tcaggttgct aatgcttgtt taagtccgaa aggcgaattt gagtatttac agattgatgt
    29281 accgacgccg caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt
    29341 gtggccggac cgcaccatca atctggcgct ggcaatccac gggcaggttg atccggtgac
    29401 cggcgtatcg caaaccatgc cgcaagcgcc gtggacaacg ccagttgagg tgaagtatct
    29461 gctggaagag aagctgggca ttcgggtgct ggtcgataat gactgcgtga tgctggcgct
    29521 ggcggagaaa tggcaaaata attcgcagga acgggatttc tgcgtgatca acgttgatta
    29581 cggcattggc tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt tgtatggtag
    29641 cggacagatt ggtcacacca tcgttaatcc ggatggagtc gtctgcgact gtggacgtta
    29701 cggcagcctg gaaaccgtcg cctcgttaag tgcattaaaa aaacaggcgc gggtatggct
    29761 aaaatcacaa ccggttaata ctcagcttga gcctgaaaaa ctgacaaccg cgcagttaat
    29821 cgctgcctgg cagagtggag aaccgtggat caccagctgg gttgatcgct ctgccaatgc
    29881 cattggtttg agtctgtata acttcctcaa catcctcaat attaatcaga tttggttgta
    29941 cggtcgcagt tgtgcctttg gtgagaactg gcttaatact attattcgcc agacaggatt
    30001 taacccgttc gaccgcgacg aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca
    30061 attaagccgc gcacaacagg tgctgggaat tggctatttg tatgttgagg cgcagttacg
    30121 acagatttga tggcgcgata acgtagaaag gcttcccgaa ggaagccttg atgatcataa
    30181 acgaaaaatt gcctgatgcg ctacgcttat caggcctaca cggagattgc aatatattga
    30241 atttgcaaga ttttgtaggc ctgagaaggt gttcatgccg catccaacat gaacaacgag
    30301 cacattgtca gcaaatcacc gtttcgctta tgcgtaaacc gggtaacgtg cgcagatgtc
    30361 gagaacttta cctttgatgc gctcgataac ggcttcatca ttgatgctgt ccagcacgtc
    30421 acacatccag ccagccagtt ctttcgcttc ggcttcttta aagccgcgac gggtaatcgc
    30481 cggagtacct acacgaatac cggaggtcac aaacgggctc ttcggatcgt tcggtacgct
    30541 gtttttgttg acggtgatgt tagcacggcc cagagcggcg tctgcttctt taccggtcag
    30601 gtttttatca accagatcaa ccaggaacag gtggttatca gtgccgccgg aaaccacttt
    30661 gtagccgcgc tcgaggaaca cttctaccat cgctttagcg tttttcgcga cctgctgctg
    30721 gtaagttttg aactcaggct ccatcgcttc tttcagagca accgctttac cggcgattac
    30781 gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt ttttgtacag
    30841 ctcttcgcta ccacctttcg ccaagatcag gccgccgcgc ggacccgcca gggttttgtg
    30901 agtggtggta gtaacaacgt gagcatgagg aaccgggttc gggtagacgc cagcagcaac
    30961 caggcccgca acgtgcgcca tatcaacgaa cagataagca ccgatgctgt cagcgatttc
    31021 acgcattttc gcccagtcaa ccacgccgga atatgcagag aaaccaccga taatcatttt
    31081 cggcttgtgt tctttggctt gtttttccag atcggcgtaa tcgatatgac cggtagcatc
    31141 gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag aaccgtgagt
    31201 caggtgaccg ccatgcgcca ggttcatacc cagaacggta tcacctggtt ccagcagcgc
    31261 ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag cgtagtcagc
    31321 gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa catactcgca
    31381 accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca gctgagaacc
    31441 ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt cgatgtgctc
    31501 ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat aatcggcaat
    31561 gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa atttttggcc
    31621 tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg tgcgataaca
    31681 ggtcttgaca aaggaattta cgcaaacgat taccttcagg ctacgcaagg ctttggagaa
    31741 taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa tttttcactt
    31801 catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct attttttata
    31861 agatgcattt gagatacatc aattaagatg caaaaaagga agaccatatg cttgacgctc
    31921 aaaccatcgc tacagtaaaa gccaccatcc ctttactggt ggaaacgggg ccaaagttaa
    31981 ccgcccattt ctacgaccgt atgtttactc ataacccaga actcaaagaa atttttaaca
    32041 tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt taacgctatt gccgcctacg
    32101 ccagtaatat tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc gcgcagaagc
    32161 acaccagctt ccagatcaaa ccggaacagt acaacatcgt cggtgaacac ctgttggcaa
    32221 cgctggacga aatgttcagc ccggggcagg aagtgctgga cgcgtggggt aaagcctatg
    32281 gtgtactggc taatgtattt atcaatcgcg aggcggaaat ctataacgaa aacgccagca
    32341 aagccggtgg ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca ccgcgcagcg
    32401 cgcttatcac cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca gaataccgtc
    32461 cggggcaata tctcggcgtc tggctgaagc cggaaggttt cccgcatcag gaaattcgtc
    32521 agtactcttt gactcgcaaa ccggatggca aaggctatcg tatcgcggtg aaacgcgaag
    32581 agggtgggca ggtatccaac tggttgcaca atcacgccaa tgttggcgat gtcgtgaaac
    32641 tggtcgctcc ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca gtgacgttaa
    32701 tctctaccgg tgttggtcaa acgccaatgc tggcaatgct cgacacgctg gcaaaagcag
    32761 gccacacagc acaagtgaac tggttccatg cggcagaaaa tggcgaagtt cacgcctttg
    32821 ctgatgaagt taaggaactg gggcagtcac tgccgcgctt taccgcgcac acctggtatc
    32881 gtcagccgag cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt ctgatggatt
    32941 tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc tgcggcccgg
    33001 ttggcttcat gcagtttacc gcgaaacagt tagtggatct gggcgtgaag caggaaaaca
    33061 ttcattacga atgctttggc ccgcataagg tgctgtaatt tgatgttgcc ggatggaaac
    33121 atccggcaac ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg gtacggatgc
    33181 gaatgacccg agccacgtca aagacgaaga ttttaccgtc accgattttg ccggtttgcg
    33241 ccgtgcgaat aatggtatcg acacaggtat cgacaatgtc gtccggtacg acaatctcaa
    33301 ttttcacttt cggcagaaaa tccaccatat actccgcgcc gcggtacagc tcggtatggc
    33361 ctttctggcg accaaagcct ttcacttcgg tcaccgtcat gccggtaata ccgacttcgg
    33421 ccagtgcttc gcggacatcg tccagcttga agggttttat aatcgcatca atctttttca
    33481 tgctattcct tgaaaaggtt gcctgtcttt tgatctgcta aacgtaacac ataacgccaa
    33541 ttcattcctt gaaatcgttt gcatccagct cgtgtcggga aagcagttta taaaattctg
    33601 tccggttgcg ccccgccatt ctcgccgcgt gggtgacgtt gcctttggtg atttgcagca
    33661 gcttacgcaa gtagttgagt tcaaaatgat tacgtgcctc aacaaaggtt ggcagcgccg
    33721 tattttcacc ctccagcgcc tgctccacca gcgcatcact aatcaccgga gatgaggtca
    33781 gcgccacgca ctgttcaatc acgttgacca actggcgcac attacccggc cagctcgcgg
    33841 tcatcaggcg tttcatcgca tcggtagaga acgcgcggac aaacggttta tgtcgctctg
    33901 ccgcctggcg caacaggtga tttgccagta gcggaatgtc ttctgtgcgc tccgccagtg
    33961 ccggaatttt caggctgaca acgttgaggc ggtaatagag atcttcgcgg aattccccgc
    34021 gcgccatcgc ttttgacaga tcacggtgag tggcagaaat aatccgcaca ttgatatcaa
    34081 tatcgcggtt actgcccagc gggcgcactt tacgctcctg caacacgcgc agcagtttga
    34141 cctgtaacgg cgcaggcata tcgccaattt catcgagaaa tagcgttccg ccttccgccg
    34201 cctggaataa accttcgcga ttgctgacag cgccagtaaa cgcgccacgc gcatgaccaa
    34261 acagctccga ctccagcaat tgctcgggca atgcgccaca gttaatagca ataaatggtt
    34321 tgctgttgcg cgggctggcg ttgtggatag cctgggcgaa aatctctttc ccggtgccgc
    34381 tctgaccgtt aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc tgttccagca
    34441 aacgcagcat cagcgggctg cgggtgacaa ttgcctcgcg ccagcgttca tcggtggctg
    34501 gcgcggattg ctccagcgca tcgtcaattg cctgatatag cgcatcttta tcgacaggct
    34561 tggtgaggaa actaaaaacg ccctgctgtg ttgcagcaac ggcatcgggg atagaaccat
    34621 gcgcagtaag aataattact ggcattcccg gctgcacttt ctggatttca gcaaatagct
    34681 gcataccgtc catttcatcc atccgcaggt cgctgataac taaatctact ttttcgcgat
    34741 tcagtacccg taatccttca gcgccacttt ccgccgtgac cacactgtag ccttcgctgg
    34801 tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat aataaatgcg
    34861 caggtttatg gctcatcagg agtgacctca tgggtggatg gcgcgggctt ttcactctcg
    34921 tgcggcgtat ccggcgagaa atttccagcc ggtttgcggg tcgagagctg gcgttcaata
    34981 tcggtcaggt tttccagctt gcgggtggtg agttccagtt gctgctgtag aacgtgatgt
    35041 tgctggcgca atgtatccag ctcgctgtcg ctggactgct ggagtttact gtaacgttgg
    35101 cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg atacagtggg
    35161 cgaacctgtg ccgggatctc ggtacttaac gcttcaatac gcgcgaccag ctggcggcgc
    35221 tcatacggcg taattttggc gtcggcgagc aaaatccctt gtttaaaggt attttgccag
    35281 ctgccgtcgt catattggcg agcttgctga cgcgactgcg caggcattaa acgatcagca
    35341 caatccatcg cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc ttgcagcgcc
    35401 cagatgtcgc tacattcagt agaaagatag tcagccagtt gataaaccgg aattttttct
    35461 tctgctggcg tatcaatggc tggcttattg tgattctgca cgcaacccag caatgccaga
    35521 catggcagcc ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat gtgtcgcata
    35581 ttcaccagac ttaaagccta tcccagtggg cgtaattgtt gcagacagtc tggacatgga
    35641 cagcgcggag aaaccggggc gtacatatcg tacgtgaaga tttcgagcac tacccggggc
    35701 cgaactgaca aataaaatag cctgatagga taggctgtta gcattatttc gtgtttttcg
    35761 acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc agatacagtt
    35821 ccccttgcat acggcgaata caatccctgg caatgcttaa tcccagaccg ctgcccttca
    35881 ccgccccttt tcgctggtgg cttccctgaa aaaagggttc gaagatcatg gcgcgttcct
    35941 cttgcggaat gggcgtgcct gtattgatga catcaatata aacccgcgca ccatgtaaac
    36001 tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg caccgcattg gagtaaagat
    36061 tatccagtac gctcatcagc agcattggct ccgccaggca agctgttgct ttgagatcga
    36121 cgtcggtatg catcatttta gcccgtgcgg gcaggctatg agcagaaacc actgtctcca
    36181 ccagcggtgc taattcaaca ttctccagtt ccaccgcact gtccgcctgt ttacggttgt
    36241 aatcaagcag ttgttcgatc agtttttgca aattgcagct gctgctatca agaatgctca
    36301 ccacctcttt ttgctctggc gtaagcggcc cgacaacctg atcagccagt aattcagtgc
    36361 cctcgcgcat actcgccagt ggcgttttta attcatgaga taaatgtctt aaaaattgat
    36421 ggcgttggga ttccagccat gacaggcgct cacttaacca aagaatacgt tgcccaaccg
    36481 agcgtaactc gctcggtcca cagaacgaaa cgctattgcc cagagaacgc ccttccccca
    36541 gacggttgat catgcgctcg atatttttca ccggcccgat aatcatccgc gtgaaaagta
    36601 gcaccatcac cagactcacc agaaatagca ccagcgattg ccaaccaaaa tattgcccac
    36661 gttcggcgat ttcacgctga agttgctgcc cacgagagaa caccactgtg cgcgtggcct
    36721 gcaccatttc ggtattggca ctggcaaagg cttcaagacg cgcggcggcg gcagcatcgg
    36781 gaccactgtt gttacactga agttgggcca gattgttcaa atcctgacgc aatgcctggt
    36841 aaagtttatc gtctggcagt acgcctgcgt gggcatcgag catttcgctg taacgcttgc
    36901 gctggctttg ataaaccttc gcaagcgtag ggtcgtccag cacgcaatac tgacggtagc
    36961 tacgctccat ctccagcgcc gcgttggtca tcgcttcact acgccgggca tcaataagcg
    37021 tagtgcggtt aaccagcgcc gcctgatcgc taagcgcatt caggctttgc caggcttgcc
    37081 atgccagcac caacaggggt agtagaatta gcaaaaatgc cagcattacc agttgtcgta
    37141 atgagcgggg aaaaacgggc cagcgtttca aggtgttact ctcgtcagac gcgaatagcc
    37201 tgatgctaac cgaggggaag ttcagataca acaaagccgg gaattacccg gctttgttat
    37261 ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt tgatatagct acgctgatat
    37321 cagaagctgg acggcaggca ccttgttgtg cgtcattcga attttatgta gcacgtcccg
    37381 aaggggctga cataagtcgg tgaatgagcc actgcttact attatgcagt aactgtgcca
    37441 ataaagaaaa tagtttggta acgtactgat tatacgttgc tttgagggat ttatggcttc
    37501 ttcgctattt gaatgtaaat cagccactgt gtcgctaaaa agagacaact taagataaac
    37561 ttattagatt gcaataaaat caatcattta agtgtcgcca gaaagcgaca cggtaaacca
    37621 cccattgtcg tgatttacag acacaaaaaa gctcccggag ttgggagctt atgatagtgg
    37681 ttggtgctta atgccgcatc cgggctgcaa aaccaatgaa ctgatgactt accccaactg
    37741 cttacgcgca ttgcggaaaa tgcgcatcca tgggccatcc tcgccccagt tttccggatg
    37801 ccaggagttg ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa tggtgactcg
    37861 accactttca gtcgtaaccg ccgtaatacc gttcggtgaa ccgttcgggt tagccgggta
    37921 ggtttcagtg actttgccga agttatcgac atagcgcagt gccaccagcc ctttgctttc
    37981 cagtgccgcc agatgcgccg catcacgcac ttccacgcgc ccttcaccat gagagacagc
    38041 aatcggcatt tgcgagccca ccatcccctg caacagcaga gacgggcttt gggttacttc
    38101 aaccaggctg aaacgcgctt caaagcgatc ggaggtgtta cgcacaaaac gtggccacaa
    38161 ctcactcccc gggatcagtt cacgcaggtt agacatcatc tggcaaccgt tacatacccc
    38221 cagggccagc gtttgcggac gatggaagaa ggttgcaaac tcatcgcgta cacggtcatt
    38281 gaacaggata gacttcgccc aaccttcacc ggcacccagc acatcaccgt aggagaaacc
    38341 tccgcacgcg accagggcgt gaaaatcctc cagtcccgta cgtccagtca gcaggtcgct
    38401 catatgcacg tcgatagcat caaagccccc acgttggaaa gctgccgcca tttcaacatg
    38461 cgagttcacg ccctgctcac gcagtacggc aactttcgga cgtgcgccag tggcaatata
    38521 cggtgctgcc acatcttcgt tgatatcgaa cgacagtttt acattcaggc ccggatcggc
    38581 gtcgttagat ttcgcctgat gctcctgatc ggcacactcc gggttgtcac gcaggcgctg
    38641 catctgccag gtagtttctg cccaccagac acgcaacgta gtgcggcttt cgctgaatac
    38701 agtctgcccg ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc caacataatg
    38761 gacgcaatcc gcaagcccat gctgtgccag tacggactcg accgcttcac ggtcagcggc
    38821 acgaacctga atcaccgcac ccagttcttc gttaaacaac gccgccaggc gatcgtcacc
    38881 cagagaggcg atatccgcgt caatgccaca atgacctgca aaggccattt ccgccagcgt
    38941 taccagcagg cctccatcag agcggtcgtg atacgccagc agcttacgct gtgcaaccag
    39001 cgcctgaatc gcgtcataga agcctttcag ttggacaaca tcgcgtacat ctgccggttt
    39061 gtcgccaagc tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt tattgccttt
    39121 gcccaaatca atcagcagca gtgcgttatc ttcggtacaa agctgcggcg tgatggtgtg
    39181 acgtacatct tccacgcggg caaatgcaga aatcaccagc gacagcggcg acgtcatttc
    39241 gcgctcttcg ttaccttcct gccagcgggt tttcatcgac atagagtctt tacccaccgg
    39301 gatcgtcagg cccagcgccg gacaaagttc ttcacccacg gctttaacgg cttcatacag
    39361 gcccgcatct tcaccagggt ggcctgccgc cgccatccag ttggcggaaa gtttaatgcg
    39421 tttgatatcg ccaatctgtg ttgcggcaat attggttaac gcttcaccga ccgccagacg
    39481 ggcagaggcg gcgaaatcca gcagcgcaac cggcgcacgc tcgccaatcg ccatcgcttc
    39541 accgtagtag ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg gcacctgcca
    39601 cggccccacc atctgatcgc gcgccaccat gccggttacg ctgcggtcac cgatggtcac
    39661 caggaaagtt ttctccgcca cggtcggcag atgcagtacg cgtttcaccg cgtctgcaat
    39721 ggtgatccct tcacggacca gcgcatcgcc cttcgctttc agcgtttgta catcgcgggt
    39781 catcttcggc gttttaccaa gcaggacgtc cagcggcaga tcgatcggct gattatcaaa
    39841 atgacgatcg tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca ccgcgtaggg
    39901 tgcgcgctca cgcttacaca gttcgtcaaa cagcggtaat tgatcggcag caaccgccag
    39961 cacgtagcgt tcctgggatt cgttacacca gatttccagc gggctcatgc ccggttcgtc
    40021 gcttagaata tcgcgcagtt caaatttacc gccgcgtccg ccgtcgctca ccagctccgg
    40081 catcgcgtta gaaagaccgc ccgcaccgac gtcgtggata aacagaattg ggttagcatc
    40141 accaagctgc cagcaacggt cgatcacttc ctggcaacga cgctccatct ccgggttgtc
    40201 gcgctgtacg gaagcaaagt cgaggtcggc atcagactga ccagacgcca tggaagacgc
    40261 cgcgccgccg ccaagaccga tgttcattgc cgggccgccg agaacgacca gcttcgcacc
    40321 gacgttgatc tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc cgcccgccag
    40381 catgatcggt ttgtgataac cgcgcagctc ttcgccattg tggctgttca ctttttcttc
    40441 ataagtacgg aagtagccgt tcagtgccgg acgaccaaat tcgttgttaa acgccgcacc
    40501 gcccagcggg ccttcggtca tgatgtccag cgcggtaaca atgcgctcag gcttaccgaa
    40561 atcttcttcc cacggctgtt cgaagccagg aattcgcagg ttggatacgg agaaaccaac
    40621 caaaccagct ttcggcttcg cgccgcgccc ggtggcacct tcatcgcgga tttcaccgcc
    40681 ggaaccggtc gccgcgcccg gccacggaga aatcgccgtc gggtggttgt gagtttcgac
    40741 tttcatcagg atgtgcgccg gttcctgatg gaaattgtag cggcccgttt cgtggtcagc
    40801 aaagtagcgg cccacttcag aaccttccat tacggcggcg ttatctttat aagcagagag
    40861 aacgtgatct ggcgtggttt cgaaagtatt tttgatcatc ctgaacagcg atttcggctg
    40921 ttgttcaccg tcgataatcc agtcggcgtt aaaaattttg tggcggcagt gctcagagtt
    40981 cgcctgagcg aacatataca gctcgatgtc gttcgggtta cgaccaagct ttgtgaaagc
    41041 atcctgcaga tagtcaattt catcttccgc cagagccaag ccaagacgca ggttagcgtc
    41101 gatcagcgcc tgacggccct gccccagcaa atcaacgctt gtgaccggag tcggttgatg
    41161 gtgggcaaac aactgctctg catcatctaa atcaaaaaag accgtttcca tcatgcggtc
    41221 gtgcagttca gcggtaacct gctgccattg ttcattggtc agcgtaccgg cttctatata
    41281 gtaagcaacg ccgcgctcaa ggcggtttac ctgttgtagc ccgcagttat gggcaatatc
    41341 ggtcgctttc gaagaccagg gagagatggt gccaggacgc ggggtcacca gcaggagttt
    41401 gccttgcggg gcgtggctgg cgagtgccgg accatatttc agcaggcgtt caagttgtgc
    41461 gtgctcatca tcgtttaacg gcgcattgag gtcagcaaaa tggacatact cggcgtaaat
    41521 attgtgaacc gggagcctgg cagcctgaaa acgtgccagc agtttgttga ttcggaatgc
    41581 cgacagtgca ggcgaaccac gcagaatttc catcataagt ctctcgtctt ctaagctttc
    41641 ggtgtaccca agggggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac
    41701 cgtttgcgtg gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta
    41761 ataagttgcc atctggcgca ggtttacgca aaatgccgct catttatgag taaacctttc
    41821 actattatta cgttttttca agctgggacg cgcacgacac agagaattaa ctaattgaaa
    41881 aaattaaaga ttaattatct gttcatcggc attctggcac tgctgctcgc ggtcgctctc
    41941 tggccatcca ttccctggtt tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg
    42001 ggagagttgc gtgtgagcac cattcatact cccctgactt ataacgaaat caacgggaaa
    42061 ccttttggcc tggattacga actggcgaaa cagtttgccg attacctcgg cgtaaaactg
    42121 aaagtgaccg tgcggcagaa tatcagccag ctgtttgacg accttgataa tggtaacgcc
    42181 gacctgctgg cggcaggact ggtctataac agtgagcggg taaaaaatta tcagcctggc
    42241 cctacctatt attccgtgtc acaacaactg gtttataaag tgggtcagta tcgcccacgt
    42301 acgctgggca acctgacggc ggagcaactc accgttgcac cgggtcatgt ggtagttaac
    42361 gatctccaga ccctgaaaga aacaaaattc ccggaattaa gctggaaggt agacgacaaa
    42421 aaaggctctg cggaattaat ggaagatgtc atcgaaggaa aactcgatta caccattgct
    42481 gattctgtcg ccatcagcct gtttcagcgc gttcacccgg agctcgccgt agcgctcgat
    42541 atcaccgatg aacaaccggt gacttggttt agcccgttag atggcgataa taccctttcc
    42601 gccgccctgc tcgacttctt caacgaaatg aatgaagacg gtacgctggc acgcattgaa
    42661 gagaaatacc tggggcatgg cgatgatttt gattacgtcg atacgcgcac atttttacgc
    42721 gccgtcgatg cggtactgcc gcagttaaag cccctgtttg agaaatacgc cgaagaaatt
    42781 gactggcgtt tgctggccgc tattgcttat caggaatcgc actgggatgc acaggccact
    42841 tcaccgacgg gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca aagcctcggc
    42901 attacggatc gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat
    42961 atgatgagta aagtgccgga aagtgtgccg gagaacgaac ggatctggtt tgccctcgct
    43021 gcgtacaata tgggctatgc gcatatgctg gatgcccgcg ccctgacggc aaaaaccaaa
    43081 gggaatcctg acagttgggc tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc
    43141 tattacagca agctgactta cggctacgct cgtgggcatg aagcctacgc ttatgtcgaa
    43201 aatattcgta agtatcagat tagcctggtg ggttatctgc aagagaaaga gaagcaggct
    43261 acagaagcgg cgatgcaact ggcgcaggat tatccggcgg tatcgcctac ggagttgggc
    43321 aaagagaaat ttccttttct ctcgtttctt tcccagtcgt catcaaacta tttgacccat
    43381 tctccctctc tgctgttttc caggaaaggg agtgaagaga aacaaaatta atccgtcgag
    43441 gattgcgctt ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc
    43501 aacgccgcgc actcatccgc cagtattcct tccgtaattt ccactcggtg attcataccc
    43561 ggatgatgca gcacatccat taaagatccc gcagcgccag ttttcgcgtc acgcgcacca
    43621 aagaccacgc gaccaatgcg actgtggatc atcgctccgg cacacattac acatggttca
    43681 agcgtgacat acaacgtggc gtcgatcaga cgataatttt gcatcaccag accaccctgc
    43741 cgcagggcca tgatttctgc atgtgcggtg ggatcatggc gaccaatcgg gcggttccag
    43801 ccttcgccga ttacccgatt gttatgcact aataccgcgc cgaccggcac ttcccgctca
    43861 tcccaggcac gtttcgccag cgtcagcgcg tgacgcatcc aatattcgtg gctaaattcg
    43921 acttcagaca aaaagaaaac tccggttata aaagcgcggc gcattataca cggacgctat
    43981 gctttactct attccagttg ctggagttca ccgcgcgggg ttactcgcca acgatgctga
    44041 cagaaataaa gcagcggatt gtcctgatta ctgtcgctat agccactgta cagccgcagc
    44101 ggcgtgccga ttttgcgctc cagttgtgcg accttttcat gtcccagaca acgcatcgtc
    44161 aatacccaac caccatagcc acgctgaatt tggctggcga taagattaac ccgcggcagc
    44221 cagggcgtat cgaaataaac cgcttcaacc agcggctgcg gagagccggt aatcaaccag
    44281 atatcagcat cggaacttaa caggtaggtg gttaatcgct cctgaaccag cggaaaggcg
    44341 gtaacattgt cgcgaaacca gcgcacgaaa tcggcctgca acgtctgtaa acgtgcttcg
    44401 ctgtgaccaa aagtgcaccc ccacagaagc agactcatcg gccagcgtgc cgcacgacct
    44461 tttatcaata acgcaatggc tataatcggt aacaacggca ggacaagtaa cgcattcagc
    44521 ggttggcgac gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt
    44581 ccatctaagt caaaaaacac cacacgacgc tcgtgagttg ccaaaccata ctcctctgga
    44641 ctgaaacatc ctgattaatt ttcgctacta catagcctaa cagatagatc atcacttttc
    44701 cggcaacaat cgaccgcatc atggctaact ggaattttta attcatggca attagcggca
    44761 atggaatata aaattcactc gcgtgtgtct catattgcct gttgtcgcca ctatttcatc
    44821 gcgaggaaag atatgaacgg cttacttcgt atccgtcagc gttaccaagg gcttgcccag
    44881 agtgataaaa aactggcgga ttatctgctg ctacaacctg atacggcgcg ccatttaagc
    44941 tctcagcaac tggccaacga agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa
    45001 aaactcggct ataaaggttt tccggcgctt aagttggcgt tgagtgaagc gctggcaagc
    45061 cagccggaat cgccctccgt gcccattcat aaccaaatcc gcggtgatga tccgttacgg
    45121 ctggtcggcg aaaaactgat taaagaaaat accgccgcta tgtacgcaac gctaaacgtt
    45181 aatagtgaag agaaactgca tgaatgcgta acaatgttgc gctctgcgcg gcggataatt
    45241 ctgaccggta ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag
    45301 attggcttca atgctgccgc actgcgcgat atgcatgcgc tactcgcaac agtacaggcg
    45361 tcgtcccctg acgatctgtt attagccatt tcctacaccg gtgtacgacg cgagttaaac
    45421 ctggcggcag atgagatgct gcgagtgggc ggaaaagtgc tggcgattac cggctttact
    45481 ccgaatgccc tgcaacagcg tgcttctcat tgcctgtata ccattgccga agaacaagcg
    45541 acaaacagtg cttcaatctc tgcttgtcac gctcagggaa tgttaacgga tttgctgttc
    45601 attgcgctga ttcagcagga tctggaactg gcaccagaac gtattcgtca tagtgaagcg
    45661 ctggtgaaaa aactggtctg agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg
    45721 ttttgagagt ttccttatgg cgctgttaat cactaaaaaa tgcatcaatt gtgatatgtg
    45781 tgaaatcaaa taaaaacaca tttattttca tagtgataca gcaatgtgtt attgaatgtg
    45841 ttgttggatt tatggtgcaa aaaaattttg cataagttca gcaacacaaa gaacaccact
    45901 ttctttaacg tcctccgcgc cctaaaggac ggggctttac ggcgcaaaag gcctgtgaca
    45961 tacaacatta acttttcagt caaccgtagc acgttcttgt atacgccgtg tctgcggttt
    46021 tatataaccc tggctggaaa cctcttatac aaagtcgatt cgccaacatc atagatgatc
    46081 gccaccttct ggcgagaaac tcctgatgca attagtcgcc cggcctgcgc ccattattct
    46141 ggtgaaagtt tgggacggcg accgccaatt cgtccctgtg cgcgagcagc ttccagtcct
    46201 gcttttgttc gttcaacaat cagttcacgt tccatttcag ccagggcacc catcacatgg
    46261 aagaaaaagc gtcccattgg tgtgctggta tcaattgaat cagtcagact acgaaagttg
    46321 atgcctcgtt cgcgcaacgc ctccaccagc acgacaagat gccgcatact gcgccccagc
    46381 cgatccagct tccagacaac cagtgtgtcg cctgccgata atgtcttgag cagttttttc
    46441 agccccggtc tttcggactt tgtaccactt atcttgtctt caaaaatcag ctcgcatcct
    46501 gcacagttca acgcattacg ttgtagatcg gtattctgg
//
LOCUS       DAZOZX010000018        45314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZOZX010000018 DAZOZX010000000
VERSION     DAZOZX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.018"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(177..1841)
                     /gene="glnS"
                     /locus_tag="ACHUPT_000964"
     CDS             complement(177..1841)
                     /gene="glnS"
                     /locus_tag="ACHUPT_000964"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0818643.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2044..3990)
                     /gene="nagE"
                     /locus_tag="ACHUPT_000965"
     CDS             complement(2044..3990)
                     /gene="nagE"
                     /locus_tag="ACHUPT_000965"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0818644.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4323..5123
                     /gene="nagB"
                     /locus_tag="ACHUPT_000966"
     CDS             4323..5123
                     /gene="nagB"
                     /locus_tag="ACHUPT_000966"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0818645.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5183..6331
                     /gene="nagA"
                     /locus_tag="ACHUPT_000967"
     CDS             5183..6331
                     /gene="nagA"
                     /locus_tag="ACHUPT_000967"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0818646.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6340..7560
                     /gene="nagC"
                     /locus_tag="ACHUPT_000968"
                     /gene_synonym="nagR"
     CDS             6340..7560
                     /gene="nagC"
                     /locus_tag="ACHUPT_000968"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0818647.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7608..8360
                     /gene="nagD"
                     /locus_tag="ACHUPT_000969"
                     /pseudo
     CDS             7608..8360
                     /gene="nagD"
                     /locus_tag="ACHUPT_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8411..8509)
                     /locus_tag="ACHUPT_000970"
                     /pseudo
     CDS             complement(8411..8509)
                     /locus_tag="ACHUPT_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8757..10421
                     /gene="asnB"
                     /locus_tag="ACHUPT_000971"
     CDS             8757..10421
                     /gene="asnB"
                     /locus_tag="ACHUPT_000971"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0818648.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10800..10876
                     /locus_tag="ACHUPT_000972"
     tRNA            10800..10876
                     /locus_tag="ACHUPT_000972"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10834..10836,aa:Met,seq:cat)
     gene            10885..10969
                     /locus_tag="ACHUPT_000973"
     tRNA            10885..10969
                     /locus_tag="ACHUPT_000973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10919..10921,aa:Leu,seq:tag)
     gene            10993..11067
                     /locus_tag="ACHUPT_000974"
     tRNA            10993..11067
                     /locus_tag="ACHUPT_000974"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11025..11027,aa:Gln,seq:ttg)
     gene            11102..11176
                     /locus_tag="ACHUPT_000975"
     tRNA            11102..11176
                     /locus_tag="ACHUPT_000975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11134..11136,aa:Gln,seq:ttg)
     gene            11192..11268
                     /locus_tag="ACHUPT_000976"
     tRNA            11192..11268
                     /locus_tag="ACHUPT_000976"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11226..11228,aa:Met,seq:cat)
     gene            11317..11391
                     /locus_tag="ACHUPT_000977"
     tRNA            11317..11391
                     /locus_tag="ACHUPT_000977"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11349..11351,aa:Gln,seq:ctg)
     gene            11429..11503
                     /locus_tag="ACHUPT_000978"
     tRNA            11429..11503
                     /locus_tag="ACHUPT_000978"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11461..11463,aa:Gln,seq:ctg)
     gene            complement(11657..12832)
                     /gene="ubiF"
                     /locus_tag="ACHUPT_000979"
     CDS             complement(11657..12832)
                     /gene="ubiF"
                     /locus_tag="ACHUPT_000979"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0818649.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12978..14402
                     /gene="miaB"
                     /locus_tag="ACHUPT_000980"
     CDS             12978..14402
                     /gene="miaB"
                     /locus_tag="ACHUPT_000980"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0818650.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14555..15595
                     /locus_tag="ACHUPT_000981"
     CDS             14555..15595
                     /locus_tag="ACHUPT_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0818651.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15592..16059
                     /gene="ybeY"
                     /locus_tag="ACHUPT_000982"
     CDS             15592..16059
                     /gene="ybeY"
                     /locus_tag="ACHUPT_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0818652.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16149..17027
                     /gene="corC"
                     /locus_tag="ACHUPT_000983"
     CDS             16149..17027
                     /gene="corC"
                     /locus_tag="ACHUPT_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0818653.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17052..18590
                     /gene="lnt"
                     /locus_tag="ACHUPT_000984"
     CDS             17052..18590
                     /gene="lnt"
                     /locus_tag="ACHUPT_000984"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0818654.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18987..19895
                     /gene="gltI"
                     /locus_tag="ACHUPT_000985"
     CDS             18987..19895
                     /gene="gltI"
                     /locus_tag="ACHUPT_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0818655.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            20065..20805
                     /gene="gltJ"
                     /locus_tag="ACHUPT_000986"
     CDS             20065..20805
                     /gene="gltJ"
                     /locus_tag="ACHUPT_000986"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0818656.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20805..21479
                     /gene="gltK"
                     /locus_tag="ACHUPT_000987"
     CDS             20805..21479
                     /gene="gltK"
                     /locus_tag="ACHUPT_000987"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0818657.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21479..22204
                     /gene="gltL"
                     /locus_tag="ACHUPT_000988"
     CDS             21479..22204
                     /gene="gltL"
                     /locus_tag="ACHUPT_000988"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0818658.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22322..23257
                     /gene="rihA"
                     /locus_tag="ACHUPT_000989"
     CDS             22322..23257
                     /gene="rihA"
                     /locus_tag="ACHUPT_000989"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0818659.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            23341..25011
                     /gene="hscC"
                     /locus_tag="ACHUPT_000990"
                     /pseudo
     CDS             23341..25011
                     /gene="hscC"
                     /locus_tag="ACHUPT_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(25071..26522)
                     /gene="djlC"
                     /locus_tag="ACHUPT_000991"
     CDS             complement(25071..26522)
                     /gene="djlC"
                     /locus_tag="ACHUPT_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0818660.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEEESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26519..27226)
                     /locus_tag="ACHUPT_000992"
     CDS             complement(26519..27226)
                     /locus_tag="ACHUPT_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0818661.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27328..27882
                     /locus_tag="ACHUPT_000993"
     CDS             27328..27882
                     /locus_tag="ACHUPT_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0818662.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27892..29319)
                     /locus_tag="ACHUPT_000994"
     CDS             complement(27892..29319)
                     /locus_tag="ACHUPT_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0818663.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29316..30023)
                     /locus_tag="ACHUPT_000995"
     CDS             complement(29316..30023)
                     /locus_tag="ACHUPT_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0818664.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            30187..31164
                     /gene="ybeQ"
                     /locus_tag="ACHUPT_000996"
     CDS             30187..31164
                     /gene="ybeQ"
                     /locus_tag="ACHUPT_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR0818665.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(31234..31716)
                     /locus_tag="ACHUPT_000997"
     CDS             complement(31234..31716)
                     /locus_tag="ACHUPT_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0818666.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            31951..34533
                     /gene="leuS"
                     /locus_tag="ACHUPT_000998"
     CDS             31951..34533
                     /gene="leuS"
                     /locus_tag="ACHUPT_000998"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0818667.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            34548..35129
                     /gene="lptE"
                     /locus_tag="ACHUPT_000999"
     CDS             34548..35129
                     /gene="lptE"
                     /locus_tag="ACHUPT_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0818668.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            35129..36160
                     /gene="holA"
                     /locus_tag="ACHUPT_001000"
     CDS             35129..36160
                     /gene="holA"
                     /locus_tag="ACHUPT_001000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0818669.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            36162..36803
                     /gene="nadD"
                     /locus_tag="ACHUPT_001001"
     CDS             36162..36803
                     /gene="nadD"
                     /locus_tag="ACHUPT_001001"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0818670.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            36827..37438
                     /locus_tag="ACHUPT_001002"
     CDS             36827..37438
                     /locus_tag="ACHUPT_001002"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR0818671.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            37698..38015
                     /gene="rsfS"
                     /locus_tag="ACHUPT_001003"
     CDS             37698..38015
                     /gene="rsfS"
                     /locus_tag="ACHUPT_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0818672.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            38019..38486
                     /gene="rlmH"
                     /locus_tag="ACHUPT_001004"
     CDS             38019..38486
                     /gene="rlmH"
                     /locus_tag="ACHUPT_001004"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0818673.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            38517..40418
                     /gene="mrdA"
                     /locus_tag="ACHUPT_001005"
     CDS             38517..40418
                     /gene="mrdA"
                     /locus_tag="ACHUPT_001005"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0818674.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            40421..41533
                     /gene="mrdB"
                     /locus_tag="ACHUPT_001006"
                     /gene_synonym="rodA"
     CDS             40421..41533
                     /gene="mrdB"
                     /locus_tag="ACHUPT_001006"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0818675.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            41544..42632
                     /gene="rlpA"
                     /locus_tag="ACHUPT_001007"
     CDS             41544..42632
                     /gene="rlpA"
                     /locus_tag="ACHUPT_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0818676.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            42771..43982
                     /gene="dacA"
                     /locus_tag="ACHUPT_001008"
     CDS             42771..43982
                     /gene="dacA"
                     /locus_tag="ACHUPT_001008"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0818677.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            44093..44356
                     /gene="ybeD"
                     /locus_tag="ACHUPT_001009"
     CDS             44093..44356
                     /gene="ybeD"
                     /locus_tag="ACHUPT_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0818678.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            44457..45098
                     /gene="lipB"
                     /locus_tag="ACHUPT_001010"
     CDS             44457..45098
                     /gene="lipB"
                     /locus_tag="ACHUPT_001010"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0818679.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT        11278 a        11583 c        11506 g        10947 t
ORIGIN      
        1 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca atatattgca
       61 gagatcatgt aggcctgata agcgaagcgc atcaggcaat ttagcgtttg aaatttattg
      121 tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac ttaaaattac
      181 tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcg
      241 gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta
      301 cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc
      361 gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct gaacagacga
      421 tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct
      481 ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag
      541 atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat gacgtaagca
      601 ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg
      661 cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg gctccccatt
      721 tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg atagttttcg
      781 ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gcgcattttc gttgagatct
      841 tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt ggtcacgccg
      901 atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg cagaccggaa
      961 atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc
     1021 agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc
     1081 ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg gttgtcctgg
     1141 aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat gcaatgggtg
     1201 aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta
     1261 atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc gattttcgca
     1321 cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccaag
     1381 ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt cagggtaccg
     1441 cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc tttattgatc
     1501 agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca
     1561 gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata ctcgatatct
     1621 tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg
     1681 atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc
     1741 ggcgggaaac gggtgtgtac tgtgatgtgc ttaccactgg ccagatcttc atcgatgatc
     1801 tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt
     1861 aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact gttagttttt
     1921 taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga
     1981 agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca ggcataaagc
     2041 agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt
     2101 aatgagcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg
     2161 ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg
     2221 cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc
     2281 catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt
     2341 gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac
     2401 cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc
     2461 cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc
     2521 ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg cagcggctac
     2581 cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac
     2641 aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca
     2701 catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg taatacaggc
     2761 atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg ttgccagttg
     2821 agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt cttctttatc
     2881 ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta
     2941 gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc tggcggcagg
     3001 caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc cggcatggat
     3061 acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag
     3121 cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc
     3181 agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc
     3241 gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga agaagccgga
     3301 catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc cgtggaaaac
     3361 cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg
     3421 atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc
     3481 gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg gcggccagac
     3541 gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg gcacaaagcg
     3601 tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac ggttataggc
     3661 tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt
     3721 gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc
     3781 acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt
     3841 atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc
     3901 gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta acgccctacc
     3961 gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc
     4021 tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc tgtaatcaga
     4081 ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgcatcgcaa attaaacgcg
     4141 tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaac
     4201 cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc tgcgcaaatc
     4261 caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta ttgaggtgaa
     4321 taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata
     4381 tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta ctgggcctgc
     4441 cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc
     4501 aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag
     4561 agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc
     4621 cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc gagtgccgcc
     4681 agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta
     4741 acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca
     4801 aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc
     4861 aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc gaagaagtga
     4921 tgattatggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt gaaggttgcg
     4981 tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc atggtgtgcg
     5041 atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag
     5101 cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct cagggcaata
     5161 ttttttaaaa tcggggggca gaatgtatgc attaacccag ggccggatct ttaccggcca
     5221 cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc
     5281 ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc
     5341 cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga
     5401 agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac
     5461 taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt
     5521 tatgcgtgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc
     5581 gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc
     5641 cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc
     5701 ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc
     5761 cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg ggattacctt
     5821 tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg
     5881 cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga
     5941 ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga
     6001 cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata
     6061 ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat
     6121 gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg
     6181 tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg gcacgctcgc
     6241 cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat
     6301 cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct
     6361 cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt
     6421 gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc
     6481 agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag
     6541 caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac
     6601 gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga tctcagcagc
     6661 aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac gctggaacat
     6721 gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct gcgcgagcta
     6781 atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac
     6841 atgccgcata ttcaggtaga aaactggggg ctggtagaag cactggaaga acgttttaaa
     6901 gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt
     6961 gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg
     7021 attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga aattggccat
     7081 attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact
     7141 atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag
     7201 agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc
     7261 gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa aaccatcgcc
     7321 attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat caccgaagcc
     7381 gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct gaaggcgttt
     7441 cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt
     7501 gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa
     7561 tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa
     7621 atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag
     7681 cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc
     7741 cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg
     7801 atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc taggaaggca
     7861 agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca
     7921 ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc tacaactggg
     7981 acatgattca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc
     8041 cggacaccca cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga
     8101 aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg
     8161 cattaaacaa aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta
     8221 ccgatattct ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt
     8281 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg
     8341 ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca
     8401 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
     8461 aattttcatg cttttgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaatc
     8521 gcactttatc agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa
     8581 ttatcgattc atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca
     8641 ccattacgtt ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca
     8701 tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt
     8761 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
     8821 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
     8881 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
     8941 ctctctacaa ccaacaaaaa aaccacgtac tggcggtaaa cggtgaaatc tacaaccacc
     9001 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
     9061 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
     9121 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
     9181 ggatcatccc actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa
     9241 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
     9301 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
     9361 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
     9421 gccatctgat gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa
     9481 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
     9541 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
     9601 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
     9661 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
     9721 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
     9781 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
     9841 ataaagcgcc caacgttaaa gaactgcatg aagagacggt gcgtaaactg ctggccttgc
     9901 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
     9961 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    10021 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    10081 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    10141 tcgacaccct gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc
    10201 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct
    10261 ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg
    10321 cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt
    10381 ctggtcgtgc ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg
    10441 gagaaatccg gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga
    10501 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    10561 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg
    10621 gctgtagggt ggggaaaaac acaaagtaaa caataattga cgaatatagc gccacgctgt
    10681 tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag gttgacgctg
    10741 caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga aaaaaagatg
    10801 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
    10861 cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat tggtagacgc accagattta
    10921 ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat tcaccagaaa
    10981 gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    11041 ctggttcgaa tccaggtacc ccagccatct tcttcgagta agcggttcac cgcccggtta
    11101 ttggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat
    11161 ccaggtaccc cagccatcga agaaataatc tggctacgta gctcagttgg ttagagcaca
    11221 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa ttctgaatgt
    11281 gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc
    11341 accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc aatttattca
    11401 agacgcttac atcgtaagtg cacccagttg gggtatcgcc aagcggtaag gcaccggatt
    11461 ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccacattaaa aaagctcgct
    11521 tcggcgagct ttttgctttt ctgcgttcat tcaatgtcga atgcgatgtt gacacgtctt
    11581 atccttcaat gtcggatgcg acgctgccgc gtcttatccg acctacggct ggcacgcatc
    11641 cggcaatgtt gtaaggctac aaccctaacg catatttcag cgcctgacgt ttcaacacgc
    11701 cagcacgctc tgccgccatt aacccgagat tacgcacaaa acgcagcggc ggcagattat
    11761 tactgaatcc ggcataaaac agatccatac cactttgcat aatgaagtta tccgccatgc
    11821 gccgcatctg gtaacgcttg aggacaggat aactggccca cgcttcaccg tagctgcggg
    11881 cattcaccag aacatcaatc agggcatcga catcacgata accaagattc accccctgcc
    11941 ccgccagcgg atggatggta tgcgcggcat cgcccaccag cgcaagcccc ggctgcacat
    12001 actgcaacgc atgtcggcgc gtcagcggaa acgcaccagc ggcaagcggt gtaacgtaac
    12061 ccagacgcga cgggaaatgc ttcgcgattt ccgcctggag ttgcgccata ttcatattct
    12121 gcaactggcg aatacgcgtc ggagagtcat accacaccag cgatgcccag ttatcaaaca
    12181 acggcagaaa cgcacgcggt ccgtccgggg taaattgctg ccaggtgctg tcgccgggat
    12241 cgttctcaca ctgtacgcta atcaacatgc acgactgcgc atactgccag gcatgaacgc
    12301 caattcccgc catctgccgc acctgagaat ttgcgccgtc ggcaccaatc accagtttcg
    12361 cgcggatcgt ttcaccgccc ttcagttcca gctcctgaag atcattatct cgatgtaatg
    12421 caatcagcga ggttggcaca cgtaacgtta ctttcggatg cgcttccagc gcctgccaca
    12481 acgcctgttg cagcactgtg ttttccacca tgtagccaag caacggcaac tttaattcgg
    12541 ctgcgtcaaa taccacatgt gccgtttccc actcccaggt ttccagtctg cggtaaggat
    12601 ggcaacgcat agcctgtact gcatcccaga cccctaaccc tttaagcaat gataccgaag
    12661 ccgcgctgat cgccgagatc cgcacgtccg gttggctgtc agcgacaaac ggtgcaggtt
    12721 ctgcgtgctc gatcaccgtt accgaaaatc cgtgctgtgc cagccccagc gccagtgcgc
    12781 cgccgaccat tcctccgccg acaatggcaa tttccgttgg ttgatttgtc atggtcaatc
    12841 atcctgttag caatatgctt taagtttaca ggatttttac ccccaagggc tgataacgct
    12901 catactggtc acaacggcag caaagcatta cactatgcgc cctgcattcc tggctactat
    12961 ttcgcaagag caagtcgatg accaaaaaac tccatattaa aacctggggc tgtcagatga
    13021 acgagtacga ttcatcgaag atggccgatc tgctggatgc cacccacggc tatcaactga
    13081 ccgacgtggc ggaagaagcg gatgtgctac tgctgaacac ctgctcaatc cgcgagaagg
    13141 ctcaggaaaa agtcttccat cagttgggtc gctggaaact gttaaaagag aagaatccag
    13201 acctgattat cggcgtcggt ggctgcgtgg catcgcaaga aggcgagcac attcgccagc
    13261 gcgcccacta tgtcgatatt atttttgggc cgcaaacgct gcaccgtctg ccggagatga
    13321 tcaactccgt gcgcggcgac cgcagcccgg ttgtagatat cagcttcccg gaaatcgaga
    13381 agtttgaccg tctgccggaa ccgcgcgccg aagggccgac cgcgtttgtc tccatcatgg
    13441 aaggctgcaa taaatattgc acctactgcg tggtgcctta cacccgtggt gaagaggtaa
    13501 gccgtccgtc tgacgatatt ctgtttgaga ttgcccagct tgcggctcag ggcgtgcgtg
    13561 aagtcaacct gctcggtcag aatgtgaatg cctggcgtgg tgagaactac gacggcacca
    13621 ccggatcgtt tgccgatctg ttgcgtctgg ttgctgcgat cgacgggatc gatcgtattc
    13681 gctttaccac cagccatccg atcgaattca ccgacgatat catcgaagtg taccgcgaca
    13741 cgcctgagct ggtgagcttc ctgcatctgc cggtacagag cggttccgat cgcattctga
    13801 acctgatggg acgtacccat acggcgctgg agtacaaagc gatcatccgt aaactgcgtg
    13861 cggcgcgtcc ggatattcag atcagttctg acttcattgt tggcttccct ggcgaaacca
    13921 ccgaagactt cgagaaaacg atgaagctga ttgccgacgt caatttcgac atgagctaca
    13981 gctttatctt ctctgcacgt ccaggaacgc ctgccgccga tatggttgat gatgttccgg
    14041 aagaagagaa gaagcagcgt ctgtatattc tgcaagaacg tatcaatcag caagcgatgg
    14101 catggagccg tcggatgctc ggcaccaccc agcgtattct ggtagaaggg acttcgcgta
    14161 agagcatcat ggagctttcc gggcgtaccg aaaataaccg cgtggtcaac ttcgagggca
    14221 caccggatat gatcggtaaa ttcgtcgatg tagaaattac cgacgtctac ccgaactctc
    14281 tgcgcggtaa agtggtgcga actgaagatg aaatgggtct gcgcatggca gaaacaccgg
    14341 agtcggtgat tgcccgcacc cgcaaagaaa acgaccttgg cgtgggttat tatcagccgt
    14401 aattctcagg cctgccgttc cggtgggcct tgttttttct ctctttatcc ccatctttat
    14461 cgcaatgctt gcgccttttt cctgtggcgt gaataatttc cttataggcc ggtgaatgtt
    14521 cagcttccgg cggcatacag ataagaggaa cggtttgaac atagacactc gcgaaatcac
    14581 cctggagcca gcagacaatg cgcgtctgtt gagcctgtgc ggcccgtttg atgacaacat
    14641 caagcagctc gaacgccgtc tcggcatcga gatcaatcgc cgcgataacc actttaaact
    14701 gaccggccgt ccgatttgcg tcaccgctgc ggcagacatt ctgcgtagcc tgtatgtcga
    14761 tactgccccg atgcgcggtc agattcagga tatcgaaccg gaacagatcc accttgcgat
    14821 taaagaagcg cgggtactgg agcaaagcgc ggagagcgtg ccggagtacg gcaaagcggt
    14881 caatatcaaa actaaacgcg gcgtaattaa gccgcgcacg ccaaaccagg cgcagtacat
    14941 cgccaatatt ctcgaccatg acattacctt cggcgttggc ccggcgggta cgggtaaaac
    15001 ctacctggca gtggctgcgg cagttgatgc cctggagcgt caggaaattc gccgtattct
    15061 gctgactcgt ccggcggtcg aagccggtga gaaactgggc ttcctgcctg gcgatttaag
    15121 ccagaaagta gacccgtatc tgcgcccact gtacgacgcg ctgtttgaaa tgctgggctt
    15181 tgagaaagtc gagaaactga ttgagcgcaa cgttattgaa gtcgcgccgc tggcctatat
    15241 gcgtggtcgt acgctgaacg acgcgtttat cattctcgat gagagccaga acactaccat
    15301 cgaacagatg aagatgttcc tgacccgtat cggttttaac tcaaaagcgg ttatcaccgg
    15361 cgacgtcaca cagatcgacc tgccgcgtaa tactaaatca ggcttacgtc atgccattga
    15421 agtgctggcc gatgtcgaag agatcagctt taacttcttc cacagcgaag acgtggttcg
    15481 tcacccggtg gtggcgcgta tcgttaacgc ttatgaagcc tgggaagaag cagaacaaaa
    15541 acgtaaagcg gcgctggcag cagaacgcaa gcgcgaagaa caggaacaaa aatgagtcag
    15601 gtgatcctcg atttacaact ggcatgtgaa gataattccg ggttaccgga agagagccag
    15661 tttcaaacat ggctgaatgc ggtgatcccg cagtttcagg aagagtcgga agtgactatt
    15721 cgcgtggtcg ataccgccga aagccacagt ctgaatctga cctatcgcgg taaggataag
    15781 ccgaccaacg tgctctcctt cccgtttgaa gtgccgcctg gcatggagat gtcgctactg
    15841 ggcgatctgg ttatctgccg tcaggtggtt gagaaggaag ctcaggagca aggcaaacca
    15901 ctggaggcgc actgggcgca tatggtggtg cacggcagtc tgcatttgtt aggttacgat
    15961 cacattgaag atgacgaagc agaagaaatg gaagccctcg aaacagagat tatgcttgct
    16021 ctgggctatg aggatccgta cattgccgag aaagaataat ttgccaggca tttgactggc
    16081 agacgtcccc gccgttggtg attaacgacg gcgttttgat taactaacat gagaacccat
    16141 acgacgccat gagcgacgac aattcacaca gtagtgacac gataagcaac aagaagggat
    16201 ttttctccct gttactcagc caacttttcc acggtgaacc gaaaaaccgt gacgaactgc
    16261 tggcgctgat ccgtgattcc gggcagaacg accttatcga cgaagatacg cgcgatatgc
    16321 tcgaaggggt gatggacatc gcagaccaac gcgttcgcga catcatgatc ccccgctccc
    16381 agatgattac cctgaaacgc aaccagacgc tggacgaatg ccttgatgtc atcatcgagt
    16441 ccgcccactc acgtttcccg gtgattagcg aagacaaaga tcacattgaa gggattctga
    16501 tggcgaaaga cttgctgccg tttatgcgca gcgatgctga agccttcagc atggacaaag
    16561 tgttacgtca ggcggttgtc gttcctgaaa gtaagcgcgt agaccggatg ctgaaagagt
    16621 ttcgctctca gcgttaccac atggcgatcg ttattgacga attcggtggg gtttccggtc
    16681 tggtgaccat tgaagacatc ctggaactga ttgttggtga gattgaagac gagtatgacg
    16741 aagaagatga tatcgacttc cgtcagctga gtcgtcatac ctggaccgtg cgcgcactgg
    16801 cttccattga agacttcaac gaagcgttcg gcacccactt tagcgatgaa gaagtcgaca
    16861 ctatcggtgg tctggtgatg caggcgtttg ggcatcttcc ggcgcgtggc gaaactatcg
    16921 acatcgacgg ttaccagttc aaagtggcga tggccgatag tcggcgtatt attcaggttc
    16981 atgtcaaaat cccggatgac tcaccccagc caaagctgga tgaataaaac cgaaactgga
    17041 tagataacta catggatttt gcctcattaa ttgaacgcca gcgcattcgc ctgctgctgg
    17101 cgttattatt cggtgcctgc ggaacgctgg ccttctctcc ttacgacgtc tggcctgcgg
    17161 cgattatttc gctgatgggg cttcaggcgt tgacctttaa ccgtcgccca ctccagtctg
    17221 ccgctattgg cttttgctgg ggatttggcc tctttggcag cggtattaac tgggtctatg
    17281 tcagcatcgc gacctttggc gggatgcctg gcccggttaa catcttcctg gtggtgctgc
    17341 tggcggcgta tttgtcgctg tataccggac tgtttgctgg cgtgctgtcg cgtctgtggc
    17401 cgaaaaccac ctggctgcgc gtggcgattg ccacccctgc tctctggcaa gtgaccgagt
    17461 ttctgcgcgg ttgggtgctg actggcttcc cgtggttaca gttcggctat agccagattg
    17521 atggcccgtt aaaagggctg gcaccgctaa tgggcgtgga agccattaac ttcctgctga
    17581 tgatggttag tggcctgctg gcactggcgt tggtcaaacg caactggcgt ccgctggtgg
    17641 tggccgtcgt gctgtttgcc ctacccttcc cgctgcgtta catccaatgg tttaccccgc
    17701 aaccggagaa aaccattcag gtttcgatgg ttcagggcga tattccgcaa tcgctgaaat
    17761 gggacgaagg ccagcttctt aatacgctaa agatttacta caacgcaacg gcaccgctga
    17821 tgggcaaatc atcgttgatt atctggccgg agtcggcgat aaccgatctg gaaattaatc
    17881 agcaaccgtt cctcaaagca ctggacggtg agttgcgtga taaaggtagc tcgctggtaa
    17941 ccgggattgt cgacgcgcgt ctcaataagc agaaccgcta cgatacctac aacaccatca
    18001 tcacgctggg taaaggtgca ccgtacagct acgaatcagc cgatcgctat aacaaaaacc
    18061 atctggtgcc gtttggcgag tttgtcccgc tggagtcgat tctgcgtccg ttagcaccgt
    18121 tctttgatct gccgatgtcg tcgttcagcc gtgggccata tatccagccg ccgctgtcgg
    18181 caaatggtat tgagcttact gcggctattt gctacgagat tattctcgcc gagcaagtgc
    18241 gcgataactt ccgcccggat accgactacc tgctgactat ctccaacgat gcgtggtttg
    18301 gtaaatctat tggtccatgg caacacttcc agatggcgcg aatgcgtgcg ctggagctgg
    18361 cgcgcccact gttgcgcagc accaacaacg gcattacggc ggtgattggc ccgcagggtg
    18421 agattcaggc gatgatcccg cagttcgccc gcgaggtgtt aaccactaac gtgacgccga
    18481 ccaccggact cacaccatac gcacgtaccg gcaactggcc gctgtgggtg ctgacggcac
    18541 tgtttggttt tgctgctgtg ttgatgagtc tgcgtcagcg acgtaaataa tcccttctta
    18601 tcgtgccgga atacatccca tatttcggca cgctcaccgg atgcggcatc agtgcattat
    18661 ccggataaca atattctcct atccaacttt ggcacatcta ttgctttgtt atacaaggca
    18721 aagcttgaac cagcatcagt acaacccggt tgcaccacag caatccaccg gtagcaccaa
    18781 aagagtgcat tttaagaatt ttgcttcaga atggtgcggt gcacctcgca aaaacaaaca
    18841 ataaaacatc aatatcttta catttagcca aactaaatgt tacctaacag gcacaacact
    18901 gcacaataaa gttgcagacg ataacaacac aaacactcac aacgggtatc catgcgttct
    18961 taacgcagaa gataaaggtg ttggatatgc aattacgtaa acctgccaca gcaatcctcg
    19021 ccctggcgct ttccgcagga ctggcacagg cagatgacgc cgccccggca gcaggcagta
    19081 cgctggacaa aatcgccaaa aacggtgtga ttgtcgtcgg tcaccgtgaa tcttcagtac
    19141 ctttctctta ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat tactccaacg
    19201 ccattgttga agcagtgaaa aagaaactca acaaaccgga cttgcaggta aaactgattc
    19261 cgattacctc acaaaatcgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg
    19321 gttctaccac caacaacgtc gaacgccaaa aacaggcggc tttctctgac actattttcg
    19381 tggtcggtac gcgcctgttg accaaaaagg gcggcgatat caaagatttt gccgacctga
    19441 aaggcaaagc cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga
    19501 atgaagagca aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc
    19561 gcaccctgga aagcggtcgt gccgttgcct ttatgatgga tgacgctctg ctggccggtg
    19621 aacgtgcgaa agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg
    19681 aagcctacgg ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg atggatgaca
    19741 ctatcgctca ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa
    19801 atccaattcc gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac
    19861 tgttcaaaga accgaatgac aaggcactga actaattaca agaaccaggg gcggaaattc
    19921 cagccctctc gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc
    19981 gggcctgaaa accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta
    20041 ccctcttttt tctggagtag atttatgtct atagactgga actggggtat ttttttacaa
    20101 caagccccgt tcggcaatac cacctatctt ggttgggtct ggagtggttt tcaggtgacg
    20161 atcgctttgt cgatctgcgc ctggattatc gctttcctcg tcggttcatt tttcggcatt
    20221 ttacgtaccg ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc
    20281 cgcaacgtgc cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagctgctg
    20341 ccggagaaaa tcggcatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt
    20401 tcatccatgc tctgcctggg gctgtttacc gccgcccgtg tttgcgaaca ggtacgcgcc
    20461 gctattcagt cgctaccgcg tgggcagaag aatgccgcgc tggcgatggg cctgacgcta
    20521 ccgcagactt atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg
    20581 acttccgaga tgatgaacct ggtgaaaaac tccgccatcg cctcgaccat cggtctggtg
    20641 gatatggcgg cgcaggcggg taaattgctc gactactcgg ctcacgcctg ggagtcgttt
    20701 accgccatca cgcttgcgta tgtgttgatt aacgctttta tcatgctggt gatgacgctg
    20761 gttgaacgta aagttcgcct gcccggcaac atggggggca aataatgtac gagtttgact
    20821 ggagttccat tgttccttcc ctgccatatc tgctcgacgg gctggtgatc accctgaaaa
    20881 tcaccgtcac ggcagtcgtg attggtattt tgtggggtac catgctggcg gtgatgcgct
    20941 tgtccagctt cgcgcctgtt tcgtggtttg ccaaagccta cgttaacgta ttccgctcga
    21001 ttcctttagt catggttttg ctgtggtttt acttgattgt gccgggattt ctgcaaaatg
    21061 tgctgggatt atcgccaaaa aatgatattc gtttaatttc ggcgatggtg gcgttttcga
    21121 tgtttgaagc ggcctactat tcagagatta tccgtgccgg tattcaaagt atttcccgtg
    21181 gtcagtcgag cgccgcgctg gcgttgggga tgactcactg gcagtcgatg aaactgatta
    21241 ttctgccgca ggctttccgc gcgatggtgc cgctgctgct cactcagggc atcgtactgt
    21301 tccaggatac ctcgctggtg tatgtgttat ctctggccga tttcttccgt accgcctcaa
    21361 ccattggtga gcgtgatggt acgcaggtcg agatgatcct gtttgccgga tttgtttatt
    21421 tcgttattag ccttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat
    21481 gattaccctg aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc
    21541 aaccgaagtg aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc
    21601 aacgctgatt aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga
    21661 tggtatcgtg gttaacgaca agaaaaccga tctggcaaag ctgcgctccc gcgtcgggat
    21721 ggtattccag catttcgagc tgttccctca tctgtcgatt atcgaaaacc tgaccctggc
    21781 gcaggtgaaa gtgcttaaac gcgataaagc gcccgctcgt gaaaaagcat tgaaattgct
    21841 ggagcgcgtg ggactttccg cacatgccaa taaatttccg gcgcagcttt ccggcggtca
    21901 gcagcagcgc gtggcaatcg ctcgcgcatt gtgtatggct cctattgcga tgctgttcga
    21961 cgaaccgaca tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga
    22021 actggcgaac gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa
    22081 agtggcgaat cgggtgatct ttatggacga gggtaaaatt gtcgaagact cgccgaaaga
    22141 cgctttcttc gatgatccga aatcggaccg cgcaaaagac ttcctcgcga aaatcctgca
    22201 ttaatcactc tggcgcacat acccaattgc gcgccatctc acgctttatc gtttcgcccc
    22261 tcgtatctgt tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac
    22321 aatggcactg ccaattctgt tagattgcga cccaggtcat gatgacgcta tcgcaatagt
    22381 tctcgcactc gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca
    22441 gacaccagaa aaaaccttac gcaatgttct gcgtatgctg accttgctta atcgcaccga
    22501 tattccggtg gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa
    22561 tgtgcacggc gaaagcggtc tcgacggccc ggcattaccg gaaccgacgt tcgcaccgca
    22621 aaactgtacg gcggtggagc tgatggcgaa aacgctgcgc gaaagtgcgg aacctgtcac
    22681 gattgtttct accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact
    22741 gcatagcaaa attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac
    22801 gcctgcggct gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc
    22861 agggatcccg gtggtgatgg ccggtctgga tgttactcat aaagcgcaaa tccacgttga
    22921 agacaccgag cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct
    22981 ggatttcttc ctcgaatatc ataaagacga aaaatggggc tttgtcggcg caccgctgca
    23041 tgacccatgc accatcgcct ggctgttgaa acccgagttg tttaccactg ttgagcgctg
    23101 ggttggcgtg gaaacacagg ggaaatatac ccagggtatg acggttgttg attattatta
    23161 tctgactggc aataaaccga atgccactgt aattgtcgat gttgatcgtc agggctttgt
    23221 tgatttactg gctgatcgtc tgaaatttta cgcttaacac tcgtcattga ttaagatcac
    23281 cacgcgttac ctgggtggtc ttaatctatt tatctctttt tctttcaaga tataaatgca
    23341 atggataatg cagaactcgc cataggtatc gatctcggca ctaccaatag tttaattgcc
    23401 gtctggaaag acggtgccgc gcaattaatt ccaaataagt tcggtgaata tttaacacca
    23461 tccataatta gcatggatga aaataatcat attttagtcg gaaaaccggc tgtatcacgg
    23521 cgtacttcgc atccggataa aacggcagcg ttatttaaac gtgcaatggg cagtaatacc
    23581 aactggcggt taggcagcga cacatttaac gcgccagaac tgtcctcttt ggtattacgc
    23641 tcattaaaag aagatgccga agaatttctg caacgtccga ttaaagatgt ggtgatctcc
    23701 gttctggctt atttcagcga tgaacaacgc aagcataccc gtttagcagc ggacttagcc
    23761 gggttaaatg cggtacgctt aattaatgaa cccacagcag ctgcgatggc gtatggcctg
    23821 catacccaac aaaatacccg ttcgctggtg tttgatctcg gtggcggcac gtttgacgtt
    23881 acggtgcttg agtacgccac gccggtgatt gaagttcacg cctccgctgg cgacaacttc
    23941 ctcggtggcg aagattttac ccatatgttg gtcgatgacg ttttaaagcg cgcggatgtc
    24001 acaaggacca cgcttaacga gagtgaactg gcagccttgt acgcctgtgt ggaagcggca
    24061 aaatgtagca atcaatcgcc aatgcacatt cgctggtagt accaggatga aacgcgggaa
    24121 tgcgaatttt acgagaacga actggaagat ttgtggttgc cgctgctcaa tcgcttgcga
    24181 gtgccgattg aacaggcgtt gcgcgatgcg cgtctgaagc cgagtcaaat cgacagtctg
    24241 gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt gcgtctgttt
    24301 ggcaaattac cgtatcaaag ttacgatccg agcaccattg tcgcgctggg cgcagcaatc
    24361 caggccgcct gccgcttacg cagtgaagat attgaagaag tgatcctcac tgatatttgc
    24421 ccttactcgt tgggcgttga ggttaaccgc cagggcgttt ccggcatttt ctcgccgatt
    24481 attgaacgaa acaccactgt gcccgtgtcg cgtgtagaaa cttattcaac catgcacccg
    24541 gaacaggatt caattacggt taacgtctat cagggagaaa accacaaagt taaaaacaac
    24601 attctggtgg aatccttcga tgtgccgttg aagaaaaccg gggcttatca gtcgattgat
    24661 attcgcttta gttatgatat caacgggttg cttgaagttg acgtgcttct ggaagacggc
    24721 agcgttaagt ccagagtgat taaccacagc ccggtaacat tgagcgcgca gcagattgaa
    24781 gagagtcgga cgcggttatc cgcattgaaa atttatccgc gcgatatgct catcaatcgc
    24841 acctttaaag ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga gcgagaagag
    24901 attggccggg tgatcaccga ttttgatgcg gcgctgcagt caaacgatat ggcccgcgtc
    24961 gatgaggtca ggcggcgggc gagcgtttat ttagccattg agacctcata atcacggctg
    25021 ccggatgcgg cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg tcaactaaac
    25081 aacatcgcga ttttgccgag agcataacct ataaccagca ccgttaaact gattttgata
    25141 atgctggtaa tgctctgtgg tttttcaccc tcatcatttt tttcttcatg gttttcaggt
    25201 gttggctcat cgacatcttc atcctccggc tcgagtttta ctttcgtcaa cagcatccgg
    25261 gtgataatgg cctctaatgg atgagcatct ccgccttcca gccttgtatt aagacgggcc
    25321 agcatctctt gtttcggaaa cgcttcaagt actacctgtt ccgctacccg atacaaatcc
    25381 agtaacggtg aatcttcttc tgtctcgcta ccgactctcg atttccacca cgcccatgct
    25441 ttgcgtttga agaataacga ctcaataaac gattgaccta acggcgataa ttttccgcta
    25501 tgtaaaattt ccgcccataa cagccgcgtc cgggcgctct caccaggaac atatttcagc
    25561 ggttgacctt gcgcatctac catgctccgc gcttcgataa ccatcactaa taacgcgaaa
    25621 taatccgggc aatgacgctt gctccactgc aacaacaaat tatcagcttg tgtagatggg
    25681 taactttccc agtacgcgcc gagatcagca agcaggcttt cctcctcgcc gcagtacaaa
    25741 cgttgtgcac actggtaata ccgggcatct tcgctttctg gttcttccgc ttgccactgt
    25801 ttggctacag gaatgagttc caccatacct gtttgcaccg agctgtacca gcgcaacagt
    25861 ttgcgatgta actttgcatc atcaggaatg aaccacggtc cttccatcgc caaaaacgca
    25921 gtgacatagt caggatggta acggcagcaa cgttccagcg cgaagtaata ttcaacagtc
    25981 tggttctgta acgcgacggg atattccaga aggctaagga aatcgaaaca atcaccagcc
    26041 tgaatggcct caagaaaagc ctccctttct tcctcatcca ttccttcgct gtcattaaac
    26101 gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat ttaatgacag atatcgcgct
    26161 tctatagcga ttgcacacag cggccagcgt aattgatcga catcctccat gttccaggta
    26221 tttaattgct gaataaattt ctgccaggcg gagggctgaa aacgatcact ttctgaatcc
    26281 agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg tggcatcttt ttcttcatca
    26341 tctgcttcct cgacaggatt taccgccagt cgcagcgcct cttcataggc ctggcgtaat
    26401 gctttaaacc cctgcggatc ggtttcggga tgacacaacg gtaagcgagc aagataagcc
    26461 tggcggataa tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca acatgttttc
    26521 ataggtcgct cacatcctta agcgttaccg ggcattcagg ctggacagtt aatggctgcc
    26581 agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag ctcttcgaac atccgactcc
    26641 cggcgctatc gtatttgtac agggttagcg cctcgcgtag cgaagcgttg tcttcgcact
    26701 gagaagattg ccagtagaga cgccctagtg aataggcaga aaaatagtgc atccagctgt
    26761 gatagtaatg gtgagctcgt gcatagacgc gcgattgcag ccataaactc tcttcttccg
    26821 tcagccagtt gttgagcaca ccggcccgtg acagaaatcc cattcgaaca taatcccagg
    26881 ctttgattcc cccttctcca cagcacatag cggtttgggc aacgaattgg gtataaatca
    26941 accctctctc cgagccacca gcaattaatg ctttccactc ttcaggggaa gagcgttgcc
    27001 atgtcagata taagccagct aaatcattgg catgaccatc atcagtcatc tgataaatag
    27061 tctgaattaa atcatcacga ttatttattc cccaggatga tgagagatcg aattctttta
    27121 tcccggtata catttttggc gagtcatgcg cgggtttgca actctgatta agtatttcca
    27181 tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt attcattttt taataatccc
    27241 ttaagtgcat taactgacta cttttttatt attgatgagt ataaacttga cgctttcaaa
    27301 ataaaaagaa aatcgaagca ttcacacatg aataaaaaat taatgtatat attcgcaatt
    27361 tttatagttg cagcaattac ctgtattagc caacccaaga aaacgatgtt gcgtgataaa
    27421 gccatggtga attatgcctt tgattattta agatcaccag gcagtctacc gttaaccacg
    27481 gcagccacgg agctttccgc gattcatggt cactcaacat cgcaatatcg ccttggcgaa
    27541 ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat
    27601 gagcaatcag cagaacagga aaatccgcgc gcacaaagca aactggggtg gatctacctg
    27661 aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc tctggtataa ggaagcagct
    27721 gaacaagggt atgctcatgc tcaatatact ttgggtttga tctacagaaa tggcactggt
    27781 attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac
    27841 aaaaatgcgg aaagattact tgccggactt cccgcacatt aacgtcgact gtcacccgaa
    27901 gagatggagt attttcccta tcaaaccggc aaagataaaa atgtagaaaa taattttgat
    27961 gacgcccaga cagcccagcc cgccatcatc aacgggcatt ggctgcggtt gcggctccgg
    28021 ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt aataatcgac gaataattaa
    28081 tgcttccagt ggacaacgat cttctgcatc aaggcggctc acaatcggca ttaatgattc
    28141 ttctgtcggt tggctggcct gcactaattg tttggcaact aaatataatg gccataaggg
    28201 atgttctgta tcacgcgtga gtgagatccg cggcttacgt cctttgtgca aaaccatctc
    28261 gaccagtgaa cgccccagcg cagagagttt ttcatcgctg agtatttcgt ataaatgaaa
    28321 acgcgtacgg gcgctgtcgc cggggacata aagtagcggt tttccctgat cgttaaccag
    28381 atcacgcgct tcaatcatca tcaccactaa tgggtaataa tagacccgat gctgacggca
    28441 ccattgcaac attaaagcat ccgcctgggt ggagggatac tcgcgccagt agtaacaaag
    28501 ttctgccagc aggctttcgc cttctccaca atagacgcgt tgtgcgtatt cataataacg
    28561 cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg
    28621 gattcccagg cggcttgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc
    28681 ggggataatc actgcgcgat gttgcgctaa atacaatgtc agccattctg gatgcgatga
    28741 ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caactggcag
    28801 atgcagaatg gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc
    28861 cagttcttct tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca atctttctgc
    28921 taataacacc acgcactcga aggataaatg ggcagtgttc atggcgattg tgcacagcga
    28981 ccagcgtaat tcatcaatct cttccatcga gcaataattt aattgctgaa tgaatcgctg
    29041 ccaggcggag ggcagaaaac gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag
    29101 aatttcatgt tctgctacct catattcttc tggttgccaa acagatttag caggcgactg
    29161 cgcaatccgt agcgcctcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt
    29221 ttctggatga aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt
    29281 cgtttcctct atatcgagga tcttccagca atttttcata agtcgctcat atccttaagc
    29341 gtttccgggc atgtggggta gtcagccagt ggttgccagg acaaggtagg gtaatacgca
    29401 tcatcactgg caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc
    29461 gctaagaatt cgcgtaataa aggcagatga tcatcttcgg gagactgcca atattgccga
    29521 ccaaaggtat aaccggcaaa gtattgccgc cagttactat aataacgtag agcccgtaga
    29581 tgaatacgcg actggatcca tagactttct tcttcactca accagttatt aagtacgccc
    29641 attcgactta aaaaacccat ccggacataa tcccatgctt taattcctcc ctcaccacag
    29701 cattcggctg tgctggcgac aaattgcgca taagcctgac cttgctcatt taattcagca
    29761 agatagtcac gccattcaca tggagaataa cgaaaccagc ggtgataaaa ccctgccaac
    29821 cgagcggcat gaccgttctc gatcattcgg tgaattatgc gcagcaggtc atcacggtca
    29881 tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggggat
    29941 gaatgcgaag gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat
    30001 attttttgcg accccatgtc catataattt atccttaaac attcatttaa tgaatgtaaa
    30061 ctacatttcg aggctcagtt atttatactt ttctgacgca acataatacg cataaaataa
    30121 ggcttacgca aaaatggaac acataaaaat ataatatatt ttatctacaa ccataaggaa
    30181 atgataatga tttttacgtc aagttgctgc gataatttat caatagatga gattatcgaa
    30241 cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat
    30301 agcgcaattg actctccgga cgacgaaaag gccttttact ggctgaagct ggccgctgag
    30361 caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt
    30421 cataaagata atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact
    30481 ttcgcttcca acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa
    30541 gcggttacct ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt
    30601 ggatggatgt acagaaatgg caacggagtc gcacaagact atacgctggc atttttctgg
    30661 tacaaacaag ctgcattaca aggccatagt tacgcacaag acaatctggc cgatctttat
    30721 aaagacggag aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc
    30781 gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa
    30841 ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa
    30901 agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa
    30961 gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc
    31021 tggtataacc tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag
    31081 gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt
    31141 cgtgagactg aagctttact gtagaaataa aaacaagggc cggggaattt cattctgccc
    31201 atattctctt tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa
    31261 ctggtcataa ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg
    31321 gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac
    31381 cccatgatga ttgaggtcct ggaaaatttc gcgccattca agctgcgttt tatcggtgat
    31441 gtctgccagc tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc
    31501 ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg
    31561 cgtcagctca tcgatctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc
    31621 ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgt tcagtgacgc
    31681 gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac
    31741 tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta
    31801 aattaatgta cgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg
    31861 acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta
    31921 gccgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata
    31981 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
    32041 agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg
    32101 ggccacgtac gtaactacac catcggtgac gtgatcgccc gttaccagcg catgctgggc
    32161 aaaaacgttc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
    32221 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
    32281 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
    32341 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
    32401 aaaaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa
    32461 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag
    32521 tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat
    32581 cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg
    32641 gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
    32701 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
    32761 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
    32821 gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagctgtt
    32881 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
    32941 ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct
    33001 aaatacggcc tgaacatcaa accggttatc ctggcggctg acggctctga gccagatctt
    33061 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt
    33121 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
    33181 cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gccagcgtta ctggggcgcg
    33241 ccgattccga tggtgacgct ggaagacggt accgtaatgc caaccccgga cgaccagctg
    33301 ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat
    33361 ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc
    33421 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa
    33481 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt
    33541 atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat
    33601 gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg
    33661 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
    33721 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
    33781 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg
    33841 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
    33901 ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg
    33961 aaacgtgtct ggaaactggt gtacgagcac acagcaaaag gtgatgttgc ggcactgaac
    34021 gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct
    34081 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
    34141 gagctgatga acaaactggc gaaagcaccg accgatggcg agcaggatcg cgctctgatg
    34201 caggaagcac tgcaggccgt tgtccgtatg ctcaacccgt tcaccccgca catctgcttc
    34261 acgctgtggc aggaactcaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
    34321 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
    34381 cgtgccaaaa tcaccgtccc ggtggacgca acggaagaac aggttcgcga acgtgctggc
    34441 caggaacatc tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta
    34501 ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg
    34561 caacactgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg
    34621 acaccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg
    34681 ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata
    34741 aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag
    34801 ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga
    34861 atgcgaccgt gttgatcccc ggccgtgata tctacccgat tagcgccaaa gtcttccgtt
    34921 cattcttcga taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa
    34981 aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg
    35041 cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg
    35101 cacgcgtctc caccatgctg ggtaactgat gattcggttg tacccggaac aactccgcgc
    35161 gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt
    35221 gcaggaaagc caggacgctg ttcgccaggt agctgcggca caaggattcg aagaacacca
    35281 cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat
    35341 gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc
    35401 ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc tgctattgat
    35461 cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc
    35521 gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt
    35581 tgctgtgcgc gcaaaacagc tcaacttaga actggatgac gcggcgaatc aggtgctctg
    35641 ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct
    35701 ctggccagac ggcaaactga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca
    35761 tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca
    35821 tattcttcag caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca
    35881 acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc
    35941 gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa
    36001 tcgcttaagt cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac
    36061 cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt
    36121 gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc
    36181 tgtttggcgg cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg
    36241 cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc
    36301 cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca
    36361 agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc
    36421 aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg
    36481 gtcaggattc attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg
    36541 cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc
    36601 agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg
    36661 gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac
    36721 gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtattg acttacatta
    36781 accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt
    36841 aattcgtcat ggcgaaacac aagcgaatgt cgatggtctg tacagcggtc atgcgcccac
    36901 acccctgacc gcacgcggta ttgagcaagc gcaaaatctg catacactgc tagatgatgt
    36961 ttcctttgac ttggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt
    37021 tctcagtgac cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg
    37081 cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc
    37141 gtggtgcaat gactggcagc atgctatccc cacgaacggt gaaggatttc aggcattttc
    37201 gcaacgtgtg gaacgcttta tcgcaaggct tagtgaatat cagcactatc agaatatttt
    37261 aatcgtcagc catcagggcg tactgagtct gttaattgct cgtttaattg gcatgcctgc
    37321 cgagtctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg atatcaacca
    37381 aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga acgcatgact
    37441 tttctgtttt tttacgggta agccgcaacg accattgaca gccccgggca ggctgatatt
    37501 ctccgcagcc agactttttc cgccagacac gactttgtag aaattgtttt acaaaaatgg
    37561 cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac
    37621 aattgtcccg aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat
    37681 tcacccaggg ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg
    37741 atgacctcaa aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg
    37801 actgcatgat catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg
    37861 ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg
    37921 ccgactggat tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc
    37981 gtcgcctgta tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg
    38041 tgggaacgaa aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc
    38101 cgaaagatat gcccttcgag ctgattgaaa ttccggccgg caaacgcggc aagaatgcgg
    38161 acatcaagcg catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc
    38221 gcattgtcac cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc
    38281 tggaacgctg gaagctggat ggtcgcgacg taagtctact gattggcggg cctgaagggt
    38341 tgtcgcctgc ctgtaaagcg gcggctgagc agagctggtc gctgtcggcg cttaccctcc
    38401 cccatccgct ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca
    38461 ccaaccatcc ttatcaccgt gagtgataag ggagctttga gtagaaagcg cagcggatga
    38521 aactacagaa ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc
    38581 tggtcgcctt tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc
    38641 tgcaaattgt tcgctttacc gactaccaga cccgctctaa tgaaaaccgc attaagctgg
    38701 tgcctatcgc gcccagccgc ggcattatct acgaccgtaa cggtatccct ctggccctca
    38761 accgcactat ctaccagata gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc
    38821 tggacgcttt gcgcagcgtg gtagatctga ccgatgacga tattgctgca ttccgaaaag
    38881 agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa aactaaccta accgaagtac
    38941 aagtagctcg ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata
    39001 aacgtcgtta ctatccttac ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa
    39061 tcaacgataa agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa
    39121 cgcatgatat tggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga
    39181 ccggttatga agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag
    39241 taccaccgca agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata
    39301 ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt taccgaaccg cgtacaggtg
    39361 gggtgctggc gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct
    39421 ccagcaaaga ttattccgcc ttgctgaacg acccgaatac accgctggtg aaccgcgcca
    39481 cacagggggt ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattaa
    39541 gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactaccag
    39601 ggtcggaaaa acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa
    39661 gatcgctgga agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg
    39721 atcgcctctc cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg
    39781 cggaagaacg ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac
    39841 cgtggtatca gggtgacacc attccggttg gtatcggcca gggttactgg acagcgaccc
    39901 caatccagat gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttccgc
    39961 atttgctgat gagcaccgcc gaagacggca aacaggtgcc atgggtacaa ccgcatgaac
    40021 cgcccgtcgg cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg
    40081 ttgctaaccg ccctaatggt acggcgaata aatactttgc cagcgcaccg tacaaaattg
    40141 cggcgaaatc tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc
    40201 acaaaattgc cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca
    40261 atccgcaagt ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg gcggttggta
    40321 cgctgatgcg ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg
    40381 cggaaaatcc agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa
    40441 aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgattt tactggcatt
    40501 gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga
    40561 gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc
    40621 tccacgcgtt tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt
    40681 ggcggtagat gctttcggag ccatctctaa aggcgctcaa cgctggctgg acctcggtat
    40741 tgttcgtttt cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt
    40801 tatcaaccgc gacgtttgcc cgccatcgtt gaagaatact ggcatcgcgc tggtgctgat
    40861 atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc
    40921 gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc
    40981 agtagtgctg gtagcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca
    41041 gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca
    41101 cattattcag tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca
    41161 cggcactcag tcacagcttg aatttctccc cgaacgccat actgacttta tcttcgcggt
    41221 actggcggaa gagctgggat tagtgggcat tctgattctg ctcgctctct acattctgct
    41281 gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc
    41341 tggtggctta atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg
    41401 tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat
    41461 tgtgctgatg gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc
    41521 gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag
    41581 gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc
    41641 ctgcggtatg taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac
    41701 tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg
    41761 atccgtctcg atttagccag gcgggactgg cggcaatcta tgatgccgaa ccaggcagta
    41821 acctgacggc ctctggcgaa gctttcgatc cgacaaagct gacggcggcc catccaacgc
    41881 ttccgatccc cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc
    41941 gcattaatga tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag
    42001 ctgaccgtct taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc
    42061 aggatggttc gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttacg
    42121 ccctgcctgc acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc
    42181 agggtgacat tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc
    42241 cggtaaccag tagcggtttc ctcggcgctc caacgacctt agcgcctggt gtactggaag
    42301 gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc ttcgtcgaca acgcctgcaa
    42361 cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg
    42421 tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac
    42481 agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg
    42541 gcccattcgc cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc
    42601 aattacagtc atttattact accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt
    42661 tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg
    42721 ctatagtagg gcactttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    42781 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    42841 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    42901 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    42961 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    43021 gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    43081 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    43141 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    43201 gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    43261 gctacgttaa cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg
    43321 ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg
    43381 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    43441 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    43501 gacacactga caaagcaggt tacaatcttg ttgcttctgc gactgaaggc cagatgcgct
    43561 tgatttctgc ggtgatgggt ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    43621 tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    43681 tcgcctctga accggtttgg tttagtgatt ctgatcgcgc ttcgttaggg gttgataaag
    43741 acgtgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    43801 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    43861 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    43921 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    43981 aaaaattgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    44041 ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    44101 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    44161 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    44221 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    44281 cgccactcat atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt
    44341 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct
    44401 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    44461 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccggg
    44521 ctatgcatga attcaccgat acccgcgata atagtaccct tgatgaaatc tggctggtcg
    44581 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    44641 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    44701 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    44761 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    44821 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    44881 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    44941 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    45001 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    45061 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    45121 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    45181 attttcttta tcacttgcag aaatgcggtg ccacgccttg ctccgtaact caccccattg
    45241 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    45301 agattattgt aatt
//
LOCUS       DAZOZX010000019        44705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZOZX010000019 DAZOZX010000000
VERSION     DAZOZX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.019"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(17..904)
                     /gene="rpnA"
                     /locus_tag="ACHUPT_001011"
     CDS             complement(17..904)
                     /gene="rpnA"
                     /locus_tag="ACHUPT_001011"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR0818680.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
     gene            complement(1107..1343)
                     /gene="feoC"
                     /locus_tag="ACHUPT_001012"
     CDS             complement(1107..1343)
                     /gene="feoC"
                     /locus_tag="ACHUPT_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0818681.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1343..3664)
                     /gene="feoB"
                     /locus_tag="ACHUPT_001013"
     CDS             complement(1343..3664)
                     /gene="feoB"
                     /locus_tag="ACHUPT_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0818682.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3681..3908)
                     /gene="feoA"
                     /locus_tag="ACHUPT_001014"
     CDS             complement(3681..3908)
                     /gene="feoA"
                     /locus_tag="ACHUPT_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0818683.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4346..6667)
                     /locus_tag="ACHUPT_001015"
     CDS             complement(4346..6667)
                     /locus_tag="ACHUPT_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0818684.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6764..7240)
                     /gene="greB"
                     /locus_tag="ACHUPT_001016"
     CDS             complement(6764..7240)
                     /gene="greB"
                     /locus_tag="ACHUPT_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0818685.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7468..8187
                     /gene="ompR"
                     /locus_tag="ACHUPT_001017"
     CDS             7468..8187
                     /gene="ompR"
                     /locus_tag="ACHUPT_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0818686.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8184..9536
                     /gene="envZ"
                     /locus_tag="ACHUPT_001018"
     CDS             8184..9536
                     /gene="envZ"
                     /locus_tag="ACHUPT_001018"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0818687.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9612..11234)
                     /gene="pckA"
                     /locus_tag="ACHUPT_001019"
     CDS             complement(9612..11234)
                     /gene="pckA"
                     /locus_tag="ACHUPT_001019"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0818688.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11613..13371
                     /locus_tag="ACHUPT_001020"
                     /pseudo
     CDS             11613..13371
                     /locus_tag="ACHUPT_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13515..14384)
                     /gene="hslO"
                     /locus_tag="ACHUPT_001021"
     CDS             complement(13515..14384)
                     /gene="hslO"
                     /locus_tag="ACHUPT_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0818689.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14409..14810)
                     /gene="hslR"
                     /locus_tag="ACHUPT_001022"
     CDS             complement(14409..14810)
                     /gene="hslR"
                     /locus_tag="ACHUPT_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0818690.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14821..15489)
                     /gene="yrfG"
                     /locus_tag="ACHUPT_001023"
     CDS             complement(14821..15489)
                     /gene="yrfG"
                     /locus_tag="ACHUPT_001023"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0818691.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15554..17689)
                     /gene="igaA"
                     /locus_tag="ACHUPT_001024"
     CDS             complement(15554..17689)
                     /gene="igaA"
                     /locus_tag="ACHUPT_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0818692.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            18009..18569
                     /gene="nudE"
                     /locus_tag="ACHUPT_001025"
     CDS             18009..18569
                     /gene="nudE"
                     /locus_tag="ACHUPT_001025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0818693.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18734..21286)
                     /gene="mrcA"
                     /locus_tag="ACHUPT_001026"
     CDS             complement(18734..21286)
                     /gene="mrcA"
                     /locus_tag="ACHUPT_001026"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0818694.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21406..22185
                     /gene="hofM"
                     /locus_tag="ACHUPT_001027"
     CDS             21406..22185
                     /gene="hofM"
                     /locus_tag="ACHUPT_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0818695.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22185..22724
                     /gene="hofN"
                     /locus_tag="ACHUPT_001028"
     CDS             22185..22724
                     /gene="hofN"
                     /locus_tag="ACHUPT_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR0818696.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22708..23148
                     /gene="hofO"
                     /locus_tag="ACHUPT_001029"
     CDS             22708..23148
                     /gene="hofO"
                     /locus_tag="ACHUPT_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0818697.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23138..23544
                     /gene="hofP"
                     /locus_tag="ACHUPT_001030"
                     /pseudo
     CDS             23138..23544
                     /gene="hofP"
                     /locus_tag="ACHUPT_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23456..24694
                     /gene="hofQ"
                     /locus_tag="ACHUPT_001031"
     CDS             23456..24694
                     /gene="hofQ"
                     /locus_tag="ACHUPT_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0818698.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVMPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25095..25616
                     /gene="aroK"
                     /locus_tag="ACHUPT_001032"
     CDS             25095..25616
                     /gene="aroK"
                     /locus_tag="ACHUPT_001032"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0818699.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25673..26761
                     /gene="aroB"
                     /locus_tag="ACHUPT_001033"
     CDS             25673..26761
                     /gene="aroB"
                     /locus_tag="ACHUPT_001033"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0818700.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26853..28145
                     /gene="damX"
                     /locus_tag="ACHUPT_001034"
     CDS             26853..28145
                     /gene="damX"
                     /locus_tag="ACHUPT_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0818701.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28252..29088
                     /gene="dam"
                     /locus_tag="ACHUPT_001035"
     CDS             28252..29088
                     /gene="dam"
                     /locus_tag="ACHUPT_001035"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0818702.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29106..29783
                     /gene="rpe"
                     /locus_tag="ACHUPT_001036"
     CDS             29106..29783
                     /gene="rpe"
                     /locus_tag="ACHUPT_001036"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0818703.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29776..30534
                     /gene="gph"
                     /locus_tag="ACHUPT_001037"
     CDS             29776..30534
                     /gene="gph"
                     /locus_tag="ACHUPT_001037"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0818704.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30527..31531
                     /gene="trpS"
                     /locus_tag="ACHUPT_001038"
     CDS             30527..31531
                     /gene="trpS"
                     /locus_tag="ACHUPT_001038"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0818705.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31801..32706
                     /gene="yhfZ"
                     /locus_tag="ACHUPT_001039"
     CDS             31801..32706
                     /gene="yhfZ"
                     /locus_tag="ACHUPT_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0818706.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32723..33085
                     /locus_tag="ACHUPT_001040"
     CDS             32723..33085
                     /locus_tag="ACHUPT_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0818707.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33169..34332
                     /locus_tag="ACHUPT_001041"
     CDS             33169..34332
                     /locus_tag="ACHUPT_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR0818708.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34333..35559
                     /locus_tag="ACHUPT_001042"
     CDS             34333..35559
                     /locus_tag="ACHUPT_001042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0818709.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35556..36434
                     /locus_tag="ACHUPT_001043"
     CDS             35556..36434
                     /locus_tag="ACHUPT_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0818710.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36445..36798
                     /locus_tag="ACHUPT_001044"
     CDS             36445..36798
                     /locus_tag="ACHUPT_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0818711.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36810..38114
                     /locus_tag="ACHUPT_001045"
     CDS             36810..38114
                     /locus_tag="ACHUPT_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0818712.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38126..39211
                     /locus_tag="ACHUPT_001046"
     CDS             38126..39211
                     /locus_tag="ACHUPT_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0818713.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39371..40102)
                     /gene="frlR"
                     /locus_tag="ACHUPT_001047"
                     /gene_synonym="yhfR"
     CDS             complement(39371..40102)
                     /gene="frlR"
                     /locus_tag="ACHUPT_001047"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR0818714.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40202..40987)
                     /gene="frlD"
                     /locus_tag="ACHUPT_001048"
     CDS             complement(40202..40987)
                     /gene="frlD"
                     /locus_tag="ACHUPT_001048"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0818715.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40984..41814)
                     /gene="frlC"
                     /locus_tag="ACHUPT_001049"
     CDS             complement(40984..41814)
                     /gene="frlC"
                     /locus_tag="ACHUPT_001049"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR0818716.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41864..42886)
                     /gene="frlB"
                     /locus_tag="ACHUPT_001050"
     CDS             complement(41864..42886)
                     /gene="frlB"
                     /locus_tag="ACHUPT_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0818717.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42907..44244)
                     /gene="frlA"
                     /locus_tag="ACHUPT_001051"
                     /pseudo
     CDS             complement(42907..44244)
                     /gene="frlA"
                     /locus_tag="ACHUPT_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44539..>44705)
                     /locus_tag="ACHUPT_001052"
     CDS             complement(44539..>44705)
                     /locus_tag="ACHUPT_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0818718.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
BASE COUNT        10414 a        11345 c        12234 g        10712 t
ORIGIN      
        1 ctggttgtct ggagattcag ggggccagtc tactcgccgc agccaactct tcttctgaca
       61 gcccggtaaa tcgcatgatg tctgcaagag ggactccgga ttcaagcatt attttggcta
      121 tatgcagggc tttggattgt tccccctcct gccgcaatct ttccgcaata gtcattaaac
      181 tctccttgtg tttcggtgaa cgttcggcaa cgccgtcgat aaaatcgtta aaacgtacag
      241 cgtcgccagt ttgcagtatg taattaaaca gccctttgat ttgtctgtca ttagcgtatc
      301 cactacttaa taagcaggcc atttgctcta ccagccccat cagatcgcgt tgacgaatat
      361 gtttttgaat taactccagc agcgccatac gtcggtgctg catgatttca tcatcaggca
      421 tgacggtgac atcaatcagc ggaaatgcgg aggcataaag ctgccttgcc agtttgggat
      481 cggcgaaaca atccagccaa cacagcgaat agggataggg gctttcaata ccgtggtaaa
      541 acaacaatgg caccaccatc ggcaacgttt tgtatccagc atcaaggtga ttttgcattg
      601 cggcaatagc gtaacgcatc atgcgaaatg cgatcagttt gtttgaggtg ctttgatgtt
      661 caatcagaca atagatgtat cctggtcctt gttccgtttt caccgaccac agcacatcgg
      721 aatagctttc acgcagatcg tcatcaataa agctcctcga ctccagcttg agggttttca
      781 tatcacagag cgcgtgaatg ggtgccggta aatgaaacgc aagaaaatcg cgtgccgtct
      841 ccggttggcg taaaaagagt ttgaacagcg catcatgtgg ggtggaactc tgctttttac
      901 tcatggcgtt ccttcacctg ttgtttgaat gccgcgactc tacgcgccac tccacatctg
      961 tgcattagcg agttttcatc cctgcgagac gctttcagag gaacttacgt tgcgctgcaa
     1021 gaattgcatt tttatggaaa gccgcacgca aaaaagcccg gatgcaaacg cataccgggc
     1081 ttttcaaaac aggcgatgga gtatggttaa cgcagcgccc accactcgcg cagacaggct
     1141 ttgccttccg ggcagctttt acaactgcca gagaggcagc cgtcaggttc ttcctgaatc
     1201 cgcacggctt tgcccatgct ttccagctgt tgcagcatgg cgttaatcat tggctgcgga
     1261 gtgttcaatg tctggcttat ctgggtcgct tccatacggc cccgtaacgc cagcaaatca
     1321 cgtacctgaa taagtgaagc cattaatggc aatcaccggt ggtgctggct gcgcagcaac
     1381 tgcttaccga cttgcgggtt gccagcagtt cgatatccac ccggctacgc gcgcggcgca
     1441 gcagaccgat agctacgatg ttaaacagga ttaccgccag aatgcacacc aggctgtaag
     1501 ttgggtgctg actgtagctg gcgacctgat agaacaatgt tgccagtgag taagcgatat
     1561 tcagccccca caggatggag aagcccatcc agccacggct tgattcacgg gcgatggctc
     1621 ccatcaccga gatacatggt acatacagca ggacgaaaat caggtagctg tatgcggcag
     1681 ctgcgctacc gaatttctga tccatcacgc ccatcgcccc ggtacccatt tcaccgttgc
     1741 ctttgctagc ttcaatgggg ttcatcagta cgctaaggct gaaggtgtct ttcaggctct
     1801 gccaggtttc atctaccgca ctgaacagct cttcaccgag gttgaattcc gccggattga
     1861 actcttcgtc ctgaatattt tctgcggtgt agagggtgtt gagcgtaccc accaccactt
     1921 cttttgccat tgcgcccgta aacaggccaa ccgttgcctg ccagttatct tcatgtacgc
     1981 caatcggctt gaagacaggg gttatcaccc ggctgacgga agccagcgcc gagtcgttga
     2041 tgttatcgac gattttcccg ctcaacgaga agctgttgaa agcgctcagg aaaatgctga
     2101 cgatgataat cactttacca gcacgcagaa cgaagccctt cagacgctgc caggtctgga
     2161 taatcaggct tttaacgtgt ggtacatggt agaccggcag ctccatgaca aacggcgtcg
     2221 cttcaccgcg catgatggtg tacttgagca tcaggccagt cagcaccgcc atcacgatac
     2281 cgagcatata cagcgagaaa accgccaggg caccgttctg cccgaagaag gcagccgcga
     2341 atactgcgaa gatagccaga cgcgcgccgc aggacataaa cggtgccatc atgatggtca
     2401 tcagacgttc acgcggtgca tcaagcgtac gtgcgcccat taccgacggt acgttacaac
     2461 cgaaaccgac gatcagcggc acaaaggatt tccccggcaa gcccagcgcc tgcatcaggc
     2521 ggtccatcac aaacgctgca cgcgccatat agccggagtc ctcaaggaag gagaggaaca
     2581 ggtacatcat gccgatctgc ggcaccagtg gcagcacggt gttaataccg ccacccaggc
     2641 cctgggcgag gaagatagtc agccagtccg ggaagtggag cgtgtagcca atccattgaa
     2701 taccatgcac aaatagcgcc acggagccga cgttaaacag cggctgtaac gccccgccga
     2761 tgttgatagc cagcaggaac atcaggtaca tcacaaagag gaaaatcggc agaccgagga
     2821 aacggttgag cacgatttta tctaccgcag tggtgaaacg gctgggttct gccgtcaggg
     2881 tgttgcttac cacatcacag atggcagcaa tgcactggta acgcgcatcg gcaatgtgca
     2941 gcgccggatc atccatttca ttacgcagac gggcgagggt ggcatccaga tgctgcgacg
     3001 cttcaccggc gtaggcgcgg ctgtagatat cgccttccag catttgcagg cccagccagc
     3061 gacgttgttt cagcgggatg tcggaaggca tcacttttgc cagtgaatct gcttcgttga
     3121 gtagcggctg tgcgtaatgc accagttcca cattctcgtt agctttatag cgatcaatcg
     3181 ccagcttgag cgcttcaata ccgcgaccac gggttgaaac cagcgggatc accgggcagc
     3241 caagacgtgc cgatagagca tcaatttcaa tacgaatatt ttgcttctcg gcaatgtcga
     3301 gcatgttcag cgccacgatg cagggaatgc cgagttccag cagttgcagc gtcaggtaca
     3361 ggttacgctc aaggttagac gcatccacca cgttaatcag caggtcggcg tcgccactca
     3421 aaatgtagtg acaggcgatt tgctcatcga gcgaggtctg cgatgagatg gtggtcagag
     3481 aataggtgcc gggcaggtcc accagcgtga cctgatgatc ggtggtggag aattgccctt
     3541 ctttacgttc gacggtaacg ccagcccagt tacctacacg ctgacgtgcg ccagtgagct
     3601 ggttaaataa ggttgtcttg ccagaatttg gattaccaat taagccaatg gttaattttt
     3661 tcattgttgt tatcaccgta ttaacaggaa accgcttcca cttctaataa ggccagatct
     3721 tttttgcgta ataccaggct cacacgacgg gtttcgatat gaatggggtc gccgagtgga
     3781 gcgacgcgca ccacatcaaa agaggagcca ggtaacatgc ctagagaaag cagtttttgg
     3841 cgatatgccg ggctgatttc acgggaaaag ccagtgattt tccacgcagt atctggagtg
     3901 tattgcatag gtgcctactt gtttctcatt aactggataa ctacctcatg gggcgtttga
     3961 ccgcgacatg agaccagatg atttgccaac aaaggagaga ttaaataatc aacaacacaa
     4021 tgataatgag aatggtttct atcagcaata cttaaaatgt gtgcgaatgt tggtttgaat
     4081 aataatctga ctcaatgttg atgtggctca aggttatatc gcagcgaaaa tataattcga
     4141 atagtgagtt acccggactg gctaatgttt aattaaggtt tcagttgtta atgaagtgat
     4201 aaatattaac ggagtatatt gtgagtttgg ctgtggaata aaagtgccgg agatattctc
     4261 cggcactttt atgtcaggca aagcgttcgt tgccagatgc ggcgtgaacg ccttatcgcg
     4321 caggtttagg ggccagtccg ggcgtttaac gttttttacc catcgctgcc gccagcgcat
     4381 ccatcatcgc gctattaccg gcaggctgcg cttcacgacc gcgtggtttg gctgccgggc
     4441 ggttgttttg cgggcgttca ttaccgccgc cgcgacgaga gttggtttcg ccaggctgct
     4501 cgtccagacg catagtcagg gcgatacgtt tacgctgaag atccacttcc agcactttca
     4561 ccttcacaat gtcgcccgct ttcaccacgg tatgcggatc ttccacaaac ttgttcgaca
     4621 atgaagagat gtgaaccagg ccgtcctgat gcacgccaat atcgacaaac gcgccaaagt
     4681 tggtgacgtt ggtcactgcg ccttcgagga tcatacccgg ttgcaggtcg ttcattgtct
     4741 cgacgccatc ggcaaactga gcggttttaa attccggacg cggatcgcga cccggttttt
     4801 ccagctcttt gatgatgtca gttactgtcg gcacaccgaa tttttcatca gtaaagtcag
     4861 acgctttcag gttacgcagt tcgctgctgt tacccatcag atctttcagt gcctgctgtg
     4921 ttgctgccag aatgcgttcc accaccggat aggcttccgg atgaacggta gaggcgtcca
     4981 gcgggttatc accgtggtta atgcgcagga agcccgcgca ctgctcgaag gctttcggcc
     5041 ccagacggct gactttcagc agttgctgac ggttctggaa ctggccgttc tcatcgcgcc
     5101 aggccacgat gttttgcgcc atcatgcgcg tcaggcccgc cacgcgggtt aatagcggaa
     5161 cagaagcggt gttgagatcg acgccaacgg cgtttacgca gtcttctact actgcatcca
     5221 gtttgcgggc cagttgcgtc tggctgacgt catgctgata ctgacctacg ccgatagatt
     5281 tcggatcgat tttcaccagc tccgccagcg gatcctgcaa acggcgggcg atagataccg
     5341 caccacgcag cgaaacgtcg agatccggga actcctgcgc tgccagctcg gaagctgagt
     5401 aaaccgacgc gccagcttcg ctgacgatca ctttctgtgc ggtcactttc gggaactgct
     5461 tctgcacgtc gagatagaaa cgttcagttt cgcgggaagc tgtaccgttg ccgatcgcca
     5521 ccagttcaac gttatgtttt tcacacaagg cagcaacggt catcgctgct tttgcggctt
     5581 gtccggtgtg cgggtaaatg gtatcggtcg ccaccagttt gccagtggca tcgaccaccg
     5641 ccacttttac cccggtacgc agacccggat cgaggcccat cgttgcacgc agtccggcag
     5701 gggccgccat cagcagatcg tgcaggttac gggcaaagac gttgattgct tcatcttccg
     5761 cgcgttcgcg cacggtaccc atcagttcgg tttccagatg catcagcacc ttgatgcgcc
     5821 aggtccagct cactacgctt ttgcgccagc tatccgccgg ggcattgttc aggcgcaggc
     5881 caaggtgatc catgatgatt tgctcgcaat agctctcttt gggcggctca tcgaactgcg
     5941 gatcggcatt cagcgaaagc tggagtacgc cttcgttacg cccacggaac atcgccagcg
     6001 cgcggtgaga aggcaccgtg gacaacggtt cgtgatgatc gaaatagtcg cggaatttcg
     6061 ccccttcctc ttctttaccg ctcaccaccg tagaaaccaa atgcgcgttc ttccacagat
     6121 aatcacgcac tttcgccagc agcgtggcat cttcggcaaa ccgttccatc aggatatagc
     6181 gcgcgccatc cagcgcggct ttggtatctg ccacgccttt atcggcatca acatattgtg
     6241 cagcggcgac ttctggcgtg tgtgacggat cgctccacag caggtcagcc aacggctcaa
     6301 gccctgcttc aatggcgatt tgcccgcggg tgcggcgttt aggtttgtag ggcaggtaga
     6361 ggtcttcgag ttcggttttg cttagggtgg cgttgatggc cttcgccaga tcatcggtga
     6421 gtttgccttg ctcggaaatg gatttgagga tcgcctgacg tctctcttcc agctcgcgca
     6481 gatagctcag acgcgtttcc agattacgca gctgcgtgtc atccagaccg ccggtgattt
     6541 ccttacgata acgtgcgata aacggcacgg tattcccttc gtcaagcagg cgaacggcag
     6601 cgtcaacctg ttccgggcgc gcctgaattt cacccgcaat aatgcggcag aacgaatcat
     6661 tcatcatggt ttgagttcat cttttcggat caaaaatcag gggatagtta tacggactgg
     6721 ctggcaaaaa tgccagccat cggcaggagg ttaagactct tccttacggt ttcacgtact
     6781 cgatagcatt aacataccag ttcgcttccc cggcaggggt attcaccacc gccagatcgc
     6841 cgacttcttt tttcagcaat gcgcgggcca tcggggaatc gatagagatg taatctttac
     6901 ggccaaaaat ttcatcgtag ccgacaatac ggaaacggtg agtcacgcca tcgtcgtttt
     6961 caatctccac ccacgcgcca aaaaagactt tgccttcctg ctgaggggag taatcgacga
     7021 ttttgagatt ttccaggcat ttagtgagat agcgcacgcg acggtcgatt tcacgcagac
     7081 gctttttatt atactgatag tcagcatttt cgctgcggtc gcccagactt gcggcccagg
     7141 tcaccttttt tgtgacctcc gggcgttctt cacgccagag ataattaagc tcttgtttga
     7201 gtttttcata cccttcccgg gtaaccaggg gcgttttcat ctcgttgatt ccctttgtct
     7261 gtttgataat gcgcacattg ggtataacgt gatcatatca acagaatcaa taatgtttcg
     7321 ccgaataaat tgtatactta agctgctgtt taatatgctt tgtaacaatt taggctgaaa
     7381 ttcataccag atttagctgg tgacgaacgt gagctttttt aagaatacac gcttacaaat
     7441 tgttgcgaac ctttgggagt acaaacaatg caagagaact acaagattct ggtggtcgat
     7501 gacgacatgc gcctgcgtgc gctgctggaa cgttatctca ccgaacaagg cttccaggtt
     7561 cgaagcgtcg ctaatgcaga acagatggat cgcctgctga ctcgtgaatc tttccatctt
     7621 atggtactgg atttaatgtt acctggtgaa gatggcttgt cgatttgccg acgtcttcgt
     7681 agtcagagca acccgatgcc gatcattatg gtgacggcga aaggggaaga agtggaccgt
     7741 atcgtaggcc tggagattgg cgctgacgac tacattccaa aaccgtttaa cccgcgtgaa
     7801 ctgctggccc gtattcgtgc ggtgctgcgt cgtcaggcga acgaactgcc aggcgcaccg
     7861 tcacaggaag aggcggtaat tgctttcggt aagttcaaac ttaacctcgg tacgcgcgaa
     7921 atgttccgcg aagatgagcc gatgccgctc accagcggtg agtttgcggt actgaaggca
     7981 ctggtcagcc atccgcgtga gccgctctcc cgcgataagc tgatgaacct tgcccgtggt
     8041 cgtgaatatt ccgcaatgga acgctccatc gacgtgcaga tttcgcgtct gcgccgcatg
     8101 gtagaagaag atccagcgca tccgcgttac attcagaccg tttggggcct gggctacgtc
     8161 tttgtaccgg acggctctaa agcatgaggc gattgcgctt ctcgccacga agttcatttg
     8221 cccgtacgtt attgctcatc gtcaccttgc tgttcgccag cctggtgacg acttatctgg
     8281 tggtgctgaa cttcgcgatt ttgccgagcc tccagcagtt taataaagtc ctcgcgtacg
     8341 aagtgcgtat gttgatgacc gacaaactgc aactggagga cggcacgcag ttggttgtgc
     8401 ctcccgcttt ccgtcgggag atctaccgtg agctggggat ctctctctac tccaacgagg
     8461 ctgccgaaga ggcaggtctg cgttgggcgc aacactatga attcttaagc catcagatgg
     8521 cacagcaact gggcggcccg acggaagtgc gcgttgaggt caacaaaagt tcgcctgtcg
     8581 tctggctgaa aacctggctg tcgcccaata tctgggtacg cgtgccgctg accgaaattc
     8641 atcagggcga tttctctccg ctgttccgct atacgctggc gattatgcta ttggcgatag
     8701 gcggggcgtg gctgtttatt cgtatccaga accgaccgtt ggtcgatctc gaacacgcag
     8761 ccttgcaggt tggtaaaggg attattccgc cgccgctgcg tgaatatggc gcttcggagg
     8821 tgcgttccgt tacccgtgcc tttaaccata tggcggctgg tgttaagcaa ctggcggatg
     8881 accgcacgct gctgatggcg ggggtaagtc acgacttgcg cacgccgctg acgcgtattc
     8941 gcctggcgac tgagatgatg agcgagcagg atggctatct ggcagaatcg atcaataaag
     9001 atatcgaaga gtgcaacgcc atcattgagc agtttatcga ctacctgcgc accgggcagg
     9061 agatgccgat ggaaatggcg gatcttaatg cagtgctcgg tgaggtgatt gctgccgaaa
     9121 gtggctatga gcgggaaatt gaaaccgcgc tttaccccgg cagcattgaa gtgaaaatgc
     9181 acccgctgtc gatcaaacgc gcggtggcga atatggtggt caacgccgcc cgttatggca
     9241 atggctggat caaagtcagc agcggaacgg agccgaatcg cgcctggttc caggtggaag
     9301 atgacggtcc gggaattgcg ccggaacaac gtaagcacct gttccagccg tttgtccgcg
     9361 gcgacagtgc gcgcaccatt agcggcacgg gattagggct ggcaattgtg cagcgtatcg
     9421 tggataacca taacgggatg ctggagcttg gcaccagcga gcggggcggg ctttccattc
     9481 gcgcctggct gccagtgccg gtaacgcggg cgcagggcac gacaaaagaa gggtaaataa
     9541 acgggaggcg aaggtgcctc ccgttttgct ttctataaga tactggatag atattctcca
     9601 gcttcaaatc attacagttt cggaccagcc gctaccagcg cggcacccgc aggggtgtcg
     9661 gtgtatttat cgaagttgtc gataaacagt ttcgccaggg tttcggcttt ttcctgccac
     9721 tgttccggag aagcgtaggt gttacgcgga tcgagaatct tcgtgtctac gcccggcagt
     9781 tcggttggga tcgccaggtt aaacatcggc agagtgaagg tttctgcatt atccagcgaa
     9841 ccgttgagga tggcgtcgat aatggcgcgg gtatctttaa tcgagatacg tttgccagtg
     9901 ccgttccagc cagtgttaac cagataagcc tgcgcgcccg ccgcctgcat acgtttcacc
     9961 agcacttctg cgtactgagt cgggtgcagc gacaggaacg ccgcgccgaa gcaagcggag
    10021 aaggttggcg tcggttcggt gatgccacgc tcagtaccgg ccagtttggc ggtgaagcca
    10081 gagaggaagt gatactgggt ttgatcggca gtcaggcgag aaaccggcgg caacacgccg
    10141 aaagcatcag cagtcaggaa gataacctta gtcgcgtggc ccgctttgga aaccggctta
    10201 acgatgttat cgatgtgata gattggataa gaaacgcggg tgttctcggt ttttgaacca
    10261 tcatcaaagt cgatagtgcc atcttcacgc acggtgacgt tttccagcag cgcatcacga
    10321 cggatagcgt tgtagatttc aggttccgct tctttcgaca gcttgatagt ttttgcgtag
    10381 cagccgcctt cgaaattaaa cacgccgtca tcgtcccagc cgtgttcgtc atcgccaatc
    10441 aggcggcgtt tcgggtcggt ggaaagggtg gttttaccgg tgccggaaag gccgaagaac
    10501 accgcaacat cgcctttctc gccaacgttg gcggagcagt gcatagaagc gatacctttc
    10561 agcggcagca ggtagttcat catcgagaac atccctttct tcatttcgcc gccgtaccag
    10621 gtgccgccaa tcagctgcat gcgctcggtc aggttaaacg ccacgaagtt ttcggagttc
    10681 agaccctgtt ctttccactg cgggttagtg cacttcgcgc cgttcataac gataaagtct
    10741 ggtttgaaac ctgccagctc ttcatcgctc gggcgaataa acatgttttt gacaaaatgc
    10801 gcctgccagg ccacttcggt gatgaaacgg acggaaagac gagtatccgg gttcgcacca
    10861 cagaaagcgt cgacaacgaa cagacgtttg ccggaaagct gcctggtcac caggcctttc
    10921 agatgctgcc aggtttccgg agagagaggt ttgttgtcgt tcttaccttt gcctttgtct
    10981 gcccaccaga aagtatcgcg agtggtatcg tcacggacga tatacttatc ttttggtgaa
    11041 cgaccggtga agatcccggt atcgacggca acggcaccca gattagttaa caccccgcgc
    11101 tcataacctg tcaggctcgg atcgagctct tcctgataca gcaggtcgta gcttgggttg
    11161 taaacgatat catgtacgtc actgatacca taagcctcga gttcttgcgg gggcaaacca
    11221 ttgttaacgc gcatttcact gctccttagc caatatgtat tgcctgaata gtaaagtctt
    11281 tttgggggtg ttaaccgcga caaggctcat agatttacgt atctggagaa attcatattt
    11341 gacaaaaaac tccgtgattc ctgtcacgaa acggttgcta ttatcgcaga aatagcattt
    11401 taggtgtgga aaatgttctc aaaaggttaa atcttggttt atatggcggg aatattgtga
    11461 gtggaatcgc attcctgaat gaaattgatg caaattcaac cggtaaagta tcgattcacc
    11521 acgctaactt tttttgatta ttttctgaaa aaagcaaaat actactgccc ggatgcgata
    11581 attaacccac cttttgattc tggataacca acatggataa cgttgaactt tcaccggcaa
    11641 cacgctgggg gatgattgct accggattac ttcaggggct ggtttgctac ctgcttatcg
    11701 cctggcttgc cggaaaaaat cacagttgga ttgtttatgg cgtgcctgcg accgtcgcgt
    11761 tttcatccgt tttactcttc tccgtgatct cttttaaaca gaagcgcctt tggggatggc
    11821 tggcgctggt gtttattgcc acgacaggga tggctggcgc tggtgtttat tgccacgaca
    11881 gggatgagcg gttggctgaa gtggcaaact gatggcatga acccctggag agctgaaaag
    11941 gcgatttggg attttggctg ctatctgctg ttgatggcaa tgttgttgct accgtggata
    12001 caacaaagcc ttcgtatccg caatggcagt agccgctata gctactttta ccagtcagta
    12061 tggcataacg tacttatatt actggtgatt tttctctcca atggcctgac gtggctggtg
    12121 cttttactgt ggggtgagtt gtttaaactt gttggcatca cgtttttcaa tacgctcttt
    12181 tttgcaaccg actggtttat gtatcttaca tttggtctgg ttaccgcact ggcggtgatc
    12241 ctcgcgcgta cacagtcacg tttaatcgac tctattcaaa agttgttcac gctaatcgcc
    12301 acggggttgc tgccgttagt atcattgcta accctgatgt ttatcatcac cctgccgttt
    12361 acgggcctga gcgcgatttc tcgccacatc tccgccgccg ggttgctgtt aacgctggcc
    12421 tttttgcaat tgatcttaat ggctattgtc cgcgatccgc aaaaagcgtc acttccctgg
    12481 acggggccgt tgcgttgcct gattaaaacc gctttgctgg ttgctccgct gtatgtgttc
    12541 gtcgccacct gggggttatg gctgcgggtc gctcagtacg gctggactgt cgaccgcttg
    12601 cagggcgcgc tggcggagct ggtgttactg gtgtggtcgc tggggtattt tgtcagtatc
    12661 gtctggcgta atgggcaaaa tccccttgtt cttcagggca aagtgaacct tgcggtttcg
    12721 ttattggtgt tggtgatact ggtgcttctt aattcgccgg tgctggacag tatgcgtatt
    12781 agcgtgaaca gtcatatggc gcgttatcag agcggcaaaa acacgccaga ccaggtaact
    12841 atctacatgc tcgaacagag cggtcgctat ggacgtgcgg cgcttgagtc gctgaaaagc
    12901 gatgccgggt ttatgaaaga cccgaaacgc gcgcgggatc tgctgatggc gttagatgga
    12961 gagcaacatc ttcaggaaca ggtatcggaa aaagtattcg ccgaaaatgt gttaattgcc
    13021 cctggttctg ttaaaccgga tgcgacattc tggtcggcct taatccagga tcgctataac
    13081 gtgatgacct gtattgaaaa agacgcctgc gtactggtcg agcaagatct gaatagtgat
    13141 ggtcaggcgg agcggatcct gtttgctttt aatgatgaca gagtcattgt ctatggcttt
    13201 gactcagaca gaaaagaatg ggacgcgctt gatatgagtt tacttccgaa cgaaataacg
    13261 aaagaaaaat tactcacagc tgcgaaggat ggaaaactgg ggacgaggcc gaaagcgtgg
    13321 cgcgatctga cggtagatgg tgaaacgctg gaaataaact taagtaagtg acaacgcttt
    13381 tatcggatac gacgctgtat ctgaccaaaa taatttgcga tttatttcat aggcgctggt
    13441 aagcgcagca catcaggcca ttaagtcgta aaatccggca gagccctctc tgccggacat
    13501 actcattaat gaacttacgg atctgccgga gacgcgttgt tgcggatttc agcaatatcc
    13561 atcgcattga acagatagtg gttaccgcag taatcacaat gcatgtcaat ttcgccatct
    13621 tccgccagga tgctatcaac ttcttcatca ggcagcgttt tcagcgcatc ggcgcaacgt
    13681 tcacgcgagc aggtgcattt gaactccaca tcctgcggat cgtaaaccgt cacctcttct
    13741 tcgtgataca aacgccacaa cacttcgttt gccggtaagg tcagcagttc ttcggttttg
    13801 atggtttcgg ttagcgtcgc caggtggtca aagtcgtcct gttgggtatt ttgcgcaggc
    13861 attacctgca acaacatacc gcctgcagcc ggtttgccgt ctacgtcgcc ggtgcgaata
    13921 aacaggcgcg tcggcagctg ttcagaacgc ataaagtaat cttctaggca ggccgccagg
    13981 gtatcacctt ccagaccaac tacgccctga tagcgttcgc cttcgctcgg ggtaatggtg
    14041 atcaccacgt aaccattgcc gaccagcgtt ttcaggtcgg cattttctgg aatttcgccc
    14101 tgcacgcgcg ccacaccgcg catttgctgg ttattgttac cgttaataac cgccagattc
    14161 atcggaccgt cgccctgcag ctgtacggtg atatcaccat caaacttcag cgtagcggtt
    14221 aacaggctgg tcgcaaccag cagttctgcc agcacgtttt taacgggctg cggataatcg
    14281 tggttctcaa ggatctgttg cagggtttcc gaaacggtta ccagttcgcc gcgaacggca
    14341 aaattttcaa acagatagcg atgtaattgg tcatgttgcg gcataatcat ctctcttgca
    14401 ggtgacagtt attcactgtc gccgtgttta aatcgtaaca ggtcgcggcg ctcttttttg
    14461 tccgggcgtc ggtccgggtg cggcatggtt aaggcattaa gtttacgtgc catcgccatt
    14521 ttttcgcgtt tctctacact ttccgcagtc tcttcataca gcaaggctgc ctcgctggcg
    14581 gggcgacgct gttcagtaat cgcctttaca atcaccgtgc gttcgtcatt tccctggcgc
    14641 agagtgagcg tggcattcag ctcgacgatt ttgctcggct tgctgcgctg cccgttgtaa
    14701 tgcaccttac cgccttcaat catttcacgg gccagcgcgc gggttttata aaaacgggca
    14761 gcccatagcc atttatccag tcgaacctca acagcaggtt tctctttcat ggcgtctcct
    14821 tcacattagc gaggggatca ggcggcggta gtcattcagt gacggatggc gttgatactg
    14881 tttctcggca atcccggaat caggattagt cacgccgagg cagtaacgaa taccaaattg
    14941 cgcggcagca tcgagaatcg cttcgctgtc atcaataaac agcgttcttt cagctttcag
    15001 acccgtagct tcggccaccg catgccataa ccgctgatcc tctttcggat aaccaaatgt
    15061 gtgggtggaa agtaataaat caaggtgtgc gtccagaccc gtatgctcaa gttttaccgc
    15121 caggttgtgc ggatgagcat tggtgagcaa aattcgctgc ttaccgctgg ctttcagtgc
    15181 ctcaagaaac ggaatggtat cttcacgcag tacggcgcgc ggtcccatct cggtggtcat
    15241 cgcacagata tccagaccca gttgctcact ccagtaatca agacagtacc agtttagcgt
    15301 atgctgtacg tcgtgatatt gctggcgcat atattccatc gcttcctgtg gcgtaacccc
    15361 gtttttcgcg ccccatgttt caggcaccag cttttgccag aaatagttat cgaaggcgag
    15421 gtcgagcaac gtgccgtcca tatccagcag aacggtatct acgtcctgcc aggcaatgtt
    15481 gatatgcatg agggaaatct ccagagtgaa gcaatttgcg cgacagggta gcataacctg
    15541 ccgcgcaaac gtgttattcg ataaggcttt ctgaaggggt gatcagttgc gggttcaggc
    15601 agctttcata ataggcctga atctccatca tgcgagtgcg atgacgctgg taacgacgcc
    15661 aggcctgtac gccattgtaa atggcgctac ccagcatcgt cagtagcagc aatgtggtgc
    15721 tgagatagcg ccacaggccg gaacgatccg ggatcggatg aaggccaata tgctgcgtac
    15781 cattggcgtc agtgaagatt tttgtgacga taccttcggc gttaaacggc gtatgcatca
    15841 gcatttgtgc cagtttctgg aaagcgttcc actgttcttg cggcgggtag tcgtaaagtg
    15901 atgccgaagg ccagggctga tcaacaaaat cgctgccttc gtcgctgaca atcaggaatc
    15961 cgccgggggc cggactgttt agtgattgtg ccgctcgcgc cgtttcatgc gtgataaacg
    16021 gcgcggtgga ggttgccacc aggttatcca gcgattcagc actcaccggg cgtaataaca
    16081 cattcacgcc atcgagcttc ccggcgttgg cgcgttttac cagcgcgtcc cagtctttac
    16141 tgttgccgag attgaccagc gcatttttca gtcgcacaca gtcatctttg gcagaacata
    16201 aatccgctgt cttcagtaca atgtcgccaa aatcatcaag caataccatg ccggattttt
    16261 gaatcgctga acgtaatgag gcactgacgc gagattcatc ttccggttta gggtgcagct
    16321 ggcgattaac tgcttcagtc aatgccgtcg ctttgttgac cagttcagat tctggtaatg
    16381 gtaatgagcg ggcgtcattc cagatgatct gcgagcagtc aaacggtaaa aaaggtgaat
    16441 tggttttcgc gctccaggtt ccggaagtgc gaatattaca cattcccgta ccgctaatac
    16501 gcaatgtatc gcctacccgc acgccagcgt cagccagttg ttttacgctg gtggcttcaa
    16561 tggtctgcgc gcctttcatc catgagagag tgaatttcag cggcatatcc agcgggatcc
    16621 agaataacag cataaacagc actagcagcg agcccgcagc gataatcgta ctgcgcagcc
    16681 agtgctgtaa cggaaagttt tttacttcat catgcagcga aagatatcgc ccctgacgca
    16741 ctacgtggcg gtcgagatag atatcaatat cggtttgttg accgagatcc tgagcaatgt
    16801 atggctgcca gtgtgcggga tagaccaggt cgataatacc gagtgaaata ttgttgatct
    16861 gttcctgatc gttttcgcca aacaatcccc aacgacgggg tgtaccgcgc agacaatgaa
    16921 tttctcgcag ggaggatttt gccgggggcg cgaatagccc ccacagacct gcgcccagca
    16981 gcagtaatgc gccgcctgcc agccacggaa caaatacatc cggggtaatc aggcagaaaa
    17041 agaacatcag gaaggaggcg acgatcagca acgcttcacg cagcccgcgc ggacgactca
    17101 gcgcatattc ttcatgcgtt tctttgcgaa tattgagtag ctcgatttgc tcactctctt
    17161 ccccgcgaat cgacgcctgc gtggacggaa caggttgcaa ggcatagccg cgcgtttcct
    17221 gcatgtactc ctgcagcgta tgaccgttga gtgaaataac caacggcagc gaatcggtat
    17281 gaatcagttc aacggtattc tcatcgttga tgtactgttc ccagaagggg ggcaggtgga
    17341 cttctaccga atcgaggtag taacgccatt tattcggatc gtcggtagag atgccgtaac
    17401 gtgtgatagc gtgtgtcagc atcatgacgt tgttgctttc ggcattcagc gccagagaga
    17461 tcggtgccgc gctagcttcc gttgggccag gcacctgtaa tacctgcgtc aggctctcaa
    17521 gataattttc aacggcgcta cgttcttcag gtgtgagttt acgcgtttgc gcatccgcga
    17581 aggcgttggt ccagggcagc tgacgccgtc tggatcgcac ttttatcagc catcccgcaa
    17641 gtagtgagca ggccagcaaa gcagctaaaa aaatcacaat ggtgctcatg ctttccccat
    17701 cttacttagt ctgtcaggcg tggtcagtcg caccggacac aatacatgat tctgcggttg
    17761 gctatcggca agcgtggagc ttaacttgag catcattaag cacatcacag taaccgctga
    17821 tcatagcaaa ccctctgaag gcggaatatc agcaaattcc tggagttatt tcaacctatt
    17881 gacttttaat ttgatttgag gacgattatt tattaagtta cataaatgta aataacatgc
    17941 aattcacatt gccgaaagcg tgtccgatat cgcacaataa ccgctgattt cacagcatag
    18001 atcccacgat gaacaaatca ttacaaaaac ccaccattct gaatgttgaa actgtagccc
    18061 gttcccgact gtttaccgtc gagagcgtgg atctggagtt cagcaatggc gtgcggcgtg
    18121 tttatgaacg aatgcgtcca accaaccggg aagcagtgat gattgtgccg attgtggacg
    18181 atcacctgat cctgatccgc gaatacgcag tgggaactga atcctacgaa ttaggttttt
    18241 cgaaaggatt aattgatccg ggtgaaagcg tctacgaagc cgctaaccgc gagctaaaag
    18301 aagaggttgg atttggtgcg aacgatctga cttttttgaa gaagctcagc atggcaccgt
    18361 cttacttttc cagcaaaatg aatatcgtgg tcgcgcaaga tctctacccg gaatcgctgg
    18421 aaggcgatga gccagagccg ctaccacagg tgcgctggcc gctggcgcat atgatggatt
    18481 tgctggaaga ccctgacttc aacgaagcgc gtaatgtcag cgcgctgttc ctcgtgcgcg
    18541 aatggttgaa agggcagggg cgagtgtaaa tgcgtgaagt ttgcctgatg cacaacgctt
    18601 atcaggctta tggggttcct gcaatatatt gatttttcac gattttgtta gccggataac
    18661 gcgttcaccc gcatccggca taaacaaatg cactttgtca gccaactgaa aaggcgccga
    18721 agcgcctttt taatcagaac aattcctgtg cctcaccatt atcgataatg gtcgttccca
    18781 cctcgtgcac cgcctgttgt gtcggctgcg taccttcgat gaaatactct tcgcggctgt
    18841 tgccaccatt agctaactgc ccggtgctgc gatcgatatt caccgtcaca atacccggag
    18901 gcggcgtcag cggctgctcc ggcacacctt caagaacggc tttcatataa gcgtcccatg
    18961 caggctgggc actcttggca ccgccttcgt aacctgagat ctgatcttta atcgctccgg
    19021 aagccgttgt atgaccgaga ttacgacggt gatcatcaaa gccaatccag accgaggtca
    19081 caacgcccgg accgtaaccc gagaaccacg catctttcga actgttagtg gtaccggttt
    19141 tcccgccgat atcgcgacgc tgcaaatcac gacctgcacg ccagccggta ccctgccagc
    19201 ctggttcacc aaagatattg gtgttcaaag cactcttaat caggaatgcc agcggagtgt
    19261 tgatgacgtg cggtgcgtac tcctgcgcgc cagtcttcgc cactaacgcc tgatttgcct
    19321 gctccagctg cggcattggt acagaaacat tctgctgctc gcgggagata gcgacatctt
    19381 caacatcgtt attttccagc acgttcgatt tctgcgtatc accgtaaatc actggaatat
    19441 cgcattccgg gcaggctact ttcggtttcg cttcgaaaat cacgccgccc tgatcgtttt
    19501 caattttgct gataaaccac gggtccacta ggaagccgcc gttcgccatg accgcgtagc
    19561 cgcgcgccac ctgcattggg gtgaaggacg ctgaacccag cgccagcgat tcggtgtgga
    19621 caatgttttg cgccgggaag ccgaagcgtt gcagatattc tgcagcgtag tcgacgccca
    19681 tcgcccgcat tgcacgtacc atcaccacgt ttttcgactg acccagcccc tgacgtaagc
    19741 gaattggacc agcatactgc ggtggtgagt tcttcggctg ccagtcagaa ccggcacctg
    19801 catcccagcg agaaattggc acatcgttca acatacttgc cagcgtcaga cctttatcca
    19861 tcgccgcggt gtagaggaac ggtttgatgt tggaacccac ctgacgcagt gcctgggtgg
    19921 cgcggttaaa cttgctctga ttgaaatcaa agccaccgac cagcgccata acggcaccat
    19981 tttgcggatt gatcgacacc agcgccgagt tcacttccgg cacttgtgcc agccaccatg
    20041 catcgccaac ctgacgaacc cagatttgct gacccgtttg cagaacatcg gtcactttac
    20101 gcggcgtcgg tccctgctga gtatccgaac ggtaaggacg cgcccagcga acgccttcca
    20161 tactcaatgc gacggtcgac ccgtccgcca gcatcgccgt cgctccctga ggattggcgc
    20221 tggtgactgc ggcaggcagc agcggaccat aggtcggcag cgccttcagc gtatcggtaa
    20281 tcttgttgtt atcccacgcc gactcgccca ctttccacag cacatttgcc gggccgcgat
    20341 agccgtggcg catgtcgtag tccagcacgt tattacgtac cgcctgctgc gcggcctgct
    20401 gcactttgcg ggtgatggtg gtgtaaatgc gataaccgtc ttcataggca ctttcgccat
    20461 aacggttata catctcctgg cgcaccattt cgctcaggta cggcgcagag aaagcaatct
    20521 ccggcgcgtg ataattagcg ttaatcgcct cagtgcgtgt ctgatcgaac tgttgctggg
    20581 tgatataccc ttcatccagc atccgcgaca gtacgacgtt acgccgcgcg acggcacgat
    20641 ccattgagta gagcggatta aaggtggaag gcgctttcgg caacccggct atcaccgcca
    20701 tttcgttcag cgtcagttgg tcgaccgttt taccgaaata gacttgtgcc gcagcaccga
    20761 caccataggc gcggtaacca aggtaaatct tgttcagata aagctcgagg atttcgtctt
    20821 tcgtcaacaa ctgttcaatg cgaatcgcga ggaagacttc cttaatctta cgcatcatcg
    20881 tgcgttctgg actgaggaag aaatttctcg ccagctgctg ggtaatggta ctcgcccctt
    20941 gtgacgcgtg gccggagaac agcgccacac ttgccgcacg gaagatcccc accgggtcaa
    21001 cgccgtgatg ctcgtagaag cggctgtctt ccgtcgcgat aaaggctttc accatctccg
    21061 gtgggatttg atccaacgta accggaatac gacgtttctc accgtattga gcaatcagct
    21121 cgccatcggc gctgtaaatc tgcatcggaa tttgcaggcg aacatctttt aatgtcacca
    21181 catccggcag ttgtggctcg atgtagcggt ataggccata aatcgagcct gctcccagca
    21241 gaatgcaaca gactgcaagg atcaaaaaat actttacgaa cttcactgga aatttcccat
    21301 ttagtttcat ttgggcagtt tataaacaaa cgcgcggtag tataaaggca agccagacgc
    21361 attgatatac ccgtcagagt gacgggtgat aaggagatca tcacaatgac atttaagatc
    21421 tggcaaattg gtttgcattt acaacagcaa gaagcggcag cggttgcgat cgtgcggggc
    21481 gcaaaagaat gctttttgca acgctggtgg cggttgccgc tggcgcacga cattatcaaa
    21541 gatgggcgga ttgttgatgc gcagcagctg gctaaaacgt tgttaccgtg gagtcgcgaa
    21601 ctgccgcagc gtcatcacat tatgttggcg tttcccgcca gtcgcacatt acagcggtca
    21661 tttccgcgcc cgtcgatgtc ccttggtgag cgggagcaaa cggcctggct gtcagggacg
    21721 atggcccgcg agctggatat ggatccggac tccctgcgct tcgattacag cgaagactca
    21781 ctcagccccg cttataacgt gactgccgcg caaagcaaag agctggcaac gctgcttacg
    21841 ctggcagaaa ggttgcgtgt tcatgttagt gcgatcaccc cggatgccag tgcattacag
    21901 cgattcctgc cttttttacc ttctcatcag caatgtctgg cctggcgtga taacgaacag
    21961 tggctgtggg cgacgcgcta tagctggggg cgcaaactgg cggtggggat gactagcgcg
    22021 aaggagctgg cggcagcgtt atccgttgat cccgacagcg tcgcgatatg tggcgaaggc
    22081 ggatttgacc cctgggaggc cgtttctgtt cgtcagccgc cgctaccgcc gcctggtgga
    22141 gactttgcca tcgcgctggg gctggcgctt gggaaggcgt actgatgaat ccgccaatta
    22201 attttttgcc ctggcgacag caacgccgga ccgcttttct gcgtttctgg ttgctgatgt
    22261 tcgttgcgcc tctgctgctg gccgtcggga taacgctaat actgcgtctg acaggcagcg
    22321 ccgaagcgcg catagacgca gttttgcttc aggcggaaca acaactcgcc cgcagcttac
    22381 agataacgaa gccacgtttg ctggagcaac agcagttacg cgaacagcgt tcgcaacggc
    22441 agcgccagcg acaatttacc cgcgactggc aatctgcgct ggaagcactg gcggcgcttt
    22501 tacccgagca cgcctggctg acaacgataa gctggcagca gggaacgctg gagatcaagg
    22561 ggctgacaac aagcattacc gcgttaaacg cactagaaac gtcactccgc caggatgcgt
    22621 cttttcatct caatcagcgg ggagccacgc agcaggatgc gcagggacgc tggcaatttg
    22681 agtatcagtt aacaaggaag gttagcgatg aacatgttct ttgactggtg gttcgccaca
    22741 tcaccccgcc tccgccagtt ttgctgggca ttctggttgc tgatgttagt tacgctcatt
    22801 tttctatcat cgacacacca tgaagagcgc gacgcattaa ttcgactacg ggcaagtcat
    22861 caccagcagt gggccgcact gtatcgcctg gtagacacca ctcccttcag cgaggaaaaa
    22921 acgctgccct tttcgccact ggattttcag ttatccggcg cgcaactggt ttcctggcat
    22981 ccatccgcgc agggaggtga gttggcgttg aaaacgctgt gggaagcagt gccgtcggca
    23041 tttacacggc tggcagagcg caacgtcagc gtgagccgtt tttcgttaag cgtggaaggt
    23101 gatgatcttt tgttcacgct acaactggag atgccgcatg agggttaaac gctggttgtt
    23161 ggcaggtatt gcattgtgcc ttttaaccgg tatgcgtgac ccttttaaat cgccggaaga
    23221 tctatatgcc ggattagcga acttagccag tggcgctatc aggggatggt agggcgaggc
    23281 gagcgcatca tcggtgtaat aaaagacggg caaaagaaat ggcgacgggt gcagcaaaac
    23341 gatgtgctgg aaaacggctg gacaatttta cagctgacgc cagacgtact aacgctgggt
    23401 accgggacaa actgcgaacc gccacaatgg ttgtggcaac ggcaaggaga tacaaatgaa
    23461 gcaatggata gccgcactac tgttgatgct gatacccggc gtacaggcgg caaagccgca
    23521 aaaagtgacg ctgatggtgg atgacgttcc ggtagctcag gtgttgcagg cgctggctga
    23581 acaggagaag ttgaacctgg tcgtgtcgcc agacgtcagc ggtacggtgt cgttacatct
    23641 aacagatgtt ccctggaagc aggcactaca aactgtagtg aaaagcgccg gactgataac
    23701 gcggcaggaa ggcaacattc tctcagtgca ttccattgcc tggcagaatg acaatatcgc
    23761 ccgccaggag gcggagcagg cgcgggcgca ggcaaatctg ccgctggaaa atcgcagtat
    23821 aaccctgcaa tacgccgacg cgggagaact ggcgaaagcg ggggagaagc tactgagtgc
    23881 caaagggagt atgaccgtcg ataaacgcac caatcgcctt ttgctacgag ataacaaaac
    23941 ggcgttaagc gcgcttgaac agtgggtagc gcaaatggat ctgccggtcg ggcaggttga
    24001 gctgtcggcg catattgtca ccattaatga aaaaagtttg cgtgagttag gcgtgaaatg
    24061 gacgctggcc gatgcgcaac acgctggtgg cgttgggcaa gtcaccacgc ttggtagcga
    24121 cctctccgta gcgacggcga caacgcatgt cggttttaac attgggcgca tcaacggacg
    24181 cttgctggat cttgagcttt ccgcgctcga acaaaaacag cagctggata ttatcgccag
    24241 tccgcgtctg ctggcctcac atcttcagcc tgccagcatt aaacaggaga gcgaaattcc
    24301 atatcaggtt tccagcgggg aaagtggcgc gacgtcggtg gaatttaaag aggccgtcct
    24361 ggggatggag gtcatgccca cggtgttaca aaaaggtcgc atccggctga aattacacat
    24421 cagccagaac gttccggggc aggtgctaca gcaggccgat ggcgaagtgc tggcgattga
    24481 taagcaggag atcgaaacgc aggtcgaggt aaaaagcgga gaaacgttgg cgctgggcgg
    24541 catttttacc cgtaaaaata aatcgggtca ggatagcgta ccgttgcttg gcgacattcc
    24601 ctggttcggg caattatttc gtcatgacgg aaaagaagat gaacgacgcg agttagtggt
    24661 gtttatcacg ccacgactgg tttccagtga gtaaacagcc gtaaaagcgg taatgttttt
    24721 atgctgaacg tgtttcatct atttgacgcg cgcaggtatt tagcatacaa ggagtaccga
    24781 tttgagagtt ggtgctcttc gctgcctgcg ttccatgatg atgatttatc attcaggcgg
    24841 cattttgctg tcttttttac gctaatctta cccggtgatt tatcgccaga gcggtggtag
    24901 caaggcagcg cgcttgcagc gaccagatat gcagagggat gggtgattta ttcagttgcc
    24961 aaacccgctg gagtattgag ataattttca gtctgactct cgcaatatct tatgaggttt
    25021 cagttcatgt cctgcggcgc tctctgagcg aagcgggttt atcattaacg aatagtctta
    25081 gtagtaccga aaaaatggca gagaaacgca atatctttct ggttgggcct atgggtgccg
    25141 gaaaaagcac tattgggcgc cagttagctc aacaactcaa tatggaattt tacgattccg
    25201 atcaagagat tgagaaacga accggagctg atgtgggctg ggttttcgat ttagaaggcg
    25261 aagaaggctt ccgcgatcgc gaagaaaagg tcatcaatga gttgaccgag aaacagggta
    25321 ttgtgctggc tactggcggc ggctctgtga aatcccgtga aacgcgtaac cgtctttccg
    25381 ctcgtggcgt tgtcgtttat cttgaaacga ccatcgaaaa gcaacttgca cgcacgcagc
    25441 gtgataaaaa acgcccgttg ctgcacgttg aaacaccgcc gcgtgaagtt ctggaagcgt
    25501 tggccaatga acgcaatccg ctgtatgaag agattgccga cgtgaccatt cgtactgatg
    25561 atcaaagcgc taaagtggtt gcaaaccaga ttattcacat gctggaaagc aactaattct
    25621 ggctttatat acactcgtct gcgggtacag taattaaggt ggatgtcgcg ttatggagag
    25681 gattgtcgtt actctcgggg aacgtagtta cccaattacc atcgcatctg gtttgtttaa
    25741 tgaaccagct tcattcttac cgctgaaatc gggcgagcag gtcatgttgg tcaccagcga
    25801 aaccctggct cctctgtatc tcgataaggt ccgcggcgta cttgaacagg cgggtgttaa
    25861 cgtcgatagc gttatcctcc ctgacggcga gcagtataaa agcctagctg tactcgatac
    25921 cgtctttacg gcgttgttac aaaagccgca tggtcgcgat actacgctgg tggcgcttgg
    25981 cggcggcgta gtgggcgatc tgaccggctt cgcggcggcg agttatcagc gcggtgttcg
    26041 tttcattcaa gtcccgacga cgttactgtc gcaggtcgat tcctccgttg gcggcaaaac
    26101 tgcggtcaac catcccctcg gtaaaaacat gattggcgcg ttctaccagc ctgcttcagt
    26161 ggtggtggat ctcgactgtc tgaaaacgct tcccccgcgt gagttagcgt cggggctggc
    26221 agaagtcatc aaatacggca ttattcttga cggtgcgttt tttaactggc tggaagagaa
    26281 tctggatgcg ttgttgcgtc tggacggtcc ggcaatggcg tactgtattc gccgttgttg
    26341 tgaactgaag gcagaagttg tcgccgccga cgagcgcgaa accgggttac gtgctttact
    26401 gaatctggga cacacctttg gtcatgccat tgaagctgaa atggggtatg gcaattggtt
    26461 acatggtgaa gcggtcgctg cgggtatggt gatggcggcg cggacgtcgg aacgtctcgg
    26521 gcagtttagt tctgccgaaa cgcagcgtat tataaccctg ctcacgcggg ctgggttacc
    26581 ggtcaatggg ccgcgcgaaa tgtccgcgca ggcgtattta ccgcatatgc tgcgtgacaa
    26641 gaaagtcctt gcgggagaga tgcgcttaat tcttccgttg gcaattggta agagtgaagt
    26701 tcgcagcggc gtttcgcacg agcttgttct taacgccatt gccgattgtc aatcagcgta
    26761 acaacaagaa aggtcaggcc gcttatcaag cggtctatta gcttcaggtt aattgcaacg
    26821 tggtaagcat taacctttta gtggggtgtt aaatggatga attcaaacca gaagacgagc
    26881 tgaaacccga tcccagcgat cgtcgtactg gtcgttctcg tcaatcttct gaacgttctg
    26941 agcgtactga acgtggcgaa ccgcagatca attttgatga tattgaactt gatgacactg
    27001 acgatcgccg tccgactcgt gcgcaaaaag agcgcaatga ggaaccggaa atcgaagaag
    27061 aaattgacga atccgaagat gaaaccgtgg atgaagagcg cgtagagcgt cgtccgcgta
    27121 agcgcaaaaa agcagccagt aaacccgctt ctcgtcagta tatgatgatg ggcgtcggca
    27181 ttctggttct actgctgttg atcatcggta tcggttctgc gctaaaagcc ccctcgacca
    27241 cttccagcga tcaaaccgcg tctggcgaga agagtattga tcttgctggc aatgcgaccg
    27301 atcaggcgaa tggcgtgcag ccagcgccgg gaaccacgtc tgcggaaaat actcagcagg
    27361 atgtttctct gccaccgatc tcttctacgc cgactcaagg gcaaaccccg gtggcaacgg
    27421 atggtcaaca acgtgttgaa gtgcagggtg acctgaacaa tgcgctgacc cagccacaaa
    27481 atcagcaaca gctgaacaat gtggcggtca attccacgtt gccgactgaa cccgcaacgg
    27541 ttgcgcctgt tcgcaatggc aatgcatcgc gtgacacggc gaaaacgcaa accgctgaac
    27601 gtccgtccac tacgcgccca gttcgtcagc aggcggtgat tgaaccgaaa aaaccgcaag
    27661 caaccgtgaa aacggagccg aagccggtag cacagacgcc gaagcgtact gaacctgaac
    27721 cagctgcccc tgtggcgagc acgaaggcac cggctgcgac ttctacgcca gcaccaaaag
    27781 agacggcgac tacggctcca gtacagacgg catccccggc gcaaaccacg gcaacaccag
    27841 ccgctggagg gaagaccgca ggtaatgttg gttcgttgaa atcggcaccg tccagccatt
    27901 acactctgca gctgagcagt tcctctaact acgacaacct gaacggttgg gcgaagaaag
    27961 agaatctgaa aaactacgtt gtctatgaaa cgacgcgtaa tggtcagccg tggtatgtcc
    28021 tggtttctgg cgtgtacgct tcgaaagaag aggcgaaaaa agcggtatct acattgccag
    28081 cagatgttca ggccaaaaac ccgtgggcga aaccgctgcg tcaggtacag gccgatctga
    28141 agtaatcaag gttatctccc gcaatggttt atcgttgcgg gagctgcctg aagcgctgga
    28201 tgctgtcgga gctttctcca cagccggaga aggtgtaatt agttaatcgg catgaagaaa
    28261 aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgatga tattaaacgg
    28321 catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc ggtgtttctc
    28381 aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat cagtctctat
    28441 aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct gtttgttccc
    28501 gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa aagccaggat
    28561 ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa cggcctgtgt
    28621 cgttacaatc tgcgcggtga gtttaacgtg ccgtttggcc gctacaaaaa accctatttc
    28681 ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt ctattgtgag
    28741 tcttacgccg atagcatgga gcgcgcagat gatgcatccg tcgtctattg cgatccgcct
    28801 tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag ttttacgctt
    28861 gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca tattccagtg
    28921 ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc aaaattgcat
    28981 gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa ggtggacgaa
    29041 ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataatt ctcaaggaga
    29101 agcggatgaa acagtatttg attgccccct caattctgtc ggctgatttt gcccgcctgg
    29161 gtgaagatac cgcaaaagcc ctggcagctg gcgctgatgt cgtgcatttt gacgtcatgg
    29221 ataaccacta tgttcccaat ctgacgattg ggccaatggt gctgaaatcc ttgcgtaact
    29281 acggcattac cgcccctatc gacgtacatc tgatggtgaa acccgtcgat cgcattgtgc
    29341 ctgatttcgc tgccgctggt gccagcatca ttacctttca tccagaagcc tccgagcatg
    29401 ttgaccgcac gctgcaactg attaaagaaa atggctgtaa agcgggtctg gtatttaacc
    29461 cggcgacacc tctgagctat ctggattacg tgatggataa gctggatgtg atcctgctga
    29521 tgtccgtcaa ccctggtttc ggcggtcagt ctttcattcc tcaaacactg gataaactgc
    29581 gcgaagtacg tcgccgtatc gacgagtctg gctttgacat tcgtctggaa gtggacggtg
    29641 gcgtgaaggt gaacaacatt ggcgaaatcg ctgcggcggg cgcggatatg ttcgtcgccg
    29701 gttcggcaat cttcgaccag ccagactata aaaaagtcat tgatgaaatg cgcagtgaac
    29761 tggcaaaggt aagtcatgaa taagtttgaa gatattcgcg gcgtcgcttt tgatcttgat
    29821 ggtacgctgg tcgacagtgc tcctggtctt gctgctgcgg tagatatggc gctgtatgcg
    29881 ctggagttgc ccgtcgcagg tgaagaacgc gttattacct ggattggtaa cggcgcagat
    29941 gttctgatgg agcgcgcatt gacctgggcg cgtcaggaac gtgcgactct gcgtaaaaca
    30001 atggggaaac cgcccgttga tgacgacatt ccggcagaag aacaggtacg tattctgcgt
    30061 aaactgttcg atcgctacta tggcgaggtt gccgaagagg ggacgttttt gttcccgcac
    30121 gttgccgata cgttgggcgc gttgcaggct aaaggcctgc cgctaggctt ggtcaccaac
    30181 aaaccgacgc cgttcgtcgc gccgctgctc gaagccttag atattgccaa atactttagc
    30241 gtggttatcg gcggcgatga tgtgcaaaac aaaaaaccgc atccggaccc gctgttactg
    30301 gtggctgagc ggatgggaat tgccccacaa cagatgctgt ttgtcggcga ctcacggaat
    30361 gatattcagg cggcaaaagc ggcaggttgc ccatcagttg gcttaaccta cggatacaac
    30421 tacggcgagg ctatcgatct cagccagcct gatgtaattt atcagtctat aaatgacctt
    30481 ctgcccgcat tagggcttcc gcatagcgaa aatcaggaat cgaaaaatga ctaagcccat
    30541 cgtttttagt ggcgcacagc cctcaggtga attgaccatt ggtaactaca tgggtgcgct
    30601 gcgtcagtgg gtaaacatgc aggatgacta ccattgcatt tactgtatcg ttgaccaaca
    30661 cgcgatcacc gtgcgccagg atgcacagaa gctgcgtaaa gcgacgttgg atacgctggc
    30721 cttgtatctg gcttgtggta tcgatcctga gaaaagcacc atttttgttc agtcccacgt
    30781 accagaacat gcgcagttag gctgggcact gaactgctat acctacttcg gcgaactgag
    30841 tcgcatgacg cagtttaaag ataaatctgc gcgttatgcc gagaacatca acgctggtct
    30901 gtttggctat ccggtgctga tggctgcgga catcctgctg tatcaaacta atctggtacc
    30961 ggtgggtgaa gaccagaaac agcacctgga actgagtcgc gatatcgccc agcgtttcaa
    31021 cgcgctgtat ggcgagatct ttaaggtgcc ggagccgttt attccgaaat ctggcgcgcg
    31081 cgtaatgtcg ctgctggagc cgaccaagaa gatgtccaag tctgacgata accgcaataa
    31141 cgttatcggc ctgctggaag atccgaaatc ggtagtgaag aaaatcaaac gcgcggtcac
    31201 tgactccgac gagccgccgg tagttcgcta cgatgtgcag aacaaagcgg gcgtttccaa
    31261 cctgctggat atcctttctg cggtaacggg ccagagcatc ccagaactgg aaaaacagtt
    31321 cgaaggcaag atgtatggtc atctgaaagg tgaagtggct gatgccgttt ccggtatgct
    31381 gactgaattg caggaacgct atcaccgttt ccgcaacgat gaagccttcc tgcaacaggt
    31441 gatgaaagac ggcgcagaaa aagccagtgt acacgcttcc cgtacgctga aagcggtgta
    31501 cgaagcgatt ggttttgtgg cgaagccgta atatcaaaac gctcggacgg tcccctcgcc
    31561 cctttgggga gagggttagg gtgaggggag caggcaagcg ccagtgggaa ttaccctttg
    31621 ctcgcattgt cgaataaatc acttcgtcat cactctgaaa ccggggaaac ccggtttttt
    31681 tatcctcttt tcgcaacaac ccgaaaaaat gtgaagcgcc tcgccgtttc cacatctcgt
    31741 cgtcgccact tatcttcatt ttaatgaaga tataaatatt cattttattg aaaatatatt
    31801 atgcgtcgaa cgtttatcaa aaaagaaggc gtcgtcatca cgacactggc ccgttatttg
    31861 ttgggtgaaa aatgcggtaa tcgactgaaa accatagatg agctggcaac tgaatgccgt
    31921 tcatcggttg gcctgacgca ggccgcgttg aaaacgctgg aatcaagcgg agcgatacgg
    31981 attgaacgcc gtgggcgcaa tggcagttat cttgtcgaga tggataacaa agcattgctg
    32041 agtcatgtgg atatcaacaa cgtggtatgt gcgatgcctt tgccctatac ccgcttgtac
    32101 gaaggtttag cgagcggatt gaaagcccag tttgatggca ttccttttta ctatgcgcat
    32161 atgcgcggcg cggatattcg cgtggagtgt ttacttaacg gtgtctatga catggcagta
    32221 gtttcccgcc tggcggcgga aagttatctc agccaaaaca atttgtgcat cgccctcgag
    32281 cttggcccgc atacgtatgt tggtgaacat caactgatat gccgcaaagg tgagtctgga
    32341 aatgttaagc gagtaggtct cgatagtcgt tcggcggatc agaaaatcat gaccgatgtc
    32401 ttttttggcg atagtgatgt ggaacgagtc gatctctctt atcacgagag tttacaacgc
    32461 attgttaaag gcgatgtcga tgcggtgatc tggaacgtgg tggcggaaaa cgaactgacc
    32521 atgctgggat tagaggcgac gccgctaaca gacgatccgc gatttttaca ggccaccgaa
    32581 gccgtggtcc tgacgcgagt cgatgattac ccaatgcaac aattgctgcg tgctgttgta
    32641 gataaacacg ccctgcttgc ccatcaacaa cgggtggtga gtggggaaca ggaacccagt
    32701 tattaaacga aaggcatctg atatggaaac cagactcaac ctgctttgcg aggcaggcgt
    32761 tattgataag gacgtctgca aaggcatgat gcaggtcgtc aacgtactgg aaaaagagtg
    32821 ccatctgccg gtgcgcagtg agcaaggaac gatggcgatg acacatatgg cgagtgcact
    32881 gatgcgcagt cgccgtggtg aagaaataga gccgctggat aacgagttgc tggcggaact
    32941 ggcgcaatcc agccactggc aagccgttgt gcaattgcat caggagttgt tgaaggaatt
    33001 cgctctggaa gttaacccgt gtgaagaagg ctattttctg gccaatcttt atggattatg
    33061 gatggctgct aacgaagagg tttgaagctc ccgacaaatc cacgtcgcac aacctaaata
    33121 ttcaaaaaaa tgaatattta attcaaggaa cagaaacgag gcaacaagat gtttgtagaa
    33181 gcattgaaac gccagaaccc ggcgctgatt tccgccgcac taagtttgtg gcagcagggc
    33241 aaaatcgccc cggacagctg ggtgatcgac gtggatcaga tactggaaaa cggtaagcgg
    33301 ctcattgaga cggcgcggct ttacggcatt gaactgtatc tgatgaccaa gcaatttggt
    33361 cgtaatccgt ggctggcgga aaaattgctg gcattaggct acagcggcat tgtggcggtg
    33421 gattacaaag aggcgcgagt catgcgccgc gctggtttgc cagtggcgca tcaggggcat
    33481 ctggtacaaa tcccttgtca tcaggttgct gacgccgttg aacagggcac cgacatcatc
    33541 accgtgttta ctctcgacaa agcgcgggaa gtttctgcgg cagcggttaa ggccggacgg
    33601 gtccagcctg tgctgctcaa agtttatagc gacgatgatt ttctttatcc ggggcaggag
    33661 agcggttttg tacagcattc gctgcacgag gtgattgctg aaattaagaa actgccgggg
    33721 ctgcatttag ccggactaac ccatttccct tgcctgcttt gggatgaggc tgctgggaaa
    33781 gttttgccaa caccgaatct tcacacgctg gtacaagcac gggatcaact ggcgaaatct
    33841 ggtattgcga ttgagcaact gaacgtacct tcagcgacca gctgcacttc gctgccattg
    33901 ctggcggaat acggtgcgac tcatgccgaa cccggtcatg cgctgaccgg tactattccg
    33961 gcaaaccagc agggcgatca gcctgaacgt atcgcgatgc tctggttaag tgaaatctcc
    34021 catcatttcc gtggcgacag ctactgctac ggcggcggtt actaccgtcg tggtcatgcg
    34081 caacatgcgc tggtgtttac gccagaaaat caaaagatta ctgaaaccaa tctcaaaact
    34141 gtggatgaca gcagtatcga ctacaccctg ccgctggcag gcgagtatcc ggtgagcagt
    34201 gcagtggtgc tctgttttcg cacgcagatt tttgtcaccc gtagcgatgt ggtgctggtg
    34261 tccggtattc agcgcggcga accagaaata gttggtcgtt atgacagcct cggcaaccct
    34321 ctggaggcat aaatggcgcg atttgtggtg ttagtgattg atagctttgg cgtaggggca
    34381 atgaaagatg tcacgctggt gcgcccgcaa gatgccgggg cgaatacctg tggtcacatc
    34441 ctgagccagt tgccgcattt gcagctacca acgctggaga agctggggct aatcaacaca
    34501 ttgggttatg cgccaggcga tatgcagccg tcagattccg caacctgggg cgtggcagag
    34561 ctgcaacatg aaggtggcga tacctttatg gggcatcagg aaattttagg cacgcgcccg
    34621 ttaccgccgc tgcggatgcc ttttcgcgat gtgattgacc gtgttgagca ggcattagtt
    34681 tccgctggct ggcaggtgga gcgccgtggc gatgatctgc aatttctgtc ggtcaatcag
    34741 gcggttgcga ttggcgataa tctcgaggcg gatttaggcc aagtctataa cattaccgcc
    34801 aatctctctg tgatctcttt tgacgacgca atcaaaattg gtcgtatcgt gcgtgaccag
    34861 gtacaggtcg gtcgggtcat tacatttggc ggcctgttaa ccaacagtca gcgtattctc
    34921 gatgccgcag aaagcaaaga agggcgcttt attggtatta atgcgccgcg ttctggcgct
    34981 tatgacaacg gcttccaggt cgtgcatatg ggctacggtg ttgatgaaaa agtgcaggtg
    35041 ccgcaaaaac tgtatgaagc gggcgtgcca accgtgctgg tgggtaaggt ggcagatatc
    35101 gtcagcaatc cttatggtgt gagctggcaa aacctggtgg atagccagcg gattatggat
    35161 atcaccctcg acgaatttaa cacccatccg acggcgttta tttgcatcaa tatccaggaa
    35221 accgacctcg ctggtcatgc agaagacgtc gcacgttatg ccgaacgttt gcaggtcgtt
    35281 gaccgtaacc ttgcccggct tgttgaggcg atgcagccag atgattgcct ggtcgtgatg
    35341 gcggatcacg gtaacgatcc gaccattggt cacagccacc atacccgtga agtggtgcca
    35401 gtgctggttt atcagcaagg gctggtcgcc acgcagctcg gtgtgcgcac cacgctttct
    35461 gatgtgggag ctaccgtgtg tgaatttttc cgtgcgccac cgccacaaaa tggtcgctct
    35521 tttctttcct ccctccggtt tgcaggagac accctatgag ttttgatccg acgggttaca
    35581 ccctggccca tgagcatctg catattgatc tctccggctt taaaaacaac gtggactgcc
    35641 gccttgatca gtatgcgttc atttgccagg agatgaacga cctgatgacc cggggtgtac
    35701 gtaatgtgat tgagatgacc aaccgttaca tggggcgcaa tgcgcaattt atgctcgatg
    35761 taatgcgcga aaccgggatc aacgtggtgg cctgtactgg ttattaccag gatgcgtttt
    35821 tcccggaaca tgtggtgacc cgcagcgtgc aggaactggc gcaggagatg gtcgatgaaa
    35881 ttgaacaggg tatcgatggc acggagctga aagccgggat catcgcggag atcggctcca
    35941 gcgaaggaaa gattacgccg ctggaagaga aggtatttat tgccgctgcg ctggcgcata
    36001 accagaccgg acgcccgatc tccacgcata cgtcgttcag caccatgggg ctggaacaac
    36061 tggctctgtt gcaagcccac ggagttgatc tttcgcgcgt caccgttggt cactgcgatc
    36121 tgaaagacaa cctcgacaac attttgaaga tgatcgatct cggcgcgtac gtgcagttcg
    36181 acaccattgg taagaacagt tactacccgg acgaaaagcg cattgcgatg ctccacgcgc
    36241 tacgtgaccg tgggttgctg aaccgcgtca tgctgtcgat ggatattacg cgccgctccc
    36301 atttaaaagc caatggtggt tatggctatg actatttatt gaccaccttt attccgcaat
    36361 tgcgccagtc aggattcagt caggccgatg tggatgtgat gttacgtgaa aatccctctc
    36421 aatttttcca ataaggacag actcatgaaa aagattggcg ttgcaggctt acagcgtgag
    36481 cagattaaaa aaaccattga agcgacggct cctggctgtt ttgaagtttt cattcacaac
    36541 gacatggaag cggcaatgaa ggtgaaatcc gggcaactag attattacat tggcgcgtgt
    36601 aataccggtg cgggcgcggc attgtcgatt gccatcgcgg tgattggcta taacaaaagt
    36661 tgcaccattg ccaaaccagg cattaaagcg aaagacgagc atatcgccaa aatgatcgct
    36721 gaagggaaag tggcgtttgg cctttccgtt gagcacgtcg aacacgcgat tccgatgttg
    36781 attaaccatc tgaaataaaa ggcacgacta tggatctgta tattcagatt atcgtggtgg
    36841 cgtgcctgac gggtatgaca tcgcttctgg agcatcgctc ggcggctgtt tttcatgacg
    36901 gcatccaccc gatcctgccg caactgattg aaggctatat gaaccgtcgc gaggcgggga
    36961 gtatcgcttt tggtctgagc attggttttg tggcctcggt ggggatctct tttaccctga
    37021 aaaccgggct gctcaacgca tggttactct ttcttcctac cgatatcctc ggcgtactgg
    37081 cgataaacag cctgatggcg tttggtcttg gcgctatctg gggcgtgttg atccttactt
    37141 gcctgttgcc agttaaccag ctgctgaccg cgctgccggt ggatgtatta ggtagcctcg
    37201 gggaattaag ctcgccggtg gtttctgctt ttgcactgtt cccgctggtg gcgattttct
    37261 accagtttgg ctggaagcaa agtctggtcg ccgccgtggt tgttctgatg acccgtgtgg
    37321 tagtcgtgcg ctatttccca catcttaacc ctgaatccat cgaaatcttt attggcatgg
    37381 tgatgctgct ggggatcgcg ataactcacg acctgcgtca tcgtgatgaa aatgacatcg
    37441 atgccagcgg gctttcggtg tttgaagagc gcacgtcgcg gattatcaaa aacttacctt
    37501 atatcgccat cgtgggagca ttgattgccg ccgttgccag tatgaagatt tttgctggta
    37561 gcgaagtttc gatcttcaca ctggagaaag cgtattccgc aggcgtgacg ccggaacaat
    37621 cgcaaacgct gattaatcag gcggctctgg cagaatttat gcgcggactg gggtttgtgc
    37681 cgttgattgc caccaccgcc ttagcaacgg gtgtgtatgc agttgcgggc tttacctttg
    37741 tttatgcggt gggctatctc tcgccgaatc cgatggttgc agcggtatta ggcgcagtgg
    37801 ttatttcggc ggaagtcttg ctgcttcgtt cgatcggcaa atggctggga cgctacccgt
    37861 cggtgcgtaa tgcgtcggat aacatccgta acgccatgaa tatgctgatg gaagtggcgc
    37921 tgctggtcgg ttcgattttc gcagcaatta agatggcggg ttataccgga ttctctatcg
    37981 cggttgccat ttacttcctc aacgaatccc tgggccgtcc ggtacagaaa atggcggcac
    38041 cggtcgtggc agtaatgatc accggtattc tgctgaatgt tctttactgg cttggcctgt
    38101 tcgttccggc ttaaggaagg cacctatgaa gacgtttcct ttgcaaagcc tgactcttgc
    38161 tgaagcgcag caaaagcagt ttgcgctggt ggacactatt tgccgccatt ttccaggcag
    38221 cgagtttctt gctggcggtg atttagggtt aactccaagt ctgaatcaac cgcgtattac
    38281 ccaacgggtg gagcaggtgc tggctgatgc atttcacgca caggccgcgg cgctggtgca
    38341 gggcgcgggg actggcgcga ttcgcgccgg gctggcggct ttgctcaaac cggggcagcg
    38401 tcttctggtg catgacgcgc ctgtttaccc gacgacacgg gttattattg agcagatggg
    38461 gctgacgctt attactgctg atttcaatga cctgtcggca ctgaagcagg tcgtcgacga
    38521 gcaacaacct gatgccgcgc tggtgcagca tacgcgccag cagccgcagg acagctatgt
    38581 gctggcagat gtgctggcaa cgttgcgcgc ggcaggtgtt ccagcgttaa ccgatgataa
    38641 ctatgcggtg atgaaggtag cgcgaatcgg ctgtgaatgc ggcgcgaatg tctcgacatt
    38701 ctcctgcttc aagctgtttg ggccggaggg cgttggtgca gtggttggcg atgccgatgt
    38761 tattagtcgg attcgcgcca cgctttactc cggcggtagc cagatccagg gctcacaggc
    38821 gctggaagtc ttgcgtggtc tggtgttcgc gccagtgatg catgcggtgc aggcgggagt
    38881 atccgaacgg ttgctggctt tgcttaacgg cggcgcggtg gcggaagtga aaagcgccgt
    38941 cattgccaat gcgcagtcga aggtgttgat tgtcgagttt catcagctga ttgccgccag
    39001 ggtgctggaa gaagcgcaaa agcgtggcgc gttgccttac ccggtcggtg cagagtcgaa
    39061 atatgaaatc ccgccgctct tctatcgact ttcaggaacg tttcgccagg cgaatccaca
    39121 actcgaacat tgtgcgattc gcattaaccc gaatcgcagc ggtgaagaga cggtgctgcg
    39181 gattttgcgt gagagtattg cgagtattta atgctggata gcaccgttcg gcaatcaagc
    39241 taaattccgc actgaacggt cccctcgccc ctttggggag agggttaggg tgaggggaac
    39301 gtgcgggatg taaaattcag tgcgccagca tcgccggatg cgatgctggc gcatcttatc
    39361 cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg gcgcggcaga
    39421 agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg ctgatgcgaa
    39481 acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg gcggcaaaga
    39541 tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga aataactgat
    39601 aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac agcgggatcc
    39661 atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag agatggaaca
    39721 cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg acccgttcct
    39781 gttcgatcac tttctctttc gtcgacttgc cttgtgagac gccaaaatcg gtaaaaccac
    39841 tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct tttccctgcc
    39901 agcggatcag tacgccgtct gccactaagt cgctgatggc tttgcgaatg gtaatgcggc
    39961 tgacgttata ttgtgtacaa agctcgtttt cggtagggat ctgttgcccg gcctggtaaa
    40021 ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg agttgatgag
    40081 agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta ctcggattat
    40141 agggtcgttt tccggcaagt aacagcacgt tggcgtgaag atgctcacgc caacgttata
    40201 cctaccaggc accgtggtac tgaatggttt tcgccgcgca cgccgttccc tgcgccatcg
    40261 cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg aacgaatctc
    40321 cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag aactgcgcgc
    40381 catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct gcgccacggg
    40441 caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg gaggcaaagg
    40501 caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg tcggagaagt
    40561 cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc gcatgtcccc
    40621 agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct tcactcaggg
    40681 caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg tcgtgcagtt
    40741 ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata tcgacgccca
    40801 tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc caggtaatgc
    40861 atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca ttaccgccag
    40921 aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt gtcgccaggg
    40981 ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct ggcgggcata
    41041 gagtctgggc tcgttcatat acatcgtcac cagctccacc gtagagtaac cctcatagcc
    41101 ccgatcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc caggaatgta
    41161 atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgtaatt tatcgcccag
    41221 tttgtcgaaa taactcatca ccggttccgc ctggacatac ggcgcgcaaa tgtcgaccat
    41281 gctgaacagg cgcggcgaag gcaccagcgc cagcgcatga agcacatcat tagcattaca
    41341 cacgacgttc gattcatacg gcgttaacgg ttcgaggatc agatccatgc cgatgttttc
    41401 cgcgtactca cacagttcgc ttaagttctc tgccagccgc ccccagataa cgttaggcgg
    41461 cgtgagatag cccgcgtggg ccgcagaaat cagcgtataa cccgcgttca tctcttttgc
    41521 catatctatc gccagcttga tcatgtccag gctttcacga cgcatatgtt catcgcccag
    41581 catcatgtta tacggatagc cgttggtttc tggcgtgtaa ccgataatcg gcatctgata
    41641 cgtctgcgcc agcgccttga tttgtttgat gccgcccgct tttaagtccg gcgcgaacgc
    41701 gtgcgggcga ccgccccaaa tttcgatgcc gtcgtaaccc agctcgcttg catcacgaaa
    41761 tgcgtgttca ataggcagcc gctggtggcc gcaggtaaac atacctgttt tcatcgggta
    41821 actccttgcg taaatccggc ccgttgccgg gccgggagag ggattaatat tccaccagac
    41881 caccgtaata gcggcgttca tccgggttgt gatctttgta aatagacagg tagtagcaga
    41941 gccactccat tggcacgaac atcaggaacg gtgccagcca cgggtgcagc ccttgcgaaa
    42001 tttcggcgta atcgatgacg atcacgttgt cagtacgctg tttaacaaag ttaatggcgc
    42061 gttcggtggt gtggcgactt tcatcattgc cgagcaggaa caggaacgga acgcccggtt
    42121 cgacaatctc cagcgggcca tggcggaact ctccgctctc aatcacgcag ccgtgcgtcc
    42181 aggtaaattc catcagcgtt acaatgcctt ctttgtaacc cagcggacgc agcggacccg
    42241 cagcaacggt ataaatcatc ggccactggc tggccagttc accaagctgg cggccttttt
    42301 cttcccaggt gcgtaccaga tgaccgagcg cattcggcaa ctgcttgaga tcgtttttga
    42361 tcttgccgat ttccgcgttc ggcgcgaggc gggtgatcat ctccagcacc acgctgtagc
    42421 agagcagcag gtgaatttcc cagatacagt cggcctgata atcaatgcta aattccgccg
    42481 ccgaggtaat cgggctgtcc gcgcgtttgg tgaacgccgc agtgagtgct ccgcaggccc
    42541 ggcccagctc cagcgctttg attacctctt ccgttttacc gtagtcagaa acgccaatca
    42601 ccgcgcaacg atcgtcgagg cgatacgggg tgttatcgca gaactcccag ccggaaatgg
    42661 cgtagacctg aagatcggaa aagcgatccg ccagatgttt cgccgtttgc gccgcgttga
    42721 gtggcgagcc gcaggcaacg aaataaatac gatcaatgtc gcgcttcacc atctcttcca
    42781 cgatggcgtg caccagcgga acgtcatggc tgagaacttt ttccacttcc tgaaccatat
    42841 tttcggtgac cagaaagtcc acggtgcttt tatcaatatc caacatttta ttttcttcag
    42901 ataacattac gacaaagcgt tgagctggcg gctacgcttc gcccagaagg cgtaagcagg
    42961 cagaccagta gcaataacga tggcggcgca gataaggccg ggaatcggtg cccagacaaa
    43021 ggttgaggcg accagaatga ggcttgacgc aatggcgagg gtggtcatca gcccgaaagc
    43081 cggagtacgc cacagcggtt tgtaatcgtc gcgtttacga caccagatga tggagccgaa
    43141 ggtgagggta tttttgaaac acatcaccag ggtgaaataa cccagcaggc tggtgagatc
    43201 ggaaacgaag atgaagaaga tccccagcgc cccttgcagg atgatggaga catccggcgt
    43261 gttgtatttc ggatgcgcat ggccgaagca tttaaagaac aggttgtctt tcgccatcgc
    43321 gtattccagc cgcggctggt acatcacgca gctggaaagc gaaccaagaa tgacgatcat
    43381 cgccgtgatg gcaacaaaga gcccagcggt gctgccgagt gcggggatct aggtcagggc
    43441 gtcggaaatc ggcgtttcag aattggcgag tttgtcgaag ggcatcaggc cggaaatcac
    43501 cagcgccagc agggtgtaga gcaccagaac cagcagacag gaaccaatca gcgctcgtgg
    43561 catggttttt ccggggtttt taatttcgcc ggtcatataa cagatagagg ccatgccggt
    43621 atacgaccaa ctggtggcag agatccccgc cagcagcgcc ataaagctgc ccgttgcgcc
    43681 aatcgcagtg gtggtaaggg cggcaaaatt ctccgcttta aaccagaaga tcccaaggcc
    43741 aatgacgata gtgaacggga taattttggc gatggtaatt agcgtctgaa acgctgcgcc
    43801 gccttcaacg gagcgcaggt gtagcaacat aaaggcgata attaatccgg cggcgataaa
    43861 tttaccgagc aacggatcga taggcgttaa aaagccaaga ttgctgacaa tcgccagcgc
    43921 cataatcgac aatgacggcg catcgttggc ccagaagctg gcccagccgg agaggaaagc
    43981 cagcggtcgg cttccggcat ttttcagata aacataatct gcgccatttt ccggataagc
    44041 ggtggatagt tccgcataga cgcacatttg cgggatcaca attaacccgc caatgacaaa
    44101 cgcgagcacc gtaagccacg gcgtgcccgc tgcttttgcc acttcaccca cagatacaaa
    44161 aataccggag ccgacggttg tcccgacggc gattgcaaga acggcccaaa atccgagctt
    44221 gcgttggagt tcctggcttc ccatagcgat acctttttct tgtccaatac gttgtagggg
    44281 agagtttgca gccatttaaa aggtaacatg acaatgcatg atgaatataa caacatatta
    44341 tgttatgtga tgtggaggaa gatcacaaaa tttcgcacag gatcgcgctg tggctaatgg
    44401 atgtagttat aaaattgaat ttaaagtgaa aatattttta cgggcggggg caagaaggac
    44461 atagaaacaa atacgccctc ggaaaatcca gagggcgtcg ggcaattaaa ccggtgttag
    44521 ccgatttctg tcagagactt actgtgcagt aggaccgcaa ccgccaatga ttttagaaat
    44581 agaaattgcc gggtgcagca ggtagtcgta agagcagttc ttatcacggt tttcaatatg
    44641 accagtacat gcagttaacg tagccagtac tgcacctacc agtgcaactt taataaattt
    44701 gttca
//
LOCUS       DAZOZX010000020        43406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZOZX010000020 DAZOZX010000000
VERSION     DAZOZX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.020"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            118..567
                     /gene="alaE"
                     /locus_tag="ACHUPT_001053"
     CDS             118..567
                     /gene="alaE"
                     /locus_tag="ACHUPT_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0818719.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(604..948)
                     /locus_tag="ACHUPT_001054"
     CDS             complement(604..948)
                     /locus_tag="ACHUPT_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0818720.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1100..1429
                     /locus_tag="ACHUPT_001055"
                     /pseudo
     CDS             1100..1429
                     /locus_tag="ACHUPT_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            1677..1922
                     /gene="nrdH"
                     /locus_tag="ACHUPT_001056"
     CDS             1677..1922
                     /gene="nrdH"
                     /locus_tag="ACHUPT_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0818721.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1919..2329
                     /gene="nrdI"
                     /locus_tag="ACHUPT_001057"
     CDS             1919..2329
                     /gene="nrdI"
                     /locus_tag="ACHUPT_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR0818722.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2302..4446
                     /gene="nrdE"
                     /locus_tag="ACHUPT_001058"
     CDS             2302..4446
                     /gene="nrdE"
                     /locus_tag="ACHUPT_001058"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0818723.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4456..5415
                     /gene="nrdF"
                     /locus_tag="ACHUPT_001059"
     CDS             4456..5415
                     /gene="nrdF"
                     /locus_tag="ACHUPT_001059"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0818724.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5770..6972
                     /gene="proV"
                     /locus_tag="ACHUPT_001060"
     CDS             5770..6972
                     /gene="proV"
                     /locus_tag="ACHUPT_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0818725.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6965..8029
                     /gene="proW"
                     /locus_tag="ACHUPT_001061"
     CDS             6965..8029
                     /gene="proW"
                     /locus_tag="ACHUPT_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0818726.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8087..9079
                     /gene="proX"
                     /locus_tag="ACHUPT_001062"
     CDS             8087..9079
                     /gene="proX"
                     /locus_tag="ACHUPT_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0818727.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9271..10450
                     /locus_tag="ACHUPT_001063"
                     /pseudo
     CDS             9271..10450
                     /locus_tag="ACHUPT_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            10574..11311
                     /gene="ygaZ"
                     /locus_tag="ACHUPT_001064"
     CDS             10574..11311
                     /gene="ygaZ"
                     /locus_tag="ACHUPT_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0818728.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11301..11636
                     /gene="ygaH"
                     /locus_tag="ACHUPT_001065"
     CDS             11301..11636
                     /gene="ygaH"
                     /locus_tag="ACHUPT_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0818729.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11727..12257
                     /gene="emrR"
                     /locus_tag="ACHUPT_001066"
     CDS             11727..12257
                     /gene="emrR"
                     /locus_tag="ACHUPT_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0818730.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12384..13556
                     /gene="emrA"
                     /locus_tag="ACHUPT_001067"
                     /pseudo
     CDS             12384..13556
                     /gene="emrA"
                     /locus_tag="ACHUPT_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            13573..15111
                     /gene="emrB"
                     /locus_tag="ACHUPT_001068"
     CDS             13573..15111
                     /gene="emrB"
                     /locus_tag="ACHUPT_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0818731.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15175..15690)
                     /gene="luxS"
                     /locus_tag="ACHUPT_001069"
     CDS             complement(15175..15690)
                     /gene="luxS"
                     /locus_tag="ACHUPT_001069"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0818732.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15840..17396)
                     /gene="gshA"
                     /locus_tag="ACHUPT_001070"
     CDS             complement(15840..17396)
                     /gene="gshA"
                     /locus_tag="ACHUPT_001070"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0818733.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17469..17897)
                     /locus_tag="ACHUPT_001071"
     CDS             complement(17469..17897)
                     /locus_tag="ACHUPT_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0818734.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17894..18460)
                     /gene="yqaB"
                     /locus_tag="ACHUPT_001072"
     CDS             complement(17894..18460)
                     /gene="yqaB"
                     /locus_tag="ACHUPT_001072"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0818735.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18608..18684)
                     /locus_tag="ACHUPT_001073"
     tRNA            complement(18608..18684)
                     /locus_tag="ACHUPT_001073"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18648..18650),aa:Arg,seq:acg)
     gene            complement(18749..18825)
                     /locus_tag="ACHUPT_001074"
     tRNA            complement(18749..18825)
                     /locus_tag="ACHUPT_001074"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18789..18791),aa:Arg,seq:acg)
     gene            complement(18829..18921)
                     /locus_tag="ACHUPT_001075"
     tRNA            complement(18829..18921)
                     /locus_tag="ACHUPT_001075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18885..18887),aa:Ser,seq:gct)
     gene            complement(19237..19422)
                     /gene="csrA"
                     /locus_tag="ACHUPT_001076"
     CDS             complement(19237..19422)
                     /gene="csrA"
                     /locus_tag="ACHUPT_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0818736.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(19657..22287)
                     /gene="alaS"
                     /locus_tag="ACHUPT_001077"
     CDS             complement(19657..22287)
                     /gene="alaS"
                     /locus_tag="ACHUPT_001077"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0818737.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(22415..22915)
                     /gene="recX"
                     /locus_tag="ACHUPT_001078"
     CDS             complement(22415..22915)
                     /gene="recX"
                     /locus_tag="ACHUPT_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0818738.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23158..24219)
                     /gene="recA"
                     /locus_tag="ACHUPT_001079"
     CDS             complement(23158..24219)
                     /gene="recA"
                     /locus_tag="ACHUPT_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0818739.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(24299..24796)
                     /gene="pncC"
                     /locus_tag="ACHUPT_001080"
     CDS             complement(24299..24796)
                     /gene="pncC"
                     /locus_tag="ACHUPT_001080"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0818740.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(24941..26026)
                     /gene="mltB"
                     /locus_tag="ACHUPT_001081"
     CDS             complement(24941..26026)
                     /gene="mltB"
                     /locus_tag="ACHUPT_001081"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0818741.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            26282..26844
                     /gene="srlA"
                     /locus_tag="ACHUPT_001082"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             26282..26844
                     /gene="srlA"
                     /locus_tag="ACHUPT_001082"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            26841..27800
                     /gene="srlE"
                     /locus_tag="ACHUPT_001083"
                     /gene_synonym="gutE"
     CDS             26841..27800
                     /gene="srlE"
                     /locus_tag="ACHUPT_001083"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0818742.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            27811..28182
                     /gene="srlB"
                     /locus_tag="ACHUPT_001084"
                     /gene_synonym="gutB"
     CDS             27811..28182
                     /gene="srlB"
                     /locus_tag="ACHUPT_001084"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0818743.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            28186..28965
                     /gene="srlD"
                     /locus_tag="ACHUPT_001085"
     CDS             28186..28965
                     /gene="srlD"
                     /locus_tag="ACHUPT_001085"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR0818744.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            29070..29429
                     /gene="gutM"
                     /locus_tag="ACHUPT_001086"
     CDS             29070..29429
                     /gene="gutM"
                     /locus_tag="ACHUPT_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0818745.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            29496..30269
                     /gene="srlR"
                     /locus_tag="ACHUPT_001087"
                     /gene_synonym="gutR"
     CDS             29496..30269
                     /gene="srlR"
                     /locus_tag="ACHUPT_001087"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0818746.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            30262..31227
                     /gene="gutQ"
                     /locus_tag="ACHUPT_001088"
     CDS             30262..31227
                     /gene="gutQ"
                     /locus_tag="ACHUPT_001088"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0818747.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31224..32738)
                     /gene="norR"
                     /locus_tag="ACHUPT_001089"
     CDS             complement(31224..32738)
                     /gene="norR"
                     /locus_tag="ACHUPT_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0818748.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            32925..34364
                     /gene="norV"
                     /locus_tag="ACHUPT_001090"
     CDS             32925..34364
                     /gene="norV"
                     /locus_tag="ACHUPT_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0818749.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            34361..35494
                     /gene="norW"
                     /locus_tag="ACHUPT_001091"
     CDS             34361..35494
                     /gene="norW"
                     /locus_tag="ACHUPT_001091"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0818750.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(35622..37874)
                     /gene="hypF"
                     /locus_tag="ACHUPT_001092"
     CDS             complement(35622..37874)
                     /gene="hypF"
                     /locus_tag="ACHUPT_001092"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0818751.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38027..38554)
                     /gene="hydN"
                     /locus_tag="ACHUPT_001093"
     CDS             complement(38027..38554)
                     /gene="hydN"
                     /locus_tag="ACHUPT_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0818752.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(38703..39713)
                     /gene="ascG"
                     /locus_tag="ACHUPT_001094"
     CDS             complement(38703..39713)
                     /gene="ascG"
                     /locus_tag="ACHUPT_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0818753.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(39775..39846)
                     /locus_tag="ACHUPT_001095"
                     /pseudo
     CDS             complement(39775..39846)
                     /locus_tag="ACHUPT_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39856..41565)
                     /gene="hycE"
                     /locus_tag="ACHUPT_001096"
     CDS             complement(39856..41565)
                     /gene="hycE"
                     /locus_tag="ACHUPT_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0818754.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(41583..42506)
                     /gene="hycD"
                     /locus_tag="ACHUPT_001097"
     CDS             complement(41583..42506)
                     /gene="hycD"
                     /locus_tag="ACHUPT_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0818755.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(42509..>43406)
                     /locus_tag="ACHUPT_001098"
     CDS             complement(42509..>43406)
                     /locus_tag="ACHUPT_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR0818756.1"
                     /translation="ALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRD
                     IEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLL
                     GPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCV
                     IGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPS
                     RSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSAL
                     LFRRMALVELAVLVVIIVSRGA"
BASE COUNT        10261 a        11790 c        11080 g        10275 t
ORIGIN      
        1 cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga tggcaatgag
       61 atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg
      121 ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac
      181 tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct
      241 ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg
      301 taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat
      361 ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta
      421 gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg
      481 atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc
      541 agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc gccagtcgcg
      601 tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact
      661 aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg
      721 ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc
      781 ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata
      841 gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa
      901 accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg
      961 ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt
     1021 ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg
     1081 aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt
     1141 gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattgaagtc
     1201 ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcgttgct
     1261 gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cgcgcgatgc
     1321 cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc
     1381 tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga
     1441 taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt gcctggtgaa
     1501 tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat cactatttgc
     1561 tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga
     1621 gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc
     1681 gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg
     1741 aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcagcggaag
     1801 cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt
     1861 ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat
     1921 gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta tcgaacgttt
     1981 aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc
     2041 ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca
     2101 ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc
     2161 tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg
     2221 cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt
     2281 tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg
     2341 atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg tcgcattcag
     2401 ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt
     2461 gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat
     2521 gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac
     2581 accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg
     2641 ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg
     2701 gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga tgaaatgctg
     2761 tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc
     2821 gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg
     2881 gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat
     2941 ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg
     3001 gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc gcgtcagggg
     3061 gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa
     3121 cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg
     3181 gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccctatgac
     3241 gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca ctatgacgaa
     3301 ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga
     3361 ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga cacagtaaac
     3421 cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag
     3481 gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg ccatgatatt
     3541 tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga tttttcccgc
     3601 acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca tatccgcagc
     3661 gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg gcagatgaat
     3721 ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc cctggatttc
     3781 accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggcg
     3841 cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat
     3901 ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg cgaactgttt
     3961 gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg
     4021 atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg gtctatctct
     4081 tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa
     4141 gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga aaatctggcg
     4201 ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta cgcggaagcg
     4261 actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac cgccaccact
     4321 cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac
     4381 atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca
     4441 ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct
     4501 gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag
     4561 gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga acaacaactg
     4621 acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg
     4681 ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc gaatatcagc
     4741 tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgccagacc
     4801 aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct
     4861 cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg
     4921 tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc
     4981 ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga agcagtccac
     5041 ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt
     5101 gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa
     5161 tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt
     5221 tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg
     5281 gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat
     5341 ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa
     5401 gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat
     5461 tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt
     5521 tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat
     5581 atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg cgatttgctc
     5641 gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg
     5701 gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat aaagaaatca
     5761 ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt tggtgagcat
     5821 ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa
     5881 actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt
     5941 gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg
     6001 attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgac
     6061 gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta
     6121 atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat
     6181 gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat
     6241 gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc
     6301 gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca
     6361 ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc
     6421 attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg aattgccatt
     6481 atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg
     6541 aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt cagtgcgaaa
     6601 gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt cggcccacgt
     6661 tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt
     6721 aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa
     6781 ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc
     6841 gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa
     6901 cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta
     6961 aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca
     7021 atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg gtgctgactg
     7081 gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa
     7141 aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt
     7201 ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca
     7261 attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca tcacctggca
     7321 gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca tcggcgcaat
     7381 cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc tgctgttctg
     7441 tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag cggcgaaaat
     7501 tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat
     7561 cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgcgct
     7621 gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga
     7681 agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct
     7741 ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt
     7801 ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg
     7861 tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat
     7921 cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg
     7981 gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg
     8041 cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt
     8101 actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc
     8161 gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa ccttccagac
     8221 gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac ccagcgaagt
     8281 ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa
     8341 ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga aattttatcg
     8401 tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga aaaccgccga
     8461 ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga
     8521 taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg
     8581 tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc ataatcaggg
     8641 gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt
     8701 ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga aagatgtggt
     8761 ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata ccaaactgcc
     8821 gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca acaaagcctg
     8881 gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc cagtggccga
     8941 tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg
     9001 ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc
     9061 gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc tcatccgccg
     9121 ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca aaccacatcg
     9181 cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt attgatttcc
     9241 agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga gcttagcccg
     9301 gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa ctattacgcc
     9361 cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt
     9421 attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt tcccctcggt
     9481 gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc gggcggtatg
     9541 ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc attaaccggt
     9601 ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc ttcaccggat
     9661 aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat attgctggca
     9721 cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgct
     9781 tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca aatgaaatca
     9841 gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt catcagcgat
     9901 aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt cagcattctc
     9961 tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga tggtgacatt
    10021 ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc gggcggtttt
    10081 gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac tttcatggct
    10141 ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc tggtgctgga
    10201 cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa tacatcctga
    10261 tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg gtgccgccgg
    10321 ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt gtctggctgg
    10381 cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc gtcaggaagc
    10441 cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg tgttaagaag
    10501 cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt aacaatcgtt
    10561 aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg cgaccttcat
    10621 ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt
    10681 cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat
    10741 catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt
    10801 gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt atggcccgtc
    10861 actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat gggcgtttgg
    10921 cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata atcgccgctg
    10981 gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac
    11041 ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc
    11101 attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa
    11161 acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa cgctattttc
    11221 tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc
    11281 attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg
    11341 cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa
    11401 ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc
    11461 tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc
    11521 tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa
    11581 cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca
    11641 caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt tactatatcg
    11701 gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc
    11761 taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc
    11821 tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg
    11881 ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta
    11941 ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg
    12001 ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac gatcgccgct
    12061 gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc
    12121 agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagctcg
    12181 agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc
    12241 tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat
    12301 gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag
    12361 ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac
    12421 cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa
    12481 ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg
    12541 atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct ggcagcgtga
    12601 cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg gtcactctcg
    12661 acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc
    12721 gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt gaggtgcaaa
    12781 aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca
    12841 acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc
    12901 aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac
    12961 tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc tagctggcgc
    13021 tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt gccgtgcaac
    13081 cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt
    13141 gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt cagccggtca
    13201 ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg
    13261 atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc ggtaactgga
    13321 tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag ctggagcaat
    13381 acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc
    13441 aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg cgtgaaatca
    13501 gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca
    13561 gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg
    13621 attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc taacgtggcg
    13681 atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg ggtaatcact
    13741 tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc
    13801 ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc ttgggcgtgt
    13861 ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg gattgtcgcc
    13921 gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc agccaaacgc
    13981 tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccgatc
    14041 ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat caacgtgccg
    14101 attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga aacccgcacc
    14161 gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat cggcagcctg
    14221 cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga aattatcatt
    14281 cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat
    14341 aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt
    14401 atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca gttgttgcag
    14461 gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt
    14521 ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg
    14581 gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa
    14641 ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggtc
    14701 tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg
    14761 gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg cacgtcgata
    14821 accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac tgagtcggta
    14881 aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg
    14941 caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct gattatttcc
    15001 gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg gctggtgtgg
    15061 tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta agtacaacta
    15121 agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactagat
    15181 gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt tgatgcgtac
    15241 ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta
    15301 agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca ctttcagcac
    15361 gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag
    15421 actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt
    15481 accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat ggatccctct
    15541 ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc
    15601 gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg
    15661 atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat
    15721 tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca
    15781 tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt tcttttctgt
    15841 caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc catttcctgc
    15901 tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccagc
    15961 ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt tccgccaata
    16021 ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag
    16081 caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat gctatccagc
    16141 gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag taacgggaac
    16201 tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc aaggatcacc
    16261 cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc
    16321 agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc
    16381 ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac
    16441 agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac
    16501 agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca
    16561 ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg
    16621 tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg
    16681 agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc
    16741 tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca
    16801 ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag
    16861 cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt cgcttgccag
    16921 aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg
    16981 tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg
    17041 tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac
    17101 atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcagc
    17161 atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg
    17221 gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc
    17281 agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt
    17341 aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg
    17401 acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag
    17461 gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac tgcaacatag
    17521 cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat gcgcatccat
    17581 cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact
    17641 gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt tttacgcaat
    17701 ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct
    17761 gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac cagcacgact
    17821 tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc
    17881 gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct gcacgggccg
    17941 cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac
    18001 gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag
    18061 cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg
    18121 cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt
    18181 caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt
    18241 cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt gcctgagcaa
    18301 tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac tgaagaccgt
    18361 agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc aggattgtgc
    18421 catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa
    18481 aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa
    18541 attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg aaattttttg
    18601 gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac
    18661 tctatccagc tgagctacgg atacatcggg aaacttactt tactgcagat tttttgatac
    18721 cgctactaaa gccgtatcaa gtaagagatg gtgcatccgg gaggattcga acctccgacc
    18781 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg gcggtgaggc
    18841 ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg ggagcgcctt
    18901 cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg
    18961 ctcatcatcg ctgcgtggcg cacatattac tttctgagac ttataagtca aacaattttt
    19021 cccacacttt tatcgtttgc acacttcacg ctcaattagt ctgtaaaagc gtcaaaaaga
    19081 gtgttttatc tacagaaaaa cggaggtctg gcagatagca gtaatgcaaa aaaatggaga
    19141 cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag aaattttgag
    19201 ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact gctgggattt
    19261 ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct tcggggcatt
    19321 tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga cctcatcccc
    19381 aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct ccttgaaaga
    19441 ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc caaaaagtaa
    19501 gcaatgacaa acacattaca tgtaagcagt catggcatta cattctgtta aacctaagtt
    19561 tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta atgcgaatgc
    19621 cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg cgctgaccca
    19681 gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc
    19741 catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac caatcagttc
    19801 ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag aaaccttacc
    19861 ttcggctacc gttgccagca cgataattgt cgaccccagc tgatttttta aatcgtcaac
    19921 catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca gcttaacacc
    19981 attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt
    20041 aagctgttgt agttcttttt ctaactgacg cgtacgttcc agtactgagc gcactttatc
    20101 cgccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc gatcgctgtc
    20161 tgcatgaacg gtggtgatag cgccttctcc ggttaccgct tcgatacgac gaacgcctgc
    20221 agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg
    20281 agtaccgcca cacagctcgg tggagaaatc gcccatgctc agcacgcgta cacgctcatc
    20341 atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga gattcatgat
    20401 gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt cgaccgcacg
    20461 aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt
    20521 aaccagtgaa cctttctgcg atacatgagt ccccagaacc tggcgcagcg cagcgtgcat
    20581 caggtgcgtt gcggagtgat tcagacgaat acgggcgcga cgagcctcat caatatcagc
    20641 ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc
    20701 ctggccgtat ttctgcgtat cttccaccgc gaaggagaag ttagcgcctt tcagttcgcc
    20761 tttatcacca acctgaccgc cggattccgc atagaatggc gtttgatcca gcacgaccac
    20821 agcttcctgg ccggcattga tggcatcaac cgctttacca tcaacaaaca gcgcggtcac
    20881 tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactat caacacggat
    20941 cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcgcgacgac gctgctcttc
    21001 cattgctgct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac aaacatcagc
    21061 cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagccgttt caccatccag
    21121 cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc gctccagagt
    21181 acgggcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct gctggcgttt
    21241 caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa
    21301 ggtttctttc gcgccgagca tattgccgtg acgcactgcg cgacgaatga tacgacgcag
    21361 tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga acgcacaaga
    21421 acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggtcg cgccagtgac
    21481 tttcgctacc gcctggatca acgtgcggaa caggtcgatg tcatagttag agttaacgtg
    21541 ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg gcttcggcag
    21601 cggttccatc gtgccatctg cctggcggtt gaactgcatg aagacgatgt tccagatctc
    21661 aatgtagcgg tcgccgtctt cttccggact tcccggaggg ccaccccaaa tgtggtcgcc
    21721 gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca tctgccagaa
    21781 gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac gttcgcgcgg
    21841 gatcccgact tctttttccc agatttcgta ggcttcgtcg tcgctttcat agacggtaac
    21901 ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa
    21961 ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa
    22021 ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg
    22081 cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc caaggaacac
    22141 atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt tatggggtac
    22201 cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg
    22261 acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac gaaatatcgc
    22321 caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga ataagataag
    22381 ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt cgccaaatat
    22441 cctggatatc ttccatcaga tagccacgat agagcagaaa acgctggatc ttaacttttt
    22501 ctgaaaagac agttggcaaa ggttcaccat attttcgcgt cgcctgatcg cgcgccagtg
    22561 cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt
    22621 tctgattcag ttcctgacga atacgtgcag gtccataccc tttgcggcta cggctggcga
    22681 taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac caggcaataa
    22741 cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg cccataatcg
    22801 gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc agaatgcgta
    22861 ccgcacgatc caacaggcga gcatatgccg ggcgacggga tgttgattct gtcatggcat
    22921 atccttacaa cttaaaaaag caaaagggcc gcagatgcgc cccaggtttt gctgataaac
    22981 agatttaata gtcaatgagt atgcagaatg ttcccgcagg cccaattcgt tagaggatcg
    23041 aggtgagagt aatttttcac gccagtgctg gcctgttccc ctcaccctaa ccctctcccc
    23101 aaaggggcga ggggaccgtc cgtgcacaca ttatctatta tcatcaataa cgaacgatta
    23161 aaaatcttcg ttagtttctg ctactccttc gctgtcatct acagagaaat ccggcgttga
    23221 gttcgggttg ctcagcagca actcacgtac tttcttctcg atctctttcg cggtttccgg
    23281 gttatctttc agccaggcag ttgcattcgc tttaccctga ccgatcttct cacctttgta
    23341 gctgtaccac gcgcctgctt tctcgatcag cttctctttt acgcccaggt caaccagttc
    23401 gccgtagaag ttgatacctt cgccgtagag gatctgaaat tcagcctgtt taaacggtgc
    23461 agcgattttg ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc
    23521 tttcaccgcg ccgatacgac ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt
    23581 accaccggta gtggtttccg ggttaccgaa catcacacca attttcatac ggatctggtt
    23641 gatgaagatc agcagcgtgt tggactgctt caggttaccc gccagcttac gcatcgcctg
    23701 gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt cgatttccgc
    23761 tttcggcgtc agtgccgcca cggagtcaac gacgataacg tctactgcgc cagaacgcgc
    23821 cagggcgtca cagatttcca gtgcctgctc gccggtatcc ggctgggagc acagcaggtt
    23881 atcgatatcg acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat
    23941 aaacgcacag gttttacctt cacgctgcgc tgcggcgatc acctgcagcg tcagcgtggt
    24001 tttaccggaa gattccggtc cgtagatttc gacgatacgg cccatcggca gaccacctgc
    24061 cccaagcgcg atatccagtg aaagcgaacc ggtagagatg gtttccacat ccatggaacg
    24121 gtcttcaccc aggcgcatga tggagccttt accaaattgt ttctcaatct ggcccagtgc
    24181 tgccgccaac gctttctgtt tgttttcgtc gatagccatt tttactcctg tcatgccggg
    24241 taataccgga tagtcaatat gttctgttga aacaattata ctgtatgctc atacagtatc
    24301 aagtgttttg tagaaattgt tgccacaagg tctgcaatgc atacgcagta gcctgacgac
    24361 gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt aatgccttca ccgcgggcag
    24421 tggcaaaagc aaaccagacg gtgccgacag gcttctcttc actgccgcca tccggcccgg
    24481 cgataccact aatagacacg gcataatcag cacgagccgc tttcagtgca cctatcgcca
    24541 tttccaccac gacgggttca ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacgc
    24601 cgatcatctg cgctttggct tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg
    24661 agctaccggc aatatcggta atcactttcg ctacccaacc accggtacaa gactcggcag
    24721 ttgttacggt tgcgccacgg gctttcagcg cctgcccaac ctgttcgctt aactgcatca
    24781 gttcactgtc agtcatcaca acctctgtca gtcaaaaatt tatgccggac aagatagcac
    24841 tttcgtcagg aagatggtgt tggcgttgca attttacgag gaggattcag aaaaaagctg
    24901 attagccaga gggaagctca cgcccccctc ttgtaaatag ctactgtact cgcgccagcg
    24961 ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg ggtgctgtgg ttgtaacggg
    25021 tgatggtgta gaagttcggc agaccgtacc agtactggta gccggtgcca acatccagac
    25081 gcagcaggct ggcttgttga tggttgccca gcggctgctg tggcgttaaa cctgcggcgg
    25141 caagctgcga aatgctgtac ttagttttga aaccatttgg caagcctgga gcctgaccgt
    25201 ttgccattat cgcgacctga tcgcctttca cccagccgtg cgctttgaaa tagttcgcca
    25261 cgctaccgat cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta
    25321 ccgcatattg tttgtaagac gacggcataa actgtccgta gcccatcgcc ccggcaaagg
    25381 aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc catcagcagg aaggtttcca
    25441 gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat
    25501 cgaggatgcg agttttcccc atcacgcgcc cccagcgggt ttcaacgccg ataatcccga
    25561 cgataatttc cggcggtacg ccatacacct gccacgcgcg attcaacgca tcttcatact
    25621 gattccagaa aaccacaccg ttctgcacgt tgtccggcgt aataaatttt ttgcgataac
    25681 gcagccatgc gccgttcgga cctgatggcg gtttcaccga tgtggttggt gcctggttat
    25741 ccatcagccg cagtaccgaa tccagacgct tcgcctggga gagaatttcc tgcaactgct
    25801 gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa ctgctgggca ttcgggttat
    25861 tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag
    25921 acggcgttcc ggtggtcgtt tcagtctcag taggttttgg cttgctgcta caggcggcaa
    25981 gcaacacaaa aaggggaagc aatgttacat aacgacgctt gaacatgagg ggtccattta
    26041 acagattcaa ccaggggcaa gtatggtaaa gcatcacacc ccgcacaagg aagcggtagt
    26101 cactgcccga tacggacttt acataactca actcattccc ctcgctatac ttttattcaa
    26161 actttcaaat taaaatattt atctttcatt ttacgatcaa aataacactt ttaaaccttt
    26221 caacctgatt agattaggtt gccgtttggt aataaaacaa taaatcctga aggagagaac
    26281 aatgatagaa accattactc atggtgcaga gtggtttatc gggctgttcc aaaagggcgg
    26341 agaggtgttt accgggatgg tgaccggcat tcttccgctg ttgattagcc tgcaggttat
    26401 catgaacgca ctgattaatt ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg
    26461 cgccggtaac cctgtttccc gttacctact gttaccgtgc attggcacgt ttgtcttttg
    26521 caatccgatg accttaagcc tgggtcgctt tatgccggaa aagtacaaac ccagctacta
    26581 cgcggcggcc tcttatagct gccactcaat gaatggcctc ttcccccata tcaaccctgc
    26641 gaactgtttg tttatcttgg cattgccagc ggtctgacaa cgctgaacct gccacttggc
    26701 ccactggcgg tgagttatct gctggttggt ctggtcacca atttcttccg cggctgggtg
    26761 accgatctga ccaccgccat ttttgagaaa aagatgggca ttcaacttga acaaaaagtt
    26821 cacctggcag gagcaacatc atgacgcgta ttcggatcga aaaaggaacg ggtggctggg
    26881 gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat cgtctatatc accgccggta
    26941 cccggcctgc gattgttgac aaactggcac aacttactgg ctggcaggct attgacggat
    27001 ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac tgtggcggca
    27061 cattacgctg cggcatctat ccgaaacgac gtattcccac cattaatatc cactcgacgg
    27121 gcaagtccgg tccgctggcg cagtacattg tggaagatat ttatgtctct ggcgtaaaag
    27181 aagaaaacat cactgtagtg ggtgatgcga caccacaacc ctcttccgtg ggccgtgact
    27241 atgacaccag taagaaaatc accgaacaaa gcgatggttt actggcgaag gtgggaatgg
    27301 gcatggggtc cgccgttgcc gtgttgtttc agtctggtcg tgacaccatc gacactgtat
    27361 taaaaaccat tctgccgttt atggcattcg tctcggcgct cattggcatc attatggctt
    27421 ctggccttgg tgactggatt gcccacggtc ttgctccgct agcgagccat ccactgggtc
    27481 tggtcatgct ggcgctcatc tgctccttcc cactgctttc acctttcctc ggcccaggcg
    27541 cagttatcgc acaggttatc ggcgtattga ttggcgtgca gattggtctc ggcaatattc
    27601 cgccgcatct ggctttaccg gcactgtttg ccatcaacgc gcaggcggct tgcgacttca
    27661 tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac ggttcgcgtc ggtgtccctt
    27721 ctgtactggt gagccgcttt ttaaccggcg cgccgactgt actgatcgcc tggtttgtct
    27781 ccggttttat ctatcaatag aggctgaaac atgaccgtta tttatcagac caccatcacc
    27841 cgtatcggcg cgagtgccac tgacgccctc agcgaccaga tgctcatcac ctttcgtgaa
    27901 ggcgcgccag cggacctcga agagtattgc ttcattcatt gccacggcga gttgaaaggt
    27961 gcactccatc ccggtttgca attttcactc gggcagcatc gctatccggt gaccgctgtt
    28021 ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg cttcgatggt
    28081 ttaaacgaag cggaatttcc gggcactgtc catgtggcag gccctgtacc cgacgatatc
    28141 gcgccgggat cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa tcaggttgcc
    28201 gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt gccacggtct ggctgccgag
    28261 gggtatcgcg tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt ggcacaagaa
    28321 attaacgccg aatatggtga aggtatggcg tacggttttg gtgctgacgc cactagcgag
    28381 caaagcgttc tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt ggatttgctg
    28441 gtctatagcg ccggaatagc aaaagcagcc tttatcagcg acttccagct cggcgatttt
    28501 gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt gtgcccgtga attttcgcgt
    28561 ttgatgatcc gcgacgggat tcagggtcgc attattcaga tcaactcgaa atccggcaaa
    28621 gtgggcagca aacacaactc tggctacagc gcagcgaaat ttggtggcgt cgggctgact
    28681 caatcactgg cgctggatct ggcggagtac ggcattacgg tgcattcact gatgctcggt
    28741 aacctgctga aatcgcggat gttccagtca ctgttgccac aatacgcgac caagctgggt
    28801 atcaaaccgg atcaagtcga gcagtattac atcgacaaag taccgctcaa acgcggctgc
    28861 gattatcaag atgtgctgaa tatgctgctg ttctacgcca gtcctaaggc gtcgtactgc
    28921 accggacagt cgatcaatgt caccggcggt caggtgatgt tctgatcaac agcggagatc
    28981 cgttaaggat ctccgtgaga ctatagaatg cctgatgcgc tacgctcatc aggcatacag
    29041 gacttccgcc actacattaa ggaaaagtta tggtatccgc actcatcacc gtcgccgtta
    29101 tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat ttctcgtttt aaccgtgcct
    29161 tcgacacact atgccagcaa gggcgggttg gcgtggaccg ttccagcggg cgctttaaac
    29221 cgcgggtcgt ggtcgccatc gcgctggacg atcagcagtg catcgtcgac accttgttta
    29281 tgaaaggact gactgtcttc gcccgaccgc aaaaaattcc cgcaattacc ggcatgcatg
    29341 cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
    29401 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt
    29461 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg
    29521 ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg gcgcaatact
    29581 ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat gccggaaccg
    29641 tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg cctatcgatc
    29701 ataaaacgct catcaacacc cacaagaaag agctgattgc agaagctgcc gttagtttta
    29761 tccatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag attgttcccc
    29821 tgctctcgcg ctttaataac atcacggtga tgaccaacag cctgcatatc gtcaatgcgc
    29881 tatccgaact ggataacgaa caaactatcc tgatgccagg cggaacattt cgcaaaaaat
    29941 cggcctcatt tcacgggcaa ctggcagaga atgccttcga gcatttcacc ttcgataaat
    30001 tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccacgttc aacgaggttt
    30061 ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg gcggactcat
    30121 caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc gataagctaa
    30181 ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct ggaagagaaa gggatcgatg
    30241 tgatcataac cggagagagc aatgagtgaa gcactactga acacgggacg tcagacgtta
    30301 atgctggagt tgcaggaagc aagccgttta ccggaacgtc tgggcgatga ttttgttcgc
    30361 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
    30421 ggccacattg gtaagaaaat cgccgcaaca cttgccagta ccggcacacc ggcttttttt
    30481 gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg cgatgtgatg
    30541 ctgtttatct cttactccgg tggcgcgaag gaactggatc tgattattcc gcgtctggaa
    30601 gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct gggcctggcg
    30661 gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg
    30721 acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc ggtcatgcag
    30781 gcgcgcggtt ttaatgaaga agattttgcc cgctcccacc cagctggggc actgggcgct
    30841 cgcttgctga ataaagtgca tcatctgatg cgtcgtgatg acgctatccc acaggtagcg
    30901 ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc gcaccggtct ggggctggtg
    30961 gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta ccgacggcga tttacgtcgc
    31021 tggctggtag gcggcggcgc actcaccacg ccagttaatg aagcgatgac gacaggcggc
    31081 accacattac aggcacaaag tcgcgccatc gacgccaaag aagttctgat gaaacgcaaa
    31141 atcactgccg caccagtggt agatgaaaac ggcaaactca ccggcgcaat taacctgcag
    31201 gatttctatc aggccgggat tatttaatcc ttcaatccca gacgtttcgc cagccgatgc
    31261 aggttggcga cgtcagtttc cagcattcgc gcacaggctg cccagttgtg atgattttgt
    31321 gccagtgcct ggcgaatagt ttcacgctgg aacgcttctg tcgcttcacg caggttttgc
    31381 ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa
    31441 tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg ctctcgccag cactaccgcc
    31501 cgatgaatag catgttccag ttcgcgcacg tttcccggaa aactgtagtg ttgcagtaaa
    31561 tttcgcgccc cggcacttaa taccacgcgg gagagcccct gccgcaaacg acactgctcg
    31621 cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcaccgaa
    31681 agtggaaaca cgctcaggcg atgaaacaaa tcggcgcgga atcgccctgc cagcacctct
    31741 tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat cgacccgcaa actacggtca
    31801 tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc
    31861 aacgacaact cgccgatctc atcgagaaac agagtaccgt tatccgccat ttcaaacttc
    31921 ccgctgcggt tactgatagc gccagtaaac gctcctttca catgcccgaa caactcactt
    31981 tccgccacac tttccggcag tgcggcacag ttgagataga ccagcggatt caccgcccgt
    32041 ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgctg
    32101 atcagaacgt tgagatcgga cgccgccaca atctcaatct cttttttcag ttgcgtcatg
    32161 ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt caaacggcgt ggcatcgcct
    32221 ggcagcatat tctggctttc cagttgttca atcagcaacg cattgcttaa cgctcccgcc
    32281 gccagcgcgg caatcagccg taactcttcg tcgctgaaaa catcgaactg atcgggctgc
    32341 atcccgtcga gcgtcagtgc gccgatcagg ttttgtccga caaacaatgg cagaccaacg
    32401 caggcgtgaa ccttcagact ctcctgccca ggaatcaaac cgtcataggg atcgggcaat
    32461 tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg caatcgcttc cagccgtgga
    32521 tgcccttcca gcgcaaagcg tctaccgagt acatcctttg ccagaccgtc gatggcaagc
    32581 ggaataaact gccgcgaatc gtaacgtagc aacgcagacg catcgcactc cagcacctga
    32641 cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg
    32701 atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga
    32761 ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact cattaatgag
    32821 cataatttta tttatagagt aaaaacaatc agataagaaa ctggcacgca atctgcaatt
    32881 agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga
    32941 aaaataacat tcattgggtg ggtcaacgtg actgggaagt gcgtgatttt cacggcacgg
    33001 aatataaaac gctgcgcggc agcagctaca acagctacct catccgcgaa gaaaaaaacg
    33061 tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg
    33121 aaatcgatct ggcggatatc gattacatcg tgattaacca tgcagaagag gaccacgccg
    33181 gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc gatctactgt actgccaacg
    33241 ctatcgactc gataaatggt catcaccatc atccggagtg gaattttaat gtggtgaaaa
    33301 ctggcgacac gctggatatc ggcaacggca aacagctcat ttttgtcgaa acaccaatgc
    33361 tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtgctg ttcagtaacg
    33421 atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagacgg
    33481 agcttttcga gcagtgccag cgttactacg ccaatatcct gacgccgttc agccgcctgg
    33541 taacgccgaa aattaccgag atcctgggct ttaacttacc agtcgatatg atagcgacct
    33601 cccacggcgt ggtctggcgc gataacccga cgcaaattgt cgagctgtac ctgaagtggg
    33661 ccgctgatta tcaggaagac agaatcacca ttttctacga caccatgtcg aataacaccc
    33721 gcatgatggc tgacgctatc gcccagggaa ttgcggaaac cgacccacgc gtggcggtga
    33781 aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa
    33841 aaggcgtgct ggtcggcact tcgacgatga ataacgtgat gatgccgaaa atcgccgggc
    33901 tggtggagga gatgaccgga ttacgcttcc gtaacaaacg cgccagtgct ttcggatctc
    33961 acggctggag cggcggtgcg atggatcgcc tttccacgcg cctgcaggat gcgggtttcg
    34021 aaatgtcgct cagcctgaaa gccaaatggc gaccagacca ggacgctctg gagttatgcc
    34081 gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg cagagcacgg
    34141 tgaatacggt agttaaagaa gaaacctctg ccacgacgac ggctgacctc ggcccacgaa
    34201 tgcagtgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca atgcaggacg
    34261 ttgcgccagg aacgccgtgg agtgaagtcc cggataactt cctctgcccg gaatgctccc
    34321 tcggcaaaga cgtctttgac gaactagcat cggaggcaaa atgagcaacg gcattgtgat
    34381 catcggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac aggacgccac
    34441 tattccatta accctgattg ccgccgacag catagatgag tacaacaaac ccgacctcag
    34501 ccatgttatc agtcaggggc aacgtgccga cgaccttacc cgccagacgg cgggtgaatt
    34561 tgccgggcag tttaatctgc gcctgtttcc gcacacctgg gtgacggata tcgatgccga
    34621 agcccatgtg gtgaaaagcc agaataatca gtggcaatac gacaagttag tgctggcaac
    34681 cggtgccagc gcctttgtcc cgcctgtgcc tgggcgtgag ttaatgctga cgttaaatag
    34741 tcagcaagag tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg tgttgattgt
    34801 cggcggtggt ttgattggta gcgaactggc gatggatttt tgtcgtgcag gcaaagcggt
    34861 cacgctaatc gacaacgctg ccagtattct ggcgtcatta atgccaccag aagtaagcag
    34921 ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttgaaat ctcagttgca
    34981 ggggctggaa aaaacggatt ctggcattct ggcaacgctg gaccaccagc gtagtatcga
    35041 agtggatgcg gtaattgccg ccaccggact gcgcccggaa acagccctgg cacgacgcgc
    35101 cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca gtaatgccga
    35161 tatttacgcg ctgggcgatt gcgcggaaat taacggtcag gtattgccgt tcctgcagcc
    35221 gattcaactt agcgggatgg tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa
    35281 actaccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc tggcaggcga
    35341 aacccagcgt caggatttac gctggcagat taataccgaa cgccagggaa tggtggcgcg
    35401 cggcgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga
    35461 ggcatttgta ttgttgaaaa cgttgccggt gtaggtgggc tactgtgcct aaaatgtcgg
    35521 atgcgacgct ggcgcgtctt atccgaccta cggggacgca tgtgtaggcc ggataaggca
    35581 tttacgccgc atccggcaag ggtgtccaaa tgcaacacgc tttatccgtt ctggacttca
    35641 cccgctaacc aacgcgccgc agcaataacc ccctgcccca gagacaaacc gccatcaccc
    35701 gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga gataatgcgc cagacgtgca
    35761 cgcagcaaac ggttatgaat aaccccgccg ctaaatacca gcgtagtgat accacgcatc
    35821 gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca gcgcatcatg aaacgcccac
    35881 gcgcgttgat taaccagtgc ctgccagttc agccactgct gccagaaagt tgcgagatcc
    35941 agttgattgt ccacccgcgg catcgtcacc ggatgcgtca ctccgtcgca tgaggctgcg
    36001 agcgcctcca gagcacaagc cgcttcacct tcataactta acgtggctgg cgcacagccc
    36061 agtgccgccg ccactgcatc gaacaaccgc ccacacgatg atgccagcgg cgcgttaatt
    36121 ccacgctcaa tggcccgcgc cagtacgctc cagttttgct gttgcacact tgccgtttca
    36181 gggtaattct gccactccgg cacaaagcgc aggcactgcg ccagcaggtt tcgccacggc
    36241 tgcttcgccg ccaaatcgcc acccggaagc gccactgcag gcaagccgcc caggtgctca
    36301 cattcgcgat agttcacccg caggcactcg ccgccccaca aagcgccatt ctcccccata
    36361 ccgataccgt cgagcgtcaa agcaatgaca tcaccgccat ccagcggcca ctgatgctct
    36421 gccagacacg ccgctgcatg ggcatgatga tgcagtaccg tttgcgtcgg cagattcatt
    36481 tcgctcgccc actggctgga gacatagccc ggatgcacat catgcacaat gtattgcgga
    36541 gtgaaatcgt agatgttttg catcaggcgt aacgcttcgc gccactgcat ctggatgcca
    36601 tcgtcactta aatcgcccag atgctgactc aacaccgctt gttcaccgcg caccaggcag
    36661 aaggtatttt tcagatccgc gccaagacac agcacaggcg gaacattttt aaagcccgga
    36721 ggcaaaacca gcgcatccgg cacatacccc cgcgaacggc gcagcatttc gccgctttcg
    36781 cgcaccactg aatcatccat ccgctgcacg atgtcgcggt tatgtagcaa gaatccgtcg
    36841 gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga tagctggtgg tttaccgctc
    36901 aggttgccgg aggtcatcac cagcgggcat tgcagttcct gtaacagcaa atgctggagc
    36961 gggttcgcag gcaacatcac cccgacttcg ttaaggccag gggcgatatc atcacaaagc
    37021 tcaggtacgt attttttatc caccagcaca atcggcgcag cgggcgtggt aagcaactgg
    37081 cgcgcagcgt ctggtaaact ttctgccact ggcaacatga ctgccagcgg tttcgccggg
    37141 cgatgtttgc gctcccgcag tgtcgccacc gcgttactgt tacgtgcatc gcaggcaaga
    37201 tgaaatccgc caatcccttt gatggcgaca atgttgccca tttttaactg tgcgatagct
    37261 gcctgtaatg ccgcctcttg ttctgcatgt tcaccatgac ttacccattc aagatgcggg
    37321 ccacactccg ggcaggccac cggctgggcg tggaagcgac gatcgagcgg gtcgcggtac
    37381 tctttgtcac aggccggaca tagcggaaac gccgccatca cggtaaacgg gcggtcgtaa
    37441 ggcatcgcgc gaataatggt gaaacgcggg ccgcagtggg tacagttgat aaacggataa
    37501 cgataacgcc gctcgcctgg ggtattcatt tcggcaaggc aagcagggca agtggcggca
    37561 tccgggacaa tttgcgtatt catggtgccg ccggtgctct ggcggatggt gaactcggtg
    37621 ggcagttgtg accagataaa cggctcacgc tcgacgctat caatacgcgc cagtggcgga
    37681 cagtgctgat gcaattgaac aagaaacgtt tccgggtctt ccagcagccg gacttctacg
    37741 ccatcgccgt cattacatac atcgccgtga agatttaatt gctgtgccag ctgccagaca
    37801 aacggacgaa aaccgacgcc ctgcactttg ccacgaatac gcagttggac accgcaagat
    37861 gtgttttttg ccattgagtt attcccgcca tcatgaattg cgtaacccgc cctgccggac
    37921 acgacagcgt cgtatccggc agtctcaggt cggcgatacc gcccgctccg tattctacga
    37981 atatttccgg gaattccttt gatgccagaa cagttctgta agatttttag aacatcagcg
    38041 ccgtacggcg gcgtttttct gcgctcagtt gttcaagttt attacgatcg acacaaatca
    38101 gcgcatgggt cgggcaagcc gccatacacg ccgggccgtc ttcacgatgg ttgcacaggt
    38161 cgcatttatt ggcttcggct ttgtcagccc gtacattcag acccgcgccg ctgttgcgaa
    38221 tcacaggacg taccaccact tccatcgcgc catacgggca agccaccacg caggttttgc
    38281 aaccaatgca acgttcctgc atcacaggaa caaacccttt atcacggctg atagcaccat
    38341 tcgggcagac gttagcgcac ggtgcatctt cacactgacg gcaaactgtc gccgtggaaa
    38401 tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt ttccggggtc agcgatgcac
    38461 agtcctgatt ttcctgatga gaaaccacgc acgccacttc acaggtacgg caaccaatac
    38521 atttactcgc gtcagcaatg atgaaacggt tcatcaaatt ctccagcaat gacagttaat
    38581 gcgccgatac actcacaaat catgccagtt tttaatttgc tgttatttaa ggaaattaat
    38641 ttctgtaatg caggaaaaaa gatgtcatcg acactagtga cgatgacatg tgatgacaat
    38701 gtttatcgcg aaagggcaat gagtgagtcg cggcggatca gttttccgct aaaggttttc
    38761 ggcggtgaga aatccccgcc atcgagcata aaaatcagcc gtccaataat ttcctgaatc
    38821 atctcagtca ccggaatttt tacgctggag agcgccggaa cgatataggg ggcaattgcg
    38881 atatcatcga atccgataac tgacacctgc tctggcaccg ctacgccgcg ctcgtgcaac
    38941 gctttgatcg cacctatcgc catatcgtcg ttactggcaa ctaacgcgct aaatttagcc
    39001 cctcgttcga gcaacgtttc taccccttcg gccccgctgg cgggcgtcca tttaccgtta
    39061 gcgataagtt tttcattgag cgcaatacca tgctgcgaca gcgcgtcttt atacccggca
    39121 agacgttcaa tgctggtggg ggaatccatc gagccggtaa ggaaagcaat ctcctgatgc
    39181 ccggcgttta tcaactctgc cacggcgtta aagctggtct gtttatgatc gcaccagacg
    39241 ctatggctgc tattattgcg caggcggcga ttaagcacca ttatcggctg actgtgcgcg
    39301 tcaatgatgt catcaatctc atccacgctt aaaaaacgcg ggtaaatcat aatggcgtcg
    39361 cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt cttcggcgct gtgtttacca
    39421 tctgccaata gcaactgtcg ccctttctct tccgccattc gcgcggcatg aaacagtaat
    39481 tcactaaaat aaatgccgtg ataaagcgtg ttggtcacta ccagccccag cgtctgagta
    39541 ctcttcgccg acagattgcg ggccagcaaa tttgggcggt aactgctctc ttctaccgcc
    39601 tgaaacacgc gatctttagt ctcctggctg acgtagccat tacctgaaag cacgcgggaa
    39661 acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca gcatcgtcgt catcattttc
    39721 atccctttac acgcaatcaa cgcagtgtac tgcaccgttt gccgattgtc cttgcgcaat
    39781 cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt
    39841 aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag
    39901 ttctttgtac ggcaccactt tgctcttctt cttacgcaca tcgactacgg tcatgcggtc
    39961 ggtacaggag tagcaagggt ccaggctacc gataatcagc ggcgcatagg aaacggtatt
    40021 gccacgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca
    40081 gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc
    40141 ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg
    40201 accgcccggc aggttatcca gaccgtagtc gatcatattc agcgcggtat agacctcgtt
    40261 gattcgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg
    40321 cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga gaccgctggc
    40381 gcgaaccatc gggccaacgt tactgaaatc gcgggcgatt tccgggtcca gacgaccaat
    40441 gccgacggtg cgttgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac
    40501 ttcacgacgc atttgttgcg ccagctggcg ggtctggatc atgtcatctt tcagcagatc
    40561 gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc
    40621 tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc
    40681 ggtaaagtga caggcgaggc cgaggttgag cagatgcggg tgcaggcgtt ccacctccag
    40741 cagaatggcg cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac
    40801 cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag
    40861 gaaggtcact tcgttataac ccatgcgggt ttccgccagt ttttccatac cgcgatggac
    40921 gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg
    40981 acccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc
    41041 gcccagttcg ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg
    41101 ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagcac
    41161 cagacgacgt tcatccggca gaccaaccgg aatcaaaccg tacatatcgc gcacttcgcg
    41221 ctcgccccac accgccgccg gaacgcgcgg cgtcacggac ggatactccg gtttgttggc
    41281 gtcaacttcg acgcgaaccg ttacccaaca cttggtgccc ttctccatcg acagcacgta
    41341 ataaacggcg taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca ccgacagcca
    41401 gccaccctgc ttgtagtaga gaaactccac cacttccggc agatagttca cctttacggt
    41461 gacagtcagc tgatctttgg tctgccaggc gtggtccagc acgacgccta gaaatgcctc
    41521 attcagcgcg gcgagataat gttgacctaa tttttcttca gacatgctca aactctcttt
    41581 aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc agcccaggta
    41641 atgcgcggag taatatcaag acgcagacgg gccatgctgt tttcgaacag cgcgataacc
    41701 aggacgccga ccaccagttt gacgatggca atcaccagcg ccagcagcag tccaccgacg
    41761 gtgaaggttt ccatttgtcc ccacggaata aacaccccga cgaacatctg caacaccacc
    41821 agctgtttca ggctgatacc ccatttcatg acgccaaagc cgctaccgct gtattcggag
    41881 agcggacctt cctgcaactc ctgctcggct tccgccaggt cgaacggcag tttgcccatt
    41941 tcgataaaag tggcgaacgc acaggcgcag agcgccagca ccagcgggat gctttggctc
    42001 agcggccagt gataaacggt gtcggtaatg ttgctgatgt tggtggaacc ggcaacctgt
    42061 gcggcaaccc acagaccaag cagcaacatc ggttcgacca gcacgccaag catcgcttca
    42121 cggctcgcac cgatagcggt aaacgggcta ccggtatcca gaccggaaat ggcaaagaag
    42181 aaacgcgcga tggcgaagag atacagtaag gtgatcaaat cgcccagttg cggcagcgga
    42241 gaaccgacgg tcaccaccgg cagcgcagta gcgatagtca gcatgacgcc caccatcaca
    42301 tacggcgtca ggcggaacat ccagccagag gcatccgggc caacgctctg gcggcccagc
    42361 agtttgataa tgtcgcgata ctcctgcaac acgcccggcc cgcgacggtt atgcagacgg
    42421 gcgcgcgcca cgcgggtaat accggagagc agcggcgcaa cggcaaataa caccagcgcc
    42481 tgaattaacg gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca
    42541 gtaccgccag ttcaaccagc gccatccggc ggaacagcaa cgcactacct tcgcactgcc
    42601 agcccggcac tagcgacacc ggattcagcc atttgcgcag tttcagcact ggcgcaaacg
    42661 cctgtttaac cggcatggca aaaccgtgag cggtaatcac cattgatttt tcatggtcgt
    42721 agccgcacac ccaggccgca ccgcgggaac gtgacggcag gcggtcgcct ttgcaaatcg
    42781 ccataatgat gaatggcagc agcgggcagg caatcagcag caatgtgatc attggttgag
    42841 aaacggtggt gttagcaggc tccagaggca gaggtacagc agcagagagc atcggcagca
    42901 gccacggcgc agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttacgc
    42961 tcatcaggag cggcgcacag gtggcgtttt cggcttcttt ggtacgcggc gcgccgagga
    43021 acgtgacgcc atagactttc gccatacaca tcaccgccag cgcaccggta attgccagac
    43081 ccacagcgag cagcggcccc agcagacggg caacaaacgc gccgctattg ctcagtttga
    43141 agaaggattg atagataacc cattccccgg caaacccgtt cagtggcggc agcgcagcca
    43201 ttgccatcaa cccgactaac atggcgatgg agataaccgg cattttcttg ccaataccac
    43261 cgagtttttc gatatcgcga tgaccggtac ggaaccagac gctccccgcc ccgaggaaca
    43321 gtacgctttt gaacaggcta tggttaagca gatggtacag accaccgacc aggccaagag
    43381 caatcagcgc cggttgttcg agcgcg
//
LOCUS       DAZOZX010000021        42013 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZOZX010000021 DAZOZX010000000
VERSION     DAZOZX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.021"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..259
                     /locus_tag="ACHUPT_001099"
     CDS             <1..259
                     /locus_tag="ACHUPT_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818757.1"
                     /translation="LGMHNLILLENICMQKNAECMAHITFTLNKLTTQPAITKIDSVG
                     NEQLYQIGKIYHKLEIKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(319..810)
                     /gene="yajQ"
                     /locus_tag="ACHUPT_001100"
     CDS             complement(319..810)
                     /gene="yajQ"
                     /locus_tag="ACHUPT_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0818758.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            978..1889
                     /gene="panE"
                     /locus_tag="ACHUPT_001101"
     CDS             978..1889
                     /gene="panE"
                     /locus_tag="ACHUPT_001101"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0818759.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            1852..2442
                     /gene="yajL"
                     /locus_tag="ACHUPT_001102"
     CDS             1852..2442
                     /gene="yajL"
                     /locus_tag="ACHUPT_001102"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR0818760.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2496..3944)
                     /gene="thiI"
                     /locus_tag="ACHUPT_001103"
     CDS             complement(2496..3944)
                     /gene="thiI"
                     /locus_tag="ACHUPT_001103"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0818761.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4150..4392
                     /gene="xseB"
                     /locus_tag="ACHUPT_001104"
     CDS             4150..4392
                     /gene="xseB"
                     /locus_tag="ACHUPT_001104"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0818762.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4392..5291
                     /gene="ispA"
                     /locus_tag="ACHUPT_001105"
     CDS             4392..5291
                     /gene="ispA"
                     /locus_tag="ACHUPT_001105"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0818763.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5316..7178
                     /gene="dxs"
                     /locus_tag="ACHUPT_001106"
     CDS             5316..7178
                     /gene="dxs"
                     /locus_tag="ACHUPT_001106"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0818764.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7233..8150
                     /gene="yajO"
                     /locus_tag="ACHUPT_001107"
     CDS             7233..8150
                     /gene="yajO"
                     /locus_tag="ACHUPT_001107"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0818765.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8261..8779)
                     /gene="pgpA"
                     /locus_tag="ACHUPT_001108"
     CDS             complement(8261..8779)
                     /gene="pgpA"
                     /locus_tag="ACHUPT_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0818766.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(8757..9734)
                     /gene="thiL"
                     /locus_tag="ACHUPT_001109"
     CDS             complement(8757..9734)
                     /gene="thiL"
                     /locus_tag="ACHUPT_001109"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0818767.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(9812..10231)
                     /gene="nusB"
                     /locus_tag="ACHUPT_001110"
     CDS             complement(9812..10231)
                     /gene="nusB"
                     /locus_tag="ACHUPT_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0818768.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10251..10721)
                     /gene="ribE"
                     /locus_tag="ACHUPT_001111"
     CDS             complement(10251..10721)
                     /gene="ribE"
                     /locus_tag="ACHUPT_001111"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0818769.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(10810..11913)
                     /gene="ribD"
                     /locus_tag="ACHUPT_001112"
     CDS             complement(10810..11913)
                     /gene="ribD"
                     /locus_tag="ACHUPT_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0818770.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(11917..12366)
                     /gene="nrdR"
                     /locus_tag="ACHUPT_001113"
     CDS             complement(11917..12366)
                     /gene="nrdR"
                     /locus_tag="ACHUPT_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0818771.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12517..13056
                     /locus_tag="ACHUPT_001114"
     CDS             12517..13056
                     /locus_tag="ACHUPT_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0818772.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13354..14238
                     /gene="tsx"
                     /locus_tag="ACHUPT_001115"
     CDS             13354..14238
                     /gene="tsx"
                     /locus_tag="ACHUPT_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0818773.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14415..14762)
                     /gene="yajD"
                     /locus_tag="ACHUPT_001116"
     CDS             complement(14415..14762)
                     /gene="yajD"
                     /locus_tag="ACHUPT_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0818774.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(14891..15862)
                     /gene="secF"
                     /locus_tag="ACHUPT_001117"
     CDS             complement(14891..15862)
                     /gene="secF"
                     /locus_tag="ACHUPT_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0818775.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(15873..17720)
                     /gene="secD"
                     /locus_tag="ACHUPT_001118"
     CDS             complement(15873..17720)
                     /gene="secD"
                     /locus_tag="ACHUPT_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0818776.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(17748..18080)
                     /gene="yajC"
                     /locus_tag="ACHUPT_001119"
     CDS             complement(17748..18080)
                     /gene="yajC"
                     /locus_tag="ACHUPT_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0818777.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18103..19230)
                     /gene="tgt"
                     /locus_tag="ACHUPT_001120"
     CDS             complement(18103..19230)
                     /gene="tgt"
                     /locus_tag="ACHUPT_001120"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0818778.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLLEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19285..20355)
                     /gene="queA"
                     /locus_tag="ACHUPT_001121"
     CDS             complement(19285..20355)
                     /gene="queA"
                     /locus_tag="ACHUPT_001121"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0818779.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20448..21021
                     /gene="acpH"
                     /locus_tag="ACHUPT_001122"
                     /pseudo
     CDS             20448..21021
                     /gene="acpH"
                     /locus_tag="ACHUPT_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21026..22843)
                     /gene="malZ"
                     /locus_tag="ACHUPT_001123"
     CDS             complement(21026..22843)
                     /gene="malZ"
                     /locus_tag="ACHUPT_001123"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0818780.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(22999..24372)
                     /gene="proY"
                     /locus_tag="ACHUPT_001124"
     CDS             complement(22999..24372)
                     /gene="proY"
                     /locus_tag="ACHUPT_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0818781.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24448..25767)
                     /gene="brnQ"
                     /locus_tag="ACHUPT_001125"
     CDS             complement(24448..25767)
                     /gene="brnQ"
                     /locus_tag="ACHUPT_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0818782.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26174..27469)
                     /gene="phoR"
                     /locus_tag="ACHUPT_001126"
     CDS             complement(26174..27469)
                     /gene="phoR"
                     /locus_tag="ACHUPT_001126"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0818783.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27527..28216)
                     /gene="phoB"
                     /locus_tag="ACHUPT_001127"
     CDS             complement(27527..28216)
                     /gene="phoB"
                     /locus_tag="ACHUPT_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0818784.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28406..29608
                     /gene="sbcD"
                     /locus_tag="ACHUPT_001128"
     CDS             28406..29608
                     /gene="sbcD"
                     /locus_tag="ACHUPT_001128"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0818785.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            29605..32748
                     /gene="sbcC"
                     /locus_tag="ACHUPT_001129"
     CDS             29605..32748
                     /gene="sbcC"
                     /locus_tag="ACHUPT_001129"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR0818786.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            32874..34055
                     /gene="araJ"
                     /locus_tag="ACHUPT_001130"
     CDS             32874..34055
                     /gene="araJ"
                     /locus_tag="ACHUPT_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0818787.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(34198..35106)
                     /gene="mak"
                     /locus_tag="ACHUPT_001131"
     CDS             complement(34198..35106)
                     /gene="mak"
                     /locus_tag="ACHUPT_001131"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0818788.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            35231..36142
                     /gene="rdgC"
                     /locus_tag="ACHUPT_001132"
     CDS             35231..36142
                     /gene="rdgC"
                     /locus_tag="ACHUPT_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0818789.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36220..36675)
                     /locus_tag="ACHUPT_001133"
     CDS             complement(36220..36675)
                     /locus_tag="ACHUPT_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818790.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(36672..37007)
                     /locus_tag="ACHUPT_001134"
                     /pseudo
     CDS             complement(36672..37007)
                     /locus_tag="ACHUPT_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37162..37446)
                     /gene="ppnP"
                     /locus_tag="ACHUPT_001135"
     CDS             complement(37162..37446)
                     /gene="ppnP"
                     /locus_tag="ACHUPT_001135"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0818791.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(37518..38195)
                     /locus_tag="ACHUPT_001136"
     CDS             complement(37518..38195)
                     /locus_tag="ACHUPT_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0818792.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(38453..38644)
                     /gene="yaiA"
                     /locus_tag="ACHUPT_001137"
     CDS             complement(38453..38644)
                     /gene="yaiA"
                     /locus_tag="ACHUPT_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0818793.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(38694..39218)
                     /gene="aroL"
                     /locus_tag="ACHUPT_001138"
     CDS             complement(38694..39218)
                     /gene="aroL"
                     /locus_tag="ACHUPT_001138"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0818794.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(39401..39859)
                     /locus_tag="ACHUPT_001139"
     CDS             complement(39401..39859)
                     /locus_tag="ACHUPT_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0818795.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            39979..40788
                     /gene="proC"
                     /locus_tag="ACHUPT_001140"
     CDS             39979..40788
                     /gene="proC"
                     /locus_tag="ACHUPT_001140"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0818796.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(40805..41920)
                     /gene="adrA"
                     /locus_tag="ACHUPT_001141"
                     /gene_synonym="dgcC"
     CDS             complement(40805..41920)
                     /gene="adrA"
                     /locus_tag="ACHUPT_001141"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0818797.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10527 a        11275 c        10515 g         9696 t
ORIGIN      
        1 gttgggtatg cataatctga tattgttgga gaatatttgt atgcaaaaaa atgcagaatg
       61 catggcacac attaccttta cgcttaataa attaacaaca caaccagcaa taacaaaaat
      121 agatagcgtg ggtaatgagc aactttatca gattggcaaa atctaccata agttggaaat
      181 taaacctgag ccagagcaac aggttttaat tcagtcgctt tatgaccggg catcaggaag
      241 cgcctctgca actcaataaa tgcatggtca aaagggccac cacggtggcc ctgacaatca
      301 gtagctatat ccagaaagtt aatcgcggaa gtttttgaac tggaacggct gaccgagatc
      361 gccaccgcgc accatcgcca tgacagcctg caaatcatca cgagatttgc cagtcacgcg
      421 gatctcatcg ccctgaattt gcgcctgcac tttcagtttg ctgtctttga tcatcttgac
      481 gatttttttc tgagtggcgc tctcaatgcc ctgtttcagt ttcgcttcca caaaccaggt
      541 tttaccgcta tgaacgatat tttccggtac atccagtgaa ctgccttcaa tgccgcgctt
      601 cagcagcttg gcacgcagaa tatccagcaa ctgattgacc tggaagtcgg actcgctcaa
      661 cactttgatg gttttgctgg cgtcgttcag ctcaaatgag gcttcaacgt tacggaagtc
      721 aaaacgggac tccacttcgc gactcgcgtt atcgaccgcg ttacgtgctt cctgaagatc
      781 aacttcagag acaatatcga aagatggcat cttttcctct cccttcattt ttgatgcgaa
      841 gcataatacc tgcaaagtta aataaccaac agtcctgcac caggacgtat cccttaattg
      901 tgcatagtcg caacagcatg cacaattaag ggatagcctt attgaacacc cggagtggtt
      961 gcgggtgagg aggaacaatg aaaattaccg tattgggatg cggtgcctta gggcaattat
     1021 ggcttacagc actttgtaaa cagggtcatg aagttcaggg ctggctgcgt gtaccgcaac
     1081 cttattgtag tgtgaatctg gttgagacag atggttcgat atttaacgaa tcgctgaccg
     1141 ccaacgatcc cgattttctc gccaccagcg atctgctcct ggtgacgctg aaagcatggc
     1201 aggtttccga tgccgtcaaa agcctcgcat ccacactgcc tgtaactacg ccaatactgt
     1261 taattcacaa cggcatgggc accatcgaag aattgcaaaa cattcagcag ccattactga
     1321 tgggcaccac cacccatgcc gcccgccgcg acggcaatgt cattattcat gtggcaaacg
     1381 gaatcacgca tatcggtccg gcacggcaac aggatggcga ttacagttat ctggcggata
     1441 ttttgcaaac cgtgttgcct gacgttgcct ggcataacaa tattcgcgcc gagctgtggc
     1501 gcaagctggc cgtcaactgc gtgattaatc cactgactgc catctggaat tgcccgaacg
     1561 gtgaattacg tcatcatccg caagaaatta tgcaaatatg cgaagaagtc gcggcagtga
     1621 tcgaacgcga agggcatcat acttcagcgg aagatttgcg tgattacgtg atgcaggtga
     1681 ttgatgccac agcagaaaat atctcgtcga tgttgcagga tatccgcgcg ctgcgccaca
     1741 ccgaaatcga ctatatcaat ggttttctct tacgccgcgc ccgcgcgcat gggattgccg
     1801 taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa ggagagtgaa tatgagcgca
     1861 tcggcactgg tttgcctcgc ccctggtagt gaagagactg aagccgtcac cacgatcaac
     1921 ctcttggttc gcggcggtat caaagtcacc actgccagcg tcgacagcga tggcaacctg
     1981 gcaattacct gctcgcgcgg cgtgaagctg ctggcggatg cgccgctggt cgaagtggct
     2041 gatggcgaat atgacgtgat cgtgctgcct ggtggcatta aaggcacgga gtgttttcgc
     2101 gatagcactc tgctggttga aaccgttaaa cagttccacc gttccgggcg tatcgtcgcg
     2161 gctatttgcg ccgcgccagc caccgtgctg gtgccgcacg atatctttcc gattggcaat
     2221 atgaccggct tcccgacgct gaaagacaaa attcccgctg aacaatggca ggacaagcgc
     2281 gtcgtctggg atgcacgggt aaaattgctg accagccagg ggccgggtac agctatcgac
     2341 tttggtctga aaattatcga cctgctggtt gggcgcgaga aagcccatga agtggcatca
     2401 caactggtga tggcggcagg gatttataat tattacgagt agtgtcggat gcggcaaacg
     2461 tcgcatctga ccagatgcga cgtaaaaact acaaattacg ggcgatatac cttcacattg
     2521 ttaaagcctt gctcgcgcag atagagcgcc tgcagacggc tcatcacccc gcgctcacac
     2581 cacagcagcc aggttttgtt ctggtcgaga tcgccaaatt tggtgctcag tttatagaac
     2641 ggcagagaaa ccacatcaat cccttcgact ttcagtggct tatcttcctg ttcatcgaca
     2701 gaacggatat cgagaatcac gtcgttcggg ccgaagccgt tgacggtttc cacttccacc
     2761 acttcctgct cggtctgctg ggcgatttcg cggatatcaa cgttattcgc ttcctcaacc
     2821 actttatcga gaatactgaa gtcgaacttc tcttcttccg cttcaatctt cgatttaacc
     2881 gctttcaccg tcgggctttt ggagatcacg ccacaatatt ccggcatcgt gcgagcaaag
     2941 tcttcggtgc caatctggcg agccaggttg atgatgtgct ctttgtcgta agaaatcagc
     3001 ggacgcagga tcagcgtgtc agagacgtta tcaatcaggc gcaggttggt cagcgtctga
     3061 ctggacacct ggccgagcgc ttcgccggtg accagcgcct gtacgccgta acgttcagcc
     3121 actttagatg cggcacgcac catcatacgt ttgaggataa cgcccatctg accgtcgtcg
     3181 attttctcga gaatttcccc gacaaccggt tcgaaattaa tggcgacaaa acgcacgcgg
     3241 tgggagctgc caaagcggtt ccacagataa tgcgccacct gacgcacgcc aatttcatgc
     3301 gctgcgccgc cgaggttaaa gaagcagtaa tgcacgcggc agccgcgacg catcaacata
     3361 taactggaaa caccggagtc gaaaccaccg gaaatgagcg acagcacatc ttcctgggtg
     3421 ccaatcggga aaccaccaat accttcgtag cgacctttaa tcagcaggag gcgatcgtct
     3481 tccacttcca gatgaacagt tacatccggg ttagtcagct tcacgcgcgc ggattcaata
     3541 tgctgattta aaccgccgcc gacgtaacgt tccacatcaa tcgagctaaa atcatgtttg
     3601 ccacggcgct tcacgcgtac gcagaaggtt ttgccttcca gctgatcgcg atactgaacc
     3661 aacgctttct caaaaatatc gtgcatgtcg gtaaacggca cgtcttcgac ttcgagaata
     3721 tggtggatac ccggaatacg ggtcagggcg tcgcgaatag tcagacgctg gttttcatct
     3781 ttcgcgcgaa cttcgatgtt atcccagtgg cggacgacag cgagcgtctc atcatagtgc
     3841 tttaaaacgt tacgaatgtt cccggtaagg atttttataa agcgcaagcg cacagattgg
     3901 cttttgatgg tgatttccgg gaacaattta atgataaact tcatggcggc aatggttcgt
     3961 tggcaagcct taagcgactt gtatagggaa aaatacagca gcccacacct gcggctgcat
     4021 ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc acatcacctt accattgcgc
     4081 gttatttgct atttgccctg agtccgttac catgacgggg cgaaaaatat tgagagtcag
     4141 acattcatta tgccgaagaa aaatgaggcg cccgccagct ttgaaaaggc gctgagcgag
     4201 ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc cgctggaaga ggcgctgaac
     4261 gagttcgaac gcggcgtgca gctggcacgt caggggcagg ccaaattaca acaagccgaa
     4321 cagcgcgtac aaattctgct gtctgacaat gaagacgcct ctctaacccc ttttacaccg
     4381 gacaatgagt aatggacttt ccgcagcaac tcgaagcctg cgttaagcag gccaaccagg
     4441 cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc gaaaccatgc
     4501 agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat gccaccggtc
     4561 atatgtttgg cgttagcaca aacacgctgg acgcacccgc tgctgccgta gagtgtatcc
     4621 acgcttactc attaattcat gatgatttac cggcgatgga tgatgacgat ctgcgtcgcg
     4681 gtttgccgac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct ggcgacgctt
     4741 tacaaacgct ggcgttctcg attctaagcg atgccaatat gccggaagtg tcggatcgcg
     4801 acagaatttc gatgatttct gaactggcga gcgccagcgg tattgccgga atgtgcggtg
     4861 gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg cttgagcgta
     4921 ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt gcattaagcg
     4981 ccggagataa agggcgtcgt gctctgccag tactcgacaa gtacgcagag agcatcggcc
     5041 ttgccttcca ggttcaggat gacatcctgg atgtggtagg agatactgca acgttgggaa
     5101 aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt ctgggtcttg
     5161 agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcaggcg ctgaaacaac
     5221 tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac atcatccagc
     5281 gtaataaata aacaataagt attaataggc ccctgatgag ttttgatatt gccaaatacc
     5341 cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac
     5401 cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc
     5461 acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca
     5521 ccccatttga ccaattgatt tgggatgtgg gacatcaggc ttatccgcat aaaattttga
     5581 ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac ccgttcccgt
     5641 ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg
     5701 ccggaattgg tattgcggtt gctgccgaga aagaaggcaa aaatcgccgc accgtctgtg
     5761 tgattggcga cggcgcgatt accgctggca tggcgtttga agcgatgaat cacgcgggcg
     5821 atattcgtcc tgatatgctg gtggtcctca acgacaatga aatgtcgatt tccgaaaatg
     5881 ttggcgcgct taacaaccat ctggctcagc tgctttccgg taagctttac tcttcactgc
     5941 gcgaaggcgg gaaaaaagtt ttctctggcg tgccgcccat taaagagctg ctcaaacgta
     6001 ccgaagaaca tattaaaggc atggtggtgc ctggcacgtt gtttgaagag ctgggattta
     6061 actacatcgg cccggtggac ggtcacgatg tgctggggct tatcaccacg ctaaagaaca
     6121 tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg
     6181 aaccggcaga aaaagacccg atcactttcc acgccgtgcc taaatttgat ccctccagcg
     6241 gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt ggcgactggt
     6301 tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg atgcgtgaag
     6361 gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ttacttcgac gtggctattg
     6421 ccgagcaaca cgcagtgacc tttgccgccg gtctggcgat tggtggatac aaacccattg
     6481 tcgcgatcta ctccaccttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcaa
     6541 ttcaaaagct accggtcctg ttcgccattg accgcgcggg cattgttggt gctgacggtc
     6601 aaactcacca gggcgctttt gatctctctt acctgcgctg cataccagaa atggtcatta
     6661 tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg
     6721 atggcccgtc cgcggtgcgc tacccgcgcg gcaacgcggt tggcgtggaa ctgacgccgc
     6781 tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa ctggtgatcc
     6841 ttaactttgg tacgctgatg ccagaaacgg ccaaagtcgc cgaatcgctg aacgctacgc
     6901 tggtcgatat gcgttttgtg aaaccacttg atgaagcgtt aattctggaa atggccgcca
     6961 gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca ggtagcggag
     7021 tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt ggcctgccgg
     7081 acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg
     7141 ccggtatgga agccaaaatc aaggcctggt tggcataatc cctactccac tcctgctatg
     7201 cttaagaaat tattcatggc aggagtggca gcatgcaata caacccctta ggaaaaaccg
     7261 accttcgcgt ttcccgactt tgcctcggct gtatgacctt tggcgagcca gatcgcggta
     7321 atcacgcatg gacactgccg gaagagagca gccgtcccat aattaaacgc gcgctggaag
     7381 gtggcataaa tttctttgat accgccaata gctattccga tggtagcagc gaagagatcg
     7441 ttggtcgcgc actgcgggat ttcgcccgtc gtgaagacgt agtcgttgcc accaaagtgt
     7501 tccatcgcgt tggtgattta ccggaaggat tatcccgtgc acaaattttg cgctctatca
     7561 acgacagcct gcgacgtctc ggcatggatt atgtcgatat cctgcaaatt catcgctggg
     7621 attacaacac gccgatcgaa gagacgctgg aagccctgaa cgacgtggta aaagccggga
     7681 aagcgcgtta tatcggcgcg tcatccatgc acgcttcgca gtttgctcag gcgctggaac
     7741 tacaaaaaca gcactgctgg gcgcagtttg tcagtatgca ggatcactat aatctgattt
     7801 atcgcgaaga agagcgcgag atgctgcctc tgtgttatca ggagggcgtg gcggtgattc
     7861 cgtggagccc gctggcgcgg gggcgactga cgcgtccatg gggagaaact accgcacgac
     7921 tggtgtctga tgaggtgggg aaaaatctct ataaagaaag cgatgaaaat gacgcgcaga
     7981 tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc aacacgagca caagttgcgc
     8041 tggcctggtt gttaagtaaa ccgggcattg ccgcaccgat tatcggaact tcgcgggaag
     8101 aacagcttga tgagctgctg aacgcggtgg atatcacttt gaagccataa cagattgccg
     8161 agctggaaac gccgtataaa ccgcatgcgg tcgtaggatt taaataagag tccaggcctg
     8221 atgagacgtg acaagcgtca catcaggcat cggtgcacaa ctacgacaga atacccagcg
     8281 gccagtgatg accgataaag tacaggatgc ctgcggaaat caccccggcg acaatatcgt
     8341 cgatcatgat ccccatgccg ccatgtacat tgcgatcaaa ccagcggatc ggccacggct
     8401 tccacatatc cagaatacgg aaaatcacaa acccggcggc aacccactgc cagtcattgg
     8461 tcggcagcgc catgagcgtg atccacatac caataaattc gtcccagaca atgctgccgt
     8521 gatcgtgcac gcccatatct ttcgccgttt gatgacaaag atagacgccg atacagatcc
     8581 ccaacatcac caccagcgag tagagctgcc agggtaaaaa ggtcatcaga taccagaaag
     8641 ggatcgccgc cagcgatccc atcgagccag gaacgattgg gcttaatcca cttccgaatc
     8701 cgacagcaag cagatgccac ggattactca tcttcaggcg acttttcgcg acatctttat
     8761 ggcgtgacaa aatggtcata tcctttccag tcaaacgtga caggttcgcc gtcacgaata
     8821 aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac aggtaaacgg tacgccaagg
     8881 tgtccgagag caacatccag cgcgccacgg ttcagttccg gcaccgtgaa acacaactcg
     8941 taatcttcac cgccagagag cgcccagcgc agcgcctgtt ccggttcaac atggcgagaa
     9001 agcgcatcag aaaacggcag caatgccagg tcaatacgtg cgccgcagtc gctggctttc
     9061 acgatatgcc cgagatcgga aatcagaccg tcagagagat cgatagctga atttgccaga
     9121 tcgcgcagtg cctgcccctg taaaatacgc ggcgatggac ggagatgacg tttgatcaag
     9181 tagtcagcat ctttagcatc ggcaacctgc aaacggtttt gcaaaatcgc cagcccggcg
     9241 gcgctatcgc ccggtgtacc ggtcacatag atccagtcac ccggtttcgc cccagagcgc
     9301 gttaaggctc gtcccatcgg aacaaagccg tggataccca acgtcattga taatggccca
     9361 cgcgtggtat cgccgccaat gagttgcata tcgtaataat tgagaagatc aaacaaactg
     9421 tcgctgaagg actcaagcca cgcttcgtct acgtccggta aggttaatgc cagcgtcagc
     9481 caggccggat cggcccccat cgctgccaga tcgcttaggt tcaccgccag tgctttataa
     9541 gccagatcag caggatcgat atcagggagg aaatggttgc ccgccaccag cgtatcagtg
     9601 ctgatcgcca gggtctgttt ctcggggata ttgagaagag cgcaatcgtc gccgatgccc
     9661 agttcgacat caagacgaga acttcttaca cggtcaaaat aacgggcaat cagggagaac
     9721 tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag aaaaggccgg cgatcatgga
     9781 acagtcttcc gtgaatctac cggcctggat attacttttt gttagggcga atcacaggtg
     9841 ctgctttatc gagtacgccg ttgacgaact tatggctgtc ttctgcgccg aacgatttcg
     9901 ccagttcgat cgcttcgtta atggccactt tgtatggcac atcactacgt ttagacagtt
     9961 cgtacagcgc aatgcgcagt actgcttttt ctacctggcc cagttcttcc agcaggcggg
    10021 acaggtaggg cttcatcagt ccgtcgaggt atgcggtgtt agtcgccacc ccggccagca
    10081 gctcacggaa gtacaggacg tcaacgtctt ttacatcctg ttcagccagg aactggtatt
    10141 caacatcagc gatgtcgttc tgggacaact gccaggagta gagcgcctgg acggcacact
    10201 cacgagcgcg gcgacgagca gcaggtttca cggatttccc cttactaatt tcaggccttg
    10261 atggctttca atacattaat catttcaagc gcggtcagtg cagcttctgc acctttgttg
    10321 ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc tttcagtggt cagaacccca
    10381 aaagcaaccg gaatttcgct gtcctgggca acatgcgcca ggccgttgct tgcaccacca
    10441 gcgacatatt caaagtgggc agtgccacca cgaataaccg tacccagcgc aatcaccgcg
    10501 tcgtatttac cggttttagc cagtgcaccc gccgccagcg gcagctcata ggcaccaggc
    10561 acccaaacaa cggtaatgtt ttcatctttt acctgaccga tacgtttcag tgcgtcaatt
    10621 gcaccttcca gcaggctgtc attgataaag ttgttgaaac gcgcgatggt gatggcgacg
    10681 cgagcgtccg gggtagcaac gttagcttca ataatgttca ttttcttcct tcgggttcga
    10741 gtatggcccc gcaggggggc ggattttagc ataatatttc gtgcgctgct tccctttcga
    10801 gccgggagat catgcaccca ctaaatgcag gcaaacatcc gggcctacat gacgtatctc
    10861 tttgaattta aattgggggg cgtcggctaa tttctcaagc cctggcagcg agcataatcc
    10921 acgggcgtcg ctgcctaata gtttaggtgc gatatagaca atcagctcat cgactaaacc
    10981 cgcctgtagc aatacgccag cgagcgttgg ccccgcttcc acccagatgc tgttaatctg
    11041 ctgtttaccc agttgcatca tcagtacaac cagatccaga tgacctttat gctctggaat
    11101 cagcaaggta cgcaccgttt ccggccactc acgagaatct tcctgcgtac gcgcgaacca
    11161 ggtttcgccg ggctgctgca caatgcgatg ttccggcgtc acgcgatttt ggctatcaat
    11221 cacaatacgt actggctggc ggagattttg ttgcgggtag agcgcctgag tttgttcatc
    11281 cagttcagac caacgcactg ttaaggctgg atcatccgcc agcaccgtgg cgctgctggt
    11341 taaaatggca tgactttgtg cgcgctgtcg ttgtacgtcg cgccgcgcct gaggcgaagt
    11401 gatccactgg ctttcgccgc tcgccatcgc cgtgcggcca tcaagcgatg cgccaagttt
    11461 taactgaata taaggaaagc cggtgcgcat ccgcttgaga aagcctttat tcaattgctc
    11521 ggcttcactc atcatcagcc cgtggctgac gtcaatgcca gcctgttgca gacggtaaag
    11581 tccacgccca gcgacctgcg ggttaggatc ttgcatcgct gcgaccacgc gcgctacgcc
    11641 agcggcgatg agtgcgtcac agcacggtgg cgtacggcca tgatggctac agggttcgag
    11701 tgtcacatac gccgttgcgc ctttggcttt ttcacccgcc atacgcaacg cgtgtacttc
    11761 ggcatgtggt tcacccgctc gttggtggta accttcaccg acaatttcgc catctttgac
    11821 aatgacgcac ccgacattcg ggttggggtg ggtggtaaaa cgtcctcgtt gcgccagctt
    11881 tagcgcccgc gccatgtaat actcgtcctg cacggcttag tcctccaggc gcgcgatctc
    11941 ttcgccaaat tctttgatat cttcgaaact gcggtagaca gaggcaaaac ggatataggc
    12001 gactttatcg agctttttca attgctccat caccagattg ccaatcatct tgctcggtac
    12061 ttcgcgctca ccggtggcgc gcagctgcga tttaatatga ttgattgcca tttcaacgta
    12121 atcggaactc accggacgtt tttccagcgc ccgcagcatt ccgctacgca atttctcttc
    12181 attaaacggt tcacgcacgt cgttgctttt tacaacacgc ggcataacca gctccgccac
    12241 ttcaaaggtg gtgaaacgtt cattacacac cagacactgc cgacggcggc gtacggatga
    12301 accctcgccc acgagacgag agtcaattac cttagtgtcc acggcgaaac agaatgggca
    12361 atgcatacgg cgtcctgacc aggtggttaa cggaaatcta ttttaccctg aactgacgat
    12421 acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc tttcgctgct tttaccctct
    12481 accattaggc taattgcgac gagaaaggga cgacctatga atactaacgt ttttcgactg
    12541 ctcctgctgg gaagcctgtt cagccttagc gcctgtgtgc agcaaagtga agtgcgacag
    12601 atgaaacaca gcgtcagcac gctgaaccag gagatgacgc aactcaatca agaaacagtc
    12661 aaaatcactc agcaaaacag gctgaatgca aaatccagca gtggggttta ccttctgcct
    12721 ggagcgaaaa caccggcaag actggaaagc cagatcggta ctttacgtat gtcgctggtg
    12781 aatattacgc ctgatacaga tggcaccacc cttacactac gtattcaggg tgagtcgaat
    12841 gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac
    12901 aactttcagg aaatcaatgt gcagaatcaa ttgattaatg cccctgccag cgtcctcgcc
    12961 cccagcgatg ttgatattcc gttacaatta aaggggattt ctgtggatca gttaggcttt
    13021 gtacgtatcc acgacattca acctgttatg cagtaaacgt atttcgggac gattttgtgc
    13081 gtcccgcaac atcttccccg tcattttgtt actctgctta catcacctgg attgatagta
    13141 aaagtttgca acaagggcga aagtcagtac aatccccgcc cgaatgtgtg taaacgtgaa
    13201 cgcaatcgat tacgtaaatg atagaactgt gaaacgaaac atatttttgt gagcaatgat
    13261 ttttataata ggctcctctg tatacgaaat atttagaaac gcaatttgcg cctttttcac
    13321 tcccgcaagg gattttcaaa cagtggcata catatgaaaa aaacattact ggcagccggt
    13381 gcggtactgg cgctctcttc gtcttttact gtcaacgcag ctgaaaacga caaaccgcag
    13441 tatctttccg actggtggca ccagagcgtt aacgttgtcg gaagctatca cacccgtttc
    13501 ggaccgcaga tccgcaacga tacctacctt gagtacgaag cattcgctaa aaaagactgg
    13561 ttcgacttct atggttatgc ggatgcgccg gtattcttcg gcggtaactc cgatgctaaa
    13621 ggtatctgga accacggttc tccgctgttt atggaaatcg aaccacgttt ctccatcgac
    13681 aagctgacca atactgacct tagcttcggt ccgttcaaag agtggtactt cgcgaacaac
    13741 tacatttacg acatgggtcg taataaagat ggtcgccaga gcacctggta catgggtctg
    13801 ggtaccgata tcgacactgg cctgccgatg agcctgtcca tgaacgtcta tgcgaaatac
    13861 cagtggcaga actatggcgc agcgaacgaa aacgagtggg acggttaccg tttcaaaatt
    13921 aaatactttg tgccgattac cgatctgtgg ggcggtcagc tgagctacat cggcttcacc
    13981 aacttcgact ggggttccga tttaggggat gacagcggta acgcaatcaa cggtattaag
    14041 acccgtacta ataactctat cgcttccagc catattctgg ctctgaacta cgatcactgg
    14101 cactactctg tcgtagctcg ttactggcac gacggtggtc agtggaacga cgatgcagaa
    14161 ctgaacttcg gcaacggcaa cttcaacgtt cgctctaccg gctggggtgg ttacctggta
    14221 gtaggttaca acttctgatt atgaaaatgc cgggatttat tcccggcatt tctgattgtt
    14281 aacaacgtgt gctttgttca tgccggatgc ggcgtaaacg ctttgtccgg cccacaaaag
    14341 cacgtaaatt caatatattg ctgaaataac gtaggcctga taagcgtagc gcatcaggcc
    14401 attttacgtt ttaatcactt cttcttgttc atcatcgctt tcaggtcagc gaatgggttg
    14461 tacttcgctt caccgacatc tttctgcgca tcttcccctg cgataacggt cgtaccatac
    14521 tgatccgctt cggtatattt cgaatgctca tgatcgtggc aatagagaca caacaattcc
    14581 cagttactgc catcttccgg gttattggta tggtcgtgat caatgtggtg aacggtaagt
    14641 tcacgcaggt tagaataaac aaactcgcgg gaacagcgac cgcagaccca cggatagatt
    14701 tttaatgctt tttcgcgata gccgctttct aaccgtgcat agttttttgg gatgatagcc
    14761 atgaaaagtt acctggatga aatgtagggt tacgcgcagt ttcccaagat gcggggattt
    14821 tcgcaatctc catacatcaa aaaaaatccc gatcttctga ccgggatttt caacatcaac
    14881 gggaacttga ttaaggcaga attgacggct gatccgcccc ttctttttcc actttctgct
    14941 gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa cgcagatgct acgtagatgg
    15001 aagatgcagt gccgatggaa acaccgataa gcatggtcag cgagaagcct tccagtaccg
    15061 gaccaccgaa gagatacagc atcaggataa ccatcaaggt agtaccggat gtgatcaagg
    15121 tacggtgcag cgtctgggtc aaggacacgt taaagatttc gtaaggcgta ccgcgacgga
    15181 tcttgcggaa gttttcacga attcggtccg ataccacgat actgtcgtta agcgagtaac
    15241 cgataaccga catcaacgat gccacaatgg tcaggtcaat ctcgatatgg aataacgaca
    15301 aaatacccag cgtaatgata acgtcgtgcg tcagcgcaat aaccacccct gccgccagtc
    15361 gccactcaaa gcggaaacct acgtacacga ggatagacag cagcgctgcc atcaacgcca
    15421 tcgcaccggt ttgcgcaagg tcagccccca cgctcggacc gacgaactca atacgcttca
    15481 ctgctgcatt ctgattggtg gattcgttaa tcaccttcag aacttggctg cccagcacct
    15541 gaccgccggt ttcgccttca gcaggcggca tacggaccat gatgtcgtgg ctgctaccaa
    15601 agttttgcag cattggctct tcaaaaccgg ctttttgcaa tgcatcacgc attacgtcaa
    15661 tttcagccgg tttttcgagc gtaatttcga taaccgtacc accggtgaaa tccagccccc
    15721 agttaaagcc gcgcacgccc ataataacga tagcagcgat taacagcaga ccagagatgc
    15781 cgaaagccca gtagtcccag cgcataaagt catagacttt acggccgtgg tttagttgtt
    15841 caacagtata ttcctgtgcc acatcgcact cctcagattg acagcttctt gacgcgcttg
    15901 ccgccatata gcaggtttac gatggcacgc gtaccgacaa tcgcggtaaa catcgacgtc
    15961 gccacaccga taccggtagt aatcgcgaac cctttaattg ccccggtacc cactgcgtac
    16021 aggatgatga ctttaatcag cgtggtgatg ttcgcatcga agatagaact gaatgcgcca
    16081 cgataacctt catcaattgc ctgttgaaca gtacgtccgt tgctcaactc ttctttaata
    16141 cgttcgttga tcagtacgtt cgcatcgacc gctaccgcaa gggttaagac gatacccgcg
    16201 atacctggca tactcagtgt tgcgcctggc aacagcgaca taataccgac gatcaagatc
    16261 aagttggcaa tcagagcact ggtcgcaatc agaccaaact tcttatagaa gatgatcatg
    16321 aacagaatag aaaccagcag accggcaagg caagcctcca gcccctgctc aatgttctgc
    16381 atacccaggg tcgggccaat tgtacgttct tcaacaatct gaatcggcgc gatcaacgca
    16441 ccggcacgca gcagcagtga cagctgacgg gcttcgttcg ggttgttgat gccggtgata
    16501 cggaagctgt tacccagacg agactggatg ttggcgatgt taatcacctc ttcctgtttc
    16561 accagaaccg cacgaccatt tgcatctttc ttaccgctgt ctttgtactc cacaaacagg
    16621 gttgccatcg gtttgccgat gttgtccttg gtgaagttag acataatgtt gccaccagcg
    16681 ctatcgagcg agatgttaac ctgcggctgg ttgtattcgt cctggctgga agtggagtcg
    16741 gtgatatggt caccagtcag aattacgcgt ttgtacagca caaccggctg accttcgcgg
    16801 gtctgtttca cttcagagtc gcccggtacg cgaccggatg ccgcagcggc ctgatcaaca
    16861 ttggtgttta ccagacggaa ttccagcgtt gccgtcgcgc ccagaatctc tttcgcacgc
    16921 gcagtgtcct gaataccggg cagttcaaca acgatacggt cagcaccctg acgctgaacc
    16981 accggctccg ccacgccaag ttggtttaca cggttacgca ggatattaat gttctgctgc
    17041 accgcatatt cacgcgcttc actcagacga gcatcgctca ttaccgcacg cagctggttg
    17101 ctgccctggc tgctaatcac caggtccgga tggcgcttgc tcagatacgc aatggcttca
    17161 tcacgagctt tagcatcgcg gaaagtgatg ctcaggccgt agttgttttc tttacgaaca
    17221 gtggtatacg ggatgccctt ttcgcgcagg tcactgcgta ggctatcgat attttgttcc
    17281 tggagtttgc caagcgcggt gtccatatcc acttccatca ggaagtgaac gccgccacgc
    17341 aggtcaaggc cgagcttcat cggctcagca tgaatagctg ccagccagcg cggcgttgcc
    17401 ggggcaaggt taagcgccac gacgtatttg tcacccataa cgcccattaa tgcttcacga
    17461 gcgcgcaact gggtgtcagt ggagtcgaag cgcgcaagaa tagcgccctc ttccagtgcc
    17521 acagacttag cagttatttt ttcttcttgt aacgttttct ggacctggat cagcgtttgc
    17581 tcactggcgg cgactccgcg cgcaccagtg atctgaacag ccggatcctc accaaacagg
    17641 ttgggaagcg catacagcag accgatgaca atcaccacga tcagcatgac gtacttccac
    17701 aaaggataac ggtttaacac ggcaattccc ttagggaaaa attttaatta cagcgccttc
    17761 atggtgcctt tcggcaggac ggcagctacg aagtcacgtt taataactac ttcagtggtg
    17821 tcattcagcg cgatagcaat gtagccgttt tccgctactt tggttacgcg accaaccagg
    17881 ccaccgttcg tcagaacttc atcacctttg gcaatggagt ccatcagctt tttgtgttct
    17941 ttggtgcgct tctgctgtgg acgcaggatc atgaaataga aaatcagacc gaataccacc
    18001 agcatcaaaa tcaaagacat cgggctacct tgcgccggtg cacccgttgc cgctaccgca
    18061 tcagaaataa aaaagctcat taaatttccc tcattattaa tattaatcaa cgttcaaagg
    18121 tggtacttct cgcccctgac gctggtaaaa atcagttacg aagctctcta atttaccctc
    18181 ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag taacgaaggt tatgaatggt
    18241 gttgagtcgc gcgcctaata tttcgttgca acggtcgaga tgatgcaagt aagcgcgtga
    18301 ataattgcga caggtgtagc aatcacactc aggatcgagt gggccagtat cgctcttata
    18361 tttcgcattg cggattttca ccacgccatc ggtcacgaac aaatgaccat ttcgggcgtt
    18421 gcgggttggc attacgcagt caaacatatc gataccgcga cgtacgcctt caagcaggtc
    18481 ttccggttta ccaacgccca tcaggtaacg cggtttgtct gccggaattt gcggacacac
    18541 atgctccaga atacggtgca tatctgcttt cggctcaccc acagccagac cgccgacagc
    18601 gtagccatca aagccgatat ctaccagacc tttaacagat atatcacgta aatcttcgta
    18661 aatactgccc tgaattatac caaataacgc atttttgtta ccaagactgt caaaacgctc
    18721 acggctacgc ttcgcccaac gcagagacat ctccatagag cgttttgcgt aatcccagtc
    18781 agcaggatac ggcgtacact catcaaagat catgacgata tccgaaccaa gatcgtactg
    18841 aatctccatc gacttttccg ggtcgaggaa aatcgggtcg ccgttgatcg ggttacggaa
    18901 gtgaacgccc tgttcggtga ttttacgaat atcaccaagg ctgaagacct ggaagccgcc
    18961 ggagtcggta agaatcggtc ctttccactg cataaaatcg tgcagatcgc cgtgcagttt
    19021 catgatttcc tggcccgggc gcagccacag gtggaaggtg ttgccgagga taatttgcgc
    19081 gccagtggct tcaacttctt ccggcgtcat cccttttacg gtgccgtagg tgccaacagg
    19141 cataaaacaa ggcgtttcca ctacgccacg atcaaagacc aggcggccac ggcgtgcgcg
    19201 accgtcggtg gtgtccagtt caaatttcat tttttctcca ctacgtcaga aaaacagtcc
    19261 aacgtttaaa ccagcgccgc ggaattactc cccgacgcgc tcattaattg cctgcggatt
    19321 gtacgtgata aacatcgcat caccgtaact aaaaaagcga tatttctctt ctaccgccgc
    19381 tttataggca ttcatggtgt gttgataacc ggcaaaggcc gaaaccagca taatcagcgt
    19441 cgactctggc aagtggaagt tcgtcaccag cgcatcgacc actttgtact ggaagcccgg
    19501 atagataaag atttgggtat cgtcaaagaa cggttcgata agatcgtttt ttgccgcctg
    19561 tgccgcgctt tccagcgaac gtactgaagt ggtgccaacc gcaatcaccc ggttaccgcg
    19621 cgctttcgcc gccagtaccg cgtctaccac atcctgcggt acttcagcgt attccgagtg
    19681 catgatgtga tcttcaatgg tgtcgacgcg caccggctgg aaggtgcccg caccaacgtg
    19741 caacgtcaca aacgccatct ccacgccttt ggcgcgtaat ttttccagca aaggctcgtc
    19801 aaaatgcaga ccagcggtcg gggctgcaac cgcgcccggt ttttcgctat aaacggtttg
    19861 ataaagttcg cggtcagcgt cttcgtccgg acgatcgata tacggcggca gcggcatatg
    19921 gccgatgctg ttgaggatat ccagtaccga gcgttcatca ttaaattcga cttcaaacag
    19981 tgcgccgtgg cgcgcggtca ttgttgcgtt gatactttcg tcatcgccca gcaacagttc
    20041 tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt tgtcgtcgag
    20101 catccgctca accagcactt caatcttgcc gccgctggct ttacgcccaa acagacgcgc
    20161 cgggatcacg cgggtattat taaaaaccag aagatcgccg gggttgagct tatcgagtaa
    20221 atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg tccagcgaca gtaaacgaca
    20281 gctactgcgt tcaggcatgg gatagtgggc aatcagggat tcgggcaatt caaaggagaa
    20341 atcggtaacg cgcatgacac tgactcagac taaaataaga gtcgggtagt ctagtgccgg
    20401 ggcgctctcc ctgcaacctt tacccccctt tggatagaat ataatcgatg aattttttag
    20461 ctcacctgca tttagcccat ctcgcggaaa gctcgctttc tggcaattta ctggctgatt
    20521 tcgtacgcgg aaatcctgaa gaaagttttc cgcccgacgt cgtggctggc attcatatgc
    20581 atcgacgtat tgactgacaa tctgccggaa gtccgcgaag cacaggagtg gtttcgtagt
    20641 gaaacgcgcc gcgttgcgcc tattacgctg gatgtcatgt gggatcactt tctttcccgc
    20701 cactggtcgc agctgtcgcc ggactttccg ctacaggaat ttgtctgtta tgcccgcgag
    20761 caagtgatga cgattttgcc ggactcaccg ccacgtttta tcaatctgaa caattacttg
    20821 tggtcagagc agtggctggt gcgctatcgc gatatggatt tcatccagaa cgtgttaaac
    20881 ggcatggcaa gccgccgccc acgtctggat gccctgcgtg actcctggta cgatttagac
    20941 gctcattatg acgccctcga aacccgcttc tggcagtttt atccgcggat gatggcgcag
    21001 gcgtcacaca aggcgttata aaacgttagt tcatccatac cgtagccgaa atggcaggca
    21061 aagccagaat cccgtcagtc agttgcccat gcccttcttt gcattgccat tgcacggcat
    21121 tgagaaacgg tgacgcgggt agcaccactt cactggcctc gccacggttg attgccacca
    21181 gtacacgttg ctgattcagc acgcggacaa ataccactac gttatcttcc gcatacagca
    21241 cctgacagcc gccacgacgc agcgcctgac ttttcttacg cagcacaatc attcgccggt
    21301 acagcgcgaa taacgccgta tcctgctttt ccacctgcca ggggaacggt ttacggcaaa
    21361 acggatcgtt tttaccatcc agtcccactt catcaccgta ataaatgcac ggtacaccag
    21421 gccaggtgaa cagccagacc accgccagcg gcaggcgcgc aatatcccga ccgagcagcg
    21481 ttttaaatcg cgcagtatcg tggctatcga gctgattaaa catacgtaat tgttgttgat
    21541 gagaaagccc tgcgcggtaa ttatccatcc aggccataca ggtttgggcg tcaatttgct
    21601 gcggatcata agagatgtcg gtattggcaa gaaatcccca caacgggaat gtgaagccac
    21661 gatagttcat ggcagcatct tccacatcgg cctgtaacca ttgccgtgca tcgccaaaat
    21721 gttcgccgac aatatacgct tccggctggg tttctttcgc cgcttcggtg atcccggcaa
    21781 cgtgctgcat attattgcgc gccccacccg cctcccccag catatgcacc acatccagcc
    21841 gccagccgtc catattccac ggcgctttca gccagtggcg gacaatactg tcttccccgc
    21901 gataaatttc attcaccaga ctttccgact gataatccag cttcgtcaag ctggcatagc
    21961 caagccagtc gagcgccgtg ccatcatcac taaacgagta ccagtcgcgc cagggcgatt
    22021 cagagttgtg acaagctccc cccgtgccaa gattgtgcct gtcaaaccag gcatgggaat
    22081 cgccactgtg gttaaacacg ccgtccagca ccagccgcat tcccagctgc tgcgtattgt
    22141 gtcgcaaacg cagcaacgcc ccatcaccgc caaactgcgg atcgacatgg cgataatcct
    22201 cggtatcgta tttatgtacg ctgggagctt taaacaccgg attgagatac agcgctgtca
    22261 cgccaagctt tttcagatac ggcagttttt cgcttatccc gtccagatcg ccgccataga
    22321 acgttgatcc gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag atgatctctt
    22381 gtccggctgc atgatggtaa tagacatgat cctgttcagc ttcacgagga agactacgcg
    22441 caaaacgatc agggaagatc tgataaaaaa tctgatccgc agcccattgt gggccgatat
    22501 caggtacatc gacggcaaac tgctccagtc gtgccggcgg cattcggctg aagccctgcg
    22561 gtgtaaacca acgctggcga tcgtgccaca gcagtttgaa actgtaacgc cgccggggtt
    22621 gtccgctgga gagatcaatc gccgcacgcc atgcggtgac gcctggctgc ggctgactgc
    22681 gctgcttatg cattggtact gacatttctt cgttatcgtg ttctgtacgc agcataatgc
    22741 gctgcggtgg gtcttcgccc gtcagccaca gtgtaatgag cagttgatct ttgctttgtt
    22801 taacaaatgg gggcaccggc aggtgccatg catttaacat cataattccc ctgtgatgaa
    22861 aatctgatca ccttgccata gcctaatgca ggatcactca tcatctgaag gatatttggg
    22921 ggaggaggac gggggcggat aactttcgga atagccggat caggatttcc tcctgaaccg
    22981 gcttacagaa cgcaagtatt attggttttc agccagctga cgatcgtggc ggcgtttaaa
    23041 catccagcca atcaacagca caacaatcca cgcgaaaccg acatacagcg agatacgcgt
    23101 atccgggtga taaccaatca acccgataat aaagagcagg aaaatcagcc cgccgatggt
    23161 cgttgctacc ccacccggca ctttaaattt cagcgcctta acttcttctg gcggcaaacg
    23221 gcgacggaag gcgatttgcg acagcaggat cataatccac acccataccg tggcgaaggt
    23281 tgccagcgaa gcgatcacca ggaagacgtt ttccggcatg atgtagttca gatacaccgc
    23341 aaacagcagc gcggtagtca tcaccagcac cgtaacccac ggaataccgc gacgcgacgt
    23401 tttgctgaaa attttcggtg cgctgccctg ctctgccata ccgtggagca tacggcctac
    23461 gccaaatacg tcactgttaa ttgccgacag cgaagcggtc agcacaacaa agttaagaat
    23521 gctggcggca aaggtaatgc ccatatgctg gaacgtcagt acgaacgggc taccggcagt
    23581 gccaacctga ttccacgggt agatagacat aatgacgaac agcgtaccaa cgtagaacac
    23641 cagaatacgc atcggcacgg agttaatcgc acgcggaatc gatttctcag gatctttcgc
    23701 ttcaccggcg gtaatcccga taatttcgat cccaccgtaa gcaaacatca ccatttgcaa
    23761 cgacattacc atgccaagcc agccgttact gaagaagccg ccgttgctcc acaggttatg
    23821 aataccggtc ggttgcccgc cgttgccaat cccccagatg atgatgccga aaccggcgac
    23881 aatcatgatg atgatggtgg cgactttaaa gaacgagaac cagaattcca gctcaccgaa
    23941 gaccttcacg ctcatcaggt ttacggcgca aatgatcagc accacgctca gtacccaaat
    24001 ccagtgcggc accgtcggga acaagacacc catatagata ccaaaagcgg tcacatcggc
    24061 gatggcgaca ataaggattt caaaacagta ggtccatccg gtaatgtaac ctgccagcgg
    24121 gccaaggttt tcctgcgcat aacgcgagaa agagctggcg gccgggttat gtaccgacat
    24181 ttcccccagc gcacgcataa tgatatacgc cgcgatacca ccgataatat aggccaacaa
    24241 cacgctcgga ccggccattt tgatggcgtc tgccgaaccg taaaacagcc cggtgccaat
    24301 tgctgaaccc agtgccataa agcgtatgtg gcgggtactt agcccacgct ttagcttatt
    24361 cttactttcc atcaattcct aacccatcaa cacaataaaa aaccacgggg catcgcagcc
    24421 ccgtggttaa aacaaatgtt cagtgattta gtgagcgctg gaggtcacct gacgacctgc
    24481 cgcgcgatcc cagataatgg ccagaaccac catcaccact gttggcatta accacgccag
    24541 accttgttcg gccagcggta aacgctgcgc ccaggacggt aagatatcgc tgaatgcaga
    24601 tgctttgatc ccgtcgagaa taccaaaaag caggctgata aacatcggcg gagcaatcac
    24661 gcgggacgaa ttatgccacc atgagcgtgt aaaacttaat acaaccagtg cgatacacgg
    24721 cggataaata gcggtcagca ccggtacgga gatctggatc agctggctta agccgaggtt
    24781 agacaccacc atcgagaagc cgccgaggat aaacaccagc gtacgataag agagcggtac
    24841 gtactgggca aagaattctg cacaagcaca ggtcaggcca accgccgtga ccagacaggc
    24901 gatgaagatt aacgccgcca gcaggaagct accgccgccg ccaaaggtgt gctgaacgta
    24961 agcatgcaga atagcagcgc cgtttgcaga ctgatcgacc agcgacgcgc tgtctgaacc
    25021 cagacagaac agcgccaggt acagcaaagt cagaccaaca cccgccatca ggccagccca
    25081 gacggtataa cgggtcagca gacgcgcttc ggtaacgcca cgagaacgcg ccgcgttaac
    25141 aataacgata ccaaacacca ttgcgcccag cgtatccatg gtcagatagc cgttaacgaa
    25201 gccgttagaa aatgcagcgt tttgataagc ctcagtcgcc gtgctgatag aacccgccgg
    25261 ccagacaatc gcgacaacag acaggatgac cagcgcgata attttcagcg gcgcaaggaa
    25321 gttgcccacg gtatccagca gcttgcccgg atagagcgaa accagaataa cgatagcgaa
    25381 atagaccagg ctgtagataa acagcggcag cgcggaatca cccgtcagcg gcgcaatccc
    25441 cacttcaaag gaaacggtag ctgtacgcgg cgtagcgaaa agcggcccca ccgccaggta
    25501 acaaaccgtt gccagcagta cgccagcgac tttaccgatt ggcgtgctga ggctgtcaac
    25561 accgccgcca acttttgcca gcgccactac cgttaatacc ggcaggccaa cggcagtaat
    25621 gaggaagccg aatgccgcag tccagacgtg ttcgcctgcc tgtaagccga ccattggagg
    25681 gaaaataatg ttacctgcgc cgacgaacaa cgcaaatgtc ataaagccca gagcgatgat
    25741 atcgcgcgat cttaattgat gggtcataaa tcatactgcc tgtggatgtg gtgttgtaac
    25801 gttaaatttt tgtgccaatc cctggggatg atacagcggt atatttaaac aattttagcg
    25861 ggaaatactc ccaaccaggc gcggcgaaga atagtttttc gatttaccgc gttaatgcag
    25921 tcagtatgac agcacctgaa gcgcaattta aacgcttata acgttttaaa gcaaggaggg
    25981 atctaaaaac cagaggtata tagcgataag aaatcaccaa ccagtataaa acactatttc
    26041 cgtgaaaaag acatggctaa tcatgcgaac aaaaaatcac caaccgttgt aaaatctaac
    26101 gcccgtattt tgactgcaaa gaaaataagc cagcatcggc tggcttatgg caataaaaga
    26161 tgacaaaggc ggattaatcg ctgtttttgg caattaaacg ttccgggata acaaaactga
    26221 aacgtgttcc ttttcctact gtactctcaa tattcaggcg actttcgtga tgattcaccg
    26281 catgtttcac gatcgctaac cctaatccgc taccgccggt ttgccgggaa cgcgctttat
    26341 caacgcgata aaaacgctcg gtcaggcgcg gaatatgctc cggtgcaatg cccggtccgt
    26401 tatcttcaac gctaaattcg gcaccgtgcg gcactcgctg ccagcgtacg gtgatatgcg
    26461 tgccttccgg cgtatgattc acggcgttat agaccaggtt cgaaatcgca ctgcgtagct
    26521 gatcttcgtt gccagacacc ttgaggccgt tatctatctc aaaggtaaat gtctgttttt
    26581 tctgactcag agtctgagcc tcgcgctcaa caacgcgcag catcatcggc acatcaacct
    26641 tttcattgag caattgcgtc ggcgcggctt caattttcga cagcgttagc aactgcttca
    26701 ccagcccttc catccgctgg gtctgctcgc gcatggtgtg caacgctttt tcgcgtaccg
    26761 cgccttccag cggctgctca tccatcatct ccaggtaacc ctgtaacacg gtcaatggcg
    26821 tacgtaactc atggctcacg ttggcaaaaa agttacgccg cgccccttcc agttgatgca
    26881 tttgcgtgac atcacgcgcc accatcagca actgtttgtg ggtataaggc atgacgcgaa
    26941 tttccagatg ccgcccggtg ttgagcacca gattgagcgg gcgagaaaaa tcacgcgttt
    27001 tcagatattg cgtaaactcc gggtaacgca gtaggttaag gatgttctgc ccgttatctt
    27061 ccggccagcg caaaccaaga atttgttgcg ccagaccgtt acaccagaaa ataccgccct
    27121 cttccgtggt cagcaccacc gcgtcgggca gcgactccgc gccgctacga aagcgtttaa
    27181 tcagattgcc cagttcacgg cggcgttttt tatttcgcag ctgcatctgg tgtaagccgt
    27241 atagtagcgg ttcccagcta ccacgccccg gtggcggggt catactgcga tccacccaca
    27301 gccaccatga aaggcgcaat aaattccaga aatgccagat aagcagtcct gttaccgatg
    27361 ccagcaaaaa ccagggcagg taaccaaaaa atgcacccag gatgaaagcc gggaggcagc
    27421 aaagtagcag ctccagcacc agccttttcc acgacagccg ttccagcacg taagatactc
    27481 cagttaagaa atcataagcc ctgctctgcg tccgatgagc aaggcgttaa aagcgggttg
    27541 aaaaacgata tcctgtaccg cgcacggtct gcaccatgcg gtcatgcccg ccgggctcca
    27601 gtgctttacg caggcgacga atgtggacat cgaccgtgcg gtcttccaca tacacgttag
    27661 ttccccagac gtggtttaac agctgctcgc ggctgtacac gcgctcagga tgcgtcataa
    27721 aaaagtgcag cagtttaaat tctgtcggcc ccatctccag cggctcttcg cccgccatca
    27781 ctcggtgaga tgtcgggttg agacttaatc cctgcatctt aatcacctct tccaccgcca
    27841 ttggcgaaat acggcgcatt accgctttga ttcgcgccac cagctccttc ggcgaaaatg
    27901 gcttggtgat atagtcatcc gcgccggttt caaggccgcg cacgcgatct tcttcttccc
    27961 ctctggcggt caacatcacc actggaatat cccgggtcat cgactcgcgc ttgaggtgtt
    28021 tgatgaactg gataccggag ccgccaggta acatccagtc aaggagaatt aaatccggcc
    28081 agggttcatt cagttgattc acagcactgt cataatcttc cgcttcgacc ggctgaaagc
    28141 cattttgttc gagcacgaag cagaccattt cgcgaattgg agcttcatct tctacgacca
    28201 gaatacgtct cgccatgatt tgccctgttg taataaatag gttgcgatca ttaatgcgac
    28261 gtcattatgc gtcagattta tgacagattt atgaaaagct cgtcgcacat atcttcaggt
    28321 tattgatttc cgtggcgcag aaaaaagcaa atggcacatc tgtttgggta taatcgcgac
    28381 catgcttttt cgccagggaa ccgttatgcg catccttcac acctcagact ggcatctcgg
    28441 ccagaacttc tacagtaaaa gccgcgaagc tgaacatcag gcttttcttg actggctgct
    28501 ggagacagca caaacccatc aggtggatgc gattattgtt gctggtgatg ttttcgatac
    28561 cggctcgccg cccagttacg cccgcacgtt atacaaccgt tttgttgtca atttacagca
    28621 aactggctgt catctggtgg tactggcagg aaaccatgac tcggtcgcca cgctgaatga
    28681 atcgcgcgat atcatggcgt tcctcaatac aaccgtggtc gccagcgccg gacatgcgcc
    28741 gcaaatcttg cctcgtcgcg acgggacgcc aggcgcagtg ctgtgcccca ttccgttttt
    28801 acgcccgcgt gacattattt ccagccaggc ggggcttaac ggtattgaaa aacagcagca
    28861 tttactggca gcgattaccg attattacca acaacactat gccgatgcct gcaaactgcg
    28921 cggcgatcag cctctgccca tcatcgccac gggacattta acgaccgtgg gttccagtaa
    28981 aagtgacgcc gtgcgtgaca tttatattgg cacgctggac gcgtttccgg cacaaaactt
    29041 tccaccagcc gactacatcg cgctcgggca tattcaccgc gcacagatta ttggcggcat
    29101 ggaacatgtt cgctattgcg gttcccccat tccactgagt tttgatgaat gcggtaagag
    29161 taaatatgtc catctggtga cattttcaaa cggcaaatta gagagcgtgg aaaacctgaa
    29221 cgtaccggta actcaaccca tggcggtgct gaaaggcgat ctggcgtcga ttaccgcaca
    29281 gctggaacag tggcgcgatg tatcgcagga gccacctgtc tggctggata tcgaaatcac
    29341 tactgatgag tatctgcatg atattcagcg caaaatccag gcattaaccg aatcattgcc
    29401 tgtcgaagta ttgctggtac gtcggagtcg tgaacagcgc gagcgtgtgt tagccagcca
    29461 acagcgtgaa accctcagcg aactcagcgt cgaagaggtg ttcaatcgcc gtctggcact
    29521 ggaagacctg gatgaatcgc agcagcaacg tctgcagcat cttttcacca cgacgttgcg
    29581 taccctcgcc ggagaacacg aagcatgaaa attctcagcc tgcgcctgaa aaacctgaac
    29641 tcattaaaag gcgaatggaa gattgatttc acccgcgagc cgttcgccag caacgggctg
    29701 tttgccatta ccggcccaac cggtgcgggg aaaaccaccc tgctggacgc catttgtctg
    29761 gcgctgtatc acgaaactcc gcgtctctct aacgtttcac aatcgcaaaa tgatctcatg
    29821 acccgcgata ccgctgaatg tctggcggag gtggagtttg aagtgaaagg tgaagcgtat
    29881 cgcgcgttct ggagccagaa tcgggcgcgt aaccagccgg acggtaattt gcaggtgcct
    29941 cgcgtagagc tggcgcgctg cgccgacggc aaaattctcg ccgacaaagt gaaagataag
    30001 ctggaactga cagcgacgtt aaccgggctg gattacgggc gcttcacccg ttcgatgctg
    30061 ctttcgcagg ggcaatttgc tgccttcctg aatgccaaac ccaaagaacg cgcggaattg
    30121 ctcgaggagt taaccggcac tgaaatctac gggcaaatct cggcgatggt ttttgagcag
    30181 cacaaatcgg cccgcactga gctggagaag ctgcaagcgc aggccagcgg cgtcacgttg
    30241 ctcacgccgg aacaagtgca atcgctgaca gcgagtttgc aggtacttac tgacgaagaa
    30301 aaacagttaa ttaccgcgca gcagcaagaa caacaatcgc taaactggtt aacgcgtcag
    30361 gacgaattgc agcaagaagc cagccgccgt caacaggcct tgcaacaggc gttagccgaa
    30421 gaagaaaaag cgcaacctca actggcggcg cttagtctgg cacaaccggc acgaaatctt
    30481 cgtccacact gggaacgcat cgcagaacac agcgcggcgc tggcgcatat tcgccagcag
    30541 attgaagaag taaatactcg cttacagagc acaatggcgc ttcgcgcgag cattcgccac
    30601 cacgcggcga agcagtcagc agaattacag cagcagcaac aaagcctgaa tacctggtta
    30661 caggaacacg accgcttccg tcagtggaac aacgaactgg cgggttggcg tgcgcagttc
    30721 tcccaacaaa ccagcgatcg cgagcatctg cggcaatggc agcaacagtt aacccatgct
    30781 gagcaaaaac ttaatgcgct tgcggcgatc acgttgacgt taaccgccga tgaagttgct
    30841 accgccctgg cgcaacatgc cgagcaacgc ccactgcgtc agcgcctggt cgcgctgcat
    30901 gggcagattg ttccccaaca aaaacgtctg gcgcagttaa tggtcactat ccagaatgtc
    30961 actctggaac agacgcaacg taatgtcgca cttaacgaaa tgcgccagcg ttataaagaa
    31021 aagacgcagc aacttgccga tgtgaaaacc atttgcgagc aggaagcgcg catcaaaacg
    31081 ctggaagccc agcgtgcaca gttacaggcg ggtcagcctt gtccactttg tggttccacc
    31141 agccacccgg cggtcgaggc gtatcaggcg ctggagcctg gtgttaatca gtctcgatta
    31201 ctggcgctgg aaaacgaagt taaaaagctc ggcgaagaag gtgcggcgct gcgcgggcaa
    31261 ctggatgcat taacgaagca gcttcagcgc gatgaaaacg aagcgcaaag cctccgacaa
    31321 gatgagcaag cacttactca acaatggcaa gccgtcacgg ccagcctcaa tatcaccttg
    31381 cagccacagg acgatattca accgtggctg gatgcacaag atgagcacga acgccagctg
    31441 cggttactca gccaacgaca tgaattacaa gggcagattg ccgcgcataa tcagcaaatt
    31501 atccagtatc aacagcaaat tgaacaacgt cagcagcagc tcttaacggc attggcgggt
    31561 tatgcactga cattgccaca ggaagatgaa gaagagagct ggttggcgac acgtcagcaa
    31621 gaagcgcaga gctggcagca acgccagaac gaattaaccg cgctgcaaaa ccgtattcag
    31681 cagctgacac cgattctgga aacgttgccg caaagtgatg atctcccgca cagcgaagaa
    31741 actgtggcgc tggataactg gcggcaggtt catgaacaat gtctcgcatt acacagccag
    31801 cagcagacgt tacagcaaca ggatgttctg gcggcgcaaa gtctgcaaaa agcccaagcg
    31861 cagtttgaca ccgcgctaca ggccagcgtc tttgacgatc agcaggcgtt ccttgcggcg
    31921 ctaatggatg aacaaacact aacgcagctg gaacagctca agcagaatct ggaaaaccag
    31981 cgccgtcagg cgcaaacgct ggtcactcag acagcagaaa cgctggcaca gcatcaacaa
    32041 caccgacctg acgggttggc tctcactgtg acggtggagc agattcagca agagttagcg
    32101 caaactcacc aaaagttgcg tgaaaacacc acgagtcaag gcgagattcg ccagcagctg
    32161 aagcaggatg cagataaccg tcagcaacaa caaaccttaa tgcagcaaat tgctcaaatg
    32221 acgcagcagg ttgaggactg gggatatctg aattcgctaa taggttccaa agagggcgat
    32281 aaattccgca agtttgccca ggggctgacg ctggataatt tagtccatct cgctaatcag
    32341 caacttaccc ggctgcacgt gcgctatctg ttacagcgca aagccagcga ggcgctggaa
    32401 gtcgaggttg ttgatacctg gcaggcagat gcggtacgcg atacccgtac cctttccggc
    32461 ggcgaaagtt tcctcgttag tctggcgctg gcgctggcgc tttcggatct ggtcagccat
    32521 aaaacacgta ttgactcgct gttccttgat gaaggttttg gcacgctgga tagcgaaacg
    32581 ctggataccg cccttgatgc gctggatgcc ctgaacgcca gtggcaaaac catcggtgtg
    32641 attagccacg tagaagcgat gaaagagcgt attccggtgc agatcaaagt gaaaaagatc
    32701 aacggcctgg gctacagcaa actggaaagt acgtttgcag tgaaataact attcagcagg
    32761 ataatgaata cagaggggtg aattatctct tggccttgct ggtcgttatc ctgcaagcta
    32821 tcactttatt ggctacggtg attggtagcc gttctggtgg ttgtgatggt ggtatgaaaa
    32881 aagtcatttt atctttggct ctgggcacgt ttggtttggg gatggccgaa tttggcatta
    32941 tgagcgtgct cacggagctg gcgcataacg taggaatttc gattcctgct gccgggcata
    33001 tgatctcgta ttatgcactg gtggtggtgg tcggtgcgcc aatcatcgca ctcttttcca
    33061 gccgctactc actcaaacat atcttgttgt ttctggtggc gttgtgcgtc attggcaacg
    33121 ccatgttcac gctctcttcg tcttacctga tgctcgccat tggtcggctg gtatccggct
    33181 ttccgcatgg cgcatttttt ggcgtcggag cgatcgtgtt atcaaaaatt atcaaacccg
    33241 gaaaagtcac cgccgccgtg gcggggatgg tttccgggat gacagtcgcc aatttgctgg
    33301 gcattccgct gggaacgtat ttaagtcagg aatgctggcg ttacaccttt ttattgatcg
    33361 ctgtttttaa tattgcggtg atggcatcgg tctatttttg ggtgccagat attcgcgacg
    33421 aggcgaaagg aaatctgcgc gaacaatttc actttttgcg cagcccggcc ccgtggttaa
    33481 ttttcgccgc cacgatgttt ggcaacgcag gtgtgtttgc ctggttcagc tacgtaaagc
    33541 catacatgat gtttatttcc ggtttttcgg aaacggcgat gacctttatt atgatgttag
    33601 ttgggctagg gatggtgctg ggaaatatgc taagtggcag gatttcagga cgttattcac
    33661 cactgcgcat tgcagcagtg actgacttta tcattgtact ggcactgctg atgctctttt
    33721 tctgcggcgg catgaaaaca acgtcgctta tttttgcttt tatttgttgc gcgggattat
    33781 ttgccctttc agcacctctg caaatattgt tactgcaaaa tgccaaagga ggagagttat
    33841 taggtgccgc aggtgggcaa atagcgttta acctcggtag cgccgtcggc gcatattgcg
    33901 gaggtatgat gctgacgctg gggctggcat ataattacgt ggcgctgcct gccgccctgc
    33961 tttcgtttgc tgcgatgtca tcgttgctgc tgtatggtcg ctataagcgc cagcaagcgg
    34021 cggatagtcc ggtgctggcg aaaccactgg ggtagattac tgtcacggtg cgcgttgctt
    34081 attgccggat gcggcgtgaa cgccttatcc gccctacgcg gttctggcac attttgtagg
    34141 cctgataaga cgcggcaagc gtcgcatcag gcatgatgcg ccaattgcct acgtttttta
    34201 ctcttgtggc cataaccacg cagcgccgcg tacgccgctg gaatcaccgt gcttcgcctt
    34261 actcaccggc gtttcacatt cgccgccgaa gacaaattgt ttaatcaact gcccaaccgt
    34321 ttgatataaa cggtctacat tgctcatccc gccccccagg acaatcacat ccggatcgag
    34381 aatattcacg acatgtgcca gcgattttgc cagccgcagc tcgtagcgac gcaatgccag
    34441 ttccgctacc ggatcgcttt cttcaaccag gcggataatt tcactgcctt tcagcgcatg
    34501 tccgctcaaa cgacgataat ccgtcgcgaa tcccgtgccc gaaataaagg tttcaataca
    34561 accttgttta ccgcaataac aagggacttc ctcgcgataa cgcagttcgt cttcgtccat
    34621 ccacggtagc ggattgtgtc cccactcacc tgccgtgcca ttgccgccga tatgcgtccg
    34681 cccattgaat gccacgcccg cgccgcatcc cgtgccgata atcacggcaa ataccgtctg
    34741 cgctcccgct gccgcgccat ctactgcttc tgaaaccgcc agacagttgg catcatttgc
    34801 cagccgcact tcccgctgca acctcgcgct taagtcttta tcgaatggct gaccgttgag
    34861 ccaggttgaa ttgacattct tcaccacacc ggtgtagggc gaaatcgaac ctggaatgcc
    34921 catacctacc gttccgcgct gccctgtcgc ctgctccgcc atatcaacca acgtggcgat
    34981 cgtttcaata gtctgccggt aatcatcacg cggcgtgggc agacgatggc ggtacaactg
    35041 ctcccctgca tcgcccagtg caatcacttc agttttggtg ccgcctaaat cgatacctat
    35101 acgcactgta ctctccttat ttttttcaat atcaatagcg tagagacgga caaccggatt
    35161 ggcaatgcaa ggccgccgac aattcgttat catgcccgct aaatttaacg acaaggccgt
    35221 ggaaattatc atgctgtggt tcaaaaattt aatggtttac cgtcttagcc gcgagatttc
    35281 gctgcgtgca gaagagatgg aaaaacagct agcctcgatg gcatttaccc catgcggcag
    35341 ccaggacatg gcgaagatgg gctgggttcc tccgatggga tcgcacagcg atgcgttaac
    35401 gcacgttgcc aatggtcaaa ttgttatctg cgcgcgcaaa gaagaaaaaa tcctcccgtc
    35461 tccggtgatt aaacaggcgc tggaagcgaa aatcgccaaa ctggaagcgg aacaggcgcg
    35521 taagctgaag aaaaccgaaa aagattcgct gaaagacgaa gtgctgcact ctctgctgcc
    35581 gcgtgctttc agccgtttta gccagacaat gatgtggctc gacacggtta acggtttgat
    35641 tatggtggac tgcgccagtg ccaaaaaagc ggaagatacg ctggcattac tgcgtaaaag
    35701 cctggggtcg ttaccggttg taccattgag catggaaaac ccgattgaac tgacgctgac
    35761 cgaatgggtt cgctccggta gtgcggcaca gggcttccag ctgcttgatg aagccgagct
    35821 gaaatcgttg ctggaagatg gcggcgtgat ccgcgcgaag aaacaagatc tgaccagcga
    35881 agagatcacc aatcacattg aagccggaaa agtggtgact aaactggcgc tcgactggca
    35941 gcagcgcatt cagtttgtga tgtgcgacga tggttcgctc aagcgtctta agttctgcga
    36001 cgagctgcgc gatcaaaacg aagatatcga ccgtgaagat ttcgcccagc gttttgatgc
    36061 cgatttcatc ctgatgactg gtgaactggc agcgttaatt caaaacctga ttgaaggatt
    36121 aggtggcgaa gcacaacgtt aattgctgat tttcctttaa tgccggatgc gacgcctgcc
    36181 gcgtcttatc cggcgtacga agccacacca ggcatataat tattcgctac ggcgagcaat
    36241 aatttttagc gcagcaatat tatgcgtttt acgccgtaac tgatctaatc gcctcatccc
    36301 tattcgcctg tcagcagcac gcagaatatt cactaacccc catttatcgc tggtaaattg
    36361 ttttgttaac agctccgctt ttggggtatc aatatattca cgcaataaat cagaaattga
    36421 acaaatatcc gtcaggtaag gataccagca agtttcaccg tgaaaattac gcactccagc
    36481 gttacctgca ataaagtctt tcgggtaatc ccagatcacg tttttatcca acgtgatcca
    36541 ataacgcggt aaatcggtac ttccaccatt ttggctgcgc atgggataac gggtacagtg
    36601 aatttgcagg ttgatcgttg gcgtcagtaa gtcatagatc tcccgctgta acttgctcca
    36661 tggacgttgt gtcattgttt ttcctcaagc cggtgacgcg cttcactgcg aattttccgc
    36721 gttgccccgg ttttcactaa atcacgtaac tgcgaaagat cgggttgttc gacaaataag
    36781 agtaatgatg gatcctcttt taaccacgcc gttttcacat cggcagccag ctgtggatta
    36841 ttaatagcca gcgaccgatc ctgaggctca gtcaatgtcg ttaacaacga cgccggggta
    36901 tgtgcgttgc gtaatgccgt ttggcgcaaa tgatcattgc gactttgcag caatgcacgc
    36961 caccattcat catctgattt atcttttatc gtagccagtt ccgcaaaaaa tacctggaga
    37021 tcttcactga tatttatatc aaccttgatg aattatgaat atatcggaaa ttgaaaaaat
    37081 cgcagcaaag gaaatattat tatgggttaa agagaggata tttcagaaaa atgccctccc
    37141 gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtag gttcggcaac
    37201 ttgaagatga aactcactgt gaccgggaac attaaacacc gaaccggctt catacacttg
    37261 ccagtcggtc gcgtcaggca gtaacacatt cagcgcgcca ctgattaccg tcatctcttc
    37321 cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacgc tggcgcgacc
    37381 agtgctgctg ctggaaaagc cgatcgattt cactttgccg gaaaagtact cattactttg
    37441 aagcataaac tggccctgtc tggtagttga acatctactt cactataggg gccagaggcg
    37501 ctggctgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat caatacgtta
    37561 gagagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg ttggtgaaat
    37621 cctaaacaat ccagcatgat gacatcagcc ccttttgcca gtaattcttt cccagcatca
    37681 atgatttttt gttctgaatc atgaatgggg ttacccaatg aaaataccgg cgatttctgc
    37741 aaaatttgcc atttttgcgc ctgaacgggc agcatctcct caaccggaac gataaccccc
    37801 acctgatgat cttcaacaat agaggaaacc agtggaggca atattcgcga cggctcaaga
    37861 aagatcgtat tacgcgcagt catactacta atgtttgctg tactcattaa taaaatgacg
    37921 tcataaccct gattatcgag cacttcaacc acaccttgca gatctcgctc cactttacga
    37981 cgcgaaacat gggccagctg gttgtcattt aataatgtga gaatggtgtc ttcgcctgct
    38041 tctggcgcgt actccgccat cacttcttca cgacttaact tccccagcag gctatgatgg
    38101 gaaatattat cttcgtcaat gtattccgtc aggagcggca aaacctcctg cataggtaca
    38161 atgccgatgg tcaggatcgc caacgacgca ctcattgttc atccacctta cttttctttc
    38221 gtcgttaatt accgggcaag tgtgaagcac cactgctgac acttacttgt gctacaaatg
    38281 acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat gattccagta
    38341 atggaatgtt attgtttaat attctaatta ttagaaaaac ataaattatg aaaaatgtga
    38401 cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc ggtcactctt
    38461 tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagccgtc ggatgttcac
    38521 tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt accgtctgtc
    38581 ctggctttcc tttttcaatc gtcactatat atgcttcacg aggataaggc ggtttcgttg
    38641 gcatcgttct tccttatttc acgggatgaa cgttaagtat aggcgctcta aaatcaacaa
    38701 ttgatcgtct gtgccagggc gctacgaatt tcagaaatca cctggctggg ttcgtttgtt
    38761 gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc cagcacttcc
    38821 tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc ttccggtgca
    38881 gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac gatcccgtta
    38941 ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt agcgataacg
    39001 gtggatgccg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa tcccgcccac
    39061 tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa ccactgatcg
    39121 gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt tgttttacca
    39181 cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc ccaataggtc
    39241 gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat accatcaaac tagtacaatt
    39301 tcgattgtaa agaaaaaatt ccacttaaag taaaaatctc aatacatccc ttactatacc
    39361 aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga ctacgttgca
    39421 cttccagcca ccacttctcc agctccgcgg caaaggcctg gcggtcacgt tgtgaaaggc
    39481 tatctggtcc gccggtctgg atcccactgg cacgtaaggt atccataaaa tcgcgcatcg
    39541 tcaggcgctc acgaatggtc gctggcgtgt aacgttcgcc gcgcgaatta agcgccgctg
    39601 cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc aaatcgcccg
    39661 cttcacactg ccggacaatt tcgttatcgg caacatcgaa acctgccgcg acgcgcagcg
    39721 tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt accagcggca
    39781 tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga cacgcgtcgg
    39841 catccaccca aattgtcata aagtcatcct ttgttaggta atcctctatt gtgtcgcgct
    39901 tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa caatgaattt
    39961 cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg caatatggga
    40021 aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca aatctgggta
    40081 tacaccccct ccccggataa agtcgccgcc ctgcatgacc agttcggcat caacgccgca
    40141 gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgctgccgt taaacctggc
    40201 atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc tctggtcgtt
    40261 tctattgctg caggtgtcac gctcgaccag cttgcccgcg cgctgggcca tgaccggaaa
    40321 attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac ctccgtaacg
    40381 ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt ccgctgcttt
    40441 ggcgaagcgg aagtaattgc tgagccgatg atccacccgg tggtcggtgt gagcggttct
    40501 tcgccagcct acgtatttat gtttatcgaa gcgatggccg acgccgccgt gctgggcggg
    40561 atgccacgcg cccaggcgta taaatttgcc gctcaggcgg taatgggttc cgcaaaaatg
    40621 gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc accgggaggc
    40681 accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc agtgatcgaa
    40741 gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg actttcgccg
    40801 gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg taaagcgcca
    40861 aatctgccga tttcaaccac tcacgatagt gactcatttg tgggttcagc ggcgcaaccc
    40921 ccacactaat ccgtaaagtt acctgtggcg tattcggcaa gcgtaacgtg tttagccctt
    40981 cacgcacccg taacatggcg gtaatggcgc tctcagctgg cgtaccggac atgattactg
    41041 caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta atttgtaact
    41101 gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg accccaggta tcgttgatgc
    41161 tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgc cgccgacagt
    41221 tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg cccgtcatgc
    41281 cgtcgcgagt actcatgacc tgcaacctgc gtttatgttc cgccagtttg gttgccgtct
    41341 ggtagctgac ccagccaaac atcagaggat aaacgacaat aatgggaagg gagagccacc
    41401 attccagcgg agcactattg aacgacacgg taatgcccgt aagctcgagg gtgacaaggc
    41461 aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct gcccccatca
    41521 aattcagaca cagaatcatc aacatagcgg tggaaggcag cacgtttacg cccattacgc
    41581 ctacccacat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc cggctaagcg
    41641 gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac gcccagccga
    41701 ccaacaccag ccaccaccag cccggcggcg ggtgcgaaac cagcgttgaa gcaatcggta
    41761 agaacatgcc agccaggcca accgcacggg gtagtctgac gcgacgggcg aagcgcagcc
    41821 cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc gccgccgctt
    41881 ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa ttttcccaaa
    41941 ttatagagac ggggctaaag gcagaagtat gaaatttcgg gtgagccact ttcgtagctc
    42001 acccgttagc act
//
LOCUS       DAZOZX010000022        40545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZOZX010000022 DAZOZX010000000
VERSION     DAZOZX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.022"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..957)
                     /locus_tag="ACHUPT_001142"
     CDS             complement(<1..957)
                     /locus_tag="ACHUPT_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR0818798.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1203..1829
                     /gene="gstB"
                     /locus_tag="ACHUPT_001143"
     CDS             1203..1829
                     /gene="gstB"
                     /locus_tag="ACHUPT_001143"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR0818799.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2940)
                     /gene="yliI"
                     /locus_tag="ACHUPT_001144"
                     /pseudo
     CDS             complement(1826..2940)
                     /gene="yliI"
                     /locus_tag="ACHUPT_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3051..3434)
                     /gene="bssR"
                     /locus_tag="ACHUPT_001145"
     CDS             complement(3051..3434)
                     /gene="bssR"
                     /locus_tag="ACHUPT_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0818800.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3647..4972
                     /gene="rimO"
                     /locus_tag="ACHUPT_001146"
     CDS             3647..4972
                     /gene="rimO"
                     /locus_tag="ACHUPT_001146"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0818801.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5019..6347)
                     /locus_tag="ACHUPT_001147"
     CDS             complement(5019..6347)
                     /locus_tag="ACHUPT_001147"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0818802.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6355..8697)
                     /locus_tag="ACHUPT_001148"
                     /pseudo
     CDS             complement(6355..8697)
                     /locus_tag="ACHUPT_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8875..9786)
                     /gene="gsiD"
                     /locus_tag="ACHUPT_001149"
     CDS             complement(8875..9786)
                     /gene="gsiD"
                     /locus_tag="ACHUPT_001149"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0818803.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9789..10709)
                     /gene="gsiC"
                     /locus_tag="ACHUPT_001150"
     CDS             complement(9789..10709)
                     /gene="gsiC"
                     /locus_tag="ACHUPT_001150"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0818804.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10727..12265)
                     /gene="gsiB"
                     /locus_tag="ACHUPT_001151"
     CDS             complement(10727..12265)
                     /gene="gsiB"
                     /locus_tag="ACHUPT_001151"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0818805.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12285..14156)
                     /gene="gsiA"
                     /locus_tag="ACHUPT_001152"
     CDS             complement(12285..14156)
                     /gene="gsiA"
                     /locus_tag="ACHUPT_001152"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0818806.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14143..15108)
                     /gene="iaaA"
                     /locus_tag="ACHUPT_001153"
     CDS             complement(14143..15108)
                     /gene="iaaA"
                     /locus_tag="ACHUPT_001153"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0818807.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15312..16547
                     /gene="moeA"
                     /locus_tag="ACHUPT_001154"
     CDS             15312..16547
                     /gene="moeA"
                     /locus_tag="ACHUPT_001154"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0818808.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16547..17296
                     /gene="moeB"
                     /locus_tag="ACHUPT_001155"
     CDS             16547..17296
                     /gene="moeB"
                     /locus_tag="ACHUPT_001155"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0818809.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17475..18137)
                     /gene="fsa"
                     /locus_tag="ACHUPT_001156"
     CDS             complement(17475..18137)
                     /gene="fsa"
                     /locus_tag="ACHUPT_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0818810.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18268..19167
                     /locus_tag="ACHUPT_001157"
     CDS             18268..19167
                     /locus_tag="ACHUPT_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0818811.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19173..21605
                     /locus_tag="ACHUPT_001158"
     CDS             19173..21605
                     /locus_tag="ACHUPT_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0818812.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21751..22566
                     /gene="ybiV"
                     /locus_tag="ACHUPT_001159"
     CDS             21751..22566
                     /gene="ybiV"
                     /locus_tag="ACHUPT_001159"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0818813.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22718..23983
                     /locus_tag="ACHUPT_001160"
     CDS             22718..23983
                     /locus_tag="ACHUPT_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0818814.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24224..25816)
                     /locus_tag="ACHUPT_001161"
     CDS             complement(24224..25816)
                     /locus_tag="ACHUPT_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0818815.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26035..26955
                     /gene="ldtB"
                     /locus_tag="ACHUPT_001162"
     CDS             26035..26955
                     /gene="ldtB"
                     /locus_tag="ACHUPT_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0818816.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27013..28131)
                     /locus_tag="ACHUPT_001163"
     CDS             complement(27013..28131)
                     /locus_tag="ACHUPT_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0818817.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28128..28595)
                     /gene="mntR"
                     /locus_tag="ACHUPT_001164"
     CDS             complement(28128..28595)
                     /gene="mntR"
                     /locus_tag="ACHUPT_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0818818.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28781..28909
                     /gene="mntS"
                     /locus_tag="ACHUPT_001165"
     CDS             28781..28909
                     /gene="mntS"
                     /locus_tag="ACHUPT_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0818819.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29181..30764
                     /gene="opgE"
                     /locus_tag="ACHUPT_001166"
     CDS             29181..30764
                     /gene="opgE"
                     /locus_tag="ACHUPT_001166"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0818820.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30812..31327)
                     /gene="ompX"
                     /locus_tag="ACHUPT_001167"
     CDS             complement(30812..31327)
                     /gene="ompX"
                     /locus_tag="ACHUPT_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0818821.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31380..31445)
                     /locus_tag="ACHUPT_001168"
     CDS             complement(31380..31445)
                     /locus_tag="ACHUPT_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818822.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31680..32567
                     /gene="rhtA"
                     /locus_tag="ACHUPT_001169"
     CDS             31680..32567
                     /gene="rhtA"
                     /locus_tag="ACHUPT_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0818823.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32866..33369
                     /gene="dps"
                     /locus_tag="ACHUPT_001170"
                     /gene_synonym="pexB"
     CDS             32866..33369
                     /gene="dps"
                     /locus_tag="ACHUPT_001170"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0818824.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33773..34519
                     /gene="glnH"
                     /locus_tag="ACHUPT_001171"
     CDS             33773..34519
                     /gene="glnH"
                     /locus_tag="ACHUPT_001171"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0818825.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34658..35317
                     /gene="glnP"
                     /locus_tag="ACHUPT_001172"
     CDS             34658..35317
                     /gene="glnP"
                     /locus_tag="ACHUPT_001172"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0818826.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35314..36036
                     /gene="glnQ"
                     /locus_tag="ACHUPT_001173"
     CDS             35314..36036
                     /gene="glnQ"
                     /locus_tag="ACHUPT_001173"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0818827.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36297..38521
                     /gene="ybiO"
                     /locus_tag="ACHUPT_001174"
                     /pseudo
     CDS             36297..38521
                     /gene="ybiO"
                     /locus_tag="ACHUPT_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38518..39444)
                     /gene="rlmF"
                     /locus_tag="ACHUPT_001175"
     CDS             complement(38518..39444)
                     /gene="rlmF"
                     /locus_tag="ACHUPT_001175"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0818828.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39720..39980
                     /gene="mcbA"
                     /locus_tag="ACHUPT_001176"
     CDS             39720..39980
                     /gene="mcbA"
                     /locus_tag="ACHUPT_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0818829.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40245..>40545
                     /locus_tag="ACHUPT_001177"
     CDS             40245..>40545
                     /locus_tag="ACHUPT_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0818830.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10097 a        10718 c         9867 g         9863 t
ORIGIN      
        1 ggagcccgct tcgcctgccc cgagattcac ttcgatctta tcaccaaacc agacgcgctg
       61 agtcacaaag gtggcatcag gtttaattgg cgtcacagtt tcaaagaagc ggaaacccca
      121 ggtcaataat ttctcagact cattaaaacg gatacggtca gttttcgccc ccagcactac
      181 ggagattaaa cgcatatcac cctgggtagc cgaagcaacc agattatatc ccgcacctgc
      241 cgtggttcct gtcttcatgc catcaacatt cagattgctg ctccacagca gacggttacg
      301 gttaggctga cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg cgtactcttc
      361 cggcacatcg tggatcaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg
      421 ccccggcgca tccaggccgt gcaccgtctg gaaggtagtg ttggtcagcc ccagtttttt
      481 ggcataacca ttcatcagac caataaagga ctcctggctc ccggcaacgt aatctgccag
      541 cgcaatacag gcgtcattac cggactggat aatcacacct ttgttcaagt ctgccaccga
      601 aacctgatcg cccggtttga ggaacattac cgatgaacca cgcagtgccg gatttcccgt
      661 cgcccaggca tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag
      721 cgcctgccca accacatagc tggtcatgat tttagtcagg ctcgcaggat ccagtttctc
      781 atccgcattg ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg
      841 cgcatccacg ctcggcgctt caacggtttg ttcggccgcg aatgccgttg gggcaaaaag
      901 gaataaaaat gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa
      961 tgccacccga gtatccattc aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagctccc aataaataat ttttgctgcc actgggggaa
     2041 tttatcgctg ttatagaagg ccatgccgct cacagcgggc gaatctttcc agtaaaaaac
     2101 aggttgctcg gtcccggcga cgatctcccc tttcgcttcc ggtatcttaa agcctgaata
     2161 gttgattccc caggttgcca gcggccagcc gtagtttttg cctttttgcg ggatattaat
     2221 ttcatcacca ccgcgcgggc catgttcatt cagccacagt gcattactcc acggattcat
     2281 cgccattcct tgcggattac gaatgccata agaccagatc tcggcgcgcg caccggattc
     2341 ctttataaaa ggattatcat ccgggatttc gccctggtcg gtcagacgca ccagtttgcc
     2401 ctgtaattta tccagatcct gcgccgtcgg gcgctgattg ttttcgccca gagcaataaa
     2461 aagataacct ttaccgtcga ataccagccg cccgccaaaa tggttgccgg tagacagttt
     2521 tggcatctgg cggaagacgg tgcggaagtc ggtcactttt gagagatcat cacttaagcg
     2581 gccataaccc acagcagttc cggctttgcc atcatcgcca acttcggaat aacttaacca
     2641 gatgcggcga gactgagcaa aatcaggcgc taaaaccacg tccagcaggc cgccctgccc
     2701 gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat aatccttttc ctgcttgcca
     2761 gtgacgcaac tcgccgccgc gcagagtgat taacataccg tgattatcgg gtaaaaaggc
     2821 cagtgcccag ggatggtcga gtttgtcttg cagtacttcg acatttaccg ttgcaggagc
     2881 cgcccagaga gcggaagaaa gacaaataag gggcacaagg aaaaaggatt gtcgatgcat
     2941 aacgcctccg gattgactct ggcttaaagc gtagtcagtg gaggagataa caaattcatt
     3001 tttacaaaaa cttaaacatg aagggggaga cgctttctcc cccttagttt tcaggccttc
     3061 tcaagcatgg cgtgcttctg caggctctgg atactcagcg ttaagctcat cagacaattt
     3121 tcaagcttat cggcgttgac ggtaataaca gtcgggcaat catggtgccc actcatcaaa
     3181 catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca ctcttcctga
     3241 tccagatgaa gattcaaccg cagcgattta tcgtgcagtt cgcgattcag tttaaaaaag
     3301 ttatctcgta gatgattgct ttcgctgacg gacatgtatc cttttgcctt cctcagttgc
     3361 acgtatgcag cgaggtcaac gcgtgcgtcg atcaaccggt tcagcagatc gattcgctgt
     3421 ctgtcaacga acatacttcg atcctcctct ttccagcctt gttgcaatta aatagtatgg
     3481 ctattttttg agcaaaatgc tgtccaggat caattcttca cgccgaatta acaaaattta
     3541 catttccttc gcctccgcca gcgaatgaga ttgcgataca aattttatcc agaactctgt
     3601 agaatccctg ccctgacctt tcctgtaact gaatattttt taagctatga gcaaagtaac
     3661 tccccagccg aaaatcggct ttgtttccct tggctgtccg aaaaaccttg tcgattcaga
     3721 gcgtattctc accgaactcc gcactgaagg ttatgacgtg gtaccgagct atgacgatgc
     3781 gaacatggtg atcgtcaaca cctgcggctt tattgacagc gcggtacaag aatcactgga
     3841 agccattggt gaagcgttga atgaaaacgg caaggtaatt gtgaccggtt gtctgggggc
     3901 aaaagaagat cagatccgcg aagtccaccc gaaagtgctg gaaatcaccg ggcctcatag
     3961 ctatgagcag gttctggagc acgttcatca ctacgtgcca aaaccgaaac acaacccatt
     4021 cctgagcctg gtgccagaac aaggtgtgaa actgacgccg cgtcattatg cctatctgaa
     4081 aatttcggaa ggctgtaatc accgctgcac cttctgcatt attccgtcta tgcgcggcga
     4141 cctgatgagc cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg tagatgcggg
     4201 cgttaaagag attctggtga tctcgcagga tacttccgcc tatggcgttg atgttaaaca
     4261 tcgtactggc ttccacaacg gcgagccggt aaaaaccagc atggtcagcc tgtgcgaaca
     4321 gttatcgaaa ctggggatct ggacacgtct gcactacgtt tacccttatc cgcatgtgga
     4381 cgacgtcatc ccactgatgg cagaaggcaa aatcctgccg tatctggaca ttccgttgca
     4441 gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag atcgccaact
     4501 ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg accctacgct caacctttat
     4561 tgtcggcttc cctggcgaga cggaagaaga tttccagatg ctactcgact tcctgaaaga
     4621 agcacgtctg gatcgcgttg gctgctttaa atacagcccg gttgaaggtg cagacgccaa
     4681 tgccctgcct gaccaggttc cggaagaagt gaaagaagaa cgctggaacc gtttcatgca
     4741 gctgcagcag cagatttccg ccgagcgcct gcaagagaaa gtgggccgtg aaattctggt
     4801 gattatcgac gaagtggacg aagaaggcgc gattggtcgc agcatggcag atgcaccgga
     4861 aatcgacggc gcggtttatc tcaatggtga aaccaacgtt aagccgggtg atatcctgcg
     4921 tgtgaaagtc gagcacgccg atgagtacga tttgtggggt agccgggttt aattaattca
     4981 ggccgggcaa aatcatttgt ccggcctgcg aataatgatt actgccagct aatctgtgcc
     5041 ctgccgtttt tcttactctt atacagccgc tcatccgcca gcattttcaa gcgttcgacg
     5101 gtagtacatt catttgaatg cgctacaccg atactggtgc ttaaattaat gcttcccacc
     5161 tggaaatgaa cgatttcttt tagtatcgat agcaactctt ccagcgtgcc gccagtataa
     5221 atcaccgcaa attcgtcacc tccgaagcgg tagactttac ctttatctac cgggatctgt
     5281 gatttaagga ttttcaccac tttcatcaac acttcatcgc ccagcaaatg accgaaggtg
     5341 tcgttaatgt ttttgaattt atcgacgtca aacagacaca aataaccgcc tttttccggc
     5401 agcgctttta aatcttcatc aaaggatttt ctgcccattg ctttggtcag gtcgtccagg
     5461 aacgccgagt tatacatact gtagagtttt ttgacatcgg cagccgaaaa gaaaagcccg
     5521 ccatccagta cgcgttcaaa atggttatta atgatggctt ctttctccac aataccacga
     5581 ttaatcactc tgcgtaccag atagaggcaa ctggcaccca atatcactgc caccaatgcg
     5641 gcaaagaaaa taatcaatgt acaaacccgc aaataaacat cttttttata cgcaaatacg
     5701 tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt gaagatggcg
     5761 tttttagcat tatattcatc accggtcacc ggtttaccgt tcttaaatag agagaacatc
     5821 aggttctcgc tgtccatcac cagcgatttt gacagtaaag aaaaacgctc cagaccaagc
     5881 aaaaatatca gactacgatt gtgagttacc ggatagaggt aatagacgaa ggttttaccg
     5941 tcaaaagaga taaaccctgt atttttgaca tgtcgatact tcacaacctg cataatatct
     6001 gcacaataat ttttctcgaa ctcctttacc tgaatacatt cgtcactgga aatcagataa
     6061 ggaccgacat cgtacaaata agagggggaa accgagtcgg cgtaagctgt gcgcaaacca
     6121 accaacagct gttcctccgt gggatttagc gtgttgataa gatcaattgc cacgctacgc
     6181 cgatattcag caatatttgc caccgcaaaa gcagtatcaa taacggactg tttcaggcta
     6241 tacacccgtt cctttaccat ttgagtgtag atcccgcagg caactgctga aatcatgata
     6301 aaaatcagca gactgactgt aagtacgaac ttattgattc tggacatacg taaactactc
     6361 ttttactaat tttccacttt tatcccaagc ggagaatggc atgggtttct ggtacagata
     6421 cccctgagcc tgaacacatc ctaatttaat taattcgcgc gcaacgtctt cgctctctac
     6481 gccttccgca atcaccggga tccggttata gcgtgccaga tcgaaaataa tgcgcacaat
     6541 ttttcccgca gtaccgaaaa tatcgccggt aaattgcgag tccaccttta tgctgtctgg
     6601 attaatttca taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat caatcgatat
     6661 ccgtacccct tgtttacgca gttgttcaat attgagataa aaatgttcat taattcgctc
     6721 gatcacggtt tcagtgatct caacacagag gctgttcgcc ttcaggccat tttctttcat
     6781 gatggtggta aaacgctcat gaaagtgcgg ttcgtttaac tgccagggcg aaacattaat
     6841 atgcagtaag aaatcatcct tcagcgacgc acgttgacta aaatgtctga attaacccat
     6901 tgccgtttta ataatctgat aacccagctc attgataaac ccgatatctt cagcaatggt
     6961 aatgaacttc agcggtgaaa tgatcccccg ctccgcagaa acccaacggc acaacgcctc
     7021 cgcgctcaga atggcgcggg tgttaatatc aacgatcggt tgatagacga ggaatagttc
     7081 gccaatttcc attgcatggc gaatattacg cgcaatccag atattctctt ctatttcgtt
     7141 gagtttcgcg tcatcacaga aaataaactc ggagaacata tggtcacgga tactggaaat
     7201 cgccagtttc agtttttctg cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat
     7261 ggcagactta tggatgcgca gattgcggta gccaaagctg ctgtcaaaat cgtttaccat
     7321 cgctatttta cgggtaaatg tctgtacatt ttcccgacac agaagtaaat agagatcggc
     7381 attgaatgtc acgatatcgc agcattgcgc atatttctcg gcaattcgct gacacaattt
     7441 cctgacgatt ttattgcgtt catcaacacc aaacaccgcc tccacggctt ccagaccaca
     7501 cagtttgatg atccccacag ccagatactg attattgttg gactcaataa tcgccatatt
     7561 tttaataaaa ccgctttcct gatcgtaaga gatcagtgag agcaatttat ttgaccgttc
     7621 aatttcggca tctttcattt ccgcgatcga atttttgatg ctgataatct ctttcgcatc
     7681 gccttcgcga attttcagag cctcgatctg atccgttgag tcgggcaaaa acgccagcgt
     7741 attaaccagc tcggtcattt cagaaaattg ccgacggaac acacgagtgg tcatgaaata
     7801 taccaccagc aacacgataa taatgaacgc aaacggtaga taaaaaagtt tatctgatgc
     7861 gttcaataaa ttaccgccag gataataaaa atagatttcg aacaatgcat tatttggcac
     7921 tgctgatgtc tttgcaatat actccgtatc gacagaatca ataatattat aattaccttc
     7981 gctattataa ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatgacc
     8041 ttgtttcaaa aatagaactt catcattagc gctgatttga tcaagatact cgttcagata
     8101 ccgccagttt aaatcgacat gaattttatc taccgctaac aaatcgtgat ctttgccgtt
     8161 gatacgtagc gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtatgccgg
     8221 attccagctg ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa
     8281 atcggcaaaa ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg
     8341 ataaacaacg attttatcat tcgccctccg cgaaaagaga taatatttat tatcatcaag
     8401 taccagtccc accgagtcta aaaatggcat catttccatt ttcagaatca cggcgcgttg
     8461 aatctcctct gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc
     8521 aatatcttta aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg
     8581 ttttaccgcg cgttccgata ccagttgata gttaatcatg aaaaaaagac caataaatga
     8641 cagtgctgcc aataaaaata aaattatcag ccttatcttt tcgtataaac tcagcatgtt
     8701 aatacattta acaaaataac tatctgaacg ctgtgccgct gcaaaaatag tcccttgctg
     8761 cggcttttta accaaattac ccgattctac aaaatcagca gaaagttaca acacttgctt
     8821 agggcaataa tttcctgtga ggtgattacc ctttcaagca atattcaaac gtaactatcc
     8881 tttaattttc ggatccagcg catcgcgtaa accatcgccc aacaaattga acgccagtac
     8941 ggtcagaaaa atagccaggg ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc
     9001 tcgtgcctca ttgagcattg ctccccactc tggtgtcggc ggctgcgcac cgaggccgag
     9061 aaatgacagg ctggcggcag agataatcga ggtgccaatg cgcatggtga aaaacaccac
     9121 gatagaagag acggtcccag gcaggatatg acgcaataaa atggtcatat cgctggcacc
     9181 aatactgcgt gctgactcaa taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac
     9241 caggcgggca aacgcgggga tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc
     9301 gcttcccaac accgcaacaa cagcgatcgc cagtaaaata cccgggaagg caaacagcac
     9361 atcgcaaatg cgcatgatca gccgatccca ccagccttca taatatccag ccaacaagcc
     9421 cagcaacgtc cctatcgcca caccgataaa cacggcaaac acgcccgccg ccagcgagat
     9481 ttgcgcacca accaggacac ggctgaaaat gtcgcgcccc agtgaatcga cgccaaacca
     9541 gtgctgcaaa gaaggtccgt tattcagatt gtcataatca aaataatttt cggcgtcata
     9601 gggagcgatc cagcgtgcaa aaatggccac cacaatcaat aaaataacga ataatgcggc
     9661 ggtcatcgcc atatgctgac ggcgaaatcg tcgccagaat tcatgccacg gtgtacgtac
     9721 ctggtcaggt ttgaccagtg gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag
     9781 tcgcatcctt acttgtacct gatagccggg ttaatggcgg cgtaaagcac atccaccact
     9841 aagttgataa gaataaattc cagcgagaaa agcagaattt ccgcctgaat caccgggtaa
     9901 tcgcgcattt ctacagagtc aacgagtaag cgtccaagtc cgggccagtt gaaaactttc
     9961 tccaccacga tagaaccacc gagcaaaaag ccaaactgta agcccatcat ggtcactacc
    10021 gggatcatcg cgttgcgtag cccgtgtttg aggacaaccc aggtttcgct cacccctttc
    10081 gccctcgcgg tacgcatata atcttcgctt aaaacgtcga caaatgacgc acgggtaaag
    10141 cgcgccatca cggcggccac tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc
    10201 caactgtctg ctcccacagt aggcagccag cccagttcaa cggagaatac ctgaattaaa
    10261 aacatcccca gcgcaaatgc cggaaacgag atccccgaca ccgcaatggt catactcaat
    10321 cgatccggcc aacgattacg ccagacggcg gcgataattc ccgccgccat accaaatatc
    10381 accgcccaga ccatactggt tatggtcagc catagcgttg gcataaagcg gctggcaatc
    10441 tcatcggcaa ccggacgacg cgacaccatc gacaggccaa aatccccctg cacagcattg
    10501 ctgatatagt gccagaactg gtgatacagc ggctgatcca accccagctg ctgacgcacc
    10561 agttctataa cctgcgcatc agcttcgggc ccggcaatca atcgcgccgg atcgccgggc
    10621 agcatgtgga caaataaaaa caccagcacc gagacgataa acagcgtcgg aatcaacccc
    10681 agtaatcgtt tgataacgta ataaagcatt ccactccctg cgttgcttat tgcaaatccg
    10741 cgtcttcaaa gctgaagccg gtatctggca tgatccaaaa accggtcagg tttttactgt
    10801 gtgccgacac cagtttttct accaccagcg ggatccacgg cgattcttgc cagatgatat
    10861 cctgcgccgc cttatataag cgggtctttt ccgccggatc attcgttttc agtgcctgag
    10921 ccaggaagtc atccacctgt ttattgctgt aaaacgcggt attaaacagc gtcggtggcc
    10981 agttttgcga ggcaaacagc ggcgatagtg cccagtcggc ttcgccggtt gaagccgacc
    11041 agccagtgta gaacatccgc acgccactct ctttttgccc tttaccttca acttctgccg
    11101 cccgctgtcc ggcatccatc gcggtcacct gggctttaat cccgacctgc gctaactgct
    11161 gctgggtaaa ttgcagcact ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg
    11221 tactgaaacc gttgggatat cccgcctctt tcagtaattc gcgcgctttc actggatcgt
    11281 aaggccacgg tttataactt tgcgcgtagg cgatacttgg cggtaccaca ccagtagctg
    11341 gagttgcata gcccgcaaag gcaactttca ccagcgccgg acggttaatg gcgtaattca
    11401 gcgcctcacg gaccttcggg ttatcgaacg gcttttgcgt cacgttcata ctgatataac
    11461 gctgcataat tgacggactg gccatcaact cgatattttt gtttttctcc agcagtgtgg
    11521 cctgctcgta gggaatgggg aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc
    11581 gggtgttgtt atccgccacc ggacgccagg ttatgctgtc cagtttgggc aatcctggct
    11641 gccagtaacc cgcgaatttt ttcaccttca caaaatcggt ctgattccag gtatccagtt
    11701 catacggtcc ggttcccacc ggatgaaaac caatctcctt gccatatttt tccagcgctg
    11761 tcggtgaaat catcgcggtc gccggatggg caagaatatt aataaacgct gagaacggct
    11821 gtttgagggt aatctttacc gttgtcggat cgatcgcctc cgttttggca atattcttat
    11881 acaggttata gcgtttaaga tgattcgccg gatcgctggc ccggtccaga ttcgctttta
    11941 ccgccactgc gttgaaatcg gtgccatcct ggaatttaat tccttcccgc aatttcacgg
    12001 tgtaagtaat gccgtcatcg gaaacggtat aactctccgc cagcacgttt ttcagtttca
    12061 tctctttatc cagaccgaac agcccctggt aaaacgattt cgctacggcc tgagataacg
    12121 tgtcattcgc gtcatacgga tcgagcgtgg tgaaattcga tcctaccgcc accaccacat
    12181 ctttggcagc gaatgcacaa gatgccatca acgctgtcgc aatgcccagc gccactaacc
    12241 cactacggtg tacagctctt gccattttat tctccgcctg aatgttatct acgcatgaat
    12301 gcgtattctg attgtggttg tgcgacgtaa tgccccggcc cgacgcattg caacgagacg
    12361 gctgccacct cttcgccacg cagatgaata ttgctgggaa gatcgtccga cagcagtaca
    12421 cgctgcggtc gttgtcggga cggttcagcg accggaactg ccgccagtaa tttacgcgta
    12481 taaggatgct gcgggttttc gaagaccgcg caccgtgaac caatttcaac aatttgcccg
    12541 agatacatca ccgccacacg atgactaatc cgctctacca cagccatatc gtgggagata
    12601 aacagatacg caatgccgaa atcacgctgg agatcgagca acaagttgat aatctgcccg
    12661 cgaatagaaa catccagcgc cgaaacggct tcgtcggcaa tgatcacttt tggattcaat
    12721 gccaacgcgc gagcaatgca gatgcgctgg cgctgaccgc cggaaaactc atgcgggtaa
    12781 cgccaggcat gttcaggtaa caggcccacg cgctccagca accacgcaac gcgtgcagcc
    12841 gcgtctttac ctggcaataa accgtgtaca cgcagcggtt cgataatcga atcaccgatg
    12901 gtctgacgtg ggtccagcga agcgtaaggg tcctgaaaaa taaactgaat atcccggcgc
    12961 aatgcctgaa gtttgccagg tgacaaggta tcgattcgct gaccgttaaa gataatttcg
    13021 ccgccctgcg attcgaccag gcgcagcaac gcccgcccgg tagtggattt accgctgcca
    13081 gactcgccca ccagcgatag cgtttcgcca ggccagagat caaaactgac tttctcaacg
    13141 gcatgcactt cccgcgttac gcgattcaac aaaccgctgc gcaaagggaa acgggtgaca
    13201 agattacgca cccgtaaaac aggttcgcca tccaccaccg ttttctgctc gatggggggt
    13261 tcctgtttcg ctggatgttc aagcgatatc aatgggaaac gtcggggata atctaaccct
    13321 ttcatcgcac caagttgcgg aacagcagct aacagcgcac gggtgtaagg atgttgcggt
    13381 gcatgaaaaa tctgttcgac ggtacccgtt tccaccgcct cgccctgata catcaccagt
    13441 acccgatcgg caatctctgc caccacgccc atatcgtgag tgataaagat aacgcccatc
    13501 gacatctctt tttgcaatac tttgattaat tgcaggatct gcgcctgaat agtgacatcc
    13561 agcgcggtgg ttggctcatc ggcaatcagc accgccgggc ggcatgacag cgccatcgca
    13621 atcatcactc gctggcgcat cccgccagag agttgatgcg aataacgtga aagaatggtt
    13681 tgtgcctccg gaatgcgtac ctgatccagc atccgcttcg cctcgaccat cgcttcttca
    13741 cgactggcgt tctgatgcag acgaattgat tcggcaatct gttcacccac agtaaatact
    13801 gggttcagcg atgtcatcgg ctcctgaaat atcatcgcca tatccgcacc gcgcacatgg
    13861 cgcatttgtg cagcgctctg ctcgctaagt tcaatcacat cacgactgcg ccgccgcaac
    13921 agcattttat cgcactgtac taaaccgccc gcctgttcca acagacgcat caatgccagc
    13981 gcagtcactg acttaccgga gccggattcg cctacaattg ccagcgtctc accgcgttgc
    14041 agactaaaag agagattgcg gaccgcagct attttctgct ggtcctgcat aaaggcaata
    14101 ttaagatttt caaccgccag cacattaccg gcatcaagtt catcactgtg tggcaacggt
    14161 gtcccctttt tcacggtaga taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg
    14221 atacattcct tcggtgttaa acggtagcgc gacattccct tcatggtcga tagcgattaa
    14281 gccaccgcta ccgccaagcg cagggagttt ttccattact acccgctcgc aggcttccgc
    14341 gagacttaat ccgccgtaat ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat
    14401 gaagacttcg cctgtgccgg tacaagaaac cgccacactg gcgttattgg cgtagcatcc
    14461 ggcacccact aagggactat cgccaactcg tccgggtaat ttattggtca ttccgcctgt
    14521 ggacgtggct gccgccagat tgccgtctaa atccaacgcc acggccccca cggtgcccat
    14581 tttctgtttt tcatccagtg gcgcaccgct atggtcgagg actgttgccc cttcctcgcg
    14641 cgctgccagt agttgttcat aacgcaaagg cgtggagaaa atctccggtg agacgcgctc
    14701 catgccatga gcaaacgcaa aattttctgc cccttcgcca atcatcatca catgcgggct
    14761 ttgctccatc accagccggg cggcaagaac cggattacgc agatgactaa cgcccgccac
    14821 cgcaccggct ttcagggtgt taccatccat cacacaggcg tccagttcat gggtttcatc
    14881 acgcgtaaag acagcgccaa ttccggcgtt aaacagtgga cactcttcca gcagacgcac
    14941 cgcttccgtc accacatcca gcgcactttc gccggcttcc agcattttct gcccggtttc
    15001 aacaatggca gacaacgcct cgatgtagcg taattcctgt tgcagactca tctgcgcgcg
    15061 gctaattgca cctgcgccac catgaattgc aatgactgct ttgcccatag tattcgtcca
    15121 ttaagggtgg ctcgccactt ttgctataaa tatcagaatt tttatgaatt actcattcaa
    15181 tttttgaata tagaaagatg gatatggcat gtaaaggcag gagccaacgc tacgcccgag
    15241 gtggcataca gtctatggcg tttttctgac ataataggca aattcgattt tgcctccgca
    15301 ggagtgtttt catggaattt accaccggat tgatgtcgct cgacaccgcg cttaatgaga
    15361 tgctttctcg cgttacccca ctgaccaccc aggaaacgct gccactggtt cagtgttttg
    15421 gtcgtattct ggcgagcgat gtcgtttcgc cactggatgt cccggggttt gataactccg
    15481 ctatggacgg ctacgcggtg cgtttagccg atattgcctc cgggcaaccg ctgcccgttg
    15541 ccggtaaatc ctttgccggt cagccatacc atggtgaatg gcctgcgggt acctgcattc
    15601 gtattatgac cggtgcgccg gtgccggaag gctgcgaagc ggtggtgatg caggagcaga
    15661 ctgaacaaac ggacaatggc gtgcgtttta ctgctgaagt gcgtagcggg caaaatattc
    15721 gccgtcgcgg tgaagatatc tctgcaggtg cggttgtttt cccggcggga actcgcctga
    15781 ctaccgcaga gctgccagta attgcttcac tggggattgc cgaagttccg gtgattcgta
    15841 aagtgcgtgt agcgcttttt tctaccggtg atgaactcca gttgcccggt cagccgctgg
    15901 gcgacggcca aatctacgac accaaccgtc tcgccgtaca cctgatgtta gaacagctgg
    15961 gttgcgaggt aatcaactta gggattatcc gcgacgatcc acatgccctg cgcgccgcat
    16021 ttattgaagc cgacagccag gcggatgtag tgatcagttc cggcggtgtt tcagtgggtg
    16081 aagcggatta caccaaaatg attcttgaag agctggggga gatcgccttc tggaagctgg
    16141 cgattaaacc aggtaaaccg ttcgcgttcg gtaaactcag caatagctgg ttctgcggcc
    16201 tgccgggcaa cccggtttca gcgacgctga ccttctatca actggtacag cctttgctgg
    16261 caaaactaag cggcaacacc gccagcggcc tgcccgcgcg ccagcgcgta cgcacagcgt
    16321 cccgcctgaa aaaaacgcca ggacgtcttg atttccagcg cggcgtgctg caacgcaacg
    16381 ccgacggtga acttgaagtg acgaccaccg gacatcaggg ttcacatata tttagctcct
    16441 ttagtctcgg caactgcttt atcgtgctgg aacgcgatcg cggcaatgtg gacgtgcgcg
    16501 aatgggtgga agtagaaccg tttaacgcgt tgttcggagg cctgtaatgg cggaactcag
    16561 cgatcaggag atgctgcgct acaaccggca aatcattctg cgcggttttg attttgacgg
    16621 tcaggaggcg ctaaaagatt ctcgtgtgct ggtagtcggc ctcggtggac tcggctgcgc
    16681 agcttcgcag tatctggcaa gcgccggtgt cggcaacctg acgctgctcg acttcgacac
    16741 ggtttcgctc tcgaatctgc aacgccagac actgcacagt gatgccacgg tcgggcaacc
    16801 gaaggtggaa tccgcccgtg acgccctgac gcggatcaac ccacatatcg cgattacgcc
    16861 agtcaatgca ctgctagatg acgcagaact tgcagcattg attgctgaac acgatctggt
    16921 gctcgactgt acggataacg ttgcggtacg taatcaactg aacgcaggct gttttgccgc
    16981 gaaggtgccg ctggtttccg gcgcggcaat tcgtatggaa gggcaaatca cggtcttcac
    17041 ttatcaggac ggcgaaccgt gctatcgctg ccttagccgt ttgtttggcg agaacgcgtt
    17101 aacctgcgta gaagcaggcg tgatggcacc gttgataggc gtaattggtt cgttgcaggc
    17161 aatggaagcg atcaaactgc tggcaggtta tggcaaaccg gccagcggga aaatcgtcat
    17221 gtacgatgcg atgacctgtc agtttcgcga aatgaaactg atgcgcaatc cggggtgtga
    17281 ggtgtgcggg cagtaattgc cacctccaag cctgataaga cgcgacaagc gtcgcatcag
    17341 gcatttgctc cagacgccag atgcggcgtg aacgccttat ctggcctacg gttcgggcac
    17401 agatttccaa gcgcggcagg catcggtgca ctccgccacg tcatcagcaa tttatgagga
    17461 tgcagaacgt ccggttaaat cgacgttctg ccaaacgctc cctgccagtc ctgctcaaac
    17521 ttcgtcacag cggcttccac cgccggatag ctaatcatct gttgtgccac atccagtggc
    17581 agagtaattg attcacatcc ggccagtaag cagtccagcg cctgacgcgg ggttttgaaa
    17641 ctcgctgcca gcactttcgc ctgcggcgca tgcattttca ataactggtg taagtcggtc
    17701 acagtctgaa tgccgctacc gccctgagca tcaatacgat taacgtaagg cgcaacatat
    17761 tccgcacctg ccagcgccga cagcagccct tgtgccgcgc catataccgc ggttcccagc
    17821 gtcggaatcc cttccgcttt taacatctta atcgctgcca gcccctcggc tgtcaccgga
    17881 actttcacca cgatatccgc aataatagaa cgcagcttaa gcgcgtcatt caccatccct
    17941 tcagcagtgg tagccattac ctgggcaaac agacgcccct gaccgcccat cgcttcatga
    18001 agttgcggaa gcacaacatc cagcggtttt ttacccgcgg cgataatgct tgggttagtg
    18061 gtcacacccg ccagcggaaa aatacgtgac agcgccttca ccgcaacaac gtctgaagta
    18121 tccagataca gttccataac catcctcaaa atgtactttt tgtctgaatg tagactatca
    18181 cgggaaaact ctctgaacag ttgacgcaca tcaatctaac tttcattcga aagtatttta
    18241 atgtttgaac gaaacagaga ggcaactatg attttcaata ttcagcgcta ctcgacccat
    18301 gatggccccg gtatccgcac ggtcgtattt cttaaaggct gttcgctggg ctgccgctgg
    18361 tgtcagaacc cggaaagccg cgcccgtacg caggatctgc tgtatgacgc acgactgtgt
    18421 ctggaaggct gcgagctgtg cgctaaggcc gcgccggaag tgattgagcg cgcgctgaat
    18481 ggtttgctta ttcatcggga aaagttaacc ccggagcatc tgacggcgtt aaccgactgc
    18541 tgtccgacac aggcgttaac cgtgtgtggt gaagtgaaaa gcgttgagga gatcatggcg
    18601 accgttctgc gcgataaacc gttttacgat cgcagcggcg gcggcttaac gctttcgggt
    18661 ggtgaaccct tcatgcagcc ggaaatggcg acggcgctgc tgcaagcctg ccacgaggca
    18721 ggcattcata ctgcggtaga aacctgtctg catgtgccgt ggaaatatat cgccccttct
    18781 ctgccctata tcgatctgtt tcttgccgat ttaaaacacg ttgccgacgc gccgtttaaa
    18841 cagtggaccg acggtaacgc cgccagggtg ctggataacc tgaaaaaact cgccgcagcg
    18901 ggcaaaaaaa tcattatccg cgtgccgctg attcagggct ttaatgccga cgaaacctct
    18961 gtaaaagcca ttaccgattt tgccgccgac gagctgcacg ttggcgaaat tcattttctg
    19021 ccctaccaca cgctgggcat caacaaatat cacttactta atctgcccta tgacgccccg
    19081 gaaaaaccgc ttgatgcgcc agagctgctc gattttgccc agcagtatgc ctgccagaaa
    19141 gggttaaccg cgaccttacg aggataacaa ccatgaccac actgaaactg gacacgctca
    19201 gcgaccgcat taaagcgcac aaaaatgcgc tggtgcatat tgtgaagccg ccagtctgta
    19261 ccgagcgcgc gcagcactat accgagatgt atcaacaaca tctcgataag ccgatcccgg
    19321 tacgtcgcgc gctggcactg gcgcatcacc tggcgaatcg caccatctgg atcaaacacg
    19381 atgagttgat cattggcaac caggcaagcg aagttcgcgc cgcgccgatc ttcccggaat
    19441 atactgtctc gtggatcgaa aaagagattg atgatctggc agatcgtccc ggtgctggct
    19501 ttgcggtgag cgaagagaac aaacgtgttc tgcatgaagt gtgcccgtgg tggcgcggtc
    19561 agacggtaca ggatcgctgc tatggcatgt ttaccgatga gcaaaaaggt ctgctggcga
    19621 ccggcatcat taaagcggaa ggcaatatga cctccggcga tgcgcacctg gcggtcaatt
    19681 tcccgctgct gctggaaaaa gggcttgatg gtctgcgtga gaaagtagcg gaacgtcgct
    19741 cgcgcatcaa tctgacggtg ctggaagatt tgcacggcga acagttcctg aaagcgattg
    19801 atatcatgct ggtggcagtc agtgaacaca ttgaacgttt cgctgccctg gcgcgtgcaa
    19861 tggccgcgac cgaaacccgc gaaagccgtc gcgatgaact gctggcgatg gcagaaaact
    19921 gcgatcttat cgcccaccag ccgccgcaga ctttctggca ggcgctgcaa ctgtgttact
    19981 tcatccagtt gattttgcag attgaatcta acggtcactc ggtgtcgttt ggtcgtatgg
    20041 atcagtatct ctacccgtac tatcgccgcg acgttgaact gaaccagacg ctggatcgcg
    20101 aacacgccat cgaattgctg catagctgct ggttgaaatt gctggaagtg aacaaaatcc
    20161 gctccggctc ccactcaaaa gcctctgcgg gaagtccgct gtatcagaac gtcaccattg
    20221 gcggacaaaa tctggttgat ggtcaaccaa tggacgcggt gaatccactc tcttacgcga
    20281 tcctcgaatc ctgcggtcgc ctgcgttcca ctcagcctaa cctcagcgtg cgttaccatg
    20341 caggaatgag taacgatttc ctcgacgcct gcgtacatgt gatccgctgc ggcttcggga
    20401 tgccggcatt caacaacgac gaaatcgtga tcccggaatt tattaaactc ggtattgaac
    20461 cgcaggacgc ttatgactac gcagcgattg gttgtataga aaccgccgtc ggtggcaaat
    20521 ggggctatcg ctgtaccggc atgagcttta tcaacttcgc ccgcgtgatg ctggcggcgc
    20581 tggaaggcgg gcgtgatgcc accagcggca aagtgttcct gccacaagaa aaagcgttgt
    20641 cggcaggtaa cttcaacaac ttcgatgaag tgatggacgc gtgggatacg caaatccgtt
    20701 actacacgcg taaatcaatc gaaatcgaat atgtcgtcga caccatgctg gaagagaacg
    20761 tgcacgatat tctctgctcg gcgctggtgg atgactgtat tgagcgagcg aaaagtatca
    20821 agcaaggcgg cgcgaaatat gactgggttt ctggcctgca ggtcggtatt gccaacctcg
    20881 gcaacagcct ggcggcagtg aagaaactgg tgtttgaaca gggcgcgatt ggtcagcaac
    20941 agctggctgc cgcactggcg gatgacttcg atggcctgac tcatgagcaa ctgcgtcagc
    21001 ggctgattaa cggtgcgccg aagtacggca acgacgatga taccgtcgat acgctgctgg
    21061 ctcgcgctta tcagacctat atcgacgaac tgaaacagta ccataaccca cgctacggtc
    21121 gtggtccggt tggcggcaac tattacgcgg gtacgtcgtc tatctccgct aacgtaccgt
    21181 ttggcgcgca aactatggca acgccggacg ggcgtaaagc ccacaccccg ctggcagaag
    21241 gcgctagtcc ggcctctggt actgaccatc tcggccctac tgcggtcatt ggctcagtgg
    21301 gtaaactgcc tacggcagcg attctcggcg gcgtgttgct caaccagaaa ctgaatccgg
    21361 caacgctgga gaacgaatct gacaagcaga aactgatgat cctgctgcgt accttctttg
    21421 aagtgcataa aggctggcat attcagtaca acatcgtttc ccgcgaaacg ctgctggagg
    21481 cgaaaaaaca tccggatcag taccgcgatc tggtagtgcg tgtcgcgggt tattccgcct
    21541 tcttcaccgc gctctctcca gacgctcagg acgatatcat cgcccgtact gaacatatgc
    21601 tgtaatctct tcggcccggc tgcggcgtcg ccgggctaaa tcattcacat catcaatttc
    21661 atccttactt tcattcgaaa tataatttgt gctctgcgtc acattgttat tacactgtta
    21721 cggtttcatt ttcgagccag gagcacacct atgagcgtaa aagttatcgt cacagatatg
    21781 gacggtactt ttcttaacga cgccaaaacg tacaaccaac cacgttttat ggcgcaatat
    21841 caggaactga aaaagcgcgg cattaagttc gttgtcgcca gcggtaatca gtattaccag
    21901 cttatttcgt tctttcctga gctaaaggat gagatctctt ttgtcgcgga aaacggcgca
    21961 ctggtttacg aacatggcaa gcaactgttc cacggcgaac tgacccgaca tgaatcgcgg
    22021 attgttattg gcgagttgct aaaagataag caactcaatt ttgtcgcctg cggtctgcaa
    22081 agtgcatatg tcagcgaaaa tgcccccgaa gcatttgtcg cactgatggc aaaacactac
    22141 catcgcctga aacctgtaaa agattatcag gagattgacg acgtactgtt caagttttcg
    22201 ctcaacctgc cggatgaaca aatcccgtta gtgatcgaca aactacacat agcgctggat
    22261 ggcattatga aacccgtcac cagtggtttt ggctttatcg acctgattat tcccggtcta
    22321 cataaagcaa acggtatttc gcggttactg aaacgctggg atctgtcacc gcaaaatgtg
    22381 gtagcgattg gcgacagcgg taacgatgcg gagatgctga aaatggcgcg ttattccttt
    22441 gcgatgggca atgctgcgga aaacattaaa caaatcgccc gttacgctac cgatgataat
    22501 aatcatgaag gcgcgctgaa tgtgattcag gctgtgctgg ataacacatc cccttttaac
    22561 agctgacctc tctaccggag ccatgcgctc cggttttccc ttctttttac atcctgtaca
    22621 tcacatcaaa tcattaaact tgcattaact tatttataac ttgtattgtc acatgcaaat
    22681 tagcatagtt tcgaatgaaa gttaatgcaa ggtggttatg gcttctactt ttaccagcga
    22741 cacattgcct gccgatcaca aagcagctat ccgtcagatg aagcacgcgc tgcgggcgca
    22801 gcttggcgat gtccagcaga tctttaatca gctaagcgat gacattgcca cgcgagtggc
    22861 tgaaatcaac gcactcaaag cacagggcga tgccgtctgg ccggtgctgt cttatgccga
    22921 tatcaaagca ggtcatgtta ctgcagagca gcgcgaacag attaaacgtc gcggttgtgc
    22981 ggtgataaaa ggccatttcc cccgcgaaca agcgctaggc tgggatcagt cgatgctgga
    23041 ctatctgaac cgcaaccgct ttgacgaggt ctacaaaggc cccggcgata atttcttcgg
    23101 gacgctcagc gcttcacgtc ccgagattta ccccatctac tggtcgcagg cgcaaatgca
    23161 ggcccgccag agtgaagaaa tggcgaatgc gcagtcgttt ctcaatcgtc tgtggacatt
    23221 tgaaagtgat ggaaagcaat ggtttaaccc ggatgtgagc gtcatctacc ctgaccgtat
    23281 ccgccgcctt ccgcccggaa cgacctccaa aggtcttgga gcgcataccg actccggggc
    23341 gctggaacgc tggctgcttc cagcgtatca gcacgttttc gccaacgtct ttaatggcaa
    23401 tctggcgaaa tacgatccct ggcatgcggc acatcgtacg gaagttgaag agtacacggt
    23461 ggacaacacc accaaatgtt ccgtgtttcg gacattccag ggctggactg cgctctcgga
    23521 tatgctgcct ggtcaggggt tgctgcacgt cgtgcccatt cctgaagcca tggcgtacgt
    23581 actgttacgt ccgctgcttg atgatgtgcc ggaggatgaa ctgtgcggcg tagcgcccgg
    23641 aagagtgttg ccggtatcag agcaatggca tccactgtta attgaggcgt taaccagcat
    23701 tccaaaactc gaggccggag actccgtctg gtggcactgc gacgtcatcc attccgttgc
    23761 ccccgttgaa aatcaacagg gttggggcaa cgtgatgtac attcctgcgg caccgatgtg
    23821 cgagaaaaat cttgcctacg cgcacaaggt gaaggccgca ctggaaaaag gcgcatcgcc
    23881 gggcgacttc ccgcgcgagg actatgaaac aaactgggaa ggacgcttta cgcttgccga
    23941 cctcaacatt cacggtaagc gagcgttggg catggatgtt tgagagttaa tggaagtgaa
    24001 ttgatgtggg attaagaaaa tcgattcgac gttactcaga ttgctgacaa gtgcgctttc
    24061 ttaatgcctg atgcggcgtt aacgccttat ccggcctaca aagtcctgca gattcaatat
    24121 attacaggta ctgcgtaggc ctgataagcg tagcgcatca ggctattttg catttgtcat
    24181 cagccgtcgt caccccgaca gtatcggggt gacggataat cacttactcg atccctttac
    24241 tacgcaggta atcttcgtaa ttaccgctaa agtcgatcac gcgttccggg gtgatttcca
    24301 gaatgcgggt cgccagggag cttacgaact cacggtcgtg tgaaacaaag atcagcgtgc
    24361 cctgatacag ttccagcgcc atgttcagtg actcgatgga ttccatatcc aggtggttgg
    24421 tcggttcgtc catgatcaga atgttcggct tctgcatcat taacttacca aacagcatcc
    24481 gccctttttc cccaccggaa agcactttag ctggcttttt gatgtcgtcc tggctgaaca
    24541 gcaaacgacc gagaatgctg cgtaccgcct gctcgtcatc gccttcctgc ttccactgac
    24601 tcatccattc gaacacggtc agatcatttt caaactcata ttcgtggtcc tgggcgtagt
    24661 aaccaatgcg cgcgttttca gaccatttta cggtgccgct gtccggttgc agatcgccca
    24721 ccagcgtttt cagcagcgtt gatttaccga cgccgttggt acccagtacc gcaagttttt
    24781 caccgacctc cagcagcagg ttgagatttt taaacagtgg accgttatca aaccctttgg
    24841 tcagaccttc cacttccagc gcgttacgga acagtttctt atcctgttca aaacggataa
    24901 acgggttctg acggctggag gctttcacct cttccagttt gattttatca atctggcgcg
    24961 cgcgcgaagt tgcctggcga gatttcgagg cgttggcgct aaagcggcta acgaaagatt
    25021 gcaactcagc aatctgcgct ttcttcttgg cgttatcggc cagcagacgt tcacgcgctt
    25081 gggtcgccgc cgtcatgtac tcatcgtagt tacccggata aacgcgcagc tcgccgtaat
    25141 ccagatccgc catgtgggta cagaccatgt taaggaagtg acggtcgtgc gagatgatga
    25201 tcatggtgct gtcacgctcg ttcagcacct gttccagcca gcgaatggtg tcgatgtcga
    25261 ggttgttggt cggttcgtcg agcaggagaa tatccggctc agcaaacagc gcctgcgcca
    25321 gaagcacacg cagcttccag ccaggagcaa cttcactcat cgggccgtag tgctgttcca
    25381 ctggaattcc cacgccaagc aacagttcac cggcgcgagc ttccgcagag taaccgtcca
    25441 tttcgccgta tttcacttcc agatcggcca ctttatagcc gtcttcttca ctcatttccg
    25501 gcaaagcata gatgcggtcg cgctcctgct tcacttccca caactcttta tgccccatga
    25561 tcaccgtatc cagcacagtg aactcttcaa aggcaaactg atcctgacgc agcttaccaa
    25621 tgcgctcgtt gggatcgagg gaaacgttac ccagcgtcgg ctcaaggtcg ccgccgagaa
    25681 tcttcataaa ggtggattta ccactaccgt tcgcgccaat caggccgtaa cggttgccgc
    25741 cgccgaattt gacggaaatg ttttcaaaca acggcttact gccgaactgc atggtgacgt
    25801 tactggaaac taacacaggc gtatcctgaa aagagatatg acaaaccgcg cattatgcca
    25861 taagtctgaa agaagatcct gctcctcgct taaggtctac actcaagcca aaggcaaaaa
    25921 atgtgatttc gtacacatct gatttcactg tgagctggaa tgaacttata atgcgcttcc
    25981 aatactctaa tattctcaac ccaatggcct gccaggcaca aaatctcgct taacatgaat
    26041 atgaaattga aaacattatt cgcagcggcc ttcgctgttg tcggcttttg cagtaccgcc
    26101 tctgcggtaa cttatcctct gccaaccgac gggagtcgcc tggttggtca gaatcaggtg
    26161 atcaccattc ctgaaggtaa cactcagccg ctggagtatt ttgccgcgga gtaccagatg
    26221 gggctttcca atatgatgga agcgaacccg ggtgtggata ccttcctgcc gaaaggcggt
    26281 actgtactga acattccgca gcagctgatc ctgccggata ccgttcatga aggcatcgtc
    26341 attaacagtg cagagatgcg tctgtattac tatccgaaag ggaccaacac cgttatcgtg
    26401 ctgccgatcg gcattggtca gttaggcaaa gatacgccta tcaactggac caccaaagtt
    26461 gagcataaga aagcaggccc gacctggacg ccgaccgcca aaatgcacgc agagtaccgc
    26521 gctgcgggcg aaccgctacc ggctgtcgtt ccggcaggtc cggataaccc gatgggtctg
    26581 tatgcactct acatcggtcg cctgtatgct atccatggca ccaacgccaa cttcggtatc
    26641 ggcctgcgtg taagtcatgg ttgtgtgcgt ctgcgtaacg aagacatcaa attcctgttc
    26701 gagaaagtac cggtcggtac ccgcgtacag tttattgatg agccggtaaa agcgaccacc
    26761 gagccagacg gcagccgtta tattgaagtc cataacccgc tgtctaccac cgaagcccag
    26821 tttgaaggtc aggaaatcgt gccaattacc ctgacgaaga gcgtgcagac agtgaccggt
    26881 cagccagatg ttgaccaggt tgttcttgat gaagcgatca aaaatcgttc cgggatgccg
    26941 gttcgcctga attaatcgtc aaaacgtaaa acaaaggcgg actcataatc cgcctttttt
    27001 atttgccaga ccttaccagg ccgggatcat aactaacaaa acgtatccca ccagcgccgc
    27061 ccacaacagc atcggtattg aatagagatg gaaacgccac cagatgcggc gatcgttcgc
    27121 catacgtagc gcaatcaaat ttgccagcga tcctggtaat aaaccaaagc cccccacgtt
    27181 taccgcccat gccagtaata aagacggagg tacataattg agcagcaaaa tggtactcgg
    27241 cacgttactg atcacctgcg ataaaccgac tgccgttaac cataacccgg gttcagataa
    27301 atgactcacg ttacccaaca cgccttgcaa cgctggaagc tgggtcagta aatggacgtc
    27361 gataaacatc gccataaaca ccagcagcaa cgtccagtcc acactcaaca ccacgcgacg
    27421 cgccagcagc acaaagcccg ccgccacaat caccagtccc cacagctctt gtttgaactc
    27481 cagcgccgtc agaaagacga tatacagccc cagacaattc cacaccagcc gcggtttcca
    27541 ctccggtgtt tgcacccccg tatggtattg cagcgccttt ccagggaaac aacaccagca
    27601 caacagcagg agtgtcagca tcattgcgcc agccatcggt gccatttggg caataaatcc
    27661 ggcaaacgaa agaccagaac gtccccagat aagaatattt tgcgggttgc caattggcgt
    27721 cagtagcgaa ccagcgttga ctgccagcgc ctcaaaaata atcagccgat taaccgggat
    27781 ctcacacagt ctttttagcg tgatagtcag cggaacaaca ataaacagcg cgacatcgtt
    27841 ggtcagaaag gtagaaagca gcgccgccgc cagcaccata aacatcgcca gccgacgctc
    27901 cgtagcaaag cggcgcacca ttttgcgccc cagcacatca aaataaccgc ttaactccac
    27961 acctttggtc agcagcatca ggccgcttaa ggtgatgatg gtgtgccagt cgatagcagc
    28021 aggccaggat ttcggtgcaa agggcacgaa aaagcttaat ccgataccaa caagaattaa
    28081 taactgaaaa aaacgatcgc cttgcagcgt gcgtaaaaaa ggcaggctca tttggcaccg
    28141 tgtttctggg taaacaaacg aaaagcgtcg agcgtctctt cactaacatg gtgctccatg
    28201 ccttccgcgt cgcgacgggc gatttccgga ctgacgccca acaccaacaa gaaattttcg
    28261 actatctgat gtcgctcgcg gctttcctgc gccagcttct ctccttctgc cgttaaaaac
    28321 acgccacgcc aggggatcat ttcaatcagc cccatggttg ccagccgctt aagcatttta
    28381 gccaccgtcg gttgcgaaac tcccagacga gcagccatgt ccacctgacg agcttccccc
    28441 acttccctga tcaagtcaga aatcagctca acgtaatcat caataagctc gcgccgatgc
    28501 gcctctctga cctggcggaa cccttcaacg tgctcttcca cgttcactaa ctgcgtcact
    28561 ttttttgcta ttggcgtacc tgcgcgacga ctcattgtgc ttcctcatta tggtgacgca
    28621 tacatagcgt tctcatttcg tgcgcacatt gtaaaccaga gttgcgaagg tacaaaaaat
    28681 taacgtttta gcaatagcta tataatatag cctgtgctat atctgtatgt aatgcaatca
    28741 tccctcaagg atcgacggga ttagcaagtc aggaggtctt atgaatgagt tcaagaggtg
    28801 tatgcgcgtg tttagtcatt ctccctttaa agtacggtta atgctgctct ctatgttgtg
    28861 cgatatggtc aacaacaaac cgcagcaaga taaaccttca gataaatagc ggcgtcgcgg
    28921 tacgccgctt cactcctgct tttatgcagg cataacgcgt tttggtctga aaaaaaccac
    28981 tttttgtcgg atttgcaatc cccttcgcaa aagatttgtt cgtcagtagt tgacctgaac
    29041 ggcggctcgc tctatcttct tgcagccctg cgtatattgc ggctcgcgga tgcggacccc
    29101 tttcccctct tcacgcactc ttgcaggtat tgacccttga cgccagggta agcacatggc
    29161 gtttgttacg atagtggcat atgaatttaa ccctcaaaga atcgcttgtt acccgtagcc
    29221 gggtatttag cccgtggact gcgttctact ttttacagtc gctattaatt aacctcggct
    29281 taggttaccc cttcagtttg ctctacaccg ctgcgtttac ggctattttg cttttgctat
    29341 ggcgaacatt gcctcgcgta caaaaagttc tggtcggtgt cagttcgctg gtggcggctt
    29401 gttatttccc ttttgctcag gcctacggcg cgcctaattt caatacattg ctggcattgc
    29461 actccaccaa tatggaagag tcgaccgaaa tcctgacgat ttttccgtgg tacagctacc
    29521 tggtcggctt atttattttt gcgctcggcg taatagcaat caggcgaaaa aaagagaatg
    29581 aaaaagcgcg ctggaatacc ttcgacagcc tgtgtctggt attcagtgtg gcgacatttt
    29641 ttgttgctcc cgtgcaaaac ctggcctggg gtggcgtatt taaactgaaa gatactggct
    29701 atccggtatt tcgttttgct aaggatgtca tcgtcaataa taacgaggtg attgaagagc
    29761 aagaacggat ggcaaaactt tccggaatga aagatacctg gacggtcact gccgttaagc
    29821 cgaagtatca gacctatgtg gtagtgatcg gtgaaagcgc gcgtcgcgat gccctcggtg
    29881 cctttggcgg tcactgggac aataccccgt ttgccagcag cgttaacggt ttgatatttg
    29941 ctgactacat tgctgccagt ggctccacgc agaaatcgct tggcttaacg ctcaatcgcg
    30001 tagtcgatgg caaaccacag tttcaggata actttgtcac cctggcaaat cgcgcgggct
    30061 tccagacctg gtggttttcc aaccagggcc aaatcggcga atacgatact gctatcgcca
    30121 gcatcgccaa acgtgcagat gaagtgtact tcctgaaaga aggtaatttt gaagcagata
    30181 aaaacacgaa agacgaagcg ttactggata tgaccgctca agtgctggcg caagagcact
    30241 cgcaaccgca gctgattgtt ctgcatctga tgggctcgca tccgcaggcc tgcgacagga
    30301 cacaaggcaa atacgaaacc tttgtgcaat cgaaagaaac gtcgtgctat ctctatacca
    30361 tgacgcaaac ggacgattta ctgcgcaagc tgtacgatca gttacgcaac agcggcagca
    30421 gcttctctct ggtttacttt tctgaccacg gtctggcctt taaagagcgc ggcaaagacg
    30481 tgcaatacct tgcccatgat gataaatatc agcaaaattt ccaggtgcct tttatggtca
    30541 tttccagcgg cgataaagcg catcgggtga ttaaagcccg ccgctcagcc aatgacttct
    30601 taggcttttt ctcgcagtgg acgggaatta aagcgaagga aataaatatc aaatacccgt
    30661 ttatatctga gaagaaagcc gggccgatat acatcaccaa cttccagtta cagaaggtgg
    30721 attacaacca tctcggaacc gatattttcg acccgaaacc ttaaaaacaa aaatccgccc
    30781 cgagaggcgg atttttatat caccaaagtg attagaagcg gtaaccaaca ccggcaatcc
    30841 aggtgcctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc agagcaacgt
    30901 tttccatcgg gttgaactgc agaccagcac cgtaggagaa accgtagtcg ctggtgtcgt
    30961 gtttgtaggt cgggtattca gtggtctgga atttaccata acccacaccc actacaccgt
    31021 agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg tagtactggt
    31081 ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg aaagaaccga
    31141 tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa ccgcccattt
    31201 tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacggta gaagtcgcag
    31261 ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga catgcaattt
    31321 ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata tatcaaaaat
    31381 taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta acagaacgtt
    31441 tccatacacc gctatccatc taaatttaaa tcactttttc agagaactgc gtaagtatta
    31501 cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa tgattccatc
    31561 aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc tcaaccagca
    31621 ttgggtatat ccagtacact ccacgcttta cttaagtcta gatatttgtg ggagaaagga
    31681 tgcctggttc attacgtaaa atgccggtct ggttaccaat agtcatattg ctcgttgcca
    31741 tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg gtgagcgcac
    31801 cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg ttctttaagc
    31861 catggcgact gcgctttgcc aaagagcaac ggttaccgct gttgttttac ggcgtttcgc
    31921 tgggtgggat gaattatctt ttttatcttt ctattcagac agtaccgctg ggtattgcgg
    31981 tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt cgcccggtag
    32041 atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt cctgctaccg ctggggcaag
    32101 acgtttccca tgtcgattta accggctgtg cgctggcact gggggccggg gcttgttggg
    32161 ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg acggtagcaa
    32221 ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag gctggtgaag
    32281 cactctggca ctggtcggtt attccattgg gtctggctgt cgttattctc tcgaccgctc
    32341 tgccttattc gctggaaatg attgccctca cccgtttgcc aacacggaca tttggtacgc
    32401 tgatgagcat ggaaccggcg ctggctgccg tttccgggat gattttcctc ggagaaacac
    32461 tgacacccat acagctgctg gcgctcggcg ctatcatcgc cgcttcaatg gggtctacgc
    32521 tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt tacatttctg
    32581 tatggttatg cataaccatg cagaatttct cgctactttt cctctacacc gtatttatat
    32641 atcgaattat gcaaaagcat atttattccg aaaattcctg gcgagcagat aaataagaat
    32701 tgttcttatc aatatatcta actcattgaa tctttattag ttttgttttt catgcttgtt
    32761 accactatta gtgtgatagg aacagccaga atagcggaac acatagccgg tgctatactt
    32821 agtctcgtta attactggga cataacatca agaggatatg aaattatgag taccgctaaa
    32881 ttagttaaat caaaagcgac caatctgctt tatacccgca acgatgtctc cgacagcgag
    32941 aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc agtttattga tctttctttg
    33001 attaccaaac aagcgcactg gaacatgcgc ggcgctaact tcattgccgt acatgaaatg
    33061 ctggatggct tccgcaccgc actgatcgat catctggata ccatggcaga acgtgccgtg
    33121 cagctgggcg gtgtagctct ggggaccact caagttatca acagcaaaac cccgctgaaa
    33181 agttacccgc tggacatcca caacgttcag gatcacctga aagaactggc tgaccgttac
    33241 gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag cgaaagatga cgacaccgca
    33301 gatatcctga ccgccgcgtc tcgcgacctg gataaattcc tgtggtttat cgagtctaac
    33361 atcgaataaa tccatcgctg atggtgcaga actttagtac ccgataaaag cggcttcctg
    33421 acaggaggcc gttttgtttt gcagcccacc tcaacgcact tatttagtgc atccatctgc
    33481 tatccccagc tgattaagta aattttttgt atccacatca tcacacaatc gttacataaa
    33541 gattgttttt tcatcaggtt ttacgctaaa taatcactgt gttgagtgca caattttagc
    33601 gcaccagatt ggtgccccag aatggtgcat cttcagggta ttgccctata aatcgtgcat
    33661 cacgtttttg ccgcatctcg aaaaatcaat gagttgcaaa attggcacga ttttttcata
    33721 tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa aaaggaaatg ctatgaagtc
    33781 tgtattaaaa gtttcactgg ctgcactgac cctggctttt gcggtttctt ctcatgccgc
    33841 ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt ccgtttgaat ttaaacaggg
    33901 cgataaatat gtgggctttg acgttgatct gtgggctgcc atcgctaaag agctgaagct
    33961 ggattacgaa ctgaagccga tggatttcag tgggatcatt ccggcactgc aaaccaaaaa
    34021 cgtcgatctg gcgctggcgg gcattaccat caccgacgag cgtaaaaaag cgatcgattt
    34081 ctctgacggc tactacaaaa gcggcctgtt agtgatggtg aaagctaaca ataacgatgt
    34141 gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg aagagcggta ctggctccgt
    34201 tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt cagttcccga acatcgataa
    34261 cgcctatatg gaactgggca ccaaccgcgc agacgccgtt ctgcacgata cgccaaacat
    34321 tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa gcggtaggtg actctctgga
    34381 agcgcagcaa tacggtattg cgttcccgaa aggtagcgac gagctgcgtg acaaagtcaa
    34441 cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac gaaatctaca aaaaatggtt
    34501 cagtactgaa ccgaaataat aacgctacac ctgtaaagcg cactggcagt tccctctccc
    34561 ccctggggag aggattaggg tgaggggcgc aaacccgctc cggggccatt aattaccctg
    34621 aatttgatta tttacaccac ggtaacagga acaacatatg cagtttgact ggagtgccat
    34681 ctggcctgcc attccgcttc tgattgaagg tgccaaaatg accctgtgga tttcggtcct
    34741 cggtctggct ggcggtctgg taatcggatt gctggcaggt tttgcacgca ccttcggtgg
    34801 ttggatagcc aaccacgtcg cgctggtctt tattgaagtg atccgcggca cacctatcgt
    34861 cgtccaggtg atgtttatct atttcgccct gccgatggcg tttaacgact tacgcatcga
    34921 cccatttact gcggcggtgg tcaccatcat gatcaactcc ggcgcgtaca ttgcggaaat
    34981 cacgcgtggt gcggtgctgt ctatccacaa aggttttcgt gaagcaggac tggcgctcgg
    35041 tctttcacgt tgggaaacca ttcgctacgt cattttaccg ctggcactgc gtcgtatgct
    35101 gccgccgctg ggtaaccagt ggatcatcag cattaaagac acctcgctgt ttattgttat
    35161 cggcgtggcg gaactgaccc gtcaggggca agaaattatt gccggtaact tccgcgccct
    35221 tgagatctgg agcgccgtgg cggtgttcta tctgattatt accctggtgc tgagctttat
    35281 tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg aatttaaaaa cgtctccaag
    35341 cactttggcc caacccaggt gctgcacaat atcgatttga acattgccca gggcgaagtc
    35401 gtggtgatta tcgggccgtc cggttccggt aaatcgaccc tgctgcgctg catcaacaaa
    35461 ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc tgaaggtcaa cgatccgaaa
    35521 gttgacgagc gcctgattcg ccaggaagca ggtatggtgt tccagcagtt ttacctcttc
    35581 ccgcatctga cggcgctggg aaacgtcatg tttggtccgc tacgcgtgcg tggcgcgaac
    35641 aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga aagtcggtct ggcagaacgt
    35701 gcacatcact acccttccga actttctggt ggtcaacagc agcgtgtggc gattgcccgc
    35761 gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac cgacttccgc tcttgacccg
    35821 gaactgcgcc atgaagtgct gaaggttatg caggatctgg ctgaagaagg gatgacgatg
    35881 gtgatcgtga cccacgaaat cggttttgcc gagaaagtag cttcgcggct gatctttatc
    35941 gacaaaggcc ggattgcgga agatggcaat ccgcaggtgt tgatcaagaa cccgccgagc
    36001 cagcgcttgc aggaattttt gcagcacgtc tcttaataag acacattgcc tgatgcgcta
    36061 cgcttatcag gcctacagga tatctggcaa cttattaaaa ttgcatgaac ttgtaggacg
    36121 gataaggcgt tcacgccgca tccggcaaaa aagcccgcac gttgtcagca acctgcttaa
    36181 tatcccttcc tccctttcac ccgaaaggga ggcacaccag attcctctca tttaaaatcg
    36241 cccctccccc agcatctata cttatctttt tgctctgttt tctcactgga ggagtcatgc
    36301 ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg gtatccatac
    36361 ccggcgttac aaccacaacg acaacggact caacgactga accggccccg gaaccggata
    36421 tcgaacaaaa aaagcggcct atggcgcact ggcggatgtg ctggataacg acacctcgcg
    36481 taaagagttg atcgaccagt tgcgtactgt cgccgcgacg ccccctgcgg aaccggtccc
    36541 gaagatcgtg ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt
    36601 cagccgccat tatggtgaag ccctttccgc ccgcttcggg caactttatc gcaatatcac
    36661 cggctccccg cataagccat ttaatccaca aaccttcagc aatgcgctga gccatttttc
    36721 aatgttagcg gtattagtgt ttggttttta ctggctgatt cgcctgtgcg cactgccgct
    36781 atatcgcaaa atgggtcagt gggcgaggca aaaaaatcgt gagcgcagca actggttgca
    36841 gcttccggcg atgattattg gggcgtttat tatcgacctg ctgttactgg ccctgacatt
    36901 atttgtcggc caggtattaa gcgacaacct gaatgcaggc agtcgcacca tcgctttcca
    36961 acaaagtttg tttctcaacg cctttgccct gattgaattt ttcaaagccg tactacgcct
    37021 gattttttgc ccaaacgtgg cggagctgcg cccgttcacg attcaggacg agagcgcccg
    37081 ttactggggt cgtcgcctga gctggttaag cagcctgatt ggctatggcc tgattgtggc
    37141 cgtgccgatt atctctaatc aggtgaatgt acagataggt gcgctggcga acgtcatcat
    37201 tatgctgtgc atgaccgtct gggcgttgta cctgatcttc cgaaataaaa aagagattac
    37261 ccagcatatg ctcaactttg cggagcattc gctggctttt ttcagcctgt ttatccgcgc
    37321 ctttgcgctg gtgtggcact ggctggcaag cgcctatttt atcatgctgt ttttcttttc
    37381 gttgttcgat ccgggcaaca gtctgaaatt tatgatgggt gcaacggtgc gcagcctggc
    37441 gattattggt atcgcagcgt ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat
    37501 caccctctcg ccacatactc agcgtaacta tccggagcta caaaaacggc tgaatggctg
    37561 gctttcggcg gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcag taatgttgct
    37621 gttgagcgca tggggattgt tcgatttctg gaactggctg caaaacggcg cggggcagaa
    37681 aaccgtagat atcctgatcc gcatcgcact cattcttttc ttctcggcgg ttggctggac
    37741 ggtgctcgcc agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccgctacc
    37801 cagcgcccgt acgcgtaccc tgctgacgct gttccgtaac gcgctggcgg tgattatcag
    37861 taccatcacc atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc
    37921 aggtgccggg gcattaggtc tggctatctc gtttggttcg caaacgctgg taaaagatat
    37981 tatcaccggg gtatttattc agtttgaaaa cggcatgaac actggagatt tggtgactat
    38041 cgggccgttg accggcacag tggaacggat gtcgattcgc tccgtgggcg tgcgccagga
    38101 taccggggcg tatcacatca ttccgtggtc ttcgataacc acctttgcta acttcgtccg
    38161 cggtattggt tcggtagtgg caaactacga tgttgatcgc catgaagatg ctgataaagc
    38221 caatcaggca ctgaaagatg cggtaacgga attgatggaa aacgaagaaa ttcgcgggct
    38281 gattattggt gaaccgaact ttgccgggat tgtcggctta agcaataccg cgtttacact
    38341 gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg gtacgctttg ccctcgacag
    38401 ccaggtgaaa aaacatttcg acctggcggg cgttcgcgcg ccagtgcaga cttatcaggt
    38461 gctgcctgct ccgggcgcga ccccggctga accgctgccg ccgggggagc caacgcttta
    38521 acgttggcga ttaacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa
    38581 gcgactctgc ttttgcccct gggccatctc tttttttacc accttcaccg cgcccacgtc
    38641 ggtcagagca cgatacaacg gcggtaagtt ttcaccacga gataccagcg aggtaaacca
    38701 catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa agttgacttc
    38761 accgccttca caccacaact cctgttgctg accaccaaag ttcagtgcat cgtctttgtt
    38821 cagccccagg ttacggcgtt tacgctcact acctgcccgc gccgcagcgg cggaatcgtg
    38881 gaatggcggg ttacacaagg tcgcgtcgta ttgctcgttt ttgtggatga tgccgttaaa
    38941 aatcgcccca ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattagc
    39001 actgataatc gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg
    39061 ccagccatat tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa cgccgatatc
    39121 cagaatgctg gcattcgctg gaattgttcc gcttgcttca gccagtaaat cggcaaggtg
    39181 atgaatataa tccgcccggc ccggtactgg tgggcagaga aaaccgtcgg ggatatccca
    39241 gttcgctacg gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg
    39301 attggcaaag tctacgcttt gctcccctgc gggtgtaagc gtgaggaatt gcctgagttc
    39361 aggattgacc tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg
    39421 caaccccggt ttctgggcgg acatagcatt ctcctttaag cagcggcgta agatacccgc
    39481 ataacgcgac agggtaaata tctggctgcg tttatcattc cccaaaacct taacatggct
    39541 atggtttatg gcaattcatc ataaaaatca gcattatgca tgtcattgat ttgtcttatt
    39601 ttgtaaaaat atatcgttca aaaaataagc atttctctgt caattttttg cgtcatttgc
    39661 atcgcgcagg cgacctacac ttatcaggca ctacccgttg tatttatgag gatgtcatta
    39721 tgaaaaagtg cctcacacta ctgattgcca ccgtcctgag cggaatctct ctcacggctt
    39781 atgccgcgca accgatgagt aacctggaca gcggtcagct acggcccgcc ggtaccgttt
    39841 cggcaactgg cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg
    39901 aacaaggcgc gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttaggta
    39961 ctgcaaccat ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt
    40021 cgcctgtttt gtaatcaaaa aacatcaaat atgaacgcaa tgtaaataaa tgtatttctt
    40081 tttcgcgcaa tgggtgatag aaaatcgcat caagtgataa tgcttatcaa aattattatc
    40141 acttgcacga acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc
    40201 gtggcagtga aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca
    40261 atttccctgc cagacaattt cattcgctca cgttctttgc cggtctttgt attggtatta
    40321 cgcctgtggc acaggcactc gccgccgaag ggcaagctaa cgcggatgac acgctggttg
    40381 tcgaagcatc aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc
    40441 gtccggtagc ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc
    40501 agggcgcaac caaccttacc gacgcgctca aaaacgtccc cggcg
//
LOCUS       DAZOZX010000023        40171 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZOZX010000023 DAZOZX010000000
VERSION     DAZOZX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.023"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            244..330
                     /gene="leuL"
                     /locus_tag="ACHUPT_001178"
     CDS             244..330
                     /gene="leuL"
                     /locus_tag="ACHUPT_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0818831.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            423..1994
                     /gene="leuA"
                     /locus_tag="ACHUPT_001179"
     CDS             423..1994
                     /gene="leuA"
                     /locus_tag="ACHUPT_001179"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0818832.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            1994..3085
                     /gene="leuB"
                     /locus_tag="ACHUPT_001180"
     CDS             1994..3085
                     /gene="leuB"
                     /locus_tag="ACHUPT_001180"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0818833.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3088..4488
                     /gene="leuC"
                     /locus_tag="ACHUPT_001181"
     CDS             3088..4488
                     /gene="leuC"
                     /locus_tag="ACHUPT_001181"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0818834.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4499..5104
                     /gene="leuD"
                     /locus_tag="ACHUPT_001182"
     CDS             4499..5104
                     /gene="leuD"
                     /locus_tag="ACHUPT_001182"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0818835.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5153..6331)
                     /gene="setA"
                     /locus_tag="ACHUPT_001183"
     CDS             complement(5153..6331)
                     /gene="setA"
                     /locus_tag="ACHUPT_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0818836.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6433..6564)
                     /gene="sgrT"
                     /locus_tag="ACHUPT_001184"
     CDS             complement(6433..6564)
                     /gene="sgrT"
                     /locus_tag="ACHUPT_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0818837.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6653..8308
                     /gene="sgrR"
                     /locus_tag="ACHUPT_001185"
     CDS             6653..8308
                     /gene="sgrR"
                     /locus_tag="ACHUPT_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0818838.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8342..8441
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8472..9455
                     /gene="thiB"
                     /locus_tag="ACHUPT_001186"
     CDS             8472..9455
                     /gene="thiB"
                     /locus_tag="ACHUPT_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0818839.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9431..11041
                     /gene="thiP"
                     /locus_tag="ACHUPT_001187"
     CDS             9431..11041
                     /gene="thiP"
                     /locus_tag="ACHUPT_001187"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0818840.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11025..11723
                     /gene="thiQ"
                     /locus_tag="ACHUPT_001188"
     CDS             11025..11723
                     /gene="thiQ"
                     /locus_tag="ACHUPT_001188"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0818841.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11837..12601)
                     /locus_tag="ACHUPT_001189"
     CDS             complement(11837..12601)
                     /locus_tag="ACHUPT_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0818842.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12687..13565)
                     /gene="araC"
                     /locus_tag="ACHUPT_001190"
     CDS             complement(12687..13565)
                     /gene="araC"
                     /locus_tag="ACHUPT_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0818843.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13904..15604
                     /gene="araB"
                     /locus_tag="ACHUPT_001191"
     CDS             13904..15604
                     /gene="araB"
                     /locus_tag="ACHUPT_001191"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0818844.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15615..17117
                     /gene="araA"
                     /locus_tag="ACHUPT_001192"
     CDS             15615..17117
                     /gene="araA"
                     /locus_tag="ACHUPT_001192"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0818845.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17317..18012
                     /gene="araD"
                     /locus_tag="ACHUPT_001193"
     CDS             17317..18012
                     /gene="araD"
                     /locus_tag="ACHUPT_001193"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0818846.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLHKH
                     YLRKHGAKAYYGQ"
     gene            18087..20438
                     /gene="polB"
                     /locus_tag="ACHUPT_001194"
     CDS             18087..20438
                     /gene="polB"
                     /locus_tag="ACHUPT_001194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0818847.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20603..23509
                     /gene="rapA"
                     /locus_tag="ACHUPT_001195"
     CDS             20603..23509
                     /gene="rapA"
                     /locus_tag="ACHUPT_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0818848.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23521..24180
                     /gene="rluA"
                     /locus_tag="ACHUPT_001196"
     CDS             23521..24180
                     /gene="rluA"
                     /locus_tag="ACHUPT_001196"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0818849.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24297..25112)
                     /gene="djlA"
                     /locus_tag="ACHUPT_001197"
     CDS             complement(24297..25112)
                     /gene="djlA"
                     /locus_tag="ACHUPT_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0818850.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25367..27721
                     /gene="lptD"
                     /locus_tag="ACHUPT_001198"
     CDS             25367..27721
                     /gene="lptD"
                     /locus_tag="ACHUPT_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0818851.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27774..29060
                     /gene="surA"
                     /locus_tag="ACHUPT_001199"
     CDS             27774..29060
                     /gene="surA"
                     /locus_tag="ACHUPT_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0818852.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29060..30049
                     /gene="pdxA"
                     /locus_tag="ACHUPT_001200"
     CDS             29060..30049
                     /gene="pdxA"
                     /locus_tag="ACHUPT_001200"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0818853.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30046..30867
                     /gene="rsmA"
                     /locus_tag="ACHUPT_001201"
     CDS             30046..30867
                     /gene="rsmA"
                     /locus_tag="ACHUPT_001201"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0818854.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLTARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30870..31247
                     /gene="apaG"
                     /locus_tag="ACHUPT_001202"
     CDS             30870..31247
                     /gene="apaG"
                     /locus_tag="ACHUPT_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0818855.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31254..32096
                     /gene="apaH"
                     /locus_tag="ACHUPT_001203"
     CDS             31254..32096
                     /gene="apaH"
                     /locus_tag="ACHUPT_001203"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0818856.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32174..32653)
                     /gene="folA"
                     /locus_tag="ACHUPT_001204"
     CDS             complement(32174..32653)
                     /gene="folA"
                     /locus_tag="ACHUPT_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0818857.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32844..34706)
                     /gene="kefC"
                     /locus_tag="ACHUPT_001205"
     CDS             complement(32844..34706)
                     /gene="kefC"
                     /locus_tag="ACHUPT_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0818858.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34699..35229)
                     /gene="kefF"
                     /locus_tag="ACHUPT_001206"
     CDS             complement(34699..35229)
                     /gene="kefF"
                     /locus_tag="ACHUPT_001206"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0818859.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35277..36671)
                     /locus_tag="ACHUPT_001207"
     CDS             complement(35277..36671)
                     /locus_tag="ACHUPT_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0818860.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36730..37017)
                     /gene="fixX"
                     /locus_tag="ACHUPT_001208"
     CDS             complement(36730..37017)
                     /gene="fixX"
                     /locus_tag="ACHUPT_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0818861.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37014..38300)
                     /locus_tag="ACHUPT_001209"
     CDS             complement(37014..38300)
                     /locus_tag="ACHUPT_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0818862.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38351..39292)
                     /locus_tag="ACHUPT_001210"
                     /pseudo
     CDS             complement(38351..39292)
                     /locus_tag="ACHUPT_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39307..40077)
                     /locus_tag="ACHUPT_001211"
     CDS             complement(39307..40077)
                     /locus_tag="ACHUPT_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0818863.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9647 a        11105 c        10557 g         8862 t
ORIGIN      
        1 attttgtccc ctttttcctc gttgattaga tgcaaaaatt tatgctgaaa tatgtcaacc
       61 gatgaaaagc gtcggtagtt aagcagaaat taatatcgct tactttaacc accgtagcac
      121 aattagctaa ttttacggat gcagaactca cgctggcggg acgtttttat tgcgtcaggg
      181 ttgacatccg tttttgtatc cagtaactct aaaagcatat cgcattcatc tggagctgat
      241 ttaatgactc acatcgttcg ctttatcggt ctactactac taaacgcatc ttctttgcgc
      301 ggtagacgag tgagcggcat ccagcattaa gccagcacgc agtcaaacaa aaaacccgcg
      361 ccattgcgcg ggttttttta tgcccgaagc gaggcgctct aaaagagaca aggacccaaa
      421 ccatgagcca gcaagtcatt attttcgata ccacattgcg cgacggtgaa caggcgttac
      481 aggcaagctt gagtgtgaaa gaaaaactgc aaattgcgct ggcccttgag cgtatgggtg
      541 ttgacgtgat ggaagtcggt ttccccgtct cttcgccggg cgattttgaa tcggtgcaaa
      601 ccatcgcccg ccaggttaaa aacagccgcg tatgtgcgtt agctcgctgc gtggaaaaag
      661 atatcgacgt ggcggccgaa tccctgaaag tcgccgaagc cttccgtatt cataccttta
      721 ttgctacctc cccaatgcac atcgccacca agctgcgcag caccctagac gaggtgatcg
      781 aacgcgctat ctatatggtg aaacgcgccc gtaattacac cgatgatgtt gaattttctt
      841 gcgaagatgc cgggcgtaca cccattgccg atctggcgcg agtggtcgaa gcggcgatta
      901 atgccggtgc caccaccatc aacattccgg acaccgtggg ctacaccatg ccgtttgagt
      961 tcgccggaat catcagcggc ctgtatgaac gcgtacctaa catcgacaaa gccattattt
     1021 ccgtacatac ccacgacgat ttaggcctgg cggtcggcaa ctcactggcg gcggtacatg
     1081 ccggtgcacg ccaggtggaa ggtgcaatga acgggatcgg cgagcgtgcc ggtaactgtt
     1141 cgctggaaga agtcatcatg gcgattaaag ttcgtaagga tattctcaac gtccacaccg
     1201 ccattaatca ccaagagata tggcgcacca gccagttagt tagccagatt tgtaatatgc
     1261 cgatcccggc aaacaaagcc attgttggca gcggcgcatt cgctcactcc tccggtatcc
     1321 accaggatgg cgtgctgaaa aaccgcgaaa actacgaaat catgacacca gaatctattg
     1381 gtctgaacca aatccagctg aatctgacct ctcgttcggg gcgtgcggcg gtgaaacatc
     1441 gcatggatga gatggggtat aaagaaagtg aatataattt agacaatttg tacgatgctt
     1501 tcctgaagct ggcggacaaa aaaggtcagg tgtttgatta cgatctggag gcgctggcct
     1561 tcatcggtaa gcagcaagaa gagccggagc atttccgtct ggattacttc agcgtgcagt
     1621 ctggctctaa cgatatcgcc accgccgccg tcaaactggc ctgtggcgaa gaagtcaaag
     1681 cagaagccgc caacggtaac ggtccggtcg atgccgtcta tcaggcaatt aaccgcatca
     1741 ctgactataa cgtcgaactg gtgaaataca gcctgaccgc caaaggtcac ggtaaagatg
     1801 cgctgggtca ggtggatatt gtcaccaact acaacggtcg ccgcttccac ggcgtcggcc
     1861 tggccaccga tattgtcgag tcctccgcca aagccatggt gcacgtactg aacaatatct
     1921 ggcgtgccgc agaagtcgaa aaagagttgc aacgcaaaac tcaacacaac gaaaacaaca
     1981 aggaaaccgt gtgatgtcga agaattacca tattgccgta ttgccgggag acggtattgg
     2041 tccggaagtg atgacccagg cgctgaaagt gctgaatgcc gtgcgcaacc gctttgcgat
     2101 gcgcatcacc accagccatt acgatgtagg cggcgcagcc attgataacc acgggcaacc
     2161 actgccgcct gcgacggttg aaggttgtga gcaagccgat gccgtgctgt ttggctcggt
     2221 aggcggcccg aagtgggaac atttaccacc agaccagcaa ccagaacgcg gcgcgctgct
     2281 gccgctgcgt aagcacttca aattattcag caacctgcgc ccggcaaaac tgtatcaggg
     2341 gctggaagca ttctgtccgc tgcgtgcaga cattgccgct aacggcttcg acatccagtg
     2401 cgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca aaaggccgcg aaggtagcgg
     2461 acaatatgaa aaagcgtttg ataccgaggt gtatcaccgt tttgagatcg agcgtattgc
     2521 ccgcatcgcg tttgaatctg ctcgtaaacg tcgccacaaa gtcacctcaa tcgacaaagc
     2581 taacgtgctg caatcctcta ttttatggcg tgaaatcgtc aacgagatcg ccacggaata
     2641 cccggatgtc gaactggcgc atatgtacat cgacaacgcc accatgcagc tgattaaaga
     2701 tccatcacag tttgacgttc tgctgtgctc caacctgttt ggcgacattc tgtctgacga
     2761 gtgcgcaatg atcactggct cgatgggcat gttgccttcc gccagcctga acgagcaagg
     2821 ttttggactg tatgaaccgg cgggcggctc ggcaccagat atcgcaggca aaaacatcgc
     2881 caacccgatt gcacaaatcc tgtcgctggc actgctgctg cgttacagcc tggatgccga
     2941 tgatgcggct tccgccattg aacgcgccat taaccgtgca ttagaagaag gcattcgcac
     3001 cggggattta gcccgtggcg ctgccgccgt tagtaccgat gaaatgggcg atatcattgc
     3061 ccgctatgtg gcagaagggg tgtaatcatg gctaaaacgt tatacgaaaa attattcgac
     3121 gctcacgtag tgtacgaagc cgaaaacgaa accccgctgt tatatatcga ccgccacctg
     3181 gtgcatgaag tgacctcacc gcaggcgttc gatggtctgc gcgcccacgg tcgcccggta
     3241 cgtcagccgg gcaaaacctt cgccactatg gatcacaacg tctctaccca gaccaaagac
     3301 attaatgcct gtggtgaaat ggcgcgcatc cagatgcagg agctgattaa aaactgcaaa
     3361 gaatttggcg tcgagctgta tgacctgaat cacccgtatc aggggatcgt ccacgtaatg
     3421 gggccggaac agggcgtcac cttgccgggg atgaccattg tctgcggcga ctcgcatacc
     3481 gccacccacg gcgcgtttgg cgcactggcc tttggtatcg gcacttccga agttgaacac
     3541 gtactggcaa cacaaaccct gaaacagggc cgtgcgaaga ccatgaaaat tgaagtccag
     3601 ggcaaagccg cgccgggcat taccgcaaaa gatatcgtgc tggcaattat cggtaaaact
     3661 ggcagcgcag gcggcaccgg gcatgtggtg gagttttgcg gcgaagcaat tcgtgattta
     3721 agcatggaag gtcgtatgac cctgtgcaat atggcgatcg aaatgggcgc aaaagccgct
     3781 ctggttgctc ctgacgaaac cacctttaac tatgtcaaag gccgtctgca tgcgccgaaa
     3841 ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc tgcaaaccga cgaaggcgca
     3901 actttcgata ccgttgtcac tctgcaagca gaagaaattt caccgcaggt cacctggggc
     3961 accaatcccg gccaggtgat ttctgtgaac gacaatattc ccgatccggc ttcgtttgcc
     4021 gatccggttg aacgtgcgtc ggcagaaaaa gcgctggcct atatggggct gaaaccgggt
     4081 attccgctga ccgaagtggc tatcgacaaa gtgtttatcg gttcctgtac caactctcgc
     4141 attgaagatt tacgcgcggc agcggagatc gccaaagggc gaaaagtcgc gccaggcgtg
     4201 caggcactgg tggttcccgg ctctggcccg gtaaaagccc aggcggaagc ggaaggtctg
     4261 gataaaatat ttattgaagc cggttttgaa tggcgcttgc ctggctgctc aatgtgtctg
     4321 gcgatgaaca acgaccgtct gaatccgggc gaacgttgtg catccaccag caaccgtaac
     4381 tttgaaggcc gccaggggcg cggcgggcgc acgcatctgg tcagcccggc aatggctgcc
     4441 gctgctgctg tgaccggaca tttcgccgac attcgcaaca ttaaataagg agcacaccat
     4501 ggcagagaaa tttatcaaac acacaggcct ggtggttccg ctggatgccg ccaatgtcga
     4561 taccgatgca atcatcccga aacagttttt gcagaaagtg acccgtacgg gttttggcgc
     4621 gcatctgttt aacgactggc gttttctcga tgaaaaaggc caacagccaa acccggactt
     4681 cgtgctgaac ttcccgcagt atcagggcgc ttccattttg ctggcacgag aaaacttcgg
     4741 ctgtggctcc tcgcgtgagc acgcgccctg ggcattgacc gactacggtt ttaaagtggt
     4801 gattgcgccg agttttgctg acatcttcta cggcaatagc tttaacaacc agctgctgcc
     4861 ggtgaaatta agcgatgcag aagtggacga actgtttgcg ctggtgaaag ccaatccggg
     4921 gatccatttc gacgtggatc tggaagcgca agaggtgaaa gcgggagaga aaacctatcg
     4981 ctttaccatc gatgccttcc gccgccactg catgatgaat ggtctggaca gcatcggcct
     5041 gaccctgcag cacgacgacg ctattgcgtc ttatgaagca aaacaacctg cgtttatgaa
     5101 ttagacccct tgcccggtca aatgaccggg ctttccgcta tcgtccacgt catcaaacgt
     5161 ctttaacctt tgcggttaaa aataatgcga caacagaaat aatcgcaatt acccagtaga
     5221 cagcaaagtg cccccaactt tgtgcaattg ctccctgaat aacaccagcc agaattaccc
     5281 cggtagagat agtgttggta aataaggtgg tagctgcccc cgctcttcca ggcattaaat
     5341 cctgaaacca tagcatccca atacccgcaa caatgccgat aaatacagcg ttaaaaagtt
     5401 gcagcgtcat caacgccata cggctatgaa agaaaatcaa tccagtgtaa aacagcactc
     5461 ctgccaccac tgcaatgacc atcattcgcc gcttaccata acgtttgaca tagtagccag
     5521 ccagaatcat tgctggtatt tccagtccag ctgccgtccc catcaggaaa cccgccagtt
     5581 tgtctggcaa tcctaaatcg ctactgatcc acaatggcat atcaataatg tacatagtgt
     5641 tgcaggtcca cattaacgtc gaggcgacaa ataacatccg tacgttacta tcctgccagt
     5701 caccttgcat tgataaagca ttttccgacg gcagttctac ccgcgccaca gacagaagca
     5761 taaatgcaat caataccaga ctgagtgtga atatcccggc ggcaatcgaa aacatcactg
     5821 taaagccgta attcaacgcc agcataaagg ccagcggtgg accgattacc catgccagag
     5881 aaagctgcgc acgcatcacc gagctaaaca tcaccacttc tcgcgccgag ttatccgcgt
     5941 attcccgcgc cagagcaaat aactgtggca ttgccgtatt ggccagagat gccagaagca
     6001 caccacaggt gataagcgtc agataatgac gattaaatgc aaacagtagc gcattgccaa
     6061 tagccatcaa acagcaaaat ataatcagtt ttcgccgatc gccctgactg tcagaacgtt
     6121 ttgccaacca gaggcttacg ccgatcccag caatagcatt caccgtataa aagagtccga
     6181 tccagaaagg ttgcgcgcca acctcacgac tcagaaataa gctcaatgta ggagcctgta
     6241 gcgccccggc cacccccatc ataaaagcga ccagcataaa tgccgcgtaa acaccgttca
     6301 tacggcgagc catcgtcatt atccagatca tacgttccct ttttagcgcg gcgagaataa
     6361 aaaaaaccag caggtataat ctgctggcgg gtgattttac accaatactc agtcacacat
     6421 gatgcaggca agtcaacttt cagaattgcg gtcatcccac tgcatcagtc cttcaagcat
     6481 ggttaatcgt tgtgggacgc ttaaccaacg caaccagcac aacttcgctg tcgcggtaaa
     6541 atagtgctga taaaactgac gcatggggca cctccttgct tcatcgttag gaattattga
     6601 cttaatatag ggaaaataaa attgctgtct tttgcgcagg agttcccctt ttatgccatc
     6661 tgctcgtctg caacaacagt tcatccgcct gtggcaatgc tgcgagggta aatcgcagga
     6721 cacaacgctc aacgaactgg cagcgttatt gagctgctcg cgtcgtcata tgcgtaccct
     6781 gctcaacacc atgcaggatc gcggctggct gacgtgggaa gcggaagtcg ggcgcggtaa
     6841 acgctcgcgt ctgacattcc tctataccgg gctggcgctt cagcaacagc gggcggaaga
     6901 cctgctggag caggatcgta tcgaccaact agtgcagttg gttggcgaca aagcgactgt
     6961 gcggcaaatg ctggtttctc atctgggccg cagcttccgc caggggcggc acatcctgcg
     7021 cgtgctctac tatcgtccgt tgcgtaatct gctacctggc agcgcattgc gccgttccga
     7081 aacccatatt gcccggcaaa tcttcagttc gctaacgcgc ataaatgagg aaaatgggga
     7141 actggaagca gacatcgccc accactggca gcaaatatca ccgcttcact ggcgtttctt
     7201 tttgcgtcca ggagtccatt ttcatcatgg tcgtgaactg gaaatggacg acgtgatcgc
     7261 ctctttaaaa cgaatcaata cgctgccgct ctattcgcat attacggaca ttgtgtcgcc
     7321 gacgccctgg acgctggata tccatctcac gcagccggac cgctggttgc cgttactgct
     7381 ggggcaagtt ccggcgatga tcctgccgtg cgaatgggaa accctcagta actttgccag
     7441 ccatcctatt ggcaccggtc cgtatgcggt gattcgcaac agcaccaatc aactgaaaat
     7501 tcaggcattc gatgacttct tcggttaccg ggcattaatc gacgaagtta acgtctgggt
     7561 tctgccggaa attgccgacg agccagccgg tggtctgatg ctaaaaggtc cacagggcga
     7621 gaaaaaagag attgaaagcc gcctggagga aggttgctac tatttactgt tcgacagccg
     7681 cacccatcgc ggggcgaatc agcaagtcag ggactgggta agctatgtgc tttctccaac
     7741 taatctggtc tatttcgctg aggaacagta ccagcaactg tggttcccgg cttatggact
     7801 gctcccccgt tggcaccatg cccgcaccat aaagagcgaa aaaccggctg gcctggaaag
     7861 cctcaccctg accttttatc aggatcacag tgagcatcgg gtgattgccg ggatcatgca
     7921 gcagattctg gcaagtcacc aggtcacgct ggaaatcaaa gagatcagct acgatcagtg
     7981 gcatgaagga gagatcgaga gcgatatctg gcttaatagc gccaacttta cgctgccgct
     8041 ggatttttcg ctgttcgcgc acttgtgcga ggtgccgctg ctccaacact gtattccgat
     8101 cgactggcaa gccgacgccg cccgctggcg caatggcgaa atgaacctgg cgaactggtg
     8161 ccagcaactg gtcgccagta aagcaatggt gccgcttatc caccactggc tgatcattca
     8221 gggacaacgc agtatgcgcg gcctgcgtat gaataccctc ggctggttcg attttaaatc
     8281 agcgtggttt gcgccaccgg atccatgatt gctggtagat aacaaaatca ctacactaac
     8341 gccgttctca acggggtgcc acgcaaacgc gtgcgctgag aaaatacccg tcgaacctga
     8401 tccggataac gccggcgaag ggatttgagg ctccttctca agtcctttgc cactcttttt
     8461 tgaggtgcaa agtgttaaaa aaatgtcttc ccctgctgtt gctgtgcaca gcgcccgttt
     8521 tcgctaaacc cgttctgact gtttatacct acgattcctt cgccgccgac tgggggcctg
     8581 gtccaaagat caaaaaagct tttgaagcca actgtaactg cgaactgaaa ctggtggcgc
     8641 tggaagatgg cgtttcgctt ctcaaccgtc tacggatgga aggcaaaaat agtaaagccg
     8701 atgtggtgct ggggctggat aacaatctgt tagacgccgc cagtaaaacc ggactatttg
     8761 ccaaaagcgg tgtggcagcg gatgccgtta acgttcccgg cggctggaat aatgacactt
     8821 tcgtcccgtt tgattacggc tatttcgcct tcgtctatga caagaacaaa ctgaaaaacc
     8881 cgccgcaaag cctgaaagaa ttggttgaga gcgatcagaa ctggcgggtg atttatcagg
     8941 atccgcgtac cagcacgccg gggctgggtc tgctgttgtg gatgcaaaaa gtctatggcg
     9001 atgacgcccc acaagcctgg caaaaactgg cgaagaaaac cgtcacggtc accaaaggct
     9061 ggagcgaagc ctacggcctg tttttaaaag gtgaaagcga tctggtactg agttacacca
     9121 cctctccggc ttatcacatt ctcgaagaga aaaaagataa ctacgccgcc gcgaacttta
     9181 gcgaaggtca ctatctgcag gtggaagtcg ccgcccgcac cgctgccagc aagcagccgg
     9241 agctggcgca aaaattcctc cagtttatgg tttctccggc ttttcagaat gcgatcccaa
     9301 ccggtaactg gatgtatccg gtggcaaatg tcacgctgcc tgccgggttt gaacaattga
     9361 ccaaaccggc caccacgctg gagttcacac cagctgaagt ggcggcacaa cgtcaggcat
     9421 ggattagcga atggcaacgc gccgtcagcc gttaattccc ggctggttaa ttccaggtgt
     9481 aagcgccgcc acgctggtgg tagcggttgc gctcgcggcg tttctcgccc tgtggtggaa
     9541 cgcgccgcag ggtaactggg tggcagtctg gcaggacagc tacctgtggc atgtggtgcg
     9601 cttctccttc tggcaggcgt ttctctcggc gctactctct gtcgtacccg cgatattcct
     9661 cgcccgcgcc ctctatcgca ggcgctttcc gggtcggctg gcgctgttgc gtctgtgcgc
     9721 aatgaccttg atcctcccgg tgctagttgc tgttttcggc attcttagcg tctatggtcg
     9781 tcagggctgg ctggcatcgc tctgccaatc gctcggtctg gagtggacct tttcgcccta
     9841 cggcctgcaa ggcattttgc tggcgcacgt attttttaat ctgccgatgg cgagccgctt
     9901 attactccag gcactggaaa acattcccgg cgaacaacgt cagcttgccg cccagcttgg
     9961 gatgcgcggc tggcattttt tccgcttcgt cgaatggccg tggctacggc gacaaatccc
    10021 gccggttgcc gcgcttatct ttatgctctg tttcgccagc ttcgccaccg tgctatcgct
    10081 gggcggcggt ccgcaggcaa ccactatcga gctggctatt tatcaggcgc tgagttacga
    10141 ctacgatcct gcccgcgcgg cgatgctggc gctgctccag atggtgtgtt gccttgggct
    10201 ggtgctgttg agtcagcgac taagtaaggc cattgcgccc ggcaccacgc tgctgcaagg
    10261 ctggcgcgat ccggacgatc gtctgcatag ccgcatttgc gacacggtgt taatagtgct
    10321 ggcgctgctg ctgttgctgc caccgttgct ggcggtgatc gtcgatgggg taaatcgcca
    10381 gttgccggaa gtgctggcac aaccggtgct gtggcaggcg ctgtggacct cgttgcgtat
    10441 tgcgctggcg gcaggtgtat tgtgcgtagt gctgaccatg atgctgctat ggagcagtcg
    10501 cgaactgcgg gcgcggcaga aaatgctggc gggtcaggcg ctggagatga gcggcatgtt
    10561 gatcctcgcc atgccgggga ttgtgctggc taccggcttc tttttactgc tcaacaacac
    10621 tatcggcctg ccacaatctg ctgacggcat tgtgattttc accaatgcgt taatggcgat
    10681 cccttatgcg ctgaaagtac tggaaaaccc gatgcgcgat atcaccgccc gctacagtat
    10741 gttgtgtcag tcgctgggca ttgaaggctg gtcgcgctta aaagtggtcg agctgcgcgc
    10801 cctgaaacgt ccactggcgc aggcgctggc ttttgcctgc gtgctgtcga ttggtgattt
    10861 tggcgtagtg gcgttgttcg gtaacgatga tttccgtacc ctgccgtttt atctctacca
    10921 gcaaattggc tcctatcgca gccaggacgg cgcggtcacc gcgttaattc tgctactact
    10981 ctgttttctg ctgtttaccg tgattgaaaa actaccgggg cgaaatgtta aaactgactg
    11041 atatcacctg gctttatcac catttgccga tgcgttttag cttaacggta gaacgcggcg
    11101 agcaggtggc aatcctcggg ccaagcggcg cgggtaaaag taccctgctg aatttgattg
    11161 ccggttttct gacgccagcc agcggttcgc tgactatcga tggtgttgat cacaccacga
    11221 cgccgccgtc acgccgtccg gtgtcgatgc tgtttcagga gaacaacctg ttcagccatc
    11281 tgacggtcgc gcagaacatc gggctggggc taaatccggg gttgaaactg aacgcggcac
    11341 agcaggagaa aatgcacgcg attgcccgtc agatggggat tgataattta atggcgcggt
    11401 taccgggcga gctttccggc ggtcagcgac agcgcgtggc gttagcgcgt tgtctggtgc
    11461 gcgaacagcc gattttattg ctcgatgaac cgttctctgc gctcgatccg gcgttacgtc
    11521 aggagatgct gacgctggtg agcacgagct gccagcagca aaaaatgacg ctattgatgg
    11581 tgtcgcacag tgtggaagat gcggcgcgga tcgccacacg ctcggtggtg gtcgccgacg
    11641 ggcgcatcgc ctggcagggt aagactgaag aattgttgag cggtaaggcg agtgcttcgg
    11701 caatattggg gattacgggt tagtatgcac tgtcagatgc ggcgtgaaca ccttatccga
    11761 cctacggaca gtgcgttttg taggcctgat aagacgcggt cagcgtcgca tgaggcaacc
    11821 cgcacaagac aatcccctaa accccaacca ctttacgcaa aatatcgata tacaccggca
    11881 tcaacgggtg gcgaattaac accaccagcg ccaccacgcc gatggcagaa atcaacggcg
    11941 tcagccacaa caaacggccg cgcgacaaat aatgacttaa acgatcggtt gctttaccgc
    12001 tgcgccataa ccgccagcac agccagccac caacccacaa aaataccgcc gtcgccagca
    12061 gcaaccattt aaactcgccg ctctgcattc cggctggaat atcgatcgcc gcgcccgcca
    12121 gaatcccagg caggaagtaa aacggtggcc acagcaggca gccgataata ttcggcgtaa
    12181 tgaattttgc caccggcaga tccagcattc ccgccaccat tggcaccagc ggacgcgtcg
    12241 ggccaacaaa gcgaccgacc agaatggtga acatgctgtg ttgatgcaac gcatgttcag
    12301 ttttatcgag taatgctttg tttttcttca gaaatgacca gcgatgcaac ggctttttaa
    12361 aacgccagcc cagccagaag gagatccagt cgcccagcag acaaccgaca atccctgcca
    12421 gccaagcgtg ccagaaactt aactcgccgc tgccaatcag cgctcccagc cccgccatca
    12481 gcaccgtacc gggtagaatc aaaccgacca gcgccagcga ctccagaaag gccaccaaca
    12541 ccaccgccat caatgaatac acggtagatt gggtaataaa gtgttccagc aatgcttgca
    12601 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag
    12661 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt
    12721 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
    12781 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
    12841 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
    12901 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
    12961 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
    13021 gcctcgcgta cacgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
    13081 cgcagtaaca attgctcaag cagattcatc gccagcagct ccgaatagcg cccttcccct
    13141 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat acggctggtg cgcttcatcc
    13201 gggcgaaaga aacccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
    13261 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacaaccg
    13321 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca gacaaattct
    13381 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
    13441 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
    13501 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
    13561 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga
    13621 cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt aaccccgctt
    13681 attaaaagca ttctgtaaca aagcaggacc aaagccatga caaaaacgcg taacaaaagt
    13741 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
    13801 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
    13861 tctactgttt ctccataccc gtttttttgg atggagtgaa acgatggcga ttgcaattgg
    13921 cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg gactgcgcca gcggtgaaga
    13981 gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa gggcaatttt gtgatgcccc
    14041 gaataaccag ttccgtcatc atccgcgtga ctacattgag tcaatggaag cggcactgaa
    14101 aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct gtggtcggga ttggcgttga
    14161 cagtaccggc tcgacgcccg caccgattga tgccgacggt aacgtgctgg cgctgcgccc
    14221 ggagtttgcc gaaaacccga acgcgatgtt cgtattgtgg aaagaccaca ctgcggttga
    14281 agaagcggaa gagattaccc gtttgtgcca cgcgccgggc aacgttgact actcccgcta
    14341 tattggcggt atttattcca gcgaatggtt ctgggcaaaa atcctgcatg tgactcgcca
    14401 ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact gggtgccagc
    14461 tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc ggacgttgca gcgccgggca
    14521 taaatctctg tggcacgaaa gctggggcgg cttgccgcca gccagtttct ttgatgagct
    14581 ggacccgatc ctcaatcgcc atttgccttc cccgctgttc actgacacct ggactgccga
    14641 tattccggtg ggcaccttat gcccggaatg ggcgcagcgt ctcggcctgc ctgaaagcgt
    14701 ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca gttggcgcag gcgcacagcc
    14761 taacgcactg gtaaaagtta tcggtacttc cacctgcgac attctgattg ccgacaaaca
    14821 gagcgttggc gagcgggcag ttaaaggtat ttgcggtcag gttgatggca gcgtggtgcc
    14881 tggatttatc ggtctggaag caggccaatc ggcgtttggt gatatctacg cctggtttgg
    14941 tcgcgtactc ggctggccgc tggaacagct tgccgcccag catccggaac tgaaagcgca
    15001 aatcaacgcc agccagaaac aactgcttcc ggcgctgacc gaagcatggg ccaaaaatcc
    15061 gtctctggat cacctgccgg tggtgctcga ctggtttaac ggtcgtcgca cgccaaacgc
    15121 taaccaacgc ctgaaagggg tgattaccga tcttaacctc gctaccgaca ctccgctgct
    15181 gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc gcaatcatgg agtgctttac
    15241 cgatcagggg atcgccgtca ataacgtgat ggcgctgggc ggcatcgcgc ggaaaaacca
    15301 ggtcattatg caggcctgct gcgacgtgct gaatcgcccg ctgcaaattg ttgcctctga
    15361 ccagtgctgt gcgctcggtg cggcgatttt tgctgccgtc gccgcgaaag tgcacgcaga
    15421 catcccatca gcccagcaaa aaatggccag tgcggtagag aaaaccctgc aaccgcgcag
    15481 cgaacaggca caacgctttg aacagcttta tcgccgctat cagcaatggg cgatgagcgc
    15541 cgaacaacac tatcttccaa cttccgcccc ggcacaggct gcccaggccg ttgcgactct
    15601 ataaggacac gataatggcg atttttgata attatgaagt gtggtttgtc attggcagcc
    15661 agcatctgta tggcccggaa accctgcgtc aggtcaccca acatgccgag cacgttgtta
    15721 atgcgctgaa tacggaagcg aaactgccct acaaactggt gttgaaaccg ctgggcacca
    15781 cgccggatga aatcaccgct atttgccgcg acgcgaatta cgacgatcgt tgcgctggtc
    15841 tggtggtgtg gctgcacacc ttctccccgg ccaaaatgtg gatcaacggc ctgaccatgc
    15901 tcaacaaacc gttgctgcaa ttccacaccc agttcaacgc ggcgctgccg tgggacagta
    15961 tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg cggtcgcgag ttcggcttca
    16021 ttggcgcgcg tatgcgtcag caacatgccg tcgttaccgg tcactggcag gataaacaag
    16081 cccatgagcg tatcggctcc tggatgcgtc aggcggtctc taaacaggat acccgtcatc
    16141 tgaaagtctg ccgatttggc gataacatgc gtgaagtggc ggtcaccgat ggcgataaag
    16201 ttgccgcaca gatcaagttc ggtttctccg tcaatacctg ggcggttggc tatctggtgc
    16261 aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct ggtcgatgag tacgaaagct
    16321 gctacaccat gacgcctgcc acacaaatcc acggcgaaaa acgacagaac gtgctggaag
    16381 cggcgcgtat tgagctgggg atgaagcgtt tcctggaaca aggtggcttc cacgcgttca
    16441 ccaccacctt tgaagatttg cacggtctga aacagcttcc aggtctgccc gtacagcgtc
    16501 tgatgcagca gggttacggc tttgcgggcg aaggcgactg gaaaaccgcc gccctgcttc
    16561 gcatcatgaa ggtgatgtca accggtctgc agggcggcac ctcctttatg gaggactaca
    16621 cctatcactt cgagaaaggt aatgacctgg tgctcggctc ccatatgctg gaagtctgcc
    16681 cgtcgattgc cgtagaagag aaaccgatcc tcgacgttca gcatctcggt attggtggta
    16741 aggacgatcc tgcccgactg atcttcaata cccaaaccgg tccagcgatt gtcgccagcc
    16801 tgattgatct cggcgatcgt taccgtctgc tggttaactg tatcgacacg gtgaaaacac
    16861 cgcactccct gccgaaactg ccggtggcga atgcactgtg gaaagcgcaa ccggatctgc
    16921 caactgcttc cgaagcgtgg atcctcgctg gtggcgcgca ccataccgtc ttcagccatg
    16981 cgctgaacct caacgatatg cgccagttcg ccgagatgca cgacattgaa atcacggtga
    17041 ttgataacga cacccgcctg ccagcgttta aagacgcact gcgctggaac gaagtgtatt
    17101 acggatttcg tcgctaagta gccacatcag gtatgtaacg cctgatgcga cgctgacgcg
    17161 tcttatctgg cctacacgct gcgattttgt aggccggata agcaaagcgc atccggcatt
    17221 caacgcctga tgcgacgctg gcgcgtctta tcaggcctac gcgctgcgat tttgtagacc
    17281 ggataagcga agcgcatccg gcacgaagga gtcaacatgt taaaagatct caaacgccag
    17341 gtattagaag ccaacctggc gctgccaaaa cacaacctgg tcacgctcac atggggcaac
    17401 gtcagcgccg ttgatcgcga gcgcggcgtc tttgtgatca aaccttccgg cgtcgattac
    17461 agcgtcatga ccgctgacga tatggtcgtg gttagcatcg aaaccggtga agtggttgaa
    17521 ggtacgaaaa agccctcctc cgacacgcca actcaccggc tgctctatca ggcattcccc
    17581 tccattggcg gcattgtgca tacgcactcg cgccacgcca ccatctgggc gcaggcgggt
    17641 cagtcgattc cagcaaccgg caccacccac gccgactatt tctacggcac cattccctgc
    17701 acccgcaaaa tgaccgacgc agaaatcaac ggcgaatatg agtgggaaac cggtaacgtc
    17761 atcgtagaaa cctttgaaaa acagggtatc gatgcagcgc aaatgcccgg cgttctggtc
    17821 cattcccacg gcccgtttgc atggggcaaa aatgccgaag atgcggtgca taacgccatc
    17881 gtgctggaag aggtcgctta tatggggata ttctgccgtc agttagcgcc gcagttaccg
    17941 gatatgcagc aaacgctgct gcataaacac tatctgcgta agcatggcgc gaaggcatat
    18001 tacgggcagt aatgactgta taaaaccaca gccaatcaaa cgaaaccagg ctatactcaa
    18061 gcctggtttt ttgatggatt ttcagcgtgg cgcaggcagg ttttatctta acccgacact
    18121 ggcgggacac cccgcaaggg acagaagtct ccttctggct ggcgacggac aacgggccgt
    18181 tgcaggttac gcttgcaccg caagagtccg tggcgtttat tcacgccgat caggttcccc
    18241 gcgctcagca tattttgcag ggtgaacaag gctttcgtct gacaccgctg gcgttaaagg
    18301 attttcaccg ccagccggtg tatggccttt actgtcgcgc ccatcgccaa ttgatgaatt
    18361 acgaaaagcg tctgcgtgaa ggtggcgtta ccgtctacga ggccgatgtg cgcccgccag
    18421 aacgctatct gatggagcgg tttatcacct caccggtgtg ggtcgagggt gatatgcaca
    18481 atggcactat cgttaatgcc cgtctgaaac cgcatcccga ctatcgtccg ccgctcaagt
    18541 gggtttctat agatattgaa accacccgcc acggtgagct gtactgcatc ggcctggaag
    18601 gctgcgggca gcgcatcgtt tatatgctgg ggccggagaa tggcgacgcc tcctcgcttg
    18661 atttcgaact ggaatacgtc gccagccgcc cgctgctgct ggaaaaactc aacgcctggt
    18721 ttgcaaacca cgatcccgat gtgatcatcg gctggaacgt ggtgcagttc gatctgcgaa
    18781 tgctgcaaaa acatgccgag cgttaccgtc ttccgctgcg tcttgggcgc gataatagcg
    18841 agctggagtg gcgcgagcac ggctttaaaa acggcgtctt ttttgcccag gctaaaggtc
    18901 ggctaattat cgacggtatc gaggcgctga aatccgcgtt ctggaatttc tcttcattct
    18961 cgctggaaac tgtcgctcag gagctattag gcgaaggaaa atctatcgat aacccgtggg
    19021 atcgaatgga cgaaattgac cgccgtttcg ccgaagataa acctgcgctg gcaacttata
    19081 acctgaaaga ttgcgagctg gtgacgcaga tcttccacaa aactgaaatc atgccatttt
    19141 tactcgaacg agcaacggtg aacggcctgc cggtggatcg gcacggcggt tcggtggcag
    19201 cgtttggtca tctctatttt ccgcgaatgc atcgcgctgg ttatgtcgcg cctaatctcg
    19261 gcgaagtgcc gccgcacgcc agccctggcg gctacgtgat ggattcacgg ccagggcttt
    19321 atgattcagt gctggtgctg gactataaaa gcctgtaccc gtcgatcatc cgcacctttc
    19381 tgattgatcc cgtcgggctg gttgaaggca tggcgcaacc tgatccagag cacagtaccg
    19441 aaggttttct cgatgcctgg ttctcacggg agaaacattg cctgccggag attgtgacta
    19501 acatctggca cgggcgcgat aaagccaaac gtcagggtaa taagccgctt tcgcaggcgc
    19561 tgaagatcat tatgaatgcc ttttatggcg tgctcggcac caccgcctgc cgcttcttcg
    19621 atccgcgcct ggcatcgtcg atcaccatgc gtggtcatca gatcatgcgg cagaccaaag
    19681 cattgattga agcgcagggc tacgacatta tctacggaga taccgactcg acttttgtct
    19741 ggctaaaagg tgcacattcg gaagaagagg cggcgaaaat cggtcgtgca ctggtgcagc
    19801 acgttaacgc ctggtgggcg gaaacgctgc aaaaacaacg gctgaccagc gcattagaac
    19861 tggagtatga aactcatttc tgccgttttc ttatgccaac cattcgcgga gctgataccg
    19921 gcagtaaaaa gcgctacgcc gggctgattc aggagggcga caagcagcgg atggtgttta
    19981 aagggctgga aaccgtgcgc accgactgga cgccgctggc ccagcagttt cagcaggagc
    20041 tgtacctgcg tatcttccgc aacgagccat atcaggagta cgtccgccaa accatcgaca
    20101 aactgatggc gggtgaactg gatgcgcggc tggtttaccg taaacgcctt cgccgtccgc
    20161 tgagcgaata tcagcgtaat gttccgcctc atgtacgcgc cgctcgcctt gccgatgaag
    20221 aaaatcaaaa gcgcggtcgc cccttgcaat atcagaatcg cggcaccatt aagtacgtat
    20281 ggaccaccaa cggcccggag ccgctggact accaacgttc accactggat tacgaacact
    20341 atctgacccg ccagctacaa cccgtggcgg agggaatact cccttttatt gaggataatt
    20401 ttgctacact tatgaccggg caacttgggc tattttgagc aaaaaaaaga gttcgccaga
    20461 taccattttg atgcgtgacg aatgctttgc catccagtac catagcgccc tttccattcc
    20521 tggacctgaa taacaccact acctcataag cacggtagcg ggtggttatt gcctgcaatt
    20581 aaagatatag agccgaacac atatgccttt tacacttggt caacgctgga tcagcgatac
    20641 agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat gcgcgaactg tcactttact
    20701 tttcccatct actggtgaaa accgtctgta cgcacgcagt gattcccccg tgacccgcgt
    20761 gatgttcaac cctggtgata ccattaccag ccatgacggc tggcagatgc aagtcgaaga
    20821 agtaaaagaa gaaaatggct tgctgaccta tatcggtact cgcctggata ctgaagagtc
    20881 cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg gtgttcagca aaccgcagga
    20941 ccgtctgttt gccgggcaga ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg
    21001 taagtattcc agcgaacagt tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag
    21061 cctgatcccg catcagctca acatcgctca tgatgttggt cgccgccacg cgccgcgcgt
    21121 cctgctggct gacgaagtgg gtttagggaa aaccattgaa gccgggatga tcctgcatca
    21181 gcaactgctc tctggcgctg ctgaacgtgt gctgattatc gtcccggaaa ccttacagca
    21241 tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc tttgctctgt ttgatgatga
    21301 gcgttatgcc gaagctcagc acgatgctta caacccgttt gacaccgaac agctggtgat
    21361 ttgctcgctg gattttgccc gtcgtagcaa acagcgcttg gaacatctct gtgaagccga
    21421 atgggacctg ctggtggtcg atgaagcgca tcacctggtg tggagcgaag atgcgccaag
    21481 ccgtgaatat caggccattg aacaactggc agagcacgtg ccgggcgttc tgctgctgac
    21541 cgcgaccccg gaacagctgg ggatggaaag ccacttcgcc cgtctgcgtc tgctggaccc
    21601 gaaccgtttc cacgattttg cgcagttcgt tgaagagcag aaaaattatc gtccggttgc
    21661 ggacgccgtt gccatgctgc tggcaggtaa caaactgagc aatgacgaac tgaacatgct
    21721 cggcgagatg atcggcgagc aggatatcga gccgctgttg caggcagcaa acagcgacag
    21781 cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg ctgatggatc gccacggcac
    21841 cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa ggattcccga aacgcgagct
    21901 gcacaccatt aagctgccgc taccgacgca gtatcagacg gctattaaag tctccggcat
    21961 tatgggcgca cgtaaaagtg cggaagatcg tgctcgcgat atgctctacc cggagcgtat
    22021 ttatcaggaa tttgaaggtg ataacgccac ctggtggaac ttcgatccgc gcgttgagtg
    22081 gctgatgggc tacctgacca gccatcgctc tcagaaagtg ctggtgatct gcgccaaagc
    22141 tgccactgcg ctgcaactgg agcaggtact gcgcgaacgt gaaggtattc gcgctgcggt
    22201 gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct gccgcctggt ttgccgaaga
    22261 agacaccggc gcacaggtac tgctgtgctc agaaatcggt tctgaaggac gtaactttca
    22321 gttcgccagt cacatggtga tgtttgacct gccattcaac ccggatctac tggagcagcg
    22381 tattggtcgt ctggatcgta tcggccaggc gcacgatatt cagatccatg tgccttatct
    22441 ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac gaaggtctgg atgcatttga
    22501 gcacacctgc ccgaccggac gcactattta cgatagcgta tacaacgatc tgattaacta
    22561 tctggcttca ccggatcaaa ccgaaggctt tgacgatctg atcaaaagct gccgcgagca
    22621 acatgaagcg ctgaaagcac agctggaaca gggtcgtgac cgcctgctgg aaatccactc
    22681 caacggtggc gaaaaagccc aggcactggc agaaagcatt gaagagcagg atgacgatac
    22741 caacctgatc gccttcgcca tgaacctgtt cgatattatc ggtatcaatc aggacgatcg
    22801 cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg ctggtgccgg acttccctgg
    22861 cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa gtggcgctgg cgcgtgaaga
    22921 tgcacagttt attacctggg agcatccgct gatccgcaac ggtctggatc tgatcctttc
    22981 tggcgatacc ggtagcagca cgatttcact gttaaaaaac aaagcgttgc cggtaggtac
    23041 gctgttggtg gaactgattt atgtggttga agcccaggct ccgaagcagt tgcagctcaa
    23101 ccgcttcctg ccaccgacgc cggtacgtat gctgctggat aaaaacggca acaacctggc
    23161 ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac gcggttaacc gtcacaccgg
    23221 cagcaaactg gttaacgccg tgcagcagga tgttcacgct atccttcaac tgggtgaagc
    23281 gcagatcgag aaatctgccc gtgcattgat tgatgcagcg cgtaacgaag ccgacgaaaa
    23341 actgtctgcc gagctgtctc gtctggaagc tctgcgtgca gtgaacccga acattcgtga
    23401 cgacgaactg accgccattg agagcaaccg tcagcaggta atggaaagcc tggatcaggc
    23461 aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg catcagtaac ggagccgaaa
    23521 atggggatgg aaaactacaa tccaccgcag gaaccctggt tggttatcct gtatcaggat
    23581 gaccatatta tggtggtcaa caagccgagc ggtttgttgt cagtgccggg tcgtctggaa
    23641 gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt atccgcaggc agaatcggtg
    23701 catcgtctgg atatggctac cagcggcgtg attgtagtgg cgctgaccaa agccgcggag
    23761 cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa agcagtatgt ggcccgcgtc
    23821 tgggggcatc catcccccgc agaaggtctg gtggatctgc cgctgatttg cgactggcca
    23881 aaccgcccga aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac ggaatatgaa
    23941 gtggtggagt atgcggcgga taacacggca agagtggtgt taaaaccgat taccgggcgt
    24001 tcgcatcaat tgcgtgtgca tatgctggcg ctggggcatc cgattctcgg cgatcgtttt
    24061 tatgcatcac cagaagcgag agcgatggca ccgcgtttgt tgctacatgc agagatgttg
    24121 acgattaccc atccggcgta tggcaatagt atgacgttta aagcgccagc ggatttttaa
    24181 tcgtcacgct ggcgcagttc ggtaggcgga taaggcgttc acgccgcatc cggcaattgt
    24241 gcgccgattg cctgatgcga cgcgggccgc gtcttatcag gcctacagtt taaaacttat
    24301 ttaaaccctt tctgctgctt tatcagctca tatgcctgct gaatttcctg cgctttctgc
    24361 ttcgccatct ccatcatctc aggcggcaaa cctttcgcca ccagcttatc gggatggtgt
    24421 tcactcatca gcttacggta ggcacgtttg atggtggtcg catcatccgt cggcttcacg
    24481 cccagcacat tacaggcatc ttccagcgtc gggccacgct gcgcttgctg ccagttacca
    24541 ccgccagatt gctgctgata accgccgcca aactgtgcac cgccctgcat catgcgcaaa
    24601 aactggtcaa actgggcgcg ggagatccct aattcttcag caatgacata cagcaccgcc
    24661 cgttcattcg ggtgcagtga accatcagca aacgccgcct gaatctggat ctccagaaac
    24721 atacgaatta agtcaaaacg accaaagcag acactgcgaa actggcgcat cttttcgcgc
    24781 agcgggtaat tatctgattt tcccacccgg aacgcgtttt gcgccgcagt acgggaagcg
    24841 ccatgaaggt tcattcggtc catcaactgg ctggcgatat gaatatcagc ctccgtgacg
    24901 cgacctttgg atttggttaa atgccccatc acttcaaaag tggtggcaaa aaacagcgcc
    24961 tgacgctcac gctggttggc gaaccacgcc attttacggc tacgggcttt atcaaacata
    25021 tggccaatta acaggcctaa cactacgccc caaaagccgc cgcccatcag taaggccacg
    25081 gccacgccaa tgatttttcc ccaatactgc atatattccc caaatcgaca cacggatatc
    25141 agggctatct cccacaatat aaaggtgctt ttaccgtttt ccggctgcgg tcaattgtgg
    25201 gacatcgcct ataatttgca ttatcatacc tgtcattcaa tgccgtgcct aacaccactg
    25261 acgctattcg gacaggatta acactagcgt agagatgacg agtacgttag tctctgaccg
    25321 tttgtcacgc gcaacgttac cgatgatgga acaataaaat caacgtatga aaaaacgtat
    25381 ccccactctc ctggccacca tgattgccac cgccctttat agtcaacagg gactggcagc
    25441 cgacctcgcc tcacagtgca tgttgggcgt gccaagctat gaccgtcctc tggtacaggg
    25501 cgataccaat gacttacccg tgactatcaa tgctgaccac gcgaaagggg actacccgga
    25561 tgacgccgtg tttactggca gcgtggatat catgcagggt aacagccgtc tgcaggccga
    25621 cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg gagccggtac gtaccgttga
    25681 tgcgctcggt aatgtccatt acgacgataa ccaggtgatc ctcaaagggc cgaaaggctg
    25741 ggcgaatctg aacaccaaag ataccaacgt ctgggaaggt gattaccaga tggtgggtcg
    25801 ccagggtcgc ggtaaagcgg acctgatgaa acaacgtggc gaaaaccgct ataccattct
    25861 ggataacggt agctttacct cctgtctgcc gggttctgac acctggagcg tggtaggtag
    25921 cgaaattatt catgaccgcg aagaacaagt tgctgagatc tggaacgccc gctttaaggt
    25981 gggtccggta ccgatctttt atagccccta tttgcagttg ccggtgggtg acaaacgtcg
    26041 ctctggtttc ttgatcccga acgccaagta caccaccacc aactactttg agttctacct
    26101 gccatattac tggaacatcg cgccaaatat ggatgccacc atcacgccgc attatatgca
    26161 tcgtcgtggc aacatcatgt gggagaacga attccgctac ctctcccagg cgggcgctgg
    26221 cttgatggaa ctggactatc tgccttcaga taaagtctat gaagatgaac acccgaacga
    26281 tgacagttca cgtcgttggt tattctactg gaaccactcc ggggtcatgg atcaggtgtg
    26341 gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc tacttcaatg atttcgataa
    26401 caagtacggt tccagtactg acggctacgc aacgcaaaaa ttcagcgttg gctatgcggt
    26461 gcaaaacttc aatgccaccg tttcaaccaa gcagttccag gttttcagcg aacagaacac
    26521 cagtagctac tcggcagagc cgcagttaga cgttaattac taccagaatg atgttggtcc
    26581 gtttgatacg cgtatttacg gccaggcagt gcactttgtt aacaccagag acgacatgcc
    26641 tgaagcaacc cgtgttcacc tggaaccgac catcaatttg ccgctctcta ataactgggg
    26701 cagcatcaat accgaagcga agtttctggc aacccattat cagcaaacca atcttgactg
    26761 gtataactcc agaaacacga ccaagctgga cgaatccgtt aaccgcgtaa tgccgcaatt
    26821 caaagttgac ggcaaaatgg tctttgaacg cgatatggaa atgctggctc cgggttatac
    26881 ccaaacgctg gaaccgcgcg cgcagtattt gtacgtgccg tatcgcgatc agagcgacat
    26941 ctataactac gactcgtctc tgctgcaatc tgactactct ggcctgttcc gggaccggac
    27001 ttacggcggt cttgaccgta ttgcctccgc taaccaagtg acgaccggtg tcacatctcg
    27061 catatatgat gatgctgccg ttgaacgttt taatatttcc gttggtcaaa tctactattt
    27121 cacggagtct cgcactggcg atgacaacat aacatgggag aatgacgaca aaacgggctc
    27181 actggtgtgg gcaggcgaca cctactggcg tatctccgag cgttggggat tgcgtggcgg
    27241 gattcagtac gatacacgtc tggataacgt agcgaccagt aactccagca ttgaataccg
    27301 tcgggatgaa gaccgtctgg tacagctgaa ttaccattac gccagcccgg aatatattca
    27361 ggctacgctg cctaagtact attccactgc tgagcaatat aagaatggta tttcgcaggt
    27421 aggtgctgtc gccagcaggc caattgccga tcgttggtcc attgttgggg cctactacta
    27481 cgacaccaat gcgaacaagc aagccgactc tatgttaggt gtgcaataca gctcctgctg
    27541 ctatgcaatt cgcgtcggtt acgagcggaa gctgaacggt tgggataacg ataaacaaca
    27601 tgcggtatat gacaacgcaa tcggctttaa catcgaactt cgcggcctga gctccaacta
    27661 cggtctgggt acgcaagaga tgctgcgttc gaacattctg ccgtatcaaa acactttgtg
    27721 atttgttgat ttaccacgta atccgcagtg cggttaattg aaatggaaaa agtatgaaga
    27781 actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa taccagtttc gctgcccccc
    27841 aggtagtcga taaagtcgca gccgtcgtca ataacggcgt cgtgctggaa agcgacgttg
    27901 atggattaat gcagtcggta aaactgaacg ctgcgcaggc aaggcagcag cttcctgatg
    27961 acgcgacgct gcgccaccaa atcatggaac gtttgatcat ggatcaaatc atcctgcaga
    28021 tggggcagaa aatgggagtg aaaatctccg atgagcagct ggatcaggcg attgctaaca
    28081 tcgcgaaaca gaacaacatg acgctggatc agatgcgcag ccgtctggct tacgatggtc
    28141 tgaactacaa cacttatcgt aaccagatcc gcaaagagat gattatctct gaagtgcgta
    28201 acaacgaggt gcgtcgtcgc atcaccatcc tgccacagga agtcgaatcc ctggcgcagc
    28261 aggtgggtaa ccaaaacgac gccagcactg agctgaacct gagccacatc ctgatcccgc
    28321 tgccggaaaa cccgacctct gatcaggtga acgaagcgga aagccaggcg cgcgccattg
    28381 tcgatcaggc gcgtaacggc gctgatttcg gtaagctggc gattgctcat tctgccgacc
    28441 agcaggcgct gaacggcggc cagatggggt ggggccgtat tcaggagttg ccggggatct
    28501 tcgcccaggc attaagcacc gcgaagaaag gcgacattgt tggcccgatt cgttccggcg
    28561 ttggcttcca tattctgaaa gttaacgacc tgcgcggcga aagcaaaaat atctcggtga
    28621 ccgaagttca tgctcgccat attctgctga aaccgtcgcc gatcatgact gacgaacagg
    28681 cccgcgtgaa actggagcaa attgctgctg atatcaagag tggtaaaacg acttttgctg
    28741 ccgcagcgaa agagttctcg caggatccag gttctgctaa ccagggcggt gatctcggct
    28801 gggctacacc agatattttc gatccagcct tccgtgacgc cctgacccgc ctgaacaaag
    28861 gtcaaatgag tgcaccggtt cactcttcat tcggctggca tttaatcgaa ctgctggata
    28921 cccgtaatgt cgataaaacc gacgctgcgc agaaagatcg tgcataccgc atgctgatga
    28981 accgtaagtt ctcggaagaa gcagcaagct ggatgcagga acaacgtgcc agcgcctacg
    29041 ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt gtgatcactc ccggcgagcc
    29101 cgccgggatt ggcccggact tagttgtcca gcttgcacag cgtgagtggc cggtcgaact
    29161 ggttgtttgt gccgatgcca ctctccttac cgaccgggca gcgatgctcg gtttgccgct
    29221 caccctccgc ccttattccc ccaactcccc tgcacaaccg caaactacgg gcacattaac
    29281 gctacttcct gtcgcgctac gtgaatctgt cactgcgggg cagttagcga ttgaaaatgg
    29341 gcattacgtg gtggagacgc tggcgcgagc gtgtgatggc tgtctgaacg gtgaatttgc
    29401 tgcgctgatc acaggccccg tgcataaagg cgtcattaac gacgcaagca ttccgtttac
    29461 cggtcatacc gagtttttcg aagagcgttc gcaggcgaaa aaagtggtga tgatgctggc
    29521 gaccgaagaa cttcgcgtgg cgctggcaac gacgcattta ccgctgcgcg atatcgcaga
    29581 tgctatcacc cctgcacttt tgcacgaagt gattgctatt ttgcatcacg atttgcgtac
    29641 aaaatttggt attgccgaac cgcgcattct ggtctgcggg ctgaatccgc acgcgggcga
    29701 aggcggtcat atgggtacgg aagagataga caccattatt ccggtgctcg acgagctgcg
    29761 ggcgcagggg atgaaactca acgggccgct gcctgccgat accctgtttc agccgaaata
    29821 tctcgataac gccgacgccg tgctggcgat gtaccacgat cagggtcttc ccgtgctaaa
    29881 ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc ctgcccttta ttcgcacatc
    29941 agtggaccac ggcaccgcgc ttgaactggc gggacgtggc gaagccgatg tcggcagttt
    30001 tattacggcg cttaatctcg ccatcaaaat gattgttaac acccaatgaa taatcgagtc
    30061 caccagggcc acttagcccg taaacgcttc gggcaaaact ttctcaacga tcagttcgtg
    30121 atcgacagca ttgtttctgc cattaacccg caaaagggcc aggcaatggt cgaaatcggc
    30181 cccggtctgg cggcattgac cgagccggtc ggcgaacgtc tggaccagct gacggtcatc
    30241 gaacttgacc gcgatctgac ggcacgtctg caaacgcatc cattcttagg cccgaaactg
    30301 acgatttatc agcaggatgc gatgaccttt aactttggcg aactggccga gaaaatgggt
    30361 cagccgctgc gtgttttcgg caacctgcct tataacatct ccacgccgtt gatgttccat
    30421 ctgtttagct atactgatgc cattgccgac atgcacttta tgttgcaaaa agaggtggtg
    30481 aatcgtctgg ttgcaggacc gaacagcaaa gcgtatggtc gattaagcgt catggcgcaa
    30541 tactattgca acgtgatccc ggtactggaa gttccgccgt cagcctttac accaccaccc
    30601 aaagtggatt ccgccgtcgt gcgcctggtc cctcatgcaa cgatgcctca cccggttaaa
    30661 gatattcgcg tgttgagccg catcaccacc gaagccttta accagcgtcg taaaaccatt
    30721 cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa ccggaatggg gatcgacccg
    30781 gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc agatggcgaa ctatctggcg
    30841 gagaacgcgc ctttgcagga gagttaacga tgatcaattc gccccgagtg tgtattcagg
    30901 ttcaaagcgt ctacattgag gcacaatctt cacctgataa tgaacgttac gtttttgctt
    30961 ataccgtaac catacgcaat ctggggcgag cgccagtgca gttgttgggg cgttactggc
    31021 tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg cgaaggagtg gttggcgtcc
    31081 agccacttat cgcgcctggc gaagagtacc agtacaccag cggtgcaatc attgaaaccc
    31141 cgctgggcac catgcagggt cactacgaaa tgatcgatga aaatggcgtc cctttcagca
    31201 tcgacattcc cgtattccga ctcgccgttc ccacactcat tcattaaaag aatatggcga
    31261 cataccttat tggcgacgtt catggttgtt acgatgaact gatcgcattg ctgcataaag
    31321 tagaatttac ccccgggaaa gataccctct ggctgacggg cgatctggtc gcgcgcggcc
    31381 cgggttcgct ggatgttctg cgctatgtga aatccttagg cgacagcgta cgtctggtgc
    31441 tgggcaatca cgatctgcat ctgctggcgg tatttgccgg gatcagccgc aataaaccga
    31501 aagatcgcct gacaccgctg ctggaagcgc cggatgccga cgagctgctt aactggctgc
    31561 gtcgccagcc tctgctgcaa atcgacgaag agaaaaagct ggtgatggcc cacgcaggga
    31621 tcacgccgca gtgggatctg cagaccgcca aagagtgcgc gcgcgatgta gaagcggtgc
    31681 tgtcgagtga ctcctatccc ttctttcttg acgctatgta cggcgatatg ccaaataact
    31741 ggtcaccgga attgcggggg ctgggaagac tgcgttttat caccaacgcc tttacccgta
    31801 tgcgtttttg cttcccgaac ggtcaactgg atatgtacag caaagaatcg ccggaagaag
    31861 cccctgcccc actgaaaccg tggtttgcga ttcctggccc tgtcgctgaa gaatacagca
    31921 tcgcctttgg tcactgggca tcgctggaag gcaaaggtac gccggaaggt atttacgcgc
    31981 tggataccgg ctgctgctgg ggtgggtcat taacctgcct gcgctgggaa gataaacagt
    32041 attttgtcca gccgtcgaac cggcataagg atttgggcga agcggcggcg tcttaaacac
    32101 agcctgatac aggaaggccg gataagacgc gaccggcgtc gcatccggca ctagccgtaa
    32161 attctataca aacttaccgc cgctccagaa tctcaaagca atagctgtga gagttctgcg
    32221 catcggcgtc gtggaattcg ctgaataccg attcccagtc atccggctcg taatccggga
    32281 aatgggtgtc gccttccact tctgcgtcga tatgcgtcag atacagtttt tgcgcttttg
    32341 gcaggaactg ctcataaacg cgaccgccgc caatcaccat gatttctggt acgtcaccac
    32401 acgccgcaat ggcttcatcc accgatttca cccacgttac gcgatcgtcc gtacccggtt
    32461 gactgctgag gataatattt ttgcgtcctg gcaacggacg accgattgat tcccaagtat
    32521 ggcggcccat aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg agatcggcag
    32581 gcaggttcca cggcatggcg ttttccatgc cgataacgcg atctaccgct aacgccgcaa
    32641 tcagactgat cattgagatt tcccgataaa aaaattgtcg ccactatacg taaagcgtaa
    32701 accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc
    32761 cacgctctcc ccctggactc gccgcattac aatgaaacaa aaacaaacag ttagctgtaa
    32821 agtgtgattt acgtcactct ttattaagat gagggtttcg tttccggttc atccgccatg
    32881 ttgccggtat gtttaccttc ttctgttccc tgccagccat gtcgttgaat taatgacaga
    32941 tgttcgcggt cctcggtgat gatctcactt aacatcgcgc tggtgcgttt atagaccgcc
    33001 gcgcgggctt tggtgtcatt ctcaaccatt gccatctctt ccaccatctg aatattaaag
    33061 cggcggaaca catcggcacg ttctcgcgct tcatacggcc ccagacctaa actttccagc
    33121 gccagacgcc cggttttcag cgcaccttca aaggtttcac gctccggctt ttcaacgcct
    33181 gcctgacgca aacgaatgta gtgatcgaca tcgcgggcgc gggcaataat ctgcaaatgc
    33241 gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg tttgcggatc gtcaatagcg
    33301 ttaatcagca cttccgcttt cgccgctccg gcagattcca gtaaatccat ccgcgtggca
    33361 tcgccataaa acactttcat accaaattta cgcagcgttt cgatatggtc cggatcgtga
    33421 tcgagcacca ccattttcac cccgctggag agcagtaaac gtccggtaat ctgtccaaaa
    33481 cgaccgaatc cggcgataat cacgcgcggc tgttcttcat cgatctcatc ggcttcacgc
    33541 gcctcctccg tagaagattg ctcaaggcga ttgaggatca ccagcagaat cggcgttgct
    33601 gccatcgaca gcgccaccgc cagggtcagc gatttcaccc actccggctc cagcacattc
    33661 gccatctgcg ccgcaccaaa taccacaaag gcaaactcac tgccctgccc taacaacacc
    33721 gcaaaccagc gacgctgttt atttggcacc tgcaacggtc gggcaatcag ccacagcatg
    33781 gcgattttga tgatgaggaa accgagcagc aaaatgacaa tgcgcaatgg gttttcaatc
    33841 agcgtgccaa agtctatcga catgccaaca ccgatgaaaa acagccccaa cagcaaacct
    33901 ttaaacggtt cgatatcgct ttccaacgca tgacggtatt ccgagcttgc cagcagtacg
    33961 cccgccagaa acgcgcccat cgccatcgac aagccgacct cttccagcag caaaccaaag
    34021 ccaaacacaa ggaataacgc cacggcacta aacacttccc gcaagccaga gcgggcaaca
    34081 aaacgcagcg ccggacgcgt aacatagcgc cccagcaata ccaccagtac cagcgcaccc
    34141 gccactttta acgccgagag agcaaatgcg cccatcgtcg tcgaggcact gctcgccgcc
    34201 agtagcggga tcatcgccac cagcgggatt gccgcgatat cctggaacag cagcactgca
    34261 aaggcactgc ggcccatttg cgtcaccatc agattgcgtt cattcatcgc ctgcatggca
    34321 atcgccgttg atgagagcgc cagcgtcata ccgatcaatt ccgcgacctg ccagcgcagc
    34381 ccaagtaaca tacagaacag ccccagcagg ccaccgcaaa tcaccatctg caatgcgcca
    34441 ccgccgaaca ctgccgcacg cagcttccac agcctttgtg gatcgagttc gaggccgata
    34501 ataaacagca tcagcaccac cccaatctcg gcaaagtgca gaatagattc ggcatcggtc
    34561 accagtcgca gcccccacgg gccaataatg cagccggcga tcaggtagcc aagtaccgat
    34621 cccagaccaa gacgtaccgc aatgggtaca atcagcgccg ccgatccgag gtagatcagc
    34681 gcctgaacca gcgtatgact atccatgatg ggcctcctgc cattccagca gacgttgctt
    34741 atagtggcgc gcctgccctt cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc
    34801 aaacggtggc agccagttca gcccgcagta gattgccgtc gcctgtagcg gctgcgacag
    34861 cacatcaaag cccggatgcg caccaatttc aaaatggctt tccccgccgc cggtcgtcac
    34921 cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg tgaccgtaag cccagccgtg
    34981 cgacaaaact ttatcgatcc aaagtttaag aagcggagga atgctgtacc actgcatcgg
    35041 gtgctgccag acgatcagat cggcgcgaga gagcgcctcc tgctcggcgg caatatcgat
    35101 attgaagtca ggatagagtt gataaagaga gcgaatttcg acgccttcca gcgtccttgc
    35161 ctgttcaagc atccgtttat tcgcatggga atgatgcgga tacggatgcg cataaatgat
    35221 gaggatcata gttattagcc cgcgtgttta cgcctttgtt ttcccacaaa gtgtagtcat
    35281 atattcacgc ggcttatagt caataatatt gattcagaaa agagagaaat ctgaacaaat
    35341 aatcccattc tctgcccgcg gatcgtcata ttgctggtct gcgccagtga cattcctcga
    35401 gtctccgggg caaacgctac ggaaatcaac aagccaaaca gcgagatacc cgcccccatt
    35461 agcatcgtgt tactgatacc gtaattattg ataaatatcg gcagtgccca ggtcgaaaca
    35521 atggtgccaa tacgacttaa ggacataatc acgcccacgg cagaggcgcg gatatccgtc
    35581 gggaagagtt cattaggata gagccactgc aaattacccg gcccgccaga gaaaaaggca
    35641 tacaccgcaa aggccatcac taccagccag atccccatat ccgggattag ccccaaaacc
    35701 gccaacgcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg tccggcagtg
    35761 tttagccaca gcatcggcgg aatacagccg agcataaaga acaggctaat caccacattc
    35821 cctagtgccg cgtttttgcc aacccccaat cccaacgaac caacgatttg cgggccaaag
    35881 gtgtaaatgg cgaacattgg gatcacctgg caggtccaga tggcggcaac aaacagaaca
    35941 aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg ctgctcttca
    36001 tcgaaagcca ccggttcgcc aaacagtttt atcatcatct cttcgcactc ttttactcgc
    36061 cctttgcgta ataaccagcg gggagattct ggcagttcga atcgaccaat caaaatcaac
    36121 aaacaaggga tcgccgcgct acccagcatc cagcgccaac caccttccac atcataaagc
    36181 cagtagccga ccagatcggc gcaggtcgcg ccgacatacc acatcgcggc aataaagctg
    36241 atggaaaacg cccgctgacg ggtactggag aactcggtga tcatcgaagt ggcgatggga
    36301 taatctgcac cgatgacaat gccgatgagt acccgcatca ccaacagttc gacgggggat
    36361 gaaacacaca tcgtcgccac cgatatcacg ccgatggcga tgatatcaat gaggaacatt
    36421 ttgcgccgtc cgactttatc ggagatatag ccaaacagcg atgtgccaac gaacagcccg
    36481 gcgagcgttc ccgcgcccag caagccaatc cagtcagcgt ccagtttcag cgccggagtc
    36541 agttgctcca gcgccacgcc aatcattacc agtacataac catccagaaa cggaccaccg
    36601 cttccccaca gcaaaatgcg gcggtgaata gaggagaatt tgagatcgtc aaagtttctg
    36661 gacggttgca tgggttattc ctgttttttt gcaatgagag tcagtacggc gttacgtatc
    36721 aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt attcccactg
    36781 ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac atccggcgta
    36841 atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc acgctttcac
    36901 cagcagctcc agcacctgtt tatcagcatc ggccttcaca acaatgtgcg gatgctcttc
    36961 atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag aagtcataaa
    37021 acggtcactc ctttcatgcc atccttgatc agattgatga agcccacttt cttgccgtgg
    37081 cggaggattt tcttgcgcat cagttccggc gcactgccgt caatggtgaa caggtcacgc
    37141 gccacgccca ccgccagttc cgggttgccg ctaaacatgc gtgggttatc aaggaacgcc
    37201 ggtagtttct ggtacatgcg catatcgcgc agcgggccac tctccagatg ctgacgatat
    37261 tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac ggtttttgct
    37321 gcagcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc gaggttcata
    37381 cacattccgg cggcatcacc ggcaatcaat acgccgtcac cgaccagctc cggcagcatg
    37441 ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc gcccgcgatc
    37501 agcggtgcaa cggccggatg ctgtttgaaa tcttccagca tttgcggcac cgattttttc
    37561 gcgtcatgca gatgatgcag accacaaacc agccccagcg acagggtatt ttcattggta
    37621 taaaggaagc cgccgcccat caggccatcg gtgggtgatc ccgcaaacag acaagccgcg
    37681 ccctgattac cctgcaactg aaaacggtct tcgattaccg acttcggtaa ctcgatcagt
    37741 tccttcacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc caacttttcg
    37801 gcaaggatgg agttcacccc atcagcaagg agcaccgttt tcgcttcaat cacatcacca
    37861 tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac gcggatcccg
    37921 gtaattaact gcgcgcccgc ttcttcggcc tgctccatca accaggcatc aaatttactg
    37981 cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt caccgttgca gtagtccata
    38041 gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag gcgttctacg
    38101 ggggcggaat cggcgaaacc tggaataatg tgttccagac tgtgggcata gagacgcccg
    38161 ccagtaacgt tcttggcacc tgcggaattg ccacgctcga taactaacac ttgcgcccct
    38221 tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc ctgcaccgac gatgatggcg
    38281 tcaaagatat cttcggacat aagagctccc tgtcagcggc agagccgtaa tgcagccctg
    38341 ccagagtgga tcaacgcgct aaagctgcgg tcagtgccgg aaggatcttc acggcatcgc
    38401 caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatcttta ttgatggcga
    38461 aaatggtttg cgacgcgtta gcgccaacca tgtgctggat ctacccggag atccccaccg
    38521 ccaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt tcgtgctcca
    38581 tccatttttc gttttctgcc accggacgag aacaggccaa ctccgcacct atcgccttgc
    38641 aaagctgttc tgccagcgca atgttctctt tgctgccaat gccgcgaccg acgctgacca
    38701 ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt gccgtgcggg
    38761 taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt
    38821 ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag taccgcatac ggcgtggcaa
    38881 tgcgttcttc gccaatcgcc agaccgccgt aaaccatgtg tttcactgtt gctttaccgt
    38941 cctgtacgct gacggtgctg gcatcgttag acaccgccgc tttaaggcga taacccagtt
    39001 ttgccgccag taatttgccg cgacgggtgt ttggcaacag caccagaccg tcagcgccgt
    39061 gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc gatcatccga tcgtccggtt
    39121 tgccgtttaa tttccagaca tgattagcgc cgagctggat tgcctgtgcg ccgtcggcat
    39181 cattgaggac aaaggcattg atttgattag ctaaagcctg cgcaccgttc atcagttccg
    39241 gcagacgaga gggggtatcg ctgaataccc agacttgaga aaacgtgttc atagcatccc
    39301 ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc
    39361 gccttcaatc acgatgcgct gacgctcgcg ctgtttcggc gcggcaacct gttgttcaga
    39421 ccaggcgtcc acggcgttaa aaccaatatc cgccgccgac catacctgga ctggcttttt
    39481 cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat cagtggaaac
    39541 agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt tcatcttcca gttcgcgctc
    39601 aacggtgagg gtatctgccg tcagggagat aattttgctg acgccgttaa ctgccggaat
    39661 attgaggatt tcgcccacca gcagaccaac ctgctgggca taaaggtcgg aagaaccatc
    39721 gccacagagg atcagatcaa agcctgcttt ctgggcggct gcagccagtg cgctcgccgt
    39781 ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt catccgggcc
    39841 gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc ccacacttaa
    39901 ggctgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt caatagcgtt
    39961 gagatcgtat tggcttattt tggcatcggc tttgctgaag tctaatgaac catcagcatt
    40021 attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa tcttcattgc
    40081 atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta cagatatcac
    40141 gccctttaat ttggaaataa aactttaatc a
//
LOCUS       DAZOZX010000024        39978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZOZX010000024 DAZOZX010000000
VERSION     DAZOZX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.024"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..155
                     /locus_tag="ACHUPT_001212"
     CDS             <1..155
                     /locus_tag="ACHUPT_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0818864.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHUPT_001213"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHUPT_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0818865.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHUPT_001214"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHUPT_001214"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0818866.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHUPT_001215"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHUPT_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHUPT_001216"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHUPT_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0818867.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHUPT_001217"
     CDS             complement(5626..7134)
                     /locus_tag="ACHUPT_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0818868.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHUPT_001218"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHUPT_001218"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0818869.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHUPT_001219"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHUPT_001219"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0818870.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHUPT_001220"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHUPT_001220"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0818871.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHUPT_001221"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHUPT_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0818872.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHUPT_001222"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHUPT_001222"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0818873.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHUPT_001223"
     CDS             complement(14283..15602)
                     /locus_tag="ACHUPT_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR0818874.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHUPT_001224"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHUPT_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0818875.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHUPT_001225"
     CDS             16699..17034
                     /locus_tag="ACHUPT_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0818876.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHUPT_001226"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHUPT_001226"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0818877.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHUPT_001227"
     CDS             complement(17790..19172)
                     /locus_tag="ACHUPT_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0818878.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHUPT_001228"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHUPT_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0818879.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHUPT_001229"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHUPT_001229"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0818880.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHUPT_001230"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHUPT_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0818881.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHUPT_001231"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHUPT_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0818882.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHUPT_001232"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHUPT_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0818883.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHUPT_001233"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHUPT_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0818884.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHUPT_001234"
     CDS             complement(25947..26768)
                     /locus_tag="ACHUPT_001234"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0818885.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHUPT_001235"
     CDS             complement(26868..26951)
                     /locus_tag="ACHUPT_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818886.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHUPT_001236"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHUPT_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0818887.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHUPT_001237"
     CDS             27623..27727
                     /locus_tag="ACHUPT_001237"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0818888.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHUPT_001238"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHUPT_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30038
                     /locus_tag="ACHUPT_001239"
     CDS             29136..30038
                     /locus_tag="ACHUPT_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0818889.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="ACHUPT_001240"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="ACHUPT_001240"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0818890.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="ACHUPT_001241"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="ACHUPT_001241"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0818891.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHUPT_001242"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHUPT_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR0818892.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHUPT_001243"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHUPT_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0818893.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHUPT_001244"
     CDS             complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHUPT_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0818894.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHUPT_001245"
     CDS             complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHUPT_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0818895.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="ACHUPT_001246"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="ACHUPT_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37857..38891)
                     /locus_tag="ACHUPT_001247"
                     /pseudo
     CDS             complement(37857..38891)
                     /locus_tag="ACHUPT_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            38949..>39978
                     /locus_tag="ACHUPT_001248"
     CDS             38949..>39978
                     /locus_tag="ACHUPT_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0818896.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10163 a         9696 c        10237 g         9882 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctag
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagtga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgcctagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgt gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct tccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcca ttgccagacg gatggatgga cttgaagtag ttgactcaat gctacttaag
    38941 gtcctgatat gactgacatt aatcatgata acactgtata tccatccagt tctttctctg
    39001 ggtctcccta tcggtatcag caagtctaca cctcaagccc tgacacaagg ctgacacaaa
    39061 tcaatctact cagcgtagct aagcctcagt tagtccgcag agccaatggt cgatatacta
    39121 tccgcttcag actcaaggga cagaccacac cgtttctttc agtatcgacc cgcagcacag
    39181 acaggagagt agccacaatg cgtcagagag agcttgcagc caccgctaag gccttcatgt
    39241 tggataggcc tgaagtctca ctacaggagc ttacagagca tctccggtct atggctgagc
    39301 agttcctgac agacgctagt gacgattact ggaatggact tgaggtcgct actctggtcg
    39361 acgagaagtc caacctgaag gagctagccg ctacgcaagc cttgagtctg gaccagcaga
    39421 agggcataag gttagcgctg gaagtcctga ccgcagcaca gcagagagtc gacactggtg
    39481 atacctctgg tctgattaag ctgattgatg ataataacct tactgatgac tcaactatag
    39541 gcgactctac gtcaattctg aataatgagc agggggatag accggcagtt ttcacacagg
    39601 agcgccagtc atctgtagtt ttttcaagcc tagtgtcctc gctgctggct gagaaggtcc
    39661 agaccctgaa gacatccagc tacaaggacc tctcgtcctc actcaatact gtctcccggt
    39721 tcctgcctga agacatggac ctgatgtccc gctctgggtg gctggctgtc agggactcta
    39781 tgctggcttc tgaggtgaga ccatcgacaa tcaacaagct gctgaccaaa gcgaagatgt
    39841 gtctcgatta tggcctgatg aatgggcaac ttgagggccg caaccctatt gaacggatga
    39901 agctgaccaa ggatatcgac tccaagcgca gagcgttcac agacgaggag ctggagagac
    39961 ttctggtccg tgttgagt
//
LOCUS       DAZOZX010000025        37423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZOZX010000025 DAZOZX010000000
VERSION     DAZOZX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.025"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1101)
                     /locus_tag="ACHUPT_001249"
     CDS             complement(<1..1101)
                     /locus_tag="ACHUPT_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0818897.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUPT_001250"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUPT_001250"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0818898.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUPT_001251"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUPT_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0818899.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUPT_001252"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUPT_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0818900.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUPT_001253"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUPT_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0818901.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUPT_001254"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUPT_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0818902.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUPT_001255"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUPT_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0818903.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUPT_001256"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUPT_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR0818904.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUPT_001257"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUPT_001257"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0818905.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUPT_001258"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUPT_001258"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR0818906.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUPT_001259"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUPT_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0818907.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUPT_001260"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUPT_001260"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0818908.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUPT_001261"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUPT_001261"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0818909.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHUPT_001262"
     CDS             11851..12015
                     /locus_tag="ACHUPT_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818910.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUPT_001263"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUPT_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0818911.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHUPT_001264"
     CDS             13524..13769
                     /locus_tag="ACHUPT_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0818912.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUPT_001265"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUPT_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0818913.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUPT_001266"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUPT_001266"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0818914.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUPT_001267"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUPT_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0818915.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUPT_001268"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUPT_001268"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0818916.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUPT_001269"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUPT_001269"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0818917.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHUPT_001270"
     CDS             19432..19785
                     /locus_tag="ACHUPT_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0818918.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUPT_001271"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUPT_001271"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0818919.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHUPT_001272"
     CDS             21085..22212
                     /locus_tag="ACHUPT_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR0818920.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUPT_001273"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUPT_001273"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0818921.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACHUPT_001274"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACHUPT_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHUPT_001275"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHUPT_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHUPT_001276"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHUPT_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0818922.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHUPT_001277"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHUPT_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0818923.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACHUPT_001278"
     CDS             26834..27583
                     /locus_tag="ACHUPT_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0818924.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACHUPT_001279"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACHUPT_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0818925.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHUPT_001280"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHUPT_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0818926.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHUPT_001281"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHUPT_001281"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0818927.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHUPT_001282"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHUPT_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0818928.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHUPT_001283"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHUPT_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0818929.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHUPT_001284"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHUPT_001284"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0818930.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACHUPT_001285"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACHUPT_001285"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0818931.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHUPT_001286"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHUPT_001286"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0818932.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMFIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHUPT_001287"
     CDS             complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHUPT_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0818933.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHUPT_001288"
     CDS             complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHUPT_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0818934.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36031)
                     /locus_tag="ACHUPT_001289"
     CDS             complement(35891..36031)
                     /locus_tag="ACHUPT_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0818935.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACHUPT_001290"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACHUPT_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0818936.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         8923 a         9695 c         9391 g         9414 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataaacatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aac
//
LOCUS       DAZOZX010000026        36770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZOZX010000026 DAZOZX010000000
VERSION     DAZOZX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.026"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            82..438
                     /locus_tag="ACHUPT_001291"
     CDS             82..438
                     /locus_tag="ACHUPT_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0818937.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            442..1569
                     /gene="dapE"
                     /locus_tag="ACHUPT_001292"
     CDS             442..1569
                     /gene="dapE"
                     /locus_tag="ACHUPT_001292"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0818938.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1597..1797
                     /locus_tag="ACHUPT_001293"
     CDS             1597..1797
                     /locus_tag="ACHUPT_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0818939.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1907..2605)
                     /gene="ypfH"
                     /locus_tag="ACHUPT_001294"
     CDS             complement(1907..2605)
                     /gene="ypfH"
                     /locus_tag="ACHUPT_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0818940.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2679..4694)
                     /gene="tmcA"
                     /locus_tag="ACHUPT_001295"
     CDS             complement(2679..4694)
                     /gene="tmcA"
                     /locus_tag="ACHUPT_001295"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0818941.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4709..5572)
                     /locus_tag="ACHUPT_001296"
     CDS             complement(4709..5572)
                     /locus_tag="ACHUPT_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0818942.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5740..6453)
                     /gene="purC"
                     /locus_tag="ACHUPT_001297"
     CDS             complement(5740..6453)
                     /gene="purC"
                     /locus_tag="ACHUPT_001297"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0818943.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6666..7700)
                     /gene="bamC"
                     /locus_tag="ACHUPT_001298"
     CDS             complement(6666..7700)
                     /gene="bamC"
                     /locus_tag="ACHUPT_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0818944.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7717..8595)
                     /gene="dapA"
                     /locus_tag="ACHUPT_001299"
     CDS             complement(7717..8595)
                     /gene="dapA"
                     /locus_tag="ACHUPT_001299"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0818945.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8741..9313
                     /locus_tag="ACHUPT_001300"
     CDS             8741..9313
                     /locus_tag="ACHUPT_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0818946.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9313..9783
                     /gene="bcp"
                     /locus_tag="ACHUPT_001301"
     CDS             9313..9783
                     /gene="bcp"
                     /locus_tag="ACHUPT_001301"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0818947.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10036..10653
                     /gene="hyfA"
                     /locus_tag="ACHUPT_001302"
     CDS             10036..10653
                     /gene="hyfA"
                     /locus_tag="ACHUPT_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0818948.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10653..12670
                     /gene="hyfB"
                     /locus_tag="ACHUPT_001303"
                     /pseudo
     CDS             10653..12670
                     /gene="hyfB"
                     /locus_tag="ACHUPT_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12681..13628
                     /gene="hyfC"
                     /locus_tag="ACHUPT_001304"
     CDS             12681..13628
                     /gene="hyfC"
                     /locus_tag="ACHUPT_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0818949.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13645..15084
                     /gene="hyfD"
                     /locus_tag="ACHUPT_001305"
     CDS             13645..15084
                     /gene="hyfD"
                     /locus_tag="ACHUPT_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR0818950.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15096..15746
                     /gene="hyfE"
                     /locus_tag="ACHUPT_001306"
     CDS             15096..15746
                     /gene="hyfE"
                     /locus_tag="ACHUPT_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0818951.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15751..17343
                     /gene="hyfF"
                     /locus_tag="ACHUPT_001307"
     CDS             15751..17343
                     /gene="hyfF"
                     /locus_tag="ACHUPT_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0818952.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            17333..19047
                     /gene="hyfG"
                     /locus_tag="ACHUPT_001308"
                     /pseudo
     CDS             17333..19047
                     /gene="hyfG"
                     /locus_tag="ACHUPT_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19057..19602
                     /gene="hyfH"
                     /locus_tag="ACHUPT_001309"
     CDS             19057..19602
                     /gene="hyfH"
                     /locus_tag="ACHUPT_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0818953.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19599..20357
                     /gene="hyfI"
                     /locus_tag="ACHUPT_001310"
     CDS             19599..20357
                     /gene="hyfI"
                     /locus_tag="ACHUPT_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0818954.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20350..20763
                     /gene="hyfJ"
                     /locus_tag="ACHUPT_001311"
     CDS             20350..20763
                     /gene="hyfJ"
                     /locus_tag="ACHUPT_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0818955.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20793..22805
                     /gene="hyfR"
                     /locus_tag="ACHUPT_001312"
                     /pseudo
     CDS             20793..22805
                     /gene="hyfR"
                     /locus_tag="ACHUPT_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22827..23699
                     /gene="focB"
                     /locus_tag="ACHUPT_001313"
                     /pseudo
     CDS             22827..23699
                     /gene="focB"
                     /locus_tag="ACHUPT_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23793..24854)
                     /locus_tag="ACHUPT_001314"
     CDS             complement(23793..24854)
                     /locus_tag="ACHUPT_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0818956.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25067..26530
                     /gene="bepA"
                     /locus_tag="ACHUPT_001315"
     CDS             25067..26530
                     /gene="bepA"
                     /locus_tag="ACHUPT_001315"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0818957.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26551..26910
                     /gene="arsC"
                     /locus_tag="ACHUPT_001316"
     CDS             26551..26910
                     /gene="arsC"
                     /locus_tag="ACHUPT_001316"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0818958.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(27043..27744)
                     /locus_tag="ACHUPT_001317"
     CDS             complement(27043..27744)
                     /locus_tag="ACHUPT_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0818959.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27839..29128)
                     /gene="uraA"
                     /locus_tag="ACHUPT_001318"
     CDS             complement(27839..29128)
                     /gene="uraA"
                     /locus_tag="ACHUPT_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0818960.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29214..29840)
                     /gene="upp"
                     /locus_tag="ACHUPT_001319"
     CDS             complement(29214..29840)
                     /gene="upp"
                     /locus_tag="ACHUPT_001319"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0818961.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30164..31201
                     /gene="purM"
                     /locus_tag="ACHUPT_001320"
     CDS             30164..31201
                     /gene="purM"
                     /locus_tag="ACHUPT_001320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0818962.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31201..31839
                     /gene="purN"
                     /locus_tag="ACHUPT_001321"
     CDS             31201..31839
                     /gene="purN"
                     /locus_tag="ACHUPT_001321"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0818963.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            32011..34077
                     /gene="ppk1"
                     /locus_tag="ACHUPT_001322"
     CDS             32011..34077
                     /gene="ppk1"
                     /locus_tag="ACHUPT_001322"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0818964.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            34082..35623
                     /gene="ppx"
                     /locus_tag="ACHUPT_001323"
     CDS             34082..35623
                     /gene="ppx"
                     /locus_tag="ACHUPT_001323"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0818965.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35662..>36770)
                     /gene="pdeF"
                     /locus_tag="ACHUPT_001324"
     CDS             complement(35662..>36770)
                     /gene="pdeF"
                     /locus_tag="ACHUPT_001324"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0818966.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8515 a         9722 c         9560 g         8973 t
ORIGIN      
        1 attttctgat aatcatttgc aggcaaaatg ttttcaccct taaatgagta tttattctca
       61 taaatcgaaa aaggattcat tatggttaca ctttacggca tcaaaaattg tgacaccatt
      121 aaaaaggctc gccgttggct ggaagccaat aacatcgact atcgttttca tgattaccgc
      181 gtcgatgggc tggacagcga attattgaac ggttttatca acgaattagg ctgggaagcg
      241 ttactcaaca cccgtggtac aacctggcgt aaactggacg aaactacccg caataaaatc
      301 accgatgcgg cctctgcggc ggcattaatg actgaaatgc ctgcaattat caaacgtcca
      361 ttgctctgcg cgcctggtaa gcctatgctg ctgggtttca gtgattccag ttatcagcaa
      421 tttttccatg aggtgtagtc tatgtcgtgc ccggttattg agctgacaca acagcttatt
      481 cgccgccctt ccctgagtcc tgatgacgca ggatgtcagg ctttgttgat tgaacgtttg
      541 caggcgatcg gttttaccgt tgaacgcatg gattttgccg atacgcagaa tttttgggca
      601 tggcgtgggc aaggtgaaac gttagccttt gccgggcata ccgacgtggt gccgcctggc
      661 gacgccgatc gttggatcaa tccgccgttt gaacccacca ttcgtgacgg catgttattc
      721 gggcgcggtg cggcagatat gaaaggctcg ctggcggcga tggtggtagc agcagaacgt
      781 tttgtcgcac aacatcccaa ccatacgggg cgactggcat ttctgatcac ttctgatgaa
      841 gaagccagtg cccacaacgg tacggtaaaa gtcgtcgagg cgttaatggc acgtaatgag
      901 cgtctcgatt actgcctggt cggcgaaccg tcgagtatcg aagtggtagg tgatgtggtg
      961 aaaaatggtc gtcgcggatc gttaacctgc aacctcacca ttcatggcgt tcaggggcat
     1021 gttgcctacc cacatctggc tgacaatccg gtacatcgcg cagcaccttt ccttaatgaa
     1081 ttagtggcta ttgagtggga tcagggcaat gaattcttcc cggcgaccag tatgcagatt
     1141 gccaatattc aggcgggaac gggcagtaac aatgttattc cgggtgaact gtttgtgcag
     1201 tttaacttcc gcttcagcac cgaactgact gatgagatga tcaaagcgca ggtacttgcc
     1261 ctgcttgaaa aacatcaact gcgctatacg gtggattggt ggctttccgg gcagccattt
     1321 ttgaccgcgc gcggtaaact ggtggatgcg gtcgttaacg cggttgagca ctataatgaa
     1381 attaaaccgc agctactgac cacaggcgga acgtccgacg ggcgctttat tgctcgcatg
     1441 ggggcgcagg tggtggaact cgggccggtc aatgccacta ttcataaaat taatgaatgt
     1501 gtgaacgctg ccgacctgca gctacttgcc cgtatgtatc aacgtatcat ggaacagctc
     1561 gtcgcgtgat gagtggttct gcaagaggaa ataagcatgg actggctggc taaatattgg
     1621 tggattctgg tgattgtctt tttggtaggc gtcctgctga acgtgattaa agatctcaag
     1681 cgcgtcgacc ataagaaatt tctcgccaac aagccggagc ttcccccgca tcgtgatttc
     1741 aacgataagt gggacgatga cgacgactgg ccgaaaaagg atcaaccgaa gaagtaagct
     1801 gagtgctcaa ccgtaggccg gataaggcgt taacgccgca tccggcaatg gtgaacgatc
     1861 cctgatgcga cgctcacgcg tcttatcagg ccaggtaatc tgtcttttac atcatttcaa
     1921 tcacatcatc atcgccaggc ttaccgccac ttagcgcttg gtcgaaataa tgtttcggaa
     1981 tggtataacg caagtgatcc agggcaaatt gcatactgcg attgtcgatc gcatggccta
     2041 aatcctccac gatatccagc gtcacgtcac caccggcact gattaacgct tcctgcgccg
     2101 caactgcatg cgccagatcg ataaccggat cttcaccacc atgaatcaag tgaattgtcg
     2161 tggctgtaga cgcggtctcc ggcaggctcg catagcgtcc gttaaaggca atgacgcgcg
     2221 aagcaaggcc cggttcggct ttaatgctct ccagcgccat aatcgcgcct tgtgagaaac
     2281 caattagcgc cgtggcgtta gcccctactc cgctctgttt ctgccagtag cgtaccgttt
     2341 caataaacgt cggcatgatg gcatccacac gcgcctggcg attatcttcc gtaataccct
     2401 gcactgaaaa ccactgacgc cccgccggat taccgctcgg ctccgcgcca ccgacactca
     2461 ccaccagcgc atcaggaaac agcggtgcaa accagctgcc tatttccccc attgccaccg
     2521 ggttatcccc gacaccgtgg aaaagcagta acaactgttg tgcaggttta tccgggcttt
     2581 gaacaacaaa atggtcatgt ttcatggcga actccttaac tgatgtcatt aattttacgc
     2641 cgctgactca caatgaccat gggagaaaat tgaaggagtc agtgaaaaaa ttgccattgc
     2701 gttatgcgat cgcgcagacg ctcagtgcga acctcattta gtgcgaacag cgcctgcgcg
     2761 gcctcttccc gctggcgaac cagtaacatc ttgcggcctg aaagtttaag tgtggtacat
     2821 aactgcgcgt cactggcgtt tttctgtaaa cgcccacgca gcgccggtaa tgccagttca
     2881 ctggtttgta gcagacgcaa taagcaacct aacgatgtta atagcggacg atgagcgaaa
     2941 gcaaaaccgg ccagttcaag ccagtcgtcg tcagaaagga tggcatcgtt tagtggatca
     3001 acaggaagca tttcgccatt ccactgcgcg agagcttgcg catcgcgacg taaacggtaa
     3061 tgctcacgtt cagccagctg tttacccgca tcactcatcg gtaacagcgc catcgccgta
     3121 tagcaaccgc tgctggcttc ccgatgatta cccatccgca ccagcacaaa accgcagcgt
     3181 tgccagaaac gccataactc cccagtgtaa ccaaaactca ccgaaagata gtcgaggtca
     3241 tgcgtatatt gcaaagctcc agcaataagt tgtcgccctg tgccttcccg ctgacgagcc
     3301 ggatgaactg ctatccggct aacccgcctt ccgcgcaatg tcgccgccag tggattgctg
     3361 ccgtgcgccg ccagcgactg ggccaccaga ttaccccgcg ggcgacgaaa acctgcccat
     3421 accgcctgac tgagttcttg agataatccg ccctcatcca ccagccacaa cgccccggca
     3481 atctcgtttt cgccagccgc ctgtaaaaaa tgttgccctg gtgcatccat catccggcgt
     3541 aaatccagcg gcgaggtccg gtagtgcgca ccagacaata actgataaac ctttaacggc
     3601 gtttctggct cgcttcgcca taacgtttgt tcaaatgcgg aaatgacgat attgccttgt
     3661 ggtgtatggg tgaagttttc atcgttaaaa accagtgcct cactaaccat tttttccagc
     3721 gggcatccct gtgcccagcg gatcggctgt tgcagttcaa aacggtgtaa atgcggaaag
     3781 cgagcgcaaa atttcagcaa aaaaccacgt ccggtacctt cgtagccctg caccgtagtg
     3841 gttaacaacg ttcgaggaaa acgcgatacc agttgatgca acaacggcgc aggtatggct
     3901 gcggcttcgt cgaccaccag ccagtcggct tgctcatcgc tggctaacaa ggcatccggt
     3961 gcaataaagc gaaacttctc gcccgcaaat tgtgccagta catccgttgc cgcttttgcg
     4021 ggcgcggtga caatcgcact gcccgcaata cgagaaatga gttgccctgc caacgccgac
     4081 ttaccgcgcc cacgcgcagc cgttaccgct gccacgcccg gcggcatggt cagtagctgc
     4141 tgtaagagtt gctgttgttc tggttgtggt gcgccggtcg cggggtgcca gtcagtacgg
     4201 ggagtaaaat gcgccaacga gaacggctgg ttttgccgcc agaggatagc gtcgttatcc
     4261 gccgtaagta cgcgtttgag atgctggaca aaatgcggcg tcgcaatagg gtcagggcaa
     4321 tcactccagc gcagcgagtc ggcatcaggt tggttttccc actcttccca tacagggagt
     4381 aacaaaacca gccagcttcc cgctttcaac gttccgctaa gtgctgcaaa ggcagcggca
     4441 tcaaagccgt ggcgggcgtc gaataccgca tgccggaact cgcgcccaag taaagtttgt
     4501 agcgccgagg gtgaacagtg gttttcagca tctagctgcg gcgaaatcca cagccagtcg
     4561 ccaggtaagg catcacgcaa cttaagagca tgatcaaaac accaaccctc ttccccgctc
     4621 aacaccagca agcggcagat cccttcacgt ttcatttgcg ctgttaatgt gtaaagcgca
     4681 gtcagttcag ccatccctgc cccggaaatt aaatgctttt accaaaagta ttgcattgtg
     4741 ccggatcgcc gctgtcgaaa ccacgtttaa accagctgta gcgttgctga gaagtgccat
     4801 gtgtgaaact gtctggtact actcgcccct gactttgctg ttgtaaacgg tcatcgccga
     4861 tggcctgcgc cgcattcagc gcctcttcca gatcgccagt ttccagaacg ccttgctgct
     4921 gcatactatg cccccagaca ccggcaaaac agtcggcctg gagttccata cgcacagata
     4981 agcggtttac ttccgcctgc gtcgcgtttt gttgcagttg acgaactttc ggctcgatgc
     5041 ctaacagttt ctgcacatga tgaccgactt catgggcgat aacgtacccc tgggcaaaat
     5101 cgccatccgc gccaagtttg tctttcatgt catcatagaa ggagagatcg atataaactg
     5161 tgccatccgc cgggcaatag aacggcccca taatggactg gcccgccccg cagccggtgc
     5221 gcgtcattcc acggtacatg accagcttcg gttgctgata ggtcttaccc atcttttcga
     5281 actgttgtcc ccaggtgtct tccgtggttg cctgaatcac cgaggtgaat tttgcggctt
     5341 cgtcttcatt agggctaatt gaccgcgttg attgttgttg agaaaccggc tgcccggtca
     5401 tcaacccggt taaatcaacg ccatagtagc ctgcaaccag cacgactatc agtaaaatca
     5461 gcccgccttt accgctcggc agacggaaac cgggaccgcc cattgacgga ccgccagagc
     5521 tgttgcgcct gtcttcaaca ttgtcacttt cacgtcgccc ttgccaacgc atagatacct
     5581 caacaatata ttcattatag aaatgatcgt aggtggttca gaggaagatt accacaggaa
     5641 agcgggatga aatggcggag gaatgagcgt tcaatgcata tattccaaga gcggttgtct
     5701 tgccgtgcca gctgcacggc aagatgatga acagaaaaat cagtccagct gtacacccag
     5761 gcggcgggcg acggcttcat aggcttcgat caggccaccg aggctctggc ggaaacggtc
     5821 tttgtccatt ttctccagtg tttctttgtc ccacaggcgg ctgccgtccg gggagaactc
     5881 atcgcccagt accacttcac ctttgtacag accaaactcc agcttgaagt cgaccagaat
     5941 cagaccagca tcatcgaaca gttttttcag cacgtcgttc gctttgtagg tcagctcttt
     6001 catacgcgcc aggttctctt tgctcaccca gccaaaggtt tcgcagtaag attcgttgac
     6061 catcggatcg tgcatggcat cgtttttcag gaacagatcg aacagcggcg ggttcagctc
     6121 aataccttct tcgattccaa gacgtttcac tagagagcca gcagcacggt tacgcacgac
     6181 acactcaacc ggcaccatat ccagcttttt caccagacat tcggtatcgg agagcagacg
     6241 ctccatttga gtcgggatac ccgcttcagc cagtttgctc ataatgaagt agttgaactt
     6301 gttgttcacc atacctttgc gatcaaactg ctcaatgcgc gcgccatccc ctgctgacgt
     6361 atcattgcgg aattcgagca ccaacaggtc cgggttttcc gtgctgtata cggttttcgc
     6421 tttaccacga tacaactcag cttgcttttg catctttatc actcctgggt gtgaattaac
     6481 gttttaaaat cttttgctgt ctggtgtgcc ggatgttttg tcggatgcgg cgtgaacgcc
     6541 ttatccgaac tacgtccgac aaacaaattt cgtgcgaatt accgctaaaa tcgcgttttc
     6601 ctgccgacgc acacgtttgc gtatcatatc agaaaaaagg gccggatgat tccagccctg
     6661 tatttttact tgctaaacgc agcctggaag acagctacca gcgcgtcgtt ctgactctga
     6721 gtcagagtat gacctttcgg atcgatgaac tgtaggctgc tgcggttatc taaatcgcca
     6781 acctgcagtt tatagtcacc ggatgccagg cctggatcgc tcgcgcccag ttcctgccag
     6841 tcgctgtcag acagcggctt ataagttacg gccatgttgc cctgcgaacg ggtgctgtcg
     6901 gtcactttca tgcccacttt ttccagcgcc gctggcagac gttgccaaac cacattgaac
     6961 ggcccgcgta cgaccagcat tggtaaaccg gtgtcatcag ctgcactttg tacgtccata
     7021 gtggtggagg cacgattttg cgcagcgttc gcggcgtcag tggcagattt atccagaccg
     7081 gcggaaataa cgttcatcat ctccgtgctg taacgctgca tggaagccgc gtctgcaacc
     7141 ggtttgcccg cctgttccag gttcagcagt ttaaccgtaa ccgcctgctg ataaccctgc
     7201 ggcttaacag agatttgata acgaccacga tactgctcgt cttcgtccag acggttccat
     7261 tgtacccaat cggtggtcag tgtctgacca gcatcatcac gttgggtgat ggtgtagttt
     7321 ttcgcctgca gcacgctaac cacctgcggc cacagagtat tgccacgacc attttccacc
     7381 agcaatgaag cggtatcgcc cgtgaactgg gtacgcgcgc cagaaaccag tgccaacggc
     7441 tgggctggtg gacgaatgtc cagcgcctta ccgacagcac cactaccgtt ggtcaccggg
     7501 attgcataat caccggaggt caccggcaaa atcattccag ccggggcatg aagctccgca
     7561 agcggtgccg cttccaggta ggcttcatca ccactgacct gacgcttata gcgtgagtca
     7621 gaactacagg cagcgagtaa taaaacaagc gaaacacccg caacctttgc caggcgcgac
     7681 ttttgaacag agtaagccat caaatctccc taaactttac agcaaaccgg catgcttaag
     7741 cgccgctctg accgtctcac gaccactgtc ggtgattggt gtcattggca ggcgcagcgt
     7801 atcggtcgcc acaagaccca gttccttaca tgcccatttc accgggattg gattgggttc
     7861 gacaaatagt ttgttgtgta atggcatcag acgctgatta ataacgcgtg cctcggcaaa
     7921 atgcccttct gctgccagtt tgcacatctg ggccatatca cgcgctgcga cgttagccgt
     7981 aacggaaata actccatgac cgcccaattg catgaagtcc agcgcgctcg catcatcgcc
     8041 gctcagcaga acaaaatcat ctgaaaccag ctctttgatc tggtttacac gcgttaagtt
     8101 ccctgttgcc tctttgattc cgataatatt ttttactttc gccagacggc ccaccgtttc
     8161 cgggagcaga tcgcagccag tacgggacgg cacattatac agaatttgcg gcaggtcagt
     8221 atgctcagcg atggctttga aatgctgata caaaccttct tgcgacggac gattgtagta
     8281 aggggttacc gtcaggcagc cgacgatacc actgtcattg aagcgctgcg tcaggctaat
     8341 ggcttccgca gtagcgttag cgccggtccc ggcaattacc ggaatgcgcc catcagccag
     8401 atccagcgtc atcatcacca catcagcatg ttcgtcatga tttaaggtag cggactcgcc
     8461 agtggtgcca acagaaacga tcgccgaagt accgctggcg acatgataat caatcagttt
     8521 tttcaagcta gcccgacaga cattaccttt ttcatccatc ggagtaacaa tcgcgacaat
     8581 acttcccgtg aacatgggcc atcctctgtg caaacaagtg tctcaatggt acgtttggta
     8641 tggcattaaa agcaagcaga cagaaccgtt ctgattgttg tatgcatgat ttttttatgc
     8701 tttccttaag aacaactcac cccttaaagg aataaccagt ttgacactgt catcgcaaca
     8761 ttatctggtg atcactgcgt tgggtgccga tcgccctgga attgtgaaca ccatcacccg
     8821 tcatgtcagt agttgcggct gtaatattga agacagtcgc ctggcgatgc tgggagaaga
     8881 gttcacgttt atcatgctgc tttccggttc atggaatgcc atcactctga ttgaatcaac
     8941 gttaccgttg aaaggtgccg aactggatct tttaatcgtg atgaagcgca cgacggcgcg
     9001 tccgcgtccg ccaatgccag catctgtctg ggttcaggtc gatgttgcag actccccgca
     9061 tttaattgaa cgcttcacag cacttttcga cgcgcatcat atgaacattg cggagctggt
     9121 gtcgcgcaca caacctgctg aaaatgaacg ggctgcgcag ttgcatattc agataaccgc
     9181 ccacagcccc gcatctgcgg acgcagcaaa tattgagcaa gcgttcaaag ccctatgtac
     9241 agaactcaat gcacaaggca gtattaacgt cgtcaattat tcccaacatg atgaacagga
     9301 tggagttaag taatgaatcc actgaaagcc ggtgatatcg caccgaaatt tagcttgccg
     9361 gatcaagacg gagaacaagt taatttgacc gacttccagg gacagcgtgt tctggtttat
     9421 ttctacccga aagccatgac ccccggctgt accgtacagg cctgcggctt acgcgataac
     9481 atggatgagt tgaaaaaagc gggcgttgat gtgctgggta tcagcaccga taaacccgaa
     9541 aaactctccc gttttgcgga aaaagagctg cttaacttta cgctcctgtc tgatgaggac
     9601 caccaggtgt gcgaacaatt cggcgtctgg ggtgaaaagt ccttcatggg caaaacctac
     9661 gatggtattc atcgcatcag cttcctgatt gacgctgatg gcaaaatcga acatgtcttt
     9721 gacgatttca aaaccagcaa tcaccacgac gttgtgctga actggctgaa agaacacgcc
     9781 tgattacttt gctccattcc gtgctggctg cgcttgcggc cagcatacct cacttctcgt
     9841 gatcaagatc acattctcgc tttcccctgc gacacgggtg tcgaatccat tttttgctga
     9901 acgttaatga ccatcatttt tgtaccgttc agaatccagt taatacataa cttattgaat
     9961 agattgagtt aatcagaatg gcatccttta tacaatatga aatgcaatgt ttcatatcat
    10021 tttcaaggag ccgacatgaa ccgctttgtg gtggccgaac cactgtggtg tacaggatgt
    10081 aatacctgtc tcgctgcctg ttcggacgtg cataaaacgc aaggtttaca gcaacacccg
    10141 cgcctggccc tggcgaagac gtcaacaatc actgcccctg tcgtgtgtca tcactgtgag
    10201 gaagcccctt gcctgcaggt ctgcccggtc aatgccatct ctcagaggga tgatgcgatc
    10261 caactcaacg aaagcctctg tattggctgc aagctttgcg ccgtggtctg cccatttggc
    10321 gcaatcagcg cttcaggaag ccgtccggtg aatgcccatg cgcaatatgt ttttcaggct
    10381 gaaggctcac tcaaagacgg cgaagaaaac gtgccgcccc aacatgcgtt gctgcgctgg
    10441 gaaccgggtg tccagaccgt cgcggtgaaa tgcgacctgt gtgatttctt gccagaaggt
    10501 ccggcctgcg ttcgcgcttg cccgaatcag gcgttacggc tgatcaccga cgatagcctg
    10561 caacgtcaga tgaaagaaaa acagcgcctt gccgcaagct ggtttgccaa tggcggggag
    10621 tctccccttt ccctcactca ggagcaacac taatggatgc cctgcaatta ttaacctggt
    10681 cgctgatcct ctatctgttt gccagtctgg cttcgctgtt tttactcggt ctggacagac
    10741 tggctattaa gctttccggc atcacatcgc tggtgggcgg cgtgattggc atcatcagcg
    10801 gaattacgca attacatgct ggtgtaactt tagtcgccca ttttgccacc ccttttgact
    10861 ttgccgattt aaccctgcga atggatagcc tctcggcatt tatggtgcta gttatctcct
    10921 tgctggtggt ggtttgttcg ctctattcat tgacttatat gcgcgaatac gagggcaaag
    10981 gcgcggcggc gatgggcttc tttatgaatc ttttcatcgc atcgatggtt gccctgctgg
    11041 tgatggacaa cgctttttgg ttcatcgtgc tgtttgaaat gatgtcgctg tcttcctggt
    11101 ttctggtcat tgccaggcag gataaaacgt cgatcaacgc tggcatgctc tactttttta
    11161 tcgcccacgc cggatcggtg ctgattatga tcgccttctt gctgatgggg cgcgaaagcg
    11221 gcagcctcga ttttgccagt ttcgcacgct ttcactttct ccggggctgg cgtcggcggt
    11281 gtttctgctg gcctttttcg gttttggcgc gaaagccggg atgatgccgt tgcacagctg
    11341 gttgccgcgc gctcaccctg ccgcaccatc gcacgcttcg gcgttgatgt ctggcgtaat
    11401 ggtcaaaatc ggtattttcg gcatcctgaa agtagcgatg gatctgctgg cgcaaacggg
    11461 tttgcctctg tggtggggca ttctggtgat ggcgatcggc gcaatctccg cgctcctggg
    11521 cgtgctatat gcgctggcgg aacaggatat caaacggctg ctggcctgga gtaccgtcga
    11581 aaacgtcggc attattttgc tggcagtcgg tgtggcgatg gacggtctgt cactgcacga
    11641 cccgctgctc accgtggttg gactgctcgg cgcactgttt catctgctca accatgcgct
    11701 gttcaaaggg ctgctatttc tcggcgcgga cgcgattatt tcgcgtttgc atacccacga
    11761 catggaaaaa atgggggcac tggcgaaacg gatgccgtgg acagccgcag catgcctgat
    11821 tggttgcctc gcgatatcag ccattcctcc gctgaatggt tttatcagcg aatggtacac
    11881 ctggcagtcg ctgttctcac taagtcgtgt ggaagccgta gcgctacaac ttgcgggtcc
    11941 tattgctatg gtaatgctgg cagtcactgg tgggctggca gtaatgtgct tcgtcaaaat
    12001 gtacggtatt actttctgtg gtgcgccgcg cagtacacac gctgaagagg cacaggaagt
    12061 gccaaatacg atgatcgtcg ccatgctact gctcgcggca ctctgcgtat taattgcgct
    12121 tagtgccagt tggctggcac cgaagataat gcatattgcc catgcgttta ccaatacccc
    12181 tcccgtcact gtcgccagcg gaatagcact tgtacccggc acgtttcata cacgggtcac
    12241 tccttcatta ctgttgctgt tactactggc gatgcctttg ctgccaggcc tttactggct
    12301 gtggtgtcgt tcgcgccgcg cagcgtttcg tcgcacagga gatgcctggg catgcggcta
    12361 cggctgggaa aacgcgatgg ctccgtcagg caatggcgtg atgcagccgc tgcgtgtggt
    12421 cttctctgcg ttatttcgtc tacgacaaca gctcgaccct acgctgaggc taaataaagg
    12481 tcttgcgcac gtcaccgcca gggctcagag cacagaaccc ttctgggatg agcgggtgat
    12541 ccgccccatc gtgagcgcca cccaacggct ggtcaaagaa atacagcatc tgcaaagcgg
    12601 cgactttcgt ctctattgcc tgtatgtggt cgccgcactg gttgtgctgc taatcgctat
    12661 tgccgtctaa ggaaatcacc atgagacaaa ctctttgcga cggatatctg gtcatttttg
    12721 cgttagcaca ggccgtgatt ctgctgatgc taaccccact ttttacgggt atttcccggc
    12781 agatacgcgc gcgtatgcac tcccgccgcg ggccggggat ctggcaggat tatcgcgata
    12841 tccacaaact gtttaaacgc caggaagttg cgccgacatc ttcaggtctg atgttccgcc
    12901 tgatgccgtg ggtattaatc agcagcatgc tggtgctggc gatggcctta ccactattta
    12961 ttaccgtttc cccttttgcg ggcggcggcg atctgatcac ccttatctat cttcttgccc
    13021 tgtttcgttt tttctttgct ctttccgggc tggataccgg aagtccgttt gcgggagtcg
    13081 gtgccagtcg cgagttgacg ctcggcattc tggtcgaacc aatgcttatt ctctcactgc
    13141 tggtattggc gctgatagca ggttccacgc atatcgagat gatcagcaat acgctggcta
    13201 tgggctggaa ctcgccgcta accaccgtac tggcgttact ggcctgtggt tttgcctgct
    13261 tcattgagat gggaaaaatt ccctttgatg ttgctgaagc agaacaggaa ttacaggaag
    13321 gcccgctgac cgaatattcc ggtgccgggc tggcgctagc gaaatggggg ctggggctga
    13381 aacaggtcgt gatggcagca ctgtttgtgg ccctgtttct gccctttggg cgcgcgcaag
    13441 aactttctct cgcctgcctg ctgacttcac ttgtcgttac gctgctcaag gttttgctga
    13501 tttttgtact ggcctcaatc gcagaaaaca cgctggcacg cgggcgtttt ttactcattc
    13561 accatgtgac ctggcttggc ttcagccttg ctgcgcttgc atgggtcttt tggttaaccg
    13621 gtctgtaagg agcactgacg gaatatggaa aatcttgctc tgacgacgtt aatgctgcct
    13681 tttatcggcg cactggtcgt ttcgttttcg ccacaacgtc gggccgccga atggggggtt
    13741 ttgttcgccg cgctgaccac gctgtgcatg ttgtcgctga tctccgcgtt ttatcaggcc
    13801 gataaagttg ccgtcacgtt gacgttggtc aacgtggggg atgtggcatt atttggcctg
    13861 gtcattgatc gcgtgagtac gctgattctg tttgtggtgg tgtttctcgg tttgctggtc
    13921 acgatctact ccacgggtta tctgacggat aaaaatcgcg aacacccgca taacggcacg
    13981 aatcgttatt acgcattttt gctggtgttt atcggcgcga tggcgggact ggtactctcc
    14041 tcgacgctgc tcggtcagtt gttgtttttt gaaattacgg gcggctgctc ctgggcgttg
    14101 atcagttatt accagagcga taaagcgcag cgttcagcac taaaagcgtt gcttatcacc
    14161 catatcggtt cgttggggtt gtatcttgcc gccgccacgc tgtttttgca gaccggaacg
    14221 tttgtgctta gcgcgatgag cgagttacat ggcgacgcac gttatctggt ttatggcggc
    14281 atcctgtttg ccgcgtgggg gaaatcggcc cagctaccga tgcaagcgtg gctaccggac
    14341 gcaatggaag cgccaacacc gatcagcgcc tatctccacg ccgcatcgat ggtgaaagtg
    14401 ggcgtttaca tttttgcccg cgccattatc gacggcggca atatcccgca tgtgattggc
    14461 ggcgttggca tggtcatgac actggtcacc attctttatg gctttctgat gtatttgcca
    14521 cagcaggata tgaagcggtt gctggcctgg tcgaccatca ctcaacttgg ctggatgttc
    14581 ttcggcttgt cgctctccat cttcggctcg cggctggcgc tggagggtag catcgcctac
    14641 atcgtcaacc acgcgttcgc taaaagcctg tttttccttg tagcaggtgc gctgagttac
    14701 agctgcggca cgcgcttgtt gccgcgtctg cgtggcgtat tgcacaccct gccgttgcca
    14761 ggcgtgggtt tctgcgtggc agcgctggca attaccggtg tgccgccgtt caacggcttc
    14821 ttcagtaaat tcccgctgtt tgccgccggt tttgcgttgt cagtggagta ctggatcctg
    14881 ctgcccgcca tgattctgct gatgattgaa tcggtcgcca gtttcgcctg gtttattcgc
    14941 tggtttggtc gcgtcgtgcc gggcaaaccg agcgaggccg tcgccgatgc cgcaccgctg
    15001 ccagggtcaa tgcgcctggt gttgattgta ctgattgtga tgtcgctgat ttccagcgta
    15061 atcgccgcga cctggttgca gtaaggagat gatgaatgac cggttctatg atcgtaaata
    15121 atctggcggg actgatgatg ctgacatcgc tgtttgtgat tagcgtcaaa agctatcgcc
    15181 tgtcatgcgg attttacgcc tgccagtcgc tggtgctggt gtctattttc gccactctct
    15241 cgtgcctgtt cgccacagag caactgctga tctggtccgc cagcgccttt atcaccaaag
    15301 tgctgctggt accgttaatc atgacttacg ctgcacgaaa tattccccag aacatcccgg
    15361 aaaaagcgtt attcggtccg gcaatgatgg cactgctcgc ggcgttaatt gtcctgcttt
    15421 gcgcatttgt cgttcagccc gtgaagctac cgatggctac cgggctgaaa ccggcgctgg
    15481 cggtagcgtt aggtcatttt ctgcttggcc tgctgtgcat tgtcagccaa cgcaatatcc
    15541 tgcggcaaat ttttggttac tgcctgatgg aaaacggctc ccatctggtg ctggcgcttc
    15601 ttgcctggcg agcaccggaa ctggtggaaa taggtatcgc taccgacgcc atcttcgccg
    15661 tcattgtgat ggtgttactg gcaagaaaaa tatggcgtac ccacggcacg ctggacgtga
    15721 acaacttgac cgcgctgaag ggataatgag atgagttatt ctgtgatgtt cgctttactc
    15781 ctgctcacgc cgctgctttt ttcgctgctc tgttttgcct gccggaaacg ggggctctct
    15841 gcgacttgta cggtgactgt attacatagc ttagggatca cactgctgct gattctagca
    15901 ctctgggtgg tccaaactgc cgctgatgca ggagaaatat tcgctgcggg actgtggctt
    15961 catattgatg gtctgggcgg tttgttcctc gccattcttg gtgtgattgg ctttctcacc
    16021 ggtgtttact cgattggcta catgcgtcat gaagtggcac acggcgagct ttcacccgtt
    16081 acactgtgcg attactacgg tttcttccat ctgtttttgt tcaccatgct gctggttgtt
    16141 accagcaata acctgattgt gatgtgggcg gcgatcgaag ccaccacctt aagctcggcg
    16201 tttctggtag gcatttacgg tcagcgttca tcgctggaat cagcatggaa gtacatcatt
    16261 atttgtactg ttggtgtcgc ttttggtctg ttcggtaccg tgctggtata cgccaacgcc
    16321 gccagcgtta tgccgcaggc agaaatggcg atattctgga gcgaggttct taagcaatcg
    16381 tccttgcttg acccaacatt aatgctgttg gcctttgtgt ttgtgctaat tggctttggt
    16441 accaaaaccg ggctatttcc catgcacgcc tggctgccgg atgctcacag tgaagcgccg
    16501 agtccggtca gcgccctgct ctccgccgta ttgctgaact gcgcgctgtt ggtgctgatt
    16561 cgctattaca tcattatttg ccaggccatc ggcagcgatt tccccaaccg gttgttgctc
    16621 atcttcggca tgttgtcggt tgccgtggcg gcatttttca ttctggtaca gcgggacatt
    16681 aagcgtctgc tggcgtactc cagcgtggag aacatggggc tggtcgcggt ggcgctaggc
    16741 attggcgggc cgctgggaat ttttgccgcg ctgctgcaca ccttaaacca cagtctggca
    16801 aaaacgctgc tgttctgcgg ttccggcaat gtactgctca agtacggcac gcgcgatctc
    16861 aacgtcgtct gtgggatgct caaaatcatg ccatttaccg ccgtgctgtt tggcggcggt
    16921 gcgctggcgc tggcgctggc gctggcaggg atgccgccct tcaacatttt tcttagcgaa
    16981 tttatgaccg ttaccgcagg actggcacgt aatcacctgc tgattatcgt cctgctgtta
    17041 ttgctgttaa cgctggtgct ggcgggcctg gtacggatgg ctgcgcgggt gttaatggcg
    17101 aaaccgccgc aggccgttaa ccggggtgat ctcggctggt tgaccacctc gccaatggtg
    17161 attctgctgg tcatgatgct ggcgatggga acgcatattc cacaacctgt catcaggatc
    17221 ctggcgggcg cttccactat agtcctctca gggacgcacg acctgcctgc acaacgtagc
    17281 acctggcatg attttttgcc ttcaggcacc gcatctgttt cggagaaaca cagtgaacgt
    17341 taattcatcg tcaaatcgtg gcgaagcgat tctcgccgcc ctgaaaacgc agttccccgg
    17401 cgcggtgctg gatgaagagc gacaaacgcc tgaacaggtc accattacgg tgaaaatcaa
    17461 tctgctgcct gacgttgtac attatcttta ttatcaacat gatggctggc ttccagtcct
    17521 gtttggcaac gacgagcgga cacttaacgg tcattacgcg gtttattatg ccctttcaat
    17581 ggaaggggcc gaaaaatgct ggatcgtggt gaaggcactg gtcgatgccg acagtcggga
    17641 gtttccgtca gtcacaccgc gcgtccctgc cgcggtctgg ggcgagcgag aaattcgcga
    17701 tatgtacggg ctgattccgg ttggcctgcc ggatcagcgt cgcctggtgt tgcccgatga
    17761 ctggccggaa gatatgcatc cgctgcgcaa agatgcgatg gattatcgac tgcgccctga
    17821 accgacgaca gattccgaaa cgtatgcgtt tatcaatgag ggcaacagcg atgcgcgggt
    17881 gatccctgtc ggcccgctgc atatcacctc ctatgaaccg ggtcacttcc gcttgtttgt
    17941 ggatggcgag caaattgtcg atgctgatta ccgcctgttt tatgtccatc gcggcatgga
    18001 gaaactggca gaaacgcgga tgggctacaa cgaagtgacc ttcttatcgg accgcgtgtg
    18061 tgggatttgc ggttttgccc acagtgtggc ctataccaac tcggttgaaa atgcactggg
    18121 gattgaggtg ccgcaacgag cacatactat tcgctcgatt ctgctggaag tcgagcggct
    18181 acacagtcat ttgctcaacc ttggcctctc ctgccatttt gttggttttg ataccggctt
    18241 tatgcaattt ttccgcgtgc gggaaaagtc gatgacgatg gcggaattgc tgaccgggtc
    18301 gcgtaaaacc tacggtctga atctgattgg tggtgttcgc cgcgatattc tcaaagagca
    18361 acgtctgcaa acgctgaaac tggtgcgcga gatgcgcgcc gacgtgtcgg agctggtaga
    18421 gatgctgctt gctacgccga atatggaaca acgcactcag ggcattggca ttctcgaccg
    18481 acaaatcgcc cgtgattata gccctgtagg gccgctgatc cgcgcagtgg ttttgcccgt
    18541 gatttgcgct ttgatcaccc ctacgccgac tacggcaata ttccaaaaac actgtttacc
    18601 tttaccggcg gcgatgtttt ctcccgcgtg atggtccgtg tcaaagagac gtttgattcg
    18661 ctggcaatgc tggaatttgc cctcgacaac atgccggata ccccactgct gaccgaaggc
    18721 tttagctata aacctcacgc attcgcgctg ggctttgttg aagcgccacg cggtgaagac
    18781 gtgcactgga gcatgctcgg tgataaccaa aaattgttcc gctggcgctg tcgtgccgcc
    18841 acctacgcca actggccggt gttgcgttac atgctgcgcg gcaataccgt ttctgacgca
    18901 ccgctgatta tcggtagcct tgatccctgc tactcctgta ccgaccgtgt gacgctggta
    18961 gatgtgcgca agcgccagtc aaaaaccgtg ctgtataaag agatcgaacg ctacggcatt
    19021 gatcgtaacc gttcgccgct gaagtaagga cagaagatgc tgaagttact gaaaactatt
    19081 atgcgcgccg gaaccgcgac ggtgaaatat cccttcgcgc cactggaggt cagcccaggc
    19141 tttcgcggga aaccggacct gatgcccagc caatgtattg cctgcggtgc ctgcgcctgt
    19201 gcttgtccgg caaatgcgct gactatccag accgacgacc agcaaaattc gcgcacctgg
    19261 cagctctatc tggggcgttg tatttactgc ggacgttgtg aagaagtgtg cccgaccaga
    19321 gccatccagc ttaccaataa ctttgaactg accgtcaccc ataaagccga tctctatacc
    19381 cacgcgacgt tccatctaca acgttgcagc cgttgcgaac gcccgtttgc cccgcaaaaa
    19441 accgtcgcac tggctgctga attgttggca cagcaacaaa atgctccaca aaaccgcgaa
    19501 atgttatggg cgcaagcgag cgtctgcccg gagtgcaaac aacgcgcaac gctgatcaac
    19561 gacgatacag atgtaccgct ggtggctaag gagcagctat gagtccagtg cttacgcaat
    19621 atgtcagcca gcccatcacg ctggacgagc aaacgcaaaa gatgaagcgg catttgctac
    19681 aggatatccg tcgctcggct tacgtttatc gcatcgattg cggcggctgc aacgcctgtg
    19741 aaatcgaaat ttttgctgcc atcacccccg tattcgacgc agaacgtttt ggcattaagg
    19801 ttgtttcatc accgcgtcac gccgatattt tgttatttac tggcgcagtc acccgggcga
    19861 tgcgtatgcc tgcacttcgg gcgtatgagt ctgcccccga tcataaaatc tgtgtttcct
    19921 acggcgcgtg cggtgtcggc ggcggtattt tccacgatct ctacagcgtc tggggcggca
    19981 gcgacaccat tgtccccatt gatgtttgga tccccggctg cccgccaaca ccggccgcca
    20041 ccattcacgg tttcgccgtg gcgctcggtt tgctgcaaca gaagattcac gcggtggatt
    20101 atcgcgatcc caccggggtg actatgcaac cgttgtggcc gcagatcccg ccatcacagc
    20161 gtatcgccat tgagcgagaa gcgcggcggc tggcgggcta tcgtcagggg cgagaaattt
    20221 gcgatcggct cctgcgccat ttaagcgacg atcctacagg aaatcgggtt aacacctggt
    20281 tgcgcgatgc cgacgatcca cgtctcaata gtatcgttca gcaactcttt cgcgtactcc
    20341 gggggttaca tgactgaaga gtgcggggaa attgttttct ggacgctgcg aaaaaagttt
    20401 gtcgccagta gcgacgagat gccggaacac agctctcagg taatgtatta ctcgctggct
    20461 atcggtcatc acgttggcgt gattgattgt ctgaatgtcg ccttccgctg cccactgacg
    20521 gaatacgaag attggcttgc actggtcgaa gaggagcaag cccgacgtaa gatgctgggg
    20581 gtgatgactt ttggtgagat tgttattgac gccagccaca ccgccctgtt gacccgggca
    20641 ttcgcgccac tggcagatga cgcgacgtct gtgtggcaag cgcgtagcat tcaattcatt
    20701 catctgttgg atgaaattgt gctggaaccg gctatctatc tgatggccag aaaaattgcg
    20761 tgagaaggat ttctcattaa taaggactgt tgatggctat gtcagacgaa gcgatgtttg
    20821 ccccgccgca aggaataaca attgaagcgg taaacggaat gctcgcggag cggttagcgc
    20881 agaaacacgg caaggcgtct ttattacgcg ccttcatccc gctgccgccg ccgttcagcc
    20941 cggtacaact tattgaactg catgttctca aaagcaactt ctattaccgc taccatgatg
    21001 atggcagcga tgtgacggca acaacagagt atcagggcga gatggtcgat tattcgcgtt
    21061 acgccgtcct tctcggcagt agtggaatgg cggagctacg ctttattcgt acccacggca
    21121 gccgttttac tccccaggat tgcacactgt ttaactggct ggcacgcatt atcaccccgg
    21181 ttctgcaatc atggctcaat gatgaagcac agcaggtggc gctgcgtttg ctggagaaag
    21241 atcgcgatca tcatcgggta ctggtggata tcactaatgc agtgctgtca catcttgatc
    21301 tcgacgatct gatcgctgac gtcgctcgtg agatccatca ttttttcggt ctggcttcag
    21361 tcagtatggt actgggcgat catcgaaaga acgagaagtt cagcctgtgg tgcagcgatc
    21421 tttctgcctc acattgtgcg tgtctgccac acaatatgcc tggcgacagt gtattgctga
    21481 cacaaacgct acaaacccga caaccgacct tgacgcaccg tgcagatgat ctgtttctct
    21541 ggcaacgcga cccgttatta ctcttacttg catctaacgg ctgcgaatct gcgctcctta
    21601 taccgcttac ctttggcaac catacaccgg gtgcattgtt gctggcgcat acctcttcca
    21661 ctctctttag tgaggaaaac tgccagctac tacaacacat agccgatcgc atcgctattg
    21721 ccgttggcaa tgccgatgcc tgccgtagaa tgaccgattt gcaggaaagt ttgcagcaag
    21781 aaaaccacca gcttagcgag cagctccttt cgaatctggg cgtcggtgac attatctatc
    21841 aaagccaggc aatggaagac ctgctccagc aggtagatat tgtggcgaag agcgacagta
    21901 cggtgttgat ttgcggtgaa accggaaccg gcaaagaggt gatcgccaga gcgatccatc
    21961 aacttagccc gcgacacgac aagccgctgg tcaaaatcaa ctgcgctgcc atccccgcca
    22021 gtcttctgga aagtgagtta ttcggtcatg acaaaggggc gtttactggt gcgattaata
    22081 cccatcgtgg tcgttttgaa attgccgatg gcggcacgtt gtttctcgat gaaattggcg
    22141 atctgccgtt agaacttcag cctaaactgc tgcgcgtatt gcaggaacgg gagattgagc
    22201 gtctcggcgg gagtagaacg atcccggtaa atgtcagagt cattgccgcc accaaccgtg
    22261 atttgtggca aatggttgaa gatcgccagt ttcgcagcga tctcttttat cgcctgaatg
    22321 tcttcccact ggaattgcct ccgctgcgcg accgtccgga agatatccct cttttagcaa
    22381 agcatttcac gcaaaaaatg gcgcgccata tgaatcgttc aattgacgcc atcccgaccg
    22441 aggcgctacg ccagttgatg tcgtgggact ggccgggcaa cgtgcgcgag ctggaaaacg
    22501 tgattgagcg agcggtactg ctgactcgcg gtaacagtct gaatttacat cttaatgtcc
    22561 gacaaagccg tttactgccg acgctaaatg aagattcagc gcttcgcagt tcaatggcgt
    22621 agttgctgca cccgacgacg ccagagaatg acgaagaaga acgtcagcgc attgttcagg
    22681 tattgcgaga aaccaatggc attgttgccg ggccccgtgg cgcggcgaca cgattaggga
    22741 tgaagcgcac cacgctgctg tcacgaatgc agcgtctggg aatctcggtt cgcgaggtgt
    22801 tgtaatctgc ttttgcagga gtatgcatga gaaacaaact ctctttcgac ttgcagttga
    22861 gcgccagaaa agcggcaatc gctgaacgga ttgccgccca taaaattgcc cgcagtaaag
    22921 tgtcggtctt tttaatggcg atgtccgctg gcgtgtttat ggcgatgtcc gctggcgtgt
    22981 ttatggcgat cggatttact ttttaccttt ccgttatcgc cgatgccccg tcttcatagg
    23041 cattaaccca tctggtgggc ggcctttgct ttacacttgg ctttattttg ctggcggttt
    23101 gcggcaccag cctgttcacc tcgtcggtaa tgacggtaat ggcaaaaagt cggggcgtta
    23161 ttagttggcg aacttggctg attaacgcac ttctggtggc ctgcggtaat ctggcaggta
    23221 ttgcctgttt cagtttgtta atctggtttt ccgggctggt gatgagtgaa aacgcgatgt
    23281 ggggagtcgc gattttacac tgcgccgagg gcaaaatgca tcatacattt actgaatctg
    23341 tcagcctcgg cattatgtgc aatctgatgg tttgcctggc gctgtggatg agttattgcg
    23401 gacgttcgtt atgcgacaaa atcgtcgcca tgattttgtc catcaccctg tttgtcgcca
    23461 gtggctttga gcactgtatc gccaatttgt ttgtgattcc gttcgccatt gccattcgcc
    23521 atttcgcccc tacctccttc tggcaactgg cgcacagtag cgcagacaat tttccggcac
    23581 tgacggtcag ccattttatt accgccaatc tgctcccggt gatgctgggt aatattatcg
    23641 gcggtgcagt gctggtgagt atttgttatc gggctattta tttacgtcag gaatcctgaa
    23701 aaatcagccc ggcgaaacag ttcgtcgggc tatttattta cgtcaggaat cctgaaaaat
    23761 cagcccggcg aaacagttcg tcgggctgaa gattattctt gcgcgatttg cccatcgggc
    23821 caggcgtgga tcacggcttt gatcagcgtc gccaatggaa tggcgaagaa tacgccccag
    23881 aatccccaca aaccaccgaa gatcaccacc gataaaataa tcaccagcgg atgcaggtta
    23941 accgcttcgg agaacaacac cggtactagc aggttgccgt ccagcgcctg aataatcaga
    24001 tacaccgcga agcagctcca gaattcagta cctgcgccaa actggaatag cgccacgcca
    24061 accaccggaa tggtcaccac aaatgcgcca atgtacggaa taagaaccga gaagccgacc
    24121 agcactgcca gcagcagcga atagttcagc ccgaagagca agaaccccag ccaggtggcg
    24181 atccccacca cgatcatctc cagcactttg ccgcggatat agttggtgat ttgttgattc
    24241 atctccttcc acacctgtcc tgccagccca cggttacgcg gcagcacccg gcgaacggcg
    24301 ttcagcatct gctctttgtc tttcagcagg aagaagacca tcaatggcac cagcaccaga
    24361 tagacggcta tggtcagcaa accgaccagc gaggcgaggg aaattttcac caccgaatcg
    24421 cccatggtca gcatccgact gcgcatattt tcggccattg catcaataat gcccgcgtcc
    24481 attaacaccg gatagcggcg cggcaacgtg gcggcaaagt cagaaagctt attgagcatc
    24541 cccggcatat cgcggattaa gtagatgccc tgttgccagg cgataggcag taccacgaac
    24601 gccatcagta gcaatatacc gacaaaaacc accaatacaa tcgacgtcgc ccagcggcgg
    24661 gagcagccaa tagattgcag gcgcacggtt ggccattcca gcaaataagc cagcacaata
    24721 gccaccagca acggagcaag caggccacta aagaaaaaga taatgccaaa tccggcaact
    24781 aaaataacca gcaaggcaat cgcttccggg tcgctaaaac ggcggcgata ccattgcatc
    24841 aacatttcga gcataaagac cttccctgaa cctcaagagc gggattgcga tccgcaattg
    24901 tatcgaaatg tcacaaaaaa gacttcgctt tttatgacgg attcaggaaa ctgaaaagtc
    24961 atttgagtgg gctaatcttc gccgttacac tcaaaggcgg cgaggtggga acgatatttc
    25021 acagtatcgg tcaaatgact atcttcagaa atacaggata gaggttatgt tcaggcagtt
    25081 gaaaaaaaac ctggttgcaa ccctcattgc tgctatgacc attggtcagg tagccccggc
    25141 gtttgccgac agcgcagaca ccttgccgga tatgggaacc tccgcaggaa gcacgctttc
    25201 cattggtcag gaaatgcaga tgggcgacta ttatgtccgc cagctacgcg gcagcgcgcc
    25261 gttaattaat gacccgctgt taacgcaata tattaattcg ctggggatgc gtctggtttc
    25321 gcatgccaat tcggttaaga caccgtttca tttttttctg atcaacaacg acgaaattaa
    25381 cgcctttgct ttctttggcg gcaacgtggt gctgcactct gccctgttcc gttattccga
    25441 taacgaaagt caactggctt cagttatggc gcacgaaatc tcccacgtca cccaacgtca
    25501 cctggcgcga gcgatggaag atcaacaacg cagcgcgccg ctgacctggg tcggcgcgtt
    25561 aggttctatt ttactggcga tggccagtcc gcaggcgggg atggcggcgc tgaccggtac
    25621 actggcggga acgcgccagg ggatgatcag tttcacccag caaaatgaac aggaagcgga
    25681 ccgcattggt attcaggtgc tgcaacgctc gggattcgat ccacaggcga tgccgacctt
    25741 cctggaaaaa ttgctcgatc aggcgcgtta ctcctcgcgc ccgccggaaa ttttattgac
    25801 tcacccgttg ccggaaagtc gtctggcaga tgcccgcaac cgtgctaatc agatgcgccc
    25861 gatggtggtg cagtcgtcgg aagatttcta tctggcgaaa gcgcgcacac tggggatgta
    25921 taattccgga cgtaaccagc tcaccagtga tttgctggat gaatgggcga aaggaaacgt
    25981 tcgtcagcaa cgagcggcgc aatatggtcg tgctttacag gcgatggaag ccaataaata
    26041 cgacgaggcg cgtaaaacgc tgcaaccgtt actggcggca gaacctggca acgcatggta
    26101 tctcgatctg gctaccgata tcgatcttgg gcaaaacaaa gccaatgagg caatcaatcg
    26161 cctgaaaaat gcccgcgatt tacgcaccaa tccggtgttg cagctcaacc tggcgaacgc
    26221 ttatctgcaa ggcggtcaac cacaagaagc ggccaatatt ctgaatcgct acacctttaa
    26281 taataaagat gacagcaacg gctgggattt gctggcacag gcggaagccg cgctaaataa
    26341 ccgcgatcag gagctggctg cgcgagcaga aggttatgcg ctcgccggac gactcgatca
    26401 ggccatttcg ctgttgagta gcgccagttc gcaggtgaaa ttaggcagcc tgcaacaagc
    26461 gcgttacgat gcgcgcatcg accagttgcg ccagctgcag aaacgcttta agccttatac
    26521 caagatgtaa ttcgcattaa aggaagaaca atgaccaaac aggtaaaaat ctaccataac
    26581 ccacgctgtt caaagagccg ggaaacgctg aacctgctga aagaaaacgg cgtggagcca
    26641 gaagtggtgc tctaccttga gacacccgcc gatgcggcaa cgctgcgcga tttgctgaaa
    26701 atgctgggga tgaatagcgc ccgtgaattg atgcgccaga aagaggatct ttataaagaa
    26761 ctaaacctgg ctgacagttc gcttagcgaa gaggcgctga ttcaggcaat ggtggataat
    26821 ccgaagctga tggagcgccc gattatggtc gcgaatggca aagcgcggat tggtcgtcca
    26881 ccggagcagg tactggaaat cgtgggttaa gatgtgctcc atcgctagtt gaagcacatt
    26941 gccggatgcg acgcttgcca cgtcttatcc ggcctacgaa tactatggat taacttcgcg
    27001 taggtcaggc gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc
    27061 tttcacaaac ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc
    27121 caacgtcata aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac
    27181 atcttccggc agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc
    27241 ttcatcagaa agtggctgca atttgtagat ctgcccccag tcgagacgcg acgcgagatc
    27301 cggtaatccc agattcaact gccgcggtgg acgatcgccg gtgatcaaca accgtgtttt
    27361 gcccgattcc agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc
    27421 tgcaatacac tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac
    27481 ttccggaaca aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg
    27541 cgacaattcc gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca
    27601 gagatagatg taaccgctat gttcctgacg cagcacgttt tgcagcgcgg ccagtaaaga
    27661 ggagttatcc cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa
    27721 agagagctgt gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa
    27781 gtttaccaca gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt
    27841 atttgtctgt tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac
    27901 tgatcagttt gaaaatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt
    27961 ttaactccgc cgcgccgatg tttaccttcg cgccactgac gccgatgatc aaaatcacgg
    28021 aagtcaggat caggttctgt gctttgttgt aatccacttt cgattcgatc aaaacacgaa
    28081 taccggaagc accaatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg
    28141 ggatcatctg gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg
    28201 ccgccccgcc gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt
    28261 ctccgtaagt ggtatttggc gtagagccaa agaagccgga gatcacggtc gacaggccgt
    28321 tagcaaacat cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag
    28381 ccgttactac caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag
    28441 tcagaatggc aaaccactcg aagcgcggcg tatagagggt tggcagtgca aaccagtgag
    28501 cattaataat cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca
    28561 gcacgccaat taaaatcggg ataattgcca ggaaaccacg aaacagcacg gaacctaaaa
    28621 ccgtgaccgc cagggtggta atagagatga tgatggtttt ggagtttggc gtttgccctt
    28681 cagccgggag taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg
    28741 caacgattgc gcccattgcc gcaggtggaa acagcacgtc caaccagccg gtccccgctt
    28801 tcttcacgat aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg
    28861 cgacttcata ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac
    28921 caagataagc cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt
    28981 taaataacag tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac
    29041 caaacatggc gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt
    29101 cactcacccc gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg
    29161 ctttactcaa aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc
    29221 gtaccaaaga ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc
    29281 agtccctggt caatcgatgc agtatacagt tcgacgtccg ggtgcgcttt ttccagcgca
    29341 gcgatacctt ctggcgcagc gaccagcacc agaactttga tgctgctgca gcccgctttt
    29401 ttcagcaggt cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc
    29461 agcgccatac gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc
    29521 gtttcttcat tacggtacat accgacaacg ctgatgcgcg cgctcggaac gttttccagc
    29581 acaccgtcca tcataccaag acccgcacgc agaattggca caacggtaat tttcttacct
    29641 ttgatctggt cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg
    29701 aggtcggcgg tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag
    29761 cgcttggtgc tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt
    29821 ttgacttcca cgatcttcat actctttctc ctttgagggg cagccacaaa aaaatcgacg
    29881 gattatacct cctttcttca gggcggcaat attattttcg ttgactttag tcaaaatgat
    29941 aacagtttga gataaagtta ttttatattc aaatggttat gaaagaagat tattccatcc
    30001 gaaaactaac ctttgccctg gcacaagtct tctttcgccg cgcgcccggg gaaaagacgt
    30061 gcaaaaaggt tgtgtaaaac agtctcgcaa acgtttgctt tccctgttag aattgcgccg
    30121 aattttattt ttctaccgca agtaacgcgt ggggacccaa gcagtgaccg ataaaacctc
    30181 tcttagctac aaagatgccg gtgttgatat tgacgcgggt aatgctctgg ttggaagaat
    30241 caaaggcgta gtgaagaaaa cgcgtcgtcc ggaagtgatg ggcggtctgg gcggcttcgg
    30301 tgcgctgtgt gcattgccgc aaaaatatcg tgaacccgtg ctggtttccg gcactgacgg
    30361 cgtaggtacc aagctgcgtc tggcaatgga cttaaaacgt cacgacacca ttggtattga
    30421 tctggtcgcc atgtgcgtta atgacctggt ggtgcaaggt gcggaaccgc tgtttttcct
    30481 cgactattac gcaaccggaa aactggatgt tgataccgct tcagcggtga tcagcggcat
    30541 cgcggaaggt tgtctgcaat cgggctgttc tctggtgggt ggcgaaacgg cagaaatgcc
    30601 ggggatgtat cacggtgaag attacgatgt cgcgggtttc tgcgtgggcg tggtagaaaa
    30661 atcagaaatc atcgacggct ctaaagtcag cgacggcgat gtgctgattg cactcggttc
    30721 cagcggtccg cactcgaacg gctattcgct ggtgcgcaaa attcttgaag tcagcggttg
    30781 tgatccgcaa accaccgaac ttgatggtaa gccattagcc gatcatctgc tggcaccgac
    30841 ccgcatttac gtgaagtcag tgctggagtt gattgaaaag gtcgatgtgc atgccattgc
    30901 gcacctgacc ggcggcggct tctgggaaaa cattccgcgc gtattgccag ataataccca
    30961 ggcagtgatt gatgaatctt cctggcagtg gccggaagtg ttcaactggc tgcaaacggc
    31021 aggtaacgtt gagcgccatg aaatgtatcg caccttcaac tgcggcgtcg ggatgattat
    31081 cgccctgcct gctccggaag tggacaaagc cctcgccctg ctaaatgcca acggtgaaaa
    31141 cgcgtggaaa atcggtatca tcaaagcctc tgattccgaa caacgcgtgg ttatcgaata
    31201 atgaatattg tggtgcttat ttccggcaac ggaagtaatt tacaggcaat tattgacgcc
    31261 tgtaaaacca acaaaattaa aggcaccgta cgggcagttt tcagcaataa ggccgacgcg
    31321 ttcggccttg aacgcgcccg ccaggcgggt attgcaacgc atacgctcat cgccagcgcg
    31381 tttgacagtc gtgaagccta tgaccgggag ttgattcatg aaatcgacat gtacgcaccc
    31441 gatgtggtcg tgctggctgg ttttatgcgc attctcagcc cggcgtttgt ctcccactat
    31501 gccgggcgtt tgctgaacat tcacccttct ctgctgccga aatatcccgg attacacacc
    31561 catcgtcagg cgctggaaaa tggcgatgaa gagcacggta catcggtgca tttcgtcacc
    31621 gatgaactgg acggtggccc ggttatttta caggcgaaag tcccggtatt tgctggtgat
    31681 acggaagatg acatcaccgc ccgcgtgcaa acccaggaac acgctattta tccactggtg
    31741 attagctggt ttgccgatgg tcgtctgaaa atgcacgaaa acgccgcgtg gctggatggt
    31801 caacgtctgc cgccgcaggg ctacgctgcc gacgagtaat gcccccgtag ttaaagcgcc
    31861 agctctgccg ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc
    31921 ccccttttct ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg
    31981 tcccgtgaat aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa
    32041 gagctcagtt ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac
    32101 ccgctgattg aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat
    32161 aaagtccgct tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac
    32221 gctcattccc gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa
    32281 ttcgacggcc tctacaacga gctattgctg gagatggcgc gcaaccagat cttcctgatt
    32341 aatgaacgcc agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat
    32401 ctgcgtcagc acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg
    32461 aaagatgatt acacctatct ggcggtggaa attatccgta gcgatagcat ccgttacgcg
    32521 ctgctggaga tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcgccg
    32581 cgtcgacgca agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt
    32641 ttcaaaggct tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat
    32701 gccgaatacg atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt
    32761 ctcaagcagc gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat
    32821 gcgctggttg aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc
    32881 ggcggtcgtt atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat
    32941 ctggtgaaca aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc
    33001 aatggttttg atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt
    33061 gagcatgtgc tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa
    33121 attaacattt accgcgtggc gaaagattca cgcatcatcg actcaatgat ccacgccgca
    33181 cataacggta agaaagtcac tgtggtggtt gagttacagg cgcgtttcga cgaagaagcc
    33241 aacattcact gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg
    33301 gggctgaaaa ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc
    33361 cgttacgcac acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac
    33421 tattcgttgc tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt
    33481 attgaaaacc cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct
    33541 cgccgtctgt tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc
    33601 agtggtatca ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat
    33661 gcggcctcca gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc
    33721 cccaatctgg aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt
    33781 gaacatgacc gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc
    33841 gccgactgga tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat
    33901 ccgcgcctga agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa
    33961 gcacgttata tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa
    34021 gtacgggcgc agttggcgat ttacgactac atcaaatcac tcgaacaacc tgaataaccc
    34081 tatgccaata cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc
    34141 aaacagtttt cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg
    34201 cctgaaacag cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga
    34261 ggcaatgacg cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc
    34321 tcctgccagc gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga
    34381 ctttctgaaa cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga
    34441 agaagcccgt ctgattttta tgggcgtgga acatacccaa ccggaaaaag gtcgcaaact
    34501 ggttattgat attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaacctat
    34561 tctcgttgaa agccgccgga tgggttgtgt cagctttgcc cagctttatt ttcctggcgg
    34621 ggtcatcaat aaagagaatt ttcagcgcgc tcgcatggcg gcagcacaaa aactggaaac
    34681 tttaacctgg caattccgta ttcagggctg gaacgttgca atgggcgctt ccggtaccat
    34741 aaaagccgcc catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga
    34801 acgtctggaa aaactggtaa aagaaatttt acgtcaccgt aatttcgcat cgctgagttt
    34861 accgggtctt tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgtgg
    34921 tgtgtttgat gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg
    34981 cgtactgtat gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag
    35041 cagcctcgcc aaccagtatc acatagacag cgaacaggcc cgacgagtgc tggataccac
    35101 tatgcaaatg tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga
    35161 ggcgctactg cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg
    35221 tttgcatcgc cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga
    35281 acagcagctg atgatggcga cactggtgcg ctatcaccgt aaagcgatta agctcgacga
    35341 tctgccgcgc tttaccttgt ttaagaagaa acagttcctg ccactgatac agctattgcg
    35401 ccttggcgta ttactaaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact
    35461 gattactgat gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc
    35521 gctggtactg cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg
    35581 gttgaaaatt gaagaagaaa gtaccccaga aatcgccgct taatgctttg cgggccgacg
    35641 agaaatgtcg gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcagcgt
    35701 atcaattaac ggttgcggct taccaataag ataaccctgc atataatcga tccccaaaga
    35761 gagcaccgcc tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt
    35821 cattcgcgcc agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat
    35881 attgcggata aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc
    35941 atagctggcg tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag
    36001 atgctgcaag gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc
    36061 aaaaataagt tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg
    36121 aaaacgagcc tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat
    36181 cttcgctctg ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact
    36241 cgacgataaa ccaaattcgt gcgcgaccgg caagaagcta ccggggccga tcagttcatc
    36301 attctcacct ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat
    36361 cggctgggcc atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat
    36421 cgcgacttta tctttcaatt cgcgttgcaa atacattgcc ccgcaacgct gcatattttc
    36481 cggggcgttg gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta
    36541 gatatgattc actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg
    36601 catcccatcc caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc
    36661 ctggtgcgat tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc
    36721 ttcacccggt tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt
//
LOCUS       DAZOZX010000027        36587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZOZX010000027 DAZOZX010000000
VERSION     DAZOZX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.027"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUPT_001325"
     CDS             <1..190
                     /locus_tag="ACHUPT_001325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0818967.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHUPT_001326"
     CDS             complement(156..1391)
                     /locus_tag="ACHUPT_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0818968.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHUPT_001327"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHUPT_001327"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHUPT_001328"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHUPT_001328"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHUPT_001329"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHUPT_001329"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHUPT_001330"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHUPT_001330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHUPT_001331"
     CDS             complement(2077..3462)
                     /locus_tag="ACHUPT_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0818969.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHUPT_001332"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHUPT_001332"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0818970.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHUPT_001333"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHUPT_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0818971.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHUPT_001334"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHUPT_001334"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0818972.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHUPT_001335"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHUPT_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0818973.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHUPT_001336"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHUPT_001336"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0818974.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHUPT_001337"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHUPT_001337"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0818975.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHUPT_001338"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHUPT_001338"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0818976.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHUPT_001339"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHUPT_001339"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0818977.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHUPT_001340"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHUPT_001340"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0818978.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHUPT_001341"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHUPT_001341"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0818979.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHUPT_001342"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHUPT_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0818980.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHUPT_001343"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHUPT_001343"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0818981.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHUPT_001344"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHUPT_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0818982.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHUPT_001345"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHUPT_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0818983.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHUPT_001346"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHUPT_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0818984.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHUPT_001347"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHUPT_001347"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0818985.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHUPT_001348"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHUPT_001348"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0818986.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHUPT_001349"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHUPT_001349"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0818987.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHUPT_001350"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHUPT_001350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0818988.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHUPT_001351"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHUPT_001351"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0818989.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHUPT_001352"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHUPT_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0818990.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHUPT_001353"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHUPT_001353"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0818991.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHUPT_001354"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHUPT_001354"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0818992.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHUPT_001355"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHUPT_001355"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0818993.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHUPT_001356"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHUPT_001356"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0818994.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHUPT_001357"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHUPT_001357"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0818995.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHUPT_001358"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHUPT_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0818996.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHUPT_001359"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHUPT_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0818997.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACHUPT_001360"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACHUPT_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHUPT_001361"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHUPT_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0818998.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHUPT_001362"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHUPT_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0818999.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36398..36473)
                     /locus_tag="ACHUPT_001363"
     tRNA            complement(36398..36473)
                     /locus_tag="ACHUPT_001363"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACHUPT_001364"
     tRNA            complement(36482..36558)
                     /locus_tag="ACHUPT_001364"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8939 a        10012 c         9216 g         8420 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg tcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gatgtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAZOZX010000028        34929 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZOZX010000028 DAZOZX010000000
VERSION     DAZOZX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.028"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(147..1028)
                     /gene="prmA"
                     /locus_tag="ACHUPT_001365"
     CDS             complement(147..1028)
                     /gene="prmA"
                     /locus_tag="ACHUPT_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0819000.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1040..2491)
                     /gene="panF"
                     /locus_tag="ACHUPT_001366"
     CDS             complement(1040..2491)
                     /gene="panF"
                     /locus_tag="ACHUPT_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0819001.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2481..2723)
                     /locus_tag="ACHUPT_001367"
     CDS             complement(2481..2723)
                     /locus_tag="ACHUPT_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0819002.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2832..4181)
                     /gene="accC"
                     /locus_tag="ACHUPT_001368"
     CDS             complement(2832..4181)
                     /gene="accC"
                     /locus_tag="ACHUPT_001368"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0819003.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4192..4662)
                     /gene="accB"
                     /locus_tag="ACHUPT_001369"
     CDS             complement(4192..4662)
                     /gene="accB"
                     /locus_tag="ACHUPT_001369"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0819004.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAKSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5640..6614)
                     /locus_tag="ACHUPT_001370"
     CDS             complement(5640..6614)
                     /locus_tag="ACHUPT_001370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0819005.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6766..8706
                     /gene="csrD"
                     /locus_tag="ACHUPT_001371"
     CDS             6766..8706
                     /gene="csrD"
                     /locus_tag="ACHUPT_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0819006.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9011..10054
                     /gene="mreB"
                     /locus_tag="ACHUPT_001372"
     CDS             9011..10054
                     /gene="mreB"
                     /locus_tag="ACHUPT_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0819007.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10120..11223
                     /gene="mreC"
                     /locus_tag="ACHUPT_001373"
     CDS             10120..11223
                     /gene="mreC"
                     /locus_tag="ACHUPT_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0819008.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11223..11711
                     /gene="mreD"
                     /locus_tag="ACHUPT_001374"
     CDS             11223..11711
                     /gene="mreD"
                     /locus_tag="ACHUPT_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0819009.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11720..12313
                     /gene="yhdE"
                     /locus_tag="ACHUPT_001375"
     CDS             11720..12313
                     /gene="yhdE"
                     /locus_tag="ACHUPT_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0819010.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12303..13772
                     /gene="rng"
                     /locus_tag="ACHUPT_001376"
     CDS             12303..13772
                     /gene="rng"
                     /locus_tag="ACHUPT_001376"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0819011.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13840..17640
                     /gene="yhdP"
                     /locus_tag="ACHUPT_001377"
     CDS             13840..17640
                     /gene="yhdP"
                     /locus_tag="ACHUPT_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0819012.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17796..19241
                     /gene="tldD"
                     /locus_tag="ACHUPT_001378"
     CDS             17796..19241
                     /gene="tldD"
                     /locus_tag="ACHUPT_001378"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0819013.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19369..20298)
                     /gene="aaeR"
                     /locus_tag="ACHUPT_001379"
     CDS             complement(19369..20298)
                     /gene="aaeR"
                     /locus_tag="ACHUPT_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR0819014.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20481..20684
                     /gene="aaeX"
                     /locus_tag="ACHUPT_001380"
     CDS             20481..20684
                     /gene="aaeX"
                     /locus_tag="ACHUPT_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0819015.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20692..21624
                     /gene="aaeA"
                     /locus_tag="ACHUPT_001381"
     CDS             20692..21624
                     /gene="aaeA"
                     /locus_tag="ACHUPT_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0819016.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21630..23597
                     /gene="aaeB"
                     /locus_tag="ACHUPT_001382"
     CDS             21630..23597
                     /gene="aaeB"
                     /locus_tag="ACHUPT_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0819017.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTHFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23689..23961
                     /locus_tag="ACHUPT_001383"
     CDS             23689..23961
                     /locus_tag="ACHUPT_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0819018.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24017..24280)
                     /gene="yhcN"
                     /locus_tag="ACHUPT_001384"
     CDS             complement(24017..24280)
                     /gene="yhcN"
                     /locus_tag="ACHUPT_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0819019.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(24645..25115)
                     /gene="argR"
                     /locus_tag="ACHUPT_001385"
     CDS             complement(24645..25115)
                     /gene="argR"
                     /locus_tag="ACHUPT_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0819020.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            25550..26488
                     /gene="mdh"
                     /locus_tag="ACHUPT_001386"
     CDS             25550..26488
                     /gene="mdh"
                     /locus_tag="ACHUPT_001386"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0819021.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(26550..27617)
                     /gene="degS"
                     /locus_tag="ACHUPT_001387"
     CDS             complement(26550..27617)
                     /gene="degS"
                     /locus_tag="ACHUPT_001387"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0819022.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(27707..29054)
                     /gene="degQ"
                     /locus_tag="ACHUPT_001388"
                     /pseudo
     CDS             complement(27707..29054)
                     /gene="degQ"
                     /locus_tag="ACHUPT_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(29208..29606)
                     /gene="zapG"
                     /locus_tag="ACHUPT_001389"
     CDS             complement(29208..29606)
                     /gene="zapG"
                     /locus_tag="ACHUPT_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0819023.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            29800..30927
                     /gene="zapE"
                     /locus_tag="ACHUPT_001390"
     CDS             29800..30927
                     /gene="zapE"
                     /locus_tag="ACHUPT_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0819024.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            31146..31574
                     /gene="rplM"
                     /locus_tag="ACHUPT_001391"
     CDS             31146..31574
                     /gene="rplM"
                     /locus_tag="ACHUPT_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0819025.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            31590..31982
                     /gene="rpsI"
                     /locus_tag="ACHUPT_001392"
     CDS             31590..31982
                     /gene="rpsI"
                     /locus_tag="ACHUPT_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0819026.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            32377..33015
                     /gene="sspA"
                     /locus_tag="ACHUPT_001393"
     CDS             32377..33015
                     /gene="sspA"
                     /locus_tag="ACHUPT_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0819027.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            33021..33518
                     /gene="sspB"
                     /locus_tag="ACHUPT_001394"
     CDS             33021..33518
                     /gene="sspB"
                     /locus_tag="ACHUPT_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0819028.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(33561..34928)
                     /gene="dcuC"
                     /locus_tag="ACHUPT_001395"
     CDS             complement(33561..34928)
                     /gene="dcuC"
                     /locus_tag="ACHUPT_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0819029.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         8447 a         8759 c         9273 g         8450 t
ORIGIN      
        1 actcaaagaa atttgagtaa gttctcaatt tttctttatg aatgaaaatt ttggcacgca
       61 aatttgcgta aataatcggc aaatttacgt gccttttgtg agcttgctcg cacttcgccc
      121 cgcgtcaccc tacggcgatg cgaaggtcaa ttcttacgac cggtaatacg gcaccactct
      181 tctttttcca cgaccgggtc cagtgcgaag ctatcggcat aagcttcaca aacgctctct
      241 gcctggcttg ccagaatacc ggaaaggccc agcaaaccgc ctgaaactgg caggacgctg
      301 attaacggtg ccagttcacg taatgggcct gcaaggatgt tagcgaccac cacgtcggct
      361 ttcatttctt ctggctgatc tttcggtaag tagagttcca gacggtcaga aacgccatta
      421 cgttcggcgt tatcgcggct ggcctgaatc gcctgcggat cgatatcaat accaatggct
      481 tttgctgcac ccagtttcag cgccgcgatc gccagaatgc cggaaccaca gccaaagtcg
      541 atgactgttt taccggttaa atcgaggctg tcgagccatt gcaggcacag agaggtggtt
      601 ggatgggtac ccgtaccaaa cgccagccct ggatctaaca tcacgttgac ggcgttttcg
      661 tccggcacat cacgccagct agggcagatc cacagtcgtt caccaaagcg catcgggtgg
      721 aaattatcca tccattcgcg ctcccagtct ttatcttcta gttgttcgat tttatgcgcg
      781 aagcctgcgc cgagcagcgg atggttttcc agaatcgcca ccacgtcgtt catatcggtt
      841 tcagcgtcga acagaccaat cacatcggtg tcgccccaca ggcgcgtttc gcccggcagc
      901 ggctcaaata ctggcgtatc gtgggtatcc tgaaaagtga tagaaacggc acccgcttcc
      961 atcagcgcat cgctaagatc ttccgcgttc gcgccggtgg tgttcagttt cagttggatc
     1021 caaggcatgg caaaactctt tatttatcag tagtcaaaac ggtagcttgc gggacggatg
     1081 taccgaaacg gtttccgacc aggaaagcca gcaaacttag tagtaacgag ggcacgatag
     1141 ggtggaagcc caggtactga atattcagcg tcgcgagtac ggcatacagc acgccgccaa
     1201 cgatcatcgc acttagcgcg cctttggcgt tggcgcgttc ccagtaaaga cccagcacca
     1261 gcggccacag gaaaacggct tccagcccac cgaaggccag caaattcagc cagatgatca
     1321 tttctggcgg cttccaggcg gcaagcagca gcaacgcgcc gagaactaac gtaattaccg
     1381 ccgacatccg cttcagacgc gtctcgtttt gcatttgatc cggacggata ttcagataga
     1441 gatctttaat gatcgtagcg gaactttgca gcagttgggc gttaattgtc gacatgatcg
     1501 cagccatagg tgcagccaga aagatcccgg cagcaaaagg tggcagcact tttaccatta
     1561 acgtcgggat caccagatct ggtacggtga gatcggggat cactgcacga cctaacgctc
     1621 cggccaggtg cataccgaac atcaggattg cgactataat cgtaccgatg atgatccccc
     1681 ggtgtactgc tttgctgtct ttataagaga tacagcgcac cgcagtatgc ggcaggccaa
     1741 tcacgccaaa gcacaccagt acccagaacg acgtcataaa ggcaggcgac agaatatcgt
     1801 cagcgccttg tggcgtaacc agttgcggat cgatggtttg caaggtctgt actgcgttgc
     1861 ttaagccgcc agcggcatgt accacgccaa taagcagcac aacggtgcca atcagcatca
     1921 caagcccttg catggtgtcg ttcagcacgc tggcgcgaaa gccaccaaag gcggtatata
     1981 acgcaatgct gataccaaaa atcagcagcc cggtttcata aggaataccc gccgcggttt
     2041 ccagcaggcg cgcaccgccg ataaactgca cggtcatcgc accaacgaac gcaaccagca
     2101 aactcaaact cgccagccac accagaagac gactctggta gcgggcaaac agcatatcgt
     2161 tcagcgtcac tgcattgtag cggcgcgcaa gaatcgcaaa cttcttgccg agaataccga
     2221 gtgaaagcca gacagcagga agctgaatca tcgccagcaa tacccagccc agcccgtatt
     2281 tataagcagc tcctggcccg ccgataaacg aactggcact gatataggtc gcggtgagag
     2341 tcatcgccag cacaataccg cccatagagc ggctgccgag gaaatactca ttaaggaagg
     2401 tgccggtgct ccgtttacgc atcgcataaa ccgagatacc gaacaccacc accagatagg
     2461 cgaccagcgg tagaattact tcaagctgca tcgtcatcct ccagtgggat atcgcgatag
     2521 ataaatttca ccatcgccca gcacagtcca ataaacagta gcggcgtcag gatgcaggcc
     2581 atctcaaacc agcgcggaaa gccggtaaaa ccgggggcaa cgccaggtaa gtaagcgtct
     2641 actaaccaaa ctgccagata caaaagggtc agccccagcg cccagcgggc ctctttatgg
     2701 gcctgaacaa aacgagtgtc cattttttgt ccctgatggg tgaagaaagc gaggattgta
     2761 ccttatgggg gttgtcgatc cccagtaata aaaaaggccg gaaaatccgg ccttttgacg
     2821 cttttgcagt cttatttttc ctgaagaccg agttttttct ccagatagtg gatgttagtg
     2881 ccaccatgct ggaagttctc gtcattcatg atgcggatct gcagatcaac gttggttttg
     2941 ataccgtcga tgatcagctc ctgcagcgca ttcttcatgc gggcaatcgc cacgtcacgg
     3001 ttttcaccgt agcaaatcag cttaccgatc attgagtcat agtacggcgg tacggtgtag
     3061 cccgcgtaga tatgagactc ccaacgtacg ccaaaaccgc caggtgcgtg gaaacgggtg
     3121 attttgcctg ggcttggcag gaaggtgttc ggatcttcgg cgttgatacg acattccacc
     3181 gcatggccgc gaacgtgaac ttcttcttgc ttgatcgaca gcggttgacc ggcagcgata
     3241 cgcagctgtt ctttgatcag gtcaacgccg gtgatcattt ctgtaaccgg gtgttctacc
     3301 tgaatacggg tgttcatttc gatgaaatag aactcgccgt tttcgaacag gaactcgaaa
     3361 gtacctgcac cgcgatagcc gatatcaaca cacgctttag cgcaacgttc gccgatgtag
     3421 cgacgcagtt ccggggtaat gcccggtgct ggtgcttctt cgaccacttt ctggtggcgg
     3481 cgctgcatgg agcagtcacg ttccgccaga tagatagcgt tgccctgacc gtcagccagt
     3541 acctgaatct cgacgtggcg aggattctcc aggtatttct ccatgtaaac catatcgttg
     3601 ctgaaagcag ctttcgcttc cgcacgggtc atggagatgg attgtgccag ttcagcgtcg
     3661 ccgcgcacta cgcgcatacc gcgaccgccg ccgccgccgg aggctttgat aatcaccgga
     3721 taaccaatgc gtttagcaat ggcgcggttt ttatccatat cgtcgcccag cgggccgtca
     3781 gaaccaggta cgcaaggaac gcccgctttt ttcatcgccg cgattgcgga tactttgtcg
     3841 cccatcaggc gaatggtttc tgctttcggg ccgatgaaga taaagccgga gcgttcaacc
     3901 tgctcggcaa agttggcgtt ctcggagagg aagccgtaac ccggatggat tgctactgcg
     3961 ccggtgattt cagcggcgct gatgattgcc gggatgttca gataactttt tactgacgga
     4021 gcagggccaa tacagaccgt ttcatctgcc agtaatacgt gttttagatc gcgatccgtg
     4081 ctggagtgca cagcgacagt cttgatgccc agttctttac aggcacgaag aatacgcaat
     4141 gcaatctcgc cgcggttggc aataacaatt ttatccagca tgttcgcctc gttactcgat
     4201 gacgaccagc ggctcgtcaa attctaccgg ttgtccactt tcgaccagaa ttgctttcac
     4261 ggtaccggat ttgtccgctt cgatctggtt catcattttc atggcttcaa cgatgcacag
     4321 ggtatcgccc acgttgactt tctgacccac ttcgatgaac gcttttgcgt ccgggcttgg
     4381 ggtgcggtag aaagtaccaa ccatcgggga acgtacgatg tgaccactga tttccgctgc
     4441 tgctggcgct tccatggaag gaacggtcgc cggagcggct gcgttagatt tagctggctg
     4501 ctgcatcatt ggtgcagcgt aagcttgttg catcacaggg aaacttgcgg caggagctgc
     4561 acggctaatg cgtactgact cttcgccttc agaaatttcc agttcggaga tgcctgattc
     4621 ttcaaccagc tcgatcagtt ttttaatctt acgaatatcc atgagtgggt tccgtactct
     4681 ttgtttagtg tgattgtgac aggcgtttca ccgccgtctg taaagcgtat gccccgacag
     4741 ctgtatgcat agcgataaat tccagcaggc cgggacgccg gtctattttg ccccagagtg
     4801 tcaatgttag acttgacgga cattgtgcag cgatctgctg ctatcctccg gcaaaaaaca
     4861 aaatatacct tcgttgcgcc aatgtcacct tttccgcagc gcaaaaactg cgtcagggag
     4921 gacgaggtcg cacattataa cgatttcgta gcaattggca gctaaatact ggtcttatca
     4981 gggaatataa tcaacagcta acatgtaaat aaccttcaac accgtgtaat ttgcaacaag
     5041 ccgcacaatt cacgaaatta gcgccatcat cgacggaact tcttataacg taaggtgaaa
     5101 agctgaaaag ccagccctgc gaagatgagg ggctgcaatg agataatctt cacagaccac
     5161 aaataatgta gggatgccag gatcgcgaca agatagacga cattacgcag cggttgctgg
     5221 ggattaccca attttcactg catcgtctgg atagatgtga atgctaaagc acgcaaaatc
     5281 aaccaactga caattctcag cattaaatag gttcgagtgg ttaatttgta gtcaaataaa
     5341 atcagattat tcaccccatg gtaccagaaa tcagtaacag gttaagcgta gtgtcgccca
     5401 ggcaaagcac cataaaccta acaggcgacg gaggcgtatc aataatggct gtttagagga
     5461 gtatcaatgc gcatcaattt gtggtcagtc gaaatcatga ctttccagtt tggatgatgt
     5521 tgtacaactt cctgaccaag gtgttcgacg aaagctgtcc ggctaaccgc ttttcccgtt
     5581 aaccgattga ataatgatgt caaaatgagt tccctctctt attattcctt gctaaatggt
     5641 tagttaacct tcaccagcgt gcgaccctgg atctggttat taatgatggc ctcggcaaag
     5701 ttcggtgcct ctgacagaga tatctctttt gccgcctggg tatagaatga ttccggtaaa
     5761 tcggcgacca gtcgctgcca ggcttgtgcg cggcgttctg gtggcgtcat tactgaatcc
     5821 accccttgca aacggacatt acgcagaata aatggcatga ccgtggttgg cagagtaaaa
     5881 ccacccgcca gaccacaggc cgccacgcag ccgccgtaat tcatttgcgc cagcactttt
     5941 gccagcactt tgtcgccaac agtgtcaatt gccccagccc agacttgttt ttccagagga
     6001 cgggattcgg caaactcatc acgagggaga ataccgctgg cacctaaact tttcagatat
     6061 tcatgggtac tttcgcgacc ggaaacggca acgacctgat aacccaactt atgcagcagc
     6121 gccacggcgg tactgccgac gccaccactg gcacccgtca cgacaatctc accgtcctgc
     6181 gggcgaacac cggcatcttc cagcgccatc acacacagca tggcggtaaa accggcagta
     6241 ccgataatca ttgctttacg cgcgtccagc ccttgcggca tggcaaccag ccagtcacct
     6301 ttcactcgcg cctgctccgc cagcccaccc cagtggtttt caccaacgcc ccagccagtg
     6361 agtaacacct cctgaccggc atgaaaacgc ggatcttcgc tggtgcgtac agttccggca
     6421 aaatcgatcc caggaatcat cggaaaatta cggatgattt ttcccttacc ggtaatcgcc
     6481 agcgcatctt tatagttcag gctcgaccag tgaacatcga ccgtgacatc gccctccggc
     6541 aggcgacgtt cgtccagagt ctgtactgat gcgagagttt tgccgtcctg ctgttctaaa
     6601 agtaacgcct gcataagtgg tcctcatgtg catgatggat tggaaaataa tttctgaaga
     6661 ctatactcgc taatggaaaa gcaaagccga tgaagcgcaa taaattgcga gataaatctg
     6721 atttgctagt atgcccgctt cctcactatc ggagttaaca caaggatgag attaacgacg
     6781 aaattttcgg cctttgttac gctgctcacc gggttaacaa tttttgtgac tttgctgggc
     6841 tgttcgctaa gtttctacaa cgccattcag tataagttta gtcatcgcgt tcaggcggtg
     6901 gcgacggcga ttgataccca ccttgtgtcg aatgacttca gcgcattaag gccacaaatt
     6961 accgaattaa tggtgtcagc agatatcgtt cgtgtagacc tgctgcatgg tgataaacag
     7021 gtttataccc tggccagaaa tggtagttat cgtccggttg gcaccagcga tctgtttcgt
     7081 gaactgagcg ttccgttgat aaagcatccg gggatgtcgt tgcgtctggt ttatcaggat
     7141 ccgatgggca actatttcca ttcgttgatg accaccgcgc cgctcacggg ggcgattggc
     7201 tttatcattc ttatgctctt tctggcggta cgctggttac aacggcaact tgccgggcaa
     7261 gaattgctgg aaacccgggc tactcgtatc ttaaacggtg agcgtggctc taatgtgttg
     7321 ggaaccatct atgaatggcc gcccagaacc agcagtgcgc tggatacgct gcttcgtgaa
     7381 attcagaacg cacgcgaaca acacagccgt cttgatacgc tgatccgctc ttatgccgca
     7441 caggacgtga aaaccggcct caataaccga ctctttttcg ataatcagtt agcaacgtta
     7501 ctggaagatc aggagaaagt aggtacccac gggatcgtga tgatgattcg tctgccggat
     7561 ttcaatatgt tgagcgatac ctgggggcac agccaggttg aagaacagtt cttcactctg
     7621 acgaatctgc tgtcgacatt tatgatgcgc taccctggcg cactgctggc gcgttaccac
     7681 cgcagtgatt ttgctgcgct gttaccgcac cggacgttaa aagaggcaga gagcatagcc
     7741 agtcagttaa tcaaagccgt cgataccttg ccgaacaata aaatgctcga tcgcgacgat
     7801 atgatccaca ttggtatctg tgcctggcgt agtggtcagg ataccgagca ggtaatggaa
     7861 catgcagagt ctgccacgcg taatgcggga ttgcagggtg gcaatagctg ggctatttat
     7921 gatgactcgt tgcctgaaaa aggacgcggt aatgttcgct ggcgtacgct tatcgagcaa
     7981 atgctcagtc gcggcggccc gcgcctttat caaaaaccgg cggttactcg cgaaggtcag
     8041 gttcatcatc gcgaactcat gtgccgcatc ttcgatggta atgaagaggt tagctcggcg
     8101 gagtatatgc cgatggtctt gcagtttggc ttatcggaag agtatgaccg tctgcaaatc
     8161 agccgtctta ttccactatt gcgttactgg ccagaggaaa atctggcgat tcaggttacc
     8221 gttgagtcgc tgattcgtcc gcgttttcag cgttggctgc gcgatacgtt aatgcaatgt
     8281 gaaaaatcac aacgaaaacg cataattatt gaacttgcag aggccgatgt aggtcaacat
     8341 atcagtcgtt tacaacctgt tattcgttta gtgaatgctt taggggtacg ggtagccgtc
     8401 aaccaggctg gtttgacgct ggtaagcacc agttggatca aagaacttaa tgttgagtta
     8461 ctcaagctcc atccggggct ggtcaggaac attgagaagc gaacggagaa ccagctgctg
     8521 gttcaaagcc tggtggaagc ctgctccggg accagcaccc acgtttacgc caccggcgtg
     8581 cgttcgcgaa gcgagtggca gaccctgatt cagcgcggtg ttacaggcgg gcaaggggat
     8641 tttttcgcgt cctcacagcc acttgatact aacgtgaaaa aatattcaca aagatactcg
     8701 gtttaacctg ccgtttaatc cgttttcacg tagaataacg cgcgctgcgt ctcatgggag
     8761 tgtgcttgtc tgctcgccag attgttgcag cacatatgca gatgaatgac cttacgcggt
     8821 tgcaaacagg cgaggaatgc tgctgatgca ttaagccttt ctggactcag gcagagattt
     8881 gtaacaaagg aaacgaactg cactaatttt caccgtagca gatgattttt gcgccttgtc
     8941 gctgctgcgt gtggttggta aagtaagcgg attttctttt ccgccccagc tttcaggatt
     9001 atcccttagt atgttgaaaa aatttcgtgg catgttttcc aatgacttgt ccattgacct
     9061 gggtactgcg aataccctca tttatgtaaa aggacaaggc atcgtattga atgagccttc
     9121 cgtggtggcc attcgtcagg atcgtgccgg ttcaccgaaa agcgtagctg cagtaggtca
     9181 tgacgcgaag cagatgctgg gccgtacgcc gggcaatatt gctgccattc gcccaatgaa
     9241 agacggcgtt atcgccgact tcttcgtgac tgaaaaaatg ctccagcact tcatcaaaca
     9301 agtgcacagc aacagcttta tgcgtccaag cccgcgcgtt ctggtttgtg taccggttgg
     9361 cgcgacccag gttgaacgcc gcgcaattcg tgaatccgcg cagggcgctg gtgcccgtga
     9421 agtcttcctg attgaagaac cgatggctgc cgcaattggt gctggcctgc cggtttctga
     9481 agcgaccggt tctatggtgg ttgatatcgg tggtggtacc actgaagttg ctgttatctc
     9541 cttgaacggc gtggtttact cctcttccgt gcgcattggt ggtgatcgtt tcgacgaagc
     9601 tatcatcaac tatgtgcgtc gtaattacgg ttctctgatc ggtgaagcca ccgcagaacg
     9661 tatcaagcac gaaatcggtt cggcttaccc gggcgatgaa gtccgtgaaa tcgaagttcg
     9721 tggccgtaac ctggcagaag gtgttccacg cggttttacc ctgaactcca acgaaatcct
     9781 cgaagcactg caggaaccgc tgaccggtat tgtgagcgcg gtaatggttg cactggaaca
     9841 gtgcccgccg gaactggctt ccgacatctc cgagcgcggc atggtgctca ccggtggtgg
     9901 cgcactgctg cgtaaccttg accgtttgtt aatggaagaa accggcattc cagtcgttgt
     9961 tgctgaagac ccgctgacct gtgtggcgcg cggtggcggc aaagcgctgg aaatgatcga
    10021 catgcacggc ggcgacctgt tcagcgaaga gtaatcggat gcaggcaggg gaagcgtctg
    10081 tttaccctgc ctggtctgat acgagaatac gcataactta tgaagccaat ttttagccgt
    10141 ggcccgtcgc tacagattcg ccttattctg gcggtgctgg tggcgctcgg cattattatt
    10201 gccgacagcc gcctggggac gttcagtcaa atccgtactt atatggatac cgccgtcagt
    10261 cctttctact ttgtttccaa tgctcctcgt gaattgctgg atggcatatc gcagacgctg
    10321 gcctcgcgtg accaactaga acttgaaaac cgggcgttac gtcaggaact gttgctgaaa
    10381 aacagtgaac tgctgatgct tggacaatac aaacaggaga acgcgcgtct gcgcgagctg
    10441 ctgggttccc cgctgcgtca ggatgagcag aaaatggtga ctcaggttat ctccacggtt
    10501 aacgatcctt atagcgatca agttgttatc gataaaggta gcgttaatgg cgtttatgaa
    10561 ggccagccgg tcatcagcga caaaggtgtt gttggtcagg tggtggccgt cgctaaactg
    10621 accagtcgcg tgctgctgat ttgtgatgcg acccacgcgc tgccaatcca ggtgctgcgc
    10681 aacgatatcc gcgtaattgc agccggtaac ggttgtacgg atgatttgca gcttgagcat
    10741 ctgccagcga atacggatat tcgtgttggt gatgtgctgg tgacctccgg tctgggcggt
    10801 cgtttcccgg aaggctatcc ggtcgcggtt gtctcttccg taaaactcga tacccagcgc
    10861 gcttatactg tgatccaggc gcgtccgact gcaggactgc aacgtttgcg ttatctgctg
    10921 ctgctgtggg gcgcggatcg taacggcgct aacccgatga cgccggaaga ggtgcatcgt
    10981 gttgctaatg aacgtctgat gcagatgatg ccgcaggtat tgccttcgcc agacgcgatg
    11041 gggccaaagt tacctgaacc ggcaacgggg atcgctcagc cgactccgca gcaaccggcg
    11101 acaggaaatg cagctactgc gcctgttgcg ccgacacagc ccgctgctaa tcgctctcca
    11161 caaagggcta cgccgccgca aagtggtgct caaccgcctg cgcgtgcgcc gggagggcaa
    11221 tagtggcgag ctatcgtagc cagggacgct gggtaatctg gctctctttc ctcattgcgc
    11281 tgttgctgca aatcatgccc tggccggata acctgattgt tttccggcca aactgggtgt
    11341 tacttatctt gttgtattgg atcctggcct tgccccatcg cgtaaatgtg ggcacaggtt
    11401 ttgtgatggg tgccatactg gatctgatca gcggctcgat gcttggcgta cgcgtattgg
    11461 cgatgagcat cattgcttac ctggtggcgc tgaaatacca gcttttccgc aacctcgcat
    11521 tatggcagca ggcgctggtc gtcatgttgc tttcgctggt ggtggatatt attgttttct
    11581 gggcagagtt tttagtgatt aacgtctctt tcagaccgga agtgttctgg agtagtgtag
    11641 tcaatggggt gctctggccg tggattttct tgctgatgcg taaagtccgt cagcagtttg
    11701 cagtgcaata aaggtttcta tgacttctct gtatttagct tccggttctc cgcgtcgtca
    11761 ggagttactt gcgcaacttg gcgtgacctt tgaacgtatt gttacgggca ttgaggagca
    11821 gcgtcagccg caggagagcg cgcagcagta tgttgtgcgt ctggcacgcg agaaagcaca
    11881 ggcgggtgtc gcgcaaacgg cacaggatct cccggtgctg ggtgcggata ttatcgttat
    11941 cctgaacgga gaagtgctcg agaaaccgcg cgacgcagag catgcggcgc agatgttgcg
    12001 caaattatcg ggtcagaccc atcaggtgat gacggcagtg gcgttggccg acagccagca
    12061 cattctcgat tgcctggtgg tcaccgatgt gactttcaga acgttaacag acgaagacat
    12121 cgcgggctat gtcgccagcg gtgaaccgtt agataaagca ggtgcatacg gtattcaggg
    12181 gctgggtggc tgttttgtca ggaagataaa tggcagctat cacgccgtag tcggcttacc
    12241 gctggttgaa acgtatgaat tattaagtaa ttttaacgca ctgcgtgaga aaagggataa
    12301 acatgacggc tgaattgtta gtaaacgtaa cgccttcgga aacgcgagtg gcgtatattg
    12361 atggcggtat tctgcaggaa attcatattg aacgtgaggc gcgacgcgga atagtaggca
    12421 atatctacaa gggtcgtgta agtcgtgtac ttccgggtat gcaggcggct tttgtagata
    12481 ttgggctgga taaagccgcg tttcttcatg catccgacat catgccgcat accgaatgtg
    12541 tggcgggtga agaacaaaag caattcacgg tgcgcgacat ctcggaactg gttcgtcagg
    12601 ggcaagatct gatggtgcag gtggtgaaag atccgcttgg cactaaaggt gcgcgcctga
    12661 ccaccgatat cacgctgcct tctcgctatc tggtatttat gccaggggct tctcacgttg
    12721 gggtttccca acgaattgaa agcgaatcag aacgtgaacg tctgaaaaaa gtggtcgcag
    12781 agtattgcga cgagcagggc gggtttatca tccgcaccgc agcggaaggg gttggcgagg
    12841 ctgaactggc ctccgatgcc gcttatctga aacgcgtctg gaccaaagtt atggagcgta
    12901 aaaagcgccc gcagacccgt tatcagctgt acggcgaact ggcgctggcg cagcgtgttc
    12961 tgcgtgattt tgccgacgcc gaactggacc gcattcgcgt tgactcacgc ctgacttacg
    13021 aagcattgct ggagtttacc tcggagtaca ttcccgagat gaccagcaag ctggagcatt
    13081 acaccggacg ccagccgatt ttcgatctct ttgatgtcga aaacgaaatc cagcgagcgt
    13141 tggaacgcaa agtagaactg aaatccggtg gttatctgat tatcgaccag accgaagcga
    13201 tgaccaccgt ggacatcaat accggagcgt ttgtcggtca tcgcaatctg gacgacacta
    13261 ttttcaatac caatattgaa gcgacgcagg ctatcgctcg ccagttacgg ttgcgtaatc
    13321 tgggcgggat tatcattatt gatttcatcg atatgaataa tgaagatcac cgccgccgcg
    13381 tgctgcattc gctggagcag gcgttgagca aagaccgggt gaaaaccagc gttaatggtt
    13441 tttcggcgct ggggctggtg gagatgacgc gtaaacgcac ccgcgaaagc attgagcacg
    13501 tactgtgtaa cgaatgccca acctgccacg gtcgcggaac ggtgaaaacc gtggaaacgg
    13561 tatgctatga aatcatgcgc gagattgttc gtgtccacca tgcttacgac tccgaccgtt
    13621 tcctggtcta tgcttctccg gcagtagccg aagccttgaa aggcgaagag tcacactcgc
    13681 tggcggaagt ggaaattttc gttggcaaac aggttaaagt acaaattgaa ccgctctata
    13741 accaggagca gtttgacgtc gtaatgatgt aaacagatgc tgggccgcca tccggcaaag
    13801 ggtttttgag tcacattttt agcagacaag gagtgacggg tgaggcgatt gccggggatt
    13861 ttactgctta ctggagccgc gctcgttgtg atcgctgccc tgctggttag cggcctgcgt
    13921 attgctttac cgcatcttaa cgcctggcgt ccggaaatcc tcaacaaaat agaatccgcg
    13981 actggcatgc cggtagaagc cagtcagctc tcagccagct ggcagaattt tggcccgacg
    14041 cttgaagcac acgacatccg tgcagaacta aaagatggcg gcgaattttc ggttaaacgc
    14101 gttactctgg cgctggatgt ctggcagagc ctgttacata tgcgctggca gtttcgcgac
    14161 ctcactttct ggcagctgcg ctttcgcacc aacactccta tcaccagcgg tggtagcgat
    14221 gacagtctgg aagccagtca catcagcgat ctgtttcttc gtcaatttga ccatttcgat
    14281 ctccgcgaca gtgaagtcag ttttctgacg ccatccggtc agcgcgccga gctggcgatc
    14341 ccacaactca cctggctgaa cgatccacgt cgacaccgtg cggaaggcct ggtaagcctc
    14401 tccagcctta ccggacagca cggcgtgatg caggtgcgca tggatttgcg cgatgatgag
    14461 gggttgttaa gcaatggtcg cgtctggcta caggcggatg acatcgacct gaagccgtgg
    14521 ctcggtaaat ggatgcagga caatattgct ctggaaacgg cacagttctc ccttgaaggc
    14581 tggatgacga tcgacaaagg cgatgtaacc ggcggtgacg tctggctgaa acagggcggt
    14641 gccagctggt tgggcgagaa gcaaacgcat acgctgtcgg tggataatct gaccgcgcat
    14701 attacgcgtg aaaatccggg ctggcagttc tctattcccg atacacggat cacgatggac
    14761 ggcaaaccct ggccgagcgg agcattgacg ctggcctgga taccggaaca ggacgttggc
    14821 ggcaaagaca ataaacgcag tgacgaactc cggattcgcg ctagtaatct ggagctggca
    14881 ggcctggagg gcatacgccc gctggccgcg aaactttcac ctgcactggg tgatgtttgg
    14941 cgctccacac aaccgagcgg caagattaac actctggcgc tggatatccc gcttcaggcg
    15001 gcagacaaga cccgttttca ggcatcgtgg agcgatctgg cctggaagca atggaaatta
    15061 ttaccgggtg cggaacactt ctccgggacg ctttccggca gcgttgaaaa tggtttgctt
    15121 accgcgtcga tgaagcaggc aaagatgcct tacgaaacgg tattccgtgc gccactggaa
    15181 atcgccgacg gccaggcaac tataagctgg ctgaacaatg acaaaggttt ccagctggat
    15241 gggcgtaata ttgacgttaa agccaaagcc gtccatgcgc gcggcggttt tcgttacctg
    15301 caacctgcta acgatgaacc ctggctgggt attctggctg gcatcagtac cgatgatggt
    15361 tcacaagcct ggcgctattt cccggaaaac ttgatgggta aagacctggt tgattattta
    15421 agtggtgcga ttcagggcgg tgaagcggat aacgcgacgc tggtttatgg tggcaatccg
    15481 caactcttcc cctataaaca caacgaaggt cagtttgaag tgctggtgcc gctgcgcaac
    15541 gcgaagtttg ccttccagcc ggactggcct gcattaacta accttgatat tgaactggac
    15601 tttattaacg acggtttatg gatgaaaacc gatggcgtta atctgggcgg cgtgcgcgcg
    15661 agtaatctca ccgcagtgat ccctgactac tcaaaagaaa aactgctgat tgacgctgac
    15721 attaaaggtc cgggtaaagc cgttggccct tactttgatg agacaccgct gaaagattct
    15781 ctgggtgcga ccctgcaaga actccagctc gacggcgatg tgaatgctcg cttacatctt
    15841 gatatcccgc tgaacggcga actggtaacc gcgaaaggtg aagtgacgct gcgtaataac
    15901 agtctgttta tcaaaccact cgacagcatc ctgaaaaatt tgagcggtaa attcagcttt
    15961 atcaatggcg atctgcaaag tgaaccactg acagcaagct ggtttaatca gccgttgaac
    16021 gtggattttt ccaccaaaga aggggcaaaa gcctaccagg ttgcggtgaa tctcaacggt
    16081 aactggcaac cggcgaaaac cggcgttctg cctgaagcgg tgaacgaagc attgagtggc
    16141 agcgtggcgt gggatggtaa agtgggcatt gatctgcctt atcatgctgg cgcgacctat
    16201 aacgtagagc tgaacggcga tctgaataat gtgagtagtc acttaccttc accgttagcc
    16261 aaacctgcgg gtgaaccact agcggtaaac gttaaggttg atggcaatct caacagcttt
    16321 gaattaaccg gacaggctgg tgcggataac catttcaata gccgctggtt gcttggtcaa
    16381 aagctgacgc tcgatcgtgc tatttgggcg gcagacagta aaacgctccc gccgttgccg
    16441 gaacaaagtg gcgttgaact taatatgccg ccgatgaatg gtgccgagtg gctggccctg
    16501 ttccagaaag gcgctgcgga gagtgtcggt ggtgcagcga gtttcccaca acacataacg
    16561 ttacgtacgc ctatgttgtc actgggaaat cagcaatgga ataacctgag tattgtttcg
    16621 caaccgacgg caaatggcac cctggttgag gcgcaagggc gtgaaatcaa cgccacgcta
    16681 gcgatgcgta ataacgcgcc gtggctggcg aatatcaaat atctttatta caacccgagc
    16741 gtggcgaaaa ctcgtggtga ttcaacgccg tcatcacctt tcccgacaac ggagcgcatt
    16801 aacttccgtg gctggccgga cgcacaaata cgatgcacag agtgctggtt ctgggggcaa
    16861 aaattcggtc gcattgacag tgatatcacc atttctggcg atacgttaac gctgaccaat
    16921 ggactgattg atactggttt ctcgcggctt actgccgatg gtgaatgggt taataatccg
    16981 gggaatgaac gtacctcgct gaaaggaaaa ctgcgtgggc agaaaattga tgccgccgca
    17041 gaattttttg gtgtcacgac gcccatacgc cagtcgtcat ttaatgtgga ttacgattta
    17101 cactggcgca aagcaccctg gcagccagat gaagcgacgt tgaatggcat cattcatact
    17161 caactgggta aaggcgaaat taccgaaatc aataccggac atgccgggca attgctgcgc
    17221 ttattgagcg tagatgccct gatgcgtaag ctgcgttttg atttcagaga cacttttggc
    17281 gaagggttct attttgactc cattcgcagc accgcgtgga ttaaagacgg cgttatgcac
    17341 accgacgaca cgctggtgga tggcctggag gcggatatcg ccatgaaagg gtcggtaaat
    17401 ctggtacgcc gcgacctgaa tatggaagcg gttgtcgcac cagagatttc tgcgacggtg
    17461 ggcgtggctg cggcttttgc ggttaacccc attgttggcg cggcagtgtt tgccgccagt
    17521 aaagtgctgg ggccgctgtg gagcaaagtc tccattttgc gctatcacat ttcgggtccg
    17581 ctggacgatc cgcaaatcaa cgaagtgttg cgccaaccgc gtaaagaaaa agcgcaatga
    17641 tttgacgagg gcgcgtaatt gccccaatct catatgataa tcgttgccaa aggccaacga
    17701 gccggaacat aactgtaggc cggatagggc gttcacgccg catccggcag ccgtaaaaaa
    17761 tcctctactg cagtaactaa cgagtagcaa aaacgatgag tctgaacctg gtaagtgaac
    17821 aattgctagc ggcgaacggc ctgaaacatc aggacttgtt cgcgatcctc ggtcaactgg
    17881 ccgaacgtcg ccttgattat ggcgatctct attttcagtc gagctatcac gaatcctggg
    17941 ttttagaaga ccgcattatt aaagatggtt cttacaacat cgatcagggc gttggcgtgc
    18001 gtgcaatcag cggtgaaaaa accggatttg cttacgctga ccaaatcagc ctgctggcgc
    18061 tggaacagag tgcgcaagcg gcgcgcacca tcgtccgtga tagtggtgat ggcaaagtac
    18121 agacgctggg cgcggtagag catagcccgt tgtatacctc ggtagatccg ctgcaaagca
    18181 tgagccgtga agagaagctg gatatcctgc gtcgcgtcga taaggttgct cgcgaagcgg
    18241 acaagcgcgt acaggaagtg actgccagcc tcagtggtgt ctatgaatta attctggttg
    18301 cggccaccga cggcacgcta gcggcggatg tccgtccgct ggtgcgtctt tccgtgagcg
    18361 ttctggtcga agaagatggc aaacgcgaac gcggtgccag tggcggcggc ggtcgttttg
    18421 gttatgagtt cttccttgcc gatcttgacg gcgaagtccg tgcggatgca tgggcaaaag
    18481 aagcagtgcg tatggcgctg gtcaatcttt ctgccgttgc tgcaccagcg ggcaccatgc
    18541 cggtagtact tggcgcaggt tggccgggcg tgctgttgca tgaagcggta ggtcacggtc
    18601 tggaaggcga cttcaaccgc cgtggcactt cagtatttag tggacaggtc ggggagctgg
    18661 tggcttcaga actgtgtacc gtggttgatg acggcacgat ggtcgatcgc cgtggttcgg
    18721 tggcgattga tgatgaaggt acgccaggcc agtacaacgt gctgattgag aacggcattc
    18781 tgaaaggcta catgcaggat aaactcaacg cgcgtttgat ggggatgacg ccgactggca
    18841 acggtcgccg tgaatcctac gcccatctgc ccatgccgcg tatgaccaac acctatatgc
    18901 tgccgggtaa atcgaccccg caagaaatta ttgaatccgt tgagtacggt atctatgcac
    18961 cgaactttgg tggcggtcag gtggatatca cctccggcaa attcgttttc tccacttcag
    19021 aagcatatct gattgaaaac ggtaaagtaa cgaagccggt gaaaggcgca acgttgattg
    19081 gttccggtat cgaaaccatg cagcagattt cgatggttgg caacgacctg aaactggata
    19141 acggcgtggg tgtctgcggt aaagaagggc aaagtttgcc ggttggcgtg ggccagccaa
    19201 cgttgaaagt cgataacctg actgttggcg gtactgcgta ataatttcat tattttcaga
    19261 aggataatta atctttctac gtgcacgaac ggtcccctcg ctcctctggg gttagggtga
    19321 ggggaacccg ttggcacagg tttgtagaac gtaacagtac aatatgaatt acttctcttt
    19381 cccgcgcccg tgcatctcct gaaacaattt accgacctca acaaaataat ccgtcagcga
    19441 gttgatcacg acctgtacct tcagcggcag cttatctttt tcggtatata acgcataaac
    19501 cgggcgtgga tctgactgat aacgcggcag caggatctcc agctccccac gattgatctc
    19561 gttgatcacc cacatcagcg gcacgtaggc gatcccggca cccgccgtta gccagcgcac
    19621 cagcgtcatc ggatcattag tcacaaatct cccttgcggg atcaggcgag tcgagatccc
    19681 ttccggtgca atcagttcaa attcattgtc gggccgcacg ctgtattcaa gccatgaatg
    19741 gctacttaaa tcggcgggtt tttccggtat gccgtattgt gtgagatagc ttttcgcggc
    19801 gcacaccacc attggcatcg cgccaagacg gcgggaaaac aggctggaat cctgcaacgc
    19861 gccgacgcgg atcaccacat ccagaccgtc ggcaatcagg tcgggggctg gaattccggt
    19921 aaccagattg gcgctcaaac ctgggtattc tttcagcatt ttggctgtca gcccggcgag
    19981 aacattttgt gccatagttg aagaacagcc aatgcgtagc gtcccgatgg gggtgttatt
    20041 gaaggcatac agttgctcat gaacatcctg cacttcatga agcatacgac ggcagccctg
    20101 gtagtaaatt ctaccggctt cggtcaggcc aatgctgcgt gtgctacggt ttaacagctt
    20161 tacctgcaac tcatcttcca gttttgacac cgtctgactg atggacgaaa cgctcatctg
    20221 tagctgtctg gcggcggcgg taaaagagcc aaattcaact actttggcaa acaccgacat
    20281 gcgttttagt cgttccatta ttcactctga cttaaaagtg atttagatca cataatatag
    20341 ataacagcat aacagttacg ctaatatatt aaatatcaat ctacagcaat gttgctctcg
    20401 cccggctttc catgcccttc tctgcggcga cagatgctga aaataataac gcctgctctc
    20461 tctatacaac caaggtcaac atgagtctgt ttcccgttat cgtggtgttt gggctgtcct
    20521 tcccaccgat attttttgaa ttgcttttat cactggcgat tttctggctg gtgcgccggg
    20581 tacttgtgcc aacaggtatc tacgactttg tctggcatcc ggcgttgttc aacaccgcgc
    20641 tctattgctg cttgttttat ttgatatcgc gactgttcgt ttgaggttga agtgaaaaca
    20701 ctaataagaa aattctcccg tacggccatc acggtcgtat tagtcattct ggccttcatc
    20761 gcaattttta atgcctgggt ctattacacc gaatccccct ggacgcgtga cgcgcgcttt
    20821 agcgctgacg tcgttgcgat cgcgccggac gtttctggac tcattaccca ggtgaatgtt
    20881 catgataacc agctggtgaa aaaaggacag atactgttca ccatcgacca gccgcgctat
    20941 caaaaggcgc ttgaggaagc gcaagccgat gttgcttatt atcaggtact ggcacaggag
    21001 aaacgccagg aggccggacg tcgtaaccgt ctcggtgtgc aggcgatgtc tcgcgaagag
    21061 atcgaccagg ctaacaacgt actacaaacg gttctgcatc agttagcgaa agcgcaggcg
    21121 acccgcgatc tggcaaaact ggatcttgaa cgcacggtga tccgagcgcc agcagatggc
    21181 tgggtgacca acctcaacgt ctataccggt gagtttatta ctcgaggatc aacggcggtt
    21241 gcgctggtga aacagaactc cttctatgta ctggcctata tggaagaaac taagctggaa
    21301 ggggtgcgtc cggggtatcg tgcagagatc acgccgcttg gcagtaacaa agtgctgaaa
    21361 gggactgttg atagtgttgc cgctggggtc accaacgcca gcagcacgcg tgacgacaaa
    21421 gggatggcga ccatcgactc tgaccttgaa tgggtgcgtt tggcgcaacg tgttccggtt
    21481 cgtattcgtc tcgacaacca gcaagagaac atctggcctg cgggcaccac tgctacagtg
    21541 gtggtcactg gcaaacaaga tcgcgacgaa agccaggatt cgttcttccg taaaatggcc
    21601 catcgcctgc gtgagtttgg ttaatcacga tgggtatttt ctccattgct aaccaacata
    21661 ttcgctttgc ggtaaaactg gcgaccgcca ttgtactggc gctgtttgtt ggctttcact
    21721 tccagctgga aacgccacgc tgggcggtac tgacagcggc gattgttgcc gccggtccgg
    21781 cctttgctgc gggaggtgaa ccgtattctg gcgctattcg ctatcgtggc tttttgcgca
    21841 tcatcggcac atttattggc tgtattgccg gactggtgat catcattgcg atgatccgcg
    21901 caccattatt gatgattctg gtgtgctgta tctgggccgg tttttgtacc tggatatcct
    21961 cgctggtacg aatagaaaac tcgtacgcgt gggggctggc cggttatacc gcgctgatca
    22021 ttgtgatcac cattcagccg gaaccattgc ttacgccgca gtttgccgtc gaacgttgta
    22081 gcgagatcgt tatcggtatt gtgtgtgcga ttatggcgga tttgctcttt tctccgcgat
    22141 cgatcaaaca agaagtagat cgagagctgg aaagtttgct ggtcgcgcaa tatcaattaa
    22201 tgcaactctg tatcaagcat ggcgatggtg aagttgtcga taaagcctgg ggcgacctgg
    22261 tgcgacgcac cacggcacta caaggcatgc gcagcaacct gaatatggaa tcttcccgct
    22321 gggcgcgggc caatcgacgt ttaaaagcga tcaatacgct atcgctgacg ctgattaccc
    22381 aatcctgcga aacttatctt attcagaata cgcgcccgga attgatcact gatactttcc
    22441 gcgaattttt tgacacgccg gtagaaaccg cgcaggacgt ccacaagcag ctcaaacgcc
    22501 tgcggagagt tatcgcctgg actggggaac gggaaacgcc tgtcaccatt tatagctggg
    22561 tcgcggcggc aacgcgttat cagcttctca agcgcggcgt tatcagtaac acaaaaatca
    22621 acgccaccga agaagagatc ctgcaaggcg aaccggaagt aaaagtagag tcagccgaac
    22681 gtcatcatgc aatggttaac ttctggcgaa ccacactttc ctgcattctg ggcacgcatt
    22741 tctggctgtg gacgggctgg acttccggca gtggtgcaat ggtgatgatt gcggtcgtga
    22801 cgtcactggc aatgcgtttg ccgaatccac gcatggtggc gatcgacttt atctatggga
    22861 cgctggccgc gctgccgtta gggctgctct actttttggt gattatccct aatacccaac
    22921 agagcatgtt gctgctgtgt attagcctgg cagtgctggg attcttcctc ggtatagaag
    22981 tacagaaacg gcgactgggc tcgatggggg cactggccag caccataaat attatcgtgc
    23041 tggataaccc gatgactttc catttcagtc agtttctcga cagcgcatta ggacaaatcg
    23101 tcggctgtgt gctcgcgttc accgttattt tgctggtgcg ggataaatcg cgcgacagga
    23161 ctggacgtgt actgcttaat cagtttgttt ctgccgctgt ttccgcgatg actaccaatg
    23221 tggcacgtcg taaagagaac cacctcccgg cactttatca gcagctgttt ttgctgatga
    23281 ataagttccc aggggatttg ccgaaatttc gcctggcgct gacgatgatt atcgcgcacc
    23341 agcgcctgcg tgatgcgccg atcccggtta acgaggattt atcggcgttt caccgacaaa
    23401 tgcgccgcac agcagaccat gtgatatctg cccgtagcga tgataaacgt cgtcggtact
    23461 ttggccagtt gctggaagaa ctggaaatct accaggaaaa gctacgcatc tggcaagcgc
    23521 caccgcaggt gacggaaccg gttcatcggc tggcggggat gctccataag tatcaacatg
    23581 cgttgaccga tagttaagtc aaaaccgacg ccaaaagcgt cggttttttc atggctatac
    23641 ttagcgatgc acggcagaac tcgccgcgaa acgtgacggt ggcaacagat gaatatttat
    23701 gcctttgatt ttgatgagat tgagagtcag gaggattttt atcgtgactt tagccaaacc
    23761 tttggtctgg ctaaagataa ggtacgcgat ctcgactcac tatgggatgt gttaatgaac
    23821 gatgtcctgc cgctaccact tgagattgaa tttgttcatc tgggagagaa aacgcgtcgc
    23881 cgttttggcg cgttaatatt gctgtttgat gaggcagagg aagagctgga agggcatttg
    23941 cgttttaatg ttcgtcatta gcgcaaaaaa aagcccacga accgggggca atatcgtcgg
    24001 acaaaacgat gagggtttat ttgtacagtt cagccgtagc gtgccaggtg tcaccgctac
    24061 gagcttcagt aatctggtag gccgttgcgc ctttctcttc cgcttttttg ttcagcattt
    24121 cacgtatatc cattggcgaa gacgccacac cacttacgga tacggtcccg attgcttcac
    24181 gattttgtgc ttgtgcagca tcaatggagt cggcagcgaa tgcaccgaaa gagagaacag
    24241 aaagtacgct taatgcagct acagtggttt tgattttcat gatttttacc tcgacataat
    24301 cttttagctg ggcctttgtt tcgtgaccca tatcacaaaa tcaagtatac actaattatt
    24361 gaactaatta ataccacact aactgtttca gttgatacta agtgttaaaa atgtgaattt
    24421 taataacaaa aaagtataaa aaacacggtt atttattatt aattatagta ttttcaatta
    24481 gataatgaat tttgcataat gtgctttttt taccgtatgt attcattttg tgaatgatgt
    24541 gtcgcggtaa aacgcactat tcgttaaatg tatcgcagga gaaagaaggg gaatgagagg
    24601 gataagcaac attttccccg ccgccagaag cgacggggca gagattaaag ctcctggtcg
    24661 aacagctcta aaatcgcttc gtacaggtct ttgacggtga aaccgttagc aggggtggta
    24721 aagatggtgt catcgccagc gatggtgccc agaatacctt ctgctttgcc cagtgagtcc
    24781 agcaggcgag caattaactg cgccgcgcca gggctggtat gaatcacgac aactgcatcg
    24841 ttgtagtcga tatccagcac cagattcttc aatggactgg aggtggttgg tacacccagt
    24901 tcagctggca ggcagtaaac catttccatt ttggcattgc gtgtacgtac agcaccaaac
    24961 ttggtcaaca tccgcgagac tttagactga ttaatattgt caaagccttg ctcctgcaac
    25021 gcggcgacga tttcgccctg ggagctaaat ttctcttctt taagtaatgc tttaaatgct
    25081 ttaactagtt cttcttgctt agccgagctt cgcataagtc acccgatatg gtggttgata
    25141 caacattatt gtgcatacag atgaattttt atgcaaacag tcagccctga agaaggctga
    25201 aataatgtta tgaaagagcg ggattttatc aaatttcgtt attgagaaac atgcctgcgt
    25261 cacggcatgc aaattctgtt taaaagtaaa ttaattgtta tcaaattgat gttgttttgg
    25321 ctgaacggta gggtatattg tcaccacctg ttggaatgtt gcgctaatgc ataagcgact
    25381 gttaattacg taagttaggt tcctgattac ggcaattaaa tgcataaacg ctaaacttgc
    25441 gtgactacac attcttgaga tgtggtcatt gtaaacggca attttgtgga ttaaggtcgc
    25501 ggcagcggag caacatatct tagtttatca atataataag gagtttagga tgaaagtcgc
    25561 agtcctcggc gctgctggcg gtattggcca ggcgcttgca ctactgttaa aaacccaact
    25621 gccttcaggt tcagaactct ctctgtatga tatcgctcca gtgactcccg gtgtggccgt
    25681 cgatctgagc catatcccta ctgctgtgaa aatcaaaggt ttttctggtg aagatgcgac
    25741 tccggcgctg gaaggcgcag atgtcgttct tatctctgca ggcgtagcgc gtaaaccggg
    25801 tatggatcgt tccgacctgt ttaacgttaa cgccggcatc gtgaaaaacc tggtacagca
    25861 agttgcgaaa aactgcccga aagcgtgcat tggtattatc actaacccgg ttaacaccac
    25921 agttgcaatt gctgctgaag tgctgaaaaa agccggtgtt tatgacaaaa acaaactgtt
    25981 cggcgttacc acgctggata tcattcgttc caacaccttt gttgcggaac tgaaaggcaa
    26041 acagccaggc gaagttgaag tgccggttat tggcggtcac tctggtgtta ccattctgcc
    26101 gctgctgtca caggttcctg gcgttagttt taccgagcag gaagtggctg atctgaccaa
    26161 acgtatccag aacgcgggta ctgaggtggt tgaagcgaaa gccggtggcg ggtctgcaac
    26221 cctgtctatg ggccaggcag ctgcacgttt tggtctgtct ctggttcgtg cactgcaggg
    26281 cgaacaaggc gttgtcgaat gtgcctacgt tgaaggcgac ggtcagtacg cccgtttctt
    26341 ctctcaaccg ctgctgctgg gtaaaaacgg cgtggaagag cgtaaatcta tcggtaccct
    26401 gagcgcattt gaacagaacg cgctggaagg tatgctggat acgctcaaga aagatatcgc
    26461 cctgggcaaa gagttcgtta ataagtaatt gattagcgaa taataaaaaa ccggagcaca
    26521 gactccggtt tttgttttga gcactcgact taattggttg ccggatattc ctgaatggtg
    26581 acctgcagcg ttaactgctt atcatcacgc atcactacaa ccgggatcac cgaaccaggg
    26641 cgaatttccg ccacctgatc catcgtctcc agagcagaga tggccggttt gttatccacc
    26701 gaaataatca gatcgttgac ctgaataccc gcattcgccg ctgggccgtc aggtgacact
    26761 tcattaacca cgatcccttg cagttgatct ataccaccgc tctgcgcgtg cagtggtgcg
    26821 atctcgcgtc cgccgatacc aatgtagccg cggatcacgc gaccatcgcg gatcagctta
    26881 tccataattt tggttgctaa ctggaaagga atcgcaaagc cgataccttc cggcgtttcg
    26941 ccatcgttac tcttatcaaa cgacagcgtg ttaatgccca tcagttctcc cagcgagttc
    27001 accagcgcgc cgccagagtt accgtggtta atggaagcat cggtttgtag gaagttttgc
    27061 cgcccggtcg ggttcagacc gattcgaccc gtggcactaa taatcccttg ggtaatggtc
    27121 tgcccgaggt tgtacgggtt accgatcgcc agtactacgt cgccaatgtg cggtacgcga
    27181 cgtgcattaa ttggaatggt aggtaaaccg ccagtggcat taattttaag taccgccaga
    27241 tcggttagag agtcagatcc caccagcaat gcttcaaata cacgtccatc ctgtaaggcg
    27301 acgatgatct gatcggcgtc gttgatgacg tgtttattgg tgatgatata accgcgttga
    27361 tccatgatta caccggatcc cagggtgcgg atctcaagct ggttgtgaga gttggtgttc
    27421 aaaccacggt tgtaaacgtt aaccaccgct ggcgcggcgc ggcgaaccgc cagattatag
    27481 ctggcaggcg tctcatcggt actgtcaaat tgcggagtgg aaagcgggtt aaggctgcgc
    27541 agcgaaggca tggcaaccag cagaatagcg ccgacaatta atccaatcgc aacggaacgt
    27601 aagagcttca caaacatgat ggaggcgtca ttaaaaaagg gaacggcagc agcataccac
    27661 gagttaaccg gacatcacac gtcagcctga tgcccggttt acgacattaa cgcatcagca
    27721 gatagatgct ttcattgccg cgtacaattt gcagggcgat gatggccggt tttgccgcca
    27781 gcactttacg catttcagca atcgagttca cccgatcgcg gttgacgcca atgatcacat
    27841 cgtctttttg caagccagcc tgagcagctg ggcttccttt gacaacttca tctatcttaa
    27901 tacctttgcc gccatctttt agctgaccat cgctcaacgt tgcaccttcc agcgctggcc
    27961 gacgaagagg tgctggtatc aagcgtcact tctacttcca gtggtttgcc gttacgcagc
    28021 aggccaagct tcactttcgt gcccggctct gtagtcgcga tacgagagcg caactcagca
    28081 aagctattca gcggtttgcc gttgaggctg gtaataatat cgcccgcttt gacgcccgct
    28141 ttcgctgagc cagaacttgg caacacttcg ctgacaaacg cgccacgctg cacgtcaagg
    28201 ttgaaggctt tggcgatatc ggcactcatc tcggtgcctt tgatgcctaa caaaccgcgt
    28261 ttgatttcac caaagtcgat aagctgctgc gccagtgttc gcgccatatt actggggatg
    28321 gcaaatccaa tcccgacgct cccgccgcca ggcgcaagga ttgcagtgtt gatgccaatt
    28381 aactcaccgt taaggtttaa tagtgcaccg ccggagttac cgcggttaat ggaagcatct
    28441 gtctggataa agttttccag accttcaaga ttcaacccgc tgcggcctaa tgcggaaaca
    28501 atgccagagg tggcggtttg cccaaggcca aatgggttac cgaccgctac ggcaaaatca
    28561 ccgacgcgca atttatcgga gtcggcaata gcgatttgcg ttaatttgct cgggttttga
    28621 atttgtaaca gggcgatatc gctctggtca tcgctaccaa tcagttttgc atcaaactcg
    28681 cgcccatcat tgagctgaat actgattttc tgtgcctgat taatcacatg gttgttggtc
    28741 agcacatagc ctttactggc gttgatgatg acaccggaac ctaaaccttc gaagggttgt
    28801 gcaggttgat ccggtaaatc atcaccaaaa aactttttga attcttccgg gattttctgt
    28861 ccctgactgg ccgttccttc cacccgtacg ctcaccactg ccggaagcac tttttccagc
    28921 attggagcca gactggggag aggggcctga tcggcaacct ggcctggaat cgacgcgacg
    28981 gcctgaaatg acgccgagag agttaacccg gcacttaacg ctaatgcact caacagctgg
    29041 gtttgttttt tcattattcc tgctctcgta cctgaatgat aagaaaagag attcaaaaca
    29101 gtttgatgtt attgaatttt cagacggcta acaatgagac accagattaa atgatagtcg
    29161 gggagggaga gaagaggagg gcgcaatggc tgcgcccgaa aaataaatta gtcgcgcttc
    29221 gcgccagtac gcagcaggcc ggatgcgcct tcagaatagt cgcgaggcac ctgcaccggt
    29281 gcctgatcgt tgctggcttc agactctgcc agacgattac ggaacgggtt tgcttcagca
    29341 gacagttccg gcagcaggct gctggagctt tttgccatgt gctgatacag ctggcgatag
    29401 tcgtgcgcca tggtatccag taattccgcg ctgcgggcaa agtggctaac cagctcttcg
    29461 cgatactcgt ccagttcagc tttattcttt tccagttcgt actgcaacgc ctgttgctgg
    29521 cgtagtttac gattaccaaa acgcatggcc acagcaccaa taatgatgcc gacgactaac
    29581 ccaattagcg catattccca ggtcatgaac atctcccgtt gtcttttgtt tccgtagggt
    29641 gttggcttca ggctccatgc ctgcggctga ttatgccact ataaccgtta attccacaga
    29701 agtggaatcc cgactgcata tcgcgtagtg tagaacggcc tttttttcgt caaccgtgaa
    29761 caacggcgca ccgattattc aaggaataac aataagatca tgcaaagcgt taccccaaca
    29821 tcgcaatacc tgaaggcgct caatgaaggc agccatcaac ccgacgacgt tcaaaaagag
    29881 gccgtcagcc gcctggaaat tatttatcaa gaactcatca atagcaggcc accagccccc
    29941 aggacgagtg ggctaatggc gcgggtcggt aagctgtggg gtaaacgcga agacacaaag
    30001 catacgccag tgcgtggctt atatatgtgg ggcggtgtag gacgcgggaa aacctggctg
    30061 atggaccttt tctatcaaag cctgccggga gagcggaaac agcgcctgca ctttcaccgt
    30121 tttatgctgc gggtgcacga agagctaact gccttacagg ggcagaccga tccgctggaa
    30181 attattgccg atcgctttaa agccgaaact gacgtgctct gttttgacga attttttgtt
    30241 tctgatatta ccgacgccat gctacttggc ggtctgatga aagccctgtt cacccgcggt
    30301 attaccctgg tagcgacgtc aaatattccg ccggacgaac tttatcgaaa tggcctgcaa
    30361 cgtgcgcgtt ttctgcctgc aatcgatgcc attaaacagc attgtgatgt aatgaacgtg
    30421 gacgctggtg ttgattatcg actgcgtaca ctcactcagg cgcatctgtg gctttcgcca
    30481 ctcaacgatg aaacccgggc gcagatggat aaactatggt tggcgctggc gggggtgaaa
    30541 cgagaaaatt caccgacgtt agaaatcaac catcggccat tggcgacaat gggcgtcgag
    30601 aaccagacgc tggcggtttc ttttactacg ctgtgcgtcg acgcccgcag tcagcatgac
    30661 tatattgcgc tctcacgcct gtttcacaca gtaatgttgt ttgatgtgcc agtcatgacg
    30721 cggttgatgg agagcgaagc gcggcgcttt attgcgctgg tggatgagtt ttacgagcgc
    30781 catgtcaaat tagtggtgag tgcagaagtg ccgctgtatg aaatttatca gggtgagcgg
    30841 ctgaagtttg agttccagcg ttgcctgtca cgtctgcaag agatgcaaag cgaagagtat
    30901 ctgaagcgcg agcatttggc gggttaaaac ctgccacaaa tcacaaaaag gggtcgatct
    30961 ttgaccccga cttctctata atcctgcgac cccacgttac aagaaagttt ttttcccaaa
    31021 actttttgtg tgctggcata ggctattcga aggggtaggt ttgccggact ttgtcgtgtg
    31081 aacctcaaca attgaagacg tttgggtgtt caccaacgtg taactattta ttgggtaagc
    31141 ttttaatgaa aacttttaca gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg
    31201 acgcgaccgg taaaactctg ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta
    31261 agcacaaagc ggaatatact ccgcacgtag ataccggtga ttacatcatc gttctgaacg
    31321 ctgacaaagt tgctgtaacc ggcaacaagc gtactgacaa agtgtactat caccacaccg
    31381 gccacatcgg tggtatcaaa caagcgacct ttgaagagat gattgctcgc cgtcctgagc
    31441 gtgtgattga aatcgcggtt aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt
    31501 tccgtaaact gaaagtttac gcgggtaacg agcacaacca cgcggcacag caaccgcaag
    31561 ttcttgacat ctaatcggga ttataggcaa tggctgaaaa tcaatactac ggcactggtc
    31621 gccgcaaaag ttccgcagct cgcgttttca tcaaaccggg caacggtaaa atcgtaatca
    31681 accaacgttc tctggaacag tacttcggtc gtgaaactgc ccgcatggta gttcgtcagc
    31741 cgctggaact ggtcgacatg gttgagaaac tggacctgta catcaccgtt aaaggtggtg
    31801 gtatctctgg tcaggctggt gcgatccgtc acggtatcac ccgcgctctg atggaatacg
    31861 acgagtccct gcgttctgaa ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg
    31921 ttgaacgtaa gaaagtcggt ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt
    31981 aattggcttc tgctacggca gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt
    32041 tatggctaaa ttctgaatca gcgtaaaaaa tgggaagttg ctttttgctg ccgtctgaaa
    32101 gacaggtaaa acaaaaacca catcgccaat aagggtctaa gtcaactatt tcagactaaa
    32161 gcgcatctct ttttccccat ttccggcatc cactcaccac aaaggtcgca aaatctggta
    32221 aactatcatc caattttctg cccaaatgtc gggtattgct cattttttgt ttgattttcg
    32281 aacaaagaga gtcgttcctt attgggtaac acaacttctg actggccacc tggtggctgg
    32341 tagcagtaaa aattctgact atacctggag gttttcatgg ctgtcgctgc caacaaacgt
    32401 tcggtaatga cgctgttttc cggtcctact gacatctata gccatcaggt ccgcattgtg
    32461 ctggctgaga aaggtgtaag tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag
    32521 gatctgattg acctcaaccc gaatcagagc gttccgaccc tggtggatcg tgagctgacc
    32581 ctgtgggaat ctcgcatcat tatggaatat ctggatgagc gtttcccgca tccgccactg
    32641 atgcctgttt acccggtagc tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa
    32701 gactggtaca cgctgatgaa cactatcatc aacggttcag cttctgaagc agatgccgca
    32761 cgtaagcaac tgcgcgaaga actgctggcg attgcgccgg tcttcggtca gaagccgtac
    32821 ttcctgagcg atgagttcag cctggtcgat tgctaccttg ctccgctgct gtggcgtctg
    32881 ccgcaactgg gcatcgagtt cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc
    32941 cgcgtctttg agcgtgactc tttccttgct tctttaactg aagcagaacg tgaaatgcgt
    33001 ctgggccgga gttaatctgt atggatttgt cacagctaac accacgtcgt ccctatctgc
    33061 tgcgtgcatt ctatgagtgg ttgctggata accagctcac gccgcacctg gtggtggatg
    33121 tgacgctccc tggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca
    33181 acattgcgcc gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggtg cgctttaacg
    33241 cgcgctttgg tggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct
    33301 acgcccgtga aaatggcgca ggcacgatgt ttgagcctga agctgcctac gatgaagata
    33361 ccagcatcat gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttattg
    33421 atggcgacaa gccagatcac gatgatgaca ctcatcctga cgatgaacct ccgcagccac
    33481 cacgcggtgg tcgaccggca ttacgcgttg tgaagtaata caaaacaggc ccaggcggcc
    33541 tgttttgtct ttttaatgac ttaataaaat agcgtgattg tggcaatcat attcaccacg
    33601 aaaccgactg ccatggggat cgctgtccgc ttcaccaccg caaaaggcga aacgcctgca
    33661 attcctgacg taacgaccac caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg
    33721 gcaaaatgca tcggcataat cattacaacc gctggtacat gtagtccggc tgcgatattc
    33781 ggaataagac tggcaaatga cataaacggc gcattgccag agcccatcac aatggcacaa
    33841 atggcaatga ctagcgccat aataatcatc acgccaatac cgcccagacc agaatgctcc
    33901 gcacccctga taaccgcatc aacagtgcca atcgtcgtta agcctttcgc aaatatttca
    33961 cccgcgacca ccagcgttac cacgttgaca aactgcgtac ccatgccgtc aaaaaacgcc
    34021 tgcacatcgt ccattgtttc gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc
    34081 accgtcaaac ttagtaacat caccaccacc agatgcagtt ctgactgcat cagcccgacg
    34141 tgggcgagga acagcgagcc gagcatcagg attaacggca tcacaggtaa aatggcgtaa
    34201 tagagcggcg ggacattatc gagagttttt tgctctgcct gttcgtgatt gatattttta
    34261 tcttttttgt caaaagcgcg ttgcacgaaa aagtgggaga tcgccaccga gataatgacg
    34321 caagaggcga ccggaagctg gtagtggaag aagtatgtgg caattttcat tcccgcgacc
    34381 tgggctgcaa aaatggagtt cgtttccaga atcccccact caatggacat cgtggttgcg
    34441 ataaccgcca ccgcagagag gcgacttact cccagactca ccagcgtcgg aaataaggtg
    34501 accatcagca acatacccag accggaggcg ctggtaataa actgcgccat gatttggccg
    34561 atgacgtaag ttgccgacag aataatatac ggcgagcgaa tgagttttaa cgggcggctt
    34621 agcaagctca ccatcgcgca actggctcct atgcgctcca tgtagcgggc ataaccgccc
    34681 accgccataa tcgacagccc caatccggca atgcggttgg acagcatgcg caggatttca
    34741 ttataaatgt cgacgatcaa gtagccgctg cttttggcgg gatcgagtac accaccgaac
    34801 cctaaccaga ctccgcacat catcaggaag acacctgcgg cagccagcac cacctgaggt
    34861 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg
    34921 ccgaacatt
//
LOCUS       DAZOZX010000029        34499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZOZX010000029 DAZOZX010000000
VERSION     DAZOZX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.029"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(183..326)
                     /gene="ypaB"
                     /locus_tag="ACHUPT_001396"
     CDS             complement(183..326)
                     /gene="ypaB"
                     /locus_tag="ACHUPT_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0819030.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            454..2739
                     /gene="nrdA"
                     /locus_tag="ACHUPT_001397"
     CDS             454..2739
                     /gene="nrdA"
                     /locus_tag="ACHUPT_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0819031.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPNIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2973..4103
                     /gene="nrdB"
                     /locus_tag="ACHUPT_001398"
     CDS             2973..4103
                     /gene="nrdB"
                     /locus_tag="ACHUPT_001398"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0819032.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4103..4357
                     /gene="yfaE"
                     /locus_tag="ACHUPT_001399"
     CDS             4103..4357
                     /gene="yfaE"
                     /locus_tag="ACHUPT_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0819033.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4411..5061)
                     /gene="inaA"
                     /locus_tag="ACHUPT_001400"
     CDS             complement(4411..5061)
                     /gene="inaA"
                     /locus_tag="ACHUPT_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR0819034.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5141..5245)
                     /locus_tag="ACHUPT_001401"
                     /pseudo
     CDS             complement(5141..5245)
                     /locus_tag="ACHUPT_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5240..5482
                     /locus_tag="ACHUPT_001402"
                     /pseudo
     CDS             5240..5482
                     /locus_tag="ACHUPT_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5524..6600)
                     /gene="glpQ"
                     /locus_tag="ACHUPT_001403"
     CDS             complement(5524..6600)
                     /gene="glpQ"
                     /locus_tag="ACHUPT_001403"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0819035.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6605..7963)
                     /gene="glpT"
                     /locus_tag="ACHUPT_001404"
     CDS             complement(6605..7963)
                     /gene="glpT"
                     /locus_tag="ACHUPT_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0819036.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8236..9864
                     /gene="glpA"
                     /locus_tag="ACHUPT_001405"
     CDS             8236..9864
                     /gene="glpA"
                     /locus_tag="ACHUPT_001405"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0819037.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9854..11113
                     /gene="glpB"
                     /locus_tag="ACHUPT_001406"
     CDS             9854..11113
                     /gene="glpB"
                     /locus_tag="ACHUPT_001406"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0819038.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11110..12300
                     /gene="glpC"
                     /locus_tag="ACHUPT_001407"
     CDS             11110..12300
                     /gene="glpC"
                     /locus_tag="ACHUPT_001407"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0819039.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12493..13443
                     /locus_tag="ACHUPT_001408"
     CDS             12493..13443
                     /locus_tag="ACHUPT_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0819040.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13484..13945)
                     /locus_tag="ACHUPT_001409"
                     /pseudo
     CDS             complement(13484..13945)
                     /locus_tag="ACHUPT_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(14009..15211)
                     /locus_tag="ACHUPT_001410"
     CDS             complement(14009..15211)
                     /locus_tag="ACHUPT_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0819041.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15298..15840)
                     /locus_tag="ACHUPT_001411"
     CDS             complement(15298..15840)
                     /locus_tag="ACHUPT_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0819042.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16119..16544
                     /gene="nudI"
                     /locus_tag="ACHUPT_001412"
     CDS             16119..16544
                     /gene="nudI"
                     /locus_tag="ACHUPT_001412"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0819043.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16583..17185)
                     /gene="ais"
                     /locus_tag="ACHUPT_001413"
     CDS             complement(16583..17185)
                     /gene="ais"
                     /locus_tag="ACHUPT_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0819044.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17493..18632
                     /gene="arnB"
                     /locus_tag="ACHUPT_001414"
     CDS             17493..18632
                     /gene="arnB"
                     /locus_tag="ACHUPT_001414"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0819045.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18636..19604
                     /gene="arnC"
                     /locus_tag="ACHUPT_001415"
     CDS             18636..19604
                     /gene="arnC"
                     /locus_tag="ACHUPT_001415"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0819046.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19604..21585
                     /gene="arnA"
                     /locus_tag="ACHUPT_001416"
                     /pseudo
     CDS             19604..21585
                     /gene="arnA"
                     /locus_tag="ACHUPT_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21582..22472
                     /gene="arnD"
                     /locus_tag="ACHUPT_001417"
     CDS             21582..22472
                     /gene="arnD"
                     /locus_tag="ACHUPT_001417"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0819047.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVECCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22472..24124
                     /gene="arnT"
                     /locus_tag="ACHUPT_001418"
     CDS             22472..24124
                     /gene="arnT"
                     /locus_tag="ACHUPT_001418"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0819048.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24121..24456
                     /gene="arnE"
                     /locus_tag="ACHUPT_001419"
     CDS             24121..24456
                     /gene="arnE"
                     /locus_tag="ACHUPT_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0819049.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24456..24842
                     /gene="arnF"
                     /locus_tag="ACHUPT_001420"
     CDS             24456..24842
                     /gene="arnF"
                     /locus_tag="ACHUPT_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0819050.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24836..25108)
                     /gene="pmrD"
                     /locus_tag="ACHUPT_001421"
     CDS             complement(24836..25108)
                     /gene="pmrD"
                     /locus_tag="ACHUPT_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0819051.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25213..26568)
                     /gene="menE"
                     /locus_tag="ACHUPT_001422"
     CDS             complement(25213..26568)
                     /gene="menE"
                     /locus_tag="ACHUPT_001422"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0819052.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26565..27527)
                     /gene="menC"
                     /locus_tag="ACHUPT_001423"
     CDS             complement(26565..27527)
                     /gene="menC"
                     /locus_tag="ACHUPT_001423"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0819053.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27527..28384)
                     /gene="menB"
                     /locus_tag="ACHUPT_001424"
     CDS             complement(27527..28384)
                     /gene="menB"
                     /locus_tag="ACHUPT_001424"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0819054.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28399..29157)
                     /gene="menH"
                     /locus_tag="ACHUPT_001425"
     CDS             complement(28399..29157)
                     /gene="menH"
                     /locus_tag="ACHUPT_001425"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0819055.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29154..30824)
                     /gene="menD"
                     /locus_tag="ACHUPT_001426"
     CDS             complement(29154..30824)
                     /gene="menD"
                     /locus_tag="ACHUPT_001426"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0819056.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30913..32208)
                     /gene="menF"
                     /locus_tag="ACHUPT_001427"
     CDS             complement(30913..32208)
                     /gene="menF"
                     /locus_tag="ACHUPT_001427"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0819057.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32287..32592)
                     /gene="elaB"
                     /locus_tag="ACHUPT_001428"
     CDS             complement(32287..32592)
                     /gene="elaB"
                     /locus_tag="ACHUPT_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0819058.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32647..33108)
                     /locus_tag="ACHUPT_001429"
     CDS             complement(32647..33108)
                     /locus_tag="ACHUPT_001429"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0819059.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33173..34090
                     /gene="rbn"
                     /locus_tag="ACHUPT_001430"
     CDS             33173..34090
                     /gene="rbn"
                     /locus_tag="ACHUPT_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0819060.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34281..34469
                     /locus_tag="ACHUPT_001431"
                     /pseudo
     CDS             34281..34469
                     /locus_tag="ACHUPT_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8402 a         9270 c         8576 g         8251 t
ORIGIN      
        1 tttaacaata accgaataga aaacaaccat ttcgacatca acaatcgctt acattcgctt
       61 atatattgac cacaactgat acatcagatt atgtgatgac tcgtgcttag atcaattttt
      121 gcaatcatta gcaaaaagat taataaacca tctatatcaa tttatccaac ctattctgcc
      181 gttcaagaaa tcgccgaaca gttattttta acaaattttt ctcttcccat tgactttccc
      241 ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga gttatccaca
      301 aagttatgca cttgcaagag ggtcattttc acactatctt gcagtgaatc ccaaacatac
      361 cccctatata tagtgttcta agcagcttcc cttactacag gtagtctgca tgaaactatt
      421 gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct ggtgacaaag
      481 cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct ggattgggcg
      541 gcagaaggac tgcataacgt ttcgatttcc caggtcgagc tgcgctccca cattcagttt
      601 tatgacggta tcaagacctc tgacatccac gaaactatta tcaaggctgc cgcagacctg
      661 atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcgat cttccacctg
      721 cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccacgt ggtgaaaatg
      781 gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga agagttcaag
      841 cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc tgccgttaag
      901 cagctggaag gcaaatatct agtacagaac cgcgtgaccg gcgaaatcta cgaaagcgcc
      961 cagttccttt atattcttgt tgccgcgtgc ctgttctcga actacccgcg tgaaacgcgc
     1021 ctgcaatacg tgaagcgttt ttatgacgcg gtttccacat ttaaaatttc gctgccgacg
     1081 ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt actgatcgag
     1141 tgcggtgaca gcctggattc catcaacgcc acctccagcg cgattgtgaa atatgtttcc
     1201 cagcgtgccg ggatcggcat caacgccgga cgtattcgtg cgctgggcag cccgattcgc
     1261 ggtggcgaag cgttccatac cggctgtatc ccgttctaca aacatttcca gacagcggtg
     1321 aaatcctgct ctcagggcgg tgtgcgcggc ggtgcggcaa cgctgttcta cccgatgtgg
     1381 catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggggttga aggcaaccgc
     1441 gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg cctgctgaaa
     1501 ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga cgcgttcttc
     1561 gccgatcagg aagagtttga acgcctgtat accaaatatg agaaagacga cagcatccgc
     1621 aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg tgcgtctacc
     1681 ggtcgtatct atattcagaa cgttgaccac tgtaataccc atagtccgtt tgatccgaac
     1741 atcgcgccag ttcgccagtc taacctgtgc ctagaaattg ccctgccgac caaaccgctg
     1801 aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc tttcaacctg
     1861 ggcgcaatta atagcctgga tgaactggaa gagctggcga ttctggcggt tcgtgcactt
     1921 gacgcgctac tggattatca ggattacccg atcccggccg ccaaacgtgg agcgatgggg
     1981 cgtcgtacgc tgggtattgg tgtgattaac ttcgcttact atctggcgaa gcacggtaaa
     2041 cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc gattcagtat
     2101 tacctgctga aagcgtctaa tgagctggct aaagagcaag gcgcgtgccc gtggtttaac
     2161 gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga tctggatgcc
     2221 atcgctaatg agccgctgca ttacgactgg gaagcattgc gtgaatcaat caaaacgcac
     2281 ggcctgcgta actccacgct ttctgccctg atgccgtccg agacctcttc gcagatctct
     2341 aacgccacta acggtattga accgccgcgc ggttacgtca gcatcaaagc gtcgaaagac
     2401 ggtattttgc gccaggtggt gccggactac gagcacctgc acgacgccta tgagctgctg
     2461 tgggaaatgc cgggtaacga tggctacctg caactggtag gtatcatgca gaaatttatc
     2521 gatcagtcga tctctgctaa caccaactac gatccgtcac gtttcccgtc aggaaaagtg
     2581 ccgatgcagc agttgctgaa agacctgctc accgcctaca aattcggggt caaaacgctg
     2641 tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt accgtcaatc
     2701 caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc cggatgcggc
     2761 gtaaacgcct tatccggcct acggctcggt ttgtaggcct gataagacgc gccagcgtcg
     2821 catcaggctc cgggtgccgg atgcagcgtg aacgccttat ccggcctacg gctcggattt
     2881 gtaggcctga taagacgcgc cagcgtcgca tcaggcacag gatgcggcgt aaaatgcctt
     2941 atccggcatt aaactcccaa caggacacac tcatggcata taccaccttt tcacagacga
     3001 aaaatgatca gctcaaagaa ccgatgttct ttggtcagcc ggtcaacgtg gctcgctacg
     3061 atcagcaaaa atatgacatc ttcgaaaagc tgatcgaaaa gcagctctct ttcttctggc
     3121 gtccggaaga agttgacgtc tcccgcgacc gtatagatta ccaggcgctg ccggagcacg
     3181 aaaaacacat ctttatcagc aacctgaaat atcagacgct gctggattcc attcagggtc
     3241 gtagcccgaa cgtggcgcta ttgccgctta tttctattcc ggaactggaa acctgggtcg
     3301 aaacctgggc gttctcagaa acgattcatt cccgttccta tactcatatc attcgtaata
     3361 tcgttaacga tccgtctgtt gtgtttgacg atatcgtcac caacgagcag atccagaaac
     3421 gtgcggaagg gatctccagc tattacgatg aactgatcga aatgaccagc tactggcacc
     3481 ttctgggcga aggcacccac accgttaacg gtaaaaccgt aaccgttagc ctgcgcgagc
     3541 tgaagaaaaa actgtatctc tgcctgatga gcgttaacgc gctggaagcg atccgcttct
     3601 acgtcagctt tgcttgttcc tttgcctttg cagaacgcga actgatggaa ggtaacgcca
     3661 agattattcg cctgattgcc cgcgatgaag ccctgcacct gaccggtact cagcatatgc
     3721 tgaatctgct gcgcagcggc gcggatgatc cggagatggc ggaaattgcc gaagaatgta
     3781 agcaggagtg ctatgacctg tttgtccagg cggcacagca agagaaagac tgggcggatt
     3841 atctgttccg tgacggttcg atgattggtc tgaataaaga tattctctgc cagtacgttg
     3901 aatacatcac caatatccgc atgcaggcag tcggtttgga tctgccgttc cagacgcgct
     3961 ccaacccgat cccgtggatc aacacctggc tggtgtctga taacgtgcag gttgctccgc
     4021 aggaagtgga agtcagttct tatctggtcg ggcagattga cgcggaagtg gacaccgacg
     4081 atttgagtaa cttccagctc tgatggcccg cgttaccctg cgcatcactg gcacacaact
     4141 gctgtgccag gatgaacacc cttcccttct ggcggcgctg gaatcccaca atgtggcggt
     4201 tgagtaccag tgccgcgaag gttactgcgg ctcctgtcgc acccgactgg tcgcaggtca
     4261 ggtggactgg attgccgaac cgttagcctt tattcagccg ggggaaattt tgccctgttg
     4321 ttgccgggca aaaggcgata ttgaaatcga gttgtgatcg ggtactagcg ccagatacga
     4381 cgcttacgcg tattatctgg cctacaacga ttacatggcg tagtaatacg ctttcacctg
     4441 ctcccagtcc gctttaggga tcggctccag atatttttcc agctggcgga agtcatgatt
     4501 aatcgcttta tcgcgacgca agcggcgacg acttttctcc agatcaagaa aaccagcttc
     4561 tgccttacct tctgttttca catagatatg gcgaacatag caacagccat gctgacgatt
     4621 aacgctatgc attttcttaa acgccagtgc cactgctttc aacatggctt gccgtacttc
     4681 gtcagaataa ggcgatactg catgccgggc ataccagtca gcaatgctga tgaaccccgc
     4741 catatcttca gtcaccagca acgcgcgcca ttcaccctca attttcaccg cttcaccaaa
     4801 aacgatcttc ggcacgatga caccggcccg ttccagttct ttaattaccg caacctcacg
     4861 gacaatcgtt ggtcggccga acggataacg tactgaatga aacagatgat gcgtcatgcg
     4921 ctttacatac agctttttgc cgttgcgctc gacgcattgc accccgctca taccattacg
     4981 gcgatagtta ggctcttcaa cccagtcgcc ctctgttgcc caccagtgat taaattcgtc
     5041 gtactttgct gaaactgcca taccctatcg cctgtcattt ttattaacga caatgactat
     5101 aggtggttac ctgaggaaaa tcttaatgaa acgtgtcgta ttaatgaatt tcttttgccg
     5161 ctacaggtgt gacaccgctt ttgcccttca tttgcagcgc atagattcca ccagccacta
     5221 ccactaatag tgcgcccagc agataaaatg tgaatttaat cagtcggata cagcgatgaa
     5281 ttttattcga cagggattag gcatcgcctt acaaccagag ttaacgctga aaagcattgc
     5341 aggtgaattg tgttccgttc ctcacgaacc aaccttctat cgacagattt cgttgctgac
     5401 taaagaaaag ccggtagaag gcagtccact gtttttacta caaatgtgca tggaacaatt
     5461 agtggcgatt ggaaaaattt gatatgagac aggatggcgc atgaacgcca tcctgcagta
     5521 acattactct ttattaagaa attttactgc cttatcaggg aaatcagtaa acagcccatt
     5581 tacacccgct ttgttataca gagcatcata taactgattc acatcaggag tgtattcagg
     5641 cagtttatct gaccgcacgg tataaggatg cactaccagt ttattctgct gggcatcttg
     5701 caccatgcca gtgagtttga tattacccgg ctgcgatgtc tcctcaatca acatatggta
     5761 atccggacca ataccatctg catattccgc cacctgtttc atggctcccg gcttaaacat
     5821 ccagtcgtag ttgtaattaa cccagcttcc atccggctgt ttctgctgcg tttcattcca
     5881 gtcggtatag gcaatcagct gtaccagatt gagctccatg cccattttgg gttccagctc
     5941 attcttaata cgcttcagct catcagcatc aaaacattgc aaataaactt tatcgtcttt
     6001 accggtgtaa ccatatttct tcagcacttc cagcgttttt gccgcaatat ccttcccttc
     6061 ctgatgatgg aaccacggcg ctttgatttc tggatagata ccgatatttt tcccggtaga
     6121 gtgatttaac ccctgaacaa attcaatctc ttcttcaaag gtgtgcaccc ggaagtcgga
     6181 cttacccatt gggaaacgcc ccggataagt ctgtactttt ttaccgtttt caatatcgaa
     6241 accttcggta aatttcagcg acttaatttc atccagcgtg aaatctatcg cgtagtaacg
     6301 accgtctttg cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac gatcgagata
     6361 atggtcatgc agaacaacca gatggtcgtc tttggtcatc accaaatcct gttccagata
     6421 atccgctccc tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg gcaaatatcc
     6481 actggcaccg cgatgggcga tgactatttt ttcgttgctg tccgccgcca ttgcactgct
     6541 tcccatgact atagtgctca tcatgatcgc catgctaagg tttttcagct tcaatttcat
     6601 gccattagcc tccgttgcgt ttttgcagta attgttcatg gcgacgtttt tcgccaatca
     6661 tcacaacaat caacaagata accgccagaa tgctgccgcc aatcattacc ataaagccgc
     6721 catcccagcc gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc accgaaccgc
     6781 ccaggtaacc aaacagcccg gtaaagcccg ctgccgtacc tgccgctttt ttcggtgcca
     6841 gttccagcgc atgcagaccg atcagcatca caggaccgta gatcaggaag ccgataacaa
     6901 tcatacaaat catatcgacg gttgggttac ctgccgggtt catccagtaa acgatagtcg
     6961 cgatggtcac cagtgtcata aagaagacgc cggttgcccc acggttacca cggaagactt
     7021 tgtccgacat ccagccgcac agcagggtgc ccggaatacc cgcatactca tagaagaagt
     7081 aggcccagga ggatttatcc agcgcgaaat gcttaacctc tttcagataa gtcggtgacc
     7141 agtcgaggat gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg atgtaccaca
     7201 gcagtttgtt cggcagcacg tactgcatga agatttgctt cgcggtcagc tcctgttccg
     7261 ctttttcgtt atagtcgtcc ggataatcat ttttgtactc ttcgatcggc ggcaagccac
     7321 aggattgcgg ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga atggcgcaga
     7381 aagcaggcat atagagcgcc gcatgccagt cattgaacca ggccatcccc agcaggaaca
     7441 gcagcggcgg aataccacca ccgacgttgt gcgcacagtt ccacactgac acaatgccgc
     7501 cacgttcttt ctgcgaccac cagtgcacca tagtacgacc acacggcggc caccccatcc
     7561 cctggaacca accgcagagg aacaacagta caaacatcac cgcaatgctc gacgtcgccc
     7621 atggcacaaa gcccataaac aacatcactg ccgccgccag aatcaaacct gcgggcagga
     7681 aaacgcgcgg attcgagcga tccgataccg aacccatgat gaatttcgaa aatccataag
     7741 caatcgagat ccccgaaagg gcaaaaccta aatcaccgcg tgagaatccc tgctcaacca
     7801 gataaggcat agcaagcgca aagttcttac gaaccaaata gtaagccgca tagccaaaga
     7861 atatccccag gaaaatttgc cagcgcaatc gacgataagt cggatcgatc tccgcggcag
     7921 gtaagcgcgc tttgtgtggc gctggtttaa aaatactcaa cattgatagc ctccgtggcc
     7981 cgtggtctta tttatgatta acagcctgat tcagtgagag aacctgccgt ttcttgagtt
     8041 gccgcgatgt taagaaaaca ttcataaatt aaatgtgaat tgccgcacac attattaaat
     8101 aagatttaca aaatgttcaa aatgacgcat gaaatcacgt tttactttcg aatcatgagc
     8161 gaatatgcgc gaaatcaaac aattcatgtt tttactatgg ctaaatggta aaaaacgaac
     8221 ttcagaggga taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat tatcattggc
     8281 ggcggcgcaa cgggagccgg gattgcccgc gactgtgccc tgcgcgggct gcgcgtgatt
     8341 ttggttgagc gccacgacat cgcaaccggt gccaccgggc gtaaccacgg cctgctgcac
     8401 agcggtgcgc gctatgcagt aaccgatgcg gaatcggccc gcgaatgcat tagtgaaaac
     8461 cagatcctga aacgcattgc acgtcactgc gttgaaccaa ccaacggcct gtttatcacc
     8521 ctgccggaag atgacctctc attccaggcc acttttattc gcgcctgcga agaagcaggg
     8581 atcagcgcag aagctataga cccgcagcaa gcgcgcatta tcgaacctgc cgttaacccg
     8641 gcactgattg gcgcggtgaa agttccggat ggcaccgttg atccatttcg tctgaccgca
     8701 gcaaacatgc tggatgccaa agaacacggt gccgttatcc ttaccgctca tgaagtcacg
     8761 gggctgattc gtgaaggcgc gacggtgtgc ggtgttcgtg tacgtaacca tctcaccggc
     8821 gaaactcagg cccttcatgc acctgtcgtg gttaatgccg ctgggatctg ggggcaacac
     8881 attgccgaat atgccgatct gcgcattcgc atgttcccgg cgaaaggatc gctgctgatc
     8941 atggatcacc gcattaacca gcatgtgatc aaccgctgcc gtaaaccttc cgacgccgat
     9001 attctggtgc ctggcgatac catttcgctg attggtacca cctctttacg tattgattac
     9061 aacgagattg acgataatcg agtgacggca gaagaggttg atattctgct gcgtgaaggg
     9121 gaaaaactgg cccccgtgat ggcgaaaacg cgcattttgc gggcctattc tggcgtgcgc
     9181 ccgctggttg ccagcgatga cgacccgagc ggacgtaacg tcagccgtgg catcgtgctg
     9241 ctcgaccatg ctgaacgcga tggtctggac ggatttatca ccatcaccgg tggcaaactg
     9301 atgacctatc ggctgatggc tgaatgggct accgacgcgg tatgccgcaa actgggcaac
     9361 acgcgcccct gtacgactgc cgatctggca ctgcctggtt cacaagaacc cgctgaagtt
     9421 accttgcgta aagtcatctc cctgcctgcc ccgctgcgcg gttctgcggt ttatcgtcat
     9481 ggcgatcgca cgcctgcctg gctgagcgaa ggccgtctac accgtagcct ggtatgtgag
     9541 tgcgaagcgg taactgcggg tgaagtgcag tacgcggtag aaaatttaaa cgttaatagc
     9601 ctgctggatt tacgccgtcg tacccgtgtg gggatgggca cctgccaggg cgaactctgc
     9661 gcctgccgcg ctgccggact gctgcaacgg tttaacgtca cgacgtccgc gcaatctatc
     9721 gagcaacttt ccaccttcct taacgaacgc tggaaaggcg tgcaacccat cgcctgggga
     9781 gacgcactgc gcgaaagcga atttacccgc tgggtttatc agggactgtg tggtctggag
     9841 aaggagcaga aagatgcgct ttgatactgt cattatgggc ggcggcctcg ccggattact
     9901 ctgtggcctg caactgcaaa aacacggcct gcgctgtgcc attgtcacgc gtggtcaaag
     9961 cgcgttgcat ttctcatccg gttcgctgga tttgctgagc catctgccag atggtcaacc
    10021 cgtggcagac attcacagtg gactggaatc tttgcgtcag caggcacctg cacatcctta
    10081 ctctcttctc gggccacaac gcgtgctcga tctcgcttgc caggcgcagg cattaatcgc
    10141 tgaaagcggt gcgcaattgc agggcagcgt agaactggct caccagcgga ttacgccgct
    10201 cggcactttg cgtgcaacct ggttaagttc gccagaagtt cccgtctggc cgctacccgc
    10261 gaagaagata tgtgtagtgg ggataagcgg gctgatggat ttccaggcgc atcttgcggc
    10321 ggcttcgttg cgcgaactgg atctttcggt tgaaaccgca gaaatagagc tgccggaact
    10381 ggatgtcctg cgcaataacg ccaccgaatt tcgcgcggtg aatatcgccc gtttccttga
    10441 taatgaagaa aactggccgc tgttacttga tgcgcttatt cctgtcgcca atacctgcga
    10501 aatgatcctg atgcccgcct gcttcggcct ggccgatgac aaactgtggc gttggttgaa
    10561 tgaaaaacta ccttgttcac tgatgctttt gccaacgctg ccgccttccg tgctgggcat
    10621 tcgtctgcaa aaccagttac agcgccagtt tgtgcgccaa ggtggagtgt ggatgccggg
    10681 cgatgaagtg aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct ggacccgcaa
    10741 tcacgccgat attccgctac gtccacgttt cgcggttctc gccagcggca gtttctttag
    10801 tggcggacta gtagcggaac gtaacggcat tcgcgagccg attctcggcc ttgatgtgct
    10861 acaaaccgcc acgcggggtg aatggtataa gggcgatttt tttgcgccgc aaccgtggca
    10921 gcagttcggt gtaaccactg atgagacgct acgcccgtcg caggcagggc aaaccattga
    10981 gaacctgttt gccatcggtt cggtgctggg cggatttgat cccatcgccc agggatgcgg
    11041 cggcggtgtt tgtgccgtca gtgctttaca tgccgctcaa cagattgccc aacgcgcagg
    11101 aggccaacaa tgaacgacac cagcttcgaa aactgcatta agtgcaccgt ctgcaccacc
    11161 gcctgcccgg tgagccgggt gaatcccggt tatccagggc caaaacaagc cgggccggat
    11221 ggcgagcgtc tgcgtttgaa agatggcgca ctgtatgacg aggcgctgaa atattgcatc
    11281 aactgcaaac gttgtgaagt cgcctgcccg tccgatgtga agattggcga tattatccag
    11341 cgcgcgcggg cgaaatatga caccacgcgc ccgtcgctgc gtaattttgt gttgagtcat
    11401 accgacctga tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac cgctacctcg
    11461 ctgaaaccgg tgcggcagct gcttgatgcg gcgttaaaaa tcgatcatcg tcgcacgcta
    11521 ccgaaatact ccttcggcac gttccgtcgc tggtatcgca gcatagcggc tcagcaagca
    11581 caatataaag accaggtcgc tttctttcac ggctgcttcg ttaactacaa ccatccgcag
    11641 ttaggtaaag atttaattaa agtgctcaac gcaatgggta ccggtgtaca actgctcagc
    11701 aaagaaaaat gctgcggcgt accgctaatc gccaacggct ttaccgataa agcacgcaaa
    11761 caggcaatta cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg cattccggtg
    11821 attgccacct cctcaacctg tacatttgcc ctgcgcgacg aatacccgga agtgctgaat
    11881 gtcgacaaca aaggcttgcg cgatcatatc gaactggcaa cccgctggct gtggcgcaag
    11941 ctggacgaag gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt ttatcacact
    12001 ccgtgccata tggaaaaaat gggctggacg ctctacaccc tggagctgtt gcgtaacatc
    12061 ccggggcttg agttaacggt gctggattcc cagtgctgcg gtattgcggg tacttacggt
    12121 ttcaaaaaag agaactaccc cacctcacaa gccatcggcg caccactgtt ccgccagata
    12181 gaagaaagcg gcgcagatct ggtggtcacc gactgcgaaa cctgtaaatg gcagattgag
    12241 atgtccacaa gtcttcgctg cgaacatccg attacgctac tggcccaggc gctggcttaa
    12301 actcccttct gatgcccggt aagcatgtgg ttaccgggca tttttgcgta cacgattccg
    12361 tgcccaatgt atgcgttgca acgcagtgaa aattcctctg aaaacgtctc gcaaaggctg
    12421 aaactggcag atgtcaaagg cctgggataa ccgtaatgtc gcgtcatcat aaatatcagg
    12481 tgacggacaa ccatgaccga atcaacaacc tcctccccgc atgatgcggt atttaaaacc
    12541 tttatattca cacccgaaac cgcacgggat tttctcgaaa tacatttacc agaaccactg
    12601 cgcaagcttt gcaacctgca aaccttacgc ctggaaccca ctagttttat tgaaaaaagt
    12661 ttacgcgctt actactcgga tgttttgtgg tccgtggaaa ccagcgacgg tgacggctat
    12721 atctactgcg tgattgaaca tcaaagctct gcagaaaaga atatggcttt tcggccaatg
    12781 cgctatgcca ctgccgccat gcagagtcac ctagataaag gctatgacag agttccgctg
    12841 gtggtgccat tgctgtttta tcatggcgaa acatcgccct acccgtactc acttaactgg
    12901 ctggatgagt ttgacgatcc gcaacttgcc cggcagttgt acaccgaagc ttttccgttg
    12961 gtggatatca ccatcgtacc tgacgatgag atcatgcaac atcggcgtat agctctgctg
    13021 gaactgattc aaaagcatat tcgcgaccac gatttaatcg gcatggtcga caggatcacc
    13081 acgcttttgg ttagaggctt cactaatgac agccagctac aaacactgtt taattatctg
    13141 ctgcaatgcg gcgatacctc ccgtttcacc cgttttattc aggagattgc cgaacgttca
    13201 ccactacaaa aggagagatt aatgactatt gctgaacggc tacggcagga aggacatcaa
    13261 attggctggc aagaaggtaa attagtaggt ttgcagcaag gtaaattaga aggtttgcag
    13321 gaaggtatgc atgaacaagc cattaaaatt gctttgcgca tgctggaaca gggcattgat
    13381 cgtgaccaag tgctcgcggc cacccagcta agcgaagccg atctggcagc gaataaccac
    13441 taattaacac aggcccacag ccgatcccca tgggcctttg atatcaataa ctacctttta
    13501 tgcgtggccc atttttgcct gatttaaaca tcgccagtcg ttgatccagg gcatcgctgt
    13561 agagcatcgt gtcaacgcca acagcgacaa agttcgctcc ccacgccagg cattgctgcg
    13621 ccatatcagg agccacagcc agaaaaccag ccgctttacc cgcagcacgg atccgccgaa
    13681 tgctggtttc aataattcgc tgcacttccg ggtgcccggc gttatccggg tagcccaacg
    13741 acgcagaaag atccgctggt ccaataaaca cgccatcaat cccttcgacg tcgaggatct
    13801 cgtccaggtt atccagtgcc gttttacttt ccacctgcac taacagacaa agcgaatcgt
    13861 tgacttgcgc catgtaattc tcaatgcgtc cccagcgcgc agcccgtgcc acactggccc
    13921 caacaccacg ctcaccgtag ggagggttgg acaaattttg ccggaggggg aatgtgccgg
    13981 tgcagtaaac cggcacagag tggcgaaatc agacggataa agtcatggac tcaacaacct
    14041 caatccagcc atgctcacta gcgatgtcct ggccgtttaa ccaacggcgt agcatattca
    14101 gtgccatcat tgcgcacact tcctgacgga tagccaggct gtagcgtgta gtgctgaaac
    14161 gcacacgcag agcgaaagtg ccgtctggcg tggctagcgc aaagttgaga tgctcgttct
    14221 cgaaacccga aacagccagt gccagcccgg caaaatggtt ggcccgccgt tctgtaatcc
    14281 agtgcgcagt ttgcgccagg gtttcttcct gtgaaggaat cacttcacac gccagcaatg
    14341 gagcacctgc gcgagaaagt tgcaaagcca ataaaccacc ggtgaattgc tcgctcaacg
    14401 tcaggctgaa ctggcggttt tgcaattcgc gactgatctg cgcgggcagt ccttcggtgc
    14461 cttcgaaaat cacgctctgt ccggcaacac gtttaacgtc cagccacagt ttttccatcg
    14521 cctgttgctc gcttgccggt ccggtgagtt tcagttcgat gatcggcatt gaggagcgat
    14581 agcccattgt tacgcccggc ggcagttgta gagtgtccag gctttgtgcc agatcgcttt
    14641 ccgaacgacc aaaggtagtt aaacgcagac aaaccggcgg ctgtggtaaa gaaaaacgct
    14701 cgcgcaggcg cggcaggatt tcgtgctcga ccatcacctt aaattctgac ggtacgccgg
    14761 gagtaaagaa catcaggcaa cgattaagct gcacggcaaa accacaggcg gtgcctaccg
    14821 ggttattgat aaattcagca ctggcaggca gctccgcttg tttacggttg ctcggtgcca
    14881 ttactcgtcc acgttcgtgg aaatagcgtt ccatctcttt gagccaggct tcatgcagca
    14941 ccaggccttc accttttgct gtcgcagcgg cgagtgcgct taaatcatcg ctggtcggtc
    15001 ccagcccgcc gttaacgatc agcacatcgg cgtgctgact acgttcgcga agaatggtga
    15061 ctaagtcatc aaggttatcc cccaccgtat tgcggcgaga taatggcagc ccctgatgaa
    15121 agaaaaaatc ggccagccag gcagcgttag tgtcaacgat ttgcccgtat aacacttcat
    15181 ccccggtgga tagcatttcc acttttaaca tggttatctc ctgcttttgt tatcgaatca
    15241 ctataacgca aagggagata aggcgtagta agaaactgaa tgccgcgccg gaagggatca
    15301 gaaactggcg ttaaccccaa catacaggcc gtcagccaca gcgttgtcgc ggttaccgtc
    15361 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggac gcataatggt
    15421 gtaacgcgcg ccagcattcg cttcctcata actttgaata ccgctggaga gcgaatccgg
    15481 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    15541 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaca cgccttttcc
    15601 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgcccgcgac
    15661 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaaa gccaggcccg tcgattcagt
    15721 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tggccgctga tgctcattgc
    15781 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    15841 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    15901 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    15961 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    16021 aagtgcagtt atcattgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    16081 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    16141 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    16201 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    16261 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    16321 cgccgtggac cttcagcgat gatattcgca ccaagacata tgcagatggt cgcaaggaag
    16381 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    16441 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    16501 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    16561 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    16621 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatatc gcatcacgct
    16681 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    16741 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    16801 gtttatctac cgtcagcttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    16861 cggtattact ggaataaaga tcgaaatcag agatgtcagc gctaaaagcg ttgcccagtt
    16921 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    16981 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    17041 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    17101 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    17161 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    17221 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    17281 cctacgatgt cggcaaaatg cctcaaattt tagccaaatg caaagcctaa ataagaaaaa
    17341 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    17401 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    17461 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    17521 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    17581 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    17641 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    17701 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    17761 tttccttgct gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    17821 cgcctgaagc gatcgaatca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    17881 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    17941 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    18001 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gctgaaggtg
    18061 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    18121 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    18181 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    18241 tagtcaaatt agagcacctc aacacccgtc ggcgcgaaat tgcccagcaa tatcagcaag
    18301 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    18361 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    18421 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    18481 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
    18541 aacgcatctg ttcgttgccg ctgttcccgg atatgactac cgccgatgcc gaccgcgtca
    18601 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    18661 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    18721 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    18781 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    18841 ttgtgtctat tttgattaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    18901 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    18961 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    19021 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac cggcttattc
    19081 agcgcaccac tggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    19141 atattgtcga tgcgatgttg cactgccatg aacgcagcac ctttatcccg attctggcga
    19201 atatcttcgc ccgccgtgca attgaaattc cagtacatca tgccgagcgt gagtttggtg
    19261 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    19321 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    19381 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    19441 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    19501 gattactcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    19561 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    19621 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    19681 tagcgccatt tttacccata ccgataatcc cggtgaaaaa gccttttatg gttcggtggc
    19741 tcatctggcg gcggaaagag acattccggt ttatgcgccg gataacgtta atcatccgct
    19801 gtgggtggaa cgcattgccc aactgtcacc agaggtgatt ttctcttttt attatcgcca
    19861 tcttatttgc gacgagattt ttcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    19921 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    19981 ggaaacgggc gttacattgc accgaatggt aaacgtgccg atgctggggc cattgtggcg
    20041 caactgcgcg ttgccattgc gccagacgat atcgctatta cgctgcatca taaattgtgc
    20101 catgccgcgc gccagctact ggaacagaca ttacccgcca ttaaacacgg taatattctg
    20161 gaaatcgccc agcgcgaaaa cgaagccacc tgttttggtc gcagaacgcc ggatgacagt
    20221 ttccttgaat ggcataaacc ggcatccgta ctgcacaaca tggtacgtgc cgttgccgat
    20281 ccgtggccgg gtgccttcag ctatgttggc aatcagaaat tcaccgtctg gtcgtcgcgt
    20341 gttcatcctc atgccagcaa agcacagccg gggagcgtga tttctgttgc gccactgctg
    20401 attgcctgtg gcgatggcgc gctggaaatc gtcaccggac aggcgggcga cggcattact
    20461 atgcagggct cgcaattagc gcagatgctg ggcctggtgc aaggttcacg cttgaatagc
    20521 cagcctgcct gcaccgcccg acgccgtacc cgggtactca tcctcggggt gaatggcttt
    20581 attggcaacc atctgacaga acgcctgctg cgcgaagatc attatgaagt ttacggtctg
    20641 gatattggca gcgatgcgat aagccgtttt ctgaatcatc cacattttca ctttgtcgaa
    20701 ggcgatatca gtattcattc cgaatggatt gagtatcacg tcaaaaaatg tgatgtcgtc
    20761 ttgccgctgg tggcgatagc cacgccgatt gaatataccc gcaacccgct gcgcgtattt
    20821 gaactcgatt ttgaagagaa tctgcgcatt atccgctact gcgttaagta ccgtaagcga
    20881 atcatcttcc cgtcaacttc agaagtttat gggatgtgta gcgataaata cttcgatgag
    20941 gaccattcta atttaatcgt cggcccggtg aataaaccac gctggattta ttcggtgtca
    21001 aaacaattac ttgatcgggt gatctgggcc tatggcgaaa aagagggttt acagttcacc
    21061 ctcttcctcc cgtttaactg gatgggacca cgactggata accttaatgc agcgcgaatt
    21121 ggcagctccc gcgctattac gcaactcatt ctcaatctgg tagaaggttc accaattaag
    21181 ctaattgatg gcggaaaaca aaaacgctgc tttactgata tccgggacgg tatcgaggcg
    21241 ttgtaccaca ttatcgaaaa tgcgggaaat cgctgcgacg gcgaaattat caacattggc
    21301 aatcctgaga acgaagcgag cattgaggaa ctgggcgaga tgctgctggc gagcttcgaa
    21361 aaacatccgc tgcgccatca tttcccaccg tttgcgggct ttcgcgttgt cgaaagtagc
    21421 tgctactacg gcaaaggata tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc
    21481 caccgctgcc tggactggga gccgaaaatt gatatgcagg aaaccatcga cgaaacgctg
    21541 gatttcttcc tgcgcaccgt tgatcttacg gataaaccat catgaccaaa gtaggcttac
    21601 gcattgatgt cgatgccttt cgtggcaccc gtgaaggcgt gccgcgtctg ctggaaatct
    21661 tgagtaagca taatattcag gccagcattt ttttcagcgt cggcccggac aatatgggcc
    21721 gccatctctg gcgactggtg aagccacagt ttttgtggaa gatgctgcgc tcaaacgcgg
    21781 catcgcttta tggctgggat attttactgg caggtacggc ctggccaggt aaagagattg
    21841 gtcatgccaa tgccgatatc attcgtgaag cggctaaaca tcacgaagtc ggtctgcacg
    21901 cctgggatca ccatgcctgg caagcccgta gcggtaactg ggatcggcaa acaatgatcg
    21961 acgatattgc acgcggtctt cgcactctgg aagagattat cggtcaaccg gtaacctgtt
    22021 ctgccgctgc gggctggcgt gccgaccaga aggtgatcga agcaaaagaa gcgttccatt
    22081 tgcgctacaa cagcgattgt cgtggggcca taccgttccg tccactgctt gaatcaggaa
    22141 accctggcac tgcgcaaatt ccggtgacct tacccacctg ggatgaagtg attggtcggg
    22201 atgtgaaagc agaagatttt aacggttggt tactcaaccg catcctgcga gataaaggca
    22261 cgccggttta taccattcat gcagaagttg aatgctgcgc ttatcagcat aattttgtgg
    22321 atctcctcaa acgcgcagct caggaaggcg tgacattttg ccctttaagc gaactgttat
    22381 cagagacgtt gccgctcgga caagttgttc gcggaaatat tgccggacgt gaaggctggc
    22441 tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg gtacgttacc ttatcggcat
    22501 cttcgcgttt attgcctgct attacctgtt accgatcagc acgcgtctgc tctggcaacc
    22561 cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg gcatccggcg actggattgt
    22621 gccccatctg ttagggctac gttatttcga aaaacccatt gccggatact ggattaacag
    22681 cattgggcaa tggctatttg gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc
    22741 gaccctgtta actgccgcgc tggtgacctg gtttactctg cgcttatggc gcgataaacg
    22801 tctggctcta ctcgccacag taatttatct ctcattgttt attgtctatg ccatcggcac
    22861 ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg gcgggaatgt gcagcttctg
    22921 gctggcaatg caggcacaga cgtggaaagg caaaagcgca ggatttttac tgctgggaat
    22981 cacctgcggc atgggggtga tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag
    23041 cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat ctctttattt acggctggct
    23101 ggcggttatc agttgcgtac tgacggttct cccctgggga ctggcgatag cgcagcggga
    23161 gcctgacttc tggcactatt ttttctgggt tgagcatatt caacgctttg cactagatga
    23221 tgcccaacat agagctccgt tctggtacta cgtgccggtc atcattgccg gtagcctgcc
    23281 gtggctggga ttactccccg gtgcactgta cacaggctgg aaaaaccgca agcattccgc
    23341 aaccgtctat ttgttgagct ggacgataat gccgctgctg tttttctccg tcgctaaagg
    23401 caaattgccc acctatattc tttcttgctt tgcacctctg gcaatgctgt tggcgcatta
    23461 cgcgttgctg gcagcaaaaa ataatcctct ggcgctgcgg attaatggct ggattaacat
    23521 cgcttttggc gtcactggca ttattgccac atttgtgatc tccccctggg gaccaatgaa
    23581 cacgccggtg tggcaaacct tcgagagcta taaagtcttt tgtgcctggt cgattttttc
    23641 gctatgggca tttttcggct ggtacacctt aacaaacgtc gaaaagacct ggtctttcgc
    23701 cgcgctttgc ccgctggggc tggcgttgct ggtaggattt tcaattcctg acagagtcat
    23761 ggaaggaaaa catccgcaat tttttgtcga gatgacacaa gaatcacttc agccaagccg
    23821 ctatattctt actgatagcg tcggtgttgc cgcaggtctg gcatggagcc tgcaacgcga
    23881 tgacatcatc atgtatcgcc agacaggtga gttgaaatac ggccttaatt atccggatgc
    23941 gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga
    24001 ggggattatt acactcgtgc tttcggttga ccgcgatgaa gatatcaaca gtctcgccat
    24061 tccgcccgca gatgccatcg ttcgtcagga gcgtctggtg ctgattcagt atcgtcccaa
    24121 atgatctggc taacattagt ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag
    24181 aaacaggcaa cctgctttgt ggcgataaac aaacggcgca aacatatcgt gctgtggctg
    24241 ggactggcgc tggcttgtct tggtcttgcc atgatgctct ggctgctggt cttgcagaac
    24301 gtaccggtag gcattgccta cccgatgtta agtctgaatt ttgtctgggt gacgctggct
    24361 gcagtaaaac tgtggcacga accggtatcg ccgcgtcact ggtgtggggt ggcgttcatt
    24421 attggtggca ttgtgatcct cgggagtacg gtgtaatgtg cctgatatgg ggattattca
    24481 gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt tgcggcaagt catctgccgc
    24541 cgatgacgca cttatgggat tttatcgcgg ctctgttagc ttttggcctg gatgcccgga
    24601 tactgttact cgggctgctg ggttatctgc tttccgtttt ttgttggtat aaaacgctac
    24661 ataaacttgc gctaagcaaa gcctatgcct tgctcagtat gagttatgtg ctggtatgga
    24721 ttgcttcaat ggtacttccc ggctgggaag ggactttttc gctgaaagca ctactgggag
    24781 tagcctgtat tatgagcggg ttgatgctga ttttcctgcc cacgacaaaa caacgttact
    24841 gagttttccc tgccgctgta cactgccgtt cccactcatc ggggctataa ctgcttgccg
    24901 agaggacttt caccgtatgc agcttttcac gattaataca ataaagcgca ttctgcaaag
    24961 gcgagagtaa atcccccact ttcacggcga agtcactctt cacttcggcg atcattttta
    25021 tcgcgccgcc agcatcgcag agcataagta catgtctgtt atcttgttta ttgcagcacg
    25081 attttttgtg ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    25141 tacagattaa cagataaact ctttatcaac ggcaatggaa tggcagaaat gagaagtgaa
    25201 acggagggtt aattattgct gacgttgcac ccactctttt agcgcctgac gtgaaatttt
    25261 aataccgccg tttttcagct ccggcggtaa agtcagccag cgcaccggtt gttgaaaacg
    25321 agccagctta tctttcaccc attcgccaag atcaacggtt tgctggtcat actccaccac
    25381 cgctaccggc cgatgaccaa actccttgtc ggcaacggaa acgataaaca cctgcaaaac
    25441 cgcaggatgt gcagcaataa cacgctcgac ttcttccggc tgaataccct caccgccact
    25501 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    25561 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    25621 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    25681 ccgacccggc agcggcaaac caacgtctgc caggccgtcg gcttctttcg cacataccgt
    25741 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    25801 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    25861 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    25921 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    25981 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    26041 atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa gatgggcctg
    26101 ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca
    26161 cagacgcgtc ggctgccacg cagcggcatg tacgccttca accagctgaa tgtgcagcga
    26221 cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    26281 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    26341 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    26401 catcacgccg ctgccctcaa ccaccccctg cgccgcaaag cccgtagcta actcatcgac
    26461 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggcttc
    26521 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caactgctcc
    26581 tgtacatcag cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc
    26641 tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc
    26701 cgcgccagtt gcgttagacc taagctcgac tcaatggaag aactgatcac cgccgtcagc
    26761 cccagcgcat gcgccgcctg tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg
    26821 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    26881 aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    26941 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    27001 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    27061 agatccggaa ttgcctccag caacagattc accaccatgc cgtcgcgcac cgcttcgtac
    27121 aatcccactt tgaccttcgc cactttctcg cccggcatat cggcaagttt gaggatcaga
    27181 tcgtccggat cgccattaca cagcggtgcc gcacggtagt tggctgcttg cggcaacgta
    27241 tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca cggaaggcat ctgcggtagc
    27301 tcgcaatcgc ctgccagcca gttatttacc caggcaagca gcacgctttg cgcctcttcc
    27361 cagttttcct ggctgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgccc
    27421 tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccacc
    27481 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    27541 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    27601 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcagc cccgcctgcc
    27661 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    27721 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    27781 tgacaaggcc catatccagc gcctgttttg cgtcgtactg acggcacagg aaccagattt
    27841 ctcgcgcttt tttctgcccg acgatacgag ccatgtagga agcgccccag ccgccgtcga
    27901 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgctgcg atagtcaggt
    27961 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    28021 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    28081 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    28141 acgctttatc gcctgcgcca gtcagaatga tcacgccgat attgtcgtca taacgcgcat
    28201 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    28261 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcga
    28321 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaagg cattgcttca tcaggataaa
    28381 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga ttacgccagc
    28441 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    28501 ttccgccgcc agggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    28561 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    28621 catggcggca agggttgcgc cattattgtt gctgcgcagc gccaccagct cccggcgttg
    28681 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    28741 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcacgttg
    28801 ttcagcattt tgcagccccg gatgcccgcc ttcgacaata accccacaaa gccccgccag
    28861 cccctggcaa gccgccatca tcgccacccg tccgccaagc gagtacccca ccagccagaa
    28921 gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag tcggtgacat catcaaatcc
    28981 atcgacgcta atcgccgccg aaccaccatg acctaggaga tcaacataca accgtgagta
    29041 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    29101 aaacaccagc cagggtaaac ctagttttcc gtgttttgcc tgcgcgtgca ggatcataaa
    29161 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    29221 atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg caaatgccgt ttcaagttcc
    29281 tgccagtttt gcggatgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    29341 acgttttgcg gcatcagata gaaacgctca cgctcgcttt gcggcgttgg caacagcgag
    29401 aaaatttgcc cgccgttgtt gttcaccaca attaatacca gcggcgcaga aacctgacgc
    29461 aataacgcca gcgcgttgag atcgtaaagc gcggagagat cgcccacaat cgccagcgtc
    29521 ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa gcagtccgtc gataccgctg
    29581 gcccctcggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    29641 cgtaccacca ggctgttacc aacaaacaat tgcccctgtt caggcagata gtcgctgatg
    29701 cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg caataaccgc ctgcattgcc
    29761 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    29821 tccagccagt cggcaatatt ggcaattaag cgacgtccgc gatggtgtgc cggatcaagt
    29881 cgcccttcaa tgtcatcaac aatccagtac tcttctggtt cacagcttgc ctgccattgc
    29941 aggagccgtt tccccgtcag gctacttccc agttgcacca caatttgcgc ctgctgcagc
    30001 tcgctggtcg ctttggcatt gcctaaccaa agatcggcac acggcagcgg ctgcccggtt
    30061 tgtgacagca catcgccaat cagcggccag ccaagagttt gcgcccacag ggcaactttt
    30121 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgcttttg tcgccagaag
    30181 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    30241 tcctgccacc agtcacccag acgctgttgc cagctaagcc cggtatcgtc catttcgcca
    30301 tacagcggtt cagcaaacgg gcagttgata tggactcccc ccgcatgaag cgtaccgaga
    30361 gcgtggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcaat
    30421 gaaatactgt gcgtggggtg agaggcgaac attcccggct ggcgaattgc ctgattcgcg
    30481 ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga gaatcagttt ttctccggtt
    30541 aacccggctt caatcagtgc cggatagaga tttgccaccg ccgtgccgga ggtcacaatc
    30601 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    30661 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    30721 gtagaacgcg agcctggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    30781 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    30841 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    30901 tgcgactcat tactattcca tttgtaataa agtacgcagc cctgccgctt tgttgtcgat
    30961 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catataatcg
    31021 cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc tttgttgtaa
    31081 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    31141 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggca gcaactgatg
    31201 taaacagatc acatcatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    31261 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    31321 taatcgttgg cagatatctt ccaccaccag catgttctcg cgctggtttt tatcatccgc
    31381 catcagccac tctcctaact gctgcgcctg cttatcatca ggatgatttg ctactgttcc
    31441 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    31501 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    31561 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    31621 tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    31681 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    31741 taacccaggc aagggcttga tactcaccag tgtggcgata aattcttttg cctgaatcgc
    31801 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    31861 gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc gacgggtcaa aagcattcag
    31921 cccccaaatg cgtaagtcag cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    31981 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    32041 ttgccagtag aattgcgggt acgtttgctg actggccagc cagctcaagg catcaaaagc
    32101 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    32161 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    32221 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    32281 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccgacggccg cgcccacacc
    32341 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaacag cctgcttcgc
    32401 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    32461 ttctgcatgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    32521 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    32581 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagagt
    32641 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    32701 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    32761 cgccccgagg taaacaggct tatcaggcca gtgatgcgta catgtttcga gtgttttact
    32821 catcagctgc tgccccactt tttcaccacg caacgcttca ctgacaatca cccgacctat
    32881 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatacgcca ccagttcatc
    32941 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    33001 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    33061 ctgggaaacc gataattcag agtgatgcag atcttgccat tcaatcataa tgctctcctt
    33121 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    33181 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    33241 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    33301 ccagcatcag ctactgcata ccgcctttaa ccctggaaaa ctggacaaga tttttatcag
    33361 tcaccttcat ggcgatcatc tttttggttt acccggcttg ctgtgcagtc gttctatgtc
    33421 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    33481 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    33541 tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct tatccgctgg aacacccact
    33601 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    33661 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    33721 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    33781 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    33841 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    33901 caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg aggctggagt
    33961 cggcaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcaacacct
    34021 gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt
    34081 taacgtttaa ctctgcttat tattaaaaac agggtgaaac ttgccctgtt atagcaaccc
    34141 gcgctacata tatccaccca cacaggcata ttgagcgatt tttgatattt attcatctcc
    34201 ttacgcacta tattcagagc aataagttgc ctcttttcat cttgattcag cacaaccttt
    34261 gcaactaaaa ggagcccatg atggttacag ttgtcagcaa ttattgtcaa ttatctcaaa
    34321 cccaactcag tcagacattt gcagaaaaat ttactgtgac cgaggaatta ctgcagtctt
    34381 taaaaaaaac agcgttatcc ggagatgaag aaagtattga gttactgcat aatattgcgt
    34441 taggttatga tgaatttgga aaaaaagctg ggtaatgctg ccaacttact gatttagtg
//
LOCUS       DAZOZX010000030        34095 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZOZX010000030 DAZOZX010000000
VERSION     DAZOZX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.030"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..2098
                     /locus_tag="ACHUPT_001432"
     CDS             <1..2098
                     /locus_tag="ACHUPT_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0819061.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHUPT_001433"
     CDS             2095..3183
                     /locus_tag="ACHUPT_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0819062.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHUPT_001434"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHUPT_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR0819063.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHUPT_001435"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHUPT_001435"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR0819064.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHUPT_001436"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHUPT_001436"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0819065.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHUPT_001437"
     CDS             8051..8602
                     /locus_tag="ACHUPT_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0819066.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHUPT_001438"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHUPT_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0819067.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHUPT_001439"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHUPT_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0819068.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACHUPT_001440"
     CDS             complement(11683..12681)
                     /locus_tag="ACHUPT_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0819069.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACHUPT_001441"
     CDS             complement(12760..13452)
                     /locus_tag="ACHUPT_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0819070.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACHUPT_001442"
     CDS             complement(13529..14032)
                     /locus_tag="ACHUPT_001442"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0819071.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHUPT_001443"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHUPT_001443"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0819072.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACHUPT_001444"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACHUPT_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0819073.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACHUPT_001445"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACHUPT_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0819074.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHUPT_001446"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHUPT_001446"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0819075.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHUPT_001447"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHUPT_001447"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0819076.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATQEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHUPT_001448"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHUPT_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACHUPT_001449"
     CDS             complement(22127..23560)
                     /locus_tag="ACHUPT_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0819077.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACHUPT_001450"
     CDS             complement(23623..24072)
                     /locus_tag="ACHUPT_001450"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0819078.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHUPT_001451"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHUPT_001451"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR0819079.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHUPT_001452"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHUPT_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0819080.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACHUPT_001453"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACHUPT_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHUPT_001454"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHUPT_001454"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0819081.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACHUPT_001455"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACHUPT_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHUPT_001456"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHUPT_001456"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0819082.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHUPT_001457"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHUPT_001457"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
BASE COUNT         8315 a         8787 c         8944 g         8049 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacta tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttcctgcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgct
//
LOCUS       DAZOZX010000031        33698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZOZX010000031 DAZOZX010000000
VERSION     DAZOZX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.031"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHUPT_001458"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHUPT_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUPT_001459"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUPT_001459"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0819083.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHUPT_001460"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHUPT_001460"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR0819084.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHUPT_001461"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHUPT_001461"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR0819085.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHUPT_001462"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHUPT_001462"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR0819086.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHUPT_001463"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHUPT_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR0819087.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHUPT_001464"
     CDS             complement(8300..11167)
                     /locus_tag="ACHUPT_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0819088.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(12011..13102)
                     /gene="ychF"
                     /locus_tag="ACHUPT_001465"
     CDS             complement(12011..13102)
                     /gene="ychF"
                     /locus_tag="ACHUPT_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0819089.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(13219..13803)
                     /gene="pth"
                     /locus_tag="ACHUPT_001466"
     CDS             complement(13219..13803)
                     /gene="pth"
                     /locus_tag="ACHUPT_001466"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0819090.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            14081..14359
                     /gene="ychH"
                     /locus_tag="ACHUPT_001467"
     CDS             14081..14359
                     /gene="ychH"
                     /locus_tag="ACHUPT_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0819091.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14414..16093)
                     /gene="dauA"
                     /locus_tag="ACHUPT_001468"
     CDS             complement(14414..16093)
                     /gene="dauA"
                     /locus_tag="ACHUPT_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0819092.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(16218..17165)
                     /gene="prs"
                     /locus_tag="ACHUPT_001469"
     CDS             complement(16218..17165)
                     /gene="prs"
                     /locus_tag="ACHUPT_001469"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0819093.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(17316..18167)
                     /gene="ispE"
                     /locus_tag="ACHUPT_001470"
     CDS             complement(17316..18167)
                     /gene="ispE"
                     /locus_tag="ACHUPT_001470"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0819094.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(18167..18790)
                     /gene="lolB"
                     /locus_tag="ACHUPT_001471"
     CDS             complement(18167..18790)
                     /gene="lolB"
                     /locus_tag="ACHUPT_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0819095.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            19004..20260
                     /gene="hemA"
                     /locus_tag="ACHUPT_001472"
     CDS             19004..20260
                     /gene="hemA"
                     /locus_tag="ACHUPT_001472"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0819096.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            20302..21384
                     /gene="prfA"
                     /locus_tag="ACHUPT_001473"
     CDS             20302..21384
                     /gene="prfA"
                     /locus_tag="ACHUPT_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0819097.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            21384..22217
                     /gene="prmC"
                     /locus_tag="ACHUPT_001474"
     CDS             21384..22217
                     /gene="prmC"
                     /locus_tag="ACHUPT_001474"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0819098.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            22214..22606
                     /gene="sirB2"
                     /locus_tag="ACHUPT_001475"
     CDS             22214..22606
                     /gene="sirB2"
                     /locus_tag="ACHUPT_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0819099.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            22610..23419
                     /gene="sirB1"
                     /locus_tag="ACHUPT_001476"
     CDS             22610..23419
                     /gene="sirB1"
                     /locus_tag="ACHUPT_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR0819100.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            23455..24309
                     /gene="kdsA"
                     /locus_tag="ACHUPT_001477"
     CDS             23455..24309
                     /gene="kdsA"
                     /locus_tag="ACHUPT_001477"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0819101.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24458..24565)
                     /locus_tag="ACHUPT_001478"
     CDS             complement(24458..24565)
                     /locus_tag="ACHUPT_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0819102.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(24970..26070)
                     /gene="chaA"
                     /locus_tag="ACHUPT_001479"
     CDS             complement(24970..26070)
                     /gene="chaA"
                     /locus_tag="ACHUPT_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0819103.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            26340..26570
                     /gene="chaB"
                     /locus_tag="ACHUPT_001480"
     CDS             26340..26570
                     /gene="chaB"
                     /locus_tag="ACHUPT_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0819104.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26728..27423
                     /gene="chaC"
                     /locus_tag="ACHUPT_001481"
     CDS             26728..27423
                     /gene="chaC"
                     /locus_tag="ACHUPT_001481"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0819105.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(27467..27820)
                     /gene="ychN"
                     /locus_tag="ACHUPT_001482"
     CDS             complement(27467..27820)
                     /gene="ychN"
                     /locus_tag="ACHUPT_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0819106.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            28005..29399
                     /gene="ychO"
                     /locus_tag="ACHUPT_001483"
                     /gene_synonym="iatA"
     CDS             28005..29399
                     /gene="ychO"
                     /locus_tag="ACHUPT_001483"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0819107.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(29400..30050)
                     /gene="narL"
                     /locus_tag="ACHUPT_001484"
     CDS             complement(29400..30050)
                     /gene="narL"
                     /locus_tag="ACHUPT_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0819108.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(30043..31839)
                     /gene="narX"
                     /locus_tag="ACHUPT_001485"
     CDS             complement(30043..31839)
                     /gene="narX"
                     /locus_tag="ACHUPT_001485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0819109.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            32178..33569
                     /gene="narK"
                     /locus_tag="ACHUPT_001486"
     CDS             32178..33569
                     /gene="narK"
                     /locus_tag="ACHUPT_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0819110.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         8273 a         8329 c         8585 g         8511 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
    11401 tattccaatt cgttactatg aatatggcaa aaatattaca acaaacagcg gttaaaaaat
    11461 ttggtgaaga ttttttgtaa taatcctaat tagttatggg attttcgctt gatgtatctg
    11521 cataacaaaa acaatatgcg ccatttttgc attactaata agaaaaagca aaaaacatcg
    11581 atattcattt ttgttcaatg gattttctgg cctatcgtta ttattgtgct actggttatc
    11641 attttcatct tgccaggatc gcacgtaatc acgttattcg gggactttat ctgcgggata
    11701 ttacccttca ggtaagtcct tttctcgcca gcatattgga gcgagatcaa tattgatcag
    11761 ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg ttatatcact gctaatgaga
    11821 tatcagtcat ctgaacactt atgtctttaa ttattctcgt ggttcactgt aggcaataag
    11881 cacaaaagtg taggatgtta caagaatgat taggactcgg tgaaatgaaa aatccacgca
    11941 attgcgtgga ttttatatac ttttgcgctc ttcatgagat ttagcgaaac ctcatgagac
    12001 aataaattaa ttagacgttg aaaaggaagt tcatcacatc gccatctttc acgatgtaat
    12061 ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct ttgtaagtga
    12121 tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa tcagtatgga
    12181 ttttgcccgc tgcctgcggc gcggttgctc caaccggaat ggtccatgca cgcacttctt
    12241 tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt ataaccggca cggatcacac
    12301 ggttcaggcc cggttcttcc agcccaagct cctgcataaa ctcgtcacgt tcttcgtcgt
    12361 ccagttcggc aatgtctgct tcaacagcag cacaaaccgg aaccacaaca gaaccttctt
    12421 tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt ttcaaaacca tcttcgttga
    12481 cgttggctat gtacattgtt ggtttcagcg tcaggaagct caggtaacga atagccgctt
    12541 tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc gttttccagc tggggcaggc
    12601 atttttccag gaccgccagc tcagctttcg cgtctttatc gccacctttg gctttcttct
    12661 gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt tcggtgttga
    12721 taacttcaat atcgtcagcc gggttaactt tgccggaaac gtgaatgatg ttgtcatttt
    12781 caaagcagcg aacaacgtga ccgatcgctt cggtttcacg gatgttggtc aggaactggt
    12841 tacccagacc ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg acaaattcca
    12901 tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc cagttgatcc aggcgagggt
    12961 caggcattgg tacgacgcct gtgttcggct caatggtgca gaatggaaag ttggccgctt
    13021 caataccggc tttggtcagc gcgttgaaca gggtagattt cccgacgttg ggcaaaccga
    13081 cgataccgca tttgaatccc atgatttaaa tcaccttaat atcttaataa tcaacctgtt
    13141 attgctaaca gattgcagaa atggaaataa ctttgcctat tatacacggc actcggcaaa
    13201 aatgccgcag acaacgactt attgcgcttt aaaggcgtgc aatcggttcg ttgctttggt
    13261 caagccatct gtaaaccaca tttcagtaca acgcgccgct tcgtcgatgg cttcatcaat
    13321 taacttctgt tcactaacag gcggtttgcc taacacaaaa ccgacaactt tatttttatc
    13381 gcccggatga ccgattccga tgcgtaaacg gtgaaagtta gggttattac ccaatttact
    13441 gatgatgtct ttcagtccat tgtgaccacc atggccaccg cccaacttaa atttggcgac
    13501 gccaggaggc agatccagtt cgtcgtgggc caccagaatt tcgtccggat taatgcggaa
    13561 aaaactggcc atcgccgcaa cggctttgcc gctgagattc ataaatgtag tcgggactaa
    13621 caggcggaca tcttcgcttc caagggtgac tcgcgaagta taaccaaaga atttagcctc
    13681 ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca acgaaccagg caccagcatt
    13741 atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg ccgacaatca atttaatcgt
    13801 cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta gtttactggt tgcggccccg
    13861 cttgacaaaa aactgcgtat caaatgcaga taacgtaata attgcctgag tggactatta
    13921 gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt tgaaataagg gttgtaattg
    13981 tgattacgcc cgcacataac ccacagggtg ttgtctatac tttacacata aggaagaggg
    14041 gtattccctg ttacaaccca gaaagttccg gaggtgacat atgaaacgca aaaacgcttc
    14101 gatactcggt aacgtgctca tggggttggg tctggtggta atggtggtcg gcgtggggta
    14161 ttcaatcctc aaccagttac cacagtttaa tatgccccag tatttcgcac atggtgcagt
    14221 gctaagtatt ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg gcgggcatga
    14281 acaggtgtgc gaccgttact ggtgggttcg tcactatgac aaacgttgcc gccgtagcga
    14341 taatcgccgt catagctaac aaataatgca gtttgctgac cagtcagttt gcgctgactg
    14401 gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg attcgggaag aacgcgaggc
    14461 gtcccgggat cggttgaatg cctgcgcgag ccatagttcg cagtggctgg aattccacgt
    14521 tgcacacgcg cagttcacat ccttcgggca gacgcttcac aaaacgctgg aacgcatcaa
    14581 gaccaccagc atcaagtacc ggaacggcat cccacttcag aatcacaatc cgtttgcctt
    14641 caagacgtga ctccaggtcc gtgaataagc cttcagcagc agcaaaaaac agcgggccaa
    14701 taacgcgcag aaccaggaca tcgtctggaa catctacgac taccggtgcc aggcgagtca
    14761 tacgtgcgat acgacgcata aacagcagcg atgccagcac gatccccacg ctgatggcaa
    14821 taaccatatc aaacagcacg gtcagtgaca tacacagcag catgacgatg atgtcatctt
    14881 tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc actcatgttc cacgccacca
    14941 tcaacagcag ggctgccatg gcggaaagcg gcagccagga gagcagtggt gccagtacca
    15001 gcagggcgag aataaccaga atagagtgga tcaccgccga gataggggaa gttgccccgg
    15061 cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc ggtaatacca ccaaagaatg
    15121 gagcgatgat attccccagc ccctgaccga ccagttcact attcgccttg tgcttagtcc
    15181 cggtcatacc atccagtacc acagcgcaga gcagagattc gatcgccccg agcattgcca
    15241 ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca ggttagcgtg aattctgaat
    15301 taggcagatc ccacggcagc accagttgcg gcagcagttg cggaataccg ttaccctgag
    15361 aaccatcggc caggacgtag tggaattgcg aaccgatggt agcaacatgt ccgccgagca
    15421 ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac
    15481 gaatgcccag acgcggccag aaaaccagaa tgccaagcgt cacaatgcca atggcagcat
    15541 cacccacatt aatggtcggc agcgccataa ataatgcgcc gactttttgt agataatgtt
    15601 ccgggacatg ggccatttgc agaccgagaa aatctttaat ctgcatggta ccgatggtga
    15661 tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg aatatactca atcagacgac
    15721 caaagcgtgc cagccccatc agaatcaaaa agattcccga caacaaggtc gcaaccagca
    15781 gtcctgccag tccaaactgt tgcgaaacgg gatagagaat taccacaaat gccgcagtcg
    15841 gaccggaaac gctaaagcgt gacccacccg ttagagcaat gacaatcccc gcaacagctg
    15901 cggtatataa accgtactgg ggggccacac cactaccaat agccaacgcc atcgccagcg
    15961 ggatagcaat aatcccgacg gttatcccgg caatcaggtc acgggtaaac cgtgcggcag
    16021 tatatttttc tttccagcaa acgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa
    16081 atattttgtt cacaataatg tttcatccgt gagcgcatca tctgtcaact aaatggcagg
    16141 tgaaggaggc ataggtcata caaatggata ttacagacaa aaaaacccgc cgcagcgggt
    16201 ctttgagccg ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata
    16261 cgacgaatcg cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat
    16321 ttgatttcat cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta
    16381 cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt
    16441 ttagcaccac gttctttcag cgcttcagca gctttacaca gcgtaccgcc agtgtcgatc
    16501 atatcatcga ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgg
    16561 gaaacgttcg cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc
    16621 ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta
    16681 tccagattca gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc
    16741 ggaacgtcga agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca
    16801 acaccgacgc tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa
    16861 cggacgcgac ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct
    16921 gcggaagcac gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta
    16981 ggggcacaag tggactggat gatgaaaata tcaccaccgc gtacattttc gttaatttgt
    17041 acgctgactt cgccgtcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg
    17101 cggttggcaa tacgttgtgc tagtaccggg gtggcgttac cagcaaaaag cttcatatca
    17161 ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc
    17221 gggccaggca tgatcctttc caggcggtgt attaaagagc gcgatgcaac gtctggaaca
    17281 aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg
    17341 gaaagattaa cgcctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc
    17401 tggcgggctt cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg
    17461 cgtgacgggg cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt
    17521 cttgcgataa cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt
    17581 tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg aatacttaca
    17641 ccagggtgcg ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca
    17701 acgccttcgg caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc
    17761 agccccattt ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc
    17821 accaggaccg tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc
    17881 ttgtcaatgc tgatattcgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt
    17941 ttcatcaaca accgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg
    18001 ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa
    18061 tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg
    18121 tataaaaaca gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc
    18181 agttatccat ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca
    18241 tcgcaggttg cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct
    18301 ggctgtaggt aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac
    18361 ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt
    18421 tgccaatcat ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact
    18481 gcacgttacc cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca
    18541 gcagacggta gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt
    18601 ggtcagaaat ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt
    18661 gctgatgctg acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa
    18721 cggaacaggc agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg
    18781 gcaggggcat agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag
    18841 cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt
    18901 cggcaattac tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc
    18961 aagcagacta accctatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact
    19021 cggtatcaac cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga
    19081 taagctcgat caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt
    19141 gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca
    19201 agaggcgtta atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa
    19261 aagcctctac tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg
    19321 cctggattca ctggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgt
    19381 cgattcgcaa aaaggtcata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt
    19441 ctctgtagcg aaacgcgttc gcactgaaac agatatcggt gccagtgctg tgtctgtcgc
    19501 ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt
    19561 gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt
    19621 acagaagatg attatcgcca accgcactcg cgaacgtgcc caaattctgg cagatgaagt
    19681 tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat
    19741 catcagttcc accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt
    19801 aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga
    19861 gccggaagtt ggcaaactgg cgaatgctta tctttatagt gtggacgatc tgcaaagcat
    19921 catttcgcac aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc
    19981 tcaggaaacc agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg
    20041 cgagtatcgc agccaggcag agcacgttcg cgatgagtta accgccaaag cgttagcggc
    20101 ccttgagcag ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa
    20161 ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga
    20221 acgcctgaat attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt
    20281 ttacagggtg catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat
    20341 gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa
    20401 cgttttcgcg cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc
    20461 gactggcaac aggttcagga agatatcgaa accgcacaga tgatgctcga cgatcctgaa
    20521 atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa
    20581 cagcaattac aggttctatt actaccaaaa gatcctgatg acgaacgtaa cgccttcctc
    20641 gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt ttgcaggcga tctgttccgt
    20701 atgtacagcc gttacgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag
    20761 ggtgaacatg gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt
    20821 cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg ttcctgctac ggaatcgcag
    20881 ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa
    20941 ctgccggaca tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt
    21001 ggtcagcacg ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt
    21061 gttgttgaat gtcaggacga acgttcacaa cataaaaaca aagcgaaggc gctttcagtg
    21121 ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc aacaggccga agcgtctacc
    21181 cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta accgtactta caatttcccg
    21241 caggggcgcg ttaccgatca ccgcatcaac ctgacgctct accgcctgga tgaagtgatg
    21301 gaaggtaagc tggatatgct gattgaaccg attatccagg aacatcaggc cgaccaactg
    21361 gcggcgttgt ccgagcagga ataatggaat atcaacactg gttacgtgaa gcaataagcc
    21421 aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat cctgctggag catgttaccg
    21481 gcagagggcg tacttttatt ctcgcctttg gtgaaacgca gctgactgac gaacaatgtc
    21541 agcaacttga tgcgctactg acacgtcgtc gcgatggtga acccattgct catttaaccg
    21601 gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc agcgacctta attccgcgcc
    21661 cggatacgga gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta
    21721 ttctcgatct cgggacgggt accggggcga ttgcgcttgc gctggctagc gagcgcccgg
    21781 actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt ctccctggca caacgtaatg
    21841 cccagcatct ggcgatcaaa aatatccaca ttctgcaaag cgactggttt agcgcgctag
    21901 ccgggcagca gtttgcgatg attgtcagca atccgccgta tattgacgag caggacccac
    21961 atcttcaaca aggcgatgtc cgctttgagc cgctcactgc gctggttgcg gcagacagtg
    22021 gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta tccggcggct
    22081 ttctgcttct ggaacatggc tggcagcagg gcgaagcggt gcgacaagca tttatcctcg
    22141 cggggtatca tgacgtcgaa acctgccgtg actatggtga taacgagcgc gtaacgctcg
    22201 gccgctatta tcaatgacaa gtttttctac actgcttagt gttcatctta ttagtatcgc
    22261 gctttctgtt gggctattaa ccttacgttt ctggctacgt tatcagaagc atccacaggc
    22321 atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg
    22381 cattgcgttg atggctaaag cgcacatcct gccattttcc gggcaggcac agtggctgac
    22441 tgaaaagctg tttggagtta tcatttatat cgttttgggt tttattgcac tcgattatcg
    22501 tcgtatgcac agtcagcagg cgcgcattat tgccttcccg ctggcgttgg tggtgctgta
    22561 catcatcatt aaactcgcca ccacaaaagt accgttactg gggtaagtca tgagatcgtt
    22621 agctgatttc gaatttaata aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc
    22681 aatccgccgc gattttccct cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct
    22741 ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag cagctggaaa aactgatcgc
    22801 gctgttttac ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc
    22861 attatggctg gaccaggtgt taaagaatcg acagggcagt gcggtatcat taggtgcggt
    22921 tttattatgg gtcgcgaatc gtctcgattt gccgctgctg ccggtgattt tccctacgca
    22981 gctgatattg cgcattgaat gtccggatgg cgaaatttgg ctgattaatc cttttaacgg
    23041 tgaatcgtta agcgaacata tgctggacgg atggttaaag ggaaatatca gcccgtcggc
    23101 ggaactgttt tatgaagacc ttgatgaagc tgataacatt gaggtaatcc gcaaattgct
    23161 ggatacactc aaagcctcgt tgatggaaga aaatcagatg gagctggcgt tacgcaccag
    23221 cgaagcttta ttacaattca accctgaaga tccctatgaa attcgcgatc gtgggttgat
    23281 ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat ttaagttatt tcgttgaacg
    23341 gtgtccggaa gacccgatca gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa
    23401 acatattgtg ctgcattaat taatcgacat tttactcaag attaaggcga tcctatgaaa
    23461 caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg acctgccgtt cgtactgttt
    23521 ggcggtatga acgtgctgga atctcgcgat ctggcgatgc gcatttgcga gcactacgta
    23581 actgtgaccc agaaactggg tatcccttac gtgttcaaag cctcttttga caaagccaac
    23641 cgctcctcca tccactctta tcgtggaccg ggcctggaag aagggatgaa aatcttccag
    23701 gagttgaagc agacttttgg cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca
    23761 cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg cgtttcttgc tcgccagact
    23821 gacctggttg aagccatggc gaaaaccggt gcggtaatta acgtcaagaa accacagttt
    23881 gtcagcccgg gacagatggg taatatcgtt gataaattca aagaaggcgg caacgaaaaa
    23941 gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca acctggttgt cgatatgctg
    24001 ggcttcagca ttatgaagaa agtgtctggt aactcgccgg tgattttcga cgtgacccac
    24061 gcactgcaat gccgcgatcc gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct
    24121 gagctggcac gagccggtat ggcggtaggt ctggcggggc tgtttattga agcgcatccg
    24181 gatccggaac atgcgaaatg tgatggtcca tccgcgctgc cgctggctaa actggaaccg
    24241 ttcctcaagc agatgaaagc gattgatgat ctggtgaaag gtttcgaaga actggatacc
    24301 agcaagtaat cttttttgct tgaaaaataa agtcttagcg ttctgcgtta agactttttt
    24361 catgggtgcc agatacaaaa aaggccgcag gctgttaccc ctgcggccgg tttcgggcgc
    24421 atattgccat cacggcagcc tgacgcccgt tttcacctta cttccggtta cgccaccagc
    24481 tgacaatcgc tgcggcaata atccccgcca ggatcggtgc tgccaggtcg tgccagaaag
    24541 tcatggcaaa ctgcgcgagc gtcatatagc cgccttgttg taatgacaac attttgcggc
    24601 tattcttgaa gtgtctggtt tcaagattag cccccgtttt gttgtcaggt tttacctctc
    24661 aacgtgcggg ggttttctct ttccagcaac caatgccacc agggataaag cccccgcaac
    24721 attgcgcctc accggataat tccggcttgg tgtggatact acgtctcaat tcatcttcac
    24781 ttcatccctg aaatgtttgc aataaagagt acattccggc ttttcaacag ctgttgcagt
    24841 ggtttcgtgc aggttgtggt acaggctcgc aattctgatt aacgagctgt ccaggtagta
    24901 tgaagaaata caataagcag gaatttatct tatctctccc gatttccgtc atcggaggag
    24961 atataactgt caggcaaata tcgtcatcaa ataggcggca aacagtgcca gatgcgctgc
    25021 gccattgagc acgttagtac gtccggtgga gaaggagata tggcacagca ctaaagaggc
    25081 caccatcacc accatttctg gcgcaccaag tgcaaactgc aattcgttac ccgtcataaa
    25141 ggcaattagc gtgacgacag gtacggtaag cgaaattgtt gctaacactg aaccaaagaa
    25201 cagattcatc gcgcgctgaa cctggttgtt caacactgct tttaacgcac ctaaaccttc
    25261 cggcgacaga atcaacagtg ccaccaggaa gccagtaaag gcaacagggg cattcatgct
    25321 gtcgagcaat gtctccagcg agctggcgtt cattttggtg actgcaataa cggcaatcag
    25381 atggataatc aaccagatag catgccacag gctgctatgg gcagacgttt taccgtggtg
    25441 cgggtcgtcg tcatcactgt catcttcgtg ctcgtagaca aacaaacttt gatgcgtttt
    25501 ggcctggatc agcaaaaata cgccatacat tgccgcagaa attaatgcta ccagtaacgc
    25561 ctgaccggtt gaaaaattcg ccgcaggcag agccattgga aataccagta cgattatcgc
    25621 cagggggaac agcgcaatta aatactgctt gataccaaac agattcatat attgggtggc
    25681 aaacttacga ccgcccaaca ataatgaaaa gccaaccagc ccaccggtaa caatcataat
    25741 gattgaatag agcgtatcac gcattagcgt tggcgcggcg tcgccggttg ccattaaagc
    25801 tgaaatcaaa ctgacttcaa gaataaccac tgaaaggcta agaataagcg aaccgtaagg
    25861 ttctcccagg cgatgggcta atacgtccgc atgacggaca acactaaagg cgctacttaa
    25921 aataccaata agcgcaagaa gattgatggc aatgaccact ggtagtgtct ggctacttcc
    25981 ccacaggaac agcactacca gcaccagaac cgggaaaata agcgaagtct ccttgtggcg
    26041 ggtttttacc gcctcttgag catttgacat tatggttatc cctttgcaga tgaatttatc
    26101 gaaaatgtaa aaaataggta ggaaaaataa cagaaagtgt ctggatatcg gtaacatttt
    26161 acgaattttt accccgctgt cgatttttta ctcattgggg cataaaaata agtattacgt
    26221 ttagacaatg tttgttttag cgtttcttaa gaacagtctg acatgaaaat tcttatgttt
    26281 tggcaagagt agatattgtt gaccacactt aatgttcaac tttgtaaaag gagtcaacga
    26341 tgccgtataa aacgaaaagc gatctgccgg aaagcgtaaa gcacgttcta ccgtctcatg
    26401 cccaggatat ctataaagaa gcgttcaaca gcgcatggga tcaatataaa gataaagaag
    26461 atcggcgtga tgacgccagt cgcgaagaaa ccgcgcataa agtggcctgg gctgctgtga
    26521 agcatgaata tgccaaaggg gatgatgata aatggcataa aaaatcgtaa aaccggtagc
    26581 ttagttaaag ctattcgtgc ggtgttgcct tgcaagtggt ccgtggattg catattgttc
    26641 cgttagtggt ttcaaaatga gcggtaaaaa tgtccggaag acaccaaaaa gttgtcgcag
    26701 ggaagtatgc agtggcggag gtgtaaggtg ataacgcgtg atttcttgat gaatgccgat
    26761 tgtaaaacgg catttggtgc cattgaagaa tcactcttat ggtcagcaga acaacgggcg
    26821 gcttcgctgg cggcgacgct ggcttgtcga cctgatgagg gaccggtgtg gatcttcggt
    26881 tatggatcgt tgatgtggaa tccggcactg gagtttaccg aatcatgcac cggtacactg
    26941 gttggatggc atcgcgcgtt ttgcctgcgc ctgaccgccg ggcgcgggac tgcgcaccag
    27001 ccgggacgga tgcttgcact gaaagagggc ggacgcacca caggcgtcgc ctatcgacta
    27061 ccagaagaga cgctggagca ggaactaacc ctgttgtgga agcgcgagat gattaccggc
    27121 tgttatctgc caacctggtg tcagcttgat cttgatgatg gacgcacagt aaacgccatt
    27181 gtgtttatta tggacccgcg acatccagaa tatgaatctg acactcgcgc tcaggtcatc
    27241 gcgccgttga ttgcggcggc gagcggtccg ctgggaacca atgcacaata cctgttttca
    27301 ctggaacagg agcttatcaa actgggaatg caggacgatg ggctgaatga tttgctggta
    27361 tcggtaaaaa aactactggc ggagaattat ccggatggtg tgttaaggcc gggattcgcc
    27421 tgagtaaact tccctcatag tggggcgtca gacgccccct taaacattaa aatgtgagca
    27481 ctttatcggc tgacagcgtc cattgcgcca gttccacaag agtaccgatt tccaccccat
    27541 caatcagagg aagtgtgcta atcccgcgcc cgtcggtaca ggttttgcac aatttcaccg
    27601 gtacattctg agcggtaagg atctccagca tttgctgaat gttgtagcct tcccctggtt
    27661 tttgcccgcg caacccggct gtgaccgcat cagacatcag gaacagacgc agatccagat
    27721 tgctctcctg ctctcgtaac gcaatggcca gccgcaagct gttaaacaag gattcgctcc
    27781 cgtaaggtgc gccattggca acgatcacta ttttttgcat tatttactcc tgtattcagg
    27841 gaattagaca ctcatcttct atcttactgc ttctgcagcg tctgaccaat cggtcacatt
    27901 tttaaggatt ttcctgaaag cgcgagaaaa tacgacaaaa gttgccagta atcgttattc
    27961 tttaaggcta tggtttttca ttattaccgg aagttaccga cgttttgagc cgtttcgttc
    28021 ctcgcattat cccgttttat ttactcttgc ttgtagcagg cggtacagct aacgcacaat
    28081 ctaccttcga gcaaaaagcg gcaaatccct ttgataataa caatgatggt ctgccggatt
    28141 taggcatggc tcccgaaaat catgatgggg aaaaacactt tgctgaaatt gtgaaagatt
    28201 tcggcgaaac cagtatgaat gataacgggc tggatactgg cgagcaggca aaagctttcg
    28261 cattgggaaa agtccgcgac gcgcttagtc aacaggttaa tcagcacgta gagtcctggc
    28321 tatcaccgtg gggaaatgcc agtgttgacg tcaaagtgga taacgaagga catttcaccg
    28381 gcagtcgtgg aagctggttt gtgccgttac aagataatga tcgttatctc acctggagcc
    28441 agcttggtct tactcagcag gataatgggt tggtgagcaa tgtgggcgtt gggcaacgct
    28501 gggcgcgcgg caactggctg gtgggttata acacttttta tgacaacttg caggacgaaa
    28561 atcttcagcg agcgggcttt ggtgccgaag cgtggggcga atatttgcga ttatcggcaa
    28621 acttttatca gccgtttgct gcatggcatg aacagacagc cacgcaggaa caacggatgg
    28681 cgcgcgggta cgacctgaca gctcggatgc gcatgccgtt ctatcaacac ctcaatacca
    28741 gtgtcagcct agaacagtat tttggtgatc gtgttgattt gtttaactct ggtacgggtt
    28801 atcacaatcc cgtcgcgttg agtctgggat taaattacac ccctgtgcca ttagtcactg
    28861 tgacggccca gcataaacag ggggaaagtg gcgaaaatca aaataacctc gggctgaatc
    28921 ttaattaccg ctttggtgta ccgctcaaaa aacaactttc tgcgggcgag gttgccgaaa
    28981 gtcagtcgtt acgtggtagt cgctatgaca atccgcagcg aaataatcta ccgactcttg
    29041 agtaccgaca gcgaaaaacg ttaacggtgt ttctggcgac accgccgtgg gatctaaaac
    29101 ctggcgaaac agtgccgctg aaattacaaa tccgcagtcg ttacggtatt cggcaactga
    29161 tttggcaggg cgatacgcag atattaagtt tgacgccggg cgcacaagcc aacagcgcgg
    29221 agggctggac gctgatcatg cctgactggc agaacgggga aggggcaagc aatcactggc
    29281 gattgtctgt ggtggtggaa gataaccagg ggcagcgtgt ctcctccaat gagatcacgc
    29341 taacgcttgt cgaaccgttc gatgcattgt caaacgacga actgcgctgg gaaccgtaat
    29401 cagaaaatgc gctcctgatg cacccatacc gctgcttcca cgcgagactt gagcttcatt
    29461 ttcttcagca tgtgcttgac gtgcactttt actgtgcttt cggtgatatc caggcggcgg
    29521 gcaatcatct tgttcggcaa accctgggca atcagcttga gaatatcgcg ctcgcgtggg
    29581 gttaactggt taacatcgcg ctcagtagtg gcacggttag cgcgcaagct ggcggccaga
    29641 acaggcgtta atgcttcgct taataccatt tcgccagcag ctgcctgatg caatgctttc
    29701 agcagatctt ccggttccat atcttttaac agatagccat ccgcgccgcg tttcagtgcg
    29761 gtgaccacat cttcttcatg gttagagacg ctgaatacca caatgcgccc tgagagggac
    29821 ttttcgcgca gtttatccag cgtttccaga ccgttcatgc cgggcatatt gagatctaac
    29881 aggatcagat cgggatcaag agattccgcc agttcaatac cctgttcgcc attactcgct
    29941 tcgccaacca cggtgatatc tggtgccata ctgataagct gttttacgcc agttcgcagc
    30001 atcgggtgat cgtcaatcag caggatagta gccggttcct gattactcat gggtatctcc
    30061 ttggacgtct gtgaaagttt tttcgggaat aaaggtgacc accacttcgg tgccacctga
    30121 ttcacgacgg cggacgcggc aatcgcctcg taaactttgc gcacggtcgc gcattattat
    30181 catgccgtag tgattgctgc ggatggcatt ttcaggcacg ccacagccgt tatcctggac
    30241 ggtcagtttg acctgattat cgttttgcgc caccgtcacc acgacctcac tcgcttgcga
    30301 atgtttgagg gcgttactta atgcctcacg ggcaatttgc aacaagtgga ttgcctgatg
    30361 tgaaggcacc agacgaggcg gcaattgata atccagcttc accgggaagc caaatttggc
    30421 gctgtactct tcgcaactcg cttccagcgc cggacgtaat ccaggctcgg tgagctgcaa
    30481 gcggaatgtg gtgagcaatt cacgcaactg cgcccaggat gcattcagtt cgttacggat
    30541 ctgacttaac agttcgcggc tgctttctgg cagcgcatcg ccctgcatct gtaaacaact
    30601 cacctgcatc ttcatgcaag agagagattg ggcaatagaa tcatgcagtt cgcgcgcaat
    30661 ggtggcacgc tcttccatca cgatcaactg ttgctgacgt tcctgatggc gatccagcgc
    30721 cagcgtggcg gtaagttgtt caaccagggt atccaccagt tgttgttggt catggctaag
    30781 atgacgcccc tgcggcaggg tcgccagcaa aataccgtac tgcgtatgag agtcagccag
    30841 ccgccacttc agggtcgtgc cgcgatcacc aacgggtaat acgccgcgcg ggcagagctg
    30901 gcagccttta tcatcacaag tcatatctgg ctggcaggta aactcctgat gattctcttc
    30961 atcatcagtg tcatacaccc gcagttcgat atcacgtagc agggttaaat tctgtaagcc
    31021 gttgagtacc ggtgacaggc gttcacacag cggggcgcgg gaatgcaaac ggcggttagc
    31081 ctgccataaa aaagagagga tctgattttt atgctccagc ccggcggttt tctcctgaac
    31141 ccgctgctca agtacggcat aactttcggc cagttctgca gacatattgt tcaacgcagt
    31201 tccaagcatc gccatttcgt tgcgcccgct gatgtttgcg cgttgggtaa aatcgcgatg
    31261 gctgacggca ctcgccattg ccagcagttg ccgccacggt tgtagcagtc gcgcccgcaa
    31321 ccagataata gtgaacacca gtaaaagtgc cataaatacc gccattaccc gatggaccag
    31381 taccactgtc tcaatacgca tttccgtggt gcggtcaaaa ccagatacca gctgatcaag
    31441 cccggcaaca aactggctga catccgctga caccgtttct cggttttgtg cacgcatcag
    31501 cgcagggatc agttcattac gccagtaatc ttgtaagccc tgtaattgcg ccagttgtcc
    31561 gtctcgttct gctgctcgag tcaactcggc gctaaatgcc gtttgttcca tctctttaat
    31621 taagggcttg tctttctcgc ttaatggcac tgccgccaac agacggtaac tttgcatgcg
    31681 cagcgatccc gctttgttga tcgcatgggc gctgccctga acgccttgca ccagccagcc
    31741 agaaaccgcc atccctgcca gtccaatagc agtagaaagc aacacaataa gcgcaacctg
    31801 attaaccagg gtgagcggag agagacaacg tttaagcatg taaacccctt ccttcaggct
    31861 ttaaatgagc aataacctta atgaatgtga cgatatattc tggaatggca gtattctcgg
    31921 ctattggctg aagtataccc atagccggaa agagttactc cttatttgcc gtgtggttag
    31981 tcgctttaca tcggtaaggg tagggattct acagcaccgt gaaaaatctc ataattttta
    32041 tgaaatcact gtactcacta tgggtaatga taaatatcaa tgatagataa agttatctta
    32101 tcgtttgatt tacatcaaat tgcctttagc tccagacact aaggtggcag acatcgaaac
    32161 gagtatcaga ggtgtctatg agtcactcat ctgcctccga aagggctact ggagctgtca
    32221 taacagattg gcgaccggaa gatcctgcgt tctggcaaca acgcggtcaa cgtattgcca
    32281 gccgcaacct gtggatttcc gttccctgtc tgctgctggc gttttgcgta tggatgttgt
    32341 tcagcgctgt tgcggtgaac ttaccgaaag tcggttttaa ttttacgacc gatcagctat
    32401 ttatgttgac tgcgctgcct tcggtttctg gcgcgttatt acgtgttcca tactccttta
    32461 tggttcctat cttcggtggt cgtcgctgga cggcgttcag caccggtatt ctgattattc
    32521 cttgcgtctg gctgggtttt gccgtgcagg atacctccac gccttatagc gtcttcatca
    32581 tcatctctct gctatgcggc tttgctggcg cgaacttcgc atccagtatg gcaaacatca
    32641 gcttcttctt tccgaaacag aagcagggtg gcgcgctggg tctgaatggt ggtctgggaa
    32701 acatgggcgt cagcgtcatg cagttggttg ctccgctggt ggtatcactg tcgattttcg
    32761 cagtatttgg tagccagggc gtcaaacagc cggatgggac tgagctgtat ctggcgaatg
    32821 cgtcctggat atgggtgccg ttccttgcca tcttcaccat tgcggcgtgg tttggcatga
    32881 acgatcttgc tacctcgaaa gcctccatca aggagcagtt gccggtactc aaacggggtc
    32941 atctgtggat tatgagcctg ctgtatctgg caaccttcgg ctccttcatc ggcttctccg
    33001 cgggctttgc gatgctgtca aaaacgcagt tcccggatgt tcagattctg caatacgctt
    33061 tcttcgggcc gtttattggt gcgctggcgc gttctgcagg tggtgcatta tctgaccgtc
    33121 tgggcggaac tcgtgtcacg ctggtgaact ttattctgat ggcgattttc agcggcctgc
    33181 tgttcctgac cttaccgact gacgggcagg gcggaagctt catggcgttc ttcgcggtct
    33241 tcctggcgct gttcctgaca gctgggctgg gtagtggttc cactttccag atgatttcag
    33301 tgatcttccg taaactgaca atggatcgcg tgaaagcaga agggggttct gacgaacgtg
    33361 cgatgcgtga agcggcaacc ggcacggcag cggcgctggg tttcatctct gcgattggcg
    33421 cgattggtgg cttctttatc ccgaaagcgt ttggtagctc gctggcatta acgggttcgc
    33481 cagtaggtgc aatgaaagta tttttgattt tctatatcgc ctgcgtagtg attacttggg
    33541 cggtatatgg tcggcattct aaaaaataaa tcttaatatt tgattatcct ttgcgcggca
    33601 tgatgccgcg ctttttttat gcgtcattta gttacaacat attagtgtta tatggcttat
    33661 ttcgccggat ttcattaaga gccattaata tgttaccc
//
LOCUS       DAZOZX010000032        32261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZOZX010000032 DAZOZX010000000
VERSION     DAZOZX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.032"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..211)
                     /locus_tag="ACHUPT_001487"
     CDS             complement(<1..211)
                     /locus_tag="ACHUPT_001487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819111.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFVFIFTLKLRICGFIFNKIITTG"
     gene            complement(236..2871)
                     /gene="mngB"
                     /locus_tag="ACHUPT_001488"
                     /pseudo
     CDS             complement(236..2871)
                     /gene="mngB"
                     /locus_tag="ACHUPT_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(2889..4805)
                     /gene="mngA"
                     /locus_tag="ACHUPT_001489"
     CDS             complement(2889..4805)
                     /gene="mngA"
                     /locus_tag="ACHUPT_001489"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0819112.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            4975..5697
                     /gene="mngR"
                     /locus_tag="ACHUPT_001490"
     CDS             4975..5697
                     /gene="mngR"
                     /locus_tag="ACHUPT_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0819113.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5800..6669)
                     /gene="sucD"
                     /locus_tag="ACHUPT_001491"
     CDS             complement(5800..6669)
                     /gene="sucD"
                     /locus_tag="ACHUPT_001491"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0819114.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6669..7835)
                     /gene="sucC"
                     /locus_tag="ACHUPT_001492"
     CDS             complement(6669..7835)
                     /gene="sucC"
                     /locus_tag="ACHUPT_001492"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0819115.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7929..9146)
                     /gene="odhB"
                     /locus_tag="ACHUPT_001493"
     CDS             complement(7929..9146)
                     /gene="odhB"
                     /locus_tag="ACHUPT_001493"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0819116.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(9161..11962)
                     /gene="sucA"
                     /locus_tag="ACHUPT_001494"
     CDS             complement(9161..11962)
                     /gene="sucA"
                     /locus_tag="ACHUPT_001494"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0819117.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12263..12979)
                     /gene="sdhB"
                     /locus_tag="ACHUPT_001495"
     CDS             complement(12263..12979)
                     /gene="sdhB"
                     /locus_tag="ACHUPT_001495"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0819118.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12995..14761)
                     /gene="sdhA"
                     /locus_tag="ACHUPT_001496"
     CDS             complement(12995..14761)
                     /gene="sdhA"
                     /locus_tag="ACHUPT_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0819119.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14761..15108)
                     /gene="sdhD"
                     /locus_tag="ACHUPT_001497"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14761..15108)
                     /gene="sdhD"
                     /locus_tag="ACHUPT_001497"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0819120.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15102..15506)
                     /gene="sdhC"
                     /locus_tag="ACHUPT_001498"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15102..15506)
                     /gene="sdhC"
                     /locus_tag="ACHUPT_001498"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0819121.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15887..15994
                     /locus_tag="ACHUPT_001499"
     CDS             15887..15994
                     /locus_tag="ACHUPT_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819122.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16200..17483
                     /gene="gltA"
                     /locus_tag="ACHUPT_001500"
     CDS             16200..17483
                     /gene="gltA"
                     /locus_tag="ACHUPT_001500"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0819123.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            17873..18439
                     /locus_tag="ACHUPT_001501"
     CDS             17873..18439
                     /locus_tag="ACHUPT_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0819124.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            18490..20949
                     /locus_tag="ACHUPT_001502"
     CDS             18490..20949
                     /locus_tag="ACHUPT_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0819125.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DGNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            20964..21695
                     /locus_tag="ACHUPT_001503"
     CDS             20964..21695
                     /locus_tag="ACHUPT_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0819126.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            21692..22768
                     /locus_tag="ACHUPT_001504"
                     /pseudo
     CDS             21692..22768
                     /locus_tag="ACHUPT_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            22920..23966
                     /locus_tag="ACHUPT_001505"
     CDS             22920..23966
                     /locus_tag="ACHUPT_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0819127.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(23963..24754)
                     /gene="nei"
                     /locus_tag="ACHUPT_001506"
     CDS             complement(23963..24754)
                     /gene="nei"
                     /locus_tag="ACHUPT_001506"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0819128.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(24790..25524)
                     /gene="pxpA"
                     /locus_tag="ACHUPT_001507"
     CDS             complement(24790..25524)
                     /gene="pxpA"
                     /locus_tag="ACHUPT_001507"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0819129.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(25514..26446)
                     /gene="pxpC"
                     /locus_tag="ACHUPT_001508"
                     /pseudo
     CDS             complement(25514..26446)
                     /gene="pxpC"
                     /locus_tag="ACHUPT_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(26440..27096)
                     /gene="pxpB"
                     /locus_tag="ACHUPT_001509"
     CDS             complement(26440..27096)
                     /gene="pxpB"
                     /locus_tag="ACHUPT_001509"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0819130.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(27119..27862)
                     /gene="ybgI"
                     /locus_tag="ACHUPT_001510"
     CDS             complement(27119..27862)
                     /gene="ybgI"
                     /locus_tag="ACHUPT_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0819131.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            28133..29614
                     /gene="dtpD"
                     /locus_tag="ACHUPT_001511"
     CDS             28133..29614
                     /gene="dtpD"
                     /locus_tag="ACHUPT_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0819132.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(29764..31182)
                     /gene="phrB"
                     /locus_tag="ACHUPT_001512"
     CDS             complement(29764..31182)
                     /gene="phrB"
                     /locus_tag="ACHUPT_001512"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR0819133.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(31179..31688)
                     /locus_tag="ACHUPT_001513"
     CDS             complement(31179..31688)
                     /locus_tag="ACHUPT_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0819134.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            31817..32104
                     /locus_tag="ACHUPT_001514"
     CDS             31817..32104
                     /locus_tag="ACHUPT_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR0819135.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
BASE COUNT         7772 a         8676 c         7920 g         7893 t
ORIGIN      
        1 cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg agagtgaaga
       61 taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct ctgcaatata
      121 ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg gcatgaacaa
      181 agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt gctattcagg
      241 caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat agatttgaac
      301 cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa gaaatcacct
      361 cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta aacagccgta
      421 aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggggc atcccactgg
      481 gggcattttc aacaaactat aactttccgg cacgttgaat ccggctttgt tgagcttcat
      541 cgcatcccat gggattttgt tgtaacactg tactggagtc agccatgctc gcgcctgctg
      601 agctacacca gcggcggttg gcgtaccggt ataactcaat aaacttaggc gacaagaaag
      661 cagaccacgt agttgtgagt ccgggactgg cattttaatt cccgaaggcc gcctaggcct
      721 taaaagcaga tcttctttcc ccagtaagcc cacgccgcgc agcaacgtaa tggcaaaggt
      781 tttcttctct tcaccgatga cttcaaattc acgtagccct tcgctaaaga cagccatgcc
      841 gttacgccct tcctgtaagg ctgcatagtt gagcatattc cataccggaa ccggcgcttc
      901 tttccagctt tcttgctgcc agttgttcat tgcactgtcg ttcacggggc gcgttagcga
      961 accaaactgg gtatctgcca gaacactatc ggtgttaaaa gatgtaggga tcaggacgcg
     1021 aaggcgatga tcgtcagcct ggttatcaag gttgatatcc acatcaatgc gcctgttgtt
     1081 atgactaaga gtgacaacca actcgacatc tactctgcca gtggattgtc ttgcgctgcg
     1141 ttgttgcaga ttacgcggca ccgccatttc atagcggata acagccctgc tctgccaggc
     1201 ctcatgaata atctcgcatt gcggtttcgc attcgccgaa gtaattaccc actcttcttt
     1261 tgcgggtgaa tagtcatatt catcaccatc atcagagctt tcttcaattt gcaacacccg
     1321 gtcatagcgc acaccgctat ctttatctac cagttgtagc gttccatcct cattgagcgc
     1381 aatttgccag aaagcatttt ccagtatccc ttcagcgtca ctttttgccg caatcacgtt
     1441 gccaggctga ttcgcttcga tataaagcgt gcgatagccc atagaaggga caatctggtt
     1501 gatctgtata tcaaactcca taaagggatc gtaattaccg taatgaacta tttgccgatc
     1561 gattaggcct ggatcgatct cacgggcatg gcgaataaaa tacggtacag gctgaccgcg
     1621 atcgtctcgt agattaaact ggctggcgcg cagccgcaca gtggtgttga tcacttcttc
     1681 ccgcggccag ggcatcaggt taaacaggac gagtttgtcg gcgtcgctct gcggcatgtt
     1741 gtcggcgatt ttacgcatat agaaacgcag cagattatcc gccatgtctt cagccagttc
     1801 gaagcgggcg acgatttcgc gatgaacttt gtcacgacag cagcagccga tgctgtcgtg
     1861 ggcatgattt tttaagatct ctttccacat tttctccagc aagccgtggt ggtatccaaa
     1921 acccaacgtc ctggccagcg ttgccagcgg ttccagcaga ttaacaatct tattttcaat
     1981 acgtgcgtgg gcaactttga tatccatgcg cgtggaaccg atggtgcgat gcacgcgcat
     2041 atatttgcca tcaataaatt cccctttcag ggttgccaga ttatctcgct gcgcttcgat
     2101 cttctcaaat acctcttcaa agcggctcat cacaaacttt cgttgaggat aaatttcgcg
     2161 cagtttctcc atcacttcga agatattttg ctgcaatggc atctgatcat gcccattcgg
     2221 cagcaaaatc tctttggtta cagacgcttt ttccagcacg tcaaaataac tgtcgaggcg
     2281 tttgcgtaat ccgttttcgt ccgcaggtaa gtacttaccg atggcataac ccaacggcat
     2341 cacctgcgcc gtcacttcgc taccgtcact gctttgccac aaaaactcgg ttttatccgt
     2401 accgtggcgc tcagaacatc cacgccagaa catggtgcag gtaatgccaa atccattgta
     2461 gatatgcggc agttgcccgg acatgccaaa ggaatcgggt aaataaccta ttttcatcgg
     2521 ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga ttacggacga tggattccgc
     2581 agaaacaatc gtggtatcgg tctgggtata ccaggggccg ataatcaact tgccggcttc
     2641 taccagtttc ttcaagcggt ctttgttttc cggtttcacc gcgaaataat cttcgaggat
     2701 cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg tcctgttcca gtcggcacag
     2761 gatctcttcc atattattga ccagcagaat gcgtgactct tcggtggtga aataccactc
     2821 ccgatcccag tgcatatgcg gggtgatgtg aacgcgagat accgctttca tcgtcgtttc
     2881 ctttttgttt atggcattac gccatcagtc agatagttgc catgcttaac cgcgtgacgc
     2941 cgccagatca ggagaattgc agtcgagatt gcagccccca ccaatgccgc accaaaccag
     3001 ccaattgccg ccataacacc gcccgcgcca ttatcatgaa gtaaaaagag cgagaaaatg
     3061 ccagcaccgg gtgtcgaaag gccgatattc atcgcaccga caatagcgcc cgttaccata
     3121 gagcccagca caaacgaacc aataacccgc agcggatctt caatcgccat cgggatcgcc
     3181 ccttcggtaa tacctgccag ccctaacagc caggtggatt tcccggtctc aatttcaaac
     3241 tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta cggtaaatgc cgaaaccatt
     3301 ttgacggagg cgaaaatggc atacgggccg taaacgccgt tcgccattgc gcccaggcag
     3361 aatgcataag cggctttatt cactggccct ccaaggtcaa aggaacacat aaaaccgaga
     3421 atggcaccca gcaacagcgc gttacttcct gacagaccgt tcagccaggc ggtaagcgag
     3481 ttattgatcc aggcgacagg ttcccccacc acaaacagca tcagactgcc cgctcccaat
     3541 gtaccgagca ccgggtagag ataaaaagtc aggaagccat tgaatttact gctaagacgc
     3601 aagtgatttt tcatccagcg tatcaagtaa ccggctatca atccgccaac gaccgcgccg
     3661 agaaacccgg agccgatcat gttggcggca agtccagccg caaagcctgg cgctaacgcc
     3721 ggtttatctg ccagcgaata ggcggtatag gctgccagca ccggtaccat caaaattccg
     3781 agcagcccgc cgcccagttt gcggtacatc cacagccagg agttttcttc attaaacaga
     3841 tcttgtagcc cgaagatttg cgacagtaat accgcgaccg ccagcaccgt cccccccgcg
     3901 acaatcaacg gtacggcaaa agagatcccg ctcaacagtg cctgtttcag ctccgttttg
     3961 acgcttttca ccggttgcgt atcttgctgt aaggtacgcg tctcatcgcc acgctcgagg
     4021 gtaagcgatt gttggatcaa cgcttctgca tggcgaatcg gctcggcaac aggcacagaa
     4081 agcgcaggaa tgccgttaaa acgctcactc tccttgatgg cgacttcagc cgcaaaaata
     4141 caggcagttg cactattgag ttgatccgcc gttaaacgcc cttcaatacc gttagcacct
     4201 tgcttttcaa cgtagacatt cacgccgagt ttgcgtccgg ctttttccag atactccgca
     4261 gccatgtagg tgtgagcaat acccgccggg caggccgtta cgcaaacgat agttggcgcg
     4321 ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat ccagcgccga gagcaattca
     4381 tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat catccgccag gcgcgtggtc
     4441 agcgctgtca gcagttgcat gtgcgtagta cccgcttcat tgaggggaat cgccagcaac
     4501 accactaaat caaccgcttc cgggccatcc acgccttccc actgaagcgg ctcgctgagt
     4561 gtggcgactg caaacgccgc ttcttttacc gcagcagttt tgccatgcgg cacagccaac
     4621 ccttcactta aggcggtcgg gccaaggctt tcacggcgat acacttcttt cagaaattgc
     4681 tcagtactgg aaattttccc cagagcagca agacgttgag tcaacgcgtg aatggcctct
     4741 tcacggctgg taaagcgcgc attcagacac aacgcatcgc ggtgggtcag agtcgtcagg
     4801 ttcatcatga tcctcacttg atcgttttta tagtcgcgcc agtgtgcccg acaaaacaat
     4861 accattgcgt aatcaatttc acttgattga aattatttgc attttatttg tattgtttac
     4921 aggatgagtt tatcgggcat aatggctaaa ttcgtattaa tgagatgcta aataatggga
     4981 cacaaaccct tataccgaca gattgctgat cgcattcgtg agcaaattgc gcgtggcgag
     5041 cttaaacctg gcgatgcgtt acctaccgaa tcggcattac aaacagagtt tggcgtcagc
     5101 cgggttacgg tgcgtcaggc tttgcgccag ttagtcgagc aacagatcct cgaaagcatt
     5161 cagggcagcg ggacttacgt caaagaagag cgggtcaatt acgatatttt tcagttaacc
     5221 agttttgatg aaaaactgtc ggaccgtcac gtcgatacac acagtgaagt tctgatattt
     5281 gaagtgattc cggctgacga ttttcttcag caacagctac aaatcaccgt gcaggatcgt
     5341 gtttggcatg tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct ggaagaaacc
     5401 tggatgccgc ttgcgttgtt cccggatctc acctggcagg tcatggaaaa ttcgaaatat
     5461 cactttattg aagaagtgaa gaagatggtt atcgatcgta gcgaacagga aatcattcct
     5521 ctgatgccaa ccgaagagat gagccgtctg cttaatatca gccagacaaa gccgattctt
     5581 gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt ttgaatatag ccgcaacgct
     5641 tttaataccg atgactataa atttaccctg atagcgcagc gaaaatcatc gcgataagca
     5701 caaaaaggcc atcatacgat ggccaaccat gtcgaaaccg acagcccttc gccattcctg
     5761 tagagggaag cggcgagggc tatttcttat tacagatatt tatttcagaa cagttttcag
     5821 tgcttcaccg atatccgcca ggctgcgaac ggttttcacg cctgcggctt ccagagcagc
     5881 gaatttctca tccgcagtcc ctttcccacc ggcaatgatc gcacccgcgt ggcccatacg
     5941 tttgcctttc ggcgcagtca caccagcgat gtaacccaca actggcttgg taacatgctc
     6001 tttgatgtac gctgccgctt cttcttcagc gctaccgccg atctcaccga tcatcacgat
     6061 cgcttcggtc tgcggatctt tttcgaacat ttcgagaatg tcgataaagt tagagccagg
     6121 gatcgggtca ccgccgatac cgacacaggt cgactgaccg aaaccgtaat ccgtggtctg
     6181 tttaaccgct tcataggtca gtgtaccgga acgggaaacg atacccactt tacccggttt
     6241 gtgaatgtga cccggctgga taccaatttt gcattcaccc ggggtgataa cgcctgggca
     6301 gttcgggccg atcatacgaa cgcctgcttc gtccagtttc actttcacgg tcagcatatc
     6361 cagcgtcggg atgccttcag tgatggtgat aatcagtttg atgcctgcat cgatagcttc
     6421 cagaatggag tctttgcaga acggtgctgg tacgtaaata acagacgcgg aagcgccagt
     6481 agcagcaaca gcttcacgca cggtgttgaa caccggcagg ccgaggtggg tagtgccgcc
     6541 tttacctgga gttacgccac caaccatttt agtgccgtat gcaatggcct gttctgagtg
     6601 gaaagtcccc tggctaccgg taaagccctg gcagataacc ttggtgtttt tatcaattaa
     6661 aatggacatt atttcccctc cactgcggca acaacctgct gagctgcatc cgtcagacct
     6721 tttgctgcaa taatattcag gccgctgtca gccagtttct tcgcgccgag ttcggcgttg
     6781 ttaccttcca gacgtaccac gaccggtacg ttaacaccca cttccgctac cgcgccgatg
     6841 ataccgtcag cgatcaggtc gcaacgaacg ataccgccga agatgttaac cagaacggct
     6901 ttcactttgt cgtcagagag gatgattttg aacgcttcgg ttacacgttc tttggttgcg
     6961 ccgccgccaa cgtccaggaa gttagccggt tcgccgccgt gcagtttaac tatgtccatc
     7021 gtacccatcg ccagacctgc gccgttaacc atacaaccga tgttaccgtc cagcgcaacg
     7081 tagttcagtt cccactgtgc agcctgtgct tcacgcggat cttcctgcga ctggtcacgc
     7141 atttcgcgca gatcaggctg gcggaacagg gcgttgccat cagcgcccag tttgccgtcg
     7201 aggcaaatca gatcgccctg tttggtgatg accagcgggt tgatttcgat cagcgccagg
     7261 tcgcgctcca ggaaaatggt cgccaggccc atgaagattt tggtgaactg ctgaaccagt
     7321 ttaccttcca gacccagttt gaacgccagc tcgcgtccct gatacggcat cgggccagtc
     7381 agcggatcaa gcgcaacttt atggatcagg tgcggagttt cttccgccac tttttcgatt
     7441 tccacgccgc cttcggtgga ggccataaag accacgcgac gggaactacg gtctacaacg
     7501 gcaccgagat acagctcttt agcgatatcg gtcgccgctt caaccagaat ctggttaacc
     7561 ggttggccat tggcatctgt ttgatacgtt accagacgct tgcccagcca gttttctgca
     7621 aaagcacgga tgtcttcttt gctgtttaca actttcacac cgcccgcttt accgcggcca
     7681 ccagcgtgaa cctgacattt cactacccac ggaccggcac cgatttttga agcggcttct
     7741 tctgcttcgc gcggagtagt acaggcataa cccaccggtg ctggtaagcc atagcgggca
     7801 aacagttgtt ttgcctgata ttcatgtaag ttcatgtgtt ctgtccatcc ttcagtaatc
     7861 gttatctttt aaaccgtaga ccgacaccct atccacgtcg gtctacagtg caggtgaagc
     7921 ttaaactact acacgtccag cagcagacgc gtcggctctt ccagtaactc tttgatcgtt
     7981 accaggaagc ccacggattc gcgaccatcg atcagacggt gatcgtagga cagcgccagg
     8041 tacatcatcg gcaggatctc aacctgacca ttcaccgcca tcggacgatc tttgatagcg
     8101 tgcataccca gaattgcgct ctgcggcggg ttgatgatcg gcgtagacat cagggaaccg
     8161 aacacaccac cgttggtgat ggtgaagtta ccaccggtca gatcttcaac cgtcagcttg
     8221 ccatcacggc ctttgactgc cagctctttg attttcttct cgatgtctgc catgccgagg
     8281 gtatcgacat cacgcagaac cggcgtcacc aggccgcgcg gcgtagaaac cgccatgctg
     8341 acgtcgaaat agttgtggta aaccacgtca tcgccgtcga tagacgcgtt cacttccggg
     8401 taacgtttca gggcttcaac caccgctttc acgtagaagg acataaagcc cagacggatg
     8461 ccgtggcgtt tttcaaacgc ttcaccgtac tgcttacgca gatccataat cggcttcatg
     8521 ttgacttcgt tgaacgtggt cagcatggcg gtggagtttt tcgcttccag cagacgctct
     8581 gccacacgct tacgcaggcg agtcatcggg acacgttttt cactacgtgc agccagagcc
     8641 ggttgcgccg ccggagcagc cgctgccgga gcagactctt tcgccggggc tttcgccaga
     8701 tgtttttcca catcttcacg agtcagacga ccacccacac cggtgccttt aatggcgctg
     8761 gcgtcgagat tgtgttcagc cagcaggcga cggatcgccg ggcttaacgc atcgttgttt
     8821 tgctcttcca gagacgcctg ctggcgttgc gccggagtgg acgctttctc ttcagatttg
     8881 gcgctggttt ctttaccggc gctgttgcct tcacgcaggc gaccaaggat ctgacgagac
     8941 gttaccgttg taccttcatc ttccagaacc gcatccagaa tgccgtctgc tgatgccggt
     9001 acttccagta ccactttgtc agtttcgatt tctaccagca cttcatcacg tacgactgcg
     9061 tcgccgggtt ttttatgcca ggttgcgacg gtggcatcgg ctacggattc aggcaggtca
     9121 gggaccagaa tatctacgct actcattgtg tatcctttat ttattcgacg ttcagcgcgt
     9181 cattaaccag atcttgttgc tgtttctggt gaacggacat ataccctacc gccggagagg
     9241 cggaggccgg gcggcctgca taacgcagag aagccccaaa cggaatcact tcacggaaat
     9301 gatgctggct gcagtaccat gcgccctggt tgagcggctc ttcctggcac cagacaaaat
     9361 ccttgacgtg agcaaactgc tgcaacactt cctgcatcgc tttatgcggg aacgggtaga
     9421 gttgctcgat acgcacaatg gcgacatcgt gttgattgtt cttacgacgc tgttccagca
     9481 ggtcgtaata aaccttacca gaacacatca ctacgcgctt cacgcccttc ggatcaagct
     9541 cgtcgatttc accgatggct ggcaggaagg tgccgttcgc cagttcttcg aggctggaaa
     9601 ccgccagcgg atgacgcagc agggatttcg gcgacatcac gaccagcgga cgacgcatcc
     9661 cgcgcagcgc ctgacgacgc agcatgtggt aaacctgtgc cggggtagac ggtacgcaaa
     9721 cctgcatgtt ttgctcagca caaagttgca gataacgttc cagacgcgcg gaggagtgct
     9781 ccggcccctg cccttcgtaa ccgtgcggca gcaacatcac cagaccacac atccggcccc
     9841 atttctgctc gccagaggag atgaactggt cgataaccac ctgcgcaccg ttggcgaagt
     9901 caccgaactg cgcttcccag atggtcaggg tgcgtggttc tgcggtggca taaccatatt
     9961 caaacgccag cacggcttct tctgacagta cggagtccca gacacggaac gcgccctgac
    10021 cgttatggat gtgttgcagc ggcgtgtaag tggaaccgtt agactggttg tggatcaccg
    10081 cgtggcggtg gaagaaggta ccgcgaccgg agtcttcacc cgacaggcga accggaatgc
    10141 cttcatcaac cagcgtggcg taagcgaggt tttccgcacc gccccagtcg aacagtttct
    10201 caccggctgc catcgcctgg cgatcgccat aaatcttggc aacgcgagac tgcatttcaa
    10261 ctgcttctgg taccgtactg atacgtttag ccagttcctg caggcgcttc atctcaactt
    10321 tgttcgggta ctcttcgtcc cattcgtggt tgaggtacgg cgaccaggtg aaagagtgca
    10381 tgttcatcgg acgccactct gccactacgc aatcgccagc atccagcgca tcgcggtaca
    10441 ggttaaccat ctcggtggca tcttccagcg tcgccacttt ttcctgctcc agcttgtcag
    10501 cgtagatttt gcgcggtgtc ggatgttttt tgattttctg atacatcagt ggctgggttg
    10561 cgctcggctc gtcggcttcg ttgtggccgt gacggcggta gcataccagg tcgatgaaga
    10621 catcacgttt aaaggtgtta cggaaatcga gcgccagacg ggtcacaaag gcaacggctt
    10681 ccggatcatc cgcgttaacg tggaaaatcg gtgcctgaac catcttaccg atatcagtac
    10741 agtacggtgt agaacgggca tccagcgggt tagaggtggt gaaaccaacc tggttgttga
    10801 taacgatacg taccgtaccg ccaacttcat aaccacgcgc tttcgacatg ttcagagttt
    10861 cctgaaccac gccctgcccg gtcactgcgg cgtcaccgtg gatggtgatt ggcagcactt
    10921 tgttgctgtt cggctcatca agtctgtcca gacgggcacg aacagagccg ataacgaccg
    10981 ggcttacaat ctcaaggtga gacgggttaa acgccagcgc caggtgcacc aggccgccat
    11041 cggtctggaa gtcagacgag aagcccatgt ggtatttcac gtcgcctgtg ccgaggtgtt
    11101 ctttatgttt accggcgaac tcgtcgaaca agtcttgcgg ttttttaccc agcacgttca
    11161 ccagcacgtt cagacgacca cggtgcgcca tcccgagaac cacttcgcgg gtgccgctgt
    11221 tgccagcgtg gcggatcatc tctttaagca tcgggattaa cgcgtcaccg ccttccagcg
    11281 agaagcgttt tgcgccaggg aattttgcgc cgaggtaacg ctcaaggcct tcagcggcgg
    11341 tcagttcgct taagaagcgt tttttctctt cgctattgaa agtcgcgcga ccagactcga
    11401 tacgctgttg gatccagcgt ttttcttcgg tgctggtgat gtgcatatac tcggcaccaa
    11461 tcgggccgca gtaggtttgc ttgagggcgg aaataagctc gccgagtttc atggtttctt
    11521 tgccgctggc aaatgaaccg acgttgaagg tttcctggaa gtctgcttcg gtcagatcgt
    11581 ggaaagacgg atccagatcg gccactttat cttgctgcca cagtcccagc ggatctagat
    11641 tcgcatgctg gtgaccacgg aagcggtatg cgttaatgag ctgcaggact ttaacctgct
    11701 tcacattggt gtcagggtcg gagatcgttg aagagtaacg tgaagcgtct ttcgccaggc
    11761 ggcggaaata ttcacgcgtt tgagagtgga attgatccgg tttgactccc gtaccaggta
    11821 actgctggaa cgtcgaacgc cagttagcgt caaccgagtc aggatcggtt aagaagtctt
    11881 catagagctg ttctatccag ctctggtttg cgccagagag gtaagaagag tccaaccagg
    11941 ctttcaaagc gctgttctgc atcgtgatcc cttaagcatc ttttttatgc ttacttcgcc
    12001 gtggatacta ccacgcacag tgatgtgcgt gccggggttc acttgcgagc tcttgtctac
    12061 gtagtggctc gcgaaggaac ctttaaaaac tgtcttagtg tcaggggcag tttttaaaga
    12121 tttcctgcat ttctttgtta cgcctgatgc gctgcgctta tcaggcctgc gggtaatgac
    12181 ttgtaggccg gataaggcgt tcacgccgca tccggcactg gttgcctgat gcaacgcttg
    12241 cgcgtcttat caggcctaca gtttacgcat tacgttgcaa caacatcgac ttgatatggc
    12301 cgatggcgcg cgtcgggttc agccccttcg gacatacact gacgcagttc atgatgctgt
    12361 gacagcggaa tacgctgaat gcatcgctca aaccgtcgag gcggctgtca gtctcggtat
    12421 cacggctatc gatcaggaaa cgatatgccg ctaacaagcc tgccgggccg ataaacttat
    12481 cgggattcca ccagaaagac ggacaagagg ttgaacaaca tgcgcagaga atacattcat
    12541 acagcccgtc gagtttttcg cgctgctctg gcatctgtaa atgctcgcga gctggcggat
    12601 tttgtccatt attcaacagg taaggcttaa ttttctcata ttgcgcatag aattgtccca
    12661 tgtctaccac caaatcgcgg atcaccggta aacctggcag cgggcgaatc acaatcttct
    12721 tgcccggctg gttgagtgcc gaaatcgggg taatacaggc cagaccattc ttgccgttca
    12781 tgttcagacc gtcggaaccg cacacacctt cacggcagga gcggcggaac gacaggctgg
    12841 gatctttctc tttcagctgg ataagcgcat ccagcagcat catgtcgcga ccttcttccg
    12901 cttccagggt gtaatcctgc atacgcggag catcatcaac atccgggtta tagcgataaa
    12961 ttgaaaactc gagtctcatt ttcctgtctc cgcattagta agtacgaatc ttcggcggga
    13021 atgccgggcg cagtttcggt tccatgttga cgcttcggcg cgtcatggat tccgactcag
    13081 gcagatacag ggagtggcac agccagtttt catcatcacg atccgggaag tcgaagcggc
    13141 tatgtgcgcc acggctttcg gtacggaagt tggcagaaac agccgttgca tacgccgttt
    13201 ccatcaggtt atccagttcc aggcactcaa cgcgctgggt gttgaactcg ctggaagtgt
    13261 catccagacg ggcatttttc agacgctcgc ggatcacttt caactgctca agccctttcg
    13321 ccatcgcatc accttcacgg aagaccgaga agttatgctg catacattct tgcagcgctt
    13381 tacggatcgc caccggatct tcaccgttac gattattgtt ccaccggttc aggcgatcca
    13441 gagacgcttc aacatcagac tcgctggcat cgtgcagtgc gccctgctcg gcgatagact
    13501 cttgcagatg cagacctgcc gcgcgaccaa agaccaccag gtccagcagc gagttgccgc
    13561 ccagacggtt agcgccgtgt accgatacac aagcgatttc accaacggca aacagtcccg
    13621 gaacaaccac atcttcgcct ttctcattca cagtcagcgc ctgaccggta actttggtcg
    13681 gaataccgcc catcatgtag tgacaggttg ggataaccgg aatcggctct ttcaccggat
    13741 cgacgtgagc gaaggtacgg gaaagctcca ggatacccgg cagacgggat tcgagaactt
    13801 ctttacccag gtgatcgagt ttcagtttcg cgtgtggccc ccacggacca tcacagccgc
    13861 gaccttcacg gatttcaatc atgatggaac gcgccaccac atcacggccc gccaggtctt
    13921 tggcgttcgg cgcataacgc tccataaagc gttcgccatg tttgttcagc agataaccgc
    13981 cttcaccacg gcaaccttcg gtgaccagta cgcccgcacc ggcaatgccg gtcgggtgga
    14041 attgccacat ttccatatcc tgcaccggta cgcctgcgcg gatagccatg ccgacaccgt
    14101 cgccagtgtt aatgtgggcg ttagtggtgg actgataaat acgccctgct ccgccagtcg
    14161 ccagcacggt agcgcgggct ttgaaataaa ccacttcacc ggtttcgatg cacagtgcgg
    14221 tacaacccac caccgcgcca tcctggtttt tcaccagatc cagcgcatac cactcggaga
    14281 aaatggtggt gtggtttttc aggttctgct gataaagcgt gtgcaacagt gcgtgaccgg
    14341 tacggtcagc agccgccgca gtgcgtgccg cctgctcgcc gccgaagttt ttcgactgac
    14401 cgccaaacgg acgttgatag atacgaccat catcgagacg cgagaacggc aggcccatgt
    14461 gttcgagttc cagaatcgct tccggcccgg ttttacacat atattcaatc gcgtcctggt
    14521 caccgatata gtccgaccct tttacggtgt cgtacatatg ccattcccag ttatcttcat
    14581 gggtattacc cagcgcaacg gtaatgccgc cttgcgcaga aacggtatgg gaacgggtcg
    14641 ggaagacttt agagagcagg gcacaggtct ggccgctctg ggaaatttgc agcgccgcgc
    14701 gcatacctgc gccaccggca ccaatcacaa ctgcatcaaa ttctctgact ggcaatttca
    14761 tcacacaccc cacaccacaa cgaatccata aatcacgtaa accaccagtg caacgacaat
    14821 caccagttgc agcatcaggc gcaaggccag cggtttaacg tagtcggtca acacctgcca
    14881 catgccgatc caggcatgaa tcaagataga aaacagcgcc agcagggtga acactttggt
    14941 gaacgcagag gcgaagaaac cgatccagac ttcatatgtc agttcgccac tggtggcgaa
    15001 aaaaccgacc atataaatga tgtagagcgt caggacgata gcggtagcgc gaacgaggat
    15061 gaaatcatgt acgccattgc gtcctaatgc ggaggcgttg cttaccatac gaggactcct
    15121 gcgagaagtg aaagcacgac agtaataaca aaggagattt tggcggagcg tttacccgct
    15181 tcgaatgttt cttccagata gccaaaatcc atcatcatgt ggcgaatacc tacgacgacg
    15241 tgatacgcca gagcggtaag gatgccccac atgataaatt tgacgaagaa gctgcccata
    15301 atcgcggaag cttgctcgaa accttcaggg gaagagaggc tggtacccag aagccactgc
    15361 aggatgccca ctgcaacaaa ggtgatcaca ccggaaacgc gatggagaat ggacgctatc
    15421 gccgtgatgg ggaaccggat ggtctgtagg tccagattaa caggtctttg ttttttcaca
    15481 tttcttatca tgaataacgc ccacatgctg ttcttattat tccctgggga ctacgggcac
    15541 agaggttaac tttctgttac ctggagacgt cgggatttcc ttcctccggt ctgcttgcgg
    15601 gtcagacagc gtcctttcca taactgcgcg tcatgcaaaa cactgcttcc agatgcgaaa
    15661 acgacacgtt acaacgctgg gtggctcggg attgcagggt gttccggaga cctggcggca
    15721 gtataggctg ttcacaaaat cattacaatt aacctacata tagtttgtcg ggttttatcc
    15781 tgaacagtga tccaggtcac gataacaaca tttatttaat ttttaatcat ctaatttgac
    15841 aatcattcaa caaagttgtt acaaacatta ccaggaaaag catataatgc gtaaaagtta
    15901 tgaagtcggt atttcaccta agattaactt atgtaacagt gtggaagtat tgaccaattc
    15961 attcgggaca gttattagtg gtagacaagt ttaataattc ggattgctaa gtacttgatt
    16021 cgccatttat tcgtcatcaa tggattcttt acctgcaagc gcccagagct ctgtacccag
    16081 gttttcccct ctttcacaga gcggcgagcc aaataacaaa cgggtaaagc caggttgatg
    16141 tgcgaaggca aatttaagtt ccggcagtct tacgcaataa ggcgctaagg agaccttaaa
    16201 tggctgatac aaaagcaaaa ctcaacctca acggggacac agctgttgaa ctggatgtgc
    16261 tgaaaggcac gctgggtcaa gatgttattg atatccgtac tctcggttca aaaggtgtgt
    16321 tcacctttga cccaggcttc acttcaaccg catcctgcga atctaaaatt acttttattg
    16381 atggtgatga aggtattttg ctgcaccgcg gtttcccgat cgatcagctg gcgaccgatt
    16441 ctaactacct agaagtttgt tacatcctgc tgaatggtga aaaaccgact caggaacagt
    16501 atgacgaatt taaaactacg gtgacccgtc ataccatgat ccacgagcag attacccgtc
    16561 tgttccacgc tttccgtcgc gactcacatc caatggcagt catgtgtggt attaccggcg
    16621 cgctggcggc gttctatcac gactcgctgg atgttaacaa tcctcgtcat cgtgaaattg
    16681 ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc gatgtgttac aagtattcca
    16741 ttggtcagcc atttgtttat ccgcgcaacg atctctccta tgccggtaac ttcctgaata
    16801 tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc gattctggaa cgtgctatgg
    16861 accgtattct gatcctgcac gctgaccatg aacagaacgc ctctacctcc accgtgcgta
    16921 ccgctggctc ttcgggggcg aacccgtttg cctgtatcgc agcaggtatt gcttcactgt
    16981 ggggacctgc gcacggtggt gctaacgaag cggcgctgaa aatgctggaa gaaattagct
    17041 ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga taaaaatgat tctttccgcc
    17101 tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc gcgcgccacc gtaatgcgtg
    17161 aaacctgcca tgaagttctg aaagagctgg gcaccaaaga tgacctgctg gaagtggcta
    17221 tggagctgga aaacatcgcg ctgaacgacc cgtactttat cgagaagaaa ctgtacccga
    17281 acgtcgattt ctactctggt atcatcctga aagcgatggg tattccgtct tccatgttca
    17341 ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc ccactggagc gaaatgcaca
    17401 gttacggtat gaagattgcc cgtccgcgtc agctgtatac aggatatgaa aaacgcgact
    17461 ttaaaagcga tatcaagcgt taatggttga ttgctaagtt gtaaatattt taacccgccg
    17521 ttcaaatggc gggttaattt ttatatgcct aaacacaaaa aattgtaaaa ataaaatcca
    17581 ctatcggaca ttcatatatt ctaaaaaaaa aacatcggta taaattataa acaaatagat
    17641 gatttttaat catctatctt cctctcagtt acatgaataa accaccaaat atagcgcacc
    17701 cctataattc ccaatacttt attcatcaaa catttaattc ataccatcaa ttaattactt
    17761 ttacatttaa taaaattaat aatattttta agaaaaacta aaaacattcg acaatcaaat
    17821 aagatattct tgcaccgata tacttttttc ttttctgtta aaggactcag ttatgtttaa
    17881 aggacaaaaa acattggccg cactggccgt atctctgctg ttcacagcac ctgtttatgc
    17941 ggctgatgaa ggatccggtg aaattcactt taaaggtgaa gttattgaag caccgtgtga
    18001 aatacatcag gatgatattg ataaagaggt tgaactcggt caggtgacca ccagccacat
    18061 taatcagtca catcacagcg atgccgttgc tgtcgacctg ctcttagtca actgtgatct
    18121 ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa gttgcagtca cctttgatag
    18181 ctcagcgaaa accaccggcg cagatccgat tctcaacaac accagcacgg gtgaagccac
    18241 cggcgtcggc gtacgtttaa tgaataaaga tcaaagcaat atcgttttag gcaccgctac
    18301 tccagatatc gacctggctc cgacctccag cgaacaaacg ctgaatttct ttgcctggat
    18361 ggaacaaatt gatcaggcta cacctgtaac gccaggcgca gttacggcga atgcaaccta
    18421 cgtgctggat tataaataag tattactttg gcggcgctat cgccgccttt cataaaatat
    18481 ttatcaggta tggacaccgt gaatatttat cgactctctt ttgtatcctg cctggtcgtg
    18541 gcgatgcctt gcgcattggc ggtcgaattc aatctgaatg ttctcgataa atcaatgcgc
    18601 gaccgcattg atatttcatt attaaaggaa aaaggagtca ttgctcccgg tgagtatttt
    18661 gttagcgttg cggtaaataa taaccaaatc agtaacgagc aaaagattaa ctggcacaaa
    18721 aatgacgata aaaccattcc gtgcatcaat gatttactgg tcgataaatt tggcttaaaa
    18781 cctgaagtcc gtcagtcgtt accattgata aatcagtgcg tcgattttag ctcccgacct
    18841 gaaatgctct tcaatttcga tcaagccaat cagcaactga atattagtat tccgcaagcc
    18901 tggctggcgt ggcactcaga aaactggacc ccaccctcca catggaaaga aggtgtcgcc
    18961 ggtgtcctga tggattacaa cttgtttgcc agcagctacc gcccacagga tggcagcagc
    19021 agcactaatc ttaacgccta tggaacagcc ggaatcaaca cgggggcatg gcgcttacgc
    19081 agtgattatc agttgaatca tactgatagc gatgataacc atgaacagtc aggcgaaata
    19141 tcacgtacct atctttttcg cccgttaccg caattaggtt caaaattaac cctcggcgaa
    19201 acggatttta gtcccaatat tttcgacggt ttttcttata ccggcgcggc actggcaagt
    19261 gacgatcgaa tgttgccatg ggaactacgc ggctacgccc cacaaattag cggtattgca
    19321 cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg tcatttacca gaaaaaaatc
    19381 ccaccaggcc catttatcat tgacgacctt aatcagtctg ttcagggcac actggatgtc
    19441 aaagtgacgg aagaagatgg tcgggtgaac aatttccagg tttcagcagc atcgacgccc
    19501 ttcctgactc gtcagggaca ggttcgctat aaactggccg cgggtcagcc acggccctcc
    19561 atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg ggggatgctg
    19621 tcaaacacct cgctgtacag cggcctgctg ctttctggtg atgactacca ttctgccgca
    19681 atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt cgtttgatgt cacgtgggcc
    19741 agtagccatt ttgatactca gcaggacgag cggggcttaa gctaccgttt taattacagc
    19801 aaacaagtgg atgccactaa cagcacgatt tccctcgccg cttatcgttt ctccgatcgt
    19861 cattttcaca gctacgccaa ctatctggat cacaaataca acgacagcga tgcgcaggac
    19921 gaaaaacaga cgatcagctt atctgtgggc caaccgatta ccccactaaa cctcaatctt
    19981 tacgccaacc tgctacatca aacctggtgg aatgcagacg cctccacgac cgccaacatc
    20041 acggcaggtt ttaatgttga tattggtgac tggagagata tctctatttc tacgtcattc
    20101 aatacaaccc attacgaaga taaagatcgc gacaaccaga tttacctgtc gatttcgctc
    20161 cccttcggta acggtggtcg ggttggctat gacatgcaaa acagtagcca cagcaccaca
    20221 caccgcatgt cgtggaacga tacgctggat gaacgtaata gctggggcat gtctgccgga
    20281 ctgcaatccg accgtcctga caatggagcc caggtgagcg gtaactatca gcacctgagt
    20341 tcagcgggtg agtgggatat ttctggtacc tatgccgcca atgattacag ttccgtcagc
    20401 agcagctgga gcggttcttt caccgcaacc caatatggtg cagcgtttca tcgccgcagc
    20461 tccaccaatg aaccacgcct gatggtcagc accgatggcg tggcagatat tccggttcag
    20521 ggcaatctcg actacaccaa ccattttggc attgcggtgg tgccgttgat ttccagttac
    20581 cagccttcca ccgtggcggt gaacatgaat gacttacccg acggcgtaac agttgcagaa
    20641 aacgttatca aagaaacgtg gattgaaggc gcgataggtt acaaatcact ggcttcccgt
    20701 tccggtaaag acggtaacgt catcattcgc aacgccagcg gtcagtttcc tcccctcgga
    20761 gcggatatcc gccaggatga cagcggcatt agcgtgggga tggttggcga ggaaggacat
    20821 gcctggttaa gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg tgatagccag
    20881 cattgctcgc tccatcttcc tgaacatatg gaagacaccg caaatcgcct gattttacct
    20941 tgtcattaat taaggaaatg aaaatgacat ttatgaaagg actacccctt ttgttgttgg
    21001 ttgccagtct gtgcagccac gctgcactac aacccgatcg cactcgtatt gtgtttaacg
    21061 ctaatgataa agctaccagt ctgcgggtgg ataatcgtag cgataaactc ccctacctcg
    21121 cttattcctg gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc
    21181 cgccgataca acgtctggaa ccgaaagcaa caacacaggt acggatagta aagcaagcct
    21241 cgaccacaaa attaccgggc gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc
    21301 ctcccgcccc ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt cagagtcgta
    21361 tcaaggtgtt ctggcgaccg gctgcattac gcaagaaagc aggagaaaag gtcgaactgc
    21421 agttacaggt cagccagcag ggcaaccaac tgacactgaa aaatcctacc gcgtattatc
    21481 tgaccattgc ctacctggga cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga
    21541 tggttgcgcc ttttagtacg gtcaacacaa acaccggaaa ttatagcggc agccagtttt
    21601 atctcggtta tatggatgat tacggcgcgt tacgcatgac gacgctcaac tgcagcggac
    21661 aatgccattt acaagccgtg gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt
    21721 catctgtctg ttatttctgt cgttgaagtc tgcgcttgcc ctgaactgct actttggttc
    21781 atccggtggc tcggtagaaa aatcagaagc gattcaacct tttgctgtac caggcaatgc
    21841 caaactcggc gataagatct gggaatctga cgatattaaa ataagatctg ggaatctgac
    21901 gatattaaaa ttcccgtcta ttgtgacaac aataccaacg gcaactttga aagcgaacat
    21961 gtctacgtat gggtaaaccc ctacccgggg gtgcaggatc gttattatca actcggagta
    22021 acttacaatg gcgttgatta tgacgccaac cagggaaaaa gccgcatcga cactaaccag
    22081 tgtatcgaca gtaagaacat tgacatttat acccctgatg ggatggcaga acaaaatttg
    22141 ctcgggtgat cccgctaata ttcacatgtc gcgaaccttt ctcgctcgca tgcggttata
    22201 cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt acgcttagcg actatatcgt
    22261 cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct actgcccaaa acctgaaata
    22321 tcatattact ggtctggaaa acattcgcgt gctggattgc agcgtcaatt tttccatttc
    22381 accggaaaca cagatgattg attttggtaa atttaatttg ctggatattc gtcgccacac
    22441 aatgtcgaaa acattcagta ttaagacgac aaaatcacaa aatgaccaat gcaccgacgg
    22501 atttaaagtc agttcctcct tttataccga agaaacgttg gttgaagaag ataaagcatt
    22561 gttgattggt aatggtctga agctacgttt gctggatgaa aacgcctcgc cttatacctt
    22621 caataaatat gctgaatacg ccgatttcac cagcgacatg ttggtctacg aaaaaaccta
    22681 tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag gctgggccct tcgatacagt
    22741 ggtgcttttt aaaattaact ataactgatt tgccatgatg ccctttgcag ccggaaaggg
    22801 catctctttc agaaaattgc tgattttccc cttttccttg attgctgtta ttttttccta
    22861 gcccggcttt ctttatgctg tatcgataca ccaaagagaa aataatgagg gagcgaagga
    22921 tgccagtttt gcagtgggga atgttatgtg tgctatcact tctcctttct attggtttcc
    22981 tcgcggtaca tctcccggcg gcgctattac ttgggccgat gatcgccggg atcatcttca
    23041 gtatgcgcgg aattactctg caacttcccc gctccgcttt tctcgccgcg caggccattc
    23101 ttggctgcat gattgcgcaa aacctcactg gttcgattct caccacgctg gccgccaact
    23161 ggccgatcgt gcttgctatt ttactggtga cgctgctttc cagcgccatc gtgggttggt
    23221 tattggtgcg ctatagctca ctgcctggaa ataccggtgc ctggggttct tctccaggcg
    23281 gcgcagcggc aatggtcgct atggcgcaag attacggcgc agatattcgc ctggtggcgt
    23341 ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc tgttctggtc acccgcatga
    23401 tgctgggtga taacgctgaa gcggttaacc agcaaatcgt ctggttcccg ccagtgagca
    23461 ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg tacggcggga tgcatgctgc
    23521 gtcttccttc cggcacgatg ctcatcccga tgctggcggg cgcagtgctc cagtctggtc
    23581 agctcatcac catcgaacta ccggaatggc tgctggcgat ggcgtatatg gcaattggct
    23641 ggcggattgg tcttggtttc gataaacaaa tcttactgcg ggcactacgc ccgctaccgc
    23701 aaatcctgct gtcgattttt gctctgctgg ctatttgtgc aggtatggcg tggggactga
    23761 cccggtttat gcatattgat tttatgactg cctacctcgc caccagtccc ggcgggcttg
    23821 ataccgtggc ggtcatcgcc gcagggagca atgccgatat ggcgcttatt atggcgatgc
    23881 aaaccctgcg cctgttcagt attttgctga cggggccagc cattgcgcgg tttatttcaa
    23941 cctatgcgcc gaagcggtcg gcctagtgct ggcagcctgg acaccagtaa aacgggcgcg
    24001 aggacaacgt ggttttctca atgatgccgc cacagcgttc gcacagttcg ccatcacgat
    24061 gaaaaacctt aaagcgaaac agcgccccat gatgcttatt ctcatccacc tgcccccgcg
    24121 tagcgtaaga aaaccgagga atctccagta acgcgtgtgc gagtgcatcc agttgcgccg
    24181 cattgagatc tttcgcttta tgatttccag tcaacccaac ctgccagagg atctccaccc
    24241 gcaaataatt gccaagccca gccagaaacg cctgatcgag cagtaatcct gcaaactgac
    24301 ggttacgaaa gcgcggcgac aataaccgtt cctttaccac ctccggcgtc agattcggat
    24361 ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt tccggcctca
    24421 acatctcaat atccgaggcg ctataaagca gaatggtttt gtcagccgtt tgcagtttta
    24481 cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc cagacgccgt
    24541 aaagctgatt atggctgtag agcgttaaac cgttgggaaa atgagttaac aacgccttac
    24601 cacgcgtttc cacatgggta acgtgttgac cgataagttg tgattgataa gtttttaact
    24661 gcgggaaggc aaaccagaca tcagttagtg gtttgccttt gatcgccgcc tccaggttat
    24721 ccgctgcacg gcggatctcc gggccttcag gcatggtgat atcctttttt cattctttat
    24781 taagcggggt tatgctgcga caacaatccc ctttttggca aatgtagctc gtaatcggcg
    24841 ggcgaacgcc agagcgtgct cgccgtcgcc atgcaggcag accgtttgcg ccgcgaccgt
    24901 tgcccattcg ccggtgatac ttttgactct gccgtgttgc accatctcca gcgtttgcgc
    24961 caacgcctgt tcttcgtttt caatcaacgc gcctggctga cttcgcggca ccagcgagcc
    25021 gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc gttgtcaggc catattgctt
    25081 gcctgcacga atcagctcgc ttcctgccag cccaacgaga accaatgccg gatcgcaagc
    25141 gtataccgct ctggcgatgg cgtctgccag ttgtgcttct ttcgccgcct ggttgtacaa
    25201 catgccgtgc ggtttgacat gacgcattac gccgccttgc gcacgggcaa tcgttgccag
    25261 cgcgccaatt tgatacagcg tctgggcgta aacggtttcc ggcggcagct gcatggcgct
    25321 gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg ccaatcgcga caccattttt
    25381 tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg cctgcatgaa atccacaagc
    25441 aatattggca gaggaaacca gcgttaatag ctctgcgtcg ctggcgcagc cttcgcccag
    25501 atcggcgttc aggtcaattt tcattgtgca gccgccacgc taattgttca aaataacgtt
    25561 gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca ctggacaaaa tgaatcggct
    25621 gaccgagcgg aatttgcgcc agatggtaca tatcagcctc aatgatacag gcaatacgtg
    25681 gataaccacc ggtggtctgt gcgtcgttca tcaacacaat cggctgcccg ttatgcggca
    25741 cctgcaccac gcccggtaac aaaccgtgag ataacagttc gcgatcggtg atgcgtttta
    25801 aaatttgccc ctgtaaacga tagcccattc ggttactttg cgagctaagc tgccagggcg
    25861 aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg atattccggc cccggcaagg
    25921 cgcgaatgcg gttgccccac agcagctgtt taacgccctg cgcttccata aaatcacgct
    25981 tcgatttgcc aatcgggagt cagtcaccat ccttcagtaa acggccttcc agcccgccaa
    26041 tccccacttt gagatcggtg ctgcatgacc ccattaccgg cggaacatca ataccacccg
    26101 cgaccgccag ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa cgctggcctg
    26161 ctttcatcgg caatcgccag ccggtccaga cggcgttatc atccaaccgc gcttcgcaac
    26221 ctgcacccgt cagggcaaac cacccatcag tttcgaactc aactgttaac tgaccgagcg
    26281 tgatctccag cgcgggggca ttggcgtcat tacccaccag caggttagca atgcgcagcg
    26341 caggcatatc cagtgcgcca cagtggctga tacccgactg gcaaaaaccg tgacgaccgc
    26401 catcctgcac agtggtgtac atgcccgcac gaataatctt cagcatattc cctccttctg
    26461 cggtacaaat cgcacgctgt ctcccggacg taataagatg ggttcgtcac gcgccggatc
    26521 aaacaggctg agtgaggtat gaccaatcaa ctgccagcca cccggcgttg ccagcggata
    26581 aacaccagtc tgcggcccgc cgatcccgac agaacctgcc ggaacgagca agcgcggttc
    26641 agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg agatacggga agcccggttg
    26701 aaaacctaaa aaccagacca cgtattccac ggaggagtgc aattcaacaa cctgtttttc
    26761 gctcaacccg caatacgccg cgaccaccgc caaatccggt ccgcctgcac caccgtaaac
    26821 caccggaatt tcaataaagc gagactcagg ttccagggcc tcgctctcct cccaccagcg
    26881 ttgcaaacgc tctatggcat ccagcgccag cgactcagga ttacgcaaaa tcaccgtgat
    26941 attgttcatg ccgggaatgg cttcaaccac attcggcata tccaccagac gctgcgccag
    27001 tcgccagatc cgtttctggc tagccagcgt caccggcggt tccagttcca gcactaccgc
    27061 cgtttcacct atcagataac aacgcgctcg ttgcactttc gatccctctg attattcgtt
    27121 atgcaggatt aggaatatca ataaaggtca catcaagatc ggtattttca ttcagccact
    27181 cgctcaatgc gcgaatacca ccgcgttcag tggcatggtg acctgcagca taaaaatgca
    27241 atccctgctc gcgggctgaa tgaatggtct gttcagaaac ttcgccagta ataaaagcat
    27301 ccacgccaaa acgcgcggcg ctatcgataa aactttgccc gccgcccgtg caccaggcga
    27361 cgcgctggac cacctcaggt ccggtatcgc cacaccataa cggcttacgt cccagacgcg
    27421 cttcaatcca ggaggctagt tccattcccg gcacaggcat ggtcagctct ccccacggca
    27481 ccaacggctc aatttcgccc atgaccgtga tccccagtaa cgccgccagc tgtgcgttat
    27541 tgcccagctc aggatgtgcg tcaagcggca aatgccagcc atacaggttg atatcattcg
    27601 ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat gaccggagac tctcctttcc
    27661 agaagtagcc atgatgcaca atgaccgcat ccgcgcccag acgcactgcc tcatcgagca
    27721 aagcctggct ggcggtgaca ccggtaacaa ttttttgcac cgtctctttg ccttccacct
    27781 gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg ttaatcagtt
    27841 gttccagttc ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta caccgttaac
    27901 atagcgttac atcgcctgtt cgtcgatagc caatcttttg acatattaag aaacaaaccc
    27961 gacgccaaaa gacgcaaatt gtttatatca tcacgcttca gctaatgtat atttatgcaa
    28021 tcagacagca tattgcataa ataatcagat ctgtatcaaa gttttaactt taaggaaaca
    28081 caagcacgcc gcgcaactat tacgtgacgt tgccgtcaat tgtggaatta ttatgaataa
    28141 acacgcatca caaccgcgcg ctatttacta tgtcgttgcg ctgcaaatct gggaatattt
    28201 tagcttttac ggcatgcgtg ccctgctgat tctctatctc accaatcaac taaaatacaa
    28261 cgatactcac gcctacgagt tatttagcgc ctactgttcg ctggtgtatg tcacgccaat
    28321 cctcggtggc tttttggcgg ataaagttct cggcaatcgc atggcggtga tgctgggggc
    28381 gttgttgatg gcgatcggtc atgtggtgct gggtgccagt gagatccatc cgtcattcct
    28441 ctatctgtcc ctggcgatta tcgtctgcgg ctatggcctg tttaaatcta acgtaagctg
    28501 tctgctcggc gagctgtatg agccaaccga tccgcgtcgt gatggcggtt tctcgctgat
    28561 gtatgcggcg ggtaacgtgg ggtctattat cgcacctatc gcctgtggtt acgcccagga
    28621 agagtacagc tgggcgatgg gctttggcct ggcggcggtt ggaatgatcg cgggtctggt
    28681 cattttctta tgtggcaatc gtcatttcac tcatacccgc ggcgttaaca aaaaagtact
    28741 gcgtgcgaca aactttctcc tgccgaactg gggatggctg ctggttctgc tggtggcaac
    28801 gcctgcactg attaccgtgc tgttctggaa agagtggtcg gtatacgcct taattgtcgc
    28861 gactattatt ggcctgggtg tactggcaaa aatttatcgc aaagcagaaa accagaaaca
    28921 gcggaaggag ctggggctga ttgtgacgct caccttcttc agtatgttgt tctgggcctt
    28981 cgcacaacag ggcggtagct cgattagcct ttatatcgac cgcttcgtta accgcgatat
    29041 gtttggttat accgttccga ccgcgatgtt ccagtcgatt aatgccttcg cagttatgct
    29101 gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc gtaccgtgcg
    29161 catctgggga aaatttgctc ttggccttgg cctgatgagc gccggattct gcattctgac
    29221 cttaagcgcc cgctggtccg caatgtatgg tcactcttct ctgccactga tggtgttagg
    29281 cctcgcggtg atgggctttg cggaactgtt tatcgacccg gttgccatgt cgcaaattac
    29341 gcgcattgaa attccgggcg tgaccggcgt attaaccggc atctacatgc tgctttctgg
    29401 cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag acatcgcagg cttcgtttga
    29461 tgcttccggg gcgatcaact actccatcaa tgcatatatt gaagtatttg atcaaattac
    29521 ctggggcgca ctggcgtgtg taggactggt actgatgatt tggctgtatc aggcgctgaa
    29581 attcagaaac cgcgcgctgg cgctggagtc ataataggcc agcaccgatg cctgatgcga
    29641 cgcttgacgc gtcttatcag gcctacgtat ttcctgcaat ttattgaatt tgcacaaatt
    29701 tgtaggctgg attaggcgtt cacgccgcat ccggcatctg ggctcgattg cctgacgcgt
    29761 ctgttatttc cccttccgcg ccgcctcata cgctgccaac gtttgtactc tcgcctcttt
    29821 gtgctcgact atcggttgcg gataatccag cttcacacct gctttctgcg cccacttcca
    29881 cggctcatgc accgctttcc ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa
    29941 ctcgccctca cggtcaaatt tctcgccctg agttatcgga ttgaaaatac gaaaatacgg
    30001 cgcagcatca gtacccgtag aagccgccca ctgccagcca ccgttattag ctgccaaatc
    30061 accatcaatc agctgcgaca tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc
    30121 tttcaccaga aaactggctg taatcatccg tagccggtta tgcatccagc cagtgctgtt
    30181 aagctgacgc atagcggcat caataatcgg gtatcccgtt ttgcctttct gccaggcctg
    30241 taaatgtgcg ggattgcttt gccactgcac acgatccgtc caggcaataa atggacgatg
    30301 tttacacaac gaggggtaat acgtcatcag atgacggtag aactcgcgcc agatcagctc
    30361 actaagccag acactaccgg ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg
    30421 atgcaaacac tggcgaggcg ataacccgcc cgttgccaag ctggcggaca aacggctggt
    30481 gccttccact gccggaaaat ctcgttgttg ctcatactct ccagcaccgt tctcgcaaaa
    30541 ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga
    30601 ctgacgagga taattcagcg taatggatgg cgcgggcttt atcgatccgc tactacgaac
    30661 ttttggtgca gcgacgcact ccggcatccc ttcccgcagc cgtttcagcc aggcattctt
    30721 aaaaggcgta aagactttgt acatctcgtg attgccggtc atcaccgccc ccggcggcag
    30781 gatcacgctg tcatcaaatc cttcacacac cacgttacgc agcgctcttt caacgtccac
    30841 atcccgcgct cgctcattca cctcatactg atagttataa aaaagatgag taacgctgtt
    30901 ttccgcgcac acctgtttaa ctatttcgac actggcgaca aagtcatcca cttcacggaa
    30961 caataaagga ataccttttt ccgccagcgc gatttgtagc ccattcagtt gagcattgat
    31021 aagttcagcc tgacgcggcg acatgttatg cgtctcccac ttgcgtggtg tagcgatata
    31081 cagcgccagc acgcgtgcag acgaattgcg gcaggcggca gccagtgcga gattatcgtg
    31141 cagacgtaaa tcctggcgaa accaggccag atgggtagtc ataaaactcc tgaatggcgc
    31201 aagttaatca cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga
    31261 taaagcgcca tatagtgttt gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg
    31321 cgataactgt cgataagcgc agccagctgc tggcgttctg tggaatcaat atgcgggcga
    31381 aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc gcgggttggc gggatgagaa
    31441 agcaaaacaa ttacgcgttg gcggtactgg ttgtaaaaat catccagatt ttgccactga
    31501 tgaatatcag ccacaaacgg gccaagtttg cggtactcgg gctgagaatg ggccagaaag
    31561 acgagtttat agcggctgtg ataatcgaga agcgccccgc gcgttaagcc atgttctttc
    31621 agtcgatgta actcatgaag ggcgaagata cggattaggg tgctgtcatc aaatcgttgt
    31681 agattcatta ctgctcctga acggtataca ggagtaagtg taggcgttgg taaatgatgt
    31741 ttgtgaagtt aatccgctag cagggcaaga ttacgaaagc gcgctccccg caagcaacta
    31801 atcaaggtag ttaatcatgg ctgaacacga tattacgcca gaagtcacta tttccaaaac
    31861 acagcgccgc tataaagtcg gctatgtcag agcccgccac caggaccgca aaaccggtat
    31921 agcccggtat tattcacaac atcccagtct gcaccttaag ggcgactggc tgaaagaggc
    31981 cggatttgat accggacggg gtgtcacggt gaagatttcg gaggggggcc tgaccatcat
    32041 cgctgacagt gacgaggtgc aggaactgcg ggatgggatg tttagtgtgc tgaacgaaag
    32101 ttaaatatta aaaaagctgg gaagatttaa aaccttccta gctttttatt ctgtcttatt
    32161 ataatggata ctcatcatct tccgatgaat ttataaattg ctccaagttt acatgaagag
    32221 tacttaacct agtttttaac tcattaagtg aagcgataat t
//
LOCUS       DAZOZX010000033        31874 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZOZX010000033 DAZOZX010000000
VERSION     DAZOZX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.033"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..860)
                     /gene="rarD"
                     /locus_tag="ACHUPT_001515"
     CDS             complement(<1..860)
                     /gene="rarD"
                     /locus_tag="ACHUPT_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0819136.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDA"
     gene            complement(912..1379)
                     /gene="yigI"
                     /locus_tag="ACHUPT_001516"
     CDS             complement(912..1379)
                     /gene="yigI"
                     /locus_tag="ACHUPT_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0819137.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1544..2413
                     /gene="pldA"
                     /locus_tag="ACHUPT_001517"
     CDS             1544..2413
                     /gene="pldA"
                     /locus_tag="ACHUPT_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0819138.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2546..4375
                     /gene="recQ"
                     /locus_tag="ACHUPT_001518"
     CDS             2546..4375
                     /gene="recQ"
                     /locus_tag="ACHUPT_001518"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0819139.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4439..5059
                     /gene="rhtC"
                     /locus_tag="ACHUPT_001519"
     CDS             4439..5059
                     /gene="rhtC"
                     /locus_tag="ACHUPT_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0819140.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5121..5741)
                     /gene="rhtB"
                     /locus_tag="ACHUPT_001520"
     CDS             complement(5121..5741)
                     /gene="rhtB"
                     /locus_tag="ACHUPT_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0819141.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5852..6874
                     /gene="pldB"
                     /locus_tag="ACHUPT_001521"
     CDS             5852..6874
                     /gene="pldB"
                     /locus_tag="ACHUPT_001521"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0819142.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6882..7682
                     /gene="yigL"
                     /locus_tag="ACHUPT_001522"
     CDS             6882..7682
                     /gene="yigL"
                     /locus_tag="ACHUPT_001522"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0819143.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7758..8657
                     /gene="bioP"
                     /locus_tag="ACHUPT_001523"
     CDS             7758..8657
                     /gene="bioP"
                     /locus_tag="ACHUPT_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0819144.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8545..9498)
                     /gene="metR"
                     /locus_tag="ACHUPT_001524"
     CDS             complement(8545..9498)
                     /gene="metR"
                     /locus_tag="ACHUPT_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0819145.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9734..11995
                     /gene="metE"
                     /locus_tag="ACHUPT_001525"
     CDS             9734..11995
                     /gene="metE"
                     /locus_tag="ACHUPT_001525"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0819146.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12034..12849)
                     /locus_tag="ACHUPT_001526"
     CDS             complement(12034..12849)
                     /locus_tag="ACHUPT_001526"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0819147.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13111..13872
                     /gene="udp"
                     /locus_tag="ACHUPT_001527"
     CDS             13111..13872
                     /gene="udp"
                     /locus_tag="ACHUPT_001527"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0819148.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14013..15440
                     /gene="rmuC"
                     /locus_tag="ACHUPT_001528"
     CDS             14013..15440
                     /gene="rmuC"
                     /locus_tag="ACHUPT_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0819149.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15535..16290
                     /gene="ubiE"
                     /locus_tag="ACHUPT_001529"
     CDS             15535..16290
                     /gene="ubiE"
                     /locus_tag="ACHUPT_001529"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0819150.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16304..16909
                     /gene="ubiJ"
                     /locus_tag="ACHUPT_001530"
     CDS             16304..16909
                     /gene="ubiJ"
                     /locus_tag="ACHUPT_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0819151.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16906..18546
                     /gene="ubiB"
                     /locus_tag="ACHUPT_001531"
     CDS             16906..18546
                     /gene="ubiB"
                     /locus_tag="ACHUPT_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0819152.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18625..18894
                     /gene="tatA"
                     /locus_tag="ACHUPT_001532"
     CDS             18625..18894
                     /gene="tatA"
                     /locus_tag="ACHUPT_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0819153.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18898..19413
                     /gene="tatB"
                     /locus_tag="ACHUPT_001533"
     CDS             18898..19413
                     /gene="tatB"
                     /locus_tag="ACHUPT_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0819154.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19416..20192
                     /gene="tatC"
                     /locus_tag="ACHUPT_001534"
     CDS             19416..20192
                     /gene="tatC"
                     /locus_tag="ACHUPT_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0819155.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20234..21016
                     /gene="tatD"
                     /locus_tag="ACHUPT_001535"
     CDS             20234..21016
                     /gene="tatD"
                     /locus_tag="ACHUPT_001535"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0819156.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21013..21501)
                     /gene="rfaH"
                     /locus_tag="ACHUPT_001536"
     CDS             complement(21013..21501)
                     /gene="rfaH"
                     /locus_tag="ACHUPT_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0819157.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21668..23161
                     /gene="ubiD"
                     /locus_tag="ACHUPT_001537"
     CDS             21668..23161
                     /gene="ubiD"
                     /locus_tag="ACHUPT_001537"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0819158.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23207..23908
                     /gene="fre"
                     /locus_tag="ACHUPT_001538"
     CDS             23207..23908
                     /gene="fre"
                     /locus_tag="ACHUPT_001538"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0819159.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24100..25263)
                     /gene="fadA"
                     /locus_tag="ACHUPT_001539"
     CDS             complement(24100..25263)
                     /gene="fadA"
                     /locus_tag="ACHUPT_001539"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0819160.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25273..27462)
                     /gene="fadB"
                     /locus_tag="ACHUPT_001540"
     CDS             complement(25273..27462)
                     /gene="fadB"
                     /locus_tag="ACHUPT_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0819161.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27652..28983
                     /gene="pepQ"
                     /locus_tag="ACHUPT_001541"
     CDS             27652..28983
                     /gene="pepQ"
                     /locus_tag="ACHUPT_001541"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0819162.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28983..29597
                     /locus_tag="ACHUPT_001542"
     CDS             28983..29597
                     /locus_tag="ACHUPT_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0819163.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29636..31087
                     /gene="trkH"
                     /locus_tag="ACHUPT_001543"
     CDS             29636..31087
                     /gene="trkH"
                     /locus_tag="ACHUPT_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0819164.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31099..31644
                     /gene="hemG"
                     /locus_tag="ACHUPT_001544"
     CDS             31099..31644
                     /gene="hemG"
                     /locus_tag="ACHUPT_001544"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0819165.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7405 a         8119 c         8728 g         7622 t
ORIGIN      
        1 gcgtccatca caaaaattgc cagcgccacc caaataaagg tgaaagtcac catcttatcg
       61 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
      121 tactgaaaaa agcctaacgt tgagagacgc aggcgcgtgg cagcggcagt aaaacacaac
      181 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
      241 tgccccatat ggctggttga actgtcggca atagcaaaca ggtaaatcgc cgccacgggc
      301 agcagccaca tggtttcaat taacatgccg gtttgtgctt ctaccgcaat cttcttgcgc
      361 accaggccgt agaaggcaaa actaaatgcc agccccagcg cgataatagg tagcgaacca
      421 aaagtccaca gctggaccaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
      481 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
      541 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
      601 cagttgccac caatcagtac ggcagagact gccagcataa aaattttctg tggcgtctga
      661 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
      721 aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcagcttg
      781 aagtatgctg gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
      841 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
      901 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
      961 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     1021 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     1081 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     1141 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gactccgccg
     1201 tgcaaaatgc tttgcgccca gttacccacc atcatcggct gatttttaaa ggccagctgt
     1261 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     1321 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     1381 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     1441 ctactttagg aagggataac taacggcaat ggagttaagt atgtaaaata gcccgcagaa
     1501 aaacattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     1561 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     1621 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     1681 tgacaatccg ttcacgctct atccttatga caccaactac ctcatctaca cccaaaccag
     1741 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     1801 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccaaactc
     1861 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     1921 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     1981 ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     2041 gcgttcagac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     2101 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     2161 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     2221 ggtgctcagt gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg
     2281 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     2341 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtagggg ttatgctaaa
     2401 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaatccagca tccaggatta
     2461 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     2521 tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     2581 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     2641 aattatcgac actgtgcttt ccggtcgcga ttgcctggtt gttatgccca ctggtggcgg
     2701 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     2761 accgctgatt tcgttgatga aagatcaggt ggatcaacta caggccaacg gcgtggcggc
     2821 ggcgtgcctt aactcgacgc aaacccgcga acagcaactt gaagtgatga caggctgccg
     2881 caccgggcaa attcgcttac tgtatatcgc cccggaacgc ctgatgctgg ataactttct
     2941 tgagcatctg gcgcactgga atccggtgtt attagccgtc gatgaagcgc actgtatctc
     3001 ccaatggggt cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt
     3061 cccgacgctg ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat
     3121 cgtgcgcctg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     3181 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     3241 ggaacagcgc ggtaagtctg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     3301 cgctgcgcgc ctgcaaagca ggggtattag cgcggcggcc tatcatgccg ggctggaaaa
     3361 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gatctgcaaa ttgtggtggc
     3421 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     3481 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     3541 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     3601 ggaagagaag ccgcaggggc agttgcagga tatcgagcgc cacaaactca atgcgatggg
     3661 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     3721 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     3781 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     3841 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     3901 tgacaaactg aaagtctatg gtatgggccg tgataaaagc catgaacatt gggtgagcgt
     3961 gatccgccag ctgattcatc tcggcctggt gacgcaaaat attgcccagc attctgccct
     4021 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     4081 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     4141 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     4201 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     4261 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     4321 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     4381 agcataaaaa agtgccagta tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     4441 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     4501 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     4561 cgtgctgggc attacctgcg gcgtaatggt ttgggctggg attgcgttgc ttggcctgca
     4621 tttgattatc gaaaaaatgg cctggctgca tacgctgatt atggtaggcg gtggcctgta
     4681 tctctgctgg atgggttacc agatgctacg cggtgcactg aaaaaagagg ctgtatctgc
     4741 gcctgcacca caggttgagc tggcgaaaag cgggcgcagt ttcctgaaag gcttactgac
     4801 caatctcgcc aatccgaaag cgattatcta ttttggttcg gtattctcat tgtttgtcgg
     4861 cgataacgtt ggcactaccg agcgctgggg cattttcgcg ttgattattg tcgaaacact
     4921 ggcgtggttt accgtcgttg ccagtctgtt tgccctgccg caaatgcgcc gtggttatca
     4981 acgtctggcg aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca
     5041 tttgattatt tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac
     5101 gccgcatccg acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc
     5161 ataaacaacg agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat
     5221 agagcaatcc tttgagcaag ggtggcgtaa ccgatcatca caataatatc gaccacaata
     5281 gtggtgacgc cgagcacgat atactgcatc agttgcggct gttgcggcat gatgaattgc
     5341 ggaaatagcg ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg
     5401 cgctggaaca aatgtcgacg cgattgagta gaggccagcg atttaagatc aattgcgcca
     5461 gcggcgcgcc actgctggat tcccagccaa atcaagtaag ccgcgcctgc ccactttaac
     5521 acttcaaacg caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca
     5581 atatgaatcg ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg
     5641 tggttgagcg aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg
     5701 atcgatgtca gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc
     5761 tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt caggcacgct taaaaaaacg
     5821 attttacgtg gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac
     5881 aagagaaaac gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg
     5941 tgatgaagca gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc
     6001 acagcaccat gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata
     6061 tgcggaactg gcctacgacc tgttccattt ggggtttgat gtcttaatca tcgaccatcg
     6121 cgggcaggga cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt
     6181 taatgattat gttgatgatc tggcggcatt ctggcagcag gaggttcagc ccggtccgtg
     6241 gcgtaaacgc tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca
     6301 acgccatcca ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat
     6361 tcgtatgccg tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt
     6421 ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt
     6481 actgacccac agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac
     6541 gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca
     6601 ggtgttagcc ggtgcgggtg atgacgccac gcaaacgctt ctcttacaag ctgaagagga
     6661 acgcgtggtg gataaccgca tgcacgaccg tttttgtgaa ctccgcaccg ccgcgggcca
     6721 tcctgtcgaa ggaggacggc cgttggtaat aaaaggtgct taccatgaga tcctttttga
     6781 aaaggacgca atgcgctcag tcgcgctcca cgccatcgtt gattttttca acaggcataa
     6841 ctcacccagc ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt
     6901 ctgatttaga tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa
     6961 ctctgaagct gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg
     7021 ttgatgtggg gcaaattcgc gataatctgg agattaagtc ttacatgatt acctccaatg
     7081 gtgcgcgcgt tcacgatctg gatggcaatc tgatttttgc tcataacctg gatcgcgaca
     7141 ttgccagcga tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc
     7201 gcgacgacga atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag
     7261 cggtgttcaa atatgcgctg tatgagccgg gattactgga gccggaaggc atcagcaaag
     7321 tgtttttcac ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc
     7381 gctggggcga tcgcgtcaac gtcagtttct ctaccttaac ctgtctggaa gtgatggcgg
     7441 gcggcgtgtc gaaaggccat gcgctggaag cagtggcgaa gaaactgggc tacagcctga
     7501 aggattgtat tgcgtttggt gacgggatga acgacgccga aatgctgtcg atggcgggga
     7561 aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga tctgcatccg gagctggaag
     7621 ttattggtac taatgccgac gacgcggtgc cgcattatct gcgtaaactc tatttatcgt
     7681 aatcgttctt tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa
     7741 tcaaagagtt ttccattgtg gcgttactta tcatcaccac gattctgtgg gccttctcct
     7801 ttagctttta tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc
     7861 gcgttggctt ggcggcactc gtttttctgc cgtttctgcg tacccgtagc aatagcctga
     7921 aaacggtcgg cctgtatatg ctggtgggcg cgatgcagct cggcgtgatg tatatgctga
     7981 gtttccgcgc ttatctctac ctgacggtct ccgaattgtt gctgttcacc gtactgacgc
     8041 cgctctacat cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggctatg
     8101 cttttagcgc cttgctggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg
     8161 accatttctg gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca
     8221 tggtgggtta caaacgcctg atggaaactc gcccgatgcc gcagtataac gcctttgcgt
     8281 ggttctatct tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc
     8341 agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt
     8401 ccgggattgg ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg
     8461 gcattatgaa taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc
     8521 aacagccgca ctggccaacg tttattacag gcgcgctggt gatcctggcc tcactgtggg
     8581 tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt
     8641 gcgcgctgag cgaataaacg cttccgtaac tggctgacgc tgctcgccat cgcgcacggc
     8701 ggcgtacagt cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg
     8761 ctcaaaactc tctactaccc aatgcggtag cgcggcaata cccatccgcg cggcaaccat
     8821 ttgaatcaac aataaggtgt tatcgacgct tttcagtgac gggctgacgc ctgccggctg
     8881 aagaaaatgc cgccagacat ccagtcgact acgctgcacc ggataaatta atagcgtctc
     8941 gctggcgaga tcttccggtg taatacgcgt ttttgccgcc agtggatggt caggtgctaa
     9001 caccaggcgt acttcataat cgaacatcgg cgaataatgc aggccactgc gcggcaggat
     9061 atcggaggtc atcaccagat ccagctcgcc ctgttgcaac gcgggctgcg ggtcaaacgt
     9121 cacgcccgat ttaaaatcca tctctacctg cggccagttc ttatggaaat ttcctaaagc
     9181 gggtgtcagc cactgaatac agctatggca ctcaatggca atgcgcagac gcgtctgctg
     9241 cggttcattg caggcttgca gtgcctggct gatttgcggc agcacctggt ttgccagttg
     9301 caacaggatt tctccctgcg gtgtaaagcg tagcggctgg ctcttacgca caaatagccg
     9361 gaagccaagg cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtctgatg
     9421 caacgttgcc gcagcggctg cgagcgagcc gcagttccgc aacgcttgta gcgttttcag
     9481 gtgttttact tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa
     9541 tatacagtaa ccgccaatta tggatgtgta aacatctgga cggctaaaat cattcgtctt
     9601 ttaaatttat ggtgcgttgg ctgcgtttct ccaccccggt cacttacttc agtaagctcc
     9661 cggcgatgaa taaacttgcc gccttcccta aattcaaaat ccataggatt tacatataat
     9721 tagaggaaga aaaatgacaa tattgaatca caccctcggt ttccctcgcg ttggcctgcg
     9781 tcgcgagctg aaaaaagcgc aagaaagtta ttgggcgggg aactccacgc gtgaagaact
     9841 gctgacagta gggcgtgaac tgcgtgctcg tcactgggat caacaaaagc aagcgggtat
     9901 cgacctgctg ccggtgggcg attttgcctg gtacgatcat gtactgacca ccagtttgct
     9961 gctgggtaat gttccgccac gtcatcagaa caaagacggt tcggtagata tcgacaccct
    10021 gttccgtatt ggtcgtggac gtgcgccgac tggcgaacct gcggcggcag cggaaatgac
    10081 caaatggttt aacaccaact atcactacat ggtgccggag ttcgttaaag gccaacagtt
    10141 caaactgact tggacgcagc tgctggagga agtggacgag gcgctggcgc tgagccacaa
    10201 cgttaagcct gtgctgctgg ggccggttac ttacctgtgg ctggggaaag tgaaaggtga
    10261 acagtttgac cgccttagcc tgctgaacga cattctgccg gtttatcagc aagtgctggc
    10321 agaactggcg aaacgcggca tcgagtgggt acagattgat gaacccgcgc tggtactgga
    10381 actaccacag gcgtggctgg acgcatacaa acccgcttac gacgcgctcc agggacaggt
    10441 gaaactgctg ctgaccacct attttgaagg cgtaacgcca aatctcgaca cgattactgc
    10501 gctgcctgtt caggggctgc atgtggacct tgtacatggt aaagataacg ttgttgaact
    10561 gcacaagcgc ctgccttctg actggctgct gtctgcgggt ctgatcaatg gtcgtaacgt
    10621 ctggcgcgcc gatcttactg agaaatatgc gcaaattaag gacattgtcg gcaaacgtga
    10681 tttgtgggtg gcatcttcct gctcgttgct gcacagcccc atcgacctga gcgtggaaac
    10741 gcgtcttgat gcagaagtga aaagctggtt tgccttcgcc ctacaaaaat gccatgaact
    10801 ggcactgctg cgcgatgcgc tgaacagtgg tgacacggca gctctggcag agtggagcgc
    10861 cccaattcag gcgcgtcgtc actctactcg tgtacataat ccggcggtag aaaagcgtct
    10921 ggcggcgatc accgcccagg acagccagcg tgcgaatgtc tatgaagtgc gtgctgaagc
    10981 ccagcgtgcg cgttttaaac tgccagcgtg gccgaccacc acgattggtt ccttcccgca
    11041 aaccacggaa attcgtaccc tgcgtctgga tttcaaaaag ggcaatctcg acgccaacaa
    11101 ctaccgcacg ggcattgcgg aacatatcaa gcaggccatt gttgagcagg aacgtttggg
    11161 actggatgtg ctggtacatg gcgaggccga gcgtaatgac atggtggaat actttggcga
    11221 gcacctcgac ggatttgtct ttacgcaaaa cggttgggta cagagctacg gttcccgctg
    11281 cgtgaagcca ccgattgtca ttggtgacat tagccgcccg gcaccgatta ccgtggagtg
    11341 ggcgaagtat gcgcaatcgc tgaccgacaa accggtgaaa gggatgctga cggggccggt
    11401 gaccatactc tgctggtcgt tcccgcgtga agatgtcagc cgtgaaacca tcgccaaaca
    11461 gattgcgctg gcgctgcgtg atgaagtggc cgatctggaa gccgctggaa ttggcatcat
    11521 ccagattgac gaaccggcgc tgcgcgaagg tttaccgctg cgtcgtagcg actgggatgc
    11581 gtatctccag tggggcgtag aggccttccg tatcaacgcc gccgtggcga aagatgacac
    11641 acaaatccac actcacatgt gttattgcga gttcaacgac atcatggatt cgattgcggc
    11701 gctggacgca gacgtcatca ccatcgaaac ctcgcgttcc gacatggagt tgctggagtc
    11761 gtttgaagag tttgattatc caaatgaaat cggtcctggc gtctatgaca ttcactcgcc
    11821 aaacgtaccg agcgtggaat ggattgaagc cttgctgaag aaagcggcaa aacgcattcc
    11881 ggcagagcgc ctgtgggtca acccggactg tggcctgaaa acgcgcggct ggccagaaac
    11941 ccgcgcggca ctggcgaaca tggtgcaggc ggcgcagaat ttgcgtcggg ggtaaatcca
    12001 aaccgggtgg taataccacc cggtcttttc tcattacagc gacttcttcc caccatactg
    12061 cttaaaccat tccagcatac gctgccagcc atctttcgca gattcggcat gatagctcgg
    12121 gcgataatca gcgttgaatg catgcccggc atccgggtac acgataatct ctgctttcgc
    12181 attagcagcc cgcagcgcct ggcgcatcgt ttcaacgctc tcctgcggaa tgctgttatc
    12241 ctgaccacca tataagccga gaaccggcgc gttaagatcg gttgcgatat caacaggttg
    12301 tttcggtgaa ttcagcgact tgtcgcccgt cagtttgccg taccacgcca ctgcggcttt
    12361 tagctgtgga ttatgcgcgg catacagcca ggtgatacgt ccaccccagc agaatccggt
    12421 gatcattaaa cgatgaacat cgccgccgtt gcgggaagcc caactggcga catgatcgag
    12481 atcggccagc acctgcgagt caggcacttt tgctaccaga ccgctaagca acgtggggat
    12541 atcggcaaaa tcattcggat cgccttcgcg gaagtaaagt tcaggtgcga tagccagata
    12601 cccctccagc gccagacggc gacaaatgtc gcggatatgt tcatgcacgc caaaaatttc
    12661 ctgcactaca atgaccactg gcagtgggcc atcgctttgc tttggtctgg catggtaagc
    12721 aggcatgtta tccccttgtg aagggataga ggtgaagccc gccacaatcg cgtcgtccgg
    12781 ggtctgaacg atggtcgaag cgagaggcga tgcagcaggt gcaaatccag attgttgtgt
    12841 tgttgccatg gtattctccg tacccttata aaaatgttgc gcaatgttaa ctatagtcag
    12901 catgcaacaa atcacattgc ctgaatcggc tcatctttta tgcagtcctg cagaatgaag
    12961 agtgatttat gtgatatgaa tcacttttgg tgggtaaatt tatgcaacgc atttgcgtca
    13021 tggtgatgag tatcacgaaa aaatgttaaa cccttcggta aagtgtcttt ttgcttcttc
    13081 tgactaaacc gattcacaga ggagttgtat atgtccaagt ctgatgtttt tcatctcggc
    13141 ctcactaaaa acgatttaca aggggctacg cttgccatcg tccctggcga cccggatcgt
    13201 gtggaaaaga tcgccgcgct gatggataag ccggttaagc tggcatctca ccgcgaattc
    13261 actacctggc gtgcagagct ggatggtaaa cctgttatcg tctgctctac cggtatcggc
    13321 ggcccgtcta cctctattgc tgttgaagag ctggcacagc tgggcattcg caccttcctg
    13381 cgtatcggta caacgggcgc tattcagccg catattaatg tgggtgatgt cctggttacc
    13441 acggcgtctg tccgtctgga tggcgcgagc ctgcacttcg caccgctgga attcccggct
    13501 gtcgctgatt tcgaatgtac gactgcgctg gttgaagctg cgaaatctat tggcgcgaca
    13561 actcacgttg gcgtgacagt ttcttctgat accttctacc caggtcagga acgttacgat
    13621 acttactctg gtcgcgtagt tcgtcacttt aaaggttcta tggaagagtg gcaggcgatg
    13681 ggcgtaatga actatgaaat ggaatctgca actctgctga ccatgtgtgc aagtcagggc
    13741 ctgcgtgccg gtatggtagc gggtgttatc gttaaccgca cccagcaaga gatcccgaat
    13801 gctgagacga tgaaacaaac cgaaagccat gcggtgaaaa tcgtggtgga agcggcgcgt
    13861 cgtctgctgt aattctcttc tcctgtctga aggccgacgc gttcggcctt ttgtattttt
    13921 gcgtagcgcc tcgcaggaaa tgcctttcca actggacgtt tgtacagcac aattctattt
    13981 tgtgcgggta agttgttgcg tcaggaggcg ttgtggattt ctcaatcatg gtttacgcag
    14041 ttattgcgtt ggtgggtgtg gcaattggct ggctgtttgc cagttatcaa catgcgcagc
    14101 aaaaagccga gcaattagct gaacgtgaag agatggtcgc ggagttaagc gcggcaaaac
    14161 aacaaattac ccaaagcgag cactggcgtg cagagtgtga attgctcaat aacgaagtgc
    14221 gcagcctgca aagtattaac acctctctgg aggccgatct gcgtgaagta accacgcgga
    14281 tggaagccgc acagcaacat gctgacgata aaattcgcca gatgattaac agcgagcagc
    14341 gcctcagtga gcagtttgaa aacctcgcca accgtatttt tgagcacagc aatcgccggg
    14401 ttgatgagca aaaccgtcag agtctgaaca gcctgttgtc gccgctacgt gaacaactgg
    14461 acggtttccg ccgtcaggtt caggacagct tcggtaaaga agcacaagaa cgccataccc
    14521 tgacccacga aattcgcaat ctccagcaac tcaacgcgca aatggcccag gaagcgatca
    14581 acctgacgcg cgcgctgaaa ggcgacaata aaacccaggg caactggggc gaggtagtat
    14641 tgacgcgggt gctggaggct tccggtctgc gtgaagggta tgaatatgaa acccaggtca
    14701 gcatcgaaaa tgacgcccgc tcgcggatgc agccggatgt catcgtgcgg ctgccgcagg
    14761 gaaaagatgt ggtgatcgac gccaaaatga cgctggtcgc ctatgaacgc tattttaacg
    14821 ccgaagacga ctacacccgc gaaagcgcgc tacaggaaca tatcgcgtcg gtgcgtaacc
    14881 atatccgttt gctgggacgc aaagattatc aacagctgcc ggggctgcga actctggatt
    14941 acgtgctgat gtttattccc gttgaacccg cttttttact ggcgcttgac cgccagccgg
    15001 agctgatcac cgaagcgttg aaaaacaata tcatgctggt tagcccgact acgctgctgg
    15061 tggcgttgcg tactattgcc aacctgtggc gttatgagca tcaaagccgt aacgcccagc
    15121 aaatcgccga tcgtgccagc aagctgtacg acaagatgcg tttgttcatc gatgacatgt
    15181 ccgcgattgg tcaaagtctc gacaaagcgc aggataacta ccgccaggca atgaaaaaac
    15241 tctcttcagg tcgcggaaat gtgctggcgc aggcagaagc gtttcgcggt ttaggagtag
    15301 aaattaaacg cgagattaat ccggatttgg ctgaacaggc ggtgagccag gatgaagagt
    15361 atcgacttcg gtcggttccg gagcagccga atgatgaagc ttatcaacgc gatgatgaat
    15421 ataatcagca gtcgcgctag cccattaggg gtagttaagc cgggtagaaa tctagggcat
    15481 cgacgcccaa tctgttacac ttctggaaca attttttgat gagcaggcat tgagatggtg
    15541 gataagtcac aagaaacgac gcactttggt tttcagaccg tcgcgaagga acaaaaagcg
    15601 gatatggtcg cccacgtttt ccattccgtg gcatcaaaat acgatgtcat gaatgatttg
    15661 atgtcatttg gtattcatcg tttgtggaag cgattcacga ttgattgcag cggcgtacgc
    15721 cgtgggcaga ccgtgctgga tctggctggt ggcaccggcg acctgacagc gaaattctcc
    15781 cgcctggtcg gagaaactgg caaagtggtc cttgctgata tcaatgaatc catgctcaaa
    15841 atgggacgcg agaagctgcg taatatcggt gtgattggca acgttgagta tgttcaggcg
    15901 aacgctgagg cgctgccgtt cccggataac acctttgatt gcatcaccat ttcgtttggt
    15961 ctgcgtaacg tcaccgacaa agataaagca ctgcgttcaa tgtatcgcgt gctgaaaccc
    16021 ggcggccgcc tgctggtgct tgagttctcg aagccaatta tcgagccgct gagcaaagcc
    16081 tatgatgcat actccttcca tgtgctgccg cgtattggct cactggtcgc gaacgacgcc
    16141 gacagctacc gttatctggc agaatccatc cgtatgcatc ccgatcagga taccctgaaa
    16201 gccatgatgc aggatgccgg attcgaaagt gtcgactact acaatctgac ggcaggggtt
    16261 gtggcgctgc atcgtggtta taagttctga caggagaccg gaaatgcctt ttaaaccttt
    16321 agtgacggca ggaattgaaa gtctgctcaa caccttcctg tatcgctcac ccgcgctgaa
    16381 aacagcccgc tcgcgtctgc tgggtaaagt attgcgcgtg gaggtaaaag gcttttcgac
    16441 gtcattgatt ctggtgttca gcgaacgcca ggttgatgta ctgggcgaat gggcaggcga
    16501 tgctgactgc accgttatcg cctacgccag tgtgttgccg aaacttcgcg atcgccagca
    16561 gcttaccgca ctgattcgca gtggtgagct ggaagtgcag ggcgatattc aggtggtgca
    16621 aaacttcgtt gcgctggcag atctggcaga gttcgaccct gcggaactgc tggcccctta
    16681 taccggtgat atcgccgctg aaggaatcag caaagccatg cgcggaggcg caaagttcct
    16741 gcatcacggc attaagcgcc agcaacgtta tgtggcggaa gccattactg aagagtggcg
    16801 tatggcaccc ggtccgcttg aagtggcctg gtttgcggaa gagacggctg ccgtcgagcg
    16861 tgctgttgat gccctgacca aacggctgga aaaactggag gctaaatgac gccaggtgaa
    16921 gtacggcgcc tatatttcat cattcgcact tttttaagct acggacttga tgaactgatc
    16981 cccaaaatgc gtatcaccct gccgctacgg ctatggcgat actcattatt ctggatgcca
    17041 aatcggcata aagacaaact tttaggtgag cgactacgac tggccctgca agaactgggg
    17101 ccggtttgga tcaagttcgg gcaaatgtta tcaacccgcc gcgatctttt tccaccgcat
    17161 attgccgatc agctggcgtt attgcaggac aaagttgctc cgtttgatgg caagctggcg
    17221 aagcagcaga ttgaagctgc aatgggcggc ttgccggtag aagcgtggtt tgacgatttt
    17281 gaaatcaagc cgctggcttc tgcttctatc gcccaggttc ataccgcgcg attgaaatcg
    17341 aatggtaaag aggtggtgat taaagtcatc cgcccggata ttttgccggt cattaaagcg
    17401 gatctgaaac ttatctaccg tctggctcgc tgggtgccgc gtttgctgcc ggatggtcgc
    17461 cgtctgcgcc caaccgaagt ggtgcgcgag tacgaaaaga ccttgattga tgaactgaat
    17521 ttgctgcggg aatctgccaa cgccattcag cttcggcgca attttgaaga cagcccgatg
    17581 ctctacatcc cggaagttta ccctgactat tgtagtgaag ggatgatggt gatggagcgc
    17641 atttacggca ttccggtgtc tgatgttgcg gcgctggaga aaaacggcac taacatgaaa
    17701 ttgctggcgg aacgcggcgt gcaggtgttc ttcactcagg tctttcgcga cagctttttc
    17761 catgccgata tgcaccctgg caacatcttc gtaagctatg aacacccgga aaacccgaaa
    17821 tatatcggca ttgattgcgg gattgttggc tcgctaaaca aagaagataa acgctatctg
    17881 gcagaaaact ttatcgcctt ctttaatcgc gactatcgca aagtggcaga gctacacgtc
    17941 gattctggct gggtgccacc agataccaac gttgaagagt tcgaatttgc cattcgtacg
    18001 gtctgtgaac ctatctttga gaaaccgctg gccgaaattt cgtttggaca tgtactgtta
    18061 aatctgttta atacggcgcg tcgcttcaat atggaagtgc agccgcaact ggtgttactc
    18121 cagaaaaccc tgctctatgt cgaaggggta ggacgccagc tttatccgca gctcgattta
    18181 tggaaaacag cgaagccttt cctggagtcg tggattaaag atcaggtcgg tattcctgct
    18241 ctggtgagag catttaaaga aaaagcgccg ttctgggtcg aaaaaatgcc agaactgcct
    18301 gaactggttt acgacagttt gcgccagggc aagtatttac agcacagtgt tgataagatt
    18361 gcccgcgagc ttcagtcaaa tcatgtacgt cagggacaat cgcgttattt tctcggaatt
    18421 ggcgctacgt tagtattaag tggcacattc ttgttggtca gccgacctga gtgggggctg
    18481 atgcccggct ggttaatggc aggtggtctg atcgcctggt ttgtcggttg gcgcaaaaca
    18541 cgctgatttt ttcatcgctc aaggcgggcc gtgtaacgta taatgcggct ttgtttaatc
    18601 atcatctacc acagaggaac atgtatgggt ggtatcagta tttggcagtt attgattatt
    18661 gccgtcatcg ttgtactgct ttttggcacc aaaaagctcg gctccatcgg ttccgatctt
    18721 ggtgcgtcga tcaaaggctt taaaaaagca atgagcgatg atgaaccaaa gcaggataaa
    18781 accagtcagg atgctgattt tactgcgaaa actatcgccg ataagcaggc ggatacgaat
    18841 caggaacagg ctaaaacaga agacgcgaag cgccacgata aagagcaggt gtaatccgtg
    18901 tttgatatcg gttttagcga actgctattg gtgttcatca tcggcctcgt cgttctgggg
    18961 ccgcaacgac tgcctgtggc ggtaaaaacg gtagcgggct ggattcgcgc gttgcgttca
    19021 ctggcgacaa cggtgcagaa cgaactgacc caggagttaa aactccagga gtttcaggac
    19081 agtctgaaaa aggttgaaaa ggcgagcctc actaacctga cgcccgaact gaaagcgtcg
    19141 atggatgaac tacgccaggc cgcggagtcg atgaagcgtt cctacgttgc aaacgatcct
    19201 gaaaaggcga gcgatgaagc gcacaccatc cataacccgg tggtgaaaga taatgaagct
    19261 gcgcatgagg gcgtaacgcc tgccgctgca caaacgcagg ccagttcgcc ggaacagaag
    19321 ccagaaacca cgccagagcc ggtggtaaaa cctgctgcgg acgctgaacc gaaaaccgct
    19381 gcaccttccc cttcgtcgag tgataaaccg taaacatgtc tgtagaagat actcaaccgc
    19441 ttatcacgca tctgattgag ctgcgtaagc gtctgctgaa ctgcattatc gcggtgatcg
    19501 tgatattcct gtgtctggtc tatttcgcca atgacatcta tcacttggta tccgcgccat
    19561 tgatcaagca gttgccgcaa ggttcaacga tgatcgccac cgacgtggcc tcgccgttct
    19621 ttacgccgat caagctgacc tttatggtgt cgctgattct gtcagcgccg gtgattctct
    19681 atcaggtgtg ggcatttatc gccccagcgc tgtataagca tgaacgtcgc ctggtggtgc
    19741 cgctgctggt ttccagctct ctgctgtttt atatcggcat ggcattcgcc tactttgtgg
    19801 tctttccgct ggcatttggc ttccttgcca ataccgcgcc ggaaggggtg caggtatcca
    19861 ccgacatcgc cagctattta agcttcgtta tggcgctgtt tatggcgttt ggtgtctcct
    19921 ttgaagtgcc ggtggcaatt gtgctgctgt gctggatggg gattacctcg tcagaagact
    19981 tacgcaaaaa acgcccgtat gtgctggttg gtgcattcgt tgtcgggatg ttgctgacgc
    20041 cgccggatgt cttctcgcaa acgctattgg cgatcccgat gtactgtctg tttgaaatcg
    20101 gtgtcttctt ctcacgcttt tacgttggta aagggcgaaa tcgggaagag gaaaacgacg
    20161 ctgaagcaga aagcgaaaaa actgaagaat aaattcaacc gcccgtcagg gcggttgtca
    20221 tatggagtac aggatgtttg atatcggcgt taatttgacc agttcgcaat ttgcgaaaga
    20281 ccgtgatgat gttgtagcgc gcgcttttga cgcgggagtt aatgggctac tcatcaccgg
    20341 cactaacctg cgtgaaagcc agcaggcgca aaagctggcg cgtcagtatt cgtcctgttg
    20401 gtcaacggcg ggcgtacatc ctcacgacag cagccagtgg caagctgcga ctgaagaagc
    20461 gattattgag ctggccgcgc agccagaagt ggtggcgatt ggtgaatgtg gtctcgactt
    20521 taaccgtaac ttttcgacgc cggaagagca ggaacgcgct tttgttgctc agctacgcat
    20581 tgccgcagaa ttaaacatgc cggtatttat gcactgtcgc gatgcccacg agcggtttat
    20641 gacattgctg gagccgtggc tggataaact gcctggtgcg gttcttcatt gctttaccgg
    20701 cacacgcgaa gagatgcagg cgtgcgtggc gcgtggaatt tatatcggca ttaccggttg
    20761 ggtttgcgat gaacgacgcg ggctggagct gcgggaattg ttgccgttga ttccggcgga
    20821 gaaattgctg atcgaaacag atgcgccgta tctgctcccg cgcgatctca cgccaaagcc
    20881 atcatcccgg cgcaacgagc cagcccatct gccccatatt ttgcaacgta ttgcgcactg
    20941 gcgtggagaa gatgccgcat ggctggctgc caccacggat gccaatgtca aaacactgtt
    21001 tgggattgcg ttttagagtt tgcggaactc ggtgttcttc acactgtgct taatctcttt
    21061 attaataaga ttaagcaata gcatggagcg agcctcacca tcgggttcgg tgaaaatggc
    21121 ctgaaagcct tcgaacgcgc cttcggtaat aatcacctta tcacccggat aagggggggc
    21181 cggatcgaca atgtctttcg gtttatatac cgatagctga tgaattaccg ccgatgggac
    21241 tatcgctggc gacgcgccaa agcgcacaaa gtggctgacg ccacgcgtag cgctgatggt
    21301 cgtggtatga atcacttctg gatcaaattc cacaaacagg tagttaggga ataacggttc
    21361 actgactgcg gtacgtttac cccgtacgat tttttccagg gtgatcatcg gagcgaggca
    21421 attaaccgcc tgtctctcaa ggtgttcctg ggcacgttga agttgcccgc gcttgcagta
    21481 cagtaaatac caggattgca taatgactct tatccgttta atcggggcgc aaggatagca
    21541 aaagctttac gctaagttaa ttatattccc cggtttgcgt tataccgtca gagttcacgc
    21601 taatttaaca aatttacagc attgcaaaga tgaacgccgt ataatgggcg cagattaaga
    21661 ggctacaatg gacgccatga aatataacga tttacgcgac ttcctgacgc tgcttgaaca
    21721 gcagggtgag ctaaaacgta tcacgctccc ggtggatccg catctggaaa tcactgaaat
    21781 tgctgaccgc actctgcgtg ctggtgggcc tgcgctgttg ttcgaaaacc ctaaaggcta
    21841 ctcaatgccg gtgctgtgca acctgttcgg tacgccaaag cgcgtggcga tgggcatggg
    21901 gcaggaagat gtttcggcgc tgcgtgaagt tggtaaatta ttggcgtttc tgaaagagcc
    21961 ggagccgcca aaaggtttcc gcgacctgtt tgataaactg ccgcagttta agcaggtgtt
    22021 aaacatgccg acaaagcgac tgcgtggtgc gccctgccaa caaaaaatcg tctctggcga
    22081 tgacgtcgat ctcaatcgca ttcccattat gacctgctgg ccggaagatg ccgcgccgct
    22141 gattacctgg gggctgaccg taacgcgcgg cccgcataaa gagcggcaga atctgggcat
    22201 ttatcgccag cagctgattg gtaaaaacaa actgattatg cgctggctgt cgcatcgcgg
    22261 cggcgcgctg gattatcagg agtggtgtgc ggcgcatccg ggcgaacgtt tcccggtttc
    22321 tgtggcgctg ggtgccgatc ctgccacgat tctcggtgca gtcacccccg ttccggatac
    22381 gctttcagag tatgcgtttg ccggattgct acgcggcacc aaaaccgaag tagtaaagtg
    22441 tatttccaat gacctcgaag tgccagccag tgccgaaatc gtcctggaag ggtatatcga
    22501 tcctggtgag atggcgccgg aagggccgta tggtgaccac acaggttact ataatgaagt
    22561 cgataatttc ccggtgttta ccgtgacgca tattacccag cgtgaagatg cgatttacca
    22621 ttccacctat accgggcgtc cgccagatga gcccgcggta ctgggcgtgg cgttgaacga
    22681 agtgtttgta ccgattctgc aaaaacagtt cccggaaatt gtcgattttt acctgccgcc
    22741 ggaaggctgt tcttatcgtc tggcggtagt gacgatcaaa aaacagtacg ccggacacgc
    22801 gaagcgcgtc atgatgggcg tctggtcgtt cttacgccag tttatgtaca ctaaatttgt
    22861 gatcgtttgc gatgatgacg tcaacgcacg cgactggaac gatgtgattt gggcgattac
    22921 cacccgtatg gacccggcgc gggatactgt tctggtagaa aatacgccta ttgattatct
    22981 ggattttgcc tcgcctgtct ctgggctggg ttcaaaaatg gggctggatg ccacgaataa
    23041 atggccgggg gaaacccagc gtgaatgggg acgtcccatc aaaaaagatc cagatgttgt
    23101 cgcgcatatt gacgccatct gggatgaact ggctattttt aacaacggta aaagcgcctg
    23161 atgcgcgttt gttttgccct atttatcgat ccgacagaga aagcgcatga caaccttaag
    23221 ctgtaaagtg acctcggtag cagctatcac ggataccgta tatcgtgtcc gcatcgtgcc
    23281 agacgcggcc ttttcttttc gtgctggtca gtatttgatg gtagtgatgg atgagcgcga
    23341 caaacgtccg ttctcaatgg cctcgacgcc ggatgaaaaa gggtttatcg agctgcatat
    23401 tggcgcttct gaaatcaacc tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca
    23461 tcaaatcgtg gtcgaccttc cccacggaga agcgtggctg cgcgatgatg aagagcgtcc
    23521 gatgattttg attgcgggcg gcaccgggtt ctcttatgcc cgctcgattt tgctgacagc
    23581 gttggcgcgt aacccaaacc gtgatatcac catttactgg ggcgggcgtg aagagcagca
    23641 tctgtatgat ctctgcgagc ttgaggcgct ttcgttaaag caccttggtc tgcaagtggt
    23701 gccggtggtt gaacaaccgg aagcaggctg gcgtgggcgt actggtaccg tgttaacggc
    23761 ggtattgcag gatcatggta cgctggcaga gcatgatatc tatattgccg gacgttttga
    23821 gatggcgaaa attgcccgtg acctgttttg cagtgagcgt aatgcgcggg aagatcgcct
    23881 gtttggcgat gcgtttgcat ttatctgaga tataaaaaaa cccgcccctg acaggcggga
    23941 agaacggcaa ctaaaacttt attcagtggc atttagatct atgtcgcatc cggcaattgg
    24001 tgcacgatgc ctgatgcgac gctggcgcgt cttatcaggc ctacattggt gccggatcgg
    24061 tagaccggat aaggcgttca cgccgcatcc ggcaagtggt taaacccgct caaacaccgt
    24121 cgcaataccc tgacccagac cgatacacat cgtcgccaga ccaaactgaa cgtctttgcg
    24181 ttccatcaga ttcagcagcg tggtgctgat acgcgcaccg gaacaaccca gcggatgacc
    24241 cagcgcgatc gcaccaccat tgaggttgat cttctcgtca atctgctcca ttagcccgag
    24301 atctttaatg cacggcagga tctgcgcggc aaaggcttcg ttcatctcaa acacgccgat
    24361 atcgctggca gaaagccccg cttttttcag cgccagcttc gaggccggaa ccgggccgta
    24421 acccataatc gatgggtcac aaccaacgac cgccatcgaa cgcacacgag cgcgcggctt
    24481 aagacctaat tcatgggcgc ggctttcact catcaccagc atggcagctg cgccatcgga
    24541 aagtgcagaa gatgtgcccg ccgttaccgt accgtttact ggatcaaacg ccggacgcag
    24601 cgtggcgagg gcttccacgg tggtttccgg gcgaatcact tcgtcgtaat taaactgctt
    24661 caggacgccg tcggcatcgt gaccaccagt tgggatgatt tcatttttaa atgcggccga
    24721 ctgcgtggcg gcccaggcgc gggcgtgtga ccgcgcggca aaggcatcct gcatttcacg
    24781 gctgataccg tgcatacgcg ccagcatttc tgccgttaag cccatcatgc ccgccgcttt
    24841 ggcgacattg cggctcaggc cggggtgaaa atcgacgccg tgactcatcg gcacatggcc
    24901 tatatgctcc acgccgccaa ccagacatgc ctgcgtatcg ccagtcatga tcattcgtgc
    24961 tgcgtcatgc agtgcctgca tggatgaacc acacaagcga ttgacggtaa ccgccgggac
    25021 agagtgcggc acttctgcca gcagcgccgc gttacgggcg atgttaaaac cctgctccag
    25081 cgtctgttgc acacaacccc agtaaatatc gtcgagggcc gccgcttcca gcgccgggtt
    25141 acgcgccagc aggctacgca ttaaatgagc ggagagatct tccgcacgca cgttacgaaa
    25201 agcaccgccc ttcgaacggc ccatcggggt gcgaattgca tcgacaatga caacctgttc
    25261 cattgtgact ccttaagccg ttttcaggtc gccaaccgga cgggctggct caaccggagg
    25321 atagtacggt tcgttatgac gcgctttatt acgcagacct tccggcactt catacagcgg
    25381 gccgaggtgc tgatattgct gtgccatatc gaggtatttt gcgctaccga gggtgtccag
    25441 ccagcggaac gcgccgccgt ggaacggagg gaagcccagg ccgtagacca gcgccatatc
    25501 cgcttccgcc ggagtggcga taatgccctc ttccagacag cgcaccactt cgttgaccat
    25561 cgggatcatc atgcgggcga taatctcttc ttcgctgaaa tcgcgcttcg gttggctcac
    25621 ttctgccagc aggtcttcaa cggcggcgtc ttcttctttc ttcggcttac ctttgctgtc
    25681 ttctttataa cgccagaaac cgaggccgtt cttctgacca aagcggttgg catcaaacag
    25741 cgcgtcgatg gcatcgcggt aatctttctg catccgctgc gggaagcctg ctgccatgac
    25801 agcctgagcg tgatgcgcgg tatcaatgcc cacaacgtcc agcagatatg ccgggcccat
    25861 cggccagcca aactgttttt ccatcacttt gtcgatcttg cggaaatccg cgccgtcgcg
    25921 cagcagctgg ctgaaaccgg cgaaatacgg gaacagcacg cggttaacaa agaagccggg
    25981 gcagtcgtta accacaatcg gcgtcttgcc catcttgctc gcccaggcga caactttcgc
    26041 gatggtttcg tcggagcttt tctcgccgcg aataatttct accaacggca ttcggtggac
    26101 cgggttaaag aagtgcatcc cgcagaagtt ttccgggcgt tccagcgcgt tggccagttc
    26161 gctgatagga atggttgaag tgttagacgc cagcacggta tcctggcgta ctttttgttc
    26221 ggtttctgcc agtacggctt ttttcacttt cgggttttca acaaccgctt ctaccacaat
    26281 atccacgcgg tcaaatccgg cgtagtcgag cgttgggtgg atcgtggaga tcacgccagc
    26341 cagtttcaga ccatcgattt tgccgcgctc aagctgcttg ttcagcagtt tcgcggcttc
    26401 ggtcatgccg agggttaacg acttgtcgtt gatatctttc atgacaaccg gcacgccttt
    26461 ccacgcggac tggtaagcga taccgccacc catgatgcct gcgcccagca cagcagcctg
    26521 tttcggggtt tcaacgtctt tggtgagttt cttcgctttg ccttttacat attgatcgtt
    26581 aaggaaaatg ccgaccagtg cgcgagcttc gttggtatgc gccagcggga caaaactttt
    26641 gttttccaga tttaaggctt cttcacgacc aaaacgggcc gcagcttcaa tggtttttac
    26701 tgcggtgatg ggggccggat aatgtttccc cgccgtttgc gcgaccattc ctttagcgat
    26761 ggtgaagctc atggtggctt caatcttgct cagttttagc ggttcctgct tcggctgacg
    26821 tttcgctttc cagtcgaggt cgccgttaat ggcctggcgt aaaatcgcca tcgccccttc
    26881 aatcagtttt tctgctttga ctacgccatc caccagaccg attttcagcg cctgatccgc
    26941 gccgacatct ttaccggcgg caatgatttc cagtgcactg tcagcgccca gcatacgtgg
    27001 catacgtaca gaaccgccaa agcccggcat gatgcccagt ttggtttccg gcagaccgat
    27061 gcgcagatcc ggcgtcgcca gacgataatc ggtcgccagc acgcattcgc agccaccacc
    27121 cagcgcatag ccgttgacgg cagcaatggt cggcaccggc agatcttcca ggcgattaaa
    27181 cacgctattg gcaaagtgca gccactgact taactgttct tcaggaacga ggaacaggga
    27241 caaaaattcg gtgatatcag caccgacgat aaaggctgct ttgttcgaac gcagcagcag
    27301 cccttttaga tctgattgct gttccagcac gccgatggcc tcgccgaggc tggcgacggt
    27361 cgcagtgtcg agtttattaa ctgaacctgg ggcatcaaat accagttcgg caatgccatc
    27421 ttccagccag tcaaggtaca gggtgtcgcc tttgtaaagc atgtcagtct cctgaatccg
    27481 caaggtaatc tggtcgtacc agatgcatcg gagtgtgtgt tttgtgttaa aaatatgcaa
    27541 ataaaagatt aaagaaatgc cgatctgatc acgctcggca gaaatcacgc tctggatgaa
    27601 cgatgtgcta agatgcggag acttaaggtc aaaaaaacag aagggtaaaa aatggaatca
    27661 ctggcctcgc tctataaaaa tcatatagct accttacaag aacggactcg cgatgcgctg
    27721 gcgcgcttca agctggatgc attacttatt cactccggcg agctgttcaa cgtttttctc
    27781 gacgatcatc cctatccgtt taaagtgaac ccgcaattca aagcgtgggt gccggtaact
    27841 caggtgccaa actgctggtt gctggtggat ggcgtgaaca agccgaaact gtggttctat
    27901 ctgccggttg attactggca caacgtcgaa ccgctgccga cctccttctg gactgaagat
    27961 gtggaagtga tcgcgctgcc gaaagccgat ggcattggta gcctgctgcc tgctgcgcgc
    28021 ggcaatatcg gttatatcgg tccggtgccg gaacgtgcgc tgcaactggg tattgaggcc
    28081 agcaacatca acccgaaagg ggtgatcgac tacctgcatt attaccgctc cttcaaaacc
    28141 gagtacgaac tggcctgtat gcgtgaagcg cagaaaatgg cggtcaatgg tcatcgtgcg
    28201 gcagaagaag cgttccgttc tggcatgagc gagttcgata tcaatattgc ctatctgacc
    28261 gcgaccggtc atcgtgatac cgacgtacct tacagcaaca ttgtggcgct taacgaacac
    28321 gctgcggtgt tgcattacac caaactggac caccaggcac cggaagagat gcgcagcttc
    28381 ctgctggatg ccggggccga atataacggc tatgcggctg acctgacccg tacctggtcg
    28441 gcaaaaagcg acaacgacta cgcacagctg gtgaaagacg taaatgatga acaactggcg
    28501 ctgatcgcca ccatgaaagc aggcgtcagc tatgtggatt accacatcca gttccatcag
    28561 cgcatcgcca aactgctgcg taaacatcaa atcatcaccg atatgagtga agaagcgatg
    28621 gtcgaaaacg atctcaccgg accgtttatg ccgcacggta ttggccatcc gctgggcctg
    28681 caggtgcatg acgtcgccgg ttttatgcag gatgatagcg gtacacacct cgcggcaccg
    28741 gcaaaatatc cgtacctgcg ctacacccgt attctccagc cgggcatggt gttaaccatc
    28801 gaaccgggta tctacttcat tgaatcgctt ctggcaccgt ggcgtgaagg gcagttcagc
    28861 aagcacttca actggcagaa aattgaagca ctgaaaccgt tcggcggcat tcgtatcgaa
    28921 gacaacgtgg tgatccacga aaacaacgtg gaaaacatga cccgggatct gaaactggcg
    28981 tgatggaaag ctggttaatt cctgcggcac cggtcacggt cgttgaagag atcaaaaaga
    29041 gccgcttcat aacgatgttg gcgcataccg atggcgttga ggcggcgaaa gcgtttgttg
    29101 aatcggtgcg ggcagaacac cccgatgccc gccaccattg cgtggcgtgg gtggcgggtg
    29161 cgccggatga ttctcaacag ctgggtttct ctgacgacgg ggagccggcg ggaacggcag
    29221 gtaaaccgat gctcgcccag ctaatgggca gcggcgtcgg ggaaattacc gctgtggtag
    29281 tgcgctacta cggcggcata ttgcttggca ccggtgggtt agtgaaagcg tatggcggcg
    29341 gcgtgaatca ggcgctgcgc cagctaacga cccaacgcaa gacgccatta accgaatata
    29401 ctttgcaatg tgaatatagt cagttaaccg gcattgaagc gttgctgggg cagtgtgacg
    29461 gcaaaattat caacagtgat tatcaggcat tcgttctgct gcgggtggcg cttccggcgg
    29521 cgaaagtggc tgaattttcc gcaaagctgg cggattttag ccgtggttca ttgcaattgt
    29581 tagcgattga agaataatcc ccacttcgtt ttgcagaact aaggaagcgg cagagatgca
    29641 ttttcgcgcc attacccgaa tcgttggact actggtcatc ttattttcag ggaccatgat
    29701 tatccctggg ctggtagcac tcatctaccg ggatggagcg ggccgcgctt ttacccagac
    29761 cttttttgtc gccctcgcca ttggctctat gctgtggtgg ccgaaccgca aagagaaggg
    29821 cgaactgaaa tcccgtgagg ggtttctgat agtggtgctg ttttggaccg tgctgggtag
    29881 tgtcggtgcg ctccctttta tcttctcgga aagcccgaac ctcacgatta ccgatgcgtt
    29941 ttttgaatct ttctctggcc tgaccaccac cggggccact acgctggtgg ggctggattc
    30001 gctccctcat gccatcctct tttatcgcca gatgctgcaa tggtttggcg ggatggggat
    30061 catcgtgtta gcggttgcga tactgcctat cctcggcgtg ggtgggatgc agctctatcg
    30121 cgcagaaatg cccggcccgc tgaaagataa caaaatgcgc ccgcgaattg cggaaacggc
    30181 gaaaactctg tggttgattt atgtcttgct gaccgtcgcc tgtgcgctgg cgttgtggtt
    30241 tgccggaatg gatgcctttg atgccatcgg ccatagcttt gcgactatcg ctattggcgg
    30301 cttctcgaca catgatgcca gtatcggtta tttcgacagt ccgactatta acactatcat
    30361 tgctatcttc ctgctgatct ccggctgtaa ctacggtctg cacttttcac tgttaagtgg
    30421 gcgtagtctg aaggtttatt ggcgcgatcc ggaatttcgc atgtttatcg gcgtacagtt
    30481 tacgctggtg gttatttgta cactcgtact gtggtttcat aatgtctaca gttcggcgct
    30541 gatgacaatt aaccaggcgt ttttccaggt ggtatcgatg gcgacaaccg ccgggtttac
    30601 gactgacagc attgcccgct ggccgctctt tttgccggta ctgcttttat gttcagcttt
    30661 tatcggcggt tgcgccgggt caacgggcgg tggcctgaaa gtgatccgca tcctgctgct
    30721 gtttaagcag gggaaccgtg agctgaaacg actggtgcat ccgaacgccg tctatagcat
    30781 taagctgggg aatcgcgcac tgccggaacg tatcctcgaa gccgtttggg gatttttctc
    30841 cgcctatgca ttggtgttta ttgtcagtat gctggcgatt atcgccacgg gcgtggatga
    30901 tttttctgcc tttgcctcag ttgttgcgac attgaataac ctggggccag ggcttggcgt
    30961 ggttgctgat aactttacca gtatgaaccc ggtggctaaa tggatcctga ttgccaacat
    31021 gctgtttggt cgtctcgagg tctttacatt actggtgctc tttaccccga ctttctggcg
    31081 tgaatgatgg agtaatacgt gaaaacatta attcttttct caacaaggga cggacaaacg
    31141 cgcgagattg cctcctacct ggcttcggaa ctgaaagaac tggggatcca gacggatgtc
    31201 gccaatgtgc accgcattga agaaccacaa tgggaaaact atgaccgtgt ggtcattggt
    31261 gcttctattc gctatggtca ctaccactct gcgttccagg aatttgtcaa aaaacatgcg
    31321 acgcgactga attcgatgcc gagcgccttt tattccgtaa acctggtggc gcgcaaaccg
    31381 gagaagcgta ctccacagac caacagctac gcgcggaagt ttctgatgaa ctcgcaatgg
    31441 cgtcccgatc gctgcgcggt cattgccggg gcgctgcgtt acccacgtta tcgctggtac
    31501 gaccgtttta tgctcaagct gattatgaag atgtcaggcg gtgaaacgga tacgcgcaaa
    31561 gaagttgtct ataccgattg ggagcaggtg gcgaatttcg cccgagaaat cgcccattta
    31621 accgacaaac cgacgctgaa ataagcataa agaataaaaa atgcgcggtc agaaaattat
    31681 tttaaatttc ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga
    31741 acaacggcaa acacgcagcc gggtcagcgg ggttctcctg agaattccga cagagaaagc
    31801 aaaaataaat gcttgacttt gtagcgggaa agcgtattat gaacaccccg cgccgctgag
    31861 aaaaagcgaa gcgg
//
LOCUS       DAZOZX010000034        31187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZOZX010000034 DAZOZX010000000
VERSION     DAZOZX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.034"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            389..2047
                     /locus_tag="ACHUPT_001545"
     CDS             389..2047
                     /locus_tag="ACHUPT_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0819166.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHUPT_001546"
     CDS             complement(2044..3000)
                     /locus_tag="ACHUPT_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0819167.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHUPT_001547"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHUPT_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0819168.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHUPT_001548"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHUPT_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0819169.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUPT_001549"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUPT_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0819170.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUPT_001550"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUPT_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0819171.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUPT_001551"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUPT_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0819172.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUPT_001552"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUPT_001552"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0819173.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHUPT_001553"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHUPT_001553"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUPT_001554"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUPT_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0819174.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUPT_001555"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUPT_001555"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0819175.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUPT_001556"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUPT_001556"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0819176.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUPT_001557"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUPT_001557"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0819177.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUPT_001558"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUPT_001558"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0819178.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUPT_001559"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUPT_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0819179.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUPT_001560"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUPT_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0819180.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUPT_001561"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUPT_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0819181.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUPT_001562"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUPT_001562"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0819182.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHUPT_001563"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHUPT_001563"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0819183.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUPT_001564"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUPT_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUPT_001565"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUPT_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0819184.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACHUPT_001566"
     CDS             28042..28833
                     /locus_tag="ACHUPT_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR0819185.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUPT_001567"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUPT_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0819186.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACHUPT_001568"
     CDS             29810..30958
                     /locus_tag="ACHUPT_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0819187.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31187
                     /locus_tag="ACHUPT_001569"
     CDS             30976..>31187
                     /locus_tag="ACHUPT_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0819188.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7690 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggtgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagga
//
LOCUS       DAZOZX010000035        30456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZOZX010000035 DAZOZX010000000
VERSION     DAZOZX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.035"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="ACHUPT_001570"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="ACHUPT_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0819189.1"
                     /translation="RAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLS"
     gene            608..991
                     /gene="secE"
                     /locus_tag="ACHUPT_001571"
     CDS             608..991
                     /gene="secE"
                     /locus_tag="ACHUPT_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0819190.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            993..1538
                     /gene="nusG"
                     /locus_tag="ACHUPT_001572"
     CDS             993..1538
                     /gene="nusG"
                     /locus_tag="ACHUPT_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0819191.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1697..2125
                     /gene="rplK"
                     /locus_tag="ACHUPT_001573"
     CDS             1697..2125
                     /gene="rplK"
                     /locus_tag="ACHUPT_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0819192.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2129..2833
                     /gene="rplA"
                     /locus_tag="ACHUPT_001574"
     CDS             2129..2833
                     /gene="rplA"
                     /locus_tag="ACHUPT_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0819193.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3246..3743
                     /gene="rplJ"
                     /locus_tag="ACHUPT_001575"
     CDS             3246..3743
                     /gene="rplJ"
                     /locus_tag="ACHUPT_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0819194.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3810..4175
                     /gene="rplL"
                     /locus_tag="ACHUPT_001576"
     CDS             3810..4175
                     /gene="rplL"
                     /locus_tag="ACHUPT_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0819195.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4495..8523
                     /gene="rpoB"
                     /locus_tag="ACHUPT_001577"
     CDS             4495..8523
                     /gene="rpoB"
                     /locus_tag="ACHUPT_001577"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0819196.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8600..12823
                     /gene="rpoC"
                     /locus_tag="ACHUPT_001578"
     CDS             8600..12823
                     /gene="rpoC"
                     /locus_tag="ACHUPT_001578"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0819197.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="ACHUPT_001579"
     CDS             complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="ACHUPT_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0819198.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14195..14965)
                     /gene="thiG"
                     /locus_tag="ACHUPT_001580"
     CDS             complement(14195..14965)
                     /gene="thiG"
                     /locus_tag="ACHUPT_001580"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0819199.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="ACHUPT_001581"
     CDS             complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="ACHUPT_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0819200.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="ACHUPT_001582"
     CDS             complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="ACHUPT_001582"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0819201.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="ACHUPT_001583"
     CDS             complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="ACHUPT_001583"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0819202.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="ACHUPT_001584"
     CDS             complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="ACHUPT_001584"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0819203.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18510..18610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="ACHUPT_001585"
     CDS             complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="ACHUPT_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR0819204.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19234..20007
                     /gene="nudC"
                     /locus_tag="ACHUPT_001586"
     CDS             19234..20007
                     /gene="nudC"
                     /locus_tag="ACHUPT_001586"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0819205.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20047..21111
                     /gene="hemE"
                     /locus_tag="ACHUPT_001587"
     CDS             20047..21111
                     /gene="hemE"
                     /locus_tag="ACHUPT_001587"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0819206.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21268..21939
                     /gene="nfi"
                     /locus_tag="ACHUPT_001588"
     CDS             21268..21939
                     /gene="nfi"
                     /locus_tag="ACHUPT_001588"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0819207.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21982..22572
                     /locus_tag="ACHUPT_001589"
     CDS             21982..22572
                     /locus_tag="ACHUPT_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0819208.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22759..23031
                     /gene="hupA"
                     /locus_tag="ACHUPT_001590"
     CDS             22759..23031
                     /gene="hupA"
                     /locus_tag="ACHUPT_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0819209.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23044..23739
                     /locus_tag="ACHUPT_001591"
     CDS             23044..23739
                     /locus_tag="ACHUPT_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0819210.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="ACHUPT_001592"
     CDS             complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="ACHUPT_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0819211.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24404..25780
                     /gene="zraS"
                     /locus_tag="ACHUPT_001593"
     CDS             24404..25780
                     /gene="zraS"
                     /locus_tag="ACHUPT_001593"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0819212.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25777..27102
                     /gene="zraR"
                     /locus_tag="ACHUPT_001594"
     CDS             25777..27102
                     /gene="zraR"
                     /locus_tag="ACHUPT_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0819213.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27099..28388)
                     /gene="purD"
                     /locus_tag="ACHUPT_001595"
     CDS             complement(27099..28388)
                     /gene="purD"
                     /locus_tag="ACHUPT_001595"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0819214.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28400..29989)
                     /gene="purH"
                     /locus_tag="ACHUPT_001596"
     CDS             complement(28400..29989)
                     /gene="purH"
                     /locus_tag="ACHUPT_001596"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0819215.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7138 a         7935 c         8381 g         7002 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggtcgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta
      361 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
      421 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      481 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      541 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      601 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      661 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      721 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      781 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      841 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      901 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      961 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
     1021 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
     1081 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
     1141 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
     1201 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
     1261 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
     1321 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1381 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1441 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1501 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1561 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1621 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1681 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1741 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1801 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1861 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1921 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1981 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     2041 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     2101 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     2161 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     2221 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     2281 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     2341 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2401 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2461 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2521 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2581 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2641 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2701 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2761 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2821 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2881 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2941 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     3001 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     3061 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     3121 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     3181 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     3241 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     3301 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3361 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3421 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3481 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3541 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3601 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3661 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3721 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3781 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3841 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3901 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3961 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     4021 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     4081 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     4141 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     4201 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     4261 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     4321 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4381 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4441 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4501 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4561 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4621 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4681 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4741 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4801 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4861 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4921 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4981 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     5041 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     5101 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     5161 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     5221 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     5281 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     5341 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5401 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5461 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5521 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5581 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5641 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5701 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5761 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5821 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5881 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5941 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     6001 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     6061 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     6121 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     6181 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     6241 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     6301 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6361 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6421 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6481 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6541 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6601 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6661 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6721 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6781 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6841 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6901 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6961 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     7021 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     7081 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     7141 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     7201 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     7261 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     7321 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7381 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7441 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7501 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7561 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7621 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7681 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7741 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7801 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7861 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7921 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7981 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     8041 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     8101 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     8161 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     8221 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     8281 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     8341 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8401 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8461 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8521 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8581 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8641 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8701 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8761 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8821 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8881 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8941 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     9001 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     9061 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     9121 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     9181 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     9241 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     9301 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9361 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9421 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9481 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9541 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9601 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9661 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9721 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9781 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9841 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9901 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9961 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
    10021 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
    10081 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
    10141 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
    10201 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
    10261 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
    10321 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10381 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10441 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10501 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10561 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10621 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10681 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10741 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10801 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10861 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10921 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10981 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    11041 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    11101 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    11161 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    11221 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    11281 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    11341 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11401 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11461 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11521 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11581 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11641 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11701 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11761 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11821 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11881 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11941 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    12001 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    12061 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    12121 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    12181 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    12241 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    12301 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12361 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12421 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12481 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12541 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12601 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12661 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12721 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12781 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12841 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12901 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12961 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    13021 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    13081 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    13141 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    13201 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    13261 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    13321 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13381 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13441 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13501 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13561 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13621 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13681 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13741 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13801 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13861 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13921 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13981 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    14041 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    14101 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    14161 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    14221 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    14281 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    14341 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14401 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14461 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14521 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14581 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14641 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14701 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14761 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14821 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14881 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14941 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    15001 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    15061 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    15121 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    15181 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    15241 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    15301 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15361 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15421 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15481 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15541 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15601 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15661 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15721 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15781 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15841 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15901 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15961 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    16021 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    16081 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    16141 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    16201 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    16261 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    16321 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16381 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16441 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16501 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16561 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16621 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16681 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16741 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16801 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16861 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16921 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16981 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    17041 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    17101 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    17161 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    17221 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    17281 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    17341 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17401 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17461 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17521 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17581 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17641 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17701 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17761 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17821 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17881 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17941 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    18001 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    18061 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    18121 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    18181 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    18241 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    18301 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18361 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18421 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18481 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18541 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18601 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18661 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18721 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18781 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18841 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18901 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18961 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    19021 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    19081 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    19141 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    19201 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    19261 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    19321 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19381 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19441 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19501 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19561 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19621 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19681 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19741 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19801 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19861 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19921 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19981 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    20041 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    20101 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    20161 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    20221 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    20281 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    20341 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20401 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20461 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20521 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20581 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20641 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20701 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20761 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20821 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20881 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20941 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    21001 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    21061 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    21121 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    21181 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    21241 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    21301 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21361 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21421 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21481 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21541 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21601 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21661 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21721 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21781 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21841 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21901 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21961 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    22021 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    22081 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    22141 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    22201 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    22261 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    22321 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22381 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22441 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22501 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22561 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22621 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22681 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22741 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22801 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22861 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22921 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22981 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    23041 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    23101 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    23161 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    23221 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    23281 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    23341 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23401 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23461 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23521 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23581 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23641 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23701 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23761 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23821 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23881 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23941 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    24001 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    24061 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    24121 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    24181 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    24241 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    24301 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24361 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24421 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24481 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24541 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24601 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24661 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24721 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24781 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24841 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24901 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24961 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    25021 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    25081 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    25141 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    25201 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    25261 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    25321 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25381 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25441 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25501 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25561 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25621 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25681 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25741 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25801 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25861 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25921 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25981 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    26041 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    26101 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    26161 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    26221 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    26281 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    26341 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26401 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26461 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26521 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26581 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26641 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26701 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26761 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26821 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26881 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26941 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    27001 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    27061 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    27121 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    27181 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    27241 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    27301 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27361 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27421 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27481 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27541 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27601 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27661 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27721 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27781 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27841 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27901 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27961 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    28021 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    28081 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    28141 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    28201 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    28261 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    28321 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28381 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28441 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28501 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28561 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28621 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28681 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28741 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28801 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28861 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28921 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28981 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    29041 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    29101 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    29161 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    29221 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    29281 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    29341 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29401 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29461 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29521 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29581 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29641 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29701 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29761 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29821 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29881 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29941 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    30001 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    30061 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    30121 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    30181 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    30241 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    30301 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30361 ttcggttgaa gagaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    30421 tacgccacct cgcgacagtg cgctaaagcg cgtcgc
//
LOCUS       DAZOZX010000036        30297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZOZX010000036 DAZOZX010000000
VERSION     DAZOZX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.036"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUPT_001597"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUPT_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR0819216.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUPT_001598"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUPT_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0819217.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUPT_001599"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUPT_001599"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0819218.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUPT_001600"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUPT_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUPT_001601"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUPT_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0819219.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUPT_001602"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUPT_001602"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0819220.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUPT_001603"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUPT_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0819221.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="ACHUPT_001604"
     CDS             7490..7639
                     /locus_tag="ACHUPT_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819222.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="ACHUPT_001605"
     CDS             7840..9423
                     /locus_tag="ACHUPT_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0819223.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="ACHUPT_001606"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="ACHUPT_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0819224.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="ACHUPT_001607"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="ACHUPT_001607"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0819225.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="ACHUPT_001608"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="ACHUPT_001608"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0819226.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..13624
                     /locus_tag="ACHUPT_001609"
                     /pseudo
     CDS             13382..13624
                     /locus_tag="ACHUPT_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13623..14564
                     /gene="mdtA"
                     /locus_tag="ACHUPT_001610"
                     /pseudo
     CDS             13623..14564
                     /gene="mdtA"
                     /locus_tag="ACHUPT_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14564..17686
                     /gene="mdtB"
                     /locus_tag="ACHUPT_001611"
                     /pseudo
     CDS             14564..17686
                     /gene="mdtB"
                     /locus_tag="ACHUPT_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17687..20752
                     /gene="mdtC"
                     /locus_tag="ACHUPT_001612"
     CDS             17687..20752
                     /gene="mdtC"
                     /locus_tag="ACHUPT_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0819227.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20753..22168
                     /locus_tag="ACHUPT_001613"
     CDS             20753..22168
                     /locus_tag="ACHUPT_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0819228.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22165..23568
                     /gene="baeS"
                     /locus_tag="ACHUPT_001614"
     CDS             22165..23568
                     /gene="baeS"
                     /locus_tag="ACHUPT_001614"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0819229.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23565..24287
                     /gene="baeR"
                     /locus_tag="ACHUPT_001615"
     CDS             23565..24287
                     /gene="baeR"
                     /locus_tag="ACHUPT_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0819230.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24478..24810
                     /locus_tag="ACHUPT_001616"
     CDS             24478..24810
                     /locus_tag="ACHUPT_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0819231.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24957..26318
                     /gene="yegQ"
                     /locus_tag="ACHUPT_001617"
     CDS             24957..26318
                     /gene="yegQ"
                     /locus_tag="ACHUPT_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0819232.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26660..26977)
                     /gene="yegR"
                     /locus_tag="ACHUPT_001618"
     CDS             complement(26660..26977)
                     /gene="yegR"
                     /locus_tag="ACHUPT_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR0819233.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27383..28282
                     /gene="yegS"
                     /locus_tag="ACHUPT_001619"
     CDS             27383..28282
                     /gene="yegS"
                     /locus_tag="ACHUPT_001619"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0819234.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNSLIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28364..29137)
                     /locus_tag="ACHUPT_001620"
     CDS             complement(28364..29137)
                     /locus_tag="ACHUPT_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0819235.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="ACHUPT_001621"
     CDS             complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="ACHUPT_001621"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0819236.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7012 a         8048 c         7827 g         7410 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggagcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 cattccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    13681 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    13741 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    13801 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    13861 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    13921 tcgatggtcg cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata
    13981 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    14041 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag
    14101 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    14161 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    14221 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    14281 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    14341 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    14401 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    14461 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    14521 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    14581 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    14641 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    14701 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    14761 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    14821 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    14881 aacattaccg ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    14941 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    15001 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    15061 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    15121 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    15181 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    15241 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    15301 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    15361 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    15421 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    15481 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    15541 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    15601 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    15661 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    15721 ggtgtttctc gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg
    15781 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    15841 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    15901 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    15961 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    16021 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    16081 acagaacagc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    16141 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    16201 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    16261 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    16321 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    16381 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    16441 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    16501 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat
    16561 cgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    16621 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    16681 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    16741 cgccagccgt ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg
    16801 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca
    16861 caataccgaa aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga
    16921 cggtggcgtg gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    16981 catcaaccat ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta
    17041 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    17101 ggatatcacc acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    17161 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    17221 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    17281 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    17341 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    17401 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    17461 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    17521 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    17581 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    17641 attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc
    17701 cctcttcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    17761 catactgggt ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    17821 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac
    17881 gccgctggag cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc
    17941 gctcggcagc acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    18001 gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    18061 ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    18121 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac
    18181 gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt
    18241 gcgcgtcggg ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    18301 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    18361 ccgctggcag atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat
    18421 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc
    18481 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    18541 ccgcaaactg ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc
    18601 agagttgcag gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    18661 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    18721 gattctggtg gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc
    18781 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    18841 taatctttcg ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt
    18901 ggtgctggaa aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct
    18961 gcaaggtact cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt
    19021 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    19081 gacgctttct gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat
    19141 gtgcggctgg atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg
    19201 ccgcatgttg gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca
    19261 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa
    19321 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag
    19381 tatttcgttt caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga
    19441 tccggcagtg gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat
    19501 gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    19561 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga
    19621 tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga
    19681 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga
    19741 actggcggac gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga
    19801 ccgcgacacc atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa
    19861 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    19921 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    19981 caataacgaa ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    20041 cccactatcg gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc
    20101 gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    20161 tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat
    20221 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    20281 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    20341 tggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    20401 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    20461 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    20521 gcgttttcgc ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt
    20581 attgtcgggc ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg
    20641 tctggtgatg agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    20701 ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga
    20761 tcttcccgac agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc
    20821 gctggacacc accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag
    20881 tccgttgcat atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc
    20941 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    21001 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    21061 ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt
    21121 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg
    21181 tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg
    21241 gcactggatc tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat
    21301 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct
    21361 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    21421 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    21481 gcacgccaga aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    21541 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    21601 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    21661 gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg
    21721 ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt
    21781 gtttatgact accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    21841 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    21901 ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag
    21961 tatcggcgtc accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt
    22021 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    22081 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat
    22141 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    22201 tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt
    22261 atcagttttg agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag
    22321 ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc
    22381 aacaacgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat
    22441 aaacccggcc cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa
    22501 aacaacaaag tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc
    22561 attctggtca acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc
    22621 cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc
    22681 ctggcaacgt tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg
    22741 gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta
    22801 acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc
    22861 acgctggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg
    22921 cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa
    22981 ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt
    23041 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    23101 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc
    23161 cgtggcctga aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac
    23221 cgtttaatgc agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    23281 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    23341 agtgcgccag gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    23401 gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac
    23461 attgttgaag cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc
    23521 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    23581 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    23641 tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    23701 tactggcgta tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    23761 gcaccgatgg cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga
    23821 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    23881 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    23941 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg
    24001 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    24061 ttcgtctgct taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc
    24121 tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta
    24181 aaaatctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    24241 acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt
    24301 actttgttag ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc
    24361 agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc
    24421 agtaaacagc acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg
    24481 gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg
    24541 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    24601 ggcatcgcgt cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca
    24661 agcaacggca aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc
    24721 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    24781 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc
    24841 cggcgcttta ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc
    24901 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    24961 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    25021 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg
    25081 aattcaacca cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    25141 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    25201 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    25261 tgctggtgcg tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg
    25321 tgaactgggc gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc
    25381 gcgagctgtc gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg
    25441 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    25501 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    25561 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    25621 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    25681 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    25741 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg
    25801 gcgtgcattc gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg
    25861 cacaggttta ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc
    25921 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    25981 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    26041 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata
    26101 aattctccgt tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc
    26161 ttgagcacat ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata
    26221 ctgtgtggct cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt
    26281 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    26341 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    26401 tcgctgaaaa acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    26461 gtaatctccc ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct
    26521 gataaccgtc ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca
    26581 cgtaacggta tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga
    26641 cgcttacaaa aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta
    26701 atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga
    26761 aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag
    26821 aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg
    26881 caagaccaaa tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa
    26941 aaatactaat taatgatata gcagctatct ttttcatctc accttccccc attaaatacc
    27001 aacgacactc tctagtgttt aaatataata atggcatgat tattataatt gaataggatt
    27061 ataataaatg ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct
    27121 tcagggtaac tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa
    27181 tattaggtat atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc
    27241 cattgcaagc tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct
    27301 ccgactttca tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc
    27361 aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg
    27421 taaaagtact gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat
    27481 gacgatccat gtgcgggtca cctgggagaa aggtgatgcc gcacgatttg tagaggaggc
    27541 ccggaagttg ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt
    27601 ttctacggcg ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt
    27661 aggaaccgcc aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct
    27721 gaaactggca attgccggta atgccattgc gatagatatg gcgcaggtca acaaacaaac
    27781 ctgttttatt aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga
    27841 aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga
    27901 tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc
    27961 cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa
    28021 ctcgttaatt aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc
    28081 ggctctcgta tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc
    28141 gtcgtggttt gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt
    28201 gagtgggcaa aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc
    28261 agattgtcca ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat
    28321 gccggatgcg gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt
    28381 ttacccgtgt cttctgcaat gccaatttat ccacctctga cacagcatca tcggtaacaa
    28441 taatgtcaat tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg
    28501 aatcgagaac ggcaatcact ttatttgcgg cggtgaccat cgccccgcta atcgaataac
    28561 cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta
    28621 aatcggcatt aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt
    28681 tatggcggac cgtgccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa
    28741 acgctgccgg gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg
    28801 cgataagcat tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt
    28861 tgactgccgc ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa
    28921 acccgacttc ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat
    28981 gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt
    29041 ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta
    29101 attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg
    29161 tggctagtgt aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc
    29221 agcatcacgc tggcccgcgg tttcatggaa tgagcaacac tttgccctgc atggcattac
    29281 gagcgatgtc acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg
    29341 gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg
    29401 gccaagggct ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt
    29461 tgccaaacgt tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt
    29521 gggcatgagg accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct
    29581 cgaggataag ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca
    29641 tttcgctact gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg
    29701 agctaatgtc gatcgccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca
    29761 gcaggccaat ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta
    29821 aatgaaaagc atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaataggca
    29881 tatccgtggg tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac
    29941 catcacgccg cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac
    30001 actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta
    30061 aatcatcaac accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta
    30121 ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg
    30181 agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg
    30241 caacgcgcac gataccatca gtatcattca ctactgattt cataaaaact cctgatt
//
LOCUS       DAZOZX010000037        29746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZOZX010000037 DAZOZX010000000
VERSION     DAZOZX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.037"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHUPT_001622"
     CDS             <1..1224
                     /locus_tag="ACHUPT_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0819237.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUPT_001623"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUPT_001623"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0819238.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUPT_001624"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUPT_001624"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR0819239.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHUPT_001625"
     CDS             2714..3427
                     /locus_tag="ACHUPT_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0819240.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHUPT_001626"
     CDS             complement(3399..4076)
                     /locus_tag="ACHUPT_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0819241.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHUPT_001627"
     CDS             complement(4070..4747)
                     /locus_tag="ACHUPT_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0819242.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHUPT_001628"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHUPT_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHUPT_001629"
     CDS             complement(5443..6021)
                     /locus_tag="ACHUPT_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0819243.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHUPT_001630"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHUPT_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHUPT_001631"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHUPT_001631"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0819244.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUPT_001632"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUPT_001632"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0819245.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUPT_001633"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUPT_001633"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0819246.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUPT_001634"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUPT_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0819247.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHUPT_001635"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHUPT_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0819248.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHUPT_001636"
     CDS             12291..13031
                     /locus_tag="ACHUPT_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0819249.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHUPT_001637"
     CDS             13198..14148
                     /locus_tag="ACHUPT_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0819250.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUPT_001638"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUPT_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUPT_001639"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUPT_001639"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0819251.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUPT_001640"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUPT_001640"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0819252.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUPT_001641"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUPT_001641"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0819253.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUPT_001642"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUPT_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0819254.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUPT_001643"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUPT_001643"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0819255.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHUPT_001644"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHUPT_001644"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0819256.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUPT_001645"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUPT_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0819257.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHUPT_001646"
     CDS             23768..23827
                     /locus_tag="ACHUPT_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0819258.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHUPT_001647"
     CDS             complement(23928..24530)
                     /locus_tag="ACHUPT_001647"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0819259.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHUPT_001648"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUPT_001648"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUPT_001649"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUPT_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0819260.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUPT_001650"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUPT_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0819261.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUPT_001651"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUPT_001651"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0819262.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUPT_001652"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUPT_001652"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0819263.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUPT_001653"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUPT_001653"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0819264.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..>29746)
                     /locus_tag="ACHUPT_001654"
     CDS             complement(29676..>29746)
                     /locus_tag="ACHUPT_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819265.1"
                     /translation="KSKNHGIRKYNAKTEVKLVYFK"
BASE COUNT         7102 a         7438 c         8027 g         7179 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg actgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttag
//
LOCUS       DAZOZX010000038        29250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZOZX010000038 DAZOZX010000000
VERSION     DAZOZX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.038"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHUPT_001655"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHUPT_001655"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0819266.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUPT_001656"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUPT_001656"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0819267.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUPT_001657"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUPT_001657"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0819268.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUPT_001658"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUPT_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0819269.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUPT_001659"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUPT_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0819270.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUPT_001660"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUPT_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0819271.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUPT_001661"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUPT_001661"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0819272.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHUPT_001662"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHUPT_001662"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0819273.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUPT_001663"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUPT_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0819274.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUPT_001664"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUPT_001664"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0819275.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUPT_001665"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUPT_001665"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0819276.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHUPT_001666"
     CDS             8212..9075
                     /locus_tag="ACHUPT_001666"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0819277.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUPT_001667"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUPT_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0819278.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHUPT_001668"
     CDS             10411..11028
                     /locus_tag="ACHUPT_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0819279.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUPT_001669"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUPT_001669"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0819280.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUPT_001670"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUPT_001670"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0819281.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUPT_001671"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUPT_001671"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0819282.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUPT_001672"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUPT_001672"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0819283.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUPT_001673"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUPT_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0819284.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHUPT_001674"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHUPT_001674"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0819285.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUPT_001675"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUPT_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0819286.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHUPT_001676"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHUPT_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0819287.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHUPT_001677"
     CDS             21859..23568
                     /locus_tag="ACHUPT_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0819288.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHUPT_001678"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHUPT_001678"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0819289.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHUPT_001679"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHUPT_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHUPT_001680"
     tRNA            27625..27719
                     /locus_tag="ACHUPT_001680"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHUPT_001681"
     CDS             27991..29202
                     /locus_tag="ACHUPT_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0819290.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT         7229 a         7836 c         7340 g         6845 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc
//
LOCUS       DAZOZX010000039        28914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZOZX010000039 DAZOZX010000000
VERSION     DAZOZX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.039"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUPT_001682"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUPT_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0819291.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHUPT_001683"
     CDS             complement(2138..2584)
                     /locus_tag="ACHUPT_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0819292.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHUPT_001684"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHUPT_001684"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0819293.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUPT_001685"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUPT_001685"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0819294.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHUPT_001686"
     CDS             complement(5347..6009)
                     /locus_tag="ACHUPT_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0819295.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHUPT_001687"
     CDS             6182..6595
                     /locus_tag="ACHUPT_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0819296.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUPT_001688"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUPT_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0819297.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUPT_001689"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUPT_001689"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR0819298.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUPT_001690"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUPT_001690"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0819299.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUPT_001691"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUPT_001691"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0819300.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUPT_001692"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUPT_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0819301.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHUPT_001693"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHUPT_001693"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUPT_001694"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUPT_001694"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0819302.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUPT_001695"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUPT_001695"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0819303.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUPT_001696"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUPT_001696"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR0819304.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUPT_001697"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUPT_001697"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0819305.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUPT_001698"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUPT_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0819306.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHUPT_001699"
     CDS             14984..15841
                     /locus_tag="ACHUPT_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0819307.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHUPT_001700"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHUPT_001700"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0819308.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHUPT_001701"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHUPT_001701"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0819309.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUPT_001702"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUPT_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0819310.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHUPT_001703"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHUPT_001703"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0819311.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUPT_001704"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUPT_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUPT_001705"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUPT_001705"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0819312.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHUPT_001706"
     CDS             complement(22884..24023)
                     /locus_tag="ACHUPT_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0819313.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHUPT_001707"
     CDS             complement(24069..26165)
                     /locus_tag="ACHUPT_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0819314.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHUPT_001708"
     CDS             complement(26165..26911)
                     /locus_tag="ACHUPT_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0819315.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUPT_001709"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUPT_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0819316.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHUPT_001710"
     CDS             complement(27659..27964)
                     /locus_tag="ACHUPT_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819317.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHUPT_001711"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHUPT_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0819318.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7320 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZOZX010000040        28670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZOZX010000040 DAZOZX010000000
VERSION     DAZOZX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.040"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            164..649
                     /locus_tag="ACHUPT_001712"
     CDS             164..649
                     /locus_tag="ACHUPT_001712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819319.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            646..1032
                     /locus_tag="ACHUPT_001713"
     CDS             646..1032
                     /locus_tag="ACHUPT_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819320.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1057..3270
                     /locus_tag="ACHUPT_001714"
     CDS             1057..3270
                     /locus_tag="ACHUPT_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR0819321.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3293..3738
                     /locus_tag="ACHUPT_001715"
                     /pseudo
     CDS             3293..3738
                     /locus_tag="ACHUPT_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3760..7318
                     /locus_tag="ACHUPT_001716"
                     /pseudo
     CDS             3760..7318
                     /locus_tag="ACHUPT_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            7538..7741
                     /locus_tag="ACHUPT_001717"
     CDS             7538..7741
                     /locus_tag="ACHUPT_001717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819322.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7759..8079
                     /locus_tag="ACHUPT_001718"
     CDS             7759..8079
                     /locus_tag="ACHUPT_001718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819323.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8145..8377)
                     /locus_tag="ACHUPT_001719"
                     /pseudo
     CDS             complement(8145..8377)
                     /locus_tag="ACHUPT_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACHUPT_001720"
     CDS             complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACHUPT_001720"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0819324.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9438..9884
                     /gene="ivy"
                     /locus_tag="ACHUPT_001721"
     CDS             9438..9884
                     /gene="ivy"
                     /locus_tag="ACHUPT_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0819325.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACHUPT_001722"
     CDS             complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACHUPT_001722"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0819326.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12611..13189
                     /gene="lpcA"
                     /locus_tag="ACHUPT_001723"
     CDS             12611..13189
                     /gene="lpcA"
                     /locus_tag="ACHUPT_001723"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0819327.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13395..14162
                     /locus_tag="ACHUPT_001724"
     CDS             13395..14162
                     /locus_tag="ACHUPT_001724"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0819328.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACHUPT_001725"
     CDS             complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACHUPT_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0819329.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15257..15778
                     /locus_tag="ACHUPT_001726"
                     /pseudo
     CDS             15257..15778
                     /locus_tag="ACHUPT_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15954..16451
                     /locus_tag="ACHUPT_001727"
     CDS             15954..16451
                     /locus_tag="ACHUPT_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0819330.1"
                     /translation="MSEYRRYYIKGGTWFVTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16668..18380)
                     /locus_tag="ACHUPT_001728"
                     /pseudo
     CDS             complement(16668..18380)
                     /locus_tag="ACHUPT_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18352..19137
                     /gene="lafU"
                     /locus_tag="ACHUPT_001729"
     CDS             18352..19137
                     /gene="lafU"
                     /locus_tag="ACHUPT_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0819331.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19208..20263
                     /gene="dinB"
                     /locus_tag="ACHUPT_001730"
     CDS             19208..20263
                     /gene="dinB"
                     /locus_tag="ACHUPT_001730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0819332.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRECRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20315..20608
                     /gene="yafN"
                     /locus_tag="ACHUPT_001731"
     CDS             20315..20608
                     /gene="yafN"
                     /locus_tag="ACHUPT_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR0819333.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20611..21009
                     /gene="yafO"
                     /locus_tag="ACHUPT_001732"
     CDS             20611..21009
                     /gene="yafO"
                     /locus_tag="ACHUPT_001732"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR0819334.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21019..21471
                     /locus_tag="ACHUPT_001733"
     CDS             21019..21471
                     /locus_tag="ACHUPT_001733"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0819335.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21717..22760
                     /locus_tag="ACHUPT_001734"
     CDS             21717..22760
                     /locus_tag="ACHUPT_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0819336.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22822..23436
                     /gene="prfH"
                     /locus_tag="ACHUPT_001735"
                     /pseudo
     CDS             22822..23436
                     /gene="prfH"
                     /locus_tag="ACHUPT_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACHUPT_001736"
     CDS             complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACHUPT_001736"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0819337.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25212..25670
                     /gene="gpt"
                     /locus_tag="ACHUPT_001737"
     CDS             25212..25670
                     /gene="gpt"
                     /locus_tag="ACHUPT_001737"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0819338.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25762..27006
                     /gene="frsA"
                     /locus_tag="ACHUPT_001738"
     CDS             25762..27006
                     /gene="frsA"
                     /locus_tag="ACHUPT_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0819339.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27064..27465
                     /gene="crl"
                     /locus_tag="ACHUPT_001739"
     CDS             27064..27465
                     /gene="crl"
                     /locus_tag="ACHUPT_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0819340.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACHUPT_001740"
     CDS             complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACHUPT_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0819341.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7083 a         7122 c         7607 g         6858 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg cctgaaacta aatttacttt attgaatgta accaaatgaa atttttctgt
      661 attatagtga ttttcctttc aataaattac atagctatag tgaatgtttt caagtcttct
      721 gcagttgctc aatccaccaa atattatgaa gaaaatgata tacatgatgg aagtaatgac
      781 atctttttta tgaagaaata tgggccgcct gctcatgagt tcgtgaggcc acgatataaa
      841 tttgttggtg agtattatat cggcttgaat caaacttatc gcataacaga tcctgaattt
      901 caagatgttc ctattaagga aatgttctgg aatttgcagg gggatttgaa tctgacatgt
      961 tggtttcatt ataaagacgg gcaatggaga gtgattactt atattttttg gccaccaggg
     1021 gctagatttt aatccggtat taaaggagca aatatcatga gttttgtatc tacgggtaat
     1081 aaaccagttg gaaacggtgg acccgtcatc accacaccac caatagctgg cgaaagtggc
     1141 ggtatgagca caggttcagc tgtaaccgat gtatctggtg ccgccgagga gatggcagaa
     1201 caggctgcgg cggacctgtt tggtgcgctg ccggaaccgt caggtctggt taaagccgca
     1261 gtagctgctg cgcaggctgc cgctgcagca gcagggatct cggatatggc tggtgcagtt
     1321 caggatgcag cggcgtcgct tgcagccggt gctccggggg cgcataacgt cacagtaagt
     1381 ggtagtgcgg tgccgccgca gatgcttttg tttgcaggca tgaatggttc ggaagggctt
     1441 gggaatctgt tttcttacac agtacagctt aaaacgctgg acgccctgaa cctggggtac
     1501 gtttcccccg ctgctaacct gccgcttcag ctcatggtgg gcaaagacct gtgcgtcagt
     1561 atcgaactgg acggcggcgg aaaacggtat atcagcgggc tggtgacggc ggcgagggtg
     1621 gtcggccatg agagccgttc ggtaacgtat gaattacgca ttgagccatg gctgaagctg
     1681 ctgacccata ccagcgacta caaagcgttc cagaacaaaa ccgtggtgga tattctcgat
     1741 gaagtactgg atgaatattc ctttccggtg gaaaagcggc tggtggaaaa ttatccaccc
     1801 cgcgcctggc aggtgcagta tggtgaaacc gacttcgatt tcatccagcg cctgatgcag
     1861 gaatggggga tttactggtg gtttgagcac agcgagaaca gccacacgct ggtgctggtg
     1921 gatgccatca atggtcataa agcgtgccct gactcgccgc tggtggagtg gcaccaggaa
     1981 gggctgaaac tggataagga atttatccac accattacgg ccagtgagag gctgcgtact
     2041 ggaaagtggg tgatggacga ttttgatttt atgaagcctc gttcgctgct taaaagtacc
     2101 gtggccagtc cgcgtgatac cggtcatgct gaatacgagc attacgaatg gcctggtgat
     2161 tacttcacta cgggcgaagg cgagatgctg acgcgcattc gtatggaagc gcagcgcagc
     2221 ccaggcagcc gggcacatgg ggccggtcat atccgtaccc tcatgaccgg gtacaccttt
     2281 acgttgatga atcacccgac ggcagaaatt aatcaggaat atctgctggt acagaccact
     2341 ctgtttttac gggataatgc gcagcacagc ggtcaggatc agcattttac ctatgtcacc
     2401 acgtttgaac tgcaccccac cagcgaagtt taccgcccac aacgaaccct gagcaaaccc
     2461 cacaccaaag gcccgcagag cgccatcgtc accggcccgg cggggcagga aatctggacg
     2521 gataaatatg gccgggtgaa ggtgcagttt ggctgggacc gttacggaaa gaatgacgaa
     2581 aacagttcct gctggatccg cgtcagctat ccgtgggcgg gtaaaggatt cggcatgatc
     2641 cagatcccgc gtattggcca ggaagtactg gtggatttca aaaacggcga tccggatttg
     2701 cccatcatcg tggggcgcac ctataaccag gacaccatgc cgccgtgggg gctgccgggc
     2761 atggcgtcgc agagtgggat attcagccac tcattgcagg gagggccgac taacggaaat
     2821 atgctgcgct tcgatgataa aacgggtgcg gaagaggtga agttccatgc ggaaaaagac
     2881 ctcaatacca cggtaaaaaa caatgaaacc cacacggtca tggtcgatcg cataaaaacc
     2941 attgtcaaaa atgaaaccat cagcgtggga gaagaccgta atgccaccat cacaaaaaat
     3001 gatggtcttt ccgtcaaact ggctcaaacc atcaatgtcg gaacgacata tcgtctggat
     3061 gttggcgatc aatttaccct tcgttgcggc aatgcagcgc ttgtattaca caaggatggc
     3121 tcgattgagt tttgcggaaa acaactgtta ctgcacacca gcgatgttat gcaactcatt
     3181 ggcagtggca ttgatatgaa tccggatggc ggcacagcca tgactgcgga tgatattgcc
     3241 ccgttaccta cccccgacgt ttctgaataa aaaatataaa aggaattttt ctatgaccag
     3301 cctttaccgt attcaggagg gctgctttgc cctgcctgaa acttttcttg atcgcaccgt
     3361 caatatcttc gtcccatcgg gcaatgaaag ggcaactccc agtctgaata ttttccgcga
     3421 tacgctcagg ccagatgaaa atctgaccac ctatattgac cgccagatag ccttgatgaa
     3481 aaaaaaaacc taggccagca ccgggtactt tcgcggggaa cggcactggc gggaactggc
     3541 gaagctgcca tgacaggtga acagattctc gcaactcatc gttctggaaa aacagaagtg
     3601 taccagcgtc aggccgggtt tatcaccggg ccagcaaagg tactaatgtt gacgctgacc
     3661 actcagcgtc cttttgatga tcacaccgac cagctatgga ctgcatggtt gactagtttt
     3721 cagcctgcta aaagctaaaa aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg
     3781 gatgacccca tctaccacac cagcgccctt gccgggtttt tgataggggc catcataggt
     3841 atcgccatta ttgccgtcgc cagctttgct ttttttacct gcggcttttt ggccgggctg
     3901 attttgagtt ttctggctga tcagatagct tccggagtcc tgcaactggg tgaggctatt
     3961 gggcgctcga tacattccac agtgggaaaa attctgaccg gttctgataa tgtcagcacc
     4021 aacagtcgcc cggccgcccg ggccgtactg agtacggtga tatgcgaaga ccattccccg
     4081 gaaatccgca ttgcccaagg ttccggcaac atctacatca acagccagct gcccgtaagg
     4141 atgaccacac tgagtgtgat gcagtgattg aggacggctc ccccaatgtc tttctcggcg
     4201 gcggtacgca gacggtactg gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg
     4261 tggatgtgct gtatgtggtc gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg
     4321 cggcaaagat ggggtcgaaa tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa
     4381 tggtcgggat ggccatctct gacacagtca tgggattgtt cagcaatccg gtagatgtca
     4441 ccactgggca aaaaattttg ctgccggaga cggattttac cctgccagga cgcctgcctg
     4501 tcacctgttc gcgcttttac gccagccacc tggaaactga aggattgctg ggcagaggat
     4561 ggcggctgaa ctgggagata aacctgcgtg aggatgaaac atatatcacc ttcatcggtg
     4621 tacagggtcg ggaactgagt tatccgaaag agatgctgat accgggccac cagatattcg
     4681 acccggaaga gcagttttac ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca
     4741 ccgactgcag ctattacgtg tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt
     4801 ttatggagac gccgtaccgg cagcgtattg ctttcgggcg tgagaacggc aggctggtgc
     4861 gggtggcctc cagcagcgga caccatctgt tattgcaccg cacgatgacg caagcagggg
     4921 aacgcctgtc gcatattgaa cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc
     4981 gttacgacga taacgggcaa ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc
     5041 agtttgccta tgaaaatggc ctgatgacag agcatcgcaa cgcgacgggc tttacctgta
     5101 cctaccactg ggaagagatt gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg
     5161 gcgaggatta ccggttccac tatgattttg ctggcgggca gacggtggta accggcaggc
     5221 cggagcagaa gtggcagtgg tggtttgatg aagagacgta tgtgactgcg caccggacgc
     5281 cgggcggcgg aatgtaccgc ttcacgtaca acgaaaacca ttttccggta gcggtggaac
     5341 tccccggcga acgcagggta acgctggaat acgacaccct gagccgggtg gtgaaagaga
     5401 cggacccggc aggccgggtg acacagacgc agtggaacgg cagttttgct gaaatcacgc
     5461 gccgggcgct ggatgacgac cacgtatgga aggcggatta caacgaacac ggccaggtga
     5521 tacgggagac ggacccggaa gggcgcgtca cgcggtacgg gtatgatgat caggggctgc
     5581 cggagacggt ctgccacccc ggtaaacagc aggaccgtta cacctggaac gcgctggggt
     5641 tactgagcag ccaccgtcgc atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg
     5701 ggatgctggc gcgccatacg gatgaagaga aacgggagac gcgctggcag tacaccccgg
     5761 aagggctggt tgccagtctg agcaacggca acggggctca gtaccggttc agctacgacg
     5821 gcgacggacg gctgacggga gaacagcgcc cggacggcct gataaggatg tttgcgctga
     5881 atgcggacgg gtttccggtc atcatcccga cgcagggcac agagggcggg gtgcgcaatg
     5941 aacagcagga gcgtgatgcg ctgggccgcc tgctgcgcag tgacacgcag cacagtacac
     6001 ggaccttcag ctacaaccgt cttgaccaga taacggaagt gacgctcacc cccacggaag
     6061 aaggggaacg gctgcaccac atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg
     6121 ggtggctgac ggcagagcac agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg
     6181 gcaatcccac cgatatcacg ttgccggacg ggcagcatct cagtcatctt tactacggca
     6241 gcgggcatct gctccagacg gcactggacg gtatcacggt cagtgaatat gagcgtgaca
     6301 gcctgcaccg tcaggtgata cgcacgcagg ggaaactggc gacgttcagc ggctacaacg
     6361 ccgacaaccg gttaagctgg cagcgcagcc tgccaggtgg cagccagaat ccacagcagg
     6421 cggtactccc ccggcgcaga acgacagcgt gacggcgcgg gactatatct ggaacgcaga
     6481 cggtgaagtc ggcggcatca atgacaaact gcgcggttgc ctggtgttca gctatgaccg
     6541 cagcggctgg ctgaccagcc gcacggggca gatgtatgac catgaccatt attattacga
     6601 caaagcgggc aacctgctga cggatgagta tcagggagcg gtgatggata accgcctgcc
     6661 gggttacggg cgtgaccgct accgctataa cgaatggggc gaactgacag agcgcaggga
     6721 ccagcagctg gagtggaacg cgcagggcca gttaacgcgg gtcatcagca gcaacagcga
     6781 gacgcggtat cagtacgatg cgctgggcag gcgcatcagc aaggcgacca gcaatctcca
     6841 taccgaccgg ggagaacgaa gccggacaac gtttgtgtgg gaagggttcc ggctgttaca
     6901 ggagacaacg tggcagggaa agcggacata cctgtacgat gcggaacagc cgtacacgcc
     6961 ggtggctgcc atcacggggc gcggagagag ccagaaaatc tggtattacc acacggacct
     7021 gacgggcacg gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg cgggctacca
     7081 ggcgggattt ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact ttgagcagcc
     7141 gcagcggctg ccggggcagt actttgacga agaaacaggg ctgcattaca atctgttcag
     7201 gtattatgcg ccggagtgcg ggaggttcgt aagccaggac ccgataggat taaacggtgg
     7261 gttgaactta tatgcttatg cgcctaatcc gttaggttgg atcgatccgc taggactgaa
     7321 ttctttagga atagattcaa ataatatatt tagaggagat ggtaactata aaggtggcgg
     7381 aattggacag ccaaagagtg gcacaatatc agcacaggac attattgatc atctctaaga
     7441 aaaaggtgat gggcgtttaa catcttttag tactaaaaaa acatctagtt ccaactctcg
     7501 tggtgcagat tttttctcaa aaaaacatgt cttcaaagtg gcagtttctg atctcgatga
     7561 tttagttaaa aataatcaga tcactgtttt aacacaagaa aaagtaaaag caatatttgg
     7621 tttagaatca agcaagaaaa ttagaaaaga acttggtaat attcttcaga atatggctag
     7681 gaataatgag gtgttagttc atgggcaaat tcttggaaat aaagttagcc gatgtaatta
     7741 gttagtggag aggaagttgt ggacgaaatt agaataagaa ttgctggtaa tacggctgcg
     7801 ccttgtttcc atgtgctaaa aaataaggga tataaagtaa caatggaaac tcatgcatta
     7861 tctgacgatt gtttagattg tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt
     7921 agtgcaacga gtccagaaga gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat
     7981 gactggagag ctaatttgaa tgagataaat acttatcaaa aagccataga agaatccccg
     8041 ttttttgata aaaacggcaa tgaaatagag tctgactaaa ttttattgat atagcaacga
     8101 taataaccgg aaatttcttc cagatctttc cggcttttta ttatttaact ttcgttcagc
     8161 acactaaaca tcccgtctcg catcccctta actggctgtt taacctgata aagttccctg
     8221 cgcagttcct gtacttcgtt gttatcccaa gcaggatcaa acatccctcc gagatcttca
     8281 cggtaacgcc cgtgccgatc tcaaatcccg cttttctcag ccatttggcg ctgattttga
     8341 tcaccggcgt gtcgctgcgg ttgggcagat aacccacggt acagcgacgc tgtgttttgg
     8401 aaatggtgac ttctggcgta atattgtgtt taagttaggg gctttcgtaa tctagcccag
     8461 cccccggccc taaacacaac ccccactcac cacaacctaa actcatccgc atcccgccat
     8521 gccggaaact tttctctata ttcccgcaat gccaccatcg acagctccgc atcaatacgc
     8581 gttgccttat gcgcgtcggc ggtagcgata atctcgcctt gcggattaat cacccggctg
     8641 tcaccgcgat aatggcagcc gttgccatcg ctgccgacgc gattgcatcc cgccacatac
     8701 gcctgattct caatcgcgcg ggccgtcagc aatgcctgcc agtgcagaga gcgcggagct
     8761 ggccagttgg cgacgtacag ggcgaggtca taatcgttga gattgcgcga ccacactgga
     8821 aaacgtaagt cgtagcacac cagcggcaaa atacgccagc cgcgccattc cacaatcact
     8881 cgcgcattgc ccgctttata atgcagatgc tcatctgcca tgcggaacag atgacgctta
     8941 tcataaaaat gtaccgtgcc gcccggctca accagcaaaa agcggttaac cgaaccagac
     9001 tccgtttgta atgcaacact gcctgcaatc agcgcattgc actgctgcgc cttagctgtc
     9061 atccagttca ctacgtcatc ttgtgcaagc gacgaagctg ccgcttccat ggcaaagccg
     9121 ctggtaaaca tctccggtag aacgatcaca tcgcgcccgg taataccttc cagttgacga
     9181 tcaaaatggc gcaggttggc aggaccatcc acccacacca acggttgctg caaaagcgta
     9241 atcttcaaac taggcacggt gtacaactcc tttatgcgaa gggttttata actttaacac
     9301 cttatcaggc agttgcctta gcgcagaata aattgataac aaatgctgat attggaaata
     9361 tctgatttgc aaattatcgt gttatcgcta ggctttagga ggttaataac atgggcagga
     9421 taagctcggg aggaatgatg tttaaggcaa taacgacagt cgccgcactg gtcatcgcca
     9481 ccagtgcaat ggcgcaggat gatttaacca ttagcagcct tgcaaagggc gaaaccacca
     9541 aagctgcgtt taatcagatg gtgcaagggc ataagctacc tgcatgggtg atgaaaggcg
     9601 gtacttatac tcccgcacaa accgtaacgt tgggagatga gacgtatcag gtgatgagcg
     9661 cgtgcaaacc gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta
     9721 atcagatgac ggggctgttc tcggctattg atgagaaaac gtcgcaagag aaactcacct
     9781 ggctgaatgt gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc gcggcgttga
     9841 ccggcagcct ggaaaaccat ccggatggct ttaattttaa ataattagcg gataaagaaa
     9901 cggagccttt cggctccgtt attcaattac gcggcttcaa ctttccgcac tttctccggc
     9961 aactttaccg gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt aatactgcgc
    10021 agacggcttt cttccgcttt caccagaata gcggcttcat ctttatcaat cagccccttc
    10081 gtcagcgcgt tgtgcgccag ttcatccaga cgggtaaatg gcagattttt acccagctct
    10141 ttacagatcc gctgatgaat cgggtcggcg gcaatcacat ccaccaacgc ctcttccagt
    10201 aaaccaaccg ggttatgctc gcttggcgtc aggtactgac cgcgaccaat acgtgaacga
    10261 gtggcgttcg gcacttgtaa aatcttcgcc attttatgat ccagcttgtc agacggtgcc
    10321 agataatgac gtccggtcgg gaagatcacc acattcagca gcccggcaac cacgcggttc
    10381 gggaagtttt gcagtaaatt gtccattgcc tgttctgcct gatacagcgc atcctgtacg
    10441 ccccagtgca ccagcggcag atcggcttca ttacggcctt cgtcgtcata acgcttcaga
    10501 acggcagagg cgaggtaaag ctggcttaaa atatccccca gacgtgccga gatacgctca
    10561 cgacgtttca ggctgccgcc cagcagtgcc atcgagacat cagaaagcag ggcgaggttg
    10621 gcgctcaggc ggttcaggtg ctgatagtag cgtttagtgg catcgccggt tggcgtgctg
    10681 ctggttaaac cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct accgacgtga
    10741 ccgatatgtt tgaacaacag tttatcgaac gcgttgacgt cattgttctt cgccgcttcc
    10801 atctcttcca gcacgtacgg atggcaacga atcgctcctt gtccgaagat catcatgctg
    10861 cgggtcagaa tgttagcccc ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc
    10921 gccaggaagt tgctttgccc gagcataatg cctttaccgc cggtaatatc catcgcatca
    10981 ataatcgact gctgcccgcg gtgggtacag tgatacttaa cgatagccga cagcacggca
    11041 ggtttttcgc cgagcataat gccgtaggta atcagcgatg ccgcagcatc cattacataa
    11101 gcgttcccgg caatacgcgc cagcggctct tcaatccctt ccatcttacc gatagagatt
    11161 ttgaactgac ggcgaatgtg agaatacgcg cccgttgcca gcgctaccga tttcacgccg
    11221 ccggttgagt tggaaggcag ggtgatgccg cggcctaccg agaggcactc caccagcatc
    11281 cgccagcctt gcccggccat tttcggcccg ccgatgatgt aatcgatcgg cacgaagaca
    11341 tctttaccgc gcgtcggtcc gttctggaac ggtacgttca gcgggaagtg gcgacgacca
    11401 atttccacgc ccggcgtggt ggttgggatc agcgcacagg taatgcctaa atcttctgca
    11461 ccgccgagta atttttccgg gtcggagagt ttaaacgcca gcccaagcac ggtcgcaatc
    11521 ggtgccagcg taatgtagcg tttgttccag gtcagacgca tccccagcac ctgctggccc
    11581 tgccattcgc ccatgcagac aatcccggtg tccggaatcg cgcccgcatc ggaacccgct
    11641 tccgggctgg tcagtgcaaa gcaggggatc tcctgaccac gcgccagacg cggcagatag
    11701 tgatctttct gctcgtcagt gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt
    11761 ggcacgccga cggtaatcgc caggatcccg ctcacgccag agagtttttg cagcacacga
    11821 gactgggcat aagctgagaa ctccagtccg ccgtactctt ttttgataat catcgcgaag
    11881 aagcgatgct ctttaaggta cgcccacaac tccggtggca gatccgccag ctcatgagta
    11941 atctggaaat cattcgccat ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc
    12001 tcttcagcgg tcaggcgcgg ctgcggatag ttatgcagct ttttccagtc cggcttgccc
    12061 tggaacaagt cgccctccca ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc
    12121 gacatcggcg gcatcacctt acggaaaccg cgaaataccg gcgcggaaat catcgactta
    12181 cgcataggcg caaagttaaa tggcacgagg ataatggtca gaggcaccag tacccacgcc
    12241 gaccacagac cagcaacgcc gagggcggct gtccaggcga gcaaaatcag actgctgata
    12301 aataagctca cgcggtgata gaacaacgcg ccgagcagga caaccgtagc gagaatactc
    12361 aaaatcatca taacgaaaag ccccttactt gtaggaggtc tgaccacttg tgatgatatg
    12421 gttgtagtgg atgtaaaaac atttagcaat gtgtttacaa tataattaca acaaagctca
    12481 cattgttgct gtttttatcc gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg
    12541 tacttctcgc ttttggcggt atccggtaca ctgcattttg tctattacat ttatgctgaa
    12601 ggatatcctc atgtaccagg atcttattcg taacgaactg aacgaagcgg cggaaacgct
    12661 ggctaacttt ttaaaagatg acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt
    12721 agcagacagc tttaaagccg gtggcaaagt gctttcctgc ggcaacggcg gttcccattg
    12781 cgacgctatg cactttgccg aagagttgac cggtcgctac cgtgaaaacc gtccgggcta
    12841 cccggcgatt gctatttctg acgttagtca tatttcctgc gtcggtaatg atttcggttt
    12901 caatgatatt ttctcccgct acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg
    12961 gatctccacc tccggtaact ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa
    13021 ggggatgaaa gtgatcaccc tgaccggtaa agacggcggt aaaatggctg gcacggcgga
    13081 tatcgaaatt cgcgttccgc actttggtta tgccgaccgc attcaggaga ttcacattaa
    13141 agtgatccat atcctgatcc agttaattga aaaagagatg gttaagtaag tctggcgtag
    13201 gccggataag gcgtttacgc cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg
    13261 acgcgtctta tcatgcctac aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg
    13321 ccgcatccga cctgaaaatt cttaaatcaa tcttcgccgg ggtccatgcg ctcccgctgt
    13381 tgtggaggtt acccatgtgc gaattgctcg ggatgagcgc caacgtccct accgatatct
    13441 gctttagttt caccggactt gtacagcgtg gtggtggaac cgggccacat aaagatggct
    13501 ggggcattac cttttacgaa ggtaaaggct gtcgcacatt taaagatcca caacccagct
    13561 ttaattcccc catcgccaaa cttgtccagg actacccgat aaaatcctgt tcggtggtgg
    13621 ctcatattcg ccaggctaat cggggcgagg tggcgctgga aaatactcac ccatttaccc
    13681 gcgagttatg ggggcgtaac tggacttatg cccataacgg acaactgacg ggctacaaat
    13741 cactggaaac cggcaacttc cgcccggtcg gtgaaaccga cagcgaaaaa gccttttgct
    13801 ggctgctgca taaattaacg cagcgttacc cgcgcacgcc gggcaacatg gcggcagtgt
    13861 ttaaatatat cgcctcactg gcggatgaac tgcgggcgaa gggcgttttc aacatgctgc
    13921 tttcggacgg gcgctatgta atggcgtatt gctcgactaa tttacactgg atcacccgcc
    13981 gcgcgccgtt tggcgtggca acgttgctgg atcaggatgt ggaaatcgac ttcagctcgc
    14041 agaccacacc gaatgatgtg gtcacggtga ttgcgacaca gccgctgacg ggcaatgaaa
    14101 cctggcaaaa aattatgcca ggcgaatggc gcttattttg cctcggggag cgtgtagttt
    14161 gatgccagtt gtggctgcac cacttcgtgg cttaacggct tgctgaccac gtaacggcca
    14221 ttggccacag aaacggtcgg tggcttacgg gtttgctcaa agtagtcgta gcccggcttc
    14281 agctgctccc agaagtcctt aaagttggaa tatttatggc gcttcatatt ggcgtcggtc
    14341 atgcggaatg ggtaaatact cacctgcacg ctcggctgac caaacaccag cgcaccagta
    14401 acgaactgga agatctcatc aataccctga ttggtcattg cgtagcagcc gatggataca
    14461 caatcgccgt ggatcatcag gtatttccct tcataaccat gcgcacggtc ataagcattg
    14521 gggaatccaa tattaatcgc tttgtagtaa cggctgtctg gttttaactg attacgctgg
    14581 acgctataaa acccttccgg gcttttgaaa tcgccctgac gctgttttgg ccctaagccg
    14641 ccggaatatt tacagatttt ataactgtcg agcagttgat attgctcgcc cattttgacg
    14701 taaagatcga gcgtacgttc ttccttgaag atctggatgt agacagggga tcccatcaac
    14761 tgctgcttat actctttgct cactggcgtg gtcgagctac tgctgcccag caaaccggca
    14821 aacgaaacgc acgggatcaa caacatcgca agaattaatg cgattttacg catactgctt
    14881 attccttgat aaaacggtta cacacgccag gacggcaaaa tggatcccaa atcggaatag
    14941 tctggatttg gaaggctcac attatcacca aaagagtttt acgcaagcct gtcggcgctg
    15001 ggtttacaaa tttagcacag gggcagtttt caatagtccg aaaagcgcat gtatcattta
    15061 tttcctaata ttatgaaatc tcaatagtaa tatgcaaata agcaattaat aatcccgttt
    15121 actcctccgg tcttttaaaa ttctcacctt cattcagccg gtttatgtct ttcatgtctt
    15181 cgttcttgct cggcagattt ttgcatccag gcgtgttttc gttatgcgtt ttgctccctt
    15241 tactcgccag cgccaccaca accgggagca gataaaacag catccagaat ggtttcctgc
    15301 gccgctcaag gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc actttttaag
    15361 cagcgatcac ctgcataaca tcaccgaagt ggcgattcac cgcctggagc agcagcttgg
    15421 caagccttac gtctggggcg gcacgcggcc cgatcaaggc ttcgattgta gtgggttagt
    15481 tttttatgcc tacaacaaga tccttgaggc gaaactcccg cgcacggcca atgagatgta
    15541 ccactaccgt cgggcaacga ttgtggcgaa taacgacctg cgccggggag atttgctatt
    15601 tttccatatc cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca
    15661 gtttattgag tcactgcgca ccggggaaac cattcgggta agccggttag ccgaaccttt
    15721 ctggcaggac cattttttag gcgcgcgcag gattttgacg gaagagatga ttttgtaggc
    15781 cggataaggc gtttacgccg catccggcgg ttgtgcacgg atgcctgatg cgacgctgac
    15841 gcgtcttatc atgcctacaa atctgtgcgt ttttgcgagc cgctttcccg atataacaca
    15901 gcgtcattgt cggcctttcc ttttcctctt accgttgaga aaaaggagtc gctatgtcgg
    15961 aatatcgtcg ttattacatc aaggggggaa catggtttgt cacggtaaat ttacgcaatc
    16021 gtcgaagcca tcttctgacc acccagttcc agacgctccg taacgccatt attaacgtta
    16081 agcgagacag accttttgaa attaacgcct gggtcgtttt gccggagcat atgcattgca
    16141 tatggacatt acccgaaagc gatgatgatt tttcctctcg ctggcgggag attaaaaagc
    16201 agttcaccca tgcttgcgga ttgaaaaata tctggcaacc gcgtttttgg gagcatgcca
    16261 ttcgcaatac aaaagattat cgccatcatg ttgattatat ttatataaat ccagagaaac
    16321 atggctgggt aaagcaggtg agtgattggc cattctcaac gttccatcgc gatgtcgcgc
    16381 gagggttata tcccatcgat tgggcgggag acataacgga tttaagtgcc ggggagcgta
    16441 tcattttata actgtgcgct ggtgccggat gcgcttacgc ttatccggcc tacaaatcgt
    16501 gctttaacgg taggccggat aaggcgttca cgccgcatcc ggcagttatg cgcagatgcc
    16561 tgatgcgacg ctggcgcgtc ttatcatgcc taaaaacttg tgcctgaacg gtaggccgga
    16621 taaggcgttt acgccgcatc cggcaattgt gcaccgatgc cggattctta catcaacgcc
    16681 cccataatct tcaactccag ctcatccggc acttcgttat acgacagcac atgcagcccc
    16741 ggcgcaaaca accttgcata acgcggcagc aaagggcgta gctgcggcgg caccagcagc
    16801 accggatctt tccccgccgc tttcatctgc tctttcacct gtggcatcgt gctctggaac
    16861 tggttgagca tattcgggtc aaccggcacg ctgtcgagca tcactttgcc ggcctgttgc
    16921 gcctgattca ctacgttggt cagcagattt tccagctcat tattcagcgt atacaccgtc
    16981 agctcctgct tgcgaacgaa cggatgagta atgctgcgcc gcagcgccag gcgcacatcg
    17041 gccgccagca gaatatgatc tttagtcacc gtgctactgg cgaccagcac ggtggcgatg
    17101 gtgacgatat cgcgcaggga aacgccttcg gtcagcagcg cccggtacac tttcagcaat
    17161 tggctgtaat tgagcgccgc gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa
    17221 cggttatgca actgcgtgat gtcatcatag ttaaacaaat caggaatata gctgcgcaca
    17281 atcttgttca catgggtggc aatcacactg gcgctgtcga tcacctgata ccccatattc
    17341 agagccttcg ctttctgcgc aggctggatc caggtgaccg gtatcccgta cgccgggtcg
    17401 ttccccagca cgccgtcaat ctcgccgtag gtttcgctgg agggcagcgc catcagttta
    17461 tccgccggaa tatccgcttc atcagcctca atgccgttga tgaaaatagc gtactggctg
    17521 ggcttgaggc ggaagttttc ccgaatgcgg atctccggca gcagcacgcc gttaccgtcg
    17581 gaaatcacct gccgaacgcc gcgaatccgc tgggtgagcg ggttgccctg cgctttgtcc
    17641 accagcgcca ccagcttgta gccgaggctt aagctgatgg gctcgatcag cggaatagtt
    17701 tcccaactga cctgttgttc gctggtttca gtgatggtgc gggtcagcgt ttcgaggctt
    17761 ttctcttccg cctccgccgc ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc
    17821 agggcgctga acagcaaaaa cggcagatgc ggcatccccg gcaccaccgc cagcacgaac
    17881 atgatcccgg tggcggtgta gagcaccgac gggcttgcca gcaactggtg gcgcacgtca
    17941 tgggcgatat cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc ggtggagagc
    18001 agcagggaag ggatctgcgc caccaggccg tcgccgatgg tcatcagcac gtattgctgg
    18061 aaggcggcgt cggcgctcag gttgtatttg aagatgccga tacagacgcc gccgctcagg
    18121 ttgatcgcca gaatcatcat cccggcgatg gcgtctccgc gtacaaactt cgatgccccg
    18181 tccatcgcgc cgtagaaatc ggcctcgctg gcaacatctt tacgccgggt ttgcgcctgc
    18241 gcctggttga tcaatccggc gttaagatcg gcgtcaatcg ccatctgttt gccgggcatc
    18301 gcgtccaggg taaagcgggc agaaacctcg gaaatacgct cggcctcttt ggtgacgacg
    18361 ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg
    18421 cacgggcagt cgatttttaa cggtggtggg ctgtcgccgt taaataaaat cagcccgtct
    18481 catccgccaa aacctgccac cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgat
    18541 gtgaatgaga aaacggcgct gctgaagaag aaatccgcca ccgagcttgg cgagttggca
    18601 accagtatca acaccattgc ccgtgatgcg catatggaag cgaatctgga gatggagatt
    18661 gttccccagg gattacgcgt gctgattaaa gacgaccaga atcgcaatat gttcgaacgc
    18721 ggcagcgcgc agattatgcc gttctttaaa actttgctgg tggagctcgc gccagtgttc
    18781 gactcgctct ataacaaaat tattattacc gggcataccg atgcgatggc ctacaaaaac
    18841 aatatctaca acaactggaa cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg
    18901 gaagaggccg gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg
    18961 ctgctggacg cgaagaatcc gcaaagcgcg ggcaatcggc gtattgagat tatggtgctg
    19021 accaaaagtg cgtccgatac gctgtatcaa tactttggtc agcatgggga taaagtggtg
    19081 cagccgctgg tgcaaaagct ggataagcag caggtgcttt cgcagcgaat gcgctaaatg
    19141 ctgaatcttt acgcatttct caaaacctga aatcactgta tactttacca gtgttgagag
    19201 gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgcag cggtggagat
    19261 gcgcgacaat cccgctctgc gcgatatccc tattgctatt ggcggcagcc gcgaatgtcg
    19321 gggggtgatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc
    19381 tactgggatg gcgctcaaat tatgcccaca tctcaccttg cttccggggc gctttgacgc
    19441 ctacaaagaa gcctcaaatc atatccgtga aatcttctcg cgctacacct cgcgcattga
    19501 accgttgtca ctggatgagg cttatctcga tgtcaccgat agcgtccatt gccacggttc
    19561 tgcgaccctc atcgcccagg aaatccgcca gacgatcttc aacgagctgc aactgacggc
    19621 atctgcgggc gtggcaccag taaagtttct cgccaaaatc gcctccgaca tgaataaacc
    19681 caacggtcag tttgtgatta cgccggcaga agttccggca tttttacaaa ccttaccact
    19741 ggaaaaaatc cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg
    19801 gacctgcggt gatgtacaaa agtgtgatct ggtgattctg cttaaacgct ttggcaaatt
    19861 tggccgcatt ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta acagcgaacg
    19921 gttacgaaaa tccgtcggcg tggaacgcac gatggcggaa gatattcatc actggtctga
    19981 atgtgaagcg attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa
    20041 acctgattta ctgattgctc gccagggggt gaaattaaag ttcgacgatt ttcagcagac
    20101 cacccaggag cacgtctggc cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa
    20161 aacctgggat gaacgccgcg gcgggcgcgg tgtgcgtctg gtggggctgc atgtgacgtt
    20221 gcttgacccg caaatggaaa gacaactggt gctgggatta tgatgtatac tattatgtat
    20281 attctggtgt gcattattat gagggtatca ctgtatgcat cgaattctcg ctgaaaaatc
    20341 ggtcaatatc actgagttac gtaaaaaccc agctaaatac tttattgatc aaccagttgc
    20401 ggttctttct aataatcgcc ccgcaggata tctcttaagt gccagcgcat tcgaagcgtt
    20461 aatggacatg cttgctgaac aagaggagaa aaagcccata aaggcgcgct ttcgtccaag
    20521 tgctgcaaga ttagaggaaa ttacacgccg cgctaaacaa tatcttaatg atatgacgga
    20581 tgatgatttc aatgacttta aggaataagg atgcgggtat tcaaaacaaa acttattcgc
    20641 ctgcaactta cagcagagga acttgatgcg ttaacggcgg attttatttc ctataagcgt
    20701 gacggtgttt tgccagatat atttggtcgc gatgcactct acgacgactc gtttacctgg
    20761 ccattaatca aatttgagcg agttgctcat attcatctgg caaatgtgaa taatccattt
    20821 ccgccacagt tgcgccaatt cagcagaacg aatgacgaag cgcatttggt atattgtcag
    20881 ggtgcgtttg atgagcaagc atggttgctc attgccattc tgaagcctga acctcataaa
    20941 ctggctcgag ataacaacca aatgcataaa attggaaaaa tggcagaagc gtttcgcatg
    21001 cgtttttgaa tttatattat gaataacata caaataagag attatcagcc tggcgatttt
    21061 cagcaactat gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca
    21121 ctacaacaaa ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg
    21181 aaatcacaag tgcgggttgc agttattaat gcaaaactgg tcggctttat tacctgcgtt
    21241 gaacactata tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc
    21301 gctttgttaa aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc
    21361 gcaaaaccct tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc
    21421 cggggagcgt ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt
    21481 gcaatgaaaa taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga
    21541 ggtaaatcat gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct
    21601 ttttttcttg ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa
    21661 ttcaagtcgc agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg
    21721 gcaattatat tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga
    21781 tcgagagttt agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg
    21841 tagtggggat gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct
    21901 ccgcaggccg tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat
    21961 ggcaaacaga tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag
    22021 ctttggatga cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac
    22081 agcatccgtg gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc
    22141 aacaagttga tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc
    22201 atctgcaact gctggttcat agcggctcga gggggctagg gcagtctatt ttacagcggc
    22261 atattgcctc gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg
    22321 cgcgttttaa tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa
    22381 gtccggttct ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag
    22441 gggtcttaca tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt
    22501 cacgcggtga ttactccagg ctggttcagc ccgtcaggag tgaggaaaca ttgcattcgc
    22561 tggcgcatgg ggctgggcgt aaatgggggc gcaccgagtg taaagggcgt ctggcagcga
    22621 aatacacagc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata
    22681 aacaactcat cttcgaagaa gcgccacaag cctataaatc ggctgaaagt gtggtgcaat
    22741 gtcagggcag gccggattaa ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa
    22801 aaacagtgga gggaaaaaag gatgatcttg ctataactct cctcagcgca ggggccggaa
    22861 gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa
    22921 gacgtcgcgg taacggtgct ggaaacagaa acaggccgct actctgacac gctgcgttcg
    22981 gcgctgattt ctctggatgg cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact
    23041 attcagtgga tttgcccgag cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg
    23101 ggcattgggc gttttaccgc tgatgagcag gagcaatccg atgcgatccg gtatgagacg
    23161 ctgcgttcgt cggggccggg cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc
    23221 acgcatttgg catccggtat tagcgtgaag gttcagtcag agcgcagcca gcatgctaac
    23281 aaacggctgg cgcgattgct gattgcctgg aagctggatc agcagcaata ggaaaatagc
    23341 gcggtgctga aatcacagcg gcgaatgttc catcaccaga ttgaacgtgg caacccgcga
    23401 cgaacgttta cagggatggc ttttatcgaa ggataatgaa ggatgaaact gccggaaggc
    23461 gattaaacgc catccggcag caaatcaaat aattacttcg ccggaatttc tttcagcagt
    23521 tcagtcagca gtgtccagta atgacctacg ctttcgatgt gaacttgctc atccggagag
    23581 tgtggaccgg tgatagttgg cccgatagaa accatgtcca tttccggata cggttttttg
    23641 aacagaccac attccaggcc cgcgtggata atctggatgt tcggcgtctt gttgaacagg
    23701 cgctgatagg tttcacgtac cagatgcatc accggagaat tagcgtccgg ctgccagcca
    23761 ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt tacccagcga atccagcatg
    23821 ctcaccacgt agtctttacc gctgtcgatt agtgaacgga tcaggcagtg aatttctacg
    23881 ttattgtcag tcatggtcac cacaccgacg ttcagggagg tttcgaccac accttttgcc
    23941 acgtcggaat tgcggatcac accgttcggg gtggcgttca gcagacgaat aaaggtatcg
    24001 cgagatttcg caatcagggc agctttatcg ttcgctacag agtccagcaa caaggccaga
    24061 tttttctctt tctctgccag ctcgtttttc aggatctcct gataggtatt caccagagat
    24121 ttcagtgcgt cgactttatc agctgcgaca gcaatggtcg caaaggcttc acgcgggatg
    24181 gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca ggtccagttc ttctgcatga
    24241 cccgccagga agcgcaccag cagtttgttg gcattaccca ggccaacgtg gatttcaccg
    24301 ccggagtgac cgcctttcag accttttaag gttaacttga aggtttcaaa accagctgga
    24361 accgcttcac gatctaaatg caggttggag gtgaagtcga taccccccgc acaacccatg
    24421 tagatttcac cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg caaccagttg
    24481 ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg tcatggtcag cagcacttcc
    24541 agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg
    24601 ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc attcgccatc aatataaggc
    24661 tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg
    24721 aggtgggcct gtaagacgac cggtttacga ttttccatac ctgcggtagc aggttgacga
    24781 atcaggatat tacctacctg atcgcgttcg acatggaaac ctttctcttt tgcccaacca
    24841 acaatgtatt cagcgagttg ctcttcatga taggacgggt gaggaataga acagattttg
    24901 gcaaaaatat cccacagcgg ctgtggagat aattgagaca gttcagacac gttaagtctc
    24961 cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa
    25021 cacaagacag gcttgcgaga tatgtattga gaataccact ttatcccgcg tcagggagag
    25081 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    25141 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    25201 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    25261 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    25321 gtggcggtct ggtaccgggg gcgttactgg cgcgtgaact gggtattcgt catgtcgata
    25381 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
    25441 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
    25501 ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
    25561 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggatcgaac
    25621 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
    25681 gcctggcatt gccgggcgtt gttcttttta acttcaggca ggttacaata gtttccagta
    25741 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
    25801 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
    25861 cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
    25921 ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt
    25981 gtgatgagcg atgctgaacg taccgacgat gatttatacg ataccgtgat tggctaccgt
    26041 ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt
    26101 acggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac gttgtacaac
    26161 attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttatca
    26221 aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca gatggagttt
    26281 accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg
    26341 ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg
    26401 tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg
    26461 ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta
    26521 aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt
    26581 ttcggcgcta acgttgccgt gcgtctggca taccttgaat cgccgcgtct gaaagcggtt
    26641 gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg
    26701 ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa
    26761 gcgttacgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc
    26821 tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac
    26881 tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc atttaacccg
    26941 gtgtatcgga attttgacaa aggtcttcag gaaatcaccg gctggatcga aaaacgcttg
    27001 tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca acaggagata
    27061 gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgcacta
    27121 ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct
    27181 gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgaat tctggggctg gtggatggag
    27241 ctggaagcgc aggaatcccg ttttacctac agctaccagt ttggtctgtt cgataaagca
    27301 ggcgactgga agagtgttcc ggtaaaagat actgaagtgg ttgaacgact ggagcacacc
    27361 ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa
    27421 ccggcgaatg attttcgtga tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga
    27481 tgcatcacat ccggcaatat tcattaaaac tgatacgtca tcccaaccgc gacagtatca
    27541 tcattattaa tattcagctt attatcgcta tccagttggt tgattttata atcaacaaac
    27601 gctgacatat ttttgttgaa ataatacgta gcaccgacgt cgatataatt gaccagatct
    27661 tcatcaccga tgccctcaat atctttccct ttcgataaga cataacccag cgatggacgc
    27721 agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag
    27781 ccgccagtta ttggcgtcat tttgcgtgtt tcagaataga aagttgccag ataaatatta
    27841 ttggcatcgt atttcagacc tgttgcccat gcttctgcac gcttgcctgt gccacggctt
    27901 tgcaggttct gctcgttggt gcgatctgag ttggtatagg ccccactaat ggcgaaatcg
    27961 ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg ctttttaacg
    28021 tcgcggtttt cgtttttccc ttgatattgc agggttaagt tcaggccatc gataacgccg
    28081 aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg
    28141 gtctgcgccg aggaatcgcc accaaattcc gggaacatat cggtccaggc ttccacgtca
    28201 tacaacgcgc ccaggttacg accataatcg aaagaaccca aatctttata tttcaacccg
    28261 gcaaaagcga gacgcgtttt ttgctgtgca gtatcactct ccgctttatt tccggcaaac
    28321 tccgcttccc accgaccata accagtcagt tgatcgttaa tttgagtttc acctttaaaa
    28381 ccaaaacgga tataactctg gtcgccatct ttactggcgt tatcactcat ataatgcatg
    28441 gctttaactt tgccatagac atccagttta ttaccgtctt tattatatat ttctgcagcc
    28501 tgtacagatg cagatgccac aatgcccatc accactaatg ccagagtgct ctttttcatt
    28561 ttcattcctg attttaatta acgcgcgaat attcagcggg agagccccgt tgaaaacagg
    28621 aaagttttta acctgagatt gttaaagata tattacagat taatgatatt
//
LOCUS       DAZOZX010000041        28223 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZOZX010000041 DAZOZX010000000
VERSION     DAZOZX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.041"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUPT_001741"
     CDS             complement(<1..209)
                     /locus_tag="ACHUPT_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0819342.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUPT_001742"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUPT_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0819343.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUPT_001743"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUPT_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0819344.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUPT_001744"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUPT_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0819345.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUPT_001745"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUPT_001745"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0819346.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUPT_001746"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUPT_001746"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0819347.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUPT_001747"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUPT_001747"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0819348.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHUPT_001748"
     CDS             complement(4598..5320)
                     /locus_tag="ACHUPT_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0819349.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUPT_001749"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUPT_001749"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0819350.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHUPT_001750"
     CDS             6520..6738
                     /locus_tag="ACHUPT_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0819351.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUPT_001751"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUPT_001751"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0819352.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHUPT_001752"
     CDS             complement(7472..7672)
                     /locus_tag="ACHUPT_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0819353.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUPT_001753"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUPT_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUPT_001754"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUPT_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0819354.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACHUPT_001755"
     CDS             10101..12014
                     /locus_tag="ACHUPT_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0819355.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACHUPT_001756"
     CDS             12014..13036
                     /locus_tag="ACHUPT_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0819356.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACHUPT_001757"
     CDS             13030..13248
                     /locus_tag="ACHUPT_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0819357.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACHUPT_001758"
     CDS             13302..14171
                     /locus_tag="ACHUPT_001758"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0819358.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACHUPT_001759"
     CDS             complement(14226..14630)
                     /locus_tag="ACHUPT_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0819359.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACHUPT_001760"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACHUPT_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0819360.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACHUPT_001761"
     CDS             15615..17705
                     /locus_tag="ACHUPT_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0819361.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHUPT_001762"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHUPT_001762"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0819362.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHUPT_001763"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHUPT_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0819363.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACHUPT_001764"
     CDS             complement(19673..20275)
                     /locus_tag="ACHUPT_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0819364.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACHUPT_001765"
     CDS             complement(20265..20432)
                     /locus_tag="ACHUPT_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0819365.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHUPT_001766"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHUPT_001766"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0819366.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHUPT_001767"
                     /pseudo
     CDS             21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHUPT_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="ACHUPT_001768"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="ACHUPT_001768"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0819367.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="ACHUPT_001769"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="ACHUPT_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0819368.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="ACHUPT_001770"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="ACHUPT_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0819369.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cysG"
                     /locus_tag="ACHUPT_001771"
     CDS             26648..28021
                     /gene="cysG"
                     /locus_tag="ACHUPT_001771"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0819370.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6853 a         7543 c         7105 g         6722 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgctaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28081 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28141 tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28201 cgagcagtct gcttatattc ata
//
LOCUS       DAZOZX010000042        28183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZOZX010000042 DAZOZX010000000
VERSION     DAZOZX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.042"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHUPT_001772"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHUPT_001772"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0819371.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUPT_001773"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUPT_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0819372.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUPT_001774"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUPT_001774"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0819373.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUPT_001775"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUPT_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0819374.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUPT_001776"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUPT_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0819375.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUPT_001777"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUPT_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0819376.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUPT_001778"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUPT_001778"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0819377.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9168
                     /gene="atpI"
                     /locus_tag="ACHUPT_001779"
                     /pseudo
     CDS             8747..9168
                     /gene="atpI"
                     /locus_tag="ACHUPT_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
     gene            9177..9992
                     /gene="atpB"
                     /locus_tag="ACHUPT_001780"
     CDS             9177..9992
                     /gene="atpB"
                     /locus_tag="ACHUPT_001780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0819378.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            10039..10278
                     /gene="atpE"
                     /locus_tag="ACHUPT_001781"
     CDS             10039..10278
                     /gene="atpE"
                     /locus_tag="ACHUPT_001781"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0819379.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10340..10810
                     /gene="atpF"
                     /locus_tag="ACHUPT_001782"
     CDS             10340..10810
                     /gene="atpF"
                     /locus_tag="ACHUPT_001782"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0819380.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10825..11358
                     /gene="atpH"
                     /locus_tag="ACHUPT_001783"
     CDS             10825..11358
                     /gene="atpH"
                     /locus_tag="ACHUPT_001783"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0819381.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11371..12912
                     /gene="atpA"
                     /locus_tag="ACHUPT_001784"
     CDS             11371..12912
                     /gene="atpA"
                     /locus_tag="ACHUPT_001784"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0819382.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12963..13826
                     /gene="atpG"
                     /locus_tag="ACHUPT_001785"
     CDS             12963..13826
                     /gene="atpG"
                     /locus_tag="ACHUPT_001785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0819383.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13853..15235
                     /gene="atpD"
                     /locus_tag="ACHUPT_001786"
     CDS             13853..15235
                     /gene="atpD"
                     /locus_tag="ACHUPT_001786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0819384.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15256..15675
                     /locus_tag="ACHUPT_001787"
     CDS             15256..15675
                     /locus_tag="ACHUPT_001787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0819385.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            16026..17396
                     /gene="glmU"
                     /locus_tag="ACHUPT_001788"
     CDS             16026..17396
                     /gene="glmU"
                     /locus_tag="ACHUPT_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0819386.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17558..19387
                     /gene="glmS"
                     /locus_tag="ACHUPT_001789"
     CDS             17558..19387
                     /gene="glmS"
                     /locus_tag="ACHUPT_001789"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0819387.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19549..20370
                     /locus_tag="ACHUPT_001790"
     CDS             19549..20370
                     /locus_tag="ACHUPT_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0819388.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20357..22465
                     /locus_tag="ACHUPT_001791"
     CDS             20357..22465
                     /locus_tag="ACHUPT_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0819389.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22462..24129
                     /locus_tag="ACHUPT_001792"
     CDS             22462..24129
                     /locus_tag="ACHUPT_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0819390.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24132..25658
                     /locus_tag="ACHUPT_001793"
     CDS             24132..25658
                     /locus_tag="ACHUPT_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0819391.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25659..27275
                     /locus_tag="ACHUPT_001794"
     CDS             25659..27275
                     /locus_tag="ACHUPT_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0819392.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27331..27483)
                     /locus_tag="ACHUPT_001795"
                     /pseudo
     CDS             complement(27331..27483)
                     /locus_tag="ACHUPT_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(27506..27883)
                     /locus_tag="ACHUPT_001796"
     CDS             complement(27506..27883)
                     /locus_tag="ACHUPT_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819393.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         7297 a         6548 c         7273 g         7065 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcagtctt tctgcctaac
     8941 gttttgttta tgatatttgc ctggcgtcac caggcgcata caccagcgaa aggccgggtg
     9001 gcttggacat tcgcatttgg cgaagctttc aaagttctgg cgatgttggt gttactggtg
     9061 gtggcgttgg cggttttaaa ggcggtattc ttgccgctga tcgttacgtg ggttttggtg
     9121 ctggtggttc agatactggc accggctgta attaacaaca aagggtaaaa ggcatcatgg
     9181 cttcagaaaa tatgacgccg caggattaca taggacacca cctgaataac cttcagctgg
     9241 acctgcgtac attctcgctg gtggatccac aaaacccccc agccaccttc tggacaatca
     9301 atattgactc catgttcttc tcggtggtgc tgggtctgtt gttcctggtt ttattccgta
     9361 gcgtagccaa aaaggcgacc agcggtgtgc caggtaagtt tcagaccgcg attgagctgg
     9421 tgatcggctt tgttaatggt agcgtgaaag acatgtacca tggcaaaagc aagctgattg
     9481 ctccgctggc cctgacgatc ttcgtctggg tattcctgat gaatctgatg gatttactgc
     9541 ctatcgactt gctgccgtac attgctgaac atgtactggg tctgcctgca ctgcgtgtgg
     9601 ttccgtctgc ggacgtgaac gtaacgctgt ctatggcact gggcgtattt atcctgattc
     9661 tgttctacaa catcaaaatg aaaggcatcg gcggcttcac gaaagagttg acgctgcagc
     9721 cgttcaatca ctgggcgttc attcctgtca acttaatcct tgaaggggta agcctgctgt
     9781 ccaaaccagt ttcactcggt ttgcgactgt tcggtaacat gtatgccggt gagctgattt
     9841 tcattctgat tgctggtctg ttgccgtggt ggtcacagtg gatcctgaat gtgccgtggg
     9901 ccattttcca catcctgatc attacgctgc aagccttcat cttcatggtt ctgacgatcg
     9961 tctatctgtc gatggcgtct gaagaacatt aatttaccaa cactactacg ttttaactga
    10021 aacaaactgg agactgtcat ggaaaacctg aatatggatc tgctgtacat ggctgccgct
    10081 gtgatgatgg gtctggcggc aatcggtgct gcgatcggta tcggcatcct cgggggtaaa
    10141 ttcttggaag gcgcagcgcg tcaacctgat ctgattcctc tgctgcgtac tcagttcttt
    10201 atcgttatgg gtctggtgga tgctatcccg atgatcgctg taggtctggg tctgtacgtg
    10261 atgttcgctg tcgcgtagta agcgttgctt ttatttaaag agcaatatca gaacgttaac
    10321 taaatagagg cattgtgctg tgaatcttaa cgcaacaatc ctcggccagg ccatcgcgtt
    10381 tgtcctgttc gttctgttct gcatgaagta cgtatggccg ccattaatgg cagccatcga
    10441 aaaacgtcaa aaagaaattg ctgacggcct tgcttccgca gaacgagcac ataaggacct
    10501 tgaccttgca aaggccagcg cgaccgacca gctgaaaaaa gcgaaagcgg aagcccaggt
    10561 aatcatcgag caggcgaaca aacgccgctc gcagattctg gacgaagcga aagctgaggc
    10621 agaacaggaa cgtactaaaa tcgtggccca ggcgcaggca gaaattgaag ccgagcgtaa
    10681 acgtgcccgt gaagagctgc gtaagcaagt tgctatcctg gctgttgctg gcgccgagaa
    10741 gatcatcgaa cgttccgtgg atgaagctgc taacagcgac atcgtggata aacttgtcgc
    10801 tgaactgtaa ggagggaggg gctgatgtct gaatttatta cggtagctcg cccctacgcc
    10861 aaagcagctt ttgactttgc cgtcgaacac caaagtgtag aacgctggca ggacatgctg
    10921 acgtttgccg ccgaggtaac caaaaacgaa caaatggcag agcttctctc tggcgcgctt
    10981 gcgccagaaa cgctcgccga gtcgtttatc gcagtttgtg gtgagcaact ggacgaaaac
    11041 ggtcagaacc tgattcgggt aatggctgaa aatggtcgtc ttaacgcgct cccggatgtt
    11101 ctggagcagt ttattcacct tcgtgccgtg agtgaggcta ccgctgaggt agacgtcatt
    11161 tccgctgccg cactgagtga acaacagctc gcgaaaattt ctgctgcgat ggaaaaacgt
    11221 ctgtcacgca aagttaagct gaattgcaaa atcgataagt ctgtaatggc tggcgttatc
    11281 atccgagcgg gtgatatggt cattgatggc agcgtacgcg gtcgtcttga gcgccttgca
    11341 gacgtcttgc agtcttaagg ggactggagc atgcaactga attccaccga aatcagcgaa
    11401 ctgatcaagc agcgcattgc tcagttcaat gttgtgagtg aagctcacaa cgaaggtact
    11461 attgtttctg taagtgacgg tgttatccgc attcacggcc tggccgattg tatgcagggt
    11521 gaaatgatct ccctgccggg taaccgttac gctatcgcac tgaacctcga gcgcgactct
    11581 gtaggtgcgg ttgttatggg tccgtacgct gaccttgccg aaggcatgaa agttaagtgt
    11641 actggccgta tcctggaagt tccggttggc cgtggcctgc tgggccgtgt ggttaacact
    11701 ctgggtgcac caatcgacgg taaaggtccg ctggatcacg acggcttctc tgctgtagaa
    11761 gcaatcgctc cgggcgttat cgaacgtcag tccgtagatc agccggtaca gaccggttat
    11821 aaagccgttg actccatgat cccaatcggt cgtggtcagc gtgaattgat catcggtgac
    11881 cgtcagacag gtaaaaccgc actggctatc gatgccatca tcaaccagcg cgattccggt
    11941 atcaaatgta tctatgtcgc tatcggccag aaagcgtcca ccatttctaa cgtggtacgt
    12001 aaactggaag agcacggcgc actggctaac accatcgttg tggtagcaac cgcgtctgaa
    12061 tccgctgcac tgcaatacct ggcaccgtat gccggttgcg caatgggcga atacttccgt
    12121 gaccgcggtg aagatgcgct gatcatttac gatgacctgt ctaaacaggc tgttgcttac
    12181 cgtcagatct ccctgctgct ccgtcgtccg ccaggacgtg aagcattccc gggcgacgtt
    12241 ttctacctcc actctcgtct gctggagcgt gctgcacgtg ttaacgccga atacgttgaa
    12301 gccttcacca aaggtgaagt gaaagggaaa accggttctc tgaccgcact gccgattatc
    12361 gaaactcagg cgggtgacgt ttctgcgttc gttccgacca acgtaatctc cattaccgat
    12421 ggtcagatct tcctggaaac caacctgttc aacgccggta ttcgtcctgc ggttaacccg
    12481 ggtatttccg tatcccgtgt tggtggtgca gcacagacca agatcatgaa aaaactgtcc
    12541 ggtggtatcc gtaccgctct ggcacagtat cgtgaactgg cagcgttctc tcagtttgca
    12601 tccgaccttg acgatgcaac acgtaagcag cttgaccacg gtcagaaagt gaccgaactg
    12661 ctgaaacaga aacagtatgc gccgatgtcc gttgcgcagc agtctctggt tctgttcgca
    12721 gcagaacgtg gttacctggc ggatgttgaa ctgtcgaaaa ttggcagctt cgaagccgct
    12781 ctgctggctt acgtcgaccg tgatcacgct ccgttgatgc aagagatcaa ccagaccggt
    12841 ggctacaacg acgaaatcga aggcaagctg aaaggcatcc tcgattcctt caaagcaacc
    12901 caatcctggt aacgtctgga ggcttgcctt agggcaggcc gcaaggcatt gaggagaagc
    12961 tcatggccgg cgcaaaagag atacgtagta agatcgcaag cgtccagaac acgcaaaaga
    13021 tcactaaagc gatggagatg gtcgccgctt ccaaaatgcg taaatcgcag gatcgcatgg
    13081 cggccagccg tccttatgca gaaaccatgc gcaaagtgat tggtcacctt gcacacggta
    13141 atctggaata taagcaccct tacctggaag accgcgacgt taaacgcgtg ggctacctgg
    13201 tggtgtcgac cgaccgtggt ttgtgcggtg gtttgaacat taacctgttc aaaaaactgc
    13261 tggcggaaat gaagacctgg accgacaaag gcgttcaatg cgacctcgca atgatcggct
    13321 cgaaaggcgt gtcgttcttc aactccgtgg gcggcaatgt tgttgcccag gtcaccggca
    13381 tgggggataa cccttccctg tccgaactga tcggtccggt aaaagtgatg ttgcaggcct
    13441 acgacgaagg ccgtctggac aagctttaca ttgtcagcaa caaatttatt aacaccatgt
    13501 ctcaggttcc gaccatcagc cagctgctgc cgttaccggc atcagatgat gatgatctga
    13561 aacataaatc ctgggattac ctgtacgaac ccgatccgaa ggcgttgctg gataccctgc
    13621 tgcgtcgtta tgtcgaatct caggtttatc agggcgtggt tgaaaacctg gccagcgagc
    13681 aggccgcccg tatggtggcg atgaaagccg cgaccgacaa tggcggcagc ctgattaaag
    13741 agctgcagtt ggtatacaac aaagctcgtc aggccagcat tactcaggaa ctcaccgaga
    13801 tcgtctcggg ggccgccgcg gtttaaacag gttatttcgt agaggattta agatggctac
    13861 tggaaagatt gtccaggtaa tcggcgccgt agttgacgtc gaattccctc aggatgccgt
    13921 accgcgcgtg tacgatgctc ttgaggtgca aaatggtaat gagcgtctgg tgctggaagt
    13981 tcagcagcag ctcggcggcg gtatcgtgcg taccatcgca atgggttcct ccgacggtct
    14041 gcgtcgcggt ctggatgtaa aagacctcga acacccgatc gaagtcccgg taggtaaagc
    14101 gactctgggc cgtatcatga acgtactggg tgaaccggtc gacatgaaag gcgagatcgg
    14161 tgaagaagag cgttgggcga ttcaccgcgc agcaccttcc tacgaagagc tgtcaaactc
    14221 tcaggaactg ctggaaaccg gtatcaaagt tatcgacctg atgtgtccgt tcgctaaggg
    14281 cggtaaagtt ggtctgttcg gtggtgcggg tgtaggtaaa accgtaaaca tgatggagct
    14341 cattcgtaac atcgcgatcg agcactccgg ttactctgtg tttgcgggcg taggtgaacg
    14401 tactcgtgag ggtaacgact tctaccacga aatgaccgac tccaacgtta tcgacaaagt
    14461 atccctggtg tatggccaga tgaacgagcc gccgggaaac cgtctgcgcg ttgctctgac
    14521 cggtctgacc atggctgaga aattccgtga cgaaggtcgt gacgttctgc tgttcgttga
    14581 caacatctat cgttacaccc tggccggtac ggaagtatcc gcactgctgg gccgtatgcc
    14641 ttcagcggta ggttatcagc cgaccctggc ggaagagatg ggcgttctgc aggaacgtat
    14701 cacctccacc aaaactggtt ctatcacctc cgtacaggcg gtatacgtac ctgcggatga
    14761 cttgactgac ccgtctccgg caaccacctt tgcgcacctt gacgcaaccg tggtactgag
    14821 ccgtcagatc gcgtctctgg gtatctaccc ggccgttgac ccgctggact ccaccagccg
    14881 tcagctggac ccgctggtgg ttggtcagga acactacgac accgcgcgtg gcgttcagtc
    14941 catcctgcaa cgttatcagg aactgaaaga catcatcgcc atcctgggta tggatgaact
    15001 gtctgaagaa gacaaactgg tggtagcgcg tgctcgtaag atccagcgct tcctgtccca
    15061 gccgttcttc gtggcagaag tattcaccgg ttctccgggt aaatacgtct ccctgaaaga
    15121 caccatccgt ggctttaaag gcatcatgga aggcgaatac gatcacctgc cggagcaggc
    15181 gttctacatg gtcggttcca tcgaagaagc tgtggaaaaa gccaaaaaac tttaacgcct
    15241 taatcggagg gtgatatggc aatgacttac cacctggacg tcgtcagcgc agagcaacaa
    15301 atgttctctg gtctggtcga gaaaatccag gtaacgggta gcgaaggtga actggggatc
    15361 taccctggcc acgcaccgct gctcaccgcc attaagcctg gtatgattcg catcgtgaaa
    15421 cagcacggtc acgaagagtt tatctatctg tctggcggca ttcttgaagt gcagcctggc
    15481 aacgtgaccg ttctggccga caccgcaatt cgcggccagg atctcgacga agcgcgagcc
    15541 atggaagcga aacgtaaggc tgaagagcac attagcagct ctcacggcga cgtagattac
    15601 gctcaggcgt ctgcggaact ggccaaagcg atcgcgcagc tgcgcgttat cgagttgacc
    15661 aaaaaagcga tgtaacaccg gcttgaaaag cacaaaagcc agtctggaaa caggctggct
    15721 ttttttacgc gttgacccgt cctgaagagc gttcacatag atcctgctga tataaaacac
    15781 ccctgttttc ccgtttattc attgatcgaa ataagagcaa aaacatccac ctgacgctta
    15841 aatgaaggta ctgccttaat tttctgcaga taaaaggcgt gacgatggtc gaaaatggca
    15901 ctttcgtcag cggggataat ccgttattga acaatttatc ctctgtccat ttcacgatga
    15961 aaaaaatgta gttttttcaa ggtgaagcgg tttaaattcg ttctcaaatt acagtcagga
    16021 cgcgtatgtt gaataatgct atgagcgtag tgatccttgc cgcaggcaaa ggcacgcgca
    16081 tgtattccga tcttccgaaa gtgctgcata cccttgccgg gaaagcgatg gttcagcatg
    16141 tcattgatgc tgcgaatgaa ttaggcgcag cgcacgttca cctggtgtac ggtcacggcg
    16201 gcgatctgct taaacaggcg ctgaaagacg acaaccttaa ctgggtgctt caggcagagc
    16261 agctgggtac gggtcatgca atgcagcagg ccgcaccttt ctttgccgat gatgaagaca
    16321 ttttaatgct ctacggcgac gtgccgctga tctctgtcga aacactccag cgcctgcgtg
    16381 atgctaaacc ccagggtggc attggtctgc tgacggtgaa actggatgat ccgaccggtt
    16441 atggacgtat cacccgtgaa aatggcaaag ttaccggcat tgttgagcac aaagatgcca
    16501 ccgacgagca gcgtcagatt caggagatca acaccggcat tctgattgcc aacggcgcag
    16561 atatgaaacg ctggctggcg aagctgacca acaataatgc tcagggcgaa tactacatca
    16621 ccgacattat tgcgctggcg tatcaggaag ggcgtgaaat cgtcgccgtt catccgcaac
    16681 gtttaagcga agtagaaggt gtgaataacc gcctgcaact ctcccgtctg gagcgcgttt
    16741 accagtccga acaggctgaa aaactgctgt tagcaggcgt tatgctgcgc gatccggcgc
    16801 gttttgatct gcgcggtacg cttactcacg ggcgcgatgt tgaaattgat actaacgtta
    16861 tcatcgaggg caacgtgact ctcggtcatc gcgtgaaaat cggcaccggt tgcgtgatta
    16921 aaaacagcgt gattggcgat gattgcgaaa tcagtccgta taccgttgtg gaagacgcga
    16981 atctggcagc ggcctgtact attggcccgt ttgcccgtct gcgtcctggt gctgagttgc
    17041 tggaaggtgc acacgttggt aacttcgttg agatgaaaaa agcgcgtctg ggtaaaggca
    17101 cgaaagccgg gcatctgact tacctgggcg atgcggaaat tggcgataac gttaacatcg
    17161 gcgcgggaac cattacctgc aactacgatg gtgcgaataa atttaagacc attatcggcg
    17221 acgatgtgtt tgtcggttcc gacactcagc tggtggcccc ggtaacagta ggcaaaggcg
    17281 cgaccattgc tgcgggtaca actgtgacgc gtaatgtcgg cgaaaatgca ttagctatca
    17341 gccgtgtgcc gcagactcag aaagaaggct ggcgtcgtcc ggtaaagaaa aagtaattct
    17401 ggccgggtaa tccggtcatc tgggatgagg agataacata atctccctcc cacaagcagt
    17461 aactataaaa ataaccccac tctctacaag gctcggggcg cccgaaaaaa cgggcataca
    17521 ggttgaccga caacgatata aatcggaatc aaaaactatg tgtggaattg ttggcgcgat
    17581 cgcgcaacgt gatgtagcag aaatccttct tgaaggttta cgtcgtctgg aataccgcgg
    17641 atatgactct gccggtctgg ccgttgttga tgcggaaggt catatgactc gcctgcgtcg
    17701 cctcggtaaa gtccagatgc tggctcaggc agcggaagaa catcctctgc atggcggcac
    17761 cggtattgct cacactcgct gggcgaccca cggtgaacct tcagaagcta acgcgcatcc
    17821 gcatgtttct gaacacattg tggtggtgca taatggcatc atcgaaaacc atgaaccgct
    17881 gcgtgaagag ttaaaagcgc gtggctatac cttcgtttct gaaaccgaca ccgaagtgat
    17941 tgcccatctg gtgaactggg agctgaaaca aggcgggact ctgcgtgagg ctgttctgcg
    18001 tgctatcccg cagctgcgcg gtgcgtacgg tacagtgatc atggactccc gtcacccgga
    18061 taccctgcta gcggcacgtt ctggtagtcc gctggtgatt ggcctgggta tgggcgaaaa
    18121 ctttatcgct tctgaccagc tggcgctgtt gccggtgacc cgtcgcttta tcttccttga
    18181 agagggcgat attgcggaaa tcactcgccg ttcggtaaac atcttcgata aaactggcgc
    18241 ggaagtaaaa cgtcaggata tcgaatccaa tctgcaatat gacgcgggcg ataaaggtat
    18301 ttaccgtcac tacatgcaga aagagatcta cgaacagccg aacgcgataa aaaacaccct
    18361 taccgggcgc atcagccacg gtcaggttga tttaagcgag ctgggaccga acgccgacga
    18421 actgctgtcg aaggttgagc atattcagat cctcgcctgt ggtacttctt ataactccgg
    18481 tatggtttcc cgctactggt ttgaatcgct ggcaggtatt ccgtgcgacg tcgaaatcgc
    18541 ctctgaattc cgctatcgta aatctgccgt gcgtcgtaac agcctgatga tcaccttgtc
    18601 acagtctggc gaaaccgcgg ataccctggc gggcctgcgt ctgtcgaaag agctgggtta
    18661 ccttggttcg ctggcaatct gtaacgttcc gggttcttct ctggtgcgcg aatccgatct
    18721 ggcgctgatg accaacgcgg gtacggaaat cggcgtggcg tccactaaag cattcaccac
    18781 tcagttaact gtgctgttga tgctggtggc gaagctgtct cgcctgaaag gtctggatgc
    18841 ctccattgaa catgacattg tgcatggtct gcaggcgctg ccgagccgta ttgagcagat
    18901 gctgtctcag gataaacgca ttgaagctct ggcagaagat ttctctgaca aacatcacgc
    18961 gctgttcctg ggccgtggcg atcagtaccc aatcgcgctg gaaggcgcat tgaagctgaa
    19021 agagatctct tacattcacg ctgaagccta cgctgcaggc gagctgaaac acggcccgct
    19081 ggcgctgatt gacgcggata tgccggttat cgtcgttgca ccgaacaacg aactgttgga
    19141 aaaactgaaa tccaacattg aagaagttcg cgcgcgtggc ggtcaattgt atgtcttcgc
    19201 cgatcaggat gcgggttttg taagtagcga taacatgcac atcatcgaga tgccgcatgt
    19261 ggaagaggtg attgcaccga tcttctacac cgttccgctg cagctgctgg cttaccatgt
    19321 cgcgctgatc aaaggcaccg acgttgacca gccgcgtaac ctggcaaaat cggttacggt
    19381 tgagtaatca ctatcataga taaaaaaggg ctgctgtggg cggacaataa agtcttaaac
    19441 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
    19501 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
    19561 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
    19621 catggtaaag actatattcc atggctaaca gtacaagaag ttccttcttc aggtcgttcc
    19681 caccgtattt attctcataa gacgggacga gtccatcatt tgctatctga cttagagctt
    19741 gctgtttttc tcagtcttga gtgggagagc agcgtgctag atatacgcga gcagttcccc
    19801 ttattaccta gtgataccag gcagattgca atagatagtg gtattaagca tcctgttatt
    19861 cgtggtgtag atcaggttat gtctactgat tttttagtgg actgcaaaga tggtcctttt
    19921 gagcagtttg ctattcaagt caaacctgca gcagccttac aagacgagcg taccttagaa
    19981 aaactagaac tagagcgtcg ctattggcag caaaagcaaa ttccttggtt catttttact
    20041 gataaagaaa taaatcccgt agtaaaagaa aatattgaat ggctttattc agtgaaaaca
    20101 gaagaagttt ctgcggagct tttagcacaa ctatccccat tggcccatat cctgcaagaa
    20161 aaaggagatg aaaacattat caatgtctgt aagcaggttg atattgctta tgatttggag
    20221 ttaggcaaaa cattgagtga gatacgagcc ttaaccgcaa atggttttat taagttcaat
    20281 atttataagt ctttcagggc aaataagtgt gcagatctct gtattagcca agtagtgaat
    20341 atggaggagt tgcgctatgt ggcaaattaa tgaggttgtg ctatttgata atgatccgta
    20401 tcgcattttg gctatagagg atggccaagt tgtctggatg caaataagcg ctgataaagg
    20461 agttccacaa gctagggctg agttgttgct aatgcagtat ttagatgaag gccgcttagt
    20521 tagaactgat gacccttatg tacatcttga tttagaagag ccgtctgtag attctgtcag
    20581 cttccagaag cgcgaggagg attatcgaaa aattcttcct attattaata gtaaggatcg
    20641 tttcgaccct aaagtcagaa gcgaactcgt tgagcatgtg gtccaagaac ataaggttac
    20701 taaggctaca gtttataagt tgttacgccg ttactggcag cgtggtcaaa cgcctaatgc
    20761 attaattcct gactacaaaa acagcggtgc accaggggaa agacgttcag cgacaggaac
    20821 agcaaagatt ggccgagcca gagaatatgg taagggtgaa ggaaccaagg taacgcccga
    20881 gattgaacgc ctttttaggt tgaccataga aaagcacctg ttaaatcaaa aaggtacaaa
    20941 gaccaccgtt gcctatagac gatttgtgga cttgtttgct cagtattttc ctcgcattcc
    21001 ccaagaggat tacccaacac tacgtcagtt tcgttatttt tatgatcgag aataccctaa
    21061 agctcagcgc ttaaagtcta gagttaaagc aggggtatat aaaaaagacg tacgaccctt
    21121 aagtagtaca gccacttctc aggcgttagg ccctgggagt cgttatgaga ttgatgccac
    21181 gattgctgat atttatttag tggatcatca tgatcgccaa aaaatcatag gaagaccaac
    21241 gctttacatt gtgattgatg tgtttagtcg gatgatcacg ggcttttata tcggctttga
    21301 aaatccgtct tatgtggtgg cgatgcaggc ttttgtaaat gcttgctctg acaaaacggc
    21361 catttgtgcc cagcatgata ttgagattag tagctcagac tggccgtgtg taggtttgcc
    21421 agatgtgttg ctagcggacc gtggcgaatt aatgagtcat caggtcgaag ccttagtttc
    21481 tagttttaat gtgcgagtgg aaagtgctcc acctagacgt ggcgatgcta aaggcatagt
    21541 ggaaagcact tttagaacac tacaagccga gtttaagtcc tttgcacctg gcattgtaga
    21601 gggcagtcgg atcaaaagcc atggtgaaac agactatagg ttagatgcat ctctgtcggt
    21661 atttgagttc acacaaatta ttttgcgtac gatcttattc agaaataacc atctggtgat
    21721 ggataaatac gatcgagatg ctgattttcc tacagattta ccgtctattc ctgtccagct
    21781 atggcaatgg ggtatgcagc atcgtacagg tagtttaagg gctgtggagc aagagcagtt
    21841 gcgagtagcg ttactgcctc gccgaaaggt ctctatttct tcatttggcg ttaatttgtg
    21901 gggtttgtat tactcggggt cagagattct gcgtgagggt tggttgcagc ggagcactga
    21961 tatagctaga cctcaacatt tagaagcggc ttatgaccca gtgctggttg atacgattta
    22021 tttgtttccg caagttggca gccgtgtatt ttggcgctgt aatctgacgg aacgtagtcg
    22081 gcagtttaaa ggtctctcat tttgggaggt ttgggatata caagcacaag aaaaacacaa
    22141 taaagccaat gcgaagcagg atgagttaac taaacgcagg gagcttgagg cgtttattca
    22201 gcaaaccatt cagaaagcga ataagttaac gcccagtact actgagccca aatcaacacg
    22261 cattaagcag attaaaacta ataaaaaaga agccgtgacc tcggagcgta aaaaacgtgc
    22321 ggagcatttg aagccaagct cttcaggtga tgaggctaaa gttattcctt tcaacgcagt
    22381 ggaagcggat gatcaagaag attacagcct acccacatac gtgcctgaat tatttcagga
    22441 tccaccagaa aaggatgagt catgagtgct acccggattc aagcagttta tcgtgatacg
    22501 ggggtagagg cttatcgtga taatcctttt atcgaggcct taccaccatt acaagagtca
    22561 gtgaatagtg ctgcatcact gaaatcctct ttacagctta cttcctctga cttgcaaaag
    22621 tcccgtgtta tcagagctca taccatttgt cgtattccag atgactattt tcagccatta
    22681 ggtacgcatt tgctactaag tgagcgtatt tcggtcatga ttcgaggtgg ctacgtaggc
    22741 agaaatccta aaacaggaga tttacaaaag catttacaaa atggttatga gcgtgttcaa
    22801 acgggagagt tggagacatt tcgctttgag gaggcacgat ctacggcaca aagcttattg
    22861 ttaattggtt gttctggtag tgggaagacg acctctcttc atcgtattct agccacgtat
    22921 cctcaggtga tttaccatcg tgaactcaat gtagagcagg tggtgtattt gaaaatagac
    22981 tgctcgcata atggttcgct aaaagaaatc tgcttgaatt ttttcagagc gttggatcga
    23041 gccttgggct cgaactatga gcgtcgttat ggcttaaaac gtcatggtat agaaaccatg
    23101 ttggctttga tgtcgcaaat agccaatgca catgctttag ggttgttggt tattgatgaa
    23161 attcagcatt taagccgctc tcgttcgggt ggatctcaag agatgctgaa cttttttgtg
    23221 acgatggtga atattattgg cgtaccagtg atgttgattg gtacccctaa agcacgagag
    23281 atttttgagg ctgatttgcg gtctgcacgt agaggggcag ggtttggagc tatattctgg
    23341 gatcctatac aacaaacgca acgtggaaag cccaatcaag agtggatcgc ttttacggat
    23401 aatctttggc aattacagct tttacaacgc aaagatgcgc tgttatcgga tgaggtccgt
    23461 gatgtgtggt atgagctaag ccaaggagtg atggacattg tagtaaaact ttttgtactc
    23521 gctcagctcc gtgcgctagc tttaggcaat gagcgtatta ccgctggttt attgcggcaa
    23581 gtgtatcaag atgagttaaa gcctgtgcac cccatgctag aggcattacg ctcgggtatc
    23641 ccagaacgca ttgctcgtta ttctgatcta gtcgttcccg agattgataa acggttaatc
    23701 caacttcagc tagatatcgc agcgatacaa gaacaaacac cagaagaaaa agcccttcaa
    23761 gagttagata ccgaagatca gcgtcattta tatctgatgc tgaaagagga ttacgattca
    23821 agcctgttaa ttcccactat taaaaaagcg tttagccaga atccaacgat gacaagacaa
    23881 aagttactgc ctcttgtttt gcagtggttg atggaaggcg aaacggtagt gtcagaacta
    23941 gaaaagccct ccaagagtaa aaaggtttcg gctataaagg tagtcaagcc cagcgactgg
    24001 gatagcttgc ctgatacgga tttacgttat atctattcac aacgccaacc tgaaaaaacc
    24061 atgcatgaac ggttaaaagg gaaaggggta atagtggata tggcgagctt atttaaacaa
    24121 gcaggttagc catgagaaac tttcctgttc cgtactcgaa tgagctgatt tatagcacta
    24181 ttgcacgggc aggcgtttat caagggattg ttagtcctaa gcagctgttg gatgaggtgt
    24241 atggcaaccg caaggtggtc gctaccttag gtctgccctc gcatttaggt gtgatagcaa
    24301 gacatctaca tcaaacagga cgttacgctg ttcagcagct tatttatgag cataccttat
    24361 tccctttata tgctccgttt gtaggcaagg agcgccgaga cgaagctatt cggttaatgg
    24421 agtaccaagc gcaaggtgcg gtgcatttaa tgctaggagt cgctgcttct agagttaaga
    24481 gcgataaccg ctttagatac tgccctgatt gcgttgctct tcagctaaat aggtatgggg
    24541 aagccttttg gcaacgagat tggtatttgc ccgctttgcc atattgtcca aaacacggtg
    24601 ctttagtctt ctttgataga gctgtagatg atcaccgaca tcaattttgg gctttgggtc
    24661 atactgagct gctttcagac taccccaaag actccctatc tcaattaaca gcactagctg
    24721 cttatatagc ccctctgtta gatgctccac gagcgcaaga gctttcccca agccttgagc
    24781 agtggacgct gttttatcag cgcttagcgc aggatctagg gctaaccaaa agcaagcaca
    24841 ttcgtcatga cttggtggcg gagagagtga ggcagacttt tagtgatgag gcactagaga
    24901 aactggattt aaagttggca gagaacaagg acacgtgttg gctgaaaagt atattccgta
    24961 agcatagaaa agcctttagt tatttacagc atagtattgt gtggcaagcc ttattgccaa
    25021 aactaacggt tatagaagcg ctacagcagg caagtgctct tactgagcac tctataacga
    25081 caagacctgt tagccagtct gtgcaaccta actctgaaga tttatctgtt aagcataaag
    25141 actggcagca actagtgcat aaataccaag gaattaaggc ggcaagacag tctttagagg
    25201 gtggggtgct atacgcttgg ctttaccgac atgacaggga ttggctagtt cactggaatc
    25261 aacagcatca acaagagcgt ctggcacccg cccctagagt tgattggaac caaagagatc
    25321 gaattgctgt acgacaacta ttaagaatca taaagcgtct agatagtagc cttgatcacc
    25381 caagagcgac atcgagctgg ctgttaaagc aaactcctaa cggaacctct cttgcaaaaa
    25441 atctacagaa actgcctttg gtagcgcttt gcttaaagcg ttactcagag agtgtggaag
    25501 attatcaaat tagacggatt agccaagctt ttattaagct taaacaggaa gatgttgagc
    25561 ttaggcgctg gcgattatta agaagtgcaa cgttatctaa agagcggata actgaggaag
    25621 cacaaagatt cttggaaatg gtttatgggg aagagtgagt ggttaggcta gctacattta
    25681 atgacaatgt gcaggttgta catattggtc atttattccg taactcgggt cataaggagt
    25741 ggcgtatttt tgtttggttt aatccaatgc aagaacggaa atggactcga tttactcatt
    25801 tgcctttatt aagtcgagct aaggtggtta acagtacaac aaagcaaata aataaggcgg
    25861 atcgtgtgat tgagtttgaa gcatcggatc ttcaacgagc caaaataatc gattttccta
    25921 atctctcgtc ctttgcttcc gtacgcaaca aggatggagc gcagagttca tttatttacg
    25981 aagctgaaac accatatagc aagactcgtt atcacatccc acagttagag ctagctcggt
    26041 cattattttt aattaactcc tatttctgtc gaagctgttt gagcagtacc gctttacagc
    26101 aagagttcga cgttcagtat gaggttgagc gagatcattt agagataagg atcttaccca
    26161 gttcatcgtt tcctaaaggg gcgttagagc tgtcggccgt agtgcagctt ttggtttggt
    26221 tgttttcgga tcaagatgtt atggattcgt atgaaagtat ttttaggcac tatcaacaaa
    26281 atagagaaat taagaacggc gttgaaagct ggtgctttag ctttgaccct ccgcccatgc
    26341 agggttggaa attacatgta aaaggacgtt cttctaacga ggataaggat tatttagttg
    26401 aggaaatagt aggtttagaa atcaacgcta tgcttcctag cacaacagct attagccatg
    26461 cctcttttca ggaaaaggag gcaggtgatg gtagtacgca gcacatagcg gtttcaacag
    26521 agtcagttgt tgatgatgag catctacagt tggacgatga ggaaacagcc aatatagaca
    26581 cagacacacg agtcatagag gctgagccga catggataag ttttagtaga cctagtcgaa
    26641 ttgaaaaatc tcgcagggca agaaaaagta gccaaactat tttagaaaaa gaagaagcaa
    26701 caacaagtga aaatagtaat ttggttagta ctgatgagcc acacttaggt ggtgtcctag
    26761 cagcggcaga tgtgggtggg aagcaggatg caaccaatta caactctatt tttgctaatc
    26821 gatttgctgc ttttgatgag ctactttcaa ttctaaaaac taaatttgca tgtcgggtgc
    26881 tttttgaaga aaccttggtt ttgccaaaag ttgggcgtag ccgattacat ctgtgtaaag
    26941 atggctcacc aagagtgatt aaagccgttg gggtgcaacg taatggcagt gaatttgtat
    27001 tgctagaggt ggatgcatcg gatggggtga aaatgctttc taccaaagtg ttgagtggcg
    27061 ttgatagcga aacatggcgg aatgattttg aaaagatacg gcgtggagtg gtgaagagct
    27121 cattgaattg gccaaatagt ttgtttgatc aattatatgg acaagacggg catagagggg
    27181 tgaatcatcc aaaggggttg ggggagctgc aagtatcgag agaggatatg gaagggtggg
    27241 ctgagagagt ggttagagag caatttacgc attaaaggaa tgactgaaag agcctgtaaa
    27301 cccttttgtg taagtgcttt tgccggtcag ttaaaggtgg ccatttaaac ggtcaccaaa
    27361 ttcgatcata aaacggttca tggccggctt ccagttgcgg atcggcatcg tccatttctt
    27421 ggtcgccgcc tggatagcca ggtacaccac cttcatcgct gattcgtccg tagggaacac
    27481 cttgagttca tattaatgga attttctaca aatagcctcc gtggttttga ggggggatta
    27541 cagacgatcc atagtagtaa tccaatgagt tcttgagcgc ggcgacatgt ttggacgcct
    27601 tggcaaaaat tagagcctgc ttgaagtgca ggcgagcacg tgcttggacg attgatccat
    27661 tcgcggtcaa aaactcaatc ttggatgaca gcgtgtcagg aaatccaata tcgaagtccc
    27721 gccccgtgtc aaagtagggc atttcatgat caaaggacga agcttcccag gccacttccc
    27781 taatattcca gccgctagaa acaagatttc gtacgacttc atcaactttt ttgctttcca
    27841 acttgtcaca aagtgcatag gcaagagctt tggcggcaga cacgtgcgtt tcctcttaaa
    27901 cataggtcga acggataccc gaggcaaagc ggagcattag tcccaaagca tctccggtag
    27961 atccggaggc tccatactga aggcatggca tcttgggcat tgatatttga aacttctctc
    28021 tagatagcag ggatttcttg gccttgcctc atgctgatag cttgtaaata tcacacgatc
    28081 cttagcccca caaagtgagc atactccgta gctttcattt atagaaatcg tcgttagatt
    28141 gtcgcagcat gtgcacgttc cgactatttg ctcaggaagc atc
//
LOCUS       DAZOZX010000043        27760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZOZX010000043 DAZOZX010000000
VERSION     DAZOZX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.043"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            255..2276
                     /gene="uvrB"
                     /locus_tag="ACHUPT_001797"
     CDS             255..2276
                     /gene="uvrB"
                     /locus_tag="ACHUPT_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0819394.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(2468..3376)
                     /gene="yvcK"
                     /locus_tag="ACHUPT_001798"
     CDS             complement(2468..3376)
                     /gene="yvcK"
                     /locus_tag="ACHUPT_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0819395.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      3641..3785
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3773..4762
                     /gene="moaA"
                     /locus_tag="ACHUPT_001799"
     CDS             3773..4762
                     /gene="moaA"
                     /locus_tag="ACHUPT_001799"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0819396.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            4784..5296
                     /gene="moaB"
                     /locus_tag="ACHUPT_001800"
     CDS             4784..5296
                     /gene="moaB"
                     /locus_tag="ACHUPT_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0819397.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            5299..5784
                     /gene="moaC"
                     /locus_tag="ACHUPT_001801"
     CDS             5299..5784
                     /gene="moaC"
                     /locus_tag="ACHUPT_001801"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0819398.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            5777..6022
                     /gene="moaD"
                     /locus_tag="ACHUPT_001802"
     CDS             5777..6022
                     /gene="moaD"
                     /locus_tag="ACHUPT_001802"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0819399.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            6024..6476
                     /gene="moaE"
                     /locus_tag="ACHUPT_001803"
     CDS             6024..6476
                     /gene="moaE"
                     /locus_tag="ACHUPT_001803"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0819400.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            6613..7317
                     /locus_tag="ACHUPT_001804"
     CDS             6613..7317
                     /locus_tag="ACHUPT_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0819401.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            7522..8235
                     /locus_tag="ACHUPT_001805"
     CDS             7522..8235
                     /locus_tag="ACHUPT_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0819402.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(8271..9227)
                     /locus_tag="ACHUPT_001806"
     CDS             complement(8271..9227)
                     /locus_tag="ACHUPT_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR0819403.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(9227..10468)
                     /gene="clsB"
                     /locus_tag="ACHUPT_001807"
     CDS             complement(9227..10468)
                     /gene="clsB"
                     /locus_tag="ACHUPT_001807"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0819404.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(10465..11226)
                     /locus_tag="ACHUPT_001808"
     CDS             complement(10465..11226)
                     /locus_tag="ACHUPT_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0819405.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            11359..11769
                     /locus_tag="ACHUPT_001809"
     CDS             11359..11769
                     /locus_tag="ACHUPT_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0819406.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(11731..12837)
                     /locus_tag="ACHUPT_001810"
     CDS             complement(11731..12837)
                     /locus_tag="ACHUPT_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0819407.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12848..13981)
                     /locus_tag="ACHUPT_001811"
     CDS             complement(12848..13981)
                     /locus_tag="ACHUPT_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0819408.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(13974..15710)
                     /locus_tag="ACHUPT_001812"
     CDS             complement(13974..15710)
                     /locus_tag="ACHUPT_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0819409.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(15703..16698)
                     /gene="hlyD"
                     /locus_tag="ACHUPT_001813"
     CDS             complement(15703..16698)
                     /gene="hlyD"
                     /locus_tag="ACHUPT_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0819410.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(16701..17372)
                     /gene="cecR"
                     /locus_tag="ACHUPT_001814"
     CDS             complement(16701..17372)
                     /gene="cecR"
                     /locus_tag="ACHUPT_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR0819411.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            17601..18821
                     /gene="rhlE"
                     /locus_tag="ACHUPT_001815"
     CDS             17601..18821
                     /gene="rhlE"
                     /locus_tag="ACHUPT_001815"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0819412.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(18815..18913)
                     /locus_tag="ACHUPT_001816"
                     /pseudo
     CDS             complement(18815..18913)
                     /locus_tag="ACHUPT_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18995..19477)
                     /gene="ybiA"
                     /locus_tag="ACHUPT_001817"
     CDS             complement(18995..19477)
                     /gene="ybiA"
                     /locus_tag="ACHUPT_001817"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0819413.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            19597..21747
                     /gene="dinG"
                     /locus_tag="ACHUPT_001818"
     CDS             19597..21747
                     /gene="dinG"
                     /locus_tag="ACHUPT_001818"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0819414.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            21775..22737
                     /gene="ybiB"
                     /locus_tag="ACHUPT_001819"
     CDS             21775..22737
                     /gene="ybiB"
                     /locus_tag="ACHUPT_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0819415.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            22878..23963
                     /gene="hcxB"
                     /locus_tag="ACHUPT_001820"
     CDS             22878..23963
                     /gene="hcxB"
                     /locus_tag="ACHUPT_001820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0819416.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            24030..24089
                     /gene="ybiE"
                     /locus_tag="ACHUPT_001821"
     CDS             24030..24089
                     /gene="ybiE"
                     /locus_tag="ACHUPT_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0819417.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(24192..24452)
                     /gene="ybiJ"
                     /locus_tag="ACHUPT_001822"
     CDS             complement(24192..24452)
                     /gene="ybiJ"
                     /locus_tag="ACHUPT_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0819418.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(24717..24983)
                     /gene="ybiI"
                     /locus_tag="ACHUPT_001823"
     CDS             complement(24717..24983)
                     /gene="ybiI"
                     /locus_tag="ACHUPT_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0819419.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(25057..25734)
                     /gene="ybiX"
                     /locus_tag="ACHUPT_001824"
     CDS             complement(25057..25734)
                     /gene="ybiX"
                     /locus_tag="ACHUPT_001824"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0819420.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(25776..27760)
                     /locus_tag="ACHUPT_001825"
                     /pseudo
     CDS             complement(25776..27760)
                     /locus_tag="ACHUPT_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         6528 a         7174 c         7448 g         6610 t
ORIGIN      
        1 tctcttgcca taaaactgtc atcactcatc ttgacaaatg ttaaaaaagc cgttgctttg
       61 gggataaccc ggtaaggctg gagttttatc tcgccacaga gtaaattttg ctcatgattg
      121 acagcggagt ttacgctgta tcagaaatat tatggtgatg aactgttttt ttatccagta
      181 taatttgttg gcataattaa gtacgacgag taaaattaca tacctgcccg cccaactcct
      241 tcaggtagcg actcatgagt aaaccgttca aactgaattc cgcttttaaa ccttctggcg
      301 atcagccaga ggcgattcga cgtctcgaag aggggctgga agatggcctg gcgcaccaga
      361 cgttacttgg cgtgactggc tccgggaaaa ccttcaccat tgccaatgtc attgctgacc
      421 ttcagcgccc aaccatggta cttgcgccca acaaaacgct ggcggcccag ctgtatggcg
      481 aaatgaaaga gttcttcccg gaaaacgcgg tggagtattt cgtctcctac tacgactact
      541 atcagccgga agcctatgta ccgagttccg acaccttcat tgagaaagat gcctcggtta
      601 acgaacatat tgagcagatg cgtttgtccg ccaccaaagc gatgctggag cggcgtgatg
      661 tgattgtagt ggcgtctgtt tccgcgattt atggtctggg cgatcctgat ttatatctca
      721 agatgatgct ccatctcacg gtcggtatga ttatcgatca gcgcgcgatt ctgcgccgac
      781 tggcggagct gcaatacgct cgtaatgatc aagcattcca gcgtggtact ttccgcgttc
      841 gtggcgaggt gatagatatc ttcccggcag aatcggatga cattgcactt cgcgtggaac
      901 tgtttgacga ggaagtggaa cgattgtcgt tatttgaccc gctgaccggg cagattgttt
      961 ccactattcc acgttttacc atctacccga aaacgcacta cgtcacaccg cgcgagcgca
     1021 tcgtacaggc gatggaggag atcaaagaag aactggccgc cagacgcaaa gtgctgttgg
     1081 aaaacaacaa actgctggaa gagcagcggc tgacccagcg tacccagttt gatctggaga
     1141 tgatggacga gctgggctac tgttcgggga ttgaaaacta ctcgcgcttc ctctccggtc
     1201 gtggaccggg tgagccaccg ccgacgctgt ttgattacct gcctgccgat gggctgctgg
     1261 tcgtcgatga atctcacgtc accattccac aaattggcgg catgtatcgc ggtgaccggg
     1321 cgcgtaaaga gacactggtg gagtacggct tccgcctgcc atcagcgctg gataaccgtc
     1381 cgcttaagtt tgaagagttc gaagcattag cgccgcaaac catctatgtt tcggcgacgc
     1441 cgggtaatta cgagctggaa aaatccggcg gcgatgtggt ggatcaggtg gtgcgtccaa
     1501 ccggattgct tgacccgatt atcgaagtgc ggccggtggc gacacaggtt gatgatcttc
     1561 tttcggagat tcgtcagcga gcggcaatta acgaacgcgt gctggtcacc acactgacca
     1621 agcgaatggc ggaagatctt accgaatatc tcgaagaaca cggtgagcgc gtgcgttatc
     1681 ttcactcaga tatcgacacc gtcgagcgta tggagattat ccgcgacttg cgtctgggtg
     1741 agttcgacgt attggtaggg atcaacttac tgcgtgaagg tctggatatg ccggaagtgt
     1801 cgctggtggc gatcctcgac gctgacaaag aaggcttcct gcgttccgaa cgttcgttga
     1861 tccagaccat tggtcgtgcg gcacgtaacg ttaacggtaa agcgattctc tacggcgata
     1921 ggagcactcc atcaatggcg aaagcgattg gcgaaaccga acgtcgccgt gagaaacagc
     1981 agaagtacaa cgaggaacac ggcattacgc cgcaaggctt gaacaagaaa gtggtcgata
     2041 tcctggcgct ggggcagaac attgccaaaa ccaaagcgaa gggcagagga aaatcgcgcc
     2101 cgattgttga gccggataat gtgccgatgg atatgttgcc gaaagcgttg cagcagaaaa
     2161 tccatgaact ggaagggctg atgatgcaac acgcgcagaa tctggagttc gaagaagcgg
     2221 cacaaattcg tgaccagttg catcagttgc gtgagttgtt tattgccgca tcgtaacagg
     2281 atagcgaaga agactgatga caaacggaaa acagtctgat gcgctacgct tatcaggcct
     2341 acattttctc cgcaatatat tgaatttgcg cggtttgtag gccggataag gcgttcacgc
     2401 cgcatccggc ataaacaacg cgcactttgt cttcaatagg aagccggaat tttccttcgg
     2461 atttccgtta acctaaagcc tgtaacgcct tttccagcgc gttatgtaac aactggcggt
     2521 catgacgata cggaatatcg ctggcctcca gtacctcctg gatcacaatc cgctctttca
     2581 ccgccgagac atccactttt ggcccgacga tgaccgcatc aatgaccttt ttaccaacat
     2641 actgctccat aattgccagc ttgctttcca gcttcaaatt agccgcaggt aaactcaact
     2701 cacgccccag attgccgata taaaccatcg gcgctggcgt gcggcgtaat gcctgggcga
     2761 tttccttcag cagcagaatt ggcatcaggc tggtataaaa actgccaggc ccaataatga
     2821 tgagatccgc ttcattgata gcgtgaaccg cctcacgcgt tgcgggtaca ttaggcgtta
     2881 acaataactc ttgaatcggc gtagttaact ggtcgatatt gacctcgccg taaacttcat
     2941 gcccctgatc gtcaatcgcc atcagatcaa caggatgctc tgacattgga atcaaatgcg
     3001 tatccacttt cagcagatta cgaattaaat tgatggcttc cagaggccgc acgctaaggt
     3061 gatccagcgc ctttaacatc aagtttccga gattatgacc ggaaagttcg ccattgccgc
     3121 caaaacggta ttcaaacatc gcggaggcga cgttcggttc cgttatcagc tggttgaggc
     3181 agttgcgcat atcgccccaa gcaatgccgc cttctgaacg gcgaatacgc cccgtcgagc
     3241 caccattatc cgtggtggtg acgatacccg ttaaacgaga acccaaagac gaaagtgatg
     3301 agagaacgcg tcccagtcca tgccctccgc cgagagcaac gacacgatca agatcagcca
     3361 gcgtacgatt gcgcatatag tttcctggag tcagattatc cgcgctacag tagcgcaaat
     3421 tgcggggaaa cagcaattaa cctgccaata ttgaggatac aaactatttt cttctatctc
     3481 taaaggacga tgcacgctat gcctccctga tgatgtatat caaagtaaaa ccgccatttt
     3541 cccttattct gtagcgaaat agcacgatca tgacgctata tacatgatta catagcgaaa
     3601 gtgtggatgg taaaaatctc atttacacgc tagtatcggc ttaaccacta aacactctag
     3661 cctctgcacc tgggtcaact gatacggtgc tttggccgtg acaatgctcg taaagattgc
     3721 caccagggcg aaggaagaaa tgacttcgcc tcccgtatct ggaaaggtgt acatggcttc
     3781 acaactgact gatgtatttg cgcgtaagtt ttactacttg cgcctgtcga ttaccgatgt
     3841 gtgtaacttt cgttgcacct actgcctgcc ggatggctac aaaccgagcg gcgtcaccaa
     3901 taaaggcttt cttaccgtcg atgaaattcg ccgggttacg cgcgccttcg ccagtctggg
     3961 caccgaaaaa gtgcgcctga caggaggaga gccgtcttta cgccgtgact ttaccgatat
     4021 catcgccgct gtgcgggaaa acgacgctat ccgccagatt gcggtcacca ccaatggtta
     4081 ccgtctggaa cgcgatgtgg cgaactggcg cgatgcggga cttactggca ttaacgtcag
     4141 tgtcgacagt ttggacgccc gccagtttca cgctattacc gggcaggata aattcaacca
     4201 ggtcatggca gggattgatg ctgcatttga ggccggtttt gagaaggtca aagtcaatac
     4261 cgtgctgatg cgtgatgtta atcatcacca gctcgacacc tttctgaact ggatccagca
     4321 tcgccctatc cagctgcgtt tcatcgaact gatggaaacg ggcgagggca gcgagctctt
     4381 ccgtaagcat cacatctctg gtcaggttct gcgtgacgag ctactgcgtc gcggctggat
     4441 ccaccaatta cgtcaacgca gcgacggtcc cgcgcaagtc ttttgccatc cagattacgc
     4501 cggagagatt ggccttatca tgccgtatga aaaagacttc tgcgccactt gcaaccgcct
     4561 gcgcgtttcc tccattggta aactccatct ctgcctgttt ggtgaaggcg gcgttaacct
     4621 gcgcgatctg ctggaaggcg atacccagca acaggcgctg gaagcgcgta tttcagcggc
     4681 gctgcgggag aagaaacaga cccatttcct gcatcaaaac aacaccggta ttacgcaaaa
     4741 cttatcgtac attggcggct aaaacgtcaa aaggagagat cagatgagtc aggtaagcac
     4801 tgaatttatc ccgacccgta ttgctattct tacggtttct aatcgtcgcg gtgaagaaga
     4861 cgatacctcc ggtcactatc tgcgcgattc ggcgcaagaa gcgggccatc acgttgtcga
     4921 tgaagccatt gtgaaagaaa accgctacgc tattcgcgct caggtatctg cgtggatcgc
     4981 cagcgacgat gtacaagtgg tattgattac gggtggtact ggcctgacgg aaggtgatca
     5041 ggctcccgaa gcgctgctgc cgttgttcga ccgtgaggtt gaaggttttg gtgaagtgtt
     5101 ccgtatgttg tcgtttgaag agattggcac ttccacgttg caatctcgtg cggtagcggg
     5161 cgtcgctaac aaaacgctga ttttcgccat gccgggttcg accaaagcgt gccgtaccgc
     5221 atgggaaaat atcatcgcgc cgcagctgga tgcccgtacg cgtccgtgta atttccatcc
     5281 acatttgaag aaataagtat gtcgcaactg acccatatca acgccgctgg cgaagcgcac
     5341 atggtggatg tctccgccaa agcggaaacc gtgcgtgaag cgcgggcgga agcctttgtc
     5401 accatgcgca gcgagacgct ggcgatgatt attgatggtc gccaccacaa aggcgacgta
     5461 tttgccactg cgcgtattgc cggtattcag gcggcaaaac gcacttggga tctgatcccg
     5521 ctgtgtcacc cgctgatgct cagcaaagtt gaagtcaatt tacaggccga gccggagcac
     5581 aatcgggtgc gtatagaaac cttatgccgc ctgaccggga aaaccggtgt cgaaatggaa
     5641 gcgttaaccg cggcctccgt ggcggcgctg accatttatg acatgtgcaa agcggtgcaa
     5701 aaagatatgg tgattggtcc ggtacgtttg ctggcgaaga gcggcggcaa gtcgggtgac
     5761 tttaaggtgg aagcggatga ttaaagttct ttttttcgcc caggtgcgcg agttggtggg
     5821 aacagatgca accgaagtgg ctgcggatta cccaaccgtt gaagcgttac gccagcacct
     5881 ggctgcgcag agcgatcgct gggcgctggc gctggaagat ggcaaattgc tggctgccgt
     5941 caaccagacg ctggtgagtt ttgaccatcc gctgactgac ggcgacgaag tagctttctt
     6001 cccgccggta accggaggtt aagatggcag aaaccaaaat tgttgtcggc ccgcagccgt
     6061 tcagcgtagg agaagagtac ccgtggctgg cggagcgtga cgaagacggt gcggtagtca
     6121 ccttcaccgg aaaggtgcgc aaccataacc tgggcgacag cgtcaaagca ttaaccctcg
     6181 aacactatcc ggggatgact gaaaaagcac tggcagaaat tgttgatgaa gcgcgtaacc
     6241 gctggccgct ggggcgcgtc acggtgattc accgcatcgg ggaattatgg ccgggcgatg
     6301 aaatcgtttt tgtcggtgtc accagtgcgc atcgcagcag tgcgtttgaa gccgggcagt
     6361 ttattatgga ttatctcaaa acccgcgcac cgttctggaa gcgcgaagcc acgccggaag
     6421 gcgaccgctg ggttgaagcc cgggagagcg atctgcaggc ggcaaaacgc tggtagtttt
     6481 ttgttagccg gataaggcac cgctgcgtcc ggcaatttca ccgctcagta catgcgttta
     6541 cgctgcgtcg atgcacagcc tcatcatttt gcagtatcct taagatattc cttatatctt
     6601 caggagatcg tcatggacag attcccacgt tctgattcaa tcgtacaacc ctgggctggc
     6661 ttgcaaactt atatggcgca agtctatggc tggatgaccg ttggcttgtt gctgaccgca
     6721 tttgttgcct ggtatgcggc taattccgcg gccgtgatgg agctgttgtt cactaaccgt
     6781 gtctttttaa tcggtctgat catcgcgcaa ttagcattgg ttattgtgtt atcagcgatg
     6841 attcaaaagc tgagcgcagg tgtaacgacg atgctcttta tgctttattc ggcgctgacg
     6901 ggtcttacgc tttccagtat attcattgtc tataccgctg cttctatcgc cagtactttc
     6961 gtcgttactg ccgggatgtt cggcgcaatg agcctgtacg gttacaccac gaagcgcgat
     7021 ttaagtggct ttggcaatat gctgtttatg gcgttaatcg gcattgtgct ggcatcgctg
     7081 gtcaacttct ggctgaaaag cgaagcattg atgtgggcag ttacctacat cggcgtgatt
     7141 gtctttgtcg gattgacggc gtatgacact cagaaattga aaaatatggg tgagcagatt
     7201 gatacccgcg acacgtcgaa cctgcgcaaa tattccattc ttggtgcgtt aaccttgtat
     7261 ctggacttca tcaacctgtt cctgatgttg ttacggatct tcggcaaccg ccgttaattc
     7321 tttttcacca tcactatccc ggccatgtgt cgggattttt ttcttactaa aaacgacact
     7381 taacaagtac caggtataaa atatgcctta tgagcgagcg gaatcgtttt ccgtatccat
     7441 aaatacaaaa gtattttgta tgcgtggttt taaatttatt gatggtgaat attaatgttt
     7501 tttctaagga caactaaaag aatggaatca tactcgcaaa acagcaataa attagatttt
     7561 cagcacgagg ccaggatatt aaacggtata tggctcatta ccgctttagg tttggtggca
     7621 accgcaggac tagcctgggg agctaagtat ctcgaaatta cggcaaccaa atatgattca
     7681 ccaccaatgt atgtcgccat agggttatta ttactttgta tgtatggctt aagtaaggat
     7741 atcaacaaga taaatgccgc catcgcgggc gtaatatatc tgtttttact ctctttggtg
     7801 gcgattgtcg ttgcaagttt agttcctgta tatgccatta tcatcgtgtt cagcactgcg
     7861 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
     7921 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
     7981 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
     8041 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
     8101 gagttgtgga gtccggttcg ttgcgctttt acaggtgcat taacactcta ttactatttt
     8161 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
     8221 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
     8281 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
     8341 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggcgataatg
     8401 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
     8461 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
     8521 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
     8581 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
     8641 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
     8701 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
     8761 taatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
     8821 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     8881 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     8941 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     9001 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     9061 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     9121 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     9181 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag gggtttaccc
     9241 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     9301 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     9361 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     9421 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     9481 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     9541 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     9601 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     9661 gcaagcgcgc accgactctg acaatcggca tatccggttc gccctgaatg atcagtttga
     9721 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     9781 agtaggcgtt ggcgataatc acttcccgct gcgcctgagt gagcattttc agataatggc
     9841 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     9901 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     9961 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
    10021 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
    10081 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
    10141 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
    10201 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
    10261 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
    10321 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
    10381 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
    10441 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
    10501 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
    10561 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
    10621 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
    10681 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
    10741 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
    10801 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
    10861 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
    10921 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
    10981 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
    11041 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
    11101 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
    11161 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
    11221 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
    11281 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
    11341 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
    11401 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
    11461 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
    11521 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
    11581 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
    11641 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
    11701 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
    11761 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
    11821 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
    11881 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
    11941 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
    12001 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
    12061 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
    12121 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
    12181 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
    12241 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
    12301 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
    12361 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
    12421 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
    12481 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
    12541 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
    12601 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
    12661 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
    12721 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
    12781 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
    12841 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
    12901 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
    12961 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
    13021 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
    13081 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
    13141 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
    13201 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
    13261 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
    13321 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
    13381 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
    13441 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
    13501 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
    13561 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
    13621 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
    13681 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
    13741 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
    13801 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
    13861 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
    13921 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
    13981 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
    14041 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
    14101 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
    14161 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
    14221 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
    14281 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
    14341 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
    14401 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
    14461 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
    14521 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
    14581 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
    14641 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
    14701 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
    14761 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
    14821 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
    14881 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgcca
    14941 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
    15001 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
    15061 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
    15121 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
    15181 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
    15241 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
    15301 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
    15361 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
    15421 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
    15481 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
    15541 agtaatcccg ccaacatccg catcagtgtg gttttacctg caccgtccgg ccccaccaac
    15601 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
    15661 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
    15721 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
    15781 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
    15841 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
    15901 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
    15961 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
    16021 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
    16081 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
    16141 gctggctttc gcctgagcga tgtcctgttc acggttaccg gaacggtact gacgcaattt
    16201 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
    16261 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
    16321 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
    16381 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
    16441 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
    16501 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
    16561 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
    16621 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
    16681 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
    16741 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
    16801 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
    16861 gcatgggtat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
    16921 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
    16981 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
    17041 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
    17101 tcaggctgtg gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg
    17161 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
    17221 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
    17281 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
    17341 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
    17401 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
    17461 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
    17521 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
    17581 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
    17641 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
    17701 ctgtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
    17761 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
    17821 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    17881 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    17941 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    18001 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    18061 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    18121 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    18181 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    18241 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    18301 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    18361 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    18421 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    18481 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    18541 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    18601 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    18661 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    18721 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    18781 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    18841 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    18901 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    18961 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    19021 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    19081 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    19141 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    19201 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    19261 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    19321 cctgaaaata gtgttcggag gtgggccagg tattcccgtc aaccttgatg ggcctggcgg
    19381 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    19441 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    19501 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    19561 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    19621 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    19681 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    19741 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    19801 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    19861 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    19921 ttcactgccg ctttcgggcg tgggcgctac gtttgtccac gtaatctgac ggcgctcgcc
    19981 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    20041 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    20101 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    20161 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    20221 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    20281 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    20341 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    20401 gtgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    20461 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    20521 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    20581 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    20641 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    20701 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    20761 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    20821 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    20881 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    20941 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    21001 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    21061 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    21121 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    21181 gtggagagca aaaaatatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    21241 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    21301 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    21361 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    21421 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    21481 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    21541 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    21601 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    21661 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    21721 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    21781 tatcgcaaaa tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    21841 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    21901 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt
    21961 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg
    22021 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg
    22081 attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact
    22141 cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg
    22201 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc
    22261 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
    22321 accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagcgtt
    22381 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg
    22441 ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc
    22501 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc
    22561 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg
    22621 gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
    22681 acgggtgaag cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt
    22741 ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct
    22801 gcatctttat tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca aacacataac
    22861 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
    22921 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
    22981 atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa
    23041 gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta
    23101 aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac
    23161 acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc
    23221 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg
    23281 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
    23341 gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt
    23401 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
    23461 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
    23521 ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata
    23581 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa
    23641 cgacgcatca ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta
    23701 tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
    23761 ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt
    23821 gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct
    23881 ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt
    23941 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
    24001 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
    24061 cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg
    24121 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc
    24181 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
    24241 gaagtgatgc tgtatccact ggcaccggct gccgccgctt tctcagccag tttcgcttcc
    24301 agggcatcga ggggggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt
    24361 gcctgggatg ccgttaccgg ttccgccgcg aacacgccaa atgacagagt tgaaagagcc
    24421 atagcagcaa caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta
    24481 taagtgacgt tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg
    24541 aagagaattg accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat
    24601 ttatttgttg tacttatgga cttgttttat gccgcgtggc agtcacagca gcgttaaagg
    24661 cacggaatgc cacgcaaaat acaaattata ctcgctatgc atctcgccag ttgtagttaa
    24721 cgtaactgtc tgtctttcga acctctgcga ttatatcctg tatacgctgg tttttgtaaa
    24781 tctttctcct gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggct
    24841 tgcgggatgg gggcaccgca ctcttcacat tcatacaggc tttcgccacg cggaatttca
    24901 ccccgagcgc gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca
    24961 tcgttagccc aagcggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg
    25021 tagctaatat agggacgaac ctgggcagtt caagcgtcag atctccgacc attcccgcag
    25081 cagattatga taaagattga gcagcgacag gatctcttca ttttcaccat agcggctttt
    25141 cagcgactga atatttttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat
    25201 catcgactgg atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca
    25261 atgcaggctg ctggagggat acaacaccag atcacctgcc gggagtttta cccgatgttg
    25321 tccaaaggtg tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa
    25381 cagcgtggca gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc
    25441 atccacatgg aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag
    25501 ggtacgcggc aaggcagcgg caaagaacaa tgcatgttgg ttaaccgcgt tcagcacctc
    25561 attttgcagt gcggcgtata acgcgctgcg ggtgtctact tgctgattgt tcttaacttg
    25621 tgcaccttgt gctccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc
    25681 gcgaaaatgg gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt
    25741 tctccaaaaa gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc
    25801 aagaaggttc ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg
    25861 taatcggtat caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga
    25921 taccccagtt tggcatcggc gacccagtaa ccttctgtaa atgctggcgt tcccaccgcg
    25981 ccgtctgaac ctttatgtat actgccgata tagcgtgcgc ccgcgccaac agagatatca
    26041 tcggttgcct gatattggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat
    26101 gaggaaccat cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag
    26161 ccgccaatca cctgccacgc gggagtgata ttccctgcca cggatatctc atagccttcg
    26221 acgcgtttct taccgtattg cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg
    26281 atatcagtac ggaacagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg
    26341 gtgccaatct cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta
    26401 ccactgccag actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca
    26461 tagttaatat agacattgcc gttttccgtc aggtggtaca gcgccccggc tttccagttc
    26521 accagattgc ccgacttggc ggtgtcgacg gtggtgactg gagaaccttt tgccacacca
    26581 gctgggcagg tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca
    26641 gtatgataat tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc
    26701 gtatcgaagg cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc
    26761 gtcaggccgc cgggatgaat tctgctgtca ggatgataaa tatttaccgc cggtaacgtc
    26821 accggattaa cgccatagtt agtctgcgtt tcacgggtaa attccacgcc ggtactgaca
    26881 tcatgaccga tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt
    26941 ttattactca catctttggt attcgccgtg cgtgactagg tccagctatt cacatcgctg
    27001 gtgggctgag taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt
    27061 acgcgcgacc aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa
    27121 cgcatggtgg cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta
    27181 tgagtatcaa cttttccgga atgattcagg gccgccgctc ccgcagatgg ggcagaatag
    27241 cccggcaaac cgatggtcgg aatgccgccg tctggcgtgt tgtgctgagt gacatgcaga
    27301 taattaagat acaaacgatt tgctgtacca aggccaaaag cgacagaagg ggcgacgccg
    27361 taacgctcat ttttgacttt gtcgcgtccg gcatcgtggg ttttttcgcc catcacattc
    27421 aggcgcaccg cggtagtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac
    27481 caggcgctgc caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg
    27541 atcatattga tcgagcctgt cggtgcgctg cgcccgtagt cggtgccgga cggcccttta
    27601 atcacttcga cctgctcggt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaata
    27661 ccatcaatat aaatactgtt agaggtatcg gcaccacgca tataaatggc gtcgccagtg
    27721 gtggagttac cgttctcacc cgcaaaaaac gcacccacgc
//
LOCUS       DAZOZX010000044        27601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZOZX010000044 DAZOZX010000000
VERSION     DAZOZX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.044"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUPT_001826"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUPT_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0819421.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHUPT_001827"
     CDS             complement(1614..1937)
                     /locus_tag="ACHUPT_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0819422.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUPT_001828"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUPT_001828"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0819423.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUPT_001829"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUPT_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0819424.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUPT_001830"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUPT_001830"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0819425.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUPT_001831"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUPT_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0819426.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHUPT_001832"
     CDS             7537..8574
                     /locus_tag="ACHUPT_001832"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0819427.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUPT_001833"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUPT_001833"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0819428.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUPT_001834"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUPT_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0819429.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUPT_001835"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUPT_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0819430.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHUPT_001836"
     CDS             10745..11626
                     /locus_tag="ACHUPT_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0819431.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHUPT_001837"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHUPT_001837"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0819432.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHUPT_001838"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHUPT_001838"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0819433.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHUPT_001839"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHUPT_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHUPT_001840"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHUPT_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0819434.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHUPT_001841"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHUPT_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0819435.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHUPT_001842"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHUPT_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0819436.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHUPT_001843"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHUPT_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0819437.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHUPT_001844"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHUPT_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0819438.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHUPT_001845"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHUPT_001845"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0819439.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHUPT_001846"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHUPT_001846"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0819440.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHUPT_001847"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHUPT_001847"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0819441.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHUPT_001848"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHUPT_001848"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0819442.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHUPT_001849"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHUPT_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0819443.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHUPT_001850"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHUPT_001850"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0819444.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHUPT_001851"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHUPT_001851"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0819445.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHUPT_001852"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHUPT_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR0819446.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHUPT_001853"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHUPT_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR0819447.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHUPT_001854"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHUPT_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6806 a         6866 c         7237 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagccactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZOZX010000045        27371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZOZX010000045 DAZOZX010000000
VERSION     DAZOZX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.045"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..576
                     /locus_tag="ACHUPT_001855"
     CDS             <1..576
                     /locus_tag="ACHUPT_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0819448.1"
                     /translation="LAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLC
                     MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLD
                     NLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEK
                     GSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="ACHUPT_001856"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="ACHUPT_001856"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR0819449.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHUPT_001857"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHUPT_001857"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0819450.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHUPT_001858"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHUPT_001858"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0819451.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHUPT_001859"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHUPT_001859"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0819452.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHUPT_001860"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHUPT_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0819453.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="ACHUPT_001861"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="ACHUPT_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0819454.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="ACHUPT_001862"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="ACHUPT_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0819455.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="ACHUPT_001863"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="ACHUPT_001863"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0819456.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="ACHUPT_001864"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="ACHUPT_001864"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0819457.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="ACHUPT_001865"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="ACHUPT_001865"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0819458.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="ACHUPT_001866"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="ACHUPT_001866"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0819459.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHUPT_001867"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHUPT_001867"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0819460.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHUPT_001868"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHUPT_001868"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0819461.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="ACHUPT_001869"
     CDS             13936..14316
                     /locus_tag="ACHUPT_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0819462.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="ACHUPT_001870"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="ACHUPT_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0819463.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHUPT_001871"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHUPT_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0819464.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHUPT_001872"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHUPT_001872"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0819465.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="ACHUPT_001873"
     tRNA            complement(17417..17492)
                     /locus_tag="ACHUPT_001873"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="ACHUPT_001874"
     tRNA            complement(17496..17571)
                     /locus_tag="ACHUPT_001874"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="ACHUPT_001875"
     tRNA            complement(17623..17698)
                     /locus_tag="ACHUPT_001875"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="ACHUPT_001876"
     tRNA            complement(17701..17776)
                     /locus_tag="ACHUPT_001876"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACHUPT_001877"
     tRNA            complement(17912..17987)
                     /locus_tag="ACHUPT_001877"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHUPT_001878"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHUPT_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0819466.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHUPT_001879"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHUPT_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0819467.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHUPT_001880"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHUPT_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0819468.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHUPT_001881"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHUPT_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0819469.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHUPT_001882"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHUPT_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0819470.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHUPT_001883"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHUPT_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0819471.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHUPT_001884"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHUPT_001884"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0819472.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHUPT_001885"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHUPT_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0819473.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHUPT_001886"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHUPT_001886"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0819474.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHUPT_001887"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHUPT_001887"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0819475.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHUPT_001888"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHUPT_001888"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0819476.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6889 a         7255 c         6703 g         6524 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaatgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaaa gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga t
//
LOCUS       DAZOZX010000046        26641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZOZX010000046 DAZOZX010000000
VERSION     DAZOZX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.046"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            229..336
                     /locus_tag="ACHUPT_001889"
     CDS             229..336
                     /locus_tag="ACHUPT_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0819477.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            712..819
                     /locus_tag="ACHUPT_001890"
     CDS             712..819
                     /locus_tag="ACHUPT_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0819478.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="ACHUPT_001891"
     CDS             complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="ACHUPT_001891"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0819479.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="ACHUPT_001892"
     CDS             complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="ACHUPT_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0819480.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="ACHUPT_001893"
     CDS             complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="ACHUPT_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0819481.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4614..4802
                     /gene="bcsR"
                     /locus_tag="ACHUPT_001894"
     CDS             4614..4802
                     /gene="bcsR"
                     /locus_tag="ACHUPT_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0819482.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4814..5566
                     /gene="bcsQ"
                     /locus_tag="ACHUPT_001895"
     CDS             4814..5566
                     /gene="bcsQ"
                     /locus_tag="ACHUPT_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0819483.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5563..8180
                     /gene="bcsA"
                     /locus_tag="ACHUPT_001896"
                     /pseudo
     CDS             5563..8180
                     /gene="bcsA"
                     /locus_tag="ACHUPT_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8191..10530
                     /gene="bcsB"
                     /locus_tag="ACHUPT_001897"
     CDS             8191..10530
                     /gene="bcsB"
                     /locus_tag="ACHUPT_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0819484.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGTPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10537..11643
                     /gene="bcsZ"
                     /locus_tag="ACHUPT_001898"
     CDS             10537..11643
                     /gene="bcsZ"
                     /locus_tag="ACHUPT_001898"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0819485.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11838..12227
                     /locus_tag="ACHUPT_001899"
     CDS             11838..12227
                     /locus_tag="ACHUPT_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR0819486.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            12309..14297
                     /gene="hmsP"
                     /locus_tag="ACHUPT_001900"
     CDS             12309..14297
                     /gene="hmsP"
                     /locus_tag="ACHUPT_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0819487.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14487..15773
                     /gene="dctA"
                     /locus_tag="ACHUPT_001901"
     CDS             14487..15773
                     /gene="dctA"
                     /locus_tag="ACHUPT_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0819488.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15994..17490
                     /locus_tag="ACHUPT_001902"
     CDS             15994..17490
                     /locus_tag="ACHUPT_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0819489.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(17586..18515)
                     /gene="kdgK"
                     /locus_tag="ACHUPT_001903"
     CDS             complement(17586..18515)
                     /gene="kdgK"
                     /locus_tag="ACHUPT_001903"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0819490.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18747..19514
                     /gene="pdeH"
                     /locus_tag="ACHUPT_001904"
     CDS             18747..19514
                     /gene="pdeH"
                     /locus_tag="ACHUPT_001904"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0819491.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            19584..21644
                     /locus_tag="ACHUPT_001905"
     CDS             19584..21644
                     /locus_tag="ACHUPT_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0819492.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21826..23148)
                     /locus_tag="ACHUPT_001906"
     CDS             complement(21826..23148)
                     /locus_tag="ACHUPT_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0819493.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(23559..24572)
                     /gene="yhjD"
                     /locus_tag="ACHUPT_001907"
     CDS             complement(23559..24572)
                     /gene="yhjD"
                     /locus_tag="ACHUPT_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0819494.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(24621..25520)
                     /locus_tag="ACHUPT_001908"
     CDS             complement(24621..25520)
                     /locus_tag="ACHUPT_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0819495.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26040..>26641
                     /locus_tag="ACHUPT_001909"
     CDS             26040..>26641
                     /locus_tag="ACHUPT_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0819496.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6362 a         6685 c         7269 g         6325 t
ORIGIN      
        1 gtgacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg
       61 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac
      181 aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacgattgc acgcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt
      541 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
      601 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggctaat cttgaaccca
      661 aacaactcaa gaatagccgc gaaacgttgg cattacaaca caggcggcta tatgacgctc
      721 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
      781 gccagtatga tcgtgaactg gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc
      841 tgccgtaatg gcaatttgcg cccggatcag gccgcagagg ttaaactctg cggccttttt
      901 cgtgcttact gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatct
      961 tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca
     1021 ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga
     1081 accaccagat cggagatagc caggaagctg ctcggttggt caatgacaat cggtgccccc
     1141 tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg
     1201 ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt
     1261 tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg
     1321 tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc
     1381 ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt
     1441 ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc
     1501 gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa ttcagtctgc
     1561 atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc
     1621 atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa acagatagca gtcgttattt
     1681 gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc
     1741 gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaaatcaat atcgaaatgc
     1801 gaccacagtg gatgcgacat caacccggcg gcttctatat ccgaccagga aagcgaacag
     1861 atgttaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc
     1921 gatttacgtt tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gttcgccgtt
     1981 gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca
     2041 acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg tccggctggc
     2101 cacaaggaga aacttggtcc cgccagggta agtacgttca gccatagcag tatggcaacc
     2161 acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccactaa taaaacaaaa
     2221 atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta
     2281 ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccagg caaccagatg
     2341 tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg
     2401 ctgtaacgcg gaatgggcat cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg
     2461 aagttaagat atcccgccca caacaggcca aacttaacca gaaaatagaa gttccagccg
     2521 gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga
     2581 gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat
     2641 aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg
     2701 ccagatagcc cagcgggaaa aatatcagtg cgcaaacgat aataatttca atgatatcgc
     2761 tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcatgg gttctggtat
     2821 tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa
     2881 aggcagcggc attccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact
     2941 gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg
     3001 gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac
     3061 cagccgatta ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag
     3121 ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg
     3181 ggcaggcaag agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt
     3241 ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg
     3301 tacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac aacgggagag
     3361 tggcgcattc cacggaataa ccatatttgc accgcaggcc aataacaaac gttcatcggt
     3421 ggcgcgcagg ctagcggtat tttcccgcac gaggattttc atcgcactac cgcgctggcg
     3481 acgcagggta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa
     3541 gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa
     3601 cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat
     3661 gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta atgtccagat
     3721 accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca
     3781 gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag
     3841 tgaacggtat tcctcaagca acaatgaaaa ttgtttatcg ttattatttc cgggattaat
     3901 taccaaaagc gaacaatggt ttaacctgct ccatttattc attttatcca accatgagcg
     3961 aagccgctcg gcaggaatat tttgccatgc gttatttgcg caaacaagaa taaaaaggta
     4021 attatgggga tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg
     4081 atttagcatt gaaaataatt ttattttttc cggcccttga gaatcatcta attgaaagat
     4141 tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc
     4201 aattgtttga ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac
     4261 gccgcctgct ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac
     4321 agggtccaca atgtccctca tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc
     4381 taagaaataa acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta
     4441 gaaggtcaca agacgtgtga ttttagtctt ttcgttttta actttttctg ttgcgtaacg
     4501 gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac aataaaccgg cgaaatgctc
     4561 acggttagaa ttgtctcatg aatggtacgg ttatttcata gggatcaagc aaaatgaata
     4621 acaatgaacc agatactctg cctgatcccg cgataggcta tatcttccag aatgatattg
     4681 tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg
     4741 aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt
     4801 aggggattgg tgaatggccg tactgggatt gcagggggtg cggggaggcg tggggacaac
     4861 aaccatcacc gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt
     4921 cgatgcctgc ccggacaact tgttgcgcct gtcatttaat gttgatttta cccaccgtca
     4981 gggctgggcc agagcgatgc tggatggcca ggactggcgt gacgctgggt tgcgctacac
     5041 ctcgcagctc gatttgctgc cttttggtca gttatccatt gaagaacaag aaaatccaca
     5101 gcactggcaa acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg
     5161 gcgttaccag tggattttaa tcgacttacc gcgtgatgcc tcgcagataa cccaccagct
     5221 gctgagtttg tgcgagcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg
     5281 actgcatcag caagcgctgc cggatggcgc acatattttg attaatgact tccgtattgg
     5341 cagtcaggtt caggacgata tttaccagct ttggttgcaa agccagcgcc gattactgcc
     5401 gatgctcatt catcgtgatg aagcgatggc tgaatgcctg gcggctaagc aaccagtagg
     5461 tgaatatcgc agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct
     5521 gttgaactac tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg
     5581 tggttgctta tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt
     5641 cacggtgcgt cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt
     5701 tttattccgc tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat
     5761 acgcatatca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     5821 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     5881 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     5941 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     6001 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     6061 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     6121 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     6181 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     6241 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     6301 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     6361 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     6421 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     6481 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     6541 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     6601 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     6661 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     6721 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     6781 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     6841 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     6901 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     6961 gtggctatac ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     7021 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtataa atcttccgtc
     7081 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     7141 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     7201 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     7261 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     7321 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     7381 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     7441 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     7501 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     7561 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     7621 cggtatcggt agaaagcaaa caggtacgcc gatcgaaccg cgtggagatg acgatgcccg
     7681 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     7741 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     7801 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     7861 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     7921 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     7981 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     8041 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     8101 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     8161 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     8221 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     8281 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     8341 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     8401 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     8461 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     8521 gcgatgctca acttcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     8581 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     8641 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     8701 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     8761 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     8821 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     8881 ttgccgatgg tctttgcggg tacgccggat gttgggctgc aacaagcatc tgccattgtc
     8941 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     9001 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     9061 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     9121 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     9181 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     9241 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     9301 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     9361 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     9421 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     9481 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     9541 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     9601 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     9661 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     9721 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat cccgatgccg
     9781 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     9841 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     9901 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     9961 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
    10021 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
    10081 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
    10141 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
    10201 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
    10261 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
    10321 atccgcgagt ccggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
    10381 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
    10441 gcgactatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
    10501 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
    10561 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
    10621 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
    10681 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
    10741 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
    10801 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
    10861 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
    10921 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
    10981 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
    11041 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
    11101 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
    11161 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
    11221 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
    11281 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
    11341 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
    11401 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
    11461 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
    11521 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
    11581 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
    11641 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
    11701 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
    11761 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
    11821 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
    11881 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
    11941 actggcgctg ggccccggca gcgtatggat tacttaccga ctggcaggaa gaagctgcgc
    12001 ggcaatccaa cgatggcggc agcagtcagg gcttcggcta cacggcgcgg gcattacttg
    12061 aacgacgtgt tacttccaac tggtttgttg gcacggcaat tgatatccag caggtgaaag
    12121 attacgcacc cagccatttc ctgctctacg tacgttattc cgccgccgga tggcagggtg
    12181 acatggattt accgccgcag ccgctgatac cttacgccga ctggtaagtt ttcagatagc
    12241 gcctctctta atgccgctgc gatcgggtat actcgggcgg caatctggga tttccggggg
    12301 gagacaattt gcgcgtaagt cgctcgttaa caatcaagca gatggcaatg gtggcagccg
    12361 ttgtcctggt gttcgttttt attttttgca ccgttttgct gttccatctg gtccagcaga
    12421 atcgctataa cacggctacg caactggaaa gcattgctcg ctctgtccgc gaacccttat
    12481 cttcagctat tttgaaaggc gatattcccg aagcggaagc tattcttgcc agcattaaac
    12541 cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa ccagttccag gcgctgcgta
    12601 aaagttttat tccagagcgt ccggtgccgg taatggttac tcgcctgttt gagctaccgg
    12661 ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc aaatccgcag ccaatagcct
    12721 atctggtgct acaggcggat tccttccgta tgtataagtt cgtgatgagc actctctcaa
    12781 cgttagtgac catttactta cttttgtcgc ttatcctgac cgtcgccatc agctggtgca
    12841 ttaaccgcct gattttgcat ccgttacgca atattgctcg cgaacttaac gccatcccag
    12901 cccaggagct tgttggtcac caactggcat taccgcgtct gcatcaggac gatgaaatcg
    12961 gtatgttggt gcgcagttac aacctcaacc agcaattgct gcagcgccat tatgaagaac
    13021 agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg
    13081 agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg
    13141 aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca acgagaaatt ctgctgctga
    13201 cgctggtgga aaaactcaaa tcggtactgt cgccacgtat gatcctcgcg cagattagcg
    13261 gttatgactt tgctgtcatt gccaacggtg tacaggaacc gtggcacgca atcactttgg
    13321 gtcagcaagt actcactatc atgagcgagc gcctgccgat tgaacgtatt caactccgtc
    13381 cgcactgtag cattggcgtg gcgatgttct acggcgatct caccgccgaa cagctttaca
    13441 gtcgcgctat ttctgcggca tttaccgctc gccataaagg caagaatcag attcagttct
    13501 ttgatccgca gcagatggaa gcagcccaga agcggttgac ggaagagagc gatatcctta
    13561 atgcactgga aaatcatcag tttgcaattt ggttacagcc acaggtcgag atgaccagcg
    13621 gtaaactggt cagtgcggaa gtgttactgc gtatccagca accggatggc agttgggacc
    13681 tgccggatgg cttaatcgat cgcattgagt gctgtgggct gatggttacc gtcggtcact
    13741 gggtgctgga agagtcctgt cgattgcttg cagcctggca agagcgcggc attatgctgc
    13801 ccttgtcggt aaacctctct gcgctgcaac tgatgcaccc gaatatggtg gcggatatgc
    13861 tggaactgtt aacccgctat cgcattcagc cgggaacact gattctggaa gtgacagaaa
    13921 gccgacgtat tgacgaccct catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg
    13981 gagttcgggt ggcgctggat gatttcggca tgggctacgc agggctgcgt cagctgcagc
    14041 atatgaaatc gttgccaatc gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc
    14101 cggaagatag cagcatgatt gctgcaatta tcatgctggc gcagagcctg aacttacaaa
    14161 tgattgccga aggcgtggag actgaagcac aacgagactg gctggcaaaa gcgggcgttg
    14221 gtattgccca gggcttcctt tttgctcgcc cactccctat tgaaatcttc gaagagagtt
    14281 acctggaaga aaagtagcta ccccaaactg attacaaaac tttaaaaagt gctggtttgt
    14341 gcgagccagc tcaaactttt taaccttttt gtttcaatta tgatccaggt acatttctgt
    14401 gatgttgtct gggtgttatt ttaaggccgc aggtaccccc ccccccataa ccttacaaga
    14461 cctgtggttt tactaaagga caccctatga aaacctctct gtttaaaagc ctttactttc
    14521 aggtcctgac agcgatagcc attggtattc tccttggcca tttctatcct gaaataggcg
    14581 agcaaatgaa accgcttggc gacggcttcg ttaagctcat taagatgatc atcgctcctg
    14641 tcatcttttg taccgtcgta acgggcattg cgggcatgga aagcatgaag gcggtcggtc
    14701 gtaccggcgc agtcgcactg ctttactttg aaattgtcag taccatcgcg ctgattattg
    14761 gtcttatcat cgttaacgtc gtgcagcctg gtgccggaat gaacgtcgat ccggcaacgc
    14821 ttgatgcgaa agcggtagcg gtttacgccg atcaggcgaa agaccagggc attgtcgcct
    14881 tcattatgga tgtcatcccg gcgagcgtca ttggcgcatt tgccagcggt aacattctgc
    14941 aggtgctgct gtttgccgta ctgtttggtt ttgcgctcca ccgtctgggc agcaaaggcc
    15001 aactgatttt taacgtgatc gaaagtttct cgcaggtcat cttcggcatc atcaatatga
    15061 tcatgcgtct ggcacctatt ggtgcgctcg gggcaatggc gtttaccatc ggtaaatacg
    15121 gcgtcggcac actggtgcaa ctggggcagc tgattatctg tttctacatt acctgtatcc
    15181 tgtttgtggt gctggtattg ggttcaatcg ctaaagcgac tggtttcagt atcttcaaat
    15241 ttatccgcta catccgtgaa gaactgctga ttgtactggg gacttcatct tccgagtcgg
    15301 cgctgccgcg tatgctcgac aagatggaga aactcggctg ccgtaaatcg gtggtggggc
    15361 tggtcatccc gacaggctac tcgtttaacc ttgatggcac atcgatatac ctgacaatgg
    15421 cggcggtgtt tatcgcccag gccactaaca gtcagatgga tatcgtccac caaatcacgc
    15481 tgttaatcgt gttgctgctt tcttctaaag gggcggcagg ggtaacgggt agtggcttta
    15541 tcgtgctggc ggcgacgctc tctgcggtgg gccatttgcc ggtagcgggt ctggcgctga
    15601 tcctcggtat cgaccgcttt atgtcagaag ctcgtgcgct aactaacctg gtcggtaacg
    15661 gcgtagcgac cattgtcgtt gctaagtggg tgaaagaact ggaccacaaa aaactggacg
    15721 atgtgctgaa taatcgtgcg ccggatggca aaacgcacga attatcctct taatctcacc
    15781 acttatgccc gcactccctt ccccgagtgc gggtgtttgc gcataattga cccctgtgcc
    15841 tgaacttacc acctgggtga gttcaggttt atttcacttc ttccgtgaca ttttcatgtt
    15901 cttgcggtct aacacgaagt gtttttaacg tcatttagac tgaccgacaa cgggcagttt
    15961 ttttattacc aggatagttg atcaggggtt cacatgcagg gcataaaaat tcgactttta
    16021 gcgggcggtt tgctgatgat ggccactgct ggctatgtgc aggcagatgc gctccagcct
    16081 gatccagcat ggcaacaggg gacgctttcc aacggtttac agtggcaagt gctgaccacc
    16141 ccccagcgtc ccagcgatcg tgttgaaatt cgcctgctgg ttaataccgg ttcgctcgcc
    16201 gaaagtacac aacagagcgg ttacagtcac gccatccctc gtattgcgct aacgcaaagc
    16261 ggtggccttg acgcagcaca ggcgcgttca ttgtggcagc aggggatcga ccctaaacgc
    16321 ccgatgccgc cggtaattgt ctcttatgac accacgctgt ttaatctgag tttgcccaat
    16381 aaccgtaacg atttgctgaa agaagcgctc tcttatctgg caaatgccac tggcaaattg
    16441 accatcacac cagaaaccat caaccacgcg ctgcaaagtc aggacatggt ggcaacctgg
    16501 cctgccgata ctaaagaggg ctggtggcgc tatcgtctga aagggtcaac cttgttaggt
    16561 cacgatcctg ccgatccgct gaaacaaccc gttgaagcgg aaaaaattaa agatttctat
    16621 cagaaatggt acaccccgga tgcaatgacg ctactggtgg tgggaaacgt ggatgcgcgc
    16681 tcggttgtcg accaaatcaa caaaacgttt ggcgaactga aaggcaaacg tgaaacgcca
    16741 gctccggtgc cgacgctttc tccgctgcgt gcggaagcgg tgagtattat gactgacgcg
    16801 gtgcgtcagg accggttatc tatcatgtgg gatacgccgt ggcagccgat tcgtgaatca
    16861 gccgcactgc tgcgctactg gcgtgcggac ctggcccgcg aggcgctgtt ctggcatgtt
    16921 cagcaagcgt taagcgccag tgacagcaaa gacatcggtc ttggatttga ctgccgtgtg
    16981 ctgtatctgc gtgcgcagtg tgccatcaac atcgaatcac caaacgacaa gctgaacagc
    17041 aaccttaatc tggtggcgcg tgaactggcg aaggttcgcg ataaaggtct gccggaagaa
    17101 gagttcaatg cgttagtggc gcaaaagaaa ctggagctgc agaaactgtt tgccgcctat
    17161 gcacgggctg ataccgatat tctgatgggc cagcggatgc gttcgttgca aaatcaggtc
    17221 gtcgatatcg cgccggagca gtatcagaaa ctgcggcagg atttccttaa cagcctgact
    17281 gtggagatgt taaatcagga tctgcgtcag cagttgtcga atgatatggc gttaatactg
    17341 ctgcagccga aaggcgagcc ggaatttaac atgaaagcgt tgcaggcggc ctgggatcaa
    17401 atcatggccc catctaccgc cgctgcgacc acctctgtcg ccacggatga cgtacatcct
    17461 gaagtgacgg atattccacc tgcacagtaa tgcctgatgg tgatgagtta gcacaaccat
    17521 caggtattta tggatgagct ggatagaggg gtaacacttt atccagcctt ttgcatatgc
    17581 tgcgtttacg ctggcatcgc ctcacgcggg ataatcgcgc cgcgatactg aataacggta
    17641 cttgcggtca ggtgcccgcg tttcgccgcg ttttccgcac tgccgcccgt caaacgtacc
    17701 gccagataac cggcgctgaa agagtcgcca gctgcggtgg tatcgatcac tttttctttc
    17761 ggcagtttca ccgccggaac atcaactaac gcttcaccag caatggacac caggcaagaa
    17821 tccgccccgc gtttcaccac cacttctttc acgcccgcgt tatgggtgcg cgcaatgacg
    17881 tcttccaccg gctgttgacc ccacagcgcg tcttcgtcgt ccagcgtcag gaaggcgata
    17941 tccgtgcatt ccagcatttg ctggtacacc tgctgtgtct cttctttgct ggcccacagg
    18001 cgcggacgat agttattgtc gaaaatcact tttccgcctt tggcgcggca ttcgcgcagc
    18061 agggaaagca gcttttcgcg gctggtcggg cttaagatcg ccaggctaat cccgctcagg
    18121 tagagataat cgaaattcgc cagctcttcg caaatcgccg cagactgctc gctcgccagc
    18181 cagaatttgg cggcggcttc gttacgccag tagtagaacg tacgttcgcc ggtgctgtcg
    18241 gtttcaatgt agtaaaggcc cggaagacgg ttttccatcc gttgggtcag ggaagtatca
    18301 acgttctcgc cgtgccaggc gtccagcatc tgctggctaa aactgtccgt tcccagcgcc
    18361 gttacgtaat gaacggttaa tgccgcagga tcgacctgac gggcgatata gacggaagtg
    18421 ttcagggtat cgccgccgaa accgcgctta acgtccgcgc ctttctcgga aagctcaatc
    18481 atgcattcgc caatcacggc aatctttttg gacatagtcg tgaacctgat ctgtaaaaaa
    18541 ttagcgttag tttgcgctgt ggtggtttgc tggtcaacca tattaaaaca gtgttccatt
    18601 atttttttga gccagaacgt attcctgaaa gattccgttg tggagtgagg aaatttaccc
    18661 cggccaggcg gtaaagttct gcccttacgc gccgataatc tttgtcgagt ccgggcagca
    18721 tcacttttaa acacaggaca tctttgatga taaggcaggt tatccagcga ataagcaacc
    18781 ctgaagcaag catcgagagc ttgcaggaac ggcgtttttg gttgcagtgt gagcgtgctt
    18841 acacctggca gccgatctat caaacatgcg ggcggttaat ggccgtggag ctattaacgg
    18901 tggtcacgca tcccttgaac ccttcgcaac gcctgccgcc ggatcgctat tttactgaaa
    18961 tcaccgtcag ccatcggatg gaggttgtga aagagcagat tgatttgctg gcgcaaaaag
    19021 ccgacttctt tgtagagcac ggcctgctgg catcggtcaa tattgatggc cctacgctca
    19081 tcgccctgcg tcagcaacca aaaatcctgc gccagattga gcgtcttccc tggctgcgtt
    19141 tcgaactggt ggagcatatc cgtctgccga aagattcaac ctttgcctcg atgtgtgaat
    19201 ttggcccgct gtggctggat gattttggta ccgggatggc aaatttctct gcactaagtg
    19261 aagtgcgtta tgactacatc aaaatcgcgc gagaactgtt tgtgatgctg cgtcagtcgc
    19321 cggaaggccg cacactcttt tctcagcttt tacatctaat gaatcgctat tgtcgcgggg
    19381 tgattgtcga gggcgtagaa acaccggaag agtggcgtga tgttcagaac tcgcccgcat
    19441 tcgccgcaca aggctggttt ctttcacgcc cggcaccgat ggaaacgctg aatacggcgg
    19501 ttctggcgct ataagctgcc tcattttccg ccgggctgga ctatctttag gactggcaca
    19561 ggaagaatat gaggaagcga acgatgagca aggcaggcaa aataaccgct gcgatttcag
    19621 gggctttctt gttgttgatt gtcgtggcga tcattttgat tgcaactttt gactggaatc
    19681 gactcaaacc gaccatcaac cagaaagtct ctgcggagtt gaatcgtccg ttcgctatcc
    19741 gtggcaatct gggcgtggtg tgggagcggc aaaaacaaga aactggctgg cgcagctggg
    19801 tgccgtggcc tcatgtacat gcagaagaca tcattcttgg taatccaccg gatattcccg
    19861 aagtcacgat ggtgcatttg ccgcgagtag aggcaacgct ggcaccgctg gcgctgctga
    19921 ccaaaacggt ctggctgccg tggatcaagc tcgaaaagcc cgacgcgcgc ctgattcgcc
    19981 tctctgaaaa gaacaataac tggacgttta atcttgccaa cgatgacaac aaagacgcga
    20041 atgcaaagcc gtcggcatgg tcgtttcggc tggataatat tcttttcgat caagggcgga
    20101 tcgccattga tgacaaagta agcaaagcgg atctggaaat ttttgttgat ccgttaggca
    20161 agccgctgcc attcagcgaa gttactggat cgaaaggtaa agcggataaa gaaaaggtgg
    20221 gcgattacat ttttggcctg aaggcgcagg gacgttataa cggtgaaccg ctcacgggta
    20281 cgggaaaaat aggcggtatg ctggcgctgc gtggcgaagg gacgccgttt ccggtacagg
    20341 ctgatttccg ttcaggtaat acccgtgttg cttttgatgg cgtcgtgaat gacccaatga
    20401 agatgggcgg tgtcgattta cggcttaaat tttctggcga ttcactgggt gatctctatg
    20461 aactgacggg cgttctgctg cccgataccc cgccgtttga aacggatggt cggctggtag
    20521 cgaaaatcga cactgaaaaa tcgtcggtct ttgattatcg cggttttaat gggcgaattg
    20581 gcgatagcga tatccacggt tctctggtct acaccaccgg aaagccacga ccaaaactgg
    20641 aaggtgatgt cgagtcgcgg caattgcggc tggcggacct gggaccgttg attggcgttg
    20701 attccgggaa aggggcagaa aagtcgaaac ggtctgaaca gaagaagggc gaaaaaagcg
    20761 ttcagcctgc gggcaaagtg ctgccttatg accgcttcga aaccgataaa tgggacgtta
    20821 tggatgccga tgttcgcttc aaagggcggc gcattgagca tggcagtagc ctgccgatta
    20881 gcgatctttc tactcatatc atcctcaaaa atgctgacct gcgcctgcaa ccgctgaaat
    20941 ttggcatggc gggcggcagc attgcggcga atattcatct ggaaggcgat aaaaagccga
    21001 tgcaggggcg ggcagatatt caggctcgtc gactgaaact gaaagaactg atgcccgatg
    21061 tggaactgat gcagaagacg ctgggggaaa tgaacggtga cgcggaacta cgcggtagcg
    21121 gtaactcggt ggcggcgctt ttaggcaaca gtaacggcaa cctgaaactg ttgatgaatg
    21181 acgggctggt gagccgcaac ctgatggaga ttgttgggct gaatgtcggc aactacattg
    21241 tcggtgcgat atttggtgac gatgaggtgc gggtgaactg cgcggcggcg aatctgaata
    21301 ttgccaacgg cgtggcgcgc ccgcagattt ttgctttcga tactgagaac gcgttgatta
    21361 atgttaccgg cacggcaagt tttgcttcgg aacagctgga tttgactatt gatccggaga
    21421 gtaaagggat tcggattatc acactgcgtt cgccgctgta tgtgcgtggg acgtttaaaa
    21481 atccgcaggc tggggtgaaa gccggaccgc tgattgcccg tggtgctgtt gctgcggcac
    21541 tggcaacgct ggtaacaccg gcggcggcgt tactggcact gatctcacct tccgaagggg
    21601 aggctaatca gtgtcggacg atattgtcgc agatgaagaa gtgatgataa ttgccggatg
    21661 tctgacatcc ggcaattaaa cagatttaga tttttggcaa agtgtgcttt gttcatgccg
    21721 gatgcggcgt gaacgcctta tccggcctac aaatccttgt gaattcaata tattgtatgg
    21781 aaattcagtc ctgataagcg tagcgcatca ggcttttcac acttattaca acgactgatg
    21841 tcgcgtctca tgggtcagca gcagggcgat taacgtcaag ccagccatcg ccgccagata
    21901 taaccccacc gcacctaacc cgtagttagc ctgcaaccag gctgcgatat acggcgcaac
    21961 ggaagccccg agaatcgacg atacgttgta agagaacgat gctccggtgt aacgcacttc
    22021 tgtcggaaac agctctggta acagcgcacc catcggcccg aaggtcagcc ccatcagact
    22081 taaccccagc agcaggaagg caaaaaccag aatcgggttg ccagaaccga gcagcgggtt
    22141 aaaggcgaac agcgcgaaca ggatgatcag cgtggtgatg atcaccatgc ttttacggcg
    22201 accaaaggca tcagccagta atccggcgac tggcaccatc acgccaaaac caataactgc
    22261 catcatcagc atccacaaca cttcgttacg cggcaggcca agcccaactg gcgcggcggc
    22321 ggtactgaag gtcatagagt agaccgtcat gatgtaaaac agcgtatagg ttgccagcat
    22381 aatgaacgta cccagtacgg ttacgcgaac atgtttggtc agcagcgtac ccagcgggat
    22441 cttcacctgt tttttcgctt tagcgacttt ctcaaacacc ggcgactcat gcagcgacac
    22501 gcgaacatac aggccgataa tgaccagcac cgccgagaag ataaatggca cacgccagcc
    22561 ccagctcata aactgctcat cggtcagtag ccaggaaagc agcaagaaag tgccgttggc
    22621 aaagaagaag ccaatcggtg cgcctagctg tgggaaggag ccatacagtg cacgtttgcg
    22681 cggtggggcg ttttcagtcg ccagcagcgc cgcgccgccc cattcaccgc ctaagcccag
    22741 accctgacca aatcgagcca gcgccagcag cagtggggcg aaaataccaa tcgtggcata
    22801 gcccggcagc agaccaatca ccacggtcga aatccccatc gttagcaacg aggcgaccag
    22861 cgtcgcttta cgcccaacgc gatcgccaaa atgaccaaaa acggcagagc caatggggcg
    22921 cgcgacgaag gcgatggcga aggtggcgag cgactgtagc gttgctgccg ttggatcgcc
    22981 ctgcgggaag aagatatgcg gaaacacaat aacggccgca gtggcgtaaa tgtaaaagtc
    23041 gaagaactca atggccgtgc caatgagaga ggcgacaagg actttattac gcgagttgat
    23101 cggcgtgtat tcttgttcgt ggtcgagtgt tgtggctgtt gcttgcataa tcttttctta
    23161 ttttttggct aacgaatagc catattacgc acagcaaaag tgatatttca atgtgtaaca
    23221 aatcgcggaa atggtcaaaa accaggcaaa aaggggataa tgtttaggcc agtgattttt
    23281 cttctatgaa atgcttcaca taattttata aatagtggat aaaactggtt tcaggttaaa
    23341 taaaagttac caactggatt tatatgctgg ggttatgagt tggagtgaaa ttgtttgctc
    23401 ccaaatagat ggctgaagag gctctgggga agagtttcaa gaggttatgt gcgcattata
    23461 gccggatgcg atgctggcgc atcttatccg gcctacgggg agccaggccg aataaaatcg
    23521 tatgccggat aaggcgttta cgccgcatcc ggcctatttt aaggctgcgt tttccctggc
    23581 attcgcgggt cgtctttata ttcggcggtg gcaatccacg ccgcgcaaaa cagcgtcaaa
    23641 cgggcgaaga agtagaaaaa tgccatcagc cccagcacgg aaccaaatgc tgcgccagag
    23701 ggggatttca tcagcgatgg cagggtgtag gtcatcacga ttttaatcac ttcaaaacca
    23761 atagcagcca gaaatgttcc gcgaatcagg gcttttttgc gcggacggtg gcgcggcaac
    23821 cgccagaaga tccagaagaa aagcagatag ttggcgaaga tggaaatcgc caggccaatc
    23881 aatcgccacg tcggtttcag ccactcaatg ctgttcaggt gcagggcgct aataatcatt
    23941 tgctgcgccg aaccggcaac cgaggtgatc gaaagcgtca caatcagcgc aatcaacaaa
    24001 ccaatcagtg aaataaaatc acgcagatat tttacccaga acttctcctg atcttgcggc
    24061 gagcgttccc agacatcgcg cgactgggca cgaatcgctt cacgcaggtt acccatccag
    24121 ttgatgccgg aataaagtgc caccgccagg ccgacaagcc ctacagtcgt acgctgctga
    24181 acggcggtgt tgatggtgtt tttcaacgtg gcggctagtg tcggatcgct gatgttttgc
    24241 agaattttgt cgaagatatc ctgtagcaac atcggatggg aggccagcac aaagccccct
    24301 gcggcaaacg acaccatcaa aatcgggatc atcgacaaaa atgagaaata ggtgatagcc
    24361 gcaccaaact ggttacccag ccgatcgtta aagcgttctg tcgcgcgaat aaggtgcgca
    24421 ataaccggtt ggcgttggac tttttcggca gtggtggtga cggtttccag cgcctggcta
    24481 actttgccgc ctttctcgct attatccaac tgcttaatcg gctcgtgctc cagatcctgg
    24541 gtgggacgtt tgatctcgtt ttcctgcgtc atccgctctt ttgtccttct gtcgtggtca
    24601 ttcttaaaaa gtatagtcgg tcagtccagg tgctctttca ttacgccagc cagccactgc
    24661 ataaacaggt ttacacgccg ggaaagctcc cgacgctgcg ggtaaaccag cgaaagggag
    24721 agcggctcgg cacggtagcc tggcaacact tcaataagcc gcccggcacg cagggcttcg
    24781 cgcacggcaa tgcgcgggat ctgaataatc cccagaccgg caagaccggc ggcgagatag
    24841 ttttcgctac tgtttaccgt caacatgccg ccagacttaa accactggac gccattaacg
    24901 ctggcaacct caaaacctaa cggatgtaca cccaggtgcg gtgtataacg cactattgcg
    24961 tgtgaagtca gatcatcggg gctttgcgga taaccaaagc gcgtcagata gtgcggactg
    25021 gcacagttga ccatggttag tttgccgagg ggacgggcga taacgccatc ttccggtaac
    25081 gcgccagtgc gtatcacgca atcaaaacca tcatgaagaa tatctaccgg acggttatgg
    25141 ctactcagtt ccagctcaat tcccggatgc tgatagagaa attccgacag acgcggcagt
    25201 aacaggtttt tcgcgattcc tgacgggatg tcgatgcgta atttaccgct gatactggtg
    25261 gcatcctgtt gaaacagacc gtccagttcg ctgagattac tcaacacatc ttttgcccgt
    25321 tgataatagg tcatgccttc tggcgtgagt ttgacccgtc gcgtagtgcg ctggagaagc
    25381 tgggtgccaa gttgatgttc cagtgcctgt atctggcgcg aaacacttcc ctttggcaaa
    25441 gcaaagaaat ccgccgcgcg ggaaaaactt tccagctccg cgactttgat gaacaactgc
    25501 attgcgtgaa ttttatccat ttctttgacc gattgttgtt cacagcgaaa cagtaacgcg
    25561 catatcgcat tgtttattgt tttttctaac atctaatgcc gggtttcaca atgttgtcat
    25621 gacgaatgtg gcgatttctc agcaggaaaa gggcggaata gctttatgag aaaggtgaat
    25681 ccccttaaac agagcgtatt tttcagtgat tccctttaaa gtgaatagag gacgtattgg
    25741 ctatttgtgc ttatttttgt ctcattgcca tttgttattt tggcttgtag tcgcggaatt
    25801 tcatttttaa ttgcatgcgt actaaccacc aatataagga aatcctcaaa attgttttaa
    25861 atagtgccct cgacatgtaa tttcaggcgt ttagctgtaa ctatttatat cggtaactat
    25921 tttctacgcg tcatctctgt agaatggcca ccgccaaatg gcgaattctt attttttctc
    25981 gcgggtaata cccttccact acttaagtct ttttaacgta ttcaggtgga gattcagtta
    26041 tgcaaatagt catgtttgac aggcagtcaa tatttattca tggaataaaa atcagtttac
    26101 agcagcgtat tccaggagtg agtattcagg gggtcagtca ggcagacgag ttatggcaaa
    26161 agctggaaag ttaccctgaa gccttagtta tgctcgatgg cgatcaggat ggtgagtttt
    26221 gctactggtt gctgcaaaaa accgtggtgc agtttcctga ggttaaggtg ttaatcacgg
    26281 cgacggattg caataaacgg tggttacagg aagttatcca ttttaatgtg ctggccattg
    26341 tgcctcgcga ttcaaccgtc gagacatttg cgttggcggt aaatagtgcg gcgatgggga
    26401 tgatgtttct gcctggagac tggcggacga cgccggaaaa ggacattaaa gatctcaaat
    26461 cgttaagcgc ccgccagcgg gagattttaa ccatgttagc ggcgggcgaa tcaaataagg
    26521 agatcggcag agcgctgaat atcagtaccg gaacggtaaa agcgcatctg gaatccctgt
    26581 atcgccgtct ggaagtgaaa aatcgcaccc aggcggcaat gatgttaaat atctcctcct
    26641 g
//
LOCUS       DAZOZX010000047        26186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZOZX010000047 DAZOZX010000000
VERSION     DAZOZX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.047"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUPT_001910"
     CDS             complement(<1..215)
                     /locus_tag="ACHUPT_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR0819497.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
     gene            525..2297
                     /gene="aspS"
                     /locus_tag="ACHUPT_001911"
     CDS             525..2297
                     /gene="aspS"
                     /locus_tag="ACHUPT_001911"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0819498.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2415..2867
                     /gene="nudB"
                     /locus_tag="ACHUPT_001912"
     CDS             2415..2867
                     /gene="nudB"
                     /locus_tag="ACHUPT_001912"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0819499.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2896..3636
                     /locus_tag="ACHUPT_001913"
     CDS             2896..3636
                     /locus_tag="ACHUPT_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0819500.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3671..4192
                     /gene="ruvC"
                     /locus_tag="ACHUPT_001914"
     CDS             3671..4192
                     /gene="ruvC"
                     /locus_tag="ACHUPT_001914"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0819501.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4194..4796)
                     /locus_tag="ACHUPT_001915"
     CDS             complement(4194..4796)
                     /locus_tag="ACHUPT_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0819502.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4866..4931
                     /gene="yobI"
                     /locus_tag="ACHUPT_001916"
     CDS             4866..4931
                     /gene="yobI"
                     /locus_tag="ACHUPT_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR0819503.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5070..5681
                     /gene="ruvA"
                     /locus_tag="ACHUPT_001917"
     CDS             5070..5681
                     /gene="ruvA"
                     /locus_tag="ACHUPT_001917"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0819504.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5690..6700
                     /gene="ruvB"
                     /locus_tag="ACHUPT_001918"
     CDS             5690..6700
                     /gene="ruvB"
                     /locus_tag="ACHUPT_001918"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0819505.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="ACHUPT_001919"
     CDS             complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="ACHUPT_001919"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0819506.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="ACHUPT_001920"
     CDS             complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="ACHUPT_001920"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0819507.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8463..9395
                     /gene="znuA"
                     /locus_tag="ACHUPT_001921"
     CDS             8463..9395
                     /gene="znuA"
                     /locus_tag="ACHUPT_001921"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0819508.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9411..10733
                     /gene="mepM"
                     /locus_tag="ACHUPT_001922"
     CDS             9411..10733
                     /gene="mepM"
                     /locus_tag="ACHUPT_001922"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0819509.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10853..11824
                     /gene="lpxM"
                     /locus_tag="ACHUPT_001923"
                     /gene_synonym="msbB"
     CDS             10853..11824
                     /gene="lpxM"
                     /locus_tag="ACHUPT_001923"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0819510.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="ACHUPT_001924"
     CDS             complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="ACHUPT_001924"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0819511.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="ACHUPT_001925"
                     /gene_synonym="yebK"
     CDS             complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="ACHUPT_001925"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0819512.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14731..16206
                     /gene="zwf"
                     /locus_tag="ACHUPT_001926"
     CDS             14731..16206
                     /gene="zwf"
                     /locus_tag="ACHUPT_001926"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0819513.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16441..18252
                     /gene="edd"
                     /locus_tag="ACHUPT_001927"
     CDS             16441..18252
                     /gene="edd"
                     /locus_tag="ACHUPT_001927"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0819514.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18289..18930
                     /gene="kdgA"
                     /locus_tag="ACHUPT_001928"
                     /gene_synonym="eda"
     CDS             18289..18930
                     /gene="kdgA"
                     /locus_tag="ACHUPT_001928"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0819515.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18986..20164)
                     /gene="purT"
                     /locus_tag="ACHUPT_001929"
     CDS             complement(18986..20164)
                     /gene="purT"
                     /locus_tag="ACHUPT_001929"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0819516.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20298..20588
                     /gene="yebG"
                     /locus_tag="ACHUPT_001930"
                     /pseudo
     CDS             20298..20588
                     /gene="yebG"
                     /locus_tag="ACHUPT_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20655..21011
                     /gene="yebF"
                     /locus_tag="ACHUPT_001931"
     CDS             20655..21011
                     /gene="yebF"
                     /locus_tag="ACHUPT_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0819517.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21338..21997
                     /locus_tag="ACHUPT_001932"
     CDS             21338..21997
                     /locus_tag="ACHUPT_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0819518.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22207..24267
                     /gene="ptrB"
                     /locus_tag="ACHUPT_001933"
     CDS             22207..24267
                     /gene="ptrB"
                     /locus_tag="ACHUPT_001933"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0819519.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24264..24926)
                     /gene="exoX"
                     /locus_tag="ACHUPT_001934"
     CDS             complement(24264..24926)
                     /gene="exoX"
                     /locus_tag="ACHUPT_001934"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0819520.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24950..25606)
                     /gene="yobB"
                     /locus_tag="ACHUPT_001935"
                     /pseudo
     CDS             complement(24950..25606)
                     /gene="yobB"
                     /locus_tag="ACHUPT_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25708..25938)
                     /gene="holE"
                     /locus_tag="ACHUPT_001936"
     CDS             complement(25708..25938)
                     /gene="holE"
                     /locus_tag="ACHUPT_001936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0819521.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26077..>26186
                     /locus_tag="ACHUPT_001937"
     CDS             26077..>26186
                     /locus_tag="ACHUPT_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819522.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAA"
BASE COUNT         6652 a         6486 c         6819 g         6229 t
ORIGIN      
        1 gcatcaaccg gctgttttaa tgcttcggcg taatcggcag accagccaac tcgcaccaga
       61 aacacgggct gaccgctggc gcgaaatttc gccgccagct tcccggcgcg attaactacc
      121 tcatcggcag tatgtggacc accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc
      181 accaacgcag tggttttagc attaagttca agcatgctga ctcctgaaaa aacaaaatcg
      241 tgcctcacac cttaatgcga ttagccgatg gcggaacgac aaattttgtt aatttttatg
      301 agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc ccgatataaa
      361 gtggtaacga ggcgttccag cgcgggccag gctcttacgg tgcggcagaa tttccagtat
      421 aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact tggtcacctg
      481 cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt acagaatatt
      541 gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc
      601 gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc
      661 aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg
      721 agttctgcat tcaggttacg ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg
      781 atatggcgac cggcgaaatt gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag
      841 atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg aaataccgct
      901 acctcgacct gcgtcgcccg gaaatggcgc aacgcctgaa aacccgcgct aaaatcacca
      961 gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact ccgatgctga
     1021 ccaaagccac gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg cacaaaggta
     1081 aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg atgtccggtt
     1141 ttgaccgtta ctatcagatc gttaaatgct tccgtgacga agacctgcgc gctgatcgtc
     1201 agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg
     1261 aagtgatgga agcgctggtg cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg
     1321 atttcccggt aatgaccttt gcggaagcag aacgccgtta tggttctgat aaaccggatc
     1381 tgcgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt gagtttgcag
     1441 tatttgcggg gccagcgaac gatccgaaag gccgtgttgc cgctctacgt gttccgggtg
     1501 gcgcatcgct gacccgtaag cagatcgacg aatacggtaa cttcgttaaa atctacggcg
     1561 cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca
     1621 gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggat cgtactgccg
     1681 cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt gccgacggga
     1741 tgggcgcact gctcctgaaa gtgggtaaag accttggtct gaccgacgaa agcaaatggg
     1801 caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg
     1861 caatgcacca tccgttcacc tcaccgaaag atatgactgc tgcagaactg aaagctgcac
     1921 cggaaaatgc ggtggcgaac gcttacgata tggtcatcaa tggttacgaa gtgggcggtg
     1981 gttcagtacg tatccataat ggtgatatgc agcagacggt gtttggtatt ctgggtatca
     2041 acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa tacggtactc
     2101 cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg actggcaccg
     2161 acaatatccg tgacgttatc gccttcccga aaaccacggc ggcagcgtgt ctgatgactg
     2221 aagcaccgag ctttgctaac ccgactgcac tggctgagct gagcattcag gttgtgaaga
     2281 aggctgagaa taactgatat gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg
     2341 caactgagtg aactcccatg agcatagata actatgtgaa tgggatgagc gaaggcagtc
     2401 aacgaagagg cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca
     2461 tctatgcaca agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat cccgatttct
     2521 ggcagtcggt aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg
     2581 aagtaaagga agaggttacc attgatgttg tcgctgaaca actgacctta attgactgtc
     2641 agcgcacggt agagtttgaa attttttcac atttacgtca tcgctatgcg ccgggcgtga
     2701 cgcgtaatac ggaatcatgg ttctgtcttg cgcttccgca cgagcggcag atcgttttca
     2761 ctgaacatct ggcttacaag tggcttgatg cgcctgctgc ggcggcgctc actaagtcct
     2821 ggagcaaccg gcaggcgatt gaacagtttg taattaacgc tgcctgaaca ggcagggcta
     2881 tctggagata tttttatggc aggtcatagt aaatgggcca acaccagaca tcgtaaagct
     2941 gcgcaggatg ctaagcgcgg taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg
     3001 gctaagctgg gcggtggcga tccggacgct aacccgcgtc tgcgtgcggc ggtggataaa
     3061 gcactgtcta acaacatgac ccgtgacaca ctgaaccgcg caattgcacg tggtgtgggc
     3121 ggtgatgatg atgcaaacat ggaaaccatc atctacgaag gttacggtcc tggcggcacg
     3181 gcaatcatga ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga agtgcgtcac
     3241 gcattcagca aatgtggcgg taacctcggc actgacggtt ccgtagctta tctgttcagc
     3301 aaaaaaggcg tgatctcctt cgagaaaggc gacgaagaca ccatcatgga agcagctctg
     3361 gaagcaggtg ctgaagacgt tgtgacatat gatgacggcg cgattgatgt ctacactgca
     3421 tgggaagaaa tgggtaaagt gcgcgacgct ctggaagcgg caggtctgaa agcagacagc
     3481 gcggaagttt ccatgatccc gtccaccaaa gctgatatgg atgcagaaac cgcgccgaaa
     3541 ctgatgcgtc tgatcgatat gctggaagat tgcgacgacg tgcaggaagt ttaccataac
     3601 ggtgaaatct ctgatgaggt tgcagcgact ctctgatgat gcctgctaaa cagcaaaacg
     3661 gagacgcgtg atggctatta ttctcggcat tgacccgggt tcgcgcgtga ccggctacgg
     3721 cgtcatccgc caggtaggta ggcaactgtc ctacctgggt agcggatgca tacgcaccaa
     3781 agtggatgat ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg aaatcatcac
     3841 ccagttccag cctgattatt tcgccattga acaagtcttt atggcgaaga acgctgactc
     3901 agccctgaaa ctgggccagg cgcgcggcgt ggcgattgtt gcggcggtga atcaggaatt
     3961 gccggtattt gaatacgcgg cacgtcaggt aaagcaaacg gtggtaggta tgggtagtgc
     4021 cgaaaaaagc caggtgcagc atatggtccg caccttgctg aaactgcccg ctaatccaca
     4081 ggcggatgcc gccgatgcgc tggcgattgc catcacccac tgccacgtta gtcagaatgc
     4141 gatgcagatg agcgaatcgc ggctgaacct gacgagaggg cgactgcgtt aagttatacc
     4201 gcctcggtca gttccggctg aggcgtttca actccctccg cgtgaatcaa caccaaaccc
     4261 ggatgatgcc acaaactggc tggcgtgacg gttttacgct cccacggaat agaacctaag
     4321 aaccagaatt tccagaaagt gagttttgca ttcggattgc tatttaacag ttgtccaaac
     4381 gtctctattt cacccaccgg aatacatagc gcctctttat aaatatcaaa gacaaatttt
     4441 gaacagaact ggcgcgaaga ttcgtattta aaacccgtgt ggtaaagttt gcgtagccgg
     4501 gagggaacct gttcaacaat tcgttgtttt tgttgttctg tcagcccagc gtctaatcgc
     4561 tttatagcat agcgttgatt agacgaacgt ttaataaaac gagatagcgt ggtgatggtt
     4621 gaaaggggaa cccggctttc tgcaaccagg aagtcttcac cgttatgacc gataatgatc
     4681 ccgacgtgat tactccagca atttgatgca gctgatattt gaccaaataa ggcagcacct
     4741 atacatgtaa agacgatatc tccaatttca tattcagcag ggtaattaat attcacgttg
     4801 tcattccatt gaaatagata caaatatacg taatagcata aaaaaattat ttttcaggag
     4861 tcgcaatgta tatttttatt acgcatttct tcactgaata tgtaatattc aaatatttgc
     4921 ttccaatata acctgttgaa taaattatac tgtgccattt ttcagatcat cgggacacct
     4981 cgcaagtttt cttcatcctt cgctggatat ctatccagca tttttttatc atacagcatt
     5041 atctttgatt cattacgcag gagcgtcatg tgataggcag actcagaggc atcatcattg
     5101 aaaaacaacc cccgctggtg ttaattgaag tgggcggcgt aggctatgaa gtgcatatgc
     5161 cgatgacctg tttttatgaa ctccctgaag cgggtcagga agcgatcgtt ttcacccact
     5221 ttgtggtgcg tgaagacgcg caactgctgt acggttttaa caataaacaa gaacgcacgt
     5281 tgttcaaaga gttgatcaaa accaacggcg tcggcccgaa gttggccctg gcgatcctct
     5341 ccggaatgtc agcgcagcag ttcgttaatg ccgttgagcg tgaagaagtg ggggcactgg
     5401 tgaaactgcc gggtattggc aaaaaaaccg ccgaacgctt gattgttgaa atgaaagacc
     5461 gatttaaagg tttgcatggc gatctcttta cgccagccgc cgacctggta ctcacgtctc
     5521 ctgctagccc ggcgaccgac gatgctgaac aagaagcagt tgccgcgctg gtggcgctag
     5581 gctataaacc acaagaagca agccgcatgg tgagcaaaat cgctcgccct gacaccagca
     5641 gtgaaacatt aattcgcgaa gccctacgcg ccgcgttatg aggtaaagga tgattgaagc
     5701 agaccgtctg atttctgccg gtaccacttt gccggaagat gtagcagatc gcgccattcg
     5761 ccctaaatta ctggaagagt atgttggtca gccgcaggtt cgttcacaga tggagatttt
     5821 catcaaagca gcgaaactgc gcggcgatgc cctcgatcat ttgttgattt ttggtcctcc
     5881 ggggttgggt aaaactacgc ttgccaacat tgtcgccaat gaaatgggcg ttaatttacg
     5941 cacgacttct ggtccggtgc tggaaaaggc gggcgatttg gctgcgatgc tcactaacct
     6001 tgaaccgcat gacgtgctgt ttattgatga gatccaccgt ttatcgccag ttgttgaaga
     6061 agtgctgtac ccggcaatgg aagactacca actggatatc atgattggtg aaggtccggc
     6121 ggcacgctcc attaaaattg atttgccgcc gtttaccctg attggcgcaa ccacgcgcgc
     6181 aggttcgctg acatcaccgt tgcgcgaccg ttttggtatt gtgcaacgtc tggagtttta
     6241 tcaggtgccg gatctgcaat atatcgtcag tcgcagtgca cgctttatgg ggcttgagat
     6301 gagtgatgac ggtgcgcagg aagtagctcg tcgcgctcgc ggtacgccac gtattgccaa
     6361 ccgtctgctg cgtcgagtgc gtgatttcgc cgaagtgaag cacgatggca ccatctcggc
     6421 agatatcgct gctcaggcgc tggatatgtt gaatgtcgat gctgaaggtt tcgattatat
     6481 ggaccgcaaa ttgttgctgg cggtaatcga taagttcttt ggtgggccgg taggtctgga
     6541 taacctggcg gcagccattg gcgaagaacg tgaaaccatt gaagatgtac tggaacctta
     6601 tttgattcag caaggctttt tgcagcgtac accgcgcggg cgtatggcga cgacgcgggc
     6661 gtggaatcac tttggcataa cgccgccaga aatgccgtaa gtcggattgg tcgctcgcgc
     6721 cgcatccgac actggcagtg tgtctgatgc gacactgttc gcgtcttatc aggccagcca
     6781 gagatagcga accgtaggtc ggataaggcg ctcgcgccgc atccgacaaa tgtgttcagc
     6841 gatagattag ctggcctgct ttttcatcat actgataata aataacagtg ccgcacatag
     6901 caccaccgac ggacccgccg gcgtatcgta aaacgcggaa aaggttaaac cgccagttac
     6961 tgccaccatc cccaccaaaa cagcgacacc agccatctgt tccggcgtac gggcaaagcg
     7021 acgcgcagta gctgcaggaa taatcagcaa cgaagtaata atcaacgcac cgacaaattt
     7081 catcgctaca ccaatcgtca atgccgtcac cagcatcaac aacaatttca cttgctgtaa
     7141 tttcacacca tcaacaaacg ccagatccgg gctaatcgtc atcgacagca aattgcgcca
     7201 ttgccagaac aaaatagcca ccacgatgac cacgccaatc gcaatagaga tgagatcttc
     7261 tggcgtcact gccagtaaat caccgaacag gtaagccatc aaatcaacac gaatgttaga
     7321 catcagacta acgaccacta ggcccagcga cagggcactg tgcgccataa tccctaataa
     7381 cgtgtcgatc gccagctgtg gacgcttctc cagccatacc agaccgcccg ccagcagcag
     7441 cgtaacggca atcaccgcat agaatggatt cacgtccagc aacaaaccaa acgcgacacc
     7501 aagtaatgag gcatgagcca gcgtatcacc gaaataagac atacgacgcc agactacaaa
     7561 cgaacccagc ggacccgcgg cacaggcgag catgatcccg gctaaccaac cgggaaataa
     7621 taattcaatc atgagcgatc atttccccga cgcaaaacaa ttcgtccctg taaatcgtga
     7681 cgatgattat gatgatggcg atagataccc agttgttcag caccacgagg accaaacatt
     7741 gaaataaact ccggatgcag ggaaacaact tccggtgtgc cggaacaaca aatgtggtga
     7801 ttcaggcaca gcacttcatc ggtttttgcc atcaccagat gcagatcgtg agaaaccatt
     7861 aaaacgccac aatccagttc acgacgcaac tggtcaataa ggtcatataa cgccacttgg
     7921 ccatttacat ccacgccttg agtgggttca tccagcacta ataattgcgg gcaatttaac
     7981 aatgctcgcg ctaatagtac acgctgcgtt tcgccaccag agagcttttg catcggtgcg
     8041 ttaatcaggt gcccggcctg gacacgtttc agtgcaggca aaatatcttc tttatgtgta
     8101 ccagggcgta agcgtaaaaa acggtttacg gtcagtggca acgtggtgtc gagatacagc
     8161 ttctgcggta catagccgat gcgcagtttt ccgttgcgct tgataacccc ttcatcgggt
     8221 gttaccagcc cgagcactac ccgtaccagt gtcgacttac ctgcgccatt tggcccaagt
     8281 aaagtcaaaa tttttccagg tttaagttcc agcgacacat cagagaggac gcggcgttgg
     8341 ccaaaagaaa ccgagacatt ttccagggaa accagacttg tcatgttaat tttagtcttg
     8401 cagtagtcat gaaatgttat aatatcacac ttctcatatt cattacgatg attggtcgca
     8461 ttatgttaca taaaaaaacg cttcttttcg cagcattatc cgccgctctc tggggaggtg
     8521 caacacaggc cgcagatgcc gccgttgtcg cttcgcttaa acccgttggg ttcatcgctt
     8581 ctgccattgc tgatggtgta acagaaacgg aggttttact gcctgacggc gcttcagaac
     8641 atgattattc actgcgtcca tcggatgtaa aacgcttaca gaacgcggac ttagtcgttt
     8701 gggttggccc ggagatggaa gcgtttatgc aaaaaccggt aagcaaatta ccagaagcga
     8761 agcaggtaac gattgcgcag cttgaagatg tgaaaccgct actgatgaaa agtattcacg
     8821 gcgatgatga tgatcacgac cacgcggaaa aaagtgacga agatcaccat cacggcgatt
     8881 tcaacatgca tctttggctt tccccagaga tagcgcgggc tacagcggtt gcaatccatg
     8941 gaaaattagt ggaacttatg ccgcaaagtc gagccaaact tgacgccaac ctgaaggatt
     9001 ttgaggcaca attggcctca accgaaacgc aggttggtaa cgagctcgcg ccgctcaagg
     9061 ggaaaggtta tttcgttttt cacgatgctt acggctattt cgaaaaacag ttcggactga
     9121 caccgcttgg tcattttacc gttaaccctg agattcaacc tggcgcgcag cgtttacatg
     9181 aaataagaac acagttggtt gagcaaaaag caacctgcgt ttttgctgag ccacagttca
     9241 ggccagcggt cgttgagagc gtcgcacgag ggacatccgt tcgtatggga acgttggatc
     9301 ccctggggac gaatatcaaa ctgggtaaaa caagctattc agaattcctg agtcaattag
     9361 ccaaccagta tgcgagctgc ctgaaaggag attaatgagg aagtgattac gtgcaacaga
     9421 tagcccgctc tgtcgccctg gcgtttaata atttaccgcg accacaccgc gttatgttgg
     9481 ggtcgctcac cgttcttact ctggccgtcg ctgtctggcg gccctatgtt tatcaccgtg
     9541 atgccacgcc aattgtcaaa accattgagc tggaacagaa cgaaattcgt tcgctcttac
     9601 ctgaagccag tgagccgatt gatcaagctg cacaagaaga tgaagccatt ccccaggacg
     9661 aactggatga caaaatcgcc ggtgaagcgg gcgtgcatga atatgttgtt tccactggcg
     9721 atacgctaag cagcattctc aatcagtatg gtattgatat gggtgatatc acccaacttg
     9781 ctgctgccga caaagaattg cgtaacctga aaatcggtca acaactctcc tggacattaa
     9841 ccgcggacgg cgaactgcag cgcctcacct gggaagtgtc tcgtcgtgaa acccgaacct
     9901 atgaccgtac tgccgctaac ggttttaaaa tgaccagcga aatgcagcaa ggagagtggg
     9961 ttaacaatct gctgaaaggt accgtcggag ggagctttgt tgccagcgcc agaaacgccg
    10021 gtttaaccag cgccgaagtg agcgcagtga ttaaagccat gcagtggcaa atggatttcc
    10081 gcaaactgaa aaaaggcgat gaatttgcgg tattaatgtc tcgagaaatg cttgatggta
    10141 aacgtgagca aagccagctg ctgggcgtac gtttgcgttc agaaggtaaa gattattacg
    10201 caatccgcgc tgaggatggc aaattctacg atcgtaacgg tactggtctg gcgaaaggat
    10261 tcttgcgatt cccgacggcg aaacagttcc gtatctcgtc taactttaac ccgcgtcgaa
    10321 ctaatccggt gacaggtcgc gttgcaccac acagaggtgt tgatttcgcc atgccacaag
    10381 gtacgccagt gctttcagtg ggtgacggtg aagtggtggt tgccaaacgc agtggcgcag
    10441 caggttatta tgtggctatt cgtcatggtc gcagctacac cacgcgttat atgcacttgc
    10501 gcaagattct ggtgaaaccg ggacagaagg tgaaacgtgg cgaccgtatc gcgctttccg
    10561 gtaataccgg acgttcaact gggccgcatc tgcactatga agtatggata aaccagcagg
    10621 ccgtaaaccc gctgacggca aaactgccgc gtaccgaagg gctgaccggc tccgatcgtc
    10681 gcgaattcct ggcgcaggcc aaagagattg tgccgcagct acggtttgat taattaacat
    10741 ccattcgcag ccggtacgca gtcagtaccg gcttttttta tttggtgcgg ggcaagttgt
    10801 gccgctacac tatcaccaga ttgatttttg ccttatccga aactggaaaa gcatggaaac
    10861 gaaaaaaaat aatagcgaat acattcctga gtttgataaa tcctttcgcc acccgcgcta
    10921 ctggggagca tggctgggtg tagcagcgat ggcgggtatc gctttaacgc cgccaaagtt
    10981 ccgtgatccc attctggcac ggctgggacg ttttgccgga cgactgggaa aaagctcacg
    11041 ccgtcgtgcg ttaatcaatc tgtcgctctg ctttccagaa cgtagtgaag ctgaacgcga
    11101 agcgattgtt gatgagatgt ttgccaccgc gccgcaagcg atggcaatga tggctgagtt
    11161 ggcaatacgc gggccggaga aaattcagcc gcgcgttggc tggcaagggc tggagatcat
    11221 cgaagagatg cggcgtaata acgagaaagt tatctttctg gtgccgcacg gttgggccgt
    11281 cgatattcct gccatgctga tggcctcgca agggcagaaa atggcagcga tgttccataa
    11341 tcagggcaac ccggtttttg attatgtctg gaacacggtg cgtcgtcgct ttggcggtcg
    11401 tctgcatgcg agaaatgacg gtattaaacc attcatccag tcggtacgtc aggggtactg
    11461 gggatattat ttacccgatc aggatcatgg cccagagcac agcgaatttg tggatttctt
    11521 tgccacctat aaagcgacgt tgcccgcgat tggtcgtttg atgaaagtat gccgtgcgcg
    11581 cgttgtaccg ctgtttccga tttatgatgg caagacgcat cgtctgacga ttcaggtgcg
    11641 cccaccgatg gatgatctgt tagaggcgga tgatcatacg attgagcggc ggatgaatga
    11701 agaagtcgag atttttgttg gtccgcgacc agaacaatac acctggatac taaaattgct
    11761 gaaaactcgc aaaccgggcg aaatccagcc gtataagcgc aaagatcttt atcccatcaa
    11821 ataaaaaagc ctctcgcgag gagaggcctt cgcctgatga taagttcaag tttgcttcag
    11881 aatattcgaa atctgttgaa ctatcattga actataggcc ggatgtggcg ttttcgccgc
    11941 atccggcaac gtacttactc taccgttaaa atacgcgtgg tattagtaga acccacggta
    12001 ctcatcacgt cgccttgggt gacaatcacc aggtcaccag acatcaagta acctttatcg
    12061 cgcagcagat taaccgcttc gctggcagct gctacgccgt cattagcgct atcaaagggc
    12121 accggcgtaa cgccacgata gagagcagtc aggttcagcg tacgttcatg gcgcgacatg
    12181 gcgaaaattg gtagaccaga gctgatacgg gaggtcatca gcgcggtacg acccgattcg
    12241 gtcatggtaa tgatcgccgt aacgcccttc aggtggttag ctgcgtacat tgccgacatg
    12301 gcaatagctt cttccacatt gtcgaactga acgtccagac ggtgtttaga aacgttgatg
    12361 ctcgggattt tttccgcacc caggcaaacg cgcgccatgg ctgcaacagt ttctgacgga
    12421 tactgcccag cggcagtttc tgcagacagc atcacagcgt cagtaccatc cagaacggcg
    12481 tttgctacgt ccatgacttc tgcacgcgtc ggcatcgggt tagtaatcat tgactccatc
    12541 atctgggtcg ccgtgattac cgctcggttt agctgacgcg cacgacggat caacgctttc
    12601 tgaatgccga ccagttccgg gtcgccaatt tccacaccga ggtcgccacg tgcaaccatt
    12661 accacgtcag aggcgaggat gatgtcatcc attgcatcct ggctgcaaac ggcttccgca
    12721 cgttcaacct tggcaacaat tttcgcatca catcctgcat cgcgtgccag gcgacgggca
    12781 tagttcagat cttcgccaca gcgtgggaag gagacagcca ggtaatctac gccaatcaac
    12841 gccgcagtct taatgtctgc tttgtctttt tcggtcagcg cttcagccga caaaccgccg
    12901 ccaagtttgt tgataccttt attgttggag aggggaccac cgacggtgac ttcggtgaac
    12961 actttcatgc cctgaacttc cagtactttt aactggacgc gaccatcgtc cagcagcagg
    13021 atgtcaccag gcacgacgtc agtaggcagg cctttgtagt cgataccgac tttttctttg
    13081 tcgccttcac ctttacccag gttggcgtcg agcaggaatt tatccccaat attgaggaaa
    13141 actttgcctt ctttaaaggt ggatacacgg attttgggcc cctggaggtc acccagaata
    13201 gccacatgac gccccagttt tgcggcaatc tcacgaactt tatccgcgcg cattttgtga
    13261 tcttcaggcg agccgtgaga aaagttcata cgtacaacgt tggcacccgc cgcgataact
    13321 ttttcaagat tattatcgcg atctgttgct gggcctaacg tggtaacgat ttttgttctg
    13381 cgaagccttt tggacatgta atactccgtt gactgaaaca accaggtgtt gcttgaacat
    13441 gaaatccgaa tgaaataacg ccgcgatggc gataaattaa tctcattcgg atgtcgtata
    13501 agattaggac agtgacagtc gcttttagcg atcgtcactt aaattaagta actgcttatc
    13561 aaaacgcgat tccttcagcg cttccttgac ccgcttcaag ttatctctga attttgcacc
    13621 gcgtcgcaaa gtaaatcctg tcgccagcac atctatcacg gtcagctgtg caagtcgaga
    13681 aaccatgggc atataaatgt cagtatcttc cggtacgtcg agggtaattg ccagcgttgc
    13741 ttcccgggcg agcggggtac ccgcagaggt gagggcaatc accatggcgt cgttttcgcg
    13801 tgccagctgc gccagctcga ccagattttt tgttcttcct gtgtgagaaa tcagcaccac
    13861 cacgtctccg tcgctacaat tcatacaact catgcgttgc agcacgatat catcggagta
    13921 caccaccgga acattaaaac gaaagaactt attcatcgca tcgtgggcaa cggcggcaga
    13981 agagcctaat ccgaaaaagg cgattttttt tgcctgagtg agcaagtcga cggcgcggtt
    14041 gatggcagat ttatccagtg aatgacggac atgatcgagc gttgccattg cggactcaaa
    14101 tattttcccc gtgtatgatt caacgctgtc atcttcattg acgttgcgat taacataggg
    14161 agtgccattc gccagacttt gtgccagatg aagtttaaaa tcaggaaaac cgcgcgtgtc
    14221 catgctgcga cagaaacgat tcaccgtcgg ttcgctaaca ttggcttcca gcgccagagc
    14281 agcaatactc gaatggatcg cgttatcggg cgaagccaga atgacctcgg caactttgcg
    14341 ctctgatttg ctcaaatgtt ccagctgaga ctggattttt tccagcatat tcatgatgta
    14401 aagagactca cgggtaatga cgatttccgc actgaaagaa atcgaaatgc agttttgtca
    14461 gatattacgc ctgtgtgccg tgttaatgac aaaagcagat aaaaaagttg ttattttttt
    14521 cataacatga tcagtgtcag atttttaccg aatggaaaac gatgattttt ttatcagttt
    14581 tgccgcgctt tgcgcgcttt tcccgtaatc gcacgggtgg ataagcgttt acagttttcg
    14641 caagctcgta aaagcagtac agtgcaccgt aagaaaatta caagtatacc ctggcttaag
    14701 taccgggtta gttaacttaa ggagaatgac atggcggtaa cgcaaacagc ccaggcctgt
    14761 gacctggtca ttttcggcgc gaaaggcgac cttgcgcgtc gtaaattgct gccttccctg
    14821 tatcaactgg aaaaagtcgg tcagctcaac ccggacaccc ggattatcgg cgtagggcgt
    14881 gctgactggg ataaagcggc atataccaaa gttgtccgcg aggcgctcga aactttcatg
    14941 aaagaaacca ttgatgaagg tttatgggac accctgagtg cacgtctgga tttttgtaat
    15001 ctcgatgtca atgacactgc tgcattcaac cgtctcggcg cgatgctgga tcaaaaaaat
    15061 cgtatcacta ttaactactt tgccatgccg cccagcactt ttggcgcaat ttgcaaaggg
    15121 cttggcgagg caaaactgaa tgctaaaccg gcacgcgtag tcatggagaa accgctgggg
    15181 acgtcgctgg cgacctcgca ggaaatcaat gatcaggttg gcgaatactt cgaggagtgc
    15241 caggtttacc gtatcgacca ctatcttggt aaagaaacgg tgctgaacct gttggcgctg
    15301 cgttttgcta actccctgtt tgtgaataac tgggacaatc gcaccattga tcatgttgag
    15361 attaccgtgg cagaagaagt ggggatcgaa gggcgctggg gctattttga taaagccggt
    15421 cagatgcgtg acatgattca gaaccacctg ctacaaattc tctgcatgat tgcgatgtct
    15481 ccgccatctg acctgagcgc agacagcatc cgcgatgaaa aagtgaaagt actgaagtcc
    15541 ctgcgccgca tcgaccgctc caacgtacgc gaaaaaaccg tacgcgggca atatactgcg
    15601 ggcttcgccc agggcaaaaa agtgccggga tatctggaag aagagggcgc gaacaaaagc
    15661 agcaatacag aaaccttcgt ggcgatccgc gtcgacattg ataactggcg ctgggccggt
    15721 gtgccattct acctgcgtac tggtaaacgt ctgccgacca aatgttctga agtcgtggtc
    15781 tatttcaaaa cacctgaact gaatctgttt aaagagtcgt ggcaggatct gccgcagaat
    15841 aaactgacta tccgtctgca acctgatgaa ggcgtggata tccaggtact gaataaagtt
    15901 cctggccttg accacaaaca taacctgcaa atcaccaagc tggatctgag ctattcagaa
    15961 acctttaatc agacgcatct ggcggatgcc tatgaacgtt tgctgctgga aaccatgcgt
    16021 ggtattcagg cactgtttgt acgtcgcgac gaagtggaag aagcctggaa atgggtagac
    16081 tccattactg aggcgtgggc gatggacaat gatgcgccga aaccgtatca ggccggaacc
    16141 tggggacccg ttgcctcggt ggcgatgatt acccgtgatg gtcgttcctg gaatgagttt
    16201 gagtaatatc agcgcttatc ctttatgggt attttaccgg taacatgatc ttgcacagat
    16261 tgtagaacaa tttttacact ttcaggcctc gtgcggattc acccacgagg ctttttttat
    16321 tacactgact gaaacgtttt tgccctatga gctccggtta caggcgtttc actcataaat
    16381 cctctgaatg aaacgcgttg tgaatcatcc tgctctgaca actcaatttc aggagccttt
    16441 atgaatccac aattgttacg cgtaacaaat cgaatcattg aacgttcgcg cgagactcgc
    16501 tctgcttatc tcgcccggat agaacaagcg aaaacgtcga ccgttcatcg ttcgcagttg
    16561 gcatgcggta acctggcaca cggtttcgct gcctgccagc cagaaaacaa agcctctttg
    16621 aaaagcatgt tgcgtaacaa tatcgccatc atcacctcct ataacgacat gctctccgcg
    16681 caccagcctt atgaacacta tccagaaatc attcgtaaag ccctgcatga agcgaatgcg
    16741 gttggtcagg ttgcgggcgg tgttccggcg atgtgtgatg gtgtcaccca ggggcaggat
    16801 ggaatggaat tgtcgctgct aagccgcgaa gtgatagcga tgtctgcggc ggtggggctg
    16861 tcccataaca tgtttgatgg tgctctgttc ctcggtgtgt gcgacaagat tgtcccgggt
    16921 ctgacgatgg cagccctgtc gtttggtcat ttgcctgcgg tgtttgtgcc gtctggaccg
    16981 atggcaagcg gtttgccaaa taaagaaaaa gtgcgtattc gccagcttta tgccgaaggt
    17041 aaagtggacc gcatggcctt actggagtca gaagccgcgt cttaccatgc gccgggaaca
    17101 tgtactttct acggtactgc caacaccaac cagatggtgg tggaatttat ggggatgcag
    17161 ttgccaggct cttcttttgt tcatccggat tctccgctgc gcgatgcttt gaccgctgcc
    17221 gctgcgcgtc aggttacacg catgaccggt aatggtaatg aatggatgcc gatcggtaag
    17281 atgatcgatg agaaagtggt ggtgaacggt atcgttgcac tgctggcgac cggtggttcc
    17341 actaaccaca ccatgcacct ggtggcgatg gcgcgcgcgg ccggtattca gattaactgg
    17401 gatgacttct ctgacctttc tgatgttgta ccgctgatgg cacgtctcta cccgaacggt
    17461 ccggccgata ttaaccactt ccaggcggca ggtggcgtac cggttctggt gcgtgaactg
    17521 ctcaaagcag gcctgctgca tgaagatgtc aatacggtgg caggctttgg tctgtctcgt
    17581 tatacccttg aaccatggct gaataatggt gaactggact ggcgggaagg ggcggaaaaa
    17641 tcactcgaca gcaatgtgat cgcttccttc gaacaacctt tctctcatca tggtgggaca
    17701 aaagtgttaa gcggtaacct gggccgtgcg gttatgaaaa cctctgccgt gccggttgag
    17761 aaccaggtga ttgaagcgcc agcggttgtt tttgaaagcc agcatgacgt tatgccggcc
    17821 tttgaagcgg gtttgctgga ccgcgattgt gtcgttgttg tccgtcatca ggggccaaaa
    17881 gcgaacggaa tgccagaatt acataaactc atgccgccac ttggtgtatt attggaccgg
    17941 tgtttcaaaa ttgcgttagt taccgatgga cgactctccg gcgcttcagg taaagtgccg
    18001 tcagctatcc acgtaacacc agaagcctac gatggcgggc tgctggcaaa agtgcgcgac
    18061 ggggacatca ttcgtgtgaa tggacagaca ggcgaactga cactgctggt agacgaagcg
    18121 gaactggctg ctcgcgaacc gcacattcct gacctgagcg cgtcacgcgt gggaacagga
    18181 cgtgaattat tcagcgcctt gcgtgaaaaa ctgtccggtg ccgaacaggg cgcaacctgt
    18241 atcacttttt aagacgacaa atttgtaatc aggcgagaga aaactctgat gaaaaactgg
    18301 aaaacaagtg cagaatcaat cctgaccacc ggcccggttg taccggttat cgtggtaaaa
    18361 aaactggaac acgcggtgcc gatggcaaaa gcgttggttg ctggtggggt gcgcgttctg
    18421 gaagtgactc tgcgtaccga gtgtgcagtt gacgctatcc gtgctatcgc caaagaagtg
    18481 cctgaagcga ttgtgggtgc cggtacggtg ctgaatccac agcagctggc agaagtcact
    18541 gaagcgggtg cacagttcgc aattagcccg ggtctgaccg agccgctgct gaaagctgct
    18601 accgaaggga ctattcctct gattccgggg atcagcactg tttccgaact gatgctgggt
    18661 atggactacg gtttgaaaga gttcaaattc ttcccggctg aagctaacgg cggcgtgaaa
    18721 gccctgcagg cgatcgcggg tccgttctcc caggtccgtt tctgcccgac gggtggtatt
    18781 tctccggcta actaccgtga ctacctggca ctgaaaagcg tgctgtgcat cggtggttcc
    18841 tggctggttc cggcagatgc gctggaagcg ggcgattacg accgcattac taagctggcg
    18901 cgtgaagctg tagaaggcgc taagctgtaa aagtcaaaat gcccgatcta ggatcgggca
    18961 tttttgtagc gtttttttgc cgggtttaac cctgtacttt tacctgtccg gcggcgtgct
    19021 tcgcgcgttc aatggcgtca acaacactct ctgaagtagc cagtaccacc cccagatgac
    19081 ggctgccatc aatttccggc ttaccaaata aacgaatctg caaatctgcg cctacggcat
    19141 tctgcacatt atcaaacgtg acattctgac tggtcagttg tggcagaata acggcagaag
    19201 ctgcaggacc atactgacgg atcccgccaa caggaagtcc gaggaaggca cgtacatgca
    19261 gggcaaactc tgagagatct tgagaaatta acgtcaccat cccggtatca tgtggacgag
    19321 gggagacctc actgaaaatc acctcatcac cacagacaaa cagctcgaca ccaaacagac
    19381 cataaccgcc cagtgccagc accactttac gggcaatctc ctgcgcacgt tcaagggcaa
    19441 gcgggctcat ttgctgtggt tgccaggatt cacggtagtc gccatcttcc tggcgatgac
    19501 ctactggtgc acagaaatgg acgccatcca ccgcgctgac ggttagcagg gtaatttcga
    19561 agtcaaactt aacgacgcct tcaacaatta cgcggcccgc tccggcgcga ccgccttgct
    19621 gagcgtattc ccatgcctga gcaagttgct ctgcagagcg aataaacgtc tgccccttgc
    19681 cggaagagct catcaccggt tttacaatgc agggatagcc aatggcggca accgcctcgc
    19741 ggaaaaggct tttgctatcg gcaaaacgat aagtggaagt gggcagctgc agctcttctg
    19801 ccgccaggcg acggataccc tcgcgattca tcgttaattt cgttgcgcga gcgcagggga
    19861 caacattcag tccttcctct tcaagttgga tcagcatatc ggtggcaata gcttcgatct
    19921 ccggcacgat ataatgtggt ttttccagtt caaccacacg gcgtaatgca tcaccatcaa
    19981 gcatattaat gacatgggag cgatgcgcga catgcatggc tggtgcgtcg gcatagcgat
    20041 cgacggcaat cacctctacg ccgagacgct gacactcgat tgccacttct ttacccagtt
    20101 caccggagcc taataacatc acgcgagttg ctgccggacg cagcgcagtg cctaataacg
    20161 tcataacgaa tatcccctga ttaattccgc gcgcagtata aacgaaaacg tttgcgtgtg
    20221 tctttattgc tgatgttgat ttctcaaccg aaaagaaata tactgtataa aatctcagtt
    20281 attatgagag gctgcaaatg gcggttgaag ttaaatacgt agtcattcgt gagggcgaag
    20341 agaaaatgtc gtttaccagc aaaaaggaag ccgatgctta tgacaaaatg cttgatacgg
    20401 cggatctgct tgacacctag ctgacaaatt ctccagtgca aatggaagac gagcaacgtg
    20461 aagccctttc gctatggctg gcagaacaaa aagaggtgct gagcaccatt ctgaaaaccg
    20521 gcaaattacc gtccccacag gtcgtcggtg ctgaatccga agaggaagat gcctcacacg
    20581 cggcctgaag caacttgcgc tcctgacaat ttgtaccatg ctttctcctg aatcattgtg
    20641 ctatggagaa aaacatgaaa aaaagagggg cgtttttagg gcggttgttg gtttctgcct
    20701 gcgcatcagt tttcgctgcc aataatgaaa ccagcaagtc ggtcactttc ccaaagtgtg
    20761 aaggtctgga tgctgccgga attgccgcga gcgtaaaacg tgattatcaa caaaatcgcg
    20821 tggcgcgctg ggcagatgat caaaaaattg tcggtcaggc cgatcccgtg gcctgggtca
    20881 gtttgcagga cattcagggt aaagatgata aatggtcagt accgctaacc gtgcgtggta
    20941 aaagtgccga tattcattac caggtctgcg tggactgcaa agcgggaatg gcggaatatc
    21001 agcggcgtta atggcttctg ataattcgat tgaccgttgg cggatgcggc gcgagtgcct
    21061 tgtccgtccc agaacgcacc acaatcgtag gcctgagaag acgcaacagc gtcgcatcgg
    21121 gcaatcagca tcaaacaatg ccactcaaaa cgacaaacta ctttcaccta aaataccctt
    21181 atcacctgat atacagtaaa taccttacct ggcgcgaatt tctttgaata acggcctacc
    21241 gccccgctta tttgcgcatc ttttgtctcg atatacattt tctgacatcc tcatccgcta
    21301 tgctacaggc actaaaaatg gttattgagg agcaaatatg gctaactggt taaatcaact
    21361 gcaatccctg cttgggcaaa gcagttcttc tacctcctcg tctgcggatc agggattggg
    21421 caaactgtta gtgtcaggcg cattaggcgg actggctggg ctgctggtcg caaataaatc
    21481 agcacgtaaa cttcttacga aatatggcac caacgcgtta ctggttggcg gcggagcggt
    21541 agcgggtacg gtgctgtgga ataaatacaa agataaaatt cgcgcggcgc atcaggacga
    21601 accgcagttt ggcgcgcaaa gtacgccgct ggatgagcgt acggaacgtt tgatccttgc
    21661 gctggtcttt gccgctaaaa gtgatggtca tattgatgcc aatgaacgtg cggcaatcga
    21721 ccagcaattg cgtgaagccg gcgtggaaga gcaggggcgt gtactcattg agcaggcaat
    21781 cgaacaaccg ctggatccac aacgcctggc taccggggtc cgcaatgaag aagaggcgct
    21841 ggaaatctat ttcctgagct gcgcggctat tgatattgac cattttatgg aacgcagtta
    21901 cctgaatgca ctgggtgacg cgctgaaaat cccccaggat gttcgtgaag gcattgaacg
    21961 cgatctcgaa cagcaaaaac gcactttggc ggaataattc cgctctctgg atagcctgaa
    22021 gttgactatt tcgggctatc cagaaccgca cagccatttg aacaggctga ttttctctgt
    22081 ctgcataaca ttcgatatcc gcgccattac ctgtcatcat ctaagcaatg acgaccctgt
    22141 ttcgcttgca tccccggtga gttttgccac ccttataaga tgtttccaac cagaaagaac
    22201 aataacatgc taccaaaagc cgcccgcatt ccccacgcca tgacgcttca tggcgatacg
    22261 cgcatcgata attactactg gctgcgggac gatacgcgtt ctcagccaaa agtcctggac
    22321 tacctgcaac aagaaaatag ttacggtcat cgggtgatgg cctcacaaca agccttgcag
    22381 gatcgcatct taaaggaaat catcgaccgc attccgcaaa gagaagtttc tgcgccctac
    22441 ataaaaaatg gctaccgcta tcggcatatt tatgaaccag gctgtgaata tgctatctac
    22501 cagcgtcaat cggcattcag cgaagagtgg gatgagtggg aaacattgct cgatgccaac
    22561 aagcgcgcag ctcatagtga gttttattcg atgggcggaa tggcgattac gcccgataac
    22621 accattatgg cgctggcaga agattttctt tcccgacgcc agtacggcat tcgttttcgt
    22681 aatctggaaa caggtaactg gtacccggaa ctgctggata acgttgaacc cagctttgtc
    22741 tgggcaaatg actcctggac tttctactat gttcgcaagc atccggtgac gctgctgcct
    22801 tatcaggtct ggcgtcacgc catcggtacg ccagcatcgc aagatacact gatctacgaa
    22861 gaaaaagacg atacctatta tgtcagcctg cataaaacga cgtcgaagca ctatgtcgtc
    22921 attcatctgg ccagcgccac caccagtgaa gttcgcctgc tggacgcgga aatggccgat
    22981 gccgagccgt ttgtttttct gccgcgccgc aaagatcacg aatacagcct tgatcactac
    23041 cagcatcgtt tttatctgcg ttccaatcgc cacggcaaaa actttggctt ataccgtacc
    23101 cgtatgcgtg atgagcaaca gtgggaagag ttaattccgc cacgcgaaaa catcatgctg
    23161 gaagggttta cgctgtttac cgactggctg gtggttgaag agcgtcagcg cgggttaacc
    23221 agtttgcgcc aaattaaccg caagacccgg gaagtcattg gcattgcctt tgatgatccg
    23281 gcctatgtga cctggattgc ctacaatcca gaacctgaaa ccgcgcgatt gcgttatggt
    23341 tattcttcca tgaccacacc agacactttg tttgaactgg atatggatac cggtgagcgt
    23401 cgtgtattaa aacaaacaga agtgcctggt tttgatgcgg cgaattaccg cagtgaacac
    23461 ctgtggatag tcgcccgtga tggcgtcgaa gttccggttt cgctggtcta tcatcgcaaa
    23521 cattttcgca aaggacacaa cccgttgctg gtgtatggct atggttctta cggcgcaagt
    23581 attgatgccg atttcagttt tagccgcttg agtttgttag atcgtggctt tgtctacgcc
    23641 attgtccatg ttcgcggcgg tggtgagctg gggcaacaat ggtacgaaga cggtaaattt
    23701 ctgaagaaga aaaatacgtt taatgattat cttgatgcct gcgatgcatt gttaaaactg
    23761 ggctatggct ctccttcgct ctgttatgcg atgggcggaa gtgcgggggg catgttgatg
    23821 ggcgttgcaa ttaatcaacg cccggaatta ttccacggcg ttatcgccca ggtaccgttt
    23881 gttgatgttg taacaacgat gcttgatgaa tcaattcctc ttaccactgg tgagtttgaa
    23941 gagtggggaa atccgcagga tccgcaatat tacgagtaca tgaaaagcta cagcccgtat
    24001 gacaacgtca ccgcacaggc ttatccgcat ttactggtaa cgaccggttt acacgattct
    24061 caggtgcaat attgggaacc ggcaaaatgg gtcgctaaat tgcgcgagct gaaaaccgat
    24121 gaccatcttt tattgctctg taccgacatg gactcaggcc atggcggtaa atctggccgc
    24181 tttaaatcgt acgaaggcgt agcgatggaa tatgcttttc tggtcgcgct ggcgcaggga
    24241 acattacccg ctacgcctgc ggattaagta ttttccagat aatgtttcag tgttaaacgc
    24301 agctccgggc tcatgctgtc caggttatta aataaccagc gcagatagcc cggatagcgt
    24361 tcggcaacgt cggaaaccgc tttgccacgg tatttgccaa aggtgaaggt tgtcatcaac
    24421 gacggacgtc cggtgatatc ggccatctgt tctgccgtcc agccggaggt gttcataata
    24481 tcgataagca acgcggcggt gatataacag tcatacaacg cgcggtgatg atgcaggccc
    24541 agcggtgtct gtacattgag cttgcgtgtt ttatataacg ccatattgct gtacttgagc
    24601 cctggccaca aacgacgggc cagtttcata gtgcaaatcc actcaccggg catctcaggc
    24661 agtacgcggc ggtcaaagct ggcgttgtgc gcgacatacc attcactacc gtaatagtgt
    24721 gggatcacat cttcaatcca cggtttatcg gcgaccatgg cttcggtgat gcgatgaatc
    24781 gccatcgctt gtggactaat aggacgatcg gggcgcacca ggaggctcat ggggttgacg
    24841 atttttccgt caatgacatc aacagaggca atctcaacga tccctccctg caaaccgcag
    24901 gtttctgtat cgataatgcg caacatggca tgatccaggc cgaaaaagcc tagcgtaatg
    24961 gaatgatatc gccttgccaa ccctgtgatg aacgctgacc gactaacaat aatgaaccac
    25021 gatcggcgcg aacgatgaga cgaccatatt catcccatag tgcactgtta ccacgggcat
    25081 ttgccatcag cacggcaatc gagtactgat gtgaaaaaaa ttgtaagcga cgggccgacg
    25141 ccagcaggtc gggttctccc agacattgcc ctgtcgtaaa cagtgaacag gtcgggtcca
    25201 tatccatacc ttgcggttgt tcatcgacca ccgtgatggt tctggaacga cggcccagac
    25261 atgcaccatg actctgatgg tagttcccag gcgtttttcg ccacggtgcg aacaccgcaa
    25321 ttccacgaat aaagcgatcg ttatattcaa cgggaaggcc ggcaatgatg gtcattcgct
    25381 aggtcgttgc ggcataacaa agcgggtcta acagtgaaag atcgggtggg gcaggaaggg
    25441 cacgtcgcga ataatcacac cccagtaaag aaagcgatgg aaaaactaac agctgacatt
    25501 gttgcctggc ggcggccctc acaaactcca gatgatgagc tacctgttcg gtgagtgagg
    25561 ttttacgagg ctcatattgc gctgctgcaa ctttccagaa cgacataatg acttcctttt
    25621 caaaatgact gcaaattatg ggggcggttt agaaaatact cataatttag caggcgttat
    25681 gtaagaaagt gtaactctgt ataagtttta tttaagtttg ggctcgtaag gtaaacgtga
    25741 cagattgacc gaagccaaac ggtgggcaat aagccgctcg cgaaaccagc tgcgcaaatg
    25801 ttctggctgt tcacgttcaa ccgcttcagc gatcaccggc atattgtagc gttctttaaa
    25861 agccaccccg gccgccgcca aatcgacatt cactttatcc atttctgttt gatccagttt
    25921 agccagattc ttcagcattc ttaatctcct catcattcgc gggcaaaagt tactaagagt
    25981 gagcctggat ggcaagccgg aattgatgat cctcgactca gacattagac aaggttatgc
    26041 tctgtaaaaa agttattaca aaaaggaata gataatatgg cttcaactgc acgctccctt
    26101 cgctacgcgc tggcaatcct gaccacttcg ttggtgacac cttccgtctg ggcacatgcg
    26161 catttaacgc atcagtatcc tgcggc
//
LOCUS       DAZOZX010000048        25659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZOZX010000048 DAZOZX010000000
VERSION     DAZOZX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.048"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUPT_001938"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUPT_001938"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR0819523.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUPT_001939"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUPT_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0819524.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHUPT_001940"
     CDS             2630..3391
                     /locus_tag="ACHUPT_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0819525.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHUPT_001941"
     CDS             complement(3442..4326)
                     /locus_tag="ACHUPT_001941"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0819526.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUPT_001942"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUPT_001942"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0819527.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUPT_001943"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUPT_001943"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0819528.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHUPT_001944"
     CDS             6202..6474
                     /locus_tag="ACHUPT_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0819529.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHUPT_001945"
     CDS             6844..7920
                     /locus_tag="ACHUPT_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0819530.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHUPT_001946"
     CDS             7947..9326
                     /locus_tag="ACHUPT_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0819531.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHUPT_001947"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHUPT_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHUPT_001948"
     CDS             10409..11245
                     /locus_tag="ACHUPT_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR0819532.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHUPT_001949"
     CDS             11250..12197
                     /locus_tag="ACHUPT_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0819533.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHUPT_001950"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHUPT_001950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR0819534.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACHUPT_001951"
     CDS             13324..14082
                     /locus_tag="ACHUPT_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0819535.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACHUPT_001952"
     CDS             14200..15558
                     /locus_tag="ACHUPT_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0819536.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHUPT_001953"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHUPT_001953"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0819537.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHUPT_001954"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHUPT_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0819538.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHUPT_001955"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHUPT_001955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0819539.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACHUPT_001956"
     CDS             19503..20054
                     /locus_tag="ACHUPT_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0819540.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACHUPT_001957"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACHUPT_001957"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0819541.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACHUPT_001958"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACHUPT_001958"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0819542.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACHUPT_001959"
     CDS             23307..24347
                     /locus_tag="ACHUPT_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0819543.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHUPT_001960"
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHUPT_001960"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0819544.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6624 g         6547 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 taatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DAZOZX010000049        24961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZOZX010000049 DAZOZX010000000
VERSION     DAZOZX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.049"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="ACHUPT_001961"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="ACHUPT_001961"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0819545.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="ACHUPT_001962"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="ACHUPT_001962"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0819546.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="ACHUPT_001963"
     tRNA            2652..2727
                     /locus_tag="ACHUPT_001963"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            complement(2742..3896)
                     /locus_tag="ACHUPT_001964"
     CDS             complement(2742..3896)
                     /locus_tag="ACHUPT_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0819547.1"
                     /translation="MKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAEAW
                     RVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEKIM
                     EAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMAVE
                     WGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNVTD
                     LEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPAYR
                     SDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALKEM
                     GGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3779..4126)
                     /locus_tag="ACHUPT_001965"
     CDS             complement(3779..4126)
                     /locus_tag="ACHUPT_001965"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0819548.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAAKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(4213..4557)
                     /locus_tag="ACHUPT_001966"
     CDS             complement(4213..4557)
                     /locus_tag="ACHUPT_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819549.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(4658..4837)
                     /locus_tag="ACHUPT_001967"
     CDS             complement(4658..4837)
                     /locus_tag="ACHUPT_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HFR0819550.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(4934..5863)
                     /locus_tag="ACHUPT_001968"
     CDS             complement(4934..5863)
                     /locus_tag="ACHUPT_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0819551.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(5866..6057)
                     /locus_tag="ACHUPT_001969"
     CDS             complement(5866..6057)
                     /locus_tag="ACHUPT_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819552.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(6059..6688)
                     /locus_tag="ACHUPT_001970"
     CDS             complement(6059..6688)
                     /locus_tag="ACHUPT_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR0819553.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(6685..7260)
                     /locus_tag="ACHUPT_001971"
     CDS             complement(6685..7260)
                     /locus_tag="ACHUPT_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HFR0819554.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(7257..7421)
                     /locus_tag="ACHUPT_001972"
     CDS             complement(7257..7421)
                     /locus_tag="ACHUPT_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFR0819555.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(7432..7725)
                     /locus_tag="ACHUPT_001973"
     CDS             complement(7432..7725)
                     /locus_tag="ACHUPT_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFR0819556.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(7749..8132)
                     /locus_tag="ACHUPT_001974"
     CDS             complement(7749..8132)
                     /locus_tag="ACHUPT_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819557.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(8132..8737)
                     /locus_tag="ACHUPT_001975"
     CDS             complement(8132..8737)
                     /locus_tag="ACHUPT_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFR0819558.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(8994..9146)
                     /gene="kil"
                     /locus_tag="ACHUPT_001976"
     CDS             complement(8994..9146)
                     /gene="kil"
                     /locus_tag="ACHUPT_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFR0819559.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(9131..9265)
                     /locus_tag="ACHUPT_001977"
     CDS             complement(9131..9265)
                     /locus_tag="ACHUPT_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819560.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQLEALCAMK
                     "
     gene            complement(9460..9930)
                     /locus_tag="ACHUPT_001978"
     CDS             complement(9460..9930)
                     /locus_tag="ACHUPT_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819561.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(9939..10265)
                     /locus_tag="ACHUPT_001979"
     CDS             complement(9939..10265)
                     /locus_tag="ACHUPT_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFR0819562.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(10746..10940)
                     /locus_tag="ACHUPT_001980"
     CDS             complement(10746..10940)
                     /locus_tag="ACHUPT_001980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819563.1"
                     /translation="MNGDRYEFTKDSTEDSVFHVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVET"
     gene            complement(11030..11662)
                     /locus_tag="ACHUPT_001981"
     CDS             complement(11030..11662)
                     /locus_tag="ACHUPT_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFR0819564.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            11766..11981
                     /locus_tag="ACHUPT_001982"
     CDS             11766..11981
                     /locus_tag="ACHUPT_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0819565.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            12098..12379
                     /locus_tag="ACHUPT_001983"
     CDS             12098..12379
                     /locus_tag="ACHUPT_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFR0819566.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            12414..12575
                     /locus_tag="ACHUPT_001984"
     CDS             12414..12575
                     /locus_tag="ACHUPT_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819567.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            12562..13392
                     /locus_tag="ACHUPT_001985"
     CDS             12562..13392
                     /locus_tag="ACHUPT_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR0819568.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            13389..14765
                     /locus_tag="ACHUPT_001986"
     CDS             13389..14765
                     /locus_tag="ACHUPT_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0819569.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            14838..15164
                     /locus_tag="ACHUPT_001987"
     CDS             14838..15164
                     /locus_tag="ACHUPT_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819570.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            15161..15361
                     /locus_tag="ACHUPT_001988"
     CDS             15161..15361
                     /locus_tag="ACHUPT_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819571.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            15373..15561
                     /locus_tag="ACHUPT_001989"
     CDS             15373..15561
                     /locus_tag="ACHUPT_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819572.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            15564..15728
                     /locus_tag="ACHUPT_001990"
     CDS             15564..15728
                     /locus_tag="ACHUPT_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819573.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            15709..16149
                     /locus_tag="ACHUPT_001991"
     CDS             15709..16149
                     /locus_tag="ACHUPT_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFR0819574.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            16146..16736
                     /locus_tag="ACHUPT_001992"
     CDS             16146..16736
                     /locus_tag="ACHUPT_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFR0819575.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            16733..16906
                     /gene="ninD"
                     /locus_tag="ACHUPT_001993"
     CDS             16733..16906
                     /gene="ninD"
                     /locus_tag="ACHUPT_001993"
                     /inference="COORDINATES: protein motif:HMM:NF036649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinD"
                     /protein_id="HFR0819576.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            16873..17048
                     /locus_tag="ACHUPT_001994"
                     /pseudo
     CDS             16873..17048
                     /locus_tag="ACHUPT_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            17051..17452
                     /locus_tag="ACHUPT_001995"
     CDS             17051..17452
                     /locus_tag="ACHUPT_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819577.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            17412..17642
                     /locus_tag="ACHUPT_001996"
     CDS             17412..17642
                     /locus_tag="ACHUPT_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFR0819578.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            17614..18225
                     /locus_tag="ACHUPT_001997"
     CDS             17614..18225
                     /locus_tag="ACHUPT_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFR0819579.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            18222..18428
                     /locus_tag="ACHUPT_001998"
     CDS             18222..18428
                     /locus_tag="ACHUPT_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFR0819580.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            18406..19071
                     /locus_tag="ACHUPT_001999"
     CDS             18406..19071
                     /locus_tag="ACHUPT_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR0819581.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            19068..19691
                     /locus_tag="ACHUPT_002000"
     CDS             19068..19691
                     /locus_tag="ACHUPT_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0819582.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            20124..20447
                     /locus_tag="ACHUPT_002001"
     CDS             20124..20447
                     /locus_tag="ACHUPT_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFR0819583.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            20431..20907
                     /locus_tag="ACHUPT_002002"
     CDS             20431..20907
                     /locus_tag="ACHUPT_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR0819584.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            20904..21341
                     /locus_tag="ACHUPT_002003"
     CDS             20904..21341
                     /locus_tag="ACHUPT_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0819585.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            21546..22088
                     /locus_tag="ACHUPT_002004"
     CDS             21546..22088
                     /locus_tag="ACHUPT_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0819586.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSDTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            22308..22523
                     /locus_tag="ACHUPT_002005"
     CDS             22308..22523
                     /locus_tag="ACHUPT_002005"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0819587.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            22671..22913
                     /locus_tag="ACHUPT_002006"
     CDS             22671..22913
                     /locus_tag="ACHUPT_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFR0819588.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            22949..23437
                     /locus_tag="ACHUPT_002007"
     CDS             22949..23437
                     /locus_tag="ACHUPT_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0819589.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            23415..24914
                     /locus_tag="ACHUPT_002008"
     CDS             23415..24914
                     /locus_tag="ACHUPT_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR0819590.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
BASE COUNT         6677 a         5878 c         6110 g         6296 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
     1741 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 cagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac
     2161 agttgcaggc aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccattt aaatcaataa gttacctcgc atttaagtaa
     2761 accacgttct cctcttgtgc cgtatttgtg ccattgcgac ttataatcgc atcgattttg
     2821 cttgcgtgct cggtgagatg cccggctgaa aggtgggcgt atctttgaac catttcgaga
     2881 gtttcccatc ctcccatctc tttaagtgca agaagagaga caccggactg aaccagccag
     2941 cttgcccagg tatgcctcag atcatggaag cggaagttgc taatgcctgc ccgctttaac
     3001 gctcccttcc atgccttgtt gctgtcggtt ctcatcttcc ttaccgctgc tgtttttgtt
     3061 ccgtcgcttc ggtaggcagg tttggtgtgg acaaataccc atctcttatg gagcccctgc
     3121 tgttttctta atatctggca tgcggtttcg ttaagaggaa ctccgatcgc attgccagct
     3181 tttgtttcat cagggtgcat ccatgccatt ttcttatcca gatcgacctg tgaccactca
     3241 aggtctgtaa cgttggaacg gcgaaggcct gtcgtgattg caaacatgac cacagggaag
     3301 aagtgaggag caatttctgc aaacaggcgc ttcgattcct cctctgtaag ccatctgatg
     3361 cgtccattct taacgcgtgg tgttgatatt ttgggtgccc tgtcaagcca tccccattca
     3421 acagccatat tgagaatggc gcgaagtatt gccagatgcc tcgtcttcgt tcctttgctt
     3481 gcaagctttg gtttatactc cggcaccgga ttgccaagtc gcaaacacct gtcccggctc
     3541 atctcccagt tcaggcgatg gcggcggttt tccatcccgt ctaccgcctc cattattttt
     3601 tctgttgtta tgtcagagag aatggcttct ctgaagtgca acatccagaa cgatataatg
     3661 ctcttgtcat catcaatgga cttcttatcc gatttctcac gcagccaccg tatgcaggct
     3721 tccttgaata gctttttcgg tgattccccg agatttttta ctctccacgc ttctgctttc
     3781 agacgatcgt gaagttcttg cgcttgcctt ttgtccgatg tttcaagaga gcgtctaact
     3841 cttgatccat ctggcgcgac gaaatcgcag tgccacgtgc caccgcgtag tttgattgac
     3901 atgctttaac ctcctgcaca tcaaccgcat tcaccgcgct attgtgtctc acagacttaa
     3961 gcgccgcaat gcagtctgac ttgcaaatgc gatatgggct tttaggttta tctggattta
     4021 tctttgcggc ctgaagtcgt ccacttcgta tccactgcgt gatagtgcct ttgtctacct
     4081 tcagatacga cgctgcctct tcacgagtga agatttcttc ttccacctgg aatctccatt
     4141 tattggattg gtattattgc ggtaggtctg gatatcattg agcaatgaac aggcctcatc
     4201 gagtgtgagg cgttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt
     4261 gttcccgtac gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag
     4321 gattcctttg aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg
     4381 aatctgctca acaagtgcaa cgcatcgccg gaaatcggct gcgtcatgtg gagcaccgaa
     4441 gctatgacca tagatattct ttttcagtcc acatgcgatt gaggcaagag ttgcgctact
     4501 gatgccgaca tcgtcagtcg attgccattt caaaaccttc atagccaaat ctgacatttc
     4561 ttgtctccaa taaaaaaccg ccatcaggcg gcttggtgtt ctttcaattc ttcaattcga
     4621 atattggtta cattgttttc atatatgaat aaataaatta gcttttttcg ttgccttcgc
     4681 gttctttatt aattttgaca aactcgtttt taccacgctc tccaaatgcg tctttagagt
     4741 cgttgtatcc gcaatcgcag cacacataat caccagacca tccacgcatt gttttttctt
     4801 ttgcaatatt tccagaaccg cattttggac aagacatatc actacctcca aagcatgagt
     4861 gagatgacaa cgtaacattg attggagatt aacaatagat tgctgatgta aaagatatgt
     4921 ataagcttcg ctttcaaagt ggaggctctg gtagcggcat ccagtgggtt acattgctaa
     4981 tcagaccata ttcattagtt tgaggatggt tgccgttatc gtctccgtat ttaagactct
     5041 ccataaaacc ataatgctta ttcccattaa cgctcacaaa gccgtaataa gctggtataa
     5101 cgccgatctc acacgtaatc agcaaaggaa aactagttct ccaatttagc tcgccgatta
     5161 caggcattct ctcactacag cttatccaac catccggagt taccggaact tgcggaatgg
     5221 ctgtctgctc tcgaacgtca ttaggcgcga taggttctgc tgccaactga ctggcatatt
     5281 tgttaatggt aacgataagc tcttgctcag cctcatccag acaatcaccg atacctcgcc
     5341 tgtcaccgtc aaaatcatcg aaatcggcac gaatcttggc aaccttctgg attgcggaca
     5401 acacctcact aggaattacc ggatagttgg ttgacgtttc cgcgatttca cgaaaattat
     5461 tggttgacga attcttgttt tcccgaaagt ttccggactg aagcatggct gcgcgatagg
     5521 cgttccagcc gacagctttt ccgtgttcaa acgcgctgtc aaagtcatca tccatttcca
     5581 tcgcatcagg cacagatacc gtctctggag gggcggcgta aagcggagtt acggtaattt
     5641 cgtactcgtt tatatcctcg tcgctgatat ttgcgaaata gcgaaggatg cctttctctc
     5701 cacgactacg agcgtcagag tcctgaatga tatacgccac aggctctgct tccagcgatg
     5761 ccagcgcaat tcgtgccagt tccatttgtt cgccacgagt aagcccgttt tcaagcggat
     5821 ttttaatgaa caattcaata cgttctttgg taatagtggt catagctatt tcaccttaat
     5881 ctcaatattt cgcagcttta gctctactgg caggtctgac tttcctgtta acgctaatgc
     5941 gagattttct ggagtaatga gagcagttat tgttttcccc atcgccagac gaataatcag
     6001 tcgtatctcg cgatcgtcac atgctcctgg tcgaacaatt gatatttgtc cgtccatctc
     6061 actctccttt gatgcgaatg ccagcggcgc ggattgcagc gatgacctca gaaactttgt
     6121 atgccattac cgtttggtaa tcatcgtgaa aatctgttcg atgaagcatg ctgctacgtt
     6181 ccgggagcag tattccccgc tcccccagtt ctgctatgcg attctctgcg gattccagcg
     6241 ccgcaaccaa ttcgtctaca gttccggcag cttgcagtgc gtaatcggta atagccatct
     6301 catgatcaat ttcagtaccg ttctcattcg ttgaggtgat agcaaaataa tcagagtcga
     6361 tttcgttatc agctaagtgg cgtagcgtat cggcaacaag ccggccgttt tcgattagca
     6421 gcttccctac cgttagcgca atatcctcgt tctcctggtc gcgggatttg atgtattgct
     6481 gattccttac ccgttcatcc agtagtgcca gcacggtttc tggtccggcc agaaatttga
     6541 aggcgttgag cgcatcaata tccacaccgt aatctttaag ttcctgttca cttaacaaat
     6601 catcagctgg caacattaac aggcgttcca ttgctggaat tgcacgttcc gccgcctcac
     6661 gcagtgcctg atagttaatt tcgctcactg gccgcctcct ttgcgaagct gggcggcgaa
     6721 ctcgttagcc atcgctgaat atgggtgctc atgaccgcca acaaccttca tgctggcagc
     6781 aaacatctcc acgccctgcg cccgcacttc atccaggaaa gtgtcggtgg ctggggtttc
     6841 tggaatggcg tctggaataa cctctgagta gacgcgatcc attgccgact cccatccgta
     6901 gtaacaagcc gaatagccgt cgcgctggta gccgtggtct tcaactccac atcccatgcc
     6961 ctggtcatgg tattcaggct gattgtcggg attaatgcag tattcaatga ccgacttcat
     7021 ccccgcattc tccgccgcca gcgccgaaaa cttctcgtgt gccaacttaa cagccgcatc
     7081 agcctgctta attgactcaa tcgctttctg gtggtcttcg tacagagccg aaatcttggc
     7141 ctccgcttca gcaaatttac gcaccagata ttcagcgttt gtttcgttaa cctttaaatc
     7201 acatgggatg catttacctt tcagaaaacc atccatctca attagtgaca tttgtttcat
     7261 ttcttcccac tccgccacat agcattcaga tatttgttgt cattaacaga accgaaactc
     7321 tttctcttaa gtaattcctc tctcgatggc attggcttta cgcgttggcg aataatcatt
     7381 tctgccggaa gaatgccggg attgtatgca agtcctctca tggtaaattc ctcagtcatt
     7441 actgatagcg ccatagcgtg agcggtaatt acgcaggcgc gggtcaattt cagggaagtg
     7501 ggtatatgtg gctttgcgga atggtcggat tgatgtctgg taaattcgct cgagttcttc
     7561 tttctctgca agccatatac agtggcgaaa ttccttttcc tctttcgttt cctgcggtag
     7621 cgacattatc cgatcgtagt tttttctgaa tttatccagc acctccgata cggaattgcc
     7681 ggaacagcgg cgtgggtcat ccgcaccata cagaggcgct ggcataatgg aatccttatg
     7741 ttgctacttt agaagggaat tgaatcgtcg tattcaggat gattttgatg attgctactt
     7801 tgctgctgtt ggctgtttcc tgaagttgca aatccaatct ttgcattcag taattcaaga
     7861 gtgattgatt gtccattttg cccctgataa acatcaaccc tgatgttttc tccggtaatt
     7921 tccacaatgc caccttcaac aagaacacta cggtagtaat ccgcttgcgc tcccggcttg
     7981 gcaaatacaa cggcgctgta gtttgtccat tctttctttt ttgtctggcg atcgtaatac
     8041 tgaacgccag cacggatgtt gaatccgata ttttccccgg cctgaaactc tcttgcgggc
     8101 ttgtttagtc ttacagtaat cgaatgtgcc attaagcagc cgctccttct aattcgtctc
     8161 gtctgatgtt gtaaatgtcc tgcgctttgt gctgctccgg tgtgccttcg agcatcttcc
     8221 acgctttggc gaacgcctgt ttaagctctt ccacggtgtt tttctgcaat gctgcgtcag
     8281 tgaatgcttt tagaacctgt tcaggtgtag gtgatggttt tgattgcttt gctgctgcgt
     8341 tctgctgatg tttatgctcg tcggtatctg catctttcgc atcatcaatg ccgaacaaac
     8401 cattgaggca atacttgcgt gcataagagc ttgtggctcc cgtaacttgt gcagaatcca
     8461 ttcctttctt gctttcttcc tctcgtgcaa gagcggttgc cgtatgactg ttttcgccat
     8521 cggtaatagt tgccgtggct ttcacgtaat accgatcacc aatcaacaca acttcatcgc
     8581 tgattgataa aaacagacca ttcagtaacg gcttaacgcc ttcaagaatg tcttcgcagc
     8641 ttctgtattt atatttaccg aatgagttgt actggttctt tggcgcgttc aagttctcct
     8701 gaatagctgc cagtcttgcg taaaattctt tgctcatatg tttgttctca gaatgggcat
     8761 ggcccaagga aataacgctg atttaatact tcgactcggg acaaattaag gcatacccgc
     8821 attccttcgc ggtcgccatt atggcgatac cagagagctt tctgcgtgta catgcgtctc
     8881 tgtaacttgc tctccttcac tgtggttgca agtgacatga atatctcctt cgttaccgat
     8941 taattctttc atctgacgaa tgaattcttc gtctgaccag ttatctgtaa aactcatttc
     9001 ctgcgatacc acggaatatt gatagctgat ttcatcgctt tatttgcttc aagccacatt
     9061 tttgaatcac caataaatct ggctattact gctttgtttt gtgctgcacg aagcatctgg
     9121 tgattgatgg ctatttcatt gcgcataacg cctccagttg tttctttgct gcactgatta
     9181 attgtttaac tcggcgtgat aattcagatt cgtgcgggta gaaagcggac atgacgccgc
     9241 tacccgcgag ctgaaagtgc atcatggata actccttctg tttgattgca taacgaaaac
     9301 gcctcgagtg aagcgttatt ggtatgcata taaaaaagcc ctcatactgg agggcaaaga
     9361 agatttcaaa taatcagaac aagtcggctc ctgtttagtt acgagcgaca ttgctccgtg
     9421 tattcactcg ttggaatgaa tacacagtgc ttactcgtac taataaaata cccaattttc
     9481 tgtttcttgg ttgtgcccaa agttatattc aatatctggt gttgatgtat caatattctt
     9541 catcccatca acaagagttg atacaacagc caaatcctgt ttgattctca ttaaatggta
     9601 tttcttccgg cgcaataaac tttcaatggc aagtttcttc gttgggaatg caaaagatct
     9661 ttctgcattt tttgctactt tcttaattgc atatctattt ctcttttgtt tccattcctg
     9721 taaccactga tttggtgctg gtttaaaatt aacaatccaa tgcgcaggaa ccaaccatgc
     9781 ataatgctct gtctgatgaa aagctatata ttgaagtgcg aatattttga ttccatcttc
     9841 ttcaactgtc gcctggaatc tccagaaaac aggcattcca tcatgttcag tttctgattc
     9901 aggaaaaggt acgctccatg attttgtcat atctcacctc aaataagtgg cttgctgcct
     9961 aatttcattt tctggcgacc aacacaagtc acacccattt cactgcgtgg cttgctgtac
    10021 catgtgcgct gattcttgcg ctcaatacgt tgcaggttgc tttcaatctg ttcgtggtat
    10081 tcagccagca ccgtaaggtc tatcggattc agtgcgcttt ctactcgtga tttcggtttg
    10141 cgattcagcg agagaatagg gcggttaact ggttttgcgc ttaccccaac caacagggga
    10201 tttgctgctt tccattgagc ctgtttctct gcgcgacgtt cgcggcggcg tgtttgtgca
    10261 tccatcttga ttctcctgtc agttagcttt ggtggtgtgg taggtgggag acccatttcg
    10321 acctgcttcg gccgacttca attcggcaat agttccgcag gcctcgccgc tttacgtgcg
    10381 acatattccc atccatgaac ccttcaccac accccaaagc cttctgcttt gaatgctgcc
    10441 cttcttcagg gcttaatttt taagagcctc accttcatgg tggtcagtgc gtcctgctga
    10501 tggcttaaaa ttacaagaaa gattgtatgt tgtaaacaag aaatattgta aaaaggggcg
    10561 taaaaacaaa ctccattgtt tttaaatgaa aaatagtttg tttttggtta tcgagattga
    10621 ggtggggatt actggttgca ggttccgact acatcaccaa caaatgattt ggttgatgta
    10681 agttgttgca tacctgggat attcattact ttggagtaaa gagctttttt gtctgtagtg
    10741 attgatcaag tttcaacggt tattcctcct ccagactggt attctcctac catagtgttc
    10801 gatgacaaag cagtgtattt catctctgga tagacgccag aaactgattc ataaactgat
    10861 gatttatcgc catttattgt tacgtggaaa acggaatctt ccgtgctgtc ttttgtaaac
    10921 tcgtaacgat cgccattcat tgacccgtac ccgtgcaggt ttgtgacaat ccagcattca
    10981 gaattggcgc tggtagttaa gagtattgag agtagcgccg caatcctgat catacgaatt
    11041 ttaccctcgc ttccacgaca acaccgataa tcttgcagtt cccgttgata ggagtcatag
    11101 gccatgaagg gttcaggcct ttcaggtact tctgcccgcc atctataacc agtttcttga
    11161 atgttgcttc gttcgcgtca gtcagtttgg ctacaacaag gcttccattc actggctcgc
    11221 gtccagtatc tactaacacc atatgacctt cagggatgct ttgacctaca ggtgaggtca
    11281 tggaatcacc ttcaaccttc agccagaatc catcgcctaa taagttaacg tcactgtcat
    11341 accattcatc aatgtccttg atatcgtagg gttcacaagc ttcacaccac gaaccagctc
    11401 taaccatgct aatcaatgga tatttccctt tgggctcaac atgcccaaca aatctaacat
    11461 tcgaatcaga ggtgccattg agcagccagt caacacttac gccaagagct gacgcaagtt
    11521 ctggtaaaaa gcgtggtcgc ttagttttac cgttttcgag ctgctctata gactgctggg
    11581 tagtccccac cttttgagca agttcagcct ggttaagtcc aagctgaatt cttttgcttt
    11641 ttaccctgga agaaatactc ataagccacc tctgttattt acctccaatc ttcacaagaa
    11701 aaactgtatt tgacaaacaa gatacattgt atgaaaatac aagaaagttt gttgatggag
    11761 gcgatatgca aactctttct gaacgcctca agaagaggcg aattgcgtta aaaatgacgc
    11821 aaaccgaact ggcaaccaaa gccggtgtta aacagcaatc aattcaactg attgaagctg
    11881 gagtaaccaa gcgaccgcgc ttcttgtttg agattgctat ggcgcttaac tgtgatccgg
    11941 tttggttaca gtacggaact aaacgcggta aagccgctta agacattccc gctcttacac
    12001 atcccagccc tgaaaaaggg cattaccaga aacaaatctc tatggttttg cgtttctttg
    12061 cgaagccaac tctatctaat cattaaggaa attatctatg ggtactattg caactaaaag
    12121 caagaaagcg gctcgcatcg agtcagcctt gctgaacaaa ctggcactga tggggcagaa
    12181 gacattcgct cgagcaatgg gggttcctga atatcaggta agccgatgga agaatggttt
    12241 cttctcgcag gtaagcatga tgcttgctgt tctggaatac ggaatcgaag acgatgaaat
    12301 ggctgaattg actaagcggc ttgccgatta cctgacaaaa gaaaaagccc cgaagaacgg
    12361 cgaattcttc gaggcctaat gtagaaagac tggatcaatc cacgggagta attatgacaa
    12421 aacgtcgtaa gaaataccag gaaaaagaag agattcgaca ccctgattca cctgagggat
    12481 tagtggtagc cgcagcaaat aacagggcgt tcgcagagcg ccttgttggt gtttacagac
    12541 tagccaaagc aggagtgaaa catgggcgtc gttaagttag ctgattacag gcctcaactg
    12601 gaggtcgtgg agcatcgcgt ggcagaactc gaagatggct acactcggac tgcaaacaca
    12661 ctgttagaag ccgccatgct ttctggactt actctacatc agttactgat tgttatggct
    12721 gtgtggcgca agacatacgg ttacaacaaa aaaatagatt ggatcggaaa cgaacagttc
    12781 gctgaactca ctggcatggc accaaccaaa tgctctaccg ccaaaaacga gcttatcaga
    12841 atgggagttc tcattcaggt ggggcgtcaa gttggtatga atacaaacat ttccgagtgg
    12901 aaaacgaaag ttaacggatt cggtaaaaca tttaccaatt cggtaaaaca aaccttcacc
    12961 aaatcggtaa aaagcaattt accgaatcag tcaaacacaa aagacaatat acaaaagaca
    13021 ataaatacaa ataccccctt accccctaac gggggcggcg atgggcaggt taaacctgaa
    13081 cgtcgcaagg cagaacgaat cgactacgaa tccttcctga acgcctacaa caccgaagtc
    13141 ggtgacagac tgccacacgc tgttgcggtc aacgagaaac gcaaacgccg cctgaagaaa
    13201 atcatcccgc aactgaaaac gccaaacgtg gacggtttca gagcgtatgt cagggcgttt
    13261 gtgcatcagg ccaagccgtt ttacttcgga gacaacgaca cgggctggac ggccgatttt
    13321 gattacctgc tgagggaaga ctcgttaacg ggggtacggg aagggaagtt tgcagacagg
    13381 gggattgcat gaaacaggat atcgaagcga gcgttatcgg tggcctgctg attggtggat
    13441 taacaccaac cgccagcgac gttctggcaa cgctggagcc ggaagcgttt tcaattccgc
    13501 tctaccggaa agccttcgag gttatccgca agcaggcgcg aaacagaaac ctaatcgacg
    13561 cgctgatggt tgccgaggcg tgcggagagg agcatttcac gtcaatcctg atgaccagca
    13621 agaactgccc gagtgccgca aacctgaagg gatatgctgg aatggtcgcg gataactatc
    13681 accgccgtct ggtgctggaa atcatggatg aaatgcgtga accaatccaa agcggaacca
    13741 tcgacgcatc gagtcaggcg atggatgaac ttgtaaagcg tctttcagcc atcagaaagc
    13801 cccgtgacga ggtaaaacct gtacggttag gggaaatcat cactgactac actgacacgc
    13861 ttgacaggcg tctgaggaac ggagaagagt cagataccct gaagaccgga atcgacgaac
    13921 ttgacgccat caccggaggg atgaacgcag aagacctggt gattatcgcc gctcgtcctg
    13981 gtatgggtaa aaccgaactg gcgctgaaga ttgccgaagg cgttgcaagc cgcgttattc
    14041 ctggttctga cgtccggcgc ggagtgttga ttttctcgat ggaaatgagc gcattgcaga
    14101 ttgcagagcg gagcattgcc aacgccggga ggatgtcggt tagcgtgctg cgaaatcctg
    14161 catcgatgga tgacgaaggc tgggcgcgtg ttgctaacgg catgagtcag cttgcagatt
    14221 tggatgtatg ggtagtcgat gcctcgcggt tatcggtcga agaaatacgc tcaatcgcag
    14281 aacggcacaa acaggaaaat ccaaacctgt cactcatcat ggcggattat cttggcctga
    14341 ttgagaagcc gaaagcagac cgcaacgacc tcgcaattgc tcacatctcc ggaagcctga
    14401 aggcgatggc gaaagacctg aaaacgccag ttatctccct gagtcagctt tcgcgcgatg
    14461 ttgagaagcg accaaacaaa cgcccgacaa acgcagattt gcgtgattca ggaagcattg
    14521 aacaggacgc agactcaatc atcatgctct atcgggaagc ggtatatgac gagaacagta
    14581 gcgccgcgcc atttgctgaa atcatcgtga cgaaaaaccg ttttggctcg cttggtacgg
    14641 tttaccagcg gttctgtaac ggacactttg ttgcatgtga ccaggatgaa gccagacaga
    14701 tttgcacagc atcaaatgca cctgctgcgc gtggcagacg atatgcacaa ggggctgacg
    14761 tatgaccatc tacatcactg agataataac aggcctgctg gtaatcgcag gcctttttat
    14821 ttgggggaga gggaagtgtg gctgactggc aaattccaat catcattctt gccggagctt
    14881 cgctggttgc tggctttatc ctgctgaaaa agcataaaga ccgtgatcaa aaagttgaag
    14941 ttctctatgg gtatccagcg aacagcacaa catggctgac catttaccac taccgaaaat
    15001 caggacgctg ggtattcgaa tgggatgatc tgttcgctga aaagcgacca aagtcatggg
    15061 gagacatcag cgaatgcatg atgtttgaag aaagaaaatc cggcgcaacc cgagaagagt
    15121 ttaacgaagc gtgggcgcga ttaagtgaga gagggtattt gtgagcaagt acgaaaaatt
    15181 agatcaaaac attctttcaa tgctgagtga aagaccaaca cctgtttttg atatctggct
    15241 taaatggcgg agcaatggaa tgtatatcga aaccatcgat cgccgtatgc aatacctgag
    15301 aaagaaaggg cttgttgcaa atgtgcgtgg gaagggttgg gtgaaaatta acctgtcata
    15361 acggggattg atatggacga tagcttagaa atatttgaag agtggtgcgc tgatgaactt
    15421 ggtgtctccg cttacttcat tcgacaaatg cgaagcaaga atattttggg cgtgattgag
    15481 tataaacgag ttgaaataaa caagagatac agggcttgga tggccgcagt tcgcgccgcc
    15541 ggagtcaaag tgaaggagtg agtatgagcg catacgaaga aatcatgtta gccctgcgat
    15601 tctttttcgg tgtggaagaa gatgaaaacg taaatgagat tatcggacaa gaccatgacc
    15661 cgatagggac tattgcagct gcacttgacg attacaggag cgtaaatggt gaggaaacta
    15721 acgtttgaac taagaagccc catccatcag cagaacgcca ttcaagctat ccagcaaatc
    15781 cttccagacc caaccaaacc aatcgtagta accattcagg aacgcaaccg cagcttagac
    15841 cagaatcgaa agctttgggc ttgccttggt gacgtttcgc gtcaggttga atggcatggt
    15901 cgctggctgg atgcagaaag ctggaagtgt gtgtttaccg cagcattaaa gcagcaggac
    15961 gttgttccta accttgccgg gaatggattt gtggtaatag gccggtcaac cagcaggatg
    16021 cgtgtaggcg aatttgcgga gctattagag cttatacagg cattcggtac agaacgtggc
    16081 gttaaatggt cagacgaagc gcgactggct ctggagtgga aagcgagatg gggagataaa
    16141 actgaatgaa caaataccga cttatttacg cagatccgcc ttggcaatat cgcgacaaag
    16201 ccaacgatgg caatcgcggt gctggacata aatacgatgt tatgaatgtt caggacattt
    16261 gccgactgcc agtatgggat ttagcggatc cagaatcttg cttgttagcg atgtggtggg
    16321 tgccgacaca gccagccgaa gcgctaaagg taattgaggc gtggggattc aggttgatga
    16381 ctatgaaagg ctttacttgg cacaaaacca ataagcacaa aggcaacagt gcgatcggaa
    16441 tggggcatat gaccagggca aatagcgagg attgcttgtt tgccgttcga gggcggttac
    16501 ctgagagaat ggacgcttca atatgccagc actttaccgc accgagaatg gagcacagtg
    16561 caaaaccacc gatcgtaaga gacatgttag ctaagttgct tggcgacgtg ccgcgctgtg
    16621 agttatttag ccgcgacaaa gtgaccgggt gggatatgtg gggaaatcag tgcgactccg
    16681 attttgaact tgctcccggc atggcgatta aaccatgcaa aatggtgatc gcatgaagca
    16741 ctgttatcga tgtggagagc gaaaggaaga cgatcgcttt cgaccaggac agccttactg
    16801 gaatcgatgg tgtctccggt gtgaaagaac accaacaggg gtgttaccac taccgcagga
    16861 aaaggaggac gtgtggcgag acagcgacga agtatcaccg acataatctg tgaaactgca
    16921 aataccttcc aacgaaacgc tccagaaata aacgcaagcc aatcccaaaa gaatctgacg
    16981 taaaaacctt caactacacg gctcacctgt gggatatccg gtggctaaga catcgtgcga
    17041 ggaaatgaca atgcttttaa ttcaacctgg atttggactt agcatcaaaa aaggccacat
    17101 gtttggcgag aaagagtcac aacgaaaaat gatgtctatc cggttgccat ttatcagtat
    17161 ttattggcta aacagggagg caacaaatta ttggtataca tgcgccagag cagcatttaa
    17221 cgaccctgac tggtttgtgg aaaaccacca cgcagttcgt caggcaaaga gaaaggccaa
    17281 catgacatac atgaaggcgt atcgaaaagc atggaaagaa caccgcgatc gataccagca
    17341 agatatggaa aagcttgaat cagaaaacat ggaattaaga cgaaagcttg gtgaagcaaa
    17401 acgagacatt gatgcttaca agcgactttt taatggtgaa agccatgctt agtccatccc
    17461 aatctcttca ataccagaaa gaaagcgtcg agcgggcttt aacgtgcgct aactgcggtc
    17521 agaagctgca tgtgctggaa gttcacgtgt gtgagcactg ctgcgcagaa ctgatgagcg
    17581 atccgaatag ttcaatgtac gaggaagaag acgatggcca aaccagcgcg aagacgatgt
    17641 aaaaacgaag aatgtcggga atggtttcac cctgcattcg ctaatcagtg gtggtgctct
    17701 ccagagtgtg gaaccaagat agcactcgaa cgacgaagta aagaacgcga aaaagcggaa
    17761 aaagcagcag agaagaaacg acgacgagag gagcagaaac agaaagataa acttaagatt
    17821 cgaaaactcg ccttaaagcc ccgcagttac tggattaaac aagcccaaca agccgtaaac
    17881 gccttcatca gagaaagaga ccgcgactta ccatgtatct cgtgcggaac gctcacgtct
    17941 gctcagtggg atgccggaca ttaccggaca actgctgcgg cacctcaact ccgatttgat
    18001 gaacgcaata ttcacaagca atgcgtggtg tgcaaccagc acaaaagcgg aaatctcgtt
    18061 ccgtatcgcg tcgaactgat taaccgcatc gggcaggaag cagtagacga aatcgaatca
    18121 aaccataacc gccatcgctg gactgtcgta gagtgcaagg cgatcaaggc agagtaccaa
    18181 cagaaactca aagacctgcg aaatagcaga agtgaggccg catgacgttc tcagtaaaaa
    18241 ccattccaga catgctcgtt gaagcatacg gaaaccagac agaagtagca cgcagactga
    18301 aatgtagtcg cggtacggtc agaaaatacg ttgatgataa agacgggaaa atgcacgcca
    18361 tcgtcaacga cgttctcatg gttcatcgcg gatggagtga aagagatgcg ctattacgaa
    18421 aaaattgatg gcagcaaata ccgaaatatt tgggtagttg gcgatctgca cggatgctac
    18481 acgaacctga tgaaaaaact ggagacgata ggattcgaca ccaaaaaaga cctgcttatc
    18541 tcggttggcg atttggtcga tcgcggtaca gagaacgtcg aatgcctgga attaatcaca
    18601 ttcccctggt tcagagctgt acgtggaaac catgagcaaa tgatgattga tggcttatca
    18661 gagcgtggaa acgtcaatca ctggctactt aatggcggcg gctggttctt taatatcgat
    18721 tacgacaaag aaattctggc taaagctctt tcccataaag cagatgaact tccgttaatc
    18781 atcgaactgg tgagcaaaga taaaaaatat gtcatctgcc acgccgatta tccttgtgac
    18841 gaatacgagt ttggaaagcc agttgatcat cagcaggtaa tctggaaccg cgaacgaatc
    18901 agcaactcac aagacgggat cgtgaaagaa atcaaaggcg cggacacgtt catctttggt
    18961 catacgccag cagtgaaacc actcaaattt gccaaccaga tgtatatcga taccggcgca
    19021 gtgttctgcg gaaacctcac attgattcag gtacagggag aaggcgcatg agactcgaaa
    19081 gcgtagctaa atttcattcg ccaaaaagcc cgatgatgag tgactcacca cgggccacgg
    19141 cttctgactc tctttccggt actgatgtga tggctgctat ggggatggcg caatcacaag
    19201 ccggattcgg aatggctgca ttctgcggta agcatgaact cagccagaac gacaaacaaa
    19261 aggctatcaa ctatctgatg caatttgcac acaaggtatc ggggaaatac cgtggtgtgg
    19321 caaagctcga aggaaatact aaggcaaagg tactgcaagt gctcgcaaca ttcgcttatg
    19381 cggattattg ccgtagtgcc gcgacgcctg gcgcaagatg cagagattgc cacggtacag
    19441 gtcgtgcggt tgatatagcc aaaacagagc agtgggggag agttgtcgag aaagagtgcg
    19501 gaagatgcaa aggcgtcggc tattcaagga tgccagcaag cgcagcatat cgcgctgtaa
    19561 cgatgctaat cccaaacctt acccaaccca cctggtcacg cactgttaag ccgctgtatg
    19621 acgctctggt ggtgcaatgc cacaaggaag agtcaatcgc agacaacatt ttgaatgcgg
    19681 tcacacgtta gcagcatgat tgccacggat ggcaacatat taacggcatg atattgactt
    19741 attgaataaa attgggtaaa tttgactcaa cgatggataa atgcactcgt taaataaagc
    19801 cctgagttaa tagctcgggg ctttttgcgt tttaagcacg gcctttctga aagcacatca
    19861 agccaaatac cagacagaca aaaataatca ccttatccgc ttggctacgg tgcggtgtgc
    19921 tttgcataaa agaaaaccag cgcaatggct ggcttcgtga aagcgggtgg caagaggctg
    19981 cgctaacaac ctcatgccgt tttgcccgtg catatcggtc acgaacaaat ctgattacta
    20041 aacacagtag cctggatttg ttctatcagt aatcgacctt attcctaatt aaatagagca
    20101 aatcccctta ttgggggtaa gacatgaaga tgccagaaaa acatgacctg ttagccgcca
    20161 ttctcgcggc aaaggaacaa ggcatcgggg caatccttgc gtttgcaatg gcgtaccttc
    20221 gcggcagata taatggcggt gcgtttacaa aaacagtaat cgacgcaacg atgtgcgcca
    20281 ttatcgcctg gttcattcgt gaccttctcg acttcgccgg actaagtagc aatctcgctt
    20341 atataacgag cgtgttcatc ggctacatcg gtactgactc gattggttcg cttatcaaac
    20401 gcttcgctgc taaaaaagcc ggagtagaag atggtggaaa tcaataatca acgtaaggcg
    20461 ttcctcgata tgctggcgtg gtcagaggga acagataacg gacgacagaa aaccagaaat
    20521 catggttatg acgtcattgt aggcggagag ctatttactg attactccga tcaccctcgc
    20581 aaacttgtca cgctaaaccc aaaactcaaa tcaacagcag ccgggcgcta tcagcttctt
    20641 tcccgttggt gggatgccta ccgtaagcag cttggcctga aagacttctc tccgaaaagc
    20701 caggacgctg tggcattgca acagattaaa gagcgtggcg ctttgccgat gattgatcgc
    20761 ggtgatatcc gtcaggcaat cgaccgttgc agcaatatct gggcttcgtt gcccggggct
    20821 ggttatggcc agttcgagca taaggctgac agcctgattg caaaattcaa agaagcaggc
    20881 ggaacggtca gagagattga ggtatgagca gagtaaccgc gattatctcc gctctggtta
    20941 tctgcatcat cgtctgcctg tcatgggctg ttaatcatta ccgtgataac gccatcgcct
    21001 acaaagagca gcgcgacaaa gccgcatcca ctatcgctga tatgcagaag cgtcaacgtg
    21061 acgtagcaga actcgacgcc agatatacaa aggagcttgc tgatgctaac gcgactatcg
    21121 aaagcctccg tgctgatgtt tctgctggtc gtaagcgcct gcaagtcgcc gccacctgtg
    21181 caaagtcaac gaccggagcc agcagcatgg gcgatggaga aagcccaaga cttacagcag
    21241 atgctgaact caattattac cgtctccgaa gcggaatcga caggataacc gcgcaggtta
    21301 actacctgca ggaatacatc aggactcagt gcctgaaata atttttttgc aaatcacaaa
    21361 gtccatttaa tgagcctcgc gatgcggggc ttttttacat ctgaatttca cagcgcatct
    21421 cacgcgcata ttacatcacc cgagcctttc agaaagttga gcctgagaac tgccgtatat
    21481 ggtggcgacc atctcgggac ggcttttctg tgagacaggc tcactttcta aaaggtaaag
    21541 acgcaatgaa ctacccaacc gttgttaacg atatagattt cagagaccta atttttgtag
    21601 caaacaacga tccggttaca gattctttta tggtggcaaa agcatttgga aagctgccga
    21661 agaacgtagt tcgtgacatt gagcgaacca tagaagcttg ccctcctgag tttgatacaa
    21721 agctcaactt tgagctttgc tataaaaaca atgagttaca gaatggtaag ccgcaaaaat
    21781 tctaccgtct ccgcaaggat gggttgatgc ttttggttat gtccgacacc aaaaaagaag
    21841 caatgcgtat caaaattgct tacatcaacg catttaactg gatgtacgcc atgcttcagg
    21901 ttggtcatcg tcaatttgaa gaagagagaa atgccgtaat gctggagtac atgaaagaga
    21961 aggatgttgc cagcatgtca ggtcgcctgc taaatcgctg gggcaaaatt aagaagccac
    22021 agctgatggc tagaattgaa cgccttgaac agcacgggca aaccgtaatc cccggactca
    22081 tcaattaacg gcagcaccgc gaaacaaccc aagccagtaa gtggggaaat aacactggca
    22141 gccactgaaa gatgaacctc cagccttatg gcaaaaaaga ttctttgtgg tggcggactg
    22201 atggaaagac atcggataga atcaagcagt ggctagggta gctcccgaaa agcggactcg
    22261 tcaccgcctg ccactgaatc tatggcgaac aactagacga ggttgtgatg gatgataacg
    22321 gaaaccaatg ggtcaatgtt gaacaccggc taccagaaag caaagaaggg atgtggtcta
    22381 aagaggttat agctcttact gatactggtg acgtgttcaa actatcatgt atgggctctt
    22441 actggcagag aaccaaggca ttcatcgatt caggagcaag caaggttaca cactggatgc
    22501 cgcttaatta cccagatgat taaaacggat gaaaaatgaa aggtcgctca ggcggccttt
    22561 tttattgcca tcacaaaagc cattccttac tgagtggctt tgataatggc ttatacccta
    22621 cacgggataa cttaactgat atccctttta acggataaat ggagccaaca atggcagaga
    22681 ttattcccat gactgaagaa cagaaattcc agttagagat ttacaaactg gtcatgaacc
    22741 agaacgcagc cgcagaagaa gcatttcaat tcattggcac tgacgaactg aagcttgagc
    22801 tattcaaaat tcacttccag tcaggtggcg ctaattcaga tatcacgatc cgcacatttg
    22861 aagcggtgcg taaatcgaag gaagcgttag acctgttcac caccggagca tgatgctcaa
    22921 cctgaaataa cgattaagtg agatgaatat ggcagcacca aagggcaacc gattctggga
    22981 ggcccgcagt agtcatgggc gaaaccctaa attcgaatcg cctgaggcgc tgtgggctgc
    23041 ttgttgtgaa tacttcgagt gggctgatga taacccgcta tgggagggta aggtattttc
    23101 atatcaggga gaaataatta aggctaatgt ccctaagatg cgagccatga ctatttcagg
    23161 attgtgtacc ttccttgata tcaccaggca aacatgggga accttccggt caatggaagg
    23221 tttttctgac gtcacatcac gagcggaaga catcatctac gaccagaaat tctctggcgc
    23281 agccgctgac cttctcaacg ctaacatcat cgcccgtgat ttgggcctca aagagcagtc
    23341 gcaagttgaa gacgtgacac ctgataaggg agatcgcgat aagcggcgct ctcgtatcaa
    23401 ggagctattc aaccgtggaa ctggacgcga ttcttgataa cttgagcgac gaagagcaaa
    23461 tcgaattgct cgagctactc gaagaagaag agaactaccg taacacacac ctgctatatg
    23521 aatttacgcc atacagcaaa cagcgtgagt tcatcgacgc cgggcatgac tatccagagc
    23581 gctgttttat ggctggtaac cagcttggta agtcatttac tggtgctgct gaagtcgcgt
    23641 ttcaccttac cgggcgttat ccgggcacaa aaggctatcc tgctgatggt aaatatggcg
    23701 gtgagtggaa aggtaagcgt ttttatgagc ctgttgtctt ttggattggt ggcgagacaa
    23761 acgagactgt aaccaaaacg actcaacgca tcctgtgtgg tcgtatcgaa gagaatggtg
    23821 agccaggcta cggttccata cctaaagaag acatcattag ctggaagaag tctcctttct
    23881 ttccgaacct tgttgatcac cttctggtta agcatcacac ggctgatggc gttgaagatg
    23941 gcatttcaat ctgctacttc aagccatact cgcaaggtcg tgctcgctgg cagggtgaca
    24001 caatccacgg cgtgtggttt gacgaagagc caccatacag catttatggt gaaggcctta
    24061 cccgtaccaa caaatacggg caattctcaa ttctgacgtt taccccgctg atggggatgt
    24121 ctgacgttgt taccaagttc ctgaagaatc caagcaagtc gcagaaagtg gtcaacatga
    24181 ccatctacga cgctgagcac tacacagacg aacagaaaga gcaaatcatc gcatcttatc
    24241 ctgagcatga gagagaggcg cgtgctcgcg gtattcctac gatgggtagt ggtcgaatct
    24301 tccagatacc ggaagagacg attaagtgtc agccgttcga gtgtcctgat cacttctacg
    24361 taattggcgg gatggatttc ggatgggatc acccgcaggc gcaggttcag ctttggtggg
    24421 acaaggacgc agacacaata tacgtttcac gcgtgtggaa ggcgaaagaa aaaacagccg
    24481 ttcaggcgtg gggagccgtt aaatcatggg cgcataaagt gccaactgca tggcctcatg
    24541 acggaaacca gcacgagaag ggcggcggtg agcagctcaa agggcagtat gcagatgctg
    24601 gttttatgat gttgcaggag catgcgacat ggcctgatgg cggtaatgct gtggagcctg
    24661 gcatcactga attgcgcgac atgatgctcg atggtcgctt caaagtattc aacacctgtg
    24721 agccattctt tgaggagttc cgcctctatc accgtgatga aaacgggaaa atcgtcaagc
    24781 ttaacgacga cgttctctca gccgttcgct atgcatacat gatgcgccgc ttcgccaaaa
    24841 tgatgcgcga catcaaaaaa ccaaaagaga aaaagatacc agccccaatc aggcccatcg
    24901 cacggagaac ttaaatggcc gacgaaaaca gactcaattc cattctgtgt aagtttgacg
    24961 c
//
LOCUS       DAZOZX010000050        24653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZOZX010000050 DAZOZX010000000
VERSION     DAZOZX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.050"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..386)
                     /locus_tag="ACHUPT_002009"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHUPT_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHUPT_002010"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHUPT_002010"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0819591.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHUPT_002011"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHUPT_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0819592.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHUPT_002012"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHUPT_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0819593.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHUPT_002013"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHUPT_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0819594.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHUPT_002014"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHUPT_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0819595.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHUPT_002015"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHUPT_002015"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0819596.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHUPT_002016"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHUPT_002016"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0819597.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHUPT_002017"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHUPT_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0819598.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHUPT_002018"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHUPT_002018"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0819599.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHUPT_002019"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHUPT_002019"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0819600.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHUPT_002020"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHUPT_002020"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0819601.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHUPT_002021"
     CDS             10748..11212
                     /locus_tag="ACHUPT_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0819602.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHUPT_002022"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHUPT_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0819603.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHUPT_002023"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHUPT_002023"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0819604.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHUPT_002024"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHUPT_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0819605.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHUPT_002025"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHUPT_002025"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0819606.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHUPT_002026"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHUPT_002026"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0819607.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHUPT_002027"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHUPT_002027"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0819608.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHUPT_002028"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHUPT_002028"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR0819609.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACHUPT_002029"
     CDS             complement(20505..20798)
                     /locus_tag="ACHUPT_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0819610.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACHUPT_002030"
     CDS             complement(20795..22084)
                     /locus_tag="ACHUPT_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0819611.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACHUPT_002031"
     CDS             complement(22140..23078)
                     /locus_tag="ACHUPT_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR0819612.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACHUPT_002032"
     CDS             complement(23098..23862)
                     /locus_tag="ACHUPT_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR0819613.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24181..>24653
                     /locus_tag="ACHUPT_002033"
     CDS             24181..>24653
                     /locus_tag="ACHUPT_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR0819614.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5950 c         6484 g         6116 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gacaatgaaa ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    24361 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    24421 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    24481 tttcaggggg ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg
    24541 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    24601 agtaaaaata gccaaacatc ttcgttatgg gatgcattag cctatacatt agc
//
LOCUS       DAZOZX010000051        24454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZOZX010000051 DAZOZX010000000
VERSION     DAZOZX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.051"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..202)
                     /locus_tag="ACHUPT_002034"
     CDS             complement(<1..202)
                     /locus_tag="ACHUPT_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR0819615.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLAPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGM"
     gene            complement(295..867)
                     /locus_tag="ACHUPT_002035"
     CDS             complement(295..867)
                     /locus_tag="ACHUPT_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0819616.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            1085..1945
                     /gene="tesB"
                     /locus_tag="ACHUPT_002036"
     CDS             1085..1945
                     /gene="tesB"
                     /locus_tag="ACHUPT_002036"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0819617.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1994..3280)
                     /gene="amtB"
                     /locus_tag="ACHUPT_002037"
     CDS             complement(1994..3280)
                     /gene="amtB"
                     /locus_tag="ACHUPT_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0819618.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3310..3648)
                     /gene="glnK"
                     /locus_tag="ACHUPT_002038"
     CDS             complement(3310..3648)
                     /gene="glnK"
                     /locus_tag="ACHUPT_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0819619.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3829..5610)
                     /locus_tag="ACHUPT_002039"
     CDS             complement(3829..5610)
                     /locus_tag="ACHUPT_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0819620.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5603..7375)
                     /locus_tag="ACHUPT_002040"
     CDS             complement(5603..7375)
                     /locus_tag="ACHUPT_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0819621.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7405..7863)
                     /gene="decR"
                     /locus_tag="ACHUPT_002041"
     CDS             complement(7405..7863)
                     /gene="decR"
                     /locus_tag="ACHUPT_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR0819622.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(8017..8835)
                     /gene="cof"
                     /locus_tag="ACHUPT_002042"
     CDS             complement(8017..8835)
                     /gene="cof"
                     /locus_tag="ACHUPT_002042"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0819623.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8935..10627
                     /locus_tag="ACHUPT_002043"
                     /pseudo
     CDS             8935..10627
                     /locus_tag="ACHUPT_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10692..11387
                     /gene="queC"
                     /locus_tag="ACHUPT_002044"
     CDS             10692..11387
                     /gene="queC"
                     /locus_tag="ACHUPT_002044"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0819624.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11439..11837)
                     /gene="fadM"
                     /locus_tag="ACHUPT_002045"
     CDS             complement(11439..11837)
                     /gene="fadM"
                     /locus_tag="ACHUPT_002045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0819625.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11931..12302)
                     /locus_tag="ACHUPT_002046"
     CDS             complement(11931..12302)
                     /locus_tag="ACHUPT_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0819626.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12453..14324)
                     /gene="ppiD"
                     /locus_tag="ACHUPT_002047"
     CDS             complement(12453..14324)
                     /gene="ppiD"
                     /locus_tag="ACHUPT_002047"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0819627.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DVASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14516..14806)
                     /gene="hupB"
                     /locus_tag="ACHUPT_002048"
     CDS             complement(14516..14806)
                     /gene="hupB"
                     /locus_tag="ACHUPT_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0819628.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14997..17351)
                     /gene="lon"
                     /locus_tag="ACHUPT_002049"
     CDS             complement(14997..17351)
                     /gene="lon"
                     /locus_tag="ACHUPT_002049"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0819629.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17539..18813)
                     /gene="clpX"
                     /locus_tag="ACHUPT_002050"
     CDS             complement(17539..18813)
                     /gene="clpX"
                     /locus_tag="ACHUPT_002050"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0819630.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18939..19562)
                     /gene="clpP"
                     /locus_tag="ACHUPT_002051"
     CDS             complement(18939..19562)
                     /gene="clpP"
                     /locus_tag="ACHUPT_002051"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0819631.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19809..21107)
                     /gene="tig"
                     /locus_tag="ACHUPT_002052"
     CDS             complement(19809..21107)
                     /gene="tig"
                     /locus_tag="ACHUPT_002052"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0819632.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21451..21768)
                     /gene="bolA"
                     /locus_tag="ACHUPT_002053"
     CDS             complement(21451..21768)
                     /gene="bolA"
                     /locus_tag="ACHUPT_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0819633.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            22073..22651
                     /locus_tag="ACHUPT_002054"
     CDS             22073..22651
                     /locus_tag="ACHUPT_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0819634.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22695..24170
                     /gene="ampG"
                     /locus_tag="ACHUPT_002055"
     CDS             22695..24170
                     /gene="ampG"
                     /locus_tag="ACHUPT_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0819635.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5747 a         6731 c         5898 g         6078 t
ORIGIN      
        1 ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg cgtagcgtta
       61 cagtacctga ttcggcaagg caacgagaga tgagttcggg ggcgaggtaa tcccccagag
      121 aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc tgtccaatgt
      181 gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc actggatcgg
      241 tcaactgatc cttaactgat cggcattact ccgggcggag agggttaacg tcgcttaata
      301 ttgcgtagga gcgggaactg ccgaagacgg attcacctga gttggtgaag tcgaaggtac
      361 ggtagtcgtt gcgccaccgc tggcctgaac cggcacggcg gtttgctgta ccggcaccaa
      421 tgtcaggtcg gctttagttc cgccctggtt gatcaccggc tgaacggtat cggtgataaa
      481 taccagtttg tcattcacgg taatcgccgc actcaacaga atacgcgcgt tcggctgaac
      541 atctgccggg ttaaatggca gaacaaagct gaatggtgac tgtttacctt cagtacgcac
      601 cgctttctgc gccagcactt ttgacggtgc atcggctaac gacgcgtcag aaagtgtcac
      661 ggtcagcaca gcatcaggcg gcagtgcgac tttctgacgg atccagacag taccggagac
      721 attcggttgc tggatagcag gttgttgtgt tgctgaaata gacgtatttg cagccggggc
      781 tggcgtctga atatccgcgc ttttatctgc gcaagccgcc aacgcaatcg caaccgctaa
      841 accactggcc atgtgcacga gtttcattca tttctcctta ttatcaatgc accagcgggc
      901 taactttcct cgccggaaga gtggttaaca aaatagtaac gtcaacaagt gtggcacaca
      961 tcacgcattt ctgcctgtaa ttagcccgta attcagacta tttcacccat cggaccagtg
     1021 gcaaatttgc gttatactca actcactttg gcttgctgcg gcagctttgt tactggagag
     1081 ttatatgagt caggcgctaa aaaatttact gacattgtta aatctggaaa aaattgagga
     1141 aggactcttt cgcggccaga gtgaagattt aggtttacgc caggtgtttg gcggccaggt
     1201 cgtgggtcag gccttgtatg ctgcaaaaga gaccgtcccg gaagagcggc tggtacattc
     1261 gtttcacagc tactttcttc gcccaggcga tagtaagaaa ccgattattt atgatgtcga
     1321 aacgctgcgt gacggtaaca gcttcagcgc ccgccgggtt gctgctattc aaaacggcaa
     1381 accgattttt tatatgacgg cctctttcca ggcaccagaa gcgggtttcg aacatcaaaa
     1441 aacaatgccg tccgcgccag cgcctgatgg cctgccttcg gaaacgcaaa tcgcccaatc
     1501 gctggcgcac ctgctgccgc cagtgcttaa agataaattc atctgcgatc gtccgctgga
     1561 agtccgtccg gtggagtttc ataacccact gaaaggtcac gtcgcagaac cacatcgtca
     1621 ggtgtggatc cgcgcaaatg gtagcgtgcc ggatgacctg cgcgttcatc agtatctgct
     1681 cggttacgct tctgatctta acttcctgcc ggtagctcta cagccgcact gcatcggttt
     1741 tctcgaaccg gggattcaga ttgccaccat tgaccattcc atgtggttcc atcgcccgtt
     1801 taatttgaat gaatggctgc tgtatagcgt ggagagcacc tcggcgtcca gcgcacgcgg
     1861 ctttgtgcgc ggtgagtttt atacccaaga cggcgtgctg gttgcctcga ccgttcagga
     1921 aggggtaatg cgtaatcaca attaaaaaaa tagccggagg tgaaaaccgt ccggctattt
     1981 tttgcagtgc ttgttacgcg ttataggcat tctcgccgtg gctgttgacg tccagccctt
     2041 ctcgctcttg ttcttccggc acacgtaggc caaccgtcag atccgccagt ttgtagccaa
     2101 taaatgccac cacaccggac cagacgatcg taatggcgat gctttccaac tgtaccagca
     2161 actgatggcc catcgtcaca ccttcagcga agcccacgcc gcccagagaa ctggcggcaa
     2221 aaatcccggt catgatacag ccgacaatgc cacaaacgcc gtgcacaccg aagacatcgc
     2281 agggatcatc cacccgcagc aagcgtttga gcatggtaac gccccacaaa cccgccagac
     2341 cagctaccac gccgataatc aacgcgccgc caaccccaat gtagccgcac gctggcgtca
     2401 cgccgaccag accggcaatc gcgccagaac acgctcccag cagagaaggc ttaccacgca
     2461 gcgcccattc accgaagatc cagccaagaa ttgccgccgc cgttgcgacc acagtattca
     2521 caaatgccag tgccgcgatt tcattcgccg tgcccactga cccggcgtta aagccaaacc
     2581 aaccgatata gagaatggca gtcccggtga agaccatcgg caggttgtgc ggtttaaacg
     2641 cctctttacc gaagcctacg cgtttgccta tcagatacgc gcccaccaga ccggcgattg
     2701 ctgcgttaat gtgcaccacc gtgccacccg cgaaatccag cgcaccgtga gaagccagca
     2761 aaccaccgcc ccacaccata tgcgcaatcg gaatgtaaga gagcgtcagc cataccacca
     2821 cgaaaatcaa cacagctgag aagcggattc gttccgccag cgccccaact atcaagccta
     2881 cggtaatgca ggcaaacgat ccctgaaacg ctacgtggat atactgataa atgctgccca
     2941 tcaccgccgt cagttcgatg ttttttagca tcaaccagtt aatgttgccg aagaagttgt
     3001 tgccctcacc aaacgccagc gaataaccgt aaaccaccca gagaatacag accagtgcaa
     3061 acgtcaccgt cacctgcgtc agcatcgaca gcacgttttt gccgcgaatc aacccaccgt
     3121 aaaacagggc aatccccgga atagtcataa acagcaccag cgcagtacaa atcatcataa
     3181 acgcattgtc ggctttatcg gccaccgcag gtgcagccat taccagtccc ggcagcatcg
     3241 ccagtgaagc aagcccagtt tttatcgtcg ctatcttcat ttttcgttcc tgttgctgtg
     3301 tgccagagat tacagcgccg cttcgtcggc ttcgccggta cgaatacgaa tgacgcgttg
     3361 caattcagcg acgaagattt tgccgtcgcc aatttttccg gtgtaagccg ccttactgac
     3421 gatatcgatc acttcatcga gttggtcatc agcaatcgcc acatcaattt ttacttttgg
     3481 caggaagttg acgctgtatt ccgccccccg gtacagctcg gcatgccctt tctgacgccc
     3541 gaaacctttc acttcggtga cggtcaggcc ctgaatacca atggaagata acgcttcacg
     3601 aacgtcttcc agcttgaatg gtttgattat cacggtcacc agcttcatag atcccctccg
     3661 gtcagaattc ggtaatggtt ctgctacacg aagaaggtat tgcaagcggt gtgccagaaa
     3721 tgaaaaacga gaaagagagc aggaagttaa gaagattgaa aatgaaaacg cactatgaca
     3781 gtgcacagtg cgttacattt ttgcaccaac gatgaaatat tgcgctattc aggcgctcaa
     3841 tgattcctct tcacgcacgc tggctgccag ctcttcgccc gcaagttgca gttgatacat
     3901 ctgccagtag cgtccctggg ccgccagcag ttgctggtga gtgccctgct ccacggcttg
     3961 cccacgatga agcaccagaa tggtgtcggc atcaataata gttgataagc ggtgagcaat
     4021 caccaccagc gtggtatgtt cacgcaccgc cgccagagca tgttgaatcg cctgttcagt
     4081 accggagtca atgctggcgg ttgcctcatc aaggatcagg atttgcggcg tctccaccag
     4141 cacgcgcgcc agtgccagca gttgcttttg cccgactgag agattatttc cctgctcgcc
     4201 cagcggcgta taaatacaat cactcatgct gcgcgccagc tccgccagtt gcacggtttc
     4261 cagcgcctgc cagacgcgtt cttcggagat atcccgcccc agcgtcacat tagcgaggaa
     4321 ggtatccgcc agcaccaccg gatcttgctg caccattgcc acgccctggc gcagcgcgct
     4381 atgacttagc gaacttaatg gacgaccatc aaggtgaatc tcaccttccg ttagcgggta
     4441 atagcccatc aataaactgg cgagggtact tttaccactg ccggtatgcc cgaccagcgc
     4501 cacaaagtta cgcgaaggca cagagagatt aatgttcttt agcaccagat tgtcatcgcg
     4561 ataagcaaat gacacgttat cgacttcgat ggtgccactc tgtaacgggt aatcatcatt
     4621 gccatattgc tggcgcggtc cgtccatcag ttcaaacacg cgctcaccag caacaacagc
     4681 ctgttgcagc atcgcctgtt gcgtggtcag ttcgattaag ggttcgttaa gtcgcccaag
     4741 atagctgata aacgcataca acacgcccac ttcaatggtg ccgctggcgg agaagccaaa
     4801 cagcatcaat aagccacaaa gaatgagcga tgaaaacaga ctcagcagcg gacgcagcag
     4861 aaaaccgtcg aggcgcaggg tttgcatcct agccatatag tgtgaacgac tggcctcccc
     4921 catacgttcg ccaaatcgcg cctgctgacg aaactgctgg ataacgctca tgccattgat
     4981 gatttcgtta aagccgtcgt tgatatccgc cagataggcg cgcacacggc ggacaatcgg
     5041 cgtgctgtaa cgctggtata tcaccattac caccagcacc accgggaaaa tcattatcgc
     5101 caccagcgcc attcgccagt cgaggctgaa catcgccacc agcatcgcgc ccaccagtgc
     5161 ggcactgcgc aggacagttg ccactacggt aacgtagaga tcgcggatca cttcagtgtc
     5221 attagtgacg cgggaaatca cctgcccgac gggttgggta tcaaactcgc ttaatggctg
     5281 gcgtaacgca gcatccatca cgtcggtacg caactgttgc actgcgccta ctgccgcccg
     5341 attaaacagc agcgactgcg cgtaatgtag cccggcggca aacagttgta gcccaacata
     5401 cgccgcagcc agccctgcaa ccactttcaa cggcaggtta tttttcgcta ccatattgtc
     5461 gataaaatag ctgataagca gcggcccact gacttctgcc gccgccgcaa cccacatcat
     5521 caggaccgca atccccagcg gtttacgcca cggcgaacca tacgctaaca ggcgcttgag
     5581 agtcggccac agttggctaa aactacgcat cgacggcctc ctcgcgaatt tccggaacgt
     5641 cgtcgagcgc cgcctccagt tgttgatagc gatacatatc gcgataccaa ccgctttgtt
     5701 gcgccagctc atcatgattg ccacgctggg cgatatgtcc gtgctgcatc acaataattt
     5761 cactggcttc cgtcagtgcc gaaaggcgat gggcactgat gattaccgtt cttccctgtc
     5821 cccactgacg caggttatgc aggatctggt gctctgtgcg tccgtccacc gccgaaagcg
     5881 catcatcaag gatgaggatt tccgcgttga ctaataacgc tcgagcaatg gagatacgct
     5941 gtttttgccc accggaaagc atcacgccgc gctcgcccac ttctgtatcg taaccttgcg
     6001 gtagacgcaa aatatcgtca tgtacgctgg ctaaccgcgc gacatgctca atctcttgct
     6061 gggtggcatt cgggcaaccc agcgcgatgt tattcgccac ggtgtcagaa aaaaggaatg
     6121 gcgtctggct gaccaccgcc aggcggctac gccagctatc gagttgtaac ttcgtcagag
     6181 gaatatcatg aaagcgaata tccccctcgc tgacgtcgaa atgacgctga atgagcgaca
     6241 acagggtact tttgccggaa ccagtcggcc cgcaaatacc cagcatctga ccgggtttca
     6301 gggcgaaatt gacgttttcc agcgcaggat ggtcagtctg cggatacgtg aactggtgaa
     6361 tatttacatc cagttcgcca cgcccttccg gcacgggttc gctaccatcg ttcaccaccg
     6421 gcgcttccgc cagcatggcg cgaatacggc tgtatgcagc actaccacgt tccacaatgt
     6481 taaacatcca tgccagcgcc agcattggcc aaatcatcag acctaaatac atcataaaac
     6541 tggtgagctg gcccagcgtt aaactgccct gcaccaccat ccaactaccg ccgccaatcg
     6601 ccagcaagtt cgccatacca atcgcgatat agatggtcgg gtcgaagcga gcatcaatac
     6661 gcgccacccg catgtttttt ttgccggtat cttcggcatc cgcggcaaat aacgccgact
     6721 ggcgatcttc cagaccaaag gctttgatca tgcggatact ggtgaggctt tcctgggtgc
     6781 ggtcattaag actggaaaac gccgcctgtg ccagcttaaa gcgttcatgc aaggcatcgc
     6841 cgttgcgctt aatcatgatc gccatcactg gcatcggtaa cagggcaaat aaggtcaact
     6901 gccagctaat ttgcgtagac atcataatca acacagcgca gcccattacc agtgaatcca
     6961 ccagcgtcag caccccttct ccggcggcaa acacgacgcg atcgacgtca ttggtcgcac
     7021 gagccatgag atcacccgtg cgatgacgca ggtaaaactc cgggtgctgc cggcttagct
     7081 gacggtaata atcttcacgc agttcaacag ccagttggta agacgcgcca aacagcaata
     7141 cgcgccagac ataacgcagg agataaacca caacggcaat cagcaccatg gtggcgatcc
     7201 acatcaggct cagcccggta gtaaagtgtt gttctgtcac gccatcgaca acaataccaa
     7261 ccacttttgg cggaaccagt tgcagcatcg cgataatgac aagcaaggcg acagccccaa
     7321 gatagcgacg ccattcccga cggaaatacc agcttaattg agcaaataat cgcacgcgtt
     7381 gtgtcctgac ctgattctgg atatttattc gatgggtaaa gaagtggtgt atttaatctg
     7441 ttccatcgcg aagctggaag tgacgtccga cagccccggc acgctgttta ccagacgctt
     7501 ataaaactcg tcgtagcgtt tcatgtcggc aacctggacg cgcatcagat aatcgtattc
     7561 accagccatg cgccagaccc ccagcacttc tggcatttcg gtaaccaccg tgacaaagcg
     7621 gcaataccat tcgctgctgt gatgttgcgt ttttatcagc acaaaggcgg tcaggccgag
     7681 gcctattttt tccggatcca gcagggcgac tttgccgata aggataccgt cgtcctccag
     7741 ccgtttcagg cgcttccagc agggggtggt tgtcagatta acggcttcag ccagtgcctg
     7801 caaagagagg gtgcaatcct gctgcagtaa ggccagcagc ttacggtcaa ttttatctaa
     7861 catagccctt ccacggagaa tttttttctc ttttaattct attttaaagg tgaaatcgcg
     7921 acaatttatt ctgtgcggtt taaacaccac ggcacaaaat gacaaattca acgcaaaaaa
     7981 aaaccgggca attgcccggt gctggaaggg atctcgttat tcgggggaat aaggtagatg
     8041 tggatagtcc agccagtgcg tcaaatagtg agagacagcc tgatttcggc aatgtccaat
     8101 caccggtaaa tgcgggagct ccgcgcgcag ttgcggcatc gcattgccca taataaaccc
     8161 gctaccgacg ctgcctaaca tttcgcgatc gttcatcgca tcaccaaagg ccatgcaatc
     8221 gcgcaacgat aaacctaaat gttgggtccg caccgtcaat gcagcgcctt tattgcagcc
     8281 caccggcagc acttcgaggc aatccgtggc ggaaaaacac aaatgtgcac gctcgcctaa
     8341 tgcttcgtat agctggatct gcaagcgtgt aagatcgtcg tgatcgccac agaagcagat
     8401 cttggtgacg ctgccgagtg gcattttttt gacatcgatt atctgataac gaaaaccgct
     8461 atagacaaat acctgcaaca acgcagggct ctctttcccg gtaaaccaac cgtcgtcatt
     8521 gaagatatgc atgctggctc gggtatccca ttgctgatac agcaccagct ccgcgacatc
     8581 cgcaggtaaa tcatcacgat gtaaaagttc accttccaga gaatgcacgc gcgttccgtt
     8641 gccggtaatc aaatatgcat ccagcgatag cgccccgaga atatgctgca tctccagcgc
     8701 atgacgcccc gtggcaaaag tgagggtaat gtcgcgttca cgcagtcgcg ccaaagtaga
     8761 gagggttttc tcacctaaat gatggtcagg catcaataaa gtgccatcca tatcaaatgc
     8821 taccagacga gccatttctt tctccactct gtgacacggt taatggtgat tgagtatcac
     8881 ctgatatata cggaagtaat agtgaatagt taaataatat tgttccgggt ttatatgcga
     8941 ttgctcaacc gtcttaacca gtatcaacgt ctgtggcaac cttcagccgc aaaccgtcac
     9001 cgtcagcgaa ctggccgaac gctgtttttg cagcgaacgc catgttcgta cgctgttgcg
     9061 tcaggcacag gaggcgggat ggctggagtg gcaggcgcag tcaggacgcg gaaagcgcgg
     9121 acaattacgc tttctggtca cgccggaatc gctacgcaat gcgatgatgg aacaggcact
     9181 ggaaaccgga aagcagcaag atgtgctgga gctggcgcaa ctggccccag gtgagctgcg
     9241 cactctgtta cagccattta tgggcggaca atggcaaaac gatacaccca cgttgcgtat
     9301 tccctactat cgcccgctcg aaccgctaca accaggcttt ttgcccggcc gtgccgagca
     9361 gcatctcgcc gggcagatat tttccggcct gacccgcttc gataataata ctcagcgccc
     9421 gattggcgat ttagcgcatc actgggaaac ctctactgac aggttacgct gggactttta
     9481 tcttcgttca accctacact ggcataacgg cgatgcagta aaagcctcac acttacacca
     9541 gcgattattg atgctgttac aactgccagc actggatcaa ttatttatta gcgtgaagcg
     9601 tattgaagtc acccatccgc agtgtctgac cttcttttta catcgccccg attactggct
     9661 tgcgcaccgg ctggcgagct attgcagcca tctggcgcat ccgcaattcc cactgatcgg
     9721 cactggtcct tttcgcttaa cacaattcac agcggagctg gtgcgcctgg agagccatga
     9781 ttattaccat ttgcgtcatc cgctgcttaa agcggttgag tactggataa ctccgccgct
     9841 tttcgaaaaa gatttgggaa ccagttgtcg gcatcccgtg caaatcacca tcggcaaacc
     9901 ggaggagctg caacgggtca gccaagtcag tagtggcatc agtttaggtt tttgctattt
     9961 aacgttgcgc aaaagtcccc gactctccct ctggcaggcg cgaaaagtga tctccattat
    10021 tcatcaatcc ggtttattac aaacgttaga agtcggagaa aacctgatca ccgccagtca
    10081 tgcattactg ccaggctgga ctattccgca ctggcaagta ccggatgaag tcaaactacc
    10141 gaaaaccttg acgctggttt atcacctacc gatagaactt cataccatgg cagaacgcct
    10201 acaggcgaca ctggcagcgg aaggctgtga actcacaatt atttttcata acgcaaaaaa
    10261 ctgggacgac acgaccctac tggcacacgc agacctcatg atgggcgaca gattaattgg
    10321 cgaagcaccg gaatatactc tggagcaatg gctacgttgc gatccactat ggccacatgt
    10381 tttcgacgct ccagcatatg cacatctgca atcgacgctg gacgcggtgc aagtaatgcc
    10441 tgatgaggaa aaccgattta atgccctgaa agcggttttt agccagttaa tggcagacgc
    10501 gacgctgacg ccgctgttca actatcacta tcgcattagt gcccctcccg gcgtgaacgg
    10561 tgtgcgactg acaccgcgcg gctggtttga atttaccgaa gcctggcttc ccgcgccgtc
    10621 gcaatgaata gctggtccgg gttaatcgca ggcgttacca taacgctttt attgttcaag
    10681 caggattatc tatgaaacgt gctgtcgttg tgttcagtgg aggccaggat tccaccacct
    10741 gtctggtgca ggcattacaa caatatgatg aagtccattg cgtgacgttc gattacggtc
    10801 agcgacatcg cgcagaaatc gacgtggcac gcgaactggc gctgaaactg ggagcacgcg
    10861 cgcataaggt tcttgatgta acgctgctca acgagctggc ggtcagcagc ctgacacgtg
    10921 acagcattcc ggtgcctgat tatgaacctg aagccgatgg catcccgaat acgtttgtcc
    10981 cagggcgtaa tattttgttc ctgacgctgg cggcaatata tgcgtatcag gtaaaagcag
    11041 aagccgtaat taccggcgtc tgtgaaacgg atttctccgg ctacccggat tgccgcgatg
    11101 agtttgtgaa agcactaaac catgccgtca gtttgggcat ggcgaaagat attcgttttg
    11161 aaacgccgct gatgtggatt gataaagcgg aaacctgggc gctggcagat tattacggca
    11221 aactggattt agtccgtaac gaaacgttga cctgctataa cggcattaaa ggcgacggtt
    11281 gcggtcattg tgcggcatgt aatttacgcg ccaacggttt gaatcattat ctggccgata
    11341 aaccgacggt gatggcagcg atgaagcaga aaaccgggtt gaggtaatta ttccgggtgt
    11401 cgccggatgc ggcttgagca tccggcacca caaaacgttt acttaaccat ctgctccagc
    11461 ttttcgcgca attccccttc cagagctaat gctttctgcg ttttaagatc aatacaaaca
    11521 aacgtaataa gcgcatccgc taccacctgc ccttccggct ccagtgtaat gacctggttt
    11581 aagatgccgc ttttaccgtt taattgctgc aactgactgg taatagttaa caggtcgctt
    11641 aataccgctg gacgacgata gttaatattg atattgacca cgacgaaggc gatgttatgg
    11701 gccgtcatcc actgaaaact gtcgctattt tccaccccat cccagcgggc ttcttcgaga
    11761 aattcaaggt agcgggcgtt gttgacgtgc tggtaaacgt cgagatgata tccacgaact
    11821 ttgatttgtg tttgcatagc gcaataacct tacgttgttg ttataagtac agaggtcata
    11881 actggtatga cctcttcagt ctggcaaaaa attgccacta tgcaaattaa ttacagggtt
    11941 aataccgcca gattacgttc caccagcgaa ttgcccatcc ccggcacctg ctttagatcc
    12001 tccaccgttt taaacggacc gtactcttcg cgataactga caatcgcctg cgctttcttc
    12061 aggccaacgc cattcatcgc gcgggctagc tcttccgcgc tggcattatt aatgctgacc
    12121 cgggtgcctt cttcgtcact ggctttcgcc ggtactgctg ctttactttg tgctgcagga
    12181 gcttccgctt tggtttctac cgccgtcggt ttcgccacag aagctgccgc cagcgcgcta
    12241 tgagacattc cggcacaggc cagggacagt gtaatgagca gtgctttaat tccgtgtttc
    12301 atactgtttt ctccttgttt gttaacagtg gaatcacctt aacgggagtg aaaacagtaa
    12361 acaaatggca atgttcagaa atggaaaagg ccgcgaaagc gaccttttct tgttacagct
    12421 gcaacgcatt tttttgcgag gcggcttcag gattattgct gttccagcgc atcgccaatt
    12481 ttgattttcg cctctttacg caggttactc atcagagctt caaagacgat ttgtgcgttg
    12541 ttctgggtga taccctgcac catcgctttt ttctgatctt ccggcattga accttgtttc
    12601 acttcatcca gcgccagcag aaccacatta ccttgcatat cggtcgccat accgtagctc
    12661 ggtttgtctt tcgctggcag tggcagtgca aacgccgcct ggctaatcgg gtcacgaccg
    12721 gaacggctta aggttttcgg ctcgccaaat ttcagaccgg cagcctgcat agcttccgca
    12781 cctttgccgg ctttcaaatc aaccagcagt ttctcagcat ccactttcgc ctgttgttca
    12841 gctttgttgt gctgaaccag tgccttaact tgttcctgaa catctgccaa cggtttcacc
    12901 gcttccggtt tgtgctcgct gacgcgcagc acgaatgcgc ggtcgccgtc tacggtgatg
    12961 atgtcagagt tgatgcccga cgcgccgttt tcacctacca gaccgccgtt aaagatagcg
    13021 tcggcaaccg gcttgaagtt caactcttcc ggcaggttat ctttgctgaa ccaacccgtc
    13081 tgagtggctt taacgccggc agcttgctct gcaccggcca gagactcggt gtcgttgctt
    13141 gccacatcgc tcactttctg ctgcagcgca tagtacgcat cgagggcttt ttcgtgtttc
    13201 actttcgccg caatgtcgtc acgtacttcg tctaacgatt tcactttcgc tggctgaatg
    13261 tcgtccagac gtacaatcag gaaaccgacc gaagatttga tgacaccaga cagttggcct
    13321 ttttctttca gaccagcatt tttcagttcg tcggggatag tggcatcttc taaccaaccc
    13381 atatcgccgc cgttacgggc agagataata tcggcagatt tttctttggc taatgcagca
    13441 aaatcaccgc ctttattcag ctcatcaagt accgctttcg cttcatcttc agttttggtc
    13501 tggatgatgc tgtagcgggt acgctgcggc tgggtgaatt gatcctgatg ctgatcgtag
    13561 tagctctgga tatccgcatc gctaaccggt tgctgcatcg ttgcggcaac cagcttgatg
    13621 taactcacgc ggaattgttc cggcgtcatg aaattgtttt tgttttgttc gtagtagctg
    13681 gcaatctcct gttcggtcac aggctgcttc gccgccagcg cgttaacatc gatagtcgcc
    13741 tcacgcacca cgcgttgttg cgcgaccagt gccgccagct cgtcggtttc acctttcagc
    13801 ataaaatcgg taccggcaac gccgttaatc agctgttggg tagtgagctg gttacgcagc
    13861 gcctgggcgt actgatcggc ggtcatcccc atctggttga ggataccgtt atagcggctg
    13921 ttatcaaatt tgccgtcaac ctggaaggct ggggtcgcga aaatcgcctg tttaacctgc
    13981 tcatcactga tacccagttt cagctcacga gcgtactgat ccaacagcgc ctcgtcgatc
    14041 agacgattca gcacctgttg acgcagggtt ttcatatagc cttcgttcgc tgccagctcg
    14101 gagtattgat cgcccagctg ttgctgcata cgattacgct cgctgttaaa ggcgttctcg
    14161 aactgcccac ggctgatttc ctggtcattc acttttgcgg cgtagttatt gcctccgcca
    14221 atcaggtaac cactcacgcc ggtcaatatg aacgacacga taatgatacc gaaaataatc
    14281 ttgagcacga gactgtttgc agccgtgcgt aagctgtcca tcatggtgta acaacactcc
    14341 gctgtagatg actgtgtacc tcacgcaacg tatcgatgcg cgcaaaggcc tattgtgaca
    14401 agaaacgggg gcaattgtca gcccacaaga cacagaaagt caccgaatac aaataaaaaa
    14461 ggcacatcag tagatgcgcc cttgaacttc gtcacatccc cactggggac aacgcttagt
    14521 ttaccgcgtc tttcagtgct ttacctgcac ggaagctcgg tactttagcg gcagcgatgg
    14581 tgatctcttt accggtctgc gggttgcggc cagtacgggc agcacgctct ttaacggcaa
    14641 aagtaccaaa acctaccagt gctacatcat ccccttcttt cagagattca gttacggaag
    14701 caataatagc atctaacgca cggccagccg cagctttaga gatatcagcc cctgcagcaa
    14761 tcttgtcgat caattgagat ttattcactc ttctcttcct ctttataatt tatatcgcac
    14821 ttgaatcctt caaggtacga acgcgcagca gttatatcag gccagccatc cccttacaac
    14881 accccttaat aagggcaagc ccgatccgct atctaactta gcgagacaaa aaaaggctgg
    14941 caagcccgaa ttagcctgcc agccctgttt ttattagtgc attttgcgcg aggtcactat
    15001 tttgcagtca caacctgcat gccagacggt tcattttgca gcgccagagt cagaacttcc
    15061 tcaatgcgct tcacaggatg aatgtccaga tcggcaatta cgttgtcagg aatctcttcc
    15121 agatcgcgtt tattttcgaa cggaattagc actgttttaa tcccgccgcg atgcgctgcc
    15181 aggagttttt ctttcaaacc accgatcggc agtacctgac cacgcagagt gatctcaccg
    15241 gtcattgcca catcggcacg aaccgggtta ccggtcaggc aagaaaccag cgcggtgcac
    15301 atagcaatac cggcactcgg accatctttc ggcgtcgcac cttccggtac gtggacgtgg
    15361 atgtcacgtt tttcgtaaaa atcagggttg atccccagtt tttccgcacg cgcacgaacc
    15421 accgttaacg ccgcctgaat agactcctgc atcacttcgc cgagcgaacc ggtataggtc
    15481 agtttgcctt tacccggaac acatgcggtt tcaatggtca gcaagtcacc gcccacttcc
    15541 gtccacgcca gaccgattac ctgaccgaca cggttttcgt tatcagcgcg accatagtcg
    15601 aaacgctgaa caccgaggta gtcatgcagg ttatcgccgt taatttcgat atgttttaat
    15661 gacttatcga gcagtaactg cttaaccgct ttgcggcaca gtttggagat ttcacgctcc
    15721 agaccacgca cgcccgcctc acgggtgtag taacgaataa tgccgataat ggcgctatcg
    15781 tcgacggtca gctcaccttt tttcagtgca ttacgttcaa tctgcttcgg cagcaggtga
    15841 cgtttggcga tgttcagttt ttcatcttcg gtataaccgg agaggcgaat cacttccata
    15901 cgatccagca gcggtgccgg aatgttcatg gagttcgacg tcgcgacaaa catcacgtcg
    15961 ctgagatcgt aatccacttc caggtagtgg tcgctgaacg ctacgttctg ctctggatcc
    16021 agcacttcaa gcagtgcaga ggccggatcg cctcgcatgt cagaagacat tttgtcgatc
    16081 tcatcgagca ggaacagcgg atttttcacg cccactttcg ccattttctg gatcagttta
    16141 cccggcatag aaccgatgta agtacggcgg tgaccacgga tttccgcttc atcacgcacg
    16201 ccgcccagcg ccatacggac atatttacgc ccggtggctt tggcaatgga ctgaccaaga
    16261 gaggttttac ctacccccgg cggccctacc aggcagagga tcggtccctt gattttgttg
    16321 acacggcttt gaaccgcaag gtactcaagg atgcgatctt tcacgcgctc cagaccataa
    16381 tggtcggtat caaggatttc ctgcgcctga cgcaggtctt ttttgacctt gctgcgcgca
    16441 ttccacggta cctgtaccat ccagtcgata taaccacgca ctacggtcgc ttctgccgac
    16501 atcggagaca tcattttcag cttctgcaac tctgcttccg ctttctcttt tgcctctttc
    16561 ggcatcttcg ccgcgtcgat tttgcgcttc agggcttcgt tttcgtccgg cacgtcgtcc
    16621 atttcaccga gttctttctg aatagctttc atttgctcgt tcagatagta ctcacgctgg
    16681 gatttctcca tctgcttttt aacgcggttg cgaatgcgtt tctcaacctg cagcagatcg
    16741 atttccgatt ccatcattgc catcagatat tccagacgtt cgttaacgtc ggacatctcc
    16801 agaacggact gtttgtcagc cagtttcagc ggcatatgtg cagcaatggt atccgccaga
    16861 cgcgccggat cgtcgatgct attcagcgac gtcagcactt ctggtgggat ttttttgttc
    16921 agcttgatgt agccttcgaa ctggctgatt gcagtacgca ccagcacttc ctgttcccgc
    16981 tcatcaatgg tcggcgactc cagatactcc gccttcgcag aaaagtgttc gccattgtca
    17041 gagagcgcag aaatacgcgc gcgctgtaac ccctcgacca gcactttgac ggtgccgtca
    17101 ggcagtttca gcatctgcaa tatagaggcc acggtcccga cggtgaaaag atcgtttaca
    17161 cccggctcat ccgttgaagc ttctttctgc gcgaccagca taattttttt atcatggtcc
    17221 atcgccgctt ccagacaacg gatagatttt tcccgcccga caaataaggg gatgaccatg
    17281 tgcggataaa ccaccacatc gcgcagcggc aatacgggga tttcaatgcg ttcagaacgc
    17341 tcaggattca tagagctctc tcttagttta atttccgcca ggtaatcaga tgacacgact
    17401 gtgcttcacg ccatctatta acatgtacgt cagtatatgg ggatgtttcc cccacattca
    17461 acgccgagaa tagaggaaaa attaaagggg agataaaatc cccccttttt ggttaactaa
    17521 ttgtatggga atggttaatt attcaccaga tgcctgttgc gcttccggct taccataaat
    17581 cagcaacggt ttgctttgac catcaattac cgactcgtcg ataaccactt tttcgacgtc
    17641 ttccatggac ggcagatcgt acatggtatc gagcagtgcg gcttctacga tggaacgcag
    17701 gccacgggca ccggttttac gcgccatcgc tttcttagcg atagcatcca gcgcctcgtc
    17761 acggaattcc agatccacgc cttccagatt aaacagcgcc tgatactgct tggtcagggc
    17821 gtttttcggc tctttgagga tctgaatcag agcttcttcg ctcagttcat tcaacgttgc
    17881 tacaaccggc agacgaccaa tgaactcagg gataagacca aacttgatca gatcttccgg
    17941 ttcaacctgc gccagcagct cgccttcgct tgctttgtcg gacttcgctt ttaccgtcgc
    18001 gccaaaacca atgccggagc cggtttctac acggtgggaa atcactttat ccagaccggc
    18061 aaacgcaccg ccacaaataa acaggatctt agaggtatca acctgcaaga attcctgctg
    18121 cggatgttta cgcccacctt gcggtggaac agcagctacc gtaccttcga tcagtttcaa
    18181 cagtgcctgc tgtactcctt caccggaaac gtctcgggta atggacgggt tgtctgactt
    18241 acgagaaatc ttgtcgattt catcgatgta gacaatcccg cgctgcgctt tctgtacgtc
    18301 gtaatcgcac ttttgcaaca gcttctgaat gatgttttca acgtcttcgc ccacataacc
    18361 ggcttcggtc agcgtggttg cgtcggccat ggtgaacggg acgtccagca ggcgcgccag
    18421 cgtttcagcc agcagcgttt taccggaacc ggtcggaccg atcagcagaa tgttactttt
    18481 gcccaactcg acaccattgc tggtatcgcc gttgcgcaga cgtttgtaat ggttgtatac
    18541 cgcgaccgcc agcacttttt tcgcctgttc ctggccgata acgtaatcgt ccaggtggtt
    18601 gcgaatttca tgcggcgtcg gtagcgcact gcgttcacga tgcggtgcaa cttctttaat
    18661 ctcttcgcga atgatgtcgt tacataaatc aacacattcg tcgcagatat acacggatgg
    18721 accggcaatc agcttgcgca cttcatgctg gcttttgccg caaaaagagc aatacagcaa
    18781 tttgcctgag ccatctttgc gtttatctgt catgggtcaa aacctcttct ttgttctttg
    18841 tgccgcacac gacgacgcaa atgccattct caggcgcaag ccgcttacag cgttgtgccg
    18901 ccctagataa gtatagcggc acagttgcgc ctctggcatc aattacgatg ggtcagaatc
    18961 gaatcgacca gaccgtattc caccgcttca ggggcggaaa ggaagcgatc gcgctcggta
    19021 tcacgttcaa tctgttctaa tgattgaccc gtatgaagcg ccataagttc attcatgcgc
    19081 cctttaactt tcagaatttc acgggcatga atttcgatat cggtcgcctg gccctggtag
    19141 ccgcccaacg gttggtgaat catcacgcgc gaattcggca ggcaaaaacg tttacctttt
    19201 gcccctgcgg tcaacaagaa agcgcccatc gaggccgcct ggcccataca gatggtgctg
    19261 acatcaggct tgataaactg catggtgtca tagatagaca tcccggcagt gatcaccccg
    19321 cctggggagt taatgtacag atagatatct ttttctgggt tttccgcttc caggaacagc
    19381 atctgcgcca caatcaggtt agccatgtgg tcttcaacct ggccagtcag aaaaatgacg
    19441 cgttccttaa gtagacgaga atagatatca aaagagcgct caccgcgtga ggtctgttca
    19501 atgaccatcg gcaccagcgc catatggggt gcaaagttat ctcgttcgcc gctgtatgac
    19561 atttccgtct cctggataaa attgaaaaaa acctgctgta ccgattgtaa ccttatggac
    19621 ggattatcag ctagtccctg tgttatgggt acggcatcac catatttcaa gcataacaat
    19681 cttttgttgt tacgctaaca ccgaaagagg cttttcgcac ggctccatgc aaaattcatg
    19741 acaaaaaaaa gcccaccacc tggcggtgac gggcctttgt gcgaatttag cgcgttatgc
    19801 tgcgtaaatt acgcctgctg gttcatcagc tcgttgaaag tggtttcttt ttcagtcact
    19861 ttcgctttcg ccagtacagc ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg
    19921 tccatcagtt ctttgttttt gctgtagaac tcgataactt ctttcggatc ttcgtacgca
    19981 gaagccatct cttcgatcag gcctttcacg cgctcttcgt cagctttcag ctcgttggtg
    20041 cggataactt cgcccagcag caggccaaca actacgcggc gtttagcctg ttcttcgaac
    20101 agttcgcgcg gcagttccag agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg
    20161 cgacgcagaa cgtcgatttc gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct
    20221 tttaccagac cttcgatcgc ctgagactta acgcggttac ggatggcgct cttcagctcg
    20281 cgctccatgt ttttacgcac ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg
    20341 aaacgtttga tgaattctgc agtcagttcc ggcagttcac gctcttcaac tttcttcagg
    20401 ttgatagcga atttcgctgc tttacctttc aggttttctg cgtggtattc ttccgggaag
    20461 gtcacgtcga tggtgaactc ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc
    20521 gggatcatac gaccctggcc catcgccagt acgaaatcag acgctttacc gccttcgaac
    20581 tcttcgccgt ctacagaacc ggtgaagtcg atggttacgc ggtcttctgc ttcaacagcg
    20641 ccgtcttttt ctttccaggt cgcctgctgt ttacgcagag tatccagcat gccgtcaacg
    20701 tcagcgtcgg tcacttcaac gatcggtttt tcaacttcga tcgcttccag accctgcagt
    20761 tcaacttccg gataaacttc aaactctaca gagtaagtga agtcttcacc cagcttgtat
    20821 tcgcccggaa cataagtcgg tgcgccagcc ggattgattt tttctttaat gatggcgtca
    20881 atgaagttac ggctcatcag gtcacccaga acgtcctggc gtacagacgc gccataacgc
    20941 tgagcaacga tattcattgg cactttgcct ttgcggaagc cgtcaatacg tacttttttc
    21001 gcaacgttga ccagctcgct tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc
    21061 gttacacggc ggccaaggcc ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa
    21121 aaaatcacag tgctcggtca actcttaccc cgcaagcaca ggttgttggc ttcgcggaac
    21181 cgggatgttc ttataatcaa tcacattccc tgttaccaga atcatcccga aagcattcaa
    21241 ataggacgcg gcattatagc ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg
    21301 tcgcgtattt tttcacaatt cccgggcaat tttcgtctga atgctgggca aaaagcgcaa
    21361 aatccagaca aaacaaaacg gcccgcaggc cgtttttcat aaaatcttta gcaacatact
    21421 ggaaaagcgc cgacagttgc aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg
    21481 aggagaggca aagacggtgt cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag
    21541 agccagcgca tgaacggtag tagagagttc ctccgctaaa gtactgtaaa tcattcgatg
    21601 acgattcaga aaacgttcac ccgtaaaacg atcgctgacc agcacaactt taaaatggct
    21661 ttcagagccg gctgggacat tgtgacgata gctttcatcc actacttcga ggaatacggg
    21721 ttggaacgcc gcccttaatt tttcttctat ccgctcacgt atcatcatga aatatctcct
    21781 ccgacaacgc tgagatgtca tccatccctt taaatactag ccgcttttac cgtttccatt
    21841 gcagcttaac aacaaatatt taggtttcgt cctggttttt ccgtcaagcg tatgaagttt
    21901 actgatttaa agattgaaac acaggcctcg catcagcgtt tcttttacca ggggtgatta
    21961 tttcgtcaac atgatgaatt tacatgcctt acccacttcc cctcgccgtt ggcaatgtta
    22021 tgatggcggg aatttttacc aaacacgctt aagagtctct gagagcccga acatgttcaa
    22081 aaaaatcctc ttcccgttag ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac
    22141 aactattgaa gtttccccga cgattacact gccacagcag gatccaagcc tgatgggcgt
    22201 caccgtaagc attaatggtg ccgatcagcg taccgatcag gcgctggcaa aagtcacccg
    22261 cgataatcaa atcgttaccc tgaccgcctc ccgcgctctg cgtttcctgc tgcaagaagt
    22321 gctggaaaaa cagatgaccg cgcgtggtta catggttggt ccgaatggcc cggttaatct
    22381 gcaaatcatc gttagccaac tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat
    22441 cgctaccaaa gcggatatcg ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa
    22501 aaactatcgt gccagctaca acgttgaagg tgcgttccag gcctccaaca aaaatatcgc
    22561 cgatgcggtt aacagtgtgc tgaccgatac catcgctgat atgtctcagg acaccagcat
    22621 tcacgaattc atcaagcaga acgcgcgtta atttctgccc tctggcccgg tgcaagccgg
    22681 gcctgtagac gcccatgtcc agtcaatatt tacgtatttt tcaacagccg cgttcagcca
    22741 tattgctgat cctgggtttt gcttccgggc taccgctcgc cctgacatcc ggcaccttac
    22801 aggcctggat gacggtcgaa aatatcgatc tcaaaaccat tggtttcttc tctctggtag
    22861 gccaggctta cgtttttaaa ttcctctggt caccgctgat ggaccgctac acgcccccat
    22921 tttttgggcg gcggcgcggt tggctgctcg ccacgcaaat cctgttatta gtcgccattg
    22981 cggcgatggg ttttctcgaa ccaggcaccc aactccgctg gatggcggcg ctggcggtgg
    23041 tgatcgcttt ttgctctgcc tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc
    23101 tcccggcaga agaacgtggt gcgggcgcgg caatcagcgt gctgggttac cgtttaggga
    23161 tgctggtttc cggcggccta gccctgtggc tggcagataa atggttgggc tggcagggca
    23221 tgtactggtt gatggcggcg ctgttgatcc cctgtattat cgcgacgttg cttgcaccag
    23281 aaccaaccga caccattcct gtgcccaaaa cgctggaaca agcggttgtt gcacctctgc
    23341 gagatttctt tggtcgcaat aatgcctggc ttattttgct tcttatcgtg ctgtataagc
    23401 tgggcgacgc attcgccatg agcctgacaa ccacgttttt gattcgcggc gtcgggtttg
    23461 atgcgggtga agtaggcgtg gtaaacaaaa cgcttggctt actggcgacc attgttggcg
    23521 cattgtacgg tgggattttg atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg
    23581 gcattttaca aggtgcgtct aacgctggtt actggctgct gtcgattact gataagcatc
    23641 tctacagcat gggcgcagcc gtctttttcg aaaacctctg tggcgggatg ggcacatcag
    23701 cctttgtcgc gctgttaatg acgctgtgta ataagtcatt ttccgctact caatttgccc
    23761 tgctctcagc gctttccgct gtagggcgag tttatgtcgg ccccgtggcg ggttggtttg
    23821 ttgaagcaca cggctggtcg acattctatc tattctccgt cgccgctgcc gtaccggggc
    23881 ttattttgct gctggtttgc cgccagacgc ttgaatatac acgagtaaat gacaacttta
    23941 tctcccgtac cgaatatccg gcaggttatg cctttgccat gtggacactg gcggcgggcg
    24001 tcagcctgtt ggccgtgtgg ttacttctgt tgacgatgga cgcgctggat ttgacgcact
    24061 tctctttcct gcctgctctg ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc
    24121 ttggtggttt gctggattat ctggcgctac gaaaaacgca tctgacgtaa tctgtaaata
    24181 ttatttagct gtagtaatca ctcgccgtaa ttattacggc taaataaaca tcagcatcgc
    24241 ttattaatat ttgttctttt gtgcggctta gcgtttggaa attattgacg ccatttataa
    24301 attctatttt tcttacacga ttcagctaat gagtctttat tttctcacca cccagttgtc
    24361 actttaatga taattatttg ttaaataatt gttttatttc gcattggtta taccaattgc
    24421 ccaccaagac tccttctgca ctcccctttt gtta
//
LOCUS       DAZOZX010000052        24089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZOZX010000052 DAZOZX010000000
VERSION     DAZOZX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.052"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUPT_002056"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUPT_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0819636.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUPT_002057"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUPT_002057"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0819637.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACHUPT_002058"
     CDS             1531..2478
                     /locus_tag="ACHUPT_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR0819638.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACHUPT_002059"
     CDS             2548..3354
                     /locus_tag="ACHUPT_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0819639.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACHUPT_002060"
     CDS             3364..3771
                     /locus_tag="ACHUPT_002060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0819640.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="ACHUPT_002061"
     CDS             3771..4265
                     /locus_tag="ACHUPT_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR0819641.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="ACHUPT_002062"
     CDS             4331..5128
                     /locus_tag="ACHUPT_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0819642.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="ACHUPT_002063"
     CDS             5139..5927
                     /locus_tag="ACHUPT_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR0819643.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUPT_002064"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUPT_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR0819644.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUPT_002065"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUPT_002065"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR0819645.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="ACHUPT_002066"
     CDS             complement(8370..10001)
                     /locus_tag="ACHUPT_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0819646.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUPT_002067"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUPT_002067"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0819647.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUPT_002068"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUPT_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0819648.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="ACHUPT_002069"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="ACHUPT_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="ACHUPT_002070"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="ACHUPT_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="ACHUPT_002071"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="ACHUPT_002071"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR0819649.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="ACHUPT_002072"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="ACHUPT_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="ACHUPT_002073"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="ACHUPT_002073"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0819650.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="ACHUPT_002074"
     CDS             23609..24052
                     /locus_tag="ACHUPT_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0819651.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5944 a         6146 c         6108 g         5891 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAZOZX010000053        23855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZOZX010000053 DAZOZX010000000
VERSION     DAZOZX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.053"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            133..636
                     /locus_tag="ACHUPT_002075"
     CDS             133..636
                     /locus_tag="ACHUPT_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0819652.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(716..967)
                     /gene="yecJ"
                     /locus_tag="ACHUPT_002076"
     CDS             complement(716..967)
                     /gene="yecJ"
                     /locus_tag="ACHUPT_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0819653.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1082..1168)
                     /gene="azuC"
                     /locus_tag="ACHUPT_002077"
     CDS             complement(1082..1168)
                     /gene="azuC"
                     /locus_tag="ACHUPT_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0819654.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1431..1754
                     /locus_tag="ACHUPT_002078"
     CDS             1431..1754
                     /locus_tag="ACHUPT_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0819655.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1925..2422
                     /gene="ftnA"
                     /locus_tag="ACHUPT_002079"
     CDS             1925..2422
                     /gene="ftnA"
                     /locus_tag="ACHUPT_002079"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0819656.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2459..2698)
                     /locus_tag="ACHUPT_002080"
     CDS             complement(2459..2698)
                     /locus_tag="ACHUPT_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0819657.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2888..4099
                     /gene="tyrP"
                     /locus_tag="ACHUPT_002081"
     CDS             2888..4099
                     /gene="tyrP"
                     /locus_tag="ACHUPT_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0819658.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4161..4826)
                     /gene="yecA"
                     /locus_tag="ACHUPT_002082"
     CDS             complement(4161..4826)
                     /gene="yecA"
                     /locus_tag="ACHUPT_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0819659.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5022..5108)
                     /locus_tag="ACHUPT_002083"
     tRNA            complement(5022..5108)
                     /locus_tag="ACHUPT_002083"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5072..5074),aa:Leu,seq:taa)
     gene            complement(5121..5194)
                     /locus_tag="ACHUPT_002084"
     tRNA            complement(5121..5194)
                     /locus_tag="ACHUPT_002084"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5160..5162),aa:Cys,seq:gca)
     gene            complement(5249..5324)
                     /locus_tag="ACHUPT_002085"
     tRNA            complement(5249..5324)
                     /locus_tag="ACHUPT_002085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5289..5291),aa:Gly,seq:gcc)
     gene            complement(5476..6024)
                     /gene="pgsA"
                     /locus_tag="ACHUPT_002086"
     CDS             complement(5476..6024)
                     /gene="pgsA"
                     /locus_tag="ACHUPT_002086"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0819660.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6081..7913)
                     /gene="uvrC"
                     /locus_tag="ACHUPT_002087"
     CDS             complement(6081..7913)
                     /gene="uvrC"
                     /locus_tag="ACHUPT_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0819661.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7910..8566)
                     /gene="uvrY"
                     /locus_tag="ACHUPT_002088"
     CDS             complement(7910..8566)
                     /gene="uvrY"
                     /locus_tag="ACHUPT_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0819662.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8708..8779
                     /locus_tag="ACHUPT_002089"
                     /pseudo
     CDS             8708..8779
                     /locus_tag="ACHUPT_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8862..9038
                     /gene="yecU"
                     /locus_tag="ACHUPT_002090"
     CDS             8862..9038
                     /gene="yecU"
                     /locus_tag="ACHUPT_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0819663.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            9025..9249
                     /gene="yecF"
                     /locus_tag="ACHUPT_002091"
     CDS             9025..9249
                     /gene="yecF"
                     /locus_tag="ACHUPT_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0819664.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9282..9902)
                     /gene="sdiA"
                     /locus_tag="ACHUPT_002092"
                     /pseudo
     CDS             complement(9282..9902)
                     /gene="sdiA"
                     /locus_tag="ACHUPT_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(10132..10884)
                     /gene="tcyN"
                     /locus_tag="ACHUPT_002093"
     CDS             complement(10132..10884)
                     /gene="tcyN"
                     /locus_tag="ACHUPT_002093"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0819665.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(10881..11549)
                     /gene="tcyL"
                     /locus_tag="ACHUPT_002094"
     CDS             complement(10881..11549)
                     /gene="tcyL"
                     /locus_tag="ACHUPT_002094"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0819666.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11564..12550)
                     /gene="dcyD"
                     /locus_tag="ACHUPT_002095"
     CDS             complement(11564..12550)
                     /gene="dcyD"
                     /locus_tag="ACHUPT_002095"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0819667.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12655..13455)
                     /gene="tcyJ"
                     /locus_tag="ACHUPT_002096"
     CDS             complement(12655..13455)
                     /gene="tcyJ"
                     /locus_tag="ACHUPT_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0819668.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13543..14094)
                     /gene="fliZ"
                     /locus_tag="ACHUPT_002097"
     CDS             complement(13543..14094)
                     /gene="fliZ"
                     /locus_tag="ACHUPT_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR0819669.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14140..14859)
                     /gene="fliA"
                     /locus_tag="ACHUPT_002098"
                     /pseudo
     CDS             complement(14140..14859)
                     /gene="fliA"
                     /locus_tag="ACHUPT_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(15024..16676)
                     /gene="fliC"
                     /locus_tag="ACHUPT_002099"
     CDS             complement(15024..16676)
                     /gene="fliC"
                     /locus_tag="ACHUPT_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR0819670.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            16942..18348
                     /gene="fliD"
                     /locus_tag="ACHUPT_002100"
     CDS             16942..18348
                     /gene="fliD"
                     /locus_tag="ACHUPT_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0819671.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            18373..18783
                     /gene="fliS"
                     /locus_tag="ACHUPT_002101"
     CDS             18373..18783
                     /gene="fliS"
                     /locus_tag="ACHUPT_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0819672.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            18783..19148
                     /gene="fliT"
                     /locus_tag="ACHUPT_002102"
     CDS             18783..19148
                     /gene="fliT"
                     /locus_tag="ACHUPT_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0819673.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19226..20713
                     /gene="amyA"
                     /locus_tag="ACHUPT_002103"
     CDS             19226..20713
                     /gene="amyA"
                     /locus_tag="ACHUPT_002103"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0819674.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(20747..21160)
                     /gene="yedD"
                     /locus_tag="ACHUPT_002104"
     CDS             complement(20747..21160)
                     /gene="yedD"
                     /locus_tag="ACHUPT_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0819675.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21347..22552
                     /gene="yedE"
                     /locus_tag="ACHUPT_002105"
     CDS             21347..22552
                     /gene="yedE"
                     /locus_tag="ACHUPT_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR0819676.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            22549..22782
                     /gene="yedF"
                     /locus_tag="ACHUPT_002106"
     CDS             22549..22782
                     /gene="yedF"
                     /locus_tag="ACHUPT_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0819677.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            22889..23559
                     /locus_tag="ACHUPT_002107"
                     /pseudo
     CDS             22889..23559
                     /locus_tag="ACHUPT_002107"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(23599..>23855)
                     /locus_tag="ACHUPT_002108"
     CDS             complement(23599..>23855)
                     /locus_tag="ACHUPT_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0819678.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         5889 a         6124 c         5736 g         6106 t
ORIGIN      
        1 tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag aaaggaatta
       61 ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact tctggatata
      121 aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca aatgaaccgc
      181 gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga acagagtctg
      241 aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca aatgatgcgc
      301 atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat tgatgttcca
      361 ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga atacgagcaa
      421 cgttcaagca ctctggcgca gttagccgat gaagcgaaag aactgaatga tgattcaacc
      481 gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggcctgtt gctgcaaacc
      541 attcttgatg aagtgcgcag tgcgaaactt gcgggtatgt gccctgtgca gaccgaccaa
      601 catgttctga atgtcgtgtc acaccagctg cattgatcat acatcggcgc taatacattg
      661 cgccgatgaa gtttttgaga aaccgctgcc tcatctgttt gaagcagcgg tttttttaat
      721 gggattcacc ctgtggggta aacttgagtt caatcagcgc gatggctttt tggattgccc
      781 gcatggtgac cgggtctgcg gcagcgggat gatgagtaaa gtcgatattc ttcagctgac
      841 tggacatttt ttcacgaact tcaacgggcg cgattacatc gagaacatcc agaatttgtt
      901 tgataaccaa ctggcaagct accacatcag aaaccagttc ctgatcggca ttcagcggct
      961 gggacatcgt aaactcctga tagcattttg aaagccgtta tagtagcggc ttcacatctt
     1021 cagcgatagt cacatccacc gtcatcagga cacaaaaaaa cctgccggag caggtttttt
     1081 gttatcggaa catattgcct ggcggtacgt ctttgaacgt cttgcaatag ttattgaaca
     1141 tacttttcag gattttgcgc agtttcatcg cggcactccg accatttgtt atacaggtgt
     1201 tattgtcttt gcgcttataa tatgacaacc atcacaaaaa tcaatcttta tgtgatacaa
     1261 atcacataaa taccccttta atgttataaa aatgataatc aaaaaacagc ccccctattt
     1321 ctgacaccta cagatggcaa gaaatagcgc ctgccaggcg tcttttccgg ccattgtcgc
     1381 agcgctgtaa cgcgtaaaat agtgctttct cttactcttc tggctggacc atgagacttc
     1441 tgattctgac tctttcatta ataacgctgg cggggtgtac ggttactcgc caggcccatg
     1501 tgagcgaagt tgacgcggca acaggtattg tacggttggt ttatgaccag gcttttttgc
     1561 agcatgccca taccgatcgt tatgtcagtc gcgggatcgc cgatcgcgca tgccagcagg
     1621 aaggctatac ccacgcgatc ccctttggtc agccagtagg caactgtagc ctttttgcgg
     1681 gttccttatg tttgaatacg gaattcactt tgtcatatca gtgccatcat tccgccttcc
     1741 ctgtctttct ctaaatattt taatcagcga ggggatcttc gctgattaaa gaaatagcgg
     1801 taatgcgttt tattcccatt cgtattttta ataattgaag tttatatttt accttttgca
     1861 aataataaaa taacaaatta tagtgacgcc acagcaacaa atataacctt tgtggagcac
     1921 tatcatgctg aaaccagaaa tgattgaaaa acttaatgag cagatgaacc tggaactgta
     1981 ctcttcactg ctttatcagc aaatgagcgc ctggtgcagc tatcatacct tcgaaggtgc
     2041 tgccgcgttc ctgcgccgtc acgcccagga agagatgacg catatgcagc gtctgtttga
     2101 ttacctgact gataccggca atttaccgcg tattaatacc gttgaatctc cgtttgctga
     2161 atattcctca cttgatgaat tattccagga aacctataaa cacgaacaat taatcaccca
     2221 gaaaattaac gaactggctc atgctgcaat gaccaatcag gactacccaa catttaattt
     2281 cctgcagtgg tatgtttctg agcagcatga agaagagaaa ctgttcaaat cgattattga
     2341 taaattaagc ctggcaggca aaagcggcga aggtctgtat tttatcgaca aagaactctc
     2401 taccctcgac acacaaaact aatgctctcc ggcggcaggt ttctgccgcc gtttcaaatc
     2461 agtgacggca aatcttactc tgatcggtcg aaaaaccttc ttctgaaggt ataaatttgc
     2521 cttttgctgc cagaaacgct accagctctc ccgctgtcac cccttctgcc gagcacgtgt
     2581 gaaaacgtgc ctgttcacca aaacgcgctt taatcgcagc ttccagactg gcatgcgtat
     2641 attgctcgcc tgattcaatc atcatattta acacttcatg accgtgaata gagtccatcg
     2701 ttcctcctca aaaaaagcct agcgtagcga ttaccgctta tgaagacttt gcgccagcgc
     2761 aggactgaat gcttttattg tacatttata tttacacaat atgtaacgtc ggtttgacga
     2821 agcagccgtt atgccttaac ctgcgccgca gatatcactc ataaagatcg tcaggacaga
     2881 agaaagcgtg aaaaacagaa ccctgggaag tgtttttatc gtggcgggaa ccacaattgg
     2941 cgcaggcatg ctggcaatgc cgctggctgc ggccggtgtt ggttttagcg ttacgttaat
     3001 cttgttgatt gggctttggg cgttgatgtg ctacacggcg ctattactgc tggaggtgta
     3061 ccagcatgtt ccggcagata ccggtctggg cacgctggca aaacgctatc tgggacgcta
     3121 cggtcaatgg ctgacgggct tcagtatgat gttcttaatg tatgctctga ctgcagcata
     3181 catcagcggt gccggtgaat tgttggcctc cagcatcagc gactggacag gtatttcaat
     3241 gtcggcaacc gctggcgtgc tgttgttcac ttttgttgcc ggtggcgtgg tttgtgttgg
     3301 aacatcgctg gtcgatttat ttaaccgttt tctgttcagc gccaaaatta tttttctggt
     3361 ggtgatgctg gtattgctgc taccgcatat tcacaaagtg aatcttttaa ccctgccgtt
     3421 gcaacagggg ctggcactgt ctgcaatccc ggtgattttt acgtcgtttg gttttcacgg
     3481 tagcgtgccg agtattgtca gctatatgga tggcaacatt cgtaagctac gctgggtgtt
     3541 tataatcggt agtgccatcc ccctggtggc atatattttc tggcaggtgg cgacgcttgg
     3601 cagcattgat tcaacaacct ttatgggatt gctggctaat catgctggat taaacgggct
     3661 gttacaggcg ttacgcgaaa tggtggcctc tccgcatgtt gagctggcgg tgcatttatt
     3721 tgctgattta gccctcgcca cgtcatttct cggcgttgcg ttaggcttat ttgattatct
     3781 ggctgatttg tttcagcgtt caaataccgt tggtggacga ttgcaaactg gtgcaattac
     3841 ctttctgccg ccgttggcgt ttgcactgtt ttatccacga ggatttgtga tggcgctggg
     3901 ttacgccggt gtggcgctgg cggtactggc attgattatc ccttcactat tgacctggca
     3961 aagcagaaag cacaatcctc aggcgggtta ccgggtcaaa ggtggtcgtc cggcgctggt
     4021 ggtggtgttt ctctgtggta ttgctgtgat tggcgtgcaa tttttgattg cggcagggtt
     4081 gttaccagaa gtggggtgat cagatagcct caaattcctt attgggtgcc agaattaacg
     4141 ctgacaccca atgtgacctc ttaatgcagg cagcactgct taaatttctt accactgccg
     4201 cacggacaag gatcgttacg ccccggtttc tcttctgctt tgatcggttg ctgaacagct
     4261 ttttcctgcg gatgcgccat ccagtatgca tgtaaatcaa gcgccgccag tcgaatggca
     4321 tctacgctct cttcaaacgc ttctggcgac attttctcta cccgctcgaa gttttcctca
     4381 gtaccgtgca gcgcaatcgc ctccagcgct ggttttaaag aatcgggcaa cgttgaccag
     4441 tcagaaagtg ccacgccccg catatagcca aagcaccact cctcaacaat cgtcagctcg
     4501 ctgccatcaa cttctcgcaa gccgaataac ggctcaaact gctccgggaa ttcgttcaga
     4561 cgctctgcgg tatcggccat atgttgaaaa gccaggttca taaagcgcgt catctctttc
     4621 tctgacgccc aacgcggcac atagtcagcc ccaccccaca cggcaaccag ccactgttct
     4681 ggttcaatct cttgcggaga actcaacacc gccgtcaata aaccgtccag ctccgccaca
     4741 tcaaggatgg cgtggtcagt gttgtatttg gtcagaatat cgtccagcca ttccaactca
     4801 ctttcgttta acggtcccgt tttcatacgc ttttccttgt ggatctcaac tcgccagcac
     4861 ctatcttaca tgccggtccg tatcagagat actttttgag tggctttgcc ggtgattaaa
     4921 aattaaggag ggtgtaacga caagttgcag gcacaaaaaa accacccgaa ggtggtttca
     4981 cgacactgct tattactttg attttattct tatctttccc atggtacccg gagcgggact
     5041 tgaacccgca cagcgcgaac gccgagggat tttaaatccc ttgtgtctac cgattccacc
     5101 atccgggctc gggaagaaag tggaggcgcg ttccggagtc gaaccggact agacggattt
     5161 gcaatccgct acataaccgc tttgttaacg cgccaaattc ttcaggcctt tcagccagac
     5221 atccgcttga cgccgatgtc ttttaaactg gagcgggaaa cgagactcga actcgagacc
     5281 tcgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcattcat caagcaatca
     5341 gttaatcact tgattttatt atcgtctggc aatcagtgcc gccgttcgat gcgttgcatt
     5401 ctacttacct ggcgcgatga gtcaacgata tttttcacca cttttgatcg tttgctgaaa
     5461 attacgccga aacgatcact gatcaagcaa atctgcacgc gcagcgctca aatattgcaa
     5521 cattgaccac agagtcagta ccgcagccac aaagaaaagt gcaataccga cgtactcaac
     5581 ccaaatgttc ggacgccaca gcagccaggc caacgccacc atctgggcag tggttttcac
     5641 tttcccaatc caggagacgg ccacgctact gcgtttaccc aactccgcca tccattcgcg
     5701 tagcgcagaa ataataattt cacgggcgat catcgttgcc gccggtaatg tcacccacca
     5761 gctgtgataa tgctcggtta ccagcaccat ggcgatagcc acgagaactt tatctgccac
     5821 agggtcaagg aaagcaccaa accgggtact ctggttccag cggcgtgcca gaaaaccatc
     5881 gaaccagtca gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg gcgaccaggt
     5941 gacaggcaga taaaagacca atacaaagaa tgggataagg atgacacgga acagtgtaag
     6001 caacgtaggg atattaaatt gcataatgac gggtaactat ctgttgtcag taagattacc
     6061 cctatgttgc tacagagaca tcaatgtttc aacgaccaga agatcttttc tgccagacct
     6121 tgcgaaatac ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa accttgcaaa
     6181 ccgcccatat atttcaacaa catttgccga cgttttggcc cgacgccttc aatggtttcc
     6241 agggaactgg tatttttgac cttcgcccgt tttttacggt gcccgccaat cgcgtgatca
     6301 tgtgattcat cgcgaatatg ctggataaca tgcagcgcgg gtgaatctgg cggcaaacta
     6361 aatccctcac cttccggctc aaagaacagc gtttccagtc cagccttacg atctgctcct
     6421 ttggcaacgc caagtagcag cggatgattt ttatcccatg agacatccag ttcggcgaag
     6481 acatttttcg cctgcgcaag ctggcctttg ccgccgtcga taaggatcac atccgggatc
     6541 ttactgtcgt caatggcttt accataacgc cgacgcagca cctgattcat cgccgcataa
     6601 tcatcgcccg gcgtgatgcc tgtaatgtta tagcgccgat actccgcacg cagcgggccg
     6661 ttagcatcaa acaccacaca ggaagcgacg gtttgttcgc ccatggtatg gctgatgtca
     6721 aagcactcca tccgcttcac ttccggcaat ttcaacacgc tggcaagcgc ggtcagtcgc
     6781 tggtgaacgg tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc attggtgcgc
     6841 gcgagtttca gataacgcgc cctatcgccg cgaggtttgg tttgaacatt aatcttgcgt
     6901 cccgccagtt ctgaaaggga atcggcgagc agcgttttat cgctaagatt aaaatcgagc
     6961 aggatctcac ccggtaaggt gcgcatctgg ctgccttgta aatagaactg acctacgaag
     7021 gtttctacca cctcgctcag ttccgtaccg ccaggcactt tcgggaaata gctgcggctg
     7081 ccgagcactt tgccctgacg aatgaacaat acgtggacac aagccatgcc cgcatcgaac
     7141 gccacaccaa taacgtcaag gtcgtcgccg gtattggaaa caaactgttt ttcggtgacg
     7201 cgtcgcaccg cctgaatttg gtcgcgaata catgccgctt cttcaaactc cagattctgg
     7261 ctggcagttt ccatacggct aatgagttgc gtaagcacct gatcatcttt gccagacaaa
     7321 aacaggcgca catactcgac ctgctgagcg tattcttctt cactcaccag tccttcaacg
     7381 cacggtccca gacagcgccc tatctggtat tgcagacacg gacgcgagcg attgcgataa
     7441 acactatttt cgcactggcg aatggggaaa atcttttgca gtagcgccag tgtttcacgt
     7501 acggcatagc cattcgggaa cgggccgaaa tattcacctt tggcatgctt tgcaccacga
     7561 tgcatcgcca gacgcgggtg ggtatcgcca ctcaggaaga taaaaggata agatttatca
     7621 tcgcgtagca aaacgttgta acgcggctga tagagtttga tgtagttgtg ttccagcaac
     7681 agcgcttcgg tttctgtatg cgtaaccgtt acatcaattt gctggatctg ggcgaccagc
     7741 gcttcggttt tgcgcgaagc gaggttgctg cggaaatagc tggaaagccg ttttttcagg
     7801 tctttcgctt tgccgacata gataaccgta ccaccagcat cgtacatgcg ataaacgcct
     7861 ggctggctgg ttacggtttt taaaaacgct tttgcgtcaa actgatcact cactgacttg
     7921 ataatgtctc cgcattacac agaccatggc gaattgccag gtgagtcagc tcaacatcgc
     7981 catgaatgtt tagtttactg aacatacgat agcggtagct gttcaccgtt ttcggactga
     8041 gattgagctg ttctgagatc tcattgacct tctggccctt agtgatcatc agcataatct
     8101 gcaattcacg ttcagacaaa ctggcaaatg ggctttctgt tttttctggt tcgatctggc
     8161 ttaacgccat ttgttgagcg atatcagaag caatgtaacg ctgccctgaa tagacagaac
     8221 gaatcgcact cacgacttcc tgcggagccg cgcctttgct gaggtagcca gcagcacctg
     8281 cctgcatgac tttcgctggt aaagggtttt ctgtatggac cgtaagcatg atgattttaa
     8341 catcagctgt ggaacgcgcg attttacgcg tcgcctcaag accgccaatg cccggcatac
     8401 tcatgtccat tagcaccacg tcaacggcat ttgtccggca ccacttaacg gcgtcttcac
     8461 cgcacgatgc ctctccgacg acttttatac ccttaatatc ttccagaatg cgtcgtatcc
     8521 ctgcgcgcac cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga atatctccag
     8581 aaatagggat aacgctactg atagttagtc attcgtatat tagcggtttt tattgcaact
     8641 ttgaaacgtt aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa ttcgattgtc
     8701 cacagttatg gtcacgcccg tcaccaacgg cttaccggat cgtttcacag taagccctac
     8761 gatgaaatgt gacaaaaatt gactttattc agcaaaaatg aaaatcagcc cgctgaatat
     8821 gtaacattaa ttaacccgaa caatacgggc aaaaaacatt catgataaaa atatttattg
     8881 gtcattatat taacgtattt tatagcactg ccgatatcac gctcaaaaaa caaccactgc
     8941 tatttttagc aaagcttatg gtatactccg ccgccttaac atttttcacc acaaattttc
     9001 attgcaacat gacgaggaaa ataaatgagt acgcctgatt tttctactgc cgagaataat
     9061 caagaactgg caaatgaagt ctcctgcctg aaagcgatgc tgacgctgat gctgcaggcg
     9121 atgggacaag ctgacgcggg ccgcgtgatg ttaaagatgg aaaaacagct tgcgctgatc
     9181 gaagacgaaa cccaggctgc agtattttcc aaaacggtta agcaaattaa acaagcctac
     9241 cgtcagtaat ataaaaccgg ctgagagcgt gcctttcagc cggttattga caaaatcatc
     9301 gctatctctg ctgatgtctt cccttccgcc gtccatttca gaatttcttt ttcgcgcttg
     9361 ctgaaattca tctctggcgt catcactatt tcatcattta aacgcatcag agccatcaga
     9421 ctttcgcgca ccagtaactg catttttaat tgcagttcat cactaagaat gggtatttcg
     9481 cgcgtgctgc aacgggaaaa ggacaaaaag cccagcgccc gatttggcag cattaaatac
     9541 tgagtgacac cgcggcgtaa accatgtgcg tgcgcggctt cccataatgg ctgtgcttcg
     9601 ctgaataagt catcattcca cattaaatgg ccctgactaa agttttcagg gttcagcaca
     9661 ggatcaattg cgagaaaatt ttttgcctga taataactaa cccacgactc agggtaattg
     9721 gtgtagaaag ccactttagg tcgcgtgaat ggtaccgggt ggcgaacaca taacgaatag
     9781 taatcgtact ccagctgctg agcctgaagc tctatttcat ggtaggcctc ttctgcggcc
     9841 tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc tgaaaaaatc cgtatcctgc
     9901 atagtaaacc gcaacgcccc tgagagtgag tatcatttat aatgataatt caaactatca
     9961 cataaataag atttatatat aatttatatt attcaggcaa tgaattactt ttgcaagcca
    10021 tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagttt tatcacgctt
    10081 gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt attattgcag
    10141 cagaagcttc tcgaggaact ggcgggtgcg aggctgctgg ggatcggcaa ataacgcttt
    10201 tgctgccccc tgctcgacta tccgcccctg gtccataaag atcgcccggt ccgcaacatc
    10261 ccgggcaaag ctcatttcgt gtgtcacaat caccatcgtg cgcttttcct gcgccagctg
    10321 gcggatggtg ttcaggactt cgcccaccag ctctggatcc agcgccgacg ttggctcgtc
    10381 aaacaaaatc acctcaggac gcattgccag tgcacgcgca atcgcaacac gctgctgttg
    10441 accgccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa ctttcgccag
    10501 cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga tcaccggccc
    10561 ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct ggaagacaaa
    10621 cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc tgacttaatg aacgtgcagt
    10681 atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc ggttgttcca gcagatttat
    10741 gctacgtagc aatgtggttt tgccggaacc actcggaccg ataattgcca ccacttcgcc
    10801 aggctttacc tcgaggtcga taccgtgcag caccgtctga ccgtggaatt ttttcaccag
    10861 gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag ttgattctca
    10921 aaatggttct gcaacgtcga taacacagtc gccatgatcc agtagatgag cgaagccgcc
    10981 aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg acggaacagc
    11041 tccggcacct ggattgtcgc ggccagcgac gtatctttta ccagactgat aaagctgttc
    11101 gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg catggtctgc
    11161 cacggcgtca taccaatact ggcagcggct tcccactgac ctttatcaat agaagaaatg
    11221 gcggcgcgca gcgtttcggc ggcataggcg gcagtgttca gcgacagacc aatcattgct
    11281 gacggaatgg gatctaattc gataccaaac tgcggcaggc cgtaatagat cataaacagt
    11341 tgtgcgatga gcggcgtacc acgaaaaata gagatataaa aacgcgccag ccagcgcacg
    11401 ggccagattg gcgacaggcg catcagcgcg agaataaacc cgagtagtaa gccaaaaaac
    11461 atgccgccga tactgagttg cagggtatac ccagcccctt tcaacaggaa cggcaaggaa
    11521 tcaataacca gttgtatact ttcttgcatg agcgttcccg gagctaaacg tggggatgat
    11581 aggcgaacag cgcaggcgcg ccgccggtat gaataaacag aatcggccct tcatctttga
    11641 agcgtttctg actgataccg tcaatcagcc ccgccatcgc ttttccggta tacacaggat
    11701 caagcagaat gccttccaga cgcgccagca atttcactgc ttccatgcct tcgtcgttcg
    11761 gcacgccgta gccaggtgca aaatagtcat cccagagtaa aatttccgct gatgcggtca
    11821 gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaat tgatcggcaa
    11881 cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt tccagcccaa
    11941 cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac taccaccgac gaaatattaa
    12001 ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataacct agcgcgccaa
    12061 gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct tcgactcgcg
    12121 tcgccagttc ttccagttgg gcattgggat cggtcagtgc gtcgcacatt tcaatctggg
    12181 tattgaacag atccagcaac aaacgattgc cgttggttaa atagttttct gcggttgtgc
    12241 caataggatt ttccagcagc gccacgcagt gcagaccgag tttcgccgcg actgcggcag
    12301 tctggcgcac atggttagac tggatcgcgc cggcagtaat cagcgtatcg gcaccttcgc
    12361 gcagggcatc tgccgcgaga aattccagct tacgtaattt attgccgccc attgccatgg
    12421 gggtgacctc atcccgtttg atgaaaattt cccgtccaag ataatcagaa aagcgcggca
    12481 gatattcgag cggcgttggc gcgccgataa actccaaccg tggaaaacgg gttaaattat
    12541 gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt tcttattatg cacgctgaaa
    12601 acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt tgtcattatg ctgattattt
    12661 ggtcacatca gcaccaaacc atttttcgga aagagcttgc agagtgccgt ctttttgcat
    12721 ttccgcaatt gcatcattca ccgctttcag caggtcttca tttcctttac gcagcgccac
    12781 gccagactcc tgacgggaga atgcttcacc ggttactgcc agcgtatcgt tggttttctt
    12841 caccagatcc agcgccgcca gacgatcaac gaggatcgca tcgatacgcc ctacgcgcag
    12901 atcctgatat ttggtcgggt catcatcata ggtacgcaca tcgacaccct gaacattctg
    12961 ccgcagccac tcttcatagt tggtgcccag accgacaccc acttttttgc ctttcagatc
    13021 agcagccgtt ttaatggtgc cttcgttacc ttttttcacc agcgcctgaa taccggaaat
    13081 ggtgtaaggg gtagagaaat cgtatttttt cttgcgctcg tcagaaatgg tgacctgatt
    13141 aatcaccaca tcaatgcgtt tagagtccag cgacgccagc ataccgtccc atttggtcgg
    13201 ttttagtgac gcctcaacgc caagatgttt tgccagctgt tgggcaaatt ccacttcaaa
    13261 accggttaat ttgccgtcat ccccctgaaa actgaacggc ggataagtgc cttccagccc
    13321 taccagcagc gtgccgctct ctttgacttt attaagcaga ccttcatctg caaaactttt
    13381 aacgctcatg cccgcaacca gcgccacggc catcacaccc atcaatgcct gacgtcccag
    13441 atgtgctaat ttcatattca ccccgaatgt tgttatgtct gtttgcagtg tagagccatt
    13501 gtttgtaaac acaaaaacaa ctccgctaca tcttattctt atttaatata tatcagaagc
    13561 aggcaggctg gaggatttct gcacaagtcc ggtacaggtt tggcgctgat agtgttgata
    13621 cttccgtaat gcgatgcggt aattgttggt gctggtggtg ggcagccacg gggcgaggat
    13681 ttcatcaagg aaaccgtcct gcagctgatc gagggaaata ttttgctcat gtagatgatt
    13741 acccaaacgg cgcagacgaa cgacatattc acgcaccgta cctgggctca tttcagtttg
    13801 ctcaaaaaga aatttcttaa agccgataat atcgaaaaaa tcactctgcg ttttgcaatg
    13861 taaatcaccg caaaatcggc acaatgccgc ccatgattgt tgttccgttt gccagccttt
    13921 ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta ttcactattt tgccgtcgcg
    13981 aactaaggta atgcgatcga gtaatttacg gcaatgcgcg caatgggtct ggctgtgttt
    14041 aaagtcttta agatagcggc ttaatggccg tcttttcagg tgctgcacca tcattaagaa
    14101 ctcctggtag tcaaagttaa agtgcggcat ttactgacgt tataacttac ccagtttagt
    14161 gcgtaaccgt ttaatgtcct ggctgtgtaa ctgactgacc cgcgattccc cgacctccag
    14221 caccgcgcca atctctttga gattcagctc ttcctggtaa tagagggtta ataccagttt
    14281 ttcgcgctcc ggcaacgttt cgatggcttc catcacccgc tggcgcagat tactgtccag
    14341 cagttgttgt agcgggtttt ctcgctgatg atcatcagta accagttcga tgctatcgcc
    14401 gtgctcttca cgccactcat cgtaggagaa gagctggctg ttattggtgt cgagcagcat
    14461 ttgacgataa tcggcaatat cgatccctaa acgttccgct acctcagttt ccgtggcgtt
    14521 gcggccaagt tcctgctcca gttgccctat tgcctgtgcc acttcacgcg cgttgcgtcg
    14581 cacgctgcgc ggcacccagt cacggctgcg aagttcatcc agcatagcgc cacggatacg
    14641 ctacactgcg taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt cgacagcatt
    14701 aagtaaccca atgccgcccg cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg
    14761 aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc acagcgagtg
    14821 tttatccatt acaccttcag cggtatagag tgaattcacg ataaacagcc ctgcgttata
    14881 tgagttatcg gcatgattat ccgtttctgc agggttttta atcggacgat tagtgggtga
    14941 aatgaggggt tatttggggg ttacgcaaaa agccgaatgt aaaaaacccc gccggtggcg
    15001 gggtttgaga ggcattcaga cgattaaccc tgcagcagag acagaacctg ctgcggtacc
    15061 tggttggctt ttgccagcac ggagttaccg gcctgctgga taatctgcgc tttcgacatg
    15121 ttggacactt cggtcgcata gtcggcgtcc tgaatacggg actgcgcttc agacaggttg
    15181 gtagtggtgt tgttcaggtt ggtgactgcg gaatccaaac ggttttgcac cgcacccagg
    15241 gaggagcgga atttgtcgat ggagctgatg gcttcatcca gagctttcag gggatcggct
    15301 gtcgtagctg cttttgtttc agcatctgtc gtcaatttac catcagcatc tttataaact
    15361 gccttaccgc tgccgttagt cactgaacca tcatcctgta ggtaaaaatc tacagtatca
    15421 gatgtggtca acgcctcgtt gttatcaaca taaaccgaat caacaccttt tgttacagcg
    15481 ccagttgtgc cgttgacgct ataacctgca gtagtgaaat caacattgtt tcccgaggtt
    15541 gatacagctt tatcgaaagc tgcctgatca acttttgcac ctgttactga ataagtaata
    15601 gtgtccggcg tagtactatt accggtaaag gagatagagg taccgccaat gtcaatggtt
    15661 gtcgctttgg tattattggc tgcaagtgtg gacagactag cctcagtcaa agaagcacca
    15721 gagccgtttt tcgttaagcg cccagttgta tcaatgtaga gtttatctcc attgcttgcc
    15781 gtaattttac catcactgct gatctgaaca tcctgtgcag aaccatcgat agtaatagtg
    15841 cccttagcgg tatcaccaac tcccggggtc aaatatttct gaacatcggc agctgaagcc
    15901 gttgtggtat caaaagagta tgacttactt gcactgtcat acttgtaatt cgttgcacta
    15961 gccgccgttc cgaagccgtt attaatggtt gcctgaacag tatcaccatc actaactcca
    16021 gccagcaaat cagacgcttt agcagcatcg taacccgcac tcacagtata tttgttgccg
    16081 accactgttg cattagcagc taccaagtca gctttagatg cagcagtatt agccacagcc
    16141 ccgccaccat tcacattaaa cccattcagc cccagcgtat cagagtcaat tttcttcaga
    16201 tcaatagtga tagtctggcc gtcattcgca ccaacctgaa ttttcatcga accgtctttt
    16261 gccagtacgt tcacgccgtt gaactgggtc tgaccggata cgcggtcaat ttcgtcgaga
    16321 cgggatttga tttcgtcctg aatggagtcc agatccgaat cagagttagt cccggtagaa
    16381 gcctgaaccg tcagctcacg gatacgctgt aagttgttgt tgatttcgga cagcgcgcct
    16441 tcagtggtct gtgcaacaga aataccgtcg ttggcgttac gtgcagcctg agtcaggcct
    16501 ttaatgttag aagtaaaacg gttagcaatc gcctgacccg cggcgtcatc cttcgcgctg
    16561 ttaatacgca agccagaaga cagacgctcg atagaactcg acagcgcaga ctggttcttg
    16621 ttgatattat tttgagtgat cagcgagagg ctgttggtat taatgacttg tgccatgatt
    16681 cgttatccta tattgcaagt cgttgattac gttttgggtt tccacccgtc ggctcaatcg
    16741 ccgtcaaccc tgttatcgtc tgtcgtaaaa caacctttag aatttttttc acaaacagcc
    16801 attttttgtt agtcgacgaa atactctttt ctctgcccct tattcccgct attaaaaaaa
    16861 acaattaaac gtaaactttg cgcaattcag gccgataacc ccggtattcg ttttacgtgt
    16921 cgaaagataa aaggaaatcg catggcaagt atttcatcgc tgggagtcgg gtcaggtctg
    16981 gatttaagtt caatccttga tagcctcacc gccgcgcaaa aagcgacgct aacccctatt
    17041 tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct acggtacgct gaaaagcgcg
    17101 ctgacgactt tccagaccgc caatactgca ttgtctaaag ccgatctttt ttccgccacc
    17161 agcaccacca gcagcaccac cgcgttcagt gccaccaccg cgggtaacgc catcgccggg
    17221 aaatacacca tcagcgtcac ccatctggcg caggcgcaaa ccctgaccac gcgcaccacc
    17281 agagatgata cgaaaacggc gatcgccacc agcgacagca aactcaccat tcaacaaggc
    17341 gacgacaaag atccgattac cattgatatc agcgcggcta actcatcgtt aagcgggatc
    17401 cgtgatgcca tcaacaacgc aaaagcgggc gtaagtgcga gcatcattaa cgtgggtaac
    17461 ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc ttgataatgc gatgacactc
    17521 tcggtcagcg gtgatgatgc gctacaaagt tttatgggct atgacgccag tgccagcagc
    17581 aacggtatgg aggtctcggt cgccgcccag aatgcgcagc tgacggtcaa caacgtcgcc
    17641 atcgagaaca gcagcaacac tatcagcgac gcgctggaaa acatcactct gaacctgaac
    17701 gatgtcacca cgggcaacca gacgctaacc atcactcagg acacctccaa agtgcaaacg
    17761 gcgattaaag actgggtgaa tgcctataac tcgctaatag ataccttcag cagcctgacc
    17821 aaatacaccg ccgtagatgc gggagctgat agccagagtt ctagcaatgg cgcactgctc
    17881 ggcgactcca cgctgcggac gattcagacg cagttgaagt cgatgctgag taataccgtc
    17941 agttcttcca gctataaaac gctggcgcag attggtatca cgaccgatcc cagcgatggc
    18001 aaactggaac tggatgccga caaactcacc gctgcactga aaaaagatgc cagcggcgta
    18061 ggtgcattga ttgttggcga tggtaaaaaa accggcatca caaccaccat cggcagcaac
    18121 ctgaccagtt ggctttcgac aacgggcatt attaaagccg ctaccgatgg cgttagtaaa
    18181 accctgaata agttaactaa agactacaac gccgccagcg atcgcattga tgcgcaggtc
    18241 gcgcgctaca aagaacaatt tacccaactg gacgttttaa tgacctcgtt aaacagcacc
    18301 agcagctact taacgcagca gttcgaaaac aacagtaatt ccaagtaatc attattcatc
    18361 gggagacagg tcatgtacgc ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa
    18421 agcaccgtaa tgagcgccag ccaacagcag ttggtcacca tgctatttga tggagtgctg
    18481 agcgcactgg ttagagcgcg actgtttatg caggacaaca atcagcaagg caaaggcgtc
    18541 tctttgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct tgatgaagag
    18601 agcaaagacg aattaaccca aaacttaatt gctctttata gctatatggt caggcgtttg
    18661 ctgcaagcca atttacgcaa cgatgtctca gcagtcgaag aggtggaagc attaatgcgt
    18721 aatattgccg atgcctggaa agagtcttta ctctcccctt cttcgattca ggacccagtc
    18781 tgatgaacaa tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc
    18841 tcatgttacg cctggcaacg gaagaacaat gggacgaact catcgctagc gaaatggcgt
    18901 atgtgaatgc ggtgcaggag atcgcacacc tgactgaaga ggttgccccg tccactacga
    18961 tgcaggagca gctccgcccg atgctgcacc tgattctcga caacgaaagc aaggtaaagc
    19021 agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa
    19081 aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg caggataacc
    19141 tcttttgaat cttaatgagc cgatggctcg cgaataatcc gattacggct acgcttctaa
    19201 tattcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg
    19261 gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgcag acggttttaa
    19321 tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcggcta
    19381 ttcggtcggc tacgactcct atgatttatt tgatctaggt gagtttgatc agaaaggcag
    19441 catccctact aaatatggtg ataaagcaca actgctggcc gccattgacg ccctgaaacg
    19501 taatgacatt gcggtgctgc tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa
    19561 agaagctatt cgcgtgcagc gtgtaaatgc tgatgaccgt acgcaaattg acgaagaaat
    19621 cattgagtgt gaaggctgga cgcgttacac cttccccgct cgtgccgggc aatactcgca
    19681 gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaacacc ctgatgaaga
    19741 tggcattttt aaaattgtta acgactatac cggagaaggc tggaacgatc aggttgatga
    19801 tgaactcggt aatttcgatt atctgatggg tgagaatatc gattttcgca atcatgccgt
    19861 gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggctt
    19921 tcgtcttgat gcggtcaaac atattccggc atggttttat aaagagtgga tcgaacacgt
    19981 acaggaagtt gcgccaaagc cgctgtttat tgtggctgaa tgctggtcgc atgaagtcga
    20041 taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgtttg atgcgccgct
    20101 gcagatgaaa ttccatgaag catcgcgcat gggtcgcgac tacgacatga cgcagatctt
    20161 cactgggacg ctggtggaag ccgatccttt tcacgccgta accctcgttg ccaaccacga
    20221 cacccaaccg ttgcaagccc tcgaagcacc ggtcgaaccg tggtttaaac cgctggcgta
    20281 tgccttaatt ttgttgcggg aaaatggcgt tccttcggtg ttctatccgg acctctacgg
    20341 tgcacattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata tgccaataat
    20401 cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacatt
    20461 atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg aatatcccgg
    20521 ctgcgtggtg gtcatgtcga acggagatga tggcgaaaaa accattcatc tgggagagaa
    20581 ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg tagtgacaga
    20641 cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga
    20701 agaggtgatt taaatccatc cccggcggca agccggggag atttcattac ggcagtggcg
    20761 tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct
    20821 ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac
    20881 gttttacggt aacgttagtg agatcatcag acatcaatgt caacttaccc ggcacggcaa
    20941 tcccccgctg ccactgacgg caatccaagg tatcgccctc tttagtgaca atcaggctgc
    21001 ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag tagcccgcca
    21061 gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg
    21121 ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct gcatgagaag
    21181 aaaagaggat tgtggcatta aagctctgat gtcgccagcc tttccaggtt cagagagaaa
    21241 tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc atggtgtatg
    21301 attcgcggct cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca
    21361 attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca tcgcggcggg
    21421 tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca cgggtgaatt
    21481 tacccgttgg ggcggtcagc tcctgcagct gtttggcgtc catgctgaag agtggggtta
    21541 ttttaaaatt atccatctgg aaggatcgcc attaacccgg atcgacggca tgatgatcct
    21601 cggtatgttt ggcggctgct ttgctgctgc gctgtgggcc aacaatgtca aactacgcat
    21661 gccacgcagc cgtatccgca ttatgcaggc catcattggc ggcattatcg ccggttttgg
    21721 cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggcattc ctcagttctc
    21781 gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcgcgctt
    21841 taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc
    21901 gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt
    21961 cggcatgctg ggctgggcgc tgctcacggc gatgaaccaa ccaaaactgg ggctggcaat
    22021 gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct ttacttcggc
    22081 gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca ttatcggtat
    22141 ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac ctaaaatcat
    22201 gtgggcggga ccaaatgcgg taattggtgg tttacttttt ggttttggca tcgtgctggc
    22261 aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg
    22321 ggtcggtctg ggcaacgtga tcggctcaac gattctggcg tattactggg atgatttcgc
    22381 tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacttttg gtccgatggg
    22441 aggcctgctg gtgacatatt tgctgctgtt tgctgcgctg atgttgatta tcggctggga
    22501 aaaacgcttc ttccgccgtg aggcaccgca gactgctaag gagatcgcat gaaaaatatc
    22561 gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc agtcgcaacc
    22621 cttgaggcga tgccgcaatt aaaaaaaggt gagatcctgg aagtggtgag cgactgtccg
    22681 cagtcgatca ataatattcc actggatgcg cgtaatcacg gctacacggt gctggatatt
    22741 cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg
    22801 gcccacagcg ggaacatcgt tacctctgac acatagattt tacccgcccc ctctcctatt
    22861 cttttcagta tgatctcagg aggttgctat gtgtggacgc tttgcccaat cccaaacgcg
    22921 tgaggattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct acgatcccga
    22981 acccattggc agatacaatg tcgcgccggg aaccaaagtt cttctgctca gtgaacgtga
    23041 tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt gggataaacc
    23101 gccgctgatt aacgcccgcg tagaaactgc ctcaaccagt cgcatgttta aaccgctctg
    23161 gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa aagaaggcga
    23221 caaaaaaagc cttattttat ctatcgcgct gacggacaac ctgtttttat ggccgcgata
    23281 ggtagcatac cgtttgagcg tggtgacgaa gccgaaggat ttttgatcgt cactgcggcg
    23341 gcagatcaag gtctggtaga tattcatgac cgccgcccgc tggttctgtc gccagaagct
    23401 gcacgggaat ggatgcggca agacattggt ggtaaagaag cctcagaaat cgctaccaga
    23461 agctgtgtgc cagcaaacca gttcacctgg caccctgtgt cgcgcacggt gggtaatgtc
    23521 aaaaaccagg gcgcggagtt aattcaacct gtttgttgac atcctccacg ccctgaatga
    23581 cgaggacccc tgctacgttc aggctgtcgc ctgaatcatt tcggtgggtt cctgcttcaa
    23641 cgagcggcct gactgcacca tctctccaca ggcaagaacg gcgtgccccg ccgctaaaat
    23701 gttacgagct ccatttacat cggcgttcgc tgtatatcca catacctggc atctgaattt
    23761 actttgtgac aggcgatttt ctttcgctgt atgaccacag cacgcgcaac gctggcttgt
    23821 gtacgctggc ggaacagcaa gcacctgacc gccac
//
LOCUS       DAZOZX010000054        23414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZOZX010000054 DAZOZX010000000
VERSION     DAZOZX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.054"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..247
                     /locus_tag="ACHUPT_002109"
     CDS             <1..247
                     /locus_tag="ACHUPT_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0819679.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="ACHUPT_002110"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="ACHUPT_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0819680.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="ACHUPT_002111"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="ACHUPT_002111"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0819681.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="ACHUPT_002112"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="ACHUPT_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR0819682.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /gene="ybjH"
                     /locus_tag="ACHUPT_002113"
     CDS             complement(3258..3542)
                     /gene="ybjH"
                     /locus_tag="ACHUPT_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0819683.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="ACHUPT_002114"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="ACHUPT_002114"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0819684.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="ACHUPT_002115"
     CDS             complement(4443..5651)
                     /locus_tag="ACHUPT_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0819685.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="ACHUPT_002116"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="ACHUPT_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0819686.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="ACHUPT_002117"
     CDS             complement(6446..8131)
                     /locus_tag="ACHUPT_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0819687.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="ACHUPT_002118"
     CDS             8401..8778
                     /locus_tag="ACHUPT_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0819688.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="ACHUPT_002119"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="ACHUPT_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0819689.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="ACHUPT_002120"
     CDS             9225..9512
                     /locus_tag="ACHUPT_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0819690.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="ACHUPT_002121"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="ACHUPT_002121"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0819691.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="ACHUPT_002122"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="ACHUPT_002122"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0819692.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="ACHUPT_002123"
     CDS             11269..11745
                     /locus_tag="ACHUPT_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0819693.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="ACHUPT_002124"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="ACHUPT_002124"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0819694.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14470
                     /gene="potG"
                     /locus_tag="ACHUPT_002125"
     CDS             13304..14470
                     /gene="potG"
                     /locus_tag="ACHUPT_002125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0819695.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLR
                     PINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQ
                     LSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVT
                     HDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQE
                     DGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHI
                     AYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14480..15433
                     /gene="potH"
                     /locus_tag="ACHUPT_002126"
     CDS             14480..15433
                     /gene="potH"
                     /locus_tag="ACHUPT_002126"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0819696.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15430..16275
                     /gene="potI"
                     /locus_tag="ACHUPT_002127"
     CDS             15430..16275
                     /gene="potI"
                     /locus_tag="ACHUPT_002127"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0819697.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16335..16823
                     /locus_tag="ACHUPT_002128"
     CDS             16335..16823
                     /locus_tag="ACHUPT_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0819698.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16864..17991
                     /gene="rlmC"
                     /locus_tag="ACHUPT_002129"
     CDS             16864..17991
                     /gene="rlmC"
                     /locus_tag="ACHUPT_002129"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0819699.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18166..18897)
                     /gene="artJ"
                     /locus_tag="ACHUPT_002130"
     CDS             complement(18166..18897)
                     /gene="artJ"
                     /locus_tag="ACHUPT_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0819700.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19189..19857)
                     /gene="artM"
                     /locus_tag="ACHUPT_002131"
     CDS             complement(19189..19857)
                     /gene="artM"
                     /locus_tag="ACHUPT_002131"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0819701.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19857..20573)
                     /gene="artQ"
                     /locus_tag="ACHUPT_002132"
     CDS             complement(19857..20573)
                     /gene="artQ"
                     /locus_tag="ACHUPT_002132"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0819702.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20580..21311)
                     /gene="artI"
                     /locus_tag="ACHUPT_002133"
     CDS             complement(20580..21311)
                     /gene="artI"
                     /locus_tag="ACHUPT_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0819703.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21329..22057)
                     /gene="artP"
                     /locus_tag="ACHUPT_002134"
     CDS             complement(21329..22057)
                     /gene="artP"
                     /locus_tag="ACHUPT_002134"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0819704.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22275..22790)
                     /locus_tag="ACHUPT_002135"
     CDS             complement(22275..22790)
                     /locus_tag="ACHUPT_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0819705.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         5759 a         5764 c         6045 g         5846 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccctaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatgctaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaactgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg cgccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta tttactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgccagcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgg gcgcatccgg
    13501 ctgtggcaag tccacgctgc tgcgtatgct ggcaggtttc gaacaacctt ctgccggaca
    13561 gataatgctt gatggcgtcg atttgtcaca ggttccgcct tacctgcgcc ccatcaatat
    13621 gatgtttcag tcttacgcgc tgtttccgca tatgaccgtg gaacagaaca tcgcttttgg
    13681 cctgaaacag gacaaactac cgaaagcgga aatttccagt cgggtcaatg agatgctcgg
    13741 cctggtgcac atgcaggagt tcgccaaacg taaaccgcat cagctttctg gtggtcagcg
    13801 acagcgtgtg gccctggccc gaagccttgc caagcgcccg aaactattac tgctcgacga
    13861 gccgatgggc gcgctggata aaaagctgcg tgaccggatg cagcttgaag tggtggatat
    13921 tctggagcgc gtcggtgtga cttgtgtgat ggtcacccac gatcaggaag aggcgatgac
    13981 catggcgggg cgcatcgcca ttatgaatcg tgggaaattt gtccagattg gcgaaccgga
    14041 agagatctac gagcatccga ctacccgcta tagcgccgaa tttattggct cggtaaacgt
    14101 ctttgaaggc gtactcaaag agcgtcagga agatggcttg gtgcttgatt cgccggggct
    14161 ggtgcatcca ctgaaagtcg acgcggatgc ctcggtggtc gataacgtgc cggtacatgt
    14221 ggcgctgcgc ccggaaaaaa tcatgctttg cgaagagccg cccgccaatg gttgtaactt
    14281 cgcggtgggg gaggtgatac acattgccta tctcggcgat ctttcggtgt atcacgttcg
    14341 tctgaaaagt gggcagatga ttagtgccca gctacaaaac gcccatcgtc atcgtaaagg
    14401 gttaccgacc tggggcgacg aagtgcgttt gtgctgggaa gtggacagct gtgtggtgct
    14461 gacggtttaa ggagcaaaga tgaatacact tgaacctgct gcccagtcga aaccgccggg
    14521 cggatttaag ctgtggttgt cgcagctgca aatgaagcat gggcgcaaac tggtcattgc
    14581 gttgccatat atctggttga tcttgctgtt tctgctgcca tttctgattg tctttaaaat
    14641 aagcctggcg gagatggcgc gcgctattcc accttatacc gaactgatgg agtgggctga
    14701 cgggcaactg tccatcactc ttaatctcgg taattttctg caactgaccg acgatccgct
    14761 ctatttcgat gcttatctcc agtcgctaca ggtggctgcg atttcgacat tttgctgttt
    14821 actgatcggc tatccgctgg cgtgggcggt ggcgcacagt aagccttcga cccgtaatat
    14881 tttattacta ctggtgatcc tgccgtcgtg gacctcgttt ctgatccgcg tttatgcttg
    14941 gatgggaata ttaaaaaaca acggtgtgct gaataatttt ctgctgtggc tgggggttat
    15001 cgatcaaccg ctgaccattc ttcataccaa tctggcagtt tatattggca ttgtttacgc
    15061 ctacgtgccg tttatggtgc tgccgattta taccgcgttg attcgtattg attattcgct
    15121 ggtggaagca gcgctggatc tcggtgcacg accgctgaaa acgttcttta ctgtgatcgt
    15181 gccgctgact aaaggtggga ttattgccgg atcgatgctg gtgtttatcc cggctgtggg
    15241 cgagtttgtg atcccggaac tgctcggtgg cccggacagc atcatgatcg ggcgcgtgct
    15301 atggcaagag tttttcaata accgcgactg gccggtggcc tcggcggtag cgatcatcat
    15361 gttgctgctg ctaattgtgc cgataatgtg gttccacaaa caccagcaaa aaagcgtggg
    15421 agaacacgga tgaataattt accggtagtt cgttcgccct ggcggattgt gattttgctg
    15481 ctgggcttta cctttctcta cgcgccaatg ctgatgctgg ttatctattc gtttaacagc
    15541 tcgaagctgg tgacggtgtg gggcggctgg tcaacgcgct ggtatggtga gttattgcgc
    15601 gatgacgcga tgatgagtgc ggttggttca agcctgacaa ttgctgcctg tgcggcaacg
    15661 gcggcggcga tcctcgggac tattgcggcg gtggtgctgg tgcgctttgg caggtttcgc
    15721 ggatcaaatg gctttgcctt tatgatcacc gcgccgctag taatgccaga tgtcatcacg
    15781 ggcttgtcgc tgttgttatt attcgtcgcg cttgctcatg ccattggctg gcctgcggac
    15841 cgcggtatgc ttaccatctg gctggcgcat gtcactttct gtacggctta tgtggcggtc
    15901 gttatttcgt cgcgtctgcg ggaactggat cgctcgatag aagaagccgc gatggatctc
    15961 ggtgcgacgc cgctgaaagt gtttttcgtc attacgctac cgatgatcat gcccgcgatc
    16021 atttctggct ggttactggc ttttactttg tcgcttgatg atctggtgat cgccagcttt
    16081 gtttccgggc cgggagccac cacgttaccg atgctggtct tttccagcgt gcggatgggg
    16141 gtgaatccgg aaatcaacgc cctggcaaca ttgatcctcg gcgcggtcgg aattgtcgga
    16201 tttatcgcct ggtatctgat ggcgcgcgca gaaaaacagc ggatacgcga catccaacgt
    16261 gcaagacgtg gctgaagaca ctaaaatttg ccaacctggc tacataatgc cgcgcatgtc
    16321 gcggcattgt tttcatggaa gacgaaacgt tgggattttt taagaaaaca tcttcatctc
    16381 atgctcgcct gaatgtgcct gcgctggtgc aggtggcggc gctcgccatt attatgatcc
    16441 gtggcctcga cgtgctgatg attttcaata cgctgggcgt gcgcggtatt ggcgagttca
    16501 ttcatcgcag cgtacaaacc tggagtttaa cgctggtctt tttaagcagt ctggtgctgg
    16561 ttttcattga gatctggtgt gcgttttcac tggtgaaagg gcgtcgctgg gcgcgctggc
    16621 tatatctgct gacacaaatc accgccgcaa gttacttgtg ggcggcttcg ctggggtacg
    16681 gttatccgga gctgttcagc attcccggtg aatcaaaacg tgaaatcttc catagcctga
    16741 tgctgcagaa gctgccggat atgctcatcc tgatgctgct gttcgttccc tcgaccagtc
    16801 ggcggttctt ccagttgcaa taatgtgtat aatcgtcgcc cctgatgatg tgaaggtcaa
    16861 tgtatgcagt gcgcacttta cgacgcgggt cgctgtcgtt cctgtcagtg gataacgcag
    16921 ccgattccag agcaactctc cgctaaaacc gccgatctta aaaatctgct cgccgatttt
    16981 ccggttgagg aatggtgcgc gccggtgtca ggcccggaac aagggtttcg taataaagcc
    17041 aaaatggtgg tgagtggtag cgttgaaaaa acactgctcg gtatgctgca tcgcgatggc
    17101 acgccggaag acctttgtga ctgcccgctt tatccagcct catttgcgcc cgtttttgcg
    17161 gcgctaaaac cgtttatcgc ccgagcgggg ttaacgccct acaacgtggc gcgtaagcgt
    17221 ggcgaactga aatacattct gctgactgaa agccagagtg atggcggcat gatgctgcgc
    17281 tttgtactgc gttctgatac caagctggcg caactgcgta aggcgctgcc gtggttacac
    17341 gaacaactac cgcagctgaa agttattacc gtcaatattc agccggtaca tatggcgatt
    17401 atggaagggg agacggagat ctacctgacc gaacaacagg cactggcgga gcgttttaat
    17461 gacgtaccgc tgtggatccg tccgcaaagt ttcttccaga ctaatccggc ggtcgccagc
    17521 cagttgtacg ccaccgcgcg cgactgggta cgacagctgc cggttaaaca tatgtgggat
    17581 ctgttctgcg gtgtgggggg ctttggttta cactgcgcga cgcctgacat gcagttaacc
    17641 gggatcgaaa ttgcgccaga ggccattgcc tgtgcaaagc agtcagccgc tgaactgggc
    17701 ttaactcgtt tacaatttca ggcgctggac tccactcagt ttgccactgc tcagggggag
    17761 gtgccggagc tggtgctggt taacccgccg cgccgcggca ttggtaaacc gctgtgtgat
    17821 tatctttcaa cgatggcacc gcgttttatt atctactcca gctgtaacgc ccaaaccatg
    17881 gcgaaagata tccgcgaact gccaggttac cgcatcgaac gggtacagct ttttgatatg
    17941 ttcccgcaca ccgcgcacta tgaagtgttg acgctgctgg tgaagcaata aaaaagccac
    18001 aggtgcggct tcagattgct gatcaggtgc gctttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaat catgctaaat caatacattg cagagttgat gtgggccgga
    18121 taagcgcagc gcatcaggca atcttacttt tgtcatcagt ctcaattact gcggaaacca
    18181 ctggtcattg attttctgat atgtaccgtc agctttaatt gctgccagcg cgttattcag
    18241 tttttccagc agagctttgt tatccggacg tacagcgata cccaggccgg tgccgaagta
    18301 ttgcggatcg gtcacttttt cagtcgcaac gcccagttgc ggattggttt tcagccattc
    18361 gtttaccacc gcggtgtcac caaatacccc atcaatacga ccatttttca gatcgataaa
    18421 ggcattctga taactgtcat aggagacagt tttcacttcc gggtgctgat cctgaatata
    18481 tttctggtgc gtcgtaccgt tttccatccc aatgcgtttg cctttcaggt cggcaaacgt
    18541 tttgtaggta tcttttttgg aaatcacgac ggctgagttt tcatagtagg gtgtggtaaa
    18601 cgaaacctgc ttgctacgct ccggggtgat atccataccg gagattacgg cgtcatattt
    18661 tctgaatttc agtgacggga tcaggctgtc gaacgcgtga ttagtaaaag tacattctgc
    18721 ctgcatttgt ttgcacaagg cttttgccag atcgatatca aagccgacaa tctcattatt
    18781 agcacctata gattcaaagg gtggataggt ggctgaaacg ccaaaattga ttttctctgc
    18841 ggcagaagca ccgaaagtaa aggaagcaag taaagcggca agaactaact ttttcatgat
    18901 ggaactcccg tctgtcaatc ttatgatttt tggccgtgtc tgcggcatgg gataacaatg
    18961 ccatcaagtg aatttatatg caataaacat gattaaataa tttaaatgaa ataaaaaaag
    19021 acggataact tagtgagttg tccgtcttca ttataagaat ttatgcacta tgtaggccgg
    19081 ataaggcgtc cacgccgcat ccggcacagg caccgtgctg atgtctgatg cgacgctggc
    19141 gcgtcttatc agacctacaa acccccccgg cgaatgtacg cagctacatt aattccgccg
    19201 ttcgaatgcc agtgctttgc gctcgatcag acgcatcatc agcgtcagca ggccgttgac
    19261 gaccaagtaa ataatccctg ccgcaccgaa caccattaca tcgtaggtgc gtccgtacaa
    19321 caactggctg tatcccatca cttccatcag cgtaatggtg taagccagag aggtactttt
    19381 gaacaccagc accacttcgt tggaataaga agagagcgag cgtttaaagg catacggcag
    19441 caggatcgcc agcgtatctt ttttgctcat tcccagggcg ctacaggact gccactgacc
    19501 ttctgggatc gcacgaattg caccgtaaaa cagctgcgtg gtatacgccg cactgttcag
    19561 cgataacgca atcagcgcac ataaccacgg ttctgacaac aaatgccaca gtgccggata
    19621 ctcctgcaac gtcggaaact ggcccggccc gtaataaatc aggaagatct gcaccagcag
    19681 cggcgtaccg gtaaacagcg tgatataacc ccgcaccagc cacaccagca ccggcgtttt
    19741 cagcgtcagg atgatggtaa aaatcaatgc cagaatcagt gccacaatca gcgaggcaac
    19801 ggttagcgtc aggctggtgt gcagcccttt catcagctcg ggtaaatact caaacattag
    19861 ctgggcctcc gctcaaaacg tgtcgcgcgc aggtcaatgc gtttgagaat gtactgactg
    19921 agcagggtga tcaccaggta aatcgccgcc gccacaatgt accaggtaaa tggttcctgg
    19981 gtacgagtag caatgctttt ggtttgtaac attaaatcat tcacactaat caaactgacc
    20041 agcgcggtat ctttcagcag caccagccac tggttaccga ggccaggcag cgcatgacgc
    20101 cacatctgcg gcatcaccag acggaaaaag atagccgatt tcgacagccc cagcgcctga
    20161 ccagattccc actggcccac cggtacagct ttcaacgcgc cgcgcagcgt ttgcgaggca
    20221 taggcggcat acagcagtga cagagcgatg acaccacaaa ggaacggact cacgtcgaag
    20281 ttctcaatgt ccatctgcac cgggatctgc acgaacccaa gattgatagt gaagccatcc
    20341 gaaagcgtca gcagtaactg cgaggagcca aaatagataa acagcactac cagaatttct
    20401 ggcaggccac gcagaatggt taccagcgct gaacctgccc acgcgacagg acgccatttc
    20461 gctgactccc ataccgcaaa gaacatcgcc agcgccagcc cgacaatcaa tgcacaaacg
    20521 gcaaggccga cggtcatccc ggcggcgctt gctaaaggaa aaaattcatt catcaggaat
    20581 tacttctgga accatttgtt gtagatggtt tcgtaagtgc catctttctt cactttttcc
    20641 agcgcagtgt tgagtttctg ctgcagttca gtgtttccct gacgtaccgc gatgccgagg
    20701 ccagtaccga aataatcttt atcggtcact ttatcgccca ctgccgccag ttttgggtta
    20761 tctttcagcc actcagtgac cactgcggtg tcaccaaata cgctgtcgat acgcccgttt
    20821 tgcagatcca gttttgcgtt ctggtagctg tcatacggaa cggtggtgat ttccgggtgc
    20881 ttatccataa tgaacttctg gtgtgtcgtc ccgttctgaa cgccgacttt tttgcctttc
    20941 agctgatcaa cactggtgta tttgccttgc tgacccacaa acagggcaga gttgtcatag
    21001 tacggggtag taaacagtac ctgcttttca cgctccggag tgatatccat gcccgccatc
    21061 actgcttcta cgcgacggaa tttcaggctt gggatcaggc tgtcaaacgc ctggttagag
    21121 aaagtacagg ttgcatcaat ctctttacac agtgcctgcg ccaggtcgac gtcaaaacca
    21181 acgatctggt tgtttgcatc aatcgattca aacggaggat agggggcttc ggtagcaaaa
    21241 cgaatggttt cggcagctgt ggcggaaaga ctaaaacctg caattaacgc ggcaatcaga
    21301 acttttttca ttgttgttat cccgaatctt agtgagagag atagttttta aatgcttcgg
    21361 tttgcggctc ggtaaagcag ctcgcgtcgc cttgttctac gatatgacca ttttccatat
    21421 acaccactcg gctggcggtt ttacgcgcca cttcaacttc gtgggtgacg atcacctggg
    21481 taatattcgt ttctgccagc tcacgaatga tgctgacgat ttgtgccgta atttccgggt
    21541 ccagtgcggc ggtcggttca tcgaacagca gtacctgcgg ttccatcatc aacgcacggg
    21601 caatagcaac acgctgctgc tgaccaccag aaagatgcag cgggtaacga tcgctataag
    21661 gtttgagacg cagacgttcc agcagttttt ctgcacgggc cagcgcctga tctttactca
    21721 accccagtac acggcagggc gcttcaatca ggttttgctg cacggtcaga tgcggccaca
    21781 ggttgtattg ctgaaacacc atgccaacgt tacgacgcaa atcgcgaatc gctttgtcag
    21841 agggtgtttt ggtgaaatcg aaatggttgc ctgcaatgtt gagcgtaccg gagcgcggca
    21901 tctcaagcag attgagtaca cgcagcagcg agcttttacc cgcgccgctg gggccaagta
    21961 acaccagcgt ttcgccctgt gggcaatcca gcgtgatatc gaacagcgcc tgatgcgcgc
    22021 cgtagaagca attaatgccg tttaattgaa tactcattga cactcgtata ctggcagtct
    22081 gatagctatt gaggtcgaag atagtacctt taacagaata attatgcaat atttatgctt
    22141 taaaagttaa aagcaaagcg cattattcaa taaacatagc acaaaataac gggggcggtg
    22201 gtcggtgagc ataaatgtcg gcattcctca cgaaatgccg gataatttac ggggtttatt
    22261 ggttgatcaa ggcgttagcg attctcgatg gactgacgga gcgtacccgc cgtggcatga
    22321 acgctaccgc ctaagtagcg cacatcgtcg atgacccaac actggccttc ctgaatcatt
    22381 aacacttcat cctgccagcc ctggtcaccc tgtttgagat ccacccgcaa tggaatgtta
    22441 cgggcatcac gattagggat agtcgatgca ctggcaacgt gggcgctgtc tggcaaggtg
    22501 gttcgactgg agaacggatc gctgcttaac aattcacgat gactattatc gcggctggca
    22561 tcgctaagtt gggttgccag tttgtcactc aaataggggc gcagggcggt aatgtcattg
    22621 ctgcgatgtt gaatgcggta gtcgtagaat tgttgagcca cactgtcagg ccctccctgg
    22681 acacaggaac cggtgcgggg gccgttatct ttataagctg gcgtgacggt ggtacaggca
    22741 ctcagcagca gtgcacatgg aataagtaga gatagtgaac ggtagcgcat aatcatttcc
    22801 ttattagctt gctgtctgcg tcacactacg accaaacgct atcagattat ttaatacatt
    22861 gtttttaata caattctgaa acaatctgaa cacctgattg ggtttgagtc cattgctggc
    22921 gacccactca aggttgctcg tagctgactt acttaaatta cccaaacaat agacagtgta
    22981 aaccatttgt gaggttgagc gtgttatatg gctgaaatga tcatttaagg taaatatgat
    23041 agttctgaaa tggtggtata aatcctcctg tagaggttaa gagcatctgc aagaagccag
    23101 tttcacattt ataacgcgtg ataaatgaat gcggttattg tctaatcatt acaaaatttt
    23161 tcacatgcga taagagatcc tgcgattaag atgattgcca aaattacgtt gtatttttga
    23221 aaatgagcga aatttgatag tcccagcttt gactctcgat aatattattt atgctacaat
    23281 tgtgggtaaa gaatttaagc taatgaattt gagtgaagaa taaattaaat tgcttcagta
    23341 tttcgttaat taatataaat acaaaaggcg ttgtgctttt ataattataa agcgcgagga
    23401 gttcggtgga ttat
//
LOCUS       DAZOZX010000055        22837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZOZX010000055 DAZOZX010000000
VERSION     DAZOZX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.055"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            124..1146
                     /locus_tag="ACHUPT_002136"
     CDS             124..1146
                     /locus_tag="ACHUPT_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR0819706.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1146..2126
                     /locus_tag="ACHUPT_002137"
     CDS             1146..2126
                     /locus_tag="ACHUPT_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR0819707.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2123..2881
                     /locus_tag="ACHUPT_002138"
     CDS             2123..2881
                     /locus_tag="ACHUPT_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0819708.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            2891..3700
                     /locus_tag="ACHUPT_002139"
     CDS             2891..3700
                     /locus_tag="ACHUPT_002139"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0819709.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3700..4554
                     /gene="modD"
                     /locus_tag="ACHUPT_002140"
     CDS             3700..4554
                     /gene="modD"
                     /locus_tag="ACHUPT_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR0819710.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            4580..6534
                     /locus_tag="ACHUPT_002141"
                     /pseudo
     CDS             4580..6534
                     /locus_tag="ACHUPT_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(6584..6838)
                     /locus_tag="ACHUPT_002142"
     CDS             complement(6584..6838)
                     /locus_tag="ACHUPT_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0819711.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7039..7773
                     /locus_tag="ACHUPT_002143"
     CDS             7039..7773
                     /locus_tag="ACHUPT_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0819712.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7775..8386)
                     /gene="emtA"
                     /locus_tag="ACHUPT_002144"
     CDS             complement(7775..8386)
                     /gene="emtA"
                     /locus_tag="ACHUPT_002144"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0819713.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8486..9400
                     /gene="ldcA"
                     /locus_tag="ACHUPT_002145"
     CDS             8486..9400
                     /gene="ldcA"
                     /locus_tag="ACHUPT_002145"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0819714.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            9495..11231
                     /locus_tag="ACHUPT_002146"
     CDS             9495..11231
                     /locus_tag="ACHUPT_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0819715.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11842..13256
                     /locus_tag="ACHUPT_002147"
                     /pseudo
     CDS             11842..13256
                     /locus_tag="ACHUPT_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13424..14494)
                     /gene="dadX"
                     /locus_tag="ACHUPT_002148"
     CDS             complement(13424..14494)
                     /gene="dadX"
                     /locus_tag="ACHUPT_002148"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0819716.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14504..15802)
                     /gene="dadA"
                     /locus_tag="ACHUPT_002149"
     CDS             complement(14504..15802)
                     /gene="dadA"
                     /locus_tag="ACHUPT_002149"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0819717.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16132..17664
                     /locus_tag="ACHUPT_002150"
     CDS             16132..17664
                     /locus_tag="ACHUPT_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0819718.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(17716..18435)
                     /gene="fadR"
                     /locus_tag="ACHUPT_002151"
     CDS             complement(17716..18435)
                     /gene="fadR"
                     /locus_tag="ACHUPT_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0819719.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18656..20197
                     /gene="nhaB"
                     /locus_tag="ACHUPT_002152"
     CDS             18656..20197
                     /gene="nhaB"
                     /locus_tag="ACHUPT_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0819720.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20343..20873
                     /gene="dsbB"
                     /locus_tag="ACHUPT_002153"
     CDS             20343..20873
                     /gene="dsbB"
                     /locus_tag="ACHUPT_002153"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0819721.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20919..22187)
                     /gene="umuC"
                     /locus_tag="ACHUPT_002154"
     CDS             complement(20919..22187)
                     /gene="umuC"
                     /locus_tag="ACHUPT_002154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0819722.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22187..22606)
                     /locus_tag="ACHUPT_002155"
     CDS             complement(22187..22606)
                     /locus_tag="ACHUPT_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0819723.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5462 a         6004 c         5714 g         5657 t
ORIGIN      
        1 gtcaacaggc ttgcggaagt cgtcacatct tcatgttttc aatagctaaa ctttgatatt
       61 gaacatgatt tttatcgata tatatgaaag tatatatcga ttttactttt caggatgttc
      121 accatgccca taaccagacg gacgtttgcg caggcacttg catctaccct gctcctgcag
      181 tctctaccgt ccttttcaca aacagttaat cgttttgctt cgcaatctct tcccgaagcc
      241 caaaacatta cacgcatcgt cagcgcaggc gcgcccgccg atttgctctt gctggctgtc
      301 gcgccagaaa aaatggtcgg tttttcctct tttgattttg cccgtcaggc attaattcca
      361 ttgccagagc acattcgcca gttacccagg ctgggacgac tcgccgggcg cgccagcaca
      421 ctatcgctgg aagggcttat ggcgttacat cccgatttgg tcgttgattg cggcaatacg
      481 gatgaaacct tgatctccca ggcacggcag gttagcgaac agacacaaat cccctggtta
      541 ttgcttaacg gaaaactggc gcaatcagca gaacagttaa caacgcttgg caaaacgtta
      601 ggcgaagagc accgcgccgc agaacaagcc aatctcgcca gccacttcgt tggtgaagct
      661 caggcattcg ccacctcacc cgccgctaac ctccgctttt atgctgcgcg cggtccccga
      721 gggctggaaa cgggcttaca gggttcgttg cataccgagg cggcggaatt attaggtttg
      781 cacaacgtcg cgcaaatagc cgatcgccac ggcctgacac aggtttccat ggaaaatctc
      841 ctgcgctggc agccggatat tattctggtt caggaggccg ttactgcaga ttttattcgt
      901 cgtgatcctc tctggcaggg cgtgaaagcc gttgcggaac aacgcatctt atttttaagt
      961 ggcttgccct ttggctggct cgatgccccg ccgggaatca accgtcttct gggattacgc
     1021 agacttcacg cctggctgga tcccgccatc aatcgccagt ttaaaagtga catgcagcat
     1081 tacgcccaac tgttctggca ttgttcactc agtgatgccg actatcaaaa attggtggcg
     1141 agctaatgag aatcgtaaac ggatgtattt tgctgctggc agctatcagt atcacttttg
     1201 ccgccgtaag tggggcttat catctcgata tgcagcaact gctggcgcta attcttcgtc
     1261 aagaaaatga gtctgtgcag gaacaaattg tcttctggca aatccgcgtg ccgcgtattc
     1321 ttgctgcgct gttactgggc gcagcgctgg caggagccgg aaccacctat cagggaatgt
     1381 tgcgtaatcc cttagtttca ccggatattt taggcgtctc cgccggtgcc ggacttggtg
     1441 cctgcgcggc aatcctgtgg ggattaccca ttgtttacat ccagctgtac gcattttgcg
     1501 gcgggctgat ggtggtcgcg ggcgtctggt tgattacgcg tcgggtgacc cgccacgatc
     1561 cgatcttaac cctggtgctg gtaggcattg cgttaggaac gttgtgcgga gcggggatat
     1621 cgctaattaa aacgctggcc gatccttaca cccagttacc gtcaattacc ttctggttgc
     1681 tggggggact gtccaccgtg accttacgcg atctctgcta tgctgcaccg atcattctca
     1741 taggctcctt gcccctgttt tttctccgct ggcgcatgaa tttactcacg ctttccgacg
     1801 acgaagcccg ttcgctggga ctgaacgtga cgcgtctgcg ctttggattg attgtctgcg
     1861 ccacactaat caccgccagc acggtagcaa tagcgggcat aattggttgg atcggtctgg
     1921 tcgttccgca cattgcccgc ttgttgaccg gtcataacca tcagcagctg ttgcctatgg
     1981 caatgtgtac tggcgctatt ctgctcctgc ttaccgatac gctggcgcgc agcattggaa
     2041 caacagaaat cccacttggc attctgaccg cttttgttgg cgcaccgttt ttcctgcttt
     2101 tgttgttacg cggaggtcgc cagtgacttt gcttgccgtt cgtcatgcgt ctctgggtta
     2161 ctcccgccat ccagtgctac gcgacgtgtc cttcaccctg tcgcaaggaa caatttgttg
     2221 tctgcttggc gcgaatggtt gcggcaaaac cacactcatg cgctccattc ttggcgttat
     2281 accgttatta aaaggcgaga tcctactgga tagcctctct gtgcagacac tcagccatcg
     2341 ccagcgcgca caggctattg cctgggtgcc gcaggcgcac gacggtattt ttgctttcag
     2401 tgtgctggat atggtgttga tgggcctggc ccccaccata ggcgcttttt ccgttcccgg
     2461 taagcaagaa cgcctgaaag ccacggagca actggaaaag ctgggcattt tgcatctggc
     2521 gacacgacgc tggaatacgc ttagcggcgg tgaacgtcag ctggctttaa tcgcccgtgc
     2581 actcgtccaa caaccgcgct tgttgttact ggatgagccc gcctccagcc tcgatttcgg
     2641 tcatcagatc caacttctgg acacgctggc gcagctcaaa aataacggca tgacaatgtt
     2701 aatgtcgact caccatacgc tccatgccaa cgccattgcc gacagcatta tccaagtaga
     2761 gccagatgga cgagtaacac aaggcttacc gaccgaacag ttaacaacga acaaactggc
     2821 ggccctgtat cgggtttctg ccgaccaaat tcatcatcac ctctctgcaa ttagccgcta
     2881 aggaacaatc atgctcattg atgatattga ttttgcagac ctttatctgc agcaactcaa
     2941 actggctcac cgcacagaaa aaacgcccga tcactgggat caacgggcgg agaaaatggc
     3001 ggaaaattgc gcctcgccaa cggacagcta cctgcaacaa ctgatcgcta aaattgacct
     3061 gcaaggcgct cagaccttat tcgatatggg ctgcggacca ggcaccgtat cgctggcgct
     3121 ggctgacaaa ctcactacgg tttatggcgt tgattacagt caggggatgc tgaacgttgc
     3181 cgcccgccgc gccgccgcac tgaaggctga caatgtccac tggatccagc gcgcctggga
     3241 agaggactgg agtgacctgc cacgctgcga tattgccgtg gcttcccgct ccactctggt
     3301 ggctgatatg cgccatgcaa tgagcaaact gaataatcag gcacggcttc gcgtctatac
     3361 aactcatctg gtcagcacct cgttcgtctc tccagccatt cagcgggcgg ctggtcggga
     3421 agtgatcgaa ttacccaact atatctttgc gctcaacgtc ctgtatcaga tgggaattta
     3481 tgcccacgtt gattttatcc gtggacaaaa ttgccaacag gataacagca cctgggaacg
     3541 ttttgagcag aacgtcagct ggagtcttgg cgcgcttaac gacgatgagc gtgagcggtt
     3601 atatcactgg tatcaacaac aagatgctcg tgccctcgcc cctgccagcc gcgactgggc
     3661 gctcatctgg tgggatagcg taccgcagga ggcactgtga tgattttcct ttctcaggca
     3721 caaatcgatg cgttactgct ggaagatatc cagggaggtg acctgaccac gcgagcatta
     3781 aatattggac accagcctgg ctatatagcg ttttttcacc gtcagggcgg ctgcgtcagc
     3841 ggcgtttctg tcgcgtgtaa gatgttaact acgctgggat taactattga tgacgcggtc
     3901 agcgacggtt cacaagcgaa cgcgggtcaa cggctaatcc gtgcgcaggg taatgccgca
     3961 gcacttcatc agggatggaa ggcagtccag aatgtgctgg agtggagttg cggtgtttct
     4021 gattatctcg ctcaaatgct ggcattgctt cgtgaacgtt atcctgacgg caatatcgcc
     4081 tgcacccgaa aagcaattcc gggcacccgc ttactggctt cgcaggcaat tctggctgcc
     4141 ggaggactaa ttcatcgtgc cggatgtgcg gaaaccatat tattgtttgc caaccaccgc
     4201 cattttcttc atgacaatca gggctggtca ggcgcaatcg ctcagttacg tcgccacgca
     4261 cccgagaaga aaattgttgt tgaagccgac acgccgaaag aggctatcgc cgcgttacgc
     4321 gcacaaccag acgtacttca gctcgacaaa tttagtccgc aacaggcaac tgaaattgcg
     4381 caaatagcgc cgtcgctggc tcctcactgc acgctggctc tgaccggcgg aattaatctg
     4441 acgacactca aaaattacct cgactgcggc attcgccttt ttattacctc cgcgccttat
     4501 tacgcggcac cagctgacat taaagtcagt ctgcaaccca cagcctctat ttaattcttt
     4561 gttaaacgga atttattcta tgagactcaa aaaacgttat ctctgcacag cattatcact
     4621 tgcctttacc cagcaggccg tagcggctca ggagagcgac acgctgaccg tatggtccag
     4681 tccggtatca tcgacgacga ccaccgttct cgatcaaccc accatgaagg ccttggataa
     4741 acagaatgtc gctcaggcat taagtgtcgt ccccggcgtg gtgctgcaaa agtcaggcag
     4801 ccgcaacgaa gaacaggtaa aagttcgtgg ctttgatagt cggcaggtgc cggtctattt
     4861 cgacggtgtg cccatttatg tcccctatga cggcaacctc gatctggcgc ggattctgac
     4921 caacaatctg ggggcagtcg aagtttccaa agggtattcg tcgctgcttc atgggcctaa
     4981 tcagatgggc ggagccatta atatcaccac cctgaagaag caagtctggg atatcgccag
     5041 ggatggagcc gtagtcagga caatgcctac gatatgcatg cttcatttgc cgccagcagc
     5101 gatcttgggt atttgcaagt cagcggtagc cagctaaagc aggattttct cggcctgccg
     5161 catggtgtaa ataatgatat tgcaggcaaa cacggcaaga tgattaattc atcggctgat
     5221 gataaacgcg gcattgtgaa gctcggtttt acaccgcgcg aaaacgatga atacacgctg
     5281 acttacatta agcaggatgg tgaaaaagat aacccgccat acagcggaaa ttgtggtcaa
     5341 aaatcacgct actggcagtg gccagagtat gacaaagaaa gtttttatta tcagggagcg
     5401 acccaactaa acgaccgttt taccctgaaa agtcggctgt atcgcgacac ctttgaaaat
     5461 acgctgatga tgtacaactc gctggctgat ttgaaaaata aaaaaggcag ctacagccat
     5521 tattccgatt acagcgacgg tgccggatta caactggcag ccgatgtgcg tgaaaacgat
     5581 ctgctgtcgt ttgccgttaa ctggaaagat gacgtacacc gggaaaaagg tgcgccgcac
     5641 gccgcttacg atcgctatga agatcgtacc tggtcgctcg ccagtgaata tcaatgggct
     5701 gctgctgata atgtcgatgt cgtggcggga atcagctatg actggcgcga tagcgtagaa
     5761 gcgaaaaaac acgagaaaga tggcagcatc acccattatg acgacaacaa tcagtcagct
     5821 tttaactggc aggtgatggg gaaataccac tttgccaatg aagacactct ggcgctttcg
     5881 tactatgacc gcacacgctt tccgacgctg aaagaacgct ataccacatc caaacctgcg
     5941 tataaccaga tagcgattgt taacccgcag ctcaagccgg aacgcgctcg cggggtggat
     6001 ttaacctgga atggtgcctt cacgcgcgac tggggatttg aggtcagcgt ttactataac
     6061 cgggtgagtg atgccatcct ctcgcacaat atcaatgccg ataccattca aaatcagaac
     6121 agcggcacgg tggattacag cggtctggat gccggtatta aggggaaaat cagcaatata
     6181 ctggatgtag gattgagcta cgctctgatc cacgctgacg ccaaacgcaa agacatcggc
     6241 aagataaccg atcttccaac gcagacaatg accgcatgga tgacactcaa accatgggag
     6301 ccgttaagcg taacgctgtc ggaagaggcg cgttcctcca gctacagcaa cagtgacggt
     6361 tcacaaaaag ccgccggttt tgcggtgacc cacattcgag ccgattacac cttaggtcat
     6421 ggcttcagcg tcaatgcgtc ggtcaataac ctgtttgata cccaatatgc ctacagtgaa
     6481 gggtttattg aagaaggtcg caatttctgg gcaggtgtcg agtatacgtt ctgatgtgaa
     6541 agcggatagc ggcatctgta tcgctatccg ccttatgaaa atcttattct cttcgcagga
     6601 ggcggaatac gcccagcacc aggatagcgc ccaccaccgc caccaggaag ctgtgcaaat
     6661 taaaaccgct gatggagccg tcaatgccaa acatggtcgc cagccagccg ccgaccaccg
     6721 caccgactat cccgagaata caggtcagga aaaatccacc accatcacgc cccggcatga
     6781 ttagcttgcc gataatgccg gctatcaggc caaaaataat ccaggcaata attcccatgt
     6841 tcaggccctc gtttcgatga atcggtcgca agaaatgctt acgtcaacgc tctaagtata
     6901 ggcaagggcg ggatcgatgc tgggcgtttg tcggctgatt taagaacaac atgtaaaaaa
     6961 tattcacgca tttgtattaa gttttgttaa ctatgaccga taaaccaaag acagtttgtc
     7021 agtcaggagt ttttccgcgt gagtcattac catgagcagt tcctgaaaca aaatccgtta
     7081 gccgtcctgg gcgtgttacg cgatttgcac aaagccgcaa ttcctttgcg tctcagttgg
     7141 aatggcgggc agctgatcag caaaatactg gcaataaccc cggataaact ggtgctggat
     7201 ttcggcagtc aagccgaaga caacatcgcc gtgctaaagg cacagcacat taccattacc
     7261 gccgaaactc agggtgcgaa agtcgagttt actgttgaac aactacagca gagtgaatac
     7321 ttgcagcttc cggcatttat taccgtaccg cctcccacct tatggtttgt acaacgacgc
     7381 cgatatttcc gcatctccgc cccactccat ccgccttatt tttgccagac caaactggcg
     7441 gataacagta cgttacgttt ccgcttgtat gatttgtcgt taggcggcat gggcgcatta
     7501 ctggaaacag caaagcctgc cgaattacaa gaaggtatgc gcttcgctca aattgaagtc
     7561 aacatggggc aatggggtgt ttttcacttt gacgcccagt taatctccac cagcgagcgc
     7621 aaagtgattg atggcaagaa tgaaaccatc accactcccc gtctgagctt ccgttttctt
     7681 aacgtcagcc cgacggtgga gcggcaatta cagcggatta ttttctctct cgagcgagaa
     7741 gcccgggaaa aagcggacaa agtgcgcgac tgatttacat cgcgtccagt gcctgctcaa
     7801 gtttgtagat atagcgcgga gcctgcggcg caggatgatt tcgcgctacg tgatcgagga
     7861 actcgtcagc atccaaatcg ttgattttgc tgatcgcctt tttccgatct gacgagaaag
     7921 tccgtagcag cgcacctgcc ccgttagcgt atgacaccac cagcgcatat tgcagtacct
     7981 tcggatcttc aatgcctgcc agcgggccgg tttccagaat attcaggtaa gccgccccca
     8041 ttgaaatatt acgctctgga tttttcagct cgctggtcgt cggctcacca ctccagccca
     8101 tacggcgata aacatcacgt ccggaggttg aagcttttaa ctgcatcaaa ccaatggcat
     8161 tcgatttact caccgcgttc ggattaccac ccgattcgat agcgataatc gccgtgatca
     8221 attgtggatc gacgccccag gctgcaccgg ctttctggct tattggcatc cactgcatcg
     8281 cacgttgcac cggaactttc gcgttccacg gcgggttcgt atagtcatgc tttgatgaac
     8341 aacccgctaa taacacaatc aaaaaggcaa accatctcaa tttcacttct tctatcctta
     8401 tagcctggaa tcgccgttga ggtgacaacg gcacacctaa gcggcatgat atacatttcg
     8461 tttctgttgt cagcaaggaa ttgccatgtc tctgtttcac ttaattgccc catcgggtta
     8521 cagcattaaa cagcacgccg cgctgcgtgg tatccagcgc cttaccgacg cggggcatca
     8581 agtcaataac gtcgaggtta ttgcccgtcg ttgtgagcgt tttgcgggta cggaaacgga
     8641 gcgtctggaa gatctcaatt cccttgccag actgactacc cccaacacca tcgtgctggc
     8701 tgtacgcggc ggttacggtg ccagtcgttt actggcagat attgactggc aggcactggt
     8761 ggctcgccaa caacatgacc cgttactcat ttgcggacat agcgatttta ccgccattca
     8821 gtgcggtctt ctggcgcaag gcaatgtcat cacctttagc ggccccatgc tggtagcaaa
     8881 ctttggtgcg gatgaactga acgcctttac cgagcaccat ttctggctgg cgttacgcaa
     8941 tgaaaccttc accattgaat ggcaagggga aggcccgaca tgtcagaccg aaggcacgtt
     9001 gtggggaggc aatcttgcga tgctgatttc actgattggt acaccgtgga tgccaaaaat
     9061 tgagaacggt attctggtgc tggaagatat taacgtgcac cctttccgcg tcgaacgtat
     9121 gctgttgcag ctctatcatg cgggaatttt gccacgtcag aaggcgatta ttctcggtag
     9181 ctttagcggc agcacgccca atgattatga cgcaggttac aacctggagt cagtgtacgc
     9241 gtttttgcgt tcccgcctgt cgattccact cattaccggc ctcgattttg gtcatgaaca
     9301 acgcacggtt acgctgccgt tgggcgcaca tgccattctg aataacaccc gggaaggcgc
     9361 tcagttgacg attagcggtc atcctgttct taaaatgtaa aaaaatcacg cttcagggct
     9421 aagtaaaacg ctgtctctgc cgctaatatg gtagggttta tataagagac agcgtaatca
     9481 ggagtaaccg accattggat gccacaacaa taattagcct tttcatctta ggatccatcc
     9541 ttgtcaccag cagtatatta cttagttcat tttcttcccg tcttggcatt cctattctgg
     9601 ttatttttct ggcgatcggc atgctggcgg gagtcgatgg cgtcggcggt atcccgtttg
     9661 ataattaccc cttcgcctac atggtaagta acctggcgct ggcgattatt ttgctcgacg
     9721 gcgggatgcg cactcaggcc agttcctttc gtgtggcgtt aggaccggcg ctgtcgctgg
     9781 cgacgctggg cgtacttatc acctctggtt taaccggcat gatggcggcg tggctgttta
     9841 atcttgattt gattgaaggc ttattaatcg gcgctatcgt cggctccacc gatgctgcag
     9901 cggtcttttc tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg acgctggaaa
     9961 ttgaatccgg cagtaatgat ccaatggcgg tctttctgac gattaccctg attgcaatga
    10021 tccagcagca tgaaagcagt gtcagttgga tgttcgtggt cgatattctg cagcaatttg
    10081 gcctcgggat tgtcatcggt cttggcggtg gttatttact gctgcaaatg attaatcgca
    10141 tcgccctgcc tgccggatta tatccattgc tggcattaag cggcggtatt ttaatttttg
    10201 ctttaaccac ggcgctggaa ggcagcggta ttctggctgt ttatctgtgc ggttttctgc
    10261 tgggtaatcg gccgattcgc aaccgctacg gcatcctgca aaatttcgac ggcctcgcct
    10321 ggctggcgca aatcgccatg ttcctggtgc tggggctatt ggttaaccca agcgatctgc
    10381 tgcctattgc cattccggcg ctcattttgt cggcatggat gatattcttc gcccgtcccc
    10441 tttcggtatt tgctggattg ctccctttcc gcggtttcaa tctgcgtgag cgcgtgttta
    10501 tcagctgggt gggattacgc ggcgcggtgc caatcatcct agccgtgttc ccgatgatgg
    10561 cggggctgga gaatgcgcga ctgttcttta atgtcgcctt ctttgtggtt ctggtttcac
    10621 tgctcttgca gggaacatca ctctcgtggg cggcgaaaaa agccaaagtg gtggttccgc
    10681 ccgtcggacg tccggtgtca cgcgttggcc tggatattca tccggaaaat ccgtgggagc
    10741 agtttgttta tcaattgagt gctgataaat ggtgtgtagg tgcggcactg cgcgatttgc
    10801 atatgccaaa agagacgcgt attgcagccc tgtttcgtga taaccagttg cttcatccta
    10861 ccggcagcac ccgactgcgc gaaggcgatg tattgtgtgt aattggtcgg gaacgcgatc
    10921 tcccggcgct cggtaaactg ttcagccagt cgccaccggt cgcgctggat caacgcttct
    10981 ttggtgactt cattctcgaa gccagcgcca aatatgctga tgttgcgctg atatatggcc
    11041 tggaagacgg gcgagaatat cgtgataaac agcaaacgct gggtgaaatt gtccagcagt
    11101 tgctaggcgc ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg atctggacgg
    11161 tggccgagaa agaagataat gaagtgttga agattggcgt tcgggtggct gaagaggaag
    11221 ctgaatctta aatttaatgc agcatctatc gccggatgcg atgctttact gaacgcagcc
    11281 tgagcctgta ataaacttgt catttcctga ttttgatatg ctcatcccaa catcagaaga
    11341 aggttaaatt atggatgaca aaccattgca ggctctggct aatgaactca aaacaccggc
    11401 tcaagaaagt cagcgtaagg gctttttccg actgtaacag gtaataaaaa ccagccccgc
    11461 attaccggtc aaccatcagg ggcccctgcc tgacatcgcc tgaacatatt cccccacgca
    11521 acctgccgca ctgacatagc ccacacaata ggcgtttcat ccgcctgtag caccggatgc
    11581 atgagcgatt tcttatagtt attcctttgc ttaataacga ttatttccgg gtcgttcact
    11641 ctcgtatttt ctttaatacg tcagagctac ctaggccgta atacagcatg taccagaccc
    11701 gacacggcat ttctgaccag gaaaaaagtc aacgatgagt agtgatatca gcgcaaatga
    11761 cgtcctgtgg ctcctttgcg gtgaccacat tcacgtctcc ggcgctgcca gaggatattt
    11821 atttcaagga aacgtagcat tatggctagc tttaaagaca tctgcccatt cgcccccgcc
    11881 attcccccat cttccgttaa cccaggcgta cgacgcccgc cagggcggct cttctcaaga
    11941 atcaaatttt gcagaacatg aagtatctca gcccgcaaaa attcagctgg ggtgatgccc
    12001 cctggcagat cattgacctc agtatcgcgg gcaaagtgaa catacaggtg gacaacaata
    12061 ccatcatcac gttggggact cgtctgaatc agcaacataa cgagttcatg atggtcgcca
    12121 aatggtgcga atgggctata cagcaggatg gtttgcaaga aaacctgcaa aaaaacctgt
    12181 acgaaatcct ggaagaaaac cagcaaaaca aacagtcaga aattccccaa gaagacctga
    12241 aagaaagtct ggaagaaatc aaggaaaata tcctggaaga aaacctgcca gcaagccgga
    12301 ttgaaaaccg ggcagaagca ttgcgcagaa tgaaggaatg tctcattaca agacggagta
    12361 tgctcaatct tagcaacctg ggattgactt cactccctga gaatttgcct ccacatctga
    12421 ttgaatttta ctgcagtaaa aacgtgttga ccgcgttacc gaaggtgatg ccaaagtggc
    12481 tgctggtgct tgactgtaca gataacgtct tgatcttgtt accgaaggtg cagccctcaa
    12541 aactgatggt actgaagtgc tatgaaaaca gtattatctg gctgcctgag ctgtcgacta
    12601 acctgagagt gattaactgt tctgaaaact tcttgcaatt tttaccgccg tcgatgcccc
    12661 agtacctgta taaactatcc tgtgccggca acaatattaa ctcaatacct gatgagatgc
    12721 tggagaactt gactcgcctg aaagtatttg actgttctag taacgatttg atctcttcac
    12781 cacggctgcc gcccaaacta ataatttact actgtggaga aaacaagttt aaaacggtac
    12841 aggtgccgca gccccagagc ctgaaggtgt ttgactgtaa tggtaacccg tgggacaaag
    12901 acaatttacc gacgctgctc aaagccgtcg agggcctgaa aaaacagcag ggcctgaagg
    12961 accttttgga ctttttgcac aaggaaggct aggttgacct ggaaggactc gaggagctgg
    13021 aggaccttat ggatctggag ttcctggatg acccggaact cctggagcgc gtgaaggtac
    13081 aggaggacct ggagctcctg gatcaacagt tgggcctgtt gagtctggaa agacaacagg
    13141 actcgcagcc tgttaatcaa caatctgaac atgaacccga atctgcatca agggtgaagc
    13201 gtgatttatc tgaagtcgac tccgagtcaa caatgaagcg taagcgtttt atgtagaacg
    13261 cctgtgcggc gagttcaggt tccggggaaa tagtgtccat tcttttaatg gacactatca
    13321 tgggcaatgg ctctgtcaca ttaacaacta cagttgctga ccagccggat ggatgatttt
    13381 ttcgcaacca gaagacgctg cctccgatcc ggcttacaac aagttacact gtcacaaccg
    13441 ggacgcgtaa cgccagtgcg cacatcaact catagcccac cgttccggca gcggcggcga
    13501 catcatcaat tttgatctcc ttgccccaca gctcaaccgg cgtaccaatc cccgcctgcg
    13561 ggcaaggcgt taaatcaacc gccagcatat ccatcgagac ggtccccacc gtcatggtgc
    13621 gcacgccgtc cactaaaaca ggagcaccgg taggcgcatg gcgcggataa ccgtcggcgt
    13681 atcctgcggc gacaatgccg attcgctgtt catcgcgcgc agtatagcga ccgccgtagc
    13741 ccacacgctc gcccgctttt agcgtctgga caccaataat ctcactgctt agcgtcatca
    13801 ccggacgtaa tccggtattg gcgatatcac gccactgacc ggacggcgaa gcgccataca
    13861 aaataatgcc aggccgaacc cagtcaaaat gcgcttccgg gtgccacaga gtcgccgccg
    13921 aattggacaa cgaacgccga cactccagcc cctccgccgc ctgctcaata cgcgccatcg
    13981 cgccggaaat tccatcagga tgttccgctt cagcaaaatg cgacatcagg gtcatttcgc
    14041 caacattcgc cattgcccgc aactgctgcc agacggtaag cacgcgatcg gactggaagc
    14101 ccaaccgatt catcccactg ttcactttaa gataaatatc caacggtgct tttagccgcg
    14161 cattttgcag tgctttgagc tgccagttgc tgtgtacgca ggtggtcagg cggtgctggt
    14221 cataaatctc cagatcctga gcatggaaaa atccttccag catcaggatc ggccccttcc
    14281 agccgcgctc acgtaacgtt attgcctctt ccaggttaag taatgcaaag ccatcggtgg
    14341 ccccgagcgc gctccagata cgctcaatac catgcccgta agcgttcgct tttaccaccg
    14401 accagacgcg cgcgtgcggc gcggcctggc ggacaatgga cagattctgt tttaatgcct
    14461 gcagatcgag gctggcctgt atcggacggg tcatctcgtt tccttagctg tgtgcgccat
    14521 gtaaatggcc cggacgtgat ggcgtaaatc cacggctgta gcgcgctacg cttagatcct
    14581 catatgggat cgctggcgtg cgaccagaga gcagatcgct taacaactga ccggaaccgc
    14641 aagccatcgt ccagccgagc gtgccgtgac cggtattcag ccacagattt ttaaagcgtg
    14701 tactcccgac aaccggcgta ccgtctggcg tcatcgggcg caaacccgtc cagaaagtcg
    14761 cctgctcgac atgaccgccg cgtggataga gatcgcgaac caccatttcc agcgtttcac
    14821 gacgcggttg caacagctcg gtgttaaaac caacaatttc cgccattccg ccaacacgaa
    14881 tgcggttatc gaaacgggta atggcgattt tgtaggtttc atcaagaatg gtggatactg
    14941 gcgcaccatc ttcctgcgca attggaatag tcagcgagta gcctttcagc ggatacaccg
    15001 gaatatcaac aatgcctttg agcatcgccg tcgagtaaga accaaacgcc atcacatacg
    15061 catcggcctt aatcacttca tcgccaaact tcacgccgta gatttgctcg ccgtcgcaaa
    15121 gcagttggtc aacgggcgta ttaaagcgga atttaacccc cgcctgctct gccatccgcg
    15181 ccagattctg ggtaaacagc tggcagtctc cggtttcatc attgggtaac tgcaggccgc
    15241 ccgtcagttt gtgcgctact tctgccagcg cgggatccac ttccgccagg cggctggatt
    15301 ccagcagctg atacggtacg ccggcatctt ccagcacggc gatatcgcgg gtcgcatttt
    15361 catactgttg ttcggtacgg aacagttgca gcgtcccacc ctgacgccct tcatactgaa
    15421 tattggtttc ggcgcgcaat gctttcaggc aatcacggct gtattccgcc agacgcacca
    15481 tccgcccttt gttttccatg tagtggctgg tgtcgcagtt acgtaacatt tgccacatcc
    15541 atttcaactg aaactgcgta ccgtcgagac gaaccgccag cggcgcatgg cgctggaaca
    15601 tccatttaat cgcttttaaa ggcacacctg gtgctgccca cggtgcagca tatccggggg
    15661 agatttgccc ggcattcgcg gcactggttt ccagggcggc ccccggctcc cgatcaatga
    15721 cggtgacctc atgtcctgcc tgatttaagt accaggcgct ggcaacgcct accacaccac
    15781 ttcccagtat gacaactcgc atagccactc cgataacggt agatacagta aaagaataat
    15841 aatctaatta catcttgata actcagttga aaatattatt caacatacgg cttttttatg
    15901 gtgagctgac tcacatctcc ctgactctgc ggcaatatgg cgtctttagt atctcctgct
    15961 gaacgccctt tttatccagc ataaaattcc gttcagaagc ggattagtgg cactctgata
    16021 ataagaaatc gcaaagaaaa aaattctccg gcgacgcggt ttttaacagg tgttctatgc
    16081 ttgaaatgag gtgctccaca cagagagcgc cgacaacaat gaggtgcgcg tatggcgacg
    16141 atcgattcta tgaataagga caccacacgt ttgagcgatg gacccgactg gacgttcgac
    16201 ctgctggatg tttatctggc agagatagac cgggtggcga aactctaccg gctggatacc
    16261 tacccgcacc agattgaagt gataacctca gaacagatga tggatgccta ttccagcgtc
    16321 ggcatgccaa ttaactatcc gcactggtca ttcggtaaaa agtttatcga gactgaacgg
    16381 ctgtataagc acggtcagca agggctggcc tatgaaatcg tcattaactc taacccgtgt
    16441 atcgcttact tgatggaaga gaacaccatt actatgcagg cgctggtgat ggcacacgcc
    16501 tgctatggac ataactcttt ctttaaaaac aattgcttat tccgcagttg gaccgacgcc
    16561 agttcgattg tcgattatct gatttttgcc cgtaaatata ttaccgagtg cgaagaacgt
    16621 tatggcgttg atgaagtaga acggcttctg gactcgtgcc acgcgctgat gaactacggc
    16681 gtggaccggt acaaacgccc gcaaaaaatc tcgctgcaag aagagaaagc ccggcagaaa
    16741 agtcgcgaag agtatctaca aagtcaggtc aatatgctct ggcgtaccct gccgaagcgc
    16801 gaggaagaga aaacggttgc tgaagcgcgc cgctatccgt ccgaaccaca agaaaacctg
    16861 ctctatttta tggagaaaaa tgcgccactg ctggaatcat ggcagcgtga aatcctgcgt
    16921 attgtgcgta aggtgagcca gtatttttat ccgcaaaaac agactcaggt gatgaacgaa
    16981 ggctgtgcga ccttctggca ctataccatc cttaaccatc tgtatgatga agggaaagta
    17041 acggaacgtt ttatgctgga gtttttgcac agccacacca atgtggtctt ccagcccccc
    17101 tataacagcc cgtggtacag cggcatcaac ccgtatgccc tcgggttcgc catgttccag
    17161 gatattaaac ggatttgtca gtcgccaacg gaagaagaca aatactggtt cccggatatc
    17221 gccggttccg actggctgga aacgctgcat ttcgcgatgc gtgatttcaa agatgagagt
    17281 tttatcagcc agtttctgtc accgaaagtg atgcgtgatt tccgcttctt caccgtgctg
    17341 gatgatgatc ggcataatta tctggagatt tccgctattc ataatgaaga aggttatcgg
    17401 gagatccgta accggttatc gtcgcaatat aacttaagta atctggagcc gaatattcag
    17461 atctggaacg tggatttgcg cggcgaccgt tcgctgacgc tgcgttatat tccacataat
    17521 cgcgcaccgc tggatcgggg gcgcaaagaa gtcctgaagc atgtgcatcg cctgtgggga
    17581 tttgatgtga tgctcgaaca gcaaaacgaa gacggcagca tcgagttgct ggaacgttgc
    17641 ccgccaagaa tgggaaatct gtaaaaacaa caaaaaaccc ctcgtttgag ggggttgctc
    17701 tttaaacgga agggattatc gcccctgaat ggctaaatca cccggcagat ttttctgcat
    17761 ccggtgccaa atctcgccac tctcatgccc atagcgacgc actgtttcgt acacctgatc
    17821 gtgcgcgcct tcactgcaca acgccgacag tttgtggtag aagcccagcg ccagactgcg
    17881 cgcttccgga ttggcgaaat agtgacgacc aatgcgcgta tacagccctt tcatcccgtt
    17941 aaggatcaga ccgtaaatcg ggttgccgga agcaaacgcc aggccgcgga atatgttgta
    18001 atccagctcg gcaaaggcat cggcgtgatc ggccacttca ttagcggtag ccagcacttc
    18061 ctgcgcttta tcgggatgct gacgaaacgc ggtgcgaata aaaatagtgg aaatattggt
    18121 acgcaccgac agcaaattat caataagctg cggcacactt tcgtgatcca gtcgcgccag
    18181 tgtttcaagg atatttaaac cggaagtttc ccagaaatta ttcaccttcg tcggcttgcc
    18241 atgttgaatg gtcaaccagc catctcgtgc cagacgctgt aacacttcac gtaacgtagt
    18301 acgcgttacg ccaattaatt ctgaaagttc acgttctgcg ggcaaaatag tcccgggagg
    18361 gaagcggtta ttccagatac tttcaataat gtactcttcc gcgaaacccg ccgggctttg
    18421 cgccttaatg accatagtga gatttccata acacagcaaa acaaagttgg actcatcata
    18481 ccagagggct aactacgctg atagcagaca aaaagaagaa aaagggatca aggcttcatt
    18541 ttccgcatct ctttcccctt gactatcaag acttgcctca ctcctgcctg accgttaagc
    18601 tttgcgttta aacataaaaa catcattgat tatttgtaag gtaagggaaa ccatcatgga
    18661 gatctcctgg ggccgcgcgc tatggcgcaa ttttttgggc cagtcccccg actggtacaa
    18721 actcgccctc atcattttct taatcgtaaa cccgttaatt ttcctcatca gccctttcgt
    18781 cgcgggctgg ttgctggtag cggaatttat tttcactctg gcgatggccc tgaaatgcta
    18841 cccgctactc cccggtggtc tgttggctat cgaagcggta ttcatcggca tgaccagcgc
    18901 ggaacacgtc cgtgaagagg tggcggcaaa tcttgaagtc ttgctgttat tgatgtttat
    18961 ggtggcgggt atctatttta tgaaacagct gttgctgttc atatttaccc gtttgctgtt
    19021 aagcattcgc tccaaaatgc tgctgtcgct ctctttttgc gtggcggcag cgttcctctc
    19081 cgcgttcctc gatgccttaa ccgtcgtggc ggtggtgatc agcgttgcag tcggttttta
    19141 tggtatttat catcgcgtag cctcttcccg taccgaagac accgacctgc aagacgatag
    19201 tcatatcgac aagcattaca acgtggttct ggaacagttc cgtggctttc tgcgtagcct
    19261 gatgatgcat gccggtgtcg gcaccgcatt aggcggcgta atgaccatgg tgggcgaacc
    19321 acagaacctg atcatcgcta aagcggctgg ctggcatttt ggcgatttct tcctgcgcat
    19381 gtcgccggtg accgttccgg ttctgatttg tggcctgtta acctgcctgc tggtagagaa
    19441 gctgcgttgg tttggctacg gtgaaacgct gccggagaaa gtccgcgaag tgttgcaaca
    19501 gtttgacgat caaagccgcc accagcgtac ccgtcaggat aaaatccgtc tgattgtcca
    19561 ggcgattatt ggcgtctggc tggtgactgc gctggcgttg catctggcgg aagttggctt
    19621 gattggtttg tcagtcatta ttctggcaac atcattgacc ggtgtcaccg atgaacatgc
    19681 tatcggtaaa gccttcaccg aatctctgcc attcaccgca ctgttgacgg tgtttttctc
    19741 ggtagtcgcg gtgattatcg accaacaact gttttcgccg attattcagt ttgtgttgca
    19801 ggcatctgaa catgctcagc tgtcgctgtt ctatattttc aacggtctgc tgtcatccat
    19861 ttcggataac gtcttcgtgg ggacgattta tatcaacgaa gcgaaagcgg caatggaaag
    19921 tggcgctatc acgttgaagc aatacgagct gctggcggtc gccattaata ccggtaccaa
    19981 tctgccctct gtcgctacgc cgaacggtca ggctgcgttc ctgttcctgc tgacctctgc
    20041 actcgcgcca ttgattcgcc tctcttatgg ccgcatggtg tggatggccc tgccttacac
    20101 cctcgtactg acactcgtcg gcttgctctg cgtcgagttt acgcttgccc ctgtaaccga
    20161 atggtttatg caaatgggct ggatagcaac gctttgataa caacttaccg ggcatataaa
    20221 tgcccggttt gccttttcgc ccgataattg tccaattgcg tcctattttt gctgcctcct
    20281 ggtggcgcag cgaatgaatt ggtttaaact gcgcactcta tgcatattgc agggaaatga
    20341 ttatgttgcg atttttgaac caatgttcac aaggccgggg cgcgtggctg ttgatggcgt
    20401 ttactgctct ggcactggaa ctgacggcgc tgtggttcca gcatgtgatg ttactgaaac
    20461 cttgcgtgct ctgtatttat gaacgctgcg cgttattcgg cgttctgggt gctgcgctga
    20521 ttggcgcgat cgccccgaaa actccgctgc gttatgtagc gatggttatc tggttgtata
    20581 gtgcgttccg cggtgtgcag ttaacttacg agcacaccat gcttcagctc tatccttcgc
    20641 catttgccac ctgtgatttt atggttcgtt tcccggaatg gctgccgctg gataaatggg
    20701 tgccgcaagt gtttgtcgcc tctggcgatt gcgccgagcg tcagtgggat tttttaggta
    20761 tggaaatgcc gcagtggctg ctcggtattt ttatcgctta cctgattgtc gcggtgctgg
    20821 tggtgatttc ccagccgttt aaagcgaaaa aacgtgatct gttcggtcgc taatccattc
    20881 ggcgctcctg cgggagcgct tttttcctgc cgctatattt atttgacccg cagtaaatca
    20941 gaacttcgcg ttgtataacg tggtgaaagc atagctcgct tcatctgcca ttgttgttgg
    21001 atcccctgcc cggcaaaata gagtgttcct ctgccctctt tagcattcag agtatccatt
    21061 accgccatca attgctcact cccggggcgc ggtgcgttgt catcgaataa attgagctgc
    21121 gcgactccct gactgaagaa atcccccagc atcacgcccg ctttttggta gcgatggccc
    21181 gcttgccaga tggcatccag agatcgcgta gcagcgttaa tgatatccct gctgtcctga
    21241 gtgggcgtca gcagttttac cgacgcgcta ttgccgtaat aaggttcatt gagcgcaaat
    21301 ggtgacgtct taataaatgt ggagataaac cgacaatatt gatgctcact gcgaagtttt
    21361 tccgccgccc gggcagcgta actacaaatg gcctgccgca tcgacgtata atccgtgatg
    21421 cgttcaccaa acgagcggga acagataatt tcctgcttcg tcggtgcaaa ctcttccagt
    21481 tgcaaacagg gttcgccgcg cagttcacgc accgttcttt cgagcatgac attaaaatgt
    21541 ttacggataa accggatatc tgtatccgcc aaatcgagaa cggttttgat ccccatcgcg
    21601 tccagttttt tgctgatccg ccgtccaatc ccccagacgt catccacggg gagagcagac
    21661 attaatttac gctggcgttc cagatttgat aaatccacca ccccacccgt ctgccgctgc
    21721 catttttttg ccgcatgatt ggcaagctta gccaacgttt tggtctgggc gatccccaca
    21781 ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa tttctctgcc aaaatcagtc
    21841 agatcgcgac aattacgcac acctgtcaga tcgcagaatg cttcatcaat actgtaaatc
    21901 tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc gattgctcat gtctgcgtaa
    21961 agctcataat tgctgctaaa gcaaaccaca ccacagcgac gaaacagatc tttttgtttg
    22021 aaccagggat cgcccatttt aacgcccagc gcctttgcct cagcgtttcg ggcgataacg
    22081 caaccgtcat tattcgatag cacaaccacc ggtttacccc ataaatcagg gcgaaacacc
    22141 gtctcacagc tggcataaaa cgcgtttaca tcacagaggg caaacatcag cgcatcgcct
    22201 taacgacgtg gatcaccaca ccaaagacat ccagcgtatc ttcgctactg atggtaatgg
    22261 gcgagtacgc actgttcatg ggaataagct gtaccgtcgg gcgtagttgc aattttttca
    22321 ccgtaaactc gccgtcaaca gcagcgatga caatatcacc atggctggcg gtaatagcac
    22381 tatcgacaat cagtaaatca ccgtcactaa ttccaccatc aatcatagaa tcaccacttg
    22441 ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag ttgattcaga tcgatgcgct
    22501 gttcaacgta atctgctgcc ggtgaaggaa agccacactg aacaagatcg ctaaatagcg
    22561 gaaaagtcac aatttcgcgg agatccgcag gcttgataaa caacataata atctgcctga
    22621 agttatactg tttttatata cagtggtctg ttcttgccag cagatcaata ctgattcagg
    22681 ctatcaatat ttgtcgctgc atagactgct gatttttcgt tctcttatcc tgtgctcacg
    22741 tggccttctg gcgacgacgc tcatccagca gaaatgaaaa atatcacccg gcaaaaaaat
    22801 agaatagaag cattcgccat aatgacatta aacattg
//
LOCUS       DAZOZX010000056        22446 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZOZX010000056 DAZOZX010000000
VERSION     DAZOZX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.056"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            82..2682
                     /locus_tag="ACHUPT_002156"
     CDS             82..2682
                     /locus_tag="ACHUPT_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR0819724.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHUPT_002157"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHUPT_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHUPT_002158"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHUPT_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHUPT_002159"
     CDS             4302..4817
                     /locus_tag="ACHUPT_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0819725.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHUPT_002160"
     CDS             4783..5520
                     /locus_tag="ACHUPT_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0819726.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHUPT_002161"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHUPT_002161"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0819727.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHUPT_002162"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHUPT_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0819728.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHUPT_002163"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHUPT_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0819729.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHUPT_002164"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHUPT_002164"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0819730.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHUPT_002165"
     CDS             10827..12734
                     /locus_tag="ACHUPT_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0819731.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHUPT_002166"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHUPT_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0819732.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHUPT_002167"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHUPT_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0819733.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHUPT_002168"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHUPT_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0819734.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHUPT_002169"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHUPT_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0819735.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHUPT_002170"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHUPT_002170"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0819736.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHUPT_002171"
     CDS             complement(17402..19162)
                     /locus_tag="ACHUPT_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0819737.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHUPT_002172"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHUPT_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0819738.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHUPT_002173"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHUPT_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0819739.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHUPT_002174"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHUPT_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0819740.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..22415
                     /gene="sxy"
                     /locus_tag="ACHUPT_002175"
                     /pseudo
     CDS             22005..22415
                     /gene="sxy"
                     /locus_tag="ACHUPT_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5730 a         5270 c         5881 g         5565 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tgggtagact ggccccctga atctccagac
    22441 aaccag
//
LOCUS       DAZOZX010000057        22433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZOZX010000057 DAZOZX010000000
VERSION     DAZOZX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.057"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="ACHUPT_002176"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="ACHUPT_002176"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0819741.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            691..1317
                     /locus_tag="ACHUPT_002177"
     CDS             691..1317
                     /locus_tag="ACHUPT_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0819742.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1338..3439
                     /locus_tag="ACHUPT_002178"
                     /pseudo
     CDS             1338..3439
                     /locus_tag="ACHUPT_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3452..4090
                     /locus_tag="ACHUPT_002179"
     CDS             3452..4090
                     /locus_tag="ACHUPT_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0819743.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4154..4822
                     /locus_tag="ACHUPT_002180"
     CDS             4154..4822
                     /locus_tag="ACHUPT_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0819744.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4819..5604
                     /gene="phsC"
                     /locus_tag="ACHUPT_002181"
     CDS             4819..5604
                     /gene="phsC"
                     /locus_tag="ACHUPT_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0819745.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5608..6420
                     /gene="ydhT"
                     /locus_tag="ACHUPT_002182"
     CDS             5608..6420
                     /gene="ydhT"
                     /locus_tag="ACHUPT_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0819746.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6432..7854)
                     /locus_tag="ACHUPT_002183"
                     /pseudo
     CDS             complement(6432..7854)
                     /locus_tag="ACHUPT_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7980..8285)
                     /locus_tag="ACHUPT_002184"
     CDS             complement(7980..8285)
                     /locus_tag="ACHUPT_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0819747.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8393..8469)
                     /locus_tag="ACHUPT_002185"
     tRNA            complement(8393..8469)
                     /locus_tag="ACHUPT_002185"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8433..8435),aa:Val,seq:gac)
     gene            complement(8474..8550)
                     /locus_tag="ACHUPT_002186"
     tRNA            complement(8474..8550)
                     /locus_tag="ACHUPT_002186"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8514..8516),aa:Val,seq:gac)
     gene            8858..10114
                     /locus_tag="ACHUPT_002187"
     CDS             8858..10114
                     /locus_tag="ACHUPT_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0819748.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10155..11528)
                     /gene="mdtK"
                     /locus_tag="ACHUPT_002188"
     CDS             complement(10155..11528)
                     /gene="mdtK"
                     /locus_tag="ACHUPT_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0819749.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11743..12384
                     /gene="ribE"
                     /locus_tag="ACHUPT_002189"
     CDS             11743..12384
                     /gene="ribE"
                     /locus_tag="ACHUPT_002189"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0819750.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12424..13572)
                     /gene="cfa"
                     /locus_tag="ACHUPT_002190"
     CDS             complement(12424..13572)
                     /gene="cfa"
                     /locus_tag="ACHUPT_002190"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0819751.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13863..15074)
                     /gene="punC"
                     /locus_tag="ACHUPT_002191"
     CDS             complement(13863..15074)
                     /gene="punC"
                     /locus_tag="ACHUPT_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0819752.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15187..16119
                     /gene="punR"
                     /locus_tag="ACHUPT_002192"
     CDS             15187..16119
                     /gene="punR"
                     /locus_tag="ACHUPT_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0819753.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(16116..17141)
                     /gene="purR"
                     /locus_tag="ACHUPT_002193"
     CDS             complement(16116..17141)
                     /gene="purR"
                     /locus_tag="ACHUPT_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0819754.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17440..17529
                     /gene="cydH"
                     /locus_tag="ACHUPT_002194"
                     /gene_synonym="ynhF"
     CDS             17440..17529
                     /gene="cydH"
                     /locus_tag="ACHUPT_002194"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0819755.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17695..18864
                     /locus_tag="ACHUPT_002195"
     CDS             17695..18864
                     /locus_tag="ACHUPT_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0819756.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19010..19591)
                     /gene="sodB"
                     /locus_tag="ACHUPT_002196"
     CDS             complement(19010..19591)
                     /gene="sodB"
                     /locus_tag="ACHUPT_002196"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0819757.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19719..20546)
                     /gene="mepH"
                     /locus_tag="ACHUPT_002197"
     CDS             complement(19719..20546)
                     /gene="mepH"
                     /locus_tag="ACHUPT_002197"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0819758.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20881..21228
                     /gene="grxD"
                     /locus_tag="ACHUPT_002198"
     CDS             20881..21228
                     /gene="grxD"
                     /locus_tag="ACHUPT_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0819759.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21342..21752)
                     /gene="tnpA"
                     /locus_tag="ACHUPT_002199"
                     /pseudo
     CDS             complement(21342..21752)
                     /gene="tnpA"
                     /locus_tag="ACHUPT_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            21775..>22433
                     /locus_tag="ACHUPT_002200"
     CDS             21775..>22433
                     /locus_tag="ACHUPT_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0819760.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         5813 a         5215 c         5808 g         5597 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga aattatacga atttcggtat gtattttaat ttatagcgat
      361 aatgttattt ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta
      421 tttcaaatga taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat
      481 aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat
      541 tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt
      601 tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag
      661 cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat
      721 attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca
      781 attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca
      841 gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt
      901 tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc
      961 tcccgtatca aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc
     1021 ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa
     1081 tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc
     1141 gtcgatcata aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg
     1201 gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca
     1261 aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc
     1321 ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca
     1381 cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg
     1441 gcttcggcta caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag
     1501 cgaataaatt agtctttgct accggcccat tacctggatc tggtgccccc tgtagttctc
     1561 gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat
     1621 gggtggcttt tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg
     1681 gaaggcgaaa tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc
     1741 cgatttctta tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag
     1801 cccggaaacc tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg
     1861 catgttgaat agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa
     1921 aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga
     1981 gatgaagcgt ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt
     2041 cgtgccaagt acgccacaat cgtgggcaga gtattcagat cccaagtcac gctggacagc
     2101 acgtaaaggg ctgttttggg gcgcggctga aggtggtccg attgaaacgg gtgaaattcc
     2161 gccaggcaat cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggacc
     2221 ggcggcagag aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat
     2281 gacccaaatg aatattcctc gggtgaaaga gtttggcgtg cccagcacag gtggtaacac
     2341 ttgtgtagca aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga
     2401 taaagacgat ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg
     2461 cctatggtgt aactacgggc agttgcatcg cgactttact tattgttaca gcaaaggtgt
     2521 gttcaagcgt gttctgccag ctgaagagta tgcagaaatt cgctgggatc aactggaagc
     2581 gggtgacgtt aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct
     2641 gagtcacctg gctgatggtt catatgccat cgcagaacgc tggaatttgg gtaaagagta
     2701 ctggggctac gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa
     2761 tgaagcgtca gcgcaggtcg gttccattgt taagtgtatg ttcaaccgtg actgcatgac
     2821 gcatacccat atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc
     2881 gaaagaactt tttggttctg aagatgctta cgatgaaacc aaaaactaca cgccaatcaa
     2941 tgacgcaaaa attaaatatg caaagtggtc gctgttgcgg gtctgtttgc ataacgccgt
     3001 cactctgtgc aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg
     3061 gggcgatctg gcgcttgaag ccaaattctt caaagcgatc accggcgaag agatgactca
     3121 ggaaaaatta gatttagctg cagagcgtat ctttacgttg catcgtgcct acacggtaaa
     3181 accgatgcaa accaaagata tgcgtaatga acacgatctt atctgttcct gggttttcga
     3241 caaggatccg cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat
     3301 gcatgcttcg ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgtcc
     3361 aacccgcgaa acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca
     3421 caatctactg cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc
     3481 gaaagtcagc gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt
     3541 tgatacggta accgaaccag aacctgctga aatcctggct gagttcattc gccagcattc
     3601 tgcggctggg cagctggtgg cgcgcaccgt gtttctctcg ccaccctatt cggttgcaga
     3661 agaggaactg tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc
     3721 ttgtatgacg ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta
     3781 cgcggcgatg tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt
     3841 tcgttttgag tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc
     3901 atattacttc caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga
     3961 atatgccgat atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg
     4021 atttatgacc tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca
     4081 gaacccctga aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt
     4141 aggggataat cagatgtcct tcactcgacg caaatttgtt ctggggatgg gaacagtaat
     4201 cttttttact ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg
     4261 gtatgccatg attcatgacg agtcacggtg caatggctgt aatatttgcg cccgcgcatg
     4321 ccgtaaaact aaccacgtcc ccgctcaggg aagccgttta tcgatagcac acattccagt
     4381 caccgataac gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga
     4441 agatgctccg tgcatcgacg tatgcccgac gggggcatcg tggcgtgatg agcaggggat
     4501 cgtgcgggta gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta
     4561 cctggttcgc taccttaacc ctgtcactaa agtggcagat aaatgtgatt tctgcgctga
     4621 gtcccgactg gcaaaaggct ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt
     4681 gatctttggt cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta
     4741 tcaatatcag ctacctggtg ccggaaaacc gcatctgtat cgccggttcg gtcaacattt
     4801 gattaaaaag gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta
     4861 gcgaactatg tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga
     4921 gtattgctgg ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc
     4981 gtgaagaaat cagccaccga tcatggtgag aaggtttatc tttacagcaa agcggtcaga
     5041 ttatggcact ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat
     5101 cactttgcga tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc
     5161 ggatttttgt tactggcatg ctggctcggc tttgtgctga tcaatgccgt tggggataat
     5221 ggtcaccact atcgcattcg tcgtcagggg tggctggaac gagcggcaaa acaaacgcga
     5281 ttttatttgt ttggcattat gcagggggaa gaacatcctt tcccggcaac aacacagtct
     5341 aaatttaatc ccttacagca ggtcgcctat gttggtgtca tgtatggatt gctgccgttg
     5401 ttactattga cggggctgtt gtgtctctat ccgcaagccg tgggagatgt gtttcctggc
     5461 gtaagatact ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc
     5521 ttcggtcatc tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg
     5581 gtcgatggct atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg
     5641 cgtatcggag cggtgatgta tcgctggttc ctgcgccatt tcccacgcgg cggtagttat
     5701 gccgatattc gtcatgcact gattgaggaa ggatatacgg actgggcgga gagtctggtt
     5761 gagtatgcgt ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct
     5821 atgcaaaaac tggccacaga ccccggagag ataccatttt gcagtcagtt tgcccgcagc
     5881 gacgatcatg ctcgcatcgg ctgttgtgag gataacgccc gaatcgcaac cgctgggtat
     5941 gccgcgcaaa ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt
     6001 cacattggca gctccggtga acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata
     6061 agcagcgctg gagatagtag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca
     6121 ttgggtgaac ggtgtcatgt cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc
     6181 gcaaacgcca gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat
     6241 tcaacggtag tgagcacggg cgtggtcgac tcaattattc tcgggctagg gggaagcgcc
     6301 gcactggcat atcatgacgg cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac
     6361 aatattcgaa ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga
     6421 tagctttacc tttaatcaaa cgaaagacgc cgacgagcac gggatgcagg ctttacgacc
     6481 gcccgagcca tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg
     6541 tcgtgcatca accagggcaa cgcgccgaac gctacgcgtc cacgaatatc ttcgggttgc
     6601 tgtaacgtat aatcttcgcc aacatcaggg atttcagatc cccgcttcac gtaaggccag
     6661 tagtcgacga atagactctg aagggattgc cgcataatta tcgatgaaaa ccctggcaag
     6721 gcctttactg aaccgtttat ggtcgtgttc attcagatga ggaacaattt cctgtacggc
     6781 ttcatgcagg cgcagaatga cataacgcca gggaacagta tgtttttctc gtttattgcg
     6841 ttcgacttcc tggagatttt tttctgccca gtcaaatggg tcgcgttgtt tgcgctcggc
     6901 aaaccaggct tgagcaaagg aatcgacatt caggctctct aaggctatgc gttggctcca
     6961 gtctggatca gcaaacttga tttcctctac tatcaatcta aatacccgat ccaaaaggcc
     7021 ctcttcacct ttttgaatct cagcatttaa tgcctgatcg gtgacgatgt gtaagggctc
     7081 gtagggaata gggcaataga aatcggcttc gggtaaaata cccgtgcgcg acatcaacgt
     7141 aatatttagc ttttcacttg cgttatcgcg gtgaaaaacg acgtgttgtt tatcatcttc
     7201 aatgaacgaa ccatgctgaa tagccactgc cattgccgca tccagtccgc tcaaggatgt
     7261 tcccataata cccacgttac acgcatcgac ctttgcttcc atcaggcctg accacgggct
     7321 gggaaaatac gttctggttg cttcttcttc atcaggccag acgtgacccg tggcaatcac
     7381 cgctaaatca aacgtctcgc tgggtaaatc ctgatttgta gcgagcatga cgccagcatt
     7441 tgtaatttgc agatcggtaa cctggcatga ttcataaaca gccactgcaa atttttgctg
     7501 tcgtgcctgg tctactaatc gtaaaaattg atcgcggaaa tattcgccca gcagaattcg
     7561 cggtaaaaac tgacgatcgt gcaaggtttc ttttttaacg ccataacgct ggagatggct
     7621 ggcttcttgc ttttgtagcc attcgagata cgtacaatta atcggcggta tttcaatact
     7681 ggcaatattt gccagcatca tttttgagtt ttcctcatca ctgtatggca tcccgacacc
     7741 ggcctcgtca gcctgctcga agatagaaat agaaagtgga gtttgttgct gtagaagcga
     7801 gaataaggtg tagatccccg taggcccggc acccacaata gcaatttttt tcattgttac
     7861 ttacctgtac gcattctttc gaatttagtg ttatcagata agtttggttg agatttttag
     7921 aatttgagaa atttcagaca gggatgaaaa gggcgctaac cgcccttgat taaattctgt
     7981 caggcgagtt ttgcctgatt gatttgacta agtggctcat tgacatcgaa aactttggcg
     8041 acaacttcct caacgccgag atttttcagt cgggcagtat gtttttccag ataggcaagg
     8101 gcgctttttt catcagtgaa aaggtagatc ccaccggctt cgtggttctt ttcactttct
     8161 gtccatacct tccacagaaa accaggttcc tgattaatcg actcagcaag tggtttaagc
     8221 tgctcagcca ttgcgtcgcc aaatgggcca ttaaaagcaa aatgaagttg taacaaagtt
     8281 gccatagcga ttctccttct gataacgcat acgaagacag tgtaaatctt ttaacgtatt
     8341 ggatacagag aagaaacaga gggaaaaagg aaaagaccag attaagaaaa tctggtgcgt
     8401 ccgagtggac tcgaaccacc gacccccacc atgtcaaggt ggtgctctaa ccaactgagc
     8461 tacggacgca ggatggtgcg ttcaattgga ctcgaaccaa cgacccccac catgtcaagg
     8521 tggtgctcta accaactgag ctatgaacgc aacgttgtag gtgacaacgg ggacgaatat
     8581 tagcggcaga gtggaaaggt ggcaagaggc aaaacgtaat tttctgcgct atttcgaccg
     8641 tttgcagagc ttttaagcaa attggctata ttttgttgat ttgcaagggc gatttttatt
     8701 caggatcgca tttacatctg atacaaccca ataattcgtg tatcagagtt taaacagagt
     8761 tgtttgccac catctcaaat ctgtcgtgga taaatcctcc gtagccttta cacttaggta
     8821 gcaataaagg cacacagatg cagcaaagga ataaatcatg ggatctgatg cgaaaaactt
     8881 gatgagcgac gggaatgtgc aaattgttaa gaccggcgag gtccttggcg cgacgcaact
     8941 tactgaaggc gagttgattg ttgaagctgg cggaagagcc gaaaataccg tggtcacggg
     9001 ggctggctgg ttgaaagtgg caaccggtgg gatcgccaaa tgcacacagt acggtaacaa
     9061 tggcacgcta tcggtcagcg acggtgccat tgccacagat attgttcagt ccgagggagg
     9121 cgcaattagt ctctctacgc tcgctacggt taatggccgc catcccgaag gtgaattcag
     9181 cgttgatcag ggttatgcct gcggtttgtt gctggaaaat ggcggtaacc tgcgtgtact
     9241 ggaaggacat cgcgcggaaa aaatcattct cgatcaagag ggcggcctgt tggttaatgg
     9301 gacaacctca gcggtcgtgg tagatgaagg tggtgaattg ttggtgtatc caagtggaga
     9361 agccagcaat tgtgagatta atcagggcgg cgtttttatg ctggccggga aagccagtga
     9421 tacgttgctt gctggtggca ccatgaataa tctcggtggt gaagactctg acactattgt
     9481 tgagaatgga tccatttatc gtctggggac ggatggcctt cagctttaca gttccggtaa
     9541 gacgcaaaac ctgtcggtga atgtgggggg gcgggctgaa gtgcatgccg gtacgctgga
     9601 aaatgcggta atacaaggtg gaacagtgat cctgttgtca cccaccagcg cggacgaaaa
     9661 ttttgtcgta gaggaagatc gcgcaccggt tgaactgacc gggagtgttg cattactgga
     9721 cggcgcttca atgattattg gttatggcgc agagctgcaa caatcaacga ttactgtaca
     9781 gcagggcggt gtgttgattc tcgacggcag tacggtaaaa ggtgacagcg tcactttcat
     9841 tgttggtaac atcaatctga atggcggaaa actgtggctg atcactggtg cggcaacgca
     9901 tgtgcaactg aaagtgaaac gcctgcgcgg agagggagcg atttgcctgc aaaccagtgc
     9961 gaaagaaatt tcacctgact tcatcaatgt gaaaggggaa gttaacgggg atatacgcgt
    10021 tgagataaca gatgccagtc ggcaaactct gtgtaacgct ctgaaattac agccagacga
    10081 agacgggatt ggcgcaacgc tccagcctgc gtaaaaacac aggcgcaacc cgtgaaggtt
    10141 gcgccttgtc tttattagcg ggacgctcgt tgcagaatga tggctgacgg catacgttgc
    10201 aggaaccgca tacgcagcat catcataatg gctgccgatg tcaggccgat aataaagcct
    10261 atccagaagc ctgctggccc cataggttca acgaccagat cggtcagtgc cagaatatag
    10321 ccgcttggca agcccagcac ccagtaagct gtaaaggtga tatagaaaat ggaacgcgta
    10381 tctttataac cacgcaaaat cccactgcca atcacctgga ttgagtcaga aatctgatat
    10441 accgccgcca gcaacatcaa atgcgcagcc agcgttacaa cctcgggatt gtcgttgtac
    10501 aacagggcga tttgctcccg cagtgaaacc gtgaaaatgg ccgtcagggt tgccatacag
    10561 acacccacca taagcccggt ccgcgcagcg gtttgcgcat ccagcgttga gccctgaccc
    10621 agacgataac ctacgcggat agttaccgct gccgccagcg acattggaag cacaaacatc
    10681 agtgaactaa agttcagggc aatctggtgt cctgcgacat caacaatacc gagcggagac
    10741 actaacagag ccacgacggc aaacagtgtc acttcaaaga acagtgccag cgcaatcggc
    10801 aaaccgagtt gaatcagtcg tttcataacc gcaggctcgg gcttagcggt gcctttttcg
    10861 ttacgaatat cgcgcatgga gcgggcgcgt ttaatgtaag aaaccatggc aaggaacatg
    10921 acccaataca ccgccgcagt agccacgcca caaccaacgc caccgagctc aggcataccg
    10981 aaatgaccat aaataaagat atagttcacc gggatgttca ccagcaggcc gataaagccc
    11041 attaccatac ccggcttggt ttttgccaga ccttcacatt ggttacgggc aacctggaag
    11101 aacagatatc ccggcgcgcc ccacaacaac gcacgcagat aacccacggc tttgtccgcc
    11161 agagccggat cgatgttttc catgtagcgg ataatgtaac ctgcattcca cagcaccagc
    11221 ataatgagaa cggaaacaaa acctgccagc cagaaacctt gtcgcacctg atgcgcaatg
    11281 cgctcacgtc gaccggaacc atttaattgc gcgataaccg gcgttaatgc cagcagcagt
    11341 ccgtgaccaa agaggatcgc cggaagccag atagaagtac cgatagcgac cgccgccatg
    11401 tcggtggcac tatagccgcc cgccatcacg gtatcgacaa aacccatcgc agtttgggcg
    11461 atttgcgcga gaatcaccgg gattgctaat gctaataaca gacgcgcttc actgatatac
    11521 ttctgcacgt gaacaccttt tatttgtagt tatatgagag actaaaaagc cgcctgaatg
    11581 ggcagcaaaa agaagaagca ggggaaattc agtctattgt agcgaggatt tattaattct
    11641 ccagtgaaaa aatagccatt cttacccctt cactggtaag ggcgttttcc acctgctatt
    11701 gtgctgaaca gaatgtctta actgatttca ggagttgtaa gtatgtttac ggggattgta
    11761 cagggcaccg caaaactggt gtcgattgac gagaaaccaa attttcgtac gcatgtggtg
    11821 gagttacccg accacatgct ggacggcctg gaaaccggtg cttccgtggc gcataacggt
    11881 tgctgcctga ccgtgacgga aattaacggc aaccatgtca gttttgacct gatgaaagaa
    11941 acgttacgca ttaccaatct tggcgattta aaagtggggg attgggtaaa cgttgagcgt
    12001 gcggcgaaat tcagtgatga aattggcgga cacttaatgt caggtcatat tatgaccacc
    12061 gctgaagtgg cgaaaatatt aacctcggaa aataatcgcc agatctggtt taaagtccag
    12121 gatagtcagt tgatgaaata tatcctgtac aaaggattta ttggcatcga cggtattagc
    12181 ctgaccgtcg gcgaagtcac gccaacgcgt ttttgcgtcc atttaattcc ggaaacactg
    12241 gaacgcacga ctcttgggaa gaaaaaactt ggcgcacgcg tcaacattga aatcgatcca
    12301 caaactcagg cagtggtaga tacggtagaa cgtgtgctgg cggcacgaga aaatgccatg
    12361 aatcaaccag gtacagaagc ctgatctgaa ctcgcccccg aagagggggc gatagaatag
    12421 cctttagcga gcgactcgaa ggccgttttc aacaccgcgt gagaacacca cctgccagag
    12481 ctgaatatca cgggcgcgga aagcacctgc gcaggcattc agataatagg taaacatccg
    12541 tttaaagcgt tcactgtagt tatccgcaat ttctggccat gcggcgagga atcgttcata
    12601 ccacgccatc aacgtagtat cgtaatcagc accgaagtta tgccagtctt ccatcacaaa
    12661 gtggggttcg ctggattgag caatctggcg tacagagggc aggcaaccgt tcggaaaaat
    12721 atatttatta atccagggat caacattcag atcggttttt ttcgaaccga tagtatggag
    12781 caggaatata ccttccggtt tcaaattacg atccaccacc gcaaaatagg tatcgtaatt
    12841 tttcggtccg acgtgctcga acatccccac agaaacaata cgatcaaact ggtcgttcag
    12901 gtcacgataa tcttgcagca aaatggtgac atccaggcct ttacagcgtt cctgagccat
    12961 tttttgctgt tcggcagaaa tggtgacgcc gaccacgctt acgtcatagt tagatgccat
    13021 gtagtgcgct agtccgcccc agccgcagcc aatatccagt acgcgcatcc ctggttttaa
    13081 ctgcaatttt tcacaaatca ttttgagctt cgcctgctgg gcagattcca gattatcggc
    13141 atctttccag taagcgcagg aatattgcat gaagggatca agcatgcggc tgaacaagtc
    13201 attacccaaa tcgtaatgct ccttgccgac tatccaggca cgttttttac tctgcagatt
    13261 gaagagacga gcgccggcaa tacgcagcgt gtctttgaaa tgatggggga gttggttctc
    13321 gagacctgcg cgtaagactt tgctaaaaaa catatccagt cggtcacatt cccaccagcc
    13381 atccatataa ctttcgccta accccaaaga gccttcctgc agaacgcgtt taaaaaaatc
    13441 ggggtttttc acacgaatat ccgccggggc agaaccgtta atggctatac cggcacggct
    13501 aagtaattcg ttggcgatac ggtaccagtt gtcatccggt acactgactt cttctataca
    13561 cgatgaactc atagtttctc catcactggt cggtgatcag aaccttaaaa cagcgtagac
    13621 gctttttggg ctttgtgaga aatctcgcag aaaaaaccgc gagtctataa tccgacgtag
    13681 aacagaggga aaggaggaac ccttgccgaa atgaccatcc atagtgagtc gggaacgatc
    13741 ctgtccccgt aaaaacataa tatttatcgt agctttaaca actcgaaatt agtataggcc
    13801 gctaatttca atcatacaac aaaattttgt tagcagccta agtataagta tatcgatata
    13861 gatcagtgtg attcgctatg agcgacttcg gcattgccat gattcgggca gtcaacttct
    13921 tcacaacgtt gcatcatgta acccagcgcg accagcacta ctgttgataa catcacgctg
    13981 gtggtggtga gcaatggcgt gctgatacta ataagccagg aaactaccag acttgcgagg
    14041 aagcacagac ccagttgcag agtgttctgc aacgctgcgg cgcgaccggt tgcatgtggg
    14101 aagggacgca gcgcctgggc gacaacaata gggtagatcg cgccattggc aatcgccatc
    14161 acacagaatg ggatcaggat ttcgaccagc gacacatggc taatgaagcc cgcagcccag
    14221 gtcgcaatga cgctgacagc aaacagcacc agcaaccacg gtaataactg cttgccttgc
    14281 catttctgca gtgcggcgcg acagccataa ccaccaatca gaaacgcgat agtttgcggg
    14341 acataactta aaccaataac tgccgggctg tagcccattt cactaaggat gaacggtgaa
    14401 ccggtcagcc atgcaaaaaa actggctgaa caggctgcgt atatcagcac gttgccgcga
    14461 taggttttag aacgtagcag gtcggtaaag gtcagaccat cctgactatt gttacgggcc
    14521 ttcgtcgtgg gtttgagcca gaaaataggc agaatcagca ccacggtaat ggcaaacagg
    14581 gtggcgaaaa tcgcctgcca ggaaaaatgg accagcagcc agcttcctaa caggggagcc
    14641 agtgccggag acagacccac cagcggcatg atggtcgcaa aaatacggtt gactttctgt
    14701 gaaggataat aatctgtcac taatgcttgc cagataaccg ccgcggcgca gacccccaca
    14761 gcctgtacaa aacgcaatac cagcagcgtg gcggcgtttt ctacccacag catccccaga
    14821 ctacctaacg caaaaattgt caggccgatt aataacaccg gtttacgacc ataacggtcg
    14881 gagagtggcc cccacagaag ctgggctgcg gcaaaaccgg caaggaacag actaaggctg
    14941 gcactgacag cagacgcagg cgtttgcagg tcggcctgta tggcggcgaa agcaggcaga
    15001 tacatatcgg ttgccagaaa accgagtacg ctcaaacccg ccagccagac taaaaatctt
    15061 ttcccaggtt gcatgtatat tctctaaatc tctcaattaa acaccgccgc aaagtgtagg
    15121 gagtgaaatt agtgttgtga aacggtaata tttgatgact ggtttcaaaa attttgcagg
    15181 cagactatgt ggtcagaata ttcactcgaa gttgttgatg cggtagcgcg taatggtagt
    15241 tttagcgctg cggcacagga gctgcatcgt gttccttctg cggtcagcta taccgtgcgt
    15301 cagctggaag agtggctggc ggtaccgctc tttgaacgac ggcaccgtga tgtggagctg
    15361 accgctgctg gagcgtggtt tctcaaagaa gggcgctctg ttgtcaaaaa aatgcagatc
    15421 acccgccagc aatgtcagca gatagcgaac ggctggcgcg gtcagttagc tatcgcagtg
    15481 gataatattg tcaggccaga acgtacacgg cagatgatcg ttgattttta tcgccatttt
    15541 gatgatgtcg aacttcttgt ctttcaggaa gtgttcaacg gtgtctggga tgcgctttcc
    15601 gacgggcgcg tggaactggc aattggcgca acacgcgcga ttccggtagg cggtcgttat
    15661 gccttccggg atatggggat gctaagctgg agttgcgttg ttgccagtca ccacccgctg
    15721 gcgttgatgg atggcccgtt cagcgatgat acgttgcgca actggccgtc gttggtgcgc
    15781 gaagacacct cgcgaacgct acctaaacgt attacctggt tgctggataa ccaaaaaaga
    15841 cttgtcgtac ctgactggga atcatcggca acctgtatct cggcaggatt atgcataggg
    15901 atggtgccaa cacatttcgc caaaccgtgg cttaatgagg ggaagtgggt agcacttgaa
    15961 ctggaaaacc ccttcccgga ttcggcatgt tgtctgacat ggcagcaaaa tgatatgtcg
    16021 cctgcgttaa cctggctgct tgagtatctc agcgatagtg agacgctgaa taaggagtgg
    16081 ctgcgggagc cggaagagac tcccgcaacg ggtgattaac gacgatagtc gcggaacggg
    16141 ccgtcagcca cggagcggcg ttcaatcaaa cgcggatgca cttcaatgga ctgcggttct
    16201 tcacgtttgt tgacgatacg atccaacagc atgttgaacg ctgtttcacc cagcgaatct
    16261 tttggctgat ggatcgtggt cagcgccggc gtaaaatagc gcgcgttgcg cacgttatca
    16321 taaccgatca gcgaaacatc ctgcgggacg cgcaggccca tttcatcagc agcacaaagt
    16381 gcgcccattg ccatgatatc gccaccacag aagacggcag tagggcgatg cggctgcgac
    16441 aggatttgct gcatggcgcg ataaccggat tcaggttcaa agtcaccctg cacaatccag
    16501 ctttccggca ccttgatcat cgcttcttcc atcgccttca taaaaccggc aaggcggcct
    16561 gcgccggtgt tacgttccag cgggccgggg atgacgccga tttcgcggtg accgcgttca
    16621 atcagataac gcccggccat gtagccgcct tcgaacgcgt tatcaatgac cgcatcggtg
    16681 aagtcagctt ttgcttcacc ccagtccatc accaccattg ggatatggcg atactcttcc
    16741 agcatcgcca gcaacggctc tgggtactca gaacacatca ccagcagacc atcgacgcgt
    16801 ttttgcgcca tcatcgacag ataagcccgc tgtttctcaa gattgttcca cgcattaccc
    16861 agaatcaggg tgtaaccttt ctggaagcaa tttttttcaa ctgcttcaat gatctcggca
    16921 aaataggccg cttcgctgct ggtcgccagc aaaccgatag acttggtgtg gttaaccttc
    16981 aggctacgcg ccaccgcgct aggggagtag tgtaattctt taatcgctgc ccacacggcg
    17041 ttgcgcgttt cttcagcgac gaaacgtgtt ttgttgatca cgtgtgacac agttgtagtg
    17101 gaaacgtttg ctcgtttcgc tacatctttt attgttgcca ttccatttca ctccagaccc
    17161 taacttcagc tcctgcaaaa tcgcaaggta aacgtttgcc tttacacacc ttacgcacaa
    17221 caaaagtgtt gcggtacgcc ggaaaaacga cacggagcgt caggaagggg tcaatggcca
    17281 gtacgcaaat agtgtaagcg tggaattttg gccgatcttg acgaaaaggg gaagtggtaa
    17341 aaacacttat cattataaat gacgggagat tttttcatca cagtgtgtaa aaatgcgtaa
    17401 tatcactttt tgtgtggtaa taaaaaagga gaaaacttga tgagcaccga ccttaaattt
    17461 tcactggtaa caacggttat cgtcctcggt ttgatcgtag ccgtgggttt gactgccgcg
    17521 ctgcactgat ttcctctcga gggagcgcgt tctccctcgc atctccttcc tgtttgttag
    17581 caaaatggca aaagtgtttt ctctttttgt tattttcatt tttttgtgat tgctgtcatg
    17641 ctttcgccac tttaatgaat atcgcgtaat gacgcgaaga ggtggagata aggcatgaaa
    17701 attaactatc cgttgctggc gctggcgatt ggcgcgtttg gtatcgggac aacggagttc
    17761 tcgccaatgg gcttgttgcc cgtcattgcg cgcggtgtgg atgtctcgat tcccgctgcc
    17821 ggaatgttaa tcagtgccta tgcagttggc gtaatggtgg gcgcgccgct gatgacgctt
    17881 ctactttctc atcgtgcccg ccgcagtgcg ttgattttcc tgatggcaat tttcacgcta
    17941 ggcaacgttc tttccgccat cgcgccggat tatatgaccc tgatgctttc acgtattttg
    18001 accagcctga atcacggagc attttttggt ttgggttcag tcgtggccgc aagcgtggtg
    18061 ccaaaacata aacaggccag cgcagttgcc actatgttta tggggttaac cctggcaaat
    18121 atcggtggcg tgccggcggc gacctggttg ggtgaaacca tcggctggcg gatgtcattt
    18181 ctggcaacgg cggggctggg agtgatttca atggtaagtc tgttcttctc attacctaaa
    18241 ggtggtacag gggcacgacc tgaagtgaaa aaagagctgg cggtattaat gcgtccgcag
    18301 gtgctgtctg cattgctgac gacggtactg ggagctggtg caatgtttac cctctacacc
    18361 tatatctctc cggtactgca aagtattacc cacgcaacac cggtgttcgt cacggcaatg
    18421 ctggtgctga ttggtgtcgg attctctatc ggtaactatc tcggcggcaa actggcagat
    18481 cgttcagtta acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat tatgctggca
    18541 atcccgttcc tggcccgcaa tgagttcggc gcagctatta gcatggtggt gtggggcgca
    18601 gcaacctttg cggttgtacc tccgttacag atgcgcgtga tgcatgtcgc cagtgaagcg
    18661 ccaggtctgt cttcatcagt caatattggt gcctttaatc ttggaaatgc gctgggagca
    18721 gctgctggtg gtgcggtaat ttccgctggg ctgggataca gctttgtgcc ggtgacgggg
    18781 gcgattgtcg cgggactggc attattgctg gtgtttatgt cagccagaaa acaacctgaa
    18841 acagtttgcg ttgctaacag ctaattaaac aaaaacgcag aaggataacc ttctgcgtta
    18901 cagttgctga caacgtgcga tttatcttgc cgaatgcgct gttaacgcct catccgcgtt
    18961 ggcatggtaa gcgaagcgca tcaggcaatg ctgcatttgc catcagttat tatgcagcga
    19021 gatttttcgc tacgaattcc cagttcacca gcgcccagaa gtgctccaga tagccaggac
    19081 gtgcattgcg atagtcgatg taataagcgt gttcccagac atcaacggtc agcagcggag
    19141 tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga aacgatagcc agtttgccat
    19201 cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct gcatcagtaa
    19261 actgcgcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca gcgacttttc
    19321 cagtcggttc gccaccggcg ttcggtgcca ggcagttcca gtagaaagta tggttccaga
    19381 cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct tccagtgatt
    19441 taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa gtctgatgat
    19501 gcttgccgta gtgatactcg atagtttccg cagaaatgtg cggtgccaga gcatctttag
    19561 catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat taatttgcat
    19621 acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt gagacaaaag
    19681 atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt ttttggggtc
    19741 attacccgac gagcgccaac atagtggcgc tgccagtagt cttcactgag agaagtgatt
    19801 tgaatttcct gacctgtgcg cggtgactga ataaatttgc cgttgccgac atacacgccg
    19861 acatgatcgg ctgtaccgcg tccctgagta cggaaaaaga ccaggtcgcc gtttttcagc
    19921 tcactacgtt cgataggtcc tgcatcacgc aggtgataca tttcattcgc cgtacgcgga
    19981 atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca atcaaaaccg
    20041 gtacgcggtg agctgccgcc ccaacgatat ggcttaccaa tttgctgcat cagtttattc
    20101 attgccactt tcgtggcttt ttgcaccttc gctttgtgcg cgtcggcaag ggttcctgac
    20161 gcggcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt acacttttcg
    20221 gtaaagctgg ctgaggccgt tttattgacg gcgtgtttgc tgcgagtttt ggcgttttta
    20281 gaagcggtaa tggaagattt tttaacagaa gaagcggttt tgcttttggt catactttta
    20341 ctggcggctt ttttcgctgt tttctggctg gctttctggg tctttttggt ggttgccgtg
    20401 cttttctttt tatctgcttt tttggtgata tgagtggtag cagaactctc cctggcttgc
    20461 tttgaagcat gggccatagg cgttaaaggc agggtggtaa aaagtaaagc acagagcgtg
    20521 atcgaaatac ggtttatccg cgccactgag cgtccctctg ttaaggcagg tctatcatgc
    20581 ctgcgtctga tttacaaaac ttgatgattc taatgcatag aagaagagaa agttaatagc
    20641 ttttattgtt aaaaaatacg tttcgttggc gagcgcaaaa aaatgcagca gtgaaagcta
    20701 aaaatggtta taaatacgcc agtttgccgc gcatcagaga agaaacgaat atttgaaatg
    20761 caacaaatag agcaaaggga taaaatagca aaagcgtgat gaaaatgtta ttttccgatg
    20821 ccgcactgac ccgctacaat gtcagattac tgccgtaaag aagtttgagg aagcaagaca
    20881 atgagcacca ctatcgaaaa aatccaacgc cagattgctg aaaacccgat cctgctgtac
    20941 atgaaaggtt caccgaaact gccgagctgc ggtttctctg cccaggcagt ccaggcgctt
    21001 gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc agaatccgga cattcgtgcg
    21061 gaactgccga aatatgctaa ctggccgacc ttcccgcaac tgtgggttga cggcgagctg
    21121 gtcggcggtt gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca gcagctgatc
    21181 aaagaaactg ccgctaaata caagtctgaa gagccggacg cggaataatc cgtcctgcaa
    21241 tactgacgga tgcgaacggg catccgtcag tcctttatcc ggtgcgccgt aaaacctcgt
    21301 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    21361 atgtactggc ggatgacgga tattggcgca ccttcgcagc tactggcaaa atagccagga
    21421 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    21481 aacctattcg atatgccttt gaggctgttt accagactgg atatcgccag tttgggagga
    21541 tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagctgca
    21601 aaatcagcac atacatttga aaagtaagtg cgtagttttt ctgtcgtgtc atggtcaaaa
    21661 atctggcgtc ggtatttggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc
    21721 cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg acctgtgaaa
    21781 cgattacagg catttaaatt ccagttaaga cccggtggtc aacaggagcg tgaaatgagg
    21841 cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcgcttca gaatgagaat
    21901 catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg
    21961 aaaaacgcca ctgaaacgca atggcttaaa gattcgccct cacagccatt gcaacagtca
    22021 ctgaaagacc ttgagcgggc ttacaaaaac ttcttccaga agcgggctgc ttttccccga
    22081 ttcaaaaagc ggggacagaa tgctgcattc cgctacccgc aggacgttaa gctcgatcag
    22141 gaaaacagcc gtatttttct gccgaaactt ggctggatgc gctaccggaa tagccgtcag
    22201 gtcacgggag ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg gtacatcagt
    22261 attcagacag aaagtgaagt atcaactccg gttcaccctt cagcatcaat gatcgggctg
    22321 gatgctggcg tggctaaact cgccacgctg tcagatggca cagtctttga gcctgtaaac
    22381 agttttcaaa aaaaccagaa gacgctggcg agacttcagc gacagttaag ccg
//
LOCUS       DAZOZX010000058        21512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZOZX010000058 DAZOZX010000000
VERSION     DAZOZX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.058"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUPT_002201"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUPT_002201"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0819761.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUPT_002202"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUPT_002202"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0819762.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUPT_002203"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUPT_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0819763.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUPT_002204"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUPT_002204"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0819764.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUPT_002205"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUPT_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUPT_002206"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUPT_002206"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0819765.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUPT_002207"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUPT_002207"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0819766.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUPT_002208"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUPT_002208"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0819767.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUPT_002209"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUPT_002209"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0819768.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUPT_002210"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUPT_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0819769.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUPT_002211"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUPT_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0819770.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUPT_002212"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUPT_002212"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0819771.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFRSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUPT_002213"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUPT_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0819772.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUPT_002214"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUPT_002214"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0819773.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUPT_002215"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUPT_002215"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0819774.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUPT_002216"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUPT_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0819775.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="ACHUPT_002217"
     CDS             complement(18170..18565)
                     /locus_tag="ACHUPT_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0819776.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUPT_002218"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUPT_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0819777.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="ACHUPT_002219"
     CDS             19481..20074
                     /locus_tag="ACHUPT_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0819778.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUPT_002220"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUPT_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0819779.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="ACHUPT_002221"
     CDS             20794..>21512
                     /locus_tag="ACHUPT_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0819780.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5195 a         5324 c         5669 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccggt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAZOZX010000059        21504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZOZX010000059 DAZOZX010000000
VERSION     DAZOZX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.059"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHUPT_002222"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHUPT_002222"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0819781.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHUPT_002223"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHUPT_002223"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0819782.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACHUPT_002224"
     CDS             complement(3121..3420)
                     /locus_tag="ACHUPT_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0819783.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACHUPT_002225"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACHUPT_002225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0819784.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHUPT_002226"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHUPT_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR0819785.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHUPT_002227"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHUPT_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0819786.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHUPT_002228"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHUPT_002228"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR0819787.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHUPT_002229"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHUPT_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="ACHUPT_002230"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="ACHUPT_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0819788.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="ACHUPT_002231"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="ACHUPT_002231"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR0819789.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="ACHUPT_002232"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="ACHUPT_002232"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR0819790.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /gene="xylR"
                     /locus_tag="ACHUPT_002233"
                     /pseudo
     CDS             12687..13865
                     /gene="xylR"
                     /locus_tag="ACHUPT_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13973..14797)
                     /locus_tag="ACHUPT_002234"
     CDS             complement(13973..14797)
                     /locus_tag="ACHUPT_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR0819791.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="ACHUPT_002235"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="ACHUPT_002235"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0819792.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="ACHUPT_002236"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="ACHUPT_002236"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR0819793.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="ACHUPT_002237"
     CDS             complement(18729..19202)
                     /locus_tag="ACHUPT_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0819794.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHUPT_002238"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHUPT_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR0819795.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHUPT_002239"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHUPT_002239"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR0819796.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21504
                     /locus_tag="ACHUPT_002240"
     CDS             21362..>21504
                     /locus_tag="ACHUPT_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR0819797.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5403 a         5489 c         5277 g         5335 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgctgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgca
//
LOCUS       DAZOZX010000060        21342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZOZX010000060 DAZOZX010000000
VERSION     DAZOZX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.060"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            255..443
                     /locus_tag="ACHUPT_002241"
     CDS             255..443
                     /locus_tag="ACHUPT_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0819798.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(493..822)
                     /locus_tag="ACHUPT_002242"
     CDS             complement(493..822)
                     /locus_tag="ACHUPT_002242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819799.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(947..1126)
                     /locus_tag="ACHUPT_002243"
     CDS             complement(947..1126)
                     /locus_tag="ACHUPT_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819800.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1256..1456
                     /locus_tag="ACHUPT_002244"
     CDS             1256..1456
                     /locus_tag="ACHUPT_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR0819801.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            1446..1667
                     /locus_tag="ACHUPT_002245"
     CDS             1446..1667
                     /locus_tag="ACHUPT_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819802.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            1660..1880
                     /locus_tag="ACHUPT_002246"
                     /pseudo
     CDS             1660..1880
                     /locus_tag="ACHUPT_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1882..2115
                     /locus_tag="ACHUPT_002247"
     CDS             1882..2115
                     /locus_tag="ACHUPT_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819803.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            2121..2420
                     /locus_tag="ACHUPT_002248"
     CDS             2121..2420
                     /locus_tag="ACHUPT_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR0819804.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            2417..4165
                     /locus_tag="ACHUPT_002249"
     CDS             2417..4165
                     /locus_tag="ACHUPT_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR0819805.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            4445..4723
                     /locus_tag="ACHUPT_002250"
     CDS             4445..4723
                     /locus_tag="ACHUPT_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819806.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            4720..5025
                     /locus_tag="ACHUPT_002251"
     CDS             4720..5025
                     /locus_tag="ACHUPT_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819807.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            5035..5109
                     /locus_tag="ACHUPT_002252"
                     /pseudo
     CDS             5035..5109
                     /locus_tag="ACHUPT_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5184..5297
                     /locus_tag="ACHUPT_002253"
     CDS             5184..5297
                     /locus_tag="ACHUPT_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0819808.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            5338..5562
                     /locus_tag="ACHUPT_002254"
     CDS             5338..5562
                     /locus_tag="ACHUPT_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819809.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            5863..7020
                     /locus_tag="ACHUPT_002255"
                     /pseudo
     CDS             5863..7020
                     /locus_tag="ACHUPT_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            7060..7632
                     /locus_tag="ACHUPT_002256"
     CDS             7060..7632
                     /locus_tag="ACHUPT_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR0819810.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            7634..8845
                     /locus_tag="ACHUPT_002257"
     CDS             7634..8845
                     /locus_tag="ACHUPT_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0819811.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            8842..9180
                     /locus_tag="ACHUPT_002258"
     CDS             8842..9180
                     /locus_tag="ACHUPT_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR0819812.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            9177..9473
                     /locus_tag="ACHUPT_002259"
     CDS             9177..9473
                     /locus_tag="ACHUPT_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR0819813.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            9473..9913
                     /locus_tag="ACHUPT_002260"
     CDS             9473..9913
                     /locus_tag="ACHUPT_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0819814.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            9897..10079
                     /locus_tag="ACHUPT_002261"
     CDS             9897..10079
                     /locus_tag="ACHUPT_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819815.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            10135..10461
                     /locus_tag="ACHUPT_002262"
     CDS             10135..10461
                     /locus_tag="ACHUPT_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0819816.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            10445..12106
                     /locus_tag="ACHUPT_002263"
     CDS             10445..12106
                     /locus_tag="ACHUPT_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0819817.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            12120..12401
                     /locus_tag="ACHUPT_002264"
     CDS             12120..12401
                     /locus_tag="ACHUPT_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819818.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(13198..14658)
                     /gene="phoQ"
                     /locus_tag="ACHUPT_002265"
     CDS             complement(13198..14658)
                     /gene="phoQ"
                     /locus_tag="ACHUPT_002265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0819819.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(14658..15329)
                     /gene="phoP"
                     /locus_tag="ACHUPT_002266"
     CDS             complement(14658..15329)
                     /gene="phoP"
                     /locus_tag="ACHUPT_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0819820.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(15498..16868)
                     /gene="purB"
                     /locus_tag="ACHUPT_002267"
     CDS             complement(15498..16868)
                     /gene="purB"
                     /locus_tag="ACHUPT_002267"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0819821.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(16872..17513)
                     /gene="hflD"
                     /locus_tag="ACHUPT_002268"
     CDS             complement(16872..17513)
                     /gene="hflD"
                     /locus_tag="ACHUPT_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0819822.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(17549..18655)
                     /gene="mnmA"
                     /locus_tag="ACHUPT_002269"
                     /gene_synonym="trmU"
     CDS             complement(17549..18655)
                     /gene="mnmA"
                     /locus_tag="ACHUPT_002269"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0819823.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(18709..19170)
                     /gene="nudJ"
                     /locus_tag="ACHUPT_002270"
     CDS             complement(18709..19170)
                     /gene="nudJ"
                     /locus_tag="ACHUPT_002270"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0819824.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(19180..19833)
                     /gene="rluE"
                     /locus_tag="ACHUPT_002271"
     CDS             complement(19180..19833)
                     /gene="rluE"
                     /locus_tag="ACHUPT_002271"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0819825.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            20005..21255
                     /gene="icd"
                     /locus_tag="ACHUPT_002272"
     CDS             20005..21255
                     /gene="icd"
                     /locus_tag="ACHUPT_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0819826.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5585 a         5198 c         5642 g         4917 t
ORIGIN      
        1 tagctgattt ttaatgtgtt actgattttt tatacactag cgaatcactc tttaaatagc
       61 gagaaaaaag gcatgaaaca atattttatg cctttgtatt aacatgaatt taaaggattt
      121 tatcctgatt attcctcttt ctgtaaacca tacggcctca acatttgcca actctgaatc
      181 atcctgacac aatcagacac atcccaacgc aatagaacac gaaataactc tttaagaaac
      241 gaaagggggc aatagtgtta agcactgatc ggtttatacg tgaaaaagaa tgcgaaaagc
      301 taacaggcct tagccgctca tgccgctacc gcctggaaaa ggccggacaa ttcccatcac
      361 gtcgtaaact tggcggtcgt tccgttggct ggtctttatc cgaggttctg gcctggaagg
      421 atagctgcaa ggcagttcat taatcacgct ggcggcacgc agccgccaca catcaatcat
      481 ctgaacacag agctataacc atgaagattg aatatacgcc agaacgtggg cggggattcg
      541 ttcgccctgg tgaaactgga aaaccacaaa attggggttt ttcaggcata aaaaaagcgg
      601 ccccgaaatg gagccgcctt tctgaacaga taacccgctg cgccgtttgt gtgtgtgatc
      661 ccaaacataa gcacggggat gatagccgct atcaggctgg tgggcaatgc aatcagtctg
      721 gttcagtgcg ttgccataac tgcgaagttc tctcctttga tgagtattct ttaaggtact
      781 tctcaagggc aaaagaacac ggcgcgaatc gttctgattc atgctctttc tttctgcgtc
      841 gtctttttcg tgcaggtgat gttttttcgg ttgattcttc gttggtcatt gtgtgcgcct
      901 gtaaagcaat gcgccgtagt acctcacacc acggcgctga tagtttttat cctttgggtt
      961 ctatgccgcg ttgctgtaat tctttgcgga ttattcgctt tatccaggca gcaagagaat
     1021 catcgccgtc ctgttgttgt gccttttcca tcttttcccg taagtcagga tctaaacgga
     1081 actggaacgg aggattacca cgcctttcgt ttttgtgtgt tgacacgtta attacacctc
     1141 aggtaatgta tttatgtgta gtgacacatt acacacaatc aacacaaaaa gcaaagcccc
     1201 gcactgtagg agcagtaacg gggcttctaa ccaacaacgt aaactaggag ccgttatggt
     1261 tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt ggcgttttct
     1321 ggcactgagt gccggagaat cccaaatcat ccacgtaacc gcttggacgg aacgcgaagc
     1381 gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc gtcaggggga
     1441 aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact acgctgatgc
     1501 acgcgcttat tgatattgaa tgcacggcag agcttgcgca gggagaagaa cagaaagacc
     1561 ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcactg aatgacgcta
     1621 aaaatattct tgttgccgat tgtgaaaatg gggggggtta tgcgtgatga tcgttttaat
     1681 tccctgaaac aggaattttc cggcgttcct gatgatgcgg ctgatgcgct ttcgtcaatg
     1741 ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa atcaacgggc
     1801 ttgatgtact gaatatcgcc gccgattatg ctgatttcgt gacagaggtt attttaagaa
     1861 aaacgacgga cggggattaa tatgcgtgat atttaccacg aaacaataga ccgcgcattt
     1921 cttgcacttt ctcacagtga aaacatgctg gaaatattgc gcatatggct tgaaacactt
     1981 ggcgacaatg aacgcgacaa acaaaaatca agaattgcca cggcattaat aacgcttctt
     2041 gagcctgtaa taatggaact gcaagaaata gatctattgc acgacagata taaagaacaa
     2101 cacaccggag aataaaaata atgaaactta aatattctgg cttaactgcc agtggcaaca
     2161 ctcaccctaa atttacgcgc ggtgatattt accgcgacca gtacggcggc atggtaacga
     2221 ttaaaggcgt ggcagaacgg cgcatcacct accgccgtga aggttacgaa tatgattgcg
     2281 tgatgcctgt ttatcaattc cggcgtgatt tcacgctggt acaggcagcg ccccgcagta
     2341 aaccaaccag cagggagaaa gcacgcgcca atattcagga aataaaaaag atgcttaacg
     2401 tattcagggg taaaaaatga aactggcacc gaacgtaaaa aaacagccac gcggaataaa
     2461 acacaaagac acagaggtaa ttatctttgc gggtagtgat gcctggtcgc acgcgaaaca
     2521 atggcaggag caggatggcc ccgcatccgg cgataatgtg ccgcctgtgt ggcttgggcc
     2581 aaatcagctt gccgaacttg atgcactgaa aattgttccg gatggaaaaa aacgcgtaag
     2641 gctgtaccag gccggagaac tggatttggt ggagaccaaa aagattggtc agaagctggc
     2701 ggcggcagat attcaggacg caaattttta ccccgaagga atgcacgtcc agaagtgtga
     2761 aaactggcgg cgctatctga atgctgagcg tgaaaatatt gccgcagggc ttaccatgcc
     2821 ggagcagaaa aatacgcaac tggcacaaat ggcagacagt gagcgcgcgc agctgcttgc
     2881 cgagcgcttt gatggcgttt gtgtgcatca ggaaagtgaa atcgttcacg tatggcgcgg
     2941 cggggtatgg tgtccggtca gcacaatgga acttagccgc gaaatggtgg tgatctattc
     3001 agagcacagg gccactttca gcaagcgcgt aatcaataac gccgtggaag cgttaaaagt
     3061 tattgccgaa ccaatgggcg agccgtccgg cgatttgctg ccgttcgcca atggtgcgct
     3121 tgacctgaaa acgggggaat tttccccgca cacgccggag aactggatca ccacgcacaa
     3181 cggcattgag tacacgccac cagcaccagg ggagaacatc cgcgataacg cgctaaactt
     3241 tcataaatgg cttgagcacg cagccggaaa agaccagcgc aagatgatgc gtatatgtgc
     3301 cgcgctgtac atgattatgg cgaaccggta cgactggcag atgtttattg aggccaccgg
     3361 agacggcggg agcggtaaaa gtacattcac acacatagcc agccttctgg cagggaaaca
     3421 gaacacggta agcgctgaaa tgacatcgct tgatgatgct ggtggacgtg cgcaggttgt
     3481 cgggagtcgt cttatcgtcc tggctgacca gccgaaatat acaggcgaag gaacgggcat
     3541 caagaaaatc acgggcggtg accccgtgga aattaacccg aaatacgaaa agcgtttcac
     3601 ggctgtaatc agggcggtgg tgctggcgac caacaataac ccgatgatat tcaccgaacg
     3661 ggccggaggt gtggcacgtc gtcgcgtgat tttccgtttc gacaatatcg ttagtgaggc
     3721 cgaaaaagac agggagctac cggaaaagat tgcggctgaa atccctgtca ttatccgccg
     3781 attgctggcg aactttaccg actctgagaa agcacgggct ttactactgg aacagcgtga
     3841 cggtgatgaa gctctggcaa taaagcaaca gacggatccg gttattgagt tttgccagtt
     3901 cctgaatttt ctggaggaag cacgcggcct gatgatgggc ggcggtggcg attcagtgaa
     3961 gtacacgacc agaaacagcc tttaccgcgt ctatctggcg tttatggcgt acgcaggcag
     4021 gagcaaaccg ctaaacgtaa atgactttgg caaggctatg aagccagccg cgaaagttta
     4081 cggacatgaa tatattacgc ggaaagttaa gggagtaacg cagactaacg caataacaac
     4141 agacgagtgc gacgcgtttt tataattttt tgtaattgct gtctacctga gtaaagaaaa
     4201 atacatttaa ttcagtgcat taagttaggt agatagcctt tttttactgt ctacccacta
     4261 tctaccctct ctacctgatt ttacctgaat cagacaggga ggtagacacg gggtagatag
     4321 tggataaaag cactctaccc cgctgaaagc agcgccatta ctggcatgag ggccactaag
     4381 gtagataagg tagacaaggg gaggcacaac tcaaaacttt ttaaacgagg gggtaaaaat
     4441 aaatatgcac acttcaggga aatttaataa atcattcaaa aaacgcagag acagaacaga
     4501 accgaaatat cgcgcgttag acatgacaga gcacgcttta aaggtggcaa tcagaacgat
     4561 agaccgccac gcgggggaag gatacgcgaa agcacatccc gaactgataa gcgcattcat
     4621 gaccacgacg gcggcaaatt ttgccacgct gacagagcgg gagattgccg aagcggaaca
     4681 ggtaacaaca atcaacgtta aaaccggaga ggtggaatca tgagcgaacc aaaatttggt
     4741 gaaaaatttt acaagcacaa cggacggatc acgattctgc aaataagcgc agctacacca
     4801 ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg catcatggga gctttgtgtg
     4861 cttagttacc ctgaccgcga tgtgtatcag gatattctgc ctgtgatatc cactgacaaa
     4921 ggcatgaaac ccgtagacat caaaaacatg gggtttcagt gcgtaatgct cactgaaaaa
     4981 atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc actgaggtgg caatatgaca
     5041 gcacaaattg cagcttacgg gcagctggtg ggcgacccgc tggtaaaaca gagcagcaag
     5101 ggcacaccgg agccgccaac ggagaactac gcagacctga gaaatgcagt aaatcgcact
     5161 tacgcagaca tggggctgag caaattagca gagtaggaat atcagaacca caccacgcag
     5221 ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta tggagcacta ctggcttttc
     5281 gtcacggtcc ggcatagtta ctatctgaaa caaacagaca caacagagga aaacgcaatg
     5341 ccgatgaaat ttgatgagat acttaaacag cgtgataaat accatgctga caacatggag
     5401 acgatgaata tcactgatta ccgcgcattt ctggaaacgg gggcgctgat tgaaaaggat
     5461 catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc tggcagtaaa tcctgaacag
     5521 acagatgcat tgatagaatt tctgaaaggg atcagagact gagcacccat acagcacata
     5581 cagccggagc aatccggctt tttgttattt tttgtaaatt atttgttcgt ggttgttcca
     5641 cgttgttcac tgagcggatc ggcatatttt acccgaactg aatcatgatt attctcgccc
     5701 gtggtgccag gacgctgggg ccactttccc gcctgttaat gtgctcgcca atattcatta
     5761 ccaggcggga aaacgatcgg tgcgattact gatttcctta tgaaaaacgg ttgagtgttt
     5821 gccgcgtcct ggagttcctt acttaacccc aggacttttt ttatgcagag aataatcgaa
     5881 ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg acatgctgga gaacgctgaa
     5941 aaagaaaacc gcagtcttaa cgatgctgag ggcgcaaaat ttgacgaatt acgcgctaaa
     6001 gctgaatccc tcgataaaga catttcccgc cttgaagcca ttgcagacga agagcgcagc
     6061 aagccaggta aaagcagcca gaccactgac cccgcagaac tacgtaacta cattctgacc
     6121 ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg gtggttatac cgttatccct
     6181 gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat ccaccatgcg tcgcatctgt
     6241 accgtgaaga aaatcagcag caacgagttt aagcagcttg tttccgctgg cggtgcgacc
     6301 gttaaccacg gtgaagaggg taaggcacgc gaacagacca gcaccccgca gattaacgag
     6361 gtgagcatta agctgtatcc ggtctatgcg tacccgcgca ccacacagga aatcgtggat
     6421 ttttccgatg tggacatcct ttcatggctg acgggtgaga ttggcgacac cttcacggaa
     6481 accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa aagcaaaagg ttttttatcc
     6541 gtaccccgtg cagagaagaa cgacaaagag cgtgattttg gtacgttgca ggtaattaaa
     6601 ccttccgaat ctctggcgtg gacatctgcg gacccgctga tcgacctgaa atttgcatta
     6661 cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact ccacgacggc ggcaaaactt
     6721 cagaaggtga agaacgcgaa cggtgattat atctggcgcg accgtttaca ggcgggtgat
     6781 cctgatacgt tgctgggcct tccggtcgaa tatctggagt ttatgcctga taacgttatt
     6841 gccctgggtg acttcaaacg cggttactac attgttgatc acgaaacagg tgttcgcacc
     6901 agaccggaca acctgacaga accaggattc atcaaaattt tcacgcaaaa atatttaggc
     6961 ggtggcgtgg tggattcgaa cgcgatcaag attctggcac tgccggaaga cgacgattaa
     7021 cagcatacag aaggggctaa aaagcccctt tagtgtttta tgggtgaaaa aattatgaag
     7081 agtatggaaa tccggtcatc ggaaatcacc accagcggag ccggtacgct gacgggctac
     7141 gttgttcgct gggataagct ttcagaactg ttatggggtg agttttacga aaaattccag
     7201 cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg ttcgcggcct gtatgagcat
     7261 gaccacagca tgttactggg gcgcacccgt tccggcacgc tgaaactgga agaggacgaa
     7321 acagggttac gctttgaact gaccccaccg gataccagta cagggcgcga cgttatcgaa
     7381 ctggttaagc gtggggacat ctcggggatg agctttggct ttcgctcccg taaggatgta
     7441 tgggatacca caacagatcc atgcgtgcgc accgtgctgg tggcggaact gtacgaaatt
     7501 accgttacat cggtaccggc ttaccccgat tccggcgtgg agctggcccg ccgttccctg
     7561 tatgagcagc accccgaaaa aatgccgcgt gcggataatc gccgctggtg ggcggattta
     7621 gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag cagcgcagca tgacgcttga
     7681 tgaatttatg gcgctggccg gcacatcgaa cacgggggcg ggtgagtacg tatcatctgg
     7741 gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg gtcatctctg acgcggtggc
     7801 taccatgccg tgttacctgt acctggtacg caatgagaag gggaaggagg cccgcgaatg
     7861 gcttgattct catccggtcg atcacatcct caacgagcgc ccgaacgcgt ggcagacacc
     7921 ctaccagttt aagcgaatga tgatccgcta ctgcctgtta agtggtaatg cttatgcggt
     7981 tattcagtgg gggcgtgatg gttttccggc ggctttacat ccttacccgc cgcagtcggt
     8041 gaacgtggag cagacaggtg atcataactg gcgctactgc atcactgacg cctacaccgg
     8101 aaacatccgc aactatttac cgtgggaggt tctccacctt cgttactcca cggatgacgg
     8161 ttttatgggg cgctcacctg taaccatctg ccgcgaatcg ctggggcttg ggctggccca
     8221 acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg gcggcagggg ttatcacgtc
     8281 aggcgaatgg ctggacggcg tgaagggcaa acaggcatta gccgcactgg aacgctacaa
     8341 aggggccaga aacgccggaa aaacgcccat ccttgaaggg gggatgagct atcagcagct
     8401 gggcatgagt aatcaggatg ccgaatggct ggcctcccgt cgcttcacca ttgaagacat
     8461 cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa tacagcaaca gcacctacag
     8521 caacttcagc gaggcaagcc gcgcatttct caccatgacg atgcgcccgt ggctggcgaa
     8581 ctttgagcag cagataaaaa acgccttgct ggtggcctcg cctgtacctg gtatccggta
     8641 tcaggtggag tttgacagcg cggacctgtt acgggccaca cctggcgaac gctttgccac
     8701 ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac gaagcccgcg aacgtgaagg
     8761 gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca tggaaacagg aagtaaaaat
     8821 cagcgaggga gaaaaaccgg aatgaacata gggcgactgc gtgacaggat aacgattcag
     8881 accctgaaac agaccaggga tataaccggc gaaatactcg aaacgtggga ggacggtcac
     8941 acactctggg caagcgtgaa catggtcagc agcaaggagg ccatttcatc gggcgcagag
     9001 ctggcgattg gtaccgtaag gatctggata cggtaccgga aggacatcaa cgccaccagc
     9061 cggataaagg tcagtacggg gccgctggcg gggcgtgtac tgaatatcat cgggcagccg
     9121 ctgccggatg ccgccaggac acgccttgaa attctttgtc gtgagggcgc ggaaaaatga
     9181 cagaagaact tatcaccctg gaagaagtga aactccattg ccgcatcgat ggcgacgatg
     9241 aagaccagtt aatcagcgga tacattgccg catcgcttga ggcgtgccag atacacatag
     9301 gcaggcgctt tgatgacggg ctggcgttca cgccagccat aaagattggc tgcatgatgt
     9361 ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga taacgcaaaa acacgcgtac
     9421 ccatgacgat aggcgcactc tggacggctt accgtgatgt gggggggtac tgatgccgtg
     9481 gcaaccatta agacgatgca cagaaccagg ctgtaacagg cgcgttaaat ccggcaagtg
     9541 tgaggagcac aggcgggcgg catggcgtgc agaggatgcc agacgaggac accgccgcgc
     9601 gcgtggatat tccagacagt gggacaaata ccgcgccatg tatctgagta aaaacccgtt
     9661 atgcgtgcga tgccttgaga aggggatata tacgcccgcc gtggtggtgg atcacattat
     9721 cccgattaat ggcggtgatg atgttctctt ctggcccgaa tggaaccatc aaccattgtg
     9781 ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg gcaacaaaaa gcaagcgtgc
     9841 cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac cgcaataact ggatgtatga
     9901 cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg acttataagg cagcgtgacg
     9961 catggaagac acaggaaaca gggcataaag ataaagcgtc aggacgcgca gaacgcatca
    10021 cagcgaagcg attaaccgac cgtgaccgcg aggttatgga atgtttccgc aatcgctgat
    10081 gaggctgtat gacggggtgg ggggcgtttt caggacaaac ccgaccccgc ctacctggat
    10141 gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc ttgcggaacg gggagacctg
    10201 acccccgccg actggagcaa tctggaactg tattgcgtca actactccat ttaccggaaa
    10261 gccgttgcag accttgcggc gcgcgggttc agcattgtta acagtcaggg cggcgagagc
    10321 agaaaccccg cattaagcgc aaaatccgac gctgaaagag tgatgataaa aatggcctcc
    10381 ttgctcggtt ttgacccgat aagccgccgt aaaaatccac cggaaacaga agaagaggac
    10441 gagcttgacc gcctggaata agtacgcaga agacgtaaaa acgggcaaaa ttccggcctg
    10501 taaacggtta aaacaggctg ttaaacggta cttttcggac cttaaaagcc ccctttacac
    10561 gttcgatcgt gaggttgtgg agcggtttat tgccttttcc agggtgtgcc cgcacgtaaa
    10621 aggaccgatg cgtggcagac ccattgagct ggagccgtgg cagcagttcg cctttgcgtg
    10681 catcctcggc tttaaggtta aggccaccgg acggcgcaaa tacacgagcg cctttatcga
    10741 agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt ctggctaact ggtttctgat
    10801 tatggagaac ggtcagcagg atatctacac cgcagcggtg agccgtgacc aggcgcggat
    10861 cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga ccgttacgca ggcgggtgaa
    10921 tattcaggcg cataaggtga tacacccgaa aagcaacagc ctgttaaagc cgctggcagc
    10981 aaaagcggca accatcgagg ggacaaaccc cagtctttcc atcgtggacg aataccacct
    11041 acaccccgat aacggcgttt attccgcgct tgagctgggg atgggcgcac gtcccgaagg
    11101 gctgttattt gccattacca catcgggcag taacgtcgtt tcagcctgta aacagcacta
    11161 tgattattgc tgccagatcc tggacggcga agaggttaac gattcaattt ttgtactgat
    11221 ttacgaactg gacgacgaaa acgaggttga tgatccggcg atgtggataa aggcgaaccc
    11281 taatattgat gtttccgtgg atcgtgaaaa actggcctca accatccaga aagcgcgggg
    11341 tattccgtcg cagtgggtgg agatgatgac aaagcgattt aatatctggt gtcaggggac
    11401 taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc ggaacgttca cggaggaaga
    11461 tttacacggt caggagtgtt acgcggggct ggacttatca tcaaccagcg atatttccag
    11521 cgtatgctat gcctttccgg tcggtaaaac cattatgctg atttcccgtc attatctgcc
    11581 ggagttccag ctacagaacc ccgccaataa aaaccgcgct gtctatcgcc agtgggctaa
    11641 agcgggctgg atacgcacaa cgccaggtga ctgcattgat tacgaccgga tcagagatga
    11701 catcatgcag gatgcggaga aatttaatat caggctggtg ggctttgata cgtggaacgc
    11761 cacgcatctc aggacgcaat tacagggggc tggttttgag gtggagccgt tcccgcaaac
    11821 ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt tttgttaacc gcagggtgat
    11881 tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt aacgtcgtga tgcagagtga
    11941 cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg aacaagatag acccgagcgt
    12001 agcggcgctg atggcgtttg gtacattcca ggcagagcat gaggattttg cttttgatat
    12061 aagcgacagc caccgccaga aactggaaga atttagcggg gtgtaatgag gtcagcagca
    12121 tgacagaagc cgaaatactg cgattaatcc gccgcgtctc tggaatcagc cagcaggctg
    12181 acgaacagac cacgcagccg gacagcgtga cagccgaaaa ttatgcgcgt gttgttgctg
    12241 aggtgatgcg ccgtgatggt atccagctta atgatgcgga tatgcgcgac atacggatcc
    12301 gcgttctgga aatgctggcc tacaatcgcc gcgttgaact gtatcgggag aaggagaaaa
    12361 tcacgtacca ctggaagaag ccggagcggt tacggcggta actcactgag attaaagcaa
    12421 agcgcaaaat tgcgctggct acatccctca catctgagga ctgttaacca gcaggccgga
    12481 atatattcag aaactacgaa attttcgcag tttaattact cgtcagctta atcgctaacc
    12541 cgctctgata ttttcggaaa cctcaatttg aggaagtcgg cgaggtaact gtttgatatt
    12601 cccgatagcg caaagatgcg cggtcgggca gaatgaccac cacctcaatc gtgacggaga
    12661 gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa ccgttcatta aaaatcgacg
    12721 tgattttgtg ctttccggat gacgtggcca acgtcatttt ttagcgaaaa attctgatta
    12781 aggtcactgg ctgaacggtc ataatgacca cgcagaccat cgaggcaacc atagcgatca
    12841 tcgttacggt tggagctttt ccgccattgg tggaaaaggt gcacatctgg atcatcatta
    12901 cggtgatcaa gggtatgtaa aacccaccag cctgattaaa ggttaaccgg aaaaaaaatc
    12961 cggtagcccc gttatgggga tccccatatc gacattaacg cccctcatga attggtgcgc
    13021 ttccccctgg aaagattacc cgcctgtata tttcttgtgt ctatttgttc cacgttgttt
    13081 catacagtgc accgaacggt gtagttaatg gtgtagtcaa tttgcgattt ttggcacttt
    13141 ttcaatgaaa attgtttgat cgaatcaatg acttgatgta gtggtaaaag gacatattta
    13201 ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc tccatccgcg caccgcccag
    13261 catgctctct ccggcgacga ttttaccctc atattgctcg gtgatttcgc gggctaccgc
    13321 cagccctaca ccttgcccag ggcgtaaagt atcaacccgt tgaccacggt cgaaaatgac
    13381 ctctcgcttg cttaatggaa taccggggcc atcatcctcg accacaatat agagatgctc
    13441 gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa tatttacagg cattatccag
    13501 cacgttgccc atcacctcga caaaatcgtt ctgctcaccg acaaagctga tctctggcga
    13561 aatatcgaga gagatattga cccctttgcg ttgatacact ttgttcagcg ctgaggtgag
    13621 attgtccagc agtggggcga ccggatgcag ctcgcggctg agcaatgtcc cgccgcgcat
    13681 actggcacga tgcaggtagt agccaatttg ctgtgaaatg cggctgattt gctccagcat
    13741 taccggctca gcatcactga cgctcatctt ttcactacgc agagaacgca gcgtactttg
    13801 cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg gtgagcgtcg tacggtattt
    13861 gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg tttcgtacca gactggtcag
    13921 ttctcgcgtt gtggctggat tgagcaattc gcggttatgt tcttccagtt cgcggacttc
    13981 ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg gcgacccaca gcagcgggat
    14041 cactaacagc agattggctg agagcacata gataaaccag ctccagacca tataggaact
    14101 ttttagctcc accggaatgg tatccaccac cacaatggtt aattttggca tccgcgatgt
    14161 tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc gcgtcgtcat catcttcccg
    14221 cacttcctgc aactgttgct gtatcgaatg atctccactc agcaagaggc tggtatcgtt
    14281 aacatccgct tcaatttcat gaaaaccatt cgatttcagc cagtcaggct ggatcatttt
    14341 catcagccag ggcacgtcac gttgcgccca taaaagctgc ccgttctcat cataaattag
    14401 cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac tcgacatgca acttattgtt
    14461 ttcccactgc gcaagggtat agaacagatt gctctcgcca cgtaacagcc gaaacgtagt
    14521 tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt ccgtaggcaa gcgaaagcac
    14581 cagtactacc gctgccgttg ccaacagaaa acgtacccgc agcgagagcg ggaaaaaaag
    14641 acgcagtaat tttttcatca gcgcaattcg aacaggtagc cctggccgcg aacggtggta
    14701 atgacttctt gcggatattg tgcctgaatt tttttgcgca gacgtcccat cagtacatca
    14761 atggtatggc tttcccgcag ctccgcatcc ggatagagtt gtagcattaa cgaatctttg
    14821 ctgaccactt tgccattatt gcgtatcaac gtttccataa tggtgtattc gaacgcggtc
    14881 agtttgatca cttcgtcatt aatagataat tcacggcgag agagatcaac ctgaaacggg
    14941 gggagcgaaa tgacctgtga agccagaccg ctattacgcc gcattaatgc ctgcattcgc
    15001 gccatcacct cttcaatatg aaacggttta gtcacgtaat catcagcacc ggcacttaat
    15061 acttcgactt tgtcctgcca gctttcacgg gcggttaata ccagaatcgg cagtgaaaca
    15121 tcgttgctac gccagcggcg aatcagtgac agaccgtcct cgtcaggcaa tccgagatcg
    15181 acaatcgcaa tatccggtaa atgttcattg agataataat cggcttcttt ggcatcttct
    15241 gcgtcatcga cctgatgacc agcatcctga atctgaactt taaggtggtg acgtaacaac
    15301 gcattgtctt caacaaccag tacgcgcatt tttatttctc cctgtcttaa ttattaaaat
    15361 agtgtaacgc gattatgtgt ttatgggggt aaacattaaa taaaccagcg gggaggggag
    15421 gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca ggccggacag catcgccatc
    15481 cggcactgat ttgaggctta tttcagctca tcaaccatcg tgatagctcg accaatatag
    15541 ttagccggcg tcatcgcttt caggcgggct ttctcttctt ctggcaacgc cagaccatcg
    15601 ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc gagtaagctc tttcagcttc
    15661 tcgtacggtt tttcgatgcc atagcgacgc ataactgtct ggattggttc tgccagcact
    15721 tcccagttgt gatccagctc atccagcaga tggtcacggt tcacttccag tttgctcacg
    15781 cctttcaggg tggattgata cgcaatcagc gcataaccga tacccacgcc gaggttacgc
    15841 agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa ccggcagttt gcttgccaga
    15901 tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg agttttcgaa gtcgatcggg
    15961 ttaactttat gcggcatggt ggaagaacca atctcaccag caatggtttt ctgtttgaag
    16021 tggttaaggg cgatataacc ccagacgtca cggtcaaagt cgatcagaat ggtgttgaag
    16081 cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg gttcaatctg ggtagtgtac
    16141 gggttccatt gaatacccag cgaggtgacg aactcttcgc tgaactgatg ccagtcaact
    16201 tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg cgccgttgat tttgccgagg
    16261 atctccacct ggttaagctg gcggtgctgg cgctccatac ggtaggcgac gtttgccatc
    16321 tctttaccga tggttgacgg cgtggctggc tgaccgtggg tacgagacag cagcgggata
    16381 tcgcggtact gaacggcgag atctttaatg ccatcaatca gttgacgcca gtacggcagg
    16441 atcacttcat cacgcgcggt tttcagcatt aatgcgtggg agaggttatt gatatcttcc
    16501 gaagtacagg caaagtggat gaattcagaa accgcgtgca gttccgggat ctccgccact
    16561 ttttctttca ggaaatactc aaccgcttta acgtcgtggt tagtggtacg ctcgatggtt
    16621 ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa ttgcatcaag gtaaccgatt
    16681 gcgtcggcag caaaagcagg aacttccttg atcgctgcgt gcgcggccag tttttgcagc
    16741 caacgtactt caacttgtac acggaatttc agcaaaccat attcgctgaa aatcccgcgc
    16801 agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg aaacggcggt cagtgaggat
    16861 aattccatag atcacaactc cggggttaaa tgagcaagaa tttgttttgc ctgagtggtc
    16921 aggcgattac gagaaaacat cagttgtaga cgtccgccgc cgacctggtg ccagagcacg
    16981 gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg cctgcacttg tgggctttgc
    17041 agtacagcag gggaaccggt gacctgaatg cgcgggccaa gcgggctgat cacatcaaca
    17101 tagatagcag ccatcgcgct catcagcgtt tcggactgta aatcgaagtg ttcgagctgg
    17161 cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg cgcctttcgc tgaggagagt
    17221 ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta attcggcgtt taagccctgg
    17281 caactgctgg cattgagcac gccgagcagg gtttccagcc cgacgcgcag gttggcttcg
    17341 ctaccgccaa aaaccgccag cgtcgagctg gggttcatat caataatact gttgagtgag
    17401 acgtgtagcg catcggcatc acaatgcccc tggtgagcga gttgttgcac caggcgtgcc
    17461 gactgacaaa taccggccag ggcgagggtg atgtcatagt aattctttgc cacgttcact
    17521 gcttccttgt tttaagtaaa gataataatc agaccggcag cggcagacgc tgctcaataa
    17581 taccgccacc gaggcacact tcaccgttat agaagacggc agactggccc ggcgtcacgg
    17641 cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc gtccagcgcc ttgacggtgc
    17701 acggaatgtc ggtctggcga tagcgggttt ttaccgtgca acgcatagtg ccggtgaatg
    17761 gttcgcgatc gacccagtgc agctgctggg caatcaaccc gacagacatc agccgcgggt
    17821 gttcatggcc ctgagcgaca accagaatgt tgttttcgac gtctttgtcc accacatacc
    17881 acggttcttc ggtaccttct ttggtgccac cgatacccag acctttacgc tgaccgagag
    17941 tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc atcgacggta atgattttgc
    18001 ccggttgcgc cgggagataa cggcccagga actcgcggaa tttacgttcg ccaatgaagc
    18061 aaatgccggt agagtctttt ttcttcgcgg tgaccagacc aagatcttca gcaatcttac
    18121 gcacctgcgg tttttccagt tcgcctaccg ggaacaggct ttgcgcaatc tgctcatggc
    18181 tgagcgtata aaggaagtag ctctggtctt tattgctgtc cagaccacgc agcaggcggc
    18241 tcttgccatc gacatcggcc cgacgtacgt aatgaccggt agcgatataa tcggcaccta
    18301 aatcttcggc ggcaaattcg aggaaggctt taaatttgat ctccttgttg cacagaatat
    18361 ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa cagttcgaag acgttgtccc
    18421 agtactcggc agcaaagtta acggtgtgca gttcaatgcc gagcttgtcg cagaccgcct
    18481 gggcatcagc cagatccgct gctgctgtgc aatattcctc accgtcgtct tcttcccagt
    18541 tcttcataaa caggccttcg acctgatatc cctgttgttg caacagccag gcagaaacgg
    18601 aggaatcgac accgccggac atgccgacga ttactttttt tgcggtttca gacattggat
    18661 cactcacgac attgaacttc aaggcggcgt attctatcac gcagcctttt agatgacacc
    18721 ctttgtaaaa ggccagttaa aatcgccaat catctccagc ggataacgtt gcccgctttg
    18781 ataacaacga atactttccg ccaccagcgg cgaacgaaga tttgacgcct gtaaaatttc
    18841 ttcggcgctg acccaacggc agcagtcgat atcgctgtca tgaggctggg tcgggcatat
    18901 ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc gttttatctg gcgcaatcca
    18961 ctgatgcata cgaataaagt gttgcggctg cgcgctgatg ccggtttctt cccacagttc
    19021 acgggcggcg gcttccacta aggtttcatc ggcttccaga tgcccggcag gttggttcca
    19081 taacgcttta ccattaatcg tctcttcaac gactaaaaat ttgccttctg cgtgcaccac
    19141 gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct taatctgtca cttctcgcca
    19201 ttcgccattg gcaagattat ccaaagagta atctcccatc gcatagcgaa tcagtcgcag
    19261 cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg cgattacgtc cttcatataa
    19321 ggtgatcttc agccagctgg tgggaatact tttgcgttca cgaattggtg gattccgcgg
    19381 ccataaccac gcgggttcgt caaccagctc cgcgcctgcg ggcagggtag ggccatcatt
    19441 taaggttacg ccattgcgca aggcttcaag tgcgtcttgt gtgggaatac cttccacctg
    19501 cacataatag atttttccgg tgcgtttacc cggctgggtt aaacgcgcct gcaacgcgcc
    19561 gttattggtc agcaccagca acccttcgct atcgcggtca aggcgacctg ctgcataaac
    19621 gccctgaacc gggatgaatt cttttaatgt tttgcgtccg gtttcatcgg tgaactgcgg
    19681 aagaacatcg tagggtttat tgaagaggat cacacgcgtg ggctggtttt catgtttacg
    19741 cctggtagaa cgttgcgagc tgaatcgctt aacctggtga tttctaaaag aagttttttg
    19801 catggtattt tcagagatta tgaattgccg cattatagcc taataacgcg catctttcat
    19861 gacggcaaac aatagggtag tattgacaag tcaattacaa atcattaaca aaaaattgct
    19921 ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac gtggtggcag acgagcaaac
    19981 cagtagcgct cgaaggagag gtgaatggaa agtaaagtag ttgttccggc acaaggcaag
    20041 aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa atccgattat cccttacatt
    20101 gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc tgaaagtggt cgacgctgca
    20161 gtcgagaaag cctataaagg cgagcgtaaa atctcctgga tggaaattta caccggtgaa
    20221 aaatccacac aggtttatgg tcaggatgtc tggctgcctg ctgaaaccct tgatctgatt
    20281 cgtgaatatc gcgttgccat taaaggcccg ctgaccactc cggttggtgg cggtattcgt
    20341 tctctcaacg ttgccctgcg tcaggaactg gatctctaca tctgcctgcg tccggtacgt
    20401 tactatcagg gcactccaag cccggttaaa caccctgaac tgaccgatat ggttatcttc
    20461 cgtgaaaact cggaagacat ttatgcgggt atcgaatgga aagctgactc tgccgacgcc
    20521 gagaaagtga ttaaattcct gcgtgaagag atgggcgtga agaaaattcg cttcccggaa
    20581 cattgcggta tcggtattaa gccgtgttct gaagaaggca ccaaacgtct ggttcgtgca
    20641 gcgatcgaat acgcaattgc taacgatcgt gactctgtga ccctggtgca caaaggcaac
    20701 atcatgaagt tcaccgaagg cgcgtttaaa gactggggct accagctggc gcgtgaagaa
    20761 tttggcggtg aactgatcga cggcggcccg tggctgaaag ttaaaaaccc gaataccggc
    20821 aaagagatcg tcattaaaga cgtgattgct gatgcattcc tgcaacagat cctgctgcgt
    20881 ccggctgaat atgatgttat cgcctgtatg aacctgaacg gtgactacat ttctgacgct
    20941 ctggcagcgc aggttggcgg tatcggtatc gcccctggag caaacatcgg tgacgaatgc
    21001 gccctgtttg aagccaccca cggtactgcg ccgaaatacg ccggtcagga caaagtaaac
    21061 cctggctcta ttattctctc cgctgagatg atgttacgcc atatgggctg gactgaagcc
    21121 gcagacctga ttgttaaagg tatggaaggc gcgatcaacg ccaagaccgt aacctatgac
    21181 ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt cagagtttgg tgatgcgatc
    21241 atcaagaata tgtaatcact acatgtgtta aatatttaaa cgggcgtata acacgcccgt
    21301 tgttttattt atgtggatat tattaatagc atatcgagca ta
//
LOCUS       DAZOZX010000061        21275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZOZX010000061 DAZOZX010000000
VERSION     DAZOZX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.061"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..444)
                     /locus_tag="ACHUPT_002273"
     CDS             complement(<1..444)
                     /locus_tag="ACHUPT_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0819827.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="ACHUPT_002274"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="ACHUPT_002274"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0819828.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="ACHUPT_002275"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="ACHUPT_002275"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0819829.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="ACHUPT_002276"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="ACHUPT_002276"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0819830.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3514..3672
                     /gene="yoaL"
                     /locus_tag="ACHUPT_002277"
     CDS             3514..3672
                     /gene="yoaL"
                     /locus_tag="ACHUPT_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0819831.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="ACHUPT_002278"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="ACHUPT_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0819832.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="ACHUPT_002279"
     CDS             complement(5214..6812)
                     /locus_tag="ACHUPT_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0819833.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="ACHUPT_002280"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="ACHUPT_002280"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0819834.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="ACHUPT_002281"
     CDS             complement(8491..9069)
                     /locus_tag="ACHUPT_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0819835.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="ACHUPT_002282"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="ACHUPT_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0819836.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="ACHUPT_002283"
     CDS             10508..10687
                     /locus_tag="ACHUPT_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0819837.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11106)
                     /locus_tag="ACHUPT_002284"
     CDS             complement(10807..11106)
                     /locus_tag="ACHUPT_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0819838.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="ACHUPT_002285"
     CDS             complement(11528..11872)
                     /locus_tag="ACHUPT_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0819839.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="ACHUPT_002286"
     CDS             12004..13914
                     /locus_tag="ACHUPT_002286"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR0819840.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="ACHUPT_002287"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="ACHUPT_002287"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0819841.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="ACHUPT_002288"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="ACHUPT_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0819842.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="ACHUPT_002289"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="ACHUPT_002289"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0819843.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="ACHUPT_002290"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="ACHUPT_002290"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0819844.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACHUPT_002291"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACHUPT_002291"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0819845.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="ACHUPT_002292"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="ACHUPT_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(20410..21018)
                     /locus_tag="ACHUPT_002293"
     CDS             complement(20410..21018)
                     /locus_tag="ACHUPT_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0819846.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21275
                     /locus_tag="ACHUPT_002294"
     CDS             21188..>21275
                     /locus_tag="ACHUPT_002294"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0819847.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5265 a         5273 c         5465 g         5272 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt tttctgcgct
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DAZOZX010000062        21255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZOZX010000062 DAZOZX010000000
VERSION     DAZOZX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.062"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..793
                     /locus_tag="ACHUPT_002295"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACHUPT_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHUPT_002296"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHUPT_002296"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0819848.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHUPT_002297"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHUPT_002297"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0819849.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHUPT_002298"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHUPT_002298"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0819850.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHUPT_002299"
     CDS             complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHUPT_002299"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0819851.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHUPT_002300"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHUPT_002300"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0819852.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="ACHUPT_002301"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="ACHUPT_002301"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0819853.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHUPT_002302"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHUPT_002302"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0819854.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="ACHUPT_002303"
     CDS             complement(12980..14320)
                     /locus_tag="ACHUPT_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0819855.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHUPT_002304"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHUPT_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0819856.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="ACHUPT_002305"
     CDS             complement(16108..16557)
                     /locus_tag="ACHUPT_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0819857.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHUPT_002306"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHUPT_002306"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0819858.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="ACHUPT_002307"
     CDS             complement(17357..17686)
                     /locus_tag="ACHUPT_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0819859.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="ACHUPT_002308"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="ACHUPT_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0819860.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="ACHUPT_002309"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="ACHUPT_002309"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0819861.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="ACHUPT_002310"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="ACHUPT_002310"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0819862.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5671 c         5068 g         5288 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 tcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccatcata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc actggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcc
//
LOCUS       DAZOZX010000063        20712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZOZX010000063 DAZOZX010000000
VERSION     DAZOZX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.063"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHUPT_002311"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHUPT_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0819863.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHUPT_002312"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHUPT_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0819864.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHUPT_002313"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHUPT_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0819865.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHUPT_002314"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHUPT_002314"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0819866.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACHUPT_002315"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACHUPT_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0819867.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACHUPT_002316"
     CDS             complement(8217..9110)
                     /locus_tag="ACHUPT_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0819868.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHUPT_002317"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHUPT_002317"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0819869.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHUPT_002318"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHUPT_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0819870.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHUPT_002319"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHUPT_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0819871.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHUPT_002320"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHUPT_002320"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0819872.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHUPT_002321"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHUPT_002321"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0819873.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACHUPT_002322"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACHUPT_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHUPT_002323"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHUPT_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0819874.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHUPT_002324"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHUPT_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0819875.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHUPT_002325"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHUPT_002325"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0819876.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHUPT_002326"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHUPT_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0819877.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5727 c         5031 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAZOZX010000064        20701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZOZX010000064 DAZOZX010000000
VERSION     DAZOZX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.064"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHUPT_002327"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHUPT_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0819878.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHUPT_002328"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHUPT_002328"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0819879.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUPT_002329"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUPT_002329"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0819880.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUPT_002330"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUPT_002330"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0819881.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUPT_002331"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUPT_002331"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0819882.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUPT_002332"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUPT_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0819883.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUPT_002333"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUPT_002333"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0819884.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUPT_002334"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUPT_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0819885.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUPT_002335"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUPT_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0819886.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUPT_002336"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUPT_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0819887.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9182..11212)
                     /gene="rsxC"
                     /locus_tag="ACHUPT_002337"
     CDS             complement(9182..11212)
                     /gene="rsxC"
                     /locus_tag="ACHUPT_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0819888.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(11205..11783)
                     /gene="rsxB"
                     /locus_tag="ACHUPT_002338"
     CDS             complement(11205..11783)
                     /gene="rsxB"
                     /locus_tag="ACHUPT_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0819889.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11783..12364)
                     /gene="rsxA"
                     /locus_tag="ACHUPT_002339"
     CDS             complement(11783..12364)
                     /gene="rsxA"
                     /locus_tag="ACHUPT_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0819890.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12441..12881)
                     /locus_tag="ACHUPT_002340"
     CDS             complement(12441..12881)
                     /locus_tag="ACHUPT_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0819891.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(12967..13182)
                     /gene="ydgT"
                     /locus_tag="ACHUPT_002341"
     CDS             complement(12967..13182)
                     /gene="ydgT"
                     /locus_tag="ACHUPT_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0819892.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13455..13580)
                     /gene="blr"
                     /locus_tag="ACHUPT_002342"
     CDS             complement(13455..13580)
                     /gene="blr"
                     /locus_tag="ACHUPT_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0819893.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            13823..14863
                     /locus_tag="ACHUPT_002343"
     CDS             13823..14863
                     /locus_tag="ACHUPT_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0819894.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(14899..15900)
                     /gene="add"
                     /locus_tag="ACHUPT_002344"
     CDS             complement(14899..15900)
                     /gene="add"
                     /locus_tag="ACHUPT_002344"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0819895.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16004..17176)
                     /gene="malY"
                     /locus_tag="ACHUPT_002345"
     CDS             complement(16004..17176)
                     /gene="malY"
                     /locus_tag="ACHUPT_002345"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0819896.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17186..18778)
                     /gene="malX"
                     /locus_tag="ACHUPT_002346"
     CDS             complement(17186..18778)
                     /gene="malX"
                     /locus_tag="ACHUPT_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0819897.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            18953..19981
                     /gene="malI"
                     /locus_tag="ACHUPT_002347"
     CDS             18953..19981
                     /gene="malI"
                     /locus_tag="ACHUPT_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0819898.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20093..>20701
                     /gene="hdhA"
                     /locus_tag="ACHUPT_002348"
     CDS             20093..>20701
                     /gene="hdhA"
                     /locus_tag="ACHUPT_002348"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0819899.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5298 a         5207 c         5305 g         4891 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tctttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
     9301 gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggca gctttgcgcg
     9361 gatcaacctg ttcttctggc tccgcattgg cctgttgctg ttccagcttg cgtgctttgg
     9421 cgcgggcaat agcggcttcg acggcggctt tgcgcggatc gacctgttct tctggttccg
     9481 cattagcctg ttgctgttcc agcttgcgcg ctttggagcg ggcgatagct gcttcaacgg
     9541 cagttttacg tggttcagca acggttgctg cgtcgttagt ttgctccagt tctgcctgtt
     9601 tcgctctggc ttgcgcttta cgggcttccc gttctgcaat aattgcactg ttatccgggc
     9661 gttcgcccgc tttaatcaca ataggctgtg tagcctgggc ctgtttctct ttcacccgcg
     9721 ccagagcggc agcaatcgca tctttatctt tggctgcagg ttgaacggct gcgctcttat
     9781 gtcgttcaag gcgagtcgct ttttcgcgct ccagacgagc ctggcgcgct tcgaaacgcg
     9841 ctttggcttc tgcggcgcgc ttttcttcct gacgaatagc cgcaatttca gctttttcct
     9901 gacggaaata ttgcaccaag ggaatattgc tcgggcaaac ccacgcgcaa gccccacatt
     9961 caatgcagtc ggcaatgtta tgcgtggtag ctttatcgtg ttgctgacct ttgctgaacc
    10021 agtacaactg ttgcggcaaa agatccacag ggcaggcgtc agcacaggcg ctacaccgga
    10081 tgcagctttg ttcttcctgt ggttcgccaa gttcattggc agagggagcc aacagacagt
    10141 tggtaatctt tacgaccggg acatccagcc atggcaaggt aaagcccatt agcgggccac
    10201 ccataatcac catttgatcg gcagaggggc agaatccggc atcattcaat aaatgacgca
    10261 ctggcgtccc cagccgtgcc cagacgttgc ccgggcgagc gattgcttcg ccagtcaggg
    10321 ttacaacacg ctcggtaatc ggctcgccat caataacggc acgtttcact gcataagcag
    10381 tgccgacgtt ttgcattaat acgccgatat cggatgaacg cccgccatgt ggaacctgct
    10441 tcccggtcag aatgtaggtt aattgtttag caccgccaga aggatatttg gttggaatca
    10501 cccgcagaga aatatcgtta gagtccgcca gcaccgcgcg cagcatggaa atcgcctgcg
    10561 gtttgttatc ttcaatgccg ataagaattt cgcgtggctg cagaatatgc gcaagaatgc
    10621 gaataccctc tacgacctga gccgcgcaat cctgcatcaa acggtcatcg gcggtaatgt
    10681 acggctcgca ctcagcagcg ttgataatca acgtttcaat cttatctcca ccaccctgca
    10741 atttaacgcc tgtcgggaat ccggcaccgc ccagcccggc aacaccaaac tgatgtatgc
    10801 gctcgattaa ctcttcgcga ctgcgagagc gataatcgac ccagccgtcg cgcgggatcc
    10861 agcagtcttc accatcggca tcaataatca cgcttaattc agctaaagct gaaggatgag
    10921 ccgtagagtg gggcgcaata gccgtaacgg tacccgaggt gggcgcgtga acaggcagca
    10981 ttttgccgcg accacgggta agcggctggc cgcgcaatac tttatcgccg acgctaacgc
    11041 acaactcacc ttcagcgcca atatgctgtt tcagtggaat aacaaaatgc tgcgccagta
    11101 gtacctggcg caggggggta ccgttggtct gggttttcat ctccggtgga tggatgccgc
    11161 cgttgaaatc ccagatttta ttttttctga atgcagagaa taacttaagc atggtgttcc
    11221 acgggaatga tacgcacggg aatggtgttc agatcccatt tccaggagtc aggtgtttct
    11281 gcgaccggtt gcaacgagat gcagtgcgtc gggcacggat caacacataa attgcagccc
    11341 gtacagagat cactcattac cgtatgcatg acacgggtag cgccaacgat ggcgtctacc
    11401 ggacaagcct gaatacattt agtgcagcca atacagttat tttcatcaat aaccgccacc
    11461 atccgcgcag gcgttagctc ttgcgcttcg ccatccagcg gctgcggctc gacattaagc
    11521 aactcggcaa tttttagcat cacagcttcg ccacctgggg cgcaacggtt gattttttca
    11581 ccgttacagc tgatggcttc cgcgtagggg cgacagccgg gataaccgca ctgaccacac
    11641 tggctctgcg gtaagatttc gtcaattttc tcaacgaccg gatcgtcttc caccgcaaaa
    11701 cggcgggagg cataacccag aatggcgcca aacgccaggc ccagcaggct cacggcggca
    11761 acggcaatcc agatagcatt cattacaact tcaccaaacc actaaagccc ataaaggcca
    11821 gagacataag acctgcggta attaacgcaa tggcattacc gcgaaaaggt gccgggacat
    11881 cagccacagc aaggcgttcg cggatggcgg cgaagagcac catcaccagc gagaaaccga
    11941 cagcggcgga aaaaccgtac agcgccgact gcaagaaatt gtgcccgaga ttgatattca
    12001 gcaacgccac gccgagcact gcacagttgg tggtgataag cggcaaaaaa atccccagca
    12061 gacggtaaag caccgggctg gttttgcgca ccaccatctc ggtgaactgc acgaccacag
    12121 caatcaccag aataaatgcc agggtgcgca ggtaaatcag attaagtggg atcaaaatcc
    12181 acgtatcgat aagccaggcg caaatagacg tcagcgtcat cacaaacgtt gttgccagcc
    12241 ccatgcccat cgcggtttcc agttttttgg aaacccccat aaacggacag agaccgagaa
    12301 acttgaccag tacaaagtta ttgaccagta cagttccgac aaacagtaac aggtagtcag
    12361 tcatttttcg gcctgaaata aaaaagccgc ctattatccg ttaatccaga gcaggcgaca
    12421 acaggttaac tgtaaggtta ttacggattc acaaacgtcg cttttacccg cgacgagcgt
    12481 ttgaaatagg ggacgatcaa tgcggttgcc aacaaagtaa acagcaactg gcgaacggct
    12541 atgccaactt ccacgggtga aaaagcgaat gcttttactg ccagcaatac ggaaataagc
    12601 agccagataa tatagtgttt aggaacacaa cgccgacgtt taaagaaagc gatggtcaac
    12661 cacagtgtgt aataccacat ggcgatagcg gtaataaaag aaacgcccca taagatttgt
    12721 gtcgtcagtg cttgtccgcc gagcgtttga aatgtttgcg gagaagataa cgcagcggta
    12781 tacagcaaca acgccagcgt cgtactcaac agagcgacta aaagccaggc caacgggcca
    12841 agcaaccagc ctccaatacg ttgtggcgtt gtggtggtca taggttctcc agaatagcga
    12901 cgcaaacagg caaatctaat tttaggggcg ttattataag gcttttataa ccgatcgcta
    12961 ctttctttat tggacatagt gccagacgga cttcggtacc tggccgaggt caaacaaacg
    13021 cccgccagaa accagctctg cgcgacggtg atcggcagca cgatacatat tgatcagttc
    13081 ctgatcgtcc gtcagggtgt aattaagatg gtcgtagagt ttttccagac tttcgagtga
    13141 actgattttg cgaaatttta ataagtagtc ctgaacagtc ataaaaggtc cataaaataa
    13201 ttaataccaa aggggtaggt attattagtg atgataaaat ttcaccaaaa gtgaacgctt
    13261 gtgttattac aataataaga atgcgctgat ggcaaatatt cagggatgaa aaactgctct
    13321 gaaaagagta acgcgcttta ctatccctga caatcaccaa caacatcgaa caagataata
    13381 aattcctggt ttaatatccg acaagtgaaa acatgcaccc ggacgggcag catgtcgctc
    13441 cacaagtgca gagcttactt gtgttgtacc gaagcactct gttcaggtgg ctgatagttg
    13501 tcaatgtgac tcgccacgcc aagaagaatg actgaaacga caagaacgat ccaacctgtt
    13561 aattcaataa gacgattcat tacagcccac atctctcttg attgatccat taacttcagg
    13621 gggtaaatgt tacttagcaa taatagctcc agcaagattt ttactgaggt ttttgcgata
    13681 tttagctttt gtcgttggaa aattcgctat ttttttgact taagttaaac aacatccctt
    13741 attgctggca ggttattaaa ctgttgagcg tgggtaaagg atagtgtcaa atagccatca
    13801 tacttcaatg agaggcaatg acatgagcga caacatccgt gttgggttga ttgggtatgg
    13861 ttatgcgagc aaaaccttcc atgcgcccct gattgcgggc acgcccgggc tggaactggc
    13921 ggtaatctcc agcagtgatg aaacaaaagt aaaagccgac tggccaacgg ttacggttgt
    13981 ctctgagccg aagcatctgt ttaacgatcc caacatagac ctgattgtca ttcctacacc
    14041 caacgatacc catttcccgt tagccaaagc ggcgcttgag gcgggtaaac atgtggtcgt
    14101 tgataaaccc tttaccgtga cactgtcaca agcgcgagag ctggatgcgc tggcaaaaag
    14161 cctggggcgt gtgctgtctg tattccataa ccgccgttgg gatagcgatt tcttgacgct
    14221 aaaaggtttg ctcgcggaag gcgtgctggg tgaagttgct tactttgagt ctcattttga
    14281 ccgcttccgt ccgcaggtgc gcgatcgttg gcgtgaacag ggcggcccag gcagcggtat
    14341 ctggtacgat ttagcaccgc atcttcttga tcaggccatt acgctatttg gtttaccggt
    14401 cagcatgacg gtagatttgg cacagttacg gcccggagcg cagtcgaccg attatttcca
    14461 cgccatcttg tcctatccac agcggcgagt cattttacac ggtaccatgc tggcagccgc
    14521 tgagtcagca cggtatatcg tgcatggatc ccgaggcagt tatgtgaaat atggcctcga
    14581 tccacaggaa gaacgtctga aaaatggcga gcgtctgccg caggaagact ggggctacga
    14641 tatgcgtgat ggcgtactta cccgcgtgga aggtgaggaa cgtgtcgaag aaacgctgtt
    14701 gacggtgcct gggaattatc cggcttacta tgcggcgatt cgtgatgcgt taaatggcga
    14761 tggtgaaaat ccggttccgg caagccaggc aatccaggta atggagttga ttgagctggg
    14821 catcgaatcc gccaaacatc gcgcgacgtt gtgccttgcg tgaaaaatgg atggtgcaat
    14881 ttgcaccatc catttttgtt acttcgcggc gactttttct cgcagtgcgc gtttttcctc
    14941 tgcgctgagg aaagccattt ccagaccatt aatctgtgcc tggcggattt gctcacggga
    15001 taacccagca gctggcgcgg caacggtata ttcgtgaatg atatccactc cctgtacgcc
    15061 gggatcgtca gtgttaatgc tggcacgaat gccatgctca aggaacgttt tcagcggatg
    15121 tgcagccagc tctgctacgg tgctggtctg aatattggag gtcagacagg attcaatacc
    15181 aatttgttgc tcggcgagaa aatccatcag cgcccgatct tcaatggctt ttacgccatg
    15241 tccaatacgc tccgcaccca gttcacgaat cgcctgccag atgctttccg gcccggcagc
    15301 ttcgcctgca tggacggtaa tatgccagcc cgcatcacgc gcgcggttga agtgagaaag
    15361 gaacagactt cccgggaatc caagttcatc accagctaaa tcaagtgcgg taatctggtc
    15421 acggtgggct aaaaaggcct ccagctcttg ctgacaggcg gcttcgccga aggttcggct
    15481 cataatgccg ataagcttcg cctgcacacc aaaggtgcgg caaccttcac gtacgccatc
    15541 gatcaccgct tcgacaacac ccgctacagg cagcttatgt gccattgcca tgtagcctgg
    15601 tgaaaaacgc agctcgacat agcgcaggcc gttacgggct gcatcttcaa tgttttcaaa
    15661 tgccacgcgg cggcaggcat caagagaggc gagaacttta acgccccagt caagtttggt
    15721 cagaaagctc accagatcgg gttcgttggc aatgacctga acgtggggaa tcagtgtttc
    15781 aagggattgt gcaggaagcg agatattata ctggcggcca agttcaagaa tggtctgggg
    15841 acgaatgttg ccatcaaggt ggcgatggat atcagttaat ggcagggtgg tatcaatcat
    15901 ggtcgcactc tttttctgga tataaaagtg cgcaccatta taaaaacaaa gcagggtaaa
    15961 aatccacgat tgcgcaacga ttttttacgt tgcgcaattg gggttagcga acagcgcgga
    16021 tggcgtttat taatccagcc acaccttttt ccagtttcga acgtgggcag ccggcattga
    16081 gacggacaaa accacgacct tcttcaccat aggtataccc cggcatgatc gcgacttttt
    16141 cttgctcgat aagcgctttt tgcaacgcgt tgtcgtcaat attcaacgga cgtagatcaa
    16201 gccaggccag ataagtggat tgcgggatct gccagttgag ttcaggaaac gcggcgttca
    16261 ttttatctga gatatacgtc aggttatctt tcagatagac gcgtaaggca tccagccacg
    16321 gtgcaccttg ctgataggcg gcgatatggg cagttaacgc cagtaccgaa ggggaagaaa
    16381 gcccatcacg tcctttcagt gccgataaat aggcatcacg gctgctgcta ttttctataa
    16441 tcccgtaagc accggtcagc gcgggaatat tgaaactttt cgatcccgat gtcagcaacg
    16501 cccagtctcc ccgcgccaca ttactccagg gaatatgcgg ctgctcgccc cagaccatat
    16561 ccatatggat ttcatcggaa ataacccgca catcatgacg ctcgcacagg tcagccatga
    16621 tctccagctc atcgcacgtc cacactttcc cggtaggatt ctgtgggtta cacaggagca
    16681 taattttaca ttctggtttc gccaaaacgg cttccagctt gcccatatcg caaaaccagc
    16741 cgtcagcctg cttctctaaa gcaacgggca ttactgtgcg ctggttacct tcaatggcct
    16801 tgtaaaatgc gtcataggcg ggcgtgtgga tcaccacgcc ttcacctgtt tcagaccact
    16861 gacgaatcag ttctgaaacc atatagatga cagaagggcc atacaccacc gtctgcgtat
    16921 caattgcggt gtaatgctgg gtggaaaacc agtgggcaat agccgcgaga aactcatcgt
    16981 ttttccagcg gctgtagcca aatacgccgt gcatcaggcg ctgattcagc gcctcgataa
    17041 tgcagggggc agtggcaaaa tccatgtctg aaatcgtgaa cggtaacagg tcagcagtgc
    17101 cgaaacggtc agcgacataa tcccactgtg tacaccatgt gccatgacga tccacgacct
    17161 ttgaaaaatc gaacatatct tatccttatg cctggacagt atgcatcaga ccggccattt
    17221 catcttttac tgactgaact tgtggcccga taacaacctg caggttatgt tgattaagtt
    17281 gtactacgcc aattgcccga ttgtccttca gtgcctgcac attaacaagc gacatatctt
    17341 ccacagacaa acgcagacgg gtaatgcagt tatcgaggct gacaatattg tcggcaccgc
    17401 ctaatgcttc gaggattgca ggaacgttgt aacctgattt acccggcgca ccggcaacgg
    17461 ctttttcgat tgagctggca acttcgctat cgcgccccgg ggttttcaga ttgaagcggg
    17521 tgatagcgaa acggaagatg acgtagtaaa cgacaaacca gattgccgcc accactggca
    17581 ccatgtacca cttggttgac agaccatgca aaataccgaa caccacgaag tcgatgatat
    17641 tgccgtcggt attaccgatg gtgacgccga gcacagacat gacggtgaag ccgaggccgg
    17701 ttaacagcgc gtggatgaca tacagaactg gcgctacgaa caggaacagg aattccagcg
    17761 gttcggtagt gccgccaacg acgcaggcga tcaggccaga aatcagcaga cctttaattt
    17821 tatggcgatt ttccgggcgc gcgcagtgat acatagctaa cgctgcacct ggtagaccgc
    17881 cgagaaacgc aggcatttta ccttgcgaaa ggaaacgcgt gacgctttca gaaaaaccgt
    17941 gagtggtcgg gcaactcaat tgcgcctgga agatggtcag tgcgccgctg acggtttgac
    18001 cgcagacctc ctgcgtgccg cctgcgtcgg taaagcgaat taatgccacc agaatgtgat
    18061 gcagaccaaa cggcaacagc agacgttcac cggtaccaaa cagcatcggt ccgaaatcac
    18121 ccgcgctgtt tatcatatgg cccaagccgc taatacccat ggcgaaaatc ggccagacta
    18181 atggaatcac caggccgaca aggcccatca ccagcgagga gataattggt acgaagcgcg
    18241 taccgccgaa gaatgccagc gcatccggca ggcggatatt atggaaacgc tcatgcagca
    18301 tccagacgat aataccggcg atcaccgcac cgaggatccc ggtatcgatc gactggatcc
    18361 caagaatgct ctggatgtta ttggctttca gaacggcggc atctgtggtt ggcagaatgc
    18421 ctttattggt caaccagaag tttaccgcga ggttcattac cgcataaccg acgaagccag
    18481 cgaatgccgc tacgccttta ttttcgcgtg ccaggcccag cgggatggcg atacagaaca
    18541 tgacaggcag gaaactaaaa gcaaacgagc caatcttact catccaggta aagatagcct
    18601 gcaacacggg gttgcccagg accgggatca gggttatgac atcatggctg ctaagagaac
    18661 taccaatgcc gagcataatg ccgcagaacg acaataatgc cacgggtaac atgaaggttt
    18721 tgcctaactg ctggaagaac tcccacagcg tgactttcgg tgctgttttc gccgtcataa
    18781 aacgactcct cgtagagaac aatctggatt cagtaaattg cgcgagaaga taaaacgttt
    18841 tatcaaattt tagtgagtca taaatcacat tacgcaacga taatagcggg tataagataa
    18901 ataaaaggta aaacgtttta tctgtcacat aatcagggag taggtcatct gcatggctac
    18961 cgccaaaaaa ataaccattc atgatgttgc gctggctgcg ggcgtgtcgg taagtaccgt
    19021 ttcgctggtg cttaatggca aagggcgaat ctctaccgcc acaggagaac gcgttaacgc
    19081 cgccattgaa gagctgggat ttgtgcgcaa tcgccaggcg tcggcgctgc gcggcgggca
    19141 aagcggcgtc attggtttga tcgtccgtga tttatctgcg ccgttttacg ccgaattgac
    19201 ggccggattg acggaagctc tggaagcaca gggacggatg gtttttttgc ttcacggcgg
    19261 taaagacggc gagcagctgg cacagcggtt ttcactgtta ctgaatcagg gtgtcgatgg
    19321 tgtggtaatt gccggggctg caggaagcag cgatgacctg cggcggatgg cagaagaaaa
    19381 agctatcccg gtgattttcg cttcccgtgc cagttatctt gatgatgttg atacggttcg
    19441 cccggacaac atgcaggctg cacagttgtt gacggagcat ctcattcgca atgggcatca
    19501 gcggatcgcc tggctgggag ggcaaagttc ctcattaacc cgtgcagaac gggtgggggg
    19561 ctattgtgca actctactaa aatttggcct gccgtttcac agcgattggg tgttggagtg
    19621 cacttccagc cagaagcaag ccgcggaagc tatcacggcg cttttacgtc ataacccgac
    19681 catcagtgcc gtggtttgct ataacgaaac tattgcgatg ggggcatggt ttggtttgct
    19741 gaaagcaggg cggcaaagcg gggaaagcgg agtcgatcgt tactttgagc aacaggtttc
    19801 gctggcggca tttaccgatg cgacaccaac cacacttgat gatatacctg ttacctgggc
    19861 cagcacgcca gcgcgggaac ttggtatcac acttgcggat cgcatgatgc aaaaaatcac
    19921 ccatgaagag acgcattcac gcaatcttat tattcccgcc cggctcattg cggcgaaata
    19981 atcctctctt tatctgctat acttggtagt gtcctttcct caaggttaat gataaaaaat
    20041 gtggcacaca ggcggtgcct tttattttta ctgaagttca caggaggttt atgtgtttaa
    20101 ttctgacaac ctgagactcg acggaaaatg cgccatcatc acaggtgcgg gtgcaggtat
    20161 tggtaaagaa atcgccatta cattcgcgac agctggcgca tctgtggtgg tcagtgatat
    20221 taacgccgac gcagctaacc atgttgtaga cgaaattcaa caactggatg gtcaggcatt
    20281 tgcctggcgt tgtgatatta cttccgaaca ggaactctct gcactggcag actttgccgt
    20341 cagtaatctg ggtaaagttg atatcctggt taacaacgcc ggtggcggtg gtcctaaacc
    20401 gtttgatatg ccaatggcag attttcgccg tgcttatgaa ctgaatgtgt tttctttttt
    20461 ccatctgtca caacttgttg cgccagaaat ggaaaaaaat ggaggtggcg ttattctgac
    20521 catcacttct atggcggcag aaaataaaaa tataaacatg acttcctatg catcatctaa
    20581 agctgcggcc agtcatctgg tcagaaatat ggcatttgac ctgggtgaaa aaaatattcg
    20641 ggtgaatagc attgcgccgg gggcaatatt aaccgatgcc ctgaaatccg ttattacacc
    20701 a
//
LOCUS       DAZOZX010000065        20692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZOZX010000065 DAZOZX010000000
VERSION     DAZOZX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.065"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            82..351
                     /locus_tag="ACHUPT_002349"
     CDS             82..351
                     /locus_tag="ACHUPT_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR0819900.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="ACHUPT_002350"
     CDS             345..710
                     /locus_tag="ACHUPT_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0819901.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="ACHUPT_002351"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="ACHUPT_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0819902.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="ACHUPT_002352"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="ACHUPT_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0819903.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="ACHUPT_002353"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="ACHUPT_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0819904.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="ACHUPT_002354"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="ACHUPT_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0819905.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="ACHUPT_002355"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="ACHUPT_002355"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0819906.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="ACHUPT_002356"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="ACHUPT_002356"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0819907.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="ACHUPT_002357"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="ACHUPT_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="ACHUPT_002358"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="ACHUPT_002358"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0819908.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="ACHUPT_002359"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="ACHUPT_002359"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0819909.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14850..16448
                     /gene="rtcR"
                     /locus_tag="ACHUPT_002360"
     CDS             14850..16448
                     /gene="rtcR"
                     /locus_tag="ACHUPT_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR0819910.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16430..17188)
                     /locus_tag="ACHUPT_002361"
     CDS             complement(16430..17188)
                     /locus_tag="ACHUPT_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0819911.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="ACHUPT_002362"
     CDS             complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="ACHUPT_002362"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0819912.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="ACHUPT_002363"
     CDS             complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="ACHUPT_002363"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0819913.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18596..20101
                     /gene="glpD"
                     /locus_tag="ACHUPT_002364"
     CDS             18596..20101
                     /gene="glpD"
                     /locus_tag="ACHUPT_002364"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0819914.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANALMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20307..20588)
                     /locus_tag="ACHUPT_002365"
     CDS             complement(20307..20588)
                     /locus_tag="ACHUPT_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819915.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4889 a         5692 c         5217 g         4894 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggt tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaatttat tgtttctaaa
    14761 aagattatat ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg
    14821 aaatatcgcc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    14881 gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac
    14941 tctgtatata ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc
    15001 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag
    15061 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    15121 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    15181 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt
    15241 atctacccgc ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc
    15301 caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    15361 ttgccgagga acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc
    15421 cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga
    15481 ttctgcttaa cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt
    15541 taaaacaggc acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    15601 gcggcgatac cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    15661 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    15721 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct
    15781 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    15841 tgcgcgattt gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga
    15901 tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga
    15961 acctggatta tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca
    16021 ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta
    16081 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    16141 tcactctgga cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc
    16201 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    16261 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    16321 gacgacagct ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    16381 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    16441 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg
    16501 tgtagacggc atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt
    16561 ttgaatggtc aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac
    16621 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca
    16681 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    16741 cgccatcgcg gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca
    16801 tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca
    16861 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    16921 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    16981 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca
    17041 ggatcaggtt ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg
    17101 agaagtgctc caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    17161 taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc
    17221 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct
    17281 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    17341 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    17401 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    17461 aatttttgct gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    17521 ccgctaccga ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag
    17581 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    17641 ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt
    17701 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    17761 cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    17821 cctgccagcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    17881 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    17941 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    18001 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    18061 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc
    18121 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    18181 gcgccgcccc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    18241 cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    18301 gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    18361 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    18421 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    18481 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    18541 taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga
    18601 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    18661 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    18721 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    18781 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc
    18841 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    18901 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    18961 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    19021 tgactgttgg gtagacgacg cccgtctggt actcgccaac gccctgatgg tggtgcgtaa
    19081 aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg
    19141 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    19201 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc
    19261 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca
    19321 aaagcaggcc tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat
    19381 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt
    19441 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    19501 gcagctaagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    19561 tgagtccgac tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa
    19621 cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    19681 agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa
    19741 agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct
    19801 gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg
    19861 cagcaacagc gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt
    19921 cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg
    19981 caccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    20041 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta
    20101 aattaacgta aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa
    20161 aactgcctga tgcgctacgc ttatcaggcc tacgtggttt atgcaatata ttgaatttgc
    20221 atggtcttgt aggccagata agacgttcac gtcgcatccg gcatgaacaa agcgcacttt
    20281 gtcaaaaatc taacctactt tttaattcag gaaattaccg caaagcccac gccatcatac
    20341 ccaacgtaac aaaactcagg cacgttcccc actcgccccg agaaaaatag cattaatgcg
    20401 cccagcgtca gcataaaaat attgagcaga aggtaaattg ttggcgtaaa gagtaaattg
    20461 caaacctcag gaatagcggc aagcccagcc cccagcatgg caataaccgc tgcaaaaaat
    20521 tgcgtcagta ttgggatgcc gaaaaaagtc attaccagcg aggtcaaaat ccattttcta
    20581 ttttgcatta ttctttccat tctttctgaa tggcgaaatt atactccccg agtccccttg
    20641 ccccttctgg acacttttcc gaaatgatgg cgaaaaaaac gggacccttt gg
//
LOCUS       DAZOZX010000066        20661 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZOZX010000066 DAZOZX010000000
VERSION     DAZOZX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.066"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..92)
                     /locus_tag="ACHUPT_002366"
     CDS             complement(<1..92)
                     /locus_tag="ACHUPT_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819916.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKT"
     gene            complement(270..1919)
                     /gene="actP"
                     /locus_tag="ACHUPT_002367"
     CDS             complement(270..1919)
                     /gene="actP"
                     /locus_tag="ACHUPT_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0819917.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1916..2230)
                     /locus_tag="ACHUPT_002368"
     CDS             complement(1916..2230)
                     /locus_tag="ACHUPT_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR0819918.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2430..4388)
                     /gene="acs"
                     /locus_tag="ACHUPT_002369"
                     /pseudo
     CDS             complement(2430..4388)
                     /gene="acs"
                     /locus_tag="ACHUPT_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4781..6217
                     /gene="nrfA"
                     /locus_tag="ACHUPT_002370"
     CDS             4781..6217
                     /gene="nrfA"
                     /locus_tag="ACHUPT_002370"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0819919.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6262..6828
                     /gene="nrfB"
                     /locus_tag="ACHUPT_002371"
     CDS             6262..6828
                     /gene="nrfB"
                     /locus_tag="ACHUPT_002371"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0819920.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6825..7496
                     /gene="nrfC"
                     /locus_tag="ACHUPT_002372"
     CDS             6825..7496
                     /gene="nrfC"
                     /locus_tag="ACHUPT_002372"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0819921.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7493..8449
                     /gene="nrfD"
                     /locus_tag="ACHUPT_002373"
     CDS             7493..8449
                     /gene="nrfD"
                     /locus_tag="ACHUPT_002373"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0819922.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8469..10187
                     /locus_tag="ACHUPT_002374"
     CDS             8469..10187
                     /locus_tag="ACHUPT_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0819923.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQENSLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10180..10563
                     /gene="nrfF"
                     /locus_tag="ACHUPT_002375"
     CDS             10180..10563
                     /gene="nrfF"
                     /locus_tag="ACHUPT_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0819924.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10560..11156
                     /gene="nrfG"
                     /locus_tag="ACHUPT_002376"
     CDS             10560..11156
                     /gene="nrfG"
                     /locus_tag="ACHUPT_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0819925.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11498..12811
                     /gene="gltP"
                     /locus_tag="ACHUPT_002377"
     CDS             11498..12811
                     /gene="gltP"
                     /locus_tag="ACHUPT_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0819926.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12889..13578)
                     /gene="yjcO"
                     /locus_tag="ACHUPT_002378"
     CDS             complement(12889..13578)
                     /gene="yjcO"
                     /locus_tag="ACHUPT_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0819927.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13672..15819)
                     /gene="fdhF"
                     /locus_tag="ACHUPT_002379"
                     /pseudo
     CDS             complement(13672..15819)
                     /gene="fdhF"
                     /locus_tag="ACHUPT_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(16017..17483)
                     /gene="mdtP"
                     /locus_tag="ACHUPT_002380"
     CDS             complement(16017..17483)
                     /gene="mdtP"
                     /locus_tag="ACHUPT_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR0819928.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17480..19522)
                     /gene="mdtO"
                     /locus_tag="ACHUPT_002381"
     CDS             complement(17480..19522)
                     /gene="mdtO"
                     /locus_tag="ACHUPT_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0819929.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19522..20553)
                     /gene="mdtN"
                     /locus_tag="ACHUPT_002382"
     CDS             complement(19522..20553)
                     /gene="mdtN"
                     /locus_tag="ACHUPT_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0819930.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20572..>20661)
                     /locus_tag="ACHUPT_002383"
     CDS             complement(20572..>20661)
                     /locus_tag="ACHUPT_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819931.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4637 a         5455 c         5659 g         4910 t
ORIGIN      
        1 gttttctttg tatgtgatga acttgtgata gagtggttat cgttaatctg gcaaagaggg
       61 aaaaacatat tatttaaacc attatagcgc ataaaatatc cttataatta acaagaaaga
      121 aaaggcatat tctctgcatt atcattttct acgactgtca aaaatcgctc attttttaat
      181 gagtttattt gtttaatatt atgggaaaag gtgatgcatg ttggagagga agcgtattcc
      241 ccggtcaggc gaccggggaa ggggagagat taatgtgcgc ggccttgctc aacgccaaag
      301 ccggtctggg agcggataaa ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct
      361 gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg ccacgctgat agagaacagc
      421 gccgggtatt catacgggaa gatggctttt tcgtgaccga ggatctgtac ccaaatagtc
      481 gggccgagga tcatcagcac cactgcggta atcagcccca gccagccgcc cagcatcgcg
      541 ccacgggtag tcagtttcga ccagtacatg gaaagcagaa tgatcgggaa gttacagctc
      601 gccgcgatgg caaacgcaag gcctaccata aaggcgatgt tctgattctc aaacagcacg
      661 ccgaggataa tcgcaatcac gccgaggatc agtacggtga tttttgatac ccgcagctct
      721 tcacgttcgg tcgcgccttt tttgaagacg ttagcgtaca agtcatgcga aaccgccgat
      781 gcgcccgcca gcgtcagatc cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa
      841 ataaaaccga ggaataggtt gccgcccact gcattcgcca ggtgaacggc cgccatgttg
      901 ttaccaccaa tcagatggcc cgccgcgtct ttatattccg gattcgcacc aacaagcatg
      961 atcgcgccga agccgataat aaaggtcaga atatagaagt agcccataaa tccggtggcg
     1021 tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg taaagaagcg catcagaatg
     1081 tgcggcaagc ccgccgtacc aaacatcagt cccagcccca gagagagcgc ggagatcgga
     1141 tctttcacca gtccgcccgg tttcatgatg tcgacacctt tcgggtgtac cgccatcgct
     1201 tcgctgaaca gattgttgaa gctaaagccg acgtgtttca tcaccataaa ggccataaag
     1261 ctggcaccga acagcaacag cacagctttg ataatttgta cccaggtggt cgccagcatg
     1321 ccgccgaaca ggacgtacat catcatcagc acgccgacca gcaccaccgc aatgtgatag
     1381 ttaaggccaa acagcagctc gatcagttta cctgcgccta ccatctgagc gataaggtaa
     1441 agcgccacca ctaccagaga accacaggcc gaaagaatac gaatcggccc ttgtttcaga
     1501 cgataagagg ccacatcggc agaggtgtag cgtcccaggt tacgcagacg ttcggcaatc
     1561 agaaacagaa tgattggcca gcccaccagg aagcccaacg agtagatcag cccgtcatag
     1621 ccggaggtaa acaccagcgc ggagatcccc aagaatgagg cggcggacat atagtccccg
     1681 gcaatcgcca gcccgttctg gaagccagtg atattgccgc ctgcggtgta gtagtcgcta
     1741 cgagaacgta cgcgttttga tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc
     1801 aggaacataa taatcgcctg ccagttcgtt ggctggcgct ctacggcccc gctaatagca
     1861 tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg
     1921 atgcttgtac ctcatgcagg acttcattat taagacggtc gaattcgccg ttcgcccgcc
     1981 agatgtagat accggtgaga acaaaggaga tcacaatcac tccaacacca attggaatac
     2041 cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag ccagccgggc gcgaaggcga
     2101 tcagtaaaat aaagccgata taaactgcca gcataataat cgacaggatg gtggcaaacc
     2161 gttgccgttt ttcgactaac tccctgaaat gcgcattgtc ttctatccgc tgataaatcg
     2221 taccattcat cacagattct ccagaggtaa tgtagggatt gttttaattc ccgctccctt
     2281 atgggagaag gttaacgctc gggtaaccct tgccgaatgt aggccggata aggcgtttac
     2341 gccgcatccg gcaatcaatg cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc
     2401 gttaccgatt cgcatccggc aattgtgggt tacgatggca tcgcgatagc ctgcttctct
     2461 tcaagcagct tctcgaccac gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg
     2521 gtatcgcccg ccgcaatttt gcgcagaata cggcgcataa ttttgccgga gcgggtttta
     2581 ggcagggagt cggtccagtg cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc
     2641 acccagttgc ggacttctgc gtacagttct ggtgacggtt cctccccgtg attaagcgtg
     2701 acataggcgt agatcgcctg acctttaata ttgtgcggaa tacctactac ggcggcttcg
     2761 gcaatcttcg gatgcgccac cagcgccgac tctatctctg ccgtccccag acggtgaccg
     2821 gagacgttca gcacgtcgtc cacacgcccg gtgatccagt aatagccatc ttcatcgcga
     2881 cgcgcgccgt cgccgctgaa atacatattt ttgaaggtgg aaaaataggt ctgctcaaaa
     2941 cgttcgtgat cgccaaacag cgtacgcgcc tgaccaggcc aggagtcggt gatcaccagg
     3001 ctaccttcgg tagccccttc cagcgggtta ccttcgttat cgaccagcgc cggttgcacg
     3061 ccgaagaacg gacgtgttgc cgaaccggct ttcagctcgg tagcgccagg cagcggggtg
     3121 atcatgaaac cgccggtttc ggtctgccac caggtatcga ccaccggaca tttctcgttg
     3181 ccgatttttt tccagtacca ctcccacgct tccgggttaa ttggctcgcc cacggaaccg
     3241 agaatgcgta gcgacgaacg gtcggtgcct tcaatcgctt tatcgccttc cgccatcagc
     3301 gcgcggatcg ccgtgggtgc ggtatagaga atattgacct gatgcttgtc caccacctgc
     3361 gccatacggg caggcgtcgg ccagttcggt acgccttcaa acatcagcgt ggttgcgccg
     3421 caggccagcg ggccgtacag caaataactg tgtccggtaa cccagcccac atcggcggtg
     3481 caccagtaga tatcacccgg atgataatca aagacatatt taaaggtcag cgccgcgtac
     3541 accagataac cgccggtagt gtgcagtacg ccttttggtt ttccggtaga accggaggta
     3601 tagagaataa acagcggatc ttcggcgttc atctcttccg cctagtgctg atcgctggct
     3661 tgctcaacca ggtcgtgcca ccacaggtcg cgcccttcct gccagtcgat gtttccgcca
     3721 gtacgcttca gtaccaccac atgctctacg ctggtgacgt tcgggttttt tagtgcgtca
     3781 tcaacgtttt tcttcagcgg aatactacgc ccggcgcgca cgccttcgtc ggaagtgatc
     3841 accagtcgtg agttggaatc aataatgcgc ccggcaacgg cttccggcga gaagccgccg
     3901 aaaatcaccg aatgcatcgc gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc
     3961 ggcaccatcg gcatataaat cgccaccaca tcaccttttt taatgcccag ctcgagcagg
     4021 gtattggcga agcggcagac gtcgcggtgc agctctttat agctgatatg tttgctctgg
     4081 ctggcgtcgt cgccttccca gatgatggcg gtgcgatcgc cgttttcttg cagatggcgg
     4141 tcaaggcagt ttgccgccag attcagcgtg ccgtcctcgt accatttaat ggacacatta
     4201 ccgggggcaa aggaggtatt tttcaccttc tggtacggtt tgatccagtc gagaattttt
     4261 ccctgttcgc cccagaaggt atcaggtacg ttaatagatt gttgatacat cgcctcgtac
     4321 tgctgagggt ttatcaggca acggtctgcg atgttggcag gaatggtgtg tttgtgaatt
     4381 tggctcatgc ttttgttctc cttgtaggat gttaataata tgtggcataa gcgttaaatg
     4441 taggggtatt ggcagttttg tttagtattt gggcgacaga tcacgcaaaa gtagaatggt
     4501 gcaaataaac ggcagggtaa tttttgaagg tcaccaacaa aagttgatta attctttgag
     4561 gaacatgcag ttatgcatgc tgttgaaaaa gaggaagata ctgactaact ctaaagtggt
     4621 atttcacatg cacttacaat tgattaaaga caacattcac agtgtggtta tttgttacac
     4681 ataggggtga gcaatgtcat gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa
     4741 accgccattg caacaatggc gcaattcgga tgaagcccct atgacaagga taaaaataaa
     4801 cgcacgccgt atcttcagct tattgattcc ttttttcttt ttcacttctg ttcacgctga
     4861 acaaacggca gctcccgcaa aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc
     4921 gcagcatcac gatcaatatc tctcctggaa agccacctcg gaacagtcag agcgtgttga
     4981 cgccctggcg gaagatccac ggctggtgat cctgtgggcg gggtatccct tctcgcgcga
     5041 ttacaacaag cctcgtggac atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac
     5101 cggtgcgccg aaaaacgctg aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag
     5161 cccggatgtg gcgcgtctga tccagaaaga cggcgaagat ggctacttcc acggtaaatg
     5221 ggcgcgcggc ggcccggaaa tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc
     5281 ctcaccagag ttcgccaaag gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg
     5341 cgcgatggaa gccattggta aaccttttga gaaagccgga cgtttcgacc agcaatcgat
     5401 ggtttgcggt cagtgccatg tggagtatta cttcgacggc aaaaacaaag cggttaaatt
     5461 cccgtgggat gacggcatga aagtcgaaaa tatggagcag tattacgaca aaattgcctt
     5521 ctctgactgg actaactccc tgtcgaaaac gccaatgctg aaagcgcagc acccggaata
     5581 tgaaacctgg acagcgggca ttcacggtaa aaacaacgtg acctgtatcg actgccatat
     5641 gccaaaagtg cagaacgccg aaggcaaact ctacaccgac cataaaattg gtaatccgtt
     5701 tgataacttc gcccagactt gtgcgaactg ccatacccag gataaagctg ccttgcaaaa
     5761 agtggtcgcg gaacgtaagc aatcgattaa cgacctgaaa atcaaggttg aagatcaact
     5821 ggttcacgct cacttcgaag cgaaagcggc gctggatgca ggcgcgacgg aagccgaaat
     5881 gaagccgatt caggacgata tccgtcatgc ccagtggcgt tgggatctgg cgattgcttc
     5941 ccacggtatt catatgcacg caccggaaga aggtctacgg atgctcggta cggcgatgga
     6001 taaagcggcg gatgcgcgca ccaaactggc gcgcctgctg gcgaccaaag gtatcaccca
     6061 tgaaatccag atcccggata tctcgaccaa agagaaagcc cagcaggcca ttggcctgaa
     6121 catggaacaa atcaaggccg agaagcagga cttcatcaaa acggtgatcc cgcagtggga
     6181 agagcaggca cgtaaaaacg gtctgttaag ccaataaccc cgttccgcct cgcaaggggc
     6241 ggaaaacaca atggagtgaa tatgagcgta ttacgttcgt tattaactgc tggggtgctg
     6301 gcgtcgggcc tgttgtggag cctgaacgga attaccgcta cccctgcggt gcaggcatct
     6361 ggtgatcgct acgaagttac ccagcagcgt aacccggatg ccgcctgtct ggactgtcat
     6421 aaaccagata ccgaaggtat gcatggcaaa catgcttccg ttatcaaccc gaataacaaa
     6481 ttgccggtca cctgcaccaa ctgtcacggc caagcatcac cgcaacaccg cgaaggggtg
     6541 aaagatgtga tgcgctttaa cgagccgatg tataaggtcg gagagcagaa cagcgtctgt
     6601 atgtcctgtc atctgccgga acagttgcaa aaagcgttct ggccgcacga tgtccacgta
     6661 accaaagtgg cgtgcgccag ctgccattcc ctgcatccac agcaagatac gatgcagacg
     6721 ttaagtgaca aaggacggat taagatttgc gtcgattgcc acagcgatca gcgcaccaat
     6781 ccgaacttta acccggcgtc tgttccgttg cttaaggagc agccatgacc tggtctcgtc
     6841 gccagtttct caccggcgtc ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg
     6901 tggcgaaaac gttgaatatc aatggggtgc gttacggcat ggtgcatgac gagtcgttat
     6961 gcatcggctg taccgcctgt atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg
     7021 tctcgcgcct gacgattatt cgtagcgagc cgcagggcga atttcctgat gtgaaatatc
     7081 gcttcttccg taagtcttgc cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga
     7141 ccggtgcgtc ttttcgtgat gccgctagcg ggattgtcga tgttaatcct gatctctgcg
     7201 tcggttgtca gtactgcatc gccgcctgtc cgtaccgcgt gcgctttatc catccggtca
     7261 cgaagacggc ggacaaatgc gatttctgtc gtaagaccaa tttgcaggcc ggtaagcttc
     7321 ccgcgtgcgt tgaagcttgc ccgacgaagg cgctgacgtt tggcaatctg gacgatccta
     7381 acagtgagat ttcgcaactg ctgcgccaga agcctactta ccgctacaag ctggcgctgg
     7441 gaaccaaacc gaagctgtac cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca
     7501 gacttccgca tttcattttg aatcgctggt gtgggactgg ccgattgcca tctacctgtt
     7561 tttgattggt atttctgccg gtctggtgac gctggccgtg ctgttacgtc gcttctaccc
     7621 gcaggcgggc ggtgcagaca gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc
     7681 ggtgatcctc ggtctgttga tcctcgtctt ccacctgaca agaccgtgga ccttctggaa
     7741 gctgatgttc cactacagtt ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct
     7801 ctacatggtg gtgctggtgc tgtggctggc gaaaatcttt gaacatgatt tgcttgccct
     7861 gcaacaacgc tggttgccga agctggggat cgtgcaaaag gttctgagcc tgctgacgcc
     7921 cgttcatcgc ggactggaaa cgctgatgct ggtgttggcg gtgctgctgg gggcttatac
     7981 cggctttctg ctgtcggcgc tgaaatcgta tccgttcctc aataacccga gcctgccggt
     8041 gctgctcctc ttctccggca tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat
     8101 acgccaacgc agtaacccgc attccacgga agcgcacttt gttcaccgga tggaaatccc
     8161 cgtggtatgg ggtgaaatct tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga
     8221 tgacggtaaa gttcgtgcgc tggtggcggc attaggtggc ggtttctgga cgtggtggtt
     8281 ctggcttggt gtcgccgggc tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa
     8341 tcgcagttcc ggcattcctg ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt
     8401 gatgctgcgc tttttcattc tctacgccgg acagttgacg gtggcgtaag ccagaaaaga
     8461 ggtggtttct ggacgtattc cttcctgaag tcggttttct ggcgttgttg ttaagtctcg
     8521 gggtcaacgt gttgaccccg ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca
     8581 tgatgcgact cacttgcatc ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg
     8641 gcgtactgac gtattgtttt ctcatcagcg atttctcggt catttatgtc gcccaacata
     8701 gctacagcct gctgtcgtgg gaactcaaac tggcggcggt gtggggcggt catgaaggtt
     8761 cgctgctgct ttgggtgctg ctgctttccg cctggagcgc gctgtttgcc tggcattatc
     8821 ggcagcaaag cgatccgcta tttccgctga cgctagccgt tttatctctc atgctcgccg
     8881 cactgctact gtttgtggtg ctgtggtccg atcccttcgt gcggatattt ccaccagcaa
     8941 tcgaaggccg cgatctcaat ccgatgctgc aacatcccgg tcttatcttt catccaccgc
     9001 tgctttacct tggctatggc ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac
     9061 tgcgcggcga gtttgatgcg gccagcgccc gaatttgctg gcgctgggcg ttacctggct
     9121 ggagtgcatt aacggcgggg atcatcctcg gttcctggtg ggcctactgc gaactcggct
     9181 ggggcggctg gtggttctgg gatccggtgg aaaacgcctc tttattaccc tggctttctg
     9241 ccactgcgct gctgcacagt ttgtccctga cacgccagcg ggggattttc cgccactggt
     9301 cgctgttgct ggcgatagtt actctgatgc tgtcgctgct gggcacctta attgtccgtt
     9361 ctggcattct ggtttcggtt catgcgttcg cactggataa cgtccgcgcc gtgccgttgt
     9421 tcagcctgtt tgcactgatt agccttgcgt ctttggctct gtatggctgg cgagcgcggg
     9481 acggtggccc ggcggtgcgt ttttcggggt tatcgcggga aatgttaatc ctcgctacgc
     9541 tgttgctgtt ttgcgcagtg ctactgatcg tgctggtggg aacgctttat ccgatgattt
     9601 acggcctgct gggctgggga cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt
     9661 taccgtttgg tctgttgatg ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac
     9721 gcgtgcagct tccggcgctg gtagctcatg ctggcgtgct gttatttgcc gcggggatcg
     9781 tggtttccag tgtcagtcgt caggagaaca gcctgaattt acagccgggt cagcaggtga
     9841 cgctggcagg atacaccttc cgttttgagc gactcgatct gcaagccaga ggcaattaca
     9901 ccagcgaaaa agcgatagtg gcgctgtttg accatcagca acgcattggt gaattaacgc
     9961 cggagcggcg tttttatgaa gcacgccgtc agcaaatgat ggaaccgtca attcgctgga
    10021 acggcatcca tgactggtat gcggtcatgg gtgaaaaaac cagagcggat cgttacgctt
    10081 ttcgtttgta tgtacaaagc ggtgtgcgct ggatctgggg gggaggattg ttgatgattg
    10141 cgggcgcatt gttaagcgga tggcggggga agaagcgcga tgaataaagg gcttctcacg
    10201 ctactgctgt tatttacctg ttttgcgcgc gctcaggttg tagacacctg gcaattcgcc
    10261 aatccgcaac aacagcaaca ggcgttaaat attgccagcc agttacgttg tccgcagtgc
    10321 cagaatcaaa acttactcga atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt
    10381 tatagcatgg tgtcggaggg gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc
    10441 tacggagatt ttgttcgcta taacccaccg ttaacgggtc agacgctggt gttgtgggcg
    10501 ctgccagtgg tgttgttatt gctgatggca ctgatcctct ggcgagtgag ggctaagcga
    10561 tgaaacagcc tcaaataccg gtgaaaatgc tgacaacact cacgatattg atggtattcc
    10621 tctgtgtcgg cagttatctg ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc
    10681 gtcagcgcga tccgctacat cagtttgcca gccagcaaac cccggaagcg cagcttcagg
    10741 cattgcagga taaaatccgc gctaatccac aaaacagcga acaatgggcg ttactgggcg
    10801 agtactatct gtggcaaaac gattacagca attcgctgct ggcgtatcgt caggcgttgc
    10861 aactacgtgg tgagaacgct gaactgtatg cggcgctggc gacggtgctt tattaccagg
    10921 ccagccagca tatgaccgcc cagactcgcg caatgatcga caaagccctc gcgctggact
    10981 ctaacgaaat caccgctctg atgctgctgg cttccgatgc gtttatgcag gcgaattacg
    11041 cgcaagccat cgaactatgg caaaaagtga tggatctcaa ctcaccccgg atcaaccgga
    11101 cacagctggt cgagtcgatt aatatggcaa aactgttgca gcggagatcg gattaatcat
    11161 ctggtgatga agattatgtc attgagctgc ataaaaaata atcgaatgaa catatgccaa
    11221 aaataatcac caatcagtat tattgcagat taaacaaata aaaatctttc cataacaaat
    11281 ggttattcat taatcctgcc ataaactcgc gttattatgc attaatgcag cgaaaagctc
    11341 tgttgttaaa gggttgcgca acataccgcg caaatgatac tgatcataag cgttaaaaaa
    11401 atctacaaac caacgcaaca caattcatgc cctggcagta tgtcacgttc tcgcgtttct
    11461 gaacggggaa cggcgctcca ttgaggaagt tattctgatg aaaaatataa aattcagcct
    11521 ggcctggcag attctgtttg ctatggtgct gggcattctc ctgggaagct acctgcacta
    11581 ccatagcgac agccgcgact ggctggtcgt caatttgctc tctccggcgg gtgatatctt
    11641 catccatctg atcaaaatga ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat
    11701 cgcgggtgtt ggtgatgcca aacagctcgg gcgtattggc gcgaaaacca ttatttactt
    11761 cgaggtgatc accaccgtcg ccatcatttt ggggatcact ctggcgaacg tcttccagcc
    11821 cggtgccggg gtggatatgt cgcagctggc gaccgtcgat atctcgaaat atcagagcac
    11881 tacggaagcg gtacaaagca gttcccacgg cattatgggc acgattttgt cgctggtgcc
    11941 gacgaacatt gtggcgtcga tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt
    12001 gctgtttggt ctggggcttt cttccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt
    12061 gttccgctcc atctctgaaa ccatgtttaa agtgactcac atggtgatgc gttatgcacc
    12121 ggtgggtgtg tttgcgctga ttgcggtgac ggtggctaac tttggtttct cgtctctgtg
    12181 gccactggcg aaactggtgc tgctggtgca tttcgccatt ctgttcttcg cgctggtagt
    12241 gctgggaatt gtggcacgtc tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa
    12301 agatgagctg attctggcgt actccactgc cagctctgaa agcgtactgc cgcgaattat
    12361 tgagaagatg gaagcctacg gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg
    12421 ttactctttt aaccttgatg gttcgacgct gtatcaaagt attgccgcta ttttcatcgc
    12481 tcagctgtat ggcattgacc tgtccatctg gcaggaaatc attctggtgc tgacgctgat
    12541 ggtgacctcg aaagggattg ctggcgtggc tggcgtgtcg tttgtggtgt tgctggcaac
    12601 gctgggtagc gtaggtattc cgctggaagg tctggcgttt attgctggtg ttgaccgtat
    12661 cctcgacatg gcgcgtactg cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat
    12721 tgccaagtgg gaacacaaat ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct
    12781 gggcaaattt gataaaactg cggatcaata attgaagatt gccggggata tcccccggca
    12841 atgtgtggat gcctgatgcg acgcttgccg cgtcttatca ggcctacctt aaccgttggt
    12901 taatttttca aactcttcac acccggtatc aaacccttcc atacagctca ggttcaacca
    12961 gtgcagcgcc ttttgtttgt tcttctcgat aaaaccttcc tcaccgttta agaacatcat
    13021 tcccgcccag tactcggaat aaccggttcg ggaaattgca gagctgcgtt tgaaatacca
    13081 ggttgccttg tcatcgtcgg ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag
    13141 catttgggca tcgacggcgg agtcattctc cagatcttcc gaggcgtttt ctaacagcga
    13201 aatcgccttt ggataatccg gtttacccgc ttgagtattt accagaatat gcgccagcgt
    13261 tacttcacct tctttactgc ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg
    13321 atagtccaga ctgaccggat tggtgatttt gatctgcgcc aacagcgcgc aggcatcagc
    13381 atcaccgctg tcggcggctt tctgtgccca atattcggct ttgctcaaat cgccggagct
    13441 aaaccagctg tcggcaagaa aatattgtgc acgtcggtcc ccggcctctg ctgcctttaa
    13501 atactggctg ccaggctcgg agtcgttcgc gtgggcaaag aatgtcaaaa acaacatcaa
    13561 tgcgataatt tttttcattt tgataatacg attgggtagg gagtaaccag tataaagagt
    13621 tcgaatggat aaaaaaacag cctccgaaag gaggctgtag aaaggactgt attacgccag
    13681 tgccgcttcg cgcaggcgag ttttcaactt gttgtactcg tcaatcacgt actgctcggc
    13741 ggcgcgctga tcggagatcg gctcgacgcg cacggcgcag tatttgtatt ccggcgtttt
    13801 cgtaatcggg cttaagcttt cggtaaccag ctcgttacag gcaccaatcc accactggta
    13861 ggtcatgtaa atcgcccctt tgttcggacg atcgctgacc tgcgcacggg tgataatttt
    13921 gcctttacgc gagtgcactc aaaccaatgc ctcatcttca atacccagac gtttggcgtc
    13981 ttcggtattg atttgtgcgt agccaggttc atcagccagc gccgccagtg ccgcacagtt
    14041 accggtcatc gaacggcaag agtagtgacc aacttcacgc accgttgaca gtaccatcgg
    14101 gtactcgtcg gtgagtttgt cgattggcgc tacccagtcg caggtgaaga actgcgccag
    14161 accgttcggg gtatcaaact tctctttaaa cagataagaa gtcccctgat cggcatctga
    14221 agtatcgcgg caaggccact gaatgaagcc cagttcgccc attttctcgt aagtcgcacc
    14281 gtagaaatcc gggcacagat gacgcaactc atcccagatc tcctgggtgt tgttgtagtg
    14341 catcggataa cccatacggg tggcgatttc actgatgatt tgccagtccg ttttcagatc
    14401 ccatttcggt tcaactgctt tgaagaaacg ctggaagcca cggtcagccg cagtaaacac
    14461 gccttcatgc tcgccccacg acgttgacgg caaaatgaca tccgccgccg acgcggtttt
    14521 ggttataaag atgtcctgaa cgatgaccag ttccagatct tcaaaggctt tacgtactgc
    14581 cgacagctcc gcatcagttt gcagcggatc ttcgcccata atgtacgcgg cacgcacttc
    14641 gccatgcgct gcgcggtgcg gcagctcgct gatgcgatag ccggtatgag caggcaggct
    14701 ttccacgccc caggctttgg cgaatttttc gcggttagcc ggatctttca cgtactggta
    14761 tcccggatac gtatccggca gcgcgcccat atcgcaggca ccctgcacgt tgttctgacc
    14821 acgaaccggg tttacacccg catgcggctt accgaggtta ctggtcagca tcgcgaggct
    14881 ggtcagagaa cgcacggttt ccacgccctg gtagaactgg gttacaccca tgccccacag
    14941 gatggcggcg cttttcgcct gggcatacat ccgtgccgcc tgacgaatct cactggcgct
    15001 gacaccggtg atatcttcaa ccgactccgg cgtgtagcct tcaacgattt tacgatactc
    15061 ttcaaagcct tctgtacggg aagcgacgaa cgctttgtcg tacagatttt cttcaataat
    15121 gacatggccc atcgcattca acagcgcgat gttcgagccg tttttcagtg caatgtgcat
    15181 gtcagcaatg cgcgcggttt caattttgcg cggatcgcag acgataattt tcgccccgtt
    15241 acgtttagcg ttaattacgt gattcgccac gattgggtgg gaatccgccg ggttgtaccc
    15301 gaaaacgaac actaaatcgg tattatcaat ttcgttaata gcattgctca ttgcgccatt
    15361 accgaccgat tggtgcagac ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc
    15421 aacgttattg gtaccaataa cggcgcgcgc aaatttttgc attacatagt tggtttcgtt
    15481 acccgtacca cgcgaggagc cggtcgtctg gatggcatcc ggaccgtact tctctttgat
    15541 ggcgctcagg cgctcggcaa cgtaattcag tgcctcatct caggaaacag gttcgagttt
    15601 gccgccacgc tggcgacgga tcatgggggt tttcaggcgc ggggtcagga tctgggtatc
    15661 gttaatgaaa tcccagccat aataaccctt cagacacagg gtaccctggt tggttttccc
    15721 ctgcgctgcc tccgcccgga cgattttgcc gttatcgacg accaggttga ttttgcaacc
    15781 tgatgcgcaa taggggcaaa ccgtgacgac ttttttcatc ggtctcgctc cagttaatca
    15841 aatcacgcat acgcgctctc gactacagta tgcatctttt atgccacatt ttatttgggg
    15901 tcattccctg atattacggg cactatttat tcaaaactct gacgaaaaac aggctgtcgt
    15961 cagttttgac gtgacgaaac gaaataccgc gtgacagcca tcacgcggca gacattttat
    16021 tttttctcga cgacgggagc cgcctgatac ccgccgccca gcgatttcat caactgaatg
    16081 ctttggatca cccggcggct gtccagcatc agtaatgaca tctcttcggc aagtactggc
    16141 aaccgggctt cagtggcctg taagcggctg gttaagccgc gctgataggc ggcctcggca
    16201 gcgcgctggg tgaagcgcgt tgcttccacg tgttcagcct gcatttctcg ctcgtcgttg
    16261 agcgtttgca gacgcgtgcc gttgacggca acgtcacgca ccgcgttcag tactgactgg
    16321 ttgtaacgtt caatcatcat gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac
    16381 cgtccaccgt caaacagcgg caatttcaga cccgggatga agttgaactg gcgactggtt
    16441 tttttgaata aggtatccag gtggatggca tcaaggccga aaaacgcttt gatatcaaag
    16501 ctcggataga acaacgcccg cgcggaatcc acctgatcta atgacgcctg aacataccag
    16561 cgcatggctt gcagatccgg gcgtctggcg agcaactcat aagagagtgt cgccggaatg
    16621 ccggtctgga ctcgtggtaa tgccacaggt ttgatctccg gcatatcgct ggctcccgcg
    16681 ccaatcaatg cgcgcagaga ttctctcgtt tcggtgattt gccctttgac ggcagcaatt
    16741 tgtttatcga ccgccagaat ctgtgcccgc gcgccgtgga aaggcacttg tgcttccaga
    16801 ccgtgcgcca ctttactttg gtgcgctttc accgcgtaat caatcacatc gcgagtttgt
    16861 tctaacagat cgagcatctg atagctggcc tgcatactgt aataaagctg cgctacgccc
    16921 gtggtcagcg atagctctac tgctgcggtt tctgccagcg cggcattatg cgcgccaatg
    16981 gcggcggcaa ccgctgagcg atgcacaccc cacaaatcaa gatccagtcc ggcaaacaaa
    17041 cctactgtgg cttccgtata gtacggcccg tccatcccca gtgcgggcgc atccatcgca
    17101 taagggctta aaaagccgtt cgccgagaca cgttggcggt taagcatccc taacgctgcc
    17161 acctgcaact gtgaaccggc atctaacaaa tcggcctgcg attgcgcttt ttcttcccgc
    17221 agtttcgctt cggcgagggt gtgtgaacca cttagcgtcc gttggatcag ggcatccaac
    17281 tgcgggtcat tgagttgttt ccaccactgc gcctgcggcc agccggagct ggcaagatga
    17341 atatcatcgg ccagtttgat ttgttccggt ttgagctgtt gatgaggcgc agaatcctta
    17401 cgtaccaggg cacagccgga aatcagcgtc gtgctgccga gaatgctgca caacagcaga
    17461 cgtgaaagtt gacgattgat cattgcgtgg ctccttgtgc ctgttccgtg gcgggcgtta
    17521 atgccggggc tgtccagtct ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt
    17581 ttaataaggt gggcaggatg gcctgcgagg tgggtgtctc ttcaagcgtt atttgaggag
    17641 aacggctgag tgcggttgcc agaccggcag cgtatttctc cagggcgtcg gcaaactgac
    17701 ctgctctgtc cggctgtagt gcgttatcca gcgcctgcgc cgaattccag gtggcatccc
    17761 aggcgtgaag gatatcgcgg ccctgacgaa taaccgtttg cgaacgttca atcagtaagg
    17821 cgcgttcttc gctgtccagt tgacgctcca gcgccacgcg ttggcacatc tcttcacagg
    17881 cattaaacgc cgcatgcaga ccgatacgaa tttgcagata agtgcgcaga gccgtgactt
    17941 cctgctggcg tgggatccgc attactttac ttaacatacc cagcgcgcca gccagttttt
    18001 gcggcagcgt gcgcgcttca ctttcaggcc agacaaaggt gtaaatcatc gcggacacca
    18061 cggtaccaat gatgataccc agggcgcgat cgcgaatttc caccaggtcg tacactgggc
    18121 caaaaacgtt ttcgagcgtg gcgagcgcga aggtgaccac catctgtgtg ccgatataag
    18181 aagagcgttc agagctggtg gcaatccatg cgcccaacag gaaaatcggt gccagcacaa
    18241 acagcaattc gacaatattg tccagccagg gcatgaccag tagcgtgaat aacagcgcca
    18301 gaatcgcgcc gcaaaaggcc ccgccaaaac gcagcaccat cttctggtac gacgaaccga
    18361 catttggatt agcgacgatc acgcaggtca gcatacaggt gtgaatgcct tcccagtcca
    18421 cgccgctgta aaaggtgtaa cagatcaaac aggcgagcaa tgtttttacc gcgtagcgta
    18481 tatagtctgg attggtaaaa gcatcggcga ccattgatgg cggtttggcg gcgggcgttg
    18541 gcggcgtatt cgggttcatc tggcccagtt gtaacaacgt ctggcagata ttctccaggt
    18601 tacattcccg tgccgccacc gcttcacttt cactgagccg ccagtcgctt tgccagcact
    18661 gaccttcagc aacggcatgc tgcagcttgt tgatttctga ggctaatttt tgccgaaatt
    18721 caataattgc ctgagaatca gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa
    18781 tgtaggttac cgttgccacg cagctttgcc accatgcgct ttgagttcgc cagttggcat
    18841 cgtccgcgag acaaaagaca ttcagttttt gcagcgccag cgcctctctt tcaatccgcg
    18901 tttcgggtag cggtgcgagg ctgtccgtca ggtggctaat ggcatcatca agccgatcat
    18961 taagcgcttg atgcatttgc gtaatggcac gattgggaaa ccacagcacg ccgattaacg
    19021 tcatcagcaa ggttggatag aggccaacaa cgatacacca cagcgttaag cgcacgacta
    19081 cttccggata gtcgagcatg gcggggaagg tttgcccgta aatggcgaca gcgaaaaaga
    19141 ccagtcccag ccgatgggtg cgcatcaaaa acatgcagcc catcaggatc ggcccggcga
    19201 tgatcaatcg gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga
    19261 tctccagcac cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg
    19321 actgaatacc gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaagtca
    19381 tcgagatcag aatcaccagc aggcagccta cccagagttg cacgatctgc ggcacgcggc
    19441 cgggtcgccg ctcgcttaac tcttcatgaa agaacgccag cagcctgacc accggtaatg
    19501 gcagggagtt gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat
    19561 gcggaacatt tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac
    19621 gcggacccag ttaatagaac gagacacttt tggcaggccg cccagcacca ggccgccgtc
    19681 atccggtagc acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc
    19741 actcatcagg cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg
    19801 gaagttggcg atcacgtacc agtgacgagt gtcgatgagg gtaaaaatag ggcgcatggc
    19861 agaagcaaat tgcccgacgg aggttttgag ggaaatgatc cggccatcaa atggcgcgcg
    19921 aacggtcgtc atttccagat gtagtttggt cagggcaata tccgcttcga ccgccgcacg
    19981 ttgcgccacc agcgcatcga cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa
    20041 tacggcatta agatccgctt ctgcggcgcg ctgcgccgtt cttgcacggt caacgtcttc
    20101 tgctgagaca aaaccctctt tcagtaatgg ctcggtgcgg cgtaatgtat ctgtggcctg
    20161 tttcgcggcg gcacgggctt tttctaccgt ggcattaacc gagtcggcac caaactgttg
    20221 ggcgtcaacg ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc
    20281 ggctttcgcc aggttggctt cgtacgggcg cgggtcgatg cggaacagca aatcgccctg
    20341 tttcaccgcc tggttgtcgg tgaccgccag ttctacaatg cgaccgctga cttccggcac
    20401 cacatcaatg gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg
    20461 ccagataacg aaaacaaggg caaccagcgc caacgcaacc actaacagag cagggaattt
    20521 actgcgaggt gcttttttcg gcgtactttc catgagcgcg tctcaaatag attagaaaaa
    20581 tgccagccag aacagcatgg cgtagagagc aaaaagggcg gtataaataa ttccgacaaa
    20641 tgccagattg atatttgccc g
//
LOCUS       DAZOZX010000067        20523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZOZX010000067 DAZOZX010000000
VERSION     DAZOZX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.067"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="ACHUPT_002384"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="ACHUPT_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0819932.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="ACHUPT_002385"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="ACHUPT_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR0819933.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="ACHUPT_002386"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="ACHUPT_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0819934.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHUPT_002387"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHUPT_002387"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0819935.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHUPT_002388"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHUPT_002388"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0819936.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="ACHUPT_002389"
     CDS             4451..4816
                     /locus_tag="ACHUPT_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0819937.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="ACHUPT_002390"
     CDS             4966..5484
                     /locus_tag="ACHUPT_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0819938.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHUPT_002391"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHUPT_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="ACHUPT_002392"
     CDS             6715..7197
                     /locus_tag="ACHUPT_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0819939.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHUPT_002393"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHUPT_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0819940.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHUPT_002394"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHUPT_002394"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0819941.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHUPT_002395"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHUPT_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0819942.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHUPT_002396"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHUPT_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0819943.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHUPT_002397"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHUPT_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0819944.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="ACHUPT_002398"
     CDS             10941..11732
                     /locus_tag="ACHUPT_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0819945.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="ACHUPT_002399"
     CDS             11753..13039
                     /locus_tag="ACHUPT_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0819946.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHUPT_002400"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHUPT_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0819947.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="ACHUPT_002401"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="ACHUPT_002401"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0819948.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="ACHUPT_002402"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="ACHUPT_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0819949.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="ACHUPT_002403"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="ACHUPT_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0819950.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="ACHUPT_002404"
     CDS             complement(16987..17277)
                     /locus_tag="ACHUPT_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0819951.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHUPT_002405"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHUPT_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0819952.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="ACHUPT_002406"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="ACHUPT_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0819953.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHUPT_002407"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHUPT_002407"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="ACHUPT_002408"
     CDS             19139..20380
                     /locus_tag="ACHUPT_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0819954.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5359 c         4815 g         5049 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DAZOZX010000068        20223 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZOZX010000068 DAZOZX010000000
VERSION     DAZOZX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.068"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            89..865
                     /gene="exuR"
                     /locus_tag="ACHUPT_002409"
     CDS             89..865
                     /gene="exuR"
                     /locus_tag="ACHUPT_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0819955.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1105..1188
                     /gene="yqiM"
                     /locus_tag="ACHUPT_002410"
     CDS             1105..1188
                     /gene="yqiM"
                     /locus_tag="ACHUPT_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR0819956.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1210..1872
                     /gene="yqjA"
                     /locus_tag="ACHUPT_002411"
     CDS             1210..1872
                     /gene="yqjA"
                     /locus_tag="ACHUPT_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0819957.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1876..2259
                     /gene="mzrA"
                     /locus_tag="ACHUPT_002412"
     CDS             1876..2259
                     /gene="mzrA"
                     /locus_tag="ACHUPT_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0819958.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2406..2760
                     /gene="yqjC"
                     /locus_tag="ACHUPT_002413"
                     /pseudo
     CDS             2406..2760
                     /gene="yqjC"
                     /locus_tag="ACHUPT_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2798..3103
                     /locus_tag="ACHUPT_002414"
     CDS             2798..3103
                     /locus_tag="ACHUPT_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0819959.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3106..3510
                     /locus_tag="ACHUPT_002415"
     CDS             3106..3510
                     /locus_tag="ACHUPT_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0819960.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3500..3799
                     /locus_tag="ACHUPT_002416"
     CDS             3500..3799
                     /locus_tag="ACHUPT_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0819961.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3985..4281
                     /locus_tag="ACHUPT_002417"
     CDS             3985..4281
                     /locus_tag="ACHUPT_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0819962.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4351..5337
                     /gene="yqjG"
                     /locus_tag="ACHUPT_002418"
     CDS             4351..5337
                     /gene="yqjG"
                     /locus_tag="ACHUPT_002418"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR0819963.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5630..5998
                     /locus_tag="ACHUPT_002419"
     CDS             5630..5998
                     /locus_tag="ACHUPT_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0819964.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="ACHUPT_002420"
     CDS             complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="ACHUPT_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR0819965.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7206..7907
                     /locus_tag="ACHUPT_002421"
     CDS             7206..7907
                     /locus_tag="ACHUPT_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0819966.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7930..8094
                     /gene="yhaL"
                     /locus_tag="ACHUPT_002422"
     CDS             7930..8094
                     /gene="yhaL"
                     /locus_tag="ACHUPT_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0819967.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8306..9616)
                     /gene="cyuA"
                     /locus_tag="ACHUPT_002423"
     CDS             complement(8306..9616)
                     /gene="cyuA"
                     /locus_tag="ACHUPT_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0819968.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9644..10975)
                     /locus_tag="ACHUPT_002424"
     CDS             complement(9644..10975)
                     /locus_tag="ACHUPT_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0819969.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="ACHUPT_002425"
     CDS             complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="ACHUPT_002425"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0819970.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12686..13075)
                     /locus_tag="ACHUPT_002426"
     CDS             complement(12686..13075)
                     /locus_tag="ACHUPT_002426"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0819971.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="ACHUPT_002427"
     CDS             complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="ACHUPT_002427"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0819972.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="ACHUPT_002428"
     CDS             complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="ACHUPT_002428"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0819973.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="ACHUPT_002429"
     CDS             complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="ACHUPT_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0819974.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="ACHUPT_002430"
     CDS             complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="ACHUPT_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0819975.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="ACHUPT_002431"
     CDS             complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="ACHUPT_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0819976.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5251 a         5287 c         4820 g         4865 t
ORIGIN      
        1 caatcatcaa atttatatat gaccaatggc atacccatga ataaagtggt atgataactt
       61 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      121 acaacttgct gctgacctga aagagcgcat tgaacagggc gtctatctgg tgggtgataa
      181 actgcctgcc gaacgcttta ttgccgatga aaagaacgtc agccgaacgg tagttcgtga
      241 agccatcatc atgctggaag ttgaaggcta tgtggaggtg cgtaaaggtt caggtattca
      301 tgtggtttcc aaccagccgc gtcatcagca ggcggctgac aataatatgg aattcgccaa
      361 ttacggtccg tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt
      421 cgcggcaact caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc
      481 gcgcggcgaa caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc
      541 cctggcgacg cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccagcgtag
      601 ccataacccg tactggaaaa aactacacga acacattgat tcccgtaccg ttgataactg
      661 gtgtgatgac cacgatcaaa ttctcaaggc gttaattcgc aaagatcctc atgctgctaa
      721 gctggcaatg tggcagcatc tggaaaacac caaaatcatg ttatttaacg aaaccagcga
      781 cgatttcgag ttcaatgccg accgctatct gttcgccgaa aacccggtag tgcatctcga
      841 tacggccacc agcggcagca aatgaaattc ctgtccgaca ggcgctgcaa tgcgcctgtt
      901 tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct
      961 cccctgcaat cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg
     1021 acctttgtta caattagatt caatttgaat ttatgctttt gaatgctttc ttatctcacg
     1081 ttttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac
     1141 agcaataaca ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag
     1201 gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa
     1261 accctggcca atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc
     1321 cttgaaaatg gcttgcttcc ggcggccttt ttaccgggcg acagtttact ggtattggtc
     1381 ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt
     1441 gccgccagcc tcggttgctg ggtcagctat attcaggggc gatggctggg caatacccgc
     1501 accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt
     1561 tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcatt tgtcagaaca
     1621 ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca gttcttcaac
     1681 tggatgagcg gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa
     1741 acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg
     1801 gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga
     1861 aatcgggggt aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc
     1921 actgttttac ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag
     1981 tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc
     2041 tggcatcacc ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact
     2101 ctgttaatta catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga
     2161 acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg
     2221 ctgacccggt tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata
     2281 tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact
     2341 accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt
     2401 caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt
     2461 atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg
     2521 aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg
     2581 ccaactgttc agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag
     2641 atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata
     2701 gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg cgactactaa
     2761 ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac acactacgga
     2821 acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc ttagctcatc
     2881 tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc aggcactgaa
     2941 acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa cccgtgtcgc
     3001 ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg gaattggcgc
     3061 tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc ggacactcat
     3121 cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt ttccatcatg
     3181 gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga ggaaaaagct
     3241 aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc atttggtctt
     3301 atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct gaatgcgatg
     3361 attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg gacgttacgt
     3421 aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa cgatcggcaa
     3481 ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta aggcgcaact
     3541 gcttagccag atccagcaac aacggctgga tatttccgcc agtagtcgtg aatggctgga
     3601 ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta agtctgcgct cctgggcgct
     3661 ggttggcagt agagtgatgg cgatctggac gattcgccat cctaatatgc tggtccgctg
     3721 ggccagacgc ggttttggcg tatggagcgc ctggcgtctg gttaaaacga ccctcaagca
     3781 gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta tccatcaaat
     3841 tttttgaata tttttgacag ttttccttgc taacaatcat cattcaccac gtttatgatt
     3901 ctctccatcg acagcaacga cgctaatagc gcgcccttgc acaaaaaaac aatcagcagc
     3961 ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt actggtagcg
     4021 cacattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac cgcttactct
     4081 ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgct gacggcattt
     4141 ttatttcaca gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca
     4201 atttctggcg gattcctgct gctggcaatt accggtccgg gcgcgtatag catcggccgc
     4261 ctgctgaata aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc
     4321 tcctcgtttt tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat
     4381 gacacctggt acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt
     4441 cgtaactggc tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca
     4501 gagaaagatc gttatcatct ctatgtttca ctcgcctgcc cgtgggcgca ccgcacgctg
     4561 atcatgcgca aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg
     4621 atgctggaaa acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc
     4681 tatcaacatg aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga
     4741 gttactgttc ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca
     4801 gaaatcatcc gcatgttcaa taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac
     4861 tacccaccag ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt
     4921 aacaacggcg tgtataaagc tggttttgcc accagccagc aagcctacga cgaggcggtg
     4981 gcgaaagtgt ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg
     5041 accggcaacc agctaaccga agccgatatt cgcctgtgga ctacgctggt gcgttttgat
     5101 ccagtgtatg tgacccactt caagtgtgat aagcaccgca tcagcgatta cctgaatctg
     5161 tatggcttcc tgcgcgatat ctaccagatg ccggggatcg ccgaaactgt caatttcgat
     5221 cacatccgta atcattactt ccgcagccat aagaccatca accctacggg gattatttca
     5281 attggtccgt ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa
     5341 aaggcacctt acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc
     5401 ggcgtgaacg ccttatcaaa cctacaaagc gtttatattc aatatgttac aatgaccgcg
     5461 taggccagat aagcgtagcg catctggcga ttttgcgctt gccatcagtc ttagcgcgcc
     5521 ttcacggcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     5581 cttaatttgc tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta
     5641 tctgaaagta ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     5701 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     5761 aggctggcaa cgcgctggtg gcgaaggcat cctgacgata atctacggta ttctggtgtt
     5821 tttaccgtgg tgggcggttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     5881 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     5941 ggcgggtacg ccaggcgaaa accgctttgg accagatcca aaactggaac aagaataatg
     6001 attaagcacc ttgtaaaagg tgcttcaaat tgttgacaaa gtgcgctttg tttatgccgg
     6061 atgcggcgta aacgccttat ccagcctaca aattgccaaa attcaatata ttgcagtgac
     6121 tgcgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcagc agtcagaagc
     6181 accttgtgaa aggtgcttaa tagattattt tccagcgaac aactttggaa tttcccgcag
     6241 acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc
     6301 gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat
     6361 cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat
     6421 cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc
     6481 acgagcggta tccgctaccg caattccacg atatttcaca cgcgtcactt cagataacgg
     6541 ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata
     6601 gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacgat
     6661 atccgcccgc ccctgctcca gccgttccca cgcccccgcc agcacttctg tgatgattgc
     6721 cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg
     6781 taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gagcttccgc
     6841 atcagtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct
     6901 cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc
     6961 ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc
     7021 tgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt
     7081 taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa
     7141 tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa
     7201 gaattatgat tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc
     7261 aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct
     7321 ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct
     7381 atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg
     7441 aaggcaatca tgttcaggcc agcgccggag aggcgttgct gctctctacc cagccgggtg
     7501 tcagctatag cgaacacaat ctcagcaaag acaaaccgtt aacgcgaatg cagctttggc
     7561 tggatacctg cccgcagcga gagaatccgc tgattcaaaa gctggcgctt aatatggaca
     7621 agcagcaatt aatcgcctcg ccagagggtg cgatgggaag cctgcaatta cgccaacaag
     7681 tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc
     7741 cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga
     7801 aagcggcgct aacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg
     7861 ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg
     7921 gagatgatga tgagtaaaaa atcgaccaaa aagcgccagc cagcgaagcc cgtggtggcg
     7981 aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa
     8041 gctatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag
     8101 gttgccggat gcggcgtaaa cgccttatcc ggcctacaaa acacaacaac atcaacaaat
     8161 taaacacccc gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac
     8221 acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc
     8281 cggcacaata ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct
     8341 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
     8401 caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta
     8461 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg
     8521 caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatag
     8581 tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg
     8641 ttgcggcaca aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat
     8701 gagaaagcat cagcgcacgc gccagtcgtt catcatccgc tccgaagtgt tctgctacaa
     8761 ccaccacagg cattattgca gtgatcccct ggttacccga gccggagtta ctcatagccg
     8821 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
     8881 aagagagatc tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat
     8941 gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat
     9001 cgagaataaa gcggatcgcc gcaaatggga cttcattgac gaacttcagg atctcagcca
     9061 gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc gccctctgcc acacacgcct
     9121 ggtgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
     9181 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
     9241 cattgcaagg ttcctggatc ttaacagaga ctttccccgc cgccagcagt gctttggcat
     9301 cggtaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac
     9361 ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga
     9421 cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac
     9481 cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc
     9541 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta
     9601 aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga
     9661 acagagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt
     9721 atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc
     9781 cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc
     9841 tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct
     9901 tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa
     9961 agcctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga
    10021 ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg
    10081 aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca
    10141 cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg
    10201 ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc
    10261 ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt
    10321 tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca
    10381 tcgacacgcc cagcgcagcg actaccagca gcttggtcag caccatgtcg gtcgaaattt
    10441 tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac
    10501 cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag
    10561 cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat
    10621 agagcgcacc taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt
    10681 aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca
    10741 ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct
    10801 gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc
    10861 cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac
    10921 gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt
    10981 ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg
    11041 gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa
    11101 aatcttcaca atcaagactt taaaataacg gagatggata agaattttct actaaattaa
    11161 tcacaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt
    11221 gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct
    11281 ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg
    11341 atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg
    11401 tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg
    11461 gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat
    11521 acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgctgccat tgaacaggcc
    11581 acgccaatct ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac
    11641 agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga
    11701 cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc
    11761 ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg
    11821 ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctaga aaccagctga
    11881 cgacgcagcg ctacggcacg acacggcaca ttgagcggac caggcagcac gccttcagtg
    11941 ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca
    12001 atttccgctt tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg
    12061 ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac
    12121 gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca
    12181 gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg
    12241 ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca
    12301 acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat
    12361 gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg
    12421 gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag atcgaccaca
    12481 atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg
    12541 gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg
    12601 aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc
    12661 ttgcgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca
    12721 gcttcacatc tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct
    12781 gatgctcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg
    12841 tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg
    12901 ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg
    12961 tctgcgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct
    13021 gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc
    13081 ctccggcgtc agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg
    13141 gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt
    13201 tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt
    13261 tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg
    13321 gttttacgta ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag
    13381 atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca
    13441 cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga
    13501 cgaccgtccg gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga
    13561 atcgactggg tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag
    13621 cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga
    13681 tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg
    13741 cgggcatatt tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat
    13801 gccatagtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac
    13861 ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc
    13921 atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt
    13981 gctgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag
    14041 agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc
    14101 attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga
    14161 tcgtcatttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg
    14221 aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc
    14281 atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc
    14341 agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca
    14401 aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt
    14461 tcctgtgcct gctgctcatt gaggacgcca gctttaaagt cgcgctcaat gtagatatcc
    14521 aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt cactgccgcc
    14581 agataagcga agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg gcgagagata
    14641 tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc
    14701 agctcctcac gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg
    14761 agatcggcaa attgcagttc gcgttcacgc accagataac tgatgccata cagcgctacg
    14821 cggcgatagt caccgataat gcgcccacga ccatagccat ctggtaaacc ggtcagcacg
    14881 ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg
    14941 gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg
    15001 tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc
    15061 gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga
    15121 gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg
    15181 atacggatgc cttccattac cttttcccac aattccgtgg tggcaggcgt cgcttcggcg
    15241 agaaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta
    15301 atttcgcttt tccagtccgt acctttaaag ccaagccatg cgtcagcgta cagcttatcg
    15361 ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca actaaattat
    15421 gcaaattctg cgggcgcgtt aactttacct aaatgaatgg catccaaagc aatcattttt
    15481 tcttcgttag tcggaataac gacgcaaatg acatgcgcat tttcactgga aacaattcgc
    15541 tcgccaaaag agttagaacg attattcatt tctgtatcaa tctctacgcc taatacagcc
    15601 aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat
    15661 ataattccat ccagccgacg taatgaagct gcatgtccgg caatatgacg ggcaattcgg
    15721 tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc
    15781 agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact
    15841 acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca
    15901 aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt
    15961 gaggtgtcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt gccaagatgc
    16021 gccacaacca ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa
    16081 acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat
    16141 ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca
    16201 aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa
    16261 tttgcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca
    16321 tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt
    16381 aaggccacgc tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca
    16441 ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca
    16501 ttttccgagt taataccgtc ggcaatacct gacattaata cttcacaatc gctggcatcg
    16561 agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca
    16621 ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat
    16681 ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag
    16741 agacggcgct ttacggatag catacatcgg caacaggcac agcagggatg cgataatcgg
    16801 tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt
    16861 ggagcccatg atgaagatca tgctgagagt attcagttta cccagcgaca ctttggtttt
    16921 gtcaccttta taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata
    16981 gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg catattccag
    17041 tgtaatcgcg aacgttgttt tcgtaccggt catggacgca aagtggttag ccagataaga
    17101 aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca gagtaaacag
    17161 gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat
    17221 ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt
    17281 agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat
    17341 ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc
    17401 aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag
    17461 gctggcgata aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa
    17521 ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg
    17581 tgcaaatccg agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata
    17641 aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt
    17701 tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag
    17761 acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat
    17821 cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc
    17881 gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga
    17941 ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat
    18001 taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc
    18061 ggaaataata ctgacggttt ttctattttg aatatattgg tctaatttac cgcttaataa
    18121 tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc
    18181 aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg
    18241 aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc
    18301 ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt
    18361 ttctgactgt acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat
    18421 accagcaatt aaaccaccac caccaattgg cacaatcacg ttatcgacat catagagatc
    18481 ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg
    18541 tgggataaaa atacgacctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt
    18601 gaagttatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga
    18661 ttttggcgca ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag
    18721 cgaaacccct tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc
    18781 atcggtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg
    18841 catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg
    18901 aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatatcatc
    18961 aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat
    19021 atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata
    19081 aaaacaaaca actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa
    19141 tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac
    19201 cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga
    19261 ttagaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga
    19321 acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca
    19381 tttctttgta acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc
    19441 cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg
    19501 gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt
    19561 tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac
    19621 gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg
    19681 ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc
    19741 accaccgctt cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta
    19801 atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt
    19861 ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg
    19921 gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact
    19981 tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac
    20041 gttaattacc tcattgacgg catgaagtgt atcaaaatga aataaacagg atatgtgcga
    20101 ccactcacaa attaactttc attactttcc aaggtatcgt tattacaatt aaacatcttt
    20161 aaatatcaac aagttaaagc ataaaaatca gcataaaccc tgatttttat caaaaaaaaa
    20221 atg
//
LOCUS       DAZOZX010000069        20035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZOZX010000069 DAZOZX010000000
VERSION     DAZOZX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.069"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACHUPT_002432"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACHUPT_002432"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0819977.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACHUPT_002433"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACHUPT_002433"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0819978.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3001)
                     /locus_tag="ACHUPT_002434"
                     /pseudo
     CDS             complement(1729..3001)
                     /locus_tag="ACHUPT_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3184..4389)
                     /locus_tag="ACHUPT_002435"
     CDS             complement(3184..4389)
                     /locus_tag="ACHUPT_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0819979.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4489..4845)
                     /locus_tag="ACHUPT_002436"
     CDS             complement(4489..4845)
                     /locus_tag="ACHUPT_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0819980.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4981..5088)
                     /gene="rprA"
                     /locus_tag="ACHUPT_002437"
     ncRNA           complement(4981..5088)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUPT_002437"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACHUPT_002438"
     CDS             complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACHUPT_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0819981.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6775..9831
                     /locus_tag="ACHUPT_002439"
     CDS             6775..9831
                     /locus_tag="ACHUPT_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0819982.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9828..10238
                     /gene="menI"
                     /locus_tag="ACHUPT_002440"
     CDS             9828..10238
                     /gene="menI"
                     /locus_tag="ACHUPT_002440"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0819983.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10338..10526
                     /locus_tag="ACHUPT_002441"
     CDS             10338..10526
                     /locus_tag="ACHUPT_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0819984.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11074..11442
                     /gene="sufA"
                     /locus_tag="ACHUPT_002442"
     CDS             11074..11442
                     /gene="sufA"
                     /locus_tag="ACHUPT_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0819985.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11451..12938
                     /gene="sufB"
                     /locus_tag="ACHUPT_002443"
     CDS             11451..12938
                     /gene="sufB"
                     /locus_tag="ACHUPT_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0819986.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12948..13694
                     /gene="sufC"
                     /locus_tag="ACHUPT_002444"
     CDS             12948..13694
                     /gene="sufC"
                     /locus_tag="ACHUPT_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0819987.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13669..14940
                     /gene="sufD"
                     /locus_tag="ACHUPT_002445"
     CDS             13669..14940
                     /gene="sufD"
                     /locus_tag="ACHUPT_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0819988.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14937..16157
                     /gene="sufS"
                     /locus_tag="ACHUPT_002446"
     CDS             14937..16157
                     /gene="sufS"
                     /locus_tag="ACHUPT_002446"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0819989.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16170..16586
                     /gene="sufE"
                     /locus_tag="ACHUPT_002447"
     CDS             16170..16586
                     /gene="sufE"
                     /locus_tag="ACHUPT_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0819990.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16735..17739
                     /gene="ldtE"
                     /locus_tag="ACHUPT_002448"
     CDS             16735..17739
                     /gene="ldtE"
                     /locus_tag="ACHUPT_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0819991.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACHUPT_002449"
     CDS             complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACHUPT_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0819992.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="ACHUPT_002450"
     CDS             complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="ACHUPT_002450"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0819993.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19769..19963)
                     /locus_tag="ACHUPT_002451"
     CDS             complement(19769..19963)
                     /locus_tag="ACHUPT_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0819994.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5239 a         4828 c         5126 g         4842 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg ttttgctgtt catttgcggt aattcattag
     2401 ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca
     2461 gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca
     2521 tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta
     2581 tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat
     2641 ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg
     2701 ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg
     2761 cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca
     2821 aaccgatacc ggaaattaaa taggcaatgc ccgcgttgtc agtggaaaac ttttctgaca
     2881 gagatgtcat attttgggca agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt
     2941 aaatacaaag aacagccagg ataaatggcg tgctgaaaga taatttcgtt accgtacgta
     3001 ttgttctccg acggtttaca taattactat actgaagatg attttccctg ggtacatggc
     3061 ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc
     3121 ttagttagcg cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt
     3181 gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct
     3241 gcgtaagccg ataaacagtg ccagtaaaaa accgatggca gtatcgaacc acataatatc
     3301 ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact
     3361 gcccgtactg taatagatcc ctgtagcttt acctttagcg taagggaaac gttcagccat
     3421 taacgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa
     3481 tattatcacc acataaaatg tgggatgcag gcagacggta aacagagcaa taaatgagat
     3541 aaaggtgtac agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt
     3601 aataaataca caaagcagcg aacccacggt gtagatactg agtagtttga tcgacatagt
     3661 gtatgacatg cctgcaacaa attgtccgta ctgtgccagc cactggctaa ccagataaaa
     3721 cgttgccatt gagatatagc catataaggt ataactggct aaatcgataa ttgaacagcg
     3781 atgttccgta gagctggtgg ttttctttat gacaggtaag cgacgacccg gatggggtgg
     3841 gaacgtacaa cggtataaaa acagagcgtt aataaacata atgcctgcag caatcataaa
     3901 ggaccaaccg aaccacagtt cagcccacac taacaggcta atgattagcg gtaataaaaa
     3961 ttgtccgctg gaaacaaatg ctttaattaa aatattggct gtcccaggtg agcgtggaaa
     4021 agcttccatc aaactgggat aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag
     4081 aaagccaaaa acataagcga taatgatgtt attggtctgc aggatgtcaa aaaagaaggc
     4141 catatagcag cacatcccga gcatgataaa agggcggcga ccaaagcgat cggataataa
     4201 tcctgcaaaa agcaagacac ttaatcgacc aatgcccagc gatgagataa ctatcgagac
     4261 acccgcggca ttagtctgcc aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat
     4321 gacgcccata ccatgcacca ggtaattaaa atacaaccca agtgcggtag ggtaataggg
     4381 atttttcata ggttctactt agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt
     4441 atccattcat tattgttgta acaacgacaa ttcctttctg tggaaaaatt aacaaagccg
     4501 ctccagatcg tgtgcatata attcagcggc taccgattga tagatcttcc agtcaataaa
     4561 attgccatca gggtagactt gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt
     4621 gttgccgatg cggttattta ttttctctat gatggcatga agatgttttt ttcgttgctg
     4681 acgcatagcc aacagttgct cgactacaca atcaggaatg gcgcatttgc cattttccca
     4741 ctgacgccag ctttcactgt caccggtctg ggcaatccag gtcgcgcatt catcaatagt
     4801 catggcaaaa atatggcgga gtgcctggag ttcataagcg ttcatcgtgt aatggctgca
     4861 tgaaataaac aataaaatat aaataacatt atcagcctgc tgacggcttg aagagagtca
     4921 cagtatcttg tgcaacatta ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg
     4981 ttaaaaaaaa gcccatcgtg ggagatggga aaagactaca cacagcaatt cgttgtttca
     5041 ctcaggggat ttccatgctt ataaatcaat atgttgattt ataaccgtga gctaatagta
     5101 ggcatacgga ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat
     5161 ttgcgacagg tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca
     5221 aatatcaatt agcggtgatt aacaatcagg cggcagagcc gcctgatgag aaattactta
     5281 ttcggtttct cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggtggc
     5341 gggacttctt ccacccacgc ggcaaacaga cgccaggaaa cggctaacag aaccggacca
     5401 ataaacagac cgatcatccc gaaagcaatc aaaccaccaa taacgccaga gaggatcagg
     5461 atcagcggta aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg
     5521 ccaaccacac cgctccacac taacaatacc gttccccagg tggtatcgcc ggtccagtag
     5581 agccagataa tcgccggaat cagtaccggc aacgggccaa gctggacaag gcaggagagg
     5641 atcattaaca ccgttagcaa cgttgcataa ggtacgccgg atacggcgag gccgataccg
     5701 ccaagcactg cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc
     5761 tgtgccgcca gcagcacggc ggcatctccg cgaacgcctg ccagacgggt tgcaaaatgg
     5821 cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc
     5881 agcgcacaat gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg
     5941 gtggtgccaa tataagggcg gactttcgcc atgatcgccg taccccccat atccagcaag
     6001 ttgtgccagc ctgcatacag cttcgcgcca atcaccggaa tggtattaag ccacgccaga
     6061 tcgggtaacg tcatgtcgcc gctggaaatg gctttaatta gcgggccgct gccgtcgacg
     6121 atactgttaa ccagcaaagc aatagggatg ataaacacca tcactaataa cagcgtcatc
     6181 accagaacgg cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc
     6241 caggtggcga taaccaccgt accggcccat gcaaagccga gaataaaggg ttgaacaatc
     6301 cacagacatg ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca
     6361 tccctgggct gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt
     6421 cgacgcgacg tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt
     6481 aacaggccga ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa
     6541 ctcaggcaac acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc tgcggctacg
     6601 ttcgttcacc ccagtcactt actattgtaa gctcctggag ttcactcgct tgccgccttc
     6661 ctgcaactcg aaatccatag agtaaataaa catcaccttt cagtgcgcta ttgcagccgc
     6721 ctgaaagaat agtgtatcga cctacatcac agaccgcagg aaagggtcaa tataatgatt
     6781 ccacagattt ccctggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg
     6841 gagcaacaag gttttaccgg cgatgcggcg acaagttatg ccgatcgtct gacaatgtcg
     6901 accgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca
     6961 gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct gatctttacc
     7021 ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat
     7081 atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt
     7141 gaggccgggg tgataaaaga ccaactcaat cagtacctga aaccgttcgg ctactttttt
     7201 gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca
     7261 tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg
     7321 gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgagct ggcggaaacg
     7381 ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt
     7441 cagcaacgcc agttaatcat cgacaacttc cccaaactta accgctttct taccggttac
     7501 gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg
     7561 ggttcagaag ggacgctggc ctttattacc gaagcgcggc tggatattac gcgcttgcct
     7621 aaagtgcgcc gtctggtgaa tgtcaaatat gactcttttg actctgcgct gcgtaacgcg
     7681 ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat
     7741 ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga tatgcctgac
     7801 aaagagatgc tcggactgaa cattgtggaa tttgctggtg atgatgaggc gctgattgac
     7861 gagcgagtta atgcactctg tgtgcggctt gatgagctga ttgccagcca acaggcaggt
     7921 gtgattggct ggcaggtgtg ccgcgagctg gcgggggttg aacgtatcta tgcgatgcgc
     7981 aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct
     8041 gaggatacct gcgtaccgcc ggaacatctg gccgattata ttgctgaatt tcgcgcgctg
     8101 ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac
     8161 gtccgtccgg cactggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct
     8221 gatgacgtgg tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa
     8281 ggttttcgcg ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc
     8341 aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca
     8401 gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg
     8461 cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt
     8521 ttatgcttca actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac
     8581 cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg cttgttggcg
     8641 gatcgcggcg ttgacccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg
     8701 cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc
     8761 tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcttg ttcgacccag
     8821 tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc
     8881 cgttatttac gcccgcttcg cgaccacctc gttgctacgg tcgagagcta cgcaccgctg
     8941 atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc
     9001 tcggaaaaac atatcggcat ggttgatttg ccactgctgt cggtcccctc gctacaacaa
     9061 caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca
     9121 gagcagaaag cgcgcacggt gttggtggtg caggacccct ttaccagcta ttacgatgcg
     9181 caagtggtgg cggattttgt ccgtctggtt gaaaaattag gtttccagcc tgtgttactg
     9241 ccattttcgc caaatggcaa agcccagcat atcaaaggct ttcttaatcg ttttgcgaag
     9301 acggcgaaaa agacggcgga tttcctcaac cgtatggcta agcttgggat gccaatggtg
     9361 ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc gctgggcgag
     9421 gaacgtggcg agtttaacgt cttacttgcg aatgaatggc tggcaagcgc acttgagtca
     9481 catccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaggtt
     9541 accgctttgc cgggcgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa
     9601 ctggaaaatg tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa
     9661 aaccatgaaa attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg
     9721 ccgcgtaacc gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa
     9781 ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac
     9841 ggaaaatcac cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg
     9901 atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc
     9961 ggacaaagca gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta
    10021 tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa
    10081 tcaatgctaa tcacgtccgc tcggcacgag aagggcgggt gcgcggcata tgtaaaccgt
    10141 tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt
    10201 tgtgctgttc gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga
    10261 cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt
    10321 ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt
    10381 tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag
    10441 aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact
    10501 tcgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac
    10561 ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat
    10621 attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga
    10681 ttattcttat cgccccttca agagctaagc cactgagagt gccggagata agcgccggat
    10741 ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc
    10801 tgcacttttt tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca
    10861 cagccttaat gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc
    10921 gtgcctgtta acccacacat caggggctat gcttattaaa agcaggcaaa aagaatgttt
    10981 tacggtaaag cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt
    11041 tatacgctga aagcgatgaa gtgaggtaaa tagatggaca tgcattcagg aacctttaac
    11101 ccacaggatt tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgc
    11161 gagctggtgg caaagcagcc aggtatggtc ggcgtgcgct taggtgtgaa gcaaacgggc
    11221 tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg
    11281 tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaat cgatgccgtt tattgatggc
    11341 acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa
    11401 gcccagaatg aatgtggctg tggcgaaagc tttagggtat aggcggtact atgtctcgta
    11461 atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaac tataaagaag
    11521 gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc
    11581 gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct
    11641 atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga
    11701 attatcagga ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg
    11761 cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg
    11821 aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg
    11881 ccattttcga ctctgtttcg gttgccacca cttatcgcga aaaactggcg gagcagggaa
    11941 ttattttctg ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc
    12001 tcggcaccgt ggtgccgggg aatgacaact tctttgcagc acttaatgcg gcggtagcct
    12061 ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct
    12121 attttcgcat taacgcggaa aaaaccgggc agtttgagcg cacaattctg gtggccgacg
    12181 aagacagcta cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt
    12241 tgcacgcggc agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg
    12301 tacaaaactg gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc
    12361 gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag
    12421 cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt
    12481 actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca
    12541 tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga
    12601 acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc
    12661 agtgcgactc aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat
    12721 gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac atcacgtatt ggtgaagatc
    12781 aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta
    12841 acggtttctg caaagacgtg ttctcagaac tgccgctgga atttgccgtt gaagcacaaa
    12901 aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta
    12961 aagatttaca cgtcagcgtg gaagataaag ctatcctgcg tggattaagc ctcgacgttc
    13021 gtcccggcga agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg
    13081 caacgcttgc cgggcgcgaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca
    13141 aagatttgct tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt atggccttcc
    13201 agtatccggt ggagattcca ggcgtcagta accagttttt cctgcaaacg gcacttaatg
    13261 cagtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg
    13321 aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg
    13381 gtttttccgg cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg
    13441 agttatgcat tcttgatgag tcggactccg ggctggatat tgacgcatta aaagtggtcg
    13501 ccgatggcgt gaactcgctg cgtgatggca agcgctcatt catcattgtt acgcactacc
    13561 aacgcattct cgactacatc aagcctgatt acgttcatgt gctatatcag ggacgaattg
    13621 tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta
    13681 ccgaacagca gtaatgcgct gcaacagtgg catcacttgt ttgaagctga agggacaaaa
    13741 cgctcaccgc aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt
    13801 aaacatgaaa actggaaata tacgccgctg gaagggctga ccaatagtca gtttgtcagc
    13861 attgcgggag atatatcccc acagcagcgt gatgccttag cgttaacgtt agacgccgtg
    13921 cggctggtgt ttgtcgatgg acgttacgtg cccgcgctga gcgatgcaat tgaaggcagc
    13981 ggatatgaag tgagcattaa cgacgaccgt caggggttac ccgacgctat tcaggcggaa
    14041 gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc
    14101 ggtcaacggc cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa
    14161 gaggtgaaca ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg
    14221 gtgatcgaac attttgtcag ccttaatgat gctcgtcatt ttaccggggc acggttcact
    14281 atcaatgtcg cagcgaacgc ccacttgcag catatcaagc tggcgtttga aaacccggtc
    14341 agtcaccact ttgcccataa cgatttgttg ctggctgacg atgccaccgc atttagccat
    14401 agtttcctgc tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa
    14461 aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc
    14521 cgtacctggc tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc
    14581 gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc
    14641 aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat
    14701 acaaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg
    14761 gggcgtattg atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat
    14821 gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag
    14881 gggcttaaac agcaggtgct ggcccgaatc ggtcaacggc tgccaggagg tgcaagatga
    14941 ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt
    15001 tgccgctggc ttatctcgac agcgccgcca gtgcacagaa accgagccag gtgattgacg
    15061 ccgaggccga gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa
    15121 gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg
    15181 cccgttcggc ggaagagctg gtgtttgtcc gcggcacgac ggaagggatc aatctggtcg
    15241 ccaatagctg gggcaacagc aatgtgcggg cgggcgataa catcatcatc agtcagatgg
    15301 agcaccacgc taacattgtc ccctggcaga tgctttgcgc acgcgttggc gcagagctgc
    15361 gtgtgatccc tctcaacccc gacggtacgc tgcaactgga gacgctgcct acgctgtttg
    15421 atgagaaaac tcgcctgctg gcaattactc atgtctccaa cgtgcttggc acagaaaatc
    15481 cactggcgga aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg
    15541 ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct ggattgcgac ttttacgtgt
    15601 tctccgggca taaactgtat ggccccaccg gaattggcat tctttatgtg aaagaagcct
    15661 tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga
    15721 gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg
    15781 ggggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaatagca
    15841 tagccgagta tgaacagaat ctgatgcatt atgcgctatc acagctggaa tctgtaccgg
    15901 atctcactct ctatggccaa caaaacaggc ttggcgttat tgcttttaat ctcggtaaac
    15961 accacgccta tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac
    16021 atcactgcgc tatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc
    16081 tggccatgta taacacccat gaagaagtgg agcgtctggt gaccggcctg caacgtattc
    16141 accgtctgct gggataacag ggaggcacta tggctttatt gccggataaa gaaaaattgc
    16201 tgcgtaattt tttacgctgc gccaactggg aagagaaata tctctacatt attgagctgg
    16261 gccagcgtct gccagaatta cgcgccgaag acggaagccc acaaaatagc attcagggct
    16321 gccagagtca ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt gaattgcagg
    16381 gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc
    16441 agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc
    16501 tcacccaaca tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc
    16561 gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca
    16621 ttgccggatg gtcaatgttt acactgaccg gcacaggagt gttttggttt caggtgaaca
    16681 taagcaccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa
    16741 cgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg
    16801 gcggttgatt atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat
    16861 acggtgcaag aaggggataa aaaccttcag gctattgccc gacgttttga tactgcagca
    16921 atgttgatcc ttgaagccaa taacactatc gccccggtgc caaaacctgg tacgacgata
    16981 actattcctt cacaactgtt attacctgat gcaccgcgtc aggggattat cgttaacctt
    17041 gcagagctgc gcctttatta ttatccgccg ggagaaaata ttgtgcaggt ttatccaata
    17101 ggtattggat tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg gcagaaaatc
    17161 cctaacccaa cctggacgcc tacggcaggc attcgtcagc gttcgctgga gcgtggcatt
    17221 aaattaccgc cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc
    17281 ctcgcgcatg gtaatggcga atacctcatt catggcacca gtgcgccgga cagcgttggt
    17341 ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc
    17401 agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggaa
    17461 cctaacggga tgcgttatgt tgaagtacat cgaccactat cagcagaaga acagcaaaac
    17521 gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct
    17581 gtagatcaga agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg
    17641 agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt
    17701 gaaccagagc aagggaatat gttacgcgcg acgcagtagt ggtaaatagc agacaaaaaa
    17761 atggcgcaca atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta
    17821 gccatgttgt ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg
    17881 gaacgcattg cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg
    17941 tcagaagaca gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc
    18001 aggattaccg cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag
    18061 ttaatctcca tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt
    18121 tgatgggaag gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta
    18181 gataaaaaaa ttgagagaaa acaggatgct tccatcggat tcatcttaaa taaagtgaga
    18241 ttatatagtg aaatccgatt tgatttttta ttgcttctgg ttatcgatta aataaaaaaa
    18301 gcgcccatca gggcgcttcg atatacaaat taattcacaa aagcaatatt acaggacgtg
    18361 aacagatgcg gtgttagtag tgccgctcgg taccagtgca ccagaaacca taactacgac
    18421 gtcacctttg tgtgccagac cgctctgcag agccagttct ttacccagac ggtagaaatc
    18481 atcagtagaa gtgatctctt taaccagctg cggcacaacg cctttgctca gtaccaactg
    18541 atgagccgtt ttttcgttgg tggtcagtgc caggatggtg gcatccggga agtatttacg
    18601 tactgcgcga gcagatttac cgccctgggt agcaaccacg atcagcggag catccagttt
    18661 ttcagcagtt tcaacggcac cacggcatac cgcttcggtg atgcgcagtt tacggttgtc
    18721 attgttgaac tcgagacggc tgttcatcac gcggtcggta cgttcgcaga tggtcgccat
    18781 gatagaaacc gcttccagcg ggtatttacc ttttgcggat tcaccagaca gcatcactgc
    18841 gtcagtaccg tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg
    18901 gtttttgatc atggaatcca gcatctgggt cgcagtgata acgactttac gtgcacggat
    18961 acatttttcg atcatcatct tctgggcgaa gataacttct tctaccggga tttctacacc
    19021 caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt
    19081 gaggccttcc tggttttcga ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt
    19141 caggtgctca cggatttcga taacgtcaga acgcttacga ataaaggaag cagcaacaaa
    19201 gtctacgcct ttttcgcaac caaagatcag gtcctgtttg tctttttcag ccagtgctgg
    19261 cagagcaatg gaaacgccag gcaggttcac acctttgttt tcgcccaggt caccgttgtt
    19321 cagcacttta cagataactt tgttaccttc aatggcggta acttccatac cgatcagacc
    19381 atcgtcaacc agtacggtgt tgccaacaga caggtcagta gtgaaacctt catacgttac
    19441 cgcaaccatt tcgctgttgc cgataacaga tttatcagtg gtgaaagtaa aggtctgacc
    19501 agctttcaga gaaacatcgt taccgccttc cagtttcatg gtgcggattt ccggaccttt
    19561 ggtatcaagc aggatagcgg cggttttacc agttttgctc atcacgttgc gcagattctg
    19621 aatgcgctga ccgtgttctg catagtcacc atgagagaag ttcagacgca taacgttcat
    19681 gccagcgtcc agcattttag ctaacatctc ttcagattcg gttttcggtc cgatggtgca
    19741 aacaattttg gtctttttca tgacagtctt agtctttaag ttgagaagga tgggagaaac
    19801 ttgctttctg ggcgcactgc cggaaagcga atccggtatt acgaaagtgg tggtgcctcg
    19861 ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc
    19921 aacgtgagtg tctgtttttg agtcgttgag ttctacagtc caaagtgctg aaaccattca
    19981 agagtcaatt ggcgcgcatt atacgctgaa attaatccaa aaggaaaaca aaaac
//
LOCUS       DAZOZX010000070        20031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZOZX010000070 DAZOZX010000000
VERSION     DAZOZX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.070"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            251..1345
                     /gene="gcvT"
                     /locus_tag="ACHUPT_002452"
     CDS             251..1345
                     /gene="gcvT"
                     /locus_tag="ACHUPT_002452"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0819995.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1369..1758
                     /gene="gcvH"
                     /locus_tag="ACHUPT_002453"
     CDS             1369..1758
                     /gene="gcvH"
                     /locus_tag="ACHUPT_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0819996.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1877..4750
                     /gene="gcvP"
                     /locus_tag="ACHUPT_002454"
     CDS             1877..4750
                     /gene="gcvP"
                     /locus_tag="ACHUPT_002454"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0819997.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5017..5760
                     /locus_tag="ACHUPT_002455"
     CDS             5017..5760
                     /locus_tag="ACHUPT_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0819998.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5817..7250)
                     /gene="bglA"
                     /locus_tag="ACHUPT_002456"
     CDS             complement(5817..7250)
                     /gene="bglA"
                     /locus_tag="ACHUPT_002456"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0819999.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            7295..7605
                     /gene="yqfB"
                     /locus_tag="ACHUPT_002457"
                     /pseudo
     CDS             7295..7605
                     /gene="yqfB"
                     /locus_tag="ACHUPT_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            7769..8428
                     /locus_tag="ACHUPT_002458"
     CDS             7769..8428
                     /locus_tag="ACHUPT_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0820000.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8624..9604)
                     /gene="ygfZ"
                     /locus_tag="ACHUPT_002459"
     CDS             complement(8624..9604)
                     /gene="ygfZ"
                     /locus_tag="ACHUPT_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0820001.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9847..10113
                     /gene="sdhE"
                     /locus_tag="ACHUPT_002460"
     CDS             9847..10113
                     /gene="sdhE"
                     /locus_tag="ACHUPT_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0820002.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10094..10501
                     /locus_tag="ACHUPT_002461"
     CDS             10094..10501
                     /locus_tag="ACHUPT_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0820003.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10541..11062)
                     /gene="fldB"
                     /locus_tag="ACHUPT_002462"
     CDS             complement(10541..11062)
                     /gene="fldB"
                     /locus_tag="ACHUPT_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0820004.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11174..12070
                     /gene="xerD"
                     /locus_tag="ACHUPT_002463"
     CDS             11174..12070
                     /gene="xerD"
                     /locus_tag="ACHUPT_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0820005.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12095..12805
                     /gene="dsbC"
                     /locus_tag="ACHUPT_002464"
     CDS             12095..12805
                     /gene="dsbC"
                     /locus_tag="ACHUPT_002464"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0820006.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            12811..14544
                     /gene="recJ"
                     /locus_tag="ACHUPT_002465"
     CDS             12811..14544
                     /gene="recJ"
                     /locus_tag="ACHUPT_002465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0820007.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14635..15733
                     /gene="prfB"
                     /locus_tag="ACHUPT_002466"
     CDS             join(14635..14709,14711..15733)
                     /gene="prfB"
                     /locus_tag="ACHUPT_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0820008.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15743..17260
                     /gene="lysS"
                     /locus_tag="ACHUPT_002467"
     CDS             15743..17260
                     /gene="lysS"
                     /locus_tag="ACHUPT_002467"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0820009.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17303..17851)
                     /gene="idi"
                     /locus_tag="ACHUPT_002468"
     CDS             complement(17303..17851)
                     /gene="idi"
                     /locus_tag="ACHUPT_002468"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0820010.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17906..17977
                     /locus_tag="ACHUPT_002469"
     CDS             17906..17977
                     /locus_tag="ACHUPT_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820011.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(17974..18099)
                     /gene="yqfG"
                     /locus_tag="ACHUPT_002470"
     CDS             complement(17974..18099)
                     /gene="yqfG"
                     /locus_tag="ACHUPT_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0820012.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18101..19549)
                     /gene="uacT"
                     /locus_tag="ACHUPT_002471"
     CDS             complement(18101..19549)
                     /gene="uacT"
                     /locus_tag="ACHUPT_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR0820013.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4939 a         5004 c         5374 g         4714 t
ORIGIN      
        1 tcatcacatc atctttgtat agaaattggt gtattttttg gttttttatt ctgtcgcgat
       61 ttttgcattt tttaaccata agctaatgtg atgatcaatt ttaccttatg gttaacagtc
      121 tgtttcggtg gtaagttcag gcaaaagaga acgattgcgt tggggaccgg gagtggctcc
      181 gatgctgggt tttgtggtga taatttcacc atgaaaaagt tgtcagcccc gcttattcaa
      241 tgaggacaag atggcacaac agactccttt gtacgaacaa cacacgcttt gcggcgctcg
      301 catggtggat ttccacggct ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca
      361 tcatgcggta cgtaccgatg ccggaatgtt tgatgtgtcg catatgacca tcgtcgatct
      421 ccgcggcagc cgcacccggg agtttctgcg ttatctgctg gcgaacgatg tggcgaagct
      481 caccaaaagc ggcaaagccc tttactcggg gatgttgaat gcctctggcg gtgtgataga
      541 tgatctcatc gtctactact ttactgaaga tttcttccgc ctcgttgtta actccgccac
      601 ccgcgaaaaa gacctctcct ggattaccca acacgctgaa cctttcggca tcgaaattac
      661 cgttcgtgat gacctttcca tgattgccgt gcaagggccg aatgctcagg caaaagctgc
      721 cacactgttt aatgatgctc agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt
      781 gcaggtgggc gatctgttta ttgccaccac tggttatacc ggtgaagcgg gctatgaaat
      841 tgcgctgccc aatgaaaaag cggccgattt ctggcgtgcg ctggtggaag cgggcgttaa
      901 gccctgcggt ctgggggcgc gtgacacgct gcgtctggaa gcgggcatga atctttatgg
      961 tcaggagatg gacgaaacta tttctccttt agccgccaac atgggctgga ctatcgcctg
     1021 ggaaccgaca gatcgtgact ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg
     1081 tacagaaaaa ctggttggtc tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc
     1141 ggtacgcttt accgatgcgc agggcaacca gcatgaaggc attatcacca gcggtacttt
     1201 ctccccgacg ctgggttaca gcattgcgct ggcgcgcgtg ccggaaggta ttggcgaaac
     1261 ggcgattgtg caaattcgca accgtgaaat gccggttaaa gtgacaaaac ctgtttttgt
     1321 gcgtaacggc aaagccgtcg cgtgatttac ttttttggag attgattgat gagcaacgta
     1381 ccagcagaac tgaaatacag caaagaacac gaatggctgc gtaaagaagc cgacggcact
     1441 tacaccgttg gtattaccga acatgctcag gagctgttag gcgatatggt gtttgttgac
     1501 ctgccggaag tgggcgcaac ggttagcgcg ggcgatgact gcgcggttgc cgaatcggta
     1561 aaagcggcgt cagacattta tgcgccagta agcggtgaaa tcgtggcggt aaacgacgca
     1621 ctgagcgatt ccccggaact ggtgaacagc gaaccgtatg caggcggctg gatctttaaa
     1681 atcaaagcca gcgatgaaag cgaactggaa tcactgctgg atgcgaccgc atacgaagca
     1741 ttgttagaag atgagtaacg gctttattcc tcttctgcgg gagaggatca gggtgaggaa
     1801 aatttatgcc tcaccctcac tctcttcgta aggagagagg ttcacaattc actgcacgtt
     1861 tcaggaacca tcgctcatga cacagacgtt aagccagctt gaaaacagcg gcgcttttat
     1921 tgaacgccat atcggaccgg acgccgcgca acagcaagaa atgctgaatg ccgttggtgc
     1981 acaatcgtta aacgcgctga ccggccagat tgtgccgaaa gatattcagc ttgcgacacc
     2041 accgcaggtt ggcgcaccgg cgaccgaata cgccgcactg gcagaactca aggctattgc
     2101 cagtcgcaat aaacgcttca cgtcttacat cggcatgggt tacaccgccg tgcagctacc
     2161 gccggttatc ctgcgtaaca tgctggaaaa tccgggctgg tataccgcgt acactccgta
     2221 tcagcctgaa gtctcccagg gccgccttga agcactgctc aacttccagc aggtaacgct
     2281 ggatttgact ggactggata tggcctctgc ttctcttctg gacgaggcca ccgctgccgc
     2341 cgaagcaatg gcgatggcga aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt
     2401 ggcttccaat gtgcatccgc aaacgctgga tgtggtccgt actcgtgctg aaacctttgg
     2461 ttttgaagtg attgtcgatg acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt
     2521 gctgttacag caggtaggca ctaccggtga aattcacgac tacactgcgc ttattagcga
     2581 actgaaatca cgcaaaattg tggtcagcgt tgccgccgat attatggcgc tggtgctgtt
     2641 aactgcgccg ggtaaacagg gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt
     2701 gccgatgggc tacggtggcc cacacgcggc attctttgcg gcgaaagatg aatacaaacg
     2761 ctcaatgccg ggccgtatta tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg
     2821 catggcgatg cagactcgcg agcaacatat tcgtcgtgag aaagcgaact ccaacatttg
     2881 tacttcccag gtactgctgg caaacatcgc cagcctgtat gccgtttatc acggcccggt
     2941 tggcctgaaa cgtatcgcta accgcattca ccgtctgacc gatattctgg cggcgggcct
     3001 gcaacaaaaa ggtctgaaac tgcgccatgc gcactatttc gacaccttgt gtgtggaagt
     3061 ggccgacaaa gcgggcgtac tgacgcgtgc cgaagcggct gaaatcaacc tgcgtagcga
     3121 tatcctgaac gccgtgggga tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca
     3181 gcttttcagt gtgctgctgg gcgataacca cgggctggag atcgacacgc tggacaaaga
     3241 cgtggctcac gacagccgct ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac
     3301 ccatccggtg tttaatcgct atcacagcga aaccgaaatg atgcgctata tgcactcgct
     3361 ggagcgtaaa gatctggcgc tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa
     3421 actgaacgcc gccgccgaga tgatcccaat cacttggacg gaatttgccg aactgcaccc
     3481 gttttgcccg ccggaacagg ctgaaggtta tcagcagatg attgcgcagc tggctgactg
     3541 gctggtgaaa ctgaccggtt acgacgccgt ttgtatgcag ccgaactccg gcgcacaggg
     3601 cgaatacgcg ggcctgctgg cgattcgtca ttatcatgaa agccgcaacg aagggcatcg
     3661 cgatatctgc ctgatcccgg cttctgcgca cggaactaac cccgcttctg cacatatggc
     3721 aggaatgcag gtggtggttg tggcgtgtga taaaaacggc aacatcgatc tgactgatct
     3781 gcgcgcgaaa gcggaacagg cgggcgataa cctctcctgt atcatggtga cttatccttc
     3841 tacccacggc gtgtatgaag aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg
     3901 cggtcaggtt taccttgatg gcgcgaacat gaacgcccag gttggcatca cctcgccggg
     3961 ctttattggc gcggacgttt cgcacctcaa cctgcataaa actttctgca ttccgcacgg
     4021 cggtggtggt ccgggtatgg gaccgatcgg tgtgaaagcg catctggcac cgtttgtacc
     4081 gggtcatagc gtggtgcaaa tcgaaggcat gttaacccgt cagggcgcag tttctgcggc
     4141 accgttcggt agcgcctcta tcctgccaat cagctggatg tacatccgca tgatgggcgc
     4201 agaagggctg aaaaaagcaa gccaggtggc aatcctcaac gccaactata ttgccagccg
     4261 cctgcaggat gccttcccgg tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg
     4321 tattctcgat attcgcccgc tgaaagaaga aaccggcatc agcgagctgg acattgccaa
     4381 gcgcctgatc gactacggtt tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct
     4441 gatggttgaa ccgactgaat ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat
     4501 gctggctatc cgcgcagaaa ttgaccaggt gaaagccggt gtctggccgc tggaagataa
     4561 cccgctggtg aacgcgccgc acattcagag cgaactggtc gccgagtggg cgcatccgta
     4621 cagccgtgaa gttgcggtat tcccggcagg cgtggcagac aaatactggc cgacggtgaa
     4681 acgtctggat gatgtttacg gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga
     4741 ataccagtaa ttcactgatt cgagtatttt ctaaaggcgc ttcggcgcct ttttagtcag
     4801 atgacaaagt acaaaagtgc tcagacagtc ccctcgcccc tttggggaga gggttagggt
     4861 gaggggaaca ggccggcact ggcgcgaata tttaccctca ccccgaccct ctccctgaaa
     4921 gggcgagggg gaaaagcgtg ccaacattga agattgagcc agtttgttag caatctcaaa
     4981 gatacgtcaa cgaattaatt tttctcggaa aaacaaatgg ctatagcact tgtgaccggt
     5041 ggcagtcgcg gcatcgggcg ggcaactgca ttactgttgg cgcaagaagg gtatacggtg
     5101 gcggttaatt atcagcaaaa cctccacgcg gcgcaggaag tgatgaactt aataatgcaa
     5161 gccggtggca aagcattcgt gctccaggcg gatatcagcg acgaaaacca ggtcgttgcg
     5221 atgtttacag caatcgatca gcacgatgaa ccgctagcag cgctggtcaa taacgccggg
     5281 atcttgttta cccagtgcac cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt
     5341 tccaccaacg tgacgggata ttttctctgc tgccgcgagg cggtaaaacg catggcgctt
     5401 aaaaatggtg gcagtggcgg cgctatcgtc aatgtctctt cggtggcctc acggttgggt
     5461 tcgccagggg aatatgttga ttacgcggca tcgaaagggg cgattgattc gttaaccacc
     5521 ggactatcgc tggaagtcgc cgcgcagggg atccgcgtta actgcgtgcg gccagggttt
     5581 atttataccg aaatgcacgc cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg
     5641 aacatcccca tgcagcgtgg tggacaggca gaagaggtcg cgcaggccat tgtctggcta
     5701 ctaagtgata aagcctctta cgtcacggga agttttatcg atttggcggg cgggaaataa
     5761 aacagggaag ttgtctgacc ggatgcaaca agtattgcat ccggtacttc atcgacttaa
     5821 agcttctcgc cgttgctggc aatcacctct ttgtaccagt taaagctctt cttacgtgaa
     5881 cgcgacatat cgccagtacc gtcgtcatgt ttattcacat agataaagcc gtagcgtttg
     5941 ctgtactgcc cggtagtgaa tgacacgcag tcgatgcagc cccacggtgt gtagcccatc
     6001 agatccacac catcgtaagt cactgctttt ttcatctctt caatatgggc gcgcaggtag
     6061 tcaatgcggt agtcgtcgtt gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa
     6121 ccgttttcga caataaacag cggcctctga taacgctcat acagttcgca aagggcatag
     6181 cgcagaccta ctggatcaat ctgccagccc cagtcagacg ctttaacata cgggttcggt
     6241 acgctgcctt caaaaccaga gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg
     6301 gtcatgtaat agctgaaacc aagataatcg caggtgcctt cgcgcagtac atcgagatcg
     6361 ccgtcttcca ttttgatatt aaatccgcga cgttcccact cgttcaatac ataggacggg
     6421 taatagccgc atagctgcac atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg
     6481 aacatcacat cgtccgggtt acatgagtag ggatagagcg gcaccatcgc cagcatacag
     6541 ccgactttca tctccgggtt aatgcgacgc gcggctttca ctgccagggc gctggcgaca
     6601 aactggtgat gcagcacctg gtacatcgtc tcttccgggt tgtcatgctc ggtatacacc
     6661 acgccggagc agcagtaacc aaacagcggc gcacgccagt tacgctggtt gtttatttcg
     6721 ttgaaggtca tccagtattt gactttatgc ttgtagcgtt cgaataccac ttccgcgaaa
     6781 cggacaaaga aatcaacgaa tttacggttg gtccagctac cgtattgctg caccagatgc
     6841 agcggcattt caaagtggga gagggttatc accggttcga tgttgtattt cagcagttca
     6901 tcgaacagag agtcgtaaaa cttcagccct tcttcgtttg gctgagcttc atcacctttt
     6961 ggaaaaatac gcgtccaggc gatggaagtc cgaaaacatt tgaagcccat ttcggcaaat
     7021 agcttgatgt cttccttgta gtgaccataa aaatcaacgg cttcatggtt tggatagtat
     7081 tttcctggca agacttcttt ggtgatttcg cgcggcacgc cgtgtgcgcc acaggtcaga
     7141 acgtcacaaa tgctcggtcc tttgccgcct ttgttccagc cgccttcgac ctgatgagcg
     7201 gcaacggcgc caccccataa gaaatctttc ggtaaggtga gttttttcac tatcataggc
     7261 tccagatcag tcaattctta caggaaatag taccatgcag ccaaacgaca tcactttttt
     7321 caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagtct
     7381 gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga tgacggttat
     7441 ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacact gacggaaaaa
     7501 catgcagagc aggaaaatat gaccctgcct gaactgaaaa aggtcattgc tgacatctat
     7561 cccggtcaga cacaatttta tatgattgaa tttaaatgtc tttaaattga actcacgcct
     7621 gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc aaatcagaat
     7681 ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat
     7741 gtgcgtgtac gtttctctgg agtaagttat ggttcagaag cccctcatta agcagggata
     7801 ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggctgg tgtttggtat
     7861 cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg ccacagcgat
     7921 aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta
     7981 tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat
     8041 ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt
     8101 agcgcgcggg ttgatgattg ttatctggag cctagcattg ctggggattc tgttcaaact
     8161 gaccatcgcg caccgattca aaattttatc tctggtgact tatctggcga tgggctggct
     8221 gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttact
     8281 ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca aacgcattcc
     8341 atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc actttctggc
     8401 gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg
     8461 tcagcgtcgc atcaggcatc ggagcgcaac cgccggatgc ggcgtgaacg ccttatccga
     8521 tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga taagacgcgt
     8581 cagcgtcgca tcaggcgata aatgcctgat gcgatacaga gatttactct tcgagcgaat
     8641 acggcagcgg ctcgatatgc agtgtattcg catcgtcgcg tacgcggaag atgctgtccg
     8701 gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg
     8761 cagccagcac cgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac
     8821 cagcttccgg cagtcggctg gcactacctg ccagcaacca gagcgcgcgt ttgttggcac
     8881 cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga
     8941 taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat
     9001 caatcaccgg gaaaccagct tcaatgttta atgccagcca ctgttggcta ttgttcagtt
     9061 ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg gttacgatca
     9121 ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct ttgactacct
     9181 gtttttcttt cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac
     9241 cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg
     9301 catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccatgcaa
     9361 agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg gcgtcgcaat
     9421 gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc acctgaccct
     9481 gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca
     9541 tcagcgtcag cggtaaacgg gcagaagccg taggctgacg tggaggaaaa ggtgtaaaag
     9601 ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa aagagctatg
     9661 aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac tgccaaaaaa
     9721 atgaagccct tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt actgatattc
     9781 ccgtaaaaga agtcgaaaaa cacgttacaa tagccgggta ctggattttc gtgagacagg
     9841 aagaagatgg acattaacaa caaagcccga attcattggg catgccgccg tggtatgcgc
     9901 gaactcgata tttcaatcat gtcgtttttc gaacatgagt acgacagctt aagcgatgac
     9961 gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt taactggctg
    10021 atgaatcacg gtaaaccggc cgatgcagaa ctggaaataa tggtccgact catccagaca
    10081 cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg
    10141 gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccgct
    10201 cagttacacc ccgttatgga tggtgttact ttcgctggtg gtatttgatt gcgttcgcag
    10261 ccagcggtgt attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg
    10321 ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat
    10381 gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat
    10441 ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagata
    10501 aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca
    10561 tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg tcatactggt
    10621 tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc
    10681 tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga
    10741 gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc
    10801 caagcccata cagcgcaata attttacctt caaggttcag gtcgtcgagc tgatcccaga
    10861 cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca
    10921 catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt
    10981 ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac
    11041 cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgcagct
    11101 agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc
    11161 ataaggggcg caagtgaaac aggagctggc acgcatcgag cagtttcttg atgctctgtg
    11221 gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat
    11281 ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca
    11341 ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct
    11401 gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc
    11461 cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc
    11521 gcaggtcgaa cgtttattac aggcgccatt aattgatcag ccactggagc tacgcgataa
    11581 agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac
    11641 aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga
    11701 gcgcctggtg ccgttagggg aagaggcggt ttactggctg gaaacctatc tggaacatgg
    11761 gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca
    11821 gcagatgacg cgacagacct tctggcaccg cattaaacat tatgcagtgc tggcgggtat
    11881 cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc atttattaaa
    11941 tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc tttccaccac
    12001 gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc
    12061 gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac
    12121 tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc aacaaacgtt
    12181 agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg gcatgaagac
    12241 agttctgact aacagcggtg tgttgtacat caccgatgat ggtaaacata tcattcaggg
    12301 gccaatgtat gacgttagtg gcacggctcc ggtcaatgtc accaataaga tgctgttaaa
    12361 gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg aaaaacacgt
    12421 catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc
    12481 agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga
    12541 cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt
    12601 tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg atattgccga
    12661 ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg tgctgagcaa
    12721 tggcacactt gttccgggtt accagccgcc gaaagacatg aaagaattcc tcgacgaaca
    12781 ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt
    12841 gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg ccgtttatac
    12901 gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc
    12961 tggcagcaac taagcggtgt cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa
    13021 ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gcgcgaccag cacggctcta
    13081 agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt accaaaccgt
    13141 ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg
    13201 cagttaattg tcacggtgga taacggtatt tcctcccatg cgggagttga gcacgctcgc
    13261 tcgttgggca tcccggttat tgttaccgat caccatttgc cgggcgacac attacccgca
    13321 gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca
    13381 ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg cgatcagggc
    13441 tggtttgatg agcgcggcat cgcaattcct aacctggcag aactgctgga tctggtagca
    13501 ctggggacag tagcggacgt cgtgccgctg gacgctaata atcgcattct gacctggcag
    13561 gggatgagtc gcatccgtgc cggaaagtgc cgtccaggga ttaaagcgct gctggaagtg
    13621 gcaaatcgcg atgcacaaaa actagccgcc agcgatttag gttttgcgct gggaccgcgt
    13681 ctcaatgctg ccgggcgact ggatgatatg tccgttggtg tggcgctgtt gctgtgcgac
    13741 aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa
    13801 gagatcgaac aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc
    13861 cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca gggcgttgtc
    13921 ggtattctgg cttcgcgtat caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca
    13981 gcaggtgacg gtacgctgaa aggttccggt cgctccattc aggggctgca tatgcgtgat
    14041 gcgctggagc gattagacac actctacccc ggcatgatgc tcaagtttgg cggtcatgcg
    14101 atggcggcgg gtttgtcgct ggaagaggat aaattcgaac tctttcaaca acggtttggc
    14161 gaactggtta ctgagtggct ggccccttcg ctattgcaag gcgaagtggt gtcagacggt
    14221 ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg
    14281 tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct gcaacagcgg
    14341 ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg
    14401 gatggtattg cttttaatgt cgataccgct ctctggccgg ataacggcgt gcgcgaagtg
    14461 caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct gcaaattatc
    14521 atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg tggattgggt
    14581 acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca gaccatgttt
    14641 gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga cgttcttagg
    14701 gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga
    14761 acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc
    14821 cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag atgtttctgg
    14881 tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag ccgttgctga
    14941 actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt tctctggcga
    15001 atatgacagc gccgactgtt acctcgatat tcaggcgggg tctggcggta cggaagcaca
    15061 ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa
    15121 aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg tgacgatcaa
    15181 aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc accgcctggt
    15241 gcgtaaaagc ccgtttgact ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt
    15301 ttatccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc tgcgcattga
    15361 cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg
    15421 tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa
    15481 gaacaaagat caggccatga agcagatgaa agcgaagctt tatgaactgg agatgcagaa
    15541 gaaaaatgcc gagaaacagg cgatggaaga taacaaatct gacatcggct ggggcagcca
    15601 gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgcaccg gggtagaaac
    15661 ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag caagtttgaa
    15721 agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg acgcggtagt
    15781 cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg
    15841 aattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc acgcagaatt
    15901 cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg ttgctggccg
    15961 catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg
    16021 ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca acgagcagtt
    16081 caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac
    16141 cggcgaactg tctatccact gtaccgagct gcgtctgctg accaaagcac tgcgtccgct
    16201 gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga
    16261 tctcatctct aacgatgaat cccgtaacac ctttaaagta cgctcgcaga tcctctctgg
    16321 tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga tgatgcaggt
    16381 gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc tggatctcga
    16441 catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg gtggcttcga
    16501 gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac gtcataaccc
    16561 agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc tgatcgagct
    16621 gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg aagtgaccta
    16681 cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat
    16741 caagaaatat cgcccggaaa ccgacatggc ggatctggat aacttcgact ctgcgaaagc
    16801 gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc gtatcgttac
    16861 cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca ttactgaata
    16921 tccggcagaa gtttctccgc tggcgcgtcg taacgacatt aacccggaaa tcacagaccg
    16981 ctttgagttc ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc
    17041 ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag gtgacgacga
    17101 agcgatgttc tacgatgaag attacgttac cgcactggaa catggcttac cgccgacagc
    17161 aggtctgggg attggcatcg accgtatggt aatgctgttc accaacagcc ataccatccg
    17221 cgacgttatt ctgttcccgg cgatgcgtcc ggtgaaataa gcattacgtt atgctcacaa
    17281 ccccggcaaa tgtcggggtt ttttatttaa gctgggtaaa tgcagataat cgtattctgg
    17341 cttcgcgatt tgtcgcctgc atcaccatcc acggactgaa cgcccacggc gtggcatcaa
    17401 taccgcgtaa tacatcagct aaatcacacc attgataatc catcacttca tcatcgttga
    17461 tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt tccacaatgc
    17521 ctctcggatc ggtggcgcgg tagcgaaagt caggatagat agattcagga ggcgtaattt
    17581 ccacgccaag ctcataacgg caacggcgga tcaccgcgtc ttcgctgctt tctcccagtt
    17641 gtgggtgccc acaaaccgag ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc
    17701 ggcgggtaac taataattgc cccttggcat taaacagcca actggagaac gcgagatgta
    17761 agagggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga actccctgtg
    17821 cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc tgatcactca
    17881 tcattataga taattgaaga gtgaaatgat taaccaagag agcgtttatc gacaaccgcc
    17941 cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca tatatttgaa
    18001 gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga gagtaaaaag
    18061 caacaacaga ctgaatatag cgcgcattaa aaaattcata ttattctcca tgctcatttt
    18121 tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttctaaa tctttatcag
    18181 agacggattc cttcaccagg tcagcatgat gctgatagcc attaaagaag acgttcagca
    18241 caacggcgct aagggttgcg agcataatgc cgctatgtag cagcggttgc agtacggccg
    18301 gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg agactgatcg
    18361 ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga attcgaatcc
    18421 ctgtcgccag taccatgccg aacatcacta gaccagcgcc gcccagcaca aattgcggaa
    18481 tggaggctac cagcaccgcc atttttggca ccatgccaaa caggattaat ataattcccg
    18541 aagaaataca cacccagcgg ctatgaacgc gcatcacgct aaccaggcca acgttttgag
    18601 aaaatgacgt gtgggggaag ctgttaaacg tgccgcctat cattgtccct acgccatcga
    18661 cacgcagccc gcaaataata tcgtgtgaag agagtttacg accgactatt tcacccagtg
    18721 ccaggaacat ccccatcgac tcgataaaca cgatgattaa cacggcagtc atggtcagaa
    18781 tggaaacggg atcgaaaatc ggcataccaa acgacatcgg ggtgacaatc gcaaaccacg
    18841 aggcatcatg tagcccggat aaattgactt cattcatcat ccacgaaagt aaaaagccaa
    18901 atacaatccc cagtaatacg gcgacgttgg acataaatcc tttcgcatag cgagtaatga
    18961 gcaagataaa aattaagacg gcaaaggaga tacctaaata aacgggatta ccatattgcg
    19021 gattcccttt acctccagcg gcccagtcaa tacccacctg aatgatgcta agcccaatag
    19081 aagtaataac cacaccggta accagtggcg ggaataaagg catcaagcga ccaataagtg
    19141 gcgctaataa tgtggtgata aaacccgcgg cgatagtggc accaaatatc cccagcaggc
    19201 cgatatccgg gttcatccca atggctatca ttggtgttac agcagcaaag gtcaccgaca
    19261 taatcaccgg caggcggatc cccataaagc ggccgatacc gatacattgc aataatgtga
    19321 cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc aatagcatct tttgagaggc
    19381 ccagtcggtc accaatcata agaggaacag cgactgcacc tgcgtacatg acaaggacgt
    19441 gttgcagacc gagtatgatc agctttcctg gtgataatat gcggtcaacc tcatcagttg
    19501 ggcggtcttg cccagaagat gagggaagtt gggaatctat ggcgctcatc agtgtttctc
    19561 ctttcgcccg ccagaataaa aagcaaccgt ttacgttaat gaaactgtct aaacacatgc
    19621 tcgtggagtg ccagtttttg cgaggaaggg gagttagaga caggaattgc ggaggcaatc
    19681 acataatcgt gcttaaacca cgaaaatgac aagagaaact gacaatggaa ttatctgtaa
    19741 ttttctcata ttgatatttc attaatcatt ctggcatgtc gcactctcgc atttaatcgt
    19801 ttttatctgg atagcgctct tttgatcggc aatgttgaaa taatgaaagc ggcatagatt
    19861 gaggggcaat aatttgaact cagtcgcaaa attataatta aaacggtcat gctacctaat
    19921 atatgcccgt ttattgcatc gcgaaatttg agaagacata actcattaaa tgaaagggat
    19981 gcaaatgaat acgtttatcg ctgctgaagc tgcggaatgt ataggctgtc a
//
LOCUS       DAZOZX010000071        19998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZOZX010000071 DAZOZX010000000
VERSION     DAZOZX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.071"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(9..221)
                     /gene="cspA"
                     /locus_tag="ACHUPT_002472"
     CDS             complement(9..221)
                     /gene="cspA"
                     /locus_tag="ACHUPT_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0820014.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(502..792)
                     /locus_tag="ACHUPT_002473"
                     /pseudo
     CDS             complement(502..792)
                     /locus_tag="ACHUPT_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1226..1936
                     /locus_tag="ACHUPT_002474"
     CDS             1226..1936
                     /locus_tag="ACHUPT_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0820015.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1986..2960)
                     /gene="ghrB"
                     /locus_tag="ACHUPT_002475"
     CDS             complement(1986..2960)
                     /gene="ghrB"
                     /locus_tag="ACHUPT_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0820016.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3064..3724)
                     /locus_tag="ACHUPT_002476"
                     /pseudo
     CDS             complement(3064..3724)
                     /locus_tag="ACHUPT_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            3877..6075
                     /locus_tag="ACHUPT_002477"
     CDS             3877..6075
                     /locus_tag="ACHUPT_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR0820017.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6044..6484)
                     /locus_tag="ACHUPT_002478"
     CDS             complement(6044..6484)
                     /locus_tag="ACHUPT_002478"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0820018.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6481..7044)
                     /gene="tag"
                     /locus_tag="ACHUPT_002479"
     CDS             complement(6481..7044)
                     /gene="tag"
                     /locus_tag="ACHUPT_002479"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0820019.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7202..7900
                     /locus_tag="ACHUPT_002480"
     CDS             7202..7900
                     /locus_tag="ACHUPT_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0820020.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8129..9337
                     /locus_tag="ACHUPT_002481"
     CDS             8129..9337
                     /locus_tag="ACHUPT_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0820021.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9661..11352
                     /gene="eptB"
                     /locus_tag="ACHUPT_002482"
     CDS             9661..11352
                     /gene="eptB"
                     /locus_tag="ACHUPT_002482"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0820022.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11444..11520
                     /locus_tag="ACHUPT_002483"
     tRNA            11444..11520
                     /locus_tag="ACHUPT_002483"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11478..11480,aa:Pro,seq:cgg)
     gene            12264..13871
                     /gene="dppA"
                     /locus_tag="ACHUPT_002484"
     CDS             12264..13871
                     /gene="dppA"
                     /locus_tag="ACHUPT_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0820023.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14179..15198
                     /gene="dppB"
                     /locus_tag="ACHUPT_002485"
     CDS             14179..15198
                     /gene="dppB"
                     /locus_tag="ACHUPT_002485"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0820024.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15208..16110
                     /gene="dppC"
                     /locus_tag="ACHUPT_002486"
     CDS             15208..16110
                     /gene="dppC"
                     /locus_tag="ACHUPT_002486"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0820025.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16121..17104
                     /gene="dppD"
                     /locus_tag="ACHUPT_002487"
     CDS             16121..17104
                     /gene="dppD"
                     /locus_tag="ACHUPT_002487"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0820026.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17101..18105
                     /gene="dppF"
                     /locus_tag="ACHUPT_002488"
     CDS             17101..18105
                     /gene="dppF"
                     /locus_tag="ACHUPT_002488"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR0820027.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18135..19406)
                     /locus_tag="ACHUPT_002489"
     CDS             complement(18135..19406)
                     /locus_tag="ACHUPT_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0820028.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            19882..19989
                     /locus_tag="ACHUPT_002490"
     CDS             19882..19989
                     /locus_tag="ACHUPT_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0820029.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4769 a         4973 c         5291 g         4965 t
ORIGIN      
        1 agcagagatt acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg
       61 gtgaaggaca ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag
      121 aagtgtacga acacatcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca
      181 gcgttgaacc attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa
      241 ttaagtgtgc ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt
      301 gcactacgag gggtatcaac gataactctt gaagggactt gccttactac actgctttaa
      361 tggtctgtac gtcaaaccgt tgatgtgcat taagccactc attggcgggt gatgcaacaa
      421 ttatttttca tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac
      481 agcaataaag tggataaaag tctatttcat caactgctta cttaatgccg ggttggcttg
      541 aatcaaacgc atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc
      601 ctttaccatg gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaatcctgt
      661 cccttttcat aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt
      721 ctgcgtttca tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc
      781 tttatattcc attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct
      841 cattaagaat agtcgggaaa agcagacaca gcggggtgaa gtcagggcat gctctggcta
      901 tgattcgatt cagcctgtca tttagcgtga cttatagcca cctaacgatc tgataaatgc
      961 cgatatatct cttatcactc ctgttttgta aagatttcgc aaatctaacc ttctgcgaag
     1021 gcgtaaatct ctggctacaa aaaaggcaac ggcactatca ttttttaaca atgaaacaac
     1081 aaattaacct tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat
     1141 cctgcaagcc tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct
     1201 ttttttgggt gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc
     1261 gcctcttgcg gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga
     1321 tcagcgtaaa gcgtttatcg acttcctgca aaatacagtg atgcgtagcg gtgaacgtct
     1381 accaaccctg actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat
     1441 tctgtatggt tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggtcgt
     1501 agacagcgtt aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg
     1561 tgaaatgaat ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga agttgcaggc
     1621 cgatgctgcg cactctgcgc taaaacagag cgatgacctg aaaccggtat ttgatcaggc
     1681 attcaccaaa gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggcggcaca
     1741 aactttcacg caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt
     1801 gagctttgtc gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa
     1861 actgattgcg ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt
     1921 tactgcaacc caataaagag taaaagcccg agcgattatc gggctttact tcacgcagtc
     1981 gcagcttagt ccgcgacgtg aggattcaca cagttcttct caacctttcc ttgtaacgca
     2041 tcaatcaaat tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat
     2101 ccaatatgcg gtactgcgac gacgttggcc attgagagca acggcgaatc tacggacagt
     2161 ggctcttgtt cgaagacatc cagcccggcg gcgtgaattt cgcctttctg caatgctgcg
     2221 atcagtgcat tttcgtcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat
     2281 ttcattttgg cgaattgttc tgcgccaaac agatgatgcg tctcatcagt taacggcagg
     2341 atcaggcaaa cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg
     2401 aagcgttctt ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag
     2461 ccaaagtgca cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt
     2521 gttttatggt gaacgtcagt gccgtaccag tccgggccta tgctcgcggt ccattcgcct
     2581 gcttttaccc gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc
     2641 agcgtatcgg cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg
     2701 gcggtaagcg catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc
     2761 agtttcggca ttttttccag caatgcggca tctacattct cgtttgaacc cagtaaacct
     2821 tcagcttctg caaaaattgc tgcattttga acgacggttt gtgggctgag gtttgccacc
     2881 tggtgaacgg tgaaatgcgc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag
     2941 aggataacgg acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg
     3001 aaatatgatg aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag
     3061 ggattacagc gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt
     3121 gctgttgctg gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc
     3181 cacgccctgg gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat
     3241 gttcaggtcg tgaccccccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg
     3301 atactctttc agcaccattg ccacgccggt cagggtgtta gcgcctgccg gtttcagggg
     3361 agcgctgctg ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct
     3421 gcgggttacg ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc
     3481 catgtaataa cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa tcagcgcgcc
     3541 tttaccgcga tctttcttcg aagaagagag cgcaccaata cccgcgccca cgagagagcc
     3601 cagacctgcg ccgatagcag atttgcctgc ttcgcgttcg ccggtgtaag ggttagttgt
     3661 gcagccagat accgccagag cgccactcac tacggcggca agtaagataa acacgtttct
     3721 tcattgttaa tccttaataa cctttttatt ctttgtcacg ggttccgtgg cgggagatta
     3781 tgccgcgtga acatgaagat tattcctggg aatactcgga aatttgtaag taatatttaa
     3841 ctgctcaata catctaacct ttcaggagtc ttcggtttgg ccaactcatc ctcacgatat
     3901 tccgttctga ctgccgccca ctgggggccc atgctggttg aaaccgacgg cgaaaccgtg
     3961 tttagctcgc gtggcgcgtt agccacagga atggaaaact ccttgcagag cgcggttcgc
     4021 gaccaggttc acagcaatac gcgggtacga tttccaatgg tgcgcaaagg ctttctcgcg
     4081 tcaccggaaa acccgcaagg cattcgtggg caggatgaat ttgttcgcgt gagttgggat
     4141 gaggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4201 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4261 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4321 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4381 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4441 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     4501 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     4561 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     4621 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     4681 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     4741 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     4801 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtgaccccac gcggcgttct
     4861 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     4921 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     4981 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5041 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5101 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5161 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5221 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5281 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5341 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5401 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     5461 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     5521 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     5581 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     5641 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     5701 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     5761 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     5821 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     5881 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     5941 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6001 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6061 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6121 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6181 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6241 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6301 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6361 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6421 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     6481 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     6541 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     6601 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     6661 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     6721 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     6781 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     6841 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     6901 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     6961 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7021 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7081 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7141 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7201 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7261 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7321 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7381 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7441 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     7501 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     7561 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     7621 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     7681 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     7741 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     7801 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     7861 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     7921 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     7981 tgaataattg tcgcgtcatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8041 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8101 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8161 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8221 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8281 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8341 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8401 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     8461 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     8521 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     8581 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     8641 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     8701 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     8761 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     8821 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     8881 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     8941 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9001 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9061 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9121 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9181 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9241 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9301 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9361 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9421 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
     9481 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
     9541 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
     9601 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
     9661 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
     9721 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
     9781 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
     9841 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
     9901 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
     9961 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10021 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10081 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10141 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10201 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10261 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10321 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10381 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10441 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    10501 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    10561 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    10621 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    10681 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    10741 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    10801 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    10861 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    10921 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    10981 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11041 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11101 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11161 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11221 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11281 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11341 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11401 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    11461 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    11521 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    11581 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    11641 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    11701 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    11761 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    11821 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    11881 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    11941 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12001 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12061 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12121 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12181 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12241 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12301 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12361 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12421 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    12481 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    12541 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    12601 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    12661 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    12721 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    12781 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    12841 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    12901 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    12961 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13021 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13081 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13141 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13201 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13261 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13321 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13381 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13441 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    13501 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    13561 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    13621 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    13681 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    13741 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    13801 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    13861 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    13921 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    13981 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14041 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14101 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14161 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14221 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14281 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14341 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14401 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    14461 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    14521 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    14581 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    14641 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    14701 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    14761 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    14821 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    14881 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    14941 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15001 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15061 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15121 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15181 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15241 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15301 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15361 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15421 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    15481 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    15541 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat
    15601 aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    15661 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt
    15721 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    15781 tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc
    15841 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    15901 ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg
    15961 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    16021 cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    16081 gacgcgctcg atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga
    16141 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    16201 ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc
    16261 ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa
    16321 actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    16381 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    16441 cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg
    16501 ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct
    16561 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    16621 gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac
    16681 cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct
    16741 ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat
    16801 gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca
    16861 cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct
    16921 ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa
    16981 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    17041 cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact
    17101 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    17161 cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc
    17221 gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt
    17281 aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat
    17341 tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    17401 atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa
    17461 attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa
    17521 gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac
    17581 atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg
    17641 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    17701 ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac
    17761 gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc
    17821 gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg
    17881 ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc
    17941 ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt
    18001 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg
    18061 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    18121 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    18181 taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag
    18241 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    18301 aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    18361 cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac
    18421 agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg
    18481 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc
    18541 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    18601 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata
    18661 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt
    18721 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa
    18781 aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata
    18841 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    18901 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    18961 atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    19021 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    19081 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    19141 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    19201 aaataaatgt cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat
    19261 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    19321 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg
    19381 tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    19441 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    19501 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    19561 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa
    19621 attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagcaa
    19681 tcgtagaatc agcgtcaagc cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa
    19741 tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg tatgtacctg
    19801 acaacataac gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt
    19861 cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    19921 ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag
    19981 cggaagtagc gtgtcatg
//
LOCUS       DAZOZX010000072        19698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZOZX010000072 DAZOZX010000000
VERSION     DAZOZX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.072"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUPT_002491"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUPT_002491"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0820030.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUPT_002492"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUPT_002492"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0820031.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUPT_002493"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUPT_002493"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0820032.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHUPT_002494"
     CDS             6038..6658
                     /locus_tag="ACHUPT_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0820033.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHUPT_002495"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHUPT_002495"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0820034.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHUPT_002496"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHUPT_002496"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0820035.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHUPT_002497"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHUPT_002497"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0820036.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHUPT_002498"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHUPT_002498"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0820037.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHUPT_002499"
     CDS             complement(10068..11069)
                     /locus_tag="ACHUPT_002499"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0820038.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHUPT_002500"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHUPT_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHUPT_002501"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHUPT_002501"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0820039.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHUPT_002502"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHUPT_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0820040.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHUPT_002503"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHUPT_002503"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0820041.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHUPT_002504"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHUPT_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0820042.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHUPT_002505"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHUPT_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0820043.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHUPT_002506"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHUPT_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0820044.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZOZX010000073        19507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZOZX010000073 DAZOZX010000000
VERSION     DAZOZX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.073"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(89..1318)
                     /locus_tag="ACHUPT_002507"
     CDS             complement(89..1318)
                     /locus_tag="ACHUPT_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0820045.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGEKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            1567..2688
                     /gene="roxA"
                     /locus_tag="ACHUPT_002508"
                     /gene_synonym="ycfD"
     CDS             1567..2688
                     /gene="roxA"
                     /locus_tag="ACHUPT_002508"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0820046.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(2737..3963)
                     /gene="pepT"
                     /locus_tag="ACHUPT_002509"
     CDS             complement(2737..3963)
                     /gene="pepT"
                     /locus_tag="ACHUPT_002509"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0820047.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            4213..5349
                     /gene="potA"
                     /locus_tag="ACHUPT_002510"
     CDS             4213..5349
                     /gene="potA"
                     /locus_tag="ACHUPT_002510"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0820048.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            5333..6190
                     /gene="potB"
                     /locus_tag="ACHUPT_002511"
     CDS             5333..6190
                     /gene="potB"
                     /locus_tag="ACHUPT_002511"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0820049.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            6187..6981
                     /gene="potC"
                     /locus_tag="ACHUPT_002512"
     CDS             6187..6981
                     /gene="potC"
                     /locus_tag="ACHUPT_002512"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0820050.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            6978..8024
                     /gene="potD"
                     /locus_tag="ACHUPT_002513"
     CDS             6978..8024
                     /gene="potD"
                     /locus_tag="ACHUPT_002513"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0820051.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(8180..9001)
                     /gene="cobB"
                     /locus_tag="ACHUPT_002514"
     CDS             complement(8180..9001)
                     /gene="cobB"
                     /locus_tag="ACHUPT_002514"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0820052.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(9017..9928)
                     /gene="nagK"
                     /locus_tag="ACHUPT_002515"
     CDS             complement(9017..9928)
                     /gene="nagK"
                     /locus_tag="ACHUPT_002515"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0820053.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(9957..11201)
                     /gene="lolE"
                     /locus_tag="ACHUPT_002516"
     CDS             complement(9957..11201)
                     /gene="lolE"
                     /locus_tag="ACHUPT_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0820054.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(11201..11902)
                     /gene="lolD"
                     /locus_tag="ACHUPT_002517"
     CDS             complement(11201..11902)
                     /gene="lolD"
                     /locus_tag="ACHUPT_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0820055.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(11895..13094)
                     /gene="lolC"
                     /locus_tag="ACHUPT_002518"
     CDS             complement(11895..13094)
                     /gene="lolC"
                     /locus_tag="ACHUPT_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0820056.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            13356..14429
                     /locus_tag="ACHUPT_002519"
     CDS             13356..14429
                     /locus_tag="ACHUPT_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0820057.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            14557..18003
                     /gene="mfd"
                     /locus_tag="ACHUPT_002520"
     CDS             14557..18003
                     /gene="mfd"
                     /locus_tag="ACHUPT_002520"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0820058.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            18146..19108
                     /gene="ldtC"
                     /locus_tag="ACHUPT_002521"
     CDS             18146..19108
                     /gene="ldtC"
                     /locus_tag="ACHUPT_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR0820059.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(19191..19403)
                     /locus_tag="ACHUPT_002522"
     CDS             complement(19191..19403)
                     /locus_tag="ACHUPT_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR0820060.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4694 a         5143 c         4900 g         4770 t
ORIGIN      
        1 tcgtagttgt tccgcattgt tgctcattgt tgcacgttgt atctgttcgc atatccagta
       61 tgcgcatact gaaaaaacac gaaaaaaatt attttcttct ggctactggt agcgtggtta
      121 cgttttcatg tgttcccgcc agtatcccta accgctccgt ccacatatcc agcgcattgc
      181 gtttagcatc cagataacgg gaatgattat aaactcgctg cattcctggc atctggtggc
      241 ctgtaagctg ctccacgacg tgaggatcca cgcctaaatc gttcagcatc gttgtaaagg
      301 tgcgccggat gtcatgtagt gaccagtgag ggtgatttag cctcctgtgc gctaatctgc
      361 cgtactgcga cacgcttgtt tcctgtttca cttcccccag caataagccc gtgtgcctgt
      421 tctgctccac cagctgcgtg acgaacggca gtattgcttc cggtatgggc cggaatattg
      481 ccaccttcgt tttgctgtgt tctttcggca cggtccagag catttcggta aaatcccact
      541 cgctgatctc cgataacctc agttctaccg tccggcatcc gaacacaatc aggaggcgga
      601 ttaacgcgat gtagtagggg gagaatattt ttttgtccag tgcctgcaat aattcgccca
      661 gttctttggt gcttaagaca cgctcgctta tatccggttt ttccccaacg tccgccacac
      721 tcatatcatc aagaacgttg ctgattgcat agcgccgcct ccggcagaac ttaagcgcct
      781 gtttgcacgt ctgtagcaag aatccggcag taacaggcgt tcgctttgcc acctggtcaa
      841 aacaggccag ccagtgccgt agctcgcatt tatccagcgg catagcacca atgtgctgta
      901 ttacgtgatt attaaggcgc tttttcaggg cggcataatc cacgcggttt tcctttgcgt
      961 acgactcaag ccagtaggtg agcgcatcgc caaccgttac cggctttaac gcttcctgta
     1021 cggtgtaatt aagctcatga cgtggatttt tcccctctgc cagccatgcg cgacactggg
     1081 cggctttttc cctggctgat ttcaggctca gatcaggata atttcccagc ttaatgcgtt
     1141 ccggtggtgc cccccttccc gttccggccc tgtaagtgaa ataccaggtt aaaaggccac
     1201 tggttgaatg cctgacgctc aggtttccac cgtcattaag aaaggctgtt ttttgggcgg
     1261 gtgtgccgtt gattttcctc agctgtgtat cgctcagttt gttaagtgct ctgctcataa
     1321 ttttgattcc ggtcatcaca attaactaca ccattaacta caccgatcgt tgcacaaagg
     1381 gctacaacgt gaaacaagct ggaacaagga aaatcacaaa tctgtttata atcaaaaaca
     1441 taatgaacaa tctgaaacat tatgaaacgg caaaaaacac taattgataa aatatgtcca
     1501 tacttcacgc attacgttaa gcatccgtta taatcggttg cagataccag cctgtggatg
     1561 cttaacatgg aataccaact cactcttaac tggcccgatt ttcttgaacg tcactggcag
     1621 aaacgcccgg tggtgttaaa acgcggcttt aataatttta ttgacccgat ctctccagac
     1681 gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc gactggtcag tcaccaggat
     1741 ggcaaatggc aggtcagcca cggcccgttc gaaagctacg atcatctcgg tgaaaccaac
     1801 tggtcattac tggtacaggc agtaaaccac tggcatgagc cgaccgccgc gctgatgcga
     1861 ccgttccgtg aactaccgga ctggcgtatt gatgatctga tgatttcttt ttctgtacct
     1921 ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt ttatcattca gggtaccgga
     1981 cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac agcactgccc acatccggat
     2041 ctgttacagg tcgatccgtt cgaagccatc atcgatgaag agctggagcc tggtgatatt
     2101 ctttatattc cgccaggatt cccgcatgaa ggctacgcgc tggaaaatgc gatgaactat
     2161 tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta gcggatttgc cgattatgtg
     2221 ctgcaacgtg aactgggcgg caactactac agcgatccgg atgttccacc tcgtgctcat
     2281 cctgcggatg ttctgccgca agagatggat aaactgcgtg agatgatgct cgaattgatc
     2341 aaccagccgg aacactttaa gcaatggttt ggcgagttta tatcccagtc acgtcatgaa
     2401 ctggatatcg cgccgccgga accgccttat cagccagatg aaatctacga tgcgctgaaa
     2461 caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat tgcgcattgg cgacgacgtg
     2521 tatgccaatg gtgagaagat cgattccccg caccgtccgg cactggatgc actcgccagc
     2581 aacattgcgc tgactgcgga gaattttggc gatgcgctgg aagatccgtc attcctcgcg
     2641 atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg aagggtaagt ttggatttaa
     2701 gccggatgca gcatcacatg ccgcatccct tttcgcttac ttccgttgcg ccgttaactc
     2761 ggcaatacgg acgatcacct gcaccgcttt ttccatacct tccagaatca caaactcatg
     2821 cttaccatga tagttgtaac cgccagtgaa caggttcggg caaggtaatc ccataaacga
     2881 caactgcgcg ccgtcggtac cgccgcggat cggtttcagt tccggttcaa tatcgcagtc
     2941 acgcatcgcc tgctgggcga tatcgagaat atgcggatgc tcaaccactt tctcgcgcat
     3001 attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa tcaggatgta accctttgcc
     3061 cacttttttg gcgatctcca tcattttacg tttacgcgct tcaaactgtt tacggtcgaa
     3121 atcacggatg atgtagtgca tatcggcccg ttcaacggtg cctttcatgc tcgccaggtg
     3181 atagaaacct tcatagcctt ctgtcatttc cgggctttca tccgccggaa cttccgcatg
     3241 aatacgtgcc gccagcgaca gcgcatttac catcactcct ttcgccgtgc ccggatgaac
     3301 attgttaccg acaattttta tattgaccga tgcggcgttg aagttttcaa actccagttc
     3361 gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca tcgaaggcat caacatcaaa
     3421 atgtttcgcc cctttgccca cttcttcatc cggggtaaag gcgacgcgaa tatcaccatg
     3481 cggaatgttt ttctgttgca ataccgccag cgcggtcatg atttctgcaa tacctgcttt
     3541 gtcatcagca cctaacaagg ttttaccatc ggtggtaatc aacgtctgac ccagtagctg
     3601 atgcagcacc gggaacataa ccggtgataa aacttcatcg ccgataccca gcgcaatatc
     3661 gccaccgcga tagttttcaa caatttgcgg attaacattt ttgccgctgc aatccggtga
     3721 ggtatccaca tgagaaataa agccaatcgc cgggatatcg ccagggacgt tagccggtaa
     3781 cgtcgccatc aaagtgccct tctcacttaa ggtcacattg ataagcccca tttcttcgag
     3841 ctgctctttc agcagatgca ataacttcca ttggccttcc gtgctgggaa cctgtctcac
     3901 ccctgctttt gattgggtat ccagagacac gtagttcaaa aatcgctcaa gtagtttatc
     3961 catgtagtca ccctcacttt ttgtgacaac attattaaga agcaagaaaa gacaaatatt
     4021 gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat cagcattacc ttaatgaata
     4081 taaagctaag ccagtaattc acaacaaaga ttggcgattc aagcggtttg ccgtagaatt
     4141 accgccctca ttaggagtca ccaaggtggt taaccacaaa ccccgcatcg gtaagccatc
     4201 cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac aaccgagttc gctttcaccg
     4261 ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta aagaggtcat tccccagctg
     4321 gatctgacta tcaacaatgg cgagttcctc acgctgcttg gcccttctgg ctgcggtaaa
     4381 acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg attccggacg catcatgctg
     4441 gataacgagg acatcaccca cgttccggcg gaaaaccgct atgtgaacac tgttttccaa
     4501 agctatgcac ttttccccca catgaccgtg ttcgaaaatg tggcctttgg gttgcgcatg
     4561 caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg aagccctgcg gatggtgcag
     4621 ctggaaacct tcgctcaacg taaaccgcat cagctctctg gtggtcaaca gcaacgcgtc
     4681 gccattgctc gagcggtggt taacaagcct cgtctgttgt tgctggatga gtcgctctca
     4741 gcgctggatt acaaactgcg taagcaaatg cagaacgagc tgaaagcgtt acagcgtaag
     4801 cttggcatta cattcgtttt cgtgactcac gaccaggaag aagcactcac catgtcggac
     4861 aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg gcacgccgcg tgaaatctac
     4921 gaagagccga aaaacctgtt tgttgccggg ttcattggcg aaattaatat gtttaacgcc
     4981 acggtgatcg agcgactcga cgagcagcgc gtacgcgcca acgttgaagg ccgcgaatgt
     5041 aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac tgcatgttct gctgcgcccg
     5101 gaagacttac gtgttgaaga gattaacgac gacaaccacg ctgaagggct gattggttac
     5161 gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg ttgttgaact ggaaaatggc
     5221 aagatggtga tggtcagcga attcttcaat gaagacgatc ctgactttga ccactctctc
     5281 gaccaaaaaa tggccattaa ttgggtagaa agctgggagg tcgtactggc tgatgaagaa
     5341 cacaagtaag ttccagaatg tagtgattgt cactattgtc ggttggcttg tgttgtttgt
     5401 ctttctgccc aacctgatga tcattggcac cagctttttg acccgcgacg acgccagttt
     5461 cgtcaaaatg gtctttacac tggataacta cacgcgtctg ctcgatccgc tctattttga
     5521 agtgctattg cactcgctga atatggcgct gatcgccacc ctcgcctgcc tggtgctggg
     5581 ctatccattt gcctggtttc tggcgaagct gccacacaag gtgcgtccgc tgctgctgtt
     5641 tctgctgatt gttccgttct ggaccaactc attgattcgt atctacgggc tgaaaatttt
     5701 cctcagcacc aaaggctatc tcaacgagtt tttgctctgg ctgggcgtta tcgacacacc
     5761 aatccgtatc atgttcacgc ccagtgcggt gattatcggt ctggtttaca ttctgctgcc
     5821 gtttatggtg atgccgctgt actccagtat cgaaaaactg gataagccgt tgcttgaggc
     5881 ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc cgcatcatta ttccactgac
     5941 gatgccgggg attattgccg gatgtctgct ggtgatgctg ccagcgatgg gcctgttcta
     6001 tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc ggtaacgtca tcaaggtaca
     6061 gttcctcaat attcgtgact ggccgtttgg tgcagccacc agcattacgc tgactatcgt
     6121 aatgggcctg atgttgctgg tttactggcg cgcttctcgt ttgctgaata agaaggtgga
     6181 actcgaatga tcggtcgact gcttcgcggc ggttttatga ccgctatcta cgcgtacctg
     6241 tatatcccaa tcattatttt gattgtgaac tcctttaaca gctcgcgctt tggcatcaac
     6301 tggcagggtt ttaccaccaa atggtatagc ctgctgatga acaacgacag cctgttacag
     6361 gcagcgcagc attcactaac aatggcggtg ttttcggcga cgtttgctac gcttatcggt
     6421 tcactgacgg cagttgcgct gtaccgttat cgctttcgcg gtaagccgtt cgttagcgga
     6481 atgctgtttg tggtaatgat gtcaccagat atcgtgatgg caatttcgct gctggtgctg
     6541 tttatgctgc tgggtattca gcttggcttc tggtcgctgc tgttctcgca tatcaccttc
     6601 tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga aaggttttga cgtgcggatg
     6661 ctggaagcgg cgaaagatct cggtgccagc gaatttacca ttctgcgaaa aatcattctg
     6721 ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa gctttaccct gtcgatggac
     6781 gatgtggtag tttcttcatt cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt
     6841 tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg cgctggcaac catattactg
     6901 gtgctgtcgc tggtgatggt tattgccagc cagcttattg ctcgtgataa aacgaaaggt
     6961 aacacagggg acgttaaatg aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg
     7021 cactgggcat gagcgccgct cacgccgatg acaacaacac gctgtatttc tacaactgga
     7081 ccgagtacgt gccgccagga ctgcttgaac agttcaccaa agaaaccggt attaaggtta
     7141 tctattcgac ttacgagtcg aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg
     7201 gtgcctatga cctggtggtt ccttcaacct attacgtcga taaaatgcgt aaagagggga
     7261 tgatccagaa gatcgacaag tcgaagttaa cgaacttcag caatctcgat ccagacatgc
     7321 tcaacaagcc ttttgacccg aataacgact actccattcc gtatatctgg ggtgcgacgg
     7381 cgattggtgt taacggtgat gcggtggatc cgaaatctgt caccagctgg gccgatctgt
     7441 ggaagccaga gtacaaaggc agcctgctgt tgaccgacga tgcccgtgaa gtgttccaga
     7501 tggcgctgcg taagctgggc tactccggta acaccaccga tccgaaagag attgaagctg
     7561 catataacga gctgaaaaaa ctgatgccaa acgtcgcagc gtttaactcc gataacccgg
     7621 ctaacccgta catggaaggc gaagttaacc tcggcatgat ctggaacggt tctgctttcg
     7681 ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc gaaagaaggc ggcatattct
     7741 ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa agaaggcgcg ctgaaattga
     7801 tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc tgaaactatc ggttatccaa
     7861 cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt ggcaaacgat aagacgctct
     7921 acccggatgc tgaaaccatt aaaaatggcg aatggcagaa tgacgttggc gcagccagca
     7981 gcatttatga agagtattat cagaagctga aagcaggacg ttaattcagc aagcgggcgg
     8041 tggtattacc gcccgttttt tagttagcaa ggatgtggct aatgagtcag gacagtaaag
     8101 ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta ggcctgataa gcgtagcgca
     8161 tcaggcaatg cttcctgctt tataatccct ccagcaactt ttcaacaaat tctggcacca
     8221 cctggcttgc cgggccgtaa tatttctcgg caaattcatt accaacctga ctcggttcaa
     8281 gattcagttc aacggtgtga gcaccatgta gtttggcttc gtgtacaaac ccagccgccg
     8341 gatagacatg cccggaagta ccaatggcaa taaagacatc cgccatcgac aacgccatat
     8401 aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg
     8461 ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc cagtcgagaa
     8521 cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga atgacattgg
     8581 tattacctgc gcgttcatgc aggttgtcta tattctgcgt taccagcaaa aagcgatcgc
     8641 cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga atttctggct
     8701 gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga tcgcgatcga
     8761 aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc
     8821 gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc tgtcagtacg agtactcttg
     8881 gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga cgcaaacgct
     8941 cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg atgaccccga cgcgacagca
     9001 tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc attcctcccg
     9061 catcaccgtg gcgcgcgcgt tcaatgcgtg gaacacgagc tacaggtaag agatgacaag
     9121 gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca ccaccaatga
     9181 cgaccaggtc agggtcaaca atggtcagga tatttcccag agaaaccgct aataaatcca
     9241 gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc gcaataattt
     9301 cgggagcctg caacggttga tgataatagt gttgatacag ccacgcaaaa ccgcgaccag
     9361 acagataatt ttcaatgcag ccatgctgac cacagccgca gcggcgtaac gggaaatcca
     9421 accccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg ccggtaatgt
     9481 agcttttacc agtaatcggt ttgccgttga aaaccagccc gccgccaacg ccggtgccga
     9541 gaattaaccc catcaccagt ggatattgcg taaattcatc atcccaggct tctgaaaggg
     9601 caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg
     9661 cacgcagcgg tttgccgctg gcagcgggga cattagcggc atacagctta ccatcttctg
     9721 tttccggcat tcccggaata ccgatgccga cagaaccttt acagccaaaa cgttggtcag
     9781 cttcagctac cagctcacac actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg
     9841 gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc gcaatttttg
     9901 ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta ctgcctttac
     9961 tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg ataccaactt
    10021 gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag ccagtgcaat
    10081 tccgatggca ggaagtcaat aaaataaata tcgctggaga ggaactgatg accgatcagc
    10141 ttttcaatcc actcaataat cggggtaagt tgcagtgaaa caactacgcc gataaccacc
    10201 ccacacaggc tgccgaacag ccctgccagc aatccatacc agacaaagat ggcgcgaatt
    10261 aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt gtctttcacc
    10321 gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag taccatcgcc
    10381 agatacataa tggcgcggat catctggata tcgcgataca tatagccgta agtaccaatc
    10441 caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac cagcttattg
    10501 gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc catatcaaga
    10561 tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg accactcaac
    10621 tgcaaaatac cggcaatgtg caaacgcaca cgttttggct gcatcagttt atgctcggga
    10681 ttcgagttgg ggatcatgat cgacacccaa tcgccctgct tcactttcag cgcatccgcc
    10741 acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca cgcatccccc
    10801 tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt gtgggttaac gcccttcacc
    10861 tggattgcgc gcagattcgc tccactttcc accagaccgg tgaaattgat atacggcgca
    10921 gcggcggcaa tacccggcac tttctgcacg ttatccagtg cttcctgcca gttagtccat
    10981 ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg ccagaatgcg gttattcagt
    11041 tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac gccgagggca
    11101 atgccaatgg tagaaatgac agagattagc gacaccatgc cgccgcgtcg ccgtccgcga
    11161 ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca ttactccgcc cccatcaggc
    11221 tcagttccgc cgtcagacgt ccatcacgca tttccagttg gcggctcata cgtttcgcca
    11281 gttgcaggtc gtgagtgacc accaggaaag cggtgccctg caagcgattc aactccccaa
    11341 gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggt tcatccgcca
    11401 gtaccagacg cggattattg accagcgcac gggcaatcgc tacacgctgg cgttcgccgc
    11461 cagaaagttc agatgggcgg tgattcgcac gatgctccag ccccaccgct tttaacatct
    11521 ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc aatcagcagt ggcatagcca
    11581 cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa ctgataaata aagcccagct
    11641 tctggttgcg cagttctgct ttcgccgctg aagacagttt gctcatcggc tgaccgttaa
    11701 agatcacatc gccggaggtt ggcgtatcca gcccgcctaa caggtgcagc aaggtacttt
    11761 taccggaacc agagctaccg acaattgcca tcatttcacc ttcgcccacg ctgaaactga
    11821 cattgagcag cacatcggtt tgcacactgc cttcctgata gcgtttgcac aggttgtcgc
    11881 attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgtgtggc ggcagcgcgc
    11941 catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc aataacaatg
    12001 acctgtaaag gttcgatagc caccggcagc gccgcgccat caagcaggac gccgattatc
    12061 ggcatcagat tatttaactg gctggcaagc agtgcgccaa gcgccgctcc gaggattgca
    12121 ccgataatcc cggcgctagc cccttgcacc ataaagacca tcatgatttg tcgcggagtt
    12181 aagccttgcg tttgcaggat cgcgacttcg ccctgcttct ccatcaccat cagccccagc
    12241 gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc tcagcagtaa gcccatcata
    12301 tttttttcca tgcgtacggc ctggaacagc tcgcctttac ggtcacgcca gtcctgccat
    12361 ttgctgcctt caggcagttt ttgctgactt aaagagtcaa ctctcagcgg ctcatccagc
    12421 cacaaacgcc agccggtaat attggctgcc ggataacgca tcagacgcga ggcatcctca
    12481 atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt gccaatcaca
    12541 ttgaacaggc gctgacttgg aatacgcccc atcggcgtga actggctggc agatggcacc
    12601 atcacgcgga tttgatcacc gcgattgacg cccaactgtg aggcaagttg ttcgccgagg
    12661 atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag atacggcgtt
    12721 agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac gctgcgcgcg
    12781 ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg
    12841 actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga gagaattgcc
    12901 tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt catcactgac
    12961 aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag ccaggagacg
    13021 aaacgaccga agcgatccac tgcacgccca cgcatgtaac gcaggccaat aaatagagcg
    13081 acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta tataagcgaa
    13141 agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg aaaagccccc
    13201 gtaaatcaat cggctaaatt gccatatttt atcatcaatc ttgccgcctg atcttaatct
    13261 gaaaaatcat ccaatacaga cagataccca ttacacctcg ggtcataacc agcaggatca
    13321 accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa acaaaaagag ctatggatta
    13381 accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc attacctttt
    13441 atccgcatat gaccactttt cagcatccgt tatcggaagt cctgagcaaa tgctggatct
    13501 atttcaatct ttaccttgcc ccctttcgta tgccggtttt tttctttata tccggctatt
    13561 tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa cggatctgga
    13621 acatcttctg ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta agtgcgctga
    13681 accagtggct ggcacctgag cgcgatttaa gcaatgcctc caatgccgct tatgccgatt
    13741 ccaccggtga gttcctgcac gggatgatca ccgccagcac cagcttgtgg tatctgtatg
    13801 cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg ccactattcg
    13861 ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg tggggaatga
    13921 acagtgtgat acgcaatttg ccttattaca gccttggcgc atggtttggc gcaacaataa
    13981 tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct ttgcttaccg
    14041 ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg gtatcgattg
    14101 tggtgatcat gaagctgttt tatcagtacg agcaacgatt cggtatgcgc tccaccagcc
    14161 tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc attctggttg
    14221 aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg
    14281 aactgacact cctgctggtt tatccctttg tcagtttgtt catctgtact gttgcgggct
    14341 tgctggtaag aaaactttca cagcgtgcat tcagcgatct gttgttctcc ccgccttcac
    14401 tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc agccggggcg attcaatttg
    14461 ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat gcccccatat
    14521 gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata tcgttatacg
    14581 ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca
    14641 acgctggtag cggaaattgc cgaacgtcac gccggtccgg tggtactcat tgcaccagat
    14701 atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt tcaccgatca gatggtgatg
    14761 aatctggcgg actgggaaac tcttccctac gacagttttt cgcctcatca ggacattatc
    14821 tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcat actgattgtt
    14881 ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg
    14941 gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac gaacccaact ggacagcgcc
    15001 ggttatcgcc atgttgacca ggtgatggag cacggcgaat acgccacgcg cggcgcgttg
    15061 ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa
    15121 atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga agtagaagcg
    15181 atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga actgttccgc
    15241 agccagtggc gcgatacctt cgaagtgaag cgcgatccgg aacatattta ccagcaagtg
    15301 agtaaaggca cattacctgc tgggatcgag tactggcagc cattgttctt cagcgaacca
    15361 ctgccaccgc tgttcagtta tttccctgcc aataccttgc tcgtgaatac tggcgatctg
    15421 gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc
    15481 gatccgatgc gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga cgagctcttc
    15541 tcagagctga aaaactggcc ccgagtgcag ctaaaaactg aacatttacc gacaaaagcc
    15601 gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc gcaacaaaaa
    15661 gcgccgctgg atgcgctgcg taagttcctc gagactttcg acggtccggt ggtgttctcg
    15721 gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct
    15781 ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct gatgattggc
    15841 gctgccgaac atggttttgt cgataaggcg cgtaatctgg cgctgatctg cgaaagcgat
    15901 ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc gccgcaccat caaccccgat
    15961 acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca tctggagcac
    16021 ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg gcggcattac tggcgagtac
    16081 ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg
    16141 attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact tggcggcgat
    16201 gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg
    16261 ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag
    16321 cagtatcagt tattctgcga cagcttcccg tttgaaacca cgccggatca ggcgcaggcc
    16381 attaatgcgg tacttagcga catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc
    16441 gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc agtagataac
    16501 cacaagcagg tagcggtgtt ggtgcctacc acccttcttg cgcagcagca ttacgacaac
    16561 ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg tttccgcagc
    16621 gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga tattctgatc
    16681 ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct gctgattgtc
    16741 gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac
    16801 gtggatattc tgacgcttac tgcaacgccg atcccacgta cgctgaatat ggcaatgagc
    16861 ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc
    16921 tttgtccgtg agtatgacag cctggtggtc cgggaggcga tcctgcgtga aattttgcgc
    16981 ggaggacagg tttattatct ctacaatgat gtggaaaaca ttcagaaagc cgccgaacgg
    17041 ctggcagaac tggtgcctga agcacggatt gccatcggtc acgggcagat gcgcgagcgc
    17101 gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct ggtttgtacc
    17161 accattatcg aaaccgggat cgacatcccg acagccaaca ccattatcat tgaacgtgcg
    17221 gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg ttcacatcat
    17281 caggcatatg catggctgct gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa
    17341 cgtcttgaag cgattgcctc gctggaagat ctcggggcag gttttgcgct ggcaacgcac
    17401 gatctggaga ttcgcggcgc gggtgaactg cttggcgaag aacaaagcgg ctcaatggaa
    17461 accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc
    17521 ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga gctgcggatg
    17581 ccgtcgctat tgccagatga tttcatccct gacgtgaaca cgcgtctgtc gttctacaaa
    17641 cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat
    17701 cgcttcggcc tgctgccaga tccggcgcgt accctgctgg atattgcccg tctgcgccag
    17761 caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa
    17821 tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca aaaacagccg
    17881 cagcattacc gccttgatgg tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg
    17941 aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa cgcgatcgct
    18001 taatgtaaat cgggggcgtt tctgcgcccc catctgcaac atttacaaat tctttgcact
    18061 ttcctgcact atccgacact gtcaccatcc ataattcagg cttatctgtt tattacaata
    18121 accttatatt tattatgtat ttttggtgat gatcaaaacg cgtttttctc gctggctaac
    18181 gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg gcaaaagcca acacctggcc
    18241 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    18301 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    18361 agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    18421 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    18481 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    18541 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    18601 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    18661 gcctgcggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    18721 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    18781 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    18841 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    18901 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    18961 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    19021 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    19081 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    19141 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    19201 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    19261 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    19321 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    19381 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag gtttttacgt
    19441 ttttcataat agtggcctta tgcagatgaa tgacgtcgca agaaattact taacgatcga
    19501 taactaa
//
LOCUS       DAZOZX010000074        19324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZOZX010000074 DAZOZX010000000
VERSION     DAZOZX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.074"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUPT_002523"
     CDS             <1..122
                     /locus_tag="ACHUPT_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0820061.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHUPT_002524"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHUPT_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0820062.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHUPT_002525"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHUPT_002525"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0820063.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUPT_002526"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUPT_002526"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0820064.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUPT_002527"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUPT_002527"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0820065.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUPT_002528"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUPT_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0820066.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUPT_002529"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUPT_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0820067.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHUPT_002530"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHUPT_002530"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR0820068.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKVLGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHUPT_002531"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHUPT_002531"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR0820069.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHUPT_002532"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHUPT_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR0820070.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHUPT_002533"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHUPT_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR0820071.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHUPT_002534"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHUPT_002534"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR0820072.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHUPT_002535"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHUPT_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR0820073.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHUPT_002536"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHUPT_002536"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR0820074.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHUPT_002537"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHUPT_002537"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0820075.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHUPT_002538"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHUPT_002538"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR0820076.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHUPT_002539"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHUPT_002539"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0820077.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..>19324
                     /gene="rfbC"
                     /locus_tag="ACHUPT_002540"
     CDS             18883..>19324
                     /gene="rfbC"
                     /locus_tag="ACHUPT_002540"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR0820078.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGIL"
BASE COUNT         4803 a         4680 c         5269 g         4572 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagtcc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttat
//
LOCUS       DAZOZX010000075        18501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZOZX010000075 DAZOZX010000000
VERSION     DAZOZX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.075"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHUPT_002541"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHUPT_002541"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0820079.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHUPT_002542"
     CDS             complement(1672..2667)
                     /locus_tag="ACHUPT_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0820080.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHUPT_002543"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHUPT_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUPT_002544"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUPT_002544"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0820081.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUPT_002545"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUPT_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0820082.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUPT_002546"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUPT_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0820083.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUPT_002547"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUPT_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0820084.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUPT_002548"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUPT_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0820085.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUPT_002549"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUPT_002549"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0820086.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUPT_002550"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUPT_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0820087.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUPT_002551"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUPT_002551"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0820088.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUPT_002552"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUPT_002552"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0820089.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHUPT_002553"
     CDS             complement(17699..18322)
                     /locus_tag="ACHUPT_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0820090.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         5313 a         4228 c         3973 g         4987 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat t
//
LOCUS       DAZOZX010000076        17971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZOZX010000076 DAZOZX010000000
VERSION     DAZOZX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.076"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..1015
                     /locus_tag="ACHUPT_002554"
                     /pseudo
     CDS             1..1015
                     /locus_tag="ACHUPT_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1161..2198)
                     /gene="iap"
                     /locus_tag="ACHUPT_002555"
     CDS             complement(1161..2198)
                     /gene="iap"
                     /locus_tag="ACHUPT_002555"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0820091.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2450..3358
                     /gene="cysD"
                     /locus_tag="ACHUPT_002556"
     CDS             2450..3358
                     /gene="cysD"
                     /locus_tag="ACHUPT_002556"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0820092.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3360..4787
                     /gene="cysN"
                     /locus_tag="ACHUPT_002557"
     CDS             3360..4787
                     /gene="cysN"
                     /locus_tag="ACHUPT_002557"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0820093.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4787..5392
                     /gene="cysC"
                     /locus_tag="ACHUPT_002558"
     CDS             4787..5392
                     /gene="cysC"
                     /locus_tag="ACHUPT_002558"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0820094.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5442..5765
                     /locus_tag="ACHUPT_002559"
     CDS             5442..5765
                     /locus_tag="ACHUPT_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0820095.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            5959..6270
                     /gene="ftsB"
                     /locus_tag="ACHUPT_002560"
     CDS             5959..6270
                     /gene="ftsB"
                     /locus_tag="ACHUPT_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0820096.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6289..6999
                     /gene="ispD"
                     /locus_tag="ACHUPT_002561"
     CDS             6289..6999
                     /gene="ispD"
                     /locus_tag="ACHUPT_002561"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0820097.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            6999..7478
                     /gene="ispF"
                     /locus_tag="ACHUPT_002562"
     CDS             6999..7478
                     /gene="ispF"
                     /locus_tag="ACHUPT_002562"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0820098.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7475..8524
                     /gene="truD"
                     /locus_tag="ACHUPT_002563"
     CDS             7475..8524
                     /gene="truD"
                     /locus_tag="ACHUPT_002563"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0820099.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8505..9266
                     /gene="surE"
                     /locus_tag="ACHUPT_002564"
     CDS             8505..9266
                     /gene="surE"
                     /locus_tag="ACHUPT_002564"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0820100.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9260..9886
                     /gene="pcm"
                     /locus_tag="ACHUPT_002565"
     CDS             9260..9886
                     /gene="pcm"
                     /locus_tag="ACHUPT_002565"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0820101.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10071..11165
                     /gene="nlpD"
                     /locus_tag="ACHUPT_002566"
     CDS             10071..11165
                     /gene="nlpD"
                     /locus_tag="ACHUPT_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0820102.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11228..12220
                     /gene="rpoS"
                     /locus_tag="ACHUPT_002567"
     CDS             11228..12220
                     /gene="rpoS"
                     /locus_tag="ACHUPT_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0820103.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(12340..12747)
                     /locus_tag="ACHUPT_002568"
     CDS             complement(12340..12747)
                     /locus_tag="ACHUPT_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR0820104.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            12894..13487
                     /locus_tag="ACHUPT_002569"
     CDS             12894..13487
                     /locus_tag="ACHUPT_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR0820105.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13487..14434
                     /locus_tag="ACHUPT_002570"
                     /pseudo
     CDS             13487..14434
                     /locus_tag="ACHUPT_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14707..15270)
                     /locus_tag="ACHUPT_002571"
     CDS             complement(14707..15270)
                     /locus_tag="ACHUPT_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820106.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15435..15686
                     /locus_tag="ACHUPT_002572"
     CDS             15435..15686
                     /locus_tag="ACHUPT_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0820107.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15688..17784
                     /locus_tag="ACHUPT_002573"
     CDS             15688..17784
                     /locus_tag="ACHUPT_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0820108.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4431 a         4396 c         4825 g         4319 t
ORIGIN      
        1 aaaaaccgac gtggcgagaa agagggtatc gacgactatc accttctttg ctggcatttg
       61 ctggatgtgg ccgcaatggg ctatttaatg gttaaaagaa attgcttcgg gctggctgat
      121 tattttcgtc aattagggat ttctgacaag gaacaggcag ctcgattttt cgcctggttg
      181 ctgtgctggc acgatattgg aaaatttgcc cgctcttttc aacaacttta cctgccccct
      241 gaattcaaga ttcaggaagg tgccagaaaa aatctctcat tcaacgctgg gctactggtt
      301 gtggaatcat tatttaagtg aatgtcagga gttacttcct tcatcttcgc tctctccgcg
      361 caaacttagg cgagtgatag aaatgtggat gcccgtgacg acagggcatc atggtcggcc
      421 ccctgatcgt atggatgaat tggataattt tctgcctgaa gacaaagctg cagcacgaga
      481 ttttctcctt gaaatcaagc cactgtttcc gcgaatagag atccccacat tctgggatga
      541 tgacgagggc gttgaactta taaaacaact ttcctggtat atctctgcaa ccgtcgtact
      601 tgcagactgg acagggtcgt caacgcgatt ttttccacgc gtcgcacagg caatggatat
      661 tgaagattac tggcaaaagg ctttagttca ggctcaaaac gcattaaccg tctttccgcc
      721 acaagcagaa accgcgcctt tcaccggaat taatacgctg tttcctttta ttgagaatac
      781 gacaccgtta cagcaaaagg tgctggatct ggatatcaat cagccaggac cacagttgtt
      841 tattctggaa gatgtgaccg gcgcaggtaa aacagaagcg gcgcttatcc tggcgcacag
      901 gttgatggct gcgggtaacg ctcaggggct gttttttggc ctgccaacaa tggcaacggc
      961 caatgccatg tacgatcgac tggtcaaaac ctggcttgct ttctattcgc cagaggtgtt
     1021 ccccgctcca gcggagataa accggcgcaa tacggtgcgg ttttcccgga agtaaaccgg
     1081 acgcactgga tgcgatgatg ggtatcactt agaattcccc gcccctgcgg tagaactccc
     1141 agctcccatt ttcaaaccca tcaagacgcc ttcgccagct ccttcaccaa cggtagcatt
     1201 atccgcataa cgtcacggca gcgacgttct attcttccag gaagtgcctt atcaatatgt
     1261 tgttgattat ccagtcttac gtcatgccag ctatttcccg ccgggaaggc aggtgttttt
     1321 gcgcgttgct gataaccatc cttatttcca agattccagt tagtcgcttc caccgaaagt
     1381 acagcaatgc ccgctttgtc gaatatttct gcgtcattac aacccccagt gcctttcgga
     1441 taatttttat tcaaacccgg attggtcgtt gcggctattc cgtgactgcg tgcaattgcc
     1501 agcgccctgt cgcgcgttaa tttccttact gcttcagggg tttttacacc gctgttgaaa
     1561 tacaatttat cgccaacaat taagttatcg agattaatca ccagcagcgt atttttcttt
     1621 tcggtgtcac tcatccgctt gagtaaattc tcagcgccta attttccttc ctcttcgccg
     1681 ctggtcgcca caaatcgaat accatactcg gtaggcgtat ttttcaggcg ttctgccagt
     1741 tccagcatga cacctaaacc tgcggcgtta tcatctattc cttgtaacgt cagtccgccg
     1801 agattggcat ctgcgtcagc atcgctcagc ggggcgtaag tatccagatg cgccataatg
     1861 atgatctgct gcggcgcttt gccttcatga gcggcaatca ccgtacttcc tgtcacgtta
     1921 tgccagttct tgcgattatc gcgggcggta taaatatacc gactattaaa tgtccgaata
     1981 tcactgcgat aacccatttg ctgaaactgt tggcgaatat aatcggcaga taacatttct
     2041 gctggagttc cggtcatgcg tcccggaaag aaagtagcaa tatgtcgtgc ctgggtatta
     2101 gcaaaatcgc caggtttagg tgacgaggcg tgtacgggga gaataaagca tacgccgagc
     2161 gccagggcag cggtacggtg gcgcaatgcg gaaaacatag tgagtcctta aataccatgc
     2221 aaattttttt accgccatag tatgaaactg ccgctgcgct aaaacaattt caaatcttcc
     2281 taaacgcccg aaatccggtg ccttaagcac tttttgatat tagctttgcc aaatcgttat
     2341 tccgttaagg aactactcat tctaattggt aatttcattc gttctcttac gctccctata
     2401 gtcgaaacat ctgatggcaa gaaaatagcg gtattgcaaa ggaacggtta tggctcaaaa
     2461 acgacttact cacctgcggc aactggaggc ggaaagcatc cacattattc gcgaggtggc
     2521 ggcagaattc tcaaatccgg tgatgctcta ctctatcggt aaagattcca gcgtcatgct
     2581 gcatctggcg cgcaaggcgt tttatccagg tacgctgcct ttcccgttgc tgcatgtcga
     2641 taccggctgg aaattccgcg agatgtatga gttccgcgat cgtacggcga aagcctacgg
     2701 ctgcgagctg ctggtgcata aaaacccgga aggcgtggcg atggggatta atccattcgt
     2761 gcacggcagc gcgaaacata ccgatattat gaaaactgaa ggcctgaaac aggcgctgaa
     2821 caaatacggt tttgatgccg ccttcggtgg tgcgcgccgt gacgaagaga aatcccgcgc
     2881 taaagagcga atctactctt tccgtgaccg cttccatcgc tgggatccga aaaatcagcg
     2941 cccggagctg tggcacaact acaacgggca aattaacaaa ggcgaaagca tccgcgtctt
     3001 cccgctctct aactggaccg agcaggatat ctggcaatac atctggctgg aaaatatcga
     3061 cattgtgccg ctgtatctcg ctgcggaacg tccggttctg gaacgcgacg gtatgttgat
     3121 gatgattgat gacaaccgta tcgacctgca accgggcgaa gtgatcaaaa aacggatggt
     3181 gcgtttccgt acgctgggct gctggccgct gaccggtgcg gtggagtcaa atgcacaaac
     3241 actgccggaa atcatcgaag agatgctggt ttccaccacc agtgaacgtc agggtcgcgt
     3301 gattgaccgc gaccaggcgg gatccatgga gctgaaaaaa cgtcaggggt atttttaaga
     3361 tgaacaccgt acttgcacaa caaatcgcca atgaaggcgg cgtcgaagcc tggatgattg
     3421 cgcaacaaca taaaagcctg ctgcgttttc tgacctgtgg tagcgtcgat gacggcaaaa
     3481 gtaccctgat tggtcgtctg ctgcacgata cccgccaaat ctacgaagat cagctctcat
     3541 cgctgcataa cgacagtaag cgtcacggaa cccagggcga aaagctggat ctggctctgc
     3601 tggtggacgg cctgcaagct gagcgcgaac agggcatcac tattgatgtg gcctaccgct
     3661 atttctctac cgagaagcgt aaatttatta tcgccgacac cccagggcac gagcagtaca
     3721 ctcgcaatat ggcgactggc gcatcgacat gtgaactggc gatcttactg attgatgccc
     3781 gtaaaggcgt gctcgatcaa acccgtcgtc acagttttat ctccacactg ttggggatca
     3841 aacatctggt cgtggcgatc aacaaaatgg atctggtgga ttacagtgaa gagacgttca
     3901 cccgtattcg tgaagattat ctgacctttg ccgggcagct gccgggtaat ctggatatcc
     3961 gctttgtgcc actctccgca ctggaaggtg acaacgtggc ttcgcaaagt gaaagtatgc
     4021 cgtggtacag cggtctgaca ctgctcgaag tgctggaaac cgtggagatc cagcgagtgg
     4081 tggatgccca gccaatgcgc ttcccggtgc agtacgttaa ccgcccaaat ctcgattttc
     4141 gtggctacgc cggaatgctg gcatccggtc gcgtggaagt cgggcaacgt gtaaaagtgc
     4201 tgccctctgg tgtggaatca aacgtcgcgc ggatcgtgac ttttgatggt gatcgcgaag
     4261 aagtctttgc cggagaagcg atcaccctgg tgctgacgga tgagatcgat atcagccgtg
     4321 gtgatctgct gctggcggca gacgaagcgt taccggcggt gcagagtgcg tcggtggatg
     4381 tggtatggat ggcggaacag ccgctttccc cgggccagag ttatgacatc aaaattgccg
     4441 gtaagaagac gcgtgctcgt gttgatggca ttcgttatca ggttgatatt aataacctta
     4501 cccaacgcga agttgaaaac ctgccgctga acggcatcgg cctggtggat ctcacttttg
     4561 acgagccact ggtgttagat cgctatcagc aaaatccggt taccggtggg ctgattttta
     4621 tcgatcgcct gagcaatgtg accgtgggtg ccggtatggt gcacgagcca gttagccagg
     4681 caacagctgc gtcatctgaa ttcagtgcat tcgaactgga attgaatgcc ctggttcgtc
     4741 gccactttcc gcactggggc gcgcgcgatt tgctggggga taaataatgg cgctgcatga
     4801 cgaaaacgtc gtctggcata gtcatccggt cactgtgcaa caacgcgaac tacaccacgg
     4861 tcatcgtggt gtagtgctgt ggtttaccgg cctctccggg tccggtaaat caacggtcgc
     4921 cggggcgctg gaggaggcgt tacataaact cagcgtcagt acgtatctgc tggatggcga
     4981 caatgttcgc cacggattat gcagcgatct cggttttagc gatgccgatc gtaaagagaa
     5041 tatccgtcgc gtcggtgaag tggcgaattt gatggttgaa gccggactgg tggtgctgac
     5101 cgcatttatc tcgccacacc gcgccgaacg ccagatggtt cgcgaacgcg taggagaagg
     5161 gcgctttatt gaagtgtttg tcgatacgcc gctggcgatt tgcgaagccc gcgatccaaa
     5221 aggcttatat aagaaagcgc gtgccggtga actgcgcaac tttacgggaa tagattccgt
     5281 ttacgaagcg cctgaatcgg cagaaattca tctcaatggt gaacaattag taacaaattt
     5341 ggtacagcaa ttattagatc tgttgagaca gaacgatatt atcagatcct gagacaccac
     5401 cgggtttcca tgcccggttc gtcaaagtaa caggatcaaa tatgcgtaat agccataaca
     5461 ttacactaac aaataacgac agccttactg aggatgaaga aactacatgg tcactgcctg
     5521 gtgccgtggt cggttttatc tcctggttat ttgcgctggc gatgccgatg ttgatttatg
     5581 gctctaacac gctgttcttc tttctctaca cctggccttt ctttctggca ctgatgcctg
     5641 tcgcggtagt ggtggggatt gcgctgcatt cattgatgga cggaaagcta cgctacagta
     5701 ttgttttcac cctggtgaca gtaggcatta tgtttggcgc actgtttatg tggctactgg
     5761 gctaatttgt actttcttgt ctcctctgtt cataattcaa accgtaacta ataatgagat
     5821 tatgttctgc acgccctggg tatacgtaac aatgggcaaa tgtggtacat ttgcccgcgt
     5881 tgtcgcggta tccccaacag aggatgtaga gtcgtcttcg gatgcatggg atgatgatgc
     5941 agtttttcag ggggcaggat gggtaaacta acgctgctgt tgctggctat tctggtctgg
     6001 ctacagtatt cgctgtggtt cggtaagaac ggtatacatg actatacccg cgtcaatgat
     6061 gatgtggcgg cactgcaagc tacaaacgcg aaacttaaag cgcgaaacga tcaacttttt
     6121 gccgaaattg acgatctcaa tggcggccag gaggcgctcg aagagcgtgc gcgtaatgaa
     6181 ctcagcatga ccaggccggg cgaaactttt tatcgtctgg tgcctgacgc gtcgaagcgc
     6241 gcacagtctg cggggcaaaa caatcgataa atcagcccgg gaattaacat ggcaaccact
     6301 catttggatg tttgcgccgt ggttccggcg gccggatttg gccgtcgaat gcaaacggaa
     6361 tgtcctaagc aatatctctc aatcggtaat caaaccattc ttgaacactc ggtgcatgcg
     6421 ctgctggcgc atccccgggt gacacgtgtc gtcattgcca taagtcctgg cgatagccgt
     6481 tttgcacaac ttcctctggc gaatcatccg caaatcaccg ttgtagatgg cggtgatgag
     6541 cgtgccgatt ccgtgctggc aggtctgaaa gccgctggcg atgtgcagtg ggtattggtg
     6601 catgacgccg ctcgtccttg tttgcatcag gatgaccttg cgcgattgtt ggcgttgagc
     6661 gaaaccagcc gcacgggagg gatcctcgcc gcaccagtgc gcgatactat gaaacgtgcc
     6721 gaaccgggca aaaatgccat tgctcatacc gttgatcgca acggcttatg gcacgcgctg
     6781 acgccgcaat ttttccctcg tgagctgtta catgactgtc tgacgcgcgc tctaaatgaa
     6841 ggcgctacca ttaccgacga agcctcggcg ctggaatatt gcggatttca tccgcagttg
     6901 gtcgaaggcc gcgcggataa cattaaagtc acgcgcccgg aagatttggc actggccgag
     6961 ttttacctca cccgaaccat ccatcaggag aatacataat gcgaattgga cacggttttg
     7021 acgtacacgc ctttggcggt gaaggcccaa ttatcattgg tggcgtacgc attccttacg
     7081 aaaaaggatt gctggcgcat tctgatggcg acgtggcgct tcatgcgttg accgatgcat
     7141 tgcttggcgc ggcggcgctg ggggatatcg gcaaactgtt cccggatacc gatccggcat
     7201 ttaaaggtgc cgacagccgc gagctgctac gcgaagcctg gcgtcgtatt caggcgaagg
     7261 gttataccct tggcaacgtc gatgtcacta tcatcgctca ggcaccgaag atgttgccgc
     7321 acattccaca aatgcgcgtg tttattgccg aagatctcgg ctgccatatg gatgatgtta
     7381 acgtgaaagc cactactacg gaaaaacttg gatttaccgg acgtggggaa gggattgcct
     7441 gtgaagcggt ggcgctactc attaaggcaa caaaatgatt gagtttgata atctcactta
     7501 cctccacggt aaaccgcaag gcaccgggct gctgaaagcc aatccggaag actttgtggt
     7561 ggtggaagat ttgggctttg agcctgatgg tgaaggtgag catattctgg ttagaatcct
     7621 caaaaacggc tgcaataccc gttttgtggc ggatgcactg gcgaaattcc tgaaaattca
     7681 ttcccgtgaa gtcagcttcg ctgggcaaaa agacaaacat gctgtaacgg aacagtggtt
     7741 atgtgctcgc gtgccgggca aggaaatgcc cgatctgagc gcctttcaac tggaaggctg
     7801 ccaggtgctg gagtatgcgc ggcacaagcg caagctgcgt ttaggggcgc tgaaaggtaa
     7861 cgcctttacc ctggttctgc gcgaagtgag caatcgcgat gacgttgaac aacgtctgat
     7921 cgatatttgc gtaaaaggtg taccgaacta cttcggtgcc caacgttttg ggattggcgg
     7981 tagcaacttg cagggcgcgc tgcgctgggc gcaaaccaat actccggtgc gcgatcgcaa
     8041 taaacggagt ttttggttgt cggcagcccg cagtgcgttg tttaatcaga ttgttgctga
     8101 gcgcctcaaa aaagcagacg ttaatcaagt tgttgacggc gatgcgctac aattagccgg
     8161 acgtggtagc tggtttgtag caaccaccga agaactggcg gaattacagc gtcgcgtcaa
     8221 cgataaagag ctgatgataa ccgccgcatt gccgggcagt ggcgaatggg gaactcagcg
     8281 tgaagcgctg gcattcgaac aagcagctgt cgccgcagaa actgaattac aagctttact
     8341 ggtgcgcgaa aaagttgaag ccgcgcgcag agcgatgctg ctgtatccgc aacaattaag
     8401 ctggaactgg tgggatgacg tcaccgtaga gatccgtttc tggcttccgg cgggtagttt
     8461 tgcaaccagc gttgtcaggg aacttatcaa cacaacaggt gattatgcgc atattgctga
     8521 gtaattatga cggggtacat gcacccggta tacaaacgct ggcgaaagcc ttgcgtgagt
     8581 ttgctgacgt tcaggtggtc gcccccgatc gtaaccgcag cggcgcttca aattctctga
     8641 cactggaatc ctccctgcgc acgtttacct ttgaaaatgg tgatattgct gtgcaaatgg
     8701 gaaccccgac cgattgtgtc tatcttggcg tgaatgctct gatgcgtccg cgcccggaca
     8761 ttgttgtgtc cggaattaac gccgggccga atctggggga tgatgttatt tattccggta
     8821 cggtagccgc cgcgatggaa ggccgtcatt taggttttcc ggcgctggcc gtctcgcttg
     8881 acgggcataa acattacgac actgccgcgg cggtaacctg ttcaattttg cgcgcactgt
     8941 gtaaagagcc gctgcgcacc ggacgtattc ttaatattaa cgttcaggat ttacccttgg
     9001 atcaaatcaa aggtattcgc gtgacgcgct gcggtacacg acatccggca gatcaggtga
     9061 tcccgcagca agatccgcgc ggcaatacgc tgtactggat tggcccgccg ggcggtaaat
     9121 gtgatgccgg tccggggacc gattttgctg cggtagatga gggctatgtc tccatcacgc
     9181 cgctgcatgt ggatttaact gcgcatagcg cgcaagatgt ggtttcagac tggttaaaca
     9241 gcgtgggagt tggcacgcaa tggtaagcag acgcgtacaa gcacttctgg atcaattacg
     9301 tgcgcaaggt attcaggatg agcaggtgct gaatgcactt gccgccgtgc cgcgtgaaaa
     9361 attcgttgat gaagcgtttg aacaaaaagc ctgggacaat atcgccttgc cgataggtca
     9421 ggggcagaca atttcgcagc cgtatatggt ggcgcgaatg accgaattac tcgagctgac
     9481 gccgcagtcg cgggtgctgg aaattggcac tggttcggga tatcaaacgg caatcctggc
     9541 gcatcttgtc cagcatgttt gctcggttga acggattaaa ggcttgcagt ggcaggcacg
     9601 tcgccgcctg aaaaatcttg atttacataa tgtttcaacc cgtcatggtg atggatggca
     9661 aggttggcag gcacgtgcgc cgtttgacgc tatcattgtt acggcggcac cgccggaaat
     9721 tccaactgcg ctaatgacgc agctggacga aggcgggatt ctcgtcttac ccgtagggga
     9781 ggagcaccag tatttgaaac gggtgcgtcg tcggggaggc gaatttatta tcgataccgt
     9841 ggaggccgtg cgctttgtcc ctttagtgaa gggtgagctg gcttaaaacg tgaggaaata
     9901 cctggatttt tcctggttat tttgccgtag gtcagcgtat cgtgaacatc ttttccagtg
     9961 ttcagtaggg tgccttgcac ggtaattatg tcactggtta ttaaccaatt tttcctgggg
    10021 gataaatgag cgcgggaagc ccaaaattca ccgttcgccg cattgcggct ttgtcactgg
    10081 tttcgctatg gctggcaggc tgttctgaca cttcaaatcc accggcaccg gtcagctccg
    10141 ttaatggcaa tgcgcctgca aatactaatt ctggtatgtt gattacgccg ccgccgaaaa
    10201 tggggacgac gtctacagcg cagcaaccgc aaattcagcc ggtacagcag ccacaaattc
    10261 aggctactca acaaccgcaa atccagccag tgcagccagt agctcagcag ccggtacaga
    10321 tggaaaacgg acgcatcgtc tataaccgtc agtatgggaa cattccgaaa ggcagttata
    10381 gcggcagtac ctataccgtg aaaaaaggcg acacactttt ctatatcgcc tggattactg
    10441 gcaacgattt ccgtgacctt gctcagcgca acaatattca ggcaccatac gcgctgaacg
    10501 ttggtcagac cttgcaggtg ggtaatgctt ccggtacgcc aatcactggc ggaaatgcca
    10561 ttacccaggc cgacgcagca gagcaaggag ttgtgatcaa gcctgcacaa aattccaccg
    10621 ttgctgttgc gtcgcaaccg acaattacgt attctgagtc ttcgggtgaa cagagtgcta
    10681 acaaaatgtt gccgaacaac aagccaactg cgaccacggt cacagcgcct gtaacggtac
    10741 caacagcaag cacaaccgag ccgactgtca gcagtacatc aaccagtacg cctatctcca
    10801 cctggcgctg gccgactgag ggcaaagtga tcgaaacctt tggcgcttct gaggggggca
    10861 acaaggggat tgatatcgca ggcagcaaag gacaggcaat tatcgcgacc gcagatggcc
    10921 gcgttgttta tgctggtaac gcgctgcgcg gctacggtaa tctgattatc atcaaacata
    10981 atgatgatta cctgagtgcc tacgcccata acgacacaat gctggtccgg gaacaacaag
    11041 aagtgaaggc ggggcaaaaa atagcaacca tgggtagcac cggaaccagt tcaacacgct
    11101 tgcattttga aattcgttac aaggggaaat ccgtaaaccc gctgcgttat ttgccgcagc
    11161 gataaatcgg cggaaccagg cttttgcttg aatgttccgt caagggatca cgggtaggag
    11221 ccaccttatg agtcagaata cgctgaaagt tcatgattta aatgaagatg cggaatttga
    11281 tgagaacgga gttgaggttt ttgacgaaaa ggccttagta gaagaggaac ccagtgataa
    11341 cgatttggcc gaagaggaac tgttatcgca gggagccaca cagcgtgtgt tggacgcgac
    11401 tcagctttac cttggtgaga ttggttattc accactgtta acggccgaag aagaagttta
    11461 ttttgcgcgt cgcgcactgc gtggagatgt cgcctctcgc cgccggatga tcgagagtaa
    11521 cttgcgtctg gtggtaaaaa ttgcccgccg ttatggcaat cgtggtctgg cgttgctgga
    11581 ccttatcgaa gagggcaact tggggctgat ccgcgcggta gagaagtttg acccggaacg
    11641 tggtttccgc ttctcaacat acgcaacctg gtggattcgc cagacgattg aacgggcgat
    11701 tatgaaccaa acccgtacta ttcgtttgcc gattcacatc gtaaaggagc tgaacgttta
    11761 cctgcgaacc gcacgtgagt tgtcccataa gctggaccat gaaccaagtg cggaagagat
    11821 cgcagagcaa ctggataagc cagttgatga cgtcagccgt atgcttcgtc ttaacgagcg
    11881 cattacctcg gtagacaccc cgctgggtgg tgattccgaa aaagcgttgc tggacatcct
    11941 ggccgatgaa aaagagaacg gtccggaaga taccacgcaa gatgacgata tgaagcagag
    12001 catcgtcaaa tggctgttcg agctgaacgc caaacagcgt gaagtactgg cacgtcgatt
    12061 cggtttgctg gggtatgaag cggcaacact ggaagatgta ggtcgtgaaa ttggcctcac
    12121 ccgtgaacgt gttcgccaga ttcaggttga aggcctgcgc cgtttgcgcg aaatcctgca
    12181 aacgcagggg ctgaatatcg aagcgctgtt ccgcaagtaa gtaagcatct gtcagaaagg
    12241 ccagtctcaa gcgaggctgg cctttttctt ttgtttggga agcttgtgtt tttgtcgcct
    12301 ggataagaca cgtcagcgtc ctacccggca atcagcattc tatgtgctca tcatcttgcg
    12361 caccaactgc ataaacagct cctgctcttc tccactcaaa cgccccaaaa attcctcatc
    12421 cacgctgtca ccaataggta tcgccgcagc aagtagcttt tctccttcgg cagtcagcca
    12481 gacaaagcgt cgccgcttgt cagcgacgtc atgttcacgt ctgactaagc cacgattttc
    12541 cattcttgcc aacatttctg ctagcgttgc tttggtgctg actgctgctt ctatcagtgc
    12601 cacctgttca ataccaggtt tatcagcaat ggcgcgcatc accgcgtact gtggtttggt
    12661 caggtcaggt aactcgtgct gccagcgagc cgtgtgctgc tgaaaaagct gtcgtaactg
    12721 atggaacgct ttatttcgta acgccatgta aactccgggc taattctctg tcgctatcat
    12781 agcggttttt tagtgacgaa gaagatgttg ttacttttca atccttgccg ctgtggaaat
    12841 gaaggagtat gttaataata acgttcgtat acgaataatt aagagaataa gcaatgaaac
    12901 tgatcgtcgg gatgacaggg gctaccggtg cgcctcttgg tgtggcatta ctgcaagcgc
    12961 tgcgtgagat gccgaatgtc gagactcatc tggtgatgtc gaagtgggcg aaaaccacca
    13021 ttgaactgga aacgccttac agcgctcgcg atgttgctgc cctcgcagac ttcagccata
    13081 acccggcgga tcaggcggcg atcatctcat ccggttcttt tcgtaccgac ggcatgatcg
    13141 ttattccgtg cagtatgaaa acgctcgccg gtatccgcgc tggttacgcc gatggcctgg
    13201 tagggcgcgc ggcggacgtc gtgctcaaag aaggccgcaa actggtgctg gtgccgcgtg
    13261 aaatgccgct tagcaccatc catctcgaaa atatgctcgc actttcacgc atgggcgtgg
    13321 cgatggtgcc gccgatgcct gccttttata accatcccga aacggtagat gacattgtcc
    13381 accatgtggt agcccgcgtg ctggatcaat ttggcctcga acatccccac gccaggcgct
    13441 ggcaaggatt gccgcaggcc cggaattttt ctcaggagaa tgaataatgg catttgatga
    13501 tttacgcagc tttttacagg cgcttgatga ccacggccag ttactgaaaa tcagcgaaga
    13561 agtgaacgct gagccggatc tggcagcagc agctaacgcc accgggcgta tcggcgacgg
    13621 cgcgcccgcg ctgtggtttg ataatattcg cggctttacc gatgcccgcg tggcgatgaa
    13681 taccatcggt tcctggcaga accacgcgat ttcccttggc ctgccaccaa acaccccggt
    13741 caaaaagcag attgatgagt ttatccgccg ctgggataac ttcccgattg ccccggagcg
    13801 ccgcgccaat ccagcctggg cgcagaacac cgtcgatggc gaagagatca acctgttcga
    13861 tattctgccg ctgtttcgcc tgaatgatgg cgatggcggt ttctatctcg acaaagcgtg
    13921 cgtggtttcc cgcgatccgc tcgacccgga taacttcggc aagcagaacg tcggcatcta
    13981 ccgcatggaa gtgaagggca agcgtaagct cggcctgcaa ccggtgccga tgcacgatat
    14041 cgccctgcat ctgcataaag cagaagagcg cggtgaagat ctgccgattg cgatcacgct
    14101 cggtaacgat ccgatcatca cgctgatggg ggccacgccg ctgaaatacg atcagtctga
    14161 gtatgaaatg gcaggcgcgc tgcgtgaaag cccgtacccg atcgccaccg ctccgctgac
    14221 cggttttgat gtgccgtggg gttcagaagt gatcctcgaa ggggttatcg aaagccgtaa
    14281 acgcgaaatc gaagggccgt tcggtgagtt taccgggcac tactccggtg ggcgcaacat
    14341 gaccgtggtg cgcatcgata aagtctctta tcgcagcaaa ccgattttcg aatcactcta
    14401 tctcggtatg ccgtggaccg aaatcgacta cctatgacta ggtaatttat tcaagataac
    14461 cgggaagaga ttaaaaaaga cgggaataga ttggaatcaa caagttagga actcaaggtt
    14521 ttcaagataa agcgagtcca aaaatgttaa acggttcgat atcaataaaa cattcaagat
    14581 aactcacagg ttcttacaat agcctttaaa gaccgttcaa aaacgattca ggtgtttatt
    14641 atagcgctga tgggtgcaag cgccaaaacc aatgtaattt tagcacagct aggcacttgc
    14701 actgggttat gcgctggctg ctttctgagg tagatttaag tgatcgccct tagcttcgga
    14761 caacgcgtaa atctctctaa ccttttcgtc tggcaaatta ccaaggctta acgctatggc
    14821 tctacgaact ccaactagag cgtgcaactc ttggatgtgc tcatccagaa gaatgagtaa
    14881 ttgctccgct ccttttctcc caagaacatc agctacttgt tgtttttctt gtggtttaag
    14941 aaatgacaag aacattagaa tttgagcatc aacattatcc gcattctctt ttttgggttc
    15001 tttggaatct tttagttcaa catcaccctc tcccgtcatg agccattcca aggtaacatt
    15061 ttcgctttct gctatctttt ttaactgata cgggcgtggt atcggtgcgt aatcccgcct
    15121 gttgtaataa ttttttaaag tgccttcatt gatcccccat gcccgagcag ccgcatttct
    15181 tgaagggtat cgttctacta acctaaagat tctttcgata atcggttctt ttccatcggc
    15241 aggaaaagaa ccttggggtg cttttctcat tcttggttcc tttctatcta attcattgat
    15301 aaataataaa attaaaaaat aaaccataaa aacaccaaag aaccctttgc agggaatctt
    15361 tggtatctta atctttatcc atacggatat ccgaccggat atccgcgcgg gtaattttgt
    15421 aggttaaagg aaagatgaga caaagagaag atagtgaccg cgattggcct tcgctgaaga
    15481 ttcagctggc gctcaaagat aacgggtggt cttccttccg ggagctttca ctccatgctg
    15541 gttatgctca ggattcactg aaagcggtag ttcgcacacc aaacaaaaaa tacgaacgcg
    15601 taatttctga cgctataggt gtaccgccag aaatcatctg gcccagccgg tatcgtaacg
    15661 atggatttat caggaaggcg tcgtgatatg cagtcgtacg ttaccgtaaa tgacctgctc
    15721 ggcgtaccag gaatgccagc caccacaaaa ggcatccgac aggcgttaca gcgtttttct
    15781 cgtgatcttg gtgacgttag tcgccgtcgg gaaggcacaa aagccattga gtaccacatt
    15841 gattgtctgc ctgaaataac acgaaaggca ctgcgtgaac gctatgtgga acagctggtg
    15901 gctacagaga acaatgtttc tgaagtgaaa gctgttacca gaaaaacacg caatcctgac
    15961 gctgtccagg caatcgaagc atatcgcggt tctccacaac tgatggaaga acgcctgaat
    16021 gcgctgaccg aaaaccagcg ctgggtatct gaagcaagag ctgcgctggt ggtggaagtg
    16081 ctgaagctgg aaagcgccgg taaccccggg cgactgaaag ccattaactt tcttgttgaa
    16141 aaagcccgta aaggtgagct gccggagcgc ctgcaacagg ccgcagttaa cgccaatgca
    16201 aaacgtggcg ctaatcgtac tatcagccgc gatccacttt accagtgggt tctgaaatac
    16261 aaccagtcgc agaacgccgc cgaacggctg ctgttactgg caccgggaaa acgcgacgaa
    16321 ataaaaccgg aagaaatcag ctggctgcct gaatttctgg cgcagtatcg ccaggtgaac
    16381 ggcagaccca tgtcagaagc ctacgaggat tttgtcgcag aatggcagcg tcgccatgcg
    16441 gacgaaccgt acatgctgga ggtgatgcct tcctatgatg ttgtccggta tgccatgaaa
    16501 aaactgccgg aagtggtgaa acaaaaaggg cgcgttaccg gcagtgaata ccgtcagctt
    16561 gaaggattca cccgccgcga ctggacagct atgccggtga attatgtctg gattggtgac
    16621 ggtcacggta tgaagctgaa atgtgcgcat ccgatccacg ggcgtccatt ctcaccggaa
    16681 gtcacgtttg tgatcgatgg tggaacacgg tttgttgttg gctggagtct tgaccttgcc
    16741 gaaaacgtgt tcgccgttgc cggtgcgata cagcacggca ttcgcaacca cggaaaaccg
    16801 ttcctgtatt actcggataa cggttccggt gaaacggctg acatgctgga caaagaggtc
    16861 gtgggtattc tgccacgcct ggggattaaa caccctaccg gtattgccgg taatccacag
    16921 ggacgcggca ttattgaacg cctgaaccgg acactgccga tgcgtatagc gaggaggtat
    16981 cgcacctatt ttggcaaggg tgctgaccgg gaatcgttgc gtgtacttaa ccgtgatctg
    17041 cgttcagcgt ttaacgccct gcaacaggac aagcctctga acgaccgcca gaaagcggca
    17101 atgcgcgagc tgccatcgtg ggcggagctt attgaggcta ttcgtgaagg cgtggaatgg
    17161 tacaacaacc gcccgcattc tgagctgccg atgaaaccga acggacggca ttacagcccg
    17221 acagagttca gaaaaaaacg ccaggcagaa gaagacaccg aaattgagtg gctgagtgat
    17281 cttgagctgc gcgacatgtt ccgcccaatg gtggaacgac cggtaagacg ctgcgaaatt
    17341 cagtggctga acaatattta ctacgcgccg gagttgcgtg acgaacacgg tcgcaaagtg
    17401 cttatcagtt atgacattca tgacgccgag cgaattacgg tacgtcgcaa ggacggcagc
    17461 tttatctgtg aggcgatatg gaacggcaat aaacgcgcag cctttgctgt cagtgctgaa
    17521 tatcacaaac aacagcagcg tattaaagga atgcgcaaac gcgctgagga aaaaattcgt
    17581 gatgctgaag atgaaggtat tcagattctg gaacacaaac aggctgaacc ctggctgagt
    17641 aatgtttatc gcccggttgg taatgtggtg gcagttcagc aaccggaata tgaagaagag
    17701 catgacgaag aatttgaacg cgatttccgg ctggggatgc aaaaactttt tgccatgcag
    17761 gaagaagatg atccgctggc ctgaataaaa aaacagtccg cgggaacgga ctgttaaata
    17821 aaggaaacaa agctgatttg aaataaccga ggaaatacta tgactgatat tgatgatgtt
    17881 tgaggtgtac tggcaatagc ggacactacc atttgttctt tttttaagca gccatctgat
    17941 gatatttttc cctgaaggct gccggggaga t
//
LOCUS       DAZOZX010000077        17796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZOZX010000077 DAZOZX010000000
VERSION     DAZOZX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.077"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..508
                     /locus_tag="ACHUPT_002574"
     CDS             <1..508
                     /locus_tag="ACHUPT_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR0820109.1"
                     /translation="AAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSV
                     SINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDK
                     AIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSS
                     LVMRVAQD"
     gene            589..1263
                     /locus_tag="ACHUPT_002575"
     CDS             589..1263
                     /locus_tag="ACHUPT_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0820110.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1279..3741
                     /locus_tag="ACHUPT_002576"
     CDS             1279..3741
                     /locus_tag="ACHUPT_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0820111.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3757..4790
                     /locus_tag="ACHUPT_002577"
                     /pseudo
     CDS             3757..4790
                     /locus_tag="ACHUPT_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4872..5210)
                     /gene="rcnB"
                     /locus_tag="ACHUPT_002578"
     CDS             complement(4872..5210)
                     /gene="rcnB"
                     /locus_tag="ACHUPT_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0820112.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5429..6259)
                     /gene="rcnA"
                     /locus_tag="ACHUPT_002579"
                     /pseudo
     CDS             complement(5429..6259)
                     /gene="rcnA"
                     /locus_tag="ACHUPT_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6381..6653
                     /gene="rcnR"
                     /locus_tag="ACHUPT_002580"
     CDS             6381..6653
                     /gene="rcnR"
                     /locus_tag="ACHUPT_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR0820113.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6745..6841
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6876..7664
                     /gene="thiM"
                     /locus_tag="ACHUPT_002581"
     CDS             6876..7664
                     /gene="thiM"
                     /locus_tag="ACHUPT_002581"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0820114.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7661..8461
                     /gene="thiD"
                     /locus_tag="ACHUPT_002582"
     CDS             7661..8461
                     /gene="thiD"
                     /locus_tag="ACHUPT_002582"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0820115.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8526..9343
                     /locus_tag="ACHUPT_002583"
                     /pseudo
     CDS             8526..9343
                     /locus_tag="ACHUPT_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9395..10141
                     /locus_tag="ACHUPT_002584"
     CDS             9395..10141
                     /locus_tag="ACHUPT_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0820116.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10115..11080)
                     /locus_tag="ACHUPT_002585"
     CDS             complement(10115..11080)
                     /locus_tag="ACHUPT_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0820117.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11077..12080)
                     /locus_tag="ACHUPT_002586"
                     /pseudo
     CDS             complement(11077..12080)
                     /locus_tag="ACHUPT_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12120..13172
                     /gene="fbaB"
                     /locus_tag="ACHUPT_002587"
     CDS             12120..13172
                     /gene="fbaB"
                     /locus_tag="ACHUPT_002587"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0820118.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13480..14334
                     /gene="gatY"
                     /locus_tag="ACHUPT_002588"
     CDS             13480..14334
                     /gene="gatY"
                     /locus_tag="ACHUPT_002588"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0820119.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14363..15625
                     /gene="gatZ"
                     /locus_tag="ACHUPT_002589"
     CDS             14363..15625
                     /gene="gatZ"
                     /locus_tag="ACHUPT_002589"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR0820120.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15635..16087
                     /gene="gatA"
                     /locus_tag="ACHUPT_002590"
     CDS             15635..16087
                     /gene="gatA"
                     /locus_tag="ACHUPT_002590"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0820121.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16118..16402
                     /gene="gatB"
                     /locus_tag="ACHUPT_002591"
     CDS             16118..16402
                     /gene="gatB"
                     /locus_tag="ACHUPT_002591"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0820122.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16406..17761
                     /locus_tag="ACHUPT_002592"
     CDS             16406..17761
                     /locus_tag="ACHUPT_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0820123.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4625 a         4083 c         4541 g         4547 t
ORIGIN      
        1 tgctgccgct gtcttttctt ctttttttat gagcgctgga gtatttgctg cagacgttga
       61 taccggaaca ttaaccatta aaggaaatat tgcagaatct ccgtgtaaat tcgaagcggg
      121 tggtgattca gtaagtatta atatgccgac tgtaccaacc actgtctttg aaggtaaagc
      181 taaatattct acctatgatg atgcagtcgg tgtaaccagc agcatgttaa aaattagctg
      241 cccgaaagaa gttgctggtg taaaactctc gttgatcaca aacgacaaaa taaccggtaa
      301 cgataaggcg atagccagta gcaacgatac cgtaggtgat aacagcgatg tcctagatgt
      361 ttctgcacct tttaacattg agagttataa aacagcggaa ggtcaatatg ctattccgtt
      421 taaagcaaaa tacctgaaac tgacagataa ctcagtgcaa tcaggtgatg tgttatcttc
      481 tctggttatg cgtgtggcgc aggattaata aataaaattg caagtttctc caggtaataa
      541 acatggccgc tatccccatg gcggcttttt aatttaagag gcattaagat gaaaggatta
      601 ttatctttac tcattttttc tatggtcctt cctgcacatg ccggaattgt tatctacggg
      661 acgcgcatta tttacccggc agaaaataaa gaagttatgg tgcagttgat gaaccaggga
      721 aatcgttctt cgctgctgca ggcgtggatt gatgatggcg atacgtcgtt accaccagaa
      781 aaaattcagg ttcctttcat gttaacgcca ccagtggcaa aaataggggc aaattccggg
      841 cagcaagtaa aaatcaaaat tatgccgaat aaactgccca ctaataaaga aagcattttt
      901 tatctgaatg ttctgtatat tccaccaaat agtccagagc aagaaggcaa gaatgcactg
      961 aagtttgcga tgcaaaacag aattaagttg ttttaccggc cagcgggtat tgctccggta
     1021 aataaagcga cctttaaaaa gttactggta aatcgcagtg gcaatggatt ggtgataaaa
     1081 aatgactcag ctaattgggt gacgatttcg gatgtcaaag ctaataatgt taaagtcaat
     1141 tatgaaacta ttatgattgc ccccttagaa agtcagagtg ttaatatcaa aagtaataat
     1201 gcaaataact ggtatctgac cattatcaat gaccacggca actatattag tgacaaaatt
     1261 taattgcagg agctgcctat gttgagaatg accccacttg catcagcaat cgtagcgtta
     1321 ttgctcggca ttgaagctta tgcagctgaa gaaacctttg atacccattt tatgataggt
     1381 ggaatgaaag accagcaggt tgcaaatatt cgtcttgatg ataatcaacc cttaccgggg
     1441 cagtatgaca tcgatattta tgtcaataag caatggcgcg ggaaatatga gattattgtt
     1501 aaagacaacc cgcaagaaac atgtttatca agagaagtta tcaagcggtt aggcattaat
     1561 agcgataact tcgccagcgg taagcaatgt ttaacatttg agcaacttgt tcagggtggg
     1621 agctatagct gggatatcgg ggtttttcgt ctcgatttca gtgtcccgca ggcttgggtg
     1681 gaagaactgg aaagtggcta tgttccaccg gaaaactggg agcggggtat taatgcgttt
     1741 tatacttctt attatgtgag tcagtattac agcgactata aagcgtcggg taacaacaag
     1801 agtacatatg tacgttttaa cagcgggtta aatttactgg agtggcaact gcattctgat
     1861 gccagtttca gtaaaacaaa taacaatcca ggggtgtgga aaagcaatac cctgtatctg
     1921 gaacgtggat ttgcccaatt tctcggcacg cttcgcgtgg gtgatatgta cacatcaagc
     1981 gatatttttg attctgttcg cttcagcggt gtgcggttgt ttcgtgatat gcagatgttg
     2041 cctaactcga aacaaaattt tacgccacgg gtgcagggga ttgctcagag taacgcgctg
     2101 gtaactattg aacagaatgg ttttgtggtt tatcagaaag aggttcctcc tggcccgttt
     2161 gcgattacag atttgcagtt ggccggtggt ggagcagatc ttgatgttag cgtgaaagag
     2221 gcggacggct cggtaaccac ctatctggtg ccttatgcag cggtgccaaa tatgctgcaa
     2281 cccggcgtgt cgaaatatga ttttgcggcg ggggcgagca agcaaagtga ttttgtccag
     2341 gcaggttatc agtatggttt taataactta ttgacgctgt atggcggcac gatggttgct
     2401 aataattact atgcgtttac cctcggaacg ggttggaaca cgcgcattgg cgcaatttcc
     2461 gtcgatgcca cgaagtcgca tagtaaacaa gacaacggcg atgtgtttga cgggcaaagt
     2521 tatcaaattg cctacaacaa atttgtgagc caaacgtcga cgcgttttgg tctggcggcc
     2581 tggcgttatt cgtcgcgtga ttaccggaca tttaacgatc acgtttgggc aaacaataaa
     2641 gataattatc gccgtgatga aaacgatatc tatgacattg ccgattatta ccagaacgat
     2701 tttggccgta aaaatagctt ttctgccaat atgagccagt cattgccaga aggctggggt
     2761 tctgtgtcct taagtacgtt atggcgagat tactgggggc gtagcggcag cagtaaggat
     2821 tatcagttga gttattccaa taactggcgg cggataagct ataccctcgc ggcaagccag
     2881 gcgtatggcg agaatcatca tgaagagaaa cgttttaata tttttatatc aattccctgt
     2941 gattggggtg atgacgttac gacgcctcgt cggcaaatat atatgtctaa ctcaacgacg
     3001 tttgatgatc agggttttgc ctcaaataat acgggattat caggaacagt agggagtcgg
     3061 gatcagttca attatggtgt caacctgagt catcaacatc agggaaatga aacgacagct
     3121 ggggcgaatt tgacctggaa cgcgccggtt gcgacagtga atggcagtta tagtcagtcg
     3181 agtacttatc gacagactgg agccagtgtt tcagggggca ttgtcgcctg gtcgggtggc
     3241 gttaatctgg cgaaccgtct ttccgaaacg tttgctgtga tgaatgcgcc aggaattaaa
     3301 gatgcttatg tcaatgggca aaaatatcgc acaacaaacc gtaatggagt ggtggtatac
     3361 gacggaatga caccttatcg ggaaaatcac ctgatgctgg atgtgtcgca aagcgatagc
     3421 gaagcagaat tacgtggcaa ccggaaaatt gccgcccctt atcgcggcgc ggttgtactg
     3481 gttaattttg ataccgatca gcgcaagccc tggtttataa aagcgttaag agcggatggt
     3541 caaccattaa cgtttggtta tgaagtcaat gatatccatg gtcataatat tggcgttgtc
     3601 ggccagggaa gccagttatt tattcgcacc aatgaaatac cgccatcggt taatgtagca
     3661 attgataagc aacaaggact ttcatgcaca atcaccttcg gtaaagagat tgatgaaagt
     3721 agaaattata tttgccagta atagtcaggt ggttctatgg aaattcgcat aatgctattt
     3781 atattaatga tgatggttat gcctgtgagc tatgcggcat gttatagtga gttatctgtt
     3841 cagcacaatt tggttgttca ggggaatttt gcacttactc aaacacaaat agcgacatat
     3901 gagcataatt ttaatgattc gtcatgcgtg agtacaaata ctatcacccc tatgagccca
     3961 tcggatatta ttgttggact ttataacgat accattaaat taaatttaca ttttgaatgg
     4021 accaataaaa acaacatcac gttgtcaaat aatcagagca gtttcaccag tggttattcg
     4081 gtaacggtga cacctgcggc cagtaatgca aaagtgaata tttctgcggg gggcggtggt
     4141 tcagtgatga ttaatggtgt tgcgacatta tccagtgctt catcatcgac acgcgggagt
     4201 gccgcagtac aatttctact gtgtttatta ggtggcaagt catggggtgc atgtgtaaat
     4261 agctacagaa atgcattggc acaaaatgca ggtgtctatt ccttcaatct gacattgtca
     4321 tacaacccga taaccacaac ctgcaaacag gacgatttat taattacttt agacagtatt
     4381 cccgttttac aattaccagc cacaggtaac aaagcaacaa taaatagtaa aaaaagggat
     4441 attattctgc gttgtaaaaa tttattaggt caacaaaatc aaacatcacg gaaaatgcag
     4501 gtgtatttat caagttctga cttgttaacc aacagcaaca caatactgaa aggtgcggaa
     4561 gataatgcgt aggatttatt cttgaaagta atggttcgcc agtcacactt ttaaatatca
     4621 ctaacagcag taaaggatac acaaatttaa aagaaattgc ggcaaagtca aaacttacag
     4681 atacaacggt ttcaattccg ataacagcca gttactacgt ctacgataca aacaaagtta
     4741 aatctggcgc actggaggca accgcattaa tcaacgtgaa atacgactaa taacaagcaa
     4801 aacgagcaag tggctaataa taaaaaataa caaggtgtct gcatttcccc ctcatgatga
     4861 gggggctttt tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat gattttaccg
     4921 tcggtgtcgc tgatcaacac gtacgcgcca cccatatagg tccagtgcgt cccggcatca
     4981 ggcgcgggca ggttacgtaa ctgccactgc ttaatgttgt actcatcggt gcggtacatc
     5041 tctgggacgg tatcacctgg tttgaaatgc ttaaagtcag caatgaaact acttaactca
     5101 tacttagaaa ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc ccaggcggca
     5161 ctggtaacca gcaaaagcac acccagcaac atcttatttt taatggtcat aaactctcca
     5221 ctttggggat ctgtaatatc agtcgcatat ctttcctgtc atacaatata actgacaagc
     5281 gatattttgt tagtgatccc atgatttcgt aaaaagtgta aatgaaagga tgccctaaaa
     5341 gcatccttag cgagcagaga gtcagcggaa atagctgatt taagaatcat cctgggctga
     5401 agataacatg caagcctaaa ggattgagtt atcgcattat gcccatgaag ccatgtacgc
     5461 ccatatacac accgactaag ccaatcaaca gactggaaaa atagggggcg cgtttagcga
     5521 gagtgttaaa tccgctccag cgttttgcga cctgctgaac gctgattgct gcgccaacgc
     5581 ctacggtgac aagcgttaac gccaggccaa tgctgaaact gacgaccagt gttgcgccca
     5641 gtgtcagggc tttcaactga atgcatatca acagcacggt aattgctgcc gggcagggga
     5701 taaagccacc ggttaagcca aataacaaaa tttgccagtt gatgacctct ctaccatcaa
     5761 agcgtcgttt aatgtcattg gcatgggctc gtgcatgggc atcctgatac tcgccatgtt
     5821 cgtgatggtg atgatgtcct tggtcgtggt gatgttcgtg atcgtgatga tggtgatgat
     5881 gctcataatc atgcccgtgc atattctcca gccagttgcg ttcgccgcgc taggtacgcc
     5941 agaacatcca gaacgcggtg ccaataatga tcactgcgga aatcagctgg agccacggtt
     6001 ctgctgattg agcagtaaag cgcttactga tcaccatccc gccaaaggca atcaaccaga
     6061 ccaccgcggt atgcgaaatg gttgctgcca gtccgagcat caccgcctgt ttaatggtgc
     6121 ctttgatggc gatgataaac gccgccatca tcgtttttga gtgccctggt tccaggccat
     6181 gaagcgcacc aagtaagatg gcgctgggga tgaagaacca ggcgtttcct tgctgaagaa
     6241 gagttgtaaa ttcggtcatg ataataattc ttagtattaa ttcggcaatc tgattctact
     6301 cccccccagt acctgatact acccccccag tagattaata gtgctatgat ttttcatatt
     6361 cttgttaacc aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc
     6421 gtgccagtaa gattcagggc caggtcgtgg cgctcaagaa aatgctcgac gagccgcacg
     6481 aatgcgctgc agttttacaa cagattgctg ctatccgtgg agcggtaaac ggtctgatgc
     6541 gggaagtgat taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac
     6601 gtgaagaaga tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta
     6661 acgcgattgt aaaactgccg tttttcctcg tttacaacgg gggcgctgga cattaccatc
     6721 ctcctctgcg atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg
     6781 agaaataccc gtatcacctg atctggataa tgccagcgta gggaagtcac ggaccaccag
     6841 gtcattgctt cttcacgtta tggcaggagc aaactatgca agtcgacctg ctgagttcag
     6901 cgcaatctgc gcacgcgtta cacctttttc accaacattc ccctcttgtg cactgcatga
     6961 ccaatgatgt ggtgcaaacc tttaccgcca ataccttgct ggcgctcggt gcatcgccag
     7021 cgatggttat cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt gccttgttga
     7081 ttaacgttgg cacactgacg cagccacgcg cacaggcgat gcgtgctgcc gttgagcaag
     7141 caaaaagctc tcaaacacgc tggacgcttg atccagtagc ggttggtgcg ctcgattatc
     7201 gccgccattt ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt ggtaatgctt
     7261 cggaaatcat ggcattagct ggcattgcta atggcggacg gggagtggat accactgacg
     7321 ccgcagctaa cgcgataccc gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg
     7381 tggtcactgg cgaggtggat tatgttaccg atggacatcg tatcattggt attcacggtg
     7441 gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg
     7501 cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt cactggatga
     7561 aacaagccgg agaacgcgca gtcgccagaa gcgaggggcc aggcagtttt gttccacatt
     7621 tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct
     7681 gacgattgcc ggtactgatc cgagtggtgg tgcggggatt caggccgatc ttaaaacctt
     7741 ctcggcactt ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg
     7801 tggcgtacag tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt
     7861 gttcagcgat gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa ccgatattgt
     7921 tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt
     7981 tatgctggca aaaagcggcg acccgctgct ttcaccttcg gcggttgcta cgctgcgcag
     8041 tcgattattg ccacaggttt cattaataac gccaaacttg cccgaagctg ccgccttgct
     8101 cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa caagggcgat cgctgttggc
     8161 gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga
     8221 ctggctgttt acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa
     8281 cacccacggc actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac
     8341 aaactgggct gacaccgtac aggaggcaaa aatctggctt tcatcggcgt tagcccaggc
     8401 cgacacgctg gaagttggtc acggtattgg tccggttcac cacttccacg cctggtggtg
     8461 agcaccgtac tatagctgct ttggttattg tcagcctttg gcactgacat tcagggatga
     8521 aaccaatgca gctaagaatt accagtcgaa aaaaactcac cgctctatta tgcgctcttg
     8581 ggctaatttc catcgttgca atttatccgc gtcagacggt gaattttttc tattcgacag
     8641 cagttcagat tacagactac atccacttct acggttatcg cccggttaaa tctttcgcta
     8701 ttcgcattcc tgccagttac accattcacg gattagatgt ttcgcgctgg caggagcgta
     8761 tcgactggca gcgggtggca aaaatgcgcg acaacgatat ccgcttacag tttgctttta
     8821 ttaaggctac ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac tggcaattaa
     8881 gccgcgaaaa tggcctgctg cgcggggcgt atcattattt ctccccgtcg gtatccgctt
     8941 cagttcaggc gcgattattt ctgcaaacgg tggatttctc acaaggcgat ttccctgccg
     9001 tgctggacgt agaagaacgg ggaaaattat cggcaaaaga attacgcaag cgggtaagtc
     9061 agtggctaaa aatggtcgaa aaacgcacgg gaaaaaagcc gattatttac tcaggagccg
     9121 ttttttatca caccaatctg gcgggctatt tcaatgaata tccgtggtgg gtggctcact
     9181 attatcaacg tcgtccggac aatgacggca tggcctggcg cttctggcag cattccgacc
     9241 gtggacaggt agatggcatc aatggtccgg tggattttaa tgtgtttaat ggcacgggga
     9301 tgagttgcag gcattcgttg atgggattaa agaaacgcct taaataagaa gcagtgatat
     9361 actcccacca gactccctgg caggaaaaag acaaatggaa caagcgcata cccagctgat
     9421 tgcccaactg aacgagcgta ttttagcggc ggataacacg ccgctttata ttaagtttgc
     9481 cgaaacggta aaaaatgccg tgcgcagcgg ggtgctggag catggcaata ttttgcccgg
     9541 tgagcgtgat ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca
     9601 ggcgctggaa gaagagggcg tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa
     9661 catcttcgaa tactcgttga aagaagcgag aggtttttct cagcaggtgg tattgcgcgg
     9721 gaaaaagccc gatacgctat gggttaataa gcgtgtcgtg aaatgtcccg aagaagttgc
     9781 gcagcagctg gcggccgaag cgggaagtga tgtctttttg cttaagcgta ttcgctatgt
     9841 cgatgaagaa gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt
     9901 tgatgccatc ggaatttctc tttatgacta tttccgcagc cagcacattt acccacaacg
     9961 tacgcgttcc cgcgttagcg cccggatgcc ggatgccgag tttcagtcac atattcagtt
    10021 agatagcaaa ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc
    10081 tattgagtac agcattagcc actgtcgcag cgatctatac gtttttgtgt gcgaggagta
    10141 gttcctcgcg cgttggcgca caatcaccgc cccgatgccc gacaacccac gacgccactg
    10201 cattgcccag taatacggca tccgccagtg gcagaccaga ggccagcccg gcaagtacgc
    10261 cacctgcatg actgtcgccc gccccaatgg tgtctacaac ttgcgttgga aatgccggaa
    10321 tgcagccaga agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga cgaacgatca
    10381 acggcgcggc aaatttctcc tgccattgct tgccaagtgt tgttatctct gcggataaag
    10441 caaaacgttc ggcggcaatc tcagcctctt gacgattgag tgacactaaa ggtcgacagg
    10501 ccatgatccg cgccagtaat gcgtccggga tatcgccaat acgtgggcaa aaatcgataa
    10561 acggcgtcac gtcttgcagc ttttccagcc attccactaa caattcgccg cagggcgagg
    10621 ccagttgata accggaaaaa tagagcaggt tgccaggcgc aacggttaat cgtgccaacc
    10681 attggcgatt ccattgattt tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg
    10741 gctcaaccag tgccagacac cagccgttat cgctttcggc gttatcgatc aggctgatta
    10801 acccctcttt cgccatccgg ttgcgaataa tctccgccca cacgccctga ccgagcggca
    10861 aggcattacc cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc
    10921 cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca cccacgccag gggagggcat
    10981 aagcgtcggc gatcacgtca atgaccgccg cgccgacgac catcaccggc tgacgcgtgg
    11041 ttaattcagg cagcagtgtg tgtaatcgag cgccgctcat accgcctccc gttgttgacg
    11101 atatttcgcc agcgctgtgg catagcggtt gaaatcaagc tgatttaccg catccagctc
    11161 cgcctttaac gcaggatcga cagcgttaat gccatgcaac gcgccgcaaa tcgccgtcgc
    11221 catagcacct atggtgtctg tgtcgccgcc aagattggcg cacaggacgg cgcagcggtt
    11281 tgggtcggtt tgtgccagtt caactagcgc aatggcgcac ggaacggact caatagtgct
    11341 ggtgcctgcg ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt
    11401 acgcacaatt ttcagcgcaa tctccagacg tgccgccagt gaggcgctga acgtggtggt
    11461 gcgtttttgc tgtgcatgtc gcgcaattga aggcaatgaa tcaacaatcg ctgaccagcg
    11521 ttctccgtca atggcacgag aaatcgccca tgcgatgact accgcgcctg caaccgccag
    11581 atcggattta tgggtcgggc tggacgccag cgccacatca tcaataaagg aatcaacatc
    11641 acgcgctgga agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt tggttacgcc
    11701 gttgttttcc agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga
    11761 ggtcggacct agtacgtttt tgttaaaggc gtcgaaacgc aacgcccagt cgagaatatt
    11821 acgcccaatc agattcggat cgatcttgcc ttcacattcc agtaacgcat ccgccagaca
    11881 caggccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    11941 tcctttggtc caggcagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    12001 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    12061 agaatacgtt ctgttttcat ttaacttccc cttgaatcgg aatttacggg agcatagtaa
    12121 tgacagatat tgcgcagttg cttggcaaag acgccgacaa ccttttacag caccgttgta
    12181 tgactattcc ttctgaccag ctttatctcc ccggacatga ctacgtagac cgcgtgatga
    12241 ttgacaataa tcgcccgcca gcggtgttac gtaatatgca gacgttgtac aacaccgggc
    12301 gtctggctgg cacaggatat ctttctattc tgccggttga ccagggcgtt gagcactctg
    12361 ccggagcttc atttgctgct aacccgctct actttgaccc gaaaaacatt gttgaactgg
    12421 cgatcgaagc gggctgtaac tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc
    12481 ggcgctatgc gcatcgcatt ccatttctcg tcaaacttaa tcacaacgag acgctaagtt
    12541 acccgaatac ctacgatcaa acgctgtatg ccagcgtgga gcaggccttc aacatgggcg
    12601 cggtggcggt tggtgcgact atctattttg gctcggaaga gtcacgtcgc cagattgaag
    12661 aaatttctgc ggcctttgaa cgcgcgcacg agctgggcat ggtgacagtg ctgtgggcct
    12721 atttgcgaaa ctccgccttt aagaaagatg gcgttgatta ccatgtttcc gccgacctga
    12781 ctggtcaggc gaaccatctg gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg
    12841 cggaaaataa cggcggctat aaagcgatta attacggtta taccgacgat cgcgtgtaca
    12901 gcaagttaac cagcgaaaac ccgattgatc tggtgcgtta tcagttagct aactgctata
    12961 tgggccgggc tgggttgata aactccggcg gtgctgcagg cggtgaaact gacctcagcg
    13021 atgcagtgcg tactgcggtt atcaacaaac gcgcaggcgg aatggggctg attcttggac
    13081 gtaaggcgtt caagaaatcg atggctgacg gcgtgaaact gattaatgcc gtgcaggacg
    13141 tttatctcga tagcaaaatt actattgcct gatacgcctc atacgaacag caccaccccg
    13201 taggacggat aagacgttta cactgcatcc ggcaacctat gcctgatgcg acgcggaagc
    13261 gtcttatcat gcctacatag cactgccacg tatgtttaca ccgcatccgg cataaaaaca
    13321 cgcgcacttt gctacggctt ccctatcggg aggccgtttt tttgcctttc actcctcgaa
    13381 caattttcat attgttgttt ttgcgatcat tatctcgaca tttaaaagca aataatttca
    13441 ttatattttg aaatcgaaag caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag
    13501 cagatgctga acaacgcaca gcgcggcggt tatgcggttc cggcattcaa tattcacaat
    13561 ctcgaaacga tgcaagtggt ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc
    13621 gccggaacgc ctggcacatt tactcatgct ggtacagaaa atctgttggc gctggtcagc
    13681 gcgatggcga agcaatatca ccatccactg gcaattcatc tcgaccatca cacgaaattt
    13741 gacgatatcg ctcagaaggt tcgttctggc gtgcgctcag tcatgattga cgcctcgcat
    13801 ttgccttttg cgcaaaatat ttcacgggtc aaagaggtgg tggatttttg ccatcgcttt
    13861 gatgtcagcg tcgaggcgga gctggggcaa cttggcggcc aggaagatga tgtgcaagtc
    13921 aatgaagtcg atgcgttgta caccaacccc gcacaggcgc gtgaatttgc cgaggcaacc
    13981 gggattgatt ccctggcggt cgccatcggc acggctcacg gtatgtatgc cagcgcaccg
    14041 gtgcttgatt tttctcgact ggagaacatt cgccagtggg tgaacttacc gctggtgctg
    14101 catggcgcgt caggattatc gactaaggat attcagcaaa ccatcaaact ggggatatgc
    14161 aaaatcaacg ttgcaacgga gctgaaaaat gccttcttgc agtcgttaaa aaattacctg
    14221 accgagcacc ccgaagcgac cgatccccgg gattatttgc agtcggctaa atccgcaatg
    14281 cgcgatgtgg tgagcaaagt gattgccgat tgtggctgcg agggcagggc ataacgcacc
    14341 tgccatttaa caaggaaaaa acatgaaaac gttaattgcc cggcataaag ctggtgaaca
    14401 tatcggcata tgttcagtct gttctgccca tccgttggtt atcgaagcgg cgctggcatt
    14461 tgatcgcaac agcacgcgca aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt
    14521 tggcggttat accggaatga caccggcaga ctttcgcgaa tttgttttta cgattgccga
    14581 taaagttggg tttgcacgcg aacgcattat tctcggcggc gatcatctgg gaccaaactg
    14641 ctggcagcaa gaaaatgcgg atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata
    14701 tgttcgtgct ggcttcagta aaattcatct tgatgcgtca atgtcctgcg cgggggatac
    14761 cataccgtta gcaccagaaa cggttgcgga acgagctgct gtgctttgct ttgctgcgga
    14821 aagtgtggcg acagattgcc agcgtgagca actgagctat gtcattggca ctgaagttcc
    14881 ggttccgggc ggtgaggcca gcgccattca gtcagtacac atcacccatg ttgaagatgc
    14941 cgccaatact ttacgtacgc atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt
    15001 aacacgtgtg attgccatcg tggtgcagcc gggtgtggaa tttgatcaca gcaatattat
    15061 ccattatcag ccgcaggaag cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt
    15121 ttatgaagca cattctaccg attaccagac ccggacggct tattgggaat tagtccgcga
    15181 tcactttgca atattgaaag tcggtcccgc attaaccttt gctttacgcg aggcgatatt
    15241 tgcactggca caaattgagc aggaacttat cgcccccgaa aatcgcagcg gttgcctggc
    15301 ggtaattgaa gaagtgatgc tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg
    15361 ttttaacgat tcattactgg atattcgtta cagcctgtcg gatcgtattc gttattactg
    15421 gccgcatagt cggattaaaa atagcgttga aacgatgatg gtgaatctgc aaggcgtgga
    15481 catcccactg ggcatgatta gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg
    15541 ggaattatca gcaatgccgc atcagcttat tatgaataaa atttatgatg ttttgcgcgc
    15601 ctatcgctac ggctgtgcgg aataaggacg gtatatgact aacctgtttg ttcgtagcgg
    15661 aatttctttt gtcgatcgta gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa
    15721 aggtgtggtt catgatacct ggccacaggc attaattgcc agagaagcag aattccctac
    15781 cgggataatg cttgagcagc acgccattgc aataccgcat tgtgaggcga ttcatgctaa
    15841 gtcgtcagcc atttatctgt taaggccaac aaataaagtt cattttcagc aagcggatga
    15901 tgataacgac gtggcggtat cgttggttat tgcgttgatt gtggaaaatc cgcagcagca
    15961 attgaaactt ttacgctgtt tatttggcaa gttacaacag cccgatatcg tcgagacact
    16021 aattactctt cctgaaactc agttaaagga atacttcaca aagtatgttt tagattcaga
    16081 cgaataaatc cctctgtaac aataaaaagg actatttatg aaacgcaaga ttattgtcgc
    16141 ttgcggaggc gcggttgcga cctctacgat ggcggcggaa gaaattaaag agttgtgtca
    16201 gagtcataat attcctgttg aattaatcca gtgtcgggtt aatgaaatag aaacctatat
    16261 ggatggtgtg catttgatat gtaccactgc cagggtggat cgtagttttg gcgatattcc
    16321 gttagttcac ggcatgcctt ttgtttctgg tgtcggtatc gaagcattac aaaataaaat
    16381 tctgactatc ttacagggat gacctatgtt ttcagaagtc atgcgttata ttctcgacct
    16441 cggccctaca gtgatgctgc cggttgtcat cattattttt tctaaaatat taggcatgaa
    16501 ggcaggcgat tgctttaaag cgggtctgca tatcgggatt ggctttgttg gcattggcct
    16561 tgtgattggc ttaatgctgg attccattgg tccggcggcg aaagcgatgg cggaaaattt
    16621 cgacctgaat ctgcatgtgg tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc
    16681 gtcgcaaatt gcgctggtgg cgattccgat tgcgatattg gttaacgtgg cgatgttact
    16741 gacccgtatg acgcgggtgg taaatgttga tatctggaat atctggcata tgaccttcac
    16801 cggcgcgttg ctgcacctgg caaccggttc atggatgata gggatggcgg gtgtggtaat
    16861 tcacgcggcg tttgtttata agctcggcga ctggtttgcc cgcgataccc gaaatttctt
    16921 tgagctggaa ggtattgcta ttccgcacgg tacgtcggcg tatatggggc cgattgcggt
    16981 gctggtcgat gctatcatcg agaaaatccc aggcgttaac cgaattaaat ttagcgccga
    17041 cgatattcag cgcaaatttg gcccttttgg cgaacctgtc accgtgggtt ttgtgatggg
    17101 gctgattatc ggcatcctcg cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa
    17161 aacggcggca gttatgctgc tgatgccacg gatgattaaa cccatcatgg atggtttaac
    17221 gcccatcgct aagcaggccc gtagtcgttt acaggcgaag ttcggcggtc aggagttcct
    17281 gattggcctg gatcccgcat tactgctggg gcatacggcg gtggtatcgg caagcctgat
    17341 ttttattccg ctcaccattt taattgctgt ttgtgttccg ggtaatcagg tactgccgtt
    17401 tggcgatctt gccactatcg gcttcttcgt ggcgatggcg gtcgcggtgc atcgtggaaa
    17461 tctgttccgc accttaatct cgggtgtcat cattatgagc atcaccctgt ggatcgcgac
    17521 gcaaactatt ggtttgcaca cccaaccggc ggctaatgct ggggcgttaa aagccggggg
    17581 tatggtggct tcaatggatc agggcggttc tcccattacc tggttactga ttcaggtttt
    17641 ctccccgcaa aatattcccg gtttcattat tatcggcgca atttatctga ccggtatttt
    17701 catgacctgg cgtagagcgc gtggttttac taaacaagag aaagccgttc tcgcagaata
    17761 atttttacct gagggggagg ttatccccct taacaa
//
LOCUS       DAZOZX010000078        17757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZOZX010000078 DAZOZX010000000
VERSION     DAZOZX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.078"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            153..836
                     /locus_tag="ACHUPT_002593"
     CDS             153..836
                     /locus_tag="ACHUPT_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0820124.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            880..5542
                     /locus_tag="ACHUPT_002594"
                     /pseudo
     CDS             880..5542
                     /locus_tag="ACHUPT_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5910..7565
                     /gene="lldP"
                     /locus_tag="ACHUPT_002595"
     CDS             5910..7565
                     /gene="lldP"
                     /locus_tag="ACHUPT_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0820125.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7565..8341
                     /gene="lldR"
                     /locus_tag="ACHUPT_002596"
     CDS             7565..8341
                     /gene="lldR"
                     /locus_tag="ACHUPT_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0820126.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8338..9528
                     /gene="lldD"
                     /locus_tag="ACHUPT_002597"
     CDS             8338..9528
                     /gene="lldD"
                     /locus_tag="ACHUPT_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0820127.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9724..10197
                     /gene="trmL"
                     /locus_tag="ACHUPT_002598"
     CDS             9724..10197
                     /gene="trmL"
                     /locus_tag="ACHUPT_002598"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0820128.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10250..11071)
                     /gene="cysE"
                     /locus_tag="ACHUPT_002599"
     CDS             complement(10250..11071)
                     /gene="cysE"
                     /locus_tag="ACHUPT_002599"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0820129.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGCGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11151..12170)
                     /gene="gpsA"
                     /locus_tag="ACHUPT_002600"
     CDS             complement(11151..12170)
                     /gene="gpsA"
                     /locus_tag="ACHUPT_002600"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0820130.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12170..12637)
                     /gene="secB"
                     /locus_tag="ACHUPT_002601"
     CDS             complement(12170..12637)
                     /gene="secB"
                     /locus_tag="ACHUPT_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0820131.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12700..12951)
                     /gene="grxC"
                     /locus_tag="ACHUPT_002602"
     CDS             complement(12700..12951)
                     /gene="grxC"
                     /locus_tag="ACHUPT_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0820132.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13093..13524)
                     /locus_tag="ACHUPT_002603"
     CDS             complement(13093..13524)
                     /locus_tag="ACHUPT_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0820133.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13769..15313
                     /gene="gpmM"
                     /locus_tag="ACHUPT_002604"
                     /gene_synonym="pgmI"
     CDS             13769..15313
                     /gene="gpmM"
                     /locus_tag="ACHUPT_002604"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0820134.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15347..16606
                     /gene="envC"
                     /locus_tag="ACHUPT_002605"
     CDS             15347..16606
                     /gene="envC"
                     /locus_tag="ACHUPT_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0820135.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16610..17569
                     /locus_tag="ACHUPT_002606"
     CDS             16610..17569
                     /locus_tag="ACHUPT_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR0820136.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17556..17757)
                     /locus_tag="ACHUPT_002607"
                     /pseudo
     CDS             complement(17556..17757)
                     /locus_tag="ACHUPT_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4444 a         4614 c         4645 g         4054 t
ORIGIN      
        1 aaacgaatga tacattttta tgatgaatgc ttaaaacaat atttaatcga cacacatcac
       61 tgtcattaat gtaaagcaac atagctcacc aataatacaa tggacaactc taccggcatt
      121 gagcgctggt cggaggaaat aaggattttt acatgaactt gaagaagatt tttttcagtg
      181 ctgtcactgt ttcagttctc tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc
      241 cagaaagtag tttactgaaa caacaagaag aacataataa taaaattgtg ctacttgaaa
      301 aacaacaagc acagctaaag agtcaactcg aaactattca aaaacaacaa actggcataa
      361 taaacagtac aaagacttta actcacgtaa ttaaatccgt taaagaccaa caaaatactt
      421 ttatttttac cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aacggttcgg
      481 tggctttagc gggtcgagtg ttatccattg acgccacaga gaatggtagt gttattcata
      541 tctcactggt caacttatta agtacaccta tctcaaacat tggttttaat gcgacatggg
      601 gtggtgaaaa acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca
      661 catcaatgaa atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc
      721 tgaagggtgt atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata
      781 ttcagttcga caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc
      841 agttagtacg cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt
      901 atctggaacc ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc
      961 aagaaatctg ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg
     1021 tcgtttgggg cattggcgaa tgccgggaat gacaacggtc agggtgttga ttacggtagt
     1081 ggatcagctg gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg
     1141 aacaccagtg gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc
     1201 tctgccatcg ggtcaaaaac ccatgcgatt ggtggtgcat caatggcctt tggggttagt
     1261 gcaatatcag aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa
     1321 tactcaatgg ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg
     1381 gactcttcca aagcggaagg agcaaacgcc attgccctgg ggcgtagcag tgtagctagc
     1441 ggtactgaca gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa cgctattgcg
     1501 ataggtgctg agaccgaagc cgctgaaaat gcaactgcta ttggcaataa tgcgaaggca
     1561 aaagggacta atagcatggc aatggggttc ggaagccttg ccgataaagt caatactatc
     1621 gcattaggaa atggcagcca ggctctggca gataatgcaa tcgccatagg ccagggcaac
     1681 aaagctgatg gcgtggatgc catcgctctg ggtaatggta gccagtcgag aggcttaaac
     1741 accattgcct taggcacagc cagtaatgca actggtgata agagtcttgc gcttggtagt
     1801 aatagcagtg ccaacggtat taactctgtc gcgctgggcg cagattccat tgcggattta
     1861 gacaataccg tctctgtcgg caatagttca ttaaaacgca agatcgttaa tgtgaaaaat
     1921 ggcgcgatca agtctgacag ttacgatgcc attaatggtt cacagcttta tgccattagc
     1981 gactcggtag caaaaaaggc ttggaggagg ggctgcagta gatgttgatg acggtactgt
     2041 tacagcacca acctacaatt taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc
     2101 tgtacttgat gaaaacaccc tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca
     2161 tggtactagt agcccaactg ccagcgtaat caccgatgtt gcggatggca cgatttcagc
     2221 ctccagtaag gatgcggtta acggttccca actgaaagct accaatgacg atgtcgaagc
     2281 caacaccacc aatatcacca acctgacgga ttccgttggt gaccttcagg ctgatgccct
     2341 gctctggaac gaaactaaaa aggcattcag tgcagctcac ggccaggata ccaccagcaa
     2401 aatcaccaac gttaaagatg ccgacctgac ggctgacagc actgatgctg ttaacggctc
     2461 tcagctgaaa accaccaacg atgctgtggc gacgaatacc accaatatcg ccaataacac
     2521 ttccaatatt gccactaaca ccaccaacat ctctaacctg actgagacgg tgactaatct
     2581 tggtgaggat gcgctgaaat gggataagga caatggtgta ttcacggcag ctcatggcac
     2641 cgagaccacc agcaaaataa ccaacgttaa agatggcgac ctgacgactg gcagcaccga
     2701 tgccgttaac ggctctcagc tgaaaaccac caacgatgcc gtggcgacga ataccaccaa
     2761 tatcgccact aacaccacca acatctctaa tctgactgag acggtgacta atcttggtga
     2821 ggatgcgctg aaatgggata aggacaatgg tgtcttcact gcagctcatg gcaacaatac
     2881 cgccagcaaa atcaccaata tcctggacgg cacagtcact gcaaccagtt ccgatgccat
     2941 taacggtagc cagctttatg acttaagcag caatatcgcc acctacttcg gcggcaatgc
     3001 ttctgtgaat actgacggtg tgtttaccgg tccaacctac aaaatcggtg aaacaaatta
     3061 ttataacgtc ggcgatgcac tggctgcgat taactcctca tttagcacgt ctctcggcga
     3121 tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc aaacacggta ctaatggtga
     3181 cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca gactccagtt ctgacgcagt
     3241 aaacggctca caactccacg gcgtgagcag ttatgttgtt gatgcgctgg ggggtggtgc
     3301 cgaagtcaat gcagacggca ccatcactgc gccgacgtac accattgcta atgctgatta
     3361 cgataatgtc ggtgatgccc tgaatgctat cgataccact cctgacgacg ctctgctctg
     3421 ggatgcggac gccggtgaaa atggtgcatt tagcgccgct cacggaaaag ataaaactgc
     3481 cagtgtaatc actaacgtcg ctaacggtgc aatctctgct gccagcagcg acgcgattaa
     3541 cggctcacaa ctctatacca ccaataagta catcgctgat gcgctgggtg gtgacgcaga
     3601 agtcaacgct gacggcacca tcaccgcacc gacttacacc attgcgaacg ccgagtacaa
     3661 caacgtcggt gacgccctgg atgcgcttga tgataacgcc ctgctgtggg ataagactgc
     3721 caatggcggt gctggagcct acaatgccag ccatgacggt aaagccagca tcatcactaa
     3781 tgtcgctaat ggcagtatta gtgaggacag taccgatgca gtgaacggtt ctcagttgaa
     3841 tgcgacgaat atgatgattg agcagaacac ccaaattatc aatcagctcg ctggtaacac
     3901 cgacgcaacc tatatcgaag aaaatggcgc tggtatcaac tacgttcgta ctaacgacaa
     3961 cgatttagcc tttaacgatg caagcgcctc tggtgtcggc gctacagctg taggttataa
     4021 cgctgtcgcg tctggtgcca gcagcgtagc cattggtcag aacagcagca gcaccgttga
     4081 taccggtatt gcgctgggta gcagctccgt ttccagccgt gtgattgcca aaggttctcg
     4141 tgacactagc gtaacggaaa atggcgtggt gattggttat gacaccactg acggcgaact
     4201 gctaggtgca ttgtcaattg gtgatgacgg taaataccgc caaatcatca acgtagctga
     4261 tggttcagaa gcccatgacg ccgttacggt tcgccagttg cagaacgcta ttggagcggt
     4321 cgccactacg ccaaccaagt actttcacgc caactcaacg gcagaagact cactggccgt
     4381 tggtgaagac tcgctggcaa tgggtgcgaa aactgtcgtt aatggtaatg caggtattgg
     4441 tatcggtttg aacactctgg ttctggctga tgcgatcaac ggcattgcta tcggcagtaa
     4501 cgcacgcgca aaccatgcaa acagtatcgc aatgggtaac ggttctcaga ccacccgtgg
     4561 tgcacagact ggctacaccg cctacaacat ggacgcaccg cagaactctg taggtgagtt
     4621 ttctgtcggt agtgaagacg gtcaacgtca gatcacaaac gtcgcagctg gttcagcgga
     4681 taccgatgcg gttaacgtgg gtcagttgaa agtcactgat gagcgcgtag cgcaaaatac
     4741 ccagagcatt actaacctga acaatcaggt cactaatctg gatactcgcg ttactaatat
     4801 cgaaaacggt attggcgaca ttgtcaccac cggtagcacc aagtacttca agaccaacac
     4861 cgatggcgta gatgccaacg cccagggtaa agatagcgtt gctattggtt ctggttccat
     4921 tgctgccgct gacaacagcg tcgcactggg taccggttcc gttgcagagg aagaaaatac
     4981 aatctctgta ggttcttcta ctaaccaacg ccgtatcacc aacgtagctg ccagtgttaa
     5041 tgccaccgat gcggttaacg tgtcgcagct gaaatcttct gaagcaggcg gagtgcgcta
     5101 cgacaccaaa gctgatggtt ctatcgacta tagcaatatc accctcggtg gtggcaacgg
     5161 cagtacgact cgtatcagca acgtctccgc tggcgtcaac aacaacgacg cggtgaacta
     5221 cgcgcagttg aagcaaagcg cgcaggaaac gaagcaatac accgatcagc ggatggttga
     5281 gatggataac aaactgtcta aaactgaaag caagttgagc ggtggtatcg cttctgcaat
     5341 ggcaatgacc ggtctgccgc aggcttatac accgggtgcc agcatggctt ctattggtgg
     5401 cggtacttac aacggtgaat cggcagttgc tttaggtgta tcgatggtga gcgccaatgg
     5461 tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact ccgccgcact
     5521 cggtgccggt attcagtggt aatgatccat taagttagtg tgactaaggg caaacgccaa
     5581 attgcctgat gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa caatctaagg
     5641 gcaattctct gatgaggatt gcccttttct ttaccagaca tctcccccca caagaattgg
     5701 ccctaccaat tcttcgctta tctgacctct ggttcacaat tccccaatta aaactcacat
     5761 caatattgcc aatacataac atttagttaa ccattcattg tcattatccc tacacaacac
     5821 aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag actcattaca
     5881 cgatgtgcgt ggactctagg agacctgcaa tgaatctctg gcaacaaaac tacgatcccg
     5941 ccgggaatat ctggctttcc agtctgatag catcgcttcc catcctgttt ttcttctttg
     6001 cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggta gcaatcgccc
     6061 ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt
     6121 atggcttctt ctatggtttg tggcccatcg cgtggatcat tattgcagca gtgttcgtct
     6181 ataagatctc ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt ctttcgataa
     6241 cccctgacca gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct ttccttgaag
     6301 gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc ggcctgggtt
     6361 ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca gtggcatttg
     6421 gcgcgatggg cattccaatc ctggttgccg gacaagtaac aggcatcgac agctttgaga
     6481 ttggccagat ggtggggcgt cagctaccgt ttatgaccat tatcgtgctg ttctggatca
     6541 tggcgattat ggacggctgg cgcggtatca aagagacgtg gcctgcggtc gtggttgcag
     6601 gcggctcgtt tgccatcgct cagtacctta gctctaactt cattgggccg gagctgccgg
     6661 acattatctc ttcgctggta tcactgctct gcctgacgct gttcctcaaa cgctggcaac
     6721 cagttcgcgt attccgcttc ggtgacttgg gggcgtcaca ggttgatatg acgctggccc
     6781 acaccggtta caccgcgggt caggtgttac gtgcctggac accgttcctg ttcctgaccg
     6841 ctaccgtaac actgtggagc atcccgccgt ttaaagccct gttcgcatcg ggtggtgcgc
     6901 tgtatgagtg ggtgatcaac attccggtgc cgtacctcaa taaactggtt gcccgtatgc
     6961 caccagtggt cagcgaggct acagcctatg ccgccgtgtt taagtttgac tggttctctg
     7021 ccaccggcac cgccattctg tttgctgcac tgctctcgat tgtctggctg aagatgaaac
     7081 cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga actggctcta cccatctact
     7141 ccatcggcat ggtgctggcg ttcgccttta tctcgaacta ttccggactg tcatcaacac
     7201 tggcactggc actggcgcac accggtcatg cattcacctt cttctcgccg ttcctcggct
     7261 ggctgggggt attcctgacc gggtcggata cctcatctaa cgccctgttc gccgcgctgc
     7321 aagccaccgc agcacaacaa attggcgtct ctgatctgtt gctggttgcc gccaatacca
     7381 ccggtggcgt caccggtaag atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg
     7441 taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac agcctgatct
     7501 tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg tggatgattc
     7561 cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct
     7621 gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc aactggcgat
     7681 gcaactcggc gtatcacgta attcactacg cgaggcgctg gcaaaactgg tgagcgaagg
     7741 cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg acacatggtc
     7801 ggagcaaaac atcgtccagc cgctaaaaac gctgatggcc gatgatccgg attacagttt
     7861 cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg cggcaatgcg
     7921 cgccacacct ggcgacaaag aaaagattca gctttgcttt gaagcaacgc taagtgaaga
     7981 cccggatatc gcctcacaag cggacgttcg ttttcatctg gcgattgccg aagcctcaca
     8041 taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat cctcagtgaa
     8101 gcatagccgt cagcggatgt atctggtgcc accggttttt tcacaactga ccgaacaaca
     8161 tcaggctgtc attgacgcca tttttgctgg tgatgccgac ggggcgcgta aagcaatgat
     8221 ggcgcacctt agttttgttc acaccaccat gaaacgattc gatgaagatc aggctcgcca
     8281 cgcacggatt acccgcctgc ccggtgagca taatgagcat tcgagggaga aaaacgcatg
     8341 attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg
     8401 ttccactata tggatggtgg tgcatattct gaatacacgc tgcgccgcaa cgtggaagat
     8461 ttgtcagaag tggcactgcg ccagcgtatt ctgaaaaaca tgtccgactt aagcctggaa
     8521 acgacgctgt ttaatgagaa attgtcgatg ccggtagcac tggcaccggt gggtttgtgt
     8581 ggcatgtatg cgcgtcgtgg cgaagttcag gcagccaaag cggcggacgc gcatggcatt
     8641 ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag
     8701 cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg
     8761 gagcgagcaa aagcagcggg ttgttcgacg ctggttttca ccgtggatat gccgacaccg
     8821 ggcgcacgct accgtgatgc gcattcaggt atgagcggcc caaatgcggc aatgcgccgc
     8881 tacttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtcca
     8941 catgatttag gtaatatctc agcttatctc ggcaaaccga ccggactgga agattacatc
     9001 ggctggctgg ggaataactt cgatccgtcc atctcatgga aagaccttga gtggatccgc
     9061 gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat
     9121 gcagtacgtt ttggtgctga tggaattgtg gtttctaacc acggtggccg ccagctggac
     9181 ggtgtactct cttccgctcg tgcgttgccc gctattgcag atgcggtgaa aggtgatatc
     9241 gccattctgg cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc
     9301 ggtgccgaca ccgtactgct gggtcgtgct ttcctgtatg cgctggcaac agcgggccag
     9361 gcgggtgtag ctaacctgct aaatctgatc gaaaaagaga tgaaagtggc gatgacgctg
     9421 actggcgcga aatcgatcag tgaaattacg caagattcgc tggtgcaggg gctgggtaaa
     9481 gagttgccta cggcactggc tccgatggcg aaagggaatg cggcatagtc gtttgcgccc
     9541 cctcacccta accctctccc tcagggagag gggaccgttc ggcgctgtat gtactccctc
     9601 actctgaaac gacaccgcac tctttttttc tccctcgccc ctccggggag ggccggggtg
     9661 aggggaaaag gccgcacggt tcaccaattt tctgctatcc tgcccccgca ctaagggggc
     9721 agtatgctaa acatcgtact ttacgaacca gaaattccgc caaatactgg caacatcatc
     9781 cgtctttgcg ctaataccgg ctttcgtcta catatcatcg aaccgatggg atttgcctgg
     9841 gacgacaagc gcctgcgccg cgcggggctg gactatcacg agtttaccgc cgttacgcgt
     9901 catcatgact atcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc
     9961 acgaaaggta cgcctgctca cagcgccgta agctatcagg atggcgatta tctgatgttc
    10021 ggcccggaaa cacgcggcct gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa
    10081 attcgcattc cgatggtgcc ggacagccgc agcatgaatc tgtccaatgc ggtgtcggta
    10141 gtggtgtatg aagcctggcg gcagctggga tatccgggag cgttgttgag agattagtta
    10201 ctgtatgccg gatagatgat tacatcgcat ccggcacgat cacaggacat tagatcccat
    10261 ccccatactc aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc attgatggct
    10321 tatcgctgtc tggtttaccg acaatacgag ccggaacgcc agcggcggtt gtatgcggcg
    10381 gcaccggttg cagcaccacg gaacctgcgc caatcttcgc gccgcaacca acttcaatat
    10441 tgccgaggat tttcgcgccc gcgccaatca tcacaccttc acgaattttc gggtgacggt
    10501 caccaccaga tttacccgta ccgccaagcg tcacagattg cagaatcgat acgtcgtttt
    10561 caattaccgc cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata atgccacggc
    10621 caatttttgc tgccgggtga atatcgacct ggaacgtcac agaaacctgg ttttgcagaa
    10681 agattgccaa tgcgcgacgc ccctgattcc acaaccagtg accgatgcga taggcctgca
    10741 aggcatgaaa acccttcagg tataacaacg gggttgagta tttatcgact gccgggtcgc
    10801 gggtacgcac cgcctgaata tcacaggccg cagaggcgat catttccggg tcagcggcgt
    10861 aggcttcttc aaccacttca cggatagcaa tagcaggcat aattggcgat gacagcttgt
    10921 tcgccagcat gtagctcagt gcactgccaa ggttttcgtg cttgagtagc gtcgcgtggt
    10981 aaaaactggc cagcattggc tcacagtccg ccagcgttct ggcttcggct ttaatattgt
    11041 tccagacaat ttccagttct tcacacgaca ttgcttactc cacacgatga gataatgacc
    11101 ggcccgttct gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg ttagtgactg
    11161 ctgcgctcgt ccttgcgtgc acgacctaat aatgtcaatg ctgcctcgcg cgcgtttttt
    11221 ccgcaatata atacttgata aatttcctcg gttattggca tttcaacgcc gaagcgatgc
    11281 gccagttcgc ggacttcttt cgtattgcgg tagccttcca ccacctgacc aatcttctcc
    11341 tgcgcgcttt gtacatccat gccctgaccg agcatcatac caaaacggcg gttacgcgac
    11401 tggttgtcgg tacaggtaag caccagatcg cccagccccg ccatgcccat aaaggtggca
    11461 ggatcggcac ccagcgccgc accaagacgc gacatttcag ccagcccacg ggtgatcagc
    11521 gccgtacgcg cattcgcacc aaaaccgata ccgtcagaca tccccgcgcc aatggcaatg
    11581 acgtttttca ccgcgccgcc aagctgcacg ccaatgaaat ccgggttgct gtaaacgcgg
    11641 aaacttttgc cgcagtgcag cagctgctgg agatcatcgg caaaggtctg atcggtcgag
    11701 gccagcgaaa tagctgtcgg taaacctgcc gccagttctt tcgcaaacgt tgggccagag
    11761 ataaccgcca gcggaatttg atcgcctaag gcctcacgcg ccacgtcctg taacagacgt
    11821 ccggtttccg cttccagccc tttggtcgcc cacaccagac gcgcatcagg acgcatcagc
    11881 ggtttaatct ggcgcagcac ttcaccaaag acatggctgg gtacgacgac gagaatatta
    11941 cgactggctg ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc
    12001 acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg
    12061 tcatggcccc agaggacaac ctcgtggcca tttcttgcca gggtaatggc aagagcggtg
    12121 ccgtacgagc cggcaccgat cacagtcatt gaagcattac gttggttcat caggcatcct
    12181 gatgttcttc agtaccttcg ccagcctgct gctgcaaata gttcatgaac agcgcatcga
    12241 agttaaccgg cgcaaggttc agttgcggga atgtaccgcg ggataccatg ctggtgatgc
    12301 actcacgagc atacgggaac agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct
    12361 gggtgccttc gatacccgcg atggagaaaa taccgccctg ctgaacttca cacaggaacg
    12421 cggtttcttc gcccaaggag gccgttacgg taacacgcag taccacttcg tatacgtcat
    12481 ctgccagttg ggaagatgcc gtatccagat caagtttaac ttctggttgc caatctttct
    12541 ggaaaacgtg cggcgcgttc ggcgcttcga aagagatatc cttggtataa atacgttgga
    12601 tctggaaagt catttcagtg ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg
    12661 tccttaaata ctgcgcaatg taataccaac gttcacacgt tatttcagca ggggatccag
    12721 tccaccacgt gcatccaatg catacaagtc atcacagccg ccaatgtgct gtgcgtcaat
    12781 aaaaatctgg ggaaccgtgg tgcaaccgct gcgtttgatc atctcttcac gcttggcggc
    12841 attgccatcg atcggcaact cctggaaact cacgcccttg ctgctcagca gtgcttttgc
    12901 acgatggcaa tacgggcagg tttctttggt atagatttca acattggcca taatttagct
    12961 cctgtttttt taccctgaaa tttcatgttg cagggcggct gcaaaatgga gtgtccccag
    13021 aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc atcatgaaag
    13081 acgacaggta aattatttgc cgcgcaccag aggcaagttt tcgccagccc agccagcaac
    13141 gccctctttc agcacgaata cttgcgcaaa accagctttc gtcagcgcgt ttgcaggctc
    13201 ctggcactgc atgccagaac cgtctaccac gataaccgat ttgtctttgt gcttctcaag
    13261 ctcaccaaca ttgttggctt tgatttcgct cggcaacaga ttgatagaac ctgcgatatg
    13321 gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt ctttgttgat
    13381 cagacgcgta gcttcaccac gagtaatcac cttcactttc gaggtcaggc tcttaaacgt
    13441 agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg gatgacggcc
    13501 aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg attaaaaaaa
    13561 ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta actggaatgc
    13621 aattttgcgg ggcacgacga aaaaaaagcg caaatctggc atcggcttgc cgcgagcggt
    13681 cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa ttacgcaaat
    13741 tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta tggtactggt
    13801 gattctggat ggctatggct atcgtgaaga acagcaggat aacgccattt ttagtgctaa
    13861 aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg acgcttccgg
    13921 tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc acgttaacct
    13981 gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa tcaaagatcg
    14041 cgctttcttt gctaatccag tgctggctgg cgcggtacat aaagcgaaaa acgcaggtaa
    14101 agcagtacac attatgggtc tgctctccgc aggcggcgta cacagccacg aagatcacat
    14161 catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc tgcacgcatt
    14221 ccttgacggt cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa aatttgaaga
    14281 gaaatttgct gcgctgggta aaggccgcgt agcgtccatc attggtcgtt actacgcgat
    14341 ggaccgcgat aaccgttggg atcgcgttga aaaagcctat gacctgctga ctctggcgca
    14401 gggtgagttc caggccgata ctgccgttgc tggtttgcag gcagcttatg ctcgcgacga
    14461 aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag atgcggcaat
    14521 ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc gtgaaatcac
    14581 tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg ttaacgtcga
    14641 tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt acccacccgc
    14701 ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc agttgcgtat
    14761 ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg tagaagagtc
    14821 gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct acgatctgca
    14881 accggaaatg agctccgcag agctgaccga aaaactggtt gcggccatca agagcggcaa
    14941 atacgacacc attatctgta actatccgaa cggcgacatg gtaggtcaca ccggggtgat
    15001 ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag tcgcgaaagc
    15061 ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg ctgaacagat
    15121 gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag ttccgctgat
    15181 ttacgttggt gataagaacg tgaaagcggt tgcaggcggc aaactttctg acatcgcgcc
    15241 gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta agccgctgtt
    15301 catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga cacgggccgt
    15361 gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta gcgctggcgt
    15421 attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat ctattcaggc
    15481 cgatatcgcc gcaaaagagc gcgcggtacg ccaaaagcaa caacaacgcg caagcctgct
    15541 cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc tgcgcgaaac
    15601 gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt cgattgccaa
    15661 actggagcag caaaaagccg cccaggagcg cagcctcgcc gcacaactgg atgccgcatt
    15721 ccgtcagggc gagcataccg gtattcagct aattctcagc ggtgaagaaa gccagcgtgg
    15781 acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa ccattgccca
    15841 gttgaagcaa acgcgtgaag aagtcaccat gcagcatgct gaactggaag agaaacaaag
    15901 cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaaactga ctcaggctct
    15961 gaacgagcgt aaaaagacgc tggcggggct ggaatcttcc atccagcaag gtcagcagca
    16021 gctaagtgaa ctgcgtgcca acgaatcccg tctgcgtaac agcattgccc gtgctgaagc
    16081 cgcggcgaaa gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc gcgagcgcca
    16141 gaaagaagcg acgcgcaaag gcaccaccta caaaccgacc gaaagcgaaa aatcgctgat
    16201 gtcccgaact ggtggcctgg gtgcgccgcg tggtcaggca ttctggccgg ttcgcgggcc
    16261 gacgctgcat cgctatggcg agcagctaca gggtgaatta cgctggaaag ggatggttat
    16321 cggtgcctct gaaggtactg aagttaaagc aattgccgac ggtcgagtga ttctggctga
    16381 ctggctgcaa ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg acatgagtct
    16441 ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg cgggccagcc
    16501 aattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt tcgaaattcg
    16561 ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt tgtttccatt
    16621 tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg tacttgctgg
    16681 caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa accaggtgct
    16741 ggcgatgcct tccgctatct ccgtcgctgt attacccgat tcaccgcacg ccagagagat
    16801 ggcgaccaaa gcgcataaca gcggtcacga agtgttgatt catctcccga tggcaccgtt
    16861 gagtaaacag ccgctggaga aaaatacgct acgcccggag atgagcagcg acgaaattga
    16921 gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca acaaccacat
    16981 gggcagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg cgctggagcg
    17041 ttacaatctt tacttcctcg acagcgtaac catcggtaat acccaggcga tgcgcgccgc
    17101 gcaaggtact ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt cgcagaacga
    17161 agcggacatc cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca acggttcaac
    17221 cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga tggtttataa
    17281 cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac cgcaggttga
    17341 tacttctaca ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt tccgtggcgt
    17401 gaagctgtgc aaaccgaaga aaccgataga acctgtttat gctaatcgct tctttgaagt
    17461 attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc agtggcaagg
    17521 ctggggcaaa cagcccgaag cggcgaagtt taacgctagc gcaaattaaa ggcgctacgc
    17581 gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa agaccacagt
    17641 aacgcctggt aaccgacctt cacgctgcgc acattggtaa tcaggcgctt atacataccc
    17701 ggaagtaaat atctcggcaa tcatccgttg gcgggtaaga atatccggct ctttgcg
//
LOCUS       DAZOZX010000079        17743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZOZX010000079 DAZOZX010000000
VERSION     DAZOZX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.079"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHUPT_002608"
     CDS             <1..194
                     /locus_tag="ACHUPT_002608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820137.1"
                     /translation="TCEDLSILSFLVFIFHFLFSSLSGSVLVLVFSFLFLFFLQGYAK
                     TPKHFSPQKHPIILPDQSP"
     gene            complement(265..1638)
                     /gene="eutA"
                     /locus_tag="ACHUPT_002609"
     CDS             complement(265..1638)
                     /gene="eutA"
                     /locus_tag="ACHUPT_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0820138.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(1613..2092)
                     /gene="eutP"
                     /locus_tag="ACHUPT_002610"
     CDS             complement(1613..2092)
                     /gene="eutP"
                     /locus_tag="ACHUPT_002610"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0820139.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(2105..2440)
                     /gene="eutS"
                     /locus_tag="ACHUPT_002611"
     CDS             complement(2105..2440)
                     /gene="eutS"
                     /locus_tag="ACHUPT_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0820140.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(2733..5012)
                     /gene="maeB"
                     /locus_tag="ACHUPT_002612"
     CDS             complement(2733..5012)
                     /gene="maeB"
                     /locus_tag="ACHUPT_002612"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0820141.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDSLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            5301..6251
                     /gene="tal"
                     /locus_tag="ACHUPT_002613"
     CDS             5301..6251
                     /gene="tal"
                     /locus_tag="ACHUPT_002613"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0820142.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            6271..8274
                     /gene="tkt"
                     /locus_tag="ACHUPT_002614"
     CDS             6271..8274
                     /gene="tkt"
                     /locus_tag="ACHUPT_002614"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0820143.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(8377..9420)
                     /locus_tag="ACHUPT_002615"
     CDS             complement(8377..9420)
                     /locus_tag="ACHUPT_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0820144.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(9546..10121)
                     /gene="nudK"
                     /locus_tag="ACHUPT_002616"
     CDS             complement(9546..10121)
                     /gene="nudK"
                     /locus_tag="ACHUPT_002616"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR0820145.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(10189..12168)
                     /gene="aegA"
                     /locus_tag="ACHUPT_002617"
     CDS             complement(10189..12168)
                     /gene="aegA"
                     /locus_tag="ACHUPT_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0820146.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            12374..14091
                     /gene="narQ"
                     /locus_tag="ACHUPT_002618"
                     /pseudo
     CDS             12374..14091
                     /gene="narQ"
                     /locus_tag="ACHUPT_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            14293..17406
                     /gene="acrD"
                     /locus_tag="ACHUPT_002619"
     CDS             14293..17406
                     /gene="acrD"
                     /locus_tag="ACHUPT_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0820147.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(17505..17564)
                     /gene="ypfM"
                     /locus_tag="ACHUPT_002620"
     CDS             complement(17505..17564)
                     /gene="ypfM"
                     /locus_tag="ACHUPT_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0820148.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4166 a         4735 c         4587 g         4255 t
ORIGIN      
        1 gaacatgcga agatttaagt attctttctt ttttggtttt tatttttcat tttctctttt
       61 cttctctttc cggttctgtt ctggttttgg ttttttcctt tttattttta ttctttcttc
      121 agggttatgc taaaacacca aaacattttt caccacaaaa gcatccaatc atcttgccag
      181 accaatcacc ttaaccggat acactgccgg atataatccc actaacaccc agacaccaag
      241 caccatacct gataactacc gtattcagga aggaaatgcg agtgatttca ccgtcaccgg
      301 cacaaccgat ccgccaaaaa gaggcgtacc aatgtcgata tagtcccccg cgcggacaat
      361 cacttcgtca atgactgcca acgggagttg ttgtagctgt gggcgcaaca acatgcccag
      421 agctttacca aagtcctgcc cggccaccac cagcagggga tgcggattcg gaaaacgcgc
      481 gacgaaatcg accagcgcgt tgatgaccgt cagaaccgcg gcgtaacgca caggcagcga
      541 ggcgggaagc gccagcacgt acacgtcagt tttgggatca agatccagct gaagcagcgc
      601 ctgttgccag gcactcacca gatccgtttc atcaatcggg atcgccaccg gcaaattgcg
      661 cagcggcagt tgtacgccct ccagccagat tgtgctgcca gagagcgaaa gggtatgtgc
      721 acccgcgcca atcaccgtgg agcgtacggt ttgcgccgga aactgcacat tcatctcacg
      781 caggcgcgga tggtcatgca gcgccgttgc cagaagtggg ccaatatcgg caaaacagaa
      841 cgggttggcg ggctggtggc gataacattc gcccacgcct ccagaaagcg taatgatttc
      901 gggcgtaaca cctgcgggca gcaaaccggt ttgcatcaat gcctgcgcga gcggcgaaag
      961 cgttccgtca atcacttcga caatcagcgc cgccatccgc cgggtaacct gcaccagctg
     1021 cgcgccggtc agcgaacggg cgtcagtgcc tgcaccgaag cactcatcca caatcatctg
     1081 ccccggttta tgagcgtaaa ccacgcgccc ctggctgtcg gtttccagca ggcgaccacc
     1141 gacgttgaga caggcagtgc cgctgatttt tccggcatcg aacagggcgt agttcgcggt
     1201 gccaccgccg atatcgatat tcagtacccg acacagccgt tgttcagaaa gggtttgcgc
     1261 cccggctccg tgaccggcga tcacggattc gaggtgcggc ccggcgctgg cgacgacaaa
     1321 atcgcccagc gactgttttt ccttcatcac tttttccatc agctgtgcga ccaggctttc
     1381 ggtaaactgg ccttccggca gctgggcaat gatggtttcg cggatgcgct ggctttcggt
     1441 tttgtcggct ggcacgctgg cgggcacgga cgccgttacc gggatcgatt catcacattc
     1501 ggtgatggta aaacccagta gatccgccac ttcacgggct tccggggtaa taatgctggc
     1561 gcgcagaacc acggacattg cctgttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc
     1621 tgtgatgagt tttttcacct gcttcctcct gtttggtgag agatgccaga taatccacca
     1681 gttgctgcac gctttgcggg tcgtggctgt ttagctcgaa aatcggctct tcgaaacccg
     1741 tctcaagcaa taactttcgc gttgcggcga cgtcagcatc tggcatgtcc gttttactga
     1801 tgacggcgat ttgccgtttg ctgacgccaa tatccagcaa cccggcaggc aaccgacttt
     1861 ccgggtcatt tgcgccatgg acatagatca gcatgtctac atcctgtagc gtggtaatta
     1921 aggcgtgata ccagcgggga tggctaaaat attcacccgg cgtatcaata tcgcccttat
     1981 cattaaattc cacggccagt gtttttctgg cgagggtata attcccctgc aacgcattaa
     2041 ataacgttgt ttttcctgcc ccgacgctac ccacaaacgc aattcgtttc atgaatgcct
     2101 cgtattaact tttggtcatt tcacaaagcg tataatttaa taagcgacct aaaccgctga
     2161 cggtttgtga taacgcttct tctactgcgc ccaccgagcc ataaatcacc aatgcaccgc
     2221 tgaagcggtc gagaaaaccg atatgcacat cagcggcttt cagcgctaaa tcaccggcga
     2281 tcatcgccgt ttcgccggga gttagcgtca taatgccaat tgcgcccgca tccggaacgc
     2341 cgattttctt cgccagttcc tcaccgggat gcgcgatgag atgcgccagt gtgacctgtt
     2401 tgccgggcac aaattcctga atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa
     2461 ctttcgtgta tcgtgacaat ttttcgtcag agtaaataac aaaattcggc aagcgcgttt
     2521 aaaaggtgag gtagatcaat aagaatggag agatatttac tgttatgaat aaactttcat
     2581 atgcgacgca tgaatataaa taaaataata tcgagatatt ttatatactg atttttttat
     2641 tattcgcaaa ctcagtaata agctttgcta atatatttca ggaaaaccgc ttaccctgaa
     2701 tattcagggt aagcgtgaga gttaagaatg aattacagcg gttgggtttg cgcttcaacc
     2761 acggccagcg ccaccatatt gacgatacga cgcaccgatg cgatcggcgt taacacgtga
     2821 accggtttcg ccacgcccat cagcaccgga ccgacagtca caccttccga gctggaaacg
     2881 cgcagtaagt tgtaactaat gcgggcagct tccatgttcg gcatcaccag aatattggca
     2941 gaacctttca aagggctgtc cggcatacgg tcgttgcgaa tcgcttccac cagcgctgca
     3001 tcgccgtgca tttcaccatc aatcatcaga tctggcgcac gatccctgac cagttccagc
     3061 gcctgacgca ttttgctcga cgacgggcag tcagaagaac caaagttgga gtgcgacaac
     3121 agagcaacgc gcggctcaat accaaaacga cggacagttt ctgccgccat caaggtgatc
     3181 tccgccagct cttctgcgtc tggttcgtca ttgacgtagg tatcggcaat aaaggtgtta
     3241 ccactcggca gcagcagcgc attcattgca cctgcggtgt gaacgccatc gcgataacca
     3301 aagacatttt tcaccacact aaaatgttca tgataatcgc ccaccgtacc gcagatcatt
     3361 gcatcggctt ccccacgctg aaccatgatc gcgccgatca ctgtcgggtt actgatcagc
     3421 gcacgctgcg cctgttcctg agtgacaccg cgacgcttca tgatctggaa gtattcggtc
     3481 cagtactctt taaagcgcgg atcggattcg ttattgacga tttcaaaatc aacgcccgct
     3541 ttgatctgca agcccagttt ctgaatgcgc atttcgatca cgttcggacg accgataagg
     3601 atcggtttcg ccagtcccag cgttaccagt tcctgagtcg catgcagaac gcgcgcctct
     3661 tccccttccg gcagaactac gcgcttcggc gctttgcgag cctgggagaa aatcggcttc
     3721 ataaacaggt tggttttgta aacgaactca gtcagcttgt cgatgtagac gtcgaaatca
     3781 gcaatcggac gcgtcgccac gcccgactcc atcgccgctt ttgcgaccgc aggagcgatc
     3841 ttaacgatca agcgcggatc aaacggtttt ggaatgatgt attccggacc aaagctcaga
     3901 tcctgaacgc catacgctga agccaccact tcactctgtt ctgcatgggc cagttctgca
     3961 atcgcgcgta ccgccgccag tttcatctct tcgttgatgg cggttgcgcc aacgtccagc
     4021 gcgccacgga agatgaacgg gaagcaaaga acgttgttca cctggttcgg gtagtcggaa
     4081 cggccggtac agatgatggc atccggacgc acttctttcg ccagcggcgg cagaatttcc
     4141 ggttccgggt tcgccagcgc caggatcatt ggtgcccgag ccattttctt caccatttcc
     4201 tgggtcagca ctttcgggcc ggaacagccc aggaaaatat ccgcaccttc aatcacatca
     4261 tcgagggtac gtttgccgtc atccaccacc gcatatgcgg ctttggtttc cgccatattt
     4321 ggctcacggc cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc
     4381 agccccagcg ctaccagcag gttcatacag gcgattgctg cggcacccgc gccggaaacc
     4441 accatccgca cgtcggagat gtttttctcc accacgcgca agccgttgag gatggcggca
     4501 gtgctgatga ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt catccgctcg
     4561 cgcagtttct gttcaatata gaaacattct ggcgctttaa tgtcttcgag gttgatgccg
     4621 ccgaaggtcg gttcgagcgc ggcgacgact tcaataaatt tgtccgggtc gagttcatca
     4681 acttcaatgt caaatacatc aatcccagcg aatttcttaa atagaacgcc cttgccttcc
     4741 atcaccggtt tgcctgccag cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta
     4801 gagatcaccg caaccaggtt accgcgcgcg gtatatttgt aggcttttag cgagtctttt
     4861 tcgatttcaa gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc
     4921 gttgccagag gcttggttgg agaaacctgg attttccctg gaactggaaa ttcatggaaa
     4981 tcaagtgcac tttgttttaa ctggtcatcc atttggttgt tcctttcacg taacgttcac
     5041 aaataaagtg tgtgggcaac agcccctgcc cacaacgtgg cgcacattat taccctgccg
     5101 gagtctacag actttgagca agtccaaact ctcaccatta atataatgtt ttggtaataa
     5161 tcctataaca ctgatgttac ctgcttaatc cagcaatacc atgcctgtct gctatgcttt
     5221 tttgatgcgt ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc taacacttta
     5281 cttttcaagg agtatttcct atgaacgagt tagacggcat caaacagttc accactgtcg
     5341 tggcagacag ctgcgatatt gagtccattc gccattatca tccccaggat gccaccacta
     5401 atccttcgct gttactcaag gctgccggat tatcacaata tgagcattta atagacgatg
     5461 ctatcgcctg gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg
     5521 acaaactggc ggtcaatttc ggtgctgaaa ttctcaaaat cgtacccggt tgcgtgtcaa
     5581 cagaagttga tgcacgcctc tcttttgata aagaaaagag tattgagaag gcgcgccatc
     5641 tggtggactt gtatcagcaa caaggcgttg agaaatcacg cattttgatc aaactggctt
     5701 cgacctggga aggaattcgc gcggccgaag agctggaaaa agaaggtatt aactgcaacc
     5761 tgacgctgct gttttctttt acccaggcgc gggcctgtgc ggaagcaggc gtttttctga
     5821 tttcgccgtt tgtagggcgt atttatgact ggtatcaggc acgcaagccg atggacccgt
     5881 atgtggtgga agaagatccg ggcgttaaat cggtgcgcaa tatctacgac tactataagc
     5941 aacaccacta tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg
     6001 ccttaaccgg ctgcgatcga ctgactatcg caccgaattt gctgaaggag ctgcaggaaa
     6061 aagtttctcc agtggtacgt aaattaatcc cgccttctca gacgttccca cgcccagcac
     6121 ccatgagcga agcggagttc cgttgggagc acaatcagga tgcgatggcg gtagaaaaac
     6181 tgtctgaagg cattcgtctg ttcgctgttg atcaacgcaa actggaagat cttcttgccg
     6241 ccaaactata aaccagccac ggagtgttat atgtcccgaa aagaccttgc caatgcgatt
     6301 cgcgcactca gtatggatgc tgtacaaaaa gccaattctg gtcatcccgg cgcgccgatg
     6361 ggcatggctg atattgccga agtgctgtgg aacgattttc ttaaacataa tcctaccgac
     6421 ccaacctggt atgatcgcga ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc
     6481 tacagtttgc tacatctgac cggttacgac ctgccgctgg aagaactgaa gaacttccgt
     6541 cagttgcatt cgaaaacccc aggccacccg gagattggct atacgccagg cgttgaaacc
     6601 accaccggcc cgcttggaca aggtttggcg aacgccgtcg ggctggcgat agcagagcgt
     6661 acactggcgg cgcagtttaa ccagccagac catgagatcg tcgatcactt cacctatgtg
     6721 tttatgggcg acggctgcct gatggaaggt atttcccacg aagtctgttc gctggcaggc
     6781 acgctgggac taggcaagct gattggtttt tacgatcaca acggtatttc catcgacggt
     6841 gaaacagaag gctggtttac cgacgatacg gcaaaacgtt ttgaagccta tcactggcat
     6901 gtgatccatg aaatcgacgg tcacgatccg caggcggtga aggaagcgat ccttgaagcg
     6961 caaagcgtga aagataagcc gtcgctgatt atctgccgta cggtgattgg ctttggttcg
     7021 ccgaataaag caggtaagga agaggcgcac ggcgcaccac tgggggaaga agaagtggcg
     7081 ctggcacggc aaaaactggg ctggcaccat ccgccatttg agatccctaa agagatttat
     7141 cacgcctggg atgcccgtga aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt
     7201 gccgcctata aaaaggctca tccgcaactg gcagaagagt ttccccgacg gatgagcggt
     7261 ggtttaccga aggactggga gaaaacgact cagaaatata tcaatgagtt acaggcaaat
     7321 ccggcgaaaa tcgctacccg taaggcttcg caaaatacgc ttaacgctta cgggccgatg
     7381 ctgcctgagt tgctcggcgg ttcggcggat ctggctccca gcaacctgac catctggaaa
     7441 ggttctgttt ctctgaagga agatccggcg ggcaactaca ttcactacgg ggtgcgtgaa
     7501 tttggcatga ccgctatcgc caacggcatc gcgcaccacg gcggctttgt gccgtatacc
     7561 gcgacgttcc tgatgtttgt tgaatacgcc cgtaacgccg cgcggatggc ggcactgatg
     7621 aaagcgcggc agattatggt ttatacccac gactcaattg gcctgggcga agatggtccg
     7681 acgcaccagg ctgttgagca actggccagc ctgcgcttaa cgccaaattt cagcacctgg
     7741 cgaccgtgcg atcagatgga agcggcggtg ggctggaagc tggcggttga gcgccacaac
     7801 ggaccgacgg cactgatcct ctcaaggcag aatctggccc aggtggaacg tacgccggat
     7861 caggttaaag agattgctcg tggcggttat gtgctgaaag acagcggcgg taagccagat
     7921 attattctga ttgccaccgg ttcagagatg gaaattaccc tgcaagcggc agagaaatta
     7981 gcaggagaag gtcgcaatgt acgcgtagtt tccctgccct cgaccgatat tttcgacgcc
     8041 caggatgagg aatatcggga gtcggtgttg ccttctaacg ttgcggctcg cgtggcggtg
     8101 gaagcaggta ttgccgatta ctggtacaag tatgttggtc tgaaaggggc aattgtcggg
     8161 atgacgggtt acggggaatc tgctccggcg gataagctgt tcccgttctt tggctttacc
     8221 gccgagaata ttgtggcaaa agcgcataag gtgctgggag tgaaaggtgc ctgatggtga
     8281 tcgccggatg ctgtatgttc ccctcaccct aaccctctcc ccagaagggc gaggggacgg
     8341 tccgtgcgct ttttagggcc gcatctgcga ttttgtttaa cgggtgatcc acaacgtggg
     8401 cctggcgtct ggcccatgcc agttatcgca ggtaggttct gcggcgtaac gcaccaggcg
     8461 aaaacgctga ccgtcaaagc gccagcgcgc ctgaatgcca caatcgctta atccgcgccc
     8521 tttcgctaag gtcaccagtt cacgcgattt ctcatcaaat gttgcgttca tcagttccag
     8581 ttcattcgtc tcctggccgt tgttgaacgg caaacgcaac cgaaccgggc gcgaggctag
     8641 cggcttttta cgcgacacaa tccatgccaa atcaatggtg ttataggccc ctgcctcaca
     8701 gctaatcatc atcagtgctt tatcatcggt cagcgcagtg acattcacct cacgacgtaa
     8761 cggatcaagc gagcagcgca gaccattcat ccgccagttt ccataatcca gcaaatcgtt
     8821 acgttcttcg agtgagagtg gcgtcggcgt tgggttaacc accgcgacct ctttcagcgc
     8881 aggcgcgggc ggtacgctga gaggcggttc gtcccctttc ttgatccacg cggtttcact
     8941 gccaacgcgc ttttgctgag catcaataaa caacaacgcc gcttttaagc cgctcagaga
     9001 aatggtctga ttgccgtcgc gtaaggtgat cgccttccct tcctgaatta tctgcaaaaa
     9061 cgcggtgatg gttgccgtat catcggttac taataaccat ggtgaaatcc gccacttgtc
     9121 accacttaat gccaacggct caccatctaa tagcagccgt ggcgctatct ccccttcaga
     9181 cgcatccggc gacttcaacc caccgcgctc aatacgtaaa acggcatcgg tatgcgcccc
     9241 agcgctgcgg ctcagggtca tcaccagtcc attatgatcg cccgtattgc gcgccacaca
     9301 aaaattttga ttattacagg tgacctgcca gtcggaaaac gcccgttgcg ctggtgccgc
     9361 ccacaccaaa gacgtcggca acaaagcgaa aaaaaagaga aggaaaatgc gatagcgcat
     9421 ggacggtacg accccagaag ttcaaacaaa cagcacaagt cgtatcttcg ttgcgcaggt
     9481 agcgcggctc aatcggattt gtcggattga gccaataata aacaacgaaa tattattgct
     9541 atttttcagt ccattaaatg tgacatttgc aaatagttaa gcaataacac cgtcttaccg
     9601 tcacgtatct cgccggtttt gatcatctcc agcgcctggc tgaacggcag ctcgagcact
     9661 tcaatatctt catcttcaac accgccaccg gcgttagcgc gctgattgtc actgtattcg
     9721 gcgataaaaa agtggattag ctcagtcaca ccgccaggcg acatatacag ttcaaataat
     9781 ttgcgcactt cgcccacttc atagcccgtc tcttcaatcg cttctttacg aatgcacact
     9841 tccggttcgt cgttatccag cagcccggcg caggtttcaa tcagctgccc gctttcattg
     9901 ccattaaccc aggtagcgac acggaactga cgaatcagaa ccacgctctt tttcttcgcg
     9961 ttgtacagga ggatcgtcgc accattaccg cgatcgtaaa cttcacgttt atggcggata
    10021 acttcgccat ctttgcgcgt gagatcgtaa gtaatgttgt gcagggtgaa atagttatcg
    10081 gagagaattt tgtctttaat gagggtgatt tgttgcgtca taccgactcc acagcgcgaa
    10141 atgaacaatt atattacgct gtgaagtcgg gtttgtctgc gcaggctatc agtgagattt
    10201 gactgatttt acccccagcc agtcaataat cccctgtgcc gcatgacgtc cttctgccat
    10261 tgcggtaacc accagatccg caccacgcac ggcgtcgcca ccggcgaaga ttttcggatt
    10321 ggtggtctgg taacggtact ggctttccac atccgcgatg atgcggcccc atttgtctac
    10381 cgttacaccg tgcgactcca gccacggcat cccgtgtgga ttgaagccaa acgccataat
    10441 caccgcatcg gctggcatga caaattcact gccctcaatc ggtacaggtc gccgacgccc
    10501 ctgggcatcc ggctcgccaa gacgcgtgcg caggaaacga attccgcaaa cgtgaccttg
    10561 ctcattcagc tcaagcgcca ccggctggac gttaaattcg aagttggccc cctcttcgcg
    10621 ggcgttcttc acctctttct tcgagcctgg catgttagct tcatcacgac gataagcgca
    10681 ggtgacgtta ctcgcgccgt ggcgcagcgc agtacgcaca cagtccatcg cggtgtcgcc
    10741 gccgcccagt accacgacgt taagtccggc ggtattgata aacggctctt ccggtagctc
    10801 ttcgagcccc atcacctgtt tagtgttggc aatgaggaac ggcagcgcgt cataaacgcc
    10861 cggcgcatct tcattgggta aacccgcttt catggaacgg taagtgccta cgccaacgaa
    10921 gaccgcgtcg tattgttcca aaagcaaatc caaagagaca tctttaccca cttcacaatt
    10981 gagttcgaag tgaatcccca tcgcgctgaa gatttcccga cggcgtgcca gcagggattt
    11041 atccagtttg aaagaaggaa tgccgaaagt gagcaagcca ccgatttctg gatggcgatc
    11101 gtacaccgtc accgccacgc cattgcgggt cagaacatcc gcacaggcca gccctgccgg
    11161 acctgccccg ataatcgcca cccgcttgtc cactttggtg acatgactta agtcaggacg
    11221 ccaacctttc gccaacgcct gatctgaaat atagcgttca atgttgccga tagttaccgc
    11281 gccgtgctca tcgcgaatag tacaggcacc ttcacacaaa cggtcttgcg gacaaacgcg
    11341 tccggtaatt tccggcaggg tgttggtctg gtgagaaagc tcgacggcgg cgtcgatgtt
    11401 tccggctttc accagttcaa tccactgcgg aatatggtta tgcagcgggc aggtccattc
    11461 acaaacgctg tgctcgccga acttaaggca gcgcgaggct tcccgttgtg cctggtcggc
    11521 gcgaaatggc agataaattt catcaaaacc ggttttgcgc gcttcaatcg ccagtttatc
    11581 cggctcgcca cgcgcgggcg ttgcctgcat ttgttcgact ttactcatta ccggcatttc
    11641 ttgtgcagcg gtactggcat gccacggttg atgctcctga cgtgcggtgc gcaagcggcg
    11701 ggatttcgcc atgccggaga gtgcgacgtc agtgaccagt tgcagcgcgt ccgccgggca
    11761 attctcgaca caggcaggac cgttttcgcg ccctgcacaa aggtcgcatt tatgcgccgt
    11821 ggcttttact tttcctgccg cgacgggtgt caggacgatt tgcatcgtac caaacggaca
    11881 ggccaccacg caggatttac agccaataca cttttgctga ttgacctgaa tgctgtcatc
    11941 aacgtggctg attgcgccat tagggcagct acgggcgcag ggcgcatctt cacaatggtg
    12001 acaggtcact gcactacgtt gctgctgatg tttgataacc gtaattcggg gatgaaaatg
    12061 gtgttggctc aggacatgtt gctcatcatt gtgagccatg acacaggcga tttcacaagc
    12121 atgacaaccc agacattgct gactgttggc cataataaaa cgattcataa cgaccttctt
    12181 ttttggttgt aaaaacctta ttctttatat gagtgttgtt attacccgac ttacagggga
    12241 accggcaatg ttcatgtgcc cagaataagt aactatttcg ctataaactg tggcagatca
    12301 aataatccca tcactgacta aattgcgttt cagcgaactg gaacattaat gattttttgt
    12361 ggagaagacg cgtgtgattg ttaaacgacc cgtctcggcc agtctggccc gggccttttt
    12421 ttacattgtg ctgctgtcga ttctttccac gggtatcgct ctgctaactc tggcgagcag
    12481 tttgcgcgac gctgaggcta tcaatattgc cggatcgctg cgtatgcaga gttaccgcct
    12541 gggctacgac ttgcaaagtg gcagtccaca actcaatgca catcgccagt tgtttcagca
    12601 ggcactgcat tcaccggtat taaccaacct caacgtctgg tatgtgccag aagcagtaaa
    12661 aactcgctat gcgcatctga atgccaactg gctggagatg aataatcggc tcagcaaggg
    12721 cgatttgccg tggtatcagg ccaatattaa taattatgtt aatcagatag acctgttcgt
    12781 gctggcttta cagcactacg ctgaacgcaa aatgctgctg gtggtggcga tttccctggc
    12841 tggcggtatc ggtattttca cgctggtctt ttttactttg cgccgcatac gccatcaggt
    12901 ggttgccccg ctgaatcagc tggttaccgc cagtcagcgt attgaacacg ggcagttcga
    12961 ctcgccgccg ctggatacca gtctgccgaa tgagcttggt ctgcttgcaa aaacctttaa
    13021 ccaaatgtcg agcgagctgc ataaattgta cctttcgctg gaggcgtcag tagaagaaaa
    13081 gacccgcgat ctccacgaga ccaagcgtcg tctggaggtg ttgtatcagt gcagtgttcc
    13141 caggcgctga acaccagcca gattgatgtg cattgtttcc gccatatttt gcagattgtt
    13201 cgcgacaatg aagcagctga atatctggag ctgaacgttg gggataactg gcggattagc
    13261 gaagggaagc caaaccctga gctgccaatg cagattttac cggtgacaat gcaagagacg
    13321 gtttacggcg aactgcactg gcaaaatagt cacgtttcat catcagaacc gctgcttaac
    13381 agtgtttcat cgatgctggg acgcggtttg tactttaatc aggcgcagaa gcattttcag
    13441 cagttattgt tgatggaaga acgtgcgacc atcgcccgcg aattgcacga ctcgctggct
    13501 caggtgcttt cttacttacg tatccagttg acgttactga agcgttcgat accggaagat
    13561 aacgccaccg cacaaagtat catggccgat ttttcccagg cgttgaatga tgcttatcgg
    13621 cagttacgcg agctgttgac tactttccgc ctgacgctgc agcaggcgga tctcccctcc
    13681 gcgttgaggg aaatgctgga tacgttacaa aatcaaacca gcgctaaact gaccctcgac
    13741 tgccgtctgc caaccctggc actggatgcg caaatgcagg tgcatttgtt gcaaattatt
    13801 cgcgaagcgg tgctgaatgc gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc
    13861 gtcaccgcgc cggacggcaa tcacacggtt tatatccgtg ataacgggat tggtatcggt
    13921 gaaccgaaag aacccgaagg tcattatgga ctgaatatca tgcgcgaacg cgcggaacgg
    13981 ctgggcggga cgctgacttt ttcacaacct tccggcggcg gcacgttagt gagtattagc
    14041 tttcgctccg cagagggtga ggggaagggg aacacgccgg ctgaggtgta aataattacc
    14101 ctcacaccgg ccctctccct ttaaaggcga gagggaaaaa cgtgcccata ttgacctgta
    14161 agcgagtgtg tcagcaatct catgataatt tacattaact cctttttttc tccacgattg
    14221 gctcgtacct tgccgctaca gtgaagcaag tcaagcctac aacaatacgc agaaacacga
    14281 ggtcctcttt taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctgaca
    14341 atcctgttgt gtctgacagg taccctggcg attttttcat tgcccgttga acaatacccc
    14401 gatctcgcgc caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg
    14461 ctggaaaaca ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctgatg
    14521 tatatgtcat ctcagagcag tggcaccggt caggcatctg tcactttaag ttttaaagca
    14581 ggcaccgatc cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga
    14641 aagttaccgc aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaac
    14701 attctgacca ttgccttcgt ctctaccgat ggctcgatgg ataaacagga tattgctgat
    14761 tatgttgcca gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat
    14821 gcctatggtt cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc
    14881 cagatgacgg ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt
    14941 gggcaacttg gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc
    15001 cagtcactgc tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaggac
    15061 ggctcagagg taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat
    15121 tatcttagcc gcttcaatgg taagccagcc tccgggttgg gggtaaaact ggcctccagc
    15181 gcaaacgaaa tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtat
    15241 ttcccgcacg gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc
    15301 attgaagacg tggtgaaaac gctgctggaa gctatcgctc tggttttcct cgttatgtat
    15361 ctgttcctgc aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg
    15421 atgggaacct tctccgtact ttacgccttc ggttacagcg tcaacacctt aaccatgttc
    15481 gcgatggtgc tggcgatcgg tctgctggtg gatgacgcca tcgtggtggt ggaaaacgtc
    15541 gaacgtatta tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg
    15601 cagatccagg gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtaccaatg
    15661 gccttcttcg gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg
    15721 gcgatggtgc tgtcagtact ggtagcgatg ctcctcactc cggctctgtg tgccacgcta
    15781 cttaagccac tgaaaaaagg tgagcatcac gggcaaaaag gtttttttgc ctggtttaac
    15841 cagatgttta accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgt
    15901 agcctgcgct ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg
    15961 cgtttgccga cgtcgttctt accgctggaa gaccggggca tgtttactac ctcggtacaa
    16021 ttgcccagcg gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat
    16081 tacttcaccc atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg ttctggccct
    16141 gggggtaacg ggcaaaacgt ggcgagaatg tttatccgcc tgaaagactg gagcgaacgc
    16201 gacagtaaga ccggcacctc gtttgccatt atcgagcgtg caacgaaggc gtttaaccaa
    16261 attaaagaag ctcgcgttat cgccagcagc ccaccagcaa ttagcggtct tggtagttct
    16321 gcaggttttg atatggagtt gcaggaccac gctggagcgg gtcacgatgc gctgatggca
    16381 gcacgtaatc agttgctggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat
    16441 aacggcctcg atgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg
    16501 ctgggcgttg atatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat
    16561 gtgaatgact ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg
    16621 tatcgcatgc tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg
    16681 gtgcccttct ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg tctggaacgc
    16741 tataacggtt attctgcggt tgagattgtt ggggaagccg caccgggggt cagtaccggt
    16801 acggcgatgg atattatgga atcgttagtg aagcagctgc caaacggctt tggtctggag
    16861 tggacggcga tgtcgtatca ggagcggctt tccggcgcgc aggctccggc gctgtacgcc
    16921 atttccttgc tggtggtatt cctgtgtctg gcggcattgt atgaaagctg gtcggtaccg
    16981 ttctcggtaa tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc aacctggatg
    17041 cgcgggctgg aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg
    17101 gcgaaaaacg ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccatgac
    17161 ctgtttgaag cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacctcg
    17221 ctggcattta tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt
    17281 cagcatgcgg tgggtactgg cgtaatgggc gggatgattt cagctactat tctggctatt
    17341 tacttcgtgc cgctgttctt tgtgctggtg cgccgccgct tcccgctgaa gccgcgcccg
    17401 gaataagcaa taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt tccgattaag
    17461 aacctgctca gcgggttctt gctgtttata ctttgtctca ggaattactt acgaagcata
    17521 acttcgataa agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata
    17581 attatgggta ttctacggaa acaatatacc gtggtgaagc taatttactc gattgctgcg
    17641 atgactacct ccgggggaca aaccttatgt aaatactatg gtcctgcggt gataatttgt
    17701 atgtgataca cagcaacatt tcgagatatt catacggcat cta
//
LOCUS       DAZOZX010000080        17711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZOZX010000080 DAZOZX010000000
VERSION     DAZOZX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.080"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            66..704
                     /gene="ompW"
                     /locus_tag="ACHUPT_002621"
     CDS             66..704
                     /gene="ompW"
                     /locus_tag="ACHUPT_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0820149.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(764..1270)
                     /locus_tag="ACHUPT_002622"
     CDS             complement(764..1270)
                     /locus_tag="ACHUPT_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR0820150.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1316..1816)
                     /locus_tag="ACHUPT_002623"
     CDS             complement(1316..1816)
                     /locus_tag="ACHUPT_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR0820151.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(1902..2081)
                     /locus_tag="ACHUPT_002624"
     CDS             complement(1902..2081)
                     /locus_tag="ACHUPT_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0820152.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(2461..3267)
                     /gene="trpA"
                     /locus_tag="ACHUPT_002625"
     CDS             complement(2461..3267)
                     /gene="trpA"
                     /locus_tag="ACHUPT_002625"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0820153.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(3267..4460)
                     /gene="trpB"
                     /locus_tag="ACHUPT_002626"
     CDS             complement(3267..4460)
                     /gene="trpB"
                     /locus_tag="ACHUPT_002626"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0820154.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(4472..5833)
                     /gene="trpCF"
                     /locus_tag="ACHUPT_002627"
     CDS             complement(4472..5833)
                     /gene="trpCF"
                     /locus_tag="ACHUPT_002627"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0820155.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(5834..7429)
                     /gene="trpD"
                     /locus_tag="ACHUPT_002628"
     CDS             complement(5834..7429)
                     /gene="trpD"
                     /locus_tag="ACHUPT_002628"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0820156.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(7429..8991)
                     /gene="trpE"
                     /locus_tag="ACHUPT_002629"
     CDS             complement(7429..8991)
                     /gene="trpE"
                     /locus_tag="ACHUPT_002629"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0820157.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(9013..9106)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9083..9127)
                     /gene="trpL"
                     /locus_tag="ACHUPT_002630"
     CDS             complement(9083..9127)
                     /gene="trpL"
                     /locus_tag="ACHUPT_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0820158.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            9265..10146
                     /gene="rnm"
                     /locus_tag="ACHUPT_002631"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             9265..10146
                     /gene="rnm"
                     /locus_tag="ACHUPT_002631"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0820159.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10143..10763
                     /locus_tag="ACHUPT_002632"
     CDS             10143..10763
                     /locus_tag="ACHUPT_002632"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0820160.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10864..11736
                     /gene="rluB"
                     /locus_tag="ACHUPT_002633"
     CDS             10864..11736
                     /gene="rluB"
                     /locus_tag="ACHUPT_002633"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0820161.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(11776..12366)
                     /gene="cobO"
                     /locus_tag="ACHUPT_002634"
     CDS             complement(11776..12366)
                     /gene="cobO"
                     /locus_tag="ACHUPT_002634"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR0820162.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(12363..13121)
                     /locus_tag="ACHUPT_002635"
     CDS             complement(12363..13121)
                     /locus_tag="ACHUPT_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0820163.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13341..14390
                     /gene="sohB"
                     /locus_tag="ACHUPT_002636"
     CDS             13341..14390
                     /gene="sohB"
                     /locus_tag="ACHUPT_002636"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0820164.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14426..14677)
                     /locus_tag="ACHUPT_002637"
     CDS             complement(14426..14677)
                     /locus_tag="ACHUPT_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0820165.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            15057..17654
                     /gene="topA"
                     /locus_tag="ACHUPT_002638"
     CDS             15057..17654
                     /gene="topA"
                     /locus_tag="ACHUPT_002638"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0820166.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         4316 a         4414 c         4640 g         4341 t
ORIGIN      
        1 gatcatttgt tactccaatg taggtatatt cgtcacgttt ttataaccat aacgacggag
       61 cggatatgaa aaagttaaca gtggcggctt tggcagtaac aactcttctt tctggcagtg
      121 cctttgcgca tgaagcaggc gaatttttta tgcgtgcagg ttctgcaacc gtacgtccaa
      181 cagaaggtgc tggtggtacg ttaggaagtc tgggtggatt cagcgtgacc aataacactc
      241 aactgggctt gacgtttact tatatggcga ccgacaacat tggtgtggaa ttactggcag
      301 cgacgccgtt ccgccataaa atcggcaccc gggcgaccgg cgatattgca accgttcacc
      361 atctgccgcc aacactgatg gcgcagtggt attttggtga tgccagcagc aaattccgtc
      421 cttacgttgg ggccggtatt aactacacca ccttctttga taatggattt aacgatcatg
      481 gcaaagaggc ggggctttcc gatctcagtc tgaaagattc ctggggagct gccgggcagg
      541 tgggggttga ttatctgatt aaccgtgact ggttggttaa catgtcagtg tggtacatgg
      601 atatcgatac caccgccaat tataagctag gcggtgcaca gcaacacgat agcgtacgcc
      661 tcgatccgtg ggtgtttatg ttctcagcag gatatcgttt ttaattccgc acaaaaacga
      721 ccccgtaata tacggggtca ataaggacat ggtataaagg gtattatttc ttcgcttcta
      781 cgccatcagt ttcagaacga attaaaaatt tctcagttgt ttgcggaata tgctgaatca
      841 gccagtcggc catttgcttt tcctcattta aaatcgcttc gatgatcgga attgagactg
      901 tatcaccggc attttttgct gctgctaata gtgaggtgta acaggtgatt tcaaattgct
      961 cgaagacata tccgccaata gagcctttaa ctatttcatc agaagggaat ataccaccga
     1021 ttgactgccc aagcgcagcc attttactca tggaatcttt aatgactgaa cgtgaaatgt
     1081 cattacgatc aagaatagtt tccagttgaa caatctggtt tttggtttca ctaagatgtt
     1141 gttcaatacg agcgcgtagt tcaggataat tatctatacg gctggccatg gattcaagca
     1201 tagattcggc ttgcttttcc attgcgtggg cgtcacgtaa ccagtcatga taatgttcaa
     1261 tacgattcat gaaaatgtcc tctttataaa atgactaaaa gttaaaattc atatttcagg
     1321 ctttattttc ggctttctta tttacgttat taagggccag atcagtcagt ttgatgtcgg
     1381 tggccttttc ttcttccagg gtttctttca gaagcttcgc tgctttacgg taacctaatt
     1441 gttcagccag cgtcgctaat gtcccgtaac tggcaatctc ataatgctcg actttctgtg
     1501 ctgcggcaat cagtgcggca tcacgcactt cgtttttctc ggtactttcg atgacctcat
     1561 tagcttcttc aataagacct tccattgcca cacatttcat gcgcttaatt ttcagatttg
     1621 attctgattc cacaacttga tcaattcgtt caatctgtcc atgagtttcc tcgaggtgcg
     1681 catgaaaagc ctgacttaat ttttcatttg atgttgctct tgcgagtttt gccagtgccc
     1741 gggttaattg tttttctgcg ctgtaggtat ctgaaagcag ttgaataaat acatcttcaa
     1801 tggtcttcat attcatattt ttctccagtg aaatcactgc gagccatacg gcccgcagat
     1861 gcaattatct ttatcagctg atattaataa ttaacggtga atcaggattt gcctgattta
     1921 ttaccaccgc tttgttgacc gcctttttta cccgcttcag atgcgcgttg cggatcattt
     1981 ttaaaattac cgccgctatg ctgaccgcct ttacggcctg cgtcggatgc cttctcacgg
     2041 tcttcggcga aatttcctga actaccacga tgttcggcca tgttatttct cccgttgcgt
     2101 tgcattgttt cattaatatg agtgttgtgt gtcgacactc attaaaatta gtcactaatg
     2161 agaattagtc aaattaagcg caacgagaag atagagggaa atatattttg aggaacattc
     2221 tggatatatt aacaattacc tgaggaatat gtgacttaga gaaaattatt gattttactg
     2281 gtgttatgtt gcgggattaa tttgtttgat tatattactg ttgggcggaa aaatgacgta
     2341 agttgacgtt cgaccggggt aagcgaaacg gtaaaaagat aaatattaaa tgaatttagg
     2401 atttttccgg cttcattaaa gaaagttaaa atgccgccag cggaactggc ggctgtggga
     2461 ttaactgcgc gtcgccgctt tcatcggttg tacaaaaact ttcagtgccg ccagcatttt
     2521 ctctggctca ttaatatgtt gctcgatgat tttaacaatg gccgaaccag aaatcgcgcc
     2581 cgcagctcct gcatcaatcg ctgcttttac ctgatccggg gcggaaatac caaacccctg
     2641 caatggaggt gcagcgttgt actctttcag cttcgcaacc agatgattga ggggtaacgc
     2701 ggcgcggttt tctgcgccgg tcacgcccgc tcgtgacagc aaatatgtgt aaccacgacc
     2761 gtaagaggct atctggcgca gcaggtcgtc atcggcattt ggcgggcaga tgaagatagg
     2821 tgcgacatta tggcgcaacg cggcctggcg gaagggcgcg gactcttcaa ctggcacatc
     2881 ggcaaccagc accgaatcga cgccgacttt ctcgcactgg gcataaaact catcaatgcc
     2941 tttgttaaac accagattgg catacatcaa caggccaatg ggaatggtcg ggtgtttctg
     3001 gcgaatcagt gccagcattt caaaacattg tgccggagtc acacctgccg caaaggcgcg
     3061 cagagtggcg ttttgaatgg tcgggccatc cgccagtggg tcggagaagg ggatacctaa
     3121 ctccagcgcg tcagcaccgg cttcaattag cgtatcgata attttcaatg actgctcaat
     3181 gcccggatca ccgagcgtga cgaaaggaac gaatgcgcct tctttgcgct ccttcaactg
     3241 ggcaaacaga gattcgtagc gttccatcag atttcccctc gtgctttcaa aatatcgtga
     3301 acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag ctgctcttta
     3361 tccgggtttt cgcgcatcat tttcagcgca tgggccaggg cgtgggagga ttccagcgcc
     3421 gggatgatcc cttcgtgcag gcacagcgtt ttgaaggctt caagggcttc atcatcggta
     3481 atagacacgt aatcagcgcg tccagtgctg ttaagatacg cgtgttgtgg accgacagac
     3541 gggaaatcca gtccggcgga gatggagtaa gattcttcaa tctgcccgtc ttcggtttgc
     3601 atcatcggcg ctttcatacc gaaataaatg cccacgcgac catgttttag cggtgcgccg
     3661 tgctcgccag tttcgatacc gtgaccacca ggctccacac caatcaggcc gacgttggtt
     3721 tcattgatga aatcagcaaa catgccgatg gcattcgaac cgccgccaac acaggcgata
     3781 acggcatccg gcaggcgacc ttctctttcc agaatctgcg ctttggtttc ttcgccaatc
     3841 atccgctgaa actcacggac aatggtcgga taaggatgcg ggccagccgc ggtgcccagc
     3901 atatagtgcg cggtttcata actgccggac cagtcgcgta gcgcctcatt acaggcatct
     3961 ttcagcgtgg cggaaccgct atgtaccggg atcacttccg cacccattaa gcgcatacga
     4021 aaaacgttag gcgactggcg ttcaacgtct ttggcaccca tataaatacg gcatttcagg
     4081 ccgagcaggg cgctggcaag ggccgacgcc acgccatgct gaccggcccc ggtttcggcg
     4141 atgatttcgg ttttacccat ccgcttcgcc agcaacgcct gccccagcac ctggttggtt
     4201 ttatgtgcgc cgccgtgcag caaatcttca cgcttgagat acagcgtggt gttcgtcccg
     4261 gctgtaatgt tctggcattt ggtcagtgcg gttggacgcc cggcatagtt tttcagcagg
     4321 tcgttgaact gggcttgaaa ttcaggatct ttttgcgcgc tgacaaaagc ttcttccagc
     4381 tggcgcagag caggcatcag gatttgtggc acgtacatgc cgccaaactc accaaaatag
     4441 gggttaagta atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc tggaaaaccg
     4501 aggccaaaag acgtgcgtct ttgatgcccg gttgcgactc tacagcagaa ttaaaatcaa
     4561 gtccggcgca gccggtttgt gccgcttcca cgcagttatc tgcgcctaag ccccccgcca
     4621 gcagaacgtt gccaagcgat tgaccattta atagtgacca gtcaaaacgt tgcccgcttc
     4681 caccctggcc gttgtctaaa acatatttat caacgtgctg aaactcgcgg gcgggcaggg
     4741 tttcaccgac gcttaatgct ttccagatgg caacatgtgc tggcagagct tcacgcagcg
     4801 tatcgatata cagctgatct tcattgccat gcagttgcac tgccgccagc gataacacct
     4861 tagctttgtc caccacatcg gcaatatcgt gattgcggaa cacgccaaca tactgcaacg
     4921 gtgccgcagc catcacttcc tgcgcctgtt caacgttgac gcaacgcggt gatgtcgcaa
     4981 caaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc tgctttagca tcttgcccac
     5041 gcgtcaggcc acatacttta ttctcaccca gcaacacccg gcgcacggcg gcgtgcaaat
     5101 cgtcatgggc catcaacgcc gaaccaatca gaaaaccgtt agcgaagtgg cttaactcgc
     5161 gcacctgagc gtaagtattg atgccggatt cgctgattac cgttacgttg tgccccagtt
     5221 tcggcgcaag ctcgcgggta cggttgagat caatcgacaa atcacgcaga tcgcggttgt
     5281 tgatgccaac gacctttgcc cctaatgcaa tggcgcgctc cagttcctct tcattactga
     5341 cttcggtcag cacccccatc tccagactgt gagcgacggc tgcaagctgg cgatattggt
     5401 cgtcatccag tactgaaagc attaataagc aggcatcggc ctggtaatag cgcgccagat
     5461 agatctggta agggtcgata atgaagtctt tacataaaat cggctgcggg gcgatttggc
     5521 tgacgatgag gaggaaatca aagctcccct gaaaatattt ctcatcagtc agcaccgaaa
     5581 ttgccgaagc gtaatgttta taaatggcgg caatgcgtgc cggatcgaaa tcatcacgga
     5641 tcacgccttt tgacggcgac gcttttttac actccagaat aaacgccgtg cgtgcaccct
     5701 gtagcgcatc ataaaaatgt cgtgtgctcg gctgaacctc attctgaaaa ctggccagcg
     5761 gttgctgctg tttgcgggtt tctacccaaa tcgccttgtc tgcgacgatt ttcgctaaaa
     5821 cggtttgcat catttaccct cgtgccgcca gcgcggtaac tctgtcgtaa gcggaaccac
     5881 tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag atcttcatgg ccatgcaggc
     5941 gcattaacat ggcgacgttc gcagcgacgg ctgcttcatg ggcggcgtcg cctttacctt
     6001 gtaacaagcg tgttaaaatg tcacggtttt cttcaggtgt tccgcctgcc agttgctcct
     6061 ggtggtaggg agtcaggcca aaatcttcag cggtcaattg atagctctta attttgccgt
     6121 catgcagctc ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc ccgccgctgt
     6181 gcaccaccgc cgcgcgttga taccccagca cgcgcaaggt ttcggcaatc ggcagcacca
     6241 gttccggact ataaacacca attaacgcca gcggcggatg cgccgggtta atcaatggcc
     6301 ccagcacatt gaacagggtg cgggttttca gttgctggcg aaccggcatc gcgtggcgga
     6361 atccggtgtg atatttcggc gcaaagagga aacatacgcc taactcgtcc agcgcctggc
     6421 gcgatttatt ggcgttcata tcaagattaa taccgaatgc cgccagcaga tccgacgagc
     6481 cggatttact ggagacgctg cggttaccgt gtttcgccac tttcagccca caggccgcgg
     6541 cgacaaacgc actggcggta gaaatattga tactgttgct gccgtcaccg ccagtaccga
     6601 cgatatcagc aaacagataa tccgggcgcg ggaacggcgc tgcgttttcc agtagcgcgg
     6661 ttgctgcccc ggcgatctcg ttcgggtgct caccgcgaat tttcatgctc accagcgccg
     6721 ccgccagttg ttccggcttc agctcgccac gcaccaccgc tgaaaacagc tggtggcttt
     6781 cttgttggct aagcgtctgc gcctgataca gtttttccag aatcggttgc agcgtgttgg
     6841 ctggctctag tttctgctgc gcccaggcca gcgtttgttc cagcaggcga gcgccctggg
     6901 tggtgagaat ggattccgga tggaactgga atccacaaac gcgatccgca tcgtgacgta
     6961 ctgccatcac catgccatta aaatgggcgt tgatggttaa accggccgga atgttactgc
     7021 caaccagcga gtgataacgc gccaccggca gcgggtttgt taatccggca aacatcgcct
     7081 gaccgtcatg ttcaatgctc gacgctttac cgtgaagaat ttcgcccgcc tgaccgacat
     7141 agcccccgta agcttcgaca attgcctgat gtccgaggca aatgccaata atgggcagct
     7201 tgccacgcaa gcgggtgagg agttccggca tacaaccggc ttcgctcggc acaccggggc
     7261 caggagaaag catcagtacc ggattgctca tcgtcgccag gcgttcaatt aaggtctgcg
     7321 ccggaatatg gttgcggtaa atcaccacgt tatgaccatt gctgcgcaac tgatctgcca
     7381 ggttgtacgt aaaagagtcg atattatcga gcagcagaat gtcagccatc agaaagtctc
     7441 ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg cgggctttgt tacgggtttc
     7501 gtcggcttcc gactgcggaa cagaatcaag gactacacca gcacccgctt gcacggtggc
     7561 gataccgttt tccaccagcg ccgagcggat cacaatgcag gtgtcgagat cgccatgcgc
     7621 ggtgaaataa cctaccgcgc cgccgtagct gccgcggcga cgaccttccg cctcggcaat
     7681 taactgcata gcgcgtactt tcggcgcacc gcttaacgtc cccatattca tacaggcgcg
     7741 ataagcgtgc agggcgtcaa gatcgtgacg cagttcgccg actacgcgag agacgaggtg
     7801 catcacatag gaataacggt caactttggt gagatcggcg atgtagcggc tgccaggggt
     7861 gcaaatgcgt gccagatcat tacgggcgag atcaaccagc atcagatgtt cagacagctc
     7921 tttatggtca gtacgcattt ccagttcaat acggctgtcg agatctctgt ccagtgaacc
     7981 atcggcgcga cgaccgcgtg ggcgtgttcc ggcaatcggg tagatctcaa tctggcggct
     8041 ggtggcatca tacttgagcg agctttccgg cgacgcgcca aatagggtga aatcattatc
     8101 ctgcataaaa aacatgtacg ggctgggatt actctttttc agcacgtaat aggccgccag
     8161 cggtgacggg cagggcagag agaaacggcg agatggcacc acctggaaaa tttctccagc
     8221 gcgaatcgct ttttgcaaca aacgcactac gccaccgaac tcttcatcgc tctgattaca
     8281 ttcacaacgc atatgcggca cggaaaccac tggcagcggc ggcgcggctt cggtcagttg
     8341 ctgacgtagt tcgttcaggc gagcagtgag acgttgtttt tcttcttcat tcggagcaaa
     8401 caggctggcc tgaatacggg tgcttttttt ctgatggtca atcaccatca gcgtttcagc
     8461 gagataaaaa cagaaatcag ggcagttatt ttccgctgac agttgcggta aatcttcaaa
     8521 tcccgccaca aggtcataag agaacaggcc gccgaagaac attgcttctc gttcttcctt
     8581 cggtacattc aacagattct gcaataaacg gaaagcgtca aaaaccgaaa gggagcataa
     8641 gcgggcgtct tcatccagca gtggactgac aggggggaag cgcagcacac ggcagtttgg
     8701 tgattgttca ctttccacac ccgcaggcag ggcgttatcc agtagtgcca gcagggcttc
     8761 gccattgccg gaaagtgcct gaattgtgac ggtgtcacct aatgctgtaa tgcgcagcgc
     8821 actgtctatc agcagcaggc tttttaaatc atctttgctg tcgatatctg cggattccag
     8881 cagcagcgtt gccggacgat tcccacacaa ctggtgaaaa agcgcagtcg ggttgtcgcg
     8941 ataagcgcct tcgcaggtta gcagttcgag agtcggtttt tgtgtttgca ttgttattct
     9001 ctaattttgt tcaaaaaaaa gcccgctcat taggcgggct gggtatctga ttgctttacg
     9061 catggtgaat acactgcccg tttcaggaag tgcgccacca acctttcagt acgaaaattg
     9121 ctttcattgt cgataccctt tttacgtgaa cttgcgtact agttaactgg ttcgatgatt
     9181 aattgtcaac agctcatttc agaatatttg ccagacccgt tatgatgtcg gcgcaaaaaa
     9241 cattatccag aacgggagtg cgccttgagc gacacgaatt atgcagtgat ttacgacctg
     9301 cacagccata ccacagcttc cgatggctgc ctgacgccag aagcattggt gcaccgtgca
     9361 gtcgagatgc gcgtcggcac cctggcgatc accgaccatg acaccacagc tgcaatcgcg
     9421 ccagcaagag aggaaatttc acgttcggga ctggcgctga atcttattcc cggcgtggaa
     9481 atttccacgg tctgggaaaa tcatgaaatt catattgtcg ggctgaatat tgatattact
     9541 catccgctaa tgtgtgagtt cctcgcgcag cagacagaac ggcgcaatca gcgggcgcag
     9601 caaattgccg aaagacttga aaaagcgcaa atacctgggg cgctggaagg cgcacaacga
     9661 ctggcgcagg gcggcgcagt gacgcgcggt cattttgcgc gtttccttgt ggagtgcggc
     9721 aaagccagtt caatggcaga tgtctttaaa aagtatctgg cgcgcgggaa aaccggatac
     9781 gttccgccac agtggtgtac aatagaacaa gctattgatg tcattcatca ttctggcggt
     9841 aaggcggtgc tagctcatcc tgggcggtac aatcttagtg ctaaatggct gaaaagattg
     9901 gttgcgcatt ttgccgaaca ccacggcgac gcgatggaag tcgcgcagtg ccagcaatcg
     9961 cccaatgaac gcacccagct ggccacactt gcgcgtcagc atcatttatg ggcatcacaa
    10021 ggatctgatt ttcatcagcc atgcccgtgg atcgaactgg gtcgtaaact ctggctgccc
    10081 gcaggcgttg aaggcgtctg gcagctatgg gaacagccgc agaacaccac agagagggaa
    10141 ttatgagcca gtttttttat attcatcctg ataacccgca gcaacgtctg atcaaccagg
    10201 cggtggagat cgtgcgtaaa ggcggggtga ttgtctatcc aactgattcc ggctatgcgc
    10261 tcggctgtaa aattgaagat aaaaacgcca tggagcgtat ttgtcgtatt cgccagctgc
    10321 cggacggtca caactttacc ctgatgtgtc gcgatctttc tgaactctcg acctattcat
    10381 tcgtggataa cgttgcgttt cgtttgatga aaaacaacac gccgggcaac tataccttca
    10441 tcctgaaggg gacgaaggaa gtgccacgcc gtctgttgca ggagaaacgc aaaaccatcg
    10501 gtatgcgtgt gccttctaac ccgattgcgc aggcgttgct tgaagcactg ggcgaaccga
    10561 tgctttccac ttcgctaatg ctgccaggca gcgaatttac cgaatccgat cccgaagaaa
    10621 ttaaagaccg tctggaaaaa caggtagatt tgattattca cggcggctat ctcgggcaga
    10681 aaccaacaac ggttattgat cttaccgatg atacgccggt cgtggtgcgt gaaggcgtag
    10741 gtgatgtgaa gcctttctta taagtgcgta actctgtata ctacgcggcc ttaaaatggc
    10801 gctatacggc gccattgatt ctcctgttcg acgcctgtga aggcgacacc aaaaggaagc
    10861 tctatgagcg aaaaattaca gaaagtgctg gcccgcgctg gccacggctc ccgccgtgaa
    10921 atcgaatcta ttattgaagc cggtcgtgtg agtgttgatg gcaaaattgc taaactcggc
    10981 gatcgcgttg aagttacccc tgggctgaaa attcgtatcg atggtcatct gatttcggta
    11041 cgtgagtccg ctgaacaaat ttgtcgcgtt ctggcctatt acaaaccgga aggtgagttg
    11101 tgtacccgta acgatccgga agggcgtccg acggtgtttg accgtctgcc aaagctgcgt
    11161 ggcgcacgct ggattgccgt aggtcgtctg gacgttaaca cctgtggtct gctgctgttc
    11221 accaccgatg gtgaactggc aaaccgttta atgcatccaa gccgtgaagt tgaacgtgaa
    11281 tacgccgtgc gtgtatttgg tcaggttgac gacgcgaaac tgcgtgattt gagtcgtggc
    11341 gtgcaactgg aagatggtcc ggcagctttt aaaaccatca agttcagcgg cggcgaaggg
    11401 atcaaccagt ggtacaacgt gactctgacc gaaggccgta accgtgaagt tcgtcgtctg
    11461 tgggaagcgg ttggtgtgca ggtgagccgc ctgatccgtg ttcgttacgg tgatatccca
    11521 ctgccaaaag gtctgccacg cggtggctgg acggagctgg acctcgccca gactaactat
    11581 ctgcgcgaac tggtggagct tccgccagaa accagctcta aagtcgctgt agaaaaagac
    11641 cgtcgtcgca tgaaggctaa tcagattcgt cgtgcggtga aacgtcacag tcaggtgagc
    11701 ggcagtcgtc gttctggcgg gcgtaataac ggttaatcaa agtatgccgg acgtcatatc
    11761 cggcattttt acagattaat aatcaatccc tatttgcgcc ttaacaccgg catcaaacgc
    11821 atgtctgatg gggcgcagct cacttaccgt atccgccagt tcaagaatat cccgatgaca
    11881 gcctcgaccc gtgataatca ccgtctgttg atgtggacgt tcatttaacg cctgcaccac
    11941 ttcttccagt ggcaaatagt catacgccac catatacgtc agttcatcaa gcaaaaccat
    12001 atccagtgag gaatcagcaa gcatccgctt tgcatgttgc cagacttcgc ggcaggcggc
    12061 ggtatcagac tcgcggtttt gtgtatccca ggtaaagccc gttgccatca cctgaaactc
    12121 aacgccatgt ggctccagca gattgcgttc gccattaggc caggtgcctt taataaactg
    12181 cacgacgccc actttttttc cgtgaccaac tgcgcgtgtt gccgtaccaa atgccgcggt
    12241 ggttttgcct tttccattgc cggtaaagac gatgataata ccgcgttcat cctgggcctg
    12301 ggccacacga gcatctactt tttctttcac tcgctgctga cgctgttggt agcgttcatc
    12361 actcattggg aaattcctgg tttacggccc ggttgggcgt caaaggtcat gccggtttta
    12421 cggcggctgt catcgcccat cagccagagg tagagcggca tgatatcagc gggtgtttta
    12481 agtttctgtg gatcttcggt cgggaaggcg cttgcgcgca ttgcggtgcg cgtaccgcct
    12541 gggttaatgc agttgacgcg caggcgctgc tgatattcat cggccagtac ctgcatcatc
    12601 ccttcggtgg caaatttcga cgctgcatat gcaccccagt tggctcgtcc ctgacgtcca
    12661 acgcttgatg aagtaaagac cagtgaaccg gcgtccgatt tgagtaataa aggaagcagt
    12721 gcctgggtga gcataaaggt ggcattaacg ttgatctgca tgacgtcctg ccagacctgc
    12781 ggattttgtt cgctcattgg gcaaacatcg ccgagcaatc cggcattatg caaaacacca
    12841 tccagacgcg gataattaac gacaatgcgc tgtgccagtt gttggcaatt ttcggacgtg
    12901 caggtcagca aatcgagaat aaaccactgt ggctgacgcc cagtttcttc gtttatgtgg
    12961 ctggctacct gacgtaattt ttcttcatta cggcccaaca gaatcactgt cgcaccatag
    13021 cgtgcatacg tcatcgcggc ttcacgacca atcccatcgc tggctcccgt caccaggata
    13081 atgcgatcat tgagtaaatc ttgttttggc tggtaatgca tggctactcc tcaacgacgt
    13141 tgtctgtcgc gctgccagtg tactttatga ctttggggct ttatgcctga aacagaaggg
    13201 catttcaatc agcctggggc gataacaccg caaaatggat actttgtcat actttcgctg
    13261 caataacatc tctgcgagac ggcttaacat gcctgttgta aactgtgagc caaagcgttg
    13321 tttaaccaag gtggggactc gtggaattgt tgtctgaata tggtttgttt ttggctaaaa
    13381 tcgttaccgt tgtgctagcg attgcggcga ttgccgccat tattgtcaat gttgctcaac
    13441 gtaataaacg ccagcgtggc gagttacggg tcaacaatct cagcgaacag tataaggaga
    13501 tgaaagaaga actggccgcg gcgctgatgg actcacatca gcaaaaacag tggcacaaag
    13561 cgcagaagaa aaagcacaag caagaagcga aagcagcaaa agcgaaagcc aaactgggcg
    13621 aggtggcaac tgacagtaaa cctcgcgtct gggtgctgga ttttaaaggc agcatggacg
    13681 cccatgaagt gaactcgcta cgtgaagaga taacggcggt actcgcagca ttcaaaccgc
    13741 aggatcaggt tgtgctccgt ctggaaagcc ctggtggcat ggtgcatggt tacgggttgg
    13801 cggcttcgca gctgcagcgt ctgcgtgata aaaacattcc tttaactgtt acggtagaca
    13861 aagtcgctgc cagcggcggt tacatgatgg cctgtgtggc ggacaaaatt gtttccgcac
    13921 cgtttgctat tgtgggttcc attggggtgg tggcgcaaat gcccaacttt aaccgcttcc
    13981 tgaaaagcaa agatattgat atcgaactgc acaccgccgg gcagtataag cgcacgctga
    14041 ccttgctggg tgaaaatacc gaagaagggc gggagaaatt ccgcgaagag ctgaacgaaa
    14101 cgcatcagtt atttaaagat tttgtgaagc gtatgcgtcc gtctctggat attgaacagg
    14161 tggcaacggg tgaacactgg tacggacaac aggcggtaga gaaaggcctg gttgatgaaa
    14221 tcaacaccag tgatgaagtt attcttagcc tgatggaagg ccgtgaagtg gttaatgtac
    14281 gctatatgca gcgtaaacga ctcattgacc gattcaccgg cagcgcggca gagagcgccg
    14341 atcgattgtt gctacgctgg tggcagcggg gtcaaaagcc attgatgtaa aagacaaacg
    14401 cgaggctaag acctcgcgtt ttgctttaat caaccagatg atatttttct gaaagcacat
    14461 gggccaggtg tttgaacata ttaaacaccg cggtgctttt ggctgttggc aatccttgtt
    14521 catctaaaaa gtagtcgccg gtaaatacca gcacgccatt acgctgcgtg acgccggtgg
    14581 cttcaatccc tgcgagcgta tcctcatgct cacgaatgat tttgttggcc tctttcaaca
    14641 gcgtttcgcg gtcgatgggt tgtgtctctt tgttcattat ttactcctta aacaaggaca
    14701 ttagtctacg ccaggcatgg cttgcagaca aatataccac gctggtggca agagcgcctt
    14761 actggcaact ttggattttg catgctaata aagttgcgta tcggatttta tcaggtacag
    14821 tgtgacgctt tcgtcaatct ggcaatagat ttgcttgaca ttcgaccaaa aatccgtcgt
    14881 gctatagcgc ctgtaggcca agacctgtta actcagtcac ctgaattttc gtgaacagag
    14941 tcacgacaag gggttgatat ccgcagagag cgagtccata tcggtaactc gttgccagtg
    15001 gaaggtttat caacgtgcga cgcattcctg gaagaatcaa attaggtaag gtgaatatgg
    15061 gtaaagctct tgtcatcgtt gagtccccgg caaaagccaa aacgatcaac aagtatctgg
    15121 gttgtgacta cgtggtgaaa tccagcgtcg gtcacatccg cgatttgccg accagtggct
    15181 cagctgccaa aaagagtgcc gactctacct ccaccaagac ggctaaaaag cctaaaaagg
    15241 atgaacgtgg cgctctcgtc aaccgtatgg gggttgaccc gtggcacaat tgggaggcgc
    15301 actatgaagt gttgcctggt aaagagaagg tcgtctctga actgaaacaa ctggctgaaa
    15361 aagccgacca catctatctc gcaaccgacc ttgaccgcga aggggaagcc attgcatggc
    15421 acctgcggga agtgattggt ggtgatgatg cgcgctatag ccgagtggtg tttaacgaaa
    15481 ttactaaaaa cgcgatccgc caggcattta acaaaccggg tgagctgaat attgatcgtg
    15541 ttaatgccca gcaggcgcgt cgctttatgg accgcgtggt ggggtatatg gtttcgccgc
    15601 tgctgtggaa aaagatcgct cgtggtctgt ctgccggtcg tgtgcagtcg gtggcggtcc
    15661 gcctggtggt agagcgtgag cgtgaaatta aagcgttcgt accggaagag ttctgggaag
    15721 tcgatgccag cacgactacg ccatctggtg aagcgttgga gttgcaggtg actcatcaga
    15781 atgacaaacc gttccgtccg gtcaacaaag aacaaactca ggctgcggta agtctgctgg
    15841 aaaaagcgcg ctacagcgtg ctggaacgtg aagacaaacc gacaaccagt aaacctggcg
    15901 ctccttttat tacctctacg ctgcaacaag ctgccagcac ccgtcttggt tttggcgtga
    15961 aaaaaaccat gatgatggcg cagcgtttgt atgaagcagg ctatatcact tacatgcgta
    16021 ccgactccac taacctgagt caggacgcgg taaatatggt tcgcggttat atcagcgata
    16081 attttggtaa gaaatatctg ccggaaagtc cgaatcagta cgccagcaaa gaaaactcac
    16141 aggaagcgca cgaagcgatt cgtccttctg acgtcaatgt gatggcggaa tcgctgaagg
    16201 atatggaagc agatgcgcag aaactgtacc agttaatctg gcgtcagttc gttgcctgcc
    16261 agatgacccc agcgaaatat gactccacga cgctgaccgt tggtgcgggc gatttccgcc
    16321 tgaaagcacg cggtcgtatt ttgcgctttg atggctggac aaaagtgatg cctgcactgc
    16381 gtaaaggcga tgaagatcgt atcttaccgg cagtcgataa aggcgatgct ctgacgctcg
    16441 ttgaactaac accagcccag cactttacca agccgccagc ccgtttcagt gaagcatcgc
    16501 tggttaaaga actggaaaaa cgtggtatcg gtcgtccgtc tacctatgcg tcgatcattt
    16561 cgaccattca ggatcgtggc tatgtgcgag tagaaaatcg tcgtttctat gcggaaaaaa
    16621 tgggcgaaat cgtcaccgat cgccttgaag aaaatttccg cgagttaatg aactacgact
    16681 ttaccgcgca gatggaaaac agcctcgacc aggtggcaaa tcacgaagca gagtggaaag
    16741 ctgtactgga tcacttcttc tcggatttca cccagcagtt agataaagct gaaaaagatc
    16801 cggaagaggg tggtatgcgc ccgaaccaga tggttctgac cagcattgac tgcccgactt
    16861 gtggtcgcaa aatggggatt cgcacagcga gtaccggggt attccttggc tgttctggct
    16921 atgcgctgcc gccgaaagag cgttgcaaaa ccaccattaa cctggtgccg gaaaacgaag
    16981 tgctgaacgt gctggaaggc gaagatgctg aaaccaacgc gctgcgcgca aaacgtcgtt
    17041 gcccgaaatg cggcacggcg atggacagct atctcatcga tccgaaacgt aagttgcatg
    17101 tctgtggtaa taacccaacc tgcgacggtt acgagatcga agagggcgaa ttccgcatta
    17161 aaggttatga cggcccgatc gttgagtgtg aaaaatgtgg ctctgaaatg cacctgaaaa
    17221 tggggcgttt cggtaaatac atggcctgca ccaacgaaga gtgtaaaaac acacgtaaga
    17281 ttttacgtaa cggtgaagtg gcaccaccga aagaagatcc ggtgccatta cctgagctgc
    17341 cgtgcgaaaa atcagatgct tatttcgtgc tgcgtgacgg tgctgccggt gtgttcctgg
    17401 ctgccaacac tttcccgaaa tcgcgtgaaa cgcgtgcgcc actggtggaa gagctttatc
    17461 gcttccgcga ccgtctgccg gaaaaactgc gttatctggc cgatgcgcca cagcaggatc
    17521 cggaaggtaa taagaccatg gttcgcttta gccgtaaaac caaacagcaa tatgtctctt
    17581 cggaaaaaga cggaaaggcg actggctggt cagcatttta tgttgatggc aaatgggttg
    17641 aaggaaaaaa ataaccttta attctgtcag gtttttataa acaaagggtc gcgaaagcgg
    17701 ccctttttta t
//
LOCUS       DAZOZX010000081        17386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZOZX010000081 DAZOZX010000000
VERSION     DAZOZX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.081"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="ACHUPT_002639"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="ACHUPT_002639"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0820167.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="ACHUPT_002640"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="ACHUPT_002640"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0820168.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="ACHUPT_002641"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="ACHUPT_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0820169.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="ACHUPT_002642"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="ACHUPT_002642"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0820170.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="ACHUPT_002643"
     CDS             complement(5465..5662)
                     /locus_tag="ACHUPT_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0820171.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="ACHUPT_002644"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="ACHUPT_002644"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0820172.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="ACHUPT_002645"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="ACHUPT_002645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0820173.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="ACHUPT_002646"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="ACHUPT_002646"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0820174.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="ACHUPT_002647"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="ACHUPT_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0820175.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="ACHUPT_002648"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="ACHUPT_002648"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0820176.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="ACHUPT_002649"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="ACHUPT_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0820177.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="ACHUPT_002650"
     CDS             complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="ACHUPT_002650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0820178.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="ACHUPT_002651"
     CDS             complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="ACHUPT_002651"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0820179.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="ACHUPT_002652"
     CDS             complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="ACHUPT_002652"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0820180.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15981..17120
                     /gene="queG"
                     /locus_tag="ACHUPT_002653"
     CDS             15981..17120
                     /gene="queG"
                     /locus_tag="ACHUPT_002653"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0820181.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17103..17156)
                     /locus_tag="ACHUPT_002654"
     CDS             complement(17103..17156)
                     /locus_tag="ACHUPT_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820182.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3943 a         4959 c         4367 g         4117 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga caacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggaata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taaggacaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgtg
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13621 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13681 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13741 cagattattg ccgctgaaca acaacggcag tccctgaatc atgcccgttt gtttgatatc
    13801 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13861 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13921 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13981 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14041 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14101 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14161 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14221 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    14281 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    14341 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14401 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14461 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14521 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14581 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14641 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14701 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14761 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14821 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt
    14881 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14941 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    15001 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    15061 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    15121 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15181 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    15241 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15301 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    15361 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    15421 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15481 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15541 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15601 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15661 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15721 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15781 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15841 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15901 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15961 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16021 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16081 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16141 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16201 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16261 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16321 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16381 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    16441 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    16501 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16561 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16621 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16681 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16741 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16801 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16861 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16921 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16981 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    17041 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17101 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17161 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct tgatttttaa
    17221 cttatttatc aataggttaa aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag
    17281 attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    17341 cagcaataac gcatcgcgtc gctgtagata ttttattgag agaaga
//
LOCUS       DAZOZX010000082        17373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZOZX010000082 DAZOZX010000000
VERSION     DAZOZX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.082"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHUPT_002655"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHUPT_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0820183.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHUPT_002656"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHUPT_002656"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0820184.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHUPT_002657"
     CDS             2826..3980
                     /locus_tag="ACHUPT_002657"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0820185.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHUPT_002658"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHUPT_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0820186.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHUPT_002659"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHUPT_002659"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0820187.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHUPT_002660"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHUPT_002660"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0820188.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHUPT_002661"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHUPT_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0820189.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHUPT_002662"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHUPT_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0820190.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHUPT_002663"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHUPT_002663"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0820191.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHUPT_002664"
     CDS             11296..11511
                     /locus_tag="ACHUPT_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0820192.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHUPT_002665"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHUPT_002665"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0820193.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHUPT_002666"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHUPT_002666"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0820194.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHUPT_002667"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHUPT_002667"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0820195.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHUPT_002668"
     CDS             complement(16247..16786)
                     /locus_tag="ACHUPT_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0820196.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHUPT_002669"
     CDS             complement(16802..17320)
                     /locus_tag="ACHUPT_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0820197.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4028 a         4320 c         4824 g         4201 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa aga
//
LOCUS       DAZOZX010000083        17144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZOZX010000083 DAZOZX010000000
VERSION     DAZOZX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.083"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHUPT_002670"
     CDS             <1..510
                     /locus_tag="ACHUPT_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0820198.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUPT_002671"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUPT_002671"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0820199.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUPT_002672"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUPT_002672"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0820200.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHUPT_002673"
     CDS             complement(2878..3075)
                     /locus_tag="ACHUPT_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0820201.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUPT_002674"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUPT_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0820202.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUPT_002675"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUPT_002675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0820203.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUPT_002676"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUPT_002676"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0820204.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUPT_002677"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUPT_002677"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0820205.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUPT_002678"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUPT_002678"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0820206.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUPT_002679"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUPT_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUPT_002680"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUPT_002680"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0820207.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUPT_002681"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUPT_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0820208.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUPT_002682"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUPT_002682"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0820209.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUPT_002683"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUPT_002683"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0820210.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUPT_002684"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUPT_002684"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0820211.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPT_002685"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPT_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         4007 a         4874 c         4453 g         3810 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta attatgggta
    17101 atgactccaa cttactgata gtgttttatg ttcagataat gccc
//
LOCUS       DAZOZX010000084        16420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZOZX010000084 DAZOZX010000000
VERSION     DAZOZX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.084"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /locus_tag="ACHUPT_002686"
     CDS             complement(<1..1025)
                     /locus_tag="ACHUPT_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0820212.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHUPT_002687"
     CDS             complement(1127..2107)
                     /locus_tag="ACHUPT_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR0820213.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHUPT_002688"
     CDS             complement(2115..2825)
                     /locus_tag="ACHUPT_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0820214.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUPT_002689"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUPT_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0820215.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUPT_002690"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUPT_002690"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0820216.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUPT_002691"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUPT_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0820217.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUPT_002692"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUPT_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0820218.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHUPT_002693"
     CDS             9402..9512
                     /locus_tag="ACHUPT_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0820219.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUPT_002694"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUPT_002694"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0820220.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUPT_002695"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUPT_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0820221.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHUPT_002696"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHUPT_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUPT_002697"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUPT_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0820222.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUPT_002698"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUPT_002698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0820223.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         3974 a         4230 c         4022 g         4194 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa
//
LOCUS       DAZOZX010000085        16227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZOZX010000085 DAZOZX010000000
VERSION     DAZOZX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.085"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUPT_002699"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUPT_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUPT_002700"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUPT_002700"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0820224.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUPT_002701"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUPT_002701"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0820225.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUPT_002702"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUPT_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUPT_002703"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUPT_002703"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0820226.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHUPT_002704"
     CDS             complement(5877..6218)
                     /locus_tag="ACHUPT_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0820227.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUPT_002705"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUPT_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0820228.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUPT_002706"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUPT_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0820229.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUPT_002707"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUPT_002707"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0820230.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHUPT_002708"
     CDS             12897..14240
                     /locus_tag="ACHUPT_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0820231.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHUPT_002709"
     CDS             complement(14302..14508)
                     /locus_tag="ACHUPT_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0820232.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHUPT_002710"
     CDS             14833..15387
                     /locus_tag="ACHUPT_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0820233.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>16227)
                     /locus_tag="ACHUPT_002711"
     CDS             complement(15450..>16227)
                     /locus_tag="ACHUPT_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820234.1"
                     /translation="ESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYT
                     ITLRGSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKG
                     YIRQAFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVI
                     ESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANL
                     AADSNITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3869 a         4427 c         3969 g         3962 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcc
//
LOCUS       DAZOZX010000086        15886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZOZX010000086 DAZOZX010000000
VERSION     DAZOZX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.086"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="ACHUPT_002712"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="ACHUPT_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0820235.1"
                     /translation="RAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLG"
     gene            561..755
                     /gene="bfd"
                     /locus_tag="ACHUPT_002713"
     CDS             561..755
                     /gene="bfd"
                     /locus_tag="ACHUPT_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR0820236.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            828..1304
                     /gene="bfr"
                     /locus_tag="ACHUPT_002714"
     CDS             828..1304
                     /gene="bfr"
                     /locus_tag="ACHUPT_002714"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR0820237.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1301..1768)
                     /locus_tag="ACHUPT_002715"
                     /pseudo
     CDS             complement(1301..1768)
                     /locus_tag="ACHUPT_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2147..2458
                     /gene="rpsJ"
                     /locus_tag="ACHUPT_002716"
     CDS             2147..2458
                     /gene="rpsJ"
                     /locus_tag="ACHUPT_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0820238.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2491..3120
                     /gene="rplC"
                     /locus_tag="ACHUPT_002717"
     CDS             2491..3120
                     /gene="rplC"
                     /locus_tag="ACHUPT_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0820239.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3131..3736
                     /gene="rplD"
                     /locus_tag="ACHUPT_002718"
     CDS             3131..3736
                     /gene="rplD"
                     /locus_tag="ACHUPT_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0820240.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3733..4035
                     /gene="rplW"
                     /locus_tag="ACHUPT_002719"
     CDS             3733..4035
                     /gene="rplW"
                     /locus_tag="ACHUPT_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0820241.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4053..4874
                     /gene="rplB"
                     /locus_tag="ACHUPT_002720"
     CDS             4053..4874
                     /gene="rplB"
                     /locus_tag="ACHUPT_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0820242.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4891..5169
                     /gene="rpsS"
                     /locus_tag="ACHUPT_002721"
     CDS             4891..5169
                     /gene="rpsS"
                     /locus_tag="ACHUPT_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0820243.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5184..5516
                     /gene="rplV"
                     /locus_tag="ACHUPT_002722"
     CDS             5184..5516
                     /gene="rplV"
                     /locus_tag="ACHUPT_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0820244.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5534..6235
                     /gene="rpsC"
                     /locus_tag="ACHUPT_002723"
     CDS             5534..6235
                     /gene="rpsC"
                     /locus_tag="ACHUPT_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0820245.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6248..6658
                     /gene="rplP"
                     /locus_tag="ACHUPT_002724"
     CDS             6248..6658
                     /gene="rplP"
                     /locus_tag="ACHUPT_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0820246.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6658..6849
                     /gene="rpmC"
                     /locus_tag="ACHUPT_002725"
     CDS             6658..6849
                     /gene="rpmC"
                     /locus_tag="ACHUPT_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0820247.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6849..7103
                     /gene="rpsQ"
                     /locus_tag="ACHUPT_002726"
     CDS             6849..7103
                     /gene="rpsQ"
                     /locus_tag="ACHUPT_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0820248.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7268..7639
                     /gene="rplN"
                     /locus_tag="ACHUPT_002727"
     CDS             7268..7639
                     /gene="rplN"
                     /locus_tag="ACHUPT_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0820249.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7650..7964
                     /gene="rplX"
                     /locus_tag="ACHUPT_002728"
     CDS             7650..7964
                     /gene="rplX"
                     /locus_tag="ACHUPT_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0820250.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7979..8518
                     /gene="rplE"
                     /locus_tag="ACHUPT_002729"
     CDS             7979..8518
                     /gene="rplE"
                     /locus_tag="ACHUPT_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0820251.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8533..8838
                     /gene="rpsN"
                     /locus_tag="ACHUPT_002730"
     CDS             8533..8838
                     /gene="rpsN"
                     /locus_tag="ACHUPT_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0820252.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8872..9264
                     /gene="rpsH"
                     /locus_tag="ACHUPT_002731"
     CDS             8872..9264
                     /gene="rpsH"
                     /locus_tag="ACHUPT_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0820253.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9277..9810
                     /gene="rplF"
                     /locus_tag="ACHUPT_002732"
     CDS             9277..9810
                     /gene="rplF"
                     /locus_tag="ACHUPT_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0820254.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9820..10173
                     /gene="rplR"
                     /locus_tag="ACHUPT_002733"
     CDS             9820..10173
                     /gene="rplR"
                     /locus_tag="ACHUPT_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0820255.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10188..10691
                     /gene="rpsE"
                     /locus_tag="ACHUPT_002734"
     CDS             10188..10691
                     /gene="rpsE"
                     /locus_tag="ACHUPT_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0820256.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10695..10874
                     /gene="rpmD"
                     /locus_tag="ACHUPT_002735"
     CDS             10695..10874
                     /gene="rpmD"
                     /locus_tag="ACHUPT_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0820257.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10878..11312
                     /gene="rplO"
                     /locus_tag="ACHUPT_002736"
     CDS             10878..11312
                     /gene="rplO"
                     /locus_tag="ACHUPT_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0820258.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11320..12651
                     /gene="secY"
                     /locus_tag="ACHUPT_002737"
     CDS             11320..12651
                     /gene="secY"
                     /locus_tag="ACHUPT_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0820259.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12683..12799
                     /gene="rpmJ"
                     /locus_tag="ACHUPT_002738"
     CDS             12683..12799
                     /gene="rpmJ"
                     /locus_tag="ACHUPT_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0820260.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12946..13302
                     /gene="rpsM"
                     /locus_tag="ACHUPT_002739"
     CDS             12946..13302
                     /gene="rpsM"
                     /locus_tag="ACHUPT_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0820261.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13319..13708
                     /gene="rpsK"
                     /locus_tag="ACHUPT_002740"
     CDS             13319..13708
                     /gene="rpsK"
                     /locus_tag="ACHUPT_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0820262.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13742..14362
                     /gene="rpsD"
                     /locus_tag="ACHUPT_002741"
     CDS             13742..14362
                     /gene="rpsD"
                     /locus_tag="ACHUPT_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0820263.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14388..15377
                     /gene="rpoA"
                     /locus_tag="ACHUPT_002742"
     CDS             14388..15377
                     /gene="rpoA"
                     /locus_tag="ACHUPT_002742"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0820264.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15418..15801
                     /gene="rplQ"
                     /locus_tag="ACHUPT_002743"
     CDS             15418..15801
                     /gene="rplQ"
                     /locus_tag="ACHUPT_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0820265.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4089 a         3734 c         4234 g         3829 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggccgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt
      361 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
      421 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      481 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      541 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      601 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      661 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      721 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      781 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      841 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      901 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      961 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
     1021 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
     1081 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
     1141 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
     1201 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
     1261 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
     1321 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1381 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1441 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1501 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1561 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1621 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1681 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1741 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1801 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1861 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1921 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1981 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     2041 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     2101 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     2161 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     2221 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     2281 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     2341 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2401 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2461 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2521 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2581 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2641 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2701 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2761 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2821 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2881 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2941 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     3001 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     3061 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     3121 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     3181 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     3241 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     3301 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3361 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3421 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3481 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3541 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3601 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3661 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3721 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3781 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3841 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3901 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3961 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     4021 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     4081 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     4141 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     4201 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     4261 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     4321 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4381 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4441 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4501 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4561 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4621 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4681 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4741 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4801 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4861 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4921 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4981 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     5041 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     5101 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     5161 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     5221 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     5281 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     5341 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5401 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5461 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5521 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5581 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5641 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5701 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5761 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5821 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5881 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5941 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     6001 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     6061 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     6121 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     6181 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     6241 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     6301 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6361 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6421 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6481 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6541 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6601 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6661 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6721 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6781 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6841 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6901 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6961 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     7021 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     7081 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     7141 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     7201 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     7261 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     7321 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7381 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7441 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7501 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7561 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7621 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7681 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7741 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7801 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7861 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7921 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7981 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     8041 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     8101 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     8161 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     8221 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     8281 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     8341 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8401 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8461 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8521 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8581 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8641 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8701 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8761 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8821 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8881 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8941 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     9001 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     9061 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     9121 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     9181 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     9241 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     9301 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9361 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9421 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9481 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9541 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9601 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9661 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9721 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9781 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9841 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9901 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9961 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
    10021 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
    10081 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
    10141 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
    10201 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
    10261 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
    10321 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10381 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10441 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10501 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10561 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10621 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10681 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10741 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10801 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10861 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10921 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10981 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    11041 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    11101 ccgaagttcg tctatctgac ttggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    11161 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    11221 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    11281 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    11341 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11401 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11461 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11521 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11581 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11641 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11701 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11761 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11821 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11881 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11941 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    12001 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    12061 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    12121 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    12181 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    12241 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    12301 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12361 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12421 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12481 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12541 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12601 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12661 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12721 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12781 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12841 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12901 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12961 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    13021 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    13081 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    13141 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    13201 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    13261 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    13321 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13381 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13441 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13501 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13561 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13621 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13681 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13741 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13801 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13861 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13921 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13981 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    14041 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    14101 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    14161 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    14221 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    14281 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    14341 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14401 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14461 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14521 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14581 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14641 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14701 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14761 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14821 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14881 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14941 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    15001 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    15061 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    15121 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    15181 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    15241 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    15301 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15361 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15421 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15481 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15541 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15601 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15661 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15721 ttccgtgcag gcgacaacgc gccgatggct tatatcgagc tggttgatcg ttcagagaaa
    15781 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15841 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAZOZX010000087        15864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZOZX010000087 DAZOZX010000000
VERSION     DAZOZX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.087"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..756
                     /locus_tag="ACHUPT_002744"
     CDS             <1..756
                     /locus_tag="ACHUPT_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR0820266.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHUPT_002745"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHUPT_002745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0820267.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="ACHUPT_002746"
     CDS             complement(1792..1995)
                     /locus_tag="ACHUPT_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0820268.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHUPT_002747"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHUPT_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0820269.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="ACHUPT_002748"
     CDS             complement(4588..5100)
                     /locus_tag="ACHUPT_002748"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0820270.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHUPT_002749"
                     /pseudo
     CDS             complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHUPT_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6903..7840)
                     /locus_tag="ACHUPT_002750"
                     /pseudo
     CDS             complement(6903..7840)
                     /locus_tag="ACHUPT_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHUPT_002751"
     CDS             complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHUPT_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0820271.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHUPT_002752"
     CDS             complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHUPT_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0820272.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHUPT_002753"
     CDS             complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHUPT_002753"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0820273.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHUPT_002754"
     CDS             complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHUPT_002754"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0820274.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11829..12209)
                     /locus_tag="ACHUPT_002755"
     CDS             complement(11829..12209)
                     /locus_tag="ACHUPT_002755"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0820275.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHUPT_002756"
     CDS             complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHUPT_002756"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0820276.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHUPT_002757"
     CDS             complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHUPT_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0820277.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHUPT_002758"
     CDS             complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHUPT_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0820278.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
BASE COUNT         3734 a         4143 c         4156 g         3831 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcggtggg ttaacaatcg cgtggttaaa gtagaggcca gtttcctgaa
     6241 tacgggtata ccagttacgg gcgttctgct cgaactgacc gtaaaagccc ggattcgcgc
     6301 ccagtgcgca accgaaagcg gctttgtagt ctggggtacg gcccatccag ccatagctca
     6361 ggcgtgacca ttcagcgatg gcgtcgcgtt gctggcgcag gtcgtcggca cttttcgcca
     6421 cgcggaagaa tttatgggta tagccgccgc tgccggtgtc ggtgttccaa cacagagagt
     6481 cctgcatctc cggtttgtgc agcgcgtcgt acagttgggc aacagacgct gccgcattac
     6541 gaaatgccgg atgagtggtg acgtctttca ctcgctcgcc atagatatag atctcgcgac
     6601 catcctgcag gcttttcaga tactcttccc cggtgaaagg acgttgggta ctggcgcgga
     6661 aatcttctgg tttcatgtcg acctctacag tattaatcgg aatacgattg ttaattttgt
     6721 gtgttgtttt tgttatttaa ttgaagcgtt gaacgcggtt gtcgggaaga gacgtttcag
     6781 gcgaacgttg gtacttttcg acagcaaggt ggagttaaat agtaaactgc gatcgtgatc
     6841 aacttacagt aaggcaaatc ataatattcc ccgctaacaa gggctaacgg ggacggaatt
     6901 ttttatgggg cgaatggaat ggtaatcgtc atattttcat gaccaacgac gataggacct
     6961 gaatagtttt ctgtgcttct tgcttccatg tttcatccgg aacggtgatt ggtaccaggt
     7021 ggctaaggat gagttttttc acttttgctt cattggcaat tttgcctaca tcctcaatag
     7081 tcgtatggtg agaagcaatg gcttttgcca gtgttttacc attgccaata ctgtctgcca
     7141 gcttagcgac ggagggctca tgtacggcct catgcacaag tatatcagcg ccttctgaga
     7201 aagttgccag tgggggaaag tacgaggtat ctcctgagaa gacaattcgc ttgccttgtg
     7261 tgtcgaagcg ataagcaaaa gcctcaccgt cagggaaagg agaatgaggg acctttaatg
     7321 cagtcacggt cacggtatca tctttataaa ctacgccttc accaacatct tgagctgtaa
     7381 cgagtttgcg aggatcgggt tttccttctt cttccatacg cagtgtgatg tcccgatcga
     7441 agacctgcca actggcagcg gtcatggctc gggtgcctgg cggaccgaat acatgaatag
     7501 ttttgtcctg cagaccgcta gcccatgcat tcattaagac tgatggatag tccagaatat
     7561 gatcactgtg taaatgggtg agtaaaatat tatcaatctg gcgcagtgga atattatttt
     7621 ttaccagttg taagctggcc ccgtaaccag catcaattaa ccaggtttta ttgccaataa
     7681 taagtgccgt tgccatcggt aaacggtcag cttttaataa agaagggcca cctttcgttc
     7741 caagtagtac aattttcatt tattgcggtt cggaggcgta ggtgaatgcc actggcgcaa
     7801 tcaggcacgc cacaaatagt gagcgtaaag atattttcat gtcgagtctc tcaggttgat
     7861 aaatggtcag cgaccacgga attgtgttgg tgagcaaccc atcattcggt tgaaaaaacg
     7921 ggcgaaatag gcgggatctt tgtagccaag atcaagggct atctggttga tgctttgctc
     7981 gctatagagc agaagccgtc tggcttcccg gctcactcgt tcgaaaatca atctcttcgg
     8041 cgattgattg gcaaaacgtc ggcacagctc ggtaagccgt gattcactca taccgagcat
     8101 tgttgcatat tcagggacca ttaaatgttg gcgataattt tcgtcaatca gccgattaaa
     8161 cttctggaat aaacacatct cgccgcgcac gctgcatgtt gaaatatcat ttggtggtgc
     8221 ttcacgcagt aggattgtaa aaatactttg cgccagtgat tgcaatagaa tttcccgtcc
     8281 ttgcttattt tgttgaaact ccagggatat tatcggccaa taatgattta atgccgccag
     8341 tgtatgttcc attccctcca gtgacagaca gataccttgt agcattgcgg catctccact
     8401 accaggccag agcttttcta ccaggggcca aattaactcc tgacgtacgg tcagaacatg
     8461 cccatcgcta tcaggatcag taaagaaagt atgtggcacc gatggtggtg ttagtacaaa
     8521 aagcggtgcg cgcacagaat agaagtgctc atccagttgt aaagtaatgc gtccagtgac
     8581 cagataatgg agttgaaaat atctatcgtg ccagtgaggg cgcatatcgc gtccaaagaa
     8641 attcgctaac cgggcaaatg tttcataatg cacatcttca ccagcgtagc gggcatcata
     8701 ttcccggacc aaatcaatat tttcataacc atgttcaaag atagcttcac tggacatcat
     8761 gactccttat tgcggttttg gtgcgataag ttcagatgaa agagagtgtt cccgactccc
     8821 catagggatt cgggtcatga ctaacgcgcc cagtaccagc aatcctgcta caaaccacaa
     8881 acctgagcta aaactgccgg ttgcatcttt caaaataccg ataagtaatg gactgactgc
     8941 tgaacctaca ttgccgacag cattgatgac agccatcgca acggcacgcg attgcaagct
     9001 tataacttga tccggtgttg tccaaaagat agtcatcgcg gtaaatgatc ccattgaggc
     9061 cataatgatg ctgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     9121 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     9181 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     9241 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     9301 attaatcgca ctcagagtat tagtcgggca aaaataagca agtgtatata gcatgatggc
     9361 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     9421 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     9481 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     9541 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     9601 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     9661 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     9721 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     9781 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     9841 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     9901 cgtgacataa aagagtgttg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     9961 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
    10021 aataaaaaga aaaatgatta ggcgacgaaa taatttatta atgacacgat gttcaatttc
    10081 aacagtttta ttatctttat attgcagggt agaggtcgtc atgatgcgct ccacatcgta
    10141 cgtttagtag acagagttgt tgttttgctt cgccatcgaa atagaagaac tattaccaaa
    10201 ccgtgctgcc agcgcttcgg cggcgcgggc gagcagggtg gtgtcaacgc cgacggcgac
    10261 aaacagcgcg cccagttcca gatagcgttt tgccagttgc tcattggcga tcaggatccc
    10321 cggcgctttg cccgcttcgc ggatctgcac gatcgcctgc tcaatggcgg cctgtacttc
    10381 cgggtgctgc ggattaccgg cataacccat atcggcgctc agatccgccg ggccgataaa
    10441 cacgccgtcg accccttcca cgtccagaat ctgcggtaag ttcttcattg cctcacgcgt
    10501 ttcgatctgc accagcacgc acatttgatc gttggctttt tgcaggtaat caggaatgcg
    10561 attccagcgc gaggcgcgag ccagcgcact gcccacaccg cgaataccgg cggggggata
    10621 acgggtggcg cgtaccgctt tacgggcttc gtcggcgttt tgtaccatcg gcaccagtaa
    10681 ggtttgtgtg cctacgtcca gcagttgttt gatttgcacc ggatcgttcc acgatgggcg
    10741 taccactggc tggctggggt agggcgcaat cgcctgtagt tgggtgagca cggtttgcac
    10801 gttattcggc gcgtgctcac cgtcgatcaa taaccagtcg aatcctggtc cggccagtaa
    10861 ctccgcgctg tagctgctgc tcagccccag ccataatcca atctgcggac ggcctgcttt
    10921 cagcgccgct ttaaaactgt tttccatcgt tctctcctta aacaaagcgg cagctaatgg
    10981 agcccatgtt gccgtaatcg acgtggaagg tgtcgccctt acgcgccgga accgggaggg
    11041 tgaacgaacc accgagaatg atttgcccgg tttccagttg tacgtcatac ggtgcgagtt
    11101 tgttcgccag ccaggccacg ccgtttgccg gatgattcag cacaccagcg gcgacgccgg
    11161 tttcttcaat cacgccattg cgatacatca gggcggagat ccaacgcaga tccagttcgt
    11221 ctggtttgat cggacgacct ccgaggatca ctccggcgtt ggcggcgtta tcggaaatgg
    11281 tgtcgaatac tttgcgcggg cgctgggttt ccggatcgat gttgtggcag cgagcgtcga
    11341 tcagctccag cgccgggatc acatagtccg tggcgttgta aacgtcgaac agcgtgcagt
    11401 ttggcccacg cagcggtttt gccagcacaa aagccagctc cacttcaatg cgcggcacga
    11461 taaagcgatc ggtcgggata tcgctgccat cgtggaagaa catgtcgtcc agcagtgcac
    11521 cgtaatccgg ttcgctgatc tgcgagcttg cctgcatcgc tttcgaggtc agaccgattt
    11581 tgtggccttt cagcgtgcga ctttcggcga ttttcaggcg aacccattcg cgctgcacgg
    11641 cgtaggcgtc ttcgatggtg atctccgggt aatccagcga gatcgcgcgg atctgttcgc
    11701 gctgtttttc tgcctgatcc agacgctggg cgatcagggt gtgggtgtgt ttatcgaaca
    11761 tagcgatatc ctgtggcatt gccggatgcg gcagcgcctt atcatccggc ctggaagctg
    11821 gtgcaaattt atttaaacaa cgcgtgtacg ttgttctgtt tgaaattcaa cgtcgggtgc
    11881 agctctgcaa tctcaaatga aagcgctagg taacggctct ccatcagtga ggcgaagtgc
    11941 gttttaatca gatcaaacag catttcgccc acttcctgac ggctttccag actgcgtccg
    12001 ctgccgattt ttagcgtcat atgcacaaag gcgtaatcgt gcttgccatc agccatttgc
    12061 caggtatcga tccagtgcgc ccggctacgt attcccccaa gcggaaaaat ccccgttgcc
    12121 gccagtgcag tgttaacact ggcaaacagc tcaggtaagc gggcttcttc acgaatattt
    12181 tcagtacatt ccacgataaa gtgcggcata aaacgtcctt acgcgggcag cgggaaaaca
    12241 gcgttaacct gaccggtgcc agagcttggg aacagctcgg taataaactc caccttgccg
    12301 tcgtatttat cccagccgag catccccagc agcatcaccg tgtcgtgcat attgccttcg
    12361 ccgtagcagt agtcggcgta ctccggcagc atattgcaaa actctttgaa ctggccttcg
    12421 cgccacagct tcaccacacg ctcgtccatc tggcggtcga actcgcgggt gtagctgttc
    12481 atcccttctt ccgcacgctg atcgtcaata aagcggtgcg ataacgaacc gctggcaagg
    12541 accgccacgg tgccgtcgta ctgttcgatc gctttcagaa tcgcttcgcc cagcttgcgg
    12601 ctgtcggcaa aatcgtgaac cgtgcagaaa gccgaaatgg agaccacttt gaagtgcttg
    12661 tcttcattca tgtagcgcat cggtaccagc gtgccgtatt ccagtttcag gctgggaata
    12721 ttgtgcgctt ttgcccgcac gccgagcttt aaggcttcct cggcaattag ctgccccaac
    12781 tccgggttgc cctcgtagtt gtaggtcatg tcgcgaataa aatgcggcag ctcgttgctg
    12841 gtgtagacgc cttcaaaatg gtctgcacag ttgatgtgat aagcactgtt gaccagccag
    12901 tgagtatcga aaacgataat ggtatcgacg cccatttccc ggcaacgctt gctgatctct
    12961 ttatgcccgt cgatcgcacc ctggcgacaa ccgtggtttt ttcctggtag ttcagagaga
    13021 tacatcgacg gtacgtgcgt gatcttggcg gctaacgcta acttacccat atcagactcc
    13081 ccatttcgga attggatggt cgcccatgga aatgcagacg ttcttcattt ccgcgaacac
    13141 ttcgaagctg tactcaccgc cttcacgccc ggtgccggag gcttttacgc cgccaaatgg
    13201 ctggcgcagg tcgcgcacgt tctgggtgtt gacgaacacc atgcctgctt caatgccacg
    13261 cgccaggcgt aacactttgc tgacatcctg tgtccagatg tacgacgcga ggccgtactc
    13321 cacgtcgttt gccaggcgta agccttcggc ttcgtcttta aacggcagca ggcaggcgac
    13381 cggcccgaaa atctcttcct gggcaacgcg catacggtta tctacgtccg ccagcaccgt
    13441 tgggcgcagg aagttgccgc ctttcaggtg cgcaggcagg tcggacggtt tatccgggcc
    13501 gcccgccaac agggttgcgc cttcttcaat gccgagacgg atatagccgg agactttttc
    13561 ccagtgttgc tgactgataa gcgccctaac ctgggtattc ggatcgttcg gatcgcccac
    13621 gcgcagacgg ttggcgcgtt cggcaaagcg tttaacgaat tctggataga tgctctgctg
    13681 aataaagatg cgcgaaccgg cggtgcagcg ttcgccgttg attgagaaga tggtgaacag
    13741 ggcggcgtcc agcgcacgct caatatcggc atcttcaaaa atcagcaccg gtgatttacc
    13801 gcccagctcc atggagtatt ttttcagccc ggcgtttttc atgatgttgc gcccggtggc
    13861 ggtaccgccg gtgaacgaca cggcacgcac gtcatgatga cggaccagcg catcgcctgc
    13921 ggttgcgccg tagccctgta ccacgtttag cacgcccgcc ggaataccag cttccagcgc
    13981 cagctcaccc aggcggtcgg cggtcagcgg ggagagttcc gacatcttca gcaccgcggt
    14041 attgcccagc gccagacacg gcgcgacctt ccaggtggcg gtcataaacg gcacgttcca
    14101 cggcgacgcc agcgcacaaa cgcctaccgg ttgcaccagc gtgtagttga gcatcttgtc
    14161 gtcaaccgga taggtcttgc cgttcatctg ctggcagact tccgcgaaaa attcaaagtt
    14221 gtgagaagcg cgtgggatca acacattttt ggtctgatgg atcggcaggc ccgtgtccgc
    14281 ggtttccatc gcggcgatct caggcacgtt ctggtcgatc agatcgccca gacggcgcat
    14341 caggcgcgca cgctctttca tcggcagatc agcccatttc gggaacgcct ctttcgctgc
    14401 cgctaccgcc tgattgatct ccgcttcacc gccagaggcc acatccgcca gcacttcacc
    14461 cgttgccgga ttggtagtct ggaagtagtc gttacctgca acatttttgc cgttgatcca
    14521 atgatttact tttttcattt cgctgtttcc tcactcacaa ttcggttcac caggcggccc
    14581 acgccttcta cttccaccac tacttcatcg ccaggcacca cgtcagataa gccttttggt
    14641 gtgccggtgg cgatcatgtc gcccggattc agggtcataa attcgcttaa gtaggcgatc
    14701 aggaagggca cgctgaagat cagatcggcg gtggtgcctt gctggcgtaa ctcgccgttg
    14761 acgaaggtgc gaagggtcag attatgcggg tccgggatcg cctctttcgg cacgatggtt
    14821 gaaagcatcg gcgtcagtcc gtcgcggctt ttgacccgca ggttagggcg gtagtagttt
    14881 tccagatagt cgcgaatggc gtagtcgtta cacacggtgt agcccgcgac ataatccatg
    14941 gcatcggctt cgctgacgtt acgcgcctgc ttgccaataa ctaccaccag ctccgcttcg
    15001 tagtgcatgt attcaatatt gttcgggcgc acggaggtct ggttgtcgcc agtgagggta
    15061 ttcggcgctt tcaggaacac cagcggctct tccggtggct taaattccag ttcgctggcg
    15121 tggtcggcgt agttcaggcc gagggcaaac agcgtaccgt gcgggtgtgg cagcgttggg
    15181 aagctcttgc gcgtggtcac ttcacgttcg tccactaccg gattttccag cggcgggaaa
    15241 ccttctgcga gaacacgcac gcgatcgctt ggctgtattt ccacgcgcgc ctgtggcgtg
    15301 ccgagcagaa tagcatcgcc tggattcagc gtggcaaatt cgctcagggc gctcagcagc
    15361 tgtgcggcgt tacgttgtaa atcggcggta ttccagtgat cggcaggacg cccgttgatc
    15421 tcggtataga tagtcagatt atcgacattg ctgagagcca cggtttcgcc aatggggcag
    15481 aatccatcac gacattttgc tttgattgcc ggacggtaaa agctctcttc cggcaggctg
    15541 acgtcgttat ccagcgcata tccggcgatg tactctgccg catcttcttc acgtactttc
    15601 gtcgccgttt tccccacaat cagcgcaaca gtcgcaccgc tcagtacttt ttcaccctgt
    15661 ggaaagggaa tcggttcacc gcaaccaatc accgtattgc gcggtttaat aaaccagacc
    15721 gcagttttag gcggggcttt gtagggagat tgctggaacg cttcctgcca ggcatcaagt
    15781 tggctgcgat ggttcaacgc tacggcgaag atagtgcctt tcattaccac tcctcggatt
    15841 cgatcatctt aatcattaat atgt
//
LOCUS       DAZOZX010000088        15669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZOZX010000088 DAZOZX010000000
VERSION     DAZOZX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.088"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            188..430
                     /gene="yjbE"
                     /locus_tag="ACHUPT_002759"
     CDS             188..430
                     /gene="yjbE"
                     /locus_tag="ACHUPT_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0820279.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            544..1182
                     /locus_tag="ACHUPT_002760"
     CDS             544..1182
                     /locus_tag="ACHUPT_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0820280.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1179..1916
                     /locus_tag="ACHUPT_002761"
     CDS             1179..1916
                     /locus_tag="ACHUPT_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0820281.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1916..4012
                     /locus_tag="ACHUPT_002762"
     CDS             1916..4012
                     /locus_tag="ACHUPT_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0820282.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4059..4337)
                     /locus_tag="ACHUPT_002763"
     CDS             complement(4059..4337)
                     /locus_tag="ACHUPT_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820283.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4338..4481
                     /locus_tag="ACHUPT_002764"
     CDS             4338..4481
                     /locus_tag="ACHUPT_002764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820284.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4607..5017
                     /gene="psiE"
                     /locus_tag="ACHUPT_002765"
     CDS             4607..5017
                     /gene="psiE"
                     /locus_tag="ACHUPT_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0820285.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5061..6536)
                     /gene="xylE"
                     /locus_tag="ACHUPT_002766"
     CDS             complement(5061..6536)
                     /gene="xylE"
                     /locus_tag="ACHUPT_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0820286.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6907..7797)
                     /gene="malG"
                     /locus_tag="ACHUPT_002767"
     CDS             complement(6907..7797)
                     /gene="malG"
                     /locus_tag="ACHUPT_002767"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0820287.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7812..9356)
                     /gene="malF"
                     /locus_tag="ACHUPT_002768"
     CDS             complement(7812..9356)
                     /gene="malF"
                     /locus_tag="ACHUPT_002768"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR0820288.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9510..10700)
                     /gene="malE"
                     /locus_tag="ACHUPT_002769"
     CDS             complement(9510..10700)
                     /gene="malE"
                     /locus_tag="ACHUPT_002769"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0820289.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11065..12180
                     /gene="malK"
                     /locus_tag="ACHUPT_002770"
     CDS             11065..12180
                     /gene="malK"
                     /locus_tag="ACHUPT_002770"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0820290.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12252..13592
                     /gene="lamB"
                     /locus_tag="ACHUPT_002771"
     CDS             12252..13592
                     /gene="lamB"
                     /locus_tag="ACHUPT_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0820291.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13744..14664
                     /gene="malM"
                     /locus_tag="ACHUPT_002772"
     CDS             13744..14664
                     /gene="malM"
                     /locus_tag="ACHUPT_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0820292.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14893..15639
                     /locus_tag="ACHUPT_002773"
                     /pseudo
     CDS             14893..15639
                     /locus_tag="ACHUPT_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3955 a         3935 c         4038 g         3741 t
ORIGIN      
        1 ttaagcttat gatttttatt gttatttaaa tataagttga aacttatatt tgatattcat
       61 tccaattatc ctaaaactcc atcgctaatt ccccgcgccg taattcgcat gctttagttg
      121 tgtatactcg atcccgcccg aaatgttttt gggtaaatct ccattcattc aatgaaggga
      181 aattgttatg aaaaaagttc tgtatggcat ttttgccata tctgcgcttg cggcgacttc
      241 tgcgtgggct gcacctgtac aggtgggcga agcggcagag tcggcagcaa cgtcggtttc
      301 ggcggggagt tcctccgcga ccagcgtcag caccgtaagc tcggcggtgg gtgtcgcgct
      361 cgcggcaacc ggtggcggtg atggttctaa taccgggacc accacaacca cgaccaccag
      421 tacccagtaa taaagtatgt atccccaaaa taatttgagt cattgcatct gtggcttgaa
      481 gtatgaaggg atttaccata accacactcc ggtgtggtta ttctgcccct ctggagaaga
      541 gtcgtgaagc gacctgcact cattcttatc tgcctgctat tacaggcctg ttcagccacg
      601 actaaagagc tgggcaattc actgtgggac agtctgttcg gcacgccagg cgtacagctg
      661 acggacgatg atattcaaaa tatgccctac gccagccagt acatgcagct taatggcggg
      721 ccgcaattgt ttgtggtgct ggccttcgct gaagacggac aacaaaaatg ggtcactcag
      781 gatcaagcca ctcttgtcac acaacatggt cgtctggtga agactttgct tggcggcgac
      841 aacctgattg aagtgaataa ccttgccgcc gacccgctga ttaaacccgc acaaattgtt
      901 gatggtgcaa catggacgcg cacgatgggc tggaccgagt accagcaggt acgctacgcc
      961 accgcacgct cagtcttcaa atgggatggc accgataccg tcaaggtcgg cagcgatgaa
     1021 accctggttc gcgtgctgga cgaagaagtc tccaccgacc aggcgcgctg gcataaccgc
     1081 tactggatcg acagcgaagg gcaaattcgc cagtcggaac agtatctcgg cgcggattat
     1141 ttcccggtga aaaccactct catcaaggcg gcaaaacaat gattaaacaa actattgtcg
     1201 cgttgctttt gagtgtggga gcgtcatcgg tcttcgcggc aggaaccgtc aaggtgttca
     1261 gcaatggcag cagtgaggcc aaaacgctaa cgggcgcaga gcatttaatc gatctggtag
     1321 ggcaacctcg actggcaaac agctggtggc ctggtgcggt gattagcgaa gagctggcaa
     1381 cggcggcagc attgcgtcag cagcaggcgt tgctgacacg gctggcagaa cagggcgcag
     1441 attccagcac cgacgatgcc gccgccatta acgccttacg ccagcaaatc caggcattga
     1501 aagtgacagg caggcaaaaa atcaatcttg atcccgatat cgtccgcgtt gccgaacgcg
     1561 gtaacccgcc gttgcagggc aactacacgc tgtgggtcgg accaccgccg tccacggtca
     1621 cgttgttcgg gcttatcagc catcctggca atcagccatt cactccaggt cgcgacgtgg
     1681 cgagctatct ctctgaccag agcctgctca gcggtgcgga tcgcagctac gcgtgggtcg
     1741 tttacccgga cggacgcacg caaaaagcgc cggtggctta ctggaacaag cgtcacgtag
     1801 agccgatgcc cggcagcatt atttatgttg gcctcgcaga ttccgtctgg agtgagacgc
     1861 ctgatgccct taacgccgac attcttcaga ctctgacgca gcggataccc caataatgaa
     1921 aaaaagacat ctgcttagct tactggcgct gggcattagc acagcttgct acggcgaaac
     1981 atatcctgcg cccattggtc cgtcgcagtc ggatttcggt ggcgtaggat tattacaaac
     2041 tcccaccgcg cgtatggcac gggaagggga gttgagtctg aactatcgcg ataacgatca
     2101 gtaccgttat tactcagctt cagtgcaact cttcccgtgg ctggaaacaa cgctgcgcta
     2161 caccgacgtg cgcacccggc agtacagcag cgtcgaagcg ttctctggcg atcaaacgta
     2221 taaagataaa gccttcgatc tcaaactgcg tttgtgggaa gagagttact ggctgccgca
     2281 agtggcggtt ggtgcgcggg atatcggcgg tacggggctg tttgatgcgg aatatcttgt
     2341 tgccagcaaa gcctgggggc cgttcgattt tacgctcggc ctgggctggg gatatctggg
     2401 caccagcggt aatgtaaaaa atccgctctg ttcagccagt gataaatatt gctatcgcga
     2461 taacagctac aaacaggcgg gatctatcga cggtagccag atgttccacg gtcctgcctc
     2521 actgtttggc ggcgtggaat accagacgcc ctggcaaccg ctgcgtctga aactggagta
     2581 tgaaggtaat aattatcagc aggattttgc cgggaagctg gagcaaaaaa gtaagtttaa
     2641 cgtcggggcg atttatcgcg ttaccgattg ggccgacgtt aaccttagct atgaacgtgg
     2701 caacaccttt atgtttggcg tcacgctgcg caccaacttt aacgatctgc gcccgtccta
     2761 catcgataac gcccgcccgc aatatcaacc gcagccgcag gatgccattt tgcagcattc
     2821 ggtggtggcg aatcagttaa cgttgctgaa atacaacgcc ggacttgccg atccgcagat
     2881 ccaggcgaaa ggcgatacgc tgtatgttac cggcgagcag gtgaaatatc gtgattcgcg
     2941 cgaagggatc atccgtgcta atcggatcgt gatgaacgat ctgccggatg ggatcaaaac
     3001 gatccgcatt acggaaaatc gccttaacat gccgcaagtg acgacggaaa ccgatgtcgc
     3061 cagcctgaaa aatcatctcg ccggagagcc gttgggccac gaaacgacgc tggcgcacaa
     3121 acgcgtcgag ccagtggttc cgaagtccac cgagcagggc tggtatatcg acaaatcacg
     3181 ctttgatttc catatcgatc cggtgctgaa ccagtcggtc ggtggcccgg aaaactttta
     3241 catgtatcag ctgggcgtga tgggaacggc agatttgtgg ctgacggacc atctactgac
     3301 caccggcagc ctgtttgcaa atcttgccaa caactacgac aagtttaact acaccaatcc
     3361 accgcaggac tcgcacttac cgcgcgttcg tacccatgtg cgcgagtatg tgcagaacga
     3421 tgtctatgtg aataacctgc aagccaacta cttccagcat ctgggcaacg gcttctacgg
     3481 tcaggtctat ggaggttatc tcgaaaccat gtttggcggt gcgggggccg aagtgttgta
     3541 tcgtccgctg gacagtaact gggcgtttgg tctggatgcc aactacgtta aacagcgcga
     3601 ctggcgtagt gcaaaagata tgatgaaatt caccgactac agcgtgaaaa ccggacatct
     3661 gaccgcctac tggacgccat ctttcgccca ggacgtgttg gtgaaagcca gcgtcgggca
     3721 gtatctggca ggggataaag gcggcacgct ggagatcgct aaacgctttg acagcggcgt
     3781 ggtggtgggt ggctatgcca cgatcaccaa cgtttcgaaa gaggagtacg gcgaagggga
     3841 cttcaccaaa ggcgtgtatg tctcggtacc gttggatctc ttctcgtctg gcccgacacg
     3901 cagccgtgcg gcgattggct ggacgccgct gacgcgtgac ggtggtcagc aacttgggcg
     3961 taagttccag ttgtatgaca tgaccagcga ccgtagcgta aatttccgct aagtcatggg
     4021 aaaggtgcca gttttcgcac tcactggcac cctcattctt aatgacctgt tgagccatca
     4081 ggaacgcagt tcacccccgg tttacgatct ggtgtgtaaa ggcgaaaaca ttctgtcttc
     4141 ctgtccgtcg atatcggttg caggtctggt tgcctgagcg actgggccat cattgggctg
     4201 gagacattgc tttgcggtag gtcggcttta tcagcagtac cgagcggacc agcataagca
     4261 ggaagaacag agactgataa catcaaagca gcaaaataag gcttcatttt taccaccttt
     4321 atcaggttac gtttcatttg ttccagagga acattgtcga tttttcgcgc attgctggtg
     4381 gctgggaatc acctgaatgg gtgatttttg aattaccggc tttggtgcgg tttgtcttgc
     4441 cggatgcgcc gccaggcgcg gcttatccgg cctacgggta ggtatatccg gctttggtgg
     4501 aggcgcgctc caaatccagg ttgaacaaaa catacacaaa aaatatagat ctccgtcaca
     4561 tttttgcgtt atacaggaag ctcgccactg tgaaggaggt actgctatga cgtcactctc
     4621 tcgtccgcgc gtggagttta tctccaccat tttgcagacc gtactcaatc ttggcctgct
     4681 gtgcctcggc ctgattttgg ttgtcttcct cggcaaagaa acggtgcatc tggctgatgt
     4741 gctgttcgcg ccagaacaaa ccagcaaata tgagctggta gaaggactgg tggtttactt
     4801 tctctatttc gaatttatcg cgctgattgt gaagtacttt cagtccggtt ttcacttccc
     4861 gctacgttac tttgtctata ttgggatcac cgccattgtg cgcttgatca tcgtcgatca
     4921 taaatcgcca ctggatgtgt tgatctactc ggctgcgatc ctgttactgg tgatcaccct
     4981 gtggctgtgt aattcgaaac ggctgaagcg ggagtaaaaa gacagcacgc cgaaatggcg
     5041 cggcgtgctg gacaggaaga ttacagcgta gcagtttgtt gtgttttctt cgtttccggt
     5101 tcccagagcg cttccagctc ctcaagggtt ttacctttgg tttccgggac aaatttccac
     5161 ataaacagtg ctgccagaac gcccatacaa ccgtaaatcc agtaggagaa accgttgtgg
     5221 aaatgggcca ccagccagga gtttttgtcc atcatcggga aggtccagga gacgaagtag
     5281 ttcgccagcc actgggccgc caccgcgatt gccagcgctt taccacgaat agcattcggg
     5341 aagatttccg acagcagtac ccagcatacc ggaccccagg acatggcaaa ggcggcaaca
     5401 tagaacagca tcgacagtag cgccacaata cccgatgcct gagtgtaaaa cgcggtaccg
     5461 aggctaaaca taccgattgc cattccgagt gcgccgataa tttgcagtgg cttacgacca
     5521 aatttatcca cagtcataat tgccagtacg gtaaaggtga ggttgataac tccgacaata
     5581 atggtctgca acagcgcgat atccgtgctg gcccccagcg ttttgaacac ttccggcgcg
     5641 tagtacagca ccacattgat gccgacaaat tgctggaaga tggagagcat aacgccgatt
     5701 acaatcacgc ccacgccaaa catcagcaga cgaccaccgg ttttgcggcc atgatccagg
     5761 gagtgtttaa tttcctgtac tgcctgagtt gcaagcgtgt tgcccataat tttgcgcagg
     5821 atactttccg tctgttcttg cttgccgcgt gacatcagcc agcgaggact ttctggcacg
     5881 gtatacagca gcattaagaa cagcagtgca gggatacatt ccgaggcaaa catataacgc
     5941 ctgccgtcag tattcagcca gctggcatca ccggaacggg caataaaata gtttacgcag
     6001 taaactaaaa gttgcccgaa aataatcgca aactggttaa atgaaaccag ttttccgcga
     6061 atatgagctg gagccagttc cgcaatatac attggtgaaa gcattgaggc taaaccaacg
     6121 ccaataccgc caataatacg ataaataaca aattccggga cataacctgc cagataaata
     6181 ggtactgtgt tgtccgggtt tatagaggta aaaccaagtt ctggccaggc agaacctaca
     6241 ccagaaataa aaaacaggac agcagaaatc ttaagtgaat cacgacggcc gaagcggtta
     6301 ctgcaataac caccgagggc accgccgatg atgcaaccaa tcagagcgct ggctacgcaa
     6361 aatcctaaca gggagttggc agcggattca cttaagtttt gtggagcaac aaagacggta
     6421 ttgagtgact caacagtacc ggaaataacg gcggtgtcgt agccaaataa taaaccacct
     6481 aatgtagcga ctaaggtaat cgaaaatata taactggaat tatactgggt attcattcag
     6541 acctgcctta gaccattctg atcttttatc agtgcattga tgttggaatt gtcaggcagt
     6601 taaggcaatt atcatttttt acaatgcgtt tacgtaatgt cttttctgtg atcttaattg
     6661 tgataattat caaaaattga tgaaacaata ctattgccgt gactcagagc acgaaagaga
     6721 attatcgtaa gtgggaaaac aaataacgta aaaataataa gttctatgat gggaatatta
     6781 accggcgaac gattcagatt gcagacgaaa gaaaaaaagg cgctccgtgg agcgccgaat
     6841 aatagtcaca agttgggata acgtaagttg agggtgcagc ggcataacat tggcagaaca
     6901 acatctttaa cctttcacac cacctgccgt caggccgttg accagccagc gttgagccag
     6961 caagaagacg atggtgatcg gtaatgcaga catcacggca gcggcggcaa agtcacccca
     7021 caggtagttt tgcgggttga ggtattgctg catccccacg gccagggtgt agctgtttac
     7081 gtcacgcagt aacagcgacg cgaccggaac ttcagtaatg gcagcgataa acgacaggat
     7141 gaataccacc gccagaatcg gtactgacag cggcaacagg acaaggcgga aggcctgcca
     7201 cggtgtcgca ccatccagcg cagccgcttc ttccagcgaa ctgtcgatgg tttcgaaata
     7261 gcctttgatg gtccagacat gcagcgcaat cccacccaga tacgcgaaaa ttacgccgcc
     7321 gtgagtattc aggccaatga atggaatgta ctcacccaga cgatcaaaca acgcatacaa
     7381 cgcgaccagt gaaagtactg ccgggaacat ctggaaaatc agcattcctt tcagcagcgt
     7441 cgctttgcct ggaaagcgca tacgggcgaa agcgtaggcg caggtggtgg agagcgccac
     7501 aatgccaatc gcggaaatcc cagcgacctt taccgagttc cacagccaca gcagtaccgg
     7561 gaatggcggt ggcgtaatgc gaccatcagc ctgttcaacg ctaaaaccta acgccagttt
     7621 ccagtgatcc caggagattt gctccgggat caggctaccg gtcgcgaagt ttccctgacg
     7681 cagcgagata gcgacgacca tcagcagcgg gaacataatc gccgcgataa aaagtagcag
     7741 tagcaggtga gtaataaaca aacgtgcttt ttgcgatttc ggttggacca ttgccatttt
     7801 tgttatctcc cttaatcaaa cttcattcgc gtggctttca ggttcactat cgccagcgca
     7861 cccaccagca ggaagatcag cgtggcaatt gctgccgcca gaccgaagtc ctgacccccg
     7921 ccgccttcaa aagcgatacg gtaggtgtag ttaacaagca ggtcggtgta accggctggc
     7981 gtggtcgtgc caagacgatc cgggccgccg ttggttaaca gctgaatcag cacgaagttg
     8041 ttaaagttaa aggcgaagct ggcgatcatc agcggcgtca gcggtttaat cagcagcggc
     8101 agcgtaatct taaagaagtt ctggaacgga cctgcgccat ccattgctga ggcttcatac
     8161 aaatcgtccg gaatcgcttt cagcaagccc atgcagagga tcatcatgta cggataaccc
     8221 agccaggtat tgacgataat cagcatcgtg cgagcggtgg tcggatcgct gaaccaggcg
     8281 ggcttcacgc caaacagcgc gctcaacatc atgttgattt caccgaagct ctggttaaac
     8341 aatcctttga aaatcaagat tgaaatgaac gatggcaccg cgtagggcag aatcagcagg
     8401 acgcgataga ccgctttgcc gcgcaacgct tcccactgca ccagacatgc cagaaccatg
     8461 ccgaccgcca ccgttaaaaa gacagtgatc agcgagaaca ccacggtcca gacgaaaatg
     8521 gcgaggaacg gtttctgaat gccttcgtcg gtaaagacgc gggtaaagtt tttccagccg
     8581 gtggtcacgg tgtaaccggg gcttagcttt tcatcccccc agttgccgtc ggcggtaatg
     8641 gactggtaaa agccaatttg gttattcgga cgatatttca cgccactctg attattcgtc
     8701 aacgtgccgt caccgtcgag tgtgtagagc ggctgcgtgc cagaaaactg gcgcagggag
     8761 ctcatcatca ctttgttgcc atccggcaga atggcggtaa tgtcactcag cgcctgacga
     8821 ttctgggtaa tcacgcgcag attcgcgcgt tcgccttcgg gctgggcggt cgtttctttc
     8881 agttgcagtt tttgctcgcc gccaaattta aacgcgtcgg agaggtaatt tttgccggtt
     8941 tcgccgtcgc tgagcgccag ttgccactca tcgcccgccg ggtaaagacc aaagttatag
     9001 gttttgcctg cttgccagga gcgatctaac aacacttcct gcgcacgttc aaaagtcagc
     9061 tggttagtgc tgctgtagtt ggtgaaggca atggcgatgg tgcagaccag agggaagagg
     9121 acgaataatc ccattccagc cattcccggg taaacatagc gccaggcgta ggctttacga
     9181 ttggcgaaaa tatacagccc cgctgaactc aatatcagcg tggtaatggc gaacaggtat
     9241 tccccttgtg cgtacattaa aacaacaagg taacccacca gcaggccgag cagacctagc
     9301 actgaccatt tcagcgcgtc gctttgccac caatgtttct ttttaatgac atccatgggg
     9361 ttcttcctca ttccaggacg gataaggctt tcacgcctta tccgacaaca actgcctgat
     9421 gcgacactga tgcgtcttat caggcctaca tgcgtttcag ttttgtaggc cggacaaggc
     9481 gttcacgccg catccggcat ttcacagcat tacttggtga tacgagtctg cgcgtctttc
     9541 agggcttcat cgacagtctg acgaccgctg gcggcgttga tcaccgcagt acgtacggca
     9601 taccagaaag cggacatctg cgggatgttc ggcatgattt cacctttctg ggcgttttcc
     9661 atggtggcgg caatacgtgg atctttcgcc aactcttcct cgtaagactt cagcgctacg
     9721 gcacccagcg gtttgtcttt attaaccgct tccagacctt catcagtcag cagatagttt
     9781 tcgaggaact ctttcgccag ctctttgttc ggactggcgg cgttaatacc ggtgctcagc
     9841 acgccaacga acggtttaga tggttgaccc ttgaaggtcg gcagtaccgt tacaccataa
     9901 ttcactttgc tggtgtcgat gttggaccat gcccacgggc cgttgatggt catcgctgtt
     9961 tcgcctttat taaaggcagc ttctgcgatg gagtaatcgg tgtctgcatt catgtgtttg
    10021 tttttaatca ggtcaaccag gaaggtcaga cccgctttcg cgccagcgtt atccacgccc
    10081 acgtctttaa tgtcgtactt gccgttttca tacttgaacg cataaccccc gtcagcagca
    10141 atcagcggcc aggtgaagta cggttcttgc aggttgaaca tcagcgcgct cttacctttc
    10201 gctttcagtt ctttatccag cgccgggatc tcttcccagg tttttggcgg gttcggcagc
    10261 aggtctttgt tataaatcag cgataacgct tcaacagcga ttgggtaagc aatcagcttg
    10321 ccgttgtaac gtacggcatc ccatgtaaac ggatacagct tgtcctggaa cgctttgtcc
    10381 ggggtgattt cagccaacag gccagattga gcgtagccac caaagcggtc gtgcgcccag
    10441 aagataatgt cagggccatc gccagttgcc gcaacctgag ggaatttctc ttccagttta
    10501 tccggatgct caacggtgac tttaattccg gtatctttct cgaatttctt accgacttca
    10561 gcgagaccgt tatagccttt atcgccgtta atccagatta ccagtttacc ttcttcgatt
    10621 ttggcgagag ccgaggcgga aaacatcatc gtcgttaatg cggataatgc gaggatgcgt
    10681 gcacctgttt ttattttcat aatctatggt ccttgttggt gaagtgctcg tgaaaacacc
    10741 taaacggact ctagtttctt tatacggcaa cctctttcca tcctccttgc ccctacgccc
    10801 caccgttgct ttgtgtgatc tctgttacag aattggcggt aatgtgggga tgcgcacata
    10861 aaatcgccac gatttttgca agcaacatca cgaaattcct tacatgacct cggtttagtt
    10921 cacagaagcc gcgttctcat cctcccgcct cctcccccat aaaaaagcca gggggtggag
    10981 gatttaagcc atctcctgat gacgcatagt cagcccatca tgaatgttgc tgtcgatgac
    11041 aggttgttac aaagggagaa gggcatggcg agcgtacagc tgcaaaatgt aacgaaagcc
    11101 tggggcgagg tcgtggtatc gaaagatatc aatctcgata tccatgaagg tgaattcgtg
    11161 gtgtttgtcg gaccgtctgg ctgcggtaaa tcgactttac tgcgcatgat tgccgggctt
    11221 gagacgatca ccagcggcga cctgttcatc ggtgagaaac ggatgaatga cactccgcca
    11281 gcagaacgcg gcgttggtat ggtgtttcag tcttacgcgc tctatcccca cctgtcagta
    11341 gcagaaaaca tgtcatttgg cctgaaactg gctggcgcaa aaaaagaggt gattaaccaa
    11401 cgcgtcaacc aggtggcgga agtgctgcaa ctggcgcatt tgctggatcg caaaccgaaa
    11461 gcgctctccg gtggtcagcg tcagcgcgtg gcgattggcc gtacgctggt ggccgagcca
    11521 agcgtatttt tgctcgatga accgctctcc aacctcgatg ctgcactgcg tgtgcaaatg
    11581 cgtatcgaaa tctcccgtct gcataaacgc ctgggccgca caatgattta cgtcacccac
    11641 gatcaggtcg aagcgatgac gctggccgac aaaatcgtgg tgctggacgc cggtcgcgtg
    11701 gcacaggttg ggaaaccgct ggaactgtac cactatccgg cagaccgttt tgtcgccgga
    11761 tttatcggtt cgccaaagat gaacttcctg ccggtaaaag tgaccgccac cgcaatcgat
    11821 caagtgcagg tggagctgcc gatgccaaat cgtcaacaag tctggctgcc tgttgaaagc
    11881 cgtgatgtcc aggttggagc caatatgtcg ctgggtattc gcccggaaca tctactgccg
    11941 agtgatatcg ctgacgtcat ccttgagggt gaagttcagg tcgtcgagca actcggcaac
    12001 gaaacccaaa tctatatcca gatcccttcc attcgtcaaa acctggtgta ccgccagaac
    12061 gacgtggtgt tggtagaaga aggtgccaca ttcgctatcg gcctgccgcc agagcgttgc
    12121 catctgttcc gtgaggatgg cactgcatgt cgtcgactgc ataaggagcc gggcgtttaa
    12181 gcaccccaca aaacacacaa agcctgtcac aggtgatgtg aaaaaagaaa agcaatgact
    12241 caggagatag aatgatgatt actctgcgca aacttcctct ggcggttgcc gtcgcagcgg
    12301 gcgtaatgtc tgctcaggca atggctgttg atttccacgg ctatgcacgt tccggtattg
    12361 gctggacagg tagcggcggt gaacaacagt gtttccagac taccggtgct caaagtaaat
    12421 accgtcttgg caacgaatgt gaaacttatg ctgaattaaa attgggtcag gaagtgtgga
    12481 aagagggcga taagagcttc tatttcgaca ctaacgtggc ctattccgtc gcgcaacaga
    12541 atgactggga agctaccgat ccggccttcc gtgaagcaaa cgtgcaggga aaaaacctga
    12601 tcgaatggct gccaggttcc accatctggg caggtaagcg cttctaccaa cgtcatgacg
    12661 ttcatatgat cgacttctac tactgggata tttctggtcc tggtgccggt ctggaaaaca
    12721 tcgatgttgg cttcggtaaa ctctctctgg cagcaacccg ctcctctgaa gctggtggtt
    12781 cttcctcttt tgccagcaac aatatttatg actataccaa cgaaaccgcg aacgacgttt
    12841 tcgatgtgcg tttagcgcag atggaaatca acccgggcgg cacattagaa ctgggtgtcg
    12901 actacggtcg tgccaacctg cgtgataact atcgtctggt tgatggcgca tcgaaagacg
    12961 gctggttatt cactgctgaa catactcaga gtgtcctgaa gggctttaac aagtttgttg
    13021 ttcagtacgc tactgactcg atgacctcgc agggtaaagg tctgtcgcag ggttctggcg
    13081 tcgcgtttga taacgaaaaa tttgcctaca atatcaacaa caacggtcac atgctgcgta
    13141 tcctcgacca cggtgcgatc tccatgggcg acaactggga catgatgtac gtgggtatgt
    13201 accaggatat caactgggat aacgacaacg gcaccaagtg gtggaccgtt ggtattcgcc
    13261 cgatgtacaa gtggacgcca atcatgagca ccgtgatgga aatcggctac gacaacgtcg
    13321 aatcccagcg caccggcgac aagaacaatc agtacaaaat taccctcgca caacaatggc
    13381 aggctggcga cagcatctgg tcacgcccgg ctattcgtgt cttcgcaacc tacgccaagt
    13441 gggatgagaa atggggttac gactacaacg gcgatagcaa ggttaacccg aactacggca
    13501 aagccgttcc tgctgatttc aacggcggca gcttcggtcg tggcgacagc gacgagtgga
    13561 ccttcggtgc ccagatggaa atctggtggt aatagcaaaa cctgggccgg ataaggcgtt
    13621 tacgccgcat ccggcataaa aacaggttgt cattatctga aaggggcgaa agcccctctg
    13681 attatcgggt ttagcgcgct attgcctggc taccgctgag ctccagattt tgaggtgaaa
    13741 acaatgaaaa tgaataaaag tctcatcgcc ctctgtttat cagcagggtt actggcaagc
    13801 gcgcctggaa ttagccttgc cgatgttaac tacgtaccgc aaaacaccag cgacgcgcca
    13861 gccattccat ctgccgcgct gcaacaactc acctggacac cggtcgatca atctaaaacc
    13921 cagaccaccc aactggcgac cggcggccaa caactgaacg ttcccggcat cagtggtccg
    13981 gttgctgcgt acagcgtccc ggcaaacatt ggcgaactga ccctgacgct gaccagcgaa
    14041 gtgaacaaac aaaccagcgt ttttgcgccg aacgtgctga ttcttgatca gaacatgacc
    14101 ccatcagcct tcttccccag cagttatttc acctaccagg aactaggcgt gatgagtgca
    14161 gatcggctgg aaggcgttat gcgcctgaca ccggcgttgg ggcagcaaaa actttatgtt
    14221 ctggtcttta ccacggaaaa agatctccag cagacgaccc aactgctcga cccggctaaa
    14281 gcctatgcca aaggcgtcgg taactcgatc ccggatattc ccgatccggt tgctcgtcat
    14341 accagcgatg gcttactgaa actgaaagtc aaaacgaact ccagctccag cgtgttggta
    14401 ggaccgctat ttggttcttc cgctccagct ccggttacgg taggcaacac ggcggcacca
    14461 gctgtggctg cacctgctcc ggtgccggtg aagaaaagcg agccgatgct caacgacacg
    14521 gaaagttatt ttaataccgc gatcaaaaac gctgtcgcga aaggtgatgt tgataaggcg
    14581 ttaaaactgc ttgatgaagc tgaacgcctg ggatcgacat ctgcccgttc cacctttatc
    14641 agcagtgtaa aaggcaaggg gtaattacgc cccacagtgc tgattttgca acaactggtg
    14701 cgtctcctgg cgcacctttt tttatgcttc cttcctggga tatgagcgat tttttatagt
    14761 acttcacttc ttcttcacta agaatgtcca ttatttcaat gcattatcag agattctttt
    14821 cctttcgccg gtagtgtctg gacgctcagg ctacttttcc aggttatttt atttctgtat
    14881 tacagaggtt ttatgatagg tcatatcaca aattctggcg gcaataactc tttgatgaaa
    14941 catgatgtgg tgcaaggaaa taatatagta gatcttgatt tactacgtaa tttcaatggg
    15001 gtgacaggtt taaacagaga ttactttatt tatatcagtg atatattttt aaatataaaa
    15061 caacggaacg aaaaaaatca tgcaataaat atgttacgtg aagtctcaat tagtaatgat
    15121 actataagtg taaaattcta cagaaatgaa aaaatagaat gcgcttgtaa ttttctgatg
    15181 gataaagatg cgcaggggta tatcgacctg tctgattttg atttaacaaa ttgtcatttt
    15241 aaaggtgacg ttatttctaa ggtgtctttt ttatcatcaa atctacaaca tgtaactttc
    15301 gaatgtaaag aaattgagaa ttgcaatttt actaaggcaa cagttgataa tgtcatattt
    15361 aaatgtcgac gtttacacaa tgtgattttt ctcaaaacga gtggcgaatg tgttgatttt
    15421 agccaaaata ttcttgatac agttgacttc tcgcagagtc aacttggtca tagtaatttt
    15481 cgcgaatgtc agattagaaa ttcaaacttc gataattgtt atctttacgc ttcgcacttc
    15541 accagagcag agtttctgtc tgccaaagaa atatcattta ttaaatcgaa tttgacagct
    15601 gttatgtttg atcatgtgcg aatgtcgaca gggaattttt aagattgcat tacagaacaa
    15661 tttgaatta
//
LOCUS       DAZOZX010000089        15663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZOZX010000089 DAZOZX010000000
VERSION     DAZOZX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.089"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            208..1266
                     /gene="citC"
                     /locus_tag="ACHUPT_002774"
     CDS             208..1266
                     /gene="citC"
                     /locus_tag="ACHUPT_002774"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0820293.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1281..1577
                     /gene="citD"
                     /locus_tag="ACHUPT_002775"
     CDS             1281..1577
                     /gene="citD"
                     /locus_tag="ACHUPT_002775"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0820294.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1574..2482
                     /gene="citE"
                     /locus_tag="ACHUPT_002776"
     CDS             1574..2482
                     /gene="citE"
                     /locus_tag="ACHUPT_002776"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0820295.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2493..4025
                     /gene="citF"
                     /locus_tag="ACHUPT_002777"
     CDS             2493..4025
                     /gene="citF"
                     /locus_tag="ACHUPT_002777"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR0820296.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4029..4579
                     /gene="citX"
                     /locus_tag="ACHUPT_002778"
                     /pseudo
     CDS             4029..4579
                     /gene="citX"
                     /locus_tag="ACHUPT_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4554..5432
                     /gene="citG"
                     /locus_tag="ACHUPT_002779"
     CDS             4554..5432
                     /gene="citG"
                     /locus_tag="ACHUPT_002779"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0820297.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5483..6945
                     /gene="citT"
                     /locus_tag="ACHUPT_002780"
                     /pseudo
     CDS             5483..6945
                     /gene="citT"
                     /locus_tag="ACHUPT_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7059..7865
                     /gene="rna"
                     /locus_tag="ACHUPT_002781"
     CDS             7059..7865
                     /gene="rna"
                     /locus_tag="ACHUPT_002781"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0820298.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8094..8504
                     /gene="rnk"
                     /locus_tag="ACHUPT_002782"
     CDS             8094..8504
                     /gene="rnk"
                     /locus_tag="ACHUPT_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0820299.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8642..8731
                     /gene="yldA"
                     /locus_tag="ACHUPT_002783"
     CDS             8642..8731
                     /gene="yldA"
                     /locus_tag="ACHUPT_002783"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0820300.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8735..9944)
                     /locus_tag="ACHUPT_002784"
                     /pseudo
     CDS             complement(8735..9944)
                     /locus_tag="ACHUPT_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10165..10593
                     /gene="uspG"
                     /locus_tag="ACHUPT_002785"
     CDS             10165..10593
                     /gene="uspG"
                     /locus_tag="ACHUPT_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0820301.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10714..12279)
                     /gene="ahpF"
                     /locus_tag="ACHUPT_002786"
     CDS             complement(10714..12279)
                     /gene="ahpF"
                     /locus_tag="ACHUPT_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0820302.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12524..13087)
                     /gene="ahpC"
                     /locus_tag="ACHUPT_002787"
     CDS             complement(12524..13087)
                     /gene="ahpC"
                     /locus_tag="ACHUPT_002787"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0820303.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13459..14205
                     /gene="dsbG"
                     /locus_tag="ACHUPT_002788"
     CDS             13459..14205
                     /gene="dsbG"
                     /locus_tag="ACHUPT_002788"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR0820304.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14415..15317
                     /locus_tag="ACHUPT_002789"
     CDS             14415..15317
                     /locus_tag="ACHUPT_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0820305.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15464..15663
                     /locus_tag="ACHUPT_002790"
                     /pseudo
     CDS             15464..15663
                     /locus_tag="ACHUPT_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3867 a         4064 c         3810 g         3922 t
ORIGIN      
        1 aaataaataa aaaaccacta actctatgtg aaataaatca aaatttcacg ccgaaatact
       61 ccttaggatg tatagcaaaa ggagaaaaag atatacctcg atcacccccc tttctcccaa
      121 gtgaaaataa aaggttatca gtttgcaaca ttgaacaaca ttcgttgcaa atcgataaca
      181 acatgcacct tcaggatact atttattatg ttcggcaatg atattttcac ccgcgtaaaa
      241 cgttcagaaa ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa tgatttgagc
      301 gttgacacca cagtcgaagt atttattacc gtaacccgcg atgaaaagct tatcgcgtgc
      361 ggtggaattg ccggaaatat tattaaatgc gttgctatca gtgaatccgt tcgcggtgaa
      421 ggactggcgc tgacattagc cactgaactg ataaacctcg cctatgagcg gcacagcacg
      481 catctgttta tttataccaa aaccgaatac gaggcgctgt tccgccagtg cggtttttcc
      541 acgctgacct gcgtacccgg cgtgatggtg ctgatggaaa acagcgccac gcgactgaaa
      601 cgctatgccg aatcgctgaa aaaatttcgt catccaggga acaagattgg ctgcattgtg
      661 atgaacgcca atccctttac gaatggtcac cgttatctga ttcaacaagc tgcggcacag
      721 tgcgactggt tgcatctgtt tttagtcaaa gaagattctt cacgcttccc ctatgaagac
      781 cggctggatc tggtgttaaa aggcaccgcc gatattccac gcctgactgt gcatcgcggc
      841 tcagaataca tcatctcccg cgctacgttc ccttgctact tcattaaaga acagagcgtc
      901 attaaccatt gttacaccga aattgatctg aagattttcc gtcagtacct cgctcccgcg
      961 cttggtgtaa ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac cgcccagtac
     1021 aaccaggata tgcgctactg gctggaaacg ccgactatct ccgcagcgcc catcgaactg
     1081 gttgaaattg agcggctgcg ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa
     1141 ctgctggcga aaaacgatct cacggctatc gcgccgctgg tccctgcagt cacgctgcat
     1201 tatttgcaga acctgcttga gcactcccgc caggacgcgg cagctcgtca aaagaccccc
     1261 gcatgagaaa caggtgaaaa atgaaaataa accagcccgc cgttgcaggc acccttgagt
     1321 ctggggatgt gatgatacgc atcgccccac tcgatacgca ggatatcgac ctgcaaatca
     1381 atagcagcgt tgagaaacag tttggcgatg caattcgcac caccattctg gacgttctcg
     1441 cccgctacaa cgtgcgcggc gtacagctga atgtcgatga caaaggcgca ctggactgca
     1501 ttttacatgc acgactggaa gccctgctgg cacgcgccag cggtatcccg gctctgccat
     1561 gggaggattg ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag
     1621 catgttgttt gtgcctggtg ccaatgccgc gatggtcagc aactccttca tctacccggc
     1681 tgatgccctg atgtttgacc tcgaagactc cgtggcatta cgtgaaaaag acaccgcccg
     1741 ccgcatggtt tatcacgcgc tgcaacatcc gctgtatcgc gatattgaaa ccattgtgcg
     1801 tgtcaacgcg ctggattccg aatggggtgt taacgacctg gaagccgtcg ttcgcggtgg
     1861 tgcggacgtt gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg atattgaaaa
     1921 agagatcctg cgtatagaaa aagcctgtgg tcgtgaaccc ggcagcaccg gcctgctggc
     1981 ggcgattgaa tctccgctgg gcattacccg cgcagtggaa atcgctcacg cttccgagcg
     2041 tttgatcggt atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta cagaacgctc
     2101 cccggaagga actgaactgc tgttcgcacg ctgttccatt ttgcaggccg cgcgctctgc
     2161 gggtattcag gcgttcgata ccgtctattc cgacgctaac aacgaagccg gattcctgca
     2221 agaagccgcc cacatcaaac agctgggctt tgacggcaaa tcgctgatca acccgcgtca
     2281 gattgatctg ctgcacaacc tctacgcacc gacccagaaa gaagtggatc acgcccaccg
     2341 cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg
     2401 caagatggtg gacggtccgg ttatcgatcg cgcccgtctg gtgctctccc gtgcagaact
     2461 ttccggcatc cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa caatctcaac
     2521 gacaagaacg ggtagcggcc tggaatcgtc gcactgaatg cgatcttgcc gctttccaga
     2581 actcaccaaa gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg tgcgccaacc
     2641 tggaagaagc gattcgtcgc tcaggtttac aggacggcat gacggtttcc ttccatcacg
     2701 ctttccgtgg cggtgacctg accgtcaata tggtgatgga cgtcatcgcg aagatgggct
     2761 ttaaaaacct gaccctggcg tccagctccc tgagtgattg ccatgcgccg ctggtagaac
     2821 acattcgcca gggcgtggtt acccgcattt atacctctgg cctacgcggt ccactggcgg
     2881 aagagatctc ccgtggtctg ctggcggaac cggtgcagat ccactctcac ggcggtcgtg
     2941 tgcatctggt acagagcggc gaactgaata tcgacgtggc tttcctcggc gtcccgtcct
     3001 gtgatgaatt cggtaatgcc aacggctaca ccggtaaagc ctgctgcggc tccctcggct
     3061 atgcaatggt tgatgccgac aacgcaaaac aggtcgtgat gcttaccgaa gaactgctgc
     3121 cttatccgca taatccggca agcattgagc aagatcaggt tgatttgatc gtcaaagtta
     3181 atcgcgttgg cgatgctgca aaaatcggcg ctggtactac ccgtatgacc actaacccgc
     3241 gcgaactgct tattgcccgt agcgctgcgg atgtgattgt caactctggc tacttcaaag
     3301 aaggtttctc catgcaaacc ggcaccggcg gcgcttcgct ggcggtaacc cgtttcctgg
     3361 aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc cctcggcggt attaccgcga
     3421 cgatggttga tctgcacgaa aaaggtctga tccgcaaact gctggatgtg cagagctttg
     3481 acagccatgc tgcgcaatcg ctggcccgta accccaatca catcgaaatc agcgccaacc
     3541 agtacgctaa ctggggttcg aaaggcgcat cggttgatcg tctcgacgtg gtggtactga
     3601 gcgcgctgga aattgacagg cagttcaacg ttaacgtgct gaccggttct gacggcgtac
     3661 tgcgtggcgc ttccggtggt cactgcgata ccgcgattgc ctctgcgctt tccatcatcg
     3721 tcgcgccact ggtacgcggt cgtattccga ctctggtgga taacgtgctg acctgcatca
     3781 ccccgggctc cagtgtcgat attctggtca cagaccacgg tatcgcagtt aacccggcac
     3841 gtccggaact agcagaacgt ctgcaggaag cgggcattaa agtggtttcc attgagtggc
     3901 tgcgcgaacg tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc accgaccgcg
     3961 tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga tgttgtgcat caggtgaagg
     4021 aataagccat gcacctgctt cctgaactcg ccagccacca tgcggtgtca attcccgagc
     4081 tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgc ctggctcaag caccatcctg
     4141 ttccactggt ctcctttacc gtggttgcgc ctgggccgat taaagacagc gaggtcacac
     4201 gccgaatttt taatcatcgc gtgacagcct tgcgtgcctt agccacaaag cagggctggc
     4261 aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggcatg ttgagcattg
     4321 ccgccccggc tcgcgacctc aagctcgcca ccattgagct tgaacatagt catcctctcg
     4381 ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc cgccgcgact
     4441 attcactgcg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
     4501 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
     4561 tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
     4621 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg
     4681 atcgcattaa ctgcggtgcg cacaaagata tggcgctgga agatttccac cgcagcgcgc
     4741 tggcgattca gggctggtta ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg
     4801 caccggaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
     4861 tccgcgccac tgcgggcgta aacacgcata aaggcagcat tttttcttta gggctgctat
     4921 gtgcggcaat tggccgtttg cttcagctca accagccggt tacgccaaca accgtttgtt
     4981 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
     5041 gactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
     5101 ccgaagcagg ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg
     5161 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggctacca
     5221 acggcgatac caacgttgca tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
     5281 aggcgcaaac attattgcaa aaagggggca ttcgaacccc caccgatctc gattatctcc
     5341 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
     5401 tgatccttac ctgtttttta gcacagattt aattatttaa gcacttgata aatttggaaa
     5461 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
     5521 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca
     5581 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
     5641 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
     5701 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
     5761 atggggcctg gctggttttt ccagcaccac ggtatggctg gtatttggcg catttatttt
     5821 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
     5881 catggacaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
     5941 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
     6001 cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta
     6061 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc
     6121 ggaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca gattagctgg
     6181 ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat tgcgccgtgg
     6241 ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc aacctgggcg
     6301 ggtgatgaac taaaaaccat gggtgcgctg acacgcagag agtggacgct gattggcctt
     6361 gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc tactgcggtt
     6421 ggtctgctgg cagtttcgct aatgctggct ctgcacgttg tgccgtggaa agacattacc
     6481 cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt gatggctaac
     6541 ggcctgactc gttctggttt tattgactgg ttcgccggta ccatgagtac gcacttggaa
     6601 ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt tgcacactac
     6661 ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct ggccgtcggt
     6721 aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct gtctatcggt
     6781 atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta cggctgtggc
     6841 tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat ttacatctct
     6901 atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt tcgccaaacg
     6961 ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt atgatttaat
     7021 agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc tgccgtaacg
     7081 ccgcgttgct cgcggtttct ctgcttccct tatcttctgc caacgccgta gcgttgcagg
     7141 caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa accggatttt
     7201 gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa
     7261 cgaccaacaa aactgatttt ctgaccgtac atggtctgtg gccaggattg cctaaatcgg
     7321 ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct actcgcccaa
     7381 tcccgaatct accagaagca cgcgccagcc gaatgtgttc atcgccggaa accgggttat
     7441 cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga cgttcctgcc
     7501 tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg gacgcatact
     7561 tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga aaattccttg
     7621 cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt gccaaaagct
     7681 ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct gcgtatttga
     7741 ctgaaattca gatctcgatc aaagctgacg ccatcaacgc tccgctttct gcaaactcat
     7801 tcttgccaca acctcaccca ggtaactgtg gcaaaacctt tgtgattgat aaagcgggtt
     7861 attaatcctc tgatttacaa aacacgctaa ttcgtcttcc ataaccatgc cgattaagaa
     7921 ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca cttcaaagag
     7981 tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt
     8041 cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta aaaatgtcca
     8101 gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg ctggagcaac
     8161 ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc agagttggat cgcgcccaaa
     8221 tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg gttaaattcc
     8281 gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg
     8341 atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg gggctgcgcg
     8401 ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt gaagtgctgg
     8461 aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat ctgcacaagc
     8521 gataactcgc tttgcccaga ggatgcactc gcatcctctt tacgcaaaat cagcaatttt
     8581 tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg tgggaataac
     8641 catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga ttatcgtcat
     8701 ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc ggcatcgcat
     8761 tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg ggtaccagaa
     8821 tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg gcggcctctt
     8881 caaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc tcaattaacg
     8941 gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac gtcaaccctt
     9001 tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg cccggtacgc
     9061 taacaatgcc gcaacgcttt accgctgctg ccctccagtt tcaggttagt cagcaccgtt
     9121 tccgtggtgc tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg
     9181 tgacctgccg tttgttcaat aattgactgt gccggatcgc tatcttcatc gaaattaatc
     9241 gggatcgcgc cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg atcaacaaca
     9301 aaaatctgtt ccgcaccgag caaccgtgca caggcgattg tcaacaatcc cacaggacca
     9361 gcaccataga ctgcaacgct tgaaccttgt tggatctgca cattttttgc tgcctgccat
     9421 gccgttggca gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa aggcggtact
     9481 ttaaacggcc ccacattccc tttcgggacg cggacatatt ccgcctgccc gccaggaacg
     9541 ccaccataca ggtgactata accaaacaat gccgcgggag caggtatctg ttttttattg
     9601 agcgcagcgc ctttacccgc attggtattt tcgcaggcgg catattgctg taatcgacag
     9661 aaaaaacagt cgccacaagc aatgacgaac ggaattacca cccggtcgcc tttttgcaaa
     9721 ttttttacgt ccttcccggt ttcaacgact tcccccataa attcatgacc aaaaatatcg
     9781 ccatgtttaa cctggggtat tttgcctcga taaagatgga ggtcagagcc acagatcgcc
     9841 gttaccgtaa tacgcagaat aatatcatct gcctgttcaa tgcccggatc gggaacattt
     9901 tctacctgaa cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc tccagagaca
     9961 gttcaaaaaa gagtcagtct ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc
    10021 aggttttggg agaattccta gtgttcagtg agaatatatt aatcacagaa accgttcgta
    10081 aggaaaaaga accagacaaa tatcagggat aaatattata atcatggaga atgatttgtt
    10141 catgattaac agggagaaag ggttatgtat aaggcaatca ttatgccagt tgatgtattt
    10201 gaaatggaat tgagcgacaa agctgttcgc cacgctgaat tcctcgccca ggatgacgga
    10261 gttattcatc tacttcacgt actacccggg tcagccagcc tgagcctgca ccgttttgcc
    10321 gctgatgtgc gtcgttttga agagcatctg caacatgaag cagaagaacg tctgcaaacg
    10381 atggtcagcc acttcaccat cgatccttcc cgcattaaac aacatgtccg ttttggtagc
    10441 gtgcgggatg aagtcaatga gttggcagaa gaactggggg ctgatgttgt agttatcggt
    10501 tctcgcaacc catcgatttc gacccatctg ttaggttcta acgcctcgag cgtgatccgc
    10561 cacgccaatc tgcctgtact ggtcgttcgc taacagataa aagtatttat gccgccctgc
    10621 ctggaacatc ccggcagggc tttttaatgc cataaaaaag ccgccaggtt tgaccccggc
    10681 ggctaagcaa ttgcaggtga atcttacttc ttcttatgca gttttggtgc gaatcaggta
    10741 gtcaaaagca ctcagagagg ctttggcacc ttcgccagtg gcgatgatga tctgcttgta
    10801 cggaaccgtc gtacagtcac ccgctgcgaa cacgcctttg acgttggttt cacatttcgc
    10861 atcaatgata atctcgccca tgcggttacg ttcgactgcg ccttccagcc agttggtgtt
    10921 cggcagcaga ccaatctgga caaaaatacc ggccagttcg atgttgtgaa tattgccgct
    10981 gacacgatct cgatattcca gaccaacgac tttgctgccg tcgcctttca cttccgtggt
    11041 ttgcgcattc agaataatgt cgacgttttt caggctgcgc agtttgtcct gcagaacctg
    11101 gtcggctttc atttctggcg caaattccag cagcgttacg tgctcaacga tacccgccag
    11161 gtcgattgcc gcttccacgc cggagttacc gccgccgata accgctacgc gtttgccttt
    11221 aaacagcggg ccgtcgcagt gcgggcagta ggtcacgcct ttagtgcgat actgatcttc
    11281 gcccggaacg ttcatgttgc gccattttgc acccgtcgcc acgataatgc tgcgtgcttt
    11341 cagtaccgcg ccagaagctg tttcaatctg gtgtagacca ccttcaaccg ctgccgggat
    11401 cagtttgctg gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt
    11461 cagtgcgcct gccaacttct gcccttcagt cttcggtaca gaaatgtagt tttcgatatc
    11521 aacggtatcg aggatctgac caccaaaacg ttcgcccatc agaccggtac ggatgccttt
    11581 acgtgcggag taaattgctg ccgctgcacc cgccgggccg gaaccaacga ttaatacgtc
    11641 ataagcatca cgcttgttca gctcttctgc cgcacgattt tccgcgccag tatcaatttt
    11701 ggcaacgatt tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac
    11761 tgccggaacg cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat
    11821 tgcagtgtgc ttgatgcgcg ggttcaatac gctcatcagg ttcagcgcct gcaccacgtc
    11881 cgggcagtta tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg
    11941 gcgaatctgc tccagcagag actgcgcttc tttcgacgga tgaccaccgg tccacagcaa
    12001 cgccagtacc agcgaggtga actcgtggcc cagcggggag cctgcaaaac gtggtccctg
    12061 gttggaacct gggttggtga tcaggaaaga cggcttacgc actggcaagc tgttatcttc
    12121 tttaaaggtg actttgtctg acagttctgc gatttcagcc aacagttcct tgatttctgc
    12181 cgatttagcg ctgtcatcca gcgtggcaat taactcaaca ggcttggtca atttctcaag
    12241 gtaagccttg agttgagttt tcatatttgt gtcgagcatg tttatctcct gggctttaaa
    12301 catcatcatg caagcggcct ctgcggctac ctggatgcag cttgcatcat ggtgcgggga
    12361 agaaaatcgg gtgccgcagc acccgaagaa ttaggtgaat ttccgaagta tttcgcgtca
    12421 gagcaaggcg actagcctgt gaatccccgg gagcttacac aagtaagtga ccggggtgag
    12481 cgggcgacgc caacgccgct atggcgtgaa agacgaagga aatttagatt ttaccaacca
    12541 ggtccagaga cggagccaga gttgcttcac cttctttcca tttagccggg caaacttcac
    12601 ctgggtgaga agctacgtac tgagctgctt tgattttacg cagcagatca gacgcgtcac
    12661 ggccaatgcc ttcagcggta acttcgattg cctggatgat accctgcggg tcaacaacga
    12721 aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt gtcgaagtta cgggtcaggg
    12781 cgccagtcgg gtcgccgatc atcgcatatt tgattttagc gatggtttca gagctgctgt
    12841 gccatgcttt gtgggtgaag tgagtatcgg tagatactgc gtatacgtct acgcccagtt
    12901 tctgcagttc ttcgtagtgg tcagcaacgt cacccagttc ggtcgggcat acgaaagtaa
    12961 agtcagccgg gtagaagaag aagacgctcc agcggccttc ggtatctttt tcggtgattt
    13021 cgatgaattc gccgtttttg aatgcctggt ttttaaaagg tttaattttg gtgttaatca
    13081 aggacatcta tacttcctcc gtgttttcga tgagatgtaa ggtaaccaat ttttgccgat
    13141 tcggctaatg cgtttccatt atcaaatcga taagttttac cttacaacct tcgtaagaca
    13201 actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa aatggccccc tttccaggtg
    13261 accgtgtaat ctgaactacc ctttggtatc ttcagcgcct gcctgcaggc tgaggtgttg
    13321 cccttacaaa tgcaacaacg acatggatta caacaccctc ataaacaaag ggcaatcacc
    13381 tgatctaagc tcttacctat gacagtgata ggttatgcct tttactcgac ttttgcactg
    13441 actgaaaaga acaaattaat gttaaaaaag atacttttac tggctctgct tcctgcaatc
    13501 gccttcgcag aggaacttcc ttccccagta aaagcgattg aaaaacaggg cattacaatc
    13561 atcaaaacgt tcgatgcccc cggaggaatg aaaggttatc tcggaaagta tcaggatatg
    13621 ggcgtcacca tctacctgac tcccgatggt aagcacgcta tctctggtta catgtacaac
    13681 gagaaaggtg aaaacctgag taacacactt atcgaaaaag aaatttacgc accagccgga
    13741 cgcgaaatgt ggcaacggat ggaacaatcc cactggctcc tcgacggtaa aaaagatgcg
    13801 ccggtcattg tctacgtctt ctccgatccg ttctgcccat attgtaaaca gttctggcag
    13861 caggcgcgcc cgtgggtaga ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt
    13921 atcaagccag aaagcccggc gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa
    13981 acctggcaac aatatgaagc ctctggttgc aagcttaagc taaacgtgcc tgcaaacgta
    14041 agtacagagc aaatgaaagt gttaagtgac aatgagaaac tgatggacga tctgggggca
    14101 aatgtcacgc cggctatcta ttacatgagt aaggaaaata cgctgcaaca ggccgtgggg
    14161 ttgcccgatc agaaaacgct taatatcatt atgggaaata aataaaaggt aattcatgga
    14221 tggttgaact atatccctgc ttttcaacca tcctaaaaaa gcatgaattt ctcgctctac
    14281 cctaagtggc tgtgctattt tagaaaaact taatcctgag ttaagcaaaa aaacataatc
    14341 aataaaaata catacgcaaa aaacattgat taagtgaata taccatggaa gaaaaatata
    14401 aaccggagta gtgtatggcc aatctctacg acttgaaaaa gttcgactta aatcttcttg
    14461 tcatatttga gtgtatttac cagcatttaa gcattagcaa agctgctgaa tcactatata
    14521 tcaccccatc agccgtgagc cagtcgttac aacgcttacg tgcgcaattt aatgaccctt
    14581 tattcattcg ctcaggaaaa ggtatcgcgc cgacgacaac agggttgaat ctgcatcacc
    14641 atcttgagaa aaacctcaaa ggccttgaac aaacaattaa tatagtcaat aagtccgaac
    14701 taaagaaaaa ctttattatc tacggccctc agctcatttc ttgctctaat aatagtatgc
    14761 tgatccgctg cctacggcaa gatgcctctg tcgagattga gtgccatgat atcctcatgt
    14821 cagcagagaa tgctgaagaa ctgttggttc accgcaaagc cgatctggtg atcacccaaa
    14881 tgccagtgat cagccgttcc gtcatttgca tgccattgca tacaattcgc aatactctaa
    14941 tttgtagtaa taagcatccg cgaattactg acaatagcac ttatgaacaa attatggcag
    15001 aagagttcac ccaattaata gccaaatcag caggagttga tgatattcaa atggaaatag
    15061 acgagaagtt tatgaatcgc aaaatttcat ttcgtggttc ctctttgctg acaataatta
    15121 atagcattgc agttacagat ttactgggaa tagtccccta tgaactttat aactaccacc
    15181 gtgactttct taatttaaaa gagataaaac tagaacatcc gttaccttca ataaagcttt
    15241 acatttccta taataaatct tctttaaata atttggtgtt ctcccgattt attgaccgtc
    15301 taaatgagag tttttgatat ataactatag acagctacta taactttcat ctatttattc
    15361 acagcgcgaa tgaaaaataa aacactctgc tttacagtgt ttactttata attaacagtc
    15421 ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat
    15481 tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctaga cactcaacac
    15541 cctaccccgc gtttgtgtct cattttctgg cggcaaagac tctggtttaa tgctgcatct
    15601 gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta tcaactggga
    15661 ggc
//
LOCUS       DAZOZX010000090        15523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZOZX010000090 DAZOZX010000000
VERSION     DAZOZX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.090"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..73)
                     /locus_tag="ACHUPT_002791"
     CDS             complement(<1..73)
                     /locus_tag="ACHUPT_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR0820306.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHUPT_002792"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHUPT_002792"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0820307.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="ACHUPT_002793"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="ACHUPT_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0820308.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHUPT_002794"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHUPT_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0820309.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="ACHUPT_002795"
     CDS             2703..2939
                     /locus_tag="ACHUPT_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0820310.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="ACHUPT_002796"
     CDS             2942..3799
                     /locus_tag="ACHUPT_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0820311.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="ACHUPT_002797"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="ACHUPT_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHUPT_002798"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHUPT_002798"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0820312.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="ACHUPT_002799"
     CDS             complement(6414..7310)
                     /locus_tag="ACHUPT_002799"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0820313.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="ACHUPT_002800"
     CDS             complement(7359..7598)
                     /locus_tag="ACHUPT_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0820314.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="ACHUPT_002801"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="ACHUPT_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0820315.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="ACHUPT_002802"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="ACHUPT_002802"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0820316.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="ACHUPT_002803"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="ACHUPT_002803"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0820317.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="ACHUPT_002804"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="ACHUPT_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0820318.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..15223
                     /locus_tag="ACHUPT_002805"
                     /pseudo
     CDS             10765..15223
                     /locus_tag="ACHUPT_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15267..>15523)
                     /locus_tag="ACHUPT_002806"
     CDS             complement(15267..>15523)
                     /locus_tag="ACHUPT_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0820319.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3559 a         4109 c         4079 g         3776 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cac
//
LOCUS       DAZOZX010000091        15137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZOZX010000091 DAZOZX010000000
VERSION     DAZOZX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.091"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..579
                     /locus_tag="ACHUPT_002807"
     CDS             <1..579
                     /locus_tag="ACHUPT_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0820320.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHUPT_002808"
     CDS             597..1235
                     /locus_tag="ACHUPT_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0820321.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUPT_002809"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUPT_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0820322.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUPT_002810"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUPT_002810"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0820323.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUPT_002811"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUPT_002811"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0820324.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUPT_002812"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUPT_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0820325.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHUPT_002813"
     CDS             complement(5552..6613)
                     /locus_tag="ACHUPT_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0820326.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHUPT_002814"
     CDS             complement(6610..8007)
                     /locus_tag="ACHUPT_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0820327.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHUPT_002815"
     CDS             8163..9161
                     /locus_tag="ACHUPT_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0820328.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUPT_002816"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUPT_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHUPT_002817"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHUPT_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUPT_002818"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUPT_002818"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0820329.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUPT_002819"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUPT_002819"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0820330.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15137)
                     /locus_tag="ACHUPT_002820"
                     /pseudo
     CDS             complement(14232..15137)
                     /locus_tag="ACHUPT_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
BASE COUNT         3695 a         4144 c         3638 g         3660 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagc
//
LOCUS       DAZOZX010000092        15119 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZOZX010000092 DAZOZX010000000
VERSION     DAZOZX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.092"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..317)
                     /gene="chbB"
                     /locus_tag="ACHUPT_002821"
     CDS             complement(<1..317)
                     /gene="chbB"
                     /locus_tag="ACHUPT_002821"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0820331.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAA"
     gene            complement(616..954)
                     /gene="osmE"
                     /locus_tag="ACHUPT_002822"
     CDS             complement(616..954)
                     /gene="osmE"
                     /locus_tag="ACHUPT_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0820332.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1156..1983
                     /gene="nadE"
                     /locus_tag="ACHUPT_002823"
     CDS             1156..1983
                     /gene="nadE"
                     /locus_tag="ACHUPT_002823"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0820333.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            2213..3100
                     /gene="cho"
                     /locus_tag="ACHUPT_002824"
     CDS             2213..3100
                     /gene="cho"
                     /locus_tag="ACHUPT_002824"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0820334.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3060..3635)
                     /gene="ves"
                     /locus_tag="ACHUPT_002825"
     CDS             complement(3060..3635)
                     /gene="ves"
                     /locus_tag="ACHUPT_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0820335.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3838..4323)
                     /gene="spy"
                     /locus_tag="ACHUPT_002826"
     CDS             complement(3838..4323)
                     /gene="spy"
                     /locus_tag="ACHUPT_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0820336.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4653..5621)
                     /gene="astE"
                     /locus_tag="ACHUPT_002827"
     CDS             complement(4653..5621)
                     /gene="astE"
                     /locus_tag="ACHUPT_002827"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0820337.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5625..6946)
                     /gene="astB"
                     /locus_tag="ACHUPT_002828"
                     /pseudo
     CDS             complement(5625..6946)
                     /gene="astB"
                     /locus_tag="ACHUPT_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(6943..8421)
                     /gene="astD"
                     /locus_tag="ACHUPT_002829"
     CDS             complement(6943..8421)
                     /gene="astD"
                     /locus_tag="ACHUPT_002829"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR0820338.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8418..9452)
                     /gene="astA"
                     /locus_tag="ACHUPT_002830"
     CDS             complement(8418..9452)
                     /gene="astA"
                     /locus_tag="ACHUPT_002830"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0820339.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9449..10669)
                     /gene="astC"
                     /locus_tag="ACHUPT_002831"
                     /pseudo
     CDS             complement(9449..10669)
                     /gene="astC"
                     /locus_tag="ACHUPT_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            11115..11921
                     /gene="xthA"
                     /locus_tag="ACHUPT_002832"
     CDS             11115..11921
                     /gene="xthA"
                     /locus_tag="ACHUPT_002832"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0820340.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12088..12798
                     /locus_tag="ACHUPT_002833"
     CDS             12088..12798
                     /locus_tag="ACHUPT_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0820341.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12803..13480
                     /gene="ydjY"
                     /locus_tag="ACHUPT_002834"
     CDS             12803..13480
                     /gene="ydjY"
                     /locus_tag="ACHUPT_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0820342.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13495..14202
                     /locus_tag="ACHUPT_002835"
     CDS             13495..14202
                     /locus_tag="ACHUPT_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0820343.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            14202..14750
                     /locus_tag="ACHUPT_002836"
     CDS             14202..14750
                     /locus_tag="ACHUPT_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0820344.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14760..15086
                     /locus_tag="ACHUPT_002837"
                     /pseudo
     CDS             14760..15086
                     /locus_tag="ACHUPT_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         3646 a         4047 c         3770 g         3656 t
ORIGIN      
        1 tttgctgcgg cttttttaat cgctgcaacc gcagccttaa gcacgcctaa accatcgact
       61 ttgccataaa gcagcgagtc aattacttca accggtttgt tgggtaacaa acgctggatt
      121 tcgggcaaca tataagcaat ctgcggccct aataacacga catcggcatt ctgacctttt
      181 tcaccagcca gtgtttccgg aaatgcttca ataatgaccg gaacttcata tttttctgcc
      241 tgtgcgcgca tttttgatac cagtaaagag atagacatgc ccgcagaaca aaacagataa
      301 atgtgtttct tttccataaa actgccctca tcgacgatta tctgtcagcc agacactccg
      361 caagccttga cctgcttcca tgctctgggt aacttgcgaa accaacatga ctttttttgt
      421 ggctgaaagg agtatacggt atagacctga gcaatagtat ttctttgacc ttataaattg
      481 tttctccttg acctgtggtt atgaccctct tcacatttcg ggcaaataat tcgcgaagat
      541 aattaagaac tcaggccaaa aaaaaccggc gcaatggccg gtttccgttg ttactcaagc
      601 tttcagacga attgattact tcgcagcctg tggatcagtg tcgtattcag cacaggtctg
      661 ataaccggag ttgatgacat gtccggtatc atctaacgcg acaaagtagg tttctgcttt
      721 accatcacgt tgacccagga tgtaggtctg gcaagtaccg cgagcatgga tcatgctcac
      781 ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc cggctcatgc cttttttgac
      841 gtctttcacc acaggctgta caaactggtc tttggtacga tcataagctg tacaacccgc
      901 cagcatggtt aataccgccg ctgcactcag aattcctgcc atattcttgt tcatattccg
      961 tcctcttgtt tatcagcgtg ttagataagc ctggaataca ttgggcgctt tttcaagccc
     1021 gtgaacgaaa cggctccgct ttcagaggat tcctgtatga cgttttaacc accattcagc
     1081 ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt tgtcttttct
     1141 gtctggaggg gttcaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg
     1201 cagattaatg ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa
     1261 acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt
     1321 gccggaaagc tgtgccagat ggcgattaat gagctgcgcc aggaaactcg caacgaatca
     1381 ctgcaattta ttgccgtacg cctgccctat ggtgttcagg ctgacgaaca agattgccag
     1441 gatgccattg cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta
     1501 ttggctagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc
     1561 aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt
     1621 gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg gattcttcac taaatatggt
     1681 gacggcggta cggatattaa tccgctgtat cgtctcaaca aacgccaggg taaacagtta
     1741 ctgacggcat taggttgccc ggaacacctt tataagaaag ccccaactgc cgatcttgaa
     1801 gatgatcgcc cttctctgcc agatgaagtg gcactcggcg tgacctatga caatatcgac
     1861 gactatctgg aagggaaaaa cctacctgaa caggtcgcca gaacaataga gaactggtat
     1921 ctgaaaaccg aacataaacg ccgtccgcca attaacgttt tcgatgattt ctggaaaaag
     1981 taataatttg cacctattgg attgtgcgaa aaagagtaat ttgttcacgc cggatgcggc
     2041 gtgaacgcct tattctacct acaaaactat gcaaattcaa tacattgcag gagtcgaata
     2101 ggcctgacag gcgtagcacg tcagacggtg taacctttgt catcaacccg cctctttttt
     2161 aatcgcctcc cgcctgttac actggataga taaccagcat tcggagtcaa cagtggtacg
     2221 gcgtttaact tctccgcggc tcgaatttga agctgcggca atttatgaat atcccgaaca
     2281 tttacattca ttccttaatg acttacccac ccgaccaggg gtgtatctgt ttcatggcga
     2341 aagtgacacc atgccgctct atatcggcaa aagcgttaat atccgcagtc gggtgctttc
     2401 tcatttacgc accccggatg aagccgccat gctacggcaa tcccggcgaa ttagctggat
     2461 ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca aagaacaaca
     2521 gccgctgttt aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc aattaaatga
     2581 aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt tcacgagccc ccaacctgtt
     2641 tggcctgttt gccaataggc gcgcagcttt gcaagcattg cagagcatcg ctgatgaaca
     2701 aaaactttgt tatggcctgt tgggactgga accgttaagt cgcggtcgtg catgttttcg
     2761 ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg aggaacatgc
     2821 gctacgcttg cgccagtctc tggagcgttt gcgagtggtg tgttggcctt ggcaaggtgc
     2881 ggtggcactg aaagaacagc acccggaaat gactcaatat catattattc aaaactggct
     2941 gtggctgggg gcggttaatt cgctggaaga agcgacaacg ttaattcgga cacccgccgg
     3001 gtttgatcac gacggttata aaattctttg taagccgctg ctttccggta actatgaaat
     3061 tactgaactt gatccggcga atgaccagcg agccagttga tttcactgaa caacagttta
     3121 ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga accagcacac gccctggtcg
     3181 gtagtgagca gtttgtcgcc cagttgccac gccccgttaa tcacaaacac caccccgccg
     3241 cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt acagacatcc
     3301 aggcgcgtca taatattgaa atccatcgac atctgccctg ccgtcagttt cgcttttacc
     3361 acctggtccg ccgtaaaggc aaaaggctgt aacggtttta aggtatggtt aaagcggtct
     3421 gcgctttcaa ggagcatctc gccgccttcc agcaacgtca ctatcctttc catgccggga
     3481 aacaaagaaa actccccatt cgccgcgatg gatgcaatgc tggcacgcca gtaaaaatca
     3541 cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac cggcagcgtt tcgccacaga
     3601 ttcaccgaca ttttacgcat atcaaagtat tccatactcg ctcccggccc gttcttttca
     3661 ttgttatcgt tcaggtaacc gtggatggtt atcggcaact cgggtgattt acgttagtgg
     3721 tgatcaggct ttttttacaa ctgtttgaga agagaaaaga aaaccgccga tcctgtccac
     3781 cgcattactg caaggtagtg gacaagaccg gcggtcttaa gttttttggc tgaaagatta
     3841 ttcagcagtt gcaggcattt taccttttgc cgctggacgt tctgtcagac gcttctcaaa
     3901 attagcatta aattgctttt tctgttccgg cgtcaggatg ttgtaaattt tgttctgggt
     3961 ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc atttttgcga tctgcgcttc
     4021 agcttttgct ttatcgaagg tatcgctggc aatgatgtca tgcattgcgc ggcgttcttc
     4081 cagcggcgga cgtttcatct ggtcacgctg gcctttcatg atttcgtgga tctgctgttt
     4141 ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc tgatgcggac cgaacttgcc
     4201 tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg gtagtggtgt ctgcggcatg
     4261 ggccaggtta gccgcgccaa gagccagggt agaggcaaca aacagtgcag ttaatttacg
     4321 catattctat atccttcctt tcagttattt attacggctt tcttaagtag cgtgccgtgt
     4381 tgacgagatt aactttactg actttagcgt caattaatca gagcaacggt aaaacaatga
     4441 aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa agaaaatttg atggagagtg
     4501 atgagagaat attacaacac gatgattttg cagagattat gaagaactat accggatgac
     4561 tggtgataaa taaagcaaat aaccaggatt aatctgtatt aatttataag aaagcaactt
     4621 aatacccgca gaatgatttc tgcgggtaag tattagctta ttttttcgag cattaatccc
     4681 gcgcgtaatc ccaacgctac caacggatta gggaataaca catactctac atcatgggtt
     4741 acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa cggcataaaa
     4801 ttcagcgtgt cacttgccat atgcatttcg aaggacggcg agtggcgagt aatttgcgaa
     4861 accacccgat aacggagcgg cggtgttctc acgataccga cactctcacc agatagcagc
     4921 gcagcaattg cgctggcagt tactgcaaac tggcgaagat cgttttgccc aaagggcaac
     4981 gctttgccaa gttccaacgt acaggccagc gcgccaaaat gtctgacgct gaaatgggta
     5041 aacgtaccac caggctcctg atggaacacc agcgcctcca gccccgccgc acccagccac
     5101 gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta atacaccgaa ctgcggatgc
     5161 aaggagccac gaattgcggt atgtagatca aggtgccaac gcacagattc tttgccctgg
     5221 tcataaaaat cttccaggca ctgttccagt tcgcgcgccc gacaggtttc tccgctttca
     5281 gcaaatagct gccaacgacc gccaaacatt cggttcatat cgctatggca ataacgtttc
     5341 ccttgcttca gcgcaggagg attcccgagg atcaccagca accgccaacg taacgggatc
     5401 tcgccgtgag atatcgcgcc aagcaacgcg tccagcattt ccacaggtgc cgtctcatta
     5461 ccgtggatcc ccgcagaaat caccagcgcg ccttgcggtg gcgttaatgg cgtcagttcc
     5521 agcacaccat cgcccagcca gcgccagcga acgccgttga tttctcgctc ggtgataacc
     5581 ggttttttac ccgttaaggt cagagcaaga aaattatcca ttgcccctcc ctcgcgctgg
     5641 aacggataaa ccgaaccgag attcagtaat tggctcaata catccagtgc ttcccgccct
     5701 tcgcgcagca attgcgggtc ggccagatcg gcagcagtaa gacgatcgcg gtagtaacga
     5761 tccacccagt cattgagcgc attaaacaag gtatcgttca tcatcaccgc cggattcacc
     5821 gcccggcgtt cttcttctgt caataccacc cgcaaccgca ggcacgccgg gccgccgcca
     5881 ttcgccatgc tttcacgtaa gtcaaagact tttagttcgc taatcgggtt gtcagcggca
     5941 aggagttcat tgagataacc ccatactccg gcgtgttccc gacactcctg aggcagcacc
     6001 aacatcatgg aaccatcatc gcggctcaac agttggctgt taaacagata ggtagacacc
     6061 gcatccgaca cggaaacctg agttgccgga acttctatcg ccataaaacc gttcacccgc
     6121 gtgccagtaa ctgtgactgg cgagcgaacg cctgttggtg gcaaaacagc acctggcggt
     6181 tactcacggc aatcacgtca ttatgaaaaa cgctctggtc gataacgtcc gggttttgct
     6241 gggcgaaaat cacctgttgg ggattcacct gattaagcct tgccaccgcc tcgctggctt
     6301 cgcgagtctg tcgcgccgga taacgggaag gccgggtatc attgccttct tctcgcccgt
     6361 agacaaaaag tagcataccc ggttcaccgt aatgaccgcc gagacgattg tggtttgccg
     6421 ccccctcatc accgagcaac gctacctgcg gcaacgccga atggacgcta aatttctctt
     6481 cgtccttaaa aatcgctttt aacagcgatt cagtgacggg cgcttccagc gaacggtgaa
     6541 atttattgtt caggttggca acggtgagat gcactttgcc atccagcgta tcggcagatg
     6601 gcgcgatcgt tgccgcattg gctacccaca ttggcgaagc ggaactgacg ctggaaagcc
     6661 agtgcggtgc ctggcgtgca actttttcca gtacctgctc atcgctacca ctgaatccca
     6721 actgacgcag caccggaata aacggacgct cgtgcggcgg aatcacggcc tgggggaatc
     6781 ccgcatcggc aagggctttc attttcagta agccctgttt tgccgccagt cgcgggttag
     6841 acacctgaaa acggtgacgg gtagaggctt cattaccaaa cgacaggccc gcgtaatgat
     6901 gcgtcagccc taccagcccg tcgaaattaa cttcccaggc gttcatcgca ccacctcatc
     6961 ggaaaaatcc agcccggggt taagcgtggc gggcaatgtt aacgagtccg actccaggct
     7021 cgccataggc catgcgcagt aatctgcggc ataccaggcg ctggggcgat ggttaccgga
     7081 agcaccaatg ccgccgaatg gggcggtact ggcagcacca gtaagcggtt tgttccagtt
     7141 aacaatcccc gcccgcgcct ccagcaacag ttgatcgaat ttttcccgct cgggggaaac
     7201 cagaccgcaa aagagtccga agcgagtgtt gttcgccatc cgaatcgcct catcaaaagt
     7261 atcataacgc cagacgcgca aaaacggccc gaacacctct tcatccggca caccgccaac
     7321 gcccgtcatt tcaatgatcc ctggtgtcag caacgatgtc cctgcttgta ataagcgcgg
     7381 cgcaagcagg gttcgtccgc ccatcgcttc cagttgctgc caggcagtaa ccacctgctg
     7441 tgcggcctgt tcagaaatca gcccgccaat aaacggctgc ggttcgtcat cccagttgcc
     7501 cggcgttaat cgctggctga cggcaaccag actagcaaga aacgcatcgc cctgcgctcc
     7561 gcttttgagc aataaacggc gggcgcaggt gcagcgttgc ccggctgtga caaacgccga
     7621 ctgaatggtc agatggacag ccgcgtcgat atccgccacc tcatcgataa ttagcggatt
     7681 attaccgccc atctcaaggg cgagaatttt ctccggctga ccggagagct ggcgatgcag
     7741 ctggtagccg gtattggcgc taccggtaaa cagcaaaccg tcgagatcct ccagcgcact
     7801 cagcgcctga cccgtttcac gcccgccctg caccaggttc agcacgcccg gcggcaagcc
     7861 agcctgctgc cataaacgca ttaccgcttc gccactccac ggtgtcagtt cgctgggttt
     7921 aaagataatg gtgttacctg ccagcaatgc cggaacgata tgtccgttcg gcaaatgacc
     7981 agggaaatta tacggcccaa acaccgccag cacgccgtgc gggcgatgtc gcaggctcgc
     8041 cgcaccgtcc ggcatttcac tacgctgctc gccggtacga acgtgatacg ccttaattga
     8101 tatcgcgatt ttattgatca tcgccgtcac ttcggttgcc gcttcccagc gcggcttacc
     8161 cgtttctctg gcaataatcg cggttaattc ggctttattg ctttccagca atccggcaaa
     8221 gcgttcgaca acgacctgac gttcagccaa tgagagccgc gcccagcgcg gaaacgccgc
     8281 acgggctgcc cgacaagcct gttcgacctg agcggcatcg gcatcattgc cttgccataa
     8341 cacctcgccc gataccggat tacgcttcac acgcgatgcg ccctggcccg ttatccagtc
     8401 accgttaatc cataaagtca tgctgttttc tcctctgcgc acaggcgcac cagacgaacg
     8461 cgatcccctg cgtggcattt gagggcatcc agttgtgcgg cggttaaaat caaacgctcg
     8521 gttgccggat cggtacgcgc cagcaccacg cggaaatggt gataattttc attggcgacc
     8581 aggcaggccg gaaaatcgcc ctgcgcaggc tgcccttctg ccacttccac cagccgactt
     8641 ttacggatgg cgcgcacgcg gtcgatgtca cactcaagcg ttggcccgcc gtcgaagatg
     8701 tcgatatagt tgcggtaacg aaaaccttct ttctccagca ccgcgcgggc aggcgcggtt
     8761 tgcggatgta cctgaccgat gacgtcctgg gcttcctggg ataaaaagtg ggtatagatc
     8821 ggatgtttcg gcatcagttc tgcaataaat gccttttgcc cggtgccgca gagaaaatcg
     8881 gcgcggctaa aatccatcga aaagaagcgt ttaccgaggc tttgccagaa cggtgaatag
     8941 ccgtgttcgt caatcacccc gcgcatttca gcaaccactt tgtcattaaa cttgtcgcga
     9001 aaagccgcca taaacataaa gcgcgatttc gacagcaaat agccgttgcc ctctttgcgc
     9061 cagtccgggt cgagaaacag cgtgcacagc tcgctgctgc cggtgtgatc gttactaaga
     9121 aacagcgtcg gcaatgcgtt atagacattc agctcttttg aggcgtgaac caacgtgccg
     9181 acgcgatagt tgtaccaggg atcgttcagc ccaaccgcca cctcaatagc gcaaatcccc
     9241 gccacggtgc ctgtctcgct atcttccagc acgaacacat agccctgctc gcttttgggc
     9301 agttcacctt gccaggtttt gattgccctt tcgatacgcg ccgaaagcgt ggcttcattg
     9361 gcgggaagcg acgtcaggcc gccgcccgtt ttgctggcaa gctgcatcag cgccgagaca
     9421 tctgagcgct caacgggacg gatgaccatc atgatgaacc tcggctaaca aagtgttcgc
     9481 aagcagctgc aaagcgatcc agtccggtcg tcacctcttc ttcgctgaca ttgagcgcag
     9541 gcgcaaaacg caccacgttg ccacccgcaa tcagtaccat cacgcctgct ttcgccgctt
     9601 ccagagagat ctgtttcgct tgcccggcgt aatcggcatt cagtacacag ccaatcaaca
     9661 aacctaagcc gcgaacttca ctgaacaaac catagcggtg attaatagta ttaagacgct
     9721 caacaaacca gtcgtgacgc tgtttaacgc cattaagcat ctctggtgtg ttgatgagct
     9781 ccagcacttt gcctgctacc gccgaggcca gcggattacc gccataggtg gtgccatgag
     9841 tgccaacggt catcacgcgg gcgcactctt cggttgccaa caacgcaccg accgggaaac
     9901 cgccgcccag cgctttggcg gtggttaaca gatcgggcgt cacgccgtag tgcatatagg
     9961 catacagctc accagtgcgc ccgacgccgg tttgtacctc atcaaaaatc agcaaggcat
    10021 tgtggcggtc acacaattca cgcagacctt gtaaaaacgc gttgctggct ggcaccacac
    10081 cgccttcgcc ctagatgggc tcgacaatca ccgcacaggt ggcgtcgtca atcagcgcgc
    10141 tggcagagtt aatatcgtta tatgcagcat gacgaatatc cgccggcagt ggcgcaaaat
    10201 cctgtgaata ggctggctgc ccacccgcac tgacagtaaa cagcgtgcga ccatgaaacg
    10261 catttttgaa cgccacgatg ccactcttat ggctgccgta gcggtcgtga gcgaatttac
    10321 gcgccagttt tagcgccgct tcgttggctt ccgcaccgga gttacaaaag aagacgcgat
    10381 cggcaaacgt ggcgtcgatc aattttttcg ccagtcgcag taccggctcg ttggtgtaac
    10441 cgttgccggt atgccagaac ttactcgcct gttcgttcag cgcttcacgc agttccggat
    10501 gcgcatggcc cagcgcgttc accgcaatgc cacccgcgaa gtcgatatac tctttcccct
    10561 gctgatccca caagcgcgaa ccttcgccac gtaccggtat aaagggtgcc ggagcgtaaa
    10621 caggtatcat ccattcatca aagttttcac gcgtaattgg ctgagacata gcgacctcta
    10681 cagtaaataa ttcgttattt gtatgttaac aataagtaat gtttgcgctg taaatgtaga
    10741 ttgcagggtt cgtgccagcc agtgataaaa gtgcataaac ggcggaggct aactggaaat
    10801 caaggagtta taaccaaacc atatgcattt aaagtgcata taaagtgaat acgtttgcga
    10861 tgtgggtgaa taaaaagaat aaaaaacgca atgttatgca gaagtaaaat ataattctag
    10921 aattgtgatc attgacgaaa tttactggaa attactgcgc cattctgacg cagcgcgcac
    10981 caaaagcggg cattttttgc gccatcgttg acatcattaa caaccatcga tcaaatcact
    11041 taacaacagg cggtaagcaa cgcgaaattc tgctaccatc cacgcactct ttatctgaat
    11101 aaatggcagc gactatgaaa tttgtctctt ttaatatcaa cggcctgcgc gccagacctc
    11161 accagcttga agccatcgtc gaaaagcacc aaccggatgt gattggcctg caggagacaa
    11221 aagttcatga cgatatgttt ccgctcgaag aggtggcgaa gctcggctac aacgtgtttt
    11281 atcacgggca gaaaggccat tatggcgtgg cgctgctgac caaagagacg ccgattgccg
    11341 tgcgtcgcgg ctttcccggt gacgacgaag aggcgcagcg gcggattatt atggcggaaa
    11401 tcccctcact gctgggtaat gtcaccgtga tcaacggtta cttcccgcag ggtgaaagcc
    11461 gcgaccatcc gataaaattc ccggcaaaag cgcagtttta tcagaatctg caaaactacc
    11521 tggaaaccga actcaaacgt gataatccgg tactgattat gggcgatatg aatatcagcc
    11581 ctacagatct ggatatcggc attggcgaag aaaaccgtaa gcgctggctg cgtaccggta
    11641 aatgctcttt cctgccggaa gagcgcgaat ggatggacag gctgatgagc tggggggtgg
    11701 tcgatacctt ccgccatgct aatccgcaaa cagcagatcg tttctcatgg tttgattacc
    11761 gctcaaaagg ttttgacgat aaccgtggtc tgcgcatcga cctgctgctc gccagccaac
    11821 cgctggcaga atgttgcgta gaaaccggca tcgactatga aattcgcagc atggaaaaac
    11881 cgtccgatca cgcccccgtc tgggcgacct tccgccgcta atttagcagc tctcctggct
    11941 caaactgggt caggagaatt aaccttgaga aaaatcaaca aactgtcagt aatgatttgt
    12001 tgcctgccgt cctttgttat accgtcactg cgtttttagt tgtctgacca cttctctatt
    12061 atcaagtttg atataggaaa ctccacgatg aacgctgagc gtaactttct gtttgcctgt
    12121 cttatttttg cgctggtcat ttacgctatc cacgctttcg gtttattcga tctgctcacc
    12181 gatttacccc acttacagac actcattcgc cagagcggat tgttcggtta tagcctctat
    12241 attctgttat tcatcaatgc ccccctcttt ctgttaccag gaagcatact ggtgatcgct
    12301 ggaggaatag tttttggccc gctcttaggg acacaactct cattaattgc cgcaacgctg
    12361 gcctcctcgt gctcattcct gctggcgcgc tggctgggac gtgatttact gctgaaatac
    12421 gtgggccata gccatacctt ccaggccatt gaaaaaggca ttgcgcgtaa cggtattgat
    12481 tttcttattc tgacccgctt aatcccgttg tttccttaca atattcaaaa ttacgcttac
    12541 ggattcacca ccatcgcctt ctggccttat acccttattt cggcactcac gaccctgccc
    12601 ggtattgtta tttataccgt gatggcaagc gatctcgcca atgaaggcat tacgctgcgc
    12661 tttattttac aactctgtct ggcgggcctg gcgctgttta ttctcatcca gctcgcaaaa
    12721 ctctacgccc gacacaaaca tgtggatctg tctgcttcgc gccgcagccc acttactcac
    12781 ccaaaaaatg aaggatagaa cgatgtcgca acattattca gtgtcatgga aaaaaggact
    12841 ggctgcactc tgcttactgg ctgttgccgg gcttagcggc tgcgatcaaa aagagaatgc
    12901 ggcggcaaaa gtggaatacg acggactttc gaacagccaa ccgctgcgcg tcgatgccaa
    12961 taaccatacg gtaaccatgc tggtgcaaat taatggtcgt ttcctcaccg acgacactcg
    13021 tcacggtatt gtgtttaaag atggctccaa cggacataaa tcgctgttta tggcttatgc
    13081 gcccccgaaa gcattttatg aagccctgaa agaggcaggt ggtacgccgg gcgaaaacat
    13141 gacgatggat aataaagaaa cgactcatgt cacaggcagc aaactggata tttcggtcaa
    13201 ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg attgttgaca gtaatggcaa
    13261 gaaactggac atgcgctttg gcggtaattt aacggcagca gaagagaaga aaacgggttg
    13321 cctggtgtgt ctggatagct gcccggtcgg catcgtcagc aatgcaacat acacttatgg
    13381 tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat gcctcagttc tcccggcgga
    13441 taacacgctg gcaacggtta cctttaaaat caccgaataa agccaagata aaggatgatg
    13501 aagatacaat cgcgtaaaat ctggtactac cgtataaccc tcatcatcct gttgttcgct
    13561 atgctgctcg catgggcgct gcttcccggc gtccatgagt ttatcaatcg cagcgttgcg
    13621 gcgtttgccg ccgtggacca acagggtata gaacgcttta ttcagtctta cggcgcactg
    13681 gcggcagttg tctcgttctt gttgatgatt ttacaggcca ttgctgcacc gctacctgcg
    13741 tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt tctggggcgg cttgctgtcg
    13801 tggaccagtt cgatggccgg cgcggcgctg tgctttttta tcgccagagt gatgggccgc
    13861 gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca gtatggacgg ctttttcact
    13921 cgctacggca aacacaccat cctggtctgt cggttattgc cttttgtccc tttcgatcca
    13981 atcagctatg ctgccggtct gacttcaata cgttttcgct cgttttttat cgccaccggg
    14041 cttggtcaat taccggcgac tattgtttat tcctgggcag gcagcatgtt aacaggcggt
    14101 actttctggt ttgtcaccga actgtttatt ctgtttgccc tgaccgtggt gatttttatg
    14161 gcgaagaaaa tatggcttga gcgccagaag aggaatgact gatgggttta ccgccgctta
    14221 gcaaaattcc tttcatttta cgtccgcagg cgtggctgca tcgtcgccat tacggcgagg
    14281 tgctaagccc gattcgctgg tgggggcgga tcccgtttat cttttatttg gtgtcaatgt
    14341 ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc ggtagtgcga tcgcttgtca
    14401 gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt ggatatcacc agtatgaaag
    14461 tcgccgagcg taccggcagc accgataaac tgctggcagt ggctgactgg cggcaaagcc
    14521 cgctctttag cgatgaagaa cggctggcgc tggagtacgc cgaagccgca agcgtcacac
    14581 cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc gcattttgac gctcaggcgc
    14641 tcaccgaact gacggcattg atcggcctgc aaaacctgtc ggctcgtttt aattctgcca
    14701 tggacattcc cgctcagggg ctgtgccgta ttccggaaaa acgttcttaa ggagggaaga
    14761 tgcgccattg tgggtggttg ctgggattgt tatcgctgtt ttctttggca acacatgcca
    14821 gtgactggca ggaaattaaa aatgaggcca aagggcaaac cgtctggttt aacgcctggg
    14881 gcggcgatac cgcaattaac cgctatctcg actgggttag cggcgagatg aaaacccatt
    14941 acgctataaa cctgaagatt gttcgtctcg cggatgccgc agacgcggtg aagcgcattc
    15001 agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt ggatctgctc tgggtgaacg
    15061 gcgaaaactt ccgcacctta aaagagtaat gccagtcagt taagcaactg actggcatt
//
LOCUS       DAZOZX010000093        14977 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZOZX010000093 DAZOZX010000000
VERSION     DAZOZX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.093"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..103)
                     /locus_tag="ACHUPT_002838"
     CDS             complement(<1..103)
                     /locus_tag="ACHUPT_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820345.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLN"
     gene            complement(519..1460)
                     /gene="fabY"
                     /locus_tag="ACHUPT_002839"
                     /gene_synonym="yiiD"
     CDS             complement(519..1460)
                     /gene="fabY"
                     /locus_tag="ACHUPT_002839"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0820346.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="ACHUPT_002840"
     CDS             complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="ACHUPT_002840"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0820347.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1939..2811)
                     /locus_tag="ACHUPT_002841"
     CDS             complement(1939..2811)
                     /locus_tag="ACHUPT_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0820348.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="ACHUPT_002842"
     CDS             complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="ACHUPT_002842"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0820349.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3503..4288)
                     /locus_tag="ACHUPT_002843"
     CDS             complement(3503..4288)
                     /locus_tag="ACHUPT_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0820350.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="ACHUPT_002844"
     CDS             complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="ACHUPT_002844"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR0820351.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5386..6282
                     /gene="yihU"
                     /locus_tag="ACHUPT_002845"
     CDS             5386..6282
                     /gene="yihU"
                     /locus_tag="ACHUPT_002845"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0820352.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6306..7184
                     /gene="yihT"
                     /locus_tag="ACHUPT_002846"
     CDS             6306..7184
                     /gene="yihT"
                     /locus_tag="ACHUPT_002846"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR0820353.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7201..8442
                     /gene="yihS"
                     /locus_tag="ACHUPT_002847"
     CDS             7201..8442
                     /gene="yihS"
                     /locus_tag="ACHUPT_002847"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR0820354.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8580..9482
                     /locus_tag="ACHUPT_002848"
     CDS             8580..9482
                     /locus_tag="ACHUPT_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR0820355.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9681..11717
                     /gene="yihQ"
                     /locus_tag="ACHUPT_002849"
     CDS             9681..11717
                     /gene="yihQ"
                     /locus_tag="ACHUPT_002849"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR0820356.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11763..13137
                     /locus_tag="ACHUPT_002850"
                     /pseudo
     CDS             11763..13137
                     /locus_tag="ACHUPT_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13180..14583
                     /locus_tag="ACHUPT_002851"
     CDS             13180..14583
                     /locus_tag="ACHUPT_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0820357.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14651..>14977
                     /locus_tag="ACHUPT_002852"
     CDS             14651..>14977
                     /locus_tag="ACHUPT_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR0820358.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3673 a         3855 c         3736 g         3713 t
ORIGIN      
        1 gatttaatag cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa
       61 cagaataact ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac
      121 ggtttaagag caaacataca acataatgat tcataacata cagagcagta aatgaatgat
      181 actgacaatg tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa
      241 aatatttatg aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      301 ctgaactaat atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata
      361 atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa
      421 aaatttataa agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg
      481 gaatgaatga gagaggcagc accgcccctc tccataagct actcttcttc gttcccgccc
      541 tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca
      601 cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc
      661 gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg
      721 ctaatcggtt tgctgtagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg
      781 cgttcacgca gcatcagcca gataagcccc caaccggtga gcgtcgccag cgagaataaa
      841 ctcccggcaa acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata
      901 aatttttgcc cggtatattg ttgaatacgc acgcccattt tttcactaag cgggatatgt
      961 tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga
     1021 gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt
     1081 tctccctgat taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta
     1141 caggtcacgc gcttaacgcc ttcctgacgc gccaccgact ccagggtcat cgccatcagc
     1201 gttcctaacc ctttgtcctg cacgtccggg tgaacgacca taaagcgaat ggacgcttca
     1261 ttgtcggcat taatatacaa tcggcctacc gccaccagat taccctgctc gtcgacgacc
     1321 atctggtgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg
     1381 ggcttacgca acatttccca gcgaaagtga tagtaacgct ctaactcttc ttctgtttgt
     1441 ggaacctgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc
     1501 tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat
     1561 cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat
     1621 agtcatataa cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc
     1681 cccgttcggt atctgcggcg agggtaaact gggaaaccac cagtacactg ccgcccgcct
     1741 gttgcacgtt gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagcacac
     1801 gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca
     1861 aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta
     1921 cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag
     1981 tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt
     2041 ccagtagacc caaacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata
     2101 tgacgggaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc
     2161 ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct
     2221 gtacagcaac cagaaggaga tccacgacaa cagcaacgga aaaatacgca gcacgttgtc
     2281 gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag
     2341 actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg cgaacgagta
     2401 aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta
     2461 catcaacaat aacgcggtga cgatcagccc gcacgcccca acggcggtca tcttgttgga
     2521 attagcaaca aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt
     2581 ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa aagcggcgaa
     2641 aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt
     2701 acctgccagg gttgtcatgt tgtcctcatc aatacgttgc cagagtagtt ttagccaggc
     2761 ccatagtgga cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc
     2821 gcgaaatagt ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga
     2881 ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa
     2941 ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct
     3001 tgcgacagat agatatggtc agcagcatca cgaatttccg ggtattcttc cggccagaag
     3061 gtggtatgca ggcggttggt attggaaagc accaccacgc gatgcccctg ctcacgcagt
     3121 ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga
     3181 gagaactgct cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct
     3241 tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct cccccatatg aaaactcttc
     3301 ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg ctcccagcac acggttaaag
     3361 tcgatatcga caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc
     3421 gccacaagaa attaactgta gcgggaaagg atgggtttgg ctatgtggca aggtatgaag
     3481 ccaggttaac ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt tcaatttggc
     3541 tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga
     3601 cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga
     3661 ccagcagaat atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgtttacat
     3721 caaactccat caacgcgcca tcgctctcaa tcgccccaac gcttgttacc agataatcag
     3781 cacgaaaatc agccacaaag gacgccgctg aagggccaat gatcccgcta ttatgagagc
     3841 gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca
     3901 cacgcaggct gttggtgatt atccgcaaat gattatggtt aagtaacgcc cgggcaacat
     3961 gctcaacagt cgtaccaatg gtgataaata ttgttgaacc atcaggaata tagtctgcca
     4021 ccgcttcggc aatcgctttt tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg
     4081 tattaacgac gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct
     4141 cgcttaattt acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca
     4201 gctcatcaat attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg
     4261 ggttaccggt tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat
     4321 tttatacaaa aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc
     4381 caccgggacg tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta
     4441 aatccccact tgttgccagc gccaccgcca aagcgccgtg aaaaacatct cccgcacctg
     4501 tggtatctac cacatcaact ttgaaggccg gttgatgctg acgcccacca ttttccagcc
     4561 agtcgcagcc tgcgctaccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct
     4621 gttttagcgc actggccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa
     4681 aggccgcgtg atcgcttaat gccaccagct cactgatatc ctgcggcgta atgtccccgt
     4741 ccagaacggt catcacaccc gcctgacggg ccagggtgaa ggctttttta gcgccgtcgt
     4801 gccagcgtac atctgccagc acaacatccc actgagagaa atcaatttcc tccaaccact
     4861 ctgcgtcagg cagcagatcc gggctggggt agttaatgat tatccgctcg cctttggtat
     4921 ccaccatgat ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt
     4981 taacgcccca ggattccagc tctgccagca ggctgttgcc cgcgtcgtca tcacctacgc
     5041 gaccaataaa atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg
     5101 gcccgccacc aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaacc
     5161 cttccacgta atagatgcga tccatcacgg ttatacctac acaagcaaca cgaatcatgg
     5221 tcattcctta aacattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga
     5281 cggctgtcaa ttttttgacg ataaattaca aattcgatca aaaacagaca agcaaagcat
     5341 gtcataaatg acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg
     5401 tttatcggtt taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat
     5461 caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg
     5521 actcccgccg ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg
     5581 ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta
     5641 tctaccgatg cgctggtcat tgatatgtcc accatccatc cgctgcaaac cgataaattg
     5701 attgccgata tgcaagccaa aggcttcagc atgatggatg ttccggtagg ccgtacttct
     5761 gcaaatgcca ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa
     5821 cgtgccacac cgatcctgat ggcgatgggc agtgagttga tcaacgctgg cggtccgggc
     5881 atggggatcc gcgttaagct catcaacaac tacatgagca tcgcgctcaa tgcgctttcg
     5941 gcagaagccg ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg
     6001 atgagcggta ccgccgccgg taaaggtcac ttcaccactt cctggccaaa caaagtcctc
     6061 agcggagatc tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc
     6121 cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg gggccgcctc acgggaggtt
     6181 tatagccagg cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggaacag
     6241 gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata
     6301 aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc atcgggcggt tttgccatgc
     6361 tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg gcacccgctc
     6421 cggtagccga tagcgtttta actgatttca aagttaacgc tgcaaaggcc ctctcgcctt
     6481 atgcctcggc gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg
     6541 cgattgccaa aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta
     6601 ttccggtcga tagcgtggtt attgaccgca aaatcaatcc gctacagatc aaacaagacg
     6661 gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgtc
     6721 tggatatggt gaaagagttc aacgaactgt gccactcaca cggtctggta agcatcattg
     6781 agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcgatcatcg
     6841 atgccgccaa agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt
     6901 atggcaaagg tccgcaacaa gagcttctct gtgcttcaca acgtctgaat gaccatatca
     6961 atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa actgttcccg cgtgccgtac
     7021 gcgtggcaat gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc tgggcatcgg
     7081 tcgtcggttt accagacaac gagctgatgc tccgtgacgt ttgcgcaccg aaattacaac
     7141 aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag gataaaagga
     7201 atgaaatggt ttaacaccct aagccacaac cgttggctgg aacaggaaac cgaccgcatc
     7261 tttgattttg gtaaaaattc cgtagtgccg actggttttg gctggttagg caataaaggg
     7321 caaatcaaag aagagatggg cacccatctg tggatcacag ctcgtatgtt gcacgtttat
     7381 tccgttgctg cggcgatggg tcgacctggc gcttacgcgt tggttgatca cggtattaaa
     7441 gccatgaacg gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg cgtgaatgac
     7501 gagggcgtgg tggatgcctc caaacagggc tatcaacact tctttgctct gctgggtgcc
     7561 gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta caccattgag
     7621 attatcgaga aatatttctg gagcgaagaa gagcagatgt gcctggagtc ctgggacgaa
     7681 gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc ggtggaagct
     7741 ttcttgattg tttatgacgt cactcacgac aaaaaatggc tggatcgcgc gattcgcgtg
     7801 gcttccgtga ttatccacga cgtcgccaga aataatcatt atcgcgttaa cgaacacttc
     7861 gatacccagt ggaatccgct gccggattac aacaaagata acccggcgca tcgcttccgc
     7921 gcgttcggag gtacaccagg ccactggatc gaatggggcc gtttaatgct gcacatccat
     7981 gctgccctgg aagcccgttg cgaacaacca ccagcatggc tgctagaaga tgccaaaggt
     8041 ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg gagcggacgg gattgtttat
     8101 accgttgact gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc tatcgtcgaa
     8161 gcaatgggta cggtctacgc gctctacacc gtcactggcg atcgccagta tgaaacctgg
     8221 tatcaaacat ggtgggagta ctgcattaag tacctgatgg actatgaaaa tggttcctgg
     8281 tggcaggagc tggatgcgga caataaggtc atcaccaaag tctgggacgg caaacaggat
     8341 atttatcacc tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc cggcatggct
     8401 ccagcggttg cagcgggtct gctggatatt aatgcgaaat aactgatctg aaaatagggt
     8461 gcgggttttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccggtgcag
     8521 tttttgggtt tgacatcagc ctcacgggag gctgtatgtc gttaattaag gtgccttgta
     8581 tgcaaatcac caatatgcat tgcagtggac agaccgtttc acttgccgcg ggggattatc
     8641 acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag ggttgtcatt
     8701 tagtcatccc gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa gtgcttattc
     8761 cgtggccgaa ccggatcgcc aacggctgtt accgttatca ggggcaggaa tatcagttac
     8821 ccattaatga acatagttca aaagccgcta ttcacgggct gcttgcctgg cgagactggc
     8881 aaattagtga attaaccacc accagcgtta ccctgacagc ttttctgccg cccagttacg
     8941 gctatccgtt tatgctggct tcacaagttg tttattcgct caacgcacac acgggtcttt
     9001 ccgttgagat cgcaagccag aatatcggca ctgtcgcagc gccttatggc gtgggtattc
     9061 atccttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag cttccggcaa
     9121 accaggttta tgccgtcgat gaacacgtaa atccgacaac gcttcaccac gttgatgagc
     9181 tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac cacaccttta
     9241 aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc acagcaggcg ctttcggtaa
     9301 gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg tgaaaaatta cagcgtcagg
     9361 gactggcagt tgaacctatg agttgcccgc ctaacgcctt taattcaggt atcgacttat
     9421 tattactgga accaggaaaa ccgcaccgcc tgttttttaa tatttacgga cagagaaaat
     9481 aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta aaggcaacat
     9541 tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt tttatatatc
     9601 agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca atctcgttct
     9661 caactctatg gagattagtt atggatacgc cacgtccaca gttattagat tttcaatttc
     9721 atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta acccataaca
     9781 aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg ttccgcggta
     9841 atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc atcgtcagcc
     9901 agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc gccacgctga
     9961 atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac aaccttaacc
    10021 acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac ggctgcggcg
    10081 aacagttttc ctacttcgat ctgcgcggca aaccgttccc gctatggacc agtgaacaag
    10141 gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg
    10201 gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg cagaagtatt
    10261 actgccatgt tgataacagt tgctacatga acttcgactt tagcgccccg gaataccatg
    10321 aactggcgct gtgggaagac aaagctacgc tacgttttga atgtgctgac acatacatct
    10381 ccctgctgga aaaattaact gccctgctgg gacgccagcc agaactgccc gactggattt
    10441 atgacggggt aacgctcggc attcagggcg ggacggaagt gtgccagaag aaactggaca
    10501 ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg tctgggatcc
    10561 gtataacctc ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc
    10621 cgcaactgga ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc ctggcctata
    10681 tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa cacggctatc
    10741 tagcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt tacggcggcg
    10801 ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc aaaaagaaca
    10861 tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg cccaccgaca
    10921 cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct gcgctgtggg
    10981 cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc ctcttcttta
    11041 tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg ggcgaccaga
    11101 acgtggactg gagtctcgac gatggcctgg cgtccgtagt cccggcggcg ctgtcgctgg
    11161 caatgaccgg acatggtctg catcacagcg acattggcgg ttacaccacc ctgtttgaga
    11221 tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc acgccgatga
    11281 tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc gatgcggaaa
    11341 ctatcgccca ttttgcccgt atgactaccg ttttcaccac cctgaaacct tatctgaaag
    11401 aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg ttcctgcatt
    11461 acgaagacga tgcacatacc tattccctga aatatcagta cctgttaggc cgcgacattc
    11521 tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac gctctatctg ccggaggata
    11581 actgggtaca tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc gttaatgcgc
    11641 ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca ctgttcgcgt
    11701 cgttaaacag catctaatca catccgcccg tggggaaact catgggcaaa gggagaacat
    11761 cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt aaagagaaac
    11821 tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt acgctttatc
    11881 ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc attatctttt
    11941 tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg ttggattccc
    12001 gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg tcattcccgg
    12061 tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc ggtaaaacgg
    12121 tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg aactgctcct
    12181 acggcgcaat ggtcccggca atcaccaaaa accccaacga acgcgcctca ctggcggcat
    12241 ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc gtgccagtta
    12301 tgaatcttat cgaaggtaat cagaaacttg gctatatctt cgccgccacg ctgttttcac
    12361 tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa
    12421 cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc gcaattgccg
    12481 gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg gcgtttaacg
    12541 tcaagctcgc cattcaggtc tattacaccc agtacgtact taacgatccc atcctgttgt
    12601 cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg atgcctggcg
    12661 cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt tgggtgctgg
    12721 gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc
    12781 tggcgttctt cggctcagcg cctgaactgg gcgctggttt ccgacaccgt cgagtacggc
    12841 gagtggcgca ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac cttctttcgc
    12901 aaagtgtctc aggcgctggc tggtttcttc cccggctgga tgctgacgca aattggctat
    12961 gtgccgaacg tcgcacaggc tgaccacacc attgaagggt tacgccagtt gatcttcatc
    13021 tacccaagcg cactggcggt agtcaccatt gtggcgatgg gttgcttcta cagcctgaac
    13081 gagaagatgt atgtccgcat tgtggaagag atagaagccc gtaaacgcac ggcgtaataa
    13141 taataaacga cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg
    13201 acattaaaac tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat
    13261 ttaatgctct gcatcggtac tctgtatctc ctcaaacttt ataccgatga attagggatg
    13321 cctgcttact atggcgggat tatctttctg gtcgcgaagt tttttaccgc gtttaccgat
    13381 atgctcaccg gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt
    13441 ccctttattt tatatgcagc agttccggcg gcgttaattg caacgctcca gtttatcgcc
    13501 accacctttt gtttgccggt taaaacgacg attgccacca cgctgtttat gatgtttgga
    13561 ctttcataca gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac
    13621 ccgaatgagc gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg
    13681 atttgtaccg tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat
    13741 gcctgtgcgg cacttatgtt ctccattggc ggctttattt ttatgatgct gtgctacaga
    13801 ggcgtcaaag agcattatgt ggacacagcg ccaaccggac ataaagccag tattctcaaa
    13861 tctttttgcg cgatatttcg taatccacca ttgctggttt tatgcattgc taacctgtgt
    13921 actctggcgg catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg
    13981 aatgatatta atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc
    14041 gtattactgg tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc
    14101 atggtgctat gggcggtggg tgatatactg aattatttct ggggaagtaa ctctttcact
    14161 ttcgtcatgt tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg
    14221 gcactggtac cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt
    14281 tctgtttata ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg
    14341 ccaggcatta tgctgacgca aattggttat gttcccaaca tcgcgcaaag cgatgcgaca
    14401 ttacagggtt tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca
    14461 ttgacgatgg gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa
    14521 atcaaccaac gcaaaaataa agagatggcg acagaaaaaa aaacggcttc cgtaacgtta
    14581 taaatgtcaa tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa
    14641 tggacttatt atgaaaaaga ttaatgcaat aattttatta tcttctctga cctctgcctc
    14701 ggtatttgct ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg
    14761 cgaggttatg ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa
    14821 tacctacacc tttcagcgag aagatgaact aaaacatggc tataatgaaa ttgaaggttg
    14881 gtatccttta ttcaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga
    14941 taagagtatc agttcgggtg gtgcagtgta tctggac
//
LOCUS       DAZOZX010000094        14703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZOZX010000094 DAZOZX010000000
VERSION     DAZOZX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.094"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(7..1269)
                     /locus_tag="ACHUPT_002853"
     CDS             complement(7..1269)
                     /locus_tag="ACHUPT_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0820359.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(1467..1542)
                     /locus_tag="ACHUPT_002854"
     tRNA            complement(1467..1542)
                     /locus_tag="ACHUPT_002854"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1507..1509),aa:Phe,seq:gaa)
     gene            complement(1648..2355)
                     /locus_tag="ACHUPT_002855"
     CDS             complement(1648..2355)
                     /locus_tag="ACHUPT_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0820360.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2508..2591
                     /gene="yqgH"
                     /locus_tag="ACHUPT_002856"
     CDS             2508..2591
                     /gene="yqgH"
                     /locus_tag="ACHUPT_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0820361.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2753..4888
                     /gene="speC"
                     /locus_tag="ACHUPT_002857"
     CDS             2753..4888
                     /gene="speC"
                     /locus_tag="ACHUPT_002857"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0820362.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4937..6193)
                     /gene="nupG"
                     /locus_tag="ACHUPT_002858"
     CDS             complement(4937..6193)
                     /gene="nupG"
                     /locus_tag="ACHUPT_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0820363.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6395..7474)
                     /gene="mltC"
                     /locus_tag="ACHUPT_002859"
     CDS             complement(6395..7474)
                     /gene="mltC"
                     /locus_tag="ACHUPT_002859"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0820364.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7539..7814)
                     /locus_tag="ACHUPT_002860"
     CDS             complement(7539..7814)
                     /locus_tag="ACHUPT_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0820365.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7842..8894)
                     /gene="mutY"
                     /locus_tag="ACHUPT_002861"
     CDS             complement(7842..8894)
                     /gene="mutY"
                     /locus_tag="ACHUPT_002861"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0820366.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9055..9774
                     /gene="trmB"
                     /locus_tag="ACHUPT_002862"
     CDS             9055..9774
                     /gene="trmB"
                     /locus_tag="ACHUPT_002862"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0820367.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9774..10100
                     /locus_tag="ACHUPT_002863"
     CDS             9774..10100
                     /locus_tag="ACHUPT_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR0820368.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10284..11003
                     /locus_tag="ACHUPT_002864"
     CDS             10284..11003
                     /locus_tag="ACHUPT_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0820369.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11179..12225
                     /gene="ansB"
                     /locus_tag="ACHUPT_002865"
     CDS             11179..12225
                     /gene="ansB"
                     /locus_tag="ACHUPT_002865"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0820370.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12342..13304
                     /locus_tag="ACHUPT_002866"
     CDS             12342..13304
                     /locus_tag="ACHUPT_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0820371.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13405..>14703)
                     /locus_tag="ACHUPT_002867"
     CDS             complement(13405..>14703)
                     /locus_tag="ACHUPT_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR0820372.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3835 a         3549 c         3790 g         3529 t
ORIGIN      
        1 tgcttactat tttaatggat tgttaatctt tgcatattca aacgggctga tacccttctc
       61 ccggttcgca tctagaaaat cggcccacca ttgcaacatc agtcgccgtt cttccagatg
      121 ttctgcttta tggatatacg cagcacgtac accattacgc tcctgatggc tcatctgacg
      181 ttccacagca tcgcgggacc acaaacctga ttcaatcaat gcactgcacg ccatcgcccg
      241 gaatccatgg ccacagactt cctgggttgt gtcatacccc atcaccctca gggctttatt
      301 tacagtattt tcactgattg gtttacgcgg gtcatgtgca cccgtaaaga tcagaccatt
      361 ttcaccagcc catgtctgaa gttctgccag tatggctaca acctgtttgc tgagtggcac
      421 aagatgtggt gttcgcatct tggatccccg gtgtgagaat tttacgcctg gcagaggttc
      481 gcgttcggct ggaatagtcc acatggaatt gtcaatatca atttccgacc agcgggcata
      541 tctgagttca ctggaacgaa taaaaaccag taaattcagc ataactgcca gtcgggtgac
      601 aggctggcct ttataaccgt caatacgcga cagaagttca ggcaggcgtg aaagctccag
      661 agcagggcga tgctggcgtt ttaccgtggt caatgcacca gccatatcca gagccgggtt
      721 atatctgatg atgccagact gtacggcgta acgcattaca gcagatattc tttgctgcag
      781 acgactggcg atttcataaa tttgttttgc ttctgcagca cgaacaggga taagcaggtc
      841 cggggtattg agtgttgtga tatcccggtt gccgataaag gggaaaacgt gtgtctcgag
      901 gcttttgagt actttatcgc tgtgtgattg tgcccattgt ttattactgg catgccagcg
      961 tcgtgcaata tgttcaaaag agagctgtaa atcagggaca acttttttaa ctcttttctg
     1021 ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc tcatcccgtt ttgtacgagc
     1081 ttcggagaga gaaacttctg gatacacacc aagtgccaga gtcttctcct tacccagaat
     1141 acgataacgg agacgccagt aacgggaacc attaggatga accagcagga acatgccggc
     1201 cccgtcagtg agtttataag ccttgtcagt gggctttgca gcccggattt ttgcgtcagt
     1261 cagtgccata gttcagcctt ctgaatgcgt taaagagggt acaaattctg tcgaactggg
     1321 gtatacccgc aattataccc acacaatggg gttgatgtgg ggggaatctg gttgagttcg
     1381 gtagaattga tttggaggaa gaacacttaa atcatggtgt tctgaaacga aaaacggacc
     1441 tccgtggagg tccgttgata tgaatttggt gcccggactc ggaatcgaac caaggacacg
     1501 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1561 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1621 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1681 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1741 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1801 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1861 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1921 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1981 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     2041 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     2101 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     2161 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     2221 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2281 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2341 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2401 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2461 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2521 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2581 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2641 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2701 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2761 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2821 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2881 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2941 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     3001 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     3061 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     3121 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     3181 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3241 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3301 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3361 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3421 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3481 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3541 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3601 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3661 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatct cgatgatggc
     3721 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3781 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3841 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3901 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3961 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     4021 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     4081 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     4141 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     4201 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4261 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4321 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4381 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4441 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4501 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4561 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4621 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4681 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4741 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4801 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4861 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4921 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4981 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     5041 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     5101 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     5161 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     5221 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5281 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5341 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5401 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5461 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5521 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5581 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5641 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5701 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5761 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5821 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5881 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5941 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     6001 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     6061 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     6121 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     6181 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6241 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6301 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6361 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6421 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6481 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6541 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6601 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6661 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6721 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6781 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6841 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6901 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6961 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     7021 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     7081 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     7141 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     7201 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7261 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7321 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7381 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7441 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7501 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7561 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7621 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7681 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7741 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7801 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7861 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7921 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7981 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     8041 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     8101 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     8161 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     8221 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8281 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8341 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8401 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8461 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8521 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8581 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8641 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8701 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8761 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8821 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8881 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8941 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     9001 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     9061 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     9121 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     9181 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9241 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9301 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9361 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9421 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9481 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9541 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9601 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9661 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9721 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9781 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9841 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9901 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9961 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
    10021 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
    10081 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
    10141 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
    10201 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10261 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10321 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10381 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10441 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10501 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10561 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10621 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10681 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10741 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10801 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10861 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10921 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10981 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    11041 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    11101 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    11161 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    11221 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11281 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11341 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11401 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11461 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11521 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11581 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11641 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11701 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11761 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11821 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11881 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11941 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    12001 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    12061 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    12121 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    12181 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12241 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12301 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12361 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12421 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12481 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12541 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12601 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12661 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12721 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12781 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12841 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12901 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12961 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    13021 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    13081 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    13141 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    13201 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13261 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctagaaaaaa gataccgaac
    13321 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13381 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13441 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatagcag
    13501 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13561 actacccacc ggcggtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13621 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13681 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13741 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13801 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13861 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13921 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13981 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    14041 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc
    14101 acggcgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    14161 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    14221 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14281 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14341 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14401 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa
    14461 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14521 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14581 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14641 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14701 atc
//
LOCUS       DAZOZX010000095        14658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZOZX010000095 DAZOZX010000000
VERSION     DAZOZX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.095"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHUPT_002868"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHUPT_002868"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0820373.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHUPT_002869"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHUPT_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0820374.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHUPT_002870"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHUPT_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0820375.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHUPT_002871"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHUPT_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0820376.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACHUPT_002872"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACHUPT_002872"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0820377.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="ACHUPT_002873"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="ACHUPT_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0820378.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="ACHUPT_002874"
     CDS             5920..6417
                     /locus_tag="ACHUPT_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0820379.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="ACHUPT_002875"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="ACHUPT_002875"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0820380.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHUPT_002876"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHUPT_002876"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0820381.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="ACHUPT_002877"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="ACHUPT_002877"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0820382.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="ACHUPT_002878"
     CDS             9102..9665
                     /locus_tag="ACHUPT_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0820383.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHUPT_002879"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHUPT_002879"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0820384.1"
                     /translation="MSGILLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="ACHUPT_002880"
     CDS             complement(10257..11237)
                     /locus_tag="ACHUPT_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0820385.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="ACHUPT_002881"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="ACHUPT_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0820386.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="ACHUPT_002882"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="ACHUPT_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0820387.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /gene="yggU"
                     /locus_tag="ACHUPT_002883"
     CDS             12540..12830
                     /gene="yggU"
                     /locus_tag="ACHUPT_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0820388.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="ACHUPT_002884"
     CDS             12838..13431
                     /locus_tag="ACHUPT_002884"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0820389.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="ACHUPT_002885"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="ACHUPT_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0820390.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3539 a         3875 c         3759 g         3485 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaatcttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgaca
//
LOCUS       DAZOZX010000096        14481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZOZX010000096 DAZOZX010000000
VERSION     DAZOZX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.096"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHUPT_002886"
     CDS             <1..118
                     /locus_tag="ACHUPT_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820391.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACHUPT_002887"
     CDS             complement(115..381)
                     /locus_tag="ACHUPT_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0820392.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUPT_002888"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUPT_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0820393.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="ACHUPT_002889"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="ACHUPT_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="ACHUPT_002890"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="ACHUPT_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0820394.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHUPT_002891"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHUPT_002891"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0820395.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHUPT_002892"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHUPT_002892"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0820396.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHUPT_002893"
     CDS             complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHUPT_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0820397.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHUPT_002894"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHUPT_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0820398.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHUPT_002895"
     CDS             complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHUPT_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0820399.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHUPT_002896"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHUPT_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHUPT_002897"
                     /pseudo
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHUPT_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12643..14097)
                     /locus_tag="ACHUPT_002898"
     CDS             complement(12643..14097)
                     /locus_tag="ACHUPT_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR0820400.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14481)
                     /locus_tag="ACHUPT_002899"
     CDS             complement(14094..>14481)
                     /locus_tag="ACHUPT_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0820401.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3651 c         3418 g         3551 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tacaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc a
//
LOCUS       DAZOZX010000097        14477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZOZX010000097 DAZOZX010000000
VERSION     DAZOZX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.097"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHUPT_002900"
     CDS             <1..185
                     /locus_tag="ACHUPT_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0820402.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="ACHUPT_002901"
     CDS             complement(210..401)
                     /locus_tag="ACHUPT_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0820403.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="ACHUPT_002902"
     CDS             complement(506..742)
                     /locus_tag="ACHUPT_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR0820404.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHUPT_002903"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHUPT_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHUPT_002904"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHUPT_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHUPT_002905"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHUPT_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0820405.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="ACHUPT_002906"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="ACHUPT_002906"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0820406.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="ACHUPT_002907"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="ACHUPT_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0820407.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="ACHUPT_002908"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="ACHUPT_002908"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0820408.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="ACHUPT_002909"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="ACHUPT_002909"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0820409.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMMMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="ACHUPT_002910"
     CDS             complement(10860..12233)
                     /locus_tag="ACHUPT_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0820410.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHUPT_002911"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHUPT_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0820411.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="ACHUPT_002912"
     CDS             complement(13344..13583)
                     /locus_tag="ACHUPT_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0820412.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHUPT_002913"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHUPT_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0820413.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="ACHUPT_002914"
     CDS             13960..14247
                     /locus_tag="ACHUPT_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0820414.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3805 a         3524 c         3707 g         3441 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atgatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat ttattaggat gtttacatca gatttgtgat taagcgtggt
    14461 attatttatt agcgaaa
//
LOCUS       DAZOZX010000098        14318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZOZX010000098 DAZOZX010000000
VERSION     DAZOZX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.098"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..3052
                     /locus_tag="ACHUPT_002915"
                     /pseudo
     CDS             1..3052
                     /locus_tag="ACHUPT_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3062..6708
                     /locus_tag="ACHUPT_002916"
                     /pseudo
     CDS             3062..6708
                     /locus_tag="ACHUPT_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6769..7176
                     /locus_tag="ACHUPT_002917"
     CDS             6769..7176
                     /locus_tag="ACHUPT_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820415.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7718..8806
                     /locus_tag="ACHUPT_002918"
     CDS             7718..8806
                     /locus_tag="ACHUPT_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR0820416.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8817..11096
                     /locus_tag="ACHUPT_002919"
     CDS             8817..11096
                     /locus_tag="ACHUPT_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR0820417.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11089..12225
                     /locus_tag="ACHUPT_002920"
     CDS             11089..12225
                     /locus_tag="ACHUPT_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0820418.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12222..14225
                     /locus_tag="ACHUPT_002921"
     CDS             12222..14225
                     /locus_tag="ACHUPT_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820419.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3755 c         3654 g         3363 t
ORIGIN      
        1 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
       61 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
      121 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
      181 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
      241 catctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
      301 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
      361 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
      421 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
      481 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga
      541 atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta
      601 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
      661 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      721 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      781 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      841 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      901 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      961 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     1021 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     1081 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     1141 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
     1201 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
     1261 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     1321 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
     1381 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     1441 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     1501 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
     1561 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1621 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1681 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1741 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1801 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1861 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1921 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1981 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     2041 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     2101 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     2161 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     2221 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     2281 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     2341 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     2401 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     2461 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     2521 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     2581 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2641 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2701 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2761 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2821 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2881 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2941 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     3001 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     3061 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     3121 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     3181 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     3241 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     3301 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     3361 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     3421 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     3481 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     3541 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3601 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3661 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3721 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3781 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3841 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3901 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3961 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     4021 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     4081 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     4141 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     4201 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     4261 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     4321 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     4381 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     4441 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     4501 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     4561 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4621 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4681 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4741 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4801 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4861 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4921 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4981 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     5041 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     5101 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     5161 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     5221 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     5281 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     5341 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     5401 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     5461 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     5521 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     5581 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5641 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5701 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5761 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5821 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5881 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5941 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     6001 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     6061 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     6121 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     6181 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     6241 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     6301 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     6361 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     6421 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     6481 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     6541 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6601 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6661 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6721 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6781 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6841 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6901 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6961 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     7021 cctgaacctg taatagtaat ttttaattgt aaactacaga atatattcaa ccaacaaata
     7081 gaattatata ctctagataa ttcaagtcgc agcacatgca acttgaagta tgacgagtat
     7141 agctttatta aaggggggaa cggtattccc ccttagatgc gtttcaattt gcccgttact
     7201 tcaacaaatc aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg
     7261 ttaacttttg tgtcagcgtg tccatccgct ctccttatta aggagcacaa caccagcacc
     7321 ttacccttac actgcctgtc gatactggtt tacgtttgtt gagtcgccat gttgcactcc
     7381 ggatcagagc actacctgcg gcaccacccg tccggtagcc aggacttgaa ataggttcga
     7441 tccggcgcaa cttcccaaca gtcaggatta taaatctcat tatcttaatt gcaacactgt
     7501 ccagaggtgg ttataattcg ttcgttggaa cacgatgttg cacaggcagt ggtgttaggc
     7561 ctgaaaatag taaataaaag aacctgcctc accagcagtt ttttttattt actgtgatct
     7621 gctttccaga tatttttcgc tcaaacaact aatgcgccaa acatttattg cgcgtaaaat
     7681 atcgtttatt tcattaatac atttcaggga tgaatatatg tcaccgcaga ataatcatct
     7741 gcagcgtccg cctgctgctg tgttatacgc cgatgaactg gcaaaattaa aacaaaatga
     7801 taacgcacct tgcccgcccg gttggcagtt aagtttgcct gcggcccgtg cttttatcct
     7861 tggcgatagc gcgcaaaata ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg
     7921 tatgttagtc actcttgcca ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc
     7981 aaaatctctc ctgtctgaat tactggcaac ctctattagc ggcgatgccg ggttaaccat
     8041 ccagggcggc gcatctacta ccgaagatca aatcaagtac ggctggaact acgccctgct
     8101 tatcaatcat ggtccatcaa cggaagcgct ggtccctgcc ccgctttacc agggaatgcg
     8161 cgacggcaaa atcgtccgtt ttgaagagat aacgcgtaca ccgctggaag tgcaagactg
     8221 tctgttaggt atgctttctg acagggtgat gacggtgccg gaactcactg gtgaagccag
     8281 tcagctctat gcgcgagaag gctttaatat tattgccact gccaataccc gcgatcgcgg
     8341 cgtcaatgaa atgagtgctg cgctaaagcg tcgcttcgat tttgaaactg tctttccgat
     8401 tatggatttc gcccaggagc tggagcttgt cgccagtgct tcggcgcgtt tgctggccca
     8461 tagcggtatt ccgcataaag tgcctgacgc ggtactggag ttactggtcc gcaccttccg
     8521 cgatctgcgt gccaacggcg aaaagaaaac gtcaatggat acactaacag cgattatgtc
     8581 caccgctgaa gccgtgaacg tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa
     8641 tcgcgcaggt gagccagccg acctggtgga ctgcattgcc ggaaccatcg tcaaagataa
     8701 cgaagaagat cgcgctcgtc tgcgccgtta cttcgaacag cgtgtcgcca cacataaaga
     8761 agctcactgg caggcttatt atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga
     8821 gcgagccgtt aattgtcggc atccggcatc atagtccggc ctgcgcccgg ctggtgaaat
     8881 cgttaatcga aagccagcgg ccacgatacg tgttgattga aggccctgct gattttaatg
     8941 accgggtaga cgaactgttt ttagcccacc agcttccggt agctatttac agttattgcc
     9001 agtatcagga cggtgcagcc cccgggcgtg gtgcctggac gccatttgct gaattttcgc
     9061 cggagtggca ggcgctacaa gccgatcgtc gtattcaggc acaaacttac ttcatcgatt
     9121 tgccttgctg ggcgcagagt gaagaagagg acgattcgcc tgatacgcaa gaggaaagcc
     9181 agaccttgct gctgcgtgcc acccgcatgg ataacagcga caccctgtgg gatcacttgt
     9241 tcgaagatga aagccagcaa actgcattac cctctgcact ggcgcactat tttgcccaac
     9301 tgcggggcga ctcccccggc gatgctctaa atcgtcagcg cgaagccttt atggcccgct
     9361 ggattggatg ggcgatgcag caaaataatg gcgacgtgtt agttgtctgc ggtggctggc
     9421 acgctccggc actggcaaaa atgtggcgcg aatgtccgca ggtaattaac acgccagaat
     9481 tgccgtcgct ggcagatgcc gttacaggtt gttatctcac cccctacagt gaaaagcgcc
     9541 ttgatgtgct ggcaggatac ctttcaggaa tgcctgcccc ggtctggcaa aactggtgct
     9601 ggcagtgggg cttacagcag gcgggagaac aactgctaaa aacggttctc acccgtttgc
     9661 gccagcacaa gttgcctgct tcgaccgcgg atatggctgc cgctcatctg catgcgatgg
     9721 cactggcaca gttgcgcggt catacactac cgttacgcac tgactggctg gatgccatag
     9781 caggatcgct gattaaagaa gccctgaatg cgccgttgcc gtggagctat cgcggcgtta
     9841 ttcatcccga taccgatccg attctgctaa cgttgataga cacattagcg ggtgacggat
     9901 tcggtaaact tgccccttct acgccacaac cgcctctgcc aaaagatgtc acttgcgaac
     9961 tggaacgtac cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg
    10021 gactagcgca aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac
    10081 gccagcaggg aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc
    10141 gcccgcttag ccagcatgcg gcattgattg aggccgcctg ctttggtgcc acactcctgg
    10201 aagccgcacg ccataaatta gaagccgata tgctggacgc gggtggaatc ggcagcatta
    10261 ccacatgtct tagccaagcg gcgtcagcgg gtctggcgtc cttcagtcaa caattactgg
    10321 agcaactcac attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg
    10381 aagtgctata tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat
    10441 tacaaacgac gttatgcgcg gctatcgatc gcacgctgtg gctgtgtgaa tccaacggca
    10501 gaccggatga aaaggagttt cacgctcact tgcatagctg gcaagcgttg tgtcatatcc
    10561 tgcgcgatct acatagcggt gtgcaactcc ccggcgtttc gctttctgcg gcggtagcct
    10621 tgctggagcg acacagtcag gcgattcatg tcctggcact ggatcgcggc gcgactcttg
    10681 gtgcgctgat gcgtctggaa catcccaacg ctagtgccga agcggcgctg acgatgctgg
    10741 cgcagttatc cccggcacaa tccggcgagg cgctgcacgg tttgctggca ttagcccgtc
    10801 atcaactggc ctgtcagccg gcatttatcg ccggtttcag cagtcattta aatcaactaa
    10861 gtgatgccga ttttaccaat gccctgcccg atttacgcgc ggcaatggcc tggctaccgc
    10921 cacgagaacg cgggacgctg gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc
    10981 ccgtttcggc actgcaaatg ccgttgcatt gtccaccgca agccattgca catcatcaac
    11041 aactcgaaca gcaggcactg gcatcgctgc aacactgggg agttttccat gtctgaactg
    11101 aacgatcttc tgaccacccg tgagctacaa cgctggcgat taattcttgg cgaagcggca
    11161 gaaacgacgc tttgtgggct ggatgacaac gcccggcaga tagatcacgc gctggagtgg
    11221 ctgtatgggc gcgatcctga acggctccag cgtggtgaac gctctagtgg attaggtggc
    11281 tcaaatctca ccactcctga gtggatcaac agtattcaca cgctgtttcc gcaacaggtg
    11341 attgagcggc tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacaaat
    11401 ctcgacgtgc tggaacgtat gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc
    11461 aaacatctga tgaaccccga agtactggct gccgcccgca ggattgtgcg ccaggttgtg
    11521 gaagaaatta tggcgcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat
    11581 cgtcgtcgcc gctcatttat tccactggcg cgaaactttg atttcaaaag tactctgcgc
    11641 gccaacctgc aacactggca cccgcaacac ggcaagttgt atatcgaatt cccccgcttt
    11701 aacagccgca ttaaacgcca aagcgagcaa tggcaactgg tcttactggt tgatcaaagc
    11761 ggatcgatgg tcgattcggt gatccactct gctgtgatgg cggcctgttt atggcagtta
    11821 cccggcattc gtacccatct ggtggcgttt gacacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagccac tatcatgccg
    13081 gagaatgctt acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg
    13141 cccggagcga cagtgttggt gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg
    13201 gcattgccgg agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct
    13261 ggcaggatat tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta
    13321 ttttgcacct ttcgcgcagt tggccgcgaa gtgaacagga agactcaccg gcagctacgc
    13381 gtcggctgtt tagttttcag gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag
    13441 caaaacgcag tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg
    13501 taccgctgtc gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg
    13561 tggctttgcg ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg
    13621 tcgataactt atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc
    13681 gccagacgct ggatgcgcag gtaatcagcg gcgaaggtga agataatctg ctgacgttat
    13741 cactgccagc ctcagccagc gcgccttatg ccattgaacg catggcggcg cttttgcaac
    13801 aaacagacga ccccgtgtgt ctggtttctg gctttgtcag ttttgttgat gggcaattga
    13861 cactggaacc acaggtaatg atgacaaaga ctcgtgcctg ggcgctggac gcagaaactg
    13921 cgcctgtggt ggtatcacta ccttctgcca gcgttctgcc tgtgccgtct actgctcatc
    13981 agttgctgat gcgctgccag gcgttactta ttcaactgct ccataacggc tggcgctatc
    14041 aggaacagag cgctattaat caggcagagt tgctggcgaa tgacctcacc gcggtgggtt
    14101 tttatcggct ggcacatgtg ttggcacaat ttcgtaatac agaaagcgag gcacgggtag
    14161 aagcaatgaa taacggtgtt ttgctttgcg aacaattatt ccccatgctt cagcaacaag
    14221 gatgaaatag tgctttttac taagagttct actccagttc cggactgctc acgccacggt
    14281 attaggcata tcctatatag cccctggtga gagtcacc
//
LOCUS       DAZOZX010000099        14168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZOZX010000099 DAZOZX010000000
VERSION     DAZOZX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.099"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..79
                     /locus_tag="ACHUPT_002922"
     CDS             <1..79
                     /locus_tag="ACHUPT_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820420.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUPT_002923"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUPT_002923"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0820421.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHUPT_002924"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHUPT_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0820422.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUPT_002925"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUPT_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0820423.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUPT_002926"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUPT_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0820424.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUPT_002927"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUPT_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0820425.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHUPT_002928"
     CDS             5377..5835
                     /locus_tag="ACHUPT_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0820426.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHUPT_002929"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHUPT_002929"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0820427.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHUPT_002930"
     tRNA            6887..6971
                     /locus_tag="ACHUPT_002930"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHUPT_002931"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHUPT_002931"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUPT_002932"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUPT_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0820428.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUPT_002933"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUPT_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0820429.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUPT_002934"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUPT_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0820430.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHUPT_002935"
     CDS             complement(10252..13995)
                     /locus_tag="ACHUPT_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0820431.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3499 a         3747 c         3401 g         3521 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagca
//
LOCUS       DAZOZX010000100        14110 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZOZX010000100 DAZOZX010000000
VERSION     DAZOZX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.100"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..415
                     /locus_tag="ACHUPT_002936"
                     /pseudo
     CDS             1..415
                     /locus_tag="ACHUPT_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555244.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            580..4698
                     /gene="pic"
                     /locus_tag="ACHUPT_002937"
     CDS             580..4698
                     /gene="pic"
                     /locus_tag="ACHUPT_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR0820432.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4849..5091)
                     /locus_tag="ACHUPT_002938"
                     /pseudo
     CDS             complement(4849..5091)
                     /locus_tag="ACHUPT_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5130..6080)
                     /locus_tag="ACHUPT_002939"
                     /pseudo
     CDS             complement(5130..6080)
                     /locus_tag="ACHUPT_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6131..6706)
                     /locus_tag="ACHUPT_002940"
                     /pseudo
     CDS             complement(6131..6706)
                     /locus_tag="ACHUPT_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6710..7110
                     /locus_tag="ACHUPT_002941"
                     /pseudo
     CDS             6710..7110
                     /locus_tag="ACHUPT_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7513..8094)
                     /locus_tag="ACHUPT_002942"
     CDS             complement(7513..8094)
                     /locus_tag="ACHUPT_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820433.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHUPT_002943"
     CDS             complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHUPT_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0820434.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12359..13363)
                     /locus_tag="ACHUPT_002944"
                     /pseudo
     CDS             complement(12359..13363)
                     /locus_tag="ACHUPT_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13348..13725
                     /locus_tag="ACHUPT_002945"
                     /pseudo
     CDS             13348..13725
                     /locus_tag="ACHUPT_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13764..>14110)
                     /locus_tag="ACHUPT_002946"
     CDS             complement(13764..>14110)
                     /locus_tag="ACHUPT_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0820435.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3541 a         3317 c         3459 g         3793 t
ORIGIN      
        1 catcgtgtgg tgacgagtct catcgtctat ggaaacgaca ttcgtgctgg atgcactgga
       61 gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca
      121 gtatgtgtca ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg
      181 gagtactggt gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc
      241 ggaggtaata caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac
      301 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc
      361 agaagcagaa aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca
      421 cagataaaac actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca
      481 gtaactatat tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt
      541 tacttttata tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt
      601 aaatattgcc ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata
      661 aaaaagacat gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg
      721 tgttactctc agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat
      781 cgtgatttcg ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat
      841 gataaggacg gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt
      901 gtgagctcaa atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat
      961 aacggaggat atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac
     1021 cgtaataacc acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa
     1081 gttattccct cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa
     1141 cgttacaccg ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga
     1201 aatttagtta aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct
     1261 ctgatatctg atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc
     1321 cctttacctg actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa
     1381 caacaaaaaa aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct
     1441 acgaactggt ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc
     1501 gatgcccccg tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca
     1561 tcaggcacag gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac
     1621 aatgacctca atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg
     1681 aaagacagtg tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct
     1741 gctgaatccg gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta
     1801 acctggaagg tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg
     1861 accataaacg gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta
     1921 cttaaccagc aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc
     1981 agcggacgtc cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca
     2041 tggggatacc ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg
     2101 caggctgctg attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta
     2161 ctggatctta aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc
     2221 tttggtggta ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc
     2281 ttctatattc tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat
     2341 ccggctcagt gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat
     2401 aggatcctgg ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat
     2461 atggatgtcg ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg
     2521 aacctgccgg aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg
     2581 gttatccatg cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa
     2641 aaccgtcagt ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt
     2701 aacgcctcgc tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac
     2761 agggcatttg tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc
     2821 tctgtcccgg acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat
     2881 aactcagccc tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc
     2941 gtcagcatca cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca
     3001 ctgacagtgc atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat
     3061 attcaggccg gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct
     3121 aatcagtatg cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca
     3181 gcactggaaa ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca
     3241 acagttacca tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg
     3301 gcgtttgccg gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt
     3361 ggcagggctg atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc
     3421 cacagtctta ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc
     3481 ctgacggtga ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa
     3541 aatgccgata aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc
     3601 agctttatga ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg
     3661 gcgggaactt cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg
     3721 accccaaccc tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt
     3781 aaagcggagg ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat
     3841 aaaaacttca tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca
     3901 aacggtgatg ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt
     3961 tacagtgata attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt
     4021 gtggacctgt ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca
     4081 ttcagcggaa agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc
     4141 ggtgcctata tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac
     4201 tttgctagcc tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg
     4261 ggttaccgct atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac
     4321 ggcgccgtgt ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag
     4381 aacagggact tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt
     4441 ggtaaggact ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat
     4501 aatggagaga ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac
     4561 agccggatgc tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt
     4621 ctggagtttg agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat
     4681 ttccggtata tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga
     4741 ctcttaaaat ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt
     4801 tttgccgact ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc
     4861 gtccaggcgg tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa
     4921 cggcatcgtc catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga
     4981 gtcgtctgtc gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga
     5041 ctcgatggca ttcgtcgtat agatcacttt gcggatgtcc gttggataag cggtaagcgt
     5101 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     5161 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     5221 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgcg
     5281 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
     5341 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
     5401 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
     5461 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
     5521 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5581 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5641 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
     5701 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
     5761 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
     5821 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5881 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5941 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     6001 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     6061 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
     6121 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
     6181 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
     6241 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     6301 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     6361 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     6421 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     6481 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     6541 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     6601 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     6661 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     6721 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     6781 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     6841 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     6901 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga
     6961 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     7021 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     7081 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta
     7141 cgcttactca tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac
     7201 cctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca
     7261 aaataaatca accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct
     7321 gaaaaacgcg tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca
     7381 acacgctcat gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg
     7441 cgtttataca aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata
     7501 ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct
     7561 tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa
     7621 tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat
     7681 gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat
     7741 gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag
     7801 catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat
     7861 gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa
     7921 ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat
     7981 agtttctttt tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa
     8041 aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc
     8101 tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt
     8161 tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac
     8221 cgaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt
     8281 ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg
     8341 caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg
     8401 agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct
     8461 tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat
     8521 aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg
     8581 cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg
     8641 tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag
     8701 agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg
     8761 cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc
     8821 catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc
     8881 caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg
     8941 tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt
     9001 aattaccgga catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat
     9061 atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata
     9121 tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg
     9181 gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga
     9241 ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat
     9301 ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt
     9361 ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc
     9421 gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc
     9481 tggctgaggc tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca
     9541 aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa
     9601 caccaaacag aatagatgct tttttgtctg attttatatt accattgacc caggcataat
     9661 tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac atatagattg
     9721 ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt
     9781 caatagcttt atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa
     9841 tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa
     9901 cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat
     9961 ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga
    10021 acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt
    10081 gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc
    10141 ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg
    10201 acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag
    10261 aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg
    10321 catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt
    10381 tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat
    10441 tgataatatg atttatgctt atattacctg atacattccc atggtacata tatgcatcag
    10501 tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat
    10561 ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt gttccgccat
    10621 ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt
    10681 ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga
    10741 tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac
    10801 cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg
    10861 tattattatc aatatctatc ccggcacctt tataagactt atcttttccg tacactttat
    10921 atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg
    10981 ttaatacccc tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt
    11041 gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa
    11101 tattattaaa taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg
    11161 tactggtaac accatggaaa acccattttt gttgttcttt atcatataga taagaaccag
    11221 aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac
    11281 tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt
    11341 gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga
    11401 agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg
    11461 gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca
    11521 gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat
    11581 cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt
    11641 acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag
    11701 gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac
    11761 ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat
    11821 ttatatccag cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa
    11881 taataccgag gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag
    11941 aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac
    12001 gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca acggggaact
    12061 atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta
    12121 tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg
    12181 atcacttggt gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt
    12241 caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga
    12301 cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc
    12361 agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg
    12421 caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc
    12481 cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat
    12541 taaattctgt gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg
    12601 gggtcagatc cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg
    12661 tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac
    12721 tacgggagat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac
    12781 tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg
    12841 cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt
    12901 tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa
    12961 tacccacatc agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt
    13021 ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga
    13081 ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat
    13141 caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga
    13201 agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga
    13261 tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat
    13321 ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc
    13381 aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta
    13441 atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg
    13501 gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag
    13561 aaagaacaca cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag
    13621 cactgggctg cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca
    13681 gcagaaagag cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat
    13741 tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca
    13801 gcaagtgcaa tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact
    13861 tgtgattttt tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct
    13921 ttccatgcaa tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat
    13981 gctccaactt ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt
    14041 tcgctgtttc caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca
    14101 cctggcacca
//
LOCUS       DAZOZX010000101        13882 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZOZX010000101 DAZOZX010000000
VERSION     DAZOZX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.101"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..197)
                     /locus_tag="ACHUPT_002947"
     CDS             complement(<1..197)
                     /locus_tag="ACHUPT_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0820436.1"
                     /translation="MLARDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEF"
     gene            complement(207..758)
                     /gene="ybcL"
                     /locus_tag="ACHUPT_002948"
     CDS             complement(207..758)
                     /gene="ybcL"
                     /locus_tag="ACHUPT_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0820437.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(927..1259)
                     /gene="emrE"
                     /locus_tag="ACHUPT_002949"
     CDS             complement(927..1259)
                     /gene="emrE"
                     /locus_tag="ACHUPT_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0820438.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1604..1918)
                     /gene="fliE"
                     /locus_tag="ACHUPT_002950"
     CDS             complement(1604..1918)
                     /gene="fliE"
                     /locus_tag="ACHUPT_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0820439.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2132..3791
                     /gene="fliF"
                     /locus_tag="ACHUPT_002951"
                     /pseudo
     CDS             2132..3791
                     /gene="fliF"
                     /locus_tag="ACHUPT_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            3784..4779
                     /gene="fliG"
                     /locus_tag="ACHUPT_002952"
     CDS             3784..4779
                     /gene="fliG"
                     /locus_tag="ACHUPT_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0820440.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4772..5458
                     /gene="fliH"
                     /locus_tag="ACHUPT_002953"
     CDS             4772..5458
                     /gene="fliH"
                     /locus_tag="ACHUPT_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0820441.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5458..6831
                     /gene="fliI"
                     /locus_tag="ACHUPT_002954"
     CDS             5458..6831
                     /gene="fliI"
                     /locus_tag="ACHUPT_002954"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0820442.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6853..7194
                     /gene="fliJ"
                     /locus_tag="ACHUPT_002955"
                     /pseudo
     CDS             6853..7194
                     /gene="fliJ"
                     /locus_tag="ACHUPT_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            7191..8318
                     /locus_tag="ACHUPT_002956"
     CDS             7191..8318
                     /locus_tag="ACHUPT_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0820443.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8423..8887
                     /gene="fliL"
                     /locus_tag="ACHUPT_002957"
     CDS             8423..8887
                     /gene="fliL"
                     /locus_tag="ACHUPT_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0820444.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8892..9896
                     /gene="fliM"
                     /locus_tag="ACHUPT_002958"
     CDS             8892..9896
                     /gene="fliM"
                     /locus_tag="ACHUPT_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0820445.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9893..10306
                     /gene="fliN"
                     /locus_tag="ACHUPT_002959"
     CDS             9893..10306
                     /gene="fliN"
                     /locus_tag="ACHUPT_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0820446.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10309..10674
                     /gene="fliO"
                     /locus_tag="ACHUPT_002960"
     CDS             10309..10674
                     /gene="fliO"
                     /locus_tag="ACHUPT_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0820447.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10674..11439
                     /gene="fliP"
                     /locus_tag="ACHUPT_002961"
                     /pseudo
     CDS             10674..11439
                     /gene="fliP"
                     /locus_tag="ACHUPT_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            11449..11718
                     /gene="fliQ"
                     /locus_tag="ACHUPT_002962"
     CDS             11449..11718
                     /gene="fliQ"
                     /locus_tag="ACHUPT_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0820448.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11727..12512
                     /gene="fliR"
                     /locus_tag="ACHUPT_002963"
     CDS             11727..12512
                     /gene="fliR"
                     /locus_tag="ACHUPT_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0820449.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12797..13421
                     /gene="rcsA"
                     /locus_tag="ACHUPT_002964"
                     /pseudo
     CDS             12797..13421
                     /gene="rcsA"
                     /locus_tag="ACHUPT_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(13465..13653)
                     /gene="dsrB"
                     /locus_tag="ACHUPT_002965"
     CDS             complement(13465..13653)
                     /gene="dsrB"
                     /locus_tag="ACHUPT_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0820450.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13816..>13882
                     /gene="yodD"
                     /locus_tag="ACHUPT_002966"
     CDS             13816..>13882
                     /gene="yodD"
                     /locus_tag="ACHUPT_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodD family peroxide/acid resistance protein"
                     /protein_id="HFR0820451.1"
                     /translation="MKTAKVYSDTAKREVSVDVDAL"
BASE COUNT         3456 a         3577 c         3594 g         3255 t
ORIGIN      
        1 taaaattcat atccacaggg aaataatatt atctgattat tatcaacagt tagactatgc
       61 gtctcccaat tgataacttt ttttccctga cggatacgac ataaagctgg cataagaggc
      121 ttaaccctgt gaatctcatg atgtttatgc atccgtattt cttcaatctt taggttagac
      181 ttatctcttg ccagcatact cttaccctat tttatctcat aaactggtgt tatctcagcg
      241 gttgcaattt tattggcatt aagcatataa ccaactaacg ctccgctgga attagaatcc
      301 acaggaatct tatcagtttt tagagcccat actttaaact ggtaatgatg gggtttatct
      361 cctttgggag gacatgctcc accaaatcct gcataaccaa aatcatttcg gccttgaaca
      421 gcaccagtcg gaagttttgt accatcacgt cttcctgcat cagtgggcaa atatgttaca
      481 gttgctggaa tattagcaac agtccagtgc caccatccac tgcctgtagg agcatctgga
      541 tcatacacag ttacggcaaa gcttttggtg ccttcaggag caccagacca ggttaatgag
      601 ggcgatgtat taccaccttc acacccaaat ccagaaaata catgagacgt tgtaagttgc
      661 tctcctgttt ttatttcatt actagtgacc tgaaatgctg cggcctgcgc agaaaatgtt
      721 atgaatgcca atacacttga aacgataagt tttttcataa aaacctcatt gttgtgacct
      781 atcgttattt tatttgatgt gccttgatct cattatgcat aaaggcgcaa tgatcgcgca
      841 aatgcaatca taattataaa ggtgccccga acattagcca taatatgcac aaatcagatt
      901 cctgttatct caaggagaat tattttttaa tgcggtgcac ttcgtgacaa caagttaatg
      961 actaacacac cggcacaaat caacatcatg cctatgatag ctggcaggtc cagtcgttga
     1021 ccaaaaaatc cccaggaaag caaactaatc agaacgatac cgactcctga ccagatagca
     1081 taagcaatcc ctgtaggtat ataagccagc gtctgagcta ataaccagaa tgatgcacaa
     1141 tagcaaataa ttgtgccaac agagggccat aaccgtgtaa acccctctga aaacttcatt
     1201 aaggttgtac caatgacctc tgcaagtatt gcgccaccaa gataaatata agggttcata
     1261 gcatattctt tcctgttcat actggagaga attgtactac agtttgaaca caactcaccg
     1321 ttttcatcct ggtgtatgtg tatccaaggg gctctggcgt ggcaagagat aaaggcagga
     1381 acaataatga tcagaaggaa ccccaggcta acgaatgagt tcactcttcc tgggagcaaa
     1441 gcgaagacaa aacatgttat cgcgtttacg gatcgtggat ggcagaaaac aaccaggatc
     1501 aggtatccat tttaaaccag aaattgagtg agtttgcccc atccatgccc cacgctgtgg
     1561 gatcggatat aattaaacaa gcatagtcaa caggttacac aacctacacc tgcatggaca
     1621 tcacttcctg atacgccgcc accagcttat tacgcacctg aatccccatt tgcatagaaa
     1681 ctgaggcttt ttgcatatcg gtcatcacat cgtttagcgc cacgccgggt tcaccgaggg
     1741 tgaatttttc tgcctgcgtg cgggcgactg tttgtgtatc gcttatgcga tcgagcgcgg
     1801 cgtgcagctg cccggcaaaa ctaatggtcg gttgcggcag tgattcctgc gcacgcgcac
     1861 tcatcgccgt agcctgtaac tggctgataa ccccttcaat cccctgtatc gctgacattt
     1921 tcgtctcccg gataatttct ggtaacaaag cctaccagta agtcaataag acaaaggcgc
     1981 taaatagcaa caaaaaaact ggtttattgg cggatagaaa aaacgaaagc acaaataatg
     2041 ggagcgtcaa tttttcgagt gtgctgaccc gagagtgagt cttgttccac tttgccaata
     2101 acgccgtcca taatcagcca cgaagtgcgc gatgaatgcg actgcagccc agacaaaatc
     2161 tcttgagtgg cttaatcgcc tgcgtgcgaa tccgaaaatt ccattgattg ttaccggttc
     2221 cgcggcagtg gcggtcatgg tcgcactgat cctgtgggcg aaagcccccg actaccgcac
     2281 attattcagc aatctttccg atcaggatgg tggcgcaatt gtcagccaac tgacgcaaat
     2341 gaatattcct taccgcttca gcgaagccag cggcgctatt gaggttccgg cagataaagt
     2401 tcacgaactg cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact
     2461 gctcgatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc
     2521 gctggaaggc gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt
     2581 acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc
     2641 ggtgacggta aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgctattgt
     2701 acatctggtt tccagcgcgg ttgctggtct gccgcctgga aacgtcacgc tggtggatca
     2761 gggcgggcat ctgttaaccc agtccaatac cagcgggcgc gatcttaatg acgctcagtt
     2821 gaaatatgcc agcgatgtcg aaggccgtat tcagcagcgt attgaagcga tcctgtcacc
     2881 tattgttggt aacggtaata ttcacgccta ggtcacggcg caactggact tcgccagtaa
     2941 agaacaaacg gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc
     3001 acgccagctt aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc
     3061 gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cgacaaatca
     3121 aaataaccgc cagcagcagg cgagcaccac cagcaatagt gggccgcgta gcacacagcg
     3181 gaatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg
     3241 cgatgtgcaa cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa
     3301 accattgcct ctcagcaacg aacagatgaa gcaaattgaa gctctgaccc gcgaggcgat
     3361 gggcttttct gaaaaacgcg gtgattcgct caatgttgtt aactcgccgt tcaatagcag
     3421 tgacgaaagc ggcggagcac tgccgttttg gcaacagcaa gtgtttatcg atcagttgct
     3481 ggctgccggt cgctggttgc tggttgctgg tactgctggt ggcgtggttg ctgtggcgga
     3541 aagcggtacg cccgcagcta acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg
     3601 cgcaggcccg cgaggaagtg gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac
     3661 tccaacaacg gcgcgctaac caacgactgg gggcagaagt catgagccag cgtatccgtg
     3721 aaatgtctga taacgatccg cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg
     3781 atcatgagta acctgacagg caccgataaa agcgtcatcc tgctgatgac cattggcgaa
     3841 gaccgggcgg cagaggtgtt caagcacctc tcccagcgcg aagtgcaaac cctgagcgct
     3901 gcaatggcga acgtcacgca gatctccaac aagcagctaa ccgatgtgct ggcggagttt
     3961 gagcaagaag ctgaacagtt tgctgcactg aatatcaacg ccaacgatta tctgcgttcg
     4021 gtattggtca aagctctggg tgaagaacgt gccgccagcc tgctggaaga tattctcgaa
     4081 acccgcgata ccgccagcgg tattgaaacg ctcaacttta tggagccgca gagcgccgcc
     4141 gatctgattc gcgatgagca tccgcaaatt atcgccacca ttctggtgca tctgaggcgc
     4201 gcccaggccg ccgatattct ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg
     4261 cgtatcgcca cctttggcgg cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg
     4321 aatggcttgc tcgacggtca gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca
     4381 gccgaaatta tcaacctgat gaaaacgcag caggaagaag ccgttattac cgccgtgcgt
     4441 gaattcgacg gcgagctggc gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg
     4501 gtggatgtcg atgatcgcag cattcagcgt ctgttgcagg aagtggattc cgaatcgctg
     4561 ctgatcgcgc tgaaaggggc cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg
     4621 cagcgtgccg ccgatattct gcgcgacgat ctcgccaagc gtggtccggt gcgtctgtcg
     4681 caggtcgaaa acgaacagaa agcgattctg ctgattgtgc gccgtcttgc cgaaactggc
     4741 gagatggtga ttggcagcgg cgaggatacc tatgtctgat aatctgccgt ggaaaacctg
     4801 gacgccggac gatctcgcgc caccaccggc agagtttgtg ccaatggtcg agtcggaaga
     4861 aaccattatt gaagaggccg aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca
     4921 ggcccacgag cagggttatc aggcggggat tgccgaaggt cgccagcaag gtcacgaaca
     4981 gggctatcag gaaggactgg cccaggggct ggaacaaggt ctggcagagg cgaaggctca
     5041 acaagcgcca attcatgccc ggatgcagca actggtcagc gaatttcaaa ctacccttga
     5101 tgcgcttgat agtgtgatag cttcgcgcct gatgcagatg gcgctggagg cagcacgtca
     5161 ggtgatcggt cagacaccaa cggtggataa ctcggcactg atcaaacaga tccaacagtt
     5221 gttgcagcaa gaaccgttat tcagcgggaa accacagctg cgcgtacacc cggatgatct
     5281 gcaacgtgtg gatgatatgc tcggcgcaac tttaagtttg catggctggc gcttgcgggg
     5341 cgatcccacc ctccaccctg gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc
     5401 cagtgtcgcc actcgctggc aagaactctg ccgtctggca gcaccaggag tggtgtaatg
     5461 accacgcgcc tgactcgctg gctaaccacg ctggataact ttgaagccaa aatggcgcag
     5521 ttgcctgcgg tacgtcgcta cgggcgatta acccgcgcta ccgggctggt gctggaagcc
     5581 accggattac aattgccgct cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa
     5641 acgcacgaag tagaaagcga agtcgttggc tttaacggtc aacggctgtt tttaatgccg
     5701 ctggaggaag tggaaggtgt cctgcccggc gcgcgtgtat atgccaaaaa catttcggca
     5761 gaagggctgc aaagcggcaa gcagttgccg ctcggtccgg cgttattagg tcgtgttctg
     5821 gacggtagcg gtaaaccgct cgatggcctg cccttccccg atacgacgga aaccggtgcg
     5881 ctgattaccc cgccatttaa cccgttgcaa cgtacaccga ttgaacatgt gctggacacc
     5941 ggcgtgcgcc caatcaatgc cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt
     6001 gccgggtcgg gcgttggtaa aagtgtgctg ctggggatga tggcacgcta cacccgcgcc
     6061 gatgtcattg tcgtgggttt gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag
     6121 aatatcctcg gtgccgaagg acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt
     6181 tctccgctcc tgcgaatgca aggtgccgcc tatgccacgc gcattgccga agattttcgc
     6241 gatcgtggtc agcatgtgtt gctgattatg gactccctca cccgctacgc gatggcccag
     6301 cgtgaaattg cgctggcgat tggcgaacca cctgccacta aaggttatcc accgtcggtg
     6361 tttgccaaat taccggcact ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg
     6421 attaccgcgt tttataccgt gctcaccgaa ggcgatgacc agcaggaccc cattgctgac
     6481 tccgcgcggg caatcctcga cggtcacatt gtgctgtctc gccgactggc ggaagccggg
     6541 cactatccgg ctatcgatat tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt
     6601 gagcaacatt acgcgcgagt gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac
     6661 cgcgatctgg ttagcgtcgg cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc
     6721 atcgccctgt ggccgcagct ggagggctat ttgcaacaag gcatttttga acgcgcggac
     6781 tgggaagcgt ctctccaggg gctggagcgt attttcccga cagtgtcata acccaggaga
     6841 taacggcaga tggcggaaga acagctcaaa atgttgatcg attatcagaa tgaatatcgc
     6901 aataacctca atagcgatat gagtgccggg atgaccagca accgctggat caactatcag
     6961 cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact taatcagtgg
     7021 acgcagaaag ttgacattgc cctgaacagc tggcgagaaa agaaacaacg tttgcaggcc
     7081 tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa ccgcctcgat
     7141 cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga atgattcgct
     7201 tagcgccctt gattaccgcc gacgttgaca ccaccacatt gcctggcggc aaagccagcg
     7261 atgctgcaca agattttctc acgttgttga gcgaagcatt agcaggcgag acaacaactg
     7321 acaaagcggc cccccagttg ctggtagcaa cagataagcc cacgacaaaa ggcgagccgc
     7381 tggtcagcga tattcttgcc gatgcgcaac aagcggattt actgatccct gtggatgaaa
     7441 cacttcctgt catcaacgac gaacaatcca catcaacacc gttaaccacc gctcagacga
     7501 tgacgttggc tgcggtggct gacaaaaata cgacaaaaga cgaaaaagcg gatgatctta
     7561 atgaagacgt caccgcaagt ctgagcgccc tttttgcgat gttgccgggt tttgacaata
     7621 cgcccaaagt gactgatgca ccgtcaaccg tgttaccggc agagaaacca acgctcttca
     7681 caaaactgac ttctgcgcaa ctcactacag cacagcctga tgacgccccc ggcacaccag
     7741 ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc atcagcacgc
     7801 cttcgccggt gaccgctgac gccagcccgc taatcactcc acaccagaca cagccactgc
     7861 ccaccgtcgc cgcgcctgtt ttgagtgcac cgctgggttc tcacgaatgg caacaatcat
     7921 tgagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag ttgcgtctgc
     7981 acccacagga tttaggtgaa gtgcaaattt ccctcaaagt ggatgataac caggcgcaga
     8041 tccagatgat ttcaccgcat cagcacgtac gcgccgccct ggaagcagcg ctgccggtac
     8101 tgcgtacgca gctggccgaa agtggcattc agttagggca aagcaacatt agtggcgaaa
     8161 gctttagtgg tcagcagcag gccgcttccc agcaacagca aagccaacgc acagcaaacc
     8221 atgaacctct ggcgggggaa gacgacgata cgcttccggt tcccgtctct ttacaagggc
     8281 gtgtaacagg taacagcggc gttgatattt tcgcctaacg tcagaggtag caccgtaatc
     8341 cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga taagcagtag
     8401 cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa gcaagcgatc
     8461 gctttggatc ccgattctgg tattcattac cctcgcggcc tgtgccagcg caggttacag
     8521 ctactggcat tcgcatcagg ttgccgctga cgacaaagcg cagcaacgcg tcgtgccctc
     8581 cccggtcttc tatgcgctgg atacctttac ggtcaatttg ggcgatgcgg atcgcgtgct
     8641 ttatatcggc ataaccctgc gactgaaaga tgaagcaacc cgctcgcggc tgagtgagta
     8701 tttgccggaa gtccgtagtc gcttgctgtt actgttttca cgtcaggatg ctgccgtact
     8761 ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt aaaaccacac tttccacccc
     8821 gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg cttttattct
     8881 gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg ctgttgaatg
     8941 gtgacagcga agtcaaagac gaaccgacag ccagtattag cggcgaaagt gacattcgtc
     9001 cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg ctggaaatca
     9061 ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg cgccgtagcc
     9121 cggacattac cgtcggggcc atccgcattc agccgtacca tgaatttgcc cgcaacctgc
     9181 cggtgccgac caacctgaac ctaatccacc tgaaaccgct acgcggcaca gggctggtag
     9241 tgttctcgcc gagtctggtg tttatcgccg tggataacct gtttggcggc gatggacgct
     9301 tcccgaccaa agtggaaggt cgcgagttta cccatactga acagcgcgtc atcaaccgta
     9361 tgttgaagct ggcgctggaa ggctatagcg acgcctggaa agcgattaat ccgctggaag
     9421 ttgagtacgt gcgttcggaa atgcaggtgg aatttaccaa tatcaccacc tcgccgaacg
     9481 acattgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc gaatttaata
     9541 tctgcctgcc attcagcatg atcgagccgc tacgggaatt gttggttaac ccgccgctgg
     9601 aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag gtgcagcatt
     9661 cacagctgga actggtggcc aactttgccg atatctcgct acgcctgtcg cagattttaa
     9721 aactgaaacc cggcgacgtc ctgccgatag aaaaacccga tcgcatcatc gcccatgttg
     9781 acggcgtccc ggttctgacc agtcagtacg gtactctcaa cggtcagtat gcgttacgga
     9841 tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc aaatgagtga
     9901 catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg ctgaagcgtt
     9961 gagcgagcaa aaatcaacca gcgaaaaaag cgctgccgac gcggtgttcc agcaatttgg
    10021 cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata ttccggtcaa
    10081 gctgaccgtc gagctgggcc gtacgcggat gaccatcaaa gagctgttgc gtctgacgca
    10141 agggtcagtc gtggcgctgg acggtctggc gggcgaaccg ctggatattc tgatcaacgg
    10201 ttatttaatc gcccagggcg aagtggtggt tgttgccgat aaatatggcg tgcggatcac
    10261 cgatatcatt accccgtccg agcggatgcg ccgcctgagc cgttagtgat gaataaccat
    10321 gctactgtgc aatcttccgc gccggtttct gctgagccac tgctgcaggt gagcggcgca
    10381 ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg gctgggattt
    10441 gcccctaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc gctgggcgcg
    10501 cgtgaacggg tcgtggtggt cgatgtggaa gatgcacggc tggtgctcgg cgttaccgca
    10561 gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga gataccgcag
    10621 accgattttc agtcggtcat gaaaaatttg cttaagcgtg gcgggagatc ctgatgcgtc
    10681 gtttgttttc tgtcgcaccg gtccttctct ggctgattac gcccctcgcc ttcgcgcaac
    10741 tgccgggtat caccagccag ccgctacctg gcgggtatca ccagccagcc gctacctggc
    10801 ggtggacaaa gctggtcgct cccggtgcag acgctggtgt tcatcacctc gttgacgttc
    10861 attccagcga ttttactgat gatgaccagt ttcacccgca tcattattgt ttttggttta
    10921 ttgcgtaacg cgctgggaac gccctccgcg ccacctaacc aggtattgct ggggctggca
    10981 ctgtttttga ccttttttat tatgtcgcca gtgatcgaca aaatttatgt cgatgcgtac
    11041 cagccattca gcgaagagaa aatctcaatg caggaggcgc tggaaaaagg ggcgcagccg
    11101 ctgcgtgagt ttatgctgcg tcagacccgc gaggcagatt tagggttgtt tgccagactg
    11161 gcgaataccg gcccgttgca ggggcctgaa gctgtgccga tgcgcatttt gctcccggcc
    11221 tacgtgacca gcgagttgaa aaccgcattt cagataggct tcacgatttt catccctttt
    11281 ttgattatcg acctggtgat tgccagcgtg ttgatggcat tggggatgat gatggttccc
    11341 cccgccacca ttgctctgcc ctttaaactg atgctgtttg tactggtgga tggctggcaa
    11401 ttgctggtcg gttcgctggc gcagagcttt tacagctaga gaggcaaaat gacacctgaa
    11461 tcggtcatga tgatgggaac tgaagcgatg aaagtcgcgc tggcactggc agccccgctg
    11521 ttgctggtag cgctggtcac gggccttatc atcagtattt tgcaggcagc cacgcagatt
    11581 aacgaaatga cgctgtcgtt tattccgaaa atcatcgccg tatttatcgc catcattatt
    11641 gccggaccgt ggatgctcaa tctgttgctg gattacgtcc gcaccttgtt cactaacctg
    11701 ccgtatatca tcgggtagcc cgtacaatga tgcaggagac aagcgatcaa tggctttcct
    11761 ggttaagcct gtacttctgg ccgttactgc gcgtgctggc gctcatttct accgcgccaa
    11821 ttctgagcga acgcagcgtg ccgaaacggg taaaactggg tctggcaatg atgatcacgt
    11881 tcgccattgc cccatcatta cctgccaacg atgttcctgt tttttcgttc tttgctctgt
    11941 ggctggccgt acagcagatc ctgatcggca ttgcgcttgg ttttaccatg caatttgcct
    12001 ttgccgctgt gcgaaccgct ggcgaaatta tcggtctgca aatggggctg tcatttgcga
    12061 catttgtcga tcctggcagc catctcaata tgcccgtttt agcgcgcatc atggatatgc
    12121 tggcattact gctgttcctg acatttaacg gtcatttatg gttgatttca ctgctggtcg
    12181 atacctttca caccctgccg attggtagcg aaccgttgaa cagcaatgcg tttctggcac
    12241 ccaccaaagc agggagtttg attttcctta acgggctgat gctggcgtta ccgctcatta
    12301 ctctgttgct gaccctgaat ctggcattag gtttactgaa tcgtatggcc ccgcaattat
    12361 ccatttttgt tattggattt ccattaactc tgactgtcgg catctcttta atggcggcat
    12421 taatgccgtt aattgcgcct ttttgcgaac atttattcag tgaaattttt aatttgctgg
    12481 ctgatattat tagtgaatta ccattaatat aattccgtaa cgtttatcat gctaccctaa
    12541 ggattatccg aaaaataata tctgcgaaca tcttccagga tactcctgta gcgaaatatt
    12601 tgttttaagc tcactcacat atcgcaacat ttactttaag acaattccag gcaaattata
    12661 caacacttta cgggatagta agtccgcctg aaaaattgcg agagtggcgc attaggtgac
    12721 ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat
    12781 tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg
    12841 tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac
    12901 cgttgatgac cttgccatag cttgtgattc acagcgtcct tcagtggtgt ttattaatga
    12961 ggactgtttc atccacgatg cttctaacag tcagcgcatc aagcacatca ttaatcaaca
    13021 tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct
    13081 attggtcaga aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg
    13141 atatccttgg cgatattctg aaaaaagaga caacgataac ctcgttttta aatatgccga
    13201 cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca ggtcagggaa
    13261 ccattcaaat ctccgaccaa atgaatatca aagccaagac cgtttcatcg cataaaggta
    13321 atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc gtccgactga
    13381 cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg actggtggtt
    13441 tcccaccagt caggctgaat aagattactc tgctttctca acaaagatac cgtcctgatg
    13501 ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgcaa ccaggtacat
    13561 tgtgccttca ctaaactcct caactgccag taccactcca ggacgacgcg gaccgccatc
    13621 cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc tttgtgccag
    13681 tgtagaacaa tttcgttttt ctggcagcgc caagcgcgcg cgagtgctga ttttctcgac
    13741 ggtctatact taagagatgc caggcggact taacgactgg cggcaacaac agagtaacgg
    13801 ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg ataccgcaaa acgtgaggtc
    13861 agcgtcgatg tcgatgccct gc
//
LOCUS       DAZOZX010000102        13858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZOZX010000102 DAZOZX010000000
VERSION     DAZOZX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.102"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACHUPT_002967"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACHUPT_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0820452.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /locus_tag="ACHUPT_002968"
     CDS             321..1952
                     /locus_tag="ACHUPT_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0820453.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="ACHUPT_002969"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="ACHUPT_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0820454.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="ACHUPT_002970"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="ACHUPT_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0820455.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="ACHUPT_002971"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="ACHUPT_002971"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0820456.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="ACHUPT_002972"
     CDS             6426..7055
                     /locus_tag="ACHUPT_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0820457.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACHUPT_002973"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACHUPT_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR0820458.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="ACHUPT_002974"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="ACHUPT_002974"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0820459.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="ACHUPT_002975"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="ACHUPT_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0820460.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="ACHUPT_002976"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="ACHUPT_002976"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0820461.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3616 a         3592 c         3362 g         3288 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaagctg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggcccca
//
LOCUS       DAZOZX010000103        13248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZOZX010000103 DAZOZX010000000
VERSION     DAZOZX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.103"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUPT_002977"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUPT_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUPT_002978"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUPT_002978"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0820462.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHUPT_002979"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHUPT_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHUPT_002980"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHUPT_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR0820463.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHUPT_002981"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHUPT_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0820464.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHUPT_002982"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHUPT_002982"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0820465.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHUPT_002983"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHUPT_002983"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0820466.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHUPT_002984"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHUPT_002984"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0820467.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHUPT_002985"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHUPT_002985"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0820468.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="ACHUPT_002986"
     CDS             9656..10102
                     /locus_tag="ACHUPT_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0820469.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="ACHUPT_002987"
     CDS             10113..11564
                     /locus_tag="ACHUPT_002987"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR0820470.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="ACHUPT_002988"
     CDS             11554..12624
                     /locus_tag="ACHUPT_002988"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0820471.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="ACHUPT_002989"
     CDS             12624..>13248
                     /locus_tag="ACHUPT_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR0820472.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3159 a         3458 c         3490 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaataatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAZOZX010000104        13161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZOZX010000104 DAZOZX010000000
VERSION     DAZOZX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.104"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUPT_002990"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUPT_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0820473.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHUPT_002991"
     CDS             1131..1931
                     /locus_tag="ACHUPT_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0820474.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHUPT_002992"
     CDS             2009..2779
                     /locus_tag="ACHUPT_002992"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0820475.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUPT_002993"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUPT_002993"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0820476.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHUPT_002994"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHUPT_002994"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0820477.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACHUPT_002995"
     CDS             5034..5756
                     /locus_tag="ACHUPT_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0820478.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHUPT_002996"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHUPT_002996"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0820479.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHUPT_002997"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHUPT_002997"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0820480.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACHUPT_002998"
     tRNA            7149..7225
                     /locus_tag="ACHUPT_002998"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACHUPT_002999"
     CDS             complement(7356..8402)
                     /locus_tag="ACHUPT_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR0820481.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHUPT_003000"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHUPT_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHUPT_003001"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHUPT_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0820482.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHUPT_003002"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHUPT_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0820483.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACHUPT_003003"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACHUPT_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHUPT_003004"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHUPT_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3027 a         3409 c         3493 g         3232 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgtat agactggccc cctgaatctc cagacaacca gtatcactta
    13141 tttaagtgat agtcttaata c
//
LOCUS       DAZOZX010000105        13125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZOZX010000105 DAZOZX010000000
VERSION     DAZOZX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.105"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(109..429)
                     /locus_tag="ACHUPT_003005"
                     /pseudo
     CDS             complement(109..429)
                     /locus_tag="ACHUPT_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(635..1354)
                     /locus_tag="ACHUPT_003006"
                     /pseudo
     CDS             complement(635..1354)
                     /locus_tag="ACHUPT_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACHUPT_003007"
     CDS             complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACHUPT_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0820484.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACHUPT_003008"
     CDS             complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACHUPT_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0820485.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3265..3948
                     /gene="cusR"
                     /locus_tag="ACHUPT_003009"
     CDS             3265..3948
                     /gene="cusR"
                     /locus_tag="ACHUPT_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0820486.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3938..5380
                     /gene="cusS"
                     /locus_tag="ACHUPT_003010"
     CDS             3938..5380
                     /gene="cusS"
                     /locus_tag="ACHUPT_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0820487.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5530..7767
                     /gene="nrfB"
                     /locus_tag="ACHUPT_003011"
     CDS             5530..7767
                     /gene="nrfB"
                     /locus_tag="ACHUPT_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR0820488.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7754..10722
                     /gene="nfrA"
                     /locus_tag="ACHUPT_003012"
                     /pseudo
     CDS             7754..10722
                     /gene="nfrA"
                     /locus_tag="ACHUPT_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10723..11613
                     /locus_tag="ACHUPT_003013"
     CDS             10723..11613
                     /locus_tag="ACHUPT_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0820489.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11796..12557
                     /gene="envY"
                     /locus_tag="ACHUPT_003014"
     CDS             11796..12557
                     /gene="envY"
                     /locus_tag="ACHUPT_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0820490.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12603..12679)
                     /locus_tag="ACHUPT_003015"
     tRNA            complement(12603..12679)
                     /locus_tag="ACHUPT_003015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12643..12645),aa:Arg,seq:tct)
     gene            12922..>13125
                     /locus_tag="ACHUPT_003016"
     CDS             12922..>13125
                     /locus_tag="ACHUPT_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR0820491.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3219 a         3405 c         3330 g         3171 t
ORIGIN      
        1 atccctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgtcgaacca
      121 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
      181 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      241 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      301 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      361 agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      421 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      481 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      541 caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      601 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      661 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      721 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      781 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      841 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      901 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      961 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
     1021 tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
     1081 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1141 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1201 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1261 cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1321 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1381 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1441 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1501 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1561 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1621 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1681 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1741 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1801 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1861 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1921 acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1981 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     2041 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     2101 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2161 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2221 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2281 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2341 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2401 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2461 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2521 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2581 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2641 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2701 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2761 gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc
     2821 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2881 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2941 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     3001 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     3061 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3121 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3181 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3241 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3301 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3361 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3421 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3481 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3541 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3601 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3661 ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
     3721 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3781 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3841 gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3901 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3961 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca tcgcggcgtt
     4021 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     4081 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4141 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4201 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4261 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4321 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4381 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4441 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4501 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4561 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4621 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4681 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4741 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4801 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4861 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4921 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4981 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     5041 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     5101 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5161 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5221 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5281 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5341 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5401 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5461 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5521 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5581 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5641 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5701 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5761 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5821 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5881 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5941 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     6001 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     6061 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6121 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6181 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6241 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6301 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6361 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6421 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6481 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6541 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6601 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6661 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttt
     6721 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6781 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6841 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6901 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6961 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     7021 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     7081 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7141 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7201 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7261 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7321 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7381 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7441 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7501 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7561 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7621 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7681 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7741 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7801 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7861 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7921 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7981 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     8041 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     8101 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8161 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8221 cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8281 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8341 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8401 attaagcgcg gcagaacacc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8461 tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat atattactta
     8521 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8581 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8641 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8701 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8761 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8821 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8881 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8941 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     9001 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     9061 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9121 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9181 ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9241 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9301 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9361 gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca acaccgctca
     9421 ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9481 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9541 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9601 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9661 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9721 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9781 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9841 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9901 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9961 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
    10021 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
    10081 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10141 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10201 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10261 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10321 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10381 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10441 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10501 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10561 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10621 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10681 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10741 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10801 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10861 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10921 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10981 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    11041 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    11101 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11161 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11221 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11281 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11341 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11401 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11461 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11521 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11581 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11641 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11701 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11761 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11821 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11881 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11941 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    12001 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc ccggttatcg
    12061 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12121 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12181 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12241 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12301 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12361 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12421 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12481 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgac
    12541 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12601 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12661 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12721 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12781 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12841 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12901 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12961 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    13021 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    13081 attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAZOZX010000106        13109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZOZX010000106 DAZOZX010000000
VERSION     DAZOZX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.106"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            212..862
                     /gene="csgD"
                     /locus_tag="ACHUPT_003017"
     CDS             212..862
                     /gene="csgD"
                     /locus_tag="ACHUPT_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0820492.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            867..1256
                     /gene="csgE"
                     /locus_tag="ACHUPT_003018"
     CDS             867..1256
                     /gene="csgE"
                     /locus_tag="ACHUPT_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0820493.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1281..1697
                     /gene="csgF"
                     /locus_tag="ACHUPT_003019"
     CDS             1281..1697
                     /gene="csgF"
                     /locus_tag="ACHUPT_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0820494.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1724..2499
                     /locus_tag="ACHUPT_003020"
                     /pseudo
     CDS             1724..2499
                     /locus_tag="ACHUPT_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2563..3054)
                     /locus_tag="ACHUPT_003021"
     CDS             complement(2563..3054)
                     /locus_tag="ACHUPT_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0820495.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3156..3710)
                     /gene="ycdY"
                     /locus_tag="ACHUPT_003022"
     CDS             complement(3156..3710)
                     /gene="ycdY"
                     /locus_tag="ACHUPT_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0820496.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3734..4471)
                     /gene="ycdX"
                     /locus_tag="ACHUPT_003023"
     CDS             complement(3734..4471)
                     /gene="ycdX"
                     /locus_tag="ACHUPT_003023"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0820497.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPCRLLNFLESRGMAPIAEFADL"
     gene            complement(4526..5464)
                     /gene="ghrA"
                     /locus_tag="ACHUPT_003024"
                     /pseudo
     CDS             complement(4526..5464)
                     /gene="ghrA"
                     /locus_tag="ACHUPT_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5699..5786
                     /locus_tag="ACHUPT_003025"
     tRNA            5699..5786
                     /locus_tag="ACHUPT_003025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5733..5735,aa:Ser,seq:gga)
     gene            complement(5936..6217)
                     /locus_tag="ACHUPT_003026"
                     /pseudo
     CDS             complement(5936..6217)
                     /locus_tag="ACHUPT_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6499..7503)
                     /locus_tag="ACHUPT_003027"
     CDS             complement(6499..7503)
                     /locus_tag="ACHUPT_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR0820498.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7575..8135)
                     /locus_tag="ACHUPT_003028"
                     /pseudo
     CDS             complement(7575..8135)
                     /locus_tag="ACHUPT_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8220..8417)
                     /locus_tag="ACHUPT_003029"
     CDS             complement(8220..8417)
                     /locus_tag="ACHUPT_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR0820499.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8429..8920)
                     /locus_tag="ACHUPT_003030"
     CDS             complement(8429..8920)
                     /locus_tag="ACHUPT_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR0820500.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(8917..9291)
                     /locus_tag="ACHUPT_003031"
     CDS             complement(8917..9291)
                     /locus_tag="ACHUPT_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR0820501.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9338..9712)
                     /locus_tag="ACHUPT_003032"
     CDS             complement(9338..9712)
                     /locus_tag="ACHUPT_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR0820502.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(9875..10096)
                     /locus_tag="ACHUPT_003033"
     CDS             complement(9875..10096)
                     /locus_tag="ACHUPT_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR0820503.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10159..10635)
                     /locus_tag="ACHUPT_003034"
     CDS             complement(10159..10635)
                     /locus_tag="ACHUPT_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR0820504.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10651..11124)
                     /locus_tag="ACHUPT_003035"
     CDS             complement(10651..11124)
                     /locus_tag="ACHUPT_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0820505.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11466..12287)
                     /locus_tag="ACHUPT_003036"
     CDS             complement(11466..12287)
                     /locus_tag="ACHUPT_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR0820506.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12387..12560)
                     /locus_tag="ACHUPT_003037"
     CDS             complement(12387..12560)
                     /locus_tag="ACHUPT_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR0820507.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(12680..>13109)
                     /locus_tag="ACHUPT_003038"
     CDS             complement(12680..>13109)
                     /locus_tag="ACHUPT_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820508.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDLGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3316 a         3236 c         3219 g         3338 t
ORIGIN      
        1 aatgagttac attttgttac atgtttaaca cttgatttaa gatttgtaat ggctagattg
       61 aaatcagatg taatccatta gttttatatt ttacccattt agggctgatt tattactaca
      121 cacagcagtg caacatctgt cagtacttct ggtgcttcta ttttagaggc agctgtcagg
      181 tgtgcgatca ataaaaaagt ggggtttcat catgtttaat gaagtccata gtattcatgg
      241 tcatacatta ttgttgatca ctaaaccttc tttgcaggcg acagctctct tgcagcacct
      301 taaacaatcg ctagcaatta caggaaaatt acataatatt caacgttctc tggacgatat
      361 ctcttcaggc tctattattc ttctggatat gatggaagcg gataaaaagc ttatccatta
      421 ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc taaatacgcc
      481 tgaagattac ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg ttttttatgc
      541 catggaggat caagaacgtg ttgtcaatgg gttgcaaggc gtcctgcgtg gcgaatgcta
      601 ctttacgcaa aagcttgcca gttacctgat tacacattct ggtaactatc gttataacag
      661 cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataagc tgcgtatcgg
      721 cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg ttaaaacgca
      781 tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg tttcctgggc
      841 aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt gtggcggcag
      901 aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg ggattgttaa
      961 ctgaccatac tgtttcatct attggccatg atttttaccg agcctttagt gataaatggg
     1021 aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga tggggaagct
     1081 ggatcacaat aacggtcaat caggatgtta ttttccagac ttttttattc ccgttgaaaa
     1141 gagactccga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca ctaaatcgtc
     1201 gccagataaa tcaggcgtta ttaagtacgg acgatttggc gcatgatgaa ttctaaataa
     1261 aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc atgcttattt
     1321 cgccattaag ttgggctgga accatgactt tccagttccg taatccaaac tttggtggta
     1381 acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct tataaagatc
     1441 cgagctataa cgatgacttt ggtattgaaa caccctcagc gttagataac tttactcagg
     1501 ccatccagtc acaaatttta ggtgggctac tgtcgaatat taataccggt aaaccgggcc
     1561 gcatggtgac caacgattat attgttgata ttgctaaccg cgatggtcaa ttgcagttga
     1621 acgtgacaga tcgtaaaacc ggacaaacct cgaccatcca ggtttcgggt ttacaaaata
     1681 actcaaccga tttttaagcc ccagcttcat aaggaaaata atcatgcagc gcttatttct
     1741 tttggttgcc gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag aagccgccag
     1801 accgacatta atgcctcgtg ctcagagcta caaagatttg acccatctgc cagcgccgac
     1861 gggtaagatc tttgtttcgg tatacaacat tcaggacgaa accgggcaat ttaaacccta
     1921 cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc agggcttaca aaacctgctt
     1981 aacgagcgca aggttattcg tgcggcacaa gaaaacggca cggttgccat taataaccga
     2041 atcccgctgc aatctttaac ggcggcaaat attatggttg aaggttcgat tatcggttat
     2101 gaaagcaacg ttaaatcggg cggtgttggg gcaagatatt ttgggatcgg tgcagacacg
     2161 caataccagc tcgatcagat tgccgtgaac ctgcgtgtcg tcaatgtgag taccggtgag
     2221 atcctctcct cggtgaacac cagtaagacg atactttcct atgaagttca ggccggggtt
     2281 ttccgcttta ttgactacca gcgcttgctg gaaggggaag tgggttacac ctcaaacgaa
     2341 cccgttatgc tgtgtctgat gtcggctatc gaaacagggg tcattttcct gattaatgat
     2401 ggtatcgacc gtggtctgtg ggatttgtaa aataaagcag aacggcagaa tgacattctg
     2461 gtgaaatacc gccatatgtc ggttccaccg gaatcctgac agataaaaat gccggaatta
     2521 ataatccggc atttactgtt taagcgaaag cgtgagtgcc cctcacgctt tatttttgat
     2581 ttcctgctca cggtgagccg tctttgcact acgcgccgcc agccacaggc cgctgttttt
     2641 cattgcgtaa ccaaatacca gcccaagcgc cagcgaaggt aacaccagtt tccagtcacc
     2701 ctgcccggca aacgtcgcgc atgcgcctat aaacgtcccc ggaacaaatg aaagcagcag
     2761 ctgtttggcc tgaatacaca tcagaaacgc gacaataccg gttatgacat aaccgagaat
     2821 ttccagatgt ggtgccagtg cactaccgta aataatgacc atcgcccaca ccacgccact
     2881 aagcagggtt gcagcggaga tcgccagccc tttcagccca ccttgcgggc aagcaaagta
     2941 ggccgtacag cccaggaagc ccgcccagct tagtaagccc agggaaacag ccacccatcc
     3001 ccagataccg gagagaatgc ctgttgtgat tgcaatagag agaagtatgt tcatgccgcg
     3061 catgatagca gaaaacgcgg ccatgaatga gatcgagcac acattttatg caacaacaaa
     3121 ttatctgttg cagttatgag tgacgatgat cacatttact cttcagaatc ttcttccagc
     3181 tcgtcccaca ttgcactaat ggcatcacgg gttagcggtg ccatggtgcg ccagaaaggc
     3241 gtggttgcat gggcctccac tttgccgagg aacgcaccac accagggtaa cagatactca
     3301 ctgaacagtg tttccagtgc ttcgctctca tcttccgttg actgatcttc cagccaggaa
     3361 gccgcaagca gcaatgtgcc gatgtgatcg gctggcgtat ccgccagtag catccctcgt
     3421 tcggaaagaa aagcgcgcac ttccgcttcc gttgcgtcct caacccatgc gctacgatat
     3481 ggcggcacgg cacattcatc gccgataaac aacgcattgt aatcggcaga gacttgcgtc
     3541 atatcacagc ttttctgtaa acgcgtcagt aactcatcct gctccagtgg ccagttcgca
     3601 gccagtttcc cctcacgaat cagagtaaac agcggcacca gtaaaggatc ttgcggttgg
     3661 cggtaataga gcgaacccag cacacgacag aggatagaaa actcgttcat taatatattt
     3721 ccattactag taattaaagg tctgcaaatt ccgcaatcgg tgccataccg cgagattcaa
     3781 ggaagttcag taagcggcac ggagaaacat tcaaaatgcg ctctagcgga aaatctaccg
     3841 cgtcgaggat tttaagacac tcttcaaatt cccccatggt aaacgcggtg tgagaatccg
     3901 agcctaatgc cacccaacct ccagcatcac gtaccgctgc agctaccgca cgacagttgt
     3961 cttcactgcc cttacgtgag tgtaaaaatg aggaattatt gatttccagc gccacctgat
     4021 gtttcgcggc tgcttcagca acggctttaa catctatttc atatttcgga tttccgggat
     4081 ggcttattat atgcacattg ccgctggcga tagtggagat catcgcttgt gtattggtcg
     4141 ctttgtcatg tggcgcaaaa accggctcat gaaaaccggc aataatcaga tccagcgagt
     4201 caaacatttt accgctgcag tcaatttcac catcaacatt tttaatgtta gcttcaatgc
     4261 cgcgcaggat ccctacccca tcaaccactc gcggccagat acgcatgtta atgaagtgcc
     4321 agtgatgcgg cgcatcttcc atatccgggc catgatcggt gatcgcaaaa agtttaatgc
     4381 ccttttgttt ggcctgggca atgtaatcac tcaatgtgct atatgcatgt gtgctggcaa
     4441 cggtatgcat atgaaggtcg acgggataca taactctctc ctgtattcaa ttcccgccaa
     4501 ggatagcagg aatcctgatg ctttattagt agccacgtgc gcggtcgact tgcccgcaga
     4561 ccctctcccc tttttcgagc tgggcaatag tgcgagaaat gtactccaca gcttcagcgg
     4621 gacgggtaat cgcggcgaca tgtggtgtta tcgtcacgcg tggatgttgc cagagcggac
     4681 tttcaggcgg taagggttca cgattaaaaa catccagcat tgcgccttta actttcccgc
     4741 tatccagcgc cgcgagcagg tcatcttcca caacatgaac accacgcgcc aggttgagga
     4801 gatacgcgcc atcaggtaat ttttcgagta attgttgatt aataatgccg acggtttcag
     4861 gggtattcgg taacaaatta atcaataccc gacattggct cagaaatgca gacagttctt
     4921 cccgtccggc aaagctttgc acgccaggcc acgatttacg ggttcgactc cagcaacgca
     4981 gcggaaagcg ctaggtttgc agactctgag caactttaca gcccagtacg cctgcgccca
     5041 aaatgccgat ggtaaaatct tcccgatgat attcaggcag cggttgccaa tgcgaactat
     5101 tttgctggat gcgataatcg tcaaaacgtc gaaaccaatg cagcacctga ctgacagcat
     5161 attcctgcat ttgctcgccc ataccggtat cttccaggcg aaaaagtgga acagaagggt
     5221 tcagcatttc agggtgtgcc tgtagcttgc tcaaaataga atcaacaccg gccccgagtg
     5281 cgaacaccgc tttaagatcg cgccctgcca gcatttcaac aggaggatgc cagactaaaa
     5341 cataatcagc agagtcatta tctccgcttt tccatgccct gactcttgcc tgaggaatag
     5401 ctttgcgcag tgcctcaatc caccattggg tatcgaacgt tgggtgataa aagatgatat
     5461 ccatactgac tcccgaaaag cgttgtgcga aatttattcg cacttatcgt tatgatctac
     5521 aaaggccacc agcataacaa atccgtggtc ggtggcaaaa aaagcagatt tcgcttatta
     5581 aaaccacaca ttgattgaat gtttgaataa acgcgcgatt ttttcaaaaa gtttgttgac
     5641 ctcaggtcat gatttcccta aattagcgcc cgttccagca agacaggaac gacaatttgg
     5701 tgaggtgtcc gagtggctga aggagcacgc ctggaaagtg tgtatacggc aacgtatcgg
     5761 gggttcgaat ccccccctca ccgccagatt ataagtgcat gttattgttt aacatgcgct
     5821 tttttatttt tccttccaga cagtatgcca tcgggtatgc catctgaaac cggtttatat
     5881 gccccgcgat catatcttat cggacaattt ccgatctttg agcatactgg caaagctata
     5941 ctgccagcat atcaggatag tgttttgcga taatatcatt cagtctgtca accagttctg
     6001 gttttctgaa cgtgatatgc gcggagccct tcttaaagta tctgatgctg aacatctcat
     6061 cttcatagct ctctttaccc tgaacagact gaatgtgatc atccagacga agggtgatgt
     6121 ttccccggtt gtcggggatt ggtttaccgc tgaacaggtg caacattctt tccagatcgg
     6181 cgagtcggtc agcctgttgt ccggtgataa gatgaaatct atggtcaccc ccgtttttgc
     6241 aacactgatt tttgattaat ggttggcttg cttaaatcta tccggcgtct ctgtgggcaa
     6301 ggatgcccgc gccccgatga gttccgcacc tgataatcct aaaaatcccc tcggcttcag
     6361 tgagccattt catctgttag gtttttaatc aggtcgattt gccgttcatg tcatcaataa
     6421 tcagtcttcg caaaaccaga aaggtaactt gtatcttatt gtttaaaata aactaattga
     6481 gttatatcag catctatatc aggcctgata ttcttttttc tgagtagtga ttgcccaggc
     6541 aattcgagcc agtttatttg ccagagcgca ggtaaccaca ttcgaatgct tcttgttgag
     6601 ttgctcccga acccactcag caagttttcc atgcttgcgt tcaagtcgca ttatgaatga
     6661 gcgggcacac tgaaccagta accggcgtaa atttttatca cctcgcttac tgatacccaa
     6721 cagagtcgat tttcctccag tgctatactg ccggggaaca agtcctgtgg aagctgcaaa
     6781 atccctgctg catgagaact gtttcccgtc accaagttgc gacgatagta agctggcagt
     6841 aatcggacca acaccaggaa ttgtcataat gcgctgagcg gtatcatcag cgttgattga
     6901 ctgacttaat tctgattcca gctctgctat ttgctcaaca aggtaaaagt agtgggtatg
     6961 caacctcacc aaaaggcggc tcagatattc ggggatttcg tgttcagcca acacgagaga
     7021 caaccgtttt attacagcat cacctatcgg caggctgatt ccaaattcca gcaagaaacc
     7081 gtggatctgg ttggttgttt tcaccttatc tctgatgagc gattctctga cccggtgtaa
     7141 cgccctcatg gcctgctggg tctcagttct tggctggaca aaacgcattg atggtcgtga
     7201 agccgcttcg catatggctt ctgcatcaac aaaatcattc ttattacttt tgacaaacgg
     7261 gcgaacgaac tgtggagaaa tcagcttagt ttcatgccca aaatcagaga tacagcgagc
     7321 cataaagtgt gcacccgcac aggcttccat tacaacaact gagggcggac atgtcgccag
     7381 aaactgcatc agtttagtac gggtaaattt tttacgcaga agagccttac cagacttgtc
     7441 ttgacaatga atgtggaagg agtgctttcc gagatcgata ccaataagtg tgatgttctg
     7501 catgatggtc tctccgttaa agggatttac cttaacagcg tactgctgtt aaggtggggg
     7561 tgaccatatc attatcccca ccggtcccat ctcaccagat tgttgacgat aattttactg
     7621 ccaaatttac aggggcaatt ggttttgtaa ttccagttca gccctctgaa gacgttgatc
     7681 acccctcgct caaacacctc atccttattc tggtgcagtt gttcaaatgt gctcagaatg
     7741 ttcgcttcgc tgatttccgg aaaattatcg tactccagtg accggtacca tgtatcacgg
     7801 gcctgcgtat ccattaatga cagcatcccg gaacgttgca tcaggtcacg ccagatttcg
     7861 cgatcaagat tccgggtaat ggctttcatt gctgtttcgg gtttctccat cagccagcag
     7921 ccgcagcgaa aatcctgttt catggcccag tccggggcag ttttacctcc gatgctttcc
     7981 gtcagcaccg agatttcagc cagttgtcgg ataagtgtgt cgatttgcaa cagtgtactc
     8041 tttcgtacag ccagaatgtg gcgaatgctg gttgagcaga taacgtccgt atgcccgttg
     8101 accacttccg gacgggagat tgtgatatct gacataatcg atattccttt ttgcagataa
     8161 atgaaaacgc cagccctgtg caggctggcg tcagaggaat gaagtctggt gagtgagctt
     8221 caccggttct ggtgcagaag ctgcagatga cgcagggtac ggacatcctc ccgggcctgt
     8281 tcgatacagg gcaacagtgc tgctgaatcc gttttatcct catcgttgtc aaagataatt
     8341 cccgatccgc agtcgatatt gtcctgcagc cacgcaatca gaatatccag cgctgtttcc
     8401 gtggttaatg atttcatgtt gttcctggtt accagtcgaa agtgggtaaa cccggcaggc
     8461 attcagcgct ggcatccaga tgaatgagac tgacgccata acgccggatg agtgtgacga
     8521 ccagacgacg gaacgttttt gacagcccca ggcgtttaag ccgcagaacc gggtatgacc
     8581 acgcatcggt acgtaacaga tatccgctac ctgtaaaatg aatccattct gattcaccga
     8641 agtcactggt ctggtgtgac agcgagtaca accaggcgtt gtccgtttcc gtgatatgtg
     8701 cggtactgca gcgtatgccg gtaagggtag caaatggtgg cgtgcattct gctgaggtct
     8761 ggtcagattc atcaacaaca cgaagggagt agccgttatc gcagaccatg ttaatcagtt
     8821 cagcgagctc aataccatcg acgtcaacga caatgcgccc tatgttcagt gcctgtacgt
     8881 taacgctgtc ggcttccagt gtcagggcca gtttcatcat ttcgcctccg gatacttacc
     8941 cagggtaatg ttatttaccg ttctgtaatt gtcgcgggtc atcaggccgg tcgcccggcg
     9001 agcccggagg atatcaatac tgtttattaa ctgagagcgg ggacaggtgc tgaatcccgg
     9061 ctggtcggta cgcaccagcg cgtatttttc cacgagaaaa ttaactgcat cacacagtga
     9121 aatgcctgcc tcaatatgct gctcaatcac acgttcatcg acaaacggtg tgtcattcag
     9181 tgtgaggcca taatgctggt ccagcagtcg gatgagcagt gtctgccaga tggtgacggg
     9241 agacgggcag cgagatacct cccgtacatg tgtgtcggat aatgttttca tcttaagttc
     9301 tctgtaactg gtagtgctaa aggtgagagc ttcggggtta tgaggtggtt gcgggtgctg
     9361 gtgtcggata aacagccaga taaacgtaac cacaggagcc gagggtgtcg gcttcgcagg
     9421 tcagcccttt tgcatacagc gtgacggtat gctgatatcg gggattcagt tcaccgctgg
     9481 tgagcatgag ttccagttgt ttcatcagca gcggaaaggc ctggtccaga tggtatgcat
     9541 ctgcgtcgct gaagcggcct ctgataccgg cgcggtcggc aaggtaatac aggcggttac
     9601 cctcttgcac cagacgagcg ccaaaacagg gcctcactgt gcagggaagc ccccaccagg
     9661 ggcggtcgtg attgtcatcg ggatgagtta tcccggagag tttgtctgac acgataaaat
     9721 cctctctgaa aagtggttaa gaatggttta acgatgacag gtcagccggc gcgctgaccg
     9781 gtttcggtga agatgtatgc attgatgaga atggcctcat ccacagcggc gtctgaggtc
     9841 atctggtcgt actcgtttcc cgggtgacgg aacatcagtt cagggtgaca ctcatcagcc
     9901 ggacatacgg gccaaaactg tccttacggc gttctgcaaa cacggcaaga acgccgggaa
     9961 tatcctgcac ttcacgacca gtatacgctt cagcactgcc gtgccagcga tacttaccgg
    10021 tacagaacgg aaaaagacgg gatgcaggat gctgttggtg aatacgcatg gcttcaccac
    10081 gggtgatgat tctcatgatg tgatacctct gaagacagaa gataaaagtg aaaacaggtg
    10141 tgatgtggtt gtgacgggtt aaagcagacc atgttcggca aaggagaaaa cctggttgcc
    10201 accgactatc agatggtccg gcacccggat atccaccagg cccagtgcct gtaccagacg
    10261 ttcggtgata agccggtctg ccttactggg tgtgacttca ccggacgggt gattgtgcgc
    10321 cagcaccacg gcagcggcat tgtggtacag ggcgcgttta atcacttccc ggggatgaac
    10381 ttccgtacgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct gattctggtt
    10441 gttcagatac agcacccgga actcttcccg ctccagtccc gccatgttca gaatcagcca
    10501 ttcccgtgcc gcacgggtgg aggtgaaggc cacgccgggt tcatgaagat ggcggtccag
    10561 ggtttgcagg gcccgcagaa tgagactgcg ctcgccgggc gtcatgtttc cgggcagaaa
    10621 ggaaagctgc tgcattgtcc ctgcctccat tcagtcgatg atgcgcataa tggcgctgca
    10681 ttccggatgc tgcagggcgt aatcccgcag ccggtaataa tggaccgtca tggcataaca
    10741 ctccgtacga caggcatgat gactgtacgt catcagacag gcagcaatgc cggcggcttc
    10801 cgggctcatt tcagcgcggt taccgttcat ggcattgaac agtacccatg tttcgtcatc
    10861 atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt acagattcca
    10921 gataccaccg cagtagtctt cacacagacg gtccatccag ccgaagacac ggggttccag
    10981 ggtgacccac tgtggaatga ggccaaaatg ctgcggccag aagctgatgc gctgttcatc
    11041 agggacggga gtggcaacca gttgttgcag gccagtctcc ggtgcacagg ccgaagcggt
    11101 ggtgttctga gacaatgttt tcatgtatga aatccttgta atgtgaataa atgagaggga
    11161 gtaaaggtcg ggagctggca acatcacgtt catcactgac cgtgatgacc cggtaatatc
    11221 agggatgaat gatattcggc tgaaattccg gacagccgta cgcgtaaccg ttccggtcac
    11281 agacggatgg ccagatgcca ttgcctgtat actcagtctg cacagactga tttctccggg
    11341 acggtaaagc agacgggtac accaggcgca caggtcatca tcagaaatgg aacgacaccc
    11401 cggcagcgcc tccggggcgt cgggtgattc tggtgcctga atcacgcctt cagggacatg
    11461 ctgcctcagc gcaggctctc cagcagcgtt tctgccatta cccacaatgc gcggttgagc
    11521 ttaatatcgg tgtcgatgtt gtgaatggca cgggtgtgga tacgttttcc ttttgcactg
    11581 cggccggaaa tcccgccttt cagcatattc tcctggatgg tctgatatgc gctccacagg
    11641 tctttaccgt aatcctccct gcgtcgtggt gtcagaatgt cagcggtggt gactggctga
    11701 tgttcgtcac cataacggta agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc
    11761 ggcaggacca gcgactgcat ggcatcacgc ttctcctcta tccggtcaaa cacgcccacc
    11821 acctcgtaag ccccttcaat aactttctcc accacatttc cccggtgtgg aacacgcaat
    11881 tctcccagag actgaccgca gaccagtcca ttggtacaaa tggcacgaaa atatccgggt
    11941 aacatctggt agctggatga accgtcatga gagttgagca gaataatttc agggacatgc
    12001 tgaccggtta tctgtccggc ccgccgcaga cgcagcatgt gttttgtgta ttcccggcga
    12061 cccgggtcac gcacacgtgt ctggcaggcg aagaatggct gaaagccttc ccgctgcagg
    12121 ctttccagta cggtgatggt gggagtgtac gcatagcgtt tactgcggga agtatgccgg
    12181 tcttcaccga aaatactggg aacatagtgc atcagttctt cgtgtgtcag cggacggtca
    12241 cggcgtatct ggtttgcagc ataaccaaaa cgactggcta gtcgcataat ttgctcctta
    12301 tcggtggtta agatttactg gtgtaataaa tgaaaaagcc acgtctcccg gagaagacgc
    12361 ggcctgatgg acgaaattaa tgaggtttat tgtctgagaa gaccgtcact ggcgaacctt
    12421 ccggcgtcag tctctgcgtt gcggataacc cgacggaaat gagttccctg gtcatcgtcg
    12481 ataaacacat tgcggtaagc tgttgtgatg acaacagcct gctcgcgtga gaaagggact
    12541 tcgggcaata aacgtgacat acaaccagac ctgtatttgg ttccctgaaa atataagtcc
    12601 ctgtcatcat ggcagagccg tatttacttt tgatatcaat aaattaataa cgcgaagatg
    12661 aaccgcataa cttacgcctt taatttgttt tgtttacgta gtccctgacc aggtacgcga
    12721 attcgtgaac gccagtttca ccaaaccaaa tcaggtaacc aaggtcgatg ttgtaatagt
    12781 aaccaacgac tttatgcgtt tttgggttat agagtgttac gatatagtcc cagccgcagg
    12841 cgtgggcttt acaaccgcca ccttctaccc aggtcgttcc gttaatgttt acaggatacg
    12901 caccaccatc aggaccatcc atatagccgt cttccccaaa cattccgttt aacccaacag
    12961 ggtttaatgc tttgtcgata gcagctctgt aggtagcgtc accttcgtat aactcgtacg
    13021 ttgttggaca tgcaccaggc tggtcagccc gggcttttga gtcgcattta agtgtctgat
    13081 ttacagtggc ggcgtttgtg tcaggactt
//
LOCUS       DAZOZX010000107        12908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZOZX010000107 DAZOZX010000000
VERSION     DAZOZX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.107"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUPT_003039"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUPT_003039"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0820509.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACHUPT_003040"
     CDS             complement(1806..3224)
                     /locus_tag="ACHUPT_003040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0820510.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACHUPT_003041"
     CDS             complement(3378..3722)
                     /locus_tag="ACHUPT_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR0820511.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUPT_003042"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUPT_003042"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0820512.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACHUPT_003043"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACHUPT_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUPT_003044"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUPT_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0820513.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUPT_003045"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUPT_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0820514.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUPT_003046"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUPT_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0820515.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACHUPT_003047"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACHUPT_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0820516.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACHUPT_003048"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACHUPT_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0820517.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACHUPT_003049"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACHUPT_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0820518.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUPT_003050"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUPT_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR0820519.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUPT_003051"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUPT_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUPT_003052"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUPT_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR0820520.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUPT_003053"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUPT_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3401 a         3299 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAZOZX010000108        12777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZOZX010000108 DAZOZX010000000
VERSION     DAZOZX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.108"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..222
                     /locus_tag="ACHUPT_003054"
                     /pseudo
     CDS             1..222
                     /locus_tag="ACHUPT_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(719..1095)
                     /gene="rnpB"
                     /locus_tag="ACHUPT_003055"
     ncRNA           complement(719..1095)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUPT_003055"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1128..2273)
                     /gene="garK"
                     /locus_tag="ACHUPT_003056"
     CDS             complement(1128..2273)
                     /gene="garK"
                     /locus_tag="ACHUPT_003056"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0820521.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(2370..3260)
                     /gene="garR"
                     /locus_tag="ACHUPT_003057"
     CDS             complement(2370..3260)
                     /gene="garR"
                     /locus_tag="ACHUPT_003057"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0820522.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(3290..4060)
                     /gene="garL"
                     /locus_tag="ACHUPT_003058"
                     /pseudo
     CDS             complement(3290..4060)
                     /gene="garL"
                     /locus_tag="ACHUPT_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(4076..5410)
                     /gene="garP"
                     /locus_tag="ACHUPT_003059"
     CDS             complement(4076..5410)
                     /gene="garP"
                     /locus_tag="ACHUPT_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0820523.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5785..7356
                     /gene="garD"
                     /locus_tag="ACHUPT_003060"
     CDS             5785..7356
                     /gene="garD"
                     /locus_tag="ACHUPT_003060"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0820524.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(7408..8232)
                     /gene="agaR"
                     /locus_tag="ACHUPT_003061"
     CDS             complement(7408..8232)
                     /gene="agaR"
                     /locus_tag="ACHUPT_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR0820525.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            8481..9761
                     /gene="kbaZ"
                     /locus_tag="ACHUPT_003062"
     CDS             8481..9761
                     /gene="kbaZ"
                     /locus_tag="ACHUPT_003062"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR0820526.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9784..10257
                     /gene="agaV"
                     /locus_tag="ACHUPT_003063"
     CDS             9784..10257
                     /gene="agaV"
                     /locus_tag="ACHUPT_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR0820527.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10268..11047
                     /gene="agaW"
                     /locus_tag="ACHUPT_003064"
     CDS             10268..11047
                     /gene="agaW"
                     /locus_tag="ACHUPT_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR0820528.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11037..11846
                     /gene="agaE"
                     /locus_tag="ACHUPT_003065"
     CDS             11037..11846
                     /gene="agaE"
                     /locus_tag="ACHUPT_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR0820529.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11933..12367
                     /gene="agaF"
                     /locus_tag="ACHUPT_003066"
     CDS             11933..12367
                     /gene="agaF"
                     /locus_tag="ACHUPT_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR0820530.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12364..>12777
                     /locus_tag="ACHUPT_003067"
     CDS             12364..>12777
                     /locus_tag="ACHUPT_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0820531.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         3101 a         3413 c         3218 g         3045 t
ORIGIN      
        1 agaaaatgct tatctggtcg tcccccaaat taattatact atggatttaa acatagaaga
       61 ctctagctca gaagagttaa taccctcgag aaagacgaaa attctccgga caatggcttt
      121 gaggtcgggg aatataacac atatgaagca tataactcag agaatcaata ttttaccaga
      181 gaggactata cgtataatta cgacctttta aatgcaatat agacttaata acttcttaca
      241 aagtctatta ttaataccgt agatattcat cttaccctgt gcaccaaaag caaaaactta
      301 tcgacgcggg tttgaaactg atggcaaccg caaaaatgcc tgatgcgcta cgcttatcag
      361 gcctacgcca tctctgcaat atattgaatt tgcgtgcttt tgtaggcagg ataaggcgtt
      421 cacgcagcat ccggcatgaa taaagcgcac tcaacaatct gaaacccgcc ggaacggttt
      481 attacgtaca tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agtgtcgtgg
      541 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt
      601 atatcaggtg aaactgaccg ataagccgct ttcttttggg tatagcgtcg tggacagtca
      661 ttcatctttc tgcccctcca aaagcaaaaa cccgccgaag cgggttttta cgtaaaacag
      721 gtgaaactga ccgataagcc gggttctgtc gtggacagtc attcatctag gccagcaatc
      781 gctcactggc tcaagcagcc tacccgggtt cagtacgggc cgtaccttat gaacccctat
      841 ttggccttgc tccgggtgga gtttaccgtg ccacggactg ttaccagccg cgcggtgcgc
      901 tcttaccgca ccctttcacc cttacctgat cccgcttgca cgggccatcg gcggtttgct
      961 ctctgttgca ctggtcgtgg gtttcccccc caggcgttac ctggcaccct gccctatgga
     1021 gcccggactt tcctcccctc cacccgtctc ccccgaagag gacgacgacg aagcggcgac
     1081 tgtctggtca gcttcggcgc gcagtataga gggtttgcgc gcccttgtca ccccgtgttg
     1141 cgcattccaa tcgccagtgt cgcggcaata ttacgtgaag cacggcagat attgtcataa
     1201 gccccgcgga atgcttcgtc caacgtacct atgctggtca atacgctgaa gaccgcatca
     1261 atgccatgct gatgtacaac gccaacatca tcggtcaggc tacccgcaat gccaatcacc
     1321 ggtttatggt acttcttcgc cacgtttgcg acgccaatcg gtaccttgcc gtgaatactc
     1381 tggctgtcaa tacgcccttc accggtgatc accagcgtac aatcgtgaat atgttcctcc
     1441 agattcagcg ccgtagtgac gatttcaata ccacttttca gttccgcacc aagaaacgcc
     1501 atcagcgccg cacccatacc gcccgcagct cctgcgccgg ggacatcttt tacatcaaca
     1561 tgcagcgctt ttttaatgac atcggcatag tgagagaggt tattgtccag ctcaacaatc
     1621 atcgcttcac tggctccctt ttgtgggcca aagatgcgcg atgcgccgtt atcgcccacc
     1681 agcggattgg tgacatcaca agcgacgcga atgacgcaat cttttaagcg cggatcgagg
     1741 ccggaaatat caatatcatt cagagtatta agactaccgc cgccaaaacc aatttcattg
     1801 ccgttggcgt cgcataattt cgcccccagc gcctgtacca tgcctgcgcc gccatcattt
     1861 gtagcgctac cgccaatgcc gataataatg tttgtcgcgc cgctctccag cgcctgcagg
     1921 attaactcgc ctgtgccgcg tgaagtggtc acgagtggat cgcgtttttc cgcaggtacc
     1981 agctccagcc cactggccgc cgccatttca ataaacgcgg ttttgccatc gccggagatc
     2041 ccccaactgg cattcacttt ctcgcccagc ggccctgtaa cccaggcgtg acgttcagaa
     2101 ccctgggtgg ctgcaatcat cgcttccacc gttccttcgc caccgtcggc aaccggaata
     2161 gaaacgtact gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc ctgcgcaacc
     2221 tcgctggcag ataaactttc tttataagag tctggggcga ttacgatttt catacctatg
     2281 cctgttacca catgacgccg gagggcgttt ctcttattcg gcctggattc caggcccgga
     2341 ttgcaatacg ccatccgggc acgacgtcat taacgagtaa cttcgacttt cgccagtttt
     2401 tcgtagtagc acaccagggc gctatgatcc gccgttccta aaccatctgc tcgcagtgcc
     2461 tgcatcatct ccataaccgc agctgtgagc ggcagttgcg cgccgacgcc gtgggaagta
     2521 tccagcgcat ttgccagatc cttaatatgc agatcaatac ggaagcccgg cttgaagttg
     2581 cggtccatca ccatcggcgc tttggcatcc agcacggtac tgcccgccag tccaccgcga
     2641 attgcctgat acaccaggtc cgggttaacg cccgctttag ttgccagcgt taacgcttct
     2701 gacatcgcgg caatattcag cgccacaatg acctgattcg ccagtttggt gacgttacct
     2761 gcaccaattg ccccggtatg caccacggaa cccgccattg ctttcatcaa atcatagtat
     2821 ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca gcgtaccgtc gatggctttc
     2881 ggttcaccgc cgctcaccgg agcatccagc atatcaatgc ctttcgcttt cagcgcttcg
     2941 ctgatttcac ggcttgccag cggtgcgata gaactcatat cgatcaatac cgtacctggc
     3001 ttcgcgcctt caataatgcc attctcaccc agcgccacct ctttcacatg aggggagttt
     3061 ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt agaggctgtt
     3121 tctgcacctg cagcaatcac gtcagcaata gcttctgggt tacggtcagc aaccaccagc
     3181 gagtaacctg ctttcagaag gtttttactc attggtttac ccataatccc caggccaata
     3241 aaaccaactt tcatagtcat atcaatcatc tctcttgttg cggtggtggt tattttttaa
     3301 aggtatcagc cagtttatga gtggcagagc ggaagacgcc gagatcgctg ccgacagcca
     3361 caaacgtcgc gccccattcc agataacgac gcgcatcggc ttcgaccggc gcgaggatac
     3421 cgctgggttt gccgtgcgcg ctggcacggt taaaaatgtg ctgaattgct ttttgtacat
     3481 ccgggtgtga tgcattgccg agatggccta atgccgcggc cagatcgctg gggccgacga
     3541 agatgccgtc tacgccttcg gtagcggcaa tggcatcgac gttatctacg ccctgctgac
     3601 tttctatctg gaccagaata gtgatgttct tgttcgactg agcgaaataa tccgccacgg
     3661 tgccaaacat attggcgcgg tgagaaacgg agacgccgcg aatgccttcc ggtgggtaac
     3721 gggttgatgc caccgcctgc tctgcttcct cttttgtttc tacaaaagga atcaggaagt
     3781 tatagaaacc gatatccaga agacgcttaa taatttccgg ctcgttggtc ggcactcaca
     3841 ctactggcgc gctggcgctg cctttcaagg ccattaactg cggaataaac gtggagatat
     3901 cgtttggcgc atgttcgcca tccagcacca gccagtcaaa cccagccaaa ccaagaactt
     3961 cagtgctaat cgggttagag agtgctgacc agcaaccaat ttgtacctgt ttcgcagcca
     4021 gtgcggcttt gaatttattc gggaaaacat cgttattcat cgcttatacc tttgcttatt
     4081 tctgcaattc catacgttta atgtcgccaa ctacgaagag gtagcagacc atcgccatca
     4141 gcgctgaaca tcccacgaaa accagtgctg cattgaagga gtgcagttca cttaccaggt
     4201 agccaatcac cagtggagtg acaatggagg caacattgcc aaagacgtta aagacgccgc
     4261 cacagaggcc aacaatctct ttcggcgcgg tgtcagaaat caccggccag cccagagcac
     4321 caaatccttt gccaaaaaaa gccagcgcca tcagcatgac caccagcgtg gtgttgttgg
     4381 tgtagttaca taagatgatg gtggaagcca gcaacattcc cagcacaatc ggtagcttac
     4441 gtgccagggt cagggataaa ccgcgtttga tcagataatc cgagaagaca cctcccagca
     4501 cgccgcccgc aaaaccacac agtgccggaa tcgaggcgac cagacccact ttcagaatcg
     4561 acatgccttt ttcctgcacc agataaatcg ggaaccaggt gaggaagaac caggtgatgg
     4621 tgttgataaa atattgtccg aaaaatacgc ccaacatcat gcggttagag agcaattgct
     4681 tgatgtaatg cagtttggga ccgcttgctg ccgcgctgcc cggctttttg tggtccatat
     4741 cgaccaccgc gccattttca gagataaact tcagctcttc ctcagacata cgtgggtgat
     4801 ctgtcgggtt atgaatcaac ttgatccaca gcgccgtcag cacaaaacaa atcaccccca
     4861 taacggtaaa gacgtgctcc catccccagg cgaaagtcag ccagccaagc agaggcgaaa
     4921 agagcgccag cgagaaatat tgtgccgagt taaagatggc ggaggcagta ccacgttctt
     4981 tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg gaaggatgga gcttccgaga
     5041 atcccagcat aaagcgcata aagaacatgg agatccctgc ccaagccagc gggaacatat
     5101 caacaaagcc ttgcaggaag gtgaacagcg accagaaaaa gaggctgtag gtgtaaactt
     5161 ttttcgagcc aaacttatca agcaaccagc cgccggggat ttgcatcagc aagtaggccc
     5221 agccaaaagc ggagaagatg taacccatcg aaaccgcact taactgcaac tcttttgcca
     5281 cttcggtacc agcaatagac agcgttgcac gatcggcgta gttaacggcg gtaacaataa
     5341 aaataatcag taatattaaa tagcgggtat gcacaccttt ccttttttcg tcaacggtgt
     5401 ccagaatcat tttatttacc tcgggtactt atgctgattt ttattattat ggggaaggtg
     5461 ttatttataa gtttcattta tgccgtaacg acaatgaact cgggaattag tataagcagc
     5521 gcgagaataa taatcattgt gcaaatgcta atttaattaa tactttttaa atattatttt
     5581 gagcatatgc acataaggtt gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg
     5641 gcactgcaat ccctaaaatg attgacattg atcacatttc tgcgtttaaa ctcctgacat
     5701 tcttatttca cccaatgaaa tcatttattt ttaaatgaga ccaggtcctc attttaataa
     5761 cccctggctg gagaatattg cacaatggcc aacatcgaaa tcagacaaga aacgccaact
     5821 gcgttttata taaaagttca cgacacagat aatgtggcaa ttattgttaa tgataatggc
     5881 ctgaaagcag gaacgcgttt tccggatggg ctggaattaa ttgaacatat tccccagggg
     5941 cataaagtcg cattgctgga cattccggct aatggtgaaa ttattcgtta tggcgaagtg
     6001 attggttacg ccgtgcgtgc aatcccacgc ggaagctgga tcgacgaatc aatggttgta
     6061 ctaccggaag cgccgccgtt acacacgctg ccactggcaa ccaaagtccc ggaaccctta
     6121 ccgccgctgg aaggatacac ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc
     6181 aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg caggcgtggt ggactatgta
     6241 gtaaaaatca ttgaacgcga tctgctaccg aaatacccga acgtcgatgg cgtggtgggg
     6301 ctgaatcatt tgtacggttg tggcgtggcg attaacgcac cggcagcagt tgtacctatt
     6361 cgtaccattc acaatatttc gctgaaccct aactttggcg gcgaagtaat ggtgattggc
     6421 ctgggttgtg aaaagttgca gcctgagcgc ctgctgactg gaacggatga tgtgcaagct
     6481 attccagtag aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt cggttttcag
     6541 tccatggtcg aggatatttt gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt
     6601 cagcgagaaa cctgtccggc ttcagaactg gttgtcggca tgcattgcgg tggcagcgat
     6661 gcgttttctg gcgtaacggc aaacccggcg gttggctatg cgtctgatct actggtgcgc
     6721 tgcggcgcaa cggtgatgtt ttcagaagtc acggaagtgc gtgacgcgat ccatctgctg
     6781 acaccacgcg cagtgaacga agaggtcggc aaacggctgc tggaggagat ggagtggtac
     6841 gataactatc tcaatatggg aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag
     6901 aaaggcggtc tagcaaacgt ggtagagaaa gcgctcggct ccattgctaa atcgggcaaa
     6961 agtgcaattg ttgaagtgct gtcgcccggt caacgcccga ctaaacgcgg attaatttac
     7021 gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc
     7081 gtgcaagtgt ttacgaccgg tcgtggtacg ccgtacggcc tgatggcggt acccgtcatt
     7141 aaaatggcac cccgcaccga gctggcgaac cgctggtttg atttaatgga tattaatgcg
     7201 ggcaccatcg ctaccggcga agaaactatt gaagaggagg gctggaagtt gttccacttt
     7261 attctcgacg tcgccagcgg gaagaagaaa accttctcgg atcaatgggg actgcataac
     7321 cagctggcgg tgtttaaccc agcaccggtg acctgataat tcaattcgta ttatgcccga
     7381 tagcacacgt ttatcgggca tattgagtca cagcgaagac gcgtgatccc cgaccagaat
     7441 cacttcaacc ccagcctttc gcagtccttc cagactatcc gcaggaatgc cttcatcaac
     7501 aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactggaac gattgaattt
     7561 actggaatcg gtgaccacga tgatccgttc cgcaacttcg cacatccgac ggcttaaacg
     7621 ggcttcatct tcattatgtg tgctgacgcc gcgctccaga tcgatcgcat ctacaccaag
     7681 aaacagcata tcgaagtggt aattttgcag cgattgctca gcctgatcgc cgtaaaaaga
     7741 ttgcgactga cggcgcaaat gcccgccggt catcagcagc tcaacgcctt ccgcttccag
     7801 caacgcatta gccacgttca taccgttggt catcgcaatt acgtcagtgt acttgcgcat
     7861 cagacgagca atctcaaaag tggtggtccc ggaatcgagg atcacccgat gacctggctg
     7921 aatcaactca acggcagctt tcgcaacgct gcgtttcatc gcggtgttca gtgcgctttt
     7981 atcttccact gatggctcga ctgacggcgt cgtgctatcg cagatcaacg cgccaccata
     8041 ggcacgcaca gcgatcccct gcttttccag aaacgccaga tcattgcgga tcgtcacagt
     8101 agatacgcca tacaatgccg acagatcgtt aacctgcaca ctcccttgct gtcgcagacg
     8161 ctgaatgatc tgttctcgtc gctcgctggt gcctgtcact cgcttctcac ctgaagcgtc
     8221 ggtattactc atagtaagtc ctttcgtaaa actttcgttt catttcgttt tgcctattaa
     8281 cgcctttcta ttaagcaaat gcaagcccac cttgcccatt ggcgcaagct actctcgttt
     8341 cagtgacttt cattacgttt cttttgtgaa tcagatcaga aaactattat ctttcgtttt
     8401 atttttatct caccatgacg cagtatcaat tgaaacaaaa cgaaagatca atatcgcagt
     8461 aatctgaact ggagaggaaa gtgaaacatc tgacagaaat ggtgagacag cacaaagcgg
     8521 gcaaaacaaa tgcaatttat gccgtttgtt ccgcacatcc gctggtgctg gaggctgcaa
     8581 tccgctacgc cagtgcaaac caaacgccgt tactgattga agcaacctcc aatcaggtag
     8641 accagttcgg cggttatacc ggaatgacgc ccgccgattt tcgcggcttt gtttgtcagc
     8701 tcgccgactc gttgaatttc ccgcaggatg cgctgattct gggtggtgac catctggggc
     8761 caaaccgctg gcaaaacctg ccagccgctc aggcaatggc caatgccgat gatttgatta
     8821 aaagctacgt tgcggcagga ttcaaaaaaa tccaccttga ttgcagcatg tcctgtcagg
     8881 acgatccaat tcccttaact gatgacatcg tggctgaacg cgccgcccgt ctggcgaaag
     8941 tggcggaaga aacctgtcgc gaacactttg gcgaagccga tctggagtat gtcattggta
     9001 ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag cgagctggcg gtcaccacgc
     9061 cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc ctttgaaaag caaggtttga
     9121 atgccatctg gccacgcatt attgccctgg tggttcaacc cggcgtcgaa ttcgatcaca
     9181 ccaacgttat tgattatcag cccgccaaag cgagcgcctt aagccagatg gtcgaaaact
     9241 acgaaacgct gattttcgaa gcgcactcta ccgattatca aacgccgcaa tctctgcgcc
     9301 agctggtgat tgaccacttt gccattctga aagttggccc ggcgctgacc ttcgccctgc
     9361 gtgaagccct tttttctctg gcggcgattg aagaagaact ggtaccagcg aaagcctgtt
     9421 ccggtctgcg tcaggtgctg gaaaacgtga tgctcgaccg cccggaatac tggcaaagcc
     9481 actaccacgg tgacggcaac gcgcgtcgtc tggcgcgtgg ttacagctac tcagatcgcg
     9541 tgcgctatta ctggccggac agccagattg atgacgcttt cgctcatctg gttcgtaatc
     9601 tggcggattc accaattccg ctaccgctca tcagccagta tctgccgctg cagtacgtga
     9661 aagttcgctc cggcgagctg caaccgacgc cacgggaact cattataaac catattcagg
     9721 acatcctggc gcagtaccac acagcctgtg aaggccaata agcaaaacaa agaggaacat
     9781 gctatgccaa atattgtttt aagccggatt gatgaacgct tgattcacgg tcaggtcggc
     9841 gttcagtggg tcggatttgc gggggcaaat ctggtgctgg tagccaacga tgaggttgcc
     9901 gaagatccgg tacaacaaaa cctgatggaa atggtactgg cagaagggat cgccgtacgt
     9961 ttctggacgc tgcaaaaagt tatcgacaac attcatcgcg ccgccgatcg acagaaaatc
    10021 ctgctggttt gtaaaacacc cgccgatttc ctgacgctgg tgaaaggtgg cgttccggtg
    10081 aatcgcatta acgttggcaa tatgcactac gccaatggca aacaacaaat cgccaaaacg
    10141 gtttctgtgg atgcgggcga tatcgcagca tttaacgacc tgaaagccgc tggggtggaa
    10201 tgcttcgttc agggcgtccc gacagagcct gctgtggacc tctttaaatt actttgaggg
    10261 attcatcatg gaaatcagcc tgttgcaggc atttgcgttg ggcattatcg cctttatcgc
    10321 tggcctagat atgtttaacg gcttaaccca tatgcaccgc ccggtggtcc tcggcccgtt
    10381 ggtcgggctg gtacttggcg atctgcatac cggaatttta accggcgata cgctggaact
    10441 agtgtggatg gggctggccc cgctggcggg cgcacagccg cctaacgtga ttatcggtac
    10501 tatcgtcggc acggcgtttg ccattactac tggcgtgaaa cccgatgtcg cggtcggtgt
    10561 cgccgtacct ttcgctgtcg cagtacagat ggggattacc ttcctgttct cggtgatgtc
    10621 tggcgtgatg tctcgctgcg accggatggc ggagaacgcc gatactcgcg gcattgaacg
    10681 cgtgaactat ctggcactgc tggcactcgg cactttctat tttctctgcg ctttcctgcc
    10741 tatctacttc ggcgcggaac atgcaaaaac catcattgat gtcctgccgc aacgattaat
    10801 cgacggcctc ggcgtcgcgg gcggcatcat gccagcaatc ggttttgccg tgctgctgaa
    10861 aatcatgatg aaaaacgtct acatacccta cttcatcctg ggctttgttg ccgcaacctg
    10921 gctcaagtta ccggtgctgg ctattgctgc cgccgcgctg gcgatggcgc tgatcgatct
    10981 gctgcgtaaa tctcctgaac cgactcaacc tgcggcacag aaagaggaat ttgaagatgg
    11041 catctaatca aaccaccctg ccgaacgtca gcgaaaatga agaaacactg ctgactggcg
    11101 tcaatgaaaa cgtgtatgaa gatcggagca ttgccgcgga gctgacgaaa aaagatatca
    11161 accgcgtcgc ctggcgttcc atgctgttac aagcgtcttt taactacgaa cgtatgcagg
    11221 cttctggctg gttgtacggt ctgctgcctg ctctgaaaaa gatccacact aataaacgcg
    11281 acctggcgcg cgccatgaag gggcatatgg gtttcttcaa tacccatccg tttctggtga
    11341 cgtttgttat cggcattatc cttgcgatgg agcgttctaa gcaggacgtt aacagtattc
    11401 agagcaccaa aattgccgtc ggtgcgccgc ttggcgggat tggcgatgcg atgttctggc
    11461 taacgctact gccgatttgt ggcgggatag gcgcaagcct cgcgctacaa ggctctattc
    11521 ttggcgcagt cgtctttatt gtgctgttca acgtggtaca cttggggctg cgttttggtc
    11581 tggcgcatta tgcttaccgc atgggcgtgg cggcgattcc ccttattaaa gcgaatacca
    11641 aaaaagtcgg ccatgctgcg tcaatcgttg gaatgacggt aatcggcgca ctggtggcaa
    11701 cctatgttcg tttaagcacc acgctggaaa tcacggcggg cgacgcagta gttaagttac
    11761 aggctgatgt tatcgacaaa ctgatgcctg ccttcttacc gctggtctac accctgacca
    11821 tgttctggct ggtacgccgc ggctagagtc cgctgcgcct gattgcggtt accgtggttc
    11881 tagggatcgt cggtaaattc tgccatttcc tttaaataca atgaggtatt agatgttaag
    11941 tattattttg acagggcatg gcgggtttgc cagtggcatg gaaaaagcaa tgaagcaaat
    12001 cctcggcgat caatcgcagt ttattgccat cgattttccc gaaacctcaa gcgccgcgct
    12061 gcttacatcg cagcttgaag aggccatcgc ccaactggat tgtgaagacg gcattgtttt
    12121 tctgacggat ttgctgggcg gcacgccgtt tcgcgtagct tcaacgctgg cgatgcaaaa
    12181 accgggctgt gaagtgatta ccggcaccaa tttgcaactg ctactggaga tggtgctgga
    12241 gcgcgaaggg ttaagcggtg aagaattccg tgtacaggcg ctggagtgtg gacaccgagg
    12301 gctgaccagt ctggtggacg agttaggccg ctgccatgaa gaatgtccgg tcgaggaagg
    12361 aatatgacac acgttctgcg cgccagaagg ctgctgactg aagagggatg gttcgatgac
    12421 catcagttgc gtgttgctga cggtgtcatc gcagcaatcg aaccgattcc agtgggcgtg
    12481 actgaacgcg atgcggaacg gctctgcccc gcttacatcg acacccatgt acacggtggt
    12541 gcgggcgttg atgtaatgga tgacgcgccg gatgtactcg acaagctggc aatgcacaag
    12601 gcacgcgaag gtgtcggcag ttggttaccg accaccgtaa ccgcgccgct taataccatt
    12661 catgcggcgc tgaaacgtat tgctcaacgt tgccaacgcg gcggacctgg tgcgcaagtg
    12721 ctggggagtt atctcgaagg accgtacttc acgccgcaga ataaaggcgc gcatccg
//
LOCUS       DAZOZX010000109        12766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZOZX010000109 DAZOZX010000000
VERSION     DAZOZX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.109"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            188..1702
                     /gene="caiT"
                     /locus_tag="ACHUPT_003068"
     CDS             188..1702
                     /gene="caiT"
                     /locus_tag="ACHUPT_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR0820532.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1733..2875
                     /gene="caiA"
                     /locus_tag="ACHUPT_003069"
     CDS             1733..2875
                     /gene="caiA"
                     /locus_tag="ACHUPT_003069"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0820533.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3004..4221
                     /gene="caiB"
                     /locus_tag="ACHUPT_003070"
     CDS             3004..4221
                     /gene="caiB"
                     /locus_tag="ACHUPT_003070"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR0820534.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4295..5848
                     /gene="caiC"
                     /locus_tag="ACHUPT_003071"
     CDS             4295..5848
                     /gene="caiC"
                     /locus_tag="ACHUPT_003071"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0820535.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5957..6742
                     /gene="caiD"
                     /locus_tag="ACHUPT_003072"
     CDS             5957..6742
                     /gene="caiD"
                     /locus_tag="ACHUPT_003072"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0820536.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6748..7338
                     /gene="caiE"
                     /locus_tag="ACHUPT_003073"
     CDS             6748..7338
                     /gene="caiE"
                     /locus_tag="ACHUPT_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0820537.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7424..7819)
                     /gene="caiF"
                     /locus_tag="ACHUPT_003074"
     CDS             complement(7424..7819)
                     /gene="caiF"
                     /locus_tag="ACHUPT_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0820538.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8081..11302)
                     /gene="carB"
                     /locus_tag="ACHUPT_003075"
     CDS             complement(8081..11302)
                     /gene="carB"
                     /locus_tag="ACHUPT_003075"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0820539.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11320..12468)
                     /gene="carA"
                     /locus_tag="ACHUPT_003076"
     CDS             complement(11320..12468)
                     /gene="carA"
                     /locus_tag="ACHUPT_003076"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0820540.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT         3029 a         3308 c         3354 g         3075 t
ORIGIN      
        1 gaaaaacgcc ctctgttcac tcgaaacaaa aatgtgatac caatcacaga atacagctta
       61 ttgaataccc attatgagtt agccattaac gcgtccacga tgttaataat aattatatta
      121 aatgttaaca aaaataaaac aaacgggaac gcaaaataaa tattcgtttt cacagtggaa
      181 ttaactcatg aagaatgaaa agagaaaaac gggaatagaa ccgaaggttt tctttccgcc
      241 attaataatc gtcggcatac tttgttggct tacagtcaga gatctggatg cggcgaatgt
      301 cgttattaat gctgtattca gttacgtcac caatgtatgg ggatgggcat ttgaatggta
      361 tatggtggtg atgcttttcg gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg
      421 tttaggtaac gaaccgccag aatttagcac cgccagttgg atctttatga tgttcgcctc
      481 ctgtacgtct gctgccgtac tgttctgggg atcgattgag atctactact acatctccac
      541 cccgccgttt ggcttagaac cgaactcgac aggagcgaaa gagttggggc tggcttacag
      601 cttgttccac tggggacctc tgccgtgggc cacttacagc ttcctttcag tcgccttcgc
      661 ttacttcttc tttgtccgca aaatggaagt gattcgcccc agctcgacac tggtgccgct
      721 ggcaggtgaa aaacacgcca aagggttgtt cggtaccatc gtcgacaact tctatctcgt
      781 cgccttgatc ttcgcgatgg gtaccagtct gggccttgcc acgccgctgg tgaccgagtg
      841 tatgcaatgg ttgtttggca ttccgcatac cctgcaactg gacgctatca tcattacctg
      901 ctggattatc ctcaacgcca tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc
      961 cagtgacgtg cgtagttacc tgagcttcct gatgctgggt tgggtgttca tcgtcagcgg
     1021 tgccagcttc atcatgaact acttcaccga ttcggtgggg atgttgctga tgtatctgcc
     1081 gcgcatgttg ttctataccg atcccatcgc taaaggcggc ttcccgcagg gctggaccgt
     1141 gttctactgg gcatggtggg tgatttacgc catccagatg agtatcttcc tcgcccgcat
     1201 ctcccgtggt cgtactgtgc gtgaactgtg cttcggcatg gtgatggggc tgacagcatc
     1261 cacctggatc ctgtggaccg tgctcggtag taacactctg ctgttgatgg ataaaaacat
     1321 catcaacatt ccaaatctga tcgaacagta cggtgtggcg cgcgccatca tcgaaacctg
     1381 ggccgctctg ccactcagca ccgccaccat gtggggcttc ttcatcctct gctttattgc
     1441 caccgttacg ctggttaacg cctgctctta taccctggcg atgtctactt gccgcgaagt
     1501 acgcgatggt gaagaaccac cgctgctggt gcgtatcggt tggtcaattc tggttggcat
     1561 tatcggtatt gttctgctgg cgctcggcgg cctgaaaccg attcaaaccg ccattatcgc
     1621 cggaggatgc ccgctgttct tcgtcaacat tatggtgacg ctctccttta ttaaagacgc
     1681 gaaacagaac tggaaagatt aattaacccc caaaatatca agaggttgaa agatggattt
     1741 taatttaaat gatgagcagg aactgtttgt cgccggtatc cgcgaactga tggccagcga
     1801 aaactgggag gcctattttg ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt
     1861 caaagcactg gcggatatgg gtatcgacag tctgctgatc cctgaagagc acggtggtct
     1921 ggacgcgggg tttgttactc tcgccgccgt gtggatggag ctgggacgtc tgggggcacc
     1981 aacctatgta ctgtaccagc tgccgggcgg gttcaatacc ttcctgcgcg aaggcacaca
     2041 agagcagatc gacaagatta tggctttccg cggcaccggt aagcagatgt ggaactcagc
     2101 gattaccgaa ccgggcgcgg gctccgacgt gggtagcctg aaaacgactt atacccgtag
     2161 aaatggtaag atttatctta atggtagtaa gtgttttatt accagtagcg cctacacccc
     2221 gtacatcgtg gtgatggcgc gcgacggggc ttctccggac aaacctgtct acaccgaatg
     2281 gtttgttgat atgagcaaac cgggcatcaa agtgaccaaa cttgagaagc tcggtctgcg
     2341 tatggatagc tgctgtgaaa tcacctttga cgatgtggaa ctggacgaga aagacatgtt
     2401 cggtcgggaa ggtaacggct ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct
     2461 ggtagccctg accaactacg gtacggcgat gtgcgccttt gaagatgctg cgcgctacgc
     2521 caaccagcgc gtgcagtttg gcgaggctat tggtcgtttc cagttgattc aggaaaaatt
     2581 cgcccacatg gcgatcaaat taaactccat gaaaaacatg ctgtatgaag cagcgtggaa
     2641 agcagacaac ggcaccatca cctctggcga tgcagcgatg tgcaaatact tctgcgccaa
     2701 tgcggcattt gaagtggtgg atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg
     2761 caaccaccgc atcagccgct tctggcgtga cctgcgtgta gaccgcgttt ccggaggatc
     2821 tgacgaaatg cagatcctga cgctgggtcg tgcggtgctg aagcaatacc gctaagtccg
     2881 tgcgctgcct gatgcgacgc tgacgcgtct tatcaggcct acgggagcac gaatgtaagc
     2941 cggataaggc gtttacgccg catccggcag tcatgcgcta cataaatttt tcaggagcta
     3001 ataatggatc atctacccat gccgaaattc ggaccgctgg ccggattgcg cgttgtcttc
     3061 tccggtatcg aaatcgccgg accgtttgcc ggacaaatgt tcgcagaatg gggcgcggaa
     3121 gttatttgga tcgagaacgt cgcctgggcc gacaccattc gcgttcaacc gaactacccg
     3181 cagctctccc gccgcaattt gcacgcgctg tcgttaaata ttttcaaaga tgaaggccgc
     3241 gaagcgtttc tgaaattaat ggaaaccacc gatatcttca tcgaagccag taaaggtccg
     3301 gcctttgccc gtcgtggcat taccgatgaa gtactgtggc agcacaaccc gaaactggtt
     3361 atcgctcacc tgtccggttt tggtcagtac ggcaccgagg agtacaccaa tcttccggcc
     3421 tataacacca tcgcccaggc cttcagtggt tacctgattc agaacggtga tgttgaccag
     3481 ccaatgcctg ccttcccgta taccgccgat tacttttctg gcctgaccgc caccacggcg
     3541 gcgctggcag cactgcataa agtgcgtgaa accggtaaag gcgaaagtat cgacatcgcc
     3601 atgtatgaag tgatgctgcg tatgggccag tacttcatga tggattactt caacggcggc
     3661 gaaatgtgcc cgcgcatgag caaaggtaaa gatccctact acgccggttg cggtctgtat
     3721 aaatgtgccg acggctacat cgtgatggaa ctggtgggca ttacccaaat tgaagagtgc
     3781 tttaaagata ttggcctcgc acatctactc ggtacgccgg aaatcccgga aggcactcag
     3841 cttatccacc gtatcgaatg cccttacggc ccactggtag aagaaaaact cgatgcctgg
     3901 ctggcggcac ataccatcgc agaagttaaa gaacgctttg ccgaactgaa tatcgcctgt
     3961 gccaaagtac tgaccgtacc ggaactggaa agcaatccac agtatgttgc ccgtgaatca
     4021 atcactcagt ggcaaacgat ggatggtcgc acctgcaaag ggccgaacat catgccgaaa
     4081 ttcaaaaata accccggaca aatctggcgc ggaatgccct cacatggcat ggacacggct
     4141 gccattttga aaaatatcgg ctacagcgaa aacgacattc aggagttggt cagcaaaggt
     4201 ctggccaaag ttgaggacta aacgcgtggc gtcagtcggc ttgcctgact agcgcccgcc
     4261 caataacaaa cgaacaaaaa tggatagagg tgcaatggat atcattggcg gacaacatct
     4321 acgtcaaatg tgggacgatc tggcggacgt ttacggtcat aaaacggcgc tgatttgtga
     4381 atccagcggc ggagtcgtta accggtatag ttatcttgag ttaaatcagg agattaaccg
     4441 cacggcaaac ctgttttata cgctggggat tcgcaaaggc aacaaggttg cactacatct
     4501 cgacaactgc ccggaattta tcttttgctg gttcgggctg gcaaaaattg gcgcgattat
     4561 ggtgccgatt aacgcccgcc tgttacgcga ggaaagcgag tggatcttgc aaaatagcca
     4621 ggcgtgcctg ctggtgacca gtgcgcaatt ttatcctatg tatcaacaga ttcagcagga
     4681 agatgccact caattgcggc acatttgcct gacagatgtg gcacttcccg ctgatgatgg
     4741 agtgagttca tttactcaac tgaaaaatca acaacctgcc accttgtgct atgcaccgcc
     4801 gctatcgact gacgatacgg cggaaattct cttcacctcc ggcaccacct cccgaccgaa
     4861 aggtgtggtg attacccatt acaacctgcg cttcgctgga tattactccg cctggcagtg
     4921 tgcactgcgt gacgatgacg tctacatgac ggtaatgcct gcgtttcata tcgattgcca
     4981 gtgtactgcg gcgatggcgg cgttttctgc cggggccacc tttgtgctgg tcgagaaata
     5041 cagcgcccgc gccttctggg gacaggcgca gaagtaccgc gccaccatta ccgaatgtat
     5101 tccgatgatg atccgtacgt tgatggtgca gccgccttca gcgaacgatc ggcaacaccg
     5161 cctgcgggaa gtgatgtttt atctcaactt gtcggagcag gaaaaagacg cattttgtga
     5221 acgcttcggc gttcgcttgc tgacgtctta tgggatgacg gaaaccattg tgggcattat
     5281 cggcgatcgc cctagcgata aacgacgctg gccgtcgatt ggtcgggcgg ggttttgcta
     5341 cgaagcggag atccgcgacg atcacaatcg cccgctcccg gcaggtgaga tcggtgaaat
     5401 ctgtattaaa ggcgtaccag ggaaaaccat cttcaaagag tattttctca acccgaaagc
     5461 cactgcgaaa gtgctggaag ccgacggctg gctacatacc ggcgataccg gataccgcga
     5521 cgaagaaggc tttttttatt tcgtcgatcg ccgctgcaat atgatcaaac gcggtggcga
     5581 gaatgtctcc tgcgtggagc tggaaaatat catcgccacc catccgaaaa ttcaggacat
     5641 cgtggttgtg ggtattaaag attcgattcg cgatgaagcc atcaaagcat ttgtggtgct
     5701 gaatgaaggt gaaacattga gcgaagagga attttttcgc ttctgcgaac aaaatatggc
     5761 gaaatttaaa gtgccctctt atctggagat cagaaaagat ctgccacgta attgctcggg
     5821 gaaaataatt agaaagaatc tgaaataaat gaaacgtcag ggaactaccc tgccagctaa
     5881 taatcacgcc ctaaaacaat atgcattttt tgcagggatg ttgtcgtccc taaaaaagca
     5941 aaaatggaga aaaggaatga gtgaatcatt acatctgacc cgcaatggat caattctgga
     6001 aattaccctt gatcgcccaa aagcgaatgc tattgatgca aaaaccagct ttgaaatggg
     6061 cgaagtattt ctaaatttcc gtgacgatcc gcaattacgt gtcgccatta ttaccggtgc
     6121 cggagagaaa ttcttttccg cgggctggga tttaaaagcg gcagcagaag gcgaagcacc
     6181 ggatgctgac tttggcccgg gtggttttgc gggattaacc gaaattttca atctcgacaa
     6241 accagttatc gcagctgtga acggctacgc ctttggcggc ggtttcgaac tggcgctggc
     6301 ggcagatttt attgtttgtg ccgataacgc cagcttcgcc ctgccggaag ccaaactggg
     6361 catcgttcct gacagcggcg gtgtgctgcg tctgccgaag atcctgccgc ctaccatcgt
     6421 caatgaaatg gtgatgaccg gcagacgaat gggcgcagaa gaggcgctgc gttgggggat
     6481 agtcaaccgc gtggttagcc aggcggaact gatggataac gcccgcgaac tggctcagca
     6541 gctggttaac agcgccccgc tggcgattgc ggcgctgaaa gagatctacc gcaccaccag
     6601 cgaaatgccg gtagaagaag cgtatcgcta tattcgcagc ggcgtgttga aacactatcc
     6661 atcggttctg cattcggaag atgccattga agggccgctg gcgtttgccg agaagcgcga
     6721 tccggtgtgg aaaggacgtt aacaaccgtg agctattacg cctttgaggg gttaattccg
     6781 gtggttcacc cgacggcgtt tgtccatccc agtgccgtct tgattggcga tgtgattgtg
     6841 ggagccggtg tctacatcgg cccactcgcc tcactgcgtg gtgactacgg gcggttgatc
     6901 gtgcaagcgg gagccaatat tcaggatggc tgcattatgc atggctactg cgacactgac
     6961 accatcgttg gggaaaacgg ccatatcggg catggagcga tcctgcatgg ttgtgtgatt
     7021 ggtcgcgatg cattggtcgg gatgaacagc gtaattatgg atggcgcggt cattggcgaa
     7081 gagagcattg ttgccgcaat gagctttgtc aaagcaggct ttagtggcga gaaacgccag
     7141 ttgttgatgg gtacgccagc ccgcgctgta cgcagtgtta gtgacgacga gttacactgg
     7201 aaacggttga ataccaaaga gtatcaggat cttgtcgggc gctgtcatgc atcgttacat
     7261 gaaacgcagc cactgaggca aatggaggaa aatcgccccc gtttgcaggg gacgacggat
     7321 gtgacgccta aacggtgatc ttgccggatg cgacctggtt gctggataag atgctacaac
     7381 atcgtatctg gcaagcagga cgcccggtat caacgagata aaattaacga cgcatactct
     7441 ttgacaacat catctgccac ttctgctcgc ggttaagttc cgtgagcgga acggcaggaa
     7501 tacgcggtgt ctttcttacg ccgactaatt tttcccgact gttattgcgc aggcgcgcgt
     7561 aaatttgctc atcgatatcg accactttaa ccagtcgctg acactggcat ccccgccctt
     7621 ccagcttatt agggatcatc ttaacttcgc agcttatttc tgtgacttcc gacagaatat
     7681 aagtcagggt attaaccgcc ttgctgtgtt caatatcgaa atgaatagag atctctcttg
     7741 ctatcaccca ccgattttca gccatcatcc attcggcgat taacaagtag agtggttttt
     7801 caacatatcc ttcacacata cgaactccag tttcacaaat taaaagtcta gctatgactc
     7861 gttttgaagc aattaaacaa ggccatatta aaattgtgga tatatcgata gtatgcaact
     7921 tcaccgtcga taagtgtgag ctaaatcctg cttatgtcat caaaaatatc gattccccaa
     7981 aagatttact taatgggcaa aaaaaaacgg tcctcatcag agaaccgtat tagttattcg
     8041 atcaaattag cggatgaaaa atatccgcca cgacacgcta ttatttgatc tgtgcgtgca
     8101 tttcctgcac cgaaattact ttttcagtcg catcggcatt cagcgccatc gcggtagcga
     8161 aaccaccgtt cagggtggtg tcataatgca ctttatattg cagcgcactg cgacggatca
     8221 cgcgggagtc ttcaatcgca cgacggcctg aggtggtgtt gatgatgtag gtatattcgc
     8281 cattcttgat acggtcctga atgtgcggac ggccttcatg caccttgttt accagacgcg
     8341 gattgatacc cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc
     8401 cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg
     8461 aaagcagcgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc gctttggcga
     8521 acgcttcagc gaaggtgcgg cccacgccca tgacttcccc ggtagagcgc atttctggcc
     8581 ctaacagcgg gtcaacgcct gggaatttat tgaacggcag caccacttct ttcaccgagt
     8641 agtacggcgg aataacttct ttggttacgc cctgctcagc cagcgatttg ccagccatca
     8701 cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac ggaacagtac
     8761 gcgccgcacg cgggtttact tcaatcaggt aaacttcgtt gtttttcacc gcaaactgca
     8821 cgttcatcag accgcgcacc tgcaattcga aggccagttt ctgcacctgc tggcgcatca
     8881 catcctgaat ttcctgactt aaggtgtacg ctggcagcga acatgcggag tcaccggagt
     8941 gcacacccgc ctgctcgatg tgctccatga tgccgccaat cagcaccatt tcgccgtcgc
     9001 agatggcatc cacgtcaact tctactgcgt catcaaggaa atgatccagc aacactggcg
     9061 catcgttaga cacgctaacc gccgtctgga agtagcgacg caggtcagct tcgtcataga
     9121 cgatttccat cgcccgaccg ccgagaacgt aagacggacg caccaccagc gggtagccaa
     9181 tctcttttgc cttctcaact gccatttcaa tagcagtaac ggtggcgttc gccggttgtt
     9241 tcagtttcag acggtcaacc gcatgctgga agcgttcacg gtcttccgca cggtcgatag
     9301 catccgggct ggtgccgata accggtacgc cagcggcttc cagtgcacgc gccagtttca
     9361 gcggggtctg accgccgtac tgaacgataa cgcctttcgg cttctcaata cgcacgattt
     9421 ccagcacatc ttccagagtt accggctcga agtagaggcg atcggaagtg tcgtagtcgg
     9481 tagagacggt ttccgggtta cagttaacca taatggtttc gtaaccgtct tcacgcagcg
     9541 ccagcgaggc gtgtacgcag cagtagtcga attcgatacc ctgaccgata cggtttggac
     9601 cgccgccaag caccatgatt ttttcacggt cggtagacgg attcgcttcg cactcttctt
     9661 cataagtgga gtacatgtaa gcggtgtcgg tggcaaactc tgccgcacag gtatccacgc
     9721 gcttgtagac cgggtgcagg tcatattggt cacgcagctt acggatttcc gcttcgcgta
     9781 cgcccgccag ttttgccaag cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga
     9841 attcagcgtt caggccagtg atgcccactt ccgccacttt ctcttccaga cgcaccagtt
     9901 cttcaatctg taccaggaac cagcggtcaa tgttagtcag gttgaagacg ccgtccacag
     9961 acaggcccgc gcggaaagca tcggcgatgt accagatacg ctctgcgcct gcgtctttca
    10021 gttcgcgacg gattttggtt aacgcttccg ggtcatccag gctcactttc gggtcgaagc
    10081 cagttgcgcc cacttccagg ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac
    10141 caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg ttagcaccgg
    10201 cgaatttttc gaagttgaag cgaggaattt tagtaaccac atagtcgatg gacggctcga
    10261 aggaggccgg agtacgtccg ccagtgatgt cgttcatcag ttcgtctagg gtgtaaccca
    10321 ccgccagttt cgccgccact ttagcaatcg ggaaaccggt cgctttcgac gccagcgccg
    10381 aagaacggga cacgcgtggg ttcatttcga taacaatcag gcgaccgttt ttcgggttca
    10441 ccgcaaactg gacattggaa ccaccggttt caacgccgat ttcacgcagc accgccatcg
    10501 aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc gcgacagtga
    10561 tggagtcacc ggtgtggatg cccatcgcat caaagttttc gatagagcag acgatgatgc
    10621 agttgtcgtt tttatcacgc accacttcca tctcgtactc tttccagccg atcagcgact
    10681 catcaatcag caactctttg gtcggagaga gatccagacc gcgggcgcaa atttcttcaa
    10741 actcttcacg gttataagcg ataccgccgc cgctaccgcc catggtaaag gatgggcgaa
    10801 taatgcacgg gaagcccacg tcagcggcaa cagccagcgc ttcttccatc gtgtgtgcga
    10861 taccggaacg cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt
    10921 cttctgcttt atcaatcgca tcggcagtgg caccaatcat ggtgacaccg aactcttcca
    10981 acacgccctg acgttccagc tccagcgcgc agttcagcgc cgtctgaccg cccatggttg
    11041 gcagcaccgc gtccgggcgc tctttttcaa taatcttgcg taccacttcc cagtgaatcg
    11101 gctcgatgta ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc gggttggagt
    11161 tcaccagaat gacgcggtaa ccctcttcgc gcagggcttt acatgcttgc gcgccagagt
    11221 agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc aggatacttt
    11281 ttatatctgt acgttttggc atggctcttt tactcctgat tacttagcgg ttttacggta
    11341 ctgctcaatt aacttgataa agtggtcgaa caacggcgcg gcgtcgtgtg gaccagggct
    11401 ggcttcaggg tgaccctgga agctgaacgc cggcttatcg atgcgatgaa tgccctgtaa
    11461 cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt gcaggtaatg ttgcttcatc
    11521 caccgcaaaa ccgtggttct gggcggtgat catcaccacg tttttctcca catctttaac
    11581 cggatggttg ccgccgtggt gaccaaattt cattttgaca gtcttcgcac cgctcgccag
    11641 cgccagcagc tgatgaccga gacagatgcc gaataccgga atatcggttt cgaggaactt
    11701 ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa
    11761 gatgccgtct ggattcattt tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt
    11821 caggcgacag cctctatcca ccagcatccg caggatgttg cgcttggcac caaaatcata
    11881 agccacgacg tggaacggca gctcgtcttc ttttttcgct tctggcaggc cgccggtcaa
    11941 cgtccagctc ccttgtgtcc agctataggc ttctgcggtg gtcacttctt ttgccagatc
    12001 catgccgttc agacctggga acgcgcgggc tttttctaac gccagcgccg catccgggtt
    12061 atcgcctgcg ataatgcagc cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt
    12121 acgggtatcg atatcggcaa tcgccacgat gttatggcgc ttcaggtaag aagagaggtc
    12181 ttcggtatta cggaagttgc tggcaatcag cggcaggtcg cgaatcacca gaccttgtgc
    12241 atgtacctga gaagattctt catcggcgtc attagtgccg acattgccaa tatggggata
    12301 agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt gataaccggt
    12361 cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta tggcccgacc
    12421 gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa caccctccag
    12481 agaatactca ctcactttat ttgcatatta attcacagtg atgacgtaaa tcaatgcaaa
    12541 tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt
    12601 caacttaatg gcaggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta
    12661 agcgacaaaa acatcatcta acctgttctg gaaaacgctt gcgcagcaag aagattgcat
    12721 gaaataaaaa cgagcaggaa aaagtggacc aaatggtcaa aattta
//
LOCUS       DAZOZX010000110        12389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZOZX010000110 DAZOZX010000000
VERSION     DAZOZX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.110"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="ACHUPT_003077"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="ACHUPT_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0820541.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="ACHUPT_003078"
     CDS             complement(2005..2928)
                     /locus_tag="ACHUPT_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0820542.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="ACHUPT_003079"
     CDS             3145..4488
                     /locus_tag="ACHUPT_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0820543.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="ACHUPT_003080"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="ACHUPT_003080"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0820544.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="ACHUPT_003081"
     CDS             6508..6732
                     /locus_tag="ACHUPT_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0820545.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="ACHUPT_003082"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="ACHUPT_003082"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0820546.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHUPT_003083"
     CDS             complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHUPT_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0820547.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="ACHUPT_003084"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="ACHUPT_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0820548.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="ACHUPT_003085"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="ACHUPT_003085"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0820549.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLTRKK"
     gene            10315..10971
                     /locus_tag="ACHUPT_003086"
     CDS             10315..10971
                     /locus_tag="ACHUPT_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR0820550.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHUPT_003087"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHUPT_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12389
                     /locus_tag="ACHUPT_003088"
                     /pseudo
     CDS             11503..12389
                     /locus_tag="ACHUPT_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3231 a         2903 c         3109 g         3146 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctgaca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca taaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaaga cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac
    12181 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    12241 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
    12301 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
    12361 cgcaatgtcc gggcaaaatc aggtttaaa
//
LOCUS       DAZOZX010000111        12334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZOZX010000111 DAZOZX010000000
VERSION     DAZOZX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.111"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHUPT_003089"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHUPT_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0820551.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACHUPT_003090"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACHUPT_003090"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0820552.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACHUPT_003091"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACHUPT_003091"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0820553.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACHUPT_003092"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACHUPT_003092"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0820554.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACHUPT_003093"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACHUPT_003093"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0820555.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACHUPT_003094"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACHUPT_003094"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0820556.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACHUPT_003095"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACHUPT_003095"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0820557.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACHUPT_003096"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACHUPT_003096"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0820558.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHUPT_003097"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHUPT_003097"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0820559.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHUPT_003098"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHUPT_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0820560.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHUPT_003099"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHUPT_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHUPT_003100"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHUPT_003100"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0820561.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..>12334)
                     /locus_tag="ACHUPT_003101"
     CDS             complement(11944..>12334)
                     /locus_tag="ACHUPT_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820562.1"
                     /translation="FEFKYFYSEYLNFNSRLESRYSYELMKKASEYSELYGDNLIQLG
                     LEDGIYFYKGMAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRS
                     YANFNPEKYPKFKVLMDTPNGILIAIK"
BASE COUNT         3118 a         3125 c         3080 g         3011 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaa
//
LOCUS       DAZOZX010000112        12299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZOZX010000112 DAZOZX010000000
VERSION     DAZOZX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.112"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHUPT_003102"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHUPT_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0820563.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUPT_003103"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUPT_003103"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0820564.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUPT_003104"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUPT_003104"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0820565.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUPT_003105"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUPT_003105"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0820566.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUPT_003106"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUPT_003106"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0820567.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUPT_003107"
                     /pseudo
     CDS             complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUPT_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7849..7902
                     /locus_tag="ACHUPT_003108"
     CDS             7849..7902
                     /locus_tag="ACHUPT_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820568.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUPT_003109"
     CDS             complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUPT_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0820569.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUPT_003110"
     CDS             8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUPT_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0820570.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUPT_003111"
     CDS             complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUPT_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0820571.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10966..12165
                     /locus_tag="ACHUPT_003112"
     CDS             10966..12165
                     /locus_tag="ACHUPT_003112"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0820572.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3004 a         3123 c         3302 g         2870 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgccagcaag aacgcggcag cctgcgtcag
     6421 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     6481 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     6541 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     6601 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     6661 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     6721 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     6781 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     6841 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     6901 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     6961 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     7021 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     7081 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     7141 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     7201 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     7261 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     7321 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     7381 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     7441 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     7501 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     7561 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     7621 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     7681 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     7741 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
     7801 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
     7861 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
     7921 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
     7981 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
     8041 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
     8101 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
     8161 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
     8221 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
     8281 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
     8341 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
     8401 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
     8461 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
     8521 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
     8581 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
     8641 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
     8701 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
     8761 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
     8821 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
     8881 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
     8941 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
     9001 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
     9061 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
     9121 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
     9181 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
     9241 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
     9301 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
     9361 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
     9421 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
     9481 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
     9541 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
     9601 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
     9661 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
     9721 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
     9781 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
     9841 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
     9901 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
     9961 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    10021 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    10081 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    10141 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    10201 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    10261 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    10321 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    10381 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    10441 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    10501 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    10561 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    10621 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    10681 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    10741 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    10801 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10861 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10921 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10981 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    11041 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    11101 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    11161 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    11221 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    11281 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    11341 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11401 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11461 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11521 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    11581 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    11641 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    11701 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    11761 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    11821 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    11881 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    11941 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    12001 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    12061 ggtgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    12121 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    12181 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    12241 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttat
//
LOCUS       DAZOZX010000113        12286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZOZX010000113 DAZOZX010000000
VERSION     DAZOZX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.113"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACHUPT_003113"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACHUPT_003113"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0820573.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..3049
                     /locus_tag="ACHUPT_003114"
     CDS             1649..3049
                     /locus_tag="ACHUPT_003114"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0820574.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            3205..4740
                     /gene="gadC"
                     /locus_tag="ACHUPT_003115"
     CDS             3205..4740
                     /gene="gadC"
                     /locus_tag="ACHUPT_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0820575.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            4871..6190
                     /locus_tag="ACHUPT_003116"
     CDS             4871..6190
                     /locus_tag="ACHUPT_003116"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0820576.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            6567..7637
                     /gene="dosC"
                     /locus_tag="ACHUPT_003117"
                     /pseudo
     CDS             6567..7637
                     /gene="dosC"
                     /locus_tag="ACHUPT_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            7637..8254
                     /locus_tag="ACHUPT_003118"
                     /pseudo
     CDS             7637..8254
                     /locus_tag="ACHUPT_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            8512..8844
                     /gene="ddpX"
                     /locus_tag="ACHUPT_003119"
                     /pseudo
     CDS             8512..8844
                     /gene="ddpX"
                     /locus_tag="ACHUPT_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            8858..9754
                     /gene="ddpC"
                     /locus_tag="ACHUPT_003120"
     CDS             8858..9754
                     /gene="ddpC"
                     /locus_tag="ACHUPT_003120"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0820577.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            9751..10725
                     /locus_tag="ACHUPT_003121"
     CDS             9751..10725
                     /locus_tag="ACHUPT_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0820578.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            10718..11644
                     /locus_tag="ACHUPT_003122"
                     /pseudo
     CDS             10718..11644
                     /locus_tag="ACHUPT_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(11700..12131)
                     /gene="osmC"
                     /locus_tag="ACHUPT_003123"
     CDS             complement(11700..12131)
                     /gene="osmC"
                     /locus_tag="ACHUPT_003123"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0820579.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3033 a         2961 c         3120 g         3172 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc
     1981 tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat
     2041 gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc
     2101 aacggataaa ccaaacctag tgtgcggtcc ggtacaaatc tgctggcata aattcgcccg
     2161 ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt ttatggaccc
     2221 gaaacgcatg attgaagcct gtgacgaaaa caccatcggc gtggtgccga ctttcggcgt
     2281 gacctacacc ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca
     2341 ggccgacacc ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc
     2401 accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag
     2461 tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga
     2521 cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg
     2581 tacttttgcc atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt
     2641 cctgcgcctc ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc
     2701 ttatctggcg gatgaaatcg ccaaacaggg gccgtatgag ttcatctgta cgggtcgccc
     2761 ggacgaaggc atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac
     2821 cctgtacgac ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct
     2881 cggcggtgaa gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat
     2941 ggactttgct gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca
     3001 cccgaaactg cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac
     3061 ggtaacggtg tcccgaaacg aacccgtttc gggataattt ccaaagtctg ttcactggca
     3121 ttagcaacgg aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca
     3181 ggaataccgt tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag
     3241 ctcacattac tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac
     3301 cctaccttcg caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta
     3361 tggtttattc ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa
     3421 ggtggtgtct tcgcctgggt atcaaatact ctgggtccga gatggggatt tgcagcgatc
     3481 tcatttggct atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg
     3541 gcactctcct acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt
     3601 gcagcactca tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg
     3661 gcgcgaattg ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc
     3721 gcattagcgg ctatttacct gcactccggt gcccccgttg ctatcgaaat ggattcgaag
     3781 accttcttcc ctgacttctc taaagtgggc acactggttg tatttgttgc cttcattttg
     3841 agttatatgg gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc
     3901 gactatccac tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc
     3961 ggtttgtcta ttgcgatggt cattccgggt aatgaaatca acctctccgc aggggtaatg
     4021 caaaccttta ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg
     4081 atctccgcac tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct
     4141 tctcgcggga tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg
     4201 aacaaaaatg gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg
     4261 ttgatcatcc tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg
     4321 ctgacggtgg tgatttatct gtgtgcttat ttcatgctgt ttattggcta cattgtgttg
     4381 gttcttaaac atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa
     4441 ctggtcgtgg caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg
     4501 ccgccggata acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt
     4561 ttcctggtgg tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa
     4621 gcgaataccg gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc
     4681 ttcctgcacc cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa
     4741 ccatgtctga atggttataa gacaagggag cgataattca tcgctccctt tttcgtgctt
     4801 gctgtcgtat tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa
     4861 accagcacat atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat
     4921 actggttgca ttggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt
     4981 gacaccacca gcaggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg
     5041 tggcatctgg ctggccacgg tttctcgcct cgactggcca ccggtttcct cggttaacat
     5101 tagtaatccc accagccgag cccgtgtaca acaacaggcg atgatcgaca aactggatca
     5161 tctccaacgt ctcggcataa acacggtctt tttccaggtc aagccggacg gtaccgccct
     5221 gtggccatcg aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc
     5281 gggttacgat ccgctgcaat tcatgctcga cgaagcccac aagcgtggga tgaaagtaca
     5341 cgcctggttt aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact
     5401 gaatagcact ctgtctcaac aaccggcgag cgtctatgtg caacaccgtg actggatcag
     5461 aacgtctggc gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac
     5521 atcaatagtc gctgaagtgg tttcccgcta tccggtagat ggcgtgcagt ttgacgacta
     5581 tttctatact gaatcaccgg gttcacggct aaatgataac gaaacgtacc gtaaatacgg
     5641 aggcgcattt gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa
     5701 ggtatcacac accattaaaa gcattaagcc gggagtcgaa tttggcgtta gcccggcagg
     5761 cgtgtggcgt aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta
     5821 tgacgaatcc tacgcagaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc
     5881 tccccaaatt tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg
     5941 gtgggcggat gtcgttaaac caaccaggac ccgcctgtat atcggtatcg ccttctataa
     6001 agtgggtgaa ccttcaaaga tagagccaga ctggatgatt aacggcggcg taccggaact
     6061 gaaaaagcag ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg
     6121 tgaggactat ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg
     6181 gggcagttaa actaccatgc ttactttgta acaagccggt gctttaccca ccggcttgtt
     6241 acaccttgtg aaatatccca ctagtaatca tgcttacata agtcaaatta accacgcaat
     6301 taaccacact tagttaaaca gctataaatc cctaataaaa atgtatgaat atgaaaaacc
     6361 atacgatatt cgctgtcacc gaaaaataat tacgatgggc tatactcttc tgaagaacag
     6421 tagcgtagat tgaaactgtc ttaagtcgct tatttctttt cagtatatct tcatatttca
     6481 ggagaatatg gtgcagttag tcgatctcgc acgttgcgtt tcgttttgat tccgtctgat
     6541 ttcccagcct tataagggtg tgaattatgg agatgtattt taaaagaatg aaagatgagt
     6601 ggaccggact tgtcgaacag gcagatcctc tcattcgtgc taaagccgca gaaattgccc
     6661 ttgcgcatgc tcattatctg agtatcgagt tttatcgaat tgtccgcatc gacccgcatg
     6721 ccgaagaatt cttgagtaat gaacaagttg agcggcagtt gaaaagtgcg atggaacgct
     6781 ggattattaa cgtgctttct gcccaggttg acgatgtcga aaggctaata caaatccagc
     6841 ataccgtcgc ggaagtgcat gcccgcatag gaattccggt agaaattgtc gagatggggt
     6901 ttcgggtgct gaaaaaaatc ctctatccgg taatcttctc ttcggattat tccgccgcag
     6961 aaaaacttca ggtctaccat ttctcgatta acagtattga tatcgcgatg gaagtgatga
     7021 cccgcgcgtt tacctttagt gacagtagtg cctcaaagga agatgaaaac tatcgtatct
     7081 tctcgttact ggaaaacgcc gaagaagaaa aagaacggca aatagcctca atactttcat
     7141 gggaaataga tattatctat aaagtcctgc tggattctga tttaggcagc agtttgcctt
     7201 taagccaggc tgattttggc ctgtggttta accataaagg tcgacattat tttagcggca
     7261 ttgccgaagt gggccatatc tcccgtctga ttcaggattt cgacggtatt ttcaatcaaa
     7321 ccatgcgtaa caccaggatt ttgaataaca gaagtttgcg ggtgaaattt ctattacaga
     7381 taagaaatac cgtatcgcag attattacct tgctgcgtga attgtttgaa gaagtatcgc
     7441 gccacgaagt cggtatggat gtactgacga aattacttaa ccgccgtttc ctgccgacta
     7501 tcttcaaacg cgaaattgcc catgccaacc ggaccggtac accgctgtca gtgctgatta
     7561 ttgacgttga taaattcaaa gagatcaacg atacgtgggg ccataacact ggcgatgaaa
     7621 ttctgcgtaa agtctcattc ctctcgcaga agagattggt gaaatcgaaa atattgggcg
     7681 ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg cgtagccaga atattcatat
     7741 cccggcgtta tccgtgaact tgtcggcgct gcactttcgc agtaatcagc tgcctaatca
     7801 ggtgtctgat gcaatgcaag cctggggtat tgacggccac cagctgacgg tggaaatcac
     7861 ggaaagcatg atgatggaac acgataccga aatctttaag cgcattcaga tcctgcgtga
     7921 tatgggcgtg ggtttatcgg tagatgattt tggcacgggc ttttccggat tatcccgctt
     7981 agtcagtctt ccggtaacgg aaatcaaaat tgacaaaagc tttgtcgatc gttgtctgac
     8041 ggaaaaacgc atcctggcct tacttgaagc cattaccagc attgggcaaa gcctcaattt
     8101 aaccgtcgtg gcggaagggg tcgaaaccaa agagcaattt gagatgctac gtaagatcca
     8161 ctgtcgcgtt attcagggat atttcttttc ccgccccctt cccgccgaag aaattccagg
     8221 ctggatgagc agcgtgttac cgctgaaaat ctgacaaatt cctctcgccc gcactcgcgg
     8281 gttttccttt aaggtgacac tgtcacttaa atactgtgat ttcagctacg attccgggag
     8341 attccttcct taactttccc tcttgcacca aaactgcact acgctgcaca aatgttgatc
     8401 aaaagttaaa aaatataact ttaagccagc attctggcgc ttatcccggc atggcatgag
     8461 atctgcataa gcggaaagcg cagcaatttt tgtcttatac aggaaccgtt tatgtcggat
     8521 accaccgaac tggttgattt agccgtgatc ttccctgatc tggagatcga attgaaatat
     8581 gcctgcgttg ataacatcac aggtaaagct atttatcagc aagcgcgttg tctgctacat
     8641 aaagatgcga ttaccgcgct ggcgaaaagt atcagtatcg cccagctgtc aggcttacaa
     8701 ctggtgattt acgatgcgta tcgcccacaa caagcacagg cgatgttgtg gcaagcctgc
     8761 ccagacccgc aatatgttgt tgatgtgacg gtcggttcta atcacagccg tggcacggcg
     8821 ctcgacctga cgcttcgtga tgacgtggag gtggtgaatg atgctaagcg aggaaacgtc
     8881 cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca aaactggtat ggatgctgaa
     8941 aggcagtccg ctcaccgtga ccggcgcagt catcattgta ttaatgcgac tgatgatgat
     9001 tttttcaccg tggctggcga cgcatgatcc caacgccatt gatttaaccg cccgcctttt
     9061 gccgccttct gcggcgcact ggtttggcac cgatgaagtg ggacgcgatc tgtttagccg
     9121 cgtactggtc ggcagtcagc aatcaattct cgccggatta gtggtggtcg ccattgcggg
     9181 tatgattggt tcgctactcg gatgtctatc cggtgtgctt ggcggacgcg cagacgccat
     9241 catcatgcgc atcatggaca ttatgctgtc gattccttcg ttggtactga caatggcact
     9301 ggctgccgct ctcgggccga gtttatttaa cgccatgctg gcgattgcta ttgtgcgaat
     9361 tcccttttat gtgcgcttgg cgcgggggca agcattagtt gtacgccagt atacctatgt
     9421 tcaggcggcg aaaacctttg gcgcgtctcg ttggcatctg atcaactggc atattttacg
     9481 taactcccta ccgccgctga tcgtgcaggc atcgctggat atcggtagcg cgattttaat
     9541 ggccgccacg ttgggattta ttggcctggg tgctcaacaa ccgagtgctg aatggggcgc
     9601 gatggtagcg aatggtcgca actatgtact cgatcagtgg tggtattgcg catttccggg
     9661 ggcagcgatt ttgcttacct ccgtcgggtt taatctcttt ggtgatggta ttcgcgatct
     9721 gcttgacccg aaagcaggag gaaagcagtc atgacccaac ccgttctgga cattcaacag
     9781 ctgcatttga gtttccccgg ttttaacggt gatgttcacg cgctcaacaa tgtgtccttg
     9841 cagattaacc gcggcgaaat tgtcggtctg gtgggagaat ccggctcagg taaatcagtc
     9901 accgcaatgc tgattatgcg tctgctaccg acgggcagtt attgcgtaca tcgggggcag
     9961 atttcactgc tgggagaaga tgttcttaac gctcgggaaa aacagcttcg tcagtggcgc
    10021 ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg cccttaatcc gacacgtcga
    10081 ataggtcttc agatgatgga cgtaatccgc catcatcaac caataagtcg tcgggaagcc
    10141 agagctaaag cgattgcact gctggaagag atgcaaatcc cggatgccgt ggaagttatg
    10201 tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc gggtaatgat tgcgccggca
    10261 ttctcctgcg agccgcaatt gattattgcc ggcgaaccga cgacggcgct ggacgtcaca
    10321 gtacagttgc aggtactgcg tttgcttaaa cataaagcac gcgccagtgg aactgcggta
    10381 ctgttcatca gccatgatat ggccgtggtg tcgcaactgt gcgatagcgt ttacgtgatg
    10441 tatgccggaa gcgtaattga aagcggcggg acggcagacg ttatccatca tccccggcat
    10501 ccgtatacca ttggtttgct gcaatgcgca ccagaacatg gagtaccacg ccagccatta
    10561 cccgccattc cagggacggt accaaacctc acccatttgc ctgacggctg cgcttttcgc
    10621 gatcgttgct atgcggcagg tgcacagtgt gcgctgacag cgtgtggtga caacaaccag
    10681 cactgcacct gttggtatcc gcagcaggag gtcataagtg tctgacacgt tattaacgtt
    10741 acgcgacgtc catatcaatt ttccggcccg taaaaactgg cttggcaaat ctacggaaca
    10801 tgttcatgcc attaatggta ttgatttaca gatccgccgt ggtgaaacct tagggatcgt
    10861 cggcgagtca ggctgcggca aaagcaccct cgcacagctt ttaatgggca tgctgcaacc
    10921 gagccacggg cagtacatcc gttcaggctc acaacgcatt atgcagatgg tgtttcagga
    10981 cccgctctct tcgcttaatc cgcgcttacc ggtgtggcgc atcatcacag aatcgctctg
    11041 gatagctaag cgtagtagtg aacaacagcg gcgagcgttg gcagaggagt tggctgtgca
    11101 ggtgggtatt cgtccggagt atctcgaccg cctgcctcat gcgttctccg gcgggcagcg
    11161 gcaacgcatc gccattgcca gagcactctc ttcgcagcct gacgtgattg tgcttgatga
    11221 gccaacctcg gcgctggata tctccgtgca ggcgcagatc ctcaatttgc tggtaacgct
    11281 acaggaaaat cacgggctga cttatgtgct gatttcacac aatgtctcgg tgatacgtca
    11341 tatgagcgat cgggtggcgg tgatgtatct cgggcagatt gtagaactgg gcgacgcgca
    11401 acaggtgttg actgcttcgg cacatccata cacccgatta ttgctggatt ccctccccgc
    11461 cattgataaa ccgctggagg aagaatgagc attacgtaaa acggatctgc cgggaaaccg
    11521 cacgttgccg caaggctgtt ttttctacga acgttgccct ctggcaaccc acggatgtga
    11581 agtccggcaa tcattagcaa taagggagga cggacgtgac ctccggtgct ggcgggcgct
    11641 gtagagatgc taacaacgca tcaggcattc acgcgcctga cgcgtcatcc ggcaatgctt
    11701 tacgatttca actggtaatc cagcgtaatt tccgctttca gtacctgaga gaccgggcat
    11761 cctgcttttg ctttctggat gatgccgtca aaggtagagg catcaatacc cggcaccgca
    11821 acttcactct tcagtgcgat tttcgtaatc gcaaaaccgg catccacttt atccagcgac
    11881 acatcggcgg tggtatcaat tgatgttggc gtgaatcccg cttcccccag cattaatgaa
    11941 agcgccattg agaaacatgc ggcatgcgct gcgccaatca gttcttcagg gttggttcct
    12001 ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt ggttcagcac gccgctctcg
    12061 gtggatactg ttcccttccc gcgtttgata tcgccttccc agtgtgcctg acctttctta
    12121 tggattgtca ttgttgctct cctgtgggct taaaaaggaa attatagact acgtgagaaa
    12181 cagcacgagg ataagcagga tattccgaat aaaatacggc gaaaatatta cttctgcact
    12241 taaattaaat tcccacaata gataaccggg aatatatatc tcaaga
//
LOCUS       DAZOZX010000114        12267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZOZX010000114 DAZOZX010000000
VERSION     DAZOZX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.114"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1177)
                     /locus_tag="ACHUPT_003124"
     CDS             complement(<1..1177)
                     /locus_tag="ACHUPT_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0820580.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHUPT_003125"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHUPT_003125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUPT_003126"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUPT_003126"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0820581.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHUPT_003127"
     CDS             2804..4306
                     /locus_tag="ACHUPT_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0820582.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUPT_003128"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUPT_003128"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0820583.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUPT_003129"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUPT_003129"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0820584.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUPT_003130"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUPT_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0820585.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUPT_003131"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUPT_003131"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0820586.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHUPT_003132"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHUPT_003132"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0820587.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHUPT_003133"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHUPT_003133"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0820588.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..>12267)
                     /locus_tag="ACHUPT_003134"
     CDS             complement(11871..>12267)
                     /locus_tag="ACHUPT_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR0820589.1"
                     /translation="NLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSL
                     NDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDD
                     LPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3030 a         3210 c         3290 g         2737 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattc
//
LOCUS       DAZOZX010000115        12221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZOZX010000115 DAZOZX010000000
VERSION     DAZOZX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.115"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHUPT_003135"
     CDS             complement(<1..494)
                     /locus_tag="ACHUPT_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0820590.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHUPT_003136"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHUPT_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR0820591.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHUPT_003137"
     CDS             complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHUPT_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR0820592.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2460..3326
                     /gene="folD"
                     /locus_tag="ACHUPT_003138"
     CDS             2460..3326
                     /gene="folD"
                     /locus_tag="ACHUPT_003138"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0820593.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3328..3540
                     /gene="ybcJ"
                     /locus_tag="ACHUPT_003139"
     CDS             3328..3540
                     /gene="ybcJ"
                     /locus_tag="ACHUPT_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0820594.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3648..4157
                     /locus_tag="ACHUPT_003140"
     CDS             3648..4157
                     /locus_tag="ACHUPT_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0820595.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4193..5578)
                     /gene="cysS"
                     /locus_tag="ACHUPT_003141"
     CDS             complement(4193..5578)
                     /gene="cysS"
                     /locus_tag="ACHUPT_003141"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0820596.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5752..6246
                     /gene="ppiB"
                     /locus_tag="ACHUPT_003142"
     CDS             5752..6246
                     /gene="ppiB"
                     /locus_tag="ACHUPT_003142"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0820597.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6249..6971
                     /gene="lpxH"
                     /locus_tag="ACHUPT_003143"
     CDS             6249..6971
                     /gene="lpxH"
                     /locus_tag="ACHUPT_003143"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0820598.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7089..7598
                     /gene="purE"
                     /locus_tag="ACHUPT_003144"
     CDS             7089..7598
                     /gene="purE"
                     /locus_tag="ACHUPT_003144"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0820599.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7595..8662
                     /gene="purK"
                     /locus_tag="ACHUPT_003145"
     CDS             7595..8662
                     /gene="purK"
                     /locus_tag="ACHUPT_003145"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0820600.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8799..9692)
                     /locus_tag="ACHUPT_003146"
     CDS             complement(8799..9692)
                     /locus_tag="ACHUPT_003146"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0820601.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9689..10336)
                     /locus_tag="ACHUPT_003147"
                     /pseudo
     CDS             complement(9689..10336)
                     /locus_tag="ACHUPT_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10339..11571
                     /gene="allC"
                     /locus_tag="ACHUPT_003148"
     CDS             10339..11571
                     /gene="allC"
                     /locus_tag="ACHUPT_003148"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR0820602.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11582..>12221
                     /gene="allE"
                     /locus_tag="ACHUPT_003149"
     CDS             11582..>12221
                     /gene="allE"
                     /locus_tag="ACHUPT_003149"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR0820603.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3159 c         3118 g         2978 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataaggagt
     1261 ataaagaaat gattgctttg gcttcttaca acacatcatt cccttttcat ttaataaagc
     1321 ggcatgatac aaagtgaacg tggtttcgat ttacattgcc accataccgc ttcagacaac
     1381 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
     1441 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
     1501 gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg
     1561 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
     1621 ccgacacccg ttgctgttgt tgtatttgta ctggatacaa tggccaataa attatcattg
     1681 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
     1741 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
     1801 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
     1861 gcagcattca ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa
     1921 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata
     1981 tatattcctt caatctcatt tatcgactcc acatccgtat ataaccgatt actttattta
     2041 agacactgat agtagtaaaa ttccttttta tcctctaaga atgtcttaat tgaaaatatg
     2101 cactctattc taaaaaatag agagccccgt tagatgaata cttccgcgca aaatatattc
     2161 accacaaata tagacctgaa gcggtaaatt accaggctga aaattctttt tatattgtca
     2221 ggtatttctt aaattatctt aatccttaaa caaggaaata aatcagttcc agatttacaa
     2281 cgccatcatg tacgaaaaat gaagctttca atctcagcga cctcagcgac ggtgcgccta
     2341 accttcgcaa gaggtcgctt cacgcgataa atctgaaacg aaacctgaca gcgcgccccg
     2401 cttctgacaa aatagacgca tccccttcga cctacgtaac agatggaatc ctctctctga
     2461 tggcagcaaa gattattgac ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc
     2521 aaaaagttca ggctcgtatt gcagccggac tgcgggcacc aggactggcc gttgtgctgg
     2581 tgggtagtaa ccctgcatcg caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag
     2641 tcgggttcgt ctcccgctct tatgacctcc cggaaaccac cagcgaagcg gagctgctgg
     2701 agcttatcga tgcgctgaat gccgacaaca ccatcgatgg cattctggtt caactgccgt
     2761 taccggcggg tattgataac gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg
     2821 acggtttcca tccttacaac gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct
     2881 gcaccccgcg cggtatcgtc acgctgcttg agcgttacaa cattgatacc ttcggcctca
     2941 acgccgtggt gattggcgca tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc
     3001 tcgcaggttg caccaccacc gtgactcacc gcttcactaa aaatctgcgt catcacgtag
     3061 aaaatgccga tctgttgatc gttgccgttg gcaagccagg ctttattccc ggtgactgga
     3121 tcaaagaagg cgcaattgtg attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg
     3181 tgggcgacgt cgtgtttgaa gacgcggcta aacgcgcctc atacattacg cctgttcccg
     3241 gcggcgttgg cccgatgacg gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg
     3301 aatatcatga tccacagggt gagtaacatg gcgacatttt ctttaggtaa acatccgcac
     3361 gttgagctgt gcgacttgct gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa
     3421 atcgcgattg ccgaaggcca ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc
     3481 aaaatcgtcg ccggtcagac agtgagtttt gcaggacaca gcgtacaggt tgttgcctga
     3541 tccctttccc ggcctgtatc aacaggccgg ttctcttttc agacttttgc cctctacgcc
     3601 ccgttcatcc atgctgatag catcgacatt ttgccgaagc gaccatcatg ccaaccgtta
     3661 ttacgcacgc cgccgttccc ctttgcattg gtttagggtt gggttcgaaa ggtctgttat
     3721 ttgccggaat catcctggcg atgctgcccg atgccgacgt attgtcgttt aaatttggcg
     3781 ttgcttacgg caatgttttt ggtcatcgcg gttttaccca ttcgctggtg cttgcgtttg
     3841 ttgtcccgct cttatgtgtg ctgattggac gtcgatggtt cagggctggg ctgattcgct
     3901 gctggctgtt tttaaccgtc tcgttgctat cgcacagctt actggattcg gtaaccactg
     3961 gcggtaaggg cgttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc
     4021 aggtgattaa agtcgcgccg tttgcattgt ctcgttacac cacagcgtac gggcatcagg
     4081 tgattatttc cgaattgatg tgggtgtggt tgccggggat gctactgatg ggaatgttgt
     4141 ggtggcgcag acgataaccg gatgcgaaaa ctcgcatccg gcaattgcgc aattacttac
     4201 gacgccaggt ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt
     4261 cacgtgccgc atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa
     4321 ttaacgcttc aatctcagcc acttcgctgt cgtctgcctg cgcgccgctt tgcaggaacg
     4381 cttccggttc ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagatgcca
     4441 ttgcattcgc cgctgccata tcttctgctt tcagacggtt cacttcacgc gccatatcaa
     4501 acagcacgga ataggcttcc ggggtgttga aatcgtcgtc catcgcttca ataaagcgcg
     4561 cttcaaacgc ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc agcgcagtgt
     4621 agaggcgctc cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag ttcagctggc
     4681 tgcgatagtg gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag tatttcagca
     4741 catcgcgcac ggtaaagaag ttacccagcg atttggacat cttctcgcgg tcaaccatca
     4801 ccattccgga gtgcatccag tagttcacat actgaccatc atgggcacag gtggactgcg
     4861 cgatttcgtt ctcgtggtgc gggaacatca ggtctgaacc gccgccgtgg atatcaaagt
     4921 ggttacccag ctgcttgcag ttcatggccg aacattcaat gtgccagcca ggacgacccg
     4981 cgccccacgg agacggccag ctcggttcgc cctctttcga catcttccac agaacgaagt
     5041 ccatccggtt gcgtttgtca tcaaccacgt caacgcgcgc gccagcctgc agctggtcga
     5101 gatcctgacg cgacagcacg ccataagttg gatcggtcgg gacgtcgaac atcacgtcgc
     5161 tgttgtccgc cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt
     5221 ctgcgatatg gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa
     5281 aatctttgtg catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt
     5341 cattggcgcg tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca tacttcagtt
     5401 tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag gtacgcccgt
     5461 gaccgatatg acagagatcg taaacggtga ttccacacac gtacatgccg acttccccgg
     5521 cgtgaatagg cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg
     5581 aagattccgt ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg
     5641 cgaatcagca tacattgcgt gatgatccga tcgtgcggtt atgctataac accaccctat
     5701 atatgacccg aactgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact
     5761 ttccacacca atcacggcga tattgtcatc aaaacttttg acgataaagc acctgaaaca
     5821 gttaaaaact tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt
     5881 gttatcaacg gctttatgat tcagggcggc ggttttgaac cgggcatgaa acaaaaagcc
     5941 accaaagaac cgatcaaaaa cgaagccaac aacggcctga aaaatacccg tggtacgctg
     6001 gcaatggcac gtactcaggc tccgcactct gcaactgcac agttcttcat caacgtggtt
     6061 gataacgact tcctgaactt ctctggcgaa agcctgcaag gttggggcta ctgcgtgttt
     6121 gctgaagtgg ttgacggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt
     6181 aacggtatgc accaggacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc
     6241 gagtaatcgt ggcgacactc tttattgcag atcttcatct ctgcgtggaa gaaccggcga
     6301 tcaccgccgg ttttctgcgt tttttagcgg gggaagcccg caaggccgac gcgctgtata
     6361 ttcttggcga tctgtttgaa gcatggattg gcgacgacga tcccaaccca ctccatcgcc
     6421 agatggcggc agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg
     6481 gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acattattgc
     6541 cggaagaaaa ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat ggcgacacgc
     6601 tgtgcaccga tgacgcgggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc
     6661 aaatgctatt cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgcg
     6721 cgaacagcaa agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg
     6781 ctgtggtcag tgcgatggaa aaacatcagg tgcaatggct aatccacggg catacccatc
     6841 gcccggcggt gcatgaactt atcgccaacc agcaacctgc ttttcgcgtg gtactgggtg
     6901 cctggcatac ggaaggttcg atggtgaaag tcacggctga tgacgttgag ctgattcatt
     6961 ttccgtttta aaaaacccac aactttgctg atttcacagc cacgcaaccg ttttccttgc
     7021 tctctttccg tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt
     7081 taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa
     7141 gcgactgggc taccatgcag ttcgccgtcg aaatcttcga aatcctgaat gtcccgcacc
     7201 acgttgaagt ggtttctgct caccgcaccc ccgataaact gttcagcttc gccgaaagcg
     7261 ccgaagagaa cggttatcag gtgattattg cgggcgcagg cggcgcagcg cacctgccag
     7321 gcatgattgc cgccaaaacg ctggtgccgg tgctgggcgt gccagtacag agcgccgcac
     7381 tgagcggtgt cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta
     7441 cgctggcgat tggtaaagct ggcgcggcaa acgcggcatt actggcagca caaattctcg
     7501 cgactcacga taaagagcta caccagcgtc tgaatggctg gcgcaaagcc cagaccgacg
     7561 aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa
     7621 cgggcagtta ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg ctgtctggcc
     7681 ggtcgggctg gacgctgaac cggcggcggt gccttttcaa caaagcgtga ttaccgctga
     7741 gatcgaacgc tggccggaaa ccgcattaac ccgcgagctg gcgcgtcatc cggcctttgt
     7801 gaaccgcgat gtgttcccga ttattgctga ccgtctgact cagaaacagc ttttcgataa
     7861 gctccacctg ccgaccgcgc cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt
     7921 gtttgatcgt ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg
     7981 cggtcaatgg agtttacgcg ccaatgaaac cgaacagtta ccggcagagt gttacggtga
     8041 atgtattgtc gagcagggca ttaacttctc tggtgaagta tcgctggttg gcgcgcgcgg
     8101 ctttgatggc agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt
     8161 gcgcaccagc gtcgcttttc cgcaggccaa cgcacagcag caggcgcaag ccgaagagat
     8221 gctgtcggcg attatgcagg agctgggcta tgtgggcgtg atggcgatgg agtgttttgt
     8281 caccccgcaa ggtctgctga tcaacgaact ggctccgcgt gtgcataaca gcggtcactg
     8341 gacacaaaac ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct
     8401 gccgttaccg caaccagtag tgaataatcc gtcggtgatg atcaatctga ttggtagcga
     8461 tgtgaattat gactggctga aattgccgct ggtgcatctg cactggtacg acaaagaagt
     8521 ccgtccgggg cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac
     8581 tgcgacgctg gaagccttaa tcccgctgct gccgccagag tatgccagcg gcgtgatttg
     8641 ggcgcagagt aagttcagtt aagcaggtgc tcatttgatt gccggatgcg gcgtaaacgc
     8701 cttatccggc ttaccgctcc ggtacccatt gtaggcctga taagacgcgt caagcgtcgc
     8761 atcaggcatg gtgcaccgat tgccggatgc tctcatcact acagcgaaat acaggtcccc
     8821 gcttcgcccg ccagcgtctc ttcaattcgc gataacgccc caatccacgc gggtttacta
     8881 cggcttctga cataaccact caccgccgtt accttcggcc ccatcgaacc atcggctttg
     8941 gcaaatggcg ctaactcatc cggtgtggca tggcgaatgg cacgttgctg cggcgttccc
     9001 cagttttcat ataccgcatc agcatcggtg aggatcacca gtccatcggc attaatctgc
     9061 tcggcgagca acgcagcggc gagatcttta tcaatcaccg cttcactccc tgccccatct
     9121 tccgtcacag gcacaccgcc gccgccactg caaatcacca catgcccctc tttgagcaac
     9181 aactcgatgg cttcgctgtc gagaatttta cgcggttgcg gagacgccac cacccggcgc
     9241 aaatatttac catcacgttt catctgccag ccgtaagccg cttccagtgc ctcttgttct
     9301 tctggctgat aaaccggacc aataaatttc tctggctgca aaaacgccgg atcatcaggc
     9361 gaaacctcaa tgcgcgtcag caccgtcgta acaggcggca tctgcggctg tgcgctcaag
     9421 ctctgcgcca gcatatagcc aatcatcccc tggctttccg ccaccagtac atccagcgga
     9481 tacggttcta cctctttcca cgccagattc tgtaacgcca gcagccccac ctgcggcccg
     9541 ttgccgtgaa caatcgccag ccgataagaa cgggccaggc gtgccagcgc gggtacagca
     9601 ctggcgatat tgcgatattg attttccgcc gtcagcgcct caccgcgctg gagtaaggcg
     9661 ttgcccccaa gagctacaac cagtgttttc atggttttcc ttgtaataat tgttggccaa
     9721 gccagaaccc cagcagcgta tctgcccctg acgtatgccc cagcgccagc agcgaatcaa
     9781 tcgcaacagc ggtacgtttc gggcaactca gagcatggac aaagtgcagg agtggcgagg
     9841 cgaaatatcc ttgcgcggca taacgtaaat aactgacgct caccgctgtg gtaacgagtt
     9901 gaagattgtc ggaacaggca aaaaacgggc gaccggcgcg cgcatctaaa gcgccataat
     9961 accaggccgc cagcagcatt ccgctcaacg tgtcatcatg acttggcgtt aatccggggc
    10021 ctttacccag ccagtgccgc cagtcggtct taacgccatt gagcgcggcc tgaaaacagt
    10081 gacgaaactg gcgtaattca gcgggcagcg gatcgcttgc cgccatcgcc agtggcccga
    10141 aaagtccggt ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgcgaag
    10201 gcggcgtaat acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc
    10261 gaatcccttg agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat
    10321 cgaattgcgt gcggcgaaat tacacattcc cgtcaagcta tagaagaaac gctgccctgg
    10381 ctttcctctt ttggcgctga cccaacgggt gggatgaccc gtttacttta ttcgccggaa
    10441 tggctggaaa cccagcagca atttaaaaaa agaatggcag caagtgggct ggaaacacgt
    10501 ttcgatgaag tggggaattt atacggtcgc ctgagtggca ccgaatatcc acaggaagtg
    10561 gttctgagcg gttcgcatat cgataccgtg gttaacggcg gtaacctcga cgggcaattc
    10621 ggcgcgctgg cggcgtggct ggcaattgac tggttgaaaa cgcaatacgg cgcaccgtta
    10681 cgtacggtcg aagtggtggc gatggcagaa gaagaaggca gccgcttccc gtatgtcttc
    10741 tggggcagta aaaatatctt tgggctggcg aatcctgacg acgtgcggaa tatctgtgat
    10801 gccaaaggaa atagttttgc cgatgcgatg aaggcttgcg gatttactct gccgaacgcc
    10861 ccactaactc cgcgtcaggc tataaaagcc tttgttgaac tgcatattga acagggctgt
    10921 gtgctggaaa gtaatgggca atcaattggc gtggtgaatg caattgtcgg gcagcgtcgc
    10981 tatacggtga cgctgaacgg cgaatcaaac catgcaggca ccacgccgat gggttatcgt
    11041 cgtgatacgg tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag
    11101 atgggcgatc cgctggttct gacctttggc aaagtagagc cgcgcccgaa tacggtaaat
    11161 gtggtgccgg gtaaaaccac gttcaccatt gattgtcgtc ataccgacgc tgccgtgctg
    11221 cgcgatttca cccaacagtt agaaaacgac atgcgggcga tttgcgatga aatggacatt
    11281 ggtattgata tcgatttatg gatggacgaa gaacccgtgc cgatgaataa ggagctggtc
    11341 gccaccctga cagaattgtg tgaaagagaa aaactgaatt accgggtgat gcacagtggt
    11401 gccgggcacg acgcgcaaat tttcgcgcct cgcgtaccaa cctgcatgat ttttatcccc
    11461 agcatcaacg ggatcagcca taacccggcg gaacgcacca atattactga ccttgccgaa
    11521 ggggtcaaaa cgttggcact catgctttat caacttgcct ggcaggaata aggagtcata
    11581 aatgggatat ttaaataacg tcaccggtta ccgcgaagat ttactggcta accgtgcgat
    11641 tgttaaacac ggtaatttcg cactgttaac cccggatggt ctgataaaaa atattattcc
    11701 ggactttgaa aattgtgacg cgacaatcct ctccacgcca aagctgggtg cctcttttgt
    11761 tgattatctg gtcacactgc atcaaaacgg tggcaaccaa cagggcttcg gtggcgaagg
    11821 cattgaaacg ttcctgtatg tgatctctgg aaatatcact gccaaagccg aaggcaaaac
    11881 atttgcctta agcgaaggtg gctatcttta ttgcccacca ggctccttaa tgacgtttgt
    11941 taacgcccag gccgaagaca gccaaatctt tttatataag cgccgctatg ttccggtaga
    12001 aggccatgca ccgtggctgt tttctggcaa tgccagcgaa ctggaacgca ttcactatga
    12061 aggtatggat gatgtgatcc tgctggattt tctgcccaaa gagttaggtt ttgatatgaa
    12121 catgcatatc ctctcttttg caccaggtgc cagccacggt tatatcgaaa cacacgttca
    12181 ggaacatggt gcctatattc tttccggtca gggggtttat a
//
LOCUS       DAZOZX010000116        12154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZOZX010000116 DAZOZX010000000
VERSION     DAZOZX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.116"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            126..995
                     /locus_tag="ACHUPT_003150"
     CDS             126..995
                     /locus_tag="ACHUPT_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0820604.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHUPT_003151"
     CDS             complement(1198..1551)
                     /locus_tag="ACHUPT_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0820605.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUPT_003152"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUPT_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0820606.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHUPT_003153"
     CDS             complement(3379..3672)
                     /locus_tag="ACHUPT_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0820607.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUPT_003154"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUPT_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0820608.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHUPT_003155"
     CDS             complement(5220..5696)
                     /locus_tag="ACHUPT_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0820609.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUPT_003156"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUPT_003156"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0820610.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUPT_003157"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUPT_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0820611.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUPT_003158"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUPT_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0820612.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUPT_003159"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUPT_003159"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0820613.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHUPT_003160"
     CDS             11052..11627
                     /locus_tag="ACHUPT_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0820614.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHUPT_003161"
     tRNA            11734..11809
                     /locus_tag="ACHUPT_003161"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caat
//
LOCUS       DAZOZX010000117        12114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZOZX010000117 DAZOZX010000000
VERSION     DAZOZX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.117"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..371
                     /locus_tag="ACHUPT_003162"
     CDS             <1..371
                     /locus_tag="ACHUPT_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820615.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHUPT_003163"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHUPT_003163"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0820616.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHUPT_003164"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHUPT_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0820617.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHUPT_003165"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHUPT_003165"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0820618.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHUPT_003166"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHUPT_003166"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0820619.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHUPT_003167"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHUPT_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0820620.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHUPT_003168"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHUPT_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR0820621.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHUPT_003169"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHUPT_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0820622.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHUPT_003170"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHUPT_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0820623.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHUPT_003171"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHUPT_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0820624.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHUPT_003172"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHUPT_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0820625.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHUPT_003173"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHUPT_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0820626.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12114)
                     /locus_tag="ACHUPT_003174"
     CDS             complement(11913..>12114)
                     /locus_tag="ACHUPT_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0820627.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2785 a         3388 c         2933 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atatccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aaga
//
LOCUS       DAZOZX010000118        12083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZOZX010000118 DAZOZX010000000
VERSION     DAZOZX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.118"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            278..1048
                     /locus_tag="ACHUPT_003175"
     CDS             278..1048
                     /locus_tag="ACHUPT_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0820628.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1039..1512
                     /locus_tag="ACHUPT_003176"
     CDS             1039..1512
                     /locus_tag="ACHUPT_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0820629.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1619..2158
                     /gene="dnaT"
                     /locus_tag="ACHUPT_003177"
     CDS             1619..2158
                     /gene="dnaT"
                     /locus_tag="ACHUPT_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0820630.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2161..2898
                     /gene="dnaC"
                     /locus_tag="ACHUPT_003178"
     CDS             2161..2898
                     /gene="dnaC"
                     /locus_tag="ACHUPT_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0820631.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2947..3441
                     /gene="yjjA"
                     /locus_tag="ACHUPT_003179"
     CDS             2947..3441
                     /gene="yjjA"
                     /locus_tag="ACHUPT_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0820632.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3681..5972
                     /gene="opgB"
                     /locus_tag="ACHUPT_003180"
     CDS             3681..5972
                     /gene="opgB"
                     /locus_tag="ACHUPT_003180"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0820633.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6110..7132)
                     /gene="lgoD"
                     /locus_tag="ACHUPT_003181"
                     /pseudo
     CDS             complement(6110..7132)
                     /gene="lgoD"
                     /locus_tag="ACHUPT_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7271..8185
                     /locus_tag="ACHUPT_003182"
     CDS             7271..8185
                     /locus_tag="ACHUPT_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0820634.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8400..9761
                     /locus_tag="ACHUPT_003183"
     CDS             8400..9761
                     /locus_tag="ACHUPT_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0820635.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9810..11474)
                     /gene="tsr"
                     /locus_tag="ACHUPT_003184"
     CDS             complement(9810..11474)
                     /gene="tsr"
                     /locus_tag="ACHUPT_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR0820636.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11593..12039)
                     /gene="hpaR"
                     /locus_tag="ACHUPT_003185"
     CDS             complement(11593..12039)
                     /gene="hpaR"
                     /locus_tag="ACHUPT_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0820637.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2953 a         3187 c         2927 g         3016 t
ORIGIN      
        1 gatgatttta acttgttaac actaaaaaac gcgcccagct atctattatc ctgacccgta
       61 aaggctaaac aataaattca ttatagtgat aatattcatg tgatagtttg tttgcggcga
      121 gagataattc gctttttatc accgagcttg tcccaataac caggattaat cagcgcaatt
      181 ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata caaatggatt ctcattatct
      241 gaacaatacg cagcacgtct atgacaaagg gcgagttatg caaactgagc aacagcgagc
      301 cgtaacgcgg ctttgtatcc agtgtggatt atttcttttg caacatggtg cggaaagcgc
      361 gttggttgat gagctttcct cacgactggg tcgggcgctg ggaatggaca gcgtcgaaag
      421 ttctatctct tcgaacgcca tagtgctgac aactattaaa gatgggcaat gcctgacatc
      481 gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg gtgactgaag tccagcacat
      541 tgtgattctt gcggagcatc atctgctgga ttacaaaggc gtagagaaac gatttagcca
      601 aattcaacca ttacgttacc caagatggct ggtagcctta atggttggcc tttcttgcgc
      661 ctgtttctgt aaactcaata acggtggctg ggatggtgcc gtcatcacct tctttgccag
      721 tacgaccgcg atgtatatcc gccagctgct ggcacaacgt catcttcatc cacagatcaa
      781 cttttgcctt accgctttcg ccgccaccac catttccgga ttgcttttgc aactccccac
      841 tttcagcaat acccccacca ttgcgatggc cgccagcgtt ctgctgctag tgccgggctt
      901 tccgttgatt aatgccgtcg ccgatatgtt taaaggccac atcaataccg gactggcacg
      961 ctgggcgatc gccagtctgc tgacactggc tacctgcgtc ggcgtagtga tggcactgac
     1021 gatttggggg ctacgcggat gggtgtgatc gaatttctgt tagcgttggc gcaggatatg
     1081 atcctcgccg ccattcctgc ggtcggcttt gcgatggtgt tcaacgttcc cgtgcgggcg
     1141 ttacgctggt gtgcgctgct tggctcgata ggtcatggtt cccgaatgat cttgatgacc
     1201 agcgggttga atattgagtg gtcaaccttt atggcttcta tgctggtcgg taccattggt
     1261 attcaatggt cgcgctggta tctggcgcat ccgaaagtgt ttaccgtggc ggccgttatc
     1321 cctatgttcc cgggcatatc ggcttatacc gcaatgattt cgtcggtaaa aatcagccag
     1381 ttaggttaca gcgaaccgtt gatgattacc ctgttaacca actttcttac agcttcatcg
     1441 attgttggtg cgttatccat cggtctttcc attcctggat tatggttgta ccgcaagcgc
     1501 cctcgcgtat aaaattgcct cgcttaagca cacggatgag agacagcctc ctctcctccg
     1561 tgtgttacta taaaagttat ctcccttctc gttcatcgtt ccatatttga gaaacagtat
     1621 gtcttccaga gttttgaccc cggacgtcgt tggtattgac gccctggtac acgatcacca
     1681 aaccgttctg gcaaaagctg aaggcggtgt ggttgccgta tttgctaaca atgccccggc
     1741 gttttatgcc gtcacgcctg cacgcctggc tgaactgctg gcgctggaag aaaagctagc
     1801 gcgtccggga agcgatgtcg ctctggacga tcaactctat caggaaccgc aagccgctcc
     1861 cgttgctgta cccatgggga aattcgccat gtatccggac tggcaacccg atgccgattt
     1921 tatccgcctg gcggcgctat ggggcgtggc gctaagagag ccggtgacca ccgaagaact
     1981 ggcctcattc attgcctact ggcaggcgga aggtaaagtc tttcaccatg tgcagtggca
     2041 acaaaaactg gcgcgcagcc tgcaaatcgg tcgtgccagc aacggcggac tgccgaaacg
     2101 agatgtgaat acggtcagtg aacctgacag ccaaattcca ccaggattca gagggtaacg
     2161 atgaaaaacg ttggcgacct gatgcaacgc ctgcaaaaaa tgatgcctgc ccatatcaaa
     2221 cctgcattca aaacgggtga agagctactg gcctggcaga aagaacaagg ggcgatccgc
     2281 tccgccgctc tcgaacgtga aaatcgggcg atgaaaatgc agcgcacctt taaccgctcc
     2341 ggtattcgtc cactgcatca gaactgctcc tttgagaact atcgcgttga gtgtgaaggg
     2401 cagatgaatg cgttaagcaa agcgcgccag tatgtcgaag agtttgacgg caacatcgcc
     2461 agcttcatct tttctggtaa gccaggaacc ggcaaaaacc atctggcggc ggcaatctgc
     2521 aacgagctgc tgctacgcgg taaatccgta ttgatcatca ccgtggccga tattatgtcg
     2581 gcgatgaaag ataccttcag gaatagcggt accagcgaag aacaactgct taacgatctg
     2641 agcaacgttg atctgctggt gatcgatgag atcggcgtgc agaccgaatc gaaatacgaa
     2701 aaagtgatca tcaaccagat cgtcgatcgc cgctcttctt ccaaacgccc aaccgggatg
     2761 ctgaccaaca gtaatatgga agagatgacc aagttgctgg gcgaacgcgt gatggaccgt
     2821 atgcgcctgg gtaacagttt atgggtgatc ttcaactggg atagctaccg tagccgggta
     2881 acgggtaaag agtattaatc cccgtttctg gcggtatact gcgacacaat tccaccttga
     2941 gtaatgatga tgaaaactat taaacatctt ctgtgctgtg ccattgccgc cagcgcatta
     3001 atttccaccg gggtgcatgc tgcgtcctgg aaagatgcgc tctccagcgc cgccagcgaa
     3061 cttggcaacc aaaacagcac gacacaggaa ggcggttggt cgctcgcgtc attaactaac
     3121 ttgcttagca gcggaaacca agccttaagc gcagataaca tgaacaatgc cgcaggcatt
     3181 ctgcaatact gcgcgaagca aaagctggct tcggtaaccg atgccgaaaa catcaagaac
     3241 caggtgctgg aaaagctggg cctgaacagt gaagagcaaa aagaagacac caactatctg
     3301 gacggtattc agggtttgct gaaaacaaaa gatggtcagc aactcaatct ggataacatc
     3361 ggaacgactc cgctggcaga aaaggtgaaa accaaagcct gcgatctggt gttaaagcag
     3421 gggctgaact tcatttcctg aaccgcgaaa tgttacccgg ctgacgcggc tctctcctgc
     3481 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3541 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3601 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3661 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     3721 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     3781 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     3841 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     3901 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     3961 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4021 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4081 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4141 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4201 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4261 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4321 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4381 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4441 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4501 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4561 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4621 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4681 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     4741 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     4801 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     4861 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     4921 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     4981 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5041 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5101 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5161 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5221 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5281 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5341 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5401 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5461 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5521 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5581 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5641 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     5701 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     5761 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     5821 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     5881 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     5941 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6001 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6061 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6121 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6181 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6241 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6301 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6361 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6421 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6481 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6541 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6601 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6661 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     6721 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     6781 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     6841 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     6901 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     6961 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7021 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7081 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7141 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7201 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7261 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7321 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7381 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7441 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7501 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7561 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7621 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7681 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     7741 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     7801 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     7861 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     7921 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     7981 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8041 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8101 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8161 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8221 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8281 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8341 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8401 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8461 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8521 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8581 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8641 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     8701 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     8761 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     8821 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     8881 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     8941 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9001 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9061 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9121 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9181 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9241 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9301 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9361 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9421 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9481 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9541 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9601 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9661 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
     9721 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
     9781 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
     9841 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
     9901 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
     9961 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10021 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10081 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10141 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10201 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10261 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10321 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10381 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10441 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10501 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10561 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10621 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10681 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    10741 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    10801 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    10861 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    10921 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    10981 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11041 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11101 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11161 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11221 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11281 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11341 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11401 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11461 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11521 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11581 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11641 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    11701 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    11761 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    11821 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    11881 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    11941 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12001 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12061 aaaatcatta atatagaaac aat
//
LOCUS       DAZOZX010000119        11990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZOZX010000119 DAZOZX010000000
VERSION     DAZOZX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.119"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..2251)
                     /locus_tag="ACHUPT_003186"
     CDS             complement(<1..2251)
                     /locus_tag="ACHUPT_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR0820638.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACHUPT_003187"
     CDS             complement(2275..2898)
                     /locus_tag="ACHUPT_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR0820639.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACHUPT_003188"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACHUPT_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHUPT_003189"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHUPT_003189"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0820640.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHUPT_003190"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHUPT_003190"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0820641.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11919)
                     /gene="yfaL"
                     /locus_tag="ACHUPT_003191"
                     /pseudo
     CDS             complement(8356..11919)
                     /gene="yfaL"
                     /locus_tag="ACHUPT_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2777 a         3320 c         2955 g         2938 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag gccccctgct tctgattacc
     8881 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     8941 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     9001 aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac
     9061 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     9121 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     9181 aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     9241 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     9301 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     9361 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     9421 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     9481 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac
     9541 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     9601 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     9661 cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     9721 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     9781 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     9841 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     9901 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     9961 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
    10021 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
    10081 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
    10141 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
    10201 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
    10261 ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata
    10321 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
    10381 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
    10441 cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc
    10501 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
    10561 gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa
    10621 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
    10681 aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc
    10741 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
    10801 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
    10861 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
    10921 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
    10981 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
    11041 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
    11101 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
    11161 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
    11221 atacataaag ccacccgccg cagtggaggg accatctcca tagcctgctg cgctattgtt
    11281 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
    11341 atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
    11401 tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt
    11461 atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg
    11521 gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc
    11581 acctttgcca tcattagcga tattattgat ggcgttagtg acacgtaaat cgacggcacc
    11641 agtatcatta gtaccagaag aatagattgc gccaccatag ccgcctgcga cgttaccgga
    11701 aaaaataaca tcagtaagat tcagcgttga gttttcttta gcaaatatgg ccccgccgtt
    11761 attatattct cctgtaacgg tgttattggc aaacagagtc attccagttt cattttctgg
    11821 taatagtgaa aattccgctc cttgttgcaa aaatacggcc ccaccgctgg cattattggt
    11881 catatccgaa aaaaccagcc attgcccatc ggcgatagac tggccccctg aatctccaga
    11941 caaccagtat cacttattta agtgatagtc ttaatactag tttttagact
//
LOCUS       DAZOZX010000120        11793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZOZX010000120 DAZOZX010000000
VERSION     DAZOZX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.120"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            38..778
                     /locus_tag="ACHUPT_003192"
     CDS             38..778
                     /locus_tag="ACHUPT_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0820642.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHUPT_003193"
     CDS             837..1223
                     /locus_tag="ACHUPT_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0820643.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHUPT_003194"
     CDS             1232..1561
                     /locus_tag="ACHUPT_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0820644.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHUPT_003195"
     CDS             1533..4574
                     /locus_tag="ACHUPT_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0820645.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHUPT_003196"
     CDS             4574..4903
                     /locus_tag="ACHUPT_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0820646.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHUPT_003197"
     CDS             4903..5601
                     /locus_tag="ACHUPT_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0820647.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHUPT_003198"
     CDS             5606..6349
                     /locus_tag="ACHUPT_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0820648.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHUPT_003199"
     CDS             6313..6888
                     /locus_tag="ACHUPT_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0820649.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHUPT_003200"
     CDS             6949..10428
                     /locus_tag="ACHUPT_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0820650.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="ACHUPT_003201"
     CDS             10496..11095
                     /locus_tag="ACHUPT_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0820651.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11159..>11793
                     /locus_tag="ACHUPT_003202"
     CDS             11159..>11793
                     /locus_tag="ACHUPT_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0820652.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2774 a         2795 c         3790 g         2434 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11521 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11581 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11641 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11701 ggatgccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
    11761 gaccggcact ctgaaaatgg ctaaccatct gtc
//
LOCUS       DAZOZX010000121        11789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZOZX010000121 DAZOZX010000000
VERSION     DAZOZX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.121"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHUPT_003203"
     CDS             <1..851
                     /locus_tag="ACHUPT_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0820653.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHUPT_003204"
     CDS             873..2282
                     /locus_tag="ACHUPT_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0820654.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHUPT_003205"
     CDS             2260..3039
                     /locus_tag="ACHUPT_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0820655.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHUPT_003206"
     CDS             3036..3896
                     /locus_tag="ACHUPT_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0820656.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHUPT_003207"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHUPT_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHUPT_003208"
     CDS             complement(4636..4896)
                     /locus_tag="ACHUPT_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0820657.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHUPT_003209"
     CDS             complement(4887..6158)
                     /locus_tag="ACHUPT_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0820658.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHUPT_003210"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHUPT_003210"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0820659.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHUPT_003211"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHUPT_003211"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0820660.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHUPT_003212"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHUPT_003212"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0820661.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHUPT_003213"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHUPT_003213"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0820662.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHUPT_003214"
     CDS             11500..11652
                     /locus_tag="ACHUPT_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0820663.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2838 a         2993 c         3240 g         2718 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaatt
//
LOCUS       DAZOZX010000122        11786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZOZX010000122 DAZOZX010000000
VERSION     DAZOZX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.122"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(22..762)
                     /gene="cysQ"
                     /locus_tag="ACHUPT_003215"
     CDS             complement(22..762)
                     /gene="cysQ"
                     /locus_tag="ACHUPT_003215"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0820664.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            952..2895
                     /gene="cpdB"
                     /locus_tag="ACHUPT_003216"
     CDS             952..2895
                     /gene="cpdB"
                     /locus_tag="ACHUPT_003216"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0820665.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3021..3401)
                     /locus_tag="ACHUPT_003217"
     CDS             complement(3021..3401)
                     /locus_tag="ACHUPT_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0820666.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3490..4350
                     /gene="qorB"
                     /locus_tag="ACHUPT_003218"
     CDS             3490..4350
                     /gene="qorB"
                     /locus_tag="ACHUPT_003218"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0820667.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4459..5424
                     /locus_tag="ACHUPT_003219"
     CDS             4459..5424
                     /locus_tag="ACHUPT_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0820668.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5532..6194
                     /gene="ytfE"
                     /locus_tag="ACHUPT_003220"
     CDS             5532..6194
                     /gene="ytfE"
                     /locus_tag="ACHUPT_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0820669.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6239..7651)
                     /gene="cycA"
                     /locus_tag="ACHUPT_003221"
                     /gene_synonym="dagA"
     CDS             complement(6239..7651)
                     /gene="cycA"
                     /locus_tag="ACHUPT_003221"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0820670.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7959..8579)
                     /gene="fklB"
                     /locus_tag="ACHUPT_003222"
     CDS             complement(7959..8579)
                     /gene="fklB"
                     /locus_tag="ACHUPT_003222"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0820671.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8798..9343
                     /locus_tag="ACHUPT_003223"
     CDS             8798..9343
                     /locus_tag="ACHUPT_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR0820672.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9327..9974)
                     /locus_tag="ACHUPT_003224"
     CDS             complement(9327..9974)
                     /locus_tag="ACHUPT_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0820673.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10186..10977
                     /locus_tag="ACHUPT_003225"
     CDS             10186..10977
                     /locus_tag="ACHUPT_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR0820674.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            10987..>11786
                     /locus_tag="ACHUPT_003226"
     CDS             10987..>11786
                     /locus_tag="ACHUPT_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0820675.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         2970 a         3070 c         2976 g         2770 t
ORIGIN      
        1 cactgtttct gccatctgaa tttagtaaat ggacactctg aaccccggat tcaggaacga
       61 ctcgcgcgga gtgtaatcca gcggtttacc ctgccagtcg tgaacgtgcg ctccggcagc
      121 tgcagccaca gcatgtccgg cggcggtgtc ccaaatattc gttggtccga agcgcgggta
      181 cagctgcgcc tgtccttccg ccaccaggca gaatttcagc gaagagccga tggacgtggt
      241 ctgatgttcg ccaagctgtt gcagatactc tttcagctcc gcatctgcat gggaacggct
      301 gataaccacc agcggcgggc gcgcatcgcg gacctgaatc agcttgcgca caccgcactc
      361 ttctttccag gccttgcctt ctgccgcgct gtacattacg ttcattaccg gcgcatacac
      421 cacacctaaa atcggtttgc catggtcaat gagcgcaatg ttaacggtga attcgccatt
      481 acgtttaata aactctttag taccatccag cgggtctacc agccagtaac gctgccagtg
      541 ctgacggact tcccaaccgg gaggatcttc ttcagaaagg accggaatat ccggtgccag
      601 cgtacgtaaa ccgtccatga taacggtgtg agcggcaata tccgctgccg ttaccggaga
      661 attgtccgct ttgctgacga cgtccatcgg tttcgtcccg tcgtagacct gcataatggc
      721 atcgcctgca ttccgtgcaa gctggcatac ttgatctaac atttctccac ctcgtctctg
      781 tgagcggtgt taacttattg ttttacttat accctatcgt taatgaatgc gccaactgtg
      841 atagtgtcat cattttcaaa gcgtaaaatt gtggcattct tcactgttct ataagtaagg
      901 cgtttattct cccttttctt tcgtattccc gatgataaaa ggatgtccct gatgattaag
      961 tttagcgcaa cgctcctggc cacgctgatt gccgccagtg tgaatgcagc gacggtcgat
     1021 ctgcgtatca tggaaaccac tgatctgcat agcaacatga tggatttcga ttattacaaa
     1081 gacaccgcca cggaaaaatt cggactggta cgtacggcaa gcctgattaa cgatgcccgc
     1141 aatgaagtga aaaacagcgt actggtcgat aacggcgatt tgattcaggg gagtccgctg
     1201 gccgattaca tgtcggcgaa aggattaaaa gcaggtgatg ttcatccggt ttataaggcg
     1261 ctgaatacgc tggattatac ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg
     1321 gattacctga aaaatgcgct ggcgggagcg aaattccctt atgtaaatgc caacgtcatt
     1381 gacgccagaa ccaaacagcc aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc
     1441 gataaagacg gaaaaaaaca gacgctgaag attggctata ttggcgtcgt gccgccgcaa
     1501 atcatgggct gggataaagc taatttatcc ggaaaagtga cggtgaatga tattaccgaa
     1561 accgtgcgca aatacgtgcc tgaaatgcgc gagaaaggtg ccgatgtcat tgtcgttctg
     1621 gcgcattccg ggctgtctgc cgatccgtat aaagtaatgg cggaaaactc agtttattac
     1681 ctcagtgaaa ttccgggcgt taacgccatt atgtttggcc atgctcacgc cgttttcccg
     1741 ggtaaagatt ttgctgatat cgaaggggct gatatcacca aaggcacgct gaatggtgtt
     1801 ccggcggtaa tgccgggcat gtggggcgat catcttgggg tggtcgactt acaactcagt
     1861 aatgacagcg gtaaatggca ggtgacgcag gcgaaagcgg aagcacggcc gatttacgac
     1921 atcgccaata aaaaatccct cgcggcggaa gacagcaagc tggtagaaac actcaaagcc
     1981 gatcacgatg ccacacgcca gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg
     2041 tatagctatc tggcgctggt gcaggacgat ccgaccgtgc aagtcgtgaa caacgcgcaa
     2101 aaagcgtatg tcgaacatta cattcagggc gatccggatc tggcaaaact gccggtgctt
     2161 tcagctgccg caccgtttaa agttggtggt cgcaaaaatg acccggcaag ctatgtggag
     2221 gtggaaaaag gccagttgac cttccgtaat gccgccgatc tttatctcta ccccaatacg
     2281 ctgattgtag tgaaagccag cggtaaagag gtgaaagagt ggctggagtg ctccgccgga
     2341 cagtttaacc agattgctcc taacagcacg aaaccacagt cactcatcaa ctgggatggt
     2401 ttccgcactt ataactttga tgttattgat ggtgtgaatt atcagattga tgttacccaa
     2461 cccgcccgct atgacggcga gtgccagatg attaatgcca atgcagagcg gattaagaac
     2521 ctgaccttta atggcaagcc gattgatccg aacgctatgt tcctggttgc caccaataac
     2581 tatcgcgctt acggcggcaa atttgccggt acgggcgaca gccatatcgc ttttgcttca
     2641 ccggatgaga accgctcggt gctggcagcg tggattgctg atgagtcgaa acgtgcgggg
     2701 gaaattcatc cggcggcaga taacaactgg cgtttagcac cgatagctgc cgataagaaa
     2761 ctggatatcc gtttcgaaac ttccccgtca gataaagccg cagcgtttat taaagagaaa
     2821 gggcagtatc cgatgaataa agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg
     2881 gatttgagta agtaaaacac ttctttttcg gcctataaat caaccgcatc cggcatttat
     2941 tggcggatgc gatgctgccg tatcttatcc accctacaag ccatgaaccg tagaccggat
     3001 aagcgcagcg catccggcag ttatgccgca cgttcatccc gcaccgccaa cacctcgggc
     3061 aaattcaact caatccagtc cgccagtgca gcaacctttt cgctcacctg ctcgcccagc
     3121 ggcgtgaggc tatattccac atgcggcggc accaccggat acgcgatacg gttaagaaaa
     3181 ccatcctgtt ctaacgcctg taacgactgc gcaagcatct tttcactcac cccaccgatt
     3241 ttgcgccgca ggtcgctaaa gcgatgagta ccttcgcgta gcgccaccag aatcaacacc
     3301 ccccaacggc tggtgacgtg tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga
     3361 ttaccctctt tcagttgttg tgacaggcta acctgactca tttcatactt acctttttgc
     3421 acgtacttac taaaagtaag tttaggtgtt agcgtattta aacacaagac aaaacgatgg
     3481 agacttccca tgatcgctat tactggtgcc actggccaac ttggtcacta tattattgaa
     3541 tccttgatga aaacggttcc tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa
     3601 gcccaggccc tggcagcaca aggcattacc gtgcgtcagg ctgactacag cgatgaagcc
     3661 gcactgacat ctgcacttca gggagtggaa aaactactgc tgatctcttc cagcgaagtg
     3721 ggtcaacgtg ccccgcagca tcgtaatgtt attaatgccg caaaggcggc tggcgtgaaa
     3781 tttatcgctt ataccagcct gctacatgca gatacctccc cgctcggcct cgccgatgag
     3841 cacatcgaga cggagaaaat gctggctgat tctggcatcg tttacaccct gctgcgcaac
     3901 ggctggtaca gcgaaaacta cctcgccagc gccccggcag cactggaaca cggcgtattt
     3961 atcggtgcgg ctggcgatgg caaaatcgcc tcagcaacgc gggcagatta tgcgacagct
     4021 gcggcacgcg tgattagcga agccggtcac gaaggcaagg tttacgaact ggcgggcgat
     4081 agtgcctgga cgttgacaca gttagcggca gagctgacca aacagagcgg caaacaggtt
     4141 acctatcaaa atctgagcga agccgatttc gccgcggcac tgaaaagcgt cggactgccc
     4201 gacggactgg cggatatgct ggcggattct gacgttggcg catcgaaagg tggtctgttt
     4261 gataacagca aaacgcttag caaattgatt ggctgcccaa cgacaacgtt agccgaaagc
     4321 gtaagccatc tttttaatgt taataactag ttaattaaag tggcatcctc ccgcatcctc
     4381 tctgataatg acgggatgcc gggagcaatc atgtctgctt cctgaacgtt tcttctgaca
     4441 gaccaatgga tgccagtaat gattagcggc gtgctgtacg ccctgttagc agggttgatg
     4501 tgggggctta tttttgtcgg gccgttgatc gtgccggaat acccggcgat gttgcagtcg
     4561 atggggcgtt atctggcgtt agggttaatt gcgctaccca ttgcctggct gggacgcgtg
     4621 cgtctgcgtc agttggcgcg tcgggactgg cttaccgcct tgatgctcac tatgatgggc
     4681 aacctcattt attacttctg ccttgccagt gccattcaac gtactggcgc gcctgtttcc
     4741 acgatgatta tcggcaccct gccggtggtg attcctgtct ttgccaatct gctttatagc
     4801 cagcgcgacg gcaaactcgc gtggggaaaa ctcgccccgg cactgatttg tattggcatc
     4861 ggcctggcga gtgtgaatat tgctgagtta aaccacggac tccccgattt tgactgggca
     4921 cgttatacct ctggcatcgt gctagcgtta gtttccgtgg tctgctgggc atggtatgcc
     4981 ctgcgcaacg cccgctggct gcgggaaaat cccgacaaac atccgatgat gtgggcgacg
     5041 gcgcaggcgc tggtcacgct gccggtttct ctcatcggct atctcgtcgc ctgttactgg
     5101 ctgaatatac aaacgccgga cttctcctta ccttttggcc cccgtccgct ggtgtttatt
     5161 agtctgatgg ttgcgatagc cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc
     5221 gccagccagc gattaccgac agtgattctc gggccgctga ttgttttcga aacgctggca
     5281 ggtttgctgt acaccttttt actccgccag caaatgccgc cgctaatgac gctgagcggt
     5341 atcgcgctgt tagtgattgg cgtggtcatc gcggtcagag caaaaccaga aaaaccttta
     5401 actgaatctg tctcagaaag ttgacatgct ggcagtgagt taaataagcc tctgctacgt
     5461 aagggttata gcttttgcct taaaggtgta tttaaaatac atcttatctt attaagaatg
     5521 aggtatcagc tatggcttat cgcgaccaac ctttaggtga actggcgctc tctatccctc
     5581 gcgcttcggc tctgtttcgt aaatatgata tggattactg ctgtggcggt aagcagacgc
     5641 tggcgcgcgc ggcggcacgt aaagaactgg atgttgaggt cattgaagct gaactggcaa
     5701 agcttgctga acaaccgatt gagaaagact ggcgtagcgc cccgctggca gaaatcattg
     5761 accatatcat cgtgcgctac cacgatcgtc accgcgagca gctgccggag ctgatcctgc
     5821 aagcgactaa agtcgagcgc gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa
     5881 aatacctgac catgctgcat gaagagcttt ccagccacat gatgaaagaa gagcagatcc
     5941 tcttcccgat gatcaaacaa ggcatgggca gccaggcaat ggggccaatc agcgtaatgg
     6001 aaagcgagca cgatgaagcg ggcgaactgc tggaagtgat caaacacact accaataacg
     6061 tcacaccgcc gccagaagcc tgcaccacct ggaaagcgat gtataacggc attaatgaac
     6121 tgattgatga cctgatggat cacatcagtc tggaaaacaa tgtactgttc ccacgcgccc
     6181 tagcgggtga gtgatctaaa agctggatgg cattgcgcca tccagcatga taatgcggtt
     6241 atttccgcag ttcagcagcc cgcttcttac caataaacag ccagcccaac cccagcgcga
     6301 taaaccacag cggggtaacc agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca
     6361 cgaccacgaa cacaaagaac gccatacata cccagcacat cagcttgccg agcggcatct
     6421 tgtagatcga cttttcatgc agatgaggac gctgtttgcg gtacaccagg taagagcaaa
     6481 ggataatcgt ccagacgaac ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg
     6541 cgccaatcac gctaggattc acatacaaca tcaccacgcc gccgagcaga cagatacacg
     6601 agaaggtcaa ccctttcgcg ggtactgcgc gcttagaaag tttagcgaac gctttcggcg
     6661 caacaccttc ctgcgccaga ccaaacagca tacggctggt agagaagaca ccgctgttag
     6721 cggaagacgc cgcagaggtc agcaccacaa agttgatcac gctggcagcc gcaggcaaac
     6781 ctaccaacac aaacagctca acgaacgggc ttttctccgg gactaccgaa ctccacggtg
     6841 tcacggacat aatcacaatc agcgcgaaga cgtagaacat aatgatacgg atcggaatgg
     6901 agttaatcgc gcgtggcagt gatttctccg gatctttggt ttccgcagct gttgtaccta
     6961 ccagctcaat ccccacgaaa gcgaaaaccg ctatctggaa tccggcaaag aagccactta
     7021 aacctttcgg gaaccagccg ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg
     7081 tcggtgactg aaagtgcatc gccaccatga ccaggccgac gacaatcagc gacacgatgg
     7141 cgacgatttt gatcatcgca aaccagaact ccatctcacc gaacattttc acggtggcaa
     7201 ggttgagcgt cagcagcagc actatcaccg ccagcgaggc gacccagtcg gagagatcgg
     7261 ggaaccagaa ctgagcataa gccgtgatcg ccaccacgtc tgccataccg gttacaaccc
     7321 agcagaacca gtaagtccag ccggtgaaat atcccgccca cggccccagt aaatcggaag
     7381 cgaagtcact aaaagatttg tattccagat tcgaaagcag caattccccc attgcccgca
     7441 tcacgaaaaa gagcataaaa ccaatgatca tataaacgaa aatgatcgac ggcccggcaa
     7501 ggctaatcgt tttgccggac cccataaaca acccagtacc aatggcacct ccaatggcaa
     7561 taagctgaac atgtcggttt gtgagattgc gccgtagcga ctgttcagcc ggagcctgat
     7621 catcggcaac gacttttacc tgatctacca tgttttttct tcctgtacct gtctgtgttg
     7681 ttcaggctct acggccttta gtcagtctat gatacgacga atctgtatcg ggactcatcg
     7741 atattaggta acaatacgcg ggatgaatac taagatttag atctaatgtt aattttatgt
     7801 ttaaagtgag tgtcatatca cggtgataac gcgaaacagc tcacaaaaat acacgcttac
     7861 ggggtctttg caacacaaaa tagcgccgtt tggcaaactg aagggtttat tgctgaatgc
     7921 ctgctcccct ctggttcgag gggaacagaa tgcgctgctt agaggatttc cagcagttcg
     7981 acttcaaaca ccagggtgct gaacggaggg atggatgcac ctgcgccgcg ctcgccatat
     8041 gccagttcct gcgggatagt cagttcccat ttagaaccta ccggcatcag agtcagtgct
     8101 tcaatccagc cagggatcac gccattaacc gggaattcag cgggttcacc acgagcaacg
     8161 gagctgtcaa acacggtgcc gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg
     8221 gtacgtgccg gaattgcgcc ttcaccctgg ttgatcacgc ggaattgcag gccagattcg
     8281 gtgctattca caccttcttt tttggcgttt tcttccaggt atttcacgcc ttcagcagcc
     8341 atcgcctgga aacgctgacg acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg
     8401 cgatgcacca catcaaccgg cacagccgga tgtttgcctt ccagcgcatc ggcaatacct
     8461 gcaaccagtg cttctggcag cagcccttcc aggccagatt cactcagttg ttgccctacc
     8521 tgcaaaccaa tgccgtagct tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc
     8581 gtatttcctt tcttagggta taaaagtagc gggcagcata tcagccacac ttctttgggt
     8641 aaaactttgt cgcaggaatg atgacaaatc ccggggaaaa agaaacaata gcaatatccc
     8701 gtatgaatat aataaaggcg ggtttaccac ggacatcata gcgttagctt attcataggc
     8761 tatgattgag gaacaagacg tggagcagga ggaaaccatg cccgggcgct ttgaactaaa
     8821 accaaccctg gagaaagtct ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc
     8881 gccgatgcat cgtcggggga tcattattgc cgccattgta ttggtggtcg gatttctgct
     8941 tccatctgat gatacgccca acgcgtcggt ggtgacgcgt gaagcgcagt tagatattca
     9001 gtcgcaatca caaccgccga cagaagaaca gccgcaaact acccaaacgc aaccattcca
     9061 gccagatagc ggaattgata atcaatggcg ctcttaccgt gtcgaacccg gtaaaacgat
     9121 ggcgcagctg ttccgcgacc acggactgcc cgctaccgat gtctatgcaa tggcgcaggt
     9181 agaaggcgcg ggtaagccgc tgagtaattt gcaaaatggg cagatggtga aaatccgcca
     9241 gaacgccagc ggcgtggtga cgggcttaac cattgatacg gggaataatc aacaggtcct
     9301 gtttacccgc cagccggatg gcagttttat tcgtgcgcgg tagatgaagg gtcaaccaaa
     9361 agccagctaa ggattgtctc tttgatgacc ttacgtctgg cttccagtgc ctggggggaa
     9421 accatactga tatgataaac taggccaagc gtatgctggt tgatcaaata atatccccca
     9481 agcgcagcaa tattaatatt cacctgcaat gggtcgatac ctggcttaaa aatattgtat
     9541 ttcttccctt catccaataa ggattccatt aactgcaagt gagcatggtt aatttctaag
     9601 agacgctgtg attttgcata atgcacacct ttactttgat tttcactatg cacaattttt
     9661 aaaaaccaag gattagcaat ataataatcc catgtaaaat caataatttt ctcaatggct
     9721 ttttgtggcg gaagatctga gaaatcaagg gccttttctt tttgtcgaat atcgttccag
     9781 gtatattcca gcacttcgat aaataaattt tctttattac caaagtggtg ataaaccgtc
     9841 tgtttactac accctgctgc ctttacaata ttatctactc tggccccttc ataaccatat
     9901 tcggcaaatt cattaaccgc acatagcagc aatttttcct tcaggctctg tacgttgctg
     9961 gtatcaatat acataacgct tccccacgac ctcttcgtgg tgactatcat aacctgattt
    10021 gatttcagct tcagaaaaaa gggcaaaaac ttgtactttc atcaggttat caaatgtcct
    10081 gtgatcgcca tcgcatatca accaactaga tggtttatct ttttatcctc cagtcgcaag
    10141 ctaatcatca ccaaggaaac aaaattcaaa aaagagagct attgtatgaa atccactcgt
    10201 cctgttgccg tgattaccgg agccactcgc ggtattggaa agggctgtgc gcaggagctt
    10261 gctcgtggcg ggtttaacct gctgattaac gatcgtcccg atgccgacag cgtagaaaaa
    10321 ctgcatatca cacaacaaga atgtttggcg gaaggcgtgg aagtgatttg cttccctgct
    10381 gatgtcggtg atctatctct gcatgaggag atgctcgacg cggcgcaaaa ccagtggggg
    10441 cggctggact gtctgctcaa taacgcgggg atttcagtga aaaaacgcgg tgacctcctc
    10501 gacctcgaac cagacagctt cgatcaaaac attgccatta acacccgcgc gccgttcttc
    10561 cttgctcagg cattcagtaa gcgcttactg gcacagccga aaccggaagc ggagctgccg
    10621 caccgctcga ttattttcgt cagttccatc aacgccatca tgctggcgat gaatcgcggc
    10681 gaatacacca ttgccaaaac cgccgtttct gccgctgctc gtctgtttgc tgctcgcctt
    10741 tgtaatgaac aaattggcgt ttatgaagtg cgccctggtc tcatcaaaac tgatatgact
    10801 attccggcaa cggcttatta cgacgaactg attgccaaag gtctggtgcc gtggggacgt
    10861 tggggttatc cggctgatat tgcttccacc gtccgcgcga tggcggaagg taaactgatt
    10921 tacacctgtg gtcaggcggt cgcaatcgac ggcggcttaa gtatgccgcg cttctgaggc
    10981 tgaatcatga gagatctgtt acaacgcccg gatttattca gtatcaatac cgcgacactg
    11041 ggctataaaa cgccgctacc cgccattatt gatgcctgcg ccgcacgcgg tattggcgca
    11101 attgccccct ggcgcaggga gttgcagggt gaagatctcc agcaaatcac ccgccagctt
    11161 gccgccagta acatgagcgt ttccggtttg tgccgtagca cttactacac cgcgccgaca
    11221 ctggcggaac gcaaactggc gattgatgat aaccgccggg cgctggacga cgcagcggtg
    11281 cttaacgccg cctgttatat gcaggtggtt ggcggtctgc caacgggcac taaagatcta
    11341 tatgaagcgc gagaacaagt gaaacaaggg attcgccagt tgctgccaca ttcgaaagat
    11401 gtcggcgtgc caattgcgct ggagccactg cacccgatga cagctgccga tcgctcttgc
    11461 ctgtgcacgt tacgtcaggc gctggactgg tgtgatgaac tcgatcccga tggtgagttt
    11521 ggcctcggcg tggcggtgga tgtttatcac gtctggtggg atccggatct cgccagtcag
    11581 atcctgcgtg ccggaaaacg catcctcgcg tttcatgttt ccgactggtt aatgccgacc
    11641 actgatctgg tcaatgaccg tggtatgccg ggagatggcg tcatcaatat tccgtcaatt
    11701 cgtcggctgg ttgaaaacgc cggatttaat ggtgctattg aactggaaat attctcgcct
    11761 tactggtggc agaaagatat taacag
//
LOCUS       DAZOZX010000123        11682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZOZX010000123 DAZOZX010000000
VERSION     DAZOZX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.123"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            47..610
                     /locus_tag="ACHUPT_003227"
                     /pseudo
     CDS             47..610
                     /locus_tag="ACHUPT_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHUPT_003228"
     CDS             complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHUPT_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0820676.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHUPT_003229"
     CDS             complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHUPT_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0820677.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHUPT_003230"
     CDS             complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHUPT_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0820678.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHUPT_003231"
     CDS             complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHUPT_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0820679.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHUPT_003232"
     CDS             2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHUPT_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR0820680.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHUPT_003233"
     CDS             complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHUPT_003233"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0820681.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHUPT_003234"
     CDS             complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHUPT_003234"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0820682.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHUPT_003235"
     CDS             complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHUPT_003235"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR0820683.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHUPT_003236"
     CDS             complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHUPT_003236"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0820684.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHUPT_003237"
     CDS             complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHUPT_003237"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0820685.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHUPT_003238"
     CDS             complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHUPT_003238"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR0820686.1"
                     /translation="MEILYNIFTVFFNQVMTNALLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHUPT_003239"
     CDS             7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHUPT_003239"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0820687.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHUPT_003240"
     CDS             8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHUPT_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0820688.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHUPT_003241"
     CDS             complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHUPT_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0820689.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHUPT_003242"
     CDS             10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHUPT_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0820690.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHUPT_003243"
     CDS             10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHUPT_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0820691.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11336..>11682)
                     /locus_tag="ACHUPT_003244"
     CDS             complement(11336..>11682)
                     /locus_tag="ACHUPT_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0820692.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT         2959 a         3087 c         2870 g         2766 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcagtaa caatgaatgg
       61 tgataattct tttggatgga accatgaagc tacattagat aatattcacg cccagaaata
      121 caataaagct tacgcgggtg cgaggttaac cgcagaactg aaatatttat tgcaagatgt
      181 cgaatccttc gaacccaaca gtaaatacac aatacacgag gttgttttgg gacctggtta
      241 tagtactcca gactataccg gccaaacgat aggatacgtt gtaacccttc ctgcccaaat
      301 gcctaattgc tgctcatctg aactaccaac aatcgatctt tatatcgacc aacttcacac
      361 tgttacaggc gtttctaatg ccctcggatt catcattgct gcattgctaa acgcgtattc
      421 tgatttaccc catgatttga aaattggtct acgttcctta tcaaattcag ccgcaatcta
      481 ctcgggttta ggtttcgagc gagtgccgca ggaccgagac ataaggagtg atcgtatgca
      541 tctaacgcca gcaaaccatc ctgacttatg gacacaagag aatggtgaat ggatctactt
      601 gcgaaattaa tgcctgacga aaaatagaaa gcaaaacgcc gaccaatggt cggcgttttt
      661 acgtctcgtt gaataacgaa ttattcagct actacgttta cgatcacttt cgcgaatact
      721 tcgctgtgaa cctggaagct cacttcgtgt tcgccagtgg tacgcagaac gccgttcggc
      781 agacgaactt cgctcttagc cacttcaacg ccagctgcag ttacagcgtc agcgatgtcg
      841 cgagtaccga tggaaccgaa cagtttacct tcgtcgccag ctttagacgc gatggtaaca
      901 gtttccagtg cattgatttt ctcagcgcga gcattagcag ctgccagaac ttcagccagt
      961 ttagcttcca gttcagcgcg acgtgcttcg aagaattcaa tgtttttctt ggtagctgga
     1021 acagctttac cctgcggtac caggaagtta cgagcatagc ccgctttaac gtttacctga
     1081 tcacccaggc tacccaggtt tgctacttta tcaagcagaa taacttgcat taccttatcc
     1141 tctcaaagtc gtattaatgg accgtgaccg attactgatg gcgatcagtg tacggcagca
     1201 gggacaggta gcgagcgcgt ttgatagcgc gagccagctg acgctggtat tttgcacggg
     1261 taccggtgat acggcttggg acaatcttac cgctttcggt gatgtagttt ttcagcgtag
     1321 cgatatcttt atagtcgatc tcttgaacgc cttccgcggt gaaacggcag aacttgcgac
     1381 gacggaaata acgtgccata tggctagtct ccagaatcta tcaattcaat ctgctcggca
     1441 tgcaaaacca ttttgctcag tccgttcttt gccttgtggc atgaaatgaa cccctgaacg
     1501 gttatgcgac tgccgaccgt tatactgtga gtaatggcct ggttttcgtg tccgctaaca
     1561 ataacgggca tttgacacca tgcctgccgg tgaaagccgg cttcctcctg cacagaacga
     1621 tgctcaagca cgaactggca gtgaggaatt cctgatggac tgacctttcg aaggggagtc
     1681 ctgcacacgg tgccggacaa caccagacgg ttggtcatca gaaattactc ttcagaatcc
     1741 ccagcttcag catcatcagc ggtttcgttt gcgaaatcat cgcgacgctc acggcgctcg
     1801 tctttcgctt taaccatcgg agatgcttcg gtaacagcgt gcttggtacg cataaccatg
     1861 ctgcggataa cggcatcgtt gaagcggaag gtagtttcca gctcatcgat cacttcctgc
     1921 ggagcttcaa cgttcatcag aacgtagtgt gctttgtgca gtttgttgat cgggtaagcc
     1981 agctgacggc ggccccagtc ttccagacgg tggatcttgc cttctgcacc agtgatggca
     2041 gcagtgtagc gctcgatcat gcccggaacc tgttcgctct ggtcaggatg gaccataaaa
     2101 acgatttcgt aatgacgcat cgaattgctc cttacggatt attcagcctc ctgtctgggt
     2161 cagccgaatc ccggggaggc aaggaacgtg ttaaaggtcg gctgaaaaat gacgcgttat
     2221 agtactggca taccccgcta aactcaaggg cggagcagaa ataaatctcc cctttcgcaa
     2281 ataacctcta aaccgctgat ttgaagttca tcacattccg tcacagtgtt ttttgaacaa
     2341 ctgcatcaga aatggtcacc atcaacttct ttatcattga ttaaaattaa atcagaagat
     2401 catcacagag gagagcacac aaagtcagcc gtcgaggagg tatctatgtt cagtcgagtt
     2461 ttagcccttc tggctgtgct tttgctaagt gcaaatacat gggcagccat tgaaattaat
     2521 aaccaccagg ccagaaatat ggacgatgtg caaagcttag gcgtgattta tatcaatcat
     2581 aatttcgcca ctgaaagcga agcacgtcag gcattaaatg aagaaacaga tgcgcaaggc
     2641 gcaacgtact accacgtaat tctgatgcgg gaaccgggga gtaacggcaa tatgcacgcc
     2701 agcgcggata tttatcgcta gcaccaggta taaccaacga aacattgcca tagtttgctt
     2761 tgcccccttc gcagggggct ttttttgaaa aacggctgcg gaattagacc agttatctcc
     2821 cgaggaagga aatttccgca gcgcgtgttt tacttctgcc cgtaataagc gttaggaccg
     2881 tgcttacgca tgaagtgttt attcatcagg tagctgtcga tatgattgag ttgtggatta
     2941 atactgcgcg caatccacgc cattttcgcc acttcttcca tcactaccgc gttatgcacc
     3001 gcgtcgtggg catctttccc ccaggcgaac ggtccgtgct gatacaccac aattcccggc
     3061 gtatgcagcg gctcggcgtt gcccagcgtt tcgataatca ctttgccagt gttcagttca
     3121 tactcaccct gcacctcttc ttcgcttaat ccacgcgtac acgggatatc accaaagaag
     3181 tagtcagcgt gcgtggtgcc taatgccggg atcgccagcc ccgcctgcgc ccatgcggtg
     3241 gcatgagtgg agtgggtatg gacaatgccg ccaagcgacg ggtaacgacg gtagagttcg
     3301 agatgcgtcg cagtgtcgga agatgggcga tactcccctt ccaccacctt gccgctcata
     3361 tcgaccacca ccatatcgtc tgctttcatg gtttcgtagg caacgccgct gggcttgatc
     3421 accaccagcc cgcgttcgcg gtcgatagcg ctgacgttgc cccaagtaaa ggtcaccagc
     3481 ccatagcgcg gcagatccat gttggcttca aacacctgct gttttagctt ttgcattatg
     3541 ccgcctccac catgcctgct ttggccatgc gcgctttcac ccaatcacac gctttcacca
     3601 cttctgccgc cgggtcttcc gccgtttcgc tccacatctc aatcaggtac ggcccgcaat
     3661 agccactctg tttgagcgtt tcgaagcaac gttcgaaatc caccacacct tcgccaaatg
     3721 gcacgttttt gaagacgccc ggtttggtgt ctttcacatg caccgcgacg atatgcccga
     3781 ttccggcctg caactccatc tgcacatcgt tgtcccacgc cgacaggttg ccgatatccg
     3841 ggtagagctg gaaccacggg ttgttgagat agtgcgcgta acccagcgcc ttgctgatgg
     3901 agttcatcaa cggataatcc atgatctcca tcgccagcgt cacctgcgcg cggctcgcca
     3961 tctcaacgct ctctttcagg ccgtcacgga aacgacgacg cgtttcgtta ttggcttcct
     4021 gatagtaaac gtcatagccc gccagctgga tcacgcgaat gccaacgtcc tgggcgaact
     4081 ggatggcttt acgcataatc tccagcccct gcgcccgcac cgcgtcatct tcgctgccca
     4141 acgggaaacg acgatgggca gaaaggcaca tggacggcac acgcacgccg gtttcaacaa
     4201 tagcattcac cagcgccaga cgctgttcgc ggctccagtc aaggcgcgac aggcgctcgt
     4261 tagtttcatc taccgacatc tcgacaaaat cgaagcccaa cgtttttgcc agtcgcaggc
     4321 gttccagcca gcactccccg gcggggagcg ctttttcata gatgccaagc gggatttgtt
     4381 tggacaacat atccgctcct tagccccaca gttcggcgat ggaacgtttg aactgacgcg
     4441 cggcttcaac cggagaagcg gcatcacgga tgctgcgacc ggcgataaac acatgaattg
     4501 gaatgccttt gaacagcggc agatcttcca gcgccaggcc accggtgacg gtgactttga
     4561 agcccatatc ggaaagacgt ttgatcgcag tgatatcggc ttcaccccac gccacgccag
     4621 cggcctgcgc gtcacggctg cggtgataaa ccacctgctg aatacctgcg tcgcgccact
     4681 gttgcgcctg ttcccaggtc cagtaaccgg tcagttcgat ctgcacgtcg ccgttaaact
     4741 cttttgctac gtccagcgca cctttggcgg tgttgatatc cgcacaacaa atcacggtca
     4801 cccagtcagc gttggcttcg aagcacatac gagaaagaat tttgcccgca tcggcaattt
     4861 tggcgtctgc cagcacgatt ttgtgcgggt agagcgcttt cagatcgcga accgcacgca
     4921 cgccttcgcc cacgcacaga atggtaccca cttcgataat gtcgacttct tcggcaatca
     4981 ggcgagtggt ttcgtaggcg ctatccatag tctggttgtc cagcgcgact tgcaacatcg
     5041 gtaatgacat cttcaattcc ttcttaagct gccgcgttgg tgcggtcaat gagatccagt
     5101 acttcctgct cggtacggca ggcacgtaaa cggtcgaaat tttcttcatc ttcaaacagg
     5161 ttgacgatct gcatgatgcc cacttcctgg tgcgtgttgg catcgaccgc cgccatggtg
     5221 atgaggatat ccaccgggtc gttatcttcg tggttgaact ccagcggctt tttcagcgtc
     5281 accagcgcaa aaccggtttt cttaacgccc tcttccgggc gaccgtgcgg cattgccagg
     5341 cctggtgcga gcacaaagta cggaccgtgc tgttcaaccg catccagaat cgcctggtag
     5401 taacgcggct cgacaacatc cgccgccacc agcaggtcaa caccgatttt tactgcgtcc
     5461 tgccatgtct cagcttcagc ctgcaggcgg atggatttat tctccgccag cgaatcatgt
     5521 aatttcatgg cgcgtcctta cttcacatcc tgcgggaaat gcgctttgat cacttccagc
     5581 agtttcgggc caaagtcggc aggagagagc atgttgcgca cgccaaccac gtatttgttg
     5641 ccggtcacgg tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc cgcgccgctc
     5701 aactcgcttt tgtattcgcc aaccgcgcag ctatttaccg tgtggtcaat gtttgattgg
     5761 gttaaaaact ggtccacttt catcttcatg atcatggaac tgccttgtcc gttgccacac
     5821 acagccagaa tacgtacggt cataatcaaa actccttatt aagcagactg ttctgccaat
     5881 tgtttttctg catcttcttc tgcgcgcagt gcgcggccag cgaagaacat ataagccagt
     5941 gcaattacaa tgatgacggc cataaaggcg ataccgatgg agaagaagcc ctgcatcatc
     6001 ggcggtgcca gaatcgacca gtccgccatc cccatccagg cactcatacc ggtaagtttc
     6061 accacccaga cgcaaccaaa gatttcgatc atccccatca ccagacaaat cttcagcgcc
     6121 gcacgccagc cgccgaagtg gttagcgaac acgccgatgg tggcgttgga gaagaacatc
     6181 gggataaagc ctgggataat caggatcgag gaaccgcagg cgaccaggat gccaaccgca
     6241 atcagctgac cgatggtgcc ccacataaag ccccagacca cggcgttcgg agcgaagcta
     6301 taaatagccg cacagtcaat cgccagaacc gcaccaggga tcaggcgctg ggagataccg
     6361 ttaaacgctt cagagagttc cgccacaaac atgcgcacac cctgagtgat gatgaagatc
     6421 gccaccgcga aggaaaaacc ggtttgcagg atatagaccg tccagtgcac tttgcctgcc
     6481 atcgcctgca cggtgtcgat accgaaggag agcagaatgg caccaaagaa gatggtcatg
     6541 acaatcgccg tggagacgat gttgtcgtgg aaaatattca gccagcctgg cagtttgagg
     6601 tcttcaacgc tttcctcttt tttgccgagg aacggcgcaa ctttataggc aattaatgat
     6661 gcaaactgtt gctggtgacc gatggagaaa ccgcagccgt ccgtcacttc ctgagtcggc
     6721 ttgtacatca tgttggaggt aatgccccag tagagcgaaa ccagaatcgc cgtgcagata
     6781 atggttgtcc acatggagta gccgaaaata aacaacgtaa cggcaatcag ccccgcctgc
     6841 tggaacatga tatgaccagt cagcatgatg gtacgaatac cggtaatgcg acgcagcagc
     6901 acgtaacaga tgttcagcgc cagcgctaac aacaccgcgt aacctaccca gctataggca
     6961 tcgcccatgc ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat tgcgccgtta
     7021 atgccgtaga cttcggacat tttcgccacc accggtttga aggtgctggt gaggatgccg
     7081 gaccctgcct gcaacaacat gaaaccaatt atggttttaa tcgtgccttt aataataacg
     7141 ctgacacttt tgcgcagtag gatgtagccc agacaggtca caatacccag caacaacagg
     7201 gcattggtca tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat ctccataacg
     7261 atctcctgaa gaagaggtaa ccgggtattc cgcacacccg gaatgttgtg cactcactct
     7321 aattttcaaa agtaatcaca acaagattat ttatgattaa atgtgacgcg acccgcaaat
     7381 aattccacat ggatagagtg ggattcacac aacgaaacaa ttatttattt aaattcattt
     7441 agttaattta actacacatt ccattaaatc tttccccact caaaatcaac aacaacttcc
     7501 ctggcaaaaa gtcaaaaaat ggtcattacc ttcctgaagg aatagtgtca ggattaatca
     7561 aaaccaatca catgttgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag
     7621 tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc
     7681 tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag
     7741 gctgcaccgg gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt
     7801 gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc
     7861 gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac caccccgttt gttcttgatc
     7921 cgtttgcgat tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg
     7981 acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca ttcattggac
     8041 cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg
     8101 tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt gacgctttcg
     8161 accgtactgc actgatcacc ctgcctgccg atcaaaaagc ggctggcgta ctgccagatg
     8221 gcatggacga tcgcgcagta aactacctgt tcaaaacccc tggcggttcc ctatatcaca
     8281 gtggcgattc ccactactct aactattatg cgaagcacgg taacgaacat cagatcgatg
     8341 tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg accagcgccg
     8401 atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc caccacgaca
     8461 tctggtcaaa cttccaggcc gatccgcaag agatccgcgt gctgtgggag atcaaaaaag
     8521 atcgtctgaa gtatggcttc aagccgttta tctggcaggt tggcggcaaa tttacctggc
     8581 cgctggataa agacaacttc gagtaccact atccgcgcgg tttcgatgat tgcttcacta
     8641 ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg
     8701 gagaagttcg cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa
     8761 tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat
     8821 gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc
     8881 tgccactgcg cgacgcgata tcaataaact tggcgaaagc ggcaaactga aaaaggtgcg
     8941 caatggcgca gaagctatta cccaacagcg cccgcgctgg acgccgatga atctgcatca
     9001 ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc tggttaatcc
     9061 gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg
     9121 tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca
     9181 agaacatgac agcgtgatca ttatgggtgg acagtacaac aaaagtcagt ccatcacttt
     9241 aagcccgcag ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggaaa
     9301 agggctgacc gcagaagggt tgtataaaac cgatatgctg acagcaatgg cagagcagaa
     9361 gatgctgagc gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg
     9421 cgcgggaatg ctttttagcc gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc
     9481 taacccggaa atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag
     9541 atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac
     9601 gcctggctgc catgaacagg ttagcagttt atcccgtccc gtctcgctta aggcctgctg
     9661 caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc acagcagtag
     9721 aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac
     9781 aattttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga
     9841 acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt tcaccgtggg
     9901 gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac cgactgccag
     9961 acggtcatca agcagccagt tttcttcggc tattgccgca cgtaaagtag tgaattcctg
    10021 catactttgt agcaagattt gccagaattg atttaaccgc cgcgcttcgt cgccactaaa
    10081 acggctaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc
    10141 aacggcaaaa tagctataca ccaggctgga tgaagtaaag ccgtgataaa aaattacgca
    10201 cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag
    10261 ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa
    10321 tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct
    10381 ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa
    10441 agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg ttatttaccg
    10501 gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc
    10561 acagccagca ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc agacgcaagg
    10621 gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg acgcattagc
    10681 agaaatcaga gcaaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga
    10741 cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag
    10801 acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat
    10861 aaattaccgg ctggaagctt gggcgtttat taccgcgatg gaatgccctg caacatgtgg
    10921 ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag
    10981 tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga
    11041 cgggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg
    11101 tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta
    11161 ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg tatgcatgag
    11221 tgtcatctac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctacaa
    11281 ttttcacata tttcatttag ttaatcgaaa ccagcgtcgc atcagtcgta tacgcttaca
    11341 cacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta
    11401 cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc ggagaagcat
    11461 atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcccca
    11521 gttcttcagc tggcttacgc agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc
    11581 gatcctgccc tttcacttcc acaaatgctt ccgacaataa gtcgtatacg cccgcttgct
    11641 tattgagaac gcgcaacaca tccaggcaca taatattgcc gg
//
LOCUS       DAZOZX010000124        11519 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZOZX010000124 DAZOZX010000000
VERSION     DAZOZX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.124"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1046)
                     /locus_tag="ACHUPT_003245"
     CDS             complement(<1..1046)
                     /locus_tag="ACHUPT_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0820693.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1626
                     /locus_tag="ACHUPT_003246"
                     /pseudo
     CDS             1537..1626
                     /locus_tag="ACHUPT_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            1928..2923
                     /gene="pgl"
                     /locus_tag="ACHUPT_003247"
     CDS             1928..2923
                     /gene="pgl"
                     /locus_tag="ACHUPT_003247"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0820694.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2964..3917)
                     /locus_tag="ACHUPT_003248"
                     /pseudo
     CDS             complement(2964..3917)
                     /locus_tag="ACHUPT_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4101..5153
                     /locus_tag="ACHUPT_003249"
     CDS             4101..5153
                     /locus_tag="ACHUPT_003249"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR0820695.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            5229..6662
                     /locus_tag="ACHUPT_003250"
     CDS             5229..6662
                     /locus_tag="ACHUPT_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR0820696.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6845..9025
                     /locus_tag="ACHUPT_003251"
     CDS             6845..9025
                     /locus_tag="ACHUPT_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0820697.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(9166..10449)
                     /locus_tag="ACHUPT_003252"
     CDS             complement(9166..10449)
                     /locus_tag="ACHUPT_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0820698.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(10584..>11519)
                     /locus_tag="ACHUPT_003253"
     CDS             complement(10584..>11519)
                     /locus_tag="ACHUPT_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0820699.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2874 a         2584 c         2858 g         3203 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg tagcgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaatg
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tagtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaggac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tcaaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga ttgaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt ttatttgtgc cggaagtgag
     1621 ttgattgttg cgcgaatggg aagttggcca gaagttgtta tggcatagtg tttacggtcg
     1681 ttgatacagg taaacaaaag cggggagagt catactccct gaatagtcgc gaaaatatct
     1741 gtggcattgt caaattcgtg tcggtgacta atattttagt aatatgccat tcgaaatgga
     1801 gtgctaactt attgatttaa ttgatgtgcg atattgtatt ttatcgtgtt ttgttgtctg
     1861 ttttttatcc agtaagtgtt gttatatgta tgaaagataa gggatttatc atttttgagg
     1921 tgtgttcatg aagcaaacag tttatatcgc cagccctgag agccagcaaa ttcacgtctg
     1981 gaacctgaat catgaaggcg cactgacgct gacacaggtt gtcgatgtgc cggggcaggt
     2041 gcagccgatg gtggtcagcc cggacaaacg ttatctctat gttggtgttc gccctgagtt
     2101 tcgcgtcctg gcgtatcgta ttgccccgga cgatggcgca ctgacctttg ccgcagagtc
     2161 tgtgctgccg ggtagtccga cgcatatttc caccgatcac caggggcagt ttgtctttgt
     2221 aggttcttac aatgcgggta acgtgagcgt aacgcgtctg gaagatggcc tgccagtggg
     2281 cgtcgtcgat gtagtcgagg ggctggacgg ttgccattcc gccaatatct caccggacaa
     2341 ccgtacgctg tgggttccgg cattaaagca ggatcgcatt tgcctgttta cggtcagcga
     2401 tgatggtcat ctggtggcgc aggaccctgc ggaagtgact accgttgaag gggccggccc
     2461 gcgtcatatg gtattccatc caaacgaaca atatgcgtat tgcgtcaatg agttaaacag
     2521 ctcagtggat gtctgggaac tgaaagatcc gcacggcaat atcgaatgtg tccagacgct
     2581 ggatatgatg ccggaaaact tctccgacac ccgttgggcg gctgatattc atatcacccc
     2641 ggatggtcgc catttatacg cctgcgaccg taccgccagt ctgattaccg ttttcagcgt
     2701 ttcggaagat ggcagcgtgt tgagtaaaga aggcttccag ccaacggaaa cccagccgcg
     2761 cggcttcaat gttgattaca gcggcaagta tctgattgcc gctgggcaaa aatctcacca
     2821 catctcggta tacgaaattg ttggcgagca ggggctactg catgaaaaag gccgctatgc
     2881 ggtcgggcag ggaccaatgt gggtggtggt taacgcacac taaccgctga tttaaccggc
     2941 gcagtctctc ctgcgccggt gtattaactt atctcctgta acgcatgtct ctggcgttcg
     3001 acgatattgg tccacaaatt gtctttatcg tcagtccaca aattaatcag caaggaaaaa
     3061 aagcgttcag ctgccggaga aagcacggca tctttgcggc gaataatccc caatgtccga
     3121 cgtataacgg gttccaccag tgggatacca ataatcgaag aatagggcgc atggggcatt
     3181 gccagaccag gaagcgccga aatccccagt cctgcctcca ctagtcctaa tgacgtcgaa
     3241 agatgacgca cttcgtaaaa ccagtccagc ttccagggct tgtcggctag ctgttgttct
     3301 atcaacaatc ggttgccgct ggaggaacgt acgccaatca ttttgtaacc cactaactcc
     3361 tgccattcta caagctactt tttggccaat ggatggtcac gccgacaggc cagcacgaat
     3421 ggctcgttga ccagtggggt aaaatcaatg gatgaatttg tgacgttgtt catgtttatg
     3481 ccaaagtctg attcgttaca cagcaccgat tccatgcaat tattcgtgcc ttgttccaga
     3541 atccgcactt taatattggg atacagctca ttaaatttac cgattgccag cggtaaaaaa
     3601 taaaatactg cggtcggaat acacgctaat gtcaccatac cacgatgata agcattcata
     3661 tcacgaatat taaaaagcgt ttcatcgaat ttctttatta attcccgcgc ctccgggagc
     3721 aacctttttc ctgcttttgt taacgtaacc ttgcgagttg tacgctcaaa tagttgaata
     3781 tgtaaatctt cctccatctt ttttattctt cgcgttaatg caggttgcgt aatattgagt
     3841 aatttagcgg cattgttaaa tgaactggac tctgccagta tgacaaatgc cttcatactt
     3901 gataattcat gcttcattat tactccggaa aatggaagcg acgattttgg ggggctggtc
     3961 gttaaaaatt ttaactgcat ttagccaact taaattaatg aaaaaatgtt attaatcgtt
     4021 gagctaaagt cattagagat gctttgtcct taatgtaacc atatcgcaat aagttatgtt
     4081 tttaaattga gggcattatt atgaaaaaaa taccctgcgt gatgatgcga ggtggaacct
     4141 cgaggggcgc gttcctgtta gcggaacatt tacccgaaga tcaaacgcag cgcgataaaa
     4201 tattgatggc aattatgggt tccggtaacg atctggaaat tgacggtatt ggcggcggta
     4261 atccactgac cagtaaagtc gccattatta gccgttccag cgatccgcgt gctgatgtcg
     4321 attatctgtt tgctcaagtt atcgtccatg agcaacgtgt cgataccacg cctaactgcg
     4381 gcaatatgct gtctggcgta ggggcattcg ccattgaaaa tggtttgatt gcagcgactt
     4441 cgccagttac ccgtgtacgt atccgcaacg tcaatacggg tacgttcatc gaagctgatg
     4501 tgcaaacgcc aaatggtgtt gtcgagtacg agggtagcgc cagaattgac ggcgtaccgg
     4561 gtactgccgc accggttgcg ctcactttcc tgaatgccgc tggaacgaaa actggaaaag
     4621 ttttcccgac tgataatcag attgattatt ttgacgatgt cccggtgacc tgtatcgata
     4681 tggcgatgcc agtcgtcatt attccggctg aatatctggg aaaaacaggt tatgaattac
     4741 cggcggaact ggatgccgac aaagcattat tagcccgcat tgaatctatc cggctacaag
     4801 cgggtaaagc aatgggctta ggtgatgtca gtaatatggt tatccctaaa cctgtgctta
     4861 tttctccagc gcagaaaggc ggggcaatta atgtgcgtta ttttatgccg cattcttgcc
     4921 atcgcgcgct ggcgataacc ggtgctattg ctatttccag tagttgtgca ttggaaggca
     4981 ccgtcacccg acaaatcgtc ccttctgtag gatatggcaa tatcaatatt gaacacccca
     5041 gtggtgcgct cgacgttcat ttaagtaatg aaggtcagga tgccacgacg ttacgcgcat
     5101 ctgttattcg gacaaccaga aaaatatttt ccggtgaagt ttatcttccc tgaaaaaatt
     5161 cgttgtcagg ataaggacaa tcaataaagg acttctgtat gagtcataca gaaagaacag
     5221 gattttaaat gaataagaaa tcgttatgga agctaattct gatattagcg atcccatgta
     5281 ttattggttt tatgccagct ccggcaggat taagcgaact ggcgtgggtg ctttttggta
     5341 tttacctggc ggccattgtg ggtctggtta tcaagccttt cccggaacct gtcgtactgt
     5401 taattgccgt tgctgcctca atggtggtgg tcggtaactt atccgacggt gcgtttaaga
     5461 ccaccgccgt attaagcggt tactcttcag gtactacctg gctggtgttc tcggcgttta
     5521 ccttaagcgc cgcatttgtg accaccggtt taggtaaact tattgcctat ctgctgattg
     5581 gtaaaatcgg taacaccatg ctgggtctgg gttacgttac ggtattcctc gatctggtac
     5641 tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg cattgtgtta ccgatcatca
     5701 acagcgtggc ggtggctttg gggtccgaac cggaaaaaag tccgcgtcgt gtcggacatt
     5761 acctgatgat gtccatttac atggtcacca aaaccaccag ctatatgttc tttaccgcaa
     5821 tggcggggaa cattctggcg ctgaaaatga tcaacgacat tctgcacctg caaattagct
     5881 ggggtggatg ggcgctggcc gccggattgc cgggcatcat tatgctgctg gtcaccccgc
     5941 tggtaattta caccatgtat ccaccagaaa ttaaaaaggt ggataacaaa accatcgcta
     6001 aagcgggcct tgccgaactg ggaccgatga aaatccgcga aaaaatgctg ctcggtgtct
     6061 ttgtgctggc gctactgggc tggattttca gtaagtctct gggggttgat gaatccactg
     6121 tggcaatcgt tgttatggcg accatgctgc tgctgggtat cgttacctgg gaagacgtgg
     6181 ttaaaaataa aggcggctgg aataccttaa tctggtacgg cggtattatc ggcttaagct
     6241 ccttattatc gaaagttaaa tttttcgaat ggttagctga agtctttaaa aataacctgg
     6301 catttgatgg tcacggtaac gttgctttct tcgttattat tttcctcagc atcatcgtgc
     6361 gttatttctt cgcttccggt agtgcctata tcgttgctat gttacctgta tttgccatgc
     6421 tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct ggcactgttg ttctccaact
     6481 cctatggcgg catggttact cactatggcg gcgcggcagg tccggtcatc tttggcgtgg
     6541 gttacaacga tattaaatcc tggtggttgg tcggtgcggt actgacgata ttaaccttct
     6601 tggtgcatat caccctcggc gtgtggtggt ggaatatgct gatcggctgg aacatgctgt
     6661 aaatatactc gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc accccagtca
     6721 cttacttatg taagctcctg gggattcact cacttgtcgc cttcctgcaa ctcgaattat
     6781 ttagagtata tccatttatt atctttctgc gcacttcacg gtgcgcagat atctggagca
     6841 tttgatgatc aagttatctg aaaaaggcgt gtttctcgcc agtaataacg aaataattgc
     6901 cgaagaacat ttcaccggcg aaattaaaaa agaagaagca aaaaaaggca ctattgcctg
     6961 gtctattctc tcttctcata atacgtccgg aaatatggat aaacttaaaa ttaagtttga
     7021 ttcattagcc tctcacgata ttacctttgt tggtattgta cagaccgcta aagcgtccgg
     7081 aatggaacgt ttcccgctgc cgtatgtgct gaccaactgc cataactcac tctgcgccgt
     7141 cggcggcacc attaacggtg atgaccatgt ttttggttta tcggcagctc agcgttatgg
     7201 cggtattttt gtgcctccgc atattgcggt catccatcaa tatatgcgtg agatgatggc
     7261 tggcgggggc aaaatgatcc tcgggtcaga cagccacacc cgttacggtg cattagggac
     7321 aatggcagtc ggtgagggtg gcggtgagtt ggtaaaacag ctgcttaatg acacctggga
     7381 tatcgactat ccgggcgtgg ttgcggtgca tctgaccgga aaacctgcgc cgtatgtgga
     7441 gccacaggat gtggcgctgg ctatcattgg cgcggtgttc aaaaacggtt acgtcaaaaa
     7501 caaagtcatg gagttcgttg gaccgggcgt tagcgcgctc tctaccgatt tccgtaacag
     7561 cgttgacgtg atgaccacgg aaaccacctg tttaagctcc gtctggcaaa ctgatgaaga
     7621 agtgcataac tggctggcgc tgcacggtcg cggtcaggat tactgccagc ttaaccctca
     7681 accgatggcg tactacgatg gctgcatcag cgttgattta agcgccatca aaccaatgat
     7741 tgcgctgccg ttccacccga gcaacgtgta tgaaatcgac acactgaacc agaacctgac
     7801 cgacattctg cgtgagattg aaattgagtc cgaacgcgtg gcgcacggta aagccaaact
     7861 ctcgctgctg gataaagtgg aaaatggtcg cctgaaagtg cagcagggga ttatcgcggg
     7921 ctgttctggc ggtaactacg aaaacgtcat cgcggcggcg aatgcactgc gcggtcaatc
     7981 ctgtggcaat gacaccttct cgctggcggt ttacccgtca tcacagccgg tgtttatgga
     8041 tctcgccaaa aaaggtgtgg tagcagattt gattggcgca ggcgcaatca tcagaaccgc
     8101 attctgtggc ccatgctttg gcgcgggcga tacgccaatc aacaacggtt tgagtattcg
     8161 ccacaccacg cgtaacttcc cgaaccgcga aggctctaag ccagccaatg ggcagatgtc
     8221 agcggtggcg ttgatggacg ctcgttctat cgctgcgact gcggcaaacg gtggctattt
     8281 aacctctgcc agcgaacttg attgctggga caacgtgccg gagtacgcct tcgatgtaac
     8341 gccgtataaa aaccgtgttt atcagggctt tgtgaaaggg gcaactcagc aaccgctgat
     8401 ttacggaccg aacattaaag actggccgga attgggtgcg ctgactgaca atatcgtcct
     8461 gaaagtgtgc tcgaagatcc tcgacgaagt caccaccacc gacgaactga ttccttccgg
     8521 tgaaacctct tcttatcgtt caaatccgat tggtctggcg gggaatgtca gcgagctgac
     8581 agaggtgttt gcgcgcatta agcagattgc tggtcaggag catattgatc cgctgcaaac
     8641 tgaaattggt agcatggtct atgcggtgaa accaggcgat ggttctgcgc gtgaacaggc
     8701 ggcgagctgc cagcgtgtga ttggcggtct ggcgaatatt gccgaggagt acgcgactaa
     8761 acgctaccgt tctaacgtca tcaactgggg gatgttaccg ctgcagatgg cggaagtgcc
     8821 aacctttgaa gtgggggatt acatttacat ccctggcatt aaagcggcgc tggataatcc
     8881 gggtacgacg tttaaaggtt atgtgatcca tgaagatgcg ccggtaacgg aaattacgct
     8941 ttacatggaa agcctgacgg ctgaagagcg cgagattatc aaggcgggta gtttgattaa
     9001 cttcaataaa aaccgtcaga tgtaaaaagc gccatgtgaa tgtaggtcgc attcggcact
     9061 tattgtcgga tgcgatgctg gcgcatctta tccgacctac aaatcgcatc gaaccgtagg
     9121 ccggataagg cgtttacgcc gcatccggca aatagttaat agctcttact tcttctcctc
     9181 tgcaaccact ttgctgccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt
     9241 gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa
     9301 cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac cttcgttgat
     9361 ggcgctatca cggatcacca cctgaccgtt ggtattagca tcaacatcca gcgagcggcc
     9421 cagttgcgcc acgccatcac cggaagcatt gaaacggctg tttacggcga ggaaaccgta
     9481 atagatgtta gacagcgtag ccggtgcaaa cacatatgct tcttgctggg tacgtgagtt
     9541 caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac
     9601 atccccttca atatagctgt tggtcaccag cgtacgcggc tgacggttgg tttccagacg
     9661 gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt
     9721 gttaatctgt acttggtcac cgtcagtacg cagtgctacc gccggatggt tacctgcatc
     9781 tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcagtc cattgttttg
     9841 tgaccagaag accgcagaac acagaacacc gatactgtcg ctgcgtttgc tctggcagct
     9901 atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg
     9961 acgccagtcg gcagggctca tgcccccatc aagggaaagc ccaatcttca catcaatcgg
    10021 tttttcacct gtaccgtaca gagtgattcc acctggagca gcagggacat acaccgttcc
    10081 ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtgcgct tgataattgc
    10141 cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtacccg ccgggccgac
    10201 aacaaagtca ggttgcgcag gcagggttat cggggacgga ttccacgctg cagcacctgg
    10261 tgtcagggat gcaaaatagt gttgcgcatc gaaattcagc gcttcttttg ccgacagaat
    10321 cgggcgagaa gaggtaccag gcgcagtttg atcagaagga cgttgatcgg gtggtgttga
    10381 gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa
    10441 tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt
    10501 tttatactaa gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt
    10561 cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc cctgcctctg
    10621 tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg ttgagcaaac
    10681 ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc gtgaaaggta
    10741 ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa ataccttgat
    10801 actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc tccgccaaga
    10861 atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc aacatgcaat
    10921 gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag atatccatct
    10981 acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt aacaacagcc
    11041 agttccattg caagtctgag ccaacatggt gatgattctg ctgcttgata aattttcagg
    11101 tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg agtggcagcg
    11161 accgggtttg ttgttatatg gccttcagct attgcctctc ggaatgcatc gctcagtgtt
    11221 gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt gagcattgcc
    11281 gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct ccttattgct
    11341 ttaattttgc tcatgtaatt tatgagtgtc ttctgcttga ttcctctgct ggcgaggatt
    11401 ttttcgtagc gatcaagcca tgaatgtaac gtaacagaat tatcactgtt gattctcgct
    11461 gtcagaggct tgtgtttgtg tcctgaaaat aactcaatgt tggcctgtat agcttcagt
//
LOCUS       DAZOZX010000125        11393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZOZX010000125 DAZOZX010000000
VERSION     DAZOZX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.125"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            80..1221
                     /gene="yghO"
                     /locus_tag="ACHUPT_003254"
                     /pseudo
     CDS             80..1221
                     /gene="yghO"
                     /locus_tag="ACHUPT_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1345..2115)
                     /gene="glcC"
                     /locus_tag="ACHUPT_003255"
     CDS             complement(1345..2115)
                     /gene="glcC"
                     /locus_tag="ACHUPT_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR0820700.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2366..3865
                     /gene="glcD"
                     /locus_tag="ACHUPT_003256"
                     /pseudo
     CDS             2366..3865
                     /gene="glcD"
                     /locus_tag="ACHUPT_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3865..4917
                     /gene="glcE"
                     /locus_tag="ACHUPT_003257"
     CDS             3865..4917
                     /gene="glcE"
                     /locus_tag="ACHUPT_003257"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR0820701.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4928..6147
                     /gene="glcF"
                     /locus_tag="ACHUPT_003258"
                     /pseudo
     CDS             4928..6147
                     /gene="glcF"
                     /locus_tag="ACHUPT_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6152..6556
                     /locus_tag="ACHUPT_003259"
     CDS             6152..6556
                     /locus_tag="ACHUPT_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR0820702.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6578..8749
                     /gene="glcB"
                     /locus_tag="ACHUPT_003260"
     CDS             6578..8749
                     /gene="glcB"
                     /locus_tag="ACHUPT_003260"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR0820703.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFFRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9104..10690
                     /locus_tag="ACHUPT_003261"
     CDS             9104..10690
                     /locus_tag="ACHUPT_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR0820704.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2698 a         2793 c         3147 g         2755 t
ORIGIN      
        1 attgagttga ttattaagat gccgctcgtt gctgttgcaa cctgtttttc accattaatt
       61 atttgttatc cacagagata tggaatgcga tctgctgatg attaaaattg aaaaagtaat
      121 taataaaaat gaccttaagg catttatcgc ttttccgtca tcactttatc ccgacgatcc
      181 aaactggata ccccctttat tcattgaacg caacgagcat ttgtctgcga aaaatccggg
      241 gacggaccat atcatctggc aggcgtgggt ggcaaaaaaa gcggggcaga tagttgggcg
      301 tattaccgcg caaatagata ccttgcaccg tgagcgttac ggtgaagata ccggtcattt
      361 cggcatgatt gacgccattg atgatccgca ggattttgcc gcgttgtttg gcgcagcgga
      421 agcgtggttg aagtcacaag gtgcaagtaa gatcagcggg cctttcagcc tgaatatcaa
      481 tcaggaaagc ggattactga ttgaaggttt tgacacacca ccctgtgcga tgatgccaca
      541 cggcaaaccg tggtatgccg cgcatattga acaactgggt tatcacaaag gcattgattt
      601 actggcgtgg tggatgcagc gaaccgatct cactttctct ccggcgctaa aaaaactgat
      661 ggatcaggtg cgcaaaaagg tgaccattcg ctgcatcaat cgtcagcggt ttgccgaaga
      721 gatgcagatc ctgcgtgaga ttttcaactc tggctggcag cacaactggg gatttgtgcc
      781 gtttaccgaa catgaattcg cgaccatggg cgaccaactt aaatatctgg tgccggacga
      841 tatgatctac atcgctgaga ttgattctgc accctgcgcg tttattgtgg cttgcctaac
      901 atcaacgagg cgattgccga tctgaacgga tcgctctttc ccttcggctg ggcaaaattg
      961 ctgtggcgtt tgaaagtcag cggtgtgcga actgcgcgag taccgctgat gggcgtgcgc
     1021 gacgagtatc agttcagccg catcggcccg gtgattgcgc tgttattgat tgaagcctta
     1081 cgcgatccgt ttgcccgccg gaagatcgat gcgctggaga tgtcatggat cctcgaaacc
     1141 aatacaggca tgaataacat gctggaacgc attggggcgg agccgtacaa gcgttaccga
     1201 ttgtatgaaa agcaaattta ataccagatc ctgtttagcg caggggcggt cattcgggag
     1261 agggtataga tgaacagatt taacaccaga tcctgttaag tgcaggggcg gtcccctcgc
     1321 cttggggata gggtaaaact ctgctcacat tcccaccttg ttcatctcca gcggcaccgc
     1381 cgagcgaatc aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg
     1441 tacatgatcg cgcgctgcgc gctgagcgac gtgcggcagc cgctgcaaca ccgcgttgta
     1501 gatccgcgca tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt
     1561 tacgctggca aacactgaat taaacatcag atcggtcaat gattgcagcg taaacaccag
     1621 cacctgattg tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg
     1681 ttcgatcagc gaaatctctt tgtggttctc actggcggcg agcatttttt cataacagcg
     1741 ggttatcaca acaaaatcag cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc
     1801 ctccagtaat gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tactgaacag
     1861 atggatcagc gggctggtgt cctgctcccg attaagccgt gcgacatgag aatcgcgacc
     1921 ctgcgccgtt tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga
     1981 gcgtgagaag ccgagctttt cacacagtcg acgttccgag ggaagcggct gaccgacctt
     2041 cagtacgccg tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg
     2101 acgttcatct ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac
     2161 tcgcaaaaca cgcacatcac gcaagtgatt atgttacatc aatttaacat taagttaacc
     2221 aagacaaggt cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga
     2281 aaacgaaaag cccgcttaat aactgttcac agaagcagcg cgcaaaaatc agctgccaca
     2341 caacacaaca aagcgaagcc tactcatgag catcttgtac gaagagcgtc ttgatggcgc
     2401 tttacccgat gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctggact
     2461 tgagatcctg cataccgatg aggagatcat tccttacgag tgtgacgggt tgagcgcgta
     2521 tcgcacgcgt ccattactgg ttgttctgcc taagcaaatg gaacaggtga cagcgattct
     2581 ggctgtctgc catcgcctgc gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc
     2641 tggtggcgcg ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagag
     2701 cctcgacatt aaccccgttg gtcgccgcgc gcgcgtgcag ccgggcgtat gtaacctggc
     2761 gatctcctag gccgttgcgc cgcataatct ctattacgcg ccggatcctt cctcacaaat
     2821 cgcctgttca attggcggca atgtggcgga aaatgccggt ggcgtccact gcctgaaata
     2881 tggtctgacc gtacataacc tgctgaaaat tgaagtgcaa acgctggacg gcgaggcgct
     2941 gacgctggga tcggacgcgc tggattcacc tggttttgac ctgctggcgc tgttcaccgg
     3001 atcggaaggt atgctcggcg tgaccaccga agtgacggta aaactgctgc cgaagccgcc
     3061 cgtggcgcgg gttctgttag ccagctttga ctcggtagaa aaagccggac ttgcggttgg
     3121 tgacatcatc gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat
     3181 ccgcgcggcg gaagatttta ttcatgccgg ttatcccgtc gacgccgaag cgattttgtt
     3241 atgcgaactg gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaactacat
     3301 cttgttgaaa gcgggcgcga ctgacgtccg tttggcacag gacgaagcag agcgcgtacg
     3361 tttctgggcc ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc cggattacta
     3421 ctgcatggat ggcaccatcc cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg
     3481 tttatcgcag caatatgatt tacgtgttgc caacgtcttt catgccggag atggcaacat
     3541 gcacccgtta atccttttcg atgccaacga acctggtgaa tttgcccgcg cggaagagct
     3601 gggcgggaag atcctcgaac tctgcgttga agttggcggc agcatcagtg gggaacatgg
     3661 catcgggcga gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg aaatcacgac
     3721 cttccatgcg gtcaaggcgg cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat
     3781 tcccacgcta caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc
     3841 ttttcctgaa ctggagcgct tttgatgcta cgcgagtgtg attacagtca ggcgctgctg
     3901 gagcaggtga atcaggcaat tagcgataaa acgccgctgg tgattcaggg cagcaatagc
     3961 aaagcctttt taggtcgccc agtcaccggg caaacgctgg atgtgcgttg tcatcgcggc
     4021 attgttaatt acgacccgac cgagctggtg ataaccgctc gtgccggaac gccgctggtg
     4081 gcgattgaag cggcgctgga gagcgccggg caaatgcttc cctgcgagcc gccgcattat
     4141 ggtgaagaag ccacctgggg cgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg
     4201 tggagcggtt cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cactggaaaa
     4261 catctgcgtt ttggtggcga agtgatgaaa aacgttgccg gatacgatct ctcacggtta
     4321 atggccggaa gctacggttg tcttggcgtg ctcactgaaa tctcaatgaa agtgttaccg
     4381 cgaccgcgtg cctctctgag cctgcgtcgg gaaatcagcc tgcaagaagc catgagtgaa
     4441 atcgccgagt ggcaactcca gccattaccc attagtggct tatgttactt cgacaatgcg
     4501 ttgtggatcc gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga actgctgggt
     4561 ggcgaagagg ttgtgggtca gttctggcag caattgcgtg aacaacaact gccgttcttc
     4621 tcgttaccag gtaccttatg gcgcatttca ttacccagcg atgcgccgat gatggattta
     4681 cccggcgagc aactgatcga ctggggcggg gcgttacgct ggctgaaatc gacagccgat
     4741 gacaatcaaa tccatcgcat cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc
     4801 ggagatggtg gctttgcccc gctatcggct cctttattcc gctatcacca gcagcttaaa
     4861 cagcagctcg acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga actttgagga
     4921 gcaggctatg caaacccgat taaccgaaga gatgcggcag aacgcgcgct ggaagccgac
     4981 agcatcctgt gcgcctgtgt tcactgcgga ttttgtaccg caacctgccc aacctatcag
     5041 cttctgggcg atgaactgga cgggccgcgc gggcgcatct atctgattaa acaggtgctg
     5101 gaaggcaacg aagtcacgct taaaacacag gagcatctcg atcgctgcct cacttgccgc
     5161 aattgtgaaa ccacctgtcc ttctggtgtg cgctatcaca atttgctgga tatcgggcgt
     5221 gatattgtcg agcagaaagt gaaacgccca ctgccggagc gaatgctgcg cgaaggattg
     5281 cgccaggtag tgccgcgtcc ggcggtcttc cgtgcgctga cgcaggtagg gctggtgctg
     5341 cgaccgtttt taccggaaca ggtcagagca aaactgcctg ctgaaacggt gaaagctaaa
     5401 ccgcgtccgc cgctgcgcca taagcgtcgg gttttaatgc tggaaggctg cgcccagcct
     5461 acgctttcgc ccaacaccaa cgcggcaact gcgcgagtgc tggatcgtct ggggatcagc
     5521 gtcatgtcag ctaacgaagc aggctgttgt ggcgcggtgg actatcatct taatgcgcag
     5581 gagaaagggt tggcacgggc gcgcaataat attgatgcct ggtggcccgc gattgaagca
     5641 ggtgccgagg caattttgca aaccgccagc ggttgcggcg cgttcgtcaa agagtatggg
     5701 cagatgctga aaaacgatgc gttatatgcc gataaagcgc gtcaggtcag tgaactggcg
     5761 gtcgatttag tcgaacttct gcgcgaggaa ccgctggaaa aactggcaat tcgcggcgat
     5821 aaaaaactgg ccttccactg cccgtgtacc ctacaacatg cgcaaaagct gaacggcgaa
     5881 gtggaaaaag tgttacttcg tcttggattt accttaacgg acgttcccga cagccatctg
     5941 tgctgcggtt cagcgggaac atatgcgtta acgcatcccg atctggcacg ccagctgcgg
     6001 gataacaaaa tgaatgcgct ggaaagcggc aaaccggaaa tgatcgtcac cgccaacatt
     6061 ggttgccaga cgcatctggc gagcgccggt cgtacctctg tgcgtcactg gattgaaatt
     6121 gtagaacaag cccttgaaaa ggaataaaaa aatgaaaact aaagtcattc ttagccagca
     6181 aatggcgagt gcaattattg ccgcaggtca ggaagaggcg cagaaaaata actggtctgt
     6241 ttccattgct gttgccgatg acggcggtca tctgctggcg ttaagtcgca tggacgattg
     6301 cgcgccgatt gcggcttaca tctcccagga gaaagcgcgt accgccgcgc tggggcgtcg
     6361 tgaaactaag ggctatgaag agatggtgaa caacggacgt accgcgttcg tgactgcgcc
     6421 gttactgacg tcgctggaag gcggcgtacc ggttgttgtt gatgggcaaa ttattggtgc
     6481 cgtgggcgtt tctggtttaa ccggagcaca ggatgcgcag gtcgcgaaag tggcagcagc
     6541 ggtgttggcg aaataagcga aaacgaggag agaaacaatg agtcaaacca taacccaggg
     6601 ccgtttacgc attgacgcca attttaaacg ttttgtggat ggagaagttt tgccgggcgt
     6661 ggaactggac gctgctgcat tctggcacaa tgttgatgag atcgttcacg atctggcacc
     6721 agaaaatcgt cagttgctgg cagaacgcga tcgcattcag gcggcgcttg atgagtggca
     6781 tcgcagcaat ccggggccgg taaaagataa agcggcctat aaatctttct ttcgtgaact
     6841 gggctacctg gtgccgcaac cggagcgcgt gacggtggaa accacgggca ttgacagcga
     6901 aatcaccagt caggcggggc cacagctggt ggttccggca atgaacgccc gctacgcgct
     6961 gaacgcggcg aacgctcgct ggggctcact gtacgatgcg ttatacggca gcgacatcat
     7021 cccgcaggaa ggggcgatgg tcagtggcta cgatccgcag cgcggtgagc aggttatcgc
     7081 ctgggttcga cgtttcctcg atgaatcact accgctggaa aacggcagct atcaggatgt
     7141 ggtggcgttt aaggtggtcg ataaacaatt acgcatccag ttgaaaaatg gtaaagaaac
     7201 cacgttacgt actccagcac agtttgtcgg ttaccgtggc gatgccgctg cgccgacctg
     7261 cattttgctg aaaaataacg gcctgcatat tgaactgcaa attgatgcca acgggcggat
     7321 tggcaaagac gatccggcgc acatcaacga tgttatcgtc gaagctgcta tcagtaccat
     7381 tctcgactgc gaagattcgg tcgcggcggt tgatgcggaa gataaaatcc tgctgtaccg
     7441 caatctgctg ggcctgatgc aggggactct gcaagagaaa atggagaaaa acggtcggca
     7501 aatcgtacgt aaactgaatg acgatcgtca gtacaccgcc gccgatggct ctgaaatttc
     7561 tctgcacgga cgctcgttgc tgtttatccg caacgtgggt catttgatga ccattcctgt
     7621 gatttgggac agcgaaggca atgaaatccc ggaaggtatt cttgatggcg tcatgactgg
     7681 cgcgattgcc ctctatgatt taaaagtgca gaaaaactcg cgcactggca gcgtctatat
     7741 tgtgaaaccg aaaatgcacg gtccgcagga agtggcgttc gccaacaaac tgtttacccg
     7801 cattgagaca atgctcggta tggcaccgaa taccctgaaa atgggcatta tggatgaaga
     7861 acgccggacc tcgctgaact tgcgtagctg tatcgctcag gcgcgcaacc gcgtggcgtt
     7921 catcaatacc ggtttcctcg accgtaccgg cgatgaaatg cattcggtga tggaagctgg
     7981 cccgatgctg cgtaaaaacc agatgaaatc gacgccgtgg atcaaagcct acgaacgtaa
     8041 taacgtgctt tccggtctgt tctgtgggct gcgcggtaaa gcgcaaatcg gtaaaggcat
     8101 gtgggcaatg ccggacctga tggcagacat gtacagccag aagggcgacc aactgcgtgc
     8161 cggggcaaac acagcctggg ttccgtcacc aaccgctgct acgctccatg cgctgcacta
     8221 ccaccatacc aacgtacaga gcgtgcaagc caacattgcc cagaccgagt tcagtgctga
     8281 atttgaaccg ctgctggacg atctgctgac tattccggtg gctgaaaacg ctaactggtc
     8341 ggcgcaagag atccaacaag agctggataa caacgtgcag gggattctgg ggtacgtggt
     8401 gcgttgggtg gagcagggga ttggttgttc aaaagtgccg gatattcaca acgtggcgct
     8461 gatggaagac cgcgcaacgc tgcgtatctc cagccagcat atcgccaact ggttacgtca
     8521 cggtattctg accaaagaac aggtgcaggc gtcgctggag aatatggcga aagtggttga
     8581 tcagcaaaat gctggcgatc cggcttatcg tccgatggcg gggaatttcg ctaactcgtg
     8641 tgcttttaaa gctgccagcg atttaatctt cctcggcgtg aaacagccaa acggttatac
     8701 cgaaccgtta ttacacgcct ggcgtttacg cgaaaaagaa agtcattaat agcgtcaaaa
     8761 aagccccggc aagttttatt gtcggggcta tgatagtaac tgcggtagta catttatccc
     8821 ggaaataacc ggagcgatta tcagggttta acaatattaa atacccgtag cgcattacta
     8881 gcgggcctaa aacaacaata tggacatctt aacgatactc tgcgcctgcg tgtgcggaac
     8941 agaaaccctt acccgatata cttgttatgc cagaggtagc aattgcgtta gcggcgtact
     9001 gttgctgtaa agtataacgc ctatatttta cagttcagtc ctcttttgct tattacaacc
     9061 ttaagtatat caagcatata aagataataa gagactgaac aatatggtta cctggaccca
     9121 aatgtatatg ccgatgggag gactggggct atccgctctg gtcgccctga tcccgataat
     9181 attcttcttc gttgcactcg cggtattacg tctgaaagga catgttgctg gagcaataac
     9241 ccttatatta tctatcctga ttgcgatatt cgcctttaaa atgccgattg atatggcatt
     9301 tgctgctgcg ggctatggct ttatttatgg attatggcca attgcgtgga ttattgtcgc
     9361 ggcggtgttc ctgtataaat taaccgttgc cagcgggcag ttcgatatta tccgcagctc
     9421 ggttatctcc atcaccgacg atcagcgttt gcaggtgtta ctgattggtt tctcctttgg
     9481 tgcgttgctg gaaggagctg ctggctttgg tgcgacggtg gcgtttggtg cgttgggcgt
     9541 gccgattctg gtcgccggtc aggtaacggg aatcgatccg ttccacattg gcgcaatggc
     9601 gggacgtcag ttaccgttcc tgtcggttct tgtgccgttc tggctggtag caatgatgga
     9661 cggctggaaa ggggtgaaag agacgtggcc agcggcgctg gttgctgggg gaagcttcgc
     9721 tgtcactcag ttctttacct ctaactatat tggtccggaa ctgccggata ttacttcggc
     9781 gctggtgagt atcgtctcac tcgctttatt ccttaaagtc tggcggccga aaaataccga
     9841 aacggcaatc agcatggggc aatccgcagg tgcgatggtg gtaaataagc catcttctgg
     9901 cggtcccgtg ccttcagaat atagtctggg gcaaatcatt cgtgcgtggt caccgttttt
     9961 aatcttaacg gtgctggtca ccatctggac catgaagccg tttaaagcgt tatttgctcc
    10021 gggcggcgcg ttttattcac tggtgattaa tttccagatc cctcatttgc atcaacaagt
    10081 gttgaaagcg gcacccattg tcgcccaacc aacgccaatg gatgcggtgt ttaaattcga
    10141 ccctctctcg gctggcggca ccgctatttt tattgcggcg attatctcta tcttcatcct
    10201 cggcgtgggc atcaagaaag ggattggcgt ctttgccgaa atgctaatta gtctgaagtg
    10261 gccgatattg tcgattggca tggtgctggc attcgccttc gtcaccaact attccggcat
    10321 gtccaccacg ctggcgctgg tactggcagg tacaggcgtg atgttcccgt ttttctcacc
    10381 gtttctcggc tggctgggcg tattccttac cgggtcggac acttcttcaa acgccctgtt
    10441 tggttcactg caatcgacca cggcgcagca aatcaacgtc tctgacactc tgctggtggc
    10501 agcaaatacc agcggcggcg taactggcaa gatgatctcc ccgcaatcta tcgccgtggc
    10561 ctgcgccgcg acgggcatgg tgggccgaga atctgaactg ttccgctaca ccgtgaagca
    10621 cagtctgatt tttgccagcg ttatcaccct gctgcaggcg tatgtgttta ccgggatgtt
    10681 agtctcgtaa atcaataaag ccggatgaag gtaaatatca cccggctttc catttgtctg
    10741 tcgtttatct cgccagggaa ccgtttttac gattggcttc tttatacgta gtctccgttt
    10801 tacgatgcga attgtttaat atttatacaa cttggcttct gtgctggtta taaagtaccc
    10861 actttcatga gatcaatttt agtatgttat tacattgctt catctgttgc tttcatgacg
    10921 aatggcgttt tttctcttat ttatattttt cactagaaca atgagtagat caaaattccc
    10981 aaaactccca aaaacataag attattcgca aagctgattt tgcagaataa tgagaacttt
    11041 taagatttgt cgaggatcaa ttttaagcaa tttatctcca cttgttacat aaaagatgcc
    11101 gttaggttaa taagttaatc ttgctataac agtgttcgtt actgcctatt aataaagata
    11161 tttgggatag ggtaacgtgg atgaggattc gaaatcatcc gtcttgcgga aagtgaaatg
    11221 ttggcagatg ttaatattat gtatgtattt taactccaac aacacaccaa tcattttgtt
    11281 agaaataaga aaatttatgg ttaaataatt tggtgattat gaagcattaa atcgtaatgc
    11341 caaatattgt tcgctttgga gagtattatt gcctgtttgt gtctgttgag cag
//
LOCUS       DAZOZX010000126        11294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZOZX010000126 DAZOZX010000000
VERSION     DAZOZX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.126"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            71..205
                     /locus_tag="ACHUPT_003262"
     CDS             71..205
                     /locus_tag="ACHUPT_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0820705.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            184..288
                     /gene="speFL"
                     /locus_tag="ACHUPT_003263"
     CDS             184..288
                     /gene="speFL"
                     /locus_tag="ACHUPT_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0820706.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            599..2796
                     /gene="speF"
                     /locus_tag="ACHUPT_003264"
                     /pseudo
     CDS             599..2796
                     /gene="speF"
                     /locus_tag="ACHUPT_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2793..4112
                     /gene="potE"
                     /locus_tag="ACHUPT_003265"
     CDS             2793..4112
                     /gene="potE"
                     /locus_tag="ACHUPT_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0820707.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4168..5808)
                     /gene="pgm"
                     /locus_tag="ACHUPT_003266"
     CDS             complement(4168..5808)
                     /gene="pgm"
                     /locus_tag="ACHUPT_003266"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0820708.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5834..6379)
                     /gene="seqA"
                     /locus_tag="ACHUPT_003267"
     CDS             complement(5834..6379)
                     /gene="seqA"
                     /locus_tag="ACHUPT_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0820709.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6564..7328
                     /gene="ybfF"
                     /locus_tag="ACHUPT_003268"
     CDS             6564..7328
                     /gene="ybfF"
                     /locus_tag="ACHUPT_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0820710.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7468..7761
                     /gene="ybfE"
                     /locus_tag="ACHUPT_003269"
     CDS             7468..7761
                     /gene="ybfE"
                     /locus_tag="ACHUPT_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0820711.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7901..8431
                     /gene="fldA"
                     /locus_tag="ACHUPT_003270"
     CDS             7901..8431
                     /gene="fldA"
                     /locus_tag="ACHUPT_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0820712.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8641..8727
                     /gene="uof"
                     /locus_tag="ACHUPT_003271"
     CDS             8641..8727
                     /gene="uof"
                     /locus_tag="ACHUPT_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0820713.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8720..9166
                     /gene="fur"
                     /locus_tag="ACHUPT_003272"
     CDS             8720..9166
                     /gene="fur"
                     /locus_tag="ACHUPT_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0820714.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9250..9576)
                     /gene="chiQ"
                     /locus_tag="ACHUPT_003273"
     CDS             complement(9250..9576)
                     /gene="chiQ"
                     /locus_tag="ACHUPT_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0820715.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9613..11019)
                     /gene="chiP"
                     /locus_tag="ACHUPT_003274"
     CDS             complement(9613..11019)
                     /gene="chiP"
                     /locus_tag="ACHUPT_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR0820716.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2767 a         2778 c         2807 g         2942 t
ORIGIN      
        1 caaatataaa acacggaatt acaacattta agtaacaaaa cagcatctaa tatggcgata
       61 ttcataatta atgatttatt atctggggta tgatcgtttt ttgttgatct tcttcacaga
      121 ttatagccat ttcatggata gaataactct accttcaact gacacagcaa gcggtaaagg
      181 taaatggaaa ataataaccg cttaatgcct catataaggc ggacaaccca tatcatgaag
      241 tttacccatc gtaatagttt tgactttcat ttctttaatg cccgctagtc ttctgactaa
      301 agggcacccc aacgtacagg tctccctgac tttaagcatt acaggttaat acctgtattc
      361 ctcggtgctc atatactgct aaccctttta aactctaaat aattcgagtc gcagcacttg
      421 caacttgagg tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca
      481 aaaagcaaac tactgattta tttatcagcg gtggagcttt gctttttttc cgacgtgatc
      541 gatttctcct ttgagaaatt gaggacctgc tattacctga aataaagaga tgaataaaat
      601 gtcagaatta aaaattgccg ttagccgttc ttgcccggat tgtttttcca ctcatcgtgc
      661 atgcgtgaat attgacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcaa
      721 tgatgttgaa cgtggaaaac tcgataaaat agacgctact ggctatggca ttcctgtttt
      781 tattgcaacg gaaaatgaag aacgtgtacc agcagaatat ctgccacgta tttctggtgt
      841 cttcgagcat tgcgaatcac ggaaagagtt ttatggacgt cagttagaaa ccgctgctag
      901 ccattatgaa acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgtcaatca
      961 gggcaatagc gcttttgact gcccgggtca tcagggaggt gagtttttcc gtcgtcatcc
     1021 ggcagggaat cagttcgtgg agtacttcgg agaaacgctg ttccgttccg acttatgcaa
     1081 cgcagacgta gcaatgggcg atctgctgat tcacgaaggc gcgccatgca ttgcacagca
     1141 acatgcggca aaagtgttta atgccgataa aacatacttc gttctaaatg gcacttcatc
     1201 ttctaacaaa gtggttttaa acgccctgct aacaccgggt gatctggtgc tgtttgatcg
     1261 caataaccac aaatctaacc accacggagc gttgctacag gctggtgcaa caccggttta
     1321 tctggaaacg gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga
     1381 agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg cagcgggcaa aagaggctcg
     1441 tcctttccgc ctcgctgtga ttcagttagg cacctacgac ggtacgattt ataacgcccg
     1501 ccaggtggtg gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt
     1561 tggctatgag caatttatcc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa
     1621 tgagaacgat ccgggtattc tagttacgca atccgtacat aaacagcagg ctggtttttc
     1681 tcaaacttca caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc
     1741 gcacaaacgc atgaacaacg cctttatgat gcacgcctcc accagcccgt tctatccgct
     1801 gtttgccgca ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg
     1861 gatggattgt gtggtaaatg gcattaatgc ccgcaaactg atcctcgata actgtcagca
     1921 tattcgtccg ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc
     1981 gcaaattgcg gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcattcttt
     2041 tgaaggctat gcagagaatc aatactttgt cgatccttgc aaactgttgc tgacaacccc
     2101 aggtattgat gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc
     2161 taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaacg ccatcctctt
     2221 cctgctgact ccggcagaag atatggccaa acttcagcaa cttgtgccct gctggtacgc
     2281 ttcgaaaaac tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa
     2341 cagcatgaag agcgctacgc cggttatacc ctgcgtcagt tgtgccagga aatgcatgat
     2401 ttgtatgccc gccacaacgt gaaacaactg caaaaagaga tgttccgcaa agcgcacttc
     2461 ccacacgtca gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag
     2521 ctggttcgtc taccggatgc cgaaggtcgt atcgctgccg aaggggcgct tccttatcct
     2581 ccgggtgtgc tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc
     2641 agcgctctgg aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc
     2701 tatatcgaag aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat
     2761 gcgcaaagca ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg
     2821 gcgtcgttca gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc
     2881 tgccgacaaa gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg
     2941 taggctcaat ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag
     3001 gtggtatggg cggttatgcc gagtatgctt tcggtaaatc cggtaacttt attgcgaact
     3061 atacctacgg cgtctcactg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt
     3121 acggcaccga actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg
     3181 gggtgctgtg gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca
     3241 gtagcattac cgtgtggggg ggcattattc cggtggtagg cctgtgcatt atcggctggt
     3301 tctggtttag cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca
     3361 gtgcagtagg ttcttccatc gctatgacgc tgtgggcttt tcttggtctg gagtctgcgt
     3421 gtgcgaatac tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg
     3481 gtacgttagg tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc
     3541 ctaatatgga gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttta
     3601 caccagaagt gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc
     3661 ttggctggca gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc
     3721 ctaaaatttt ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg
     3781 tgattatcca gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca
     3841 acgtgctggt taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg
     3901 cattagtcat tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact
     3961 ttgttgcttt tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag
     4021 ccatgctgta cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac
     4081 cacgctttga actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg
     4141 cgaccgctct ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc
     4201 aaccgcttct ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat
     4261 cttatatgcg tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac
     4321 tttcagaccg ccaatagaag caccgttgcc cggagcagca gtcaggcgcg cggtgatcgg
     4381 gtcacctgcc agggtgctgg cgctcaccat ttccggagac agcttagaca gcgccgcttt
     4441 ttgtgcggaa gtcgcagctg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc
     4501 cagttcgttg tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag
     4561 cagacacata atgatgccgt ctttgtcggt ggaccacggc gtgccgtcga aacgcaggaa
     4621 ggaagcccct gcgctctctt cgccgccaaa gccgaagctg ccgtcgaaca gaccatcaac
     4681 aaaccatttg aaacctaccg gtacttctac cagcttacgg cccaagtcat tgaccacacg
     4741 gtcgatcatc gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg
     4801 acgatgctgg aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc
     4861 tgccggagtg acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa
     4921 cttatcacgc agtgccagca gacccgccat cgcacactcg gaggagcagt ccatacggat
     4981 cgcaccgtct ttatcgaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat
     5041 ggtcaggttg aggttgtaat actcgccaat gcgcttccag tattcgatac cggaaccgcc
     5101 caacggatca acgcccagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc
     5161 ggccagccct tccacgaacg gctgcaccag atcctgctct ttcacatagc cggatgccat
     5221 cgcttcgtcg agggagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc
     5281 cttgtcttcc accactttgg tgacgttagt atcagccggg ccaccatttg gcggattgta
     5341 cttgatacca ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag
     5401 cgggccacct tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa
     5461 gccattgttt tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga
     5521 aatgaacgca ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat
     5581 gccgtttttc gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt
     5641 aaagctgtgg cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc
     5701 cgcattccct gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat
     5761 caaatcactc tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt
     5821 tgtctgcaac gttttagata gttccgcaaa ccttctcaat caattccgcc gggaattgca
     5881 tcgactgcat gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca
     5941 cccaatacgg cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg
     6001 tttgttcatc tgccgcaaag tacacgcgtg tacgaccgtg caacgattcc gttgcttcgg
     6061 caaacgcctg ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg
     6121 ctcgcttttg ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt
     6181 ctttaatcgt tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt
     6241 tcgtcaccgg agcagcaggc tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt
     6301 cggatgcgct ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat
     6361 caacttcaat cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccaa
     6421 gagtatatga ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg
     6481 aataaacctt atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac
     6541 aacgcgaaaa aaagaacttt gccatgaaat taaatatccg cgcgcaaact gcacaaaacc
     6601 agcacaataa ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg
     6661 gcgtactggc tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc
     6721 acggtctttc accgagagag ccggtaatga attacccggc gatggcgcag gatcttgttg
     6781 ataccctgga tgcactgcag atcgacaaag caacatttat cggtcactcc atgggcggta
     6841 aagcggtaat ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg
     6901 atatcgcacc ggtcgactat cacgtacgcc gtcatgatga gattttcgca gctatcaacg
     6961 cggtcagtga atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc
     7021 ttaatgaaga aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct
     7081 ttaacgtgcc ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg
     7141 catgggatca ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt
     7201 accgtgatga tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg
     7261 gtcactgggt ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca
     7321 atgattaacg caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg
     7381 gcgtgactca tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg
     7441 attgtttccc ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta
     7501 gatctgttcg cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat
     7561 gaacagctgc gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag
     7621 cgtgtcgaac tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggaatcg
     7681 cgtaatatga gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg
     7741 cgtagtcagg gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg
     7801 tttgctgatt ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt
     7861 ccacccccat ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt
     7921 tttcggcagc gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg
     7981 taaagacgtt gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta
     8041 cgacattctg ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga
     8101 tgacttcttc ccgactctcg aagagattga tttcaatggt aaactggttg cgctgtttgg
     8161 ttgtggtgac caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga
     8221 catcattgaa ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt
     8281 cgaagcatca aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga
     8341 ccgtcagccg gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga
     8401 gttgcatctc gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc
     8461 cgtattaata gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt
     8521 acctatacaa tgtcccagtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg
     8581 tttttattaa caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata
     8641 atgatacgca ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta
     8701 gtaacaggac agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa
     8761 agtaacgctt cctcgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt
     8821 cagtgcggaa gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac
     8881 ggtatatcgc gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga
     8941 aggcggtaaa tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct
     9001 cgactgcggc aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat
     9061 tgccgcaaaa catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc
     9121 cgaaggcgat tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga
     9181 gaaaagccaa cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac
     9241 agctctcact tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt
     9301 caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg
     9361 tacactttcc tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac
     9421 gctctggcag gcttcaattt tttctggcga accttgcgcg gtgttgatac aggcgctgta
     9481 tgcctctttc agacggctgt cttcctgtgg tgcggttgat tgcgcacaag ctaccagccc
     9541 cgatgccatc atggcgatga gaatcagttt tttcattgaa tgcggcaggc acaaaacctg
     9601 ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcacgctc
     9661 atcctggaag atgttaccgt aaccgccgcc ccagcttggg atgtcggagt ggttgtcgta
     9721 ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtgtagac
     9781 cgcatccagg ctgtaggcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg
     9841 attgctctgc caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc
     9901 gaagcctgga agattccagt ttttcaggtc atacatcgca ctgaagaaga ccgctttttc
     9961 gccgttagcg ttgaagtcgg aacggttatc ccaccagata tccaggcggc cgttggagga
    10021 agcgtaggtt ggggtcatac gttgcaggaa gtatccctgc tgactgtcag ccttaatcca
    10081 ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg taaccaaagg tcaacgcttg
    10141 cagccaggcg gtgccgtcat aaagatcgtt gacgctgcga tcgtcaactt tatcgcgggt
    10201 accatagaac tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc
    10261 tttggcaaaa tattgatcga tatacccttc cgcctgacca aatgccgctt ccagtacgaa
    10321 gttatttttg aagtcgtatt tcgcaccgat ggagtgcaga taatcaactt tggtggtttt
    10381 atcgttctga taaaactcat ccatttccag atgccacggc gctttgtatt cgttggtcca
    10441 catgtaggag aaactcaagg caccagcatc gccgtaatca aaattcgccc cggcttccgc
    10501 cccctgataa gtacccggca taaagctcca gtgcggcgct aacagcgttt gaccggttgg
    10561 ctgaatgtaa cctgccctcg cccaaaccgg accgtattta aatttggccg cagctttata
    10621 caggcttata ccgcttttgt cgccggacca gtcttcgtca taggctttat tactttttga
    10681 aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc gccatttcaa tcgccgtaaa
    10741 cgcagcaata tcaaggccga acatatcagc agcataaccg gactgaaaat cgaggttggc
    10801 attccaggtg gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg
    10861 gtcgcgttca cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc
    10921 ttcggcgcga gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcaatagc
    10981 cagcgccagc gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta
    11041 taaattgctg caccaaaggt gaacagcgaa aaaaaataaa aattaaaaaa gtagcgttga
    11101 gaaaagtctc gctgactaca ggaacagact atttttcgcg acgcgaatta tcaatctctt
    11161 tctgtaacca gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa
    11221 ggcttaagtt tgcggttgaa aggcttgcag aagaataaaa acgtgaaatt ccagcaagat
    11281 gaaagcggtt acat
//
LOCUS       DAZOZX010000127        11012 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZOZX010000127 DAZOZX010000000
VERSION     DAZOZX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.127"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            127..2355
                     /gene="crfC"
                     /locus_tag="ACHUPT_003275"
     CDS             127..2355
                     /gene="crfC"
                     /locus_tag="ACHUPT_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0820717.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2352..3230
                     /locus_tag="ACHUPT_003276"
     CDS             2352..3230
                     /locus_tag="ACHUPT_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFR0820718.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            3494..4996
                     /gene="proP"
                     /locus_tag="ACHUPT_003277"
     CDS             3494..4996
                     /gene="proP"
                     /locus_tag="ACHUPT_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0820719.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5108..5197
                     /gene="pmrR"
                     /locus_tag="ACHUPT_003278"
     CDS             5108..5197
                     /gene="pmrR"
                     /locus_tag="ACHUPT_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0820720.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5173..6273)
                     /gene="pmrB"
                     /locus_tag="ACHUPT_003279"
     CDS             complement(5173..6273)
                     /gene="pmrB"
                     /locus_tag="ACHUPT_003279"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0820721.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6274..6942)
                     /gene="basR"
                     /locus_tag="ACHUPT_003280"
     CDS             complement(6274..6942)
                     /gene="basR"
                     /locus_tag="ACHUPT_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0820722.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6939..8582)
                     /gene="eptA"
                     /locus_tag="ACHUPT_003281"
     CDS             complement(6939..8582)
                     /gene="eptA"
                     /locus_tag="ACHUPT_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0820723.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8686..10023)
                     /gene="adiC"
                     /locus_tag="ACHUPT_003282"
     CDS             complement(8686..10023)
                     /gene="adiC"
                     /locus_tag="ACHUPT_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0820724.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10160..10921)
                     /gene="adiY"
                     /locus_tag="ACHUPT_003283"
     CDS             complement(10160..10921)
                     /gene="adiY"
                     /locus_tag="ACHUPT_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0820725.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
BASE COUNT         2694 a         2696 c         2869 g         2753 t
ORIGIN      
        1 ctgataaaaa aacaacacac taaatataaa attatttctt gcagtaatta taaaagccga
       61 aaacagaact caaaaacaat ctggctaaat aaaagtaaca aaatttgctt taaggaagaa
      121 ttttctatgt acacacagac cctgtatgag ttaagtcagg aggctgaacg cctgttacag
      181 ctttctcgcc aacagttgca gttactggaa aaaatgcctc tctctgtacc cggagacgac
      241 gcgccacaac tggctttacc ctggagtcag cctaatatcg ccgaacgtca cgcgatgctg
      301 aataatgagt tgtgtaaaat ttcccgactg gaaatggtgc tggcaattgt cggtaccatg
      361 aaagcaggga aatcaaccac cattaatgcc attgttggta cggaggttct gcctaatcgt
      421 aatcgcccaa tgactgcgct gccgacgctt attcgccata cgcccgggca aaaggaaccg
      481 gtactgcatt tttcacatgt cgcgccaatc gattgtttaa ttcaacaatt acaacagcgc
      541 ctgcgtgatt gcgatattaa gcatctgacc gatgtgctgg aaatagataa agatatgcgt
      601 gcgcttatgc agcggatcga aaatggcgtc gctttcgaaa aatattatct gggtgcccag
      661 cctatttttc attgtctgaa aagtttgaat gatttagtgc gactggcgaa ggcgctggac
      721 gtcgattttc ctttttctgc ttacgccgcc attgagcata ttcccgtgat tgaagtggag
      781 tttgtccatc tggcggggct ggaaagttat cccggtcagt tgacgttact ggataccccc
      841 gggccaaatg aagccgggca accgcatctg caaaaaatgc ttaaccagca gctggcacgc
      901 gcctcggcgg tactggcggt gctggattat acgcaactga aatcgatctc cgatgaagag
      961 gtccgtgagg cgattttggc ggtggggcaa tcggtgccgc tgtatgtgct ggtcaataag
     1021 ttcgatcaac aggatcgtaa cagtgacgac gccgaccagg tgcgggcact gatttccggg
     1081 acgctgatga aaggctgtat tacgccacag cagatattcc cggtgtcgtc gatgtggggc
     1141 tacctggcga atcgggcgcg ctatgagtta gccaacaacg gtaagttacc agcgccagag
     1201 caacaacgct gggtggaaga ttttgcccat gccgcgctcg gcaggcgctg gcgtcatgcc
     1261 gatctggcgg acctcgaaca tattcgtcat gctgccgatc agttgtggga agattcgctg
     1321 ttcgcccagc caattcaggc gttgcttcat gccgcttacg ctaacgcctc attgtatgct
     1381 ctgcgatctg ctgcgcataa actgttgaat tacgcgcagc aggcgcggga atacctggat
     1441 tttcgtgcgc acgggttaaa cgtcgcttgt gaacaattgc ggcaaaatat ccatcaggtc
     1501 gaagaaagtt tgcagctatt gcaactcaat caggcgcagg tgagcggcga gattaaacat
     1561 gaaatcgagc tggccctgac ctccgccaac cactttctgc gtcaacagca agatgcggtg
     1621 aatgcgcagt tagccgcctt gtttcaggat gattcggagc cgttaagcga gattcgtacc
     1681 cgctgtgaga cactgttaca gacggcgcag aacaccatca gtcgcgactt tacgctgcgt
     1741 tttgccgaga tggaatccac cctttgccgg gtgttaaccg atgttattcg ccccattgag
     1801 caacaagtca aaatggaatt gagcgagtca gggtttcgtc ctgggtttca ttttcctgtt
     1861 tttcacggcg tagttcccca cttcaacact cgccagctgt tcagtgaagt catttcgcgc
     1921 caggaagcaa cggacgagca gagcacgcgt ttaggcgttg tgcgtgagac tttttcgcgc
     1981 tggttgaatc agcccgactg gggacgggga aatgagaaat ccccgacaga aacggttgat
     2041 tacagtgtgt tgcaacgagc attaagcgca gaagtcgatc tctattgcca acaaatggct
     2101 aaagttctgg cagagcaggt cgatgaatct gttacggcag gcatgaatac ttttttcgct
     2161 gagttcgctt catgtttgac agaattacag acgcgtttac gcgaaagtct ggctctgcgt
     2221 caacaaaatg aatcggtggt caggctgatg cagcagcaat tgcagcagac tgtgatgact
     2281 cacagctgga tttacaccga cgctcagctg ttacgcgatg atattcaaac acttttcacg
     2341 gcagaacgat attgaccaag acgttactgg acggccccgg tcgcgtgctg gagtcggttc
     2401 atccgcgctt tttagtggat ctggcgcagg gcgatgatgc ccgccatcct caagcccatc
     2461 agcagcagtt tcgtgaacga ctgatgcagg aacttctttc gcgtgtgcag cttcagacat
     2521 ggacgaacgg cggcatgtta aatgcgccgc ttagcctgcg actgacaatg gtggaaaaac
     2581 tggcgtcgat gctggatccc ggtcatctgg cgctgacgca gatcgcgcag catctggcgc
     2641 tgctgcaaaa aatggatcac cgccagcact ctgctttccc ggagctcccc cagcaaattg
     2701 ccgccttgta tgagtggttt tcagcccgtt gtcgctggaa ggaaaaggcg ttaacgcaac
     2761 gaggcctact ggtgcaggca ggtgatcaga gcgagcaaat ttttacccgc tggcgtgctg
     2821 gggcgtataa cgcctggtcg ttgcctgggc gctgttttat cgttctggag gagttgcgct
     2881 ggggtgcatt tggcgatgcc tgccgtctgg gaagcccgca agcggtggtg ttgttgctgg
     2941 gtgatttgcg cgagaaagcg acacaacatc tggcagagag tatcaatgcg gcaccgacta
     3001 cgcgtcacta ttaccatcag tggtttgcct cttcgaccgt tccgacgggc ggggatcatg
     3061 ctgatttttt aagttggctg ggaaagtgga ccacggcaga taaacaaccc gtttgctggt
     3121 cagtgaccca acgctggcaa actgtcgcgc tggggatgcc acgactctgt tcagcgcagc
     3181 gtttggtggg ggcaatggtc gaggaaatct tctctgtaaa tttggcgtaa ataatcagtt
     3241 acatcaatga gtcctaaacg aaatccatgt gtgaagttga tcacaaattt aaacactggt
     3301 agggtaaaaa ggtcattaac tgcccaattc aggcgtcaac tggtttgatt gtacattcct
     3361 taaccggagg gtgtaagcaa acccgctacg cttgttacag agattgcatc ctgcaattcc
     3421 cgctcccctt ttgcggccgt cgcgctgatt tttctggcgt ttgcggaaat gggccaactc
     3481 tgcgaggaaa gctatgctga aaaggaaaaa agtaaaaccg attacccttc gtgatgtcac
     3541 cattattgat gacggtaaac tgcgtaaagc cattaccgca gcatcactgg gtaatgcaat
     3601 ggaatggttt gattttggtg tttatggttt tgttgcttac gcattaggta aagttttttt
     3661 cccgggggct gaccccagcg tgcagatggt tgctgcactt gccactttct ccgttccctt
     3721 tctgattcga ccgcttggcg gactcttctt tggtatgttg ggcgataaat atggtcgcca
     3781 gaagatcctc gctatcacta ttgtgattat gtcgatcagt acgttctgta ttggcttaat
     3841 accgtcctac gacacgattg gtatttgggc accgattctg ctgttgatct gtaagatggc
     3901 acaaggtttc tcggtcggtg gtgaatatac cggggcgtcg atatttgttg cggaatactc
     3961 ccctgaccgt aaacgtggct ttatgggcag ctggctggac ttcggttcta ttgccgggtt
     4021 tgtgctgggt gcgggcgtgg tggtgttaat ttcgaccatt gtcggcgaag cgaacttcct
     4081 cgactggggc tggcgtattc cgttctttat tgctctgccg ttagggatta tcgggcttta
     4141 cctgcgccat gcgctggaag aaactccggc gttccagcag catgttgata aactggaaca
     4201 gggcgaccgc gaaggtttgc aggatggccc gaaagtctcg tttaaagaga ttgccactaa
     4261 atactggcgc agcctgttga catgtattgg tctggtaatt gccaccaacg tgacttacta
     4321 catgttgctg acctatatgc cgagttattt gtcgcataac ctgcattact ccgaagacca
     4381 cggggtgctg attattatcg ccattatgat cggtatgctg tttgtccagc cggtgatggg
     4441 cttgctgagt gaccgttttg gccgtcgtcc gtttgtgcta cttggtagtg ttgcactgtt
     4501 tgtgttggcg atcccggcgt ttattctgat taacagtaac gtcatcggcc tgatttttgc
     4561 cgggttactg atgctggcgg tgatccttaa ctgctttatg ggcgttatgg cttctacctt
     4621 gccagcgatg ttcccgacgc atatccgtta cagcgcgctg gcggcggcat ttaatatttc
     4681 ggtgctggtt gccggtctga cgccaacact ggcggcctgg ctggtcgaaa gctcgcagaa
     4741 tctgatgatg cctgcctatt acctgatggt agtggcggtg attggtttaa tcaccggcgt
     4801 aaccatgaaa gagacggcaa atcgtccgtt gaaaggtgcg acaccggcgg cgtcagatat
     4861 acaggaagcg aaggaaattc tcgtcgagca ttacgataat atcgagcaga aaatcgataa
     4921 tattgaccac gagattgccg atttgcaggc gaaacgtacc cgcctggtgc agcaacatcc
     4981 gcgaattgat gaataagctg aaacggatgg cctgatgtga cgctgtctta tcaggccaat
     5041 tgaactctta aggttcactt aatctctgac gcgcatactc tcctccaggt taacggagga
     5101 gagtgcaatg aaaaaccgtg tttatgaaag tttaactacc gtgttcagcg tgctggtggt
     5161 cagcagcttt ctttatatct ggtttgccac gttctgatct ttcttcagcc gtacccaggc
     5221 ccgcgtgccg gaagtctctt gccggttttg caggaaaaac tgcccgtgat gcaactgtgt
     5281 aatgcggctg acaatactta accccagacc aatcccgcca taacggctgt ccatacgtac
     5341 aaacgcttta ctcaactccc cgcatttact ctcatcaata cctggtcctt catcttcaac
     5401 tgccatgacc gctccgccat cttcttgcag cttaatcata atgttgctgc cttgcgggct
     5461 gtaacgatgg gcgttttcta ccaggtttcg caataacatc cgcagcaggg ttgcatcacc
     5521 ctgaacggtg atgtcggcgg cgctctctgg caatagcagg gtttgctgtc gctggtcgag
     5581 catggtactg agttcgtcat acgaggggag aatgacatct tccagcagtt ttacatgttg
     5641 ataattaccg gaagaaaatg actgtccggc acgcgccagt tgcagcagct gggagacgct
     5701 ctccatcatc tgatcaagcc gtgccactaa cggtgctaca tcaatgtgat gcgttttcgc
     5761 cagcagttcc agatgcaaac gcacccccgc cagtggcgtt cgcagttcgt gcgcgacgtc
     5821 agcggtaaac aacctttcgt tatccagcgt gctggtcagg cgactgacca gatcgttcaa
     5881 cgccgaaacc accgcttcga tttcgagggt ggcgctgtga atggcaatgg gcgttaagtt
     5941 gtcggcggtg cgcgcttcca gctctttttg cagctccgcc agcgggcggg tgatgcggcg
     6001 taccgcctga tagcagataa atagcgtcag gctgaccata aagacgccgg ggacaatcag
     6061 gctggcgacc gcctcgcgga tctcacgcat gatgtggcga tcgttgttgc gattgtcgcg
     6121 tagcgcctgc tcaaacagct gaatctgctc ggtactttca tgccatagcc agaagacgct
     6181 gatcagctca aacaccaaca aaatggcccc gatggtcaat atcagccgtt ggcgcagcga
     6241 tataggtcgg cgcagaaaac gcatcagatt caattagttt tcctcattcg cgaccagcat
     6301 atagccaaag ccgcgcacgg tgcggatacg ggctttgccc actttgtcgc gcagattgtg
     6361 gatatgcact tccagggtgt tggtcgaggg ttcattgtcc cagttataga tgtcgttgta
     6421 gagaatttcc cgatgtaccg gactgcctgc tttgagcatt aaccgtgaca gcagagcata
     6481 ttctttgggc gtcagaatca actcttcacc gcccatccat acctgacggc gacccatatt
     6541 cagcgtcaga ttgccaacaa tcagctcact ttcgccctga ttattatggc gtcgtagcag
     6601 ggcgcggata cgggcgtgta actcttccag cgcaaaaggc ttcaccagat agtcatcggc
     6661 accgacatcc agcccggcga ttttgtcggt cagcgtatcg cgagcggtga ggatcagtac
     6721 cggcagggtg tattttttct gccggatacg ggcgagaaaa tgcagtccat cttcgtcggg
     6781 taaccctaaa tccagtacca ccaggctgta atgaccgtcc tcaaggcttt gttccgccat
     6841 ccgcgcggtt gtcacgccat cgcacgcgta gccttcggtt tgcgccgcca gaatcagtcc
     6901 ctgcaataac agcgtatcgt cttcaacaat cagaattttc atttactcac tctcctgcaa
     6961 gtttgcagaa tatcatccgc ggcctggtaa tacttcgtct caacgccagt taatcccaat
     7021 agcgtggaga ataaattgtc ttgtgaatag tgttgcgttt gcgcctgttt ttgcaggcag
     7081 ttctggtcaa cctgataccg cttttgataa tcctccgaca gccacagcag cattggcacc
     7141 tgtttttggc tatccggggc gatggcataa ggcagaccgt gcagatagat gccattttca
     7201 cctaacgatt caccgtggtc agaaagataa accaggctgg tggtaaattt atcctgatgt
     7261 tctttaagca gattaatcgc tttatcaaca atatagtcga cgtaaaccag cgtgttgtcg
     7321 taagtgttca ccagttgctc tttggtacag gtctggatct cattggtgtc gcaggttggg
     7381 gtaaatttcc tgaactgcgg cggatagcgg ttgtaatagg tcggaccgtg gctgccgatg
     7441 gtgtgtaaga caatcacgcc atcaccctgc aggttattga tgtactcttc cagcccgtgg
     7501 aacagcactt cgtcatagca ttcgccgttg atgcactgat caggcagatt cagcgcggtg
     7561 acgttctggt gaggcacgcg gtcgcaggca cctttacagc cgccatcgtt gtcattccac
     7621 agcacgttga tgcccgctcg ctgaatgata tccagcacgc cttcctggtg ctgtgccagc
     7681 tcttctttgt agtgctcacg cggcatatcc gagaacatgc acggtactga aactgccgtt
     7741 gccgtgccgc aagatgcggt attagggaaa tagaccacgt tatctttcgc cagccgcggg
     7801 ttagtttcac gcgggtagcc gttgagggag aagttctccg cccgcgaggt ttcgccgaca
     7861 atcaggatgg tcaaattttt acgtttttcg ttctgcatta acgggttgcg gtgcgcgtct
     7921 tcaccaattc gcaccagcgg cagatttgcc agtcgctgat gggagtacca tgaccagctg
     7981 gcaacaatgc tgttagaggg gcttaaggat ttcaccagct ctttgttatt gcggaacaac
     8041 gaggcgtagt ctttataaaa cagtgcggcg accagcaaaa tcagcagtac agaaaccaga
     8101 atattggctc cacggaaaag aacactgcgc agacgcgagg tggcaggttt gatttttagc
     8161 cagcaggcaa tcagctcagc aagcacgccg ctgaatccca gcgttaataa catttgcggt
     8221 gtcatcagcg cataactttc tgctggagtg gtatcaataa tattggcaat catcgagcgg
     8281 tcgatgacga tgccgtaagt cattatgaaa tattgtgcag ccgcgccaat cagaataaac
     8341 aggcaggcca gtggtcgatt aagccataag aaagagctta gtgtcaggac aatattaatc
     8401 acgctgaaag cgacaaccgg catcgacaag aaaaccagta cgttatgcag cgaatccagc
     8461 ggcagcgcct gcaacacctg tttaaaaaag gcaatattca ggcagataga gatataaaaa
     8521 gcggccaaca atagccaggc gagtaaattc aaagagggtc tttttagtag gcgcttcaac
     8581 atcacggtgt ttccatcgaa caaagtgcgc atatgctcgc aaagcaaaat taagccaacc
     8641 ttaagttctt aaggttggct tttatgtttg ctggattaag gggaattaat ctttgcttat
     8701 tggtgcatct aagggatacg ggtttttatg tagccggttg taattcagcg catacatggc
     8761 ggtgatgacc atcagggtga caaatgacca cataacctct ttcgccccgg accccaccac
     8821 ggcccaaatg cagtagagga aggcaatggt agtaacagcc agatatgccg ggcgtgcttt
     8881 accaaagtga ccgtggccga gcagcagtaa cgccgcacag gtgtaaagat atggcaccag
     8941 tgtaaagatg accgagacgg aagaaaccag accgaactct ttggtcgcgt ttggtgaaat
     9001 gctgctgagc tggaagatgg tcatcaaaat accgacgata atcagccccg ccactggcgt
     9061 acccgcttta tttacacggg caaaaatcgg tgggaacagt ccgtcatcgg cagcggcttt
     9121 cgccgtttga cccgccagca acgtccagcc gcccagtgaa cctaagcaac ccgcagctgc
     9181 gcagaaggaa acaatggccc cagcggtgtc acccaacgcc atccgtgcgg catcaccgaa
     9241 tggcgaggca gaaacgcgca gtgcggcatt agggatcatc cccataatcg cggtggtgga
     9301 gagcacatag caaacggcgg caatcaatac cccaccaatg gtggcgatag ggacattgcg
     9361 tttcgggttt ttcaccacac ctgcggcaac ggaggcactt tccacaccga tgaacgacca
     9421 cagcgtaacg ttaagggtac tttgaatcgc accgaaggtg cccaggccgc tgacgttcca
     9481 tgccgccata taggtttcac cacggaacca gaaccagcca aatacggcaa tcccgacgat
     9541 ggggatcagc gccagcacag tggcaacggc ctgcacgcgg gtgatcattt tcggaccgac
     9601 aatgttcagc aggacgaaga tccacagcac cacgacgcag gtgatggtta ataccaatgg
     9661 atctttcaga atcgggaaga agtaacttaa atatcctacg ccaatgacca ccatggcgat
     9721 attgccgatc cagcaggcca gccagtagag gacgttggtt tgataaccga gaaacgggcc
     9781 aaagcagcgg cgggcgtaag cgtaagaacc accaggactt gggtcgagga acgacatttt
     9841 ggcgtatacc atcgagagcc ccagcgcacc gataatcgtc accaaccatc cataaatggc
     9901 aatcccgcca gtggaggcca ggtttgcagg taacagaaaa acacctgacc ccataatatt
     9961 ccccgacacc atcagggtga cggggattaa gcccactttg tgagcatcag catccgaaga
    10021 cataattaaa ctcctgcgaa ggcgagcttc gtgacaataa attacgtcat atcatacgcc
    10081 tgcattcgat agttgaatta ttatcgaccg ggttatcgct gatgccgtta ttaatatagc
    10141 tcttcgttaa ggttaaaaat caggcgacag tgcgttctct gtgctgacta acataatgca
    10201 gcggcgtcat accgtagaag tctttaaaga cagaaataaa gtacgacgta ctgttgtagc
    10261 cgcaggactg tgatacctga gagatatttt taccgtccat cattaattca tttacggcat
    10321 aacgcatgcg gcaggtggtg attatttggc tataactggt gttttcgctt ttcaactttt
    10381 tctttaacag acttgggcta agacataaac aactggctac catactaaga ttccagtctt
    10441 tgtgaatatc gctttcaata atttgataaa cgctgtcact tacacaatta cgtaacatat
    10501 acatcattaa tgaaaggaat tttttactgt cgagaaaacg ggatagcacc gtgaataata
    10561 atgcgcgtgt tcgttccttt tctgactcag tttctgcggg catcatgctg tgttgcgcgg
    10621 caacgcgaaa gacgtctggc gtcaggcatg gcagggtgag tatgggcgta gcgctacgtt
    10681 gccatactgg tatttgcgag agatctttat tcagaaaccg gaggtaatct ttgatgatgt
    10741 cgtgactaat atgagcaacc aaagtgttgt taagagagga gacgtcgata atattatttt
    10801 cacaatttaa taacgccata tggttagctt taaggctaat aggttctttc ccattcaccc
    10861 ttatccagac atctttttca gtcaataaaa caatacaagg ttggtcgctg caaatcctca
    10921 tacatgtact cctgagtgcg aataaaagac cagttctcgc taaagcaaag cgataccggg
    10981 aaatggttat ataattgcat gatgaatata aa
//
LOCUS       DAZOZX010000128        10859 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZOZX010000128 DAZOZX010000000
VERSION     DAZOZX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.128"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHUPT_003284"
     CDS             <1..794
                     /locus_tag="ACHUPT_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR0820726.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHUPT_003285"
     CDS             complement(744..1937)
                     /locus_tag="ACHUPT_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0820727.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHUPT_003286"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHUPT_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0820728.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHUPT_003287"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHUPT_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0820729.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHUPT_003288"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHUPT_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0820730.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHUPT_003289"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHUPT_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0820731.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHUPT_003290"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHUPT_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0820732.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACHUPT_003291"
     CDS             complement(10573..10716)
                     /locus_tag="ACHUPT_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820733.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2698 a         2745 c         2837 g         2579 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatatag
//
LOCUS       DAZOZX010000129        10829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZOZX010000129 DAZOZX010000000
VERSION     DAZOZX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.129"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(123..1967)
                     /gene="selB"
                     /locus_tag="ACHUPT_003292"
     CDS             complement(123..1967)
                     /gene="selB"
                     /locus_tag="ACHUPT_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0820734.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(1964..3355)
                     /gene="selA"
                     /locus_tag="ACHUPT_003293"
     CDS             complement(1964..3355)
                     /gene="selA"
                     /locus_tag="ACHUPT_003293"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0820735.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3453..4061)
                     /locus_tag="ACHUPT_003294"
     CDS             complement(3453..4061)
                     /locus_tag="ACHUPT_003294"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0820736.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(4132..5217)
                     /locus_tag="ACHUPT_003295"
     CDS             complement(4132..5217)
                     /locus_tag="ACHUPT_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0820737.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5220..5582)
                     /locus_tag="ACHUPT_003296"
     CDS             complement(5220..5582)
                     /locus_tag="ACHUPT_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0820738.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6119..8032
                     /gene="mtlA"
                     /locus_tag="ACHUPT_003297"
     CDS             6119..8032
                     /gene="mtlA"
                     /locus_tag="ACHUPT_003297"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0820739.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8127..9275
                     /gene="mtlD"
                     /locus_tag="ACHUPT_003298"
     CDS             8127..9275
                     /gene="mtlD"
                     /locus_tag="ACHUPT_003298"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0820740.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9275..9862
                     /gene="mtlR"
                     /locus_tag="ACHUPT_003299"
     CDS             9275..9862
                     /gene="mtlR"
                     /locus_tag="ACHUPT_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0820741.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9874..10083)
                     /gene="yibT"
                     /locus_tag="ACHUPT_003300"
     CDS             complement(9874..10083)
                     /gene="yibT"
                     /locus_tag="ACHUPT_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0820742.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10367..10729
                     /locus_tag="ACHUPT_003301"
     CDS             10367..10729
                     /locus_tag="ACHUPT_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0820743.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2630 a         2900 c         2763 g         2536 t
ORIGIN      
        1 attattatta attatcaaaa ttaatctaat aaaaagcaca ttgtattcat taaacaatgt
       61 gctttattta aaatcataag aaatttgtat taatttattt taaaaattaa ttaatcattt
      121 ccttattttt ccggaaataa taatgcgtcg cgcaataaat gatcatttcc acgacgacgc
      181 gtaaagccaa tgcggtcaaa atattccaga atctgaattg ccagctttcg gcctacgcct
      241 aagcgatcgc ggaaatccgc cgcgcaggtt gaaccacact cctgatcgag atcgcggatc
      301 atattggcaa attcgacaat ccgatcgtta cggtaataac gatctttgac gatcgcggta
      361 attattcctt gctgcgccgc ctggcgtagc gtcaggcgca ttgcctgctc gtcggtaccc
      421 gtctcttttg ccagatcacg cacccaccac ggctcgtcac caaacagcgg ctctgctttt
      481 tgccagatgg cctgctgctc ttcgctgaag cccgctttgt gatccggcag atgcagccaa
      541 ccgtgatgac tgaggatgtc gccgctttcg cgcatctttt caatcagcaa cagtactagc
      601 gcttcatctt ccattggcaa cgccatacgt cgcagacgtt cgcgcccagg cccaggttca
      661 tcgcgatgtt gctcatgata agtcgctaat gtgtcgagaa ttttccgttg ccagcgagcg
      721 gcaaccggcg cattcaacaa gctataacca gcctgaatat agccaggctg ttgcagcagt
      781 tcgcgcatcc cttcgccgtt gagctggcgc gcccaagcga aatcaggaag attaaccgcg
      841 ccgcgttgca ggtgaacggc taacgcttcg ctatcattct gtgccctggc gagggaagcc
      901 agccattgca gatactctgg tttacgttta ccgcgacgcg gcgggttaag catcacgacg
      961 cgcgctccgg ccagcgtgtt gcgggcagag atatcgcgca ataccaggcg gtcgttatct
     1021 gccagccata acggggtgtc gaagaccagt tcagcaaggt tatcttccag cagtgaaacg
     1081 cgtcccgtga cgtggctggc ggcgtggtga atatgcagcg gctgccactg ggtcagcggt
     1141 gcatgggttt gaagctcgac aatcacccgt gtgaacggct ctggcggcac atcggcaagc
     1201 agccagtcgc cacggttaat ctgctctttt tccgcatcac ccgcgatgtc aagcgcgata
     1261 cgctgcccgg cattggcggt ttctgttggc tggttttgcg catgcagcgc acgtacacgc
     1321 atcggtttat ttacaccagt cagccagagt gaatcgccta ccttcacttc cccgcttaac
     1381 gccgtaccgg tgacgaccag cccggcacct tttacggtaa atgcgcggtc aatcgcgagg
     1441 cggaaactat gttggctggc gtgctcgcgt tccggcaact gaagcagatg ctcgcgcagg
     1501 gcatcaattc cccgaccttc ggttgctgcg gtgataaaca gttttgcctc agcaaaaccg
     1561 tattcccgca gaacctcctt tacctggcgt tcaacctcat caacacgcgc ttcgtccacg
     1621 cgatcggctt tggtcagcgc cactgtcagc atcgggttac cggtcagctg caaaatcgcc
     1681 agatgctcac gggtctgtgc catcacgccg tcatcgcacg ccaccaccaa cagcgcgtga
     1741 tcgataccac caacgcccgc cagcatgttg gaaagaaact tttcatgacc gggaacgtcg
     1801 ataaaaccag gcacgcgacc atccggctgc ggccagtagg catagccgag atcgatggtc
     1861 atgccgcgct ttttttcttc cggcagacgg tcagcattta cgccagtaat cgcctgcaat
     1921 aaggttgttt tgccgtggtc aacgtgtccg gcagtcgcaa taatcatttc aacaacatct
     1981 ccaaaaaccg ttgctcatct tcaaggcagc gtaaatccag ccacaatcgt ccgtcataaa
     2041 tacgaccaat caccggcact ggcaattcac gccagcgggc ggctaatgac tcaaggtggc
     2101 taccgcgtcc atcatggggt gtaaacgtta atgccgcgct cggcaggcga tcaaccggca
     2161 gcgaaccact gccaatctgc gaaagacatg gcataacctg taccgcaaac tccgcgccgt
     2221 aatgtgcgac aaggggagcc tgtaaacgtt gtgcctggat ttgaatgacc tctgcgctgc
     2281 gggtaagcag gcgcagggtc ggtaattttt cactcagagc ttcagggtgt aaataaagac
     2341 gcaacgtggc ttccagcgcc gcgagggtca ttttatccgc gcgtaatgca cgcttcagcg
     2401 ggtggctttg caggcgggcg atcatctctt ttttaccaac gataatcccg gcctgcggcc
     2461 cgcctaacaa cttgtcgccg gagaagctca ccagactgac gcccgccgca atcaactcct
     2521 gcggcattgg ctctttcggc aaaccgtact ggctaagatc gaccagcgag ccactgccta
     2581 aatcagtcac cactggaaca tccagctctt tgccgagcgc caccagttca gcttcgtcta
     2641 tcgctttggt gaacccctga atgctgtagt tactggtatg tactttcatc aacagtgcgg
     2701 tattttcatt caccgcctga cgataatcat tcgcgtgcgt gcggttggtg gtccctactt
     2761 cgtgtagggt gcagcctgcc tgacgcataa catcgggaat acgaaacgcg ccgccaatct
     2821 ccaccagttc gccgcgagat accaccacct ctttcccgct ggcagtggcc gccaacatca
     2881 ataacaccgc cgccgcattg ttattgacga tacaggcatc ttccgccccc gtaatacggc
     2941 acagcagctg cgccagcgcc cgatcgcgat gtccgcgtcc ggcgtcgtcc agatcgtact
     3001 cgagggtcac tggcgaacgc atagcctgcg caacggcttc caccgcggct tccgcctgta
     3061 aagctcgccc aaggttggta tgcagcacgg ttcccgtcag gttgatcacc ggacgcagcg
     3121 cgctctgcgc ctctttcgtc aaccgggcat cgacttcttg cgcccagttt tcacaccacg
     3181 caggcagcgt ctggctgcca cgaatcactt ctcgcgcttc gtcgagcatc tgacgcaaca
     3241 attccaccac gcgggtgtga ccataagtat cacgcaaaga aaggaaggag ctatcgcgca
     3301 ataagcgatc aatagccgga agttgactat agagggaacg cgtttcggtt gtcataggaa
     3361 acctggctga tcaaggccct ctcacacgga gaagggcgtt taacataacc acggattgta
     3421 acgtgagatg ggtcaggagg acatatcgcg catcaagcct ttggcggttc ggtgcgagca
     3481 aaactttcgc ggctaaacag gttttcgacc agtttgacta agtgtgggcg gtcgacacac
     3541 caacctggcg caacgcggcg gaaattgaga tatccgacag cacaggcaat ggcgatagtc
     3601 gccagattga ccgtatcggt tttgagtgtg ccatcgacca gatatccttc cagcacatcc
     3661 agactgcggt tgattttctc ccgctggcgt aacaattcat cttcagactg ctgcgccgcc
     3721 ggacgcgcct gttcacgcac cgataccagc ccggcatcca taatgccatc cgccagcgcc
     3781 tcaattttgc gcacccgcaa cgactccagc ggatcgcgcg gcaacatcgc cggagcgaca
     3841 ttcattaact caatatattc agcgatgatc ggcgaatcaa accagcattc gccctcttcg
     3901 gtcaccagca ccggcacttt ccctaacggg ttaaattgcg ccacgccgtt atccgcgtta
     3961 tagggcagtt cattaataaa ttcgaaagtt atgccctttt ctaacaacag aataaaaagt
     4021 ttgcgtacaa acgggctggt gtagctaccg acgagtttca tgccgagtcc tttgtgcgag
     4081 gaaaaatatc agtatggctc aggctcacgc aaaacacagg tgatataagt ctcaatgatc
     4141 caaataaaga taatgcatcc aactggtcat tcttagcagc actttccgca tcaccgaaac
     4201 atggctgaaa tggtcggagt aaaccgccac ctgggcgtag atgccgtcgg gtaaggcatc
     4261 gatatcatcg ttagggtcca gttcaatggt tcccagcaca ccgtccgtgc cgggaacgac
     4321 cgttaatgat tgcaataccc cctgcgcctg ataagaaccg cctggcacga caggtaaaat
     4381 actagtcaat ttgccgtgga acacctgccc aggtagcgcg ttaaacacca cttccgcatc
     4441 atcgccaggt ttcagacgta acagcgagtt ttgccgaaat tgggcgacaa tttgccgttt
     4501 ttgctcgggg atgaagacca tcaccggacg cagcggcaag gcagctgcgt atgtacctgg
     4561 gcggatcagt acctgagtga cgtagccatt gcttggtgcg cgaatgacag tctgctcaag
     4621 attatatttt gcttcagtaa gttgcgctct taagctcaca atctgcgatt gctcgccgtt
     4681 gaccatactg tcgagctgac tctggatctg cgcctgctcc gccaccgagc ctttcactaa
     4741 cgcatcctgc gcgaggaaat tttgccgcgc atcgtcgatg tcacgttccg agaacggatt
     4801 caccgccgcc tggctgcctt tcaggtaacg ttgataattt ttaaacagac ggtcgcgctc
     4861 cgctgaaacc tgggtggtgt tggcctgcgc ttcagtgagc tgcgcacgca gcgtctttat
     4921 attatgcgtc gccgtcatca ggtcagcctg aagtctgtca actcgcgcct ggtaacgaac
     4981 cgggtcgagc ttaaaaagca cctcgccctt ttgaataagc tgattattct tgtcagtgac
     5041 ttcagtaaca attcccgtca cctgtggcgt gatagggatc gctatcactg ccttttgcgc
     5101 ggtaaaagtg taagggtggt tgtagttcat caacaaaatc aaaccactca ccagaaacac
     5161 gcctcccagc gccgccgtcg ccagcgccac gtaagttaaa acaatcaata gatccataat
     5221 tagatctccg ggaacgtgga atagtcagtc tttttctcgg cggcgagttg atgctccagc
     5281 cgggaaattc ggtcagaaag tgcggcgatt tccggttcag tcgctttctc ctgcgacgca
     5341 acatgtgact gcatacccca gccacgctcc ggttgataga gcgtcgccca gatccacaga
     5401 aacggccaga taacatgcag agtaaacagg ctcacccagc ccgccgtatg aatagcgtcg
     5461 gcatgggggt gattgcgctt tttggcaatc agataaggga tgtcatgtat cgcgatgatt
     5521 ccataaaaaa tcaccagaaa gacgaagatc agcactccca acgcgaaata gtttagaaac
     5581 atatctgcct cggattcacg tttatcagtg ttgttttttg gctggcagcc agaagggagt
     5641 caggctgata ttttgacaat aatccgggtt cgcgattctc gccataacac caaagaataa
     5701 tttttagagg tgatgagttg cttagttaca taacgattgt atgacgaagg cataacatgc
     5761 tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg tgattcagat cacaaagatt
     5821 caacaaacca tcaaaacaaa aatgtgacat tactcacatt taaatgccat ttttagcgaa
     5881 aatcaccgcc ttgtttcttt tttatacagg cgttttgtga tgagcgtcac gtcaattacc
     5941 tctctacccc ctatatttat gtgactgata tcacacaaaa gcccgtcgac tggacagtta
     6001 accgattcag tgccagattt cgcagtatct acaaggtccg gctacctctg ccgccacatt
     6061 aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg gtgtttttat
     6121 gtcatccgat attaagatca aagtgcaaag ctttggtcgt ttcctcagca acatggtgat
     6181 gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta ttccaacagg
     6241 gtggttaccg aacgagacgc tggcgaagct ggtcgggccg atgatcactt atctcctacc
     6301 gctgctgatc ggttataccg gtggtaagct ggtaggcggc aaacgtggtg gcgtagtcgg
     6361 tgccattacc accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc tcggttctat
     6421 gattgcaggt ccgctgggcg gctggtgcat taagcacttc gaccgctggg tagacggtaa
     6481 gatcaaatcc ggttttgaga tgctggtgaa taacttctcc gcaggcatca tcgggatgat
     6541 cctcgctatt ctggcattcc tcggcattgg cccgattgtt gaagccctgt ccaaaatgct
     6601 ggctgcgggc gttaacttca tggttgtcca tgacatgctg ccgctggcgt ctatctttgt
     6661 tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac cacggtatct tctcgccgct
     6721 gggtattcag cagtcccatg aactgggtaa atcaatcttc ttcctgattg aagctaaccc
     6781 aggtccaggt atgggcgtgc tgctggcgta catgttcttt ggtcgtggta gcgctaaaca
     6841 gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt atccacgaaa tctacttccc
     6901 gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc ctcggcggta tgactggcgt
     6961 gttcacgctg actatcctgg gcggtggtct ggtttctccg gcatctccgg gttctatcct
     7021 tgctgtactg gcgatgacac caaaaggtgc ttatttcgct aacatcgcgg gtgtgtgtgc
     7081 ggcgatggct gtctccttcg ttgtctctgc tattttgctg aaaaccagca aagtgaaaga
     7141 agaagatgat attgaagcag caactcgtcg tatgcaggac atgaaagctg agtctaaagg
     7201 cacatctccg ctatctgcgg gcgatgtgac taacgacctg agccacgtac gtaaaatcat
     7261 cgttgcctgt gacgccggta tgggttcaag tgcgatgggc gcaggcgtgc tgcgtaagaa
     7321 aattcaggat gcaggtctgt cgcagatttc ggtcactaac agcgcgatca acaacctgcc
     7381 gccagatgtg gacctcgtca tcactcaccg tgacctgacc gaacgcgcta tgcgccaggt
     7441 tccgcaggca cagcatattt cgctgaccaa cttcctcgac agcggcctgt acaccagcct
     7501 aaccgaacgt ctcgttgctg cccagcgcca tactgaaaac gaagtgaaag tgaaagacag
     7561 tctgaaagac agctttgacg attccagtgc taacctgttc aaactgggcg cggagaacat
     7621 cttcctcggt cgcaaagcag caaccaaaga agaagcgatt cgttttgctg gcgagcagct
     7681 ggtgaaaggc ggttacgttg agccggaata cgttcaggcg atgctggatc gtgaaaaact
     7741 gaccccgact tatctgggtg agtctatcgc ggtgccacac ggtacggttg aagcgaaaga
     7801 tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg gaaggcgtgc gcttcggtga
     7861 agaagaagat gacattgccc gtctggtgat tggtattgca gcccgtaaca acgagcacat
     7921 tcaggttatc accagcctga ccaatgcact ggatgatgag tccgtcatcg agcgtctggc
     7981 acacaccacc agcgtggatg aagtgctgga actgctggca ggtcgtaagt aatccaatcc
     8041 taccctctcc tcacggagag ggtttgggtg agggaaaagc ctcaccccag ccctctcggg
     8101 taaaaacatt gatgaaggtt aatactatga aagcattaca ttttggcgca ggtaatatcg
     8161 gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat ccaactgacg tttgccgatg
     8221 tcaatcaggt ggtacttgat gccctgaatg cccgtcatag ctatcaggtt catgtggtcg
     8281 gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc tgtcagcagc attggtgatg
     8341 atgtcgttga tctgattgct caggtcgatt tagtcactac cgccgttggc ccggttgtgc
     8401 tggaacgtat tgctccggct atcgccaaag ggctggtgaa acgcaaagaa caaggtaatg
     8461 aatccccgct gaacatcatc gcctgtgaaa acatggtacg cggcaccacg cagctgaaag
     8521 gtcatgtgat gaacgccctg ccggaagacg ccaaagcgtg ggtagaagag cacgttggct
     8581 ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc ttcggcaact aacgatccgc
     8641 tggaagtgac ggtagaaact ttcagcgaat ggattgtcga taaaacgcag ttcaaaggcg
     8701 cactgccgaa catcccaggc atggagttaa ccgacaacct gatggcattt gtcgaacgta
     8761 aactcttcac cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa ctggccggtc
     8821 atcagaccat tcgtgacgct attctcgacg agaaaatccg cgcggtggta aaaggtgcga
     8881 tggaagaaag tggtgcggta ctgatcaagc gctacggctt tgacgcagac aaacatgcgg
     8941 cgtacatcca gaaaatcctc ggtcgttttg agaacccgta tctgaaagat gatgtagagc
     9001 gcgtaggccg tcagccgctg cgtaaactga gtgctggcga ccgtctgatc aagccactgc
     9061 tcggtacgct ggaatacagc cttccgcaca agaatctgat tcaggggatt gctggtgcaa
     9121 tgcacttccg cagtgaagat gatccacagg ctcaggaact ggcagcactg atcgctgaca
     9181 aaggtccgca ggcggcgctg gcacagattt ccggtcttga tgccaacagc gaggttgtat
     9241 ccgaggcggt aaccgcttat aaagcaatgc aataatggtg gaccaggcgc aggacaccct
     9301 gcgcccgaat aacagattgt cagatatgca ggcaacaatg gaacaaaccc aggccgttga
     9361 aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc tgatcaccgc
     9421 cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt caggtattcc gcaaagacga
     9481 ttacgcggtg aagtatgctg tagaaccgtt actcgacggc gacggtccgc tgggcgatct
     9541 ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt aaccgccagg aatacgaaga
     9601 tgcggaactg ctgatggcat tgcgtgaaga gctaaatcac gacggcaacg agtacgcctt
     9661 taccgacgac gaaatccttg gaccctttgg tgaactgcac tgcgtggcgg cgttaccacc
     9721 gccgccacag tttgaaccag cagactccag tttgtatgca atgcaaattc agcgctatca
     9781 acaggctgtg cgatcaacaa tggtcctttc actgactgag ctgatttcca aaatcagctt
     9841 aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc ccctctacct gcttcggccg
     9901 ataaagccga cgataatact ccagacgttg tagatataac ggcacgcttt catcgggtat
     9961 tccggacgga atcgcgttac ggggaggaag ttttttcaga tactcccgga acgcctggct
    10021 tgatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac ctaatttgcc
    10081 catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat tttagtttag
    10141 gttcccggta aagatgacat taaggaaaac gtgctgaatc ctcaataatc atccgctgct
    10201 gtttattatc tttttctata gatttcttaa ttaatcaacg aattgcgtag tttttgagca
    10261 tgttaatgat tacgttgcag aactattaca atgtcatcag ttgtgaacaa agcacctggt
    10321 cgcgcatact aggggctata aatttatctt tatcagaagc catcacatga aagaagtcga
    10381 aaaaaacgaa atcaagcgtc tcagcgatcg cctggacgcc atccgccacc agcaggccga
    10441 tctgtcgctg gttgaagccg cagacaaata tgccgagctg gaaaaagaga aagccacgct
    10501 ggaagcagaa attgctcgcc tgcgtgaggt tcatagccaa aaactgagta aagaagcaca
    10561 gaagctgatg aagatgccgt tccagcgcgc aattaccaaa aaagagcagg ctgatatggg
    10621 caagctgaag aaaagtgttc gcggactggt ggttgtgcac ccaatgaccg cgctggggcg
    10681 cgaaatgggc ctggaagaga tgactggatt ttctaagact acgttttaag cccctcagac
    10741 gactggttcg atcgtatcaa cccgcatctg gcttgcctta gctggtcaat gcggggactc
    10801 tcctgtctac tcgcctaatt accaaacaa
//
LOCUS       DAZOZX010000130        10811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZOZX010000130 DAZOZX010000000
VERSION     DAZOZX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.130"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            107..1006
                     /gene="lysO"
                     /locus_tag="ACHUPT_003302"
     CDS             107..1006
                     /gene="lysO"
                     /locus_tag="ACHUPT_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0820744.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1150..2802
                     /gene="hcp"
                     /locus_tag="ACHUPT_003303"
                     /gene_synonym="priS"
     CDS             1150..2802
                     /gene="hcp"
                     /locus_tag="ACHUPT_003303"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0820745.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2814..3782
                     /gene="hcr"
                     /locus_tag="ACHUPT_003304"
     CDS             2814..3782
                     /gene="hcr"
                     /locus_tag="ACHUPT_003304"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0820746.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3915..5633
                     /gene="poxB"
                     /locus_tag="ACHUPT_003305"
     CDS             3915..5633
                     /gene="poxB"
                     /locus_tag="ACHUPT_003305"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0820747.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5670..6671
                     /gene="ltaE"
                     /locus_tag="ACHUPT_003306"
     CDS             5670..6671
                     /gene="ltaE"
                     /locus_tag="ACHUPT_003306"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0820748.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6682..8112
                     /locus_tag="ACHUPT_003307"
     CDS             6682..8112
                     /locus_tag="ACHUPT_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0820749.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8211..9224
                     /locus_tag="ACHUPT_003308"
     CDS             8211..9224
                     /locus_tag="ACHUPT_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0820750.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9221..10051)
                     /gene="amiD"
                     /locus_tag="ACHUPT_003309"
     CDS             complement(9221..10051)
                     /gene="amiD"
                     /locus_tag="ACHUPT_003309"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0820751.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10048..10371)
                     /locus_tag="ACHUPT_003310"
     CDS             complement(10048..10371)
                     /locus_tag="ACHUPT_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0820752.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10582..>10811)
                     /locus_tag="ACHUPT_003311"
     CDS             complement(10582..>10811)
                     /locus_tag="ACHUPT_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0820753.1"
                     /translation="KNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAA
                     DQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2457 a         2869 c         2863 g         2622 t
ORIGIN      
        1 ctctgatgca taacatttcc ttttaattgc aactttactc gcccttccgc tttcagtagt
       61 gtgtcatcgc gcatttcgac ttcttctttg caaggactgg attttcatgt tttctgggct
      121 gttaatcatt ctggttcccc tgattgtggg ttacctcatt ccgcttcgcc aacaagctgc
      181 gttaaaagct attaatcagc tattaagctg gatggtttac cttattctct tttttatggg
      241 tatcagtctg gcgtttctcg ataacctcgc cagtaacctg ttggcgattc tgcattattc
      301 tgccgtcagt attaccgtta ttttactgtg taatattgcc gccctgatgt ggctggagcg
      361 aggcctgccg tggcgcaacc accatcagca agaaaaactc ccgtcgcgta ttgcgatggc
      421 gctggagtcg ctaaaactgt gcggcgtagt agtgattggt tttgccattg gtctgagtgg
      481 actggctttc ttacaacacg cgaccgaagc cagtgaatac acgttaattt tgctactttt
      541 cctcgttggt attcagttgc gcaataatgg catgacctta aagcagattg tccttaatcg
      601 ccggggaatg attgtcgccg tggtggtggt tgtcagttca ttaattggtg gtttaattaa
      661 cgcctttatt cttgatctcc ccatcaatac cgcgctggca atggcctccg gtttcggctg
      721 gtattctctt tccggtattt tattgaccga atcttttggt ccggtaatcg ggagcgcggc
      781 gttttttaat gatctggccc gtgaactgat tgctattatg ttgatccctg ggctgattcg
      841 ccgcagccgc tctactgcac tgggcttatg cggtgccaca tcaatggatt tcaccctgcc
      901 cgttcttcaa cgtactggcg ggctggatat ggtcccggcg gcaattgttc acggttttat
      961 tcttagcctg ttagtgccga tcctcatcgc ctttttctct gcgtaatacc tctctggcgg
     1021 tagttccctg ccgccaaaat tgcgctaaat caatctccct taaagttgca tgaaaaatcc
     1081 cttttatccc cgcgttaagc gtcttaccct taaacatgta tattaaatat aactttaaaa
     1141 ggtgtgatca tgttttgtgt gcaatgtgaa caaactatcc gtactccggc agaaaacggc
     1201 tgctcatacg cgcaggggat gtgtggtaaa acggcggaaa cttctgacct tcaggattta
     1261 ctcatcgcgg cgctgcaagg gctttctgcc tgggcggtaa aagcgcgtga atacggcatc
     1321 atcaaccacg atgttgacag cttcgcgcca cgtgcctttt tctcaaccct gaccaacgtt
     1381 aacttcgatt ctccgcgtat tgtcggctac gctcgtgaag cgattgcctt gcgcgaggcg
     1441 ctgaaagcac aatgcctggc tgtagatgcc aacgcgcgcg tcgataaccc gatggctgac
     1501 ctgcaactgg tgagcgatga tctcggcgag ctgcaacgtc aggcagcaga atttacccct
     1561 aacaaagata aagcggcgat tggcgaaaac attctcggcc tgcgtctgct gtgcctgtat
     1621 ggcctgaaag gtgcggcggc ctatatggaa cacgcgcatg ttctcggtca atacgacaac
     1681 gatatttatg cccagtacca taaaatcatg gcgtggctgg ggacctggcc tgccgatatg
     1741 aacgcgcttc ttgagtgttc aatggaaatc ggccagatga acttcaaagt gatgagcatt
     1801 ctggatgcag gcgaaaccgg taaatacggt cacccgacgc caacccaggt caacgtcaaa
     1861 gcgacggcgg gtaaatgcat tctgatttcc ggtcacgatc tcaaagatct ctacaacctg
     1921 ctggaacaga ccgaaggcac gggcgttaat gtctacaccc acggcgaaat gctgcctgcg
     1981 catggctacc cggagctgcg taaattcaag catctggtcg gtaactacgg cagcggctgg
     2041 cagaatcagc aagtggagtt cgctcgtttc cctggcccca tcgtgatgac ctcgaactgc
     2101 atcatcgacc caaccgtagg cgcttatgac gatcgtatct ggacccgcag catcgttggc
     2161 tggcctggcg tgcgtcatct ggatggcgaa gatttctctg cggtcatcgc ccaggcgcaa
     2221 caaatggcgg gcttcccgta cagcgaaatt cctcacctga tcaccgtggg ctttggtcgc
     2281 cagacgctgc ttggtgcagc cgatacgctg attgatctgg ttagccgtga aaaactgcgt
     2341 catatcttcc tgcttggtgg ctgtgacggc gcacgcggcg agcgccacta cttcaccgat
     2401 ttcgccacca gcgtgccgga tgactgcctg atcctgaccc tcgcctgtgg taaatatcgc
     2461 tttaacaaac ttgagtttgg cgatatcgaa ggtctgccgc gtctggtaga tgcaggtcaa
     2521 tgtaacgatg cttactcagc aattattctg gctgtcactc tggcagagaa actgggctgt
     2581 ggtgtgaacg atctgccgct gtcgctggtg ctctcctggt ttgaacagaa agcgattgtc
     2641 attctgctga cgctgctttc tctgggcgtg aaaaatatcg tcaccggccc gactgcacct
     2701 ggtttcctga caccagacct gctggcggtg ctgaacgaga aattcggcct gcgttctatc
     2761 accactgttg aagaagacat gaagcaactg ttgaacgcgt aaggagtttg tcgatgacga
     2821 tgccaacgaa tcaatgcccg tggcggatac aggttcatca cattacgcaa gaaacgccgg
     2881 atgtgtggac gatttccctg atttgccacg atcactaccc atatcgcgcc gggcaatatg
     2941 cactggtcag cgtgcgaaac tcagcggaaa cgctgcgtgc ttacaccatt tcctccacgc
     3001 caggcgtgag tgaatatatc accctgaccg tgcggcggat tgatgacggt gtcggctccc
     3061 agtggctgac gcgcgatgta aaacgcggtg attatctctg gctttcggac gcgatggggg
     3121 aatttacctg cgacgataaa gcagaagata aattcctgtt gctggcggca ggctgcggcg
     3181 tcacgccgat tatgtcgatg cgtcgctggc ttgcgaagaa ccgtccacag gccgatgtgc
     3241 gggtgatcta caacgtgcgt acgccgcagg atgttatttt cgccgatgag tggcgtaact
     3301 atccggtaac gctggtggca gaaaataacg ttaccgaagg ctttatcgct ggtcgcctca
     3361 ctcgcgaact gctggcagat gtacctgact tagcttcacg taccgtgatg acctgcggcc
     3421 cggctccgta tatggattgg gtagagcagg aagtgaaagc gctcggcgtg acgcgtttct
     3481 ttaaagagaa attcttcacc ccagtagcag aagcggcgac cagcggtctg aaattcacca
     3541 aactgcaacc ggcacgagaa ttttacgccc cggttggcac cacgctactg gaggcgctgg
     3601 aaagcaataa cgttccggtt gtcgctgcct gccgtgcggg tgtttgcggc tgctgtaaga
     3661 caaaagtggt ttccggtgaa tatacggtga gcagtacaat gacgctgacc gacgccgaaa
     3721 tcgctgaagg ttacgtactg gcctgctcct gccatccgca gggggatttg gttctcgcat
     3781 aatcgcctta tgcccgatga tattcctttc atcgggctat ttaaccgtta gtgcctcctt
     3841 tctctcccat cccttccccc tccgtcagat gaactaaact tgttaccgtt atcacattca
     3901 ggagatggag aaccatgaaa caaacggttg cagcttatat cgccaaaaca ctcgaatcgg
     3961 caggcgtgaa acgcatctgg ggagtcacgg gcgactctct gaacggtctt agcgacagtc
     4021 ttaatcgcat gggcaccatc gagtggatgt ccacccgcca tgaagaagtg gcggcgtttg
     4081 ccgctggcgc tgaagcacaa ctttcaggag aactggcggt ctgtgccgga tcgtgcggcc
     4141 caggcaacct gcacttgatc aacggcctat tcgattgcca ccgcaataac gttccggtac
     4201 tggcgattgc cgctcatatt ccctccagcg aaattggcag cggctatttc caggaaaccc
     4261 acccacaaga gctattccgc gaatgtagtc actattgcga gctggtttcc agcccggagc
     4321 agatcccaca agtgctggca attgctatgc gcaaagcggt gcttaaccgt ggcgtttccg
     4381 ttgttgtatt accgggcgac gtggcgttaa aacctgcgcc agaaggggca accacgcact
     4441 ggtatcatgc gccacagcca gtcgtgatgc cggaagaaga agagttacgc aaactggcgc
     4501 aactgctgcg ttattccagc aatatcgccc tgatgtgtgg cagcggctgc gcgggggcgc
     4561 ataaagagtt agttgagttt gccgggaaaa ttaaagcacc tattgttcat gccctacgcg
     4621 gtaaagagca tgtcgaatac gataatccgt atgatgttgg aatgaccggg ttaatcggat
     4681 tctcgtcagg tttccatacc atgatgaacg ccgacacgtt agtgctactc ggtactcagt
     4741 tcccctaccg tgcgttctac ccgaccgatg ccaaaattat tcagattgat attaatccag
     4801 ccagcatcgg cgcgcatagc aaggtagata tggcgctggt cggcgatatc aaatcaaccc
     4861 tgcgcgcatt gctgccactg gtggaagaaa aaaccgatcg caagtttctg gataaagcgc
     4921 tggaagatta ccgcgatgcc cgcaaagggc tggacgattt agctaaaccg agcgagaaag
     4981 ccattcaccc gcaatatctg gcgcagcaaa ttagtcattt tgccgccgat gacgccatct
     5041 ttacctgtga cgtcggcacg ccaacggtat gggcggcacg ttatctgaaa atgaacggca
     5101 agcgtcgcct gttaggttcg tttaaccacg gttcgatggc taacgccatg ccgcaggcgt
     5161 tgggtgcgca ggcaaccgag ccggaacgtc aggtggtcgc catgtgcggc gatggcggtt
     5221 ttagcatgtt gatgggcgat ttcctctcag tagtgcagat gaaactgccg gtgaaaatta
     5281 tcgtctttaa taacagcgtg ctgggctttg tggcgatgga gatgaaggcc ggaggttacc
     5341 tgacagacgg tactgagctg cacgacacca actttgcccg cattgccgaa gcgtgcggca
     5401 ttacgggtat ccgtgtagaa aaagcctctg aagttgatga agccctgcaa cgcgccttct
     5461 ctatcgacgg tccggtgttg gtggatgtgg tggtcgccaa agaagaatta gccattccac
     5521 cgcagatcaa actcgaacag gccaaaggtt ttagcctgta tatgttgcgc gcaattatca
     5581 gcggacgcgg cgatgaagtg atcgaactgg cgaaaacgaa ctggctaagg taaaaagggt
     5641 ggcatttccc gtcataataa ggacatgcca tgattgattt acgcagtgat accgttaccc
     5701 gaccgagccg cgccatgctc gaagcgatga tggccgcccc ggttggggac gacgtttacg
     5761 gagacgaccc taccgttaat gctctgcagg actatgcagc agagctttcc ggtaaagaag
     5821 ccgccatttt tctgcctacc ggcactcagg ccaacctggt cgctctgctc agtcactgcg
     5881 aacgtggcga agagtatatt gtcggtcagg ccgcgcataa ctatctgttt gaagccggtg
     5941 gcgcagcggt gctgggcagt attcagccgc aacccatcga cgcggctgcc gacggcacgc
     6001 taccgctgga taaagtggcg atgaaaatca aacccgacga tatccatttc gcccgcacca
     6061 aattactcag tctggaaaac acccacaacg gcaaagtgct gccgcgtgaa tacctgaaag
     6121 aagcatggga atttacccgc gagcgcaatc tggcgctgca tgttgacggt gcgcgcatct
     6181 ttaatgccgt ggtggcttac ggctgcgaac tgaaagagat cacgcaatat tgtgattcgt
     6241 tcaccatttg cctgtcgaaa ggtcttggga cgccagtcgg ttcattactc gtcggtaatc
     6301 gtgattacat taaacgtgcc atccgctggc ggaaaatggc cggtggcggg atgcgtcagt
     6361 ccgggattct ggctgccgcc gggatgtatg ccctggaaaa taacgtcgca cggttgcagg
     6421 aagatcacga caacgccgcc tggatggcgg agcaactgcg tgaagcaggc gcggatgtga
     6481 tgcgtcagga cactaatatg ctgtttgttc gcgtcgggga agaaaatgct gccgcgttag
     6541 gcgaatacat gaaagcgaga aacgtgctga ttaacgcctc gccgattgtc cgcctggtga
     6601 cgcatcttga cgtctcgcgc gaacaactgg cggaagtcgc cgcccactgg cgcgcattcc
     6661 tggcgcgtta aggagagaga cgtgccgcaa cgcattttag ttctcggtgc cagtggctac
     6721 attggtcagc atctggtgcg cacactcagc cagcaagggc atcagatcct agcggcggca
     6781 cgtcatgtcg acaggcttgc aaagctgcaa ctggcaaacg tcagttgcca taaagtcgat
     6841 ctcagctggc cggataacct tccggccctg ttgcaggata tcgataccgt ctattttctg
     6901 gtgcacagca tgggcgaagg cggcgatttt atcgctcagg agcgccagct ggctctcaac
     6961 gtccgcgatg cgctacgtga agtaccagtt aagcaattaa tctttctcag ttcgttgcag
     7021 gccccgccac atgagcagtc ggatcatctg cgagctcgtc aggctacggc ggacattctt
     7081 cgtgaagcga atgtaccagt gaccgaactt cgggccggaa ttatcgttgg cgcaggttca
     7141 gcggcgttcg aagtcatgcg cgatatggtc tacaacctgc cagtgttaac gccgccacgc
     7201 tgggtacgtt cacgcaccac gcccatcgcg ctggaaaact tgctgcacta tctggtggcg
     7261 ctgttagacc atccagccaa cgaacaccgc atcttcgaag ccgccggacc agaggtgctc
     7321 agttatcagc aacagtttga acattttatg gcggtgagcg gtaagcgccg ctggttgatc
     7381 cccatccccc tccccacccg ctggatttcg gtgtggtttc tcaatgtgat tacttccgta
     7441 ccgcccacca ccgccagggc gttgattcag gggctgaaac acgatctgct ggcagatgat
     7501 accgcgctac gtgcactcat cccacaacgg ctgatcgctt tcgatgacgc ggtacgtagc
     7561 acgttgaaag aggaagaaaa actggtcaac tccagcgact ggggctacga cgctcaggcc
     7621 tttgcccgct ggcgaccgga gtacggttat ttcgccaaac aggcggggtt taccgttaaa
     7681 acgtccgcca gccttgcggc tttatggctg gtggtgaacc aaatcggcgg taaagagcgt
     7741 tatttctttg gcaatatttt gtggcagaca cgggcgttga tggaccgcgc gatcggtcat
     7801 aatctggcga aaggccgccc ggagcgcgaa tatttacaga ccggcgatgc ggtggatagc
     7861 tggaaagtga ttgtcgttga accgaaaaag caacttacgt tgttatttgg catgaaagcg
     7921 ccggggctgg gacgactgtg ttttaccctg gaagataaag gtgactatcg tactatcgat
     7981 gtccgcgctt tctggcatcc gcacggtatg ccggggctgt tttactggtt attgatgatc
     8041 cccgcgcatc tgtttatttt tcgcggaatg gcaaaacaaa tcgccagact ggcagaacaa
     8101 agcacagatt aatcgacaaa acctgcgtta ttctttcatg tttcccattg catcatgggg
     8161 gaaatcacgg aagaatgcgc acgtcatttt ctcaacaaca gtggattgat atgaaggtac
     8221 tggttaccgg cgccaccagc ggcttaggtc gaaacgcggt agagttttta tgccagaaag
     8281 gcatcagcgt gcgtgcgagc ggtcgcaacg aagcaatggg taaattgctg gaaaaaatgg
     8341 gcgcagagtt tgttccgacg gatctgaccg agctggtttc atcacaagct aaagtgatgc
     8401 tcgcgggcat tgatacgctg tggcactgct ccagctttac ctcaccctgg gggacacaac
     8461 aggctttcga tctggctaac gttcgcgcca ctcgccgcct gggtgaatgg gctgtcgcct
     8521 ggggtgtgcg taactttatt catatctctt ccccctccct gtacttcgat tatcaccacc
     8581 atcgcaatat taaagaagat tttcgccctc accgcttcgc caacgagttt gcccgcagca
     8641 aagcagccag cgaagaagtg atcaatatgc tttcgcaggc gaatccacaa acgcgcttta
     8701 ctattctgcg cccacaaagt ctgttcggac cgcacgataa agtctttatt ccccgtctgg
     8761 cgcatatgat gcaccactac ggcagtattc tgttaccgca tggcggcagt gcgctggtcg
     8821 atatgaccta ctatgaaaat gccgtgcacg caatgtggct ggcaagccag gaagcctgcg
     8881 ataagctacc ttccgggcgt gtgtacaaca tcaccaacgg cgagcatcgc acactgcgca
     8941 gcatcgtgca gaagctgatc gacgagttga atattgactg tcgtattcgt tccgtccctt
     9001 acccgatgct ggatatgatc gcccgcagca tggagcgttt aggccgcaag tcagcaaaag
     9061 agccgccgct gacccactac ggcgcctcca agttgaattt tgactttacg ctggatatta
     9121 cgcgtgcgca ggaagagtta ggttatcagc cggtcatcac cctggatgaa ggcatcgaga
     9181 aaactgccgc ctggctacgc gaccacggaa aactgccgcg ctaatcctgc ccgtatttct
     9241 ccagcaatgc ttcggcaatc gcctgagttt ctgcatccgc ttcgccgtta tataacgtcg
     9301 ggcggaaatg catctggaat gccataatca cgcgccgctg ctcgcgcggt gtcatatcag
     9361 gttttacgtc ataaccgtag cgcgccaaaa gctccagcaa tgacgcggta tctaccggtg
     9421 tgtgcggcgc gcgcccggca aggtaaaagt tcacccgctg cgcatccggc caggcaccaa
     9481 tcccctgctg cgccagttgc tgccagggaa ataatggccc cggatcgtct ttgcgctgcg
     9541 gtgcgatatc cgcatgtgcc actacgtttt ccggcttgat gtgataacgg gcaataatat
     9601 ctttcgccag cggaataagc gcctgaatct gtgcctgttc aaacggggca aaatatttca
     9661 ctccggatga tttttgccag ccacggtttt ccagctcaat gccaatagag gtgtcgttaa
     9721 ggcgcgttgc cccgcgccag gcgctaatcc ccgcatgcca ggccagttct tgttccggca
     9781 ccagttgcca gatgcgcggt ttaccgttgt atcgcggtgg caccgcaggg atcagataat
     9841 gcgagctgac ctgcttatca gtcagtgtcg ccagcgagct atcaaaatca tctgcggtgt
     9901 agtggatcac cagcacttta atgcgcggat acgccgcctg cgcctggtgt cgggtatcaa
     9961 gctgatatcc ctctttctcg acaatgcctt tttcgcctgc acaccctgcc agtaacagag
    10021 cggcagcgac cagccagaag actcttctca tcgacgcgtt ttcaccgccg taccgctgac
    10081 gctaaccatc agcatactgc cgttttgccc gaccgtttcg tagtcgatat caataccaac
    10141 tacggcatcg gcccccagcg cccgcgcctg ggagcctaat tcctcaaagg cgatctcccg
    10201 tgctttacgc agttcttttt catacgcacc ggaacgtccg ccaacgatat cgcggatacc
    10261 ggcaaagaaa tcacggaaaa tattggcacc taaaatcgct tcgccggtca ccacaccgca
    10321 atattcaacg atggtcaggc cttccagagt tggggttgta gaaaattgca tgatcgatct
    10381 ccttgtttgt taagccttcg atctctaaag cattatcgga ctgatatgcc atgttgggaa
    10441 gaaggttttg atcagtttag gaaatcatct cattgtgtca aaaacaccca cttaagtctt
    10501 tacgattatc cgttgcataa cccgatattg gtttcattat agggtaccgc ttacctgtct
    10561 tattatgttg caccaacaac attaatgagc ggaaagaggc aacttatatt gtaaatgact
    10621 cctaaagtct tccccatctt gtgactgagg tctataccgc tggattgctt tttggtcagc
    10681 cgccgcaaaa caagaaagta ataagatctc aatgcttttg tcattaatat aagcgaagat
    10741 cattcttgca aactcgtata taaacgcccg atcctgagcg cttaatagtt taatacaata
    10801 tttattttta a
//
LOCUS       DAZOZX010000131        10522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZOZX010000131 DAZOZX010000000
VERSION     DAZOZX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.131"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..2104
                     /locus_tag="ACHUPT_003312"
     CDS             <1..2104
                     /locus_tag="ACHUPT_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0820754.1"
                     /translation="YPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKA
                     AQSSITAPDRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVA
                     YAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEII
                     TPKKPGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYW
                     FHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYP
                     SADIRAKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDN
                     ISKDEWKNLHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSK
                     HEQIERSITPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYER
                     DDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNL
                     FNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLN
                     KGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVII
                     NKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPR
                     RSALLLGKTGEPPNYLYL"
     gene            2440..3372
                     /gene="glaH"
                     /locus_tag="ACHUPT_003313"
     CDS             2440..3372
                     /gene="glaH"
                     /locus_tag="ACHUPT_003313"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0820755.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3392..4660
                     /gene="lhgO"
                     /locus_tag="ACHUPT_003314"
     CDS             3392..4660
                     /gene="lhgO"
                     /locus_tag="ACHUPT_003314"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0820756.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4683..6131
                     /gene="gabD"
                     /locus_tag="ACHUPT_003315"
                     /pseudo
     CDS             4683..6131
                     /gene="gabD"
                     /locus_tag="ACHUPT_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6139..7425
                     /gene="gabT"
                     /locus_tag="ACHUPT_003316"
                     /pseudo
     CDS             6139..7425
                     /gene="gabT"
                     /locus_tag="ACHUPT_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7663..9063
                     /gene="gabP"
                     /locus_tag="ACHUPT_003317"
     CDS             7663..9063
                     /gene="gabP"
                     /locus_tag="ACHUPT_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0820757.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9084..9746
                     /gene="csiR"
                     /locus_tag="ACHUPT_003318"
     CDS             9084..9746
                     /gene="csiR"
                     /locus_tag="ACHUPT_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0820758.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9747..10196)
                     /gene="kbp"
                     /locus_tag="ACHUPT_003319"
     CDS             complement(9747..10196)
                     /gene="kbp"
                     /locus_tag="ACHUPT_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0820759.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10280..10438)
                     /locus_tag="ACHUPT_003320"
     CDS             complement(10280..10438)
                     /locus_tag="ACHUPT_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0820760.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2644 a         2620 c         2725 g         2533 t
ORIGIN      
        1 ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag aaaaagtcat
       61 taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa cttaccgaga
      121 gctgaataaa gctgcacaga gtagcattac agcgccagac aggataggag aagggaaaac
      181 atgggtttat caacgagata caatggttga aagaggtgat aacagtggtg ttcatcagta
      241 tggtccagct gaacatttca ctcacattat atctgacaaa ccgtccccaa aagataagta
      301 tgttgcatat gctattaaca ttcctgacta tgagctagca gccgatgtat ataatattaa
      361 cgtgacgtca ccttccggac agcaagaaac atttaaaata ctaatcaatc cagaacatct
      421 acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat
      481 cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat
      541 cagggaaaat atgtctgagt ttgcacgttc ccattatggg tatatacaaa tccctccagt
      601 gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt actggtttca
      661 cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggggatt tgcaggattt
      721 tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt
      781 taactttatg ggaatcgggg gaagtggtaa aaatgacctg gattatccct ctgctgatat
      841 acgagcgaag atcagtaaag atatagaaag tggtatccct ggctattggc aaggtcaggt
      901 tttgattcca ttcattaaag atccagtaac aaaagaacgt aaacaaatcc atccagaaga
      961 tatacatctc actgcaaaag acttcgaagc aagtaaagat aacatctcta aggatgaatg
     1021 gaaaaacctc catgcattaa aagaaaagag tttaaatgga atgcctaaaa caacccccaa
     1081 aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt
     1141 acgaggttta cgttatgatg cagcaaaaca ctcaaaacat gaacaaatag aaagatcaat
     1201 aaccccaccg cttaaaaatt ataatgagcg attacacaat actaacttat ttaacccaaa
     1261 atatcatgaa aaaaccgtta tgaattacat ggaatatctg gtaacttgtc agttgaatga
     1321 agaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg atttttcatt
     1381 gctcatgaag acgataaaag ccttttcatt cgggggagat ctcctaaccc ttgcatcaaa
     1441 accgggttcc acaatctcaa gcatcccatc agaaagacgg atattgatta acattaacca
     1501 cgattttcct aacaatggca atcttttcaa tgactttcta tttaaccatc aacaagatga
     1561 acaattagca atggcatata tggccgctct cccgttcagc aggcctttag tttactggga
     1621 tggccaggta ttaaaatcaa cgactgaaat taaaaattat gatgggccca cgcgtgtcgg
     1681 tggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca atgaattcca
     1741 cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat ttgcaactaa
     1801 aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc ctcatgatgg
     1861 actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta acaaattgca
     1921 acctggaata tataaaaaca tggggagtaa tagcgttaac attattatta ataatacccg
     1981 aaaaattatc cccccttgta aagtatttac gcttagaggc ggatctctaa atatcaatat
     2041 tcctaggcgt agcgctcttc ttttagggaa aacaggagaa ccgccgaact atctctattt
     2101 ataatttaat gttatatctg ccccgataaa acggggcaga taatatgttt agtttactaa
     2161 cggtcatttt gtagtgaagc catttactgt tttttatcga ccagataatc tgttctctaa
     2221 tgttaactcc ccctaacctg ttgctttagt tattcatttc ctgtctcact ttgccttaat
     2281 accctacgtt aaatgttact aatttgttgc ttttgatcac aataagaaag caatatgtcg
     2341 cttttgtgcg catttttcag aaatgtagat atttttagat tatggctacg aaatgagcat
     2401 cgccatgtca ccctacatct cataagagga tcgcttctga tgaatgcact gaccgccgta
     2461 caaaataacg ctgtcgattc aggccaggac tatagcggat tcactctcat cccgtcggcg
     2521 caatccccgc gtctgctgga actcaccttc accgaacaga cgaccaaaca gtttctcgaa
     2581 caggttgccg aatggcccgt gcaggcgctg gagtacaaat cgtttctgcg ttttcgggta
     2641 ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt gaagaccctg
     2701 ctaaaccgtg ctgaaggtgc gctgttgatc aatgcggtag gtatcgatga tgtggcgcag
     2761 gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg ctcaaatttc
     2821 gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga taactcagac
     2881 agctatctgc gtcagccgca ccgcgtgatg gaactgcaca acgacggcac gtatgtcgaa
     2941 gagatcaccg attacgtgct gatgatgaaa atcgatgact gggaacatct ggaccactat
     3001 ttccgccatc cactggcgcg tcgcccgatg cgctttgccg cgccgccgag caaaaacgtc
     3061 agcaaagatg ttttccatcc agtgttcgac gttgatcaac agggtcgccc ggtgatgcgc
     3121 tatatcgacc agttcgtcca gccaaaagat tttgaagaag gcgtgtggtt gagcgagctt
     3181 tccgatgcca ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt tggcaaattc
     3241 ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc gctttactcc gcacccggat
     3301 ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca ccactaccag
     3361 acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta ttggcggcgg
     3421 catcatcggc atgtcgaccg ccatgcaact gattgatgtt tatccggatg cccgcattgc
     3481 gttgctggaa aaagagtccg gcccggcctg tcaccagacg ggccacaaca gcggcgtgat
     3541 ccatgccggg gtctattaca cgcccggcag cctgaaggcg cagttttgcc tggcggggaa
     3601 ccgtgccacc aaagcctttt gcgatcaaaa cggcattcgc tatgacaact gcggcaagat
     3661 gctggtcgcc acgtctcagc tcgaaatgga acggatgcgc gccttatggg aacgcacagc
     3721 ggcgaacggt atcgagcgcg agtggataaa cgccgtggaa ctgcgcgagc gcgaaccgaa
     3781 tatcaccggg cttggtggca tttttgtgcc gtccagcggc attgtcagct accgcgaagt
     3841 gacggcggca atggcaaaaa tcttccaggc cagaggcggc gagattattt ataacgccga
     3901 agtcagcgcc ctcagtgagc ataaaaacgg cgtggtgata cgcacccgtc agggcggtga
     3961 atatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc tggtgaaaat
     4021 gctcggcctc gaaccgggct ttattatctg cccgttccgt ggcgagtatt tccgccttgc
     4081 gccggagcat aaccagattg ttaaccacct gatttacccc attcccgacc ccgcgatgcc
     4141 atttttgggc gtgcatctca cccgcatgat cgacggcagc gtgaccgtcg ggccaaacgc
     4201 ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac ttctcattta gtgacacgct
     4261 ggagattttg agctcgtcgg ggattcgccg ggtgctgcaa aaccatctac gctcaggact
     4321 gggcgagatg aaaaactcgc tgtgcaaaag cggctatctg cggctggtgc aaaagtattg
     4381 tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg cgcaggcagt
     4441 atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccac gcacgatcca
     4501 cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg cgcatattgt
     4561 cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc tgcgagcggc
     4621 acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa caggacgtaa
     4681 caatgaaact taacgacagt aacttattcc gccagcaggc gttgattaac ggggaatggc
     4741 tggacgccaa caatggtgaa gtcatcgacg tcaccaatcc ggcgaacggc gacaagctgg
     4801 gtagcgtgcc gaaaatgggc gcagatgaaa cccgcgccgc tatcgacgcc gccaaccgcg
     4861 ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc caccattctg cgcaactggt
     4921 tcaatttgat gatgcagcat caggacgatt tagcgcgcct gatgaccctc gaacagggta
     4981 aaccgctggc tgaagcgaaa ggcgaaatca gctacgccgc ctccttcatt gagtggtttg
     5041 ctgaagaagg caaacgtatt tatggcgaca ccattcctgg tcatcaggcc gataagcgcc
     5101 tgattgttat caagcagccg attggcgtca ccgccgccat cacgccgtgg aactttccgg
     5161 cggcgatgat tacccgtaaa gccggtccgg cgctggcggc aggctgcacc atggtgctga
     5221 agcccgccag tcagacgccg ttctctgcgc tggcgctggc ggaactggcg attcgcgcgg
     5281 gcattccggc tggcgtgttt aacgtggtca ccggttcggc aggcgtggtc ggtaatgaac
     5341 tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ttagaccgag attggccgcc
     5401 agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg ggcggcaacg
     5461 cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc gcgctggcct
     5521 cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac gtgcaggacg
     5581 gcgtgtatga ccgctttgcc gaaaaattac agcaggcggg gagcaaactg cacatcggcg
     5641 acgggctgga gaaaggcgtc accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag
     5701 tggaagagca tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag
     5761 cgcacgaacg cggcggcaac ttcttccagc caaccattct ggtggacgtt ccggccaacg
     5821 ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc actgttccgt tttaaagatg
     5881 aagccgatgt gattgcacaa gccaatgaca ccgagtttgg tcttgccgcc tatttctacg
     5941 cccgtgattt aagccgcgtc ttccgcgtcg gcgaggcgct ggagtacggc atcgtcggca
     6001 tcaataccgg cattatttcc aatgaagtgg ccccattcgg cggcatcaaa gcctcgggtc
     6061 tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa tatatgtgca
     6121 tcggtcttta actggagaat gcgaaggaac agcaataaag agttaatgca gcgccgcagt
     6181 caggcgattc cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac
     6241 tgccgggtgt gggacgttta aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg
     6301 ctcaataccg ggcacctgca tccgaaggtg gtggccgcgg tggaagcgca gttgaaaaaa
     6361 ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct gtgcgagatt
     6421 atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc tgctggttac gaccggttcc
     6481 gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc
     6541 gcttttagcg gcgagtatca cgggcgcacg cattacacgc tggcgctgac cggcaaggtg
     6601 aatccgtact ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc gctttatcct
     6661 tgcccgctgc acggcataag cgaggatgac gctatcgcca gcatccaccg gatcttcaaa
     6721 aatgatgccg cgccggaaga tatcgccgcc atcatgattg agccggttca gggcgaaggc
     6781 ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac gcgctctgtg tgacgagcac
     6841 gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt
     6901 gcgatggagc agatgggcgt ggcgccggat cttaccacct ttgcgaaatc gatcgcaggc
     6961 ggtttcccgc tggcgggcgt caccgggcgc gcggaagtga tggacgctgt tgcaccgggc
     7021 ggtctgggcg gcacctatgc cggtaacccg attgcctgcg tggctgcgct ggaagtgttg
     7081 aaggtgttcg agcaggaaaa tctgctgcaa aaagccaacg atctggggca gaagctaaaa
     7141 gacggattgt tggcgatagc cgaaaaacac ccggagatcg gcgacgtacg cgggctggga
     7201 tcgatgattg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc
     7261 accgccgaga tcgtggcacg cgcccgcgat aagggcctga ttcttctctc ctgcggcccg
     7321 tattacaacg tgctgcgcat ccttgtacca ctcaccattg aagacgctca gatccgtcag
     7381 ggtctggaga ttatcggtca gtgttttgat gaggcgaagc agtagcaccg tccccatgca
     7441 ggaggcgacg ctgcgcgtct tgtccggcct acgagaatcc aagtcggata aggcgtttac
     7501 gccgcatccg acaatctgta cgtgaacagg aataaatcta tgtcggccgg ataaggcgga
     7561 gccgctctcc ggcaaaaaga atcaataaca attaaacgcg tgacccgacg cgggaaatgt
     7621 cggggcgctc tcccaagtga cacactttcg agaggattca ggatggggca atcatcgcaa
     7681 ccacacgagt taggcggcgg gctgaaatca cgccacgtca ccatgttgtc tattgccggt
     7741 gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca tcgccgaagc ggacccggcg
     7801 gtattactgg cctatctgtt cgccggatta ctggtggtta tgattatgcg gatgctggcg
     7861 gaaatggcag ttgccacgcc cgataccggt tcgttttcca cctatgccga taaagccatt
     7921 ggccgctggg cgggctatac catcggctgg ttgtactggt ggttttgggt actggttatc
     7981 ccactggaag ccaacatcgc cgccatgatc ctgcactcgt gggttccagg cattcccatc
     8041 tggttatttt ccctcgtcat taccctcgcc ttaactggca gtaatttatt aagcgttaaa
     8101 aactacggcg aatttgagtt ctggctggcg ctgtgcaaag tcatcgctat cctggccttt
     8161 attttccttg gtgcagtcgc aattagcggt ttttaccctt atgccgaagt gagcgggatc
     8221 tcaagattgt gggatagcgg cggctttatg cccaacggtt tcggtgcggt attaagcgcg
     8281 atgttgatca ccatgttctc gtttatgggc gcagaaattg tcaccattgc cgccgcggaa
     8341 tccgacatgc cggaaaaaca tattgtccgc gccaccaact cggttatctg gcgtatttct
     8401 atcttctatt tgtgctctat ttttgtcgtc gtggcattaa taccgtggaa tatgccgggg
     8461 ctgaaagccg ttggatctta tcgctcggtt ctggaattgc tcaatattcc ccatgcgaaa
     8521 ttaatcatgg actgtgtgat attactttcc gtaaccagtt gcctgaactc ggccctgtat
     8581 accgcctcaa ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt
     8641 aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg cgcagcattc
     8701 ttaacggtgg tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc
     8761 tccggcgcta tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgc
     8821 aaaattctgc gagcagaagg aagcgaaatt cgcttgcgaa tgtggcttga tccctggctc
     8881 acctggctgg tcatcggctt tattaccttt gtgttggtag tgatgctatt ccgtccggcg
     8941 caacagttag aagtgatctc caccggctta ttagcgatag ggattatctg taccgtgcca
     9001 attatggctc gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc
     9061 tgataatgat caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga
     9121 agaacgatat tattcgcggt aattttcaac cggatgaaaa attacggatg agtttactga
     9181 catcgcgtta tgcacttggc gttgggccgt tgcaggaagc actttctcaa ctggtggcgg
     9241 aacggctggt cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg
     9301 agctgctcga tattttcgac gcccgcgcca atatggaagc gatgctggtc agtctggcga
     9361 ttgcccgcgg tggcgatgaa tgggaggcag acgttctcgc aaaagcgcat ctgctcagta
     9421 agctggaagc ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg
     9481 cgtttcatac agcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac
     9541 ggttgtttga tctggcggcg cgttatcgat ttatctggct gcggcgaacg gtgctttcgg
     9601 tggaaatgct ggaggataaa cacgatcagc accagaccct gactgcggcg gtactgccgc
     9661 gagatacagc gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta
     9721 tccagcaggc gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga
     9781 taaattttat ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga
     9841 ttagcgttac cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta
     9901 taaaactggc tggcagtggc tggtgtcgcc gttttcacct gatcatcaac actggcaata
     9961 ccggaaatat tccccaccgc aacaaggatt ttctccttcg cctcctgact caggccgtca
    10021 ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca ctttatcggc atcaggtata
    10081 ccggttttgc tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct
    10141 gtaaccgcat cccagagttt ttctccggca tctttaacaa aattgaacag acccatagct
    10201 acctcattcg ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc
    10261 cgcgggggtg agcacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg
    10321 ggatataccc aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac
    10381 cgagcagcac gccgatcggc ggcagaatga tggtgatgac gattctccag aaacccatat
    10441 gtactcccta taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt
    10501 aattttagcg gagcctgcca gt
//
LOCUS       DAZOZX010000132        10464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZOZX010000132 DAZOZX010000000
VERSION     DAZOZX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.132"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="ACHUPT_003321"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="ACHUPT_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0820761.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="ACHUPT_003322"
     CDS             213..401
                     /locus_tag="ACHUPT_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0820762.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="ACHUPT_003323"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="ACHUPT_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0820763.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="ACHUPT_003324"
     CDS             596..769
                     /locus_tag="ACHUPT_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0820764.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="ACHUPT_003325"
     CDS             complement(818..1891)
                     /locus_tag="ACHUPT_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR0820765.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHUPT_003326"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHUPT_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="ACHUPT_003327"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="ACHUPT_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0820766.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHUPT_003328"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHUPT_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0820767.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHELSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="ACHUPT_003329"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="ACHUPT_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0820768.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..10279
                     /gene="torA"
                     /locus_tag="ACHUPT_003330"
                     /pseudo
     CDS             7739..10279
                     /gene="torA"
                     /locus_tag="ACHUPT_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10276..>10464
                     /gene="torD"
                     /locus_tag="ACHUPT_003331"
     CDS             10276..>10464
                     /gene="torD"
                     /locus_tag="ACHUPT_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0820769.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2686 c         2789 g         2466 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagttcatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    10021 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt cgcagctggc
    10141 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    10201 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    10261 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    10321 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    10381 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    10441 gatctggaaa acagtattgc cacg
//
LOCUS       DAZOZX010000133        10080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZOZX010000133 DAZOZX010000000
VERSION     DAZOZX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.133"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHUPT_003332"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHUPT_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0820770.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHUPT_003333"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHUPT_003333"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR0820771.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHUPT_003334"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHUPT_003334"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0820772.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHUPT_003335"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHUPT_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0820773.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHUPT_003336"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHUPT_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0820774.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHUPT_003337"
     CDS             4025..4615
                     /locus_tag="ACHUPT_003337"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0820775.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHUPT_003338"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHUPT_003338"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0820776.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHUPT_003339"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHUPT_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0820777.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHUPT_003340"
     CDS             complement(6551..6658)
                     /locus_tag="ACHUPT_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR0820778.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHUPT_003341"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHUPT_003341"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0820779.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHUPT_003342"
     CDS             7648..7875
                     /locus_tag="ACHUPT_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0820780.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHUPT_003343"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHUPT_003343"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0820781.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..>10080)
                     /locus_tag="ACHUPT_003344"
     CDS             complement(9447..>10080)
                     /locus_tag="ACHUPT_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820782.1"
                     /translation="DGYCLLRAILVLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIE
                     ALVDTAFYNLREDVKTLFGVDLQSDNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCIL
                     HLPEFIFNDNKNLLALDTDTSDRIKAVKNFLAVLSDSICSLFIVNSNVASISLGNESF
                     STDEDLEYGYLTNTGNHYDVYLPPELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         2327 a         2609 c         2616 g         2528 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
//
LOCUS       DAZOZX010000134         9859 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZOZX010000134 DAZOZX010000000
VERSION     DAZOZX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.134"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(192..965)
                     /gene="uxuR"
                     /locus_tag="ACHUPT_003345"
     CDS             complement(192..965)
                     /gene="uxuR"
                     /locus_tag="ACHUPT_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0820783.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1180..2640)
                     /locus_tag="ACHUPT_003346"
     CDS             complement(1180..2640)
                     /locus_tag="ACHUPT_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0820784.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(2721..3905)
                     /gene="uxuA"
                     /locus_tag="ACHUPT_003347"
     CDS             complement(2721..3905)
                     /gene="uxuA"
                     /locus_tag="ACHUPT_003347"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0820785.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            4245..5588
                     /gene="gntP"
                     /locus_tag="ACHUPT_003348"
     CDS             4245..5588
                     /gene="gntP"
                     /locus_tag="ACHUPT_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0820786.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5839..6741)
                     /gene="fimH"
                     /locus_tag="ACHUPT_003349"
     CDS             complement(5839..6741)
                     /gene="fimH"
                     /locus_tag="ACHUPT_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0820787.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6761..7204)
                     /locus_tag="ACHUPT_003350"
     CDS             complement(6761..7204)
                     /locus_tag="ACHUPT_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0820788.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(7277..7807)
                     /gene="fimF"
                     /locus_tag="ACHUPT_003351"
     CDS             complement(7277..7807)
                     /gene="fimF"
                     /locus_tag="ACHUPT_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR0820789.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7817..>9859)
                     /gene="fimD"
                     /locus_tag="ACHUPT_003352"
     CDS             complement(7817..>9859)
                     /gene="fimD"
                     /locus_tag="ACHUPT_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR0820790.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2285 a         2600 c         2484 g         2490 t
ORIGIN      
        1 ccagaactgt ttgaattaaa aaatggtgaa tggaattata ttggtaaata gccactgaga
       61 agaatgagta ctaatattca ggttatacag atatttaaac aaaaccctgc aatttacaaa
      121 gtaatactca ttgcgacttc ttaatttaag aaagcgcaag gaacgtttac ccttgcgctt
      181 attataataa gtcaggcgtc gactttatcc agcggccatg aatcaaagag atagccgtca
      241 aaataaatat cgtcaacgtt cgagaattcc agcagacgtt gcttaacgtt ttccagatgc
      301 tgccacattg ccagcttcgc cgctcgggca tcttttttga ttaacgcggc gaggatctgt
      361 ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat ccagatggct gtgcaactgg
      421 atccacattg gattgttttc ccgccactgc caggactgac ggaataactc caccagcatg
      481 ctgttatgcg ttgcttcagc aatggcgaga tggaactgca tgtcaccgct ttcgctgcta
      541 cccggcgcgc tggaagccag ttcacgctct tccagttgca atgcctgacg cattttgacg
      601 atatcttcgc gggtagcctg cagtgcggca aactcggcga tgttactttc cagtaactga
      661 cgcgcctgta acagctcaaa aggacccgca tcattgcaga cgttggcatc cggactgtct
      721 gtgttgtggc tgcctgagct atcaagcaca tagataccgg caccccggcg cacttccacc
      781 agccctttga tctccagcat gatcagcgct tcacgtacca ccgtccgcgt gacatcaagc
      841 atttctgcaa tttcacgctc cggcggcagc cgttcgccag gattgtacgg cgtctttatg
      901 atcagatcgt ggatcatcgc cccgacttcc tggtaaggtc tttgcgcaga ggtgacagat
      961 ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt ttgccggatg cggcgtgaac
     1021 gccttatccg gcctacaacg ggcaatttta cggcccccgt taatctcaaa ctataccatt
     1081 agcactggaa gttggcacgt acaggtgggc aaaaaaacga aaactggtca accaaatatc
     1141 agtttgattg accagtaagt ctgtagggta atcagttagt tacagcgcag ccacacactc
     1201 gcgcgcaccg cgttcgcaca gctgctgata tgccgacgtc accgcgccaa caaagtcggc
     1261 attctgcggc agatcatcgg caaaaatacc gctcaggccc agcagcgctt tcacgcggtc
     1321 tgcgccctga tactgcgcgt taatcttctg gaactcggcc agcatcgggt cgaccacgtc
     1381 aatggcatta ccctgctcat ccacgccctg ggtgtaaccc atccagccag ccacgcccag
     1441 cgccaggtga cgccagctgc cgccgttttg caggtgcaga cgcaccgggt ccagtagacg
     1501 ctgcggtaac ttctggctgc cgtccatcgc aatctgccag gtacggtgac gcagagacgg
     1561 gttgctgaaa cgctcgatca gcagcgtcgc ataggcgttc aggtccgtac cttccggcat
     1621 cgacagcgtt ggcgcttgtt cctgcatcat cagggcaaag gccgctttgc gataatccgg
     1681 gttagtcatg gtgtcggcaa tggtttcata gccgccgagg taaccgaggt acgccaggaa
     1741 agagtggctg ccgttcagca tacgcagctt catcatttcg aacggcacaa cgtctgcaac
     1801 gaactgtgtg cccactttat cccagtccgg gcgaccatta acgaagttat cttcaatcac
     1861 ccactgacgg aacggttcgc aggcaatggc gcacgggtcg taaacaccca gctggtcagc
     1921 aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg cggtcaacca tggtgcacgg
     1981 gaaggtgacg ttctcttcaa tccatgccgc tagctgcggg tcacgcgcct gagccagccc
     2041 cagtaccgcg accttcgcca catggccgtt ttcacgcacg ttatcgcagg acatcaccgt
     2101 aaatgctttc agcccttttt cacgacgcag acgcaaggct tcgacgatgt aaccaatcgc
     2161 ggacttcggc gcagtcgggt tttccagatc gtgcttgatc agcgggttat tgagatccag
     2221 ttgaccgctt gccgcatcag cgcagtagcc tttttccgtg accgttagag agacaatcgc
     2281 cgtttgcgga cgcgccatcg cgttgagaat accttcgcag ccgtcgattt ccggatgcag
     2341 cgcttctttc atcgaaccga taattttcag ctcggtgctt tctgcgcctt tttccgctac
     2401 ggtgtacagc agttgctgtt ttttcaggtt ttcgatcagc atgcggtcgt tgcctggcat
     2461 caggttaact tcgcagatgc cccagtcgct gtcggtgctt tccagcagat gatgggtata
     2521 cagcgcctgg tgcgcgcggt gaaacgcccc gcaaccgaga tgcacaatgc gtgattccag
     2581 acgagaatga tcccatgacg ggcgggcaac cggcagattg ctgtcaacaa tagtagtcat
     2641 cgtaaactcc agaatccgtc ttcagagagt gacggcaagg aagaaggaac cggcacggcg
     2701 cgcagccatg ccggtgggta ttaacggata aagaaagcgc gctggatcgc cagttcgaca
     2761 ccgcgaactt cggccaggcc tttcagacga ccaattgcgg agtaaccagg gttggttttc
     2821 ttcttcaggt cgtccagcat ctgatgaccg tggtccggac gcatcgggat caggtcttct
     2881 ttgccttccg ctttacgacg gtgttcttct tcaacaatcg ctttcaccac tttgtacata
     2941 tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt tcgggttatc ttcacgcatg
     3001 gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact gcttgatcat atcaaccaga
     3061 tcgttgtcag cacgcacgcc gtaggaaccg gtgcacatgg tgaaaccgtt cgccatgctg
     3121 tttacggtat caaccatcca ctgcatatct tcaatggtgg aaacaatgcg cggcaagccg
     3181 aggatcgggc gcggcggatc gtccgggtga accgccatac gcacgccaac ttcttcagca
     3241 actggaataa tcgctttcag gaagacggca aagttttcgc gcagtttggc tttgtcgata
     3301 tctttgtaca gctccaggtg cttacggaac tggtcgaggg tatacccttc ttccgcgccc
     3361 ggcagaccgg caatgatgtt acgcgtcaga cgcgctttgt cttcgtcgct catagtggcg
     3421 aagcgttcag ctgcctgagc aatttcttct tcggtgtaat ccgcttccgc gcctggacgt
     3481 ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt cgaagcgcag agctttggag
     3541 ccgtctggca gcacgtattc gaggtcagta cgggtccagt cgagcaccgg catgaagttg
     3601 tagcacacgg tgcgaatgcc acactgcgcc aggttgcgca gagtctgctg atagttagcg
     3661 atccactgct catagttgcc agtgtgggtt ttgatatctt cgtgaattgg tacgctttct
     3721 acgacagacc acaccaggcc tgcgtcttca acgatcgcct tgcgtttgag gatctcttct
     3781 acggaccata cttcgccgtt cgggatatgg tgcagcgcgg taaccacgcc agttgcgccc
     3841 gcctgacgga catcagctaa agaaaccgga tcgtttgggc cgtaccagcg ccaggtctgt
     3901 tccatgattt ttcctctttc acatagtggg ttccacagca aagagacgcg gaaccttgga
     3961 gttgtttagg tatccggatg aacaaccaga ccgcagccgt ggcgttgcat cggatgagat
     4021 ggcgtataag ttctaccttt tggtaagaca tgtcaaccag aattctgaaa ttggttaacc
     4081 gcgtcgcaag aatttcactc tcatggttgt tgctgcaaat tgcagcaaag ggtaatcgat
     4141 ggttattgat cagaggatgt gacattcatc gcaacaatgg ttgaccaatt tacataacat
     4201 gtcgcccaaa ataacaacgg ttcaaccaag taaggtaaaa cgttatgcat gtgcttaaca
     4261 ttctctgggt ggtattcggc attggcctga tgctgatact gaatttgaag ttcaaaatca
     4321 attcaatggt ggctttgttg gtggcggcgc tgtccgtcgg gatgctggca ggcatggatt
     4381 tgatgtcgct gctgcacacc atgaaagcgg gcttcggcaa cacgctggga gaactggcta
     4441 tcatcgtggt gttcggtgcg gtcatcggta aattgatggt cgactccggc gcggctcacc
     4501 agatagcaca tacgctgctg gcgcgtctcg gtctgcgcta tgtacagctg tcggtgatta
     4561 tcatcggcct tattttcggt ctggcgatgt tctatgaagt ggcctttatc atgttagcgc
     4621 cgctggttat tgttattgcc gccgaagcta aaattccgtt cctgaaactg gcgatcccgg
     4681 cagtagcagc tgccactacc gcacattcac tgttcccacc gcagccgggt ccggtggcgc
     4741 tggtgaatgc ttatggcgcg gatatgggga tggtttatat ctatggcgta ctggtgacga
     4801 tcccaagtgt aatctgcgca ggtctgatcc tgccgaagtt cctcggcaat cttgagcgcc
     4861 caacgccatc attcctgaaa gcagatcaac cggtagatat gaataatctg ccctctttcg
     4921 gcgtttcgat tctggtgccg ctgatcccgg cgatcattat gatctctacc accatcgcca
     4981 atatctggct ggtaaaagat acccctgcct gggaagtggt taactttatt ggttcctcgc
     5041 cgattgcgat gtttattgcg atggtggttg cattcgtact ctttggcacc gcgcgtggtc
     5101 atgacatgca gtgggtgatg aacgcttttg aaagcgcggt gaagagtatt gcaatggtga
     5161 ttctgatcat cggtgcgggt ggcgtgctga agcagaccat catcgacacc ggcattggcg
     5221 acaccatcgg catgttgatg tcccacggca atatctcgcc ctacattatg gcatggctga
     5281 tcactgtgct aattcgtctg gcgacgggtc agggtgtcgt ttcggcgatg accgccgccg
     5341 ggattatcag tgctgcaatc cttgatccag caaccggtca gctggttggc gtgaatccgg
     5401 cgctgctggt actggcgacg gctgcgggtt ccaacaccct cacccacatt aatgatgcat
     5461 ctttctggtt gttcaaaggt tactttgacc tgtcagtaaa agacacgttg aaaacctggg
     5521 gtctgctgga gctggtcaac tccgtggttg ggctgattat tgtgctgatt attagcatgg
     5581 tagcgtaaaa gaacaccaga gcctgccaat ggcaggctca gactgatgac taaagtctaa
     5641 aaatgtgccc ggacagtccc ctcgcccctc cggggagagg gttagggtga ggggaacaga
     5701 ccagtaaagt gcgctttgtt tatgccagat gcgacgctga cgcgtcttat ctggcctaca
     5761 aagggctaac gtgcaggttt tgtaggtctg atagcgtagc gcctcaggta ccagcattag
     5821 caatgcgctg taattttttt attgataaac aaaagtcacg ccaataatcg attgcacatt
     5881 ccctgcagtc acctgccctc cggtacgtgc gtaatttgcc gttaatccca gacttaccgc
     5941 cgaagttcct actgctccta acgataccgt gttattcgct ggaataatcg taccgttgcg
     6001 cgtcaactgt acgccgacgc cctgcgctgg tgaaaacgac gcggtattgg tgaaaatcga
     6061 gttgcccgca tctgcggttg tgccggagag gtaatacccc aggttttggc ttttcgcaca
     6121 ataaacggta agaggaattg gcactgaacc agggtagtcc ggcagagtaa cggtgacatc
     6181 acgagcagaa acatcacaac cgccagtggg caccaccaca tcattattgg cgtaaatatt
     6241 ccacacaaac tgaaaatcat cgctgttata gttgttggtc tgtcgcaaaa taagcacggc
     6301 aattaatgaa ccagctttaa tagccacccc gcccgcactg ctcacaggcg tcaaataaag
     6361 cgccaccggc cacggcttat ccgttctcga attataaaca acccgcggcg tttcgctggt
     6421 ggtcggaaat ggatagctac tgccactata ttttacggtc ccggaaaaat tagataacac
     6481 gccgccgtaa gccgagcctc gttgcagtgt gacatagtct gtaatggttt ccggataatc
     6541 gttatggcaa aagatttgcg tcgaaagatc tacgaccagg ttttgcccca cattcacgac
     6601 aggcgcaagg tttacataaa cattagcgct gccaccgcca atagggatag cggtaccatt
     6661 ggcggtttta caggcgaatg accaggcatt taccgaccag cccatcagca gtacagcaaa
     6721 cagggtaata gctcgtttca ttacaattat ctcttcgggt tcagctgtag gtataggtga
     6781 tgctaatcac tgcctgaatg gttccctgag tggctccgcc atttaccgtc aatgctctga
     6841 cctgtaacgg gaagtgcgct gattgtgagg aatcatccac ctgaactgtt ttggttgcgc
     6901 cagtattcaa tgtgttgcca ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc
     6961 cctggttttt ataatatccg gtgctgtcgg ctgccccgct gaagctggca gtgaccctcg
     7021 acgttcccac cggacaatta gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc
     7081 cggcagacat aagactgaaa gaataaagat cgccgagatc taccgtggca ttggtggtgg
     7141 aaaccgtaca cggtttggcg acgaccttac cgttcaccgt gatggtgaca tcggctgcct
     7201 gtatcgtcgc acttgcgagc gccaacattg ccgccaatac atacccacgt ttgcaccatt
     7261 tcatgagcat ctccagttac tgatattcaa gagtgaaggt agccgtagca ttgatatgcc
     7321 ccgcagtgac aggcacctgt gtcgccatta gccgggcgta aaaattcagc gtatttggtt
     7381 tacccggcgt cagggtcgtc cacgaaatcg cagacgatgg agcattaagg ggtatttgat
     7441 tttgctgctc attcagaagc tgtattccca gtcccgcagc cgctgacacc gtattttcaa
     7501 gtgcaagcag gttggcattg tggctatctg caacgccggt aaacccaact tttacggcag
     7561 aaacggcatt accacagggt gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc
     7621 caatgttgtt aaattgcttc gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt
     7681 cagcggccac actacagccg ttatctctga catagccgcg gatagtaatc gtgctatccg
     7741 cagccaaaac gcgacttacc gccagccaca aaaaagcgca cagaagataa aaaggtttgt
     7801 ttctcatcac gcccccttaa cgacattcag ctgatagctg ggttaataac tgctgctgac
     7861 tctctggtgg cagttgataa ttggcgacac agtgagcatt ttcctcttct ccccatttca
     7921 cctgaacttt tcccgctaaa ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa
     7981 tgccgctact ctggctactc tctgatgtca ccatcgcccc aaacggcagc ggcttattat
     8041 tgtgagttag cgtcatgagc agttttatcc caacgcgcgc tttaaactct gctcgcacga
     8101 tcgccccacg agtgggaaca acgttagcga ccgcgttatc taaatcgacg ttatcagcca
     8161 aggtattggt atccagcgcc actctatttt cccgatattc agtggcataa ggcagcacgg
     8221 cataaccacg ccagtcggta cgcacccccg tctggttttc gacttttgca tcttttgcgc
     8281 caggcgcttt aacaagcacc accgtatcgt ttaacggctg ccccagcgtt acgccattgg
     8341 catgagccaa taccccaccg ctgactccgt aatagagctg cttaatatca tcgctatggc
     8401 tgtaaccgat attggcattg ccgtaaccac cgcgataatt cagcgtggcg tagcctgtac
     8461 ttccgctatt accatcgcct cccccggcat agccggtttg cacgctataa ctgaggttgt
     8521 tgtcttccag caacgtaccg tatacaccag ccagattggt catccgaccg ttgagatcgt
     8581 gtgacatgct gtagctggca ctggcatgtc gccactgaga tttactgtca gaacgcagcc
     8641 agtggctgaa aggaatattg acgttaagcg ctaacatctg atcccgtcct ttttgccagg
     8701 cgtttttcgt caggctatag ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc
     8761 cagcctggaa ttgctcatcg acattactcg ttccccaata agtttgatgg ctaccactca
     8821 aatacagtgt tgatgtgcgc ccgagttgct gagtaacggt gagctgtaat ttcccgcgtt
     8881 tgttataagc gaggttgtaa tagtcggtga atttcggctt aacctgaata actccatcct
     8941 gtgtttcgat gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc
     9001 cgctggtcga ataacggtaa cccactaact gaatattcgt gcctgattca ttgagcgatt
     9061 tgttatagag aaaacgcacc gattgtccgt catgctgact gtcatcggga agtgtggaat
     9121 tagcctgcgt catatccaca gacagagcgc ccagtgcctc catgtttttc ccgataccga
     9181 aattaaaagc acgataacga tccgccagtt gcgttccacc atatattgtc cagccagccg
     9241 gaaggccgtg gagtaatgta ctctggaaaa agcggggttt ttcctgctgc gcatttccac
     9301 tacggtattc tcctgccgta atggaataac gagtatgccc ttcacgttgc aaaagcggga
     9361 ctgacgaata gggtacggta aaaatctgcg tgctgccgtc agcctctttg atcgttacct
     9421 gcaagtcacc actattacct gcggcataga tatcgttgat ggtaaaaggc cccggtggca
     9481 ccgtactatt ataaatgcca tatccatttt gtttaatagt gacctgtgca gtaccacgag
     9541 caataccgtg gatcaccggg gcaaatcctc tttggctatc gggtaacata ttgtcatctg
     9601 aggccaattg tgcgccgcga aagttaatac catcgaaaat atcgccctgg gtataaccat
     9661 cacccaacgt cagccgggaa cgtaaaggaa ttatgtctcg ctcaatccag gtaatgatat
     9721 gctgccattt atttttgcta cctgatgaac tgtcgctact gttataactc caggtggtat
     9781 tgtcgcgtaa acgccacgca ccaatattta acccactctg taggtttaaa tatgcataat
     9841 ggctgttacc cccaatccg
//
LOCUS       DAZOZX010000135         9776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZOZX010000135 DAZOZX010000000
VERSION     DAZOZX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.135"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHUPT_003353"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHUPT_003353"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0820791.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUPT_003354"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUPT_003354"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0820792.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUPT_003355"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUPT_003355"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0820793.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUPT_003356"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUPT_003356"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0820794.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUPT_003357"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUPT_003357"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0820795.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHUPT_003358"
     CDS             complement(6031..6360)
                     /locus_tag="ACHUPT_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0820796.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUPT_003359"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUPT_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0820797.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHUPT_003360"
     CDS             7998..8171
                     /locus_tag="ACHUPT_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0820798.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUPT_003361"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUPT_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0820799.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2287 c         2433 g         2493 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atctct
//
LOCUS       DAZOZX010000136         9761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZOZX010000136 DAZOZX010000000
VERSION     DAZOZX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.136"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            383..1501
                     /locus_tag="ACHUPT_003362"
     CDS             383..1501
                     /locus_tag="ACHUPT_003362"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0820800.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHUPT_003363"
     CDS             1567..1815
                     /locus_tag="ACHUPT_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR0820801.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHUPT_003364"
     CDS             1880..2248
                     /locus_tag="ACHUPT_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0820802.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFCPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUPT_003365"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUPT_003365"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0820803.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUPT_003366"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUPT_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0820804.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUPT_003367"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUPT_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0820805.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUPT_003368"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUPT_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0820806.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUPT_003369"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUPT_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2387 a         2705 c         2367 g         2302 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gcctttttgc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg c
//
LOCUS       DAZOZX010000137         9680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZOZX010000137 DAZOZX010000000
VERSION     DAZOZX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.137"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(44..214)
                     /locus_tag="ACHUPT_003370"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHUPT_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUPT_003371"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUPT_003371"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0820807.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUPT_003372"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUPT_003372"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0820808.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUPT_003373"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUPT_003373"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0820809.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUPT_003374"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUPT_003374"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0820810.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUPT_003375"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUPT_003375"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0820811.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6241
                     /locus_tag="ACHUPT_003376"
                     /pseudo
     CDS             5109..6241
                     /locus_tag="ACHUPT_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="ACHUPT_003377"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="ACHUPT_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0820812.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHUPT_003378"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHUPT_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0820813.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHUPT_003379"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHUPT_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0820814.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHUPT_003380"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHUPT_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0820815.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHUPT_003381"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHUPT_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0820816.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..9423
                     /gene="epmB"
                     /locus_tag="ACHUPT_003382"
     CDS             8395..9423
                     /gene="epmB"
                     /locus_tag="ACHUPT_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0820817.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2479 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
     9421 taactgatta aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc
     9481 ctacatcgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
     9541 acgccgcatc cggcaagagc aacaaacgca agtacagcaa ccactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt
//
LOCUS       DAZOZX010000138         9572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZOZX010000138 DAZOZX010000000
VERSION     DAZOZX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.138"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..133
                     /locus_tag="ACHUPT_003383"
     CDS             <1..133
                     /locus_tag="ACHUPT_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0820818.1"
                     /translation="SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            230..382
                     /gene="hokE"
                     /locus_tag="ACHUPT_003384"
     CDS             230..382
                     /gene="hokE"
                     /locus_tag="ACHUPT_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0820819.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            733..885
                     /gene="hokE"
                     /locus_tag="ACHUPT_003385"
     CDS             733..885
                     /gene="hokE"
                     /locus_tag="ACHUPT_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0820820.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACHUPT_003386"
     CDS             complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACHUPT_003386"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0820821.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACHUPT_003387"
     CDS             complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACHUPT_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0820822.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4285..5487
                     /gene="fes"
                     /locus_tag="ACHUPT_003388"
     CDS             4285..5487
                     /gene="fes"
                     /locus_tag="ACHUPT_003388"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0820823.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACHUPT_003389"
     CDS             5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACHUPT_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0820824.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5705..9550
                     /gene="entF"
                     /locus_tag="ACHUPT_003390"
     CDS             5705..9550
                     /gene="entF"
                     /locus_tag="ACHUPT_003390"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0820825.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2105 a         2505 c         2630 g         2332 t
ORIGIN      
        1 cagtaatata agcgaactga aggatgctgt tacggaatat attgaatact ataacagcag
       61 aagaattagc ctgaaattaa aaggtctgac tccaattgaa tatcggaatc agacctatat
      121 gcctcgtgta taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa
      181 ccagacaatt tggatggtag tcccttcttc gcatggaggt aatataaaca tgctgacgaa
      241 atatgccctt gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggc
      301 cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct
      361 cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc
      421 aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg
      481 cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact
      541 gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt
      601 tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca
      661 atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga
      721 ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg
      781 gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt
      841 aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt gtgcggggag
      901 taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc
      961 ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
     1021 acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac
     1081 gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat
     1141 cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca agtgtcagcg
     1201 ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt
     1261 tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg
     1321 gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct
     1381 ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt
     1441 cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc
     1501 cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg
     1561 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
     1621 ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc
     1681 ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccggata
     1741 aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc tgacgttagc
     1801 atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
     1861 cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacctg
     1921 cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttcg
     1981 tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct ttggtttccg
     2041 ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg
     2101 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     2161 tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     2221 aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac gatccttcca
     2281 gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg ccgactgcgt
     2341 tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     2401 tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg ttgatgctgg
     2461 tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc
     2521 cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc ggcgctttat
     2581 aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca
     2641 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     2701 cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt
     2761 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc
     2821 cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac
     2881 ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2941 tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt
     3001 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca tactgcaccc
     3061 agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc
     3121 gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt
     3181 tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc agttccagcg
     3241 attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt
     3301 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     3361 tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg aagctgaatt
     3421 cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat
     3481 gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg
     3541 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     3601 gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca ccacgggtat
     3661 cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa
     3721 tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac
     3781 gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg
     3841 acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag
     3901 gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt gaaacaggtg
     3961 tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca
     4021 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     4081 cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt gatcctgaaa
     4141 gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     4201 tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca atgaaaaatt
     4261 gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     4321 cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc
     4381 tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac
     4441 atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca gcgaattgca
     4501 ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc
     4561 tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta
     4621 cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa tccacaaagc
     4681 tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     4741 ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat ctggaaaagt
     4801 gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt aacagcagaa
     4861 gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg
     4921 cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc
     4981 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg
     5041 gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag cgatcgcgcc
     5101 gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta tgccggactg
     5161 cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat
     5221 cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc
     5281 gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat
     5341 caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg tcaggttgac
     5401 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat cgacctctgg
     5461 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac
     5521 agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     5581 gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag tcctgccagc
     5641 agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg
     5701 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     5761 actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     5821 tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag atactttgcg
     5881 gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg cgctgatatt
     5941 cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta ctgcgcaggc
     6001 attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac cactggtctt
     6061 tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt
     6121 actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca tttactgcgc
     6181 attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     6241 agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggtgga
     6301 acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag cggggcgcag
     6361 cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat tccgccagct
     6421 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga cggctttgtg
     6481 gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta tgcgtcgact
     6541 gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc tgggtattca
     6601 tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc gactggcagc
     6661 acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag
     6721 cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta aggtatttga
     6781 ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa ccggtccggt
     6841 taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct
     6901 cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat
     6961 gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct
     7021 gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac
     7081 cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc
     7141 agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg cgctggcgaa
     7201 tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc
     7261 ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct
     7321 ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct
     7381 gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt taaccaacct
     7441 ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca
     7501 ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt
     7561 cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc cgctcacagg
     7621 cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt
     7681 ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc accgcgaccc
     7741 gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc
     7801 gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga gttgcgtgac
     7861 gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca
     7921 acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt
     7981 cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat
     8041 tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc
     8101 gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg ggtatcttgg
     8161 acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg gtgaacggat
     8221 gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt acctcgggcg
     8281 cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt
     8341 gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaaccaggc
     8401 ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc aatcgggcct
     8461 gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc cgcatatggt
     8521 gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca agctggatcg
     8581 caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc cgaaagcggg
     8641 cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc
     8701 cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac tggcagcgca
     8761 gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt
     8821 cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga
     8881 aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcgtc
     8941 cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat
     9001 tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt
     9061 ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg cggcgcggct
     9121 gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc cgccagagac
     9181 gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg cggagattaa
     9241 ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
     9301 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
     9361 gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc
     9421 cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
     9481 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct
     9541 aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAZOZX010000139         9537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZOZX010000139 DAZOZX010000000
VERSION     DAZOZX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.139"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUPT_003391"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUPT_003391"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0820826.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUPT_003392"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUPT_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0820827.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUPT_003393"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUPT_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACHUPT_003394"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACHUPT_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0820828.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACHUPT_003395"
     tRNA            4965..5038
                     /locus_tag="ACHUPT_003395"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACHUPT_003396"
     CDS             5173..6783
                     /locus_tag="ACHUPT_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0820829.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACHUPT_003397"
     CDS             6815..7234
                     /locus_tag="ACHUPT_003397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820830.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACHUPT_003398"
     CDS             8353..9099
                     /locus_tag="ACHUPT_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0820831.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACHUPT_003399"
     CDS             9397..>9537
                     /locus_tag="ACHUPT_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820832.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DAZOZX010000140         9429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZOZX010000140 DAZOZX010000000
VERSION     DAZOZX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.140"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            129..1826
                     /gene="maeA"
                     /locus_tag="ACHUPT_003400"
     CDS             129..1826
                     /gene="maeA"
                     /locus_tag="ACHUPT_003400"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0820833.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="ACHUPT_003401"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="ACHUPT_003401"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR0820834.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="ACHUPT_003402"
     CDS             3116..3400
                     /locus_tag="ACHUPT_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0820835.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="ACHUPT_003403"
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="ACHUPT_003403"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0820836.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="ACHUPT_003404"
     CDS             complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="ACHUPT_003404"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0820837.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="ACHUPT_003405"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="ACHUPT_003405"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0820838.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQSHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8450..9331
                     /gene="yddG"
                     /locus_tag="ACHUPT_003406"
     CDS             8450..9331
                     /gene="yddG"
                     /locus_tag="ACHUPT_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0820839.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2257 a         2488 c         2391 g         2293 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttgttacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gaggaagagt gcatcgtggt cgccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatggatgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cctataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaaggg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggctctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg taactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagcg actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaat gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcaccgc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tatttcgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     8461 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     8521 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     8581 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
     8641 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tatctggcgc tttccttagg
     8701 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     8761 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     8821 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
     8881 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     8941 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
     9001 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
     9061 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
     9121 taccttagga tttgcttata ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
     9181 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
     9241 cagcgccccg ctgtcgttct cgttctggca aggcgcacta atggtctgcg gcggttccct
     9301 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
     9361 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attcacctta
     9421 tatctcatt
//
LOCUS       DAZOZX010000141         9153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZOZX010000141 DAZOZX010000000
VERSION     DAZOZX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.141"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..923
                     /locus_tag="ACHUPT_003407"
     CDS             <1..923
                     /locus_tag="ACHUPT_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0820840.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUPT_003408"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUPT_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0820841.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUPT_003409"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUPT_003409"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0820842.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSNSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUPT_003410"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUPT_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0820843.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHUPT_003411"
     CDS             4446..4805
                     /locus_tag="ACHUPT_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0820844.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUPT_003412"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUPT_003412"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0820845.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUPT_003413"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUPT_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0820846.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHUPT_003414"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHUPT_003414"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0820847.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2091 a         2210 c         2473 g         2379 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatttgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atg
//
LOCUS       DAZOZX010000142         9029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZOZX010000142 DAZOZX010000000
VERSION     DAZOZX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.142"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUPT_003415"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUPT_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0820848.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUPT_003416"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUPT_003416"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0820849.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUPT_003417"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUPT_003417"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0820850.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUPT_003418"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUPT_003418"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0820851.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVQ
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUPT_003419"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUPT_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0820852.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHUPT_003420"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHUPT_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0820853.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHUPT_003421"
     CDS             6064..6606
                     /locus_tag="ACHUPT_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0820854.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUPT_003422"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUPT_003422"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0820855.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUPT_003423"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUPT_003423"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0820856.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHUPT_003424"
     CDS             8003..8260
                     /locus_tag="ACHUPT_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0820857.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHUPT_003425"
     CDS             complement(8236..8790)
                     /locus_tag="ACHUPT_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0820858.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2191 c         2251 g         2328 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gtgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgctgca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAZOZX010000143         8872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZOZX010000143 DAZOZX010000000
VERSION     DAZOZX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.143"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHUPT_003426"
     CDS             <1..439
                     /locus_tag="ACHUPT_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0820859.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUPT_003427"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUPT_003427"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0820860.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUPT_003428"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUPT_003428"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0820861.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUPT_003429"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUPT_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0820862.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACHUPT_003430"
     CDS             complement(4441..5148)
                     /locus_tag="ACHUPT_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFR0820863.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPGFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUPT_003431"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUPT_003431"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0820864.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACHUPT_003432"
     CDS             complement(6006..6209)
                     /locus_tag="ACHUPT_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0820865.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACHUPT_003433"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACHUPT_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..8769
                     /locus_tag="ACHUPT_003434"
                     /pseudo
     CDS             7873..8769
                     /locus_tag="ACHUPT_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            complement(8785..>8872)
                     /locus_tag="ACHUPT_003435"
     CDS             complement(8785..>8872)
                     /locus_tag="ACHUPT_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0820866.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2062 a         2416 c         2273 g         2121 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa acccaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
     8761 gaacaattcg gtaatgactc caacttactg atagtgtttt atgttcagat aatgcccgat
     8821 gaccttgtca tgcagctcca ccgattttga gaacgacagt gacttccgta cc
//
LOCUS       DAZOZX010000144         8838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZOZX010000144 DAZOZX010000000
VERSION     DAZOZX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.144"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..689
                     /locus_tag="ACHUPT_003436"
     CDS             <1..689
                     /locus_tag="ACHUPT_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFR0820867.1"
                     /translation="VKLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGE
                     DDKFDNFMRQHRFSKVNFDTQQMIHFINRFNMNKGLVDKNNNFFIQLIDQALRSTDDM
                     IKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVV
                     SSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALFPIFTASW
                     RATRIKNKGI"
     gene            912..1409
                     /gene="ubiC"
                     /locus_tag="ACHUPT_003437"
     CDS             912..1409
                     /gene="ubiC"
                     /locus_tag="ACHUPT_003437"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0820868.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1422..2294
                     /gene="ubiA"
                     /locus_tag="ACHUPT_003438"
     CDS             1422..2294
                     /gene="ubiA"
                     /locus_tag="ACHUPT_003438"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0820869.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2449..4872)
                     /gene="plsB"
                     /locus_tag="ACHUPT_003439"
     CDS             complement(2449..4872)
                     /gene="plsB"
                     /locus_tag="ACHUPT_003439"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0820870.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5043..5411
                     /gene="dgkA"
                     /locus_tag="ACHUPT_003440"
     CDS             5043..5411
                     /gene="dgkA"
                     /locus_tag="ACHUPT_003440"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0820871.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5521..6129
                     /gene="lexA"
                     /locus_tag="ACHUPT_003441"
     CDS             5521..6129
                     /gene="lexA"
                     /locus_tag="ACHUPT_003441"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0820872.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6202..7527
                     /gene="dinF"
                     /locus_tag="ACHUPT_003442"
     CDS             6202..7527
                     /gene="dinF"
                     /locus_tag="ACHUPT_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0820873.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7643..7852
                     /locus_tag="ACHUPT_003443"
     CDS             7643..7852
                     /locus_tag="ACHUPT_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0820874.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7894..8409)
                     /gene="zur"
                     /locus_tag="ACHUPT_003444"
     CDS             complement(7894..8409)
                     /gene="zur"
                     /locus_tag="ACHUPT_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0820875.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8727..>8838
                     /locus_tag="ACHUPT_003445"
     CDS             8727..>8838
                     /locus_tag="ACHUPT_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820876.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSI"
BASE COUNT         2101 a         2086 c         2345 g         2306 t
ORIGIN      
        1 cagtaaaact ggtgatgcaa ttaaaaattc tcaatattgt taataaaaac tttattgaga
       61 atatgaagaa aacatttagc cattgtcctt atataaaaga tccaattata cgtagttata
      121 tacattctgg ggaagatgac aagttcgata attttatgcg tcaacatcga ttcagtaagg
      181 tgaatttcga tacccaacag atgatccatt ttattaacag atttaatatg aataaagggc
      241 tggttgataa aaataacaat ttttttatcc aacttatcga tcaggcctta cgatcaacgg
      301 atgatatgat caaagcaaat gcctggtatc tttataaaga gtggattcgt agtgatgatg
      361 tttcacctat atttatagaa actgaagaaa agttaagaac ctttaacact aataaattaa
      421 cacgaaacga taatatattt atcctgttct cctctgtcga tgatgggcct gttatggtgg
      481 taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca
      541 cgtgtatcta taaatccaga cataagatgt tgcctattaa tcttactcag gaaacacttt
      601 tcagctccaa atctcatggt aaatatgcgc ttttcccaat ttttactgcg agttggcgag
      661 ctactcgtat aaagaataaa ggtatttaag taaaggaaaa catcaccgtt cctagcatca
      721 tgggcggtga tgcccctatg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt
      781 caacagcgtt ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa
      841 tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg
      901 gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag
      961 agatccctgc cctggatccg caactgctcg actggctgtt gctggaggat tccatgacaa
     1021 aacgttttga acagcaggga aaaacggtga gtgtgacgat gatccgcgag gggtttgtcg
     1081 agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac
     1141 gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg
     1201 tatcaacatt aagcgggccg gaactagcgt tacaaaaatt gggtaaaacg ccgttaggac
     1261 gctatctgtt cacatcatcg acactaaccc gggattttat cgagataggc cgtgatgccg
     1321 gactgtgggg gcgacgttcc cgcctgcgat taagcggtaa accgctgttg ctaacagaac
     1381 tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc
     1441 agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac
     1501 tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt cctcagctct
     1561 ggatcatggc ggtgtttgtc gcgggtgtct ggctgatgcg cgctgctggt tgcgtggtga
     1621 atgattatgc tgatcgcaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc
     1681 ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt
     1741 cgtttttact ggttctgacg ctgaatacga tgaccattct gttgtcgatt gccgctctgg
     1801 cattggcgtg ggtgtacccg tttatgaagc ggtataccca tctcccgcaa gtggtgctgg
     1861 gcgcggcgtt tggctggtcg attccaatgg cttttgccgc tgtgagtgag tcggtgccat
     1921 tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt
     1981 atgcgatggt tgaccgcgat gatgatgtga agattggcat taaatccacg gcaatcctgt
     2041 tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg gcactgatgg
     2101 cgatcatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt gtggtggctg
     2161 gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta
     2221 aagcatttat gaataataac tatgttggtc tggtactgtt tttaggactg gcaatgagtt
     2281 actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc
     2341 tgatgcgttg cgcttctcag gcctacccgg tgaatcgcaa tttattgaat ttgcaggtca
     2401 tggtaggccg gataaggcgt tttcgccgca tccggcaatt ctctctgatt acccttcgcc
     2461 ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg
     2521 ataaaccttc atcgtttctg ctggttcggc atcgccgcta tcgctgatat acccttcatc
     2581 acgcagcgtc agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat
     2641 gccgtgcagc acggagagac gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg
     2701 gttgatcgac gggttggcac tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc
     2761 gcgcgcgcct gcggccagca gctgtagggt gcgagaatgc gccgggttga tatgcaactc
     2821 atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac
     2881 gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag
     2941 cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc
     3001 cgccatcagc gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag
     3061 caccgcttgc tcgcgtggca gaatgatgat gtcgccgata gtgtctttct cgacttcaaa
     3121 cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga
     3181 gtccgtggag taaggtacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa
     3241 ctgctcgcgg gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat
     3301 ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac
     3361 cgtcggcgtt aaccaggccg gacgcaccgc ttcgatggga tcgatagatt cacgccagtc
     3421 tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc
     3481 ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc
     3541 tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc
     3601 gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt
     3661 catcgacagc gtaccggttt tcggatccag caaacgcccc gtgcgggaac gaccgccttc
     3721 cacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac
     3781 ggtggaataa agtttattgc ctttaaaagt acggcgaata aagaacgccc ccagacggcg
     3841 gaaaatcggc ccggccggcc agaagttcag gttgatcccg gcggcgatat gcggcggcac
     3901 cagtccctgg tggtacagca cgtaagaaag tagcaggtag tccatgtgac tgcggtggca
     3961 aggcacatac accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg
     4021 gacgttgatg ccctgataaa atcggttcca ggtgaagccc agaatgcggt cagtcaggcg
     4081 gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg
     4141 cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc
     4201 gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg ggcctacggc
     4261 agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gagcgatagt
     4321 tttatccgtg ccgtgttcat ccgccatacg gcgcagcgac actgacggcg agaaacgcac
     4381 aaaactgtcg cgaccgagcc acagtacagc gaaaaatttc tgtacgccgt taagcatacg
     4441 cagcggcggg ttcacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac
     4501 tggcaccatc tgcacatcca gatttgggtt gctacggtgc aaatcgagat agtcgtggaa
     4561 aagcttaata gactcttctt tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat
     4621 gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa
     4681 atcatgtgcc agacactggg cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa
     4741 aacgtacata attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat
     4801 agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg
     4861 ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata
     4921 gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac
     4981 ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt
     5041 caatggccaa taatacaact ggattcaccc gaattatcaa agctgctggc tattcctgga
     5101 aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat
     5161 tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc aattacccgc gtgctgctta
     5221 tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg
     5281 ttgaccgaat tggctctgaa taccatgagc tttccggacg cgcaaaagat atgggatccg
     5341 ccgcggtgct gattgccatt atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc
     5401 attttggata acccttccag atttcgataa atctctggtt tattgtgcag tttatggttc
     5461 caaaatcgcc ttttgctgta tatactcaca gtataactgt atatacaccc agggggcgga
     5521 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     5581 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     5641 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
     5701 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
     5761 cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc
     5821 gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     5881 aaagatatcg gcattatgga tggcgacttg ctggcagtgc ataaaactca ggatgtacgt
     5941 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa
     6001 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac
     6061 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac
     6121 tggctgtaac atatctctga gaccgcgatg ccgcttggcg tcgcggtttg tttttcatct
     6181 ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc actctggcat
     6241 ctcgccttac ccatgatttt ctccaatatc accgttccgt tgctgggact ggtcgatacg
     6301 gcggtaattg gtcatcttga tagtccggtt tatttgggcg gcgtggcggt tggtgcaacg
     6361 gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact
     6421 gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaaccgttg
     6481 ctgttggcgt tgggggccgg ggcgttaatt gcgctgttgc gtacgccgat tatcgatctg
     6541 gcgctgcata ttgttggcgg tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa
     6601 atccgctggt taagcgctcc ggcgtcgctg gcgaatctgg tattactcgg ttggttactc
     6661 ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt
     6721 gtgctggatg tctggctggt gatggggctg catatgaacg tgcagggcgc ggcgctggcg
     6781 acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc
     6841 aaactacgcg gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc
     6901 ttgctggcgc ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc
     6961 gcgatcaccg tacttggtgc gcgactgggg agtgacatta tcgctgttaa cgcggttctg
     7021 atgacgctac tcacctttac cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg
     7081 cactccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt atggcgggca
     7141 gcgtgccgcc agtcggggat tgtagcgtta ctattttcgg tggtttattt gctggcaggg
     7201 gaacacatca ttgcgttgct gacgtcgtta acccagattc agcagctggc tgaccgctat
     7261 cttatctggc aggtgatttt gccgttggtt ggcgtctggt gttatctgct ggacggcatg
     7321 tttataggcg caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt
     7381 tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gctggcatta
     7441 accgtctttc tggcgttgcg cgggctttct ctggcggcta tctggcggcg tcactggcgc
     7501 aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc
     7561 aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca
     7621 acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt
     7681 taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga
     7741 aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca
     7801 ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc
     7861 tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac
     7921 agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc
     7981 acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag
     8041 cgtatgcata atgtcttcca cgccttctgc acactcttct ttcaccgcgc cgcagcgatc
     8101 gcaaataaac atggctgacg tatgcgtggg ctggtcgaac agatgacaga gcacataact
     8161 gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata
     8221 aaccgttggc ggcttggctt gcggttcagc ttcgcgcagt aaatcaagca gatcataagc
     8281 gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag
     8341 gcgcacatta cgctgcgcgc agattttttc agcctgcgct aataactcct gcgttgtggt
     8401 cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag
     8461 gaaaaacacc gagaaccctt attgttgccg taatgttgat tttctgtttt gtaggtaagg
     8521 tgttgtgttg ccttatcgta ccattatcaa cacgataata attaataatt catttttaaa
     8581 taaattgctt caagttaact cgctatttat ttaactgggt ttaaacgcat acctgtagtt
     8641 cagaaattat gagcgatttt tgatagtgac cagatggcaa aagaattata aggaggacac
     8701 ggtacgacaa aaaaggtaga tgaatcatgt tgaagataat acccggtgca actggatact
     8761 ttaataaaac actcaattca aatcaattcg aaaataaaga tgcaaccaaa gataaattgg
     8821 ataatagagg ttcaatca
//
LOCUS       DAZOZX010000145         8778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZOZX010000145 DAZOZX010000000
VERSION     DAZOZX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.145"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            82..390
                     /locus_tag="ACHUPT_003446"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHUPT_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..2289
                     /locus_tag="ACHUPT_003447"
                     /pseudo
     CDS             362..2289
                     /locus_tag="ACHUPT_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2273..2476
                     /locus_tag="ACHUPT_003448"
     CDS             2273..2476
                     /locus_tag="ACHUPT_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR0820877.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2473..4066
                     /locus_tag="ACHUPT_003449"
                     /pseudo
     CDS             2473..4066
                     /locus_tag="ACHUPT_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            4056..5561
                     /locus_tag="ACHUPT_003450"
     CDS             4056..5561
                     /locus_tag="ACHUPT_003450"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0820878.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5598..5945
                     /locus_tag="ACHUPT_003451"
     CDS             5598..5945
                     /locus_tag="ACHUPT_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0820879.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            6003..7031
                     /locus_tag="ACHUPT_003452"
     CDS             6003..7031
                     /locus_tag="ACHUPT_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0820880.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHUPT_003453"
     CDS             7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHUPT_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0820881.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7481..7858
                     /locus_tag="ACHUPT_003454"
     CDS             7481..7858
                     /locus_tag="ACHUPT_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0820882.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7845..8429
                     /locus_tag="ACHUPT_003455"
     CDS             7845..8429
                     /locus_tag="ACHUPT_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0820883.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8426..>8778
                     /locus_tag="ACHUPT_003456"
     CDS             8426..>8778
                     /locus_tag="ACHUPT_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0820884.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
BASE COUNT         2050 a         2070 c         2690 g         1968 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaagt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgctggc aacttgatct ggctgtgcag ttgcatcccg caaggatgca
     2221 actgcacaac cgacccttga agaactggca gcgaagctgt ccggaggagt gaatggttac
     2281 agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg gtaaacgggt
     2341 tgtgtcggta cagaaagacg gtcgcaggat cgaatatacg gcagcgtccc tgaatgagct
     2401 taaccgtgcg atcaacgatg cggagtcggt tctgggtacg acccgacgcc gccgaccgct
     2461 gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt tccgcttcgt
     2521 gagagtctca gctacaacgg aggcggcgca ggatttggcg ggcaaatggc agagtggtta
     2581 ccaccggcgc agagtgccga tgcggccctg ttgcctgcat tgcgtctggg gaatgcccgg
     2641 gcagatgatc tggtgcgcaa taacggtatc gcggctaatg cggtggccct gcataaggat
     2701 catatcgtcg ggcacatgtt tcttatcagc taccgtctga actggcgttg gctggggatg
     2761 cgggagacca cggcaaaaag ttttgtcggg gaggtggaag cagcctggtc ggaatacgca
     2821 gaaggaatgt ttggcgagat cgacgtggaa gggaaacgca cgtttacgga atttatccgt
     2881 gaaggggtgg gcgtgcatgc ctttaacggc gaaatctttg tgcagccggt ctgggatacg
     2941 gaaaccacgc agttattccg tacgcgtttt aaagccgtga gtccgaaacg ggtggacacg
     3001 ccaggacacg gtatggggaa ccactttctg cgggccgggg ttgaggtcga tcggtatggg
     3061 cgggcgattg cgtaccatat ctgtgaggat gattttccgt tctctggtgc cggacgctgg
     3121 gagcggatcc cgcgtgagct tcccaccggg cgtccggcca tgctgcatat ttttgagccg
     3181 gtggaggacg ggcagacccg tggggctaat cagttttaca gcgtaatgga gcggatgaag
     3241 atgctggact ccctgcaggc cacgcagctt cagtcggcca ttgtgaaggc gatgtatgca
     3301 gccacaatcg aaagtgaact tgataccgaa aaggcatttg aatatatcgc gggggcaccg
     3361 caaggtcagc aggataatcc gcttattaat attctggaga agttcacccg ctggtatgac
     3421 acgaataacg tgacgctggg cggtgttaaa attccgcacc ttttttcctg gcgatgcgct
     3481 gaacctgcag acagcgcagg attcagacaa tggattttcg gcgcttgaac aggcgctgct
     3541 gcggtatatt gcagccggtc ttggcgtttc ctacgaacag ttgtcccgtg attactcgaa
     3601 ggtcagttac tcaagtgccc gcgcctccgc caatgagtcg tggcgctatt ttatggggcg
     3661 gcgaaaattt attgcggccc ggctggccac gcagatgttt tcctgctggc tggaagaggc
     3721 gcttcttcgg gggattattc gtccgccacg ggcgcgtttt gatttttacc aggcgcgttc
     3781 agcctggtca cgggcagagt ggattagctc cggaagaatg gccattgacg ggctcaagga
     3841 ggtccaggaa tcggtgatgc gcattgaggc cggactgagc acgtatgaga aagaactggc
     3901 actgatgggc gaagattatc aggacatttt ccgccagcag gtcagggaat ccgcagagcg
     3961 cgaaaaagcc ggactctcac gtccggtgtg gatagcccag gcgtatcagc agcagatagc
     4021 ggagagtcgc aggccggaag aggagacaac atcacgtgag acgtaatctt tcacatatta
     4081 tcgctgcggc attcaatgag ccgctgcttc tggagcccgc ctatgcgcgg gttttctttt
     4141 gcgcgctcgg gcgtgagatg ggcgcagcaa gcctttcggt accgcaacag caggtacagc
     4201 ttgacgctcc cgggatgctg gctgaaacgg acgagtacat ggccggaggc aaacgacctg
     4261 cccgtgttta ccgggtggtg aacggtattg ctgtactgcc ggtgaccggc acgctggtac
     4321 accggctggg tggcatgcgg ccattttccg gaatgactgg ctatgacggc attgtcgcct
     4381 gtcttcagca ggcaatggcg gacactcagg tacggggcgt actgctggac attgacagtt
     4441 ctggcgggca ggccaccggc gcgtttgact gcgctgacat gatttaccgt ctccggcagc
     4501 tgaagcctgt ctgggcactg tgcaatgaca cggcctgttc agccgccatg ttactggcat
     4561 cggcctgctc ccgacggctg gttacccaga catcccgtat cggctccatt ggggtgatga
     4621 tgggccatgt cagctatgcc ggtcatctgg cacaggccgg tgtggatatc acgctgattt
     4681 acgccggatc gcacaaggtg gatggcaatc agtttgaatc cctgccagca aaggttcgcc
     4741 aggacatgca gcagcgcatt gatgcggcgc gccggatgtt tgctgaaaaa gtggcgatgt
     4801 ataccggttt atctgtggat gcggttatgg gaacagaggc cgccgttttt gaaggtcagt
     4861 ccggtattga ggccgaactg gcggatgaat taatcaatgc gtcggacgcc atcagcgtga
     4921 tggctgcgac gctgaacaca cacgatacag gaggcactat gccgcaatta actgcaacgg
     4981 atgctgccgc gcaggagaac cagcgagtga tggggatcct gacgtgtcag gaagcgaaag
     5041 gacgtgaaca actggccacg atgctggcag ggcaacaggg tatgagcgtt gagcaggccc
     5101 gggcgattct ggccgtggcg gcaccgcaac aatctggtgt atctgcgcag agtgaagccg
     5161 atcgcattat ggcatgtgaa gaggcgaaag gacgtgaaca actggcggca acgctggcgg
     5221 cgatgccgga gatgacggtg gaaaaagccc gtccgattct ggctgcttca ccgcaggcga
     5281 atgccggacc ctcacttcgt gatcagatta tggccctgga tgaggcaaaa ggggcagagg
     5341 cgcaggctga aaaactggcg gcctgtccgg ggatgaccgt ggagaatgcc cgggctgtac
     5401 tggcggcagc ctcaggcagg gcagaaccgg tttctgcatc cacaaccgcc atgtttgaac
     5461 atttcatggc gaaccattca ccggcagcgg tacagggtgg cgtgccacag acgtcagcag
     5521 acggtgatgc ggacgtgaaa atgctcatgg ccatgccatg aagtcagtgc tgaccatcaa
     5581 tatgaggttt ttacaatatg gtaacgaaaa ccatcactga acagcgtgcg gaagtacgta
     5641 tttttgccgg taatgatccg gcccataccg ccacaggctg cagcgggatt tcctcggcaa
     5701 caccggctct gacgcccctg atgctggatg aggataccgg gaaactggtg gtctgggacg
     5761 gacagaaggc aggccatgtg gttggcatac tggtactgcc gcttgaaggt acagagacgg
     5821 tgctgacgta ttacaagtcg gggacctttg cgacggaggc catccactgg cctgagagag
     5881 tggatacaca caaaaaggcg aatgcctttg ccggcagtgc cctgagtcac gcagcgctgc
     5941 cgtaacattt tatcaggtca ctgcggtagc ctgactgatt tctgaatgaa aggaactgat
     6001 ttatgggatt gtttacgacc cgccagttac tcggttatac cgaacaaaaa gttaaatttc
     6061 gtgcgctgtt tctggagctg tttttccgcc gtacggtgaa tttccatacc gaagaggtaa
     6121 tgctggacaa aattaccgga aaaacgccgg tggcggccta tgtctccccg gttgttgaag
     6181 gaaaggtgtt gcgtcaccgt ggcggtgaaa ctcgtgtact gcgcccgggc tacgttaagc
     6241 cgaaacacga atttaattac cagcaggcgg tggagcgtct tcccggtgaa gatccggccc
     6301 agctgaacgg cccggcttac cgtcgcctgc gtatcatcac cgataacctc aaacaggaag
     6361 agcacgccat tgtccaggtg gaagagatgc aggcggtgaa tgccgtgctg tatggcaaat
     6421 acaccatgga aggagagcag tttgaaactg ttgaggtcga ttttggccgg tcggtgggaa
     6481 ataacattac gcaggcagga gggacggagt ggagcgctca ggaccgcgat acctttgatc
     6541 cgacgcatga tctggatgcg tactgtgatt ttgcttccgg caccatcaat attgccatta
     6601 tggatggtaa ggtctggcgt ctgctgaatg gctttaagct gttccgcgaa aaactggata
     6661 cccgtcgtgg ctcaaattca cagctggaaa cggcagtgaa agacctgggg gcggtggtgt
     6721 ccttcaaagg gtattatggt gatctggcaa tcgtggtggc aaaaacgtcc tatgtggcag
     6781 aggacggtac cgaaaaacgt tatctgccgg acggcacact ggtactggga aatacggccg
     6841 cagagggtat tcgttgctac ggtgccattc aggatgcgca ggcattgtct gaaggcgtgg
     6901 tggcgtcttc ccgttatccg aaacactggc tgaccgtggg tgatccggcg cgtgaattca
     6961 ccatgacgca gtccgcgccg ctgatggtgc tgccggatcc ggatgagttt gtggtggtgc
     7021 aggtgaaata atccgtgagc gggagctaaa tgctcccgtt tttcattcaa tcaggagcgg
     7081 gtatggctac gaaagaagaa aacctgaaac gtctttgtga gctggctgag aggctggggc
     7141 gtgaaccgga tgtctccggt agtgcggcag atattgcgca gcgagtggcg gaactggagg
     7201 aagagcttgg agatgcgggg gaggtcgcag agccggatac gtactcacca tcgaaagatg
     7261 atccgaccgc gcataaaggc gagtcagttc ctgaacagcc aggcattgtt accggagacg
     7321 aaacggggca ggttacggtg gtggcactgg cgacactgca tacaaaatgt aatggttcag
     7381 tgatatttgt ttcaccggga acgtcgtttc gtgtttctgc tgaggtggct gccagcatgg
     7441 cggcaggtgg tctggcaaaa aggcagtaac aggagatgtc gtggacggtt tcgataatct
     7501 gtttgatgct gcgcttgccg gtgtcaatga agtcattctc cgtgatatgg ggatcagcgc
     7561 cgtcattacg tccggagaac ttgaggggac acacctgaca ggggtttttg atgatcccga
     7621 aagtatttct tttgttgccg gggggatccg gcttgaggat tcctcaccgt gcctgtttgt
     7681 gaaaacggca gatatatcgc agttacgccg tcaggatacg ttaactatcg gggatgattc
     7741 tttctttgtg gatcgtatta ctccggatga cgggggatgc tgttatatcc ggttaaggcg
     7801 gggcagtcct gccccacaga ataatgccag aatgaggcga tatgatgaag ggacttgaga
     7861 atgccatccg caatctgaac agcctcgata cccgcatggt gccacagacc agcgcatggg
     7921 cggtaaaccg tgtggcggca aagattgttt ctgtcgccac acggcaggtt gcgcagaata
     7981 ccgttgccgg ggataaccag gtgaaaggta ttcccctgaa aacggtcagg gagcgtgtca
     8041 ggctgcttaa agccagcccc tcaggaaaaa tgtatgcccg catgcgtgtt aaccggggta
     8101 acctgcccgc cattaagctg ggtaccgcac aggtcagact ggcccgctcc cggcatggca
     8161 gcaactcacg tcatcgtggc agcgtactga aagtggggaa ataccttttc cgggatgcgt
     8221 ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgac ggcaaaaatc
     8281 gttaccccat tgatgtggtg aaaatcccga tgtccggacc gctgacacag gcatttgaag
     8341 atgcccgcga ccgcatcatt gctgcggaaa tgccgaaaca gctggggtat gccctgaaac
     8401 aacaactgag gttacatctg agtaaatgaa ccgacacaca caaatccgtc agagcgtgct
     8461 ggcacgcctt cgggaacagt gtggagacag cgccacgttt tttgacgggc ttccggcatt
     8521 tattgatgcg caggaactgc ccgccgtggc ggtgtggctg agtgatgcgc agtacaccgg
     8581 aaaaatgacg gatgaggacg actggcaggc tgttctgcat attgccgttt tcgtccgtgc
     8641 acaggcgccg gattctgagc tggatacgtg gatggagaac accatatttc cggccatgaa
     8701 ggatattccg gtgctttccg gactcatcga caccatgatc ccactcggtt ttaactatca
     8761 acgtgataat gagatggc
//
LOCUS       DAZOZX010000146         8720 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZOZX010000146 DAZOZX010000000
VERSION     DAZOZX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.146"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(435..641)
                     /locus_tag="ACHUPT_003457"
     CDS             complement(435..641)
                     /locus_tag="ACHUPT_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0820885.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACHUPT_003458"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACHUPT_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0820886.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHUPT_003459"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHUPT_003459"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0820887.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHUPT_003460"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHUPT_003460"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHUPT_003461"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHUPT_003461"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0820888.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHUPT_003462"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHUPT_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8720
                     /gene="kdpE"
                     /locus_tag="ACHUPT_003463"
                     /pseudo
     CDS             8037..8720
                     /gene="kdpE"
                     /locus_tag="ACHUPT_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1869 a         2243 c         2501 g         2107 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt
//
LOCUS       DAZOZX010000147         8707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZOZX010000147 DAZOZX010000000
VERSION     DAZOZX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.147"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHUPT_003464"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHUPT_003464"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0820889.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHUPT_003465"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHUPT_003465"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0820890.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHUPT_003466"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHUPT_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR0820891.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHUPT_003467"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHUPT_003467"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0820892.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHUPT_003468"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHUPT_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0820893.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHUPT_003469"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHUPT_003469"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0820894.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHUPT_003470"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHUPT_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR0820895.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..>8707)
                     /locus_tag="ACHUPT_003471"
     CDS             complement(8064..>8707)
                     /locus_tag="ACHUPT_003471"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0820896.1"
                     /translation="NGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLL
                     MQSFEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEK
                     MYCYYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKE
                     PMTADSISSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         2378 a         2060 c         1952 g         2317 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttg
//
LOCUS       DAZOZX010000148         8689 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZOZX010000148 DAZOZX010000000
VERSION     DAZOZX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.148"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUPT_003472"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUPT_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0820897.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACHUPT_003473"
     CDS             696..914
                     /locus_tag="ACHUPT_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0820898.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACHUPT_003474"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACHUPT_003474"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0820899.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUPT_003475"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUPT_003475"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0820900.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUPT_003476"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUPT_003476"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0820901.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUPT_003477"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUPT_003477"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0820902.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUPT_003478"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUPT_003478"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0820903.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUPT_003479"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUPT_003479"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0820904.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..8672
                     /gene="dapB"
                     /locus_tag="ACHUPT_003480"
     CDS             7851..8672
                     /gene="dapB"
                     /locus_tag="ACHUPT_003480"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0820905.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         1990 a         2160 c         2462 g         2077 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aataatttgt aaccacaaaa tgtttatta
//
LOCUS       DAZOZX010000149         8687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZOZX010000149 DAZOZX010000000
VERSION     DAZOZX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.149"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..80
                     /gene="yjbL"
                     /locus_tag="ACHUPT_003481"
     CDS             <1..80
                     /gene="yjbL"
                     /locus_tag="ACHUPT_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0820906.1"
                     /translation="IIAKIDLFIQRITHNLWHARKKMCF"
     gene            104..811
                     /locus_tag="ACHUPT_003482"
     CDS             104..811
                     /locus_tag="ACHUPT_003482"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR0820907.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            1174..2211
                     /gene="dusA"
                     /locus_tag="ACHUPT_003483"
     CDS             1174..2211
                     /gene="dusA"
                     /locus_tag="ACHUPT_003483"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0820908.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2345..2587
                     /gene="pspG"
                     /locus_tag="ACHUPT_003484"
     CDS             2345..2587
                     /gene="pspG"
                     /locus_tag="ACHUPT_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0820909.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(2753..3736)
                     /locus_tag="ACHUPT_003485"
     CDS             complement(2753..3736)
                     /locus_tag="ACHUPT_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0820910.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3819..5234
                     /gene="dnaB"
                     /locus_tag="ACHUPT_003486"
     CDS             3819..5234
                     /gene="dnaB"
                     /locus_tag="ACHUPT_003486"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0820911.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5287..6366
                     /gene="alr"
                     /locus_tag="ACHUPT_003487"
     CDS             5287..6366
                     /gene="alr"
                     /locus_tag="ACHUPT_003487"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0820912.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6619..7812
                     /gene="tyrB"
                     /locus_tag="ACHUPT_003488"
     CDS             6619..7812
                     /gene="tyrB"
                     /locus_tag="ACHUPT_003488"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0820913.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(8315..8519)
                     /locus_tag="ACHUPT_003489"
                     /pseudo
     CDS             complement(8315..8519)
                     /locus_tag="ACHUPT_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2187 a         1992 c         2168 g         2340 t
ORIGIN      
        1 taattattgc aaaaatagat ttgtttattc aacgaatcac gcataattta tggcatgcac
       61 gtaaaaaaat gtgtttttaa ttgagcacat aaatgatctc aaaatgtggg tcaataagta
      121 tactgacgat tgcactgatg aggatttaaa cgatcgtgac tttattgcat cagttgttga
      181 ccgggctatt tttcatttcg cgattaatag tatatgtaat cctggggata ataaagatgc
      241 aatgcccatt gaacaatgta cttttgatgt agaaactaag aatggccttc cctccacggt
      301 tcagctattt tatgaggaat ctaaggataa tgaaccttta gcgaatatac attttcaagc
      361 aataggttct ggttttttaa cgtttgttaa tgcctgccag gaacatgatg acaacagctt
      421 aaaattattt gcttcgctgt taatttcact ttcatattct agtgcctacg cagatttatc
      481 agaaacagtg tatattaatg aaaataatga gagctacctg aaagctcagt ttgaaaaatt
      541 atatcaacgt gatatgaaga agtacctggg agagatgaag cgtctggcag atgggggaga
      601 aatgaatttt gatggctatc tggataagat gtcacatctg gtgaatgaag gaacgctcga
      661 tcctgatatt ttaagcaaaa tgcgagatgc tgcaccacaa ttaattagct tcgcgaagtc
      721 gtttgaccca acctcaaagg aagagattaa aatacttaca gacacttcta aattaattta
      781 tgacttgttc ggggttaaat cggagaaata atatgtgaag ttcttcgata gtatggaagg
      841 cattatataa aaggacccaa tatttattgg gttctttttt ctctatcaat gctattagca
      901 gggagatata tcaccagagt ttaatgtgtg attttttatt tatcgtcgaa cctggattgt
      961 ttatcattgg ccttaacaaa gttaacggct aataagatta tttccatcac ttcgtgagag
     1021 cttcatgcct tgagaggatc tcaattttct tttgcaatga gacaggcgct tcctgttgtt
     1081 atggtatagt accccgctat tgagcctcct gaacagtgaa gctgaataac ataaccccat
     1141 gatatatcga taaaataatc tctacatttg aaaatgcacg gtaattctga aatgcaaaaa
     1201 atcaaccaaa ccagcgcaat gcctgaaaaa actgacgttc actggagtgg tcggtttagt
     1261 gttgcaccaa tgctcgactg gacggacaga cattgccgct atttcttgcg tctgctttcc
     1321 cgcaatacgt tgctttatac cgaaatggtg accacagggg cgataattca cggtaaaggt
     1381 gattatctgg cgtacagtga agaagaacat ccggtagcgt tgcaactggg cggtagcgat
     1441 ccggcggcgc tggcgcagtg tgcaaagctg gcagaagcgc gcggatatga tgagatcaac
     1501 ctgaatgtcg gctgcccgtc tgaccgggtg cagaacggca tgtttggtgc gtgtctgatg
     1561 ggtaatgcgc agctggttgc cgactgcgtg aaagcgatgc gcgatgtggt gtcgattccg
     1621 gtgacggtga aaacgcgtat tggcatcgac gaccaggaca gctatgaatt tctctgcgat
     1681 ttcatcaata ccgtttccgg caaaggcgag tgtgagatgt tcatcatcca cgcacgtaaa
     1741 gcctggcttt cggggttaag cccgaaagaa aaccgtgaaa tcccgccgct cgattatccg
     1801 cgtgtgtatc aactgaagcg tgacttcccg catctgacaa tgtcgattaa cggtggtatc
     1861 aagtcgctgg aagaagctaa agcgcatttg caacatatgg atggcgtgat ggtcgggcgc
     1921 gaggcgtatc agaatccggg tattctggcg gcggtagacc gggagatctt tggttcctcg
     1981 gataccgatg ccgatccggt ggcagtagtg cgtgccatgt atccgtacat tgagcatgaa
     2041 ctcagccagg ggacgtatct cggccatatt atccggcata tgctgggttt gttccagggt
     2101 attcctggcg cgcggcagtg gcggcgttat ttaagtgaaa atgcccataa agcgggtgct
     2161 gacattaacg tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc
     2221 aaaaagtagt caaattcacc acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc
     2281 aatagattat ttttggcatg attcttgtaa tgccagcaag agatttcata tttgggagag
     2341 catcatgctg gaactacttt ttgtgattgg cttttttgtc atgctgatgg tcaccggcgt
     2401 ttcgttgctg ggcattatcg ccgcgctggt tgtggcgacg gccattatgt tcctcggcgg
     2461 tatgctggca ttgatgatta agttgctgcc gtggttacta ctggcgattg cggtggtgtg
     2521 ggttattaag gcgattaaag caccaaaaat gccgaaatat cagcgttatg accgctggcg
     2581 ttactaaggg attgtgcgga tgatcacaac ctaaggtttt atccttagaa caaaatagga
     2641 attgataatc aaatctgtca ctattgcgcc tctaacagat tcatcgtgct gtaccctaca
     2701 tacagccgaa ctataaaaag aaagggcttc ccaggtggaa gccctatttc ttttatggaa
     2761 tcagcaggct ggaaccttgc gtcgcccggc tttccagaat ctcatgcgca cgctgcgcat
     2821 ccttcagcgg atatttctgc tgctcggcga catcgacctt aatcacaccg ctggcaatca
     2881 aagagaacag ttcattactg gcctcggtta attcctcccg cgtggtgata tagccttgca
     2941 gggaagggcg tgtcacatac aacgagcctt tttgattgag aatgcctaag ttcacaccgg
     3001 taaccgcacc tgatgagttg ccaaaactga ccattaagcc gcggcgttgc aggcaatcca
     3061 gcgaccgttc ccaggtgtct ctgcccacgg aatcgtacac tacgcgcact ttcttaccgc
     3121 cggtgatctc tttcaatcgc tcgaccagat tctcttcacg atagttaata acctgccacg
     3181 cgccggcttt tagcgcgctc tgcgcttttt gcgcggttcc tacggtgccg atgagtttcg
     3241 cgcccagggc ttttgcccac tggcaggcaa ttaagccaat gccgccagcc gctgcgtgga
     3301 acaggaactg ttcatcgggt ttaatttcat aggttttgcg cagcagataa taaaccgtta
     3361 agcctttcag gaaggatgcc gcagcttgct caaaagaaat tgccgcaggc agaatcgccg
     3421 ctttatccgc attaatgtta tgcacagagc tgtaagcgcc taacgccgac tgcgcataga
     3481 ctacatgatc gccagcctta atatgcttta caccactgcc gactttactc acgatgcctg
     3541 ccgcttcggt acctaatccg ctgggtagcg atggcggcgg gtaaaggccg ctgcggatat
     3601 acgtgtcgat aaaattgatg ccgatggctt tattttcgac ctggatttca ttctccgccg
     3661 gatcggcagg agtgaactct acggcttgaa gtacttccgg gccaccgtgc ttgtgaaatt
     3721 caattcgtgt tgccatgtgt cctccagaac gtatcgtcag ggtctgcttc atatggtaaa
     3781 gtttcgaccc attctttatt tcggtaactc cattcactat ggcaggaaat aaacccttca
     3841 acaaacagca ggctgaaccc cgcgaacgcg atccacaagt tgccgggctg aaagtgcctc
     3901 cgcactcgat cgaagcggag cagtcggtgt tgggcggttt aatgctggat aacgaacgct
     3961 gggatgatgt agccgagcgt gtggtagcag acgattttta cacccgccca caccgtcata
     4021 tctttactga aatggcgcgt ttgcaggaaa gcggtagtcc tatcgatctg attacccttg
     4081 cggaatcgtt ggaacgccag gggcaactcg atagcgtcgg tggttttgct tatctggcag
     4141 agctgtcaaa aaatacgcca agtgcggcga acatcagtgc ttatgcggac atcgtgcgtg
     4201 aacgtgccgt tgtccgtgag atgatctcgg ttgcgaatga gattgctgaa gccggttttg
     4261 atccgcaggg gcgtaccagc gaagatctgc tggaccttgc tgaatcccgc gtctttaaaa
     4321 ttgccgaaag tcgtgcaaac aaagacgaag ggccgaagaa catcgccgat gtgctcgacg
     4381 caaccgtggc gcgtattgag cagttgtttc agcagccaca cgatggcgtt accggggtaa
     4441 acaccggtta tgacgatctc aacaaaaaaa ccgctggctt gcagccgtcg gatttgatca
     4501 tcgtcgccgc gcgtccgtcg atgggtaaaa caacatttgc gatgaacctc gtcgaaaacg
     4561 cggcaatgtt gcaggataaa ccagtactta tcttctcgct ggagatgcca tcagaacaga
     4621 tcatgatgcg ttctctggcg tcgctgtcgc gcgttgacca gactaaaatc cgtaccgggc
     4681 agctcgatga tgaagactgg gcgcgcattt ccggcaccat gggtattttg ctcgaaaaac
     4741 gcaatatcta tatcgatgac tcttccggcc tgacgccaac ggaagtgcgt tcccgcgcac
     4801 gccgtattgc ccgtgaacac ggcggcatcg ggcttatcat gatcgactac ctgcaactga
     4861 tgcgcgtacc ggcgctttcc gataaccgta cgctggaaat tgcagaaatc tcccgctcgc
     4921 tgaaagcact ggcgaaagaa ctgaacgtgc cggtggtggc gctgtcccag ttgaaccgtt
     4981 ctctggaaca acgtgccgac aaacgtccgg tcaactccga cctgcgtgaa tctggctcta
     5041 tcgagcagga tgcggacttg atcatgttta tctatcgtga tgaggtgtat cacgaaaaca
     5101 gtgatttaaa aggcatcgcg gaaattatta tcggtaaaca acgtaacggc ccaatcggga
     5161 cggtacgcct gacctttaac ggtcaatggt cgcgcttcga caactatgcg gggccgcagt
     5221 acgacgacga ataataatta ttttatgaat taggtaatta aagcaaacaa ttatcaagga
     5281 acacaaatgc aagcggcaac tgttgtgatt aaccgccgcg ctctgcgaca caacctgcaa
     5341 cgtcttcgtg aactggcacc tgccagtaaa atggttgcgg tggtgaaagc gaacgcttat
     5401 ggtcacggtc ttcttgagac cgcgcgaacg ctccccgatg ctgacgcctt tggcgtagcc
     5461 cgtctcgaag aagctctgcg actgcgtgcg gggggaatca ccaaacctgt actgttactc
     5521 gaaggctttt ttgatgccag agatctgccg acgatttctg cgcaacattt tcataccgcc
     5581 gtgcataacg aagaacagct ggctgcgctg gaagaggcta gcttggacga gccggttacc
     5641 gtctggatga aactcgatac cggtatgcac cgtctgggcg taaggccgga acaggctgag
     5701 gcgttttatc atcgcctgac ccagtgcaaa aacgttcgtc agccggtgaa tatcgtcagc
     5761 cattttgcgc gcgcggatga accaaaatgt ggcgcaaccg agaaacaact cgctatcttt
     5821 aatacctttt gcgaaggcaa acctggtcaa cgttccattg ccgcgtcggg tggcattctg
     5881 ctgtggccac agtcgcattt tgactgggtg cgcccgggca tcattcttta tggcgtctcg
     5941 ccgctggaag atcgctccac cggtgccgat tttggctgtc agccagtgat gtcactaacc
     6001 tccagcctga ttgccgtgcg tgagcataaa gccggagagc ctgttggtta tggtggaacc
     6061 tgggtaagcg aacgtgatac ccgtcttggc gtagtcgcga tgggctatgg cgatggttat
     6121 ccgcgcgccg cgccgtccgg tacgccagtg ctggtgaacg gtcgcgaagt accgattgtc
     6181 gggcgcgtgg cgatggatat gatctgcgta gacttaggtc cacaggcgca ggacaaagcc
     6241 ggggatccgg tcattttatg gggcgaaggt ttgcccgtag aacgtatcgc tgaaatgacg
     6301 aaagtaagcg cttacgaact tattacgcgc ctgacttcaa gggtcgcgat gaaatacgtg
     6361 gattaatcgt tctgtaatat ttgattgtct gtgccggatg cggcgtgaat gccttatccg
     6421 gccaataaaa tcctaaaaat tcaataagtt gatgttcttt catgctctta taaaggtcgt
     6481 gcctctggcg gatgtacgtt tatcattagt ctcactctgt tgctaattgc cgttcgctcc
     6541 tgaacatcca ctcgatcttc gccttcttcc ggtttattgt gttttaacca cctgcccgta
     6601 aacctggaga accatcgcgt gtttcaaaaa gttgacgcct acgctggcga cccgattctt
     6661 acgcttatgg agcgttttaa agaagaccct cgcagcgaca aagtgaattt aagtatcggt
     6721 ctgtactaca acgaagacgg aattattcca caattgaaag ccgtggcgga ggcggaagcg
     6781 cgcctgaatg cgcagcccca tggcgcttcg ctttatttac cgatggaagg gcttaacagc
     6841 tatcgccatg ccattgcgcc gctgctgttt ggtgcggacc atccggtact gcaacaacag
     6901 cgcgtagcaa ccattcaaac ccttggcggc tcaggggcat tgaaagtggg cgcggatttc
     6961 ctgaaacgct acttcccgga atcaggcgtc tgggtcagcg atcctacttg ggaaaaccac
     7021 gtagcaatat tcgccggggc tggattcgaa gtgagtactt acccctggta tgacgaagtg
     7081 actaacagcg tgcgctttaa tgacctgttg gcgacgctga aaacattacc tgcccgcagt
     7141 attgtgttgc tgcatccatg ttgccacaac ccaacgggtg ccgatctcac taatgatcag
     7201 tgggatgcgg tgattgaaat tctcaaagcc cgcgagctta tcccattcct cgatattgcc
     7261 tatcaaggat ttagtgccgg tatggaagag gatgcctacg ctattcgcgc cattgccagt
     7321 gctggattac ccgctctggt gagcaattcg ttctcgaaaa ttttctccct ttacggcgag
     7381 cgcgtcggcg gactttctgt tctgtgtgaa gatgccgaag ccgctggccg cgtactgggg
     7441 caattgaaag caacagttcg ccgcaactac tccagcccgc cgaattttgg tgcgcaggtg
     7501 gtggctgcag tgctgaatga cgaggcattg aaagccagct ggctggcgga agtagaagag
     7561 atgcgtactc gcattctggc aatgcgtcag gaattggtga aggtattaag cacagagatg
     7621 ccagaacgca atttcgatta tctgcttaat cagcgcggca tgttcagtta taccggttta
     7681 agtgccgctc aggttgaccg actacgtgaa gaatttggtg tctatctcat cgccagcggt
     7741 cgcatgtgtg tcgccgggtt aaatacggca aatgtacaac gtgtggcaaa ggcgtttgct
     7801 gcggtgatgt aatgcaggaa agcaggctgg agttacccag cctgcagtga aattaaactg
     7861 tcgtcgcttt cactatttct ttgtagatga tttttttgat gccgttgttc tacgtgagag
     7921 ataataaaac gttgtcagtt cttttattgt taagtttatc ccaattatct ggaattcctt
     7981 tccctatttt ttcgtgtgga gtaataattt ctgcacatct aaccattatt aaatttaata
     8041 ataagtatcg ggtttgttac gatagaatat ttctatatcc tcaagaatat gctttgcctt
     8101 attgcctgtg actttctgag attcaattat agtatcgtta aattctaatg ttaaagagaa
     8161 ctcttttttc cgctttgtgt aagtttaacc ttatattgat taccaatatc aaataaaaag
     8221 gtcctgtaat gagaataatc acctttaacc tcctcggcaa aacagcaaag cgtgcaaata
     8281 tacataaagt ctttcttacc cctgattttg ttaactaaat caataaatgc gtgaaaaact
     8341 ttacttgcaa tagaacttga tacttcttct ccaggtctac aaaaatcttt tgcaattttt
     8401 aactgtgttt ctatgtataa aaagaaatct ttggcataag agatatcttt attatttagt
     8461 gcatctagct caacaggtaa agatgtcgtt ttgttgctat tcacatatga tatattcata
     8521 tcatcatctc catcgcgagt aataaaatta atcaccattg tagggtaggg ggctggtcaa
     8581 tcagaaatca tctttataaa cttcgattgt ttttgtaatg ctgtatcatt aagttcatta
     8641 aatcgtacag cagataaatg ttctatcaaa tttcgctcat ttgccga
//
LOCUS       DAZOZX010000150         8679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZOZX010000150 DAZOZX010000000
VERSION     DAZOZX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.150"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(42..134)
                     /locus_tag="ACHUPT_003490"
                     /pseudo
     CDS             complement(42..134)
                     /locus_tag="ACHUPT_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHUPT_003491"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHUPT_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0820914.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACHUPT_003492"
     CDS             complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACHUPT_003492"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0820915.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACHUPT_003493"
     CDS             complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACHUPT_003493"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0820916.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACHUPT_003494"
     CDS             complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACHUPT_003494"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0820917.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5304..7850
                     /gene="cyaA"
                     /locus_tag="ACHUPT_003495"
     CDS             5304..7850
                     /gene="cyaA"
                     /locus_tag="ACHUPT_003495"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0820918.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACHUPT_003496"
     CDS             complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACHUPT_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0820919.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8258..>8679)
                     /locus_tag="ACHUPT_003497"
     CDS             complement(8258..>8679)
                     /locus_tag="ACHUPT_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR0820920.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREGNVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1975 a         2241 c         2235 g         2228 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg
     2101 ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca
     2161 gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact
     2221 ggtccacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta
     2281 cccaggtgga gacgttctcc agcgcctggc gataagtctc ttcctgatga cgcggtacag
     2341 cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg
     2401 gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa
     2461 agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt
     2521 caccgtctga atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta
     2581 cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg
     2641 cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat
     2701 tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct
     2761 gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc
     2821 aggttttagc atcgctgccg gaaatggtgg cgatcttttg ttggacttca tccaactgtt
     2881 ttgccagcgt ttcttgctga cgattcgcct gcttaagctg cgcagcttgt tgcttaataa
     2941 tgccttccag ctcggctttt tggctctcct gggctttttg caatgccgtc agttggttag
     3001 ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac
     3061 cgacacccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat gcagtattgt
     3121 tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct
     3181 cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt
     3241 attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc
     3301 agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta
     3361 gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac
     3421 cgctggtaac aacgaccatc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac
     3481 catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgca
     3541 ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt
     3601 tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct
     3661 gtgggtagag aatcttctgt ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa
     3721 tggcgaaata agtaggtagt tggggccatt tacgatcttg ctgatgcagc tgtgattggg
     3781 caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca
     3841 gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc
     3901 aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc
     3961 gggtgaccag gatactcatg ccggggcctc tccgttatag acttcagcga ggatctcgcg
     4021 cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg
     4081 cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca
     4141 tagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc
     4201 ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt
     4261 cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc
     4321 accttgtcct accgccggaa gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc
     4381 cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc
     4441 tagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg
     4501 gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg
     4561 taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc
     4621 gcaaatagtg accagcccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg
     4681 tacggcgata tcagcgcgat tctccaggag cgcgacttcc agctctttta caaataagcc
     4741 ttttccgcct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg
     4801 taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc
     4861 ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct
     4921 tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt
     4981 tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac
     5041 aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat
     5101 gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca
     5161 ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca
     5221 tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg
     5281 ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg
     5341 atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac
     5401 aggtctacag tctactgccg acattgttgc actatcacca tccgcgaatg ccgggttacc
     5461 ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact
     5521 acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc
     5581 ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct
     5641 ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttac
     5701 tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct
     5761 tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag
     5821 attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc
     5881 tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg
     5941 actatgtgat gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg
     6001 gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca
     6061 agagtatcga ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct
     6121 gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg
     6181 agattgtatc gtttggcctc gatccatact gcatgatgct ggagcgtgtt actgaatacc
     6241 tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag
     6301 tgtgcgaaaa actcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc
     6361 agttagtgag cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta
     6421 actggaagat tgatcaggtg cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga
     6481 gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc
     6541 aggatatcgg cgtactgacg cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag
     6601 tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt
     6661 atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata
     6721 ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg
     6781 cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg
     6841 gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc
     6901 tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg
     6961 cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt
     7021 ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta
     7081 gcgttgacct gctgtaccgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg
     7141 agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac
     7201 cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta atccgtactc
     7261 gcgtgcagca actggtttct gagtgtattg aactgcgcct ttccagcacc cgccaggaaa
     7321 ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc
     7381 tgaatgtatc ggtacagaag ctggagaacg ccatcgagtt ttatggcgct atttcgcata
     7441 acaaactgca cggcttgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg
     7501 tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagacgaga
     7561 atggctttaa tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg
     7621 aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc
     7681 gcttcaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga
     7741 aggttgatgg tcgcgaacag gtgattccgt tccgaacaaa atcgattggc aacctgccgc
     7801 ccgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga tgagcgtgcc
     7861 ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgt
     7921 tgcgtcgccg cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccat
     7981 tcatcgcctt tcaggtcaaa atggtagccg ccctgtttgg ttgccagcca cacctggtgc
     8041 agcggctcct ggcggttaat aatgattttg ctgccgtttt caaaggtaat ggtcagtacg
     8101 ccgccgttga tttcgcagtc gatatcgctg tcgccatccc tgtcgtccag acgctcttca
     8161 atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc
     8221 tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct
     8281 ctgctgctgc gttccggcat gattcatccg tcaccggaat aatgatgtct ctgttttgaa
     8341 cttcacgtac gttcccctct ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc
     8401 ttacctctgt atcggtccat gtataaacat tgcgatcgtc ataagcgaag cgatcgtttt
     8461 tagttagcga accgttaact ggaataattt gcaagctctt tgaccgtaat tggcggataa
     8521 agtaagcatg ctgtttgccg ctattaaatg agcgatcaat gaccaggtct ggcgtatcca
     8581 gcgggattat ctcaagctgg tcttcaccaa atgtcgataa ttttgttaca tacagccgat
     8641 ctttatattg cgcaatattg ccagtaatca gatggaatt
//
LOCUS       DAZOZX010000151         8562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZOZX010000151 DAZOZX010000000
VERSION     DAZOZX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.151"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(64..366)
                     /locus_tag="ACHUPT_003498"
     CDS             complement(64..366)
                     /locus_tag="ACHUPT_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820921.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(363..989)
                     /locus_tag="ACHUPT_003499"
     CDS             complement(363..989)
                     /locus_tag="ACHUPT_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFR0820922.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            complement(1177..2448)
                     /locus_tag="ACHUPT_003500"
     CDS             complement(1177..2448)
                     /locus_tag="ACHUPT_003500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFR0820923.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(2448..2885)
                     /gene="umuD"
                     /locus_tag="ACHUPT_003501"
     CDS             complement(2448..2885)
                     /gene="umuD"
                     /locus_tag="ACHUPT_003501"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0820924.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(2882..3130)
                     /locus_tag="ACHUPT_003502"
     CDS             complement(2882..3130)
                     /locus_tag="ACHUPT_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0820925.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            3549..4451
                     /locus_tag="ACHUPT_003503"
     CDS             3549..4451
                     /locus_tag="ACHUPT_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0820926.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIIDNVAHYGG"
     gene            complement(4402..4632)
                     /locus_tag="ACHUPT_003504"
     CDS             complement(4402..4632)
                     /locus_tag="ACHUPT_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFR0820927.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLSEKKRAVARPSPSYFIRHNAPHCRLSTQVR"
     gene            4548..4760
                     /locus_tag="ACHUPT_003505"
     CDS             4548..4760
                     /locus_tag="ACHUPT_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820928.1"
                     /translation="MNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDERPSSR
                     FRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            4836..5519
                     /locus_tag="ACHUPT_003506"
     CDS             4836..5519
                     /locus_tag="ACHUPT_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0820929.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            5520..5741
                     /locus_tag="ACHUPT_003507"
     CDS             5520..5741
                     /locus_tag="ACHUPT_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820930.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            5671..6189
                     /locus_tag="ACHUPT_003508"
     CDS             5671..6189
                     /locus_tag="ACHUPT_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0820931.1"
                     /translation="MAPIACSWKRIPKFWNWIPASKIEKETRMYGTCETLCRALAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGAAM
                     EIINNVRENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQA
                     RALLKNNTREND"
     gene            6234..7004
                     /locus_tag="ACHUPT_003509"
     CDS             6234..7004
                     /locus_tag="ACHUPT_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820932.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPSRRWFDRDILREVVLEARTEIRSMADALAKIRK"
     gene            7420..7845
                     /locus_tag="ACHUPT_003510"
     CDS             7420..7845
                     /locus_tag="ACHUPT_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0820933.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            7892..8314
                     /locus_tag="ACHUPT_003511"
     CDS             7892..8314
                     /locus_tag="ACHUPT_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0820934.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
     gene            8311..8502
                     /locus_tag="ACHUPT_003512"
     CDS             8311..8502
                     /locus_tag="ACHUPT_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0820935.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT         1891 a         2248 c         2351 g         2072 t
ORIGIN      
        1 agataacaag ataacaaggt gttatcttgt tatctcaagg tgttatcttg ttatctcaag
       61 gtgttatctt tgttttactt ctgctatcaa tcgtgatacc tctgtttttg atagtgtttt
      121 aagtgcttct accgccgccc tgacaacatc agaacgattc actctgcctt tacctgaaac
      181 catctcagcc gttataaggg catcaatgtc ttttaggttt tcttcaaaaa aagaaatact
      241 cactggtttt gctttgctta caaccgatga tttatctgat gactttttgg tattatcaag
      301 gccagccgtg ccaccgttaa taaatgctgc ctcgtcacga ggtgaagtgt ttgctctatt
      361 tctcacagaa taacctcttt cactaacgct tcgatttctt gctgggcttt atcattttgc
      421 gtttcaaaaa ttgtcattga ttcggcccaa gcatctcgat gtgctttacg atcacatacc
      481 cgggagtttg ccagatgcat ttcagggtaa tccttaagta cttcagctgc ctcattggct
      541 tcattaacaa acatatttgt gggcgtcatg tttaaaacga gatatccccg aacatcctca
      601 ttaaaatctt ttgctgctgt aaaaacggag catgtatgag gcacaacatc caggtccatc
      661 tgagatgggc gcaaaggaga aatgaataca tctgcggcca tcaatccact acgcatttct
      721 gcgctatcac ggccagcaca gtctgcaata acaaaatcat aggatttttc gtgttccttt
      781 aacatggctt tgatgttacc tgtcgcgcct gtaaccggaa tatgagggag atcttcaggg
      841 cgattgttgt accaggttaa tactgatttc tggtcatctg cgtcgacaat aatcactcta
      901 ttgcctagtg acatcagata cgcagctata gataccgcca gtgtactttt acctacccca
      961 cccttttgac tacctaatac tattatcatt atagcctcat attaagctgt taagttaaca
     1021 acttaatatt gccatctgaa cgcgaaccat gcaattattt ttgttatgtt aacaacttag
     1081 caagttgtta agtatttgat ttatagatta attaagataa caagataaca agataacaag
     1141 ataacaggat agcaatatta tggatggatt acatattcat aactgggcta ctggcaaatc
     1201 tgtccatctg gtggtatacg caggagaaag catttcccgt ttcatttgcc attcaggggc
     1261 ggttcctcgc cccgcaaacc acactttccc ttttcccgac tggttcagtt catcgagtgt
     1321 tttcattaac ttttcactgt ttttacgggg ctggatttca tcaaataatc ccggctgcgc
     1381 tatacccgat ggtgtgaaat cagccagcat gacacctgcc ttcatatagc ggtacccttc
     1441 acgccagaca tggtttaacg ctctgcatgc tgcggcaata atgtcccggc tgtcctgtgt
     1501 gggcaatgga agcttttcca cagcggcatt gctgtaacag ggttctttta ctgcaaaggg
     1561 ggatgtccgt acaaatgtcg tcacctgccg gcaatactga cgctccccac gtagtttctc
     1621 tgcggcccgc tctgcatact gaacaacagc ctggtgcatg gcatctttgt ctgtgattcg
     1681 ttcaccaaaa ctgcgactac agacaatctg ctgttttgcc ggcggtgctt cttccagaga
     1741 tatgcaggac tcgccgttga gttcgcgtac cgtacgctca agaatgacgc tgaagttttt
     1801 ccggatgaat gccgtgttag cctgcgccag ctgcagtgct gtgttaatac ccagcgcatt
     1861 cagcttttcc gtcagtctgc gtcctactcc ccagacctca ccaactggct gcaaccccag
     1921 tagcttcaag atccgattac ggttttctgc cgtcagcgcg accacaccgg aaaactgtgg
     1981 ccattgcttt gttgcccact gggcactttt agccagcgtt tttgtaggcg caatgcccac
     2041 ccccatggtg agtcctgtcc agctctttac ctgttccctg agctgatgac caaaaatctc
     2101 cggagagatg caatgattta tcccccgcaa atcaatgaac atttcatcaa ttgagtaggg
     2161 ctcaactgcg ggagaaagcg actccagaac agccataacc cgttggctca tgctgtggta
     2221 cagcgcataa ttgctggaaa atacatgtat tttcttctcc aggcgcattt gtctcacctg
     2281 aaaccagggc tgccccattc tgatgccaag ggcttttgcc tccgggctgc gcgcgatcac
     2341 acagccatcg ttattgctga gtacgatgac cggttcgttg cgaaggtccg ggcggaaaac
     2401 tttttcacat gaggcgtaga aactgttgat atcagccagt gcaaacatca gcgtaactcc
     2461 cgcgtcctgt gtatcacgtg agtgacaaca ccaaaaatac agatgttttc cggatacagt
     2521 gtgcggaact ccgggctgtc tgaaaccggc tccagtgccg ggcgtgggcg caacagcagt
     2581 cgtttgacgg tgaactcacc gtcgatctca gcgataacga tgtccccgtg ttgtggtttt
     2641 tcggccctgt ccactaccag cagatcacca ttctgcacgc cagcctggtt catcgattca
     2701 ccgctggcgc gcagaaagaa ggtggctgca ggtctgctga tgcaatagct gttcagatcc
     2761 agttcctgct cagcataatc agtggcaggc gaaggaaaac cggcctggca acgatcggca
     2821 aacaacggtc gcacgtaact gtcatcgcct gaaggggcag ccggacggtg ataaacggta
     2881 ctcatgctgc aggcagccag ctgtcgtcct gccagatttc ttccagcaga gacaggatac
     2941 gttctttatc cttgtcgttg cgggctcctg tgacagatac agacggggcg ctgccctggt
     3001 ctatacgcag ccagatttcc gggtagtgtg gtttcagacg ttgttcgatt tcattctgta
     3061 gcgcctgaag tgtgcctgat ttgaggtttt ttgtgctctg gcggtcgaaa agaatttcaa
     3121 tgcggatcat gttctcatct cccttttctt tatgctgtat gtttatacag tattctaata
     3181 actgaatccg cacacagtca aatgtcattg ttgtgactaa aaaacgctca attctctgag
     3241 tgtgtggctg tatgcctgct gtgggctgct gtgtccagct tatccacagc attttgtgca
     3301 cggttctgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa ccgggctgca
     3361 tccgatgcaa gtgtgtcgct gtcgccggcc tcctcacccg gtcacgtttc gtcgttcctc
     3421 ctccacgcgc tgcggcttcg gggccgcacc tgcattcgta tgcggtcgcc cggtaacagg
     3481 tgcggcacgg cctgatggag gccgcatgtg agaggagaat tcccatgcca aactggtgct
     3541 cgaatcgtat gtatttttct ggtgaaccgg cacagatcgc tgagattaaa cgactggcca
     3601 gcggtgcagt tacaccgctt tatcgccgcg ccacaaatga aggtattcag ctgtttctgg
     3661 ccggaagtgc cggacttctg cagatcactg aaaatatccg ctctgagcag tgccccggag
     3721 tgacggctgc cggacgtggt gctgtatcac cggaaaatat cgcgttcacc cgctggctga
     3781 cacacctgca gaacggtgta ctgctggatg agcaaaactg cctgatgctg catgaactct
     3841 ggctgcagag cggtactgga cagcgtcgct gggaagagtt accggatgat gtcagggaaa
     3901 ctatcactgt gcatttcacc gcaaaaagag gtgactggtg tgacatctgg ggtaacgagg
     3961 atgtatcggt gtggtggaac cgtctgtgtg acaacgtact gccggaaaaa accatgccgt
     4021 ttgacctgct gacggttttg ccgacccgcc tggatattga agtgaatggc tttaacggcg
     4081 gtgttctgaa cggtgttcct tctgcatatc actggtatac agaacggtat ggcgtgaagt
     4141 ggccttgtgg gtatgacctg aatatcagca gtcaggggga aaacttcatt caggtagatt
     4201 tcgacacgcc gtggtgtcag ccggaaagcg acgttgttgc agaattaagc cgccgtttcg
     4261 gctgcacgct ggagcactgg tacgccgaac agggctgcaa tttctgtggc tggcagttgt
     4321 atgagcgcgg agagctcgtt gatgtgctgt ggggggaact ggaatggtct tccccgacag
     4381 atgatgatga gctgccggaa gtcaccggac ctgcgtggat aatcgacaat gtggcgcatt
     4441 atggcggatg aagtatgacg gagacgggcg ggcaaccgcc cgtttctttt ccgacaaagg
     4501 atgtcgccgt gctcctcttt ttactgcgcc ggctgacgcg gcgcggcata aacgctgcct
     4561 gtggtcagtg tcccgcgtcc ggccgggcac gggacgggcg cttttaccgc tcccggctgg
     4621 cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga tgaacgccct tcaagccgct
     4681 ttcgcggcat agccttgccg tcaaaaagac gacggctgcg gtattccacg gtcggcctga
     4741 cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcacc acaggggtgc
     4801 cctcccggtg ccctctgtaa gagaaggagt ttggtatgtc ccgttttgtc ctcggtaact
     4861 gcatcgatgt tatggcccgt atccctgata acgccattga tttcatcctc accgacccgc
     4921 cgtacctcgt cggttttcgt gaccgtcagg ggcgtaccat tgccggcgat aaaaccgatg
     4981 aatggctgca accggcctgt aatgagatgt accgcgtact caaaaaagac gcgttaatgg
     5041 tgagcttcta tggctggaat cgcgtcgatc gttttatgtc tgcctggaaa aatgcgggat
     5101 tcagcgttgt tggtcacctg gtcttcacca aaaactacac atcgaaggcc gcatatgtgg
     5161 gctatcgcca cgaatgtgcc tacatcctgg caaaaggccg tccacgtctg ccacaaaacc
     5221 cgctgccgga cgtgctgggc tggaaatatt cgggcaatcg ccatcacccg acggaaaagc
     5281 ccgttaccag cctgcaacca ctgattgaga gcttcacaca tccgaacgcg attgtgctgg
     5341 acccgtttgc aggcagcgga tcaacctgcg tggccgctct tcagtccgga cgccggtata
     5401 tcggtatcga gctgcttgaa cagtatcacc gtgccggaca gcaacgactg accgccgtgc
     5461 aacgggccat gcagcagggg gccgcgaatg ataactggtt tgaaccggag gctgcgtaaa
     5521 tgaactatgc cggacacgaa aaacttcgcg ccgacgtggc ggaggtggcc aacaccatgt
     5581 gtgaccttcg ggccaggctg aacgatatgg agcatcgctg tcgctttgat tccgatgtgc
     5641 ttgttgaacg cctgacacgc cagacgctgt atcgcgccaa tcgcctgttc atggaagcgt
     5701 ataccgaaat tctggaactg gattcctgct tcaaagattg agaaggagac gagaatgtac
     5761 ggaacatgcg aaacgctctg ccgggcgctg gcagcaaagt atccgggaga tacgccgctg
     5821 atgctggtta tctggtcccc ggaagaaatt caggccctcg ctgacggaat ggatatttcc
     5881 ctgtccgatc atgaaatcag gactgtcctg gcgcgcctgg aggacatccc ggaagaccag
     5941 cggactgaat ccggtatttc ttccggtgcg gcgatggaga tcatcaataa cgtgagagaa
     6001 aaccgccagg tgaccgtccc tgctgaactg ctggcgtccc tgattcagac cgccgaacag
     6061 gcactgtgga aacgtgagtg ggccgcccgt gataacggcc tcgccgtccc ggaatgcgtc
     6121 acccgccgac aggcggtggt taatcaggcc cgcgccctgc tgaaaaacaa cacacgcgaa
     6181 aacgactgat gttatcgccg cctgcgggcg gcgattcagg gagaccatga atcatgaacg
     6241 aaacgctgaa tgcactgatt tgtcgccacg cccgtaacct gctgctggcg cagggctggc
     6301 cggaagagac ggatgttgac cagcgtaacc cgaagtatcc gggctggatc agcatttatg
     6361 ttctgctgga tgcgccccgg ctggcgacgt tacttatcaa ccgtcacggc ggcgtactgc
     6421 cgccgctcct ggcctctgcc attcagaaac tgaccggaac cggggcggaa cttgttctgt
     6481 ccggcagtca gtggcagtcg ctgccggtac ttccggcaga cggaacgcag gtgtctttcc
     6541 cgtatgccgg agaatggctg accgaagacg aaatcagggc agttcttgat gcggtgcacg
     6601 atgcggtacg cagtgtcagt tgccgggtgg cagaagatgc gcggcgtatc agggcggcgc
     6661 tgaccacaac cggtcagacg ttgctgaccc gccagacgcg ccgctttcgc ctggttgtga
     6721 aggaaagcga tcacccctgc tggctcgatg aagacgacga aaacctgccc gtggtgctcg
     6781 atgccatcct gaaccggggc gctcgttttt cgtcggtgga aatgtatctg gtcagtgagt
     6841 gtgtggagca tatcctgtcc agtgggctgg cctgcgatgt gctgcgtata cctgatgaac
     6901 cgtcccgtcg ctggtttgac cgggatattc tgcgggaggt ggtcctggaa gcccggactg
     6961 aaatccgcag catggcggat gcactggcga aaatccggaa atgatgacgc tggcggggaa
     7021 aaatctccgc cttttccagc cggtccccgg aaagagcaaa aggatgtcgc cgcaggctcc
     7081 gctttacccg gccatgcggg gcatggcctt gtgggttttc agttatgtgg cctcagcgcc
     7141 gtgtgcgggc tgtgccgtgc ctccatcgta gccgggcttc tgcgggcggg ttgcaaggta
     7201 aggtgaaccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt
     7261 cacccgctca gatttcgcgg tctcaccctt caactcccgt cagccgtgcg cgtcaggctg
     7321 cggcttccct tgcgcccttg caaccctgca ggcccggcaa agatggaggc acggcaccgc
     7381 ccggtgtcgc ggaatatgtg aacaacggag gaaaacatca tgcaatatgc gaaacctgtc
     7441 actctgaacg ttgaagagtg cgaccgtctg tcttttctgc cttacctgtt tggcctggat
     7501 tttctgtatg ccgaagcgtc tgtatacgcg ctggcgaaaa aaatgatgcc ggaatatgaa
     7561 ggcggattct ggcacttcat ccgcctgccg gacggtggcg gttacatgat gccggatggc
     7621 gaccgtttcc acatggtgaa cggtgcaaac tggtttgacc gtaccgtgag tgctgatgcc
     7681 gcaggcatca tccttacctc ccttgtgatt aaccgccagt tgtggctgta ccacgacagc
     7741 ggggatgcag ggttgacaca cctgtacagg atgcgtgatg cgcagttgtg gagccacatc
     7801 gaatttcacc ccgaatgcaa cgcgatttac gcggcacttg actgattaac ggacggggcg
     7861 gcaatgccgc ccctgtaaag gagacgagaa catgtactgt actgttaaag aaatcatccg
     7921 cgatgtactg gatacagacg tgccggacag tgaatgcgtt tttgccgtgg tgctgacccg
     7981 tggggatgtg cgtcacatag cccaggactg gaacctgtca gacgatgagc tggaaaccgt
     8041 catgcagcgg ctggacgatg cttttgtata tggcgcatgt gacagagttg tttccgatat
     8101 tgtcaatgaa ctgatggaag aaaagcgcgc cagccgtcag gtgacagtcc cggcggtgat
     8161 gctggaaaaa gtgatggcgc tggcaggcag tgaaatgaag cgcctgtatg ccgtcgggag
     8221 tgagaacggg ggcgacggtg acgcattcgt cagggaggaa ctcgaagcaa tggacgttgt
     8281 gttgcaggcg ctggacgggg agacgatgtc atgaatatca gcacagaaac ccgcgaaatt
     8341 ctgcgtaatt acagggccgt gattaatgcg cggcgtcgtg aaatggggca gaaaccgctc
     8401 accaccgcgc agattgttga tgaaatctgc gattttgtgg cgaatcagca ggcggttttc
     8461 ctgggcggtc actacatcct tcagggcagc agaaacaggt gatacagggg caggcggtgg
     8521 aggccgcctg tcagcccgcg cgaaaacccc tggcaggctg ac
//
LOCUS       DAZOZX010000152         8528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZOZX010000152 DAZOZX010000000
VERSION     DAZOZX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.152"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..189
                     /gene="csgC"
                     /locus_tag="ACHUPT_003513"
     CDS             <1..189
                     /gene="csgC"
                     /locus_tag="ACHUPT_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0820936.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            310..621
                     /gene="ymdA"
                     /locus_tag="ACHUPT_003514"
     CDS             310..621
                     /gene="ymdA"
                     /locus_tag="ACHUPT_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0820937.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            716..1249
                     /gene="ymdB"
                     /locus_tag="ACHUPT_003515"
     CDS             716..1249
                     /gene="ymdB"
                     /locus_tag="ACHUPT_003515"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0820938.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1191..2672
                     /gene="clsC"
                     /locus_tag="ACHUPT_003516"
     CDS             1191..2672
                     /gene="clsC"
                     /locus_tag="ACHUPT_003516"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0820939.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHUPT_003517"
     CDS             complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHUPT_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0820940.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHUPT_003518"
     CDS             4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHUPT_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0820941.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHUPT_003519"
     CDS             5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHUPT_003519"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0820942.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2222 g         2131 t
ORIGIN      
        1 ggcagttcag ggcaaagtca gacgaagcaa gaaaagaccc tctcattgcc tgctaatcaa
       61 cccattgctt tgacgaagtt gagtttaaat atttccccgg aagatcgggt gaaaatagtt
      121 gttactgttt ctgatggaca ggcacttcat ttatcacaac aatggacgcc ctcttcagaa
      181 aagtcttaat ttgttgaaat atcgagcata agatgaatct ggagagaatg gtctgctgcg
      241 aatcagccaa cctgaaagta tggataacac aaccttcaag gatgactaat cattgaggaa
      301 atagaataaa tgttcagacc ttttttagac tctcttatgc tcggcagttt gttttttcct
      361 tttattgcca ttgcaggaag caccgcgcaa gggggcgtga tccattttta tggccaaatt
      421 gtggaaccgg catgtgacgt cagcacccag tcatcacccg tagaaatgaa ctgcccacag
      481 aatggttcta ttccgggcaa aacctactcc agcaaagcgt taatgagcgg caatgtcaaa
      541 aacgcgcaaa tagcctcagt aaaagtgcag tatcttgata aacagaaaaa gctggctgta
      601 atgaacatcg aatataacta agtttctgaa caactcacgt tgctgagcag aaaaatgcga
      661 tttaaccaaa aagcctgctg tacacttaag aaacaagaag gtgtaaggag gcgttatgaa
      721 aacgcgtatt catgttgtgc agggtgatat taccaaactg gccgttgatg tgattgtgaa
      781 tgcggctaat ccgtcattaa tgggaggcgg cggcgtcgat ggggccattc atcgcgcagc
      841 gggtccggcc ctgctggatg cctgtttaaa agtcaggcaa cagcagggcg attgccctac
      901 ggggcatgcc gttattacgc ttgcaggcga tcttcccgct aaagccgtag tacacaccgt
      961 cgggccagtc tggcgtggtg gggaacaaaa cgaagaccag cttttgcagg atgcctatct
     1021 gaatagccta cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag
     1081 tactggggtt tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc
     1141 agaatttatt acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga
     1201 aaacgcccac ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc
     1261 tggcgagcgc ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc
     1321 tggagaaaag tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata
     1381 cgctggatgt tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg
     1441 ccctgttagc cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata
     1501 cgcccggact tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc
     1561 tttttaatcc tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc
     1621 gtctcaatcg ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg
     1681 gaggacgaaa tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt
     1741 tagatgtcat ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact
     1801 ggtattgcaa atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg
     1861 cggatcgcat cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac
     1921 gcaaaatgga atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct
     1981 gggcgaagac acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc
     2041 attcactgtt accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata
     2101 ttatctcttc ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga
     2161 gaaaaggggt aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg
     2221 tccatgccgg atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg
     2281 aactcaagcc gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt
     2341 ccggtgccag cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct
     2401 ctttcaattt cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga
     2461 gcgaaacgct ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct
     2521 ggcagctccg tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag
     2581 aggttattct caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg
     2641 cgtcgatatt gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc
     2701 gttctcacgc ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa
     2761 atgaatttca tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc
     2821 aagccagttg gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg
     2881 gtgaaccaga tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa
     2941 gttaagcaaa cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat
     3001 ggtgatcacc gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag
     3061 taaataagcg acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa
     3121 caaggcttta agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata
     3181 aaatagcgtt tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac
     3241 aataaaaatc gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga
     3301 tagttttacc atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat
     3361 gcgcttaaat atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag
     3421 gtgtgatatt aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca
     3481 actttccgct ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg
     3541 gatggctgtt aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa
     3601 gggatagcgt aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat
     3661 gcggaacgag tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt
     3721 cacatgccat gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat
     3781 cagccaggcg cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata
     3841 tggttaacta atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt
     3901 gccatagcga caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca
     3961 tcatcgctac cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct
     4021 ggcaaaccct acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg
     4081 gttgcaatca ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt
     4141 cggaatggct ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag
     4201 tgcttactaa ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca
     4261 gtaatgttaa ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag
     4321 caagctcaat ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt
     4381 ttccgcgaca tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac
     4441 tggaacaatc tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat
     4501 accccggtca aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg
     4561 gattatttca ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt
     4621 gccggtttta aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc
     4681 atgctcgggg ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc
     4741 cgcggcctag caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag
     4801 ttctggatcg agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac
     4861 tcgccgcgtg cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg
     4921 gatgtgcagt cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta
     4981 accagtatgt tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag
     5041 ttgcacgact ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg
     5101 ttgaataacc cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt
     5161 ggtctgttgc aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat
     5221 ctccgtccaa gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg
     5281 gtggaaattc caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat
     5341 cagctgccgg agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat
     5401 gaagacaaac tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg
     5461 gatgtgaagc agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat
     5521 tttaccggcg cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc
     5581 attggtgata atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc
     5641 tggcgtctgg tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct
     5701 gcgctggtga atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat
     5761 gaataagaca actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt
     5821 gccgaagact gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc
     5881 gcgggaggat gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga
     5941 ttcacttgct gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat
     6001 gccagaagca aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg
     6061 tttctgggat cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa
     6121 agaagagcag gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct
     6181 gttgatcctg acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc
     6241 ttatcagggt tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc
     6301 ctttatgcag cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact
     6361 gttctgttgg gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat
     6421 tggtcgcgat aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga
     6481 gcatcgcacg gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg
     6541 cttgcgtgca acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta
     6601 cattctgagt gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga
     6661 gcttatcgct gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt
     6721 gaagcgtaaa agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta
     6781 catggtggtg ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg
     6841 cttgatggaa gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat
     6901 ggatacgctg tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac
     6961 agccggtttg cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat
     7021 ccgcgtgaaa ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt
     7081 tgccggttca atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg
     7141 gggggtctgg attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct
     7201 tgatgagcta aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt
     7261 cctggttaag ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta
     7321 tctctccgct ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca
     7381 tgcgttgacc gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca
     7441 gtggcgtcct gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc
     7501 gaagttattg agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg
     7561 gcgcgttaca ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg
     7621 catgctgttc catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa
     7681 ttcaccgcag cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc
     7741 acagctgctg ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct
     7801 gttctggctg gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc
     7861 cagccgtgcc accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga
     7921 gtattcaccg ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg
     7981 ctcccttgac gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac
     8041 cgcaatggcg acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca
     8101 cgttgaacag gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct
     8161 gctaagcgat ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag
     8221 atattcttca tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa
     8281 accggatgcg gcttcgcaat aaaaacgtag ttgcctgatg cgctacgctt atcaggccta
     8341 catcgttcct gcaatttatt gattttgcaa gattttgtag gtcggataag gcgttcacgc
     8401 cacatccggc aaaaacaacg agccaataaa aataccggcg ttatgccgta atgccagtca
     8461 gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc ctctccttta
     8521 ccactctc
//
LOCUS       DAZOZX010000153         8332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZOZX010000153 DAZOZX010000000
VERSION     DAZOZX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.153"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(168..566)
                     /locus_tag="ACHUPT_003520"
     CDS             complement(168..566)
                     /locus_tag="ACHUPT_003520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR0820943.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHUPT_003521"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHUPT_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR0820944.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..6128
                     /gene="traI"
                     /locus_tag="ACHUPT_003522"
                     /pseudo
     CDS             875..6128
                     /gene="traI"
                     /locus_tag="ACHUPT_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="ACHUPT_003523"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="ACHUPT_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR0820945.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="ACHUPT_003524"
     CDS             6949..7509
                     /locus_tag="ACHUPT_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0820946.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="ACHUPT_003525"
     CDS             7640..7852
                     /locus_tag="ACHUPT_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0820947.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8332
                     /locus_tag="ACHUPT_003526"
     CDS             8096..>8332
                     /locus_tag="ACHUPT_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0820948.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2670 g         1663 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggtgc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca ca
//
LOCUS       DAZOZX010000154         8298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZOZX010000154 DAZOZX010000000
VERSION     DAZOZX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.154"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHUPT_003527"
     CDS             <1..1117
                     /locus_tag="ACHUPT_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0820949.1"
                     /translation="KVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNKC
                     DDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIKD
                     EDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGTL
                     NACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSFS
                     KLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNFN
                     NANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTL
                     QKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1271..2920)
                     /locus_tag="ACHUPT_003528"
     CDS             complement(1271..2920)
                     /locus_tag="ACHUPT_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0820950.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3071..4420)
                     /gene="ghxP"
                     /locus_tag="ACHUPT_003529"
                     /gene_synonym="yjcD"
     CDS             complement(3071..4420)
                     /gene="ghxP"
                     /locus_tag="ACHUPT_003529"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0820951.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(5126..5590)
                     /gene="soxR"
                     /locus_tag="ACHUPT_003530"
     CDS             complement(5126..5590)
                     /gene="soxR"
                     /locus_tag="ACHUPT_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0820952.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5676..5999
                     /gene="soxS"
                     /locus_tag="ACHUPT_003531"
     CDS             5676..5999
                     /gene="soxS"
                     /locus_tag="ACHUPT_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0820953.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(6002..7579)
                     /gene="pdeC"
                     /locus_tag="ACHUPT_003532"
                     /pseudo
     CDS             complement(6002..7579)
                     /gene="pdeC"
                     /locus_tag="ACHUPT_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            8008..8289
                     /locus_tag="ACHUPT_003533"
     CDS             8008..8289
                     /locus_tag="ACHUPT_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR0820954.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2335 a         2101 c         1889 g         1973 t
ORIGIN      
        1 aaaagtgata ggcttgattc agaaaatgaa aagaatttat gatagtttac catcaggaaa
       61 aatcacgaaa gaaacggaca ggaaaataca taaatatttt atagatatag cgttatatgc
      121 aaataataaa tgtgacgata gaattacgag aagagtttac cttagtaaag aaaaggatgt
      181 atccattaag gtggtatatt ttataaataa tgtcgccgtc cataataata ctatcgaaat
      241 tccacagata gtaaatggtg gttacgattt ttcacacctt agcctgaagg gtatcgtgat
      301 taaagatgaa gatttatcca attcgaattt tgcaggttgc agactacaaa acgctatttt
      361 ccaggactgt aatatgtata aaacgaattt ttattacgcc ataatggaaa aaatactttt
      421 tgataattgt attctcgatg actcaaattt cgctcagata aaaatggccg acggaactct
      481 aaatgcatgc tccgctatgc atgttcaatt ctacaatgca gcaatgaata gagccaatat
      541 taaaaatacc ttccttgact attcaaattt ttatatggcg tacatggctg aggtaaatct
      601 ttataaagta atagcgccat atgttaattt atttaaagcc gaccttagtt tctctaaact
      661 cgatttaatt aactttgaac atgctgatct gtctcgcgtc aatctgaaca aagcaatcct
      721 ccagaatata aacttaattg atagcaaact cttttgtacg tggctgacaa atacattcct
      781 cgaaatggtt atatgtaccg gctctaatat ggctaatgtt aattttaata atgccaattt
      841 aagcaactgc catttcaact gttctatttt aacaaaagcc tgtatgttta atacccgtct
      901 ctatcgggtt aatttcgatg aggctagcgt ccagggaatg ggcatttcca ttctccgtgg
      961 ggaggaaaat atccccattg atagtgatac cctggtaaca ctacagaaat tctttgaaga
     1021 agattgtacc tctcatactg gcatgtcaca aactgaggat aatattaatg cagtcgctat
     1081 gaagattact gcagatatta tgcaacacgc agattgaggt taagtcgaag aacagccaat
     1141 gaattgactt tacaagagag ataacttatt tgttttagaa tcatgaatgt tcgcccgagc
     1201 taacctgtta aaggtgcaaa agattgcacc tttttcagat tacctcgggc ctggttacgt
     1261 cgctttcggc ttactgattt tcctcaatta gcaacgcttc aagcaaatcg agatcgtgca
     1321 gtaatttttg cagcgtttcg ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag
     1381 cacgttcaga acgcaatgcc gccagacgga agcgacgctc aaggttctct tcctgcacgg
     1441 aactttcaac gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg acgcgagaac
     1501 tgacctccgt aagcaactgg ttatcgatat tctcttcggt atccgccgcc agacgctcct
     1561 ccattttctg gatcgccaca atcgccactt ctgccgttgc cgctcgcgca atacgttctt
     1621 ctttcaattg ttgcgaatgg tccgcgactt caatgtgttg tagcagaata ggcaacatca
     1681 ccacgccgac aaacagcgag aagagaatga cgccagccgc caggaacacc agctcatagc
     1741 gcgccgggaa gacgttacca tccggcaaga gcagcgggat tgagagcaca ccggccagag
     1801 tgatcgcccc acgcacccca gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac
     1861 caaactccat cggcttcttc ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc
     1921 agccgaaacg gaccagcatc agcgccgcat atatcagaat aatatcggta aacagcatcc
     1981 agatttcgac gttagggtcg atttctgccg ccatcagcga cgtctccaga atacccggca
     2041 gttgcagacc taacagcagg aacaccatgc cgttaaagac aaattccagc atcgcccagg
     2101 tgctgtttgc gcgcaggcgc attgccagtg gcgcacggcg catcacaccg gagcgggtga
     2161 tggtcatccc ggcggcaacc gcagcgagga tgcccgaaac gccaatatgt tcggcaatca
     2221 gataagaagc gaatggcagc aagaacagca gaacgatctg cgtcgcgggt tcatcaccgc
     2281 cccagcggct gaggaatcgc agcgagcgac catacagcca gctcaccaca aaaccggcaa
     2341 gaataccgcc aatggctact ttcataaatt cgaccgtcgc accgccgaca gtgaagatca
     2401 tcgtccccat cgccactgcc acggcaaact tcaacgacac caggccggaa gcgtcattca
     2461 tcaacgcttc gccctgcaaa atgcccatga tttttttcgg gatgcgccct tcgccaacaa
     2521 tcccggagag cgccacagca tcggtcggag aaagtaccgc cgccagcgca aaggcgggga
     2581 tcagcggaat gcccggcacc acccagtaaa taaggaagcc aatgccgacc acggtgacca
     2641 ccaccagcgc cagcgcgagg ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg
     2701 gcgttttcca gccatcagcg aacagcaacg gcgggataaa caagactaaa aagagttcag
     2761 gatcaaactc cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg atggcgattt
     2821 gcataagcgg aagcgggatc tgaaagggca tgacacgagt gaccaccccg gacagcgaga
     2881 ccacgagggt cattatcagt atggtgaaga agatttccat gcgttccctg tttgcgatgt
     2941 tttttatgat ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa
     3001 gctcacacaa tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc
     3061 cgatgaaaga ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa
     3121 cgaccgtacc gatgttcaac ttgcgccatt caccggaaac cagacgacca atcaccagag
     3181 tcgcgaagcc gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg
     3241 taaccagacc cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca
     3301 gcatcagcag gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag
     3361 aaagcggaga gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaacc
     3421 cggttttacc gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg
     3481 cacccaccag gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccgccgt
     3541 cgatgatctg cccatcttta tccagcaggt tcgcctggcc ggcgacagcg cggatagttc
     3601 cggtggcatc aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat
     3661 tcagcgcgcc cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg
     3721 aaggcatggc gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg
     3781 agataccgat aatggtcagc agaatgccac cagggacttt cagtttttcc aagccgatga
     3841 tcaccgccag acctgccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa
     3901 cgggcagacc atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca
     3961 gcaggaacag accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca
     4021 aaatccagct acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca
     4081 cggcacccag cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa
     4141 acgccgtcag ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca
     4201 cgatagaacc gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc
     4261 ctgctttacc caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg
     4321 ttaacccggc aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg
     4381 cgtcgagtga accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag
     4441 tttaattttc gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg
     4501 ctccaggttt gcggcaattt ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac
     4561 aaaacgggtg gtaaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg
     4621 aagtttgctg cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg
     4681 caaacgttat cgtcgatgtg caagattgca gcgttatcga gccgtatacc gttctggatt
     4741 gcgataaaaa atcgtctgag atgacaaaaa tattgcggct aaaaagtata cttttacact
     4801 ggcgaagaaa cgtcggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac
     4861 gtgctcgaat gaggtgtgtt gacgtcgggg gaaaccctcc tgtgttccag cgggatagag
     4921 agaaagacaa agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgtgttt
     4981 gcggataatt ccaaaagagc tcgatcagtc ccctcgcccc ttcggggaga gggttagggt
     5041 gaggggaaca ggcagcacca gtgcaaatat ttaccctcac cccggcccca tagttaacga
     5101 tgagtcagct tgtccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgccgg
     5161 taccttcttc tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg cgcgaaaggc
     5221 agccacaacc aatacatccg tccagttcgt cacgcagcgc cactaaggta tgaatgcgcc
     5281 gatccaactc ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaacgtat
     5341 gcccttctgg caacacgcca aacgcttcac caatggtcgc cagcggaatg ccaatacgct
     5401 gagcgatttt gataattgcg acatatcgca acacatcacg tttatatcgc cgctgattgc
     5461 cgctgttacg gatactagta atcaaccctt tgctttcata gaaatgcagc gccgataccg
     5521 ccacaccgct gcgtttcgcc acttcgccag gggttagcag cgctttaatg cggggtaatt
     5581 tcttttccat aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg
     5641 aattaacgaa ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg
     5701 atcttatcgc atggattgac gtgcatattg accagccgct taacattgat gtcgtcgcaa
     5761 aaaaatcagg ctattcaaag tggtacttgc aacggatgtt ccgcacggtg acgcatcaga
     5821 cgcttggcga ttacattcgc caacgccgcc tgttactggc cgccgttgag ttgcgcacca
     5881 ccgagcgtcc gatttttgat atcgcaatgg acctgggtta tgtctcgcag cagaccttct
     5941 cccgcgtttt ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt
     6001 tttattgccc gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct
     6061 gcggcggcat cgccttcgca aaaaaccatc cctggcaata gcgcacgccg cgtttgcgca
     6121 gccagttaac ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccaggctgt
     6181 gcgccagctc gatgatgtgt tccgcaatca aatgactggt tttgtgggtg gtcagcgttt
     6241 caacaaacga tttatcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt
     6301 tagagtagcc aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca
     6361 gaattaatcg gcgtcatttt ttcgacatca agaaatgcat gctcagtcac ttcaaattta
     6421 atttgctgcg gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt
     6481 gacgtatgaa aatcggaggc cgacaggtta atagaaacat agcgatcggc atgtgttgcc
     6541 aggtacgcgc ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttctatc
     6601 atcccctcct tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca
     6661 ccaggccaac gtaacaacgc ttcagcgccg atacattttt ctgttttgat atcgattatt
     6721 ggctggtaat aaagacaaag ttgatgtttt tcgagggcgc gttgcaattt acgtttggga
     6781 gataaatagt tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata
     6841 ccggcgggca acgcaaaaat aagatgatta taaaaatggg ttataagacg ttgatatgaa
     6901 gtcgcaacaa tggctgcaat tgggcgtttt gtcgaataaa ttgtcgcata taaatagcca
     6961 tttctttgta cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc
     7021 tctttgctta acgagaaaaa ggttttcatc accgtatcat acacacccca ttgcaatgtc
     7081 gggtcatcag acatcacttc actccagaag agagggttga taaccgccac ataatttccc
     7141 cgctgcatat aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata
     7201 gaaacgttag gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct
     7261 atcagcgatg agcataaaaa atggttatca cgaattcatt aatataaaga tagccacgaa
     7321 taatattcaa cattcgcttt tgatgggctg gagtgcaaac ttgcccctga tagcgttcag
     7381 ctgcatcgct cactaaatct gcctggcgaa tgaccagctc ggatttatcc agtgccagtt
     7441 gagcaaaggt tcggagctga ttattcactt ctgattttgc ccaaaggaag gcaatccata
     7501 gcgaaagaat gatgggaaag agaactaaaa agataatgcc cggcaacgcc agtaattggt
     7561 gtcgtgcacg atgactcatt gctatcaatt tcctgcctgt ttatacccgg ccattgcaag
     7621 gtgaaaccct ggctaccgac gtctttagat aaatcttacg gtataaatag cacttttgcc
     7681 tgatttgaaa ttgatacctt tttaatcgtc acctggatga aaataattca ttgatattca
     7741 aaattaattt tctacaggaa tacagaggcg gcgggaagat atgcccttaa tgccactgaa
     7801 aaaccacata atttgtacag caataaaagt cacggcctaa tatggctaac aaacaatcaa
     7861 ttgtgatata gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca
     7921 agccccgcga tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc
     7981 atcggctctt ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt
     8041 cgctggcaac ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt
     8101 cgtcggtctg attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc
     8161 tgttggttcg caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac
     8221 aacatcgagt cggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt
     8281 ttgacctgat acgtagaa
//
LOCUS       DAZOZX010000155         8084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZOZX010000155 DAZOZX010000000
VERSION     DAZOZX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.155"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..98
                     /gene="yodD"
                     /locus_tag="ACHUPT_003534"
     CDS             <1..98
                     /gene="yodD"
                     /locus_tag="ACHUPT_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0820955.1"
                     /translation="SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            396..1211
                     /locus_tag="ACHUPT_003535"
                     /pseudo
     CDS             396..1211
                     /locus_tag="ACHUPT_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(1208..2902)
                     /gene="dgcQ"
                     /locus_tag="ACHUPT_003536"
                     /pseudo
     CDS             complement(1208..2902)
                     /gene="dgcQ"
                     /locus_tag="ACHUPT_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(3073..3255)
                     /locus_tag="ACHUPT_003537"
     CDS             complement(3073..3255)
                     /locus_tag="ACHUPT_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0820956.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(3334..4251)
                     /locus_tag="ACHUPT_003538"
     CDS             complement(3334..4251)
                     /locus_tag="ACHUPT_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0820957.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4424..5344
                     /gene="yedA"
                     /locus_tag="ACHUPT_003539"
     CDS             4424..5344
                     /gene="yedA"
                     /locus_tag="ACHUPT_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR0820958.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(5333..5803)
                     /gene="vsr"
                     /locus_tag="ACHUPT_003540"
     CDS             complement(5333..5803)
                     /gene="vsr"
                     /locus_tag="ACHUPT_003540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0820959.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(5784..7202)
                     /gene="dcm"
                     /locus_tag="ACHUPT_003541"
     CDS             complement(5784..7202)
                     /gene="dcm"
                     /locus_tag="ACHUPT_003541"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0820960.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(7269..7770)
                     /locus_tag="ACHUPT_003542"
                     /pseudo
     CDS             complement(7269..7770)
                     /locus_tag="ACHUPT_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(7849..>8084)
                     /gene="drpB"
                     /locus_tag="ACHUPT_003543"
     CDS             complement(7849..>8084)
                     /gene="drpB"
                     /locus_tag="ACHUPT_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0820961.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         1929 a         2213 c         1982 g         1960 t
ORIGIN      
        1 ttagcgtcga tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg
       61 atattcatga cttcagcgtc tctgaagtga atcgttgaat gcacaataaa aaaatcccga
      121 ccctgagggt gtcgggatga aacttgctta agcaagaagc acttaaaaaa ttcgttacac
      181 caggaaatct gatgtgttca tcaccttatc tgcaattttt ttcgctgaca agaaaatatt
      241 cgcgctatga atgattaacg ctacttatga atagaaatgt gacgcgcgtc accccttggt
      301 ggctctggtg tcaacgggat cgctcccgaa aaaatcatct ttgattattt gtgctgtttt
      361 gagaatcatc ctggtaaggg gagtactgcg cgaccatgtt ttcaattcaa caaccactac
      421 tggtttttag cgatcttgat ggcaccctgc tggacagtca tagttatgac tggcaaccgg
      481 cagccccctg gctcagccgt ttacacgaag ccaatattcc cgtcattctc tgtagcagta
      541 aaactttagc ggaaatgctg tacttgcaaa aaatgttggg gctacaaggc ttaccgctga
      601 ttgcagagaa tggcgcagtg atccagcttg ctgaacaatg gcaggatata gacggttttc
      661 cacgcatcat ctcaggtatt agccatggcg aaatctgcca ggttttaaat acgctacgcg
      721 agaaagaaca ttttaaattc acgacttttg atgatgtcga cgatgcaacc attgccgaat
      781 ggacgggatt aagccgtagc taggcggcgc tgacgcagct gcatgaggcg tcggtaacgc
      841 taatctggcg cgacagtgac gagcgtatgg cacaatttat cgctcgtctg aacgaactgg
      901 gcttgcagtt tatgcagggt gcgcgcttct ggcacgtact ggatgcctct gccggaaaag
      961 atcaggctgc caactggatt atcgcgacct atcaacaatt gtcaggcaga cgcccaacca
     1021 cacttggcct gggcgatggg ccaaacgatg cgcctttact ggaggtaatg gattacgcgg
     1081 tgattgtgaa agggctaaac cgtgaagggg tacatctgca tgatgaggat ccggcccgcg
     1141 tctggcgaac gcagcgtgaa ggaccggaag gctggcgtga agggctggac cattttttct
     1201 ccgcccgtta agcgttatcg ctcgcgaata cccgattgcg cccggcctgt tttgcgaggt
     1261 aaagccgacg gtcggccaat gactggagtt gttcaaaatc ataatcaccg gtttcctcgc
     1321 tgctacttac ccccagcgag gcactgatgc gtatcgttgt actcttggcg atcagcatct
     1381 ctttttcatt taacttcagg cgaatacgtt ctgcgacctc cgcagcctgc gtcagattcg
     1441 cgcctggcag aatcacacaa aactcctcac caccgacccg cccggcaacg tcctgcgcac
     1501 gcaaggaact gctaattaat ccggcagcat gagaaagaac acggtcgccc gcctgatgac
     1561 caaagcggtc attaatcgct ttaaaatggt caagatcgac ctgaatgaca gaaaaaggat
     1621 gttggtgcgt ctgacacaat ttagcgagcg gacgggcttt ttcgaacagt gcgccacggt
     1681 tatataaacg cgttaaggtg tcgtgccacg cctgccactg caacgagctt tgcagaacat
     1741 acatgttgct gaccatccgg cgaatcacat accaggagag gagtaacatg gtggtaaaga
     1801 gcgcccacag cagggttaat gcaatgctga tactgccgaa atcgccgcgc acgccttcgc
     1861 ttagcgtatg gacgcgcgcc agcacaccgt cgaaatggtc cagacgttcc cagctaacat
     1921 agcgactgtc catacgaatg ccgccccgcg tgtcatgttc cattgcctgc gccagcaagg
     1981 ccagttcacg aggatcaaaa atattccctg ttggatggtc aggattggaa gaggtcaaaa
     2041 atctcagctt actgtcatag agctgatact caccatcgag gtttttatcg atggcgtttc
     2101 ttaaaaattg ctgcatagta cgcacgggaa tactcatccc cagcacgcca taccagtagt
     2161 tattactgtc taccggaaca ctgacggtaa cctgcggttc agtattgctg gcgtgttccg
     2221 gttgcgaagt gaaccagcgt accgcgcggt gacgattttc tcgttgcgaa tggccgataa
     2281 accagggttg ggtgacatag ccgtaataac gcgttggtac attgcgcgta aacaaggtcg
     2341 gctgcgtcga aacgtaaaat ccagcacgcg agacatacat cgcctgttca accatcgacg
     2401 aggagttgtg cgccagtcgc agcaagtaac caacttccag tgcagcggta atttcattgt
     2461 cgaggctttc attttcgcga gacaggaaat tcccctcgct gactaatgca tccgatacgc
     2521 cattgactgg cagggtgcgc cgtcggttga gttcgatttg ccaggcgtgc tcgtcgcgat
     2581 gctgttcgaa ctcggttacg gcattacgca gagaggtgaa atccagtggc gccacgagag
     2641 cttcgcgcat gccattacgc aaaaagatca gtttgtcgac gttatactgc aaatgcttat
     2701 cgagcgcgtt ggctacgttt tccagatgat tacgctagct ggagatatag gcatcttcca
     2761 gcaccaccac ttcacgccag gtgagcaagg tggaaaaaag caataccacg ataaagcaga
     2821 gattaacgac atgaccgggc cccaggcggc gtgcgagttt tttcaaccag ctctggtttt
     2881 ccatttttgt ctcgtgctgc accctgacga ctcccaacct gcttttttat gattctggca
     2941 taacccggca atagcgtacc gcaaaacagc gataatttta taatggtggc atgataagac
     3001 gcgactatca ttcattgcag gcattgggac accaggccgg atgtgatccg gcctggtgtt
     3061 aatgataccc tttcaaactt cttccgcaga acgactcccc tcccccggaa caagatcggt
     3121 ttcatgaaaa gcttcccgct tgaccccgta accgtcatac caacgacact caaccatacc
     3181 gctggagtat ccagtgacaa tcatccgcgg gccgccctct ttaaccgtaa cttcctcact
     3241 aaccataaag ctcatactcg cctccttttt tcgagtgaaa cttgttcacc ttagttgaag
     3301 atggcgaatt ttgcataata gccagtgcga gtattaatgt gcctgaccgc gcattttcgc
     3361 tactgctttc actcccagca ccacgatgcc gccgatgata aatccaagaa tcagatttaa
     3421 aacagtcggt aatatcatcg ccaccactgc actttgctgc ccggcgatat gttcaatagc
     3481 gtgatgcagc ggcgcaatac catgtaccac aatcccgccg ccgacgagga acatcgccag
     3541 cgtgccgaca atcgataatg ctttcatcag ccaaggcgcg ataatcaata accctttacc
     3601 taatgcctgc atcagcgcgc tagatttttc cgccagccaa taccctaggt catcaatctt
     3661 aacgataacc cctaccagac cgtagacgcc cacggtcact accagcgcga tgcctgaaag
     3721 caccagcacc tgattaagca acggcgcctc tgccacaatc cccagcgtga tggcgacgat
     3781 ttccgcagac aaaataaaat cggtacgaat cgccccttta attttgtcct tttcaaactt
     3841 cagcggatcc tgcgccgcca gcttctccag acgctgctgg ctctgcgctg gatcttcttt
     3901 atgtttacgc gcctccagca tatgcagcac tttctctacc ccttcaaagc agagaaacgc
     3961 gccaccaatc atcaacagtg gcgtaatcgc ccacgggata aacgcactga tgatcagcgc
     4021 cagcggcacc agaatcactt tattgatcag cgatcctttc gccacgcccc agaccacggg
     4081 aagttcccgg ttggcccgca cgcctgaaac ttgttgcgca ttgagcgata aatcatcccc
     4141 taatacaccg gcggttttct tcgccgccag tttgcccatc acagagatat cgtccagcag
     4201 tgtggcgata tcatcgagca acgtcagtaa actactcccg gccaaaagca ttccctctca
     4261 tttttattgt tgaagggtgt aagtatgaag caaaaacggc agctctgata cttgcttcac
     4321 aggtcaacat ttatttaaca atcaagtgca attatatctc tcgccagcag aatgattttc
     4381 acgtttacta tgaaccacct ttttatttcc gccgtgaggg attatgcgtt tccgccagtt
     4441 gttaccgctt tttggcgcgc tgtttgcgtt gtatatcatt tggggctcaa cctattttgt
     4501 cattcggatt ggcgtgggaa gctggcctcc gttaatgatg gcgggcgttc gattccaggc
     4561 agccggtatt ttattgctgg catttttgct actgcgcgga cacaaacttc ccccgctacg
     4621 tccgctgctc aatgccgcgc tgattggcct gttattgctg gctgtcggta atggcatggt
     4681 gacggttgcc gaacatcaaa atgttccttc cggcatcgcc gccgtagtga ttgcaaccgt
     4741 gcccctcttt accctgtgct tcagtcgcct gtttggcatt aaaacgcgca aactggaatg
     4801 ggtgggtatt gccattgggc ttgccggaat catcatgctc aatagcggtg gaaatttaag
     4861 cggcaatccg tggggcgcga ttctgatttt aatcggctcg attagctggg cgtttggctc
     4921 agtttatggc tcgcgcatta ccttacctgt agggatgatg gcgggtgcga ttgagatgct
     4981 ggcggcaggc gtggtgttaa tgatcgcatc gatgattgcg ggtgaaaaac tgacggcgct
     5041 cccttccctg tcaggcttcc ttgcggtcgg ctatctggcg ctgtttggtt cgattatcgc
     5101 cattaacgct tatatgtatt taatccgtaa tgtcagtccg gctctcgcca ccagctacgc
     5161 ctacgttaac ccggtggtcg cggtcttgct gggtacggga ctgggtggcg aaacgctgtc
     5221 gaagattgaa tggctggcgc tcggcgtaat tgttttcgcg gtggtactgg tcacgttggg
     5281 aaaatatctc ttcccggcca aacccgttgt cgcgccagtt attcaggatg catcaagcga
     5341 gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc ttcgccgcag atccactctt
     5401 ccagacgctc ggtaagcgcc gcatccgtca gcttctcgcg cctacgtaac gcacactccc
     5461 agacaatcag tacgcgccag ccgagctcct gcaagcgact gatatcgcgg cgatcgcgtt
     5521 caacattttt acctatcttc tccagccaga actcagttcg agtcgcaggc actttaaaca
     5581 gatagcagtg atgatgatgc cagaagcagc cgtgggtaaa tatcacgcag cgatattcat
     5641 caacgacaaa atccgggcgt ccgggcagac tggcgtcctg aacgcgaaac gcaaggccct
     5701 gcccggttaa caaactggcg aggcgcttct caatcgccgt atcacgcgtg gcaatcgcgc
     5761 gcatattttt gctgcgggtg gccttatcgt gaacgtcgac catgttgtgc ctcttgctga
     5821 cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc ggcaaagacc
     5881 ggcacgacca ccgagttgcc gaactggcga taggcctgag tgtccgaaac cggaatgcgg
     5941 aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg cgcattcacg aggcgttaac
     6001 cgacgtggac gatgttgctg attcagcgga tcgtcaaagt ctttctcacc cgtggccata
     6061 tcccaaccgc gatcgattaa aatttccgcg ccatctttgt aataacgagc agacagcgta
     6121 cgggtgacgc tttgcggatt gttcggataa accattccat aaccgaagcc gttaccgcgc
     6181 gcctgatgtt ttttcgcata tcgatagagg tacttccaca gcaccggcgt caggatatat
     6241 ttcgcctcaa ccatcgggtc cagcagctgc gccagcgtca ctcgctgtgc agggaaacat
     6301 tcgctgatat cacgcagagt aaaatcggct ttcagattca gatcgcgacg aaaacccacc
     6361 agcacgatgc gttcacggtg ctgcggcagg aaatgtttac catcgatgat tttcggatcg
     6421 tccggcccgt tatcttctgc atcggcaacg tcatagccca gttcgtccag cgtctgcatg
     6481 atgatgcgga acgtttttcc cttatcgtga ctcttcaggt ttttgacgtt ttcgagcaca
     6541 aacatcgccg gacgacgcgc gtcgataatg cgcaccacat caaagaacag cgtgccctgg
     6601 gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct ttttcgatac gccagccagc
     6661 gaaaatggct gacaagggaa accggccagt aaaacatcgt gttcaggaat gtgttgacga
     6721 atatgttccg ccgccgcctc atcactcacg ccttcttgat gactgagggt gatgtcgcgg
     6781 atatcttcat taaaatgatg cgtcgccggg tcgcaataat ggttggcttt ataagtgcgt
     6841 accgcatgtt tgttccattc gctggtaaac acgcactgtc caccaatcga ttcaaaacca
     6901 cgacggatgc cgccaattcc ggcaaataga tcgataaagc gaaacgcata atgcggatga
     6961 tgttccggtg gtttgggtaa caacgtttgc agatgggcaa actctttctc acttaaacgg
     7021 tgccatgccg agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca gtgattctca
     7081 cccacaccat taagctgcgc caccagcatt ttaacatcat aaatttgcag cagtttctcc
     7141 agcattgcct gtgcggcatt cccggtgctg tatgaatcgg ttactgatat attttcctgc
     7201 atagatttaa ccggccatct gagatggccg acaggttaac ataattactg ccgtttaggg
     7261 ccacgcgctc agccagcggg tgaaaacgca tctatcacct tgtgatcgat accttcattc
     7321 tcccccgcca gttcggcacc gagcttcgcc atcaaacagc gccaagctca atgcccctga
     7381 aacggcaaat acgcgcgcca ggccaatcgc ccccagggct tccagccgat ccgcatcctg
     7441 gacgatttta gcttccgttg ttaagggggc gatttgcgcg ctgaaactgt gagcggcaat
     7501 ggcatgacaa acggcctcaa ttttctccgc cggaaattgc acgaactctt cacggagcag
     7561 gcgacgcgtc tcttctgccg ccaggattga agaacgctgc cgttgcggat gatttttcgc
     7621 caggctcacg atatcgtgaa aataacaggc ggttaaaatc accagcatat cgacatcgtc
     7681 atctgccgcc agtttctgtg ccgtagccca gacacggcga aaatgacaca ggtcatgcgc
     7741 cgcgtcctga tgttgatgat ggtttttcaa ccagttctcg aactatgcct gccagtgttg
     7801 taactccata cgcgctccgt tgccaaagag tctcaacctt agcagttttt attcatagcg
     7861 tctgcaacgt gcggaacgag gtcgggtgta ccatgcaatg ccgcctaaca aggcaccaac
     7921 cagggccata aacagaaatc cgaccaatgc tcctaaccac ttccccgcgg tagatcctaa
     7981 atcactttca aatcccgaca cgggggcatc ggggatccgg ctcatcaccc agatgtatcg
     8041 cgccattgcc cagacgaaat agccacacca tgtatagaaa gccc
//
LOCUS       DAZOZX010000156         8057 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZOZX010000156 DAZOZX010000000
VERSION     DAZOZX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.156"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUPT_003544"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUPT_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUPT_003545"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUPT_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0820962.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFVSGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUPT_003546"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUPT_003546"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0820963.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUPT_003547"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUPT_003547"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0820964.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHUPT_003548"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHUPT_003548"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0820965.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHUPT_003549"
     tRNA            7783..7858
                     /locus_tag="ACHUPT_003549"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
BASE COUNT         1842 a         2080 c         2184 g         1951 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagaca
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtc
//
LOCUS       DAZOZX010000157         8048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZOZX010000157 DAZOZX010000000
VERSION     DAZOZX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.157"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..944
                     /locus_tag="ACHUPT_003550"
     CDS             <1..944
                     /locus_tag="ACHUPT_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0820966.1"
                     /translation="NQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVN
                     AAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGA
                     LDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQ
                     TDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLLPEGQ
                     NFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKL
                     PFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            1153..2118
                     /gene="lipA"
                     /locus_tag="ACHUPT_003551"
     CDS             1153..2118
                     /gene="lipA"
                     /locus_tag="ACHUPT_003551"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0820967.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2219..2422)
                     /gene="tatE"
                     /locus_tag="ACHUPT_003552"
     CDS             complement(2219..2422)
                     /gene="tatE"
                     /locus_tag="ACHUPT_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0820968.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2551..3339)
                     /locus_tag="ACHUPT_003553"
     CDS             complement(2551..3339)
                     /locus_tag="ACHUPT_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0820969.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            3432..3815
                     /gene="crcB"
                     /locus_tag="ACHUPT_003554"
     CDS             3432..3815
                     /gene="crcB"
                     /locus_tag="ACHUPT_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0820970.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(3869..4078)
                     /gene="cspE"
                     /locus_tag="ACHUPT_003555"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3869..4078)
                     /gene="cspE"
                     /locus_tag="ACHUPT_003555"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0820971.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4253..4813)
                     /gene="pagP"
                     /locus_tag="ACHUPT_003556"
     CDS             complement(4253..4813)
                     /gene="pagP"
                     /locus_tag="ACHUPT_003556"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0820972.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            5402..6787
                     /gene="dcuC"
                     /locus_tag="ACHUPT_003557"
     CDS             5402..6787
                     /gene="dcuC"
                     /locus_tag="ACHUPT_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0820973.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(6828..7169)
                     /locus_tag="ACHUPT_003558"
                     /pseudo
     CDS             complement(6828..7169)
                     /locus_tag="ACHUPT_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(7507..8016)
                     /locus_tag="ACHUPT_003559"
     CDS             complement(7507..8016)
                     /locus_tag="ACHUPT_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0820974.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
BASE COUNT         2066 a         1935 c         1808 g         2239 t
ORIGIN      
        1 ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga aggtaaacca caaatattta
       61 caaccttacg aaatattgat cttaaccttc tgactatttt tgaagctgta tatgtacata
      121 aagggatcgt taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta
      181 ttcagaaact gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca
      241 ctcctaccgc atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc
      301 ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca
      361 ctccctcggt cggagccctg gtccttcctg tcatctatcg ggcgattaaa actcactatc
      421 cgcagctttt actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc
      481 aaaccgatct catcatcgat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc
      541 tgttcaccga caatatggtg ttaatttgcc gcaagggaaa tccactactc tctttagaag
      601 atgacagaga gactatcgac aacgctgcgc atgtacttct gttaccggaa gggcaaaatt
      661 tcagcggtct gcggcagaga gttcaagaga tgtttccgga ccggcaaatc aatttcacca
      721 gctacaacat tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc
      781 ccagccgttt ttataacctg tttagtcgtt gctggccgct ggaaaaactg ccttttccgt
      841 ccttaaatga ggagcaaata gatttctcta tccactacaa caaattcatc ctgcgtgatc
      901 ccatcctgca cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag
      961 ccagatcaca gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg
     1021 accgctttgg ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa
     1081 tagttgataa ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg
     1141 aacacgcacg ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc
     1201 gataagatgg cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc
     1261 aagccggaat ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa
     1321 gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg
     1381 gcggaatgct tcaaccacgg cacagcaacg tttatgatcc tcggcgctat ttgtacccgc
     1441 cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag ctcctgatgc caatgaacca
     1501 gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt
     1561 gaccgtgatg acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt
     1621 cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgaatg
     1681 gatcgtgctc tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa
     1741 aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag
     1801 ctgctggaac gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg
     1861 ggtctgggtg aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt
     1921 gtgacgatgt taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa
     1981 cgttacgtta gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt
     2041 acccatgctg catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa
     2101 ggaatggaag ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct
     2161 ttttgaagcg ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
     2221 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
     2281 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
     2341 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
     2401 tggtaatact aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc
     2461 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
     2521 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
     2581 gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
     2641 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
     2701 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
     2761 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccacgaac
     2821 ccaggcggca ggaagtacca ggatttcagc tccctgtaaa gcctgtgcca gcgccagctc
     2881 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccttcca cctccagtaa
     2941 cggagctatt tcattaccag catcaacatg gcgtgattcc tgaatggcaa atgcatcata
     3001 gagatgcagt ttggcataac gggcgacgat gtttcctgcc tgaagtgcca ccagcatatt
     3061 ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga attgtcgtca tcatgttacg
     3121 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
     3181 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga
     3241 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
     3301 cacagatgta acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg
     3361 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
     3421 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
     3481 tagcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
     3541 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
     3601 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
     3661 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
     3721 gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
     3781 cattctggct gttttcggcc tcaaccgtac actaaagacc aaaaaaaacc cgctgattaa
     3841 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
     3901 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
     3961 cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
     4021 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
     4081 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttatcagt acaccaattg
     4141 tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat
     4201 gttgcttatt ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact
     4261 gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca
     4321 tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga
     4381 tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat
     4441 tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt
     4501 tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc
     4561 aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt
     4621 tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag
     4681 gctgttgcca ggtttgtgca atattttctc taaacgttgt catcctctca tctgcgttag
     4741 caaaaacttt accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt
     4801 tactcacgtt catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa
     4861 gctacaatta acataacctt aatagaccca acataaagaa taatctgaat agctgctcta
     4921 tttatttccg cttgttcaag ttaaatattt ccgcgcatcg attaaagatg atgcgtcgtt
     4981 ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt ttttgatgac aaaatgcaat
     5041 caaggaaaaa ttattttatt tttttaacga gatacaacaa tcatcttaac gaagtatata
     5101 atatatatcg tcaacccatg ctcatattag attatttttt gagcgcacag ccgcagctaa
     5161 caccattgca attaacaaat ttgcatcaat ccaccatcaa tttacacaca ttattatgtg
     5221 ataattgcca accgctaaat atgcgttttg ttatctatgt ataaaaacag caacttcaat
     5281 gtcttaatgg cagtttttct tgattttaat cagcattcat cgccaattta ttgggcatat
     5341 tttttcctta agctttagga agtttttatt atttactttg ggcctggaga caggaaaaat
     5401 tatgctgaca ttcattgaac tccttattgg ggttgtggtt attgtgggtg tggctcgcta
     5461 catcattaaa gggtattccg ccactggtgt gttatttgtc ggtggcctgt tattgctgat
     5521 tatcagtgcc attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag
     5581 cgccacggat atcgttgaat acgttaaaat attactaatg agccgcggcg gcgacctcgg
     5641 catgatgatt atgatgctgt gtggctttgc cgcttacatg acccatatcg gcgcgaatga
     5701 tatggtggtc aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat
     5761 gattgccgcc tattttgttg cttgtctgat gtcactggcc gtctcttccg caaccggtct
     5821 gggggttttg ctgatggcaa ccctgtttcc ggtgatggta aacgttggta tcagtcgtgg
     5881 cgcagcagct gccatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga
     5941 tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac
     6001 gctgcctatc tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg
     6061 ttatctggat aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac
     6121 cactgccccc gcgttttatg ccattttgcc gttcacgccg atcatcggtg tactgatttt
     6181 tgacggtaaa tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat
     6241 tgcctccatt ctggagttcc tccgcagctt taatacccag aaagttttct ctggtctgga
     6301 agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg gtgatgctgc tggttgccgc
     6361 tggggtattc gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc
     6421 tacctcgttt ggttcggcga gtatcatcct gatgctggta ttggtgattc tgaccatgct
     6481 ggcggcagtc acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc
     6541 gaaactggcg cactcttccg gcattaaccc ggcgtatttg actattccga tgctgcaggc
     6601 gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta gtcgttgcgg ttgccgggat
     6661 ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg gtgccggtgc ttgttgggct
     6721 agtgattgtt atcgttgcta cagagctgat ggtgccagga acggcagcag cggtcacagg
     6781 caagtaaata gtaatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg
     6841 tatatgcgtt gtggtctgcc aactttgccg tgaacaattt cagcaataat cagatggcgg
     6901 ctggcgcaat attcaagata acgcctggca gtggtgcggc tgatggtcag tgcctgcgcc
     6961 accgtttctg ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc
     7021 gtcaaggggt caatgccggt cggcagttcg tctttgggtt caccgcgagc ataggcatta
     7081 aacatctcat caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc
     7141 tggcggaaac gggttagcgt ttgccccagt gtattgattc acttgaagta cgaaaaaacc
     7201 gggagaacat tggattattc gggatctgat gggattagat ttggtggggc ttgcaagcct
     7261 gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag atagtaaaaa attgcttttg
     7321 tttcattgaa aaaacgaaaa acaaaaacac tataaatcat ttcaatcata gcttcaaaaa
     7381 acgttcaaaa ccgataacaa ccaggctgtc atcaaatgac tcatatcaca aatcagctca
     7441 tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga agtatgtttt agctggctca
     7501 tggttgttat acggcttttt tcgcatcttg tggttccaac aaaggagcta caggctcttc
     7561 ctgtttatga acaccataca ttgacaaaat ctcttggcga gtttctggag gtaatttatg
     7621 cagcgccaaa gctgtttgta gtgtttggcg atcaataccc agcgttgcca atagggtgtc
     7681 aacggagatg gaggggcgtt cttgcattaa tccggaaagc ccatatttag ccatgtttag
     7741 ctctatctct cggacttcat tttctcctag tttattgatt aacagggaga acattttcaa
     7801 tgtatcagca ttcatttctt cgatagcaat tttttgcata gccggtattg ccgggatata
     7861 ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc ggccatcctt ctttctttgc
     7921 tcgtttatgc accccctgta ttgtcccagg cattccatcc agttcagtaa tttgtttggg
     7981 tgtcatccac tggtttatct catttgcgct tttcataaac taagtttacc agatttatct
     8041 atataaaa
//
LOCUS       DAZOZX010000158         8045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZOZX010000158 DAZOZX010000000
VERSION     DAZOZX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.158"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(81..272)
                     /locus_tag="ACHUPT_003560"
                     /pseudo
     CDS             complement(81..272)
                     /locus_tag="ACHUPT_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            270..594
                     /locus_tag="ACHUPT_003561"
                     /pseudo
     CDS             270..594
                     /locus_tag="ACHUPT_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(624..3536)
                     /locus_tag="ACHUPT_003562"
     CDS             complement(624..3536)
                     /locus_tag="ACHUPT_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR0820975.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4019..4861
                     /locus_tag="ACHUPT_003563"
     CDS             4019..4861
                     /locus_tag="ACHUPT_003563"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0820976.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4864..5952
                     /gene="capU"
                     /locus_tag="ACHUPT_003564"
     CDS             4864..5952
                     /gene="capU"
                     /locus_tag="ACHUPT_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR0820977.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5957..6907
                     /gene="virK"
                     /locus_tag="ACHUPT_003565"
     CDS             5957..6907
                     /gene="virK"
                     /locus_tag="ACHUPT_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0820978.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6972..7916
                     /gene="lpxM"
                     /locus_tag="ACHUPT_003566"
                     /gene_synonym="msbB"
     CDS             6972..7916
                     /gene="lpxM"
                     /locus_tag="ACHUPT_003566"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0820979.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2167 a         1801 c         1916 g         2161 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta aggccgccgg ggacgttttt ttaccaccgg gagtttgggc
      121 ataacggtgc tttcagtaag tacgacaggt actgtggagg cacttccgta aagaagggca
      181 tcaagctcct gctccgacat acctgcaggc aaaggccatg aaagccctga tttccggaag
      241 cgagcgaaca gtccacaaac tgttgaacgt ggtgtcagcg gcggacaggg ataagccctc
      301 gtcagcagtt ccgtcagcac tgcgacagcg ttctttaccc ggtcaaaaca gcgttgcggt
      361 gcaccacgcc tgacggatga actgcgtgct cagggttacc actttaacgt aaaaaccgtg
      421 gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt cagcccggtc
      481 agttaccgcg cacatggcct gcccgtgtca gaaaatctgc tggagcagga tttttacgcc
      541 agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga tgaagttcgg
      601 ttacatcctg tcagcacgga acctcacgct ttttgagcgg cacgacggat cccccccacc
      661 cagtatccga cagacttttc actgcgttcg taaaaaccct tcttccctcc cagcatatcc
      721 actaattccg gtgacatatc ctgcagataa gtctgcaaat catgccccag atcttccggt
      781 gactgcaact cataaaccgc gcgccacaca tcacccttcc ttattttaag ccccgggtta
      841 agaatatact gtccgtggtt cagcctcaga aaaagctttt ttccataagg atttttgctg
      901 ttaacttcat gttgagagag ctggctgctg atataactcc ttcgtttgcg gcgttccggc
      961 agcaacgctt caggaagatg aaccacacac tctgccagtc gtgccgcact gaaggcttcc
     1021 gaaaaaaaca gagcacttcc cagatgcgtg tggaacagcg cctgcatcag gatcaccaga
     1081 aacattgcca tctgccggtt gtccagtttg atgaggcgtc cttccacctg cagtgacaga
     1141 ctttcaggtt tcagcagggt atacaactgc gcacttttct gttgcgcata acggggcgta
     1201 ctgaccgccg ctgacagaac ttgatgataa gccgccagcc cgtcattacc ggtcagcaag
     1261 ggatctgcac cacgctccag cagtaactga accagtgcaa tattacctgc ccgggctgct
     1321 gacatcagcg gagtaagatt aaacggcgaa cggtgatcaa caccataacg ttcaatatcc
     1381 cgcaacacgg caaccgggtt attcattgag tatggccgga aatacttcag ctccagtaac
     1441 tgcagagtct tcgttctggg ctgccttgtg ggcttaaaat cttcccggta cagtgcatta
     1501 tacagcggga acagggaata aatcagactg tactccagcg cctgcagttg tatggtttta
     1561 tcggctgtac ctgcgaggat atgctgtttc cactgtcgtg catcctctgc ggtgataatc
     1621 tgccatggca tctcagatgt ctgcaggatc cggctacgga tggtgtccgc ctgctcctgc
     1681 ttcccctgtt tttcaagcag atgtgcggtt ttctgccact catcacggct ggaaatctct
     1741 tcctgcagat tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca cagaggatgc
     1801 tccacctgct gttcaacaag atacacattg tgtttagcac gggtgagcgc aacatacagc
     1861 gcgttggtaa aaaatttata gatttccgct gaacgatcct gcctgtcccg cggacgggaa
     1921 taacgggcct cgccctcaag atcagccggt gtaagaccct cgcaaatatc atcaaatgcc
     1981 tgccgggcag cgcttactat attgtaaagg atcaccgttt cgtattccag ccccttaacc
     2041 tcctgaaccg aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc tttctgttcc
     2101 ggatgcatca caatgaccgc tacacgattc gaaagtgagg ttttggcatt cagttcctgc
     2161 tttgtctctt ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc gcagcttctg
     2221 acaaaatggt gactttcccg atcaatagca ctaaagcgga cctgtttgag tctcagcaac
     2281 cggttagcca gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag gatattaacg
     2341 gttgtgtcgt ttccctgttg ctgccggaaa aagagacttt tgagcgaact ccatgagaaa
     2401 aagctcggat gaactatctg attggcatca ccacagagta aaaactgacc gggatgacgt
     2461 agcgttttca gtaccagttg cagttgaaca ggcgtcatat cctgaacttc atcaacaaaa
     2521 atgacatcat aacggggagt tgccaacgaa agatactgat gactgagcag attactgtca
     2581 tactgatgcg actgtttcag ccatgcgata taacgttcaa acaggacata gaccgtcggg
     2641 cggtcctcca tgccatagag cgactgccgt atccccagtg acagatacgc ctcccgggac
     2701 agcggcccat tcccggaggc aaccgcaccg atcacgcccc tgaactcctc atacagggta
     2761 tgggcagccc ccagtgcggc gatagccctg ttgcggggca gccagtcgct gaacgcactg
     2821 aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt cagcgaaaga
     2881 aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt acgcgctttt
     2941 tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg cttcattttt
     3001 tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag cggtagcggg
     3061 tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag gcggtggaaa
     3121 cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac aggatccggc
     3181 actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag aaaacgtgaa
     3241 gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc accgtggtag
     3301 cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta aagattggtg
     3361 tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg tgcaaatttt
     3421 ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt catggctgcc
     3481 ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact gaacaacctt
     3541 gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc agttatggaa
     3601 taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc agtagctgca
     3661 ggcctgtcat gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg tcaccccgta
     3721 aataaactct gcgctgaaat tccttttttt cattggagta cctgtgttgt tctgaggtga
     3781 acatatcacc tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt cttagtgagt
     3841 cgtttttaaa agatatgata atatatcatt gtttatgaat tgttgatgtt taatttagta
     3901 aattatatca aatatgccat gggtttgcta tgttattcat ccagtatctg gaaataaaat
     3961 gcaccagctt tattgggttt tccttttgtc tctgcttgct aggtgccaga aactttaaat
     4021 gttaaacgaa gggggcattt tgttcaaagc caatcatgtt ccggtattaa tgtatcatca
     4081 tgtcagtcat tgccccggac tggttacact ttctcccgtg acattccgga agcaaataaa
     4141 atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt ttttttatcg
     4201 gggcggaaaa ctcccccgga aaagcgttat gttgacattt gatgatggat accttgacaa
     4261 ctggtttcag gtatatcctt tattaaaaga gtttaatctc aaagcacata tttttttaat
     4321 taccggtttt attgggaatg gtccggttcg tcactctcct ggaaaggaat attctcatcg
     4381 cgattgcgaa catcaaatag cgacagggaa tgctgataat gttatgcttc gctggtctga
     4441 agtaaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacgc atactcatac
     4501 acgctgggat aaaaagttca gttcacgaga agaacagtgt aaacatcttc ggcaggactt
     4561 gttgagcggg agggaatatt tgaaagaaat gacaggaaaa tgcagtaaac atttgtgttg
     4621 gccggaaggt tattataaca aggattatat tcaggtcgct gaagaacttg gattctatta
     4681 tttatataca acagaaagaa gaatgaatgc tcctgctaaa ggaactacac ggataggaag
     4741 aataagcacc aaagaacggg aaagttgcgc atggctgaaa agacgacttt tttattatac
     4801 aaccccgttt ttttcatcgt tgctggcctt tcataagggg ccaagattac cggatgatta
     4861 ataatgaata tactatttac ggaatcatca ccgaatatag gtggtcagga attacaggct
     4921 gttgctcaaa tgaaggccct gaagaaaatg gggcattcag ttctgcttgt ctgcagggaa
     4981 aacagcaaaa ttgcttttga agccagtaaa ttggggattg atatcacatt cgcgttattt
     5041 cgaaacagtc ttcacatccc tactgcatgg agattactcg gaatagttca tggttttcag
     5101 cccaatgcaa tcgtttgtca cagtgggcat gatagcaata ttgttggttt agtacggtta
     5161 ttcactagga aacatccatt cagaattatc aggcaaaaga catatttgac acgaaaaaca
     5221 aaagtttttt caataaatca tttttgcgat gaggtgattg ttcccggaac aagtatgaag
     5281 acacatctgg agcaggaagg atgtcgaacc cgggttactg ttgtgcctcc aggctttgat
     5341 ttccagaaat tatacgttga ttcccgaaac agtttgcctc caaatgttct ttcttggctg
     5401 gcgtcccgaa ggggatgccc cgttattgct caggtaggaa tgttgcgccc ggaaaaaggg
     5461 cacgaattta tgttgaattt actgttccat ttaaaaatga atggacgaca gttctgttgg
     5521 ttgattgtgg ggtctggttc gcctgaactg cgggagcatt tacagtatca gattgacagt
     5581 atgggcatgc atgatgatgt ttttattgct gacaatgttt ttcctgccgc ccccgtatat
     5641 cgggttgcca gtctggtggt tctgccttca gaaaacgaat cgtttggtat ggtactggca
     5701 gaagcatcgg cattttctgt gcctgtactg gccagtcaga ttggtggaat ccctgatgtt
     5761 attcagaata accagaccgg gacattgtta ccagcaggta ataagcacgc atggatgtgc
     5821 gccctgaatg atttttttaa tgaccctggg cgtttttatc agatggctcg ccaggcaaaa
     5881 caggatatag aagagcggtt tgatattaat aaaactgcgt taaaaatact cacattagcg
     5941 aagcacaagt aacaatatgt tttctgtaag taacttatca tttatcggtt tccttaaaag
     6001 gattgttttt tcctcagatt cactcccggg gaagtgggaa cacagaaaat tccggttcat
     6061 gtacattttg cgatgtgcta taaatccggt tgccagtatt cgatattatt acgaactgcg
     6121 ttccttgcag tgcattgagg atattctggc aatacaaccc acgttgccag caagaattca
     6181 tcgaccttat ttgcataaag gggggcgagc ctggtcccgg gggcaatata ttcttgagca
     6241 ttatcgcttt gtgcaaaatt tgccagaaaa atattctgaa tttttgtttc cacaaaaatc
     6301 ggtatcatta gtccaattca tcgggaaaga tggagaggat tttgatatcc agtgttctcc
     6361 cagtggtttt gaccgggaag gagagttgat gttgtcattg tttttcaata aaatagtgat
     6421 tgcccggtta acattttcag tcattctgac tcaaaatgga cacaccgctt ttattggcgg
     6481 attacaggga gcccccaaaa ataccggacc tgatattatt cggtgcgcaa cccgggcctg
     6541 ttatgggctt ttcccaaaac ggattatttt tgaagcattt tgtgcgttga tgaaagcttg
     6601 taatgtctct gagtgtctgg cagtcagtga gcacagtcat gttttccggc aattgagata
     6661 ctggtatcag aaacgcaaaa cctttgttgc tgtctacagt gatttctggg agtctgtagc
     6721 agggaaaacc tgtggtgact ggtacaaatt accaacacag gtggttcgaa agccgctaag
     6781 caatatagct agtaaaaaac gctctgagta tcgaaaacga tacgctttgc tggattatat
     6841 ccatgaaact gctatccgct ctttggatgc atatcctgta aactcagaac atcaggactt
     6901 aaattaaggt ttgatgtatt ctctgaataa aatattaatg atgattttgg taggggcatt
     6961 cgcactaaat aatgaaaaaa tacaaatccg agtttattcc tgaatttaag aaaaattatc
     7021 tttcccctgt ttactggttt acatggttcg ttttgggaat gattgcaggt atttcaatgt
     7081 ttcccccttc attcagagat cctgtcttgg ccaaaatagg gcgttgggtg ggcagattga
     7141 gcagaaaagc tcgtcgcagg gcgacgatta atttatcgct ttgtttcccg gaaaagagtg
     7201 atacagaacg ggaaataatt gtcgacaata tgtttgccac agcattgcaa tccatagtga
     7261 tgatggcaga actggcaatt cgtggtccgg aaaagttcca gaaacgagtg ttctggaaag
     7321 gcctcgaaat tcttgaggaa attcggcaca ataacagaaa tgtgattttt ctggttcccc
     7381 atggctggag cgtggatatt cctgcaatgt tgctggcagc tcagggggaa aaaatggccg
     7441 ccatgtttca tcagcaacga aatccagtga ttgattatgt ctggaattct gtacggcgta
     7501 aattcggggg gcgcttacat tcccgggagg atgggataaa accatttatt cagtcagtac
     7561 gccagggata ctgggggtat taccttccag atcaggatca tggtcctgaa tacagtgaat
     7621 ttgctgattt ttttgcgacc tataaagcga cattaccaat tattggacgt ctgatgaaca
     7681 tcagtcaggc tatgattata ccgcttttcc cggtttatga tgaaaaaaaa catttcctga
     7741 ctattgaagt tcggccacca atggatgcat gcattgccag cgcggacaat aaaatgattg
     7801 cccgacaaat gaacaaaaca gtggaaattt tggtggggtc acatccggaa cagtatatct
     7861 gggttttaaa attgttaaaa acgcgcaaat caaacgaagc ggacccgtac ccttgaaatt
     7921 ataaagtaca ggtatttcca ctagttgttt cttacaggtt accaatcgaa acacatcccc
     7981 cttccgtgtg ttgcccgttt ggcacggagg actgtgtaga tgcagttcgt taaatttaat
     8041 ttatg
//
LOCUS       DAZOZX010000159         7935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZOZX010000159 DAZOZX010000000
VERSION     DAZOZX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.159"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(282..357)
                     /locus_tag="ACHUPT_003567"
     tRNA            complement(282..357)
                     /locus_tag="ACHUPT_003567"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Asn,seq:gtt)
     gene            complement(458..1929)
                     /gene="yeeO"
                     /locus_tag="ACHUPT_003568"
                     /pseudo
     CDS             complement(458..1929)
                     /gene="yeeO"
                     /locus_tag="ACHUPT_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2090..2165
                     /locus_tag="ACHUPT_003569"
     tRNA            2090..2165
                     /locus_tag="ACHUPT_003569"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2123..2125,aa:Asn,seq:gtt)
     gene            complement(2203..3153)
                     /gene="cbl"
                     /locus_tag="ACHUPT_003570"
     CDS             complement(2203..3153)
                     /gene="cbl"
                     /locus_tag="ACHUPT_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0820980.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3255..4172)
                     /gene="nac"
                     /locus_tag="ACHUPT_003571"
     CDS             complement(3255..4172)
                     /gene="nac"
                     /locus_tag="ACHUPT_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0820981.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4500..4575
                     /locus_tag="ACHUPT_003572"
     tRNA            4500..4575
                     /locus_tag="ACHUPT_003572"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4533..4535,aa:Asn,seq:gtt)
     gene            complement(4631..5566)
                     /gene="ldtA"
                     /locus_tag="ACHUPT_003573"
     CDS             complement(4631..5566)
                     /gene="ldtA"
                     /locus_tag="ACHUPT_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0820982.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5628..6707)
                     /gene="cobT"
                     /locus_tag="ACHUPT_003574"
     CDS             complement(5628..6707)
                     /gene="cobT"
                     /locus_tag="ACHUPT_003574"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0820983.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6719..7462)
                     /gene="cobS"
                     /locus_tag="ACHUPT_003575"
     CDS             complement(6719..7462)
                     /gene="cobS"
                     /locus_tag="ACHUPT_003575"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0820984.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7459..>7935)
                     /gene="cobU"
                     /locus_tag="ACHUPT_003576"
     CDS             complement(7459..>7935)
                     /gene="cobU"
                     /locus_tag="ACHUPT_003576"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR0820985.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2195 a         2127 c         1717 g         1896 t
ORIGIN      
        1 attacgtata gtttcccgat aaagacgcga cagctacgtc ccgcataagg catttttctc
       61 tttatctttg tacggtactt catggaacag aatttttgac cttgtgaatc gtgatatctg
      121 ttggggaggg gcaatttgct cactgaagcg tgagactcga ttaagcgcac gaaacacaga
      181 aatcaaaaaa cccggccact tttttacaaa gtggccgggt aaaaataatt tttatttttt
      241 aactgtcctg agtctcatag agatgtctca aaactaaaat ttggctcctc tgactggact
      301 cgaaccagtg acatacggat taacagtccg ccgttctacc gactgaacta cagaggaatc
      361 gtgagaacga ggcgaatatt agcgatgccc acccacaatg tcaaagcctg ttttttaaat
      421 ttgaaatcgt ttgctgaaat aatctgcatt ttgtcgttta ttccgacaca actggctttt
      481 tttcacactt ttgcggctcg gatcgagggt atttccacag ccaacgtcca gtaaccattc
      541 gccagtaaaa cagcacggcc cgcacagccc agtcggcaaa cattcccatc cagacaccaa
      601 ccacacccca gccaagcatg attccctgca cataaccgac tacaacccgg caaccccaca
      661 tgctcaacat agaaacccac atggcgtaac gggcatcacg agcaccctta aatccagcgg
      721 gtagcaccca tgaggcggac caaataggca taaataaagc atttagctaa atcagaatca
      781 caacgacgtg tttaacctgt ggatcctggg tgtaaaacga tgccataacc ccggcaaagg
      841 gagccgttag ccaggcgatg gccgttaatc caagagtgga cagccagaac acatgccgca
      901 actgaatctc tgcttgcgct atctgcccta cccccaacct tcggcctgta atgatcgtag
      961 aagcagaacc gagcgcactt ccgggtaagt tgataagagc cgcaattgag aacgcgataa
     1021 aatttccggc aataacgctg gtccccatcc cggcaacgaa catttgggtt aataaccgac
     1081 cactggtaaa taacactgat tcgacactcg ctggaatacc aatccccatg acttcccaga
     1141 taatgctaaa attcagcggt ttaaaatagc tctttaacga aatccttagc gcaggattaa
     1201 aaccaatcgc cagcacccac aaaattgcaa ctgcgccaat ataacgagaa atggttaaac
     1261 ccaacccagc cccgaccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt
     1321 aagaatattc aggctaccgt taatcagtag cggtattttc gtattccctg caccacgaag
     1381 tgccccgcta ccaataagag tgatggcagc ggctggataa ctgagtacgg tcagctccag
     1441 ataagtcaac gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat
     1501 ttgttcgcca aaatgatgaa ttagcgttgc caacagtacg gcaaacaacg tcatgatcac
     1561 caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc
     1621 tacaacgaca gtagtaccaa gatcgatagc tgcaaaaaaa gccataatga ccatattgaa
     1681 gctgtccgcc aaccccacgc cggccatcgc atcttttccc agccaactga ccagaaaagt
     1741 gctcaggacc cccatcaaca ggacacaggc gttctccatg aagataggaa cagcaagcgg
     1801 ggttatctcg cgctagaaca acactttgta gctcttgcgt ttagagtgcc agcgagtgcc
     1861 gtgaacaacc tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg
     1921 aaaccacatt tcaaatgatg agggagaatc agcaaagctg caaagatttt cgccaacaaa
     1981 ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa cagttgggaa atttaaaaat
     2041 cgggtttgac aaaatatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt
     2101 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     2161 agccaaattt ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat
     2221 ttcctcttca ctgttttcca tcacctggcg cttgatatcc tcaactgaca gtcctgcatt
     2281 acaaagttcc agaaagcgcc aaacatagtt acgctgaagt tgtcctcgct tcagtcccaa
     2341 ccagacagta ttagcatcga aaagatgccg cgtatccagg cggattaaat tctcttcctc
     2401 ttgttcgccg ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt
     2461 tttaatgaca tcagaatcct gtgcacttaa tacaatatct gccggcaaac ctttgcgggt
     2521 aaatgcgtca tcagtgcgtg agcgccccgt aattccctgt cggtaagtaa ttaacggcca
     2581 cttcgctatt gattccagcg tcaatggtgt aatttgcgtt aagggatgat cgagtggaac
     2641 aagcaaacta tggtgccaac aaaaccatgg gaaggcgacg agctgcgggt cattactcaa
     2701 acgctcgctg gcgataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg
     2761 tggcgaaccc tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat
     2821 gacctctggc aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacaccaga
     2881 cgtatcgttg gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg
     2941 ttctgcaatg accagtaatg ctttgcccgg ttcagtcatc cccagcagtc gcttacctcg
     3001 tcgaacaaat atttcgatgc caaattcatc ctccagttcc cgaatatgac ggctaacgcc
     3061 tgactgtgag gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc
     3121 ctcgcggatt atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatatcc
     3181 cgttattgtt aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca
     3241 tcccgatata acgcttagct caccaattgc cactgccttt tttccatcac tggagaacta
     3301 atcactgaca ttaacaactc tttcactgcc tgtgcctgtg gtgacaagtt tgctctggcg
     3361 ggtaaattta aggacaaaga gagactcatt gaaggagtgg taatgcgtga catccaccca
     3421 tttactgcgc cacataacga acgcgcagcc gattcaggta atactgcaac gcccatgccg
     3481 ctggcaatcg ctgcggtaag cgtggcaata gactcaattt caccaatgac ttttgccgtg
     3541 agccgccgta gggaaaaagc ctcatcaaca cgaagtctaa cagcgctgta atcactgggg
     3601 agaaaaaggt tcatttgcgc aatagcatta acatcaacgt tttgccccgg gcaatcttga
     3661 gttcctacca ggaaaagatc ttctttcagc aaagcctgac tggatacccc agccacaggg
     3721 gaatgctcat aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact
     3781 gcaccactat tttcatgaag atagataacg acctccggga attcagcgcg aaccgcctgt
     3841 aataagggca tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc
     3901 cccgataatg actgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa
     3961 atggcccgtg catgggtata gagaattttt ccggcgtctg ttggcgtaac gccccgtttt
     4021 gtgcgaatca aaagctgttg atttaactca ccttccagta tggcaacctg ttggctgagc
     4081 gctggttgtg cgatatgcaa tacttcagca gcctgggtca ggctaccaat atctacaatt
     4141 tttacgaagt atttcaggcg tctgaagttc atgttgcctc cggtttttaa gaatcggccc
     4201 aagtgccgcc attacttaca accagtatgc aagatgcctg ccagttctat tttggcgttg
     4261 atgcgcaagc taaccaactg tcaaataaga gattatgata gattcgtcat tggccctttt
     4321 tatcagcagt cgcattcacc tgccctataa aaggagggta tgcaccacga tggttcatta
     4381 cccaataaga ttgaaagctc accactttgt tgaaattggc agcaaacaaa caaaaaaaat
     4441 gcatttcacc ctttgacatc accatgcact gccattaata tgcgccccgt tcacacgatt
     4501 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     4561 cagtcagagg agccaaattc aaaaaagcct gctttcgagc aggcttttta ctttctaatt
     4621 accaacgctc ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc
     4681 gatgttgcaa cgcagcattt gcgcgattta catcaacttc ttgcccgttg ataaacgccc
     4741 gcaaagatgg ggttaccggc aatggcactt ttcggtcaga ctcatattct gcacgattgc
     4801 gcgacagcgg ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca
     4861 ctggctgatc aataatttgt acacgagtgc ctacaggaac attatcaaac agatatttga
     4921 tatcgtcatt acgcagacga atacagccct gacttacacg gagcccaata ccaaaattgg
     4981 cattggtacc gtggatggca tacaacctgc caatataaat cgcgtacagc cccatggggt
     5041 tatcgggccc cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc
     5101 ggatgttcgg cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc
     5161 agttacgcgg ggtttctcgc ccagcctggc cgataccaat aggaaagact tccactgtat
     5221 tactgtctgg cggatagtaa taaagacgca tttcagcgac gttaaccaca atccctttac
     5281 gaacagtggc gggcaaaatc agttgctgcg gaatggtgag ttgtgagcca ggcttcggca
     5341 aaaaaacatc agcgcccgga ttcgcttcca gcatattact taacccttgc ccgtattgtg
     5401 cggcaaaagt ctccagcggc tgagtattgt gatcaggaac agttacagta agcgactgcc
     5461 ccactaaacg gctaccctct ggaggtaatg gataagttac cgccaggcta ttttggctgg
     5521 caaaaagcag agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag
     5581 tcgcgaatac tatccgataa gtatagcttt tatcagactt ttcgttttta gctgttcaaa
     5641 tcagaagtcg tattccccgg tagaacaata ttactggcag caagttcgcc catgttgttg
     5701 tatatcgcac aagcagcttc gatgatggac atcgccagag ctgcaccact cccctcacct
     5761 aaacgcatat ccatattgag ataaggctcc agccccaaat gcgagagcgc tatacgcgcg
     5821 cctttttctg ccgacaagtg agaaggaatg agatacggtt tgattgcagg agacatctgg
     5881 caggctgcga gcgcagcagc ataagaaaga aatccatcca gcaacacggg taaaccgcag
     5941 gaagcagcac ctaacatcac tccagctatt ccgaccaaat caaatccacc cacttttgcc
     6001 aggacatcaa caccatcctg aggatttggt tgattcaacg taatcgcccg acgcacaaca
     6061 tcaattttat tagccagttt atctgtcggc aggtttgcgc caatcccaac cacttcttca
     6121 ggatcccggc cagtgattgt gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc
     6181 agttcaccta caccaaacag cgtgacaccg tttttcgcca gctcccgcgt ataacatatg
     6241 acgtccaaaa gcaacttttc agcctgacgg cgactcattg ccggagctga agcaatattg
     6301 ccgctacctc gtgcgacacg catgttgata agcccgggga taggctcagc agtatcaata
     6361 ccaacatcaa ttacgtggac gttagcgccc gcttgtgctg ccagcacaca cacgccggtt
     6421 gttccacggg tcatattttc agcctgtatg gctgtcactt cttttggaga aatagcgacc
     6481 ccttcctccc agacgccgtg atcggcacac ataaccagta ccgctttttt gcccacatgc
     6541 ggtatgccat tcacccccgg cattcctgcc agttgtatgg caagcgcctc cagctttcct
     6601 aggctaccaa caggtttgaa taacccgtca acatgccgtt gtgcacgcga catagcggca
     6661 gaattgatgg ctgggatcgt attcagtaaa tcggcaatta tttgcatgtc acgtttcttt
     6721 atagcagtgc cagcaggaag accagttcac caagctctat ggctgctccc agcgtatcgc
     6781 ccgtttgccc gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg
     6841 tcaccaccat agcagccaca ccatgcatac ccagcaataa tacggcagca aaaatagcgg
     6901 ctaagccgag tgtgacgcag gtttgtcgcc catcaatttt gccaatgaac acattgccaa
     6961 gcccttcttc ccgcgcataa cgatgacgat acatcaataa tgcggcagtc ccgcggctga
     7021 ccgcacatgc tgccgccagc gatgcaagga tcggttcgcc acgcagagcc aactcgctta
     7081 acaccagaat ctttgccagt accacaaaaa tcaatgccag accgccgtgg gtgcctaaac
     7141 ggctatcacg catgatttcc aacatgcgat cgcgactacg tgcagaaaat acgccatcac
     7201 aggtatcagc gaggccgtcc agatggaatt ccccggtcat cagcgccagc acaagtacgc
     7261 taaacagtgc cgccagtggt gcgccacacc atgcctgcag caccatgaag accagcccgc
     7321 taatcgcgcc aagcaataat ccaatcaaag gaaaagtaat aataccgcga gaataatgtt
     7381 cgaaatccag tccctgggac cagcgacgcg gtacaggcag acgcgtaata aatgagagca
     7441 tcgcccaaaa taatttgctc atttgatttt tactccaata cccgaaacca ccagccatac
     7501 ttcatttgcc gcagcggcca actgctgatt tacccgcccg gcaatatcac gaaaatgtcg
     7561 tgccagacga ctctccggca caatccccat tcccacttcg ttagtcacta atacaacctt
     7621 tgcggggcaa cgttggcagg cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc
     7681 catcgcctga taatcccatt catcagggtc tttatcgccg ccataatcaa acaacagatt
     7741 agtcaccatt gtggtaacgc attccagcaa cacagcctca ttcgggttaa tgtctgcatg
     7801 aattaattca tcaagatgtt gccagcgctc cactgtgcgc cagtgctccg ggcggctttg
     7861 ccgatgatgt tctatccgtg cagccatctc atcatcaagg atttgcgagg tagcgatata
     7921 cagaacctgt gaaga
//
LOCUS       DAZOZX010000160         7746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZOZX010000160 DAZOZX010000000
VERSION     DAZOZX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.160"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHUPT_003577"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHUPT_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR0820986.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="ACHUPT_003578"
     CDS             1104..2564
                     /locus_tag="ACHUPT_003578"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0820987.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACHUPT_003579"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACHUPT_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0820988.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACHUPT_003580"
                     /gene_synonym="ydfH"
     CDS             complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACHUPT_003580"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0820989.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACHUPT_003581"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACHUPT_003581"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0820990.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="ACHUPT_003582"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="ACHUPT_003582"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0820991.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="ACHUPT_003583"
     CDS             complement(6729..7247)
                     /locus_tag="ACHUPT_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0820992.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7746
                     /gene="ydeI"
                     /locus_tag="ACHUPT_003584"
     CDS             7523..>7746
                     /gene="ydeI"
                     /locus_tag="ACHUPT_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR0820993.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1941 a         1813 c         1957 g         2035 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaagg
//
LOCUS       DAZOZX010000161         7537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZOZX010000161 DAZOZX010000000
VERSION     DAZOZX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.161"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUPT_003585"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUPT_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUPT_003586"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUPT_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0820994.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUPT_003587"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUPT_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUPT_003588"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUPT_003588"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0820995.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUPT_003589"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUPT_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0820996.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUPT_003590"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUPT_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0820997.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="ACHUPT_003591"
     CDS             complement(6512..7204)
                     /locus_tag="ACHUPT_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0820998.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1826 c         1986 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac cccccgcgcc gctgagaaaa agcgaag
//
LOCUS       DAZOZX010000162         7519 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZOZX010000162 DAZOZX010000000
VERSION     DAZOZX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.162"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(283..1683)
                     /gene="asnS"
                     /locus_tag="ACHUPT_003592"
     CDS             complement(283..1683)
                     /gene="asnS"
                     /locus_tag="ACHUPT_003592"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0820999.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1852..3054)
                     /gene="pncB"
                     /locus_tag="ACHUPT_003593"
     CDS             complement(1852..3054)
                     /gene="pncB"
                     /locus_tag="ACHUPT_003593"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0821000.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3320..5935
                     /gene="pepN"
                     /locus_tag="ACHUPT_003594"
     CDS             3320..5935
                     /gene="pepN"
                     /locus_tag="ACHUPT_003594"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0821001.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6142..6909)
                     /gene="ssuB"
                     /locus_tag="ACHUPT_003595"
     CDS             complement(6142..6909)
                     /gene="ssuB"
                     /locus_tag="ACHUPT_003595"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0821002.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6906..>7519)
                     /gene="ssuC"
                     /locus_tag="ACHUPT_003596"
     CDS             complement(6906..>7519)
                     /gene="ssuC"
                     /locus_tag="ACHUPT_003596"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0821003.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1909 a         1998 c         1906 g         1706 t
ORIGIN      
        1 aattttgttt ctgatggaaa ctttatcgac ctggcacaaa atcctctttc agacatccag
       61 aatgccaaaa aatagtatga aaattgtgct atcagctaag aaaaaactaa tccgcaataa
      121 gagtacgggt tattttttgt gcgtaaatgt tgcaatgtaa tattccgtaa caggatgatc
      181 gttatggcgg gggtgcaaat agattgcttg acttaacaaa cagataactt gacagaaagg
      241 ataaaaaaga gccagcaggc gctggctctt tgaggaagaa aatcagaagc tggcgttgcg
      301 cggagtacgt gggaacggaa tcacatcacg cacgttttgc acgccagtta cgtaagcaat
      361 caggcgttca aaaccaagac cgaaacctga atgcggaaca gtaccgtagc gacgcagatc
      421 gcgataccac cagtaatctt ctttattcag gcccatttcc agcatacgct cgtccagaac
      481 gtccagacgt tcttcacgct gggagccacc aatgatctca ccgatgcccg gagccagaac
      541 gtccatagcc gcaacggttt taccgtcttc gttgaggcgc atatagaacg ctttaatatc
      601 tttcggatag tttttaacca ctaccggtgc tttaaagtgt tcttccgcca gataacgctc
      661 atgctcagaa gagagatcga cgccccagta aaccgggttt tcaaacttcc tgccgcagtt
      721 ttcgagaatg gtcactgcgt cggtgtaatc cacctgcgcg aaatcggctt caatgaagcg
      781 ttccagacgt gaaacggcat ctttatctac gcgttcagcg aagaatttca tgtcgtctgc
      841 gcgttcttcg agaaccgctt tgaagacata tttcagcatg gcttcagcca gacccgcgat
      901 atcgttcagg ttagcaaacg ccacttccgg ctccagcatc cagaattccg ccaggtgacg
      961 gctggtgttg gagttttcag cacggaaagt cgggccgaag gtataaattt tggataatgc
     1021 gcaagcgtag gtttcgccgt tcaactggcc agatacggtc aggaaagact ctttaccaaa
     1081 gaagtctttg tcgaaatcca ctttgccctg atcgttacgc ggcaggtttt ccagatccag
     1141 cgtagaaacg cggaacattt cgcctgcacc ttcggtatca gatgcggtaa tcagtggcgt
     1201 tgaaacccag aagaatccct gctcgttaaa gaagcgatgc agcgcctgcg ccagcgtatg
     1261 gcgaacgcgc gcgacggcac caatcaggtt tgtgcgcgga cgcaggtgag cgacttcacg
     1321 cagatactca atgctgtggc gttttgccgc catcgggtaa gtgtctggat cttcaaccca
     1381 accagcaact tcaaccttgc tggcctgaat ttcaaattgt tgcccctggc ccggcgatgc
     1441 cacgacttta cccgtcacaa tgaccgagca gccggtggtc agacgcagga cgtcttcatt
     1501 gtaattgggc agagaattat tgatgacagc ctgtacagga tcaaagcagg aaccgtcata
     1561 aacggcgagg aaggagatgc cagcttttga atctcggcgg gtacgtaccc atccgcgtac
     1621 ggtgacttcg ctgtcaacgg ctacgcggcc ctggagtacg tcggctacag gcacaacgct
     1681 cataatattc tctctgttaa tagtcggaaa aaataaacac ttgtccaccc gtaaatgggg
     1741 gggataccta tgttacctgg catctgcaat cagacaagca gaattcgcaa atgcagcgaa
     1801 agatttcgga attaaaggta aaaaagggag ccgattagct ccctgatgat attaactggc
     1861 ttttttaata tgcggaaggt cgaacgcttt gcgcagcgcc cgaacaaacg ctttatcatg
     1921 gcagatagtt ttgccagggc tgtcagaaag tttcgccacc ggtttaccgt tacactctac
     1981 caacttaatc acaatattca ggggttttac ctgggggata tcgcaggtca ggcgagtccc
     2041 aataccaaaa cttaattgca cgcgggaaga gaagtggcgg tatagctcaa ccgctttgcg
     2101 taaatccaga ttgtcagaga aaaccagcgt tttactctgt ggatcaattc ccagcttttc
     2161 ataatgtgca atggcttttt caccccattc aaccgggtcg ccagagtcat gacgcaggcc
     2221 ctgataccga ctagcgaact cgacaccgaa atcacgcagg aaagcatcca tagtgatgca
     2281 gtcggttaat gcaatgccaa gttggtcggg atactcttcc agccaggcag caagtgcagc
     2341 tcgctggctg ttggctagat ccgggctgat ttgctgatgt gcctggaacc attcgtgtgc
     2401 ctgtgttccc atcggcgtga gggaaagccg acgcgccaga tcgtagttgc tggtgcccac
     2461 gaaccaggat tcctgttgca gacgcttaac gatggtttct tgtacttcgc gagaaaaacg
     2521 gcgacgggtg ccaaaatcca tcagatggaa gcgcgacata tcaagaccgg cggttaacgc
     2581 cgagaagtcg actaatttgc tttccagcgt gtcgagggct tgcgcaacgt cggcctgcgg
     2641 tgagcgatag cgatgtacca tttcactgat aaccgccagc aaaggaactt cccagaggat
     2701 gacttcacgc cacggaccgc ttaagcgaat atccagcttg ccattatcgt tggacacggt
     2761 gacttgttcc gggttaaagc ggaactcgcg taaccagtta agatagtcgg ccttaaagaa
     2821 aggcagggca gagagccact gatattcatc atcctgcagg cgcaggtgct gcatcgcctg
     2881 aatctgttca cgaatagcat cggcataaat acccagcaga tcgtcacctc ggcaacgaaa
     2941 ctccgccgcg acatgcacat cgtaatagtg atgaaacacg gcttgctgca tatgcaactt
     3001 ataagcatct gtatccagca acgagtgcag aacaggagaa gcgaattgtg tcataggtgc
     3061 gcagtagcgt cctcgtacag gagcgtttag tacaataaac atcttcagga actgctggag
     3121 tataccttgt ttcgcaattt attgaacccc gatcacacca tatgccacct ttctggtcga
     3181 tggcattacg cggtgtatgt tataaaaatg tagcaataaa ggcgtttgta cctgaaaaga
     3241 tgaagattct gcatagcgcg atttacgcaa caggaataga ctgaacacca gactctataa
     3301 aagatgctaa aggttattta tgactcaaca gccacaagcc aaataccgtc acgattatcg
     3361 tgcgccggat taccagatta ctgatattga cttgaccttt gacctcgacg cgcaaaagac
     3421 ggtcgttacc gcggtcagcc aggctgtccg tcatggtgca tcagatgctc cgcttcgtct
     3481 caacggcgaa gacctcaaac tggtttctgt tcatattaat gatgagccgt ggaccgcctg
     3541 gaaagaagaa gaagagggcg cactggtcat cagtaatttg ccggagcgtt ttacgcttaa
     3601 gatcattaat gaaataagcc tggcggcgaa tacggcgctg gaagggcttt atcagtcagg
     3661 cgatgcgctt tgcacccagt gtgaagccga aggtttccgc catattacgt attatctcga
     3721 ccgcccggac gtgctggcgc gttttaccac caaaattatt gccgataaaa tcaaatatcc
     3781 cttcctgctt tccaatggta accgcgttgc gcaaggcgaa ctcgaaaacg gacgccattg
     3841 ggtacagtgg caggacccgt tcccgaaacc gtgctacctg tttgcgctgg tggcaggcga
     3901 ctttgatgta ctgcgcgata cctttaccac gcgttctggt cgcgaagtag cactggagct
     3961 gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg atgacctcgc tgaaaaactc
     4021 catgaaatgg gatgaagaac gctttggcct ggagtatgac ctcgacatct atatgatcgt
     4081 cgcggtggat ttcttcaata tgggcgcaat ggagaataag ggtctgaata tctttaactc
     4141 caaatatgtg ctggcccgca ccgacaccgc caccgacaaa gattacctcg atattgaacg
     4201 cgttatcggc catgaatatt tccataactg gaccggtaac cgagtgacct gccgcgactg
     4261 gttccagctc agcctgaaag aaggtttaac cgtcttccgc gatcaggaat tcagctctga
     4321 ccttggttcc cgcgcagtta accgcatcaa taatgtgcgc accatgcgcg gattgcagtt
     4381 tgcggaagac gccagcccga tggcgcaccc aatccgcccg gacatggtca ttgagatgaa
     4441 caacttctac accctgaccg tttacgagaa gggcgcggaa gtgatccgta tgatccacac
     4501 cctgctgggc gaagaaaact tccagaaagg gatgcaactc tatttcgagc gtcatgatgg
     4561 tagcgcagcg acctgtgacg actttgtgca ggcgatggaa gatgcgtcga atgttgatct
     4621 ctcccatttc cgccgttggt acagccagtc cggtacgccg gttgtgaccg tcaaagacga
     4681 ctacaatccg gaaactgagc agtacaccct gaccatcagc cagcgcacgc cagccacgcc
     4741 ggatcaggca gaaaaacagc cgctgcatat tccgtttgcc atcgaattgt atgacaacga
     4801 aggtaaagtg atcccgttgc agaaaggcgg tcatccggtg aattccgtgc tgaacgtcac
     4861 ccaggcggaa cagacctttg tttttgataa tgtctacttc cagccggtgc ctgcgctgct
     4921 gtgcgaattc tctgcgccag tgaaactgga atataagtgg agcgatcagc aactgacctt
     4981 cctgatgcgt catgcgcgta atggtttctc ccgttgggat gcggcgcaaa gtttgctggc
     5041 aacctacatc aagctgaacg tcgcccgtca tcagcaaggg cagccgctgt ctctgccggt
     5101 acatgtggct gacgctttcc gcgcggtgct gctcgatgag aagattgatc cggcgctggc
     5161 ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct gaactgttcg atatcataga
     5221 cccgattgct attgccgaag tacgcgaagc actcactcgt actctggcga ctgaactggc
     5281 ggatgagctg ctggctattt acaacgcgaa ttaccagagc gagtaccgtg ttgagcatga
     5341 agatattgca aaacgcactc tgcgtaatgc ctgcctgcgc ttccttgctt ttggtgaaac
     5401 gcatctggct gacgtgctgg tgagcaagca ataccatgaa gcaaacaata tgaccgatgc
     5461 gctggcagcg ctttctgcgg cggttgctgc acagctgcct tgccgtgacg cgctgatgca
     5521 ggagtacgac gataagtggc atcaggacgg tctggtgatg gataaatggt ttatcctgca
     5581 agccaccagc ccggcggcga atgtgctgga gacggtgcgt ggtctgttgc agcatcgctc
     5641 atttaccatg agcaacccga accgtattcg ttcgttgatt ggtgcgtttg cgggcagcaa
     5701 cccggcagcg ttccatgccg aagatggcag cggttaccag ttcctggtgg aaatgcttac
     5761 cgacctcaac agccgtaacc cgcaggtagc ttcacgtctg attgaaccgc tgattcgcct
     5821 gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg gcgctggaac agttgaaagg
     5881 gctggaaaat ctctctggcg atctttacga gaagataact aaagcactgg cttgatactt
     5941 ttgagccaaa atgcctgatg cgacgcttga cgcgtcttat caggcctaca actccgttga
     6001 aattgctggt tttgtaggct ggataaggcg ttcacgccgc atccggcagc cgtgcccaac
     6061 tgcctgatgc gatgcttgac gtgtcttatc aggcctacaa gtccgttgaa aatgctggtt
     6121 ttgtgcgcca catccggcat attaaccatg acgccgaatc agttgttcag aatgcccacg
     6181 ccgcattacc cgttgcaata cttccgcttc cagctccgct aatcgcaccg accctaatcg
     6241 acgcggacgg gggatatcca ctgtcaaatc cagactaatt tttccctctt caattaacaa
     6301 cacccggtca gccattgcta ccgcttcgct cacatcatgc gtcaccaaca gtacggtaaa
     6361 gccgtgctgc tgccaaagtg acacaatcaa atcctgcatc tcgagtcgcg ttaaggcgtc
     6421 cagtgccccg agcggttcat caagcaacaa taaacccggt cgatgaatca acgctcttgc
     6481 cagcgccaca cgctgtttct gcccgccaga aagtgcggca ggccattccc cggcgcgatt
     6541 ctccagtcct gccgcagcca acgcccgacg cgcggcatcg cgccactgac ctttaaggcc
     6601 taacccaacg ttatcaatca ccgatttcca cggcagcaga cgcgcatcct gaaacatcat
     6661 tcgcgtatca tcctgaattt cagccagcgg tgtggtgccc gctaacacat cgcctgcggt
     6721 tggcgtttcc agaccggcca gcaggtgcag cagggtactt ttaccaccac cgctgcggcc
     6781 caccaccgcc acaaactgac ctgccggaat atgtaaatcc agttggttca ggacgatatt
     6841 ttccgcgtaa tgtttgctta ctgcattgag caacaatggt gtgccctggt tcagacgagc
     6901 agtattcata tcgtggcctc cttcaaatga taagccgggt tccagcgcaa ccagaggcgc
     6961 tctaacagct gcgcgctgac gtcagcaagt ttgccgagca gggcgtaaag aataatagcg
     7021 actaccacca cgtctgtttg caaaaactct cgcgcattca tcgccagata accaatgccg
     7081 gaattggcag aaatggtttc ggcaacaatc agcgtcagcc acatcaggcc caacgcaaaa
     7141 cgcacgccga ccataattga gggcagggca tcgggcagga tcacatggat aaacagcggt
     7201 atgccggata atccatagct acgcgccatc tccaccagcc cgcgatcgat attacggatg
     7261 ccatgccagg tgttgatata aatggggaac agcgtaccga gcgccaccag aaagattttc
     7321 gcggattcat caatgccaaa ccacaaaatc accagcggga tcagcgccag atgcggcacg
     7381 ttgcgcaaca tctgaattga ggtatccagc agcctttctc cccagcgcga cagtccgcta
     7441 atcagtccca gaatcaggcc cagcgatccg ccaattgaaa agccaatcag cgcccgccag
     7501 gaactgatcg ccagatgct
//
LOCUS       DAZOZX010000163         7449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZOZX010000163 DAZOZX010000000
VERSION     DAZOZX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.163"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(267..752)
                     /gene="sixA"
                     /locus_tag="ACHUPT_003597"
     CDS             complement(267..752)
                     /gene="sixA"
                     /locus_tag="ACHUPT_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0821004.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(955..3099)
                     /gene="fadJ"
                     /locus_tag="ACHUPT_003598"
     CDS             complement(955..3099)
                     /gene="fadJ"
                     /locus_tag="ACHUPT_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0821005.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3099..4409)
                     /gene="fadI"
                     /locus_tag="ACHUPT_003599"
     CDS             complement(3099..4409)
                     /gene="fadI"
                     /locus_tag="ACHUPT_003599"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0821006.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4589..4873)
                     /locus_tag="ACHUPT_003600"
     CDS             complement(4589..4873)
                     /locus_tag="ACHUPT_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0821007.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5245..6585
                     /gene="fadL"
                     /locus_tag="ACHUPT_003601"
     CDS             5245..6585
                     /gene="fadL"
                     /locus_tag="ACHUPT_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0821008.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6951..>7449
                     /gene="yfdF"
                     /locus_tag="ACHUPT_003602"
     CDS             6951..>7449
                     /gene="yfdF"
                     /locus_tag="ACHUPT_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR0821009.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKKGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKS"
BASE COUNT         1946 a         1932 c         1712 g         1859 t
ORIGIN      
        1 acatcctgcc tgattattca actctatttt ctctattatc tgtattaata aatgacaatg
       61 agtgtcaaac agatattatt catttcaaca tgtaactttg ggcaaaatca agatacgcgt
      121 agaagaataa tttccgaatg aatgtagaaa gtgttttatt tatagataag gagagtcttc
      181 gatagtagtg aggttgacag atatccgctg cctaatgagc aattatgccc aatgaatcta
      241 cctcattggg cataatttgg aacacatcag atagcttttg ccatcttcag attacacggg
      301 ctcatctgcc agttgaatgt accgtttccg ctttcatcaa gcgttacgct ggcaatggcc
      361 gaggtggtaa acataggcgg cgtttcgccc ggacataact cggcaaccag atacccgact
      421 aacggtaagt gagagatcac cagtaccgaa gcaacacctt cattggtcaa cgcctgcaaa
      481 taggcactga ccagaccaac atcgccgcag ggcgttaact ccggcagaac ttctgcactg
      541 gaaggcaggt tcagacaatc ccctacttct tccagtgttt gctcggctcg caggaacggg
      601 ctcaccagaa cgcgttcgat ttccactttt tgacctttca gccagttcgc catcaggcga
      661 gattcgtcac aaccattagt ggtcagagga cgaaccgaat cactggcgga atcgagggct
      721 gcgtcgccgt gacgcatgat aaaaacttgc atattgcacc gcttttgtta accagattcg
      781 cccgttttct ttcatctgaa accagagaaa gaaaacggtg gccggcattg tgccttatcc
      841 attcaccgaa tgaaacgctg ttttttacct caatggcgta agtatagtca atatgcgttt
      901 acattttgac caacactccg ccattcagcg cggattcata tagctttgac cttcttattg
      961 caggtcagtt gcagttgttt tccaaaaact ttccccacgc gcgcccatct cgaccaaacg
     1021 ctcgcaaggg gtaaaacggg aaccatactg cgtggccagt cgttgcatta ttgcaaccac
     1081 ttcgcccgcg ccgagagaat cgatatagcg gaacggtcca ccgagaaatg gcggaaaacc
     1141 aatgccaaat accgcaccaa tatccccgtc acgcacgcta cggataacct gctcatcaac
     1201 acaacgtact gcttcattca gcatcaacat cacacaccgt tcagcaacct gcggtgcgga
     1261 gagtcgcccc tgcccttgtg cgccaatgag cgggtaaatg gcaggatcga cctgtttttt
     1321 gcttttacgc cctttctgac cataaagata gaaaccccga ccattttttc tgcctttgcg
     1381 atcgtcgttc aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc
     1441 ggcttccagt acaggaataa ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg
     1501 gattgggcct accggaaaac caaatttcac tagcgcggca tcaatgtgct ctacccgttc
     1561 accttcggtc agcatgcgga tagcttcatt aatgtaaggc gctaagatgc gattgacgta
     1621 aaacccggct ttgtcacgca cgacaatggg cgttttgccc tgttttttcg ccagttttac
     1681 cgtagtggcg atggtttgcg ccgatgtacc cgcatgagga ataatctcta ccagcggcat
     1741 tttttccacc ggactgaaga aatgcaggcc aataacttgc tcaggtcgcg cggcgtgagc
     1801 ggcgatatca ccaatcggta aagatgacgt gttcgaagca aaaatggtat gagcggcgca
     1861 attttgctcc acttccgcca ccatctgttg ttttaattcg agattttcaa acaccgcttc
     1921 aataatcaga tcgcgatggg caaagccgcg atagtccgtc gttccggaga ttaatgccag
     1981 ctgtttgtca cgttcgctgg ctttgagatg acgacggcga actttgccct ccagctgatc
     2041 ccaactgtac ttcagcgcat gatttatgcc ctgcgggttg atatctttaa ttctgaccgg
     2101 aagccccgct ttacaagcag tgacataagc aataccgccg cccatcaagc caccacctaa
     2161 aattcccacg ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt tcttcacgtc
     2221 cgtactggca aaaaagatac tacgcaaagc ttgcgattgc ggcgtcatcg ccagttcgcc
     2281 aaaggcgcga gcttcagcgt catagccgct gctggtgccc tgcgctaatc ccgtttcaac
     2341 aacctccagg atgcgttctg tcgccggata attgccttga gttttgtgtt ctgttttctt
     2401 gccgaccatt ttgaacagca gcgcacgacc taacggcccc gccagaatac gctcgcgtac
     2461 aggtagaggg cgggaagatg ggcgatcctt cttcgccagc tcaacagcgg cttccagcaa
     2521 aatggagtgc ggaacaacgt catccaccag ccccagcttt aatgcctgtt tcgcccgaag
     2581 ttgttttccg gtgagaatca tctctaatgc tgtactgacg cctatcagac gcggtaaacg
     2641 ctgggtgccg cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt
     2701 tttaggatcg tcagtacaaa cgcgaccgtg gcacgccagc gccagctcca gcccaccgcc
     2761 caggcaagcg ccatgaatag ccgcgataac cggaatgggc aaagcatgaa tctccgccat
     2821 caactgttgg ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc agttgccaat
     2881 catgttgatg tctgctccgg cgataaaatt gtccggtttc gcggagataa acaccacgcc
     2941 gcgcaactct ttgttttcac ggagttgctt aataatggcg cgcacctgcg aggcaaattc
     3001 cgccttcagg gtattcattt tctcatccgg tacgtcgatg gtgatgatag caatgttgtc
     3061 cagacgaaca ttaagagtaa acgctgatgc catttccatt attccgcctc cagaaccatt
     3121 gccgcgccaa gcccaccggc agcacaggcg gtaactaaac caaatccacc gccgcgacgg
     3181 cgaagttcat gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgtccg
     3241 taagcaatcg aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt
     3301 gcacgcccca gtacttcacg agcaaaacgt tcactaccca gcaactgaat attcgccagt
     3361 gtctgagctg caaaggcttc gtgcatatcg atcaatgtca gttcgctcat cgtcaaaccg
     3421 gcacgttcca gcgccagcgg tgttgaccag gctggaccga gcaacatgtc ctgccagaca
     3481 tcaatcgcag taaatgcgta gctgcgcaga taccccagcg gcaccagccc taattctttc
     3541 gcccgggatt cagtcatcag gatcaccgct gccgcgccat cggtcagcgg cgtactgttt
     3601 gccgccgtta ccgttccgtg tttgcgatca aacgccgggc gcagctttgc gtaatcggca
     3661 agcgaggaat taccgcgaat attgttgtct tcgacaagcg gttgtttata aggagggata
     3721 aaggcagtca tcacctcttc tttgagtttt ccgtctgacc atgcctgagc ggcacgctga
     3781 tgcgaacggt gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt tttcgccatt
     3841 tgttcggcgg tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc aggcggtacg
     3901 ggcattaagt cgcgcaaacg caggcgagag aagagtttca ggcgctggct catggttcga
     3961 gctttgttga catcaaccag cacgtacgcc agttttttac tgacgccaat tggcaatacg
     4021 gaagaggaat ctgccccacc ggcaatcccc gctcggatag ttcccgccat caggctttct
     4081 gcgacgtttg caaccgcttg gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg
     4141 gtatgcacat tcattccggt accgagaaca atttcacgcg caatgttggg ggcttcaggc
     4201 atttgtacga cctgaccaaa gaccagctgt tcaatcactt cagcggggat ctcgctgcgt
     4261 gccagcagtt cgcctaccac catcttccct aaatcaaccg tgggaatgcc atgaaaagcc
     4321 gtcgcctggc gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg
     4381 cgggtaacca gtggtaaaac ctgacccata acactcccct gtaaaaaaat aaataaagtg
     4441 gtctgacctg atcatagtct taaccatttt tttacattta gccaagcgga gaaaaaggaa
     4501 agtgggagct atgacacagt gaagacagaa gaaagaaaag aaaacgcccc tgccagcaac
     4561 tgacaggggc gtacgtaccc gagaggaatt aacgcagacc cagctggaag atcagcattt
     4621 cagcttcgca ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac tcttcagtga
     4681 aagtcgggga gattttgcac ggttcggatt tcacgctacg ggatttttca gtcagcgccg
     4741 ccagcgtttg ttctgcttct gcgcggtttg caaacacgcg gctgtaagac gcggtgcagt
     4801 cggagttgtc cataatggtg ccaacatcca tacagcagca aaccggggtt tcatcagcac
     4861 tacatttact catcgttgat ttcctctgta tgtgcaccca aggtgccaga taaacgttgt
     4921 ggatatttta cgcttccgga aagtgctgct ccagttgtta attctgcaaa atcggataag
     4981 tgaccgaaat cacacttaaa aatgatctaa aacaaaattc acccgaatcc gcgagtgcgc
     5041 aacctccaaa ttttgccagc tggatcgcgt ttcttagatc atatttgaaa aaagatggaa
     5101 acatacttgc aacattctag ctggtccgac ctatactctc gccactggtc tgatttctaa
     5161 gatgtacctc agaccctaca cttcgcgctc ctgttacagc acgtaacata gtttgtataa
     5221 aaataaatca ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc
     5281 gcagtcgcag tggcacttat ctccacccag gcctggtcgg caggctttca gttaaacgaa
     5341 ttttcttcct ctggcctggg ccgggcttat tcaggggaag gcgcaattgc cgatgatgca
     5401 ggtaacgtta gccgtaaccc cgcattgatt accatgtttg accgcccgac attttctgcg
     5461 ggtgcggttt atattgaccc ggatgtgaat atcagcggaa cgtctccgtc tggtcgtagc
     5521 ctgaaagccg ataacatcgc gcctacggca tgggttccga acatgcactt tgttgcaccg
     5581 attaacgacc aatttggttg gggcgcttct attacctcta actatggtct ggcaacagag
     5641 tttaatgata cttatgcagg cggctctgtc gggggtacaa ctgaccttga aaccatgaac
     5701 ctgaacttaa gcggtgcgta tcgcttaaat aatgcatgga gctttggtct tggtttcaac
     5761 gccatctacg ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca gttggttgct
     5821 ggtcagatta tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt ggcagctacc
     5881 gccaacggta tcgacagtaa taccaaaatc gctcatctga acggtaacca gtggggcttt
     5941 ggctggaacg ccggaatcct gtatgaactg gataaaaata accgctatgc actgacctac
     6001 cgttctgaag tgaaaattga cttcaaaggt aactacagca gcgatcttaa tcgtgcgttt
     6061 aataactacg gtttgccaat tcctaccgcg acaggtggcg caacgcaatc gggttatctg
     6121 acgctgaacc tgcctgaaat gtgggaagtg tcaggttata accgtgttga tccacagtgg
     6181 gcgattcact atagcctggc ttacaccagc tggagtcagt tccagcagct gaaagcgacc
     6241 tcaaccagtg gcgacacgct gttccagaaa catgaaggct ttaaagatgc ttaccgcatc
     6301 gcgttgggta ccacttatta ctacgatgat aactggacct tccgtaccgg tatcgccttt
     6361 gatgacagcc cagttccggc tcagaatcgt tctatctcca ttccggacca ggaccgtttc
     6421 tggctgagtg caggtacgac ttacgcattt aataaagatg cttcagtcga cgttggtgtt
     6481 tcttatatgc acggtcagag cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa
     6541 ggtaaagcct ggctgttcgg tactaacttt aactacgcgt tctgataacg cgttcgcctg
     6601 gataaagtca cctgctatgc aggtgacttt aactccccca cttcaccgaa agtagtttcc
     6661 tcattgcttg cctcctgagt tttgcaaaca gcctgatggc agcttgccct ttcaaatcag
     6721 taagtggtat ccacagacaa acctgaaggg aaatgcatat tttcaagcgt tctgctcgtc
     6781 cttctcaaag agtttacttt tctgcatttc caggatactc aacccccctg gctattgtgc
     6841 gcttatacac tcaaattaaa gataggttct aaataaatga gcgttttttg atagtctatt
     6901 tcattaggta atatatattt gtaacaaatc aatcaacatg gaataaaatc atgctaccat
     6961 ctatttcaat caacaatacc agcgcagctt acccagaatc catcaatgaa aataacaatg
     7021 atgaaattaa tggattagta caagagttca aaaacctttt taatggtaag aaaggaataa
     7081 gcacctgtat taaacatcta cttgagctta taaaaaacgc catacgagta aacgacgatc
     7141 cttatagatc taatattaat aattcctcag ttacttatat tgatattggc tccaatgata
     7201 cagaccatat tactattggt atcgacaacc aagaaccaat agaattacct gcgaactata
     7261 aagacaaaga actcgtccgt actatcatta atgacaacat agttgagaag actcatgata
     7321 tcaataacaa ggaaatgatc ttcagcgcat taaaagaaat atatgatgga gatcctggtt
     7381 ttatcttcga taagatatca cacaaactca gacatacggt aacggaattt gatgagagcg
     7441 ggaaaagcg
//
LOCUS       DAZOZX010000164         7375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZOZX010000164 DAZOZX010000000
VERSION     DAZOZX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.164"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1541
                     /locus_tag="ACHUPT_003603"
     CDS             <1..1541
                     /locus_tag="ACHUPT_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0821010.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHUPT_003604"
     CDS             1593..2750
                     /locus_tag="ACHUPT_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0821011.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHUPT_003605"
     CDS             complement(2716..2838)
                     /locus_tag="ACHUPT_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821012.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="ACHUPT_003606"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="ACHUPT_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="ACHUPT_003607"
     CDS             4764..7124
                     /locus_tag="ACHUPT_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0821013.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7375
                     /locus_tag="ACHUPT_003608"
     CDS             7181..>7375
                     /locus_tag="ACHUPT_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821014.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2163 a         1615 c         1568 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAZOZX010000165         7363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZOZX010000165 DAZOZX010000000
VERSION     DAZOZX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.165"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUPT_003609"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUPT_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR0821015.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHUPT_003610"
     CDS             complement(1433..1759)
                     /locus_tag="ACHUPT_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR0821016.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHUPT_003611"
     CDS             1894..2235
                     /locus_tag="ACHUPT_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR0821017.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHUPT_003612"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHUPT_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHUPT_003613"
     CDS             complement(2833..3543)
                     /locus_tag="ACHUPT_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR0821018.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHUPT_003614"
     CDS             3651..3956
                     /locus_tag="ACHUPT_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR0821019.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHUPT_003615"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHUPT_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6298..7287)
                     /locus_tag="ACHUPT_003616"
     CDS             complement(6298..7287)
                     /locus_tag="ACHUPT_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821020.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1945 a         1873 c         1762 g         1783 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc cctatcttct attgcactac cgcactagac
     6001 aactagtaca aaacaaaggc ccagaccatt ggtccagacc gtagttagtt aatcacagtg
     6061 acaagccacc acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct
     6121 tacctccctc ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag
     6181 gagctacagg aggagctaca ggaggcggga ccgaagcccc accttgagtc ccaccaccac
     6241 ctgtcaggcc cttggcgatg gacctgaagc ctttactaat gctcttaccc attggtctta
     6301 cgcttcggtc ttggtgaact tcaggcgttt gactacaggc aggacggcag tagctgagcc
     6361 agtgcccatc ttgatggtcg tagcagcttc agcgacaacg aacactttac cgaggatttc
     6421 caagtcgtgg gactgagagt cgccatcacg tgaaaggacg accgtaatat tctcaccgtc
     6481 gtgacggaag cagccgaagc tcagggccgt gaagcgttta gccagcatga atacaccctt
     6541 gtcttcaccg ttgtaatagg cgatgtctgt ccccagcata tcgacgatag cgtcttcacc
     6601 ggagaagcct tgcttagcag ccagacggga cagtgctttc agttggctct catgagcgat
     6661 aataccgtag tcagacacct cagaaccgaa agaagactct gtgttctcat acagagcgtc
     6721 catgatagcg tctgccttag cggtcaggct accagtcagg acaggaagct ctacggtctt
     6781 cagcagctta gaggattcca gaacgtcgac catgcgagcg ttgatggtac ggaccacatt
     6841 ctcacagatg ttcatagcgt cctgctgaag tgtgccctga gtgaatccgg tggatgactt
     6901 ggcgtcccag agcttggtct gctcagagat gagaatagcc tgctcgattg gttcgaccac
     6961 gatttcttgt ccagacggaa cggtcacgac ttcaggtcgc ttgccaccac ggacaggaac
     7021 gaccacagag cggtcagcca gagtgtgagc aatgattggg gtcggtaagc tgttacgggt
     7081 cggcaggact tctacaggga acgggatgtt ctcttgagcg tctttaagta caccgttcag
     7141 catagcctta accgtcttag ctaactggag ggcatagacc tgcgggtcag cgttagtcgg
     7201 cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct caatgccaaa
     7261 ctggaaagcg tctttaatcg tagtcataat aatcaatctc ctaaattagt ttaaatactg
     7321 tgagtcctca aaactgagga gtccctaaca tcgggtcagg gac
//
LOCUS       DAZOZX010000166         7317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZOZX010000166 DAZOZX010000000
VERSION     DAZOZX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.166"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            146..388
                     /gene="yiiF"
                     /locus_tag="ACHUPT_003617"
     CDS             146..388
                     /gene="yiiF"
                     /locus_tag="ACHUPT_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0821021.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(718..1647)
                     /gene="fdhE"
                     /locus_tag="ACHUPT_003618"
     CDS             complement(718..1647)
                     /gene="fdhE"
                     /locus_tag="ACHUPT_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0821022.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1644..2279)
                     /gene="fdoI"
                     /locus_tag="ACHUPT_003619"
     CDS             complement(1644..2279)
                     /gene="fdoI"
                     /locus_tag="ACHUPT_003619"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0821023.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2276..3178)
                     /gene="fdoH"
                     /locus_tag="ACHUPT_003620"
     CDS             complement(2276..3178)
                     /gene="fdoH"
                     /locus_tag="ACHUPT_003620"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0821024.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3191..6241)
                     /gene="fdnG"
                     /locus_tag="ACHUPT_003621"
     CDS             complement(3191..6241)
                     /gene="fdnG"
                     /locus_tag="ACHUPT_003621"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5654..5656),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0821025.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6435..7268
                     /gene="fdhD"
                     /locus_tag="ACHUPT_003622"
     CDS             6435..7268
                     /gene="fdhD"
                     /locus_tag="ACHUPT_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0821026.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHVAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1648 a         1931 c         1966 g         1772 t
ORIGIN      
        1 gaacttataa taactgcaac tgttacatcg tatctggaaa acgcctcgca aaaataaaaa
       61 atgatgcgta aatgagcttt ctttaaagat tcggcaaggg tacttttctc tccatagatt
      121 caaaaatgat attggcgaga tatttatgaa ctctctggca ggtatagata tgggcagaat
      181 tttactcgat ttatcgaatg aggtgattaa gcaacttgat gatctcgagg tgcagcgtaa
      241 tcttcctcgt gcggacctat taagggaagc ggtagatcaa tacctgataa atcaatcgca
      301 aacagcaaga accagtgttc ctggcatctg gcaagggtgt gaggaagatg gtgtcgaata
      361 tcagcgtaag ctgcgcgagg aatggtaatg ggaaaaaatg atgtcaatca gattgctgac
      421 aacgtgcgcg ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaagtcat
      481 gcaaattcaa tatattgaag tttattggta ggcctgataa gcgcagcgca tcaggcaatc
      541 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
      601 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      661 gtcacaccct atgagctgta atccgggcga cctgcgccac ccggaaaacg tcagagatta
      721 ctctccttca cccggaaaca ggaacgggtt aatggaacta cgggcgtagc cttcttgctc
      781 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      841 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      901 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      961 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
     1021 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagagccac ataccgggca
     1081 atattgacgt tgttcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
     1141 cgcccagtag agcgacagcg cagcccagat aaacggcgct ttatcgctgc tgacggacga
     1201 gaaatcagag gcaaacagcg cgctggccat atcttccagc tcctgagtcg atgccttctc
     1261 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaattag
     1321 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
     1381 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
     1441 gtacagcacc actttctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
     1501 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1561 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1621 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1681 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1741 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1801 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1861 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1921 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1981 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     2041 aggttttgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     2101 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     2161 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     2221 atccagtggt tgatacgttc cggcgcggtg tagcgcacga tggtgtcacg tcgtttcatt
     2281 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     2341 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     2401 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     2461 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacatgacg tgtgtaccac
     2521 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2581 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtgcc gaagtgaatc gcgcccgttg
     2641 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2701 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2761 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2821 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2881 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2941 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     3001 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     3061 tacaggtggt aacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     3121 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     3181 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     3241 tgttcgcatc accaacgaat ggcgttaaag tattggcaat aaagcctttt ttagctacgc
     3301 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     3361 gcgtgcgaat acgtttggtc accaccgctt tggctttgat atagccacgg ttggaggaga
     3421 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     3481 caaactgctc tggctgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaagt
     3541 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3601 cggcgtcgtc tttaaagata cgtgcagctg ggttcgagat aacgtttgga tgcagcgggt
     3661 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3721 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3781 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3841 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3901 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3961 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     4021 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     4081 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     4141 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     4201 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     4261 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     4321 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     4381 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     4441 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagt gggtcgatgg
     4501 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4561 caacagggtt aaagccctgg cagatatagc cattgacctt gccctctttc atcatctcga
     4621 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4681 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4741 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4801 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4861 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4921 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4981 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     5041 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     5101 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     5161 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     5221 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     5281 catcttcaaa gccgtaatcc tcacgcacga tcaggctggc gttggtatag gcttcggtgt
     5341 attcgtggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     5401 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     5461 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     5521 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5581 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5641 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5701 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5761 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5821 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5881 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5941 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     6001 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     6061 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     6121 gcagtttata ctgccgggtt tccgcgagtg ctacgctggg tgcaaaaccc agtgccgctg
     6181 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     6241 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     6301 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     6361 tccctccatc taggaagggt ttgtaacaga ataccataat gttggtgtgt atgttcttat
     6421 ctggttaaga gaaagtgaaa aaaacacagc gaaaagaaat cgaaaatgtg acaaatatca
     6481 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6541 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6601 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6661 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6721 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6781 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6841 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6901 atctcaacga ttttcagcca gtggggcgac tgactggttg tactcacgtc gctgcctgga
     6961 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     7021 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     7081 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     7141 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgc tgtaatctga
     7201 cactggtagg tttttgtaaa ccgggtcggg caacggttta tacccatccg cagcgtttga
     7261 gcaattaatt aaaataagtc ctgcgatata tattaaattg caggacttat tcatttc
//
LOCUS       DAZOZX010000167         7288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZOZX010000167 DAZOZX010000000
VERSION     DAZOZX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.167"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..456)
                     /gene="folK"
                     /locus_tag="ACHUPT_003623"
     CDS             complement(<1..456)
                     /gene="folK"
                     /locus_tag="ACHUPT_003623"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0821027.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRA"
     gene            complement(453..1850)
                     /gene="pcnB"
                     /locus_tag="ACHUPT_003624"
     CDS             complement(453..1850)
                     /gene="pcnB"
                     /locus_tag="ACHUPT_003624"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0821028.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1910..2806)
                     /gene="gluQRS"
                     /locus_tag="ACHUPT_003625"
     CDS             complement(1910..2806)
                     /gene="gluQRS"
                     /locus_tag="ACHUPT_003625"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0821029.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2873..3328)
                     /gene="dksA"
                     /locus_tag="ACHUPT_003626"
     CDS             complement(2873..3328)
                     /gene="dksA"
                     /locus_tag="ACHUPT_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0821030.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3506..4210)
                     /gene="sfsA"
                     /locus_tag="ACHUPT_003627"
     CDS             complement(3506..4210)
                     /gene="sfsA"
                     /locus_tag="ACHUPT_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0821031.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4225..4755)
                     /gene="thpR"
                     /locus_tag="ACHUPT_003628"
     CDS             complement(4225..4755)
                     /gene="thpR"
                     /locus_tag="ACHUPT_003628"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0821032.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4829..7258
                     /gene="hrpB"
                     /locus_tag="ACHUPT_003629"
     CDS             4829..7258
                     /gene="hrpB"
                     /locus_tag="ACHUPT_003629"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0821033.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1646 a         1902 c         2058 g         1682 t
ORIGIN      
        1 tgctcttgta tgtaagactt cacgcaacgt ctccccatca ggaaacgcca gctccggtgc
       61 aatctcaaac agcggccaca gcataaatcc acgattcttc atatcgtagt gcggaacggt
      121 caggcgttcg gtatttatca cttcattacc aaacagcatg atatcgagat ccagcgtgcg
      181 tggtccccag cgttcagctt tacgaacacg accctgttgc agttctatgc gctgtgtatg
      241 gttgagtaac tcttccgggg cgggcgaagt ttcaagcgcc acggcggcgt tgaggtaatc
      301 gggttgatct tgcggcccca gcggtggggt gcggtaaaac gaagaaacgg caagaatgcg
      361 gctttcaggg atatcgccta atgctttcag ggcagcattg acctgctcca gcggagaggc
      421 cagattgctg cctatggcaa tatacgccac tgtcatgtgg taccctcacg acgcggtgcg
      481 cgtttgcgtg gacgacgagt acgacgacgc ggtgacggtt cttcatccag ctcgttaagc
      541 atcccttttt gatctggtgg tgcggaaacc tggaactcac cccaccattt caccagacgc
      601 tgcagttcag cattacgttc aacttcagct cgcaaggcca acaggtcata agccgcacgg
      661 aacttaggat gctccagcag tttccatgcg cgtttaccct gacgacggga catacgcaac
      721 tgcaactgcc agatatcgcg ggttaacgtc gtcagacgtt tcgggattgc cagtgaacgg
      781 caggcttcgt ccagcacgtc gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg
      841 ctttcctggg cgatcttctg tgccgtctcc agcagtgggt accagaacat ggcggcaaac
      901 aagaacgccg ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagcacc
      961 tgttcaatga tccgctccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc
     1021 gggaacagcg gctggaacag atgatattca cacaacagct tataggtttc gtaaccgtag
     1081 cccgcttgta gcagtttaag cgattcttca aacaggcgtg ccggtgggat atcgttcagc
     1141 agggtagcga ggcgagggat cggttctgcg gtttccgggc tgatgcgcat acccaatttg
     1201 gcggcaaaac gtaccgcgcg cagcatacgt accggatctt cacggtagcg cgtttccggg
     1261 ttaccaatca gacggataac gccgtccttc agatccttca taccaccaac gtaatcacgg
     1321 acggtaaaat ccgctacgct gtaatacagg ctgttgatag tgaaatcgcg gcgctgggcg
     1381 tcttcttcga tggagccgaa aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg
     1441 gaggtcgtgc ggtcgctgac gttaccttcg tggtgtccac ggaaggtcgc aacttcgata
     1501 atctccgggc caaacattac atgagccaga cggaaacggc gacccaccag gcggcagtta
     1561 cggaacagtt tgcgcacctg ctcaggcgtg gcgttagtgg ttacgtcaaa atctttcggc
     1621 tttttgccaa gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct
     1681 ttgttgagcc tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca
     1741 tgctgctcac gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt
     1801 tcctcgcggc ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc
     1861 ggtagtgtca aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg
     1921 catttgagaa tgttgaattt acaattgccg actcgggcac ggcggtcagc atccagtttt
     1981 tgacggctga ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgct tctgcctgct
     2041 gccccagaaa ttgaagtgcc gcgattagta ccgggcgtgg atcgcctttc ggcaacgcag
     2101 gcgcatgatt ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggcagatgaa
     2161 tgtaatctgg cactttccag ccaaaaagct ggtacagcgg gatttgcctt actgttggtt
     2221 caatcagatc agccccacgc actatttctg taacgccctg gaaatgatca tcaaccacaa
     2281 cagccaggtt gtaggcgaac aacccatcac ggcgatgaat gataaaatct tcccgtgcca
     2341 gtttttcgtc ggcgtgaata atgcctcgca gcaggtcagt aaattgcgtg accggatgct
     2401 gctggcggat acgcactgcg gcgttgtctg gtccatgatg caacacccgg caatgaccgt
     2461 cgtaaatacc gccaatgctt tgaatacgcg cacgcgtaca ggtgcagtaa taacttagtc
     2521 cttgttcatg taaccaggcg agtgcttcac gataggcgtc gtgacgttgc gattgccaga
     2581 gaacatcgcc gtcccagtgc agaccgtaat gttccagctg gcgcaggata gtttctgcgg
     2641 caccgggaac ttcacgaggc gggtcgatat cttctatgcg taccagccag cgaccttgcc
     2701 gggcgcgagc ctgcaaatag ctgccgagcg cggcgatcag agagccaaaa tgaagctcgc
     2761 cggaaggaga gggggcgaag cggccaatat actgtgtgtc tgtcatctct ttgaacaaaa
     2821 aataaggcgg gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag
     2881 ccatctgttt ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg
     2941 ggcgcgcttc cagacggcga ataccaattt caacaccgca ggattcgcag tagccgaaat
     3001 cttcgtcttc cacttttttc agcgtcttct cgatcttttt gatcagctta cgctcgcgat
     3061 cgcggttacg cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg
     3121 ggaagttggc tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt
     3181 gattacgcca tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact
     3241 cttcgcccgg cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg
     3301 atgttttacg gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc
     3361 catgttcggg ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta
     3421 tctaaacgta acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat
     3481 gtaaattaac cagttactta ctttactaca atgtaaccgg cagtgatttt ttaagagcca
     3541 tgccttcagc agaaatttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt
     3601 ctgacaatag ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg
     3661 ctgaatgcag cacggcgaaa aaaataaccg cacgctggcc ttcagccgct acgctcatca
     3721 actcccgaag gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt
     3781 tctccgctaa cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca
     3841 acataaagtc aatacggctg cgttcggagc cgtattttac ttcgcttttc agcgagctat
     3901 agcctgacag ttctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc
     3961 aaagcgtatt gacgcaaata aatgcgccgc tctggctttg agttaattcc caggtgtgtg
     4021 ggtatttccg tttggtgttg tctgaagtcg aataccagac ggtatcgcca ggcgttgcac
     4081 aaccggtcat cgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga
     4141 tcacatcggc taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agagggggag
     4201 aaaattccat tgcgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta
     4261 gcgtgtgcgt ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgcataaga
     4321 ccagttaaaa cccggcggcg ggaatgtcac cgcctcgctg gcgtcgcgca ataaggtaat
     4381 atgtggatga aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag
     4441 catattcgcc agctggatta agccgcgtgg aggctgacgc atccctaacc acaccacacg
     4501 cgaacgcagc cattgtccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg
     4561 tccggctaaa agagaaagcg ccttctcttt ctctgcgctc acttcgccta aaaatgccag
     4621 agtcaggtgc aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc
     4681 gcgccaatgg ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagacg
     4741 ttgcggttca gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc
     4801 gaagaatgtt aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc
     4861 tgaattactt accgctctcg attgtgcgcc gcaggtatta ttaagtgcgc cgaccggggc
     4921 cgggaaatca acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat
     4981 tatcctgctg gagccgcgtc gtctggcggc gcgtaacgtc gcgcaacggc tggcggagct
     5041 gcttaacgaa aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg
     5101 gctgaatacc cgcctggaag tggttaccga aggcgtgctg acgcggatga tccagcgtga
     5161 cccggaactg agcggtgttg gattggtgat ccttgatgag tttcatgagc gcagtttgca
     5221 ggcggatttg gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa
     5281 actgctgatt atgtcggcta cgctggacaa tgaccgcttg cagcaaatgc tgccggaagc
     5341 gcccgtcgtc atctcagaag ggcgctcgtt tccggttgaa cgccgttatt taccgctgcc
     5401 cgcgcatcag cgttttgacg aagccgttgc ggtagccacc gccgaaatgg tgcgtcagga
     5461 aagtggatca ttactgttat ttttacctgg cgtcggagaa attcagcgcg tgcaggaaca
     5521 actggcttcg cgcatcggca gtgatgtggt gctctgcccg ctgtatggcg tgttgtcact
     5581 gaacgatcag cgaaaagcga tcctcccggc accgcaaggg atgcgcaaag tggtgctggc
     5641 gaccaatatt gctgaaacca gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc
     5701 ccaggagcgt gtggcgcgct ttgatccgcg cacggggctt acgcggttga ttactcaacg
     5761 cgttagtcag gcatccatga cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat
     5821 cagcctgcat ttaatcgcca aagaacaagc agaacgcgcc gcggcgcaaa gtgagccgaa
     5881 gatcttacaa agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga
     5941 tccggcgcag atgagctggc tggatcaacc gccaacggtg aatctactgg ccgcgaaacg
     6001 cctgttacaa atgctggggg cgctggaggg tgaacggctt agtgcgcaag ggcaaaaaat
     6061 ggcagcgctg ggtaacgatc cgcgtttagc ggcaatgctg gttaacgcaa agagcgatga
     6121 cgaagctgct accgcggcaa aaattgccgc cattctcgaa gagccgccac ggatgggcaa
     6181 tagtgacctg ggcgtggcgt tttcgcgcaa tcaaccagcc tggcagcaac gtagtcagca
     6241 actgttaaaa cgcttaaacg tacgcggcgg tgaggcagac agttcgctta tcgcgccgct
     6301 acttgccggg gcgtttgccg atcgcattgc tcatcgccgt gggcaagatg gacgctatca
     6361 actggcaaac agcatgggag cgatgctcga tgccgacgac gcgctaagcc gccacgaatg
     6421 gttgatcgca ccgttattat tgcagggcag cgcctcgccg gatgcgcgga ttttactggc
     6481 gctaccggtc gatattgatg aattagtaca acgctgtccg cagctggtgc agcaatctga
     6541 caccgtggaa tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tacaaatcgg
     6601 tcagttgacg gtgaaagtgc agccgctggc gaaaccgtca gaagacgagt tgcatcaggc
     6661 gatgcttaat ggcatccgtg ataaaggttt aagcgtgctc aactggacgg cggaagcgga
     6721 acagctacgc ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc
     6781 ggttgatgat gaaagtttgt tggcgacgct ggaaacgtgg ctgctgccac atatgaccgg
     6841 cgtacattca ctacgcggcc tgaaatcact cgacatttat caggcactac gcggattact
     6901 tgattgggga atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac
     6961 gggaagccgg atcgccattc gttatcatga agataacccg cccgcgctgg cggtgagaat
     7021 gcaggagatg tttggtgagg ccactaatcc gacgatcgcc caggggcgcg tgccgctggt
     7081 gctgaagttg ctttcccctg cccaaaggcc gctgcaaatc acgcgtgatt tgggggcctt
     7141 ctggaaaggg gcgtatcgtg aggtgcaaaa ggagatgaaa gggcgttatc ccaaacacgt
     7201 ctggccggac gacccggcaa acacggcacc gacgcgacgg acgaaaaagt attcgtaagg
     7261 ttggttttct ccctctccct gtgggaga
//
LOCUS       DAZOZX010000168         7228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZOZX010000168 DAZOZX010000000
VERSION     DAZOZX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.169"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHUPT_003630"
     CDS             complement(<1..190)
                     /locus_tag="ACHUPT_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0821034.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACHUPT_003631"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACHUPT_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0821035.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACHUPT_003632"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACHUPT_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0821036.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACHUPT_003633"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACHUPT_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0821037.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACHUPT_003634"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACHUPT_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACHUPT_003635"
     CDS             complement(3262..3381)
                     /locus_tag="ACHUPT_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0821038.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUPT_003636"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUPT_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0821039.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUPT_003637"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUPT_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR0821040.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACHUPT_003638"
     CDS             3803..4147
                     /locus_tag="ACHUPT_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0821041.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACHUPT_003639"
     CDS             4151..4486
                     /locus_tag="ACHUPT_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0821042.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACHUPT_003640"
     CDS             complement(4543..6864)
                     /locus_tag="ACHUPT_003640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0821043.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACHUPT_003641"
     CDS             complement(6981..7190)
                     /locus_tag="ACHUPT_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821044.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         1960 a         1718 c         1610 g         1940 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcct
//
LOCUS       DAZOZX010000169         7133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZOZX010000169 DAZOZX010000000
VERSION     DAZOZX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.170"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..144)
                     /locus_tag="ACHUPT_003642"
     CDS             complement(<1..144)
                     /locus_tag="ACHUPT_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821045.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFS"
     gene            222..1316
                     /gene="ddlA"
                     /locus_tag="ACHUPT_003643"
     CDS             222..1316
                     /gene="ddlA"
                     /locus_tag="ACHUPT_003643"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0821046.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1340..1552)
                     /locus_tag="ACHUPT_003644"
     CDS             complement(1340..1552)
                     /locus_tag="ACHUPT_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0821047.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1812..2120
                     /locus_tag="ACHUPT_003645"
     CDS             1812..2120
                     /locus_tag="ACHUPT_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0821048.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="ACHUPT_003646"
                     /pseudo
     CDS             complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="ACHUPT_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="ACHUPT_003647"
     CDS             complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="ACHUPT_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0821049.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4858..6015
                     /gene="ampH"
                     /locus_tag="ACHUPT_003648"
     CDS             4858..6015
                     /gene="ampH"
                     /locus_tag="ACHUPT_003648"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0821050.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6016..6519)
                     /locus_tag="ACHUPT_003649"
     CDS             complement(6016..6519)
                     /locus_tag="ACHUPT_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821051.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6607..>7133)
                     /locus_tag="ACHUPT_003650"
     CDS             complement(6607..>7133)
                     /locus_tag="ACHUPT_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0821052.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1809 a         1824 c         1725 g         1775 t
ORIGIN      
        1 agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc cgttcatcat
       61 ttttgtgatc ttgcgcacat ctttgagata acgcactttc ttcaccagta cgttgcacgc
      121 tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg ttttgtcgtg
      181 gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag
      241 gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca
      301 ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag
      361 ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata
      421 ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat gagcatcagc
      481 ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc
      541 acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt
      601 ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc accaaacgtc
      661 tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc
      721 acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt gtaaaaccgg
      781 ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag tacgcaattg
      841 ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc
      901 gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga
      961 tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga
     1021 aagtggtagt tccggcagcc attgcaccag agataaacga taagatccgg gcgattgccg
     1081 ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc
     1141 cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta
     1201 tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc acacgtttga
     1261 ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca atgtaatatt
     1321 ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa
     1381 tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc
     1441 aaccttttgc ttcaaatccc agtaccccca ccacgccatt aagaatgaat ataaggccaa
     1501 tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc atggcagctc
     1561 cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca
     1621 ttttcgtaat tattaataaa tttaacttaa ggttgccatt ttgtgactca caacgaagat
     1681 tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg acaatctaaa
     1741 tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc
     1801 cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca
     1861 atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg
     1921 ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc
     1981 aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg
     2041 ggctgatccc gtttttagca ggctgcctca tttttgcagt ggtggcgcta tggctgcact
     2101 ggcgacatcg ccgccagtaa aatttacgtt gccagatgcg gcctgaaacg ccttatctgg
     2161 cctgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt
     2221 cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt gaatgccagg
     2281 caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat aaactttctt
     2341 gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac ggcgaatttc
     2401 gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg
     2461 ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt
     2521 actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc
     2581 aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt aagtaccaaa
     2641 ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca ttttccacgg
     2701 ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg
     2761 caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga tcagatcgtc
     2821 aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc tataactttt
     2881 gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg
     2941 ttcagcacga cggtcaatct cttgctaggc gatcttgctt aagcccggaa cggccggatc
     3001 tgcctgataa tttgactcct gctgcgccac cgccagcacc gagtagacgt tttccagcgt
     3061 cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg catcgcggtc
     3121 tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg ctttttcgcc
     3181 ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa ccgccaacaa
     3241 tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc tcaaggtatg
     3301 ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca gacgtttgta
     3361 gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg
     3421 aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg taccggcaac
     3481 aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat agagaatgcg
     3541 ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc gtacggcgct
     3601 aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag
     3661 ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg gcagtttgat
     3721 ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg caatcaccag
     3781 accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga gcgttaccag
     3841 caccggcagg aaggcgatca acgtcatgat ggcgttgata aaactgacgc ccatattctc
     3901 cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac cttcgatatg
     3961 acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac
     4021 gtagtgactg acaaagaagt tgttcagtac actgatcacc accgcgatca gcgcaatccc
     4081 cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg gcgaacttag
     4141 cgcggtttga atcagatcat agaacggcgc ataccaggcg ttgacggcga ccccgacttc
     4201 caccaaaaac caagtgacga agataatcag cgcagtaccg agtattgacc agtattgcca
     4261 acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta
     4321 agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc
     4381 ggaagcgccg gtgatacgtg ccacccagtc acccccaccg gcttgccaga agataacggc
     4441 aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc ggctttggga aaaaagactt
     4501 aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat
     4561 gaccgctaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc aaggcgctta
     4621 gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa gattgtttac
     4681 ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc
     4741 gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc
     4801 atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac accaccgttg
     4861 aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg
     4921 gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta tgccgatcat
     4981 attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg taaccagcgc
     5041 gtctttcgta gttatggtga aacgcgacct ggtaataacg ttcgcccgca gctggattcg
     5101 gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt gaaattgctc
     5161 gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc
     5221 gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca taccagcgcc
     5281 ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc
     5341 gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg
     5401 gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc gtccggcaag
     5461 ccttataccc agttgtttga agagcagatt acccatccat tggggatgaa agacaccacc
     5521 tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg
     5581 tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc tggcgatatg
     5641 atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggcagat
     5701 cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg catggatgtc
     5761 cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt
     5821 ccgaggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat ggcgatgatc
     5881 ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa
     5941 aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa accgttggtt
     6001 atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt
     6061 attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga cgacagaacg
     6121 cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc gcaattcttc
     6181 attccagtca atcattgata ataatgttgc gcgggtttgt ttggctggta gatgatatcc
     6241 cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc
     6301 cagccccata atataaggag cctgggtaat accgataagt acgttttctt ctctacgcag
     6361 agagataact ccttctaaaa taacgaaagt atcttcgtta ttgacatcac tggaaaatat
     6421 aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac atttatcgag
     6481 cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct ttgcagtaat
     6541 gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc ttcggagtga
     6601 agaatattac caagtatatt taacacccac gttcgcggac cagtcttgat ctacgtcacc
     6661 accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt
     6721 acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac
     6781 atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc
     6841 ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct
     6901 gtcgtaaggc tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat caccagactg
     6961 gaacagacca gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc
     7021 gtaaccggtt ttcaaaccga agccccaagc gtcggagttg gtgctaccgt caacgtaagt
     7081 accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca agc
//
LOCUS       DAZOZX010000170         7103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZOZX010000170 DAZOZX010000000
VERSION     DAZOZX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.171"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            205..1218
                     /gene="intI1"
                     /locus_tag="ACHUPT_003651"
     CDS             205..1218
                     /gene="intI1"
                     /locus_tag="ACHUPT_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0821053.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1421..1771
                     /locus_tag="ACHUPT_003652"
                     /pseudo
     CDS             1421..1771
                     /locus_tag="ACHUPT_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            1897..2457
                     /locus_tag="ACHUPT_003653"
     CDS             1897..2457
                     /locus_tag="ACHUPT_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0821054.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            2460..5426
                     /locus_tag="ACHUPT_003654"
     CDS             2460..5426
                     /locus_tag="ACHUPT_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR0821055.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            5493..5870
                     /locus_tag="ACHUPT_003655"
     CDS             5493..5870
                     /locus_tag="ACHUPT_003655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0821056.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(6071..6730)
                     /locus_tag="ACHUPT_003656"
     CDS             complement(6071..6730)
                     /locus_tag="ACHUPT_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR0821057.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            7009..>7103
                     /locus_tag="ACHUPT_003657"
     CDS             7009..>7103
                     /locus_tag="ACHUPT_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0821058.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYL"
BASE COUNT         1532 a         2114 c         1991 g         1466 t
ORIGIN      
        1 gtattggttt ggtaaagggc ttaattttaa cggctaacaa ttaatgaggc tccgggttcg
       61 cccaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
      121 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
      181 ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
      241 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga
      301 accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
      361 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
      421 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
      481 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
      541 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
      601 catcgtttgt tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa
      661 ctgcgggtca aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc
      721 tccaaggatc gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcagctg
      781 tcgcgtgcac gggcatggtg gctgaaggac caggccgagg gccgcagcgg cgttgcgctt
      841 cccgacgccc ttgagcggaa gtatccgcgc gccgggcatt cctggccgtg gttctgggtt
      901 tttgcgcagc acacgcattc gaccgatcca cggagcggtg tcgtgcgtcg ccatcacatg
      961 tatgaccaga cctttcagcg cgccttcaaa cgtgccgtag aacaagcagg catcacgaag
     1021 cccgccacac cgcacaccct ccgccactcg ttcgcgacgg ccttgctccg cagcggttac
     1081 gacattcgaa ccgtgcagga tctgctcggc cattccgacg tctctacgac gatgatttac
     1141 acgcatgtgc tgaaagttgg cggtgccgga gtgcgctcac cgcttgatgc gctgccgccc
     1201 ctcactagtg agaggtaggg cagcgcaagt caatcctggc ggattcacta cccctgcgcg
     1261 aaggccatcg gtgccgcatc gaacggccgg ttgcggaaag tcctccctgc gtccgctgat
     1321 ggccggcagc agcccgtcgt tgcctgatgg atccaacccc tccgctgcta tagtgcagtc
     1381 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca atggaggtgg tagccgaggg
     1441 tgtggaaaca cccgactgcc ttgcgtggtt gcggcaggcg ggttgcgaca cggtgcaggg
     1501 tttcctgttc gccaggccga tgccggcggc ggccttcgtc ggcttcgtca accaatggag
     1561 gaacaccacc atgaacgcca atgaaccgag caccagttgc tgcgtgtgct gcaaggaaat
     1621 cccgctcgat gccgccttca cgccggaagg ggccgagtac gtggagcatt tctgcgggct
     1681 ggagtgctat cagcgcttcc aggcgcgggc cagcactgcg accgaaacca gcgtcaaacc
     1741 ggacgcttgt gattcgccgc cgtcaggttg aggcataccc taacctgatg tcagatgcca
     1801 tgtgtaaatt gcgtcaggat aggattgaat tttgaattta ttgacatatc tcgttgaagg
     1861 tcatagagtc ttccctgaca ttttgcaggg aattccatga ctggacagcg cattgggtat
     1921 atcagggtca gcaccttcga ccagaacccg gaacggcaac tggaaggcgt caaggttgat
     1981 cgcgctttta gcgacaaggc atccggcaag gatgtcaagc gtccgcaact ggaagcgctg
     2041 ataagcttcg cccgcaccgg cgacaccgtg gtggtgcata gcatggatcg cctggcgcgc
     2101 aatctcgatg atttgcgccg gatcgtgcaa acgctgacac aacgcggcgt gcatatcgaa
     2161 ttcgtcaagg aacacctcag ttttactggc gaagactctc cgatggcgaa cctgatgctc
     2221 tcggtgatgg gcgcgttcgc cgagttcgag cgcgccctga tccgcgagcg tcagcgcgag
     2281 ggtattgcgc tcgccaagca acgcggggct taccgtggca ggaagaaatc cctgtcgtct
     2341 gagcgtattg ccgaactgcg ccaacgtgtc gaggctggcg agcaaaagac caagcttgct
     2401 cgtgaattcg gaatcagtcg cgaaaccctg tatcaatact tgagaacgga tcagtaaata
     2461 tgccacgtcg ttccatcctg tccgccgccg agcgggaaag cctgctggcg ttgccggact
     2521 ccaaggacga cctgatccga cattacacat tcaacgatac cgacctctcg atcatccgac
     2581 agcggcgcgg gccagccaat cggctgggct tcgcggtgca gctctgttac ctgcgctttc
     2641 ccggcgtcat cctgggcgtc gatgaactac cgttcccgcc cttgttgaag ctggtcgccg
     2701 accagctcaa ggtcggcgtc gaaagctgga acgagtacgg ccagcgggag cagacccggc
     2761 gcgagcacct gagcgagctg caaaccgtgt tcggtttccg gcccttcacc atgagccatt
     2821 accggcaggc cgtccagatg ctgaccgagc tggcgatgca aaccgacaaa ggcatcgtgc
     2881 tggccagcgc cttgatcggg cacctgcggc ggcagtcggt cattctgccc gccctcaacg
     2941 ccgtcgagcg ggcgagtgcc gaggcgatca cccgtgctaa ccggcgcatc tacgacgcct
     3001 tggccgaacc actggcggac gcgcatcgcc gccgcctcga cgatctgctc aagcgccggg
     3061 acaacggcaa gacgacctgg ttggcttggt tgcgccagtc tccggccaag ccaaattcgc
     3121 ggcatatgct ggaacacatc gaacgcctca aggcatggca ggcactcgat ctgcctaccg
     3181 gcatcgagcg gctggttcac cagaaccgcc tgctcaagat tgcccgcgag ggcggccaga
     3241 tgacacccgc cgacctggcc aaattcgagc cgcaacggcg ctacgccact ctcgtggcgc
     3301 tggccaccga gggcatggcc accgtcaccg acgaaatcat cgacctgcac gaccgcatcc
     3361 tgggtaagct gtttaacgct gccaagaata agcatcagca gcagttccag gcgtcaggca
     3421 aggccatcaa cgccaaggta cgtctgtacg ggcgcatcgg tcaggcgctg atcgacgcca
     3481 agcaatcagg ccgcgatgcg tttgccgcca tcgaggccgt catgtcctgg gattcctttg
     3541 ccgagagcgt caccgaggcg cagaagctcg cgcaacccga tgacttcgat ttcctgcatc
     3601 gcatcggcga gagctacgcc accctgcgcc gctatgcacc ggaattcctt gccgtgctca
     3661 agctgcgggc cgcgcccgcc gccaaaaacg tgcttgatgc cattgaggtg ctgcgcggca
     3721 tgaacaccga caacgcccgc aagctgccag ccgatgcacc gaccggcttc atcaagccgc
     3781 gctggcagaa actggtgatg accgacgccg gcatcgaccg gcgctactac gaactgtgcg
     3841 cgctgtccga gttgaagaac tccctgcgct cgggcgacat ctgggtgcag ggttcacgcc
     3901 agttcaagga cttcgaggac tacctggtac cgcccgagaa gttcaccagc ctcaagcagt
     3961 ccagcgaatt gccgctggcc gtggccaccg actgcgaaca atatctgcat gagcggctga
     4021 cgctgctgga agcacaactt gccaccgtca accgcatggc ggcagccaac gacctgccgg
     4081 atgccatcat caccgagtcg ggcttgaaga tcacgccgct ggatgcggcg gtgcccgaca
     4141 ccgcgcaggc gctgatagac cagacagcca tggtcctgcc gcacgtcaag atcaccgaac
     4201 tgctgctcga agtcgatgag tggacgggct tcacccggca cttcacgcac ttgaaatcgg
     4261 gcgatctggc caaggacaag aacctgttgt tgaccacgat cctggccgac gcgatcaacc
     4321 tgggcctgac caagatggcc gagtcctgcc ccggcacgac ctacgcgaag ctcgcttggc
     4381 tgcaagcctg gcatacccgc gacgaaacgt actcgacagc gttggctgaa ctggtcaacg
     4441 ctcagtttcg gcatcccttt gccgggcact ggggcgatgg caccacatca tcatcggacg
     4501 gacagaattt ccgaaccgct agcaaggcaa agagcacggg gcacatcaac ccaaaatatg
     4561 gcagcagccc aggacggact ttctacaccc acatctccga ccaatacgcg ccattccaca
     4621 ccaaggtggt caatgtcggc ctgcgcgact caacctacgt gctcgacggc ctgctgtacc
     4681 acgaatccga cctgcggatc gaggagcact acaccgacac ggcgggcttc accgatcacg
     4741 tcttcgccct gatgcacctc ttgggcttcc gcttcgcgcc gcgcatccgc gacctgggcg
     4801 acaccaagct ctacatcccg aagggcgatg ccgcctatga cgcgctcaag ccgatgatcg
     4861 gcggcacgct caacatcaag cacgtccgcg cccattggga cgaaatcctg cggctggcca
     4921 cctcgatcaa gcagggcacg gtgacggcct cgctgatgct caggaaactc ggcagctacc
     4981 cgcgccagaa cggcttggcc gtcgcgctgc gcgagttggg ccgcatcgag cgcacgctgt
     5041 tcatcctcga ctggctgcaa agcgtcgagc tacgccgccg cgtgcatgcc gggctgaaca
     5101 agggcgaggc gcgcaatgcg ctggcccgtg ccgtgttctt caaccgcctt ggtgaaatcc
     5161 gtgaccgcag tttcgagcag cagcgctacc gggccagcgg cctcaacctg gtgacggcgg
     5221 ccatcgtgct gtggaacacg gtctacctgg agcgtgcggc gcatgcgttg cgcggcaatg
     5281 gtcatgccgt cgatgactcg ctattgcagt acctgtcgcc actcggctgg gagcacatca
     5341 acctgaccgg tgattaccta tggcgcagca gcgccaagat cggcgcgggg aagttcaggc
     5401 cgctacggcc tctgcaaccg gcttagcgtg ctttattttc cgttttctga gacgacccct
     5461 attatggcga aggtatcatt caggaagcgt tgatgaagga ttatctctgt aaaaagttat
     5521 tcaaccggct ttccggtacc ctggtgatca gagcgcgctg cggcaataac attactggcc
     5581 tggcatgttg caatattctt tatccctcgc cccgatacag cggtcagctg catattaaag
     5641 agctgtatgt ttctcagtgc gatcgaaata agggcacggg taaagcgata atgcgcttta
     5701 tagcccggct tgcgcttgaa caggaatgcc ttagccttag ctggaacgct gaaaaatcca
     5761 accccggcgc taaccgtttt tatcaggctc tgggaggcag aataaatgat catatcgtca
     5821 attattacct ccacggggag agcctgagca aactggcctc aggcatttga gaagcacacg
     5881 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     5941 taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca
     6001 tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc
     6061 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     6121 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     6181 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     6241 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     6301 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     6361 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     6421 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     6481 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     6541 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     6601 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     6661 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     6721 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     6781 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     6841 attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt
     6901 attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg
     6961 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt
     7021 tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc
     7081 gccggacatc agcgctatct ctg
//
LOCUS       DAZOZX010000171         7030 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZOZX010000171 DAZOZX010000000
VERSION     DAZOZX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.173"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUPT_003658"
     CDS             complement(<1..815)
                     /locus_tag="ACHUPT_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0821059.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUPT_003659"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUPT_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUPT_003660"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUPT_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1874)
                     /locus_tag="ACHUPT_003661"
     CDS             complement(1398..1874)
                     /locus_tag="ACHUPT_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0821060.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHUPT_003662"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHUPT_003662"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0821061.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="ACHUPT_003663"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="ACHUPT_003663"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0821062.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="ACHUPT_003664"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="ACHUPT_003664"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0821063.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="ACHUPT_003665"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="ACHUPT_003665"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0821064.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="ACHUPT_003666"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="ACHUPT_003666"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0821065.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1704 a         1761 c         1948 g         1617 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg
//
LOCUS       DAZOZX010000172         6916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZOZX010000172 DAZOZX010000000
VERSION     DAZOZX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.175"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="ACHUPT_003667"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="ACHUPT_003667"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0821066.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHUPT_003668"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHUPT_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0821067.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHUPT_003669"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHUPT_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0821068.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHUPT_003670"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHUPT_003670"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0821069.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="ACHUPT_003671"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="ACHUPT_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHUPT_003672"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHUPT_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0821070.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="ACHUPT_003673"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="ACHUPT_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR0821071.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1949 a         1575 c         1457 g         1935 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga accccc
//
LOCUS       DAZOZX010000173         6597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZOZX010000173 DAZOZX010000000
VERSION     DAZOZX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.176"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1076
                     /locus_tag="ACHUPT_003674"
     CDS             <1..1076
                     /locus_tag="ACHUPT_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR0821072.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHUPT_003675"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHUPT_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0821073.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHUPT_003676"
     CDS             complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHUPT_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0821074.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="ACHUPT_003677"
     CDS             1897..2343
                     /locus_tag="ACHUPT_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0821075.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHUPT_003678"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHUPT_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="ACHUPT_003679"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="ACHUPT_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="ACHUPT_003680"
     CDS             4450..4797
                     /locus_tag="ACHUPT_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0821076.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHUPT_003681"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHUPT_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0821077.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHUPT_003682"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHUPT_003682"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0821078.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="ACHUPT_003683"
     CDS             6314..6412
                     /locus_tag="ACHUPT_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0821079.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHUPT_003684"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHUPT_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0821080.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1599 a         1599 c         1615 g         1784 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataa
//
LOCUS       DAZOZX010000174         6529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZOZX010000174 DAZOZX010000000
VERSION     DAZOZX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.177"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..201
                     /locus_tag="ACHUPT_003685"
     CDS             <1..201
                     /locus_tag="ACHUPT_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR0821081.1"
                     /translation="NKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQGAELINKA
                     KHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(268..1980)
                     /locus_tag="ACHUPT_003686"
     CDS             complement(268..1980)
                     /locus_tag="ACHUPT_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFR0821082.1"
                     /translation="MPEQLYNVVVSQPSQLFTLARSFKANANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQFY
                     FPNLLKYDPDQLKQLLQSDEGATYIGTNSGKNLQDELFAIKNEIYQDPLTSWPFGGTL
                     KIKQGVYDVTTPLVLNYDDYPADFVGAPGVRCFYSGENMAETILECRATDFNVKMLGD
                     AAFNSQRLAAYDYIGNMTLKGNPGSYGMLVQNKAHTRLENIVICRHPDGEGLRTDSML
                     TSDMTNVYLQSNRIGWRATNSVNHSELNAITANRLTCSQNSEWGILGDRWGAGTTINS
                     LTCEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADLAIDNTGTRPVTV
                     VINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGA
                     NCEVVDIGCVFTETTSKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVS
                     FSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSI
                     TRLL"
     gene            2045..2503
                     /locus_tag="ACHUPT_003687"
     CDS             2045..2503
                     /locus_tag="ACHUPT_003687"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0821083.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            2635..2895
                     /locus_tag="ACHUPT_003688"
     CDS             2635..2895
                     /locus_tag="ACHUPT_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFR0821084.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(2985..4982)
                     /locus_tag="ACHUPT_003689"
     CDS             complement(2985..4982)
                     /locus_tag="ACHUPT_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0821085.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(4982..6277)
                     /locus_tag="ACHUPT_003690"
     CDS             complement(4982..6277)
                     /locus_tag="ACHUPT_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR0821086.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNNVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALT
                     ARGQDIQIRGQNISAQNAALSREIQRAELQEKALDRQIARESNQLKLEELKQKQADVR
                     QKAEIARADRQAAAQGAVDTFSTALDSLNEIEQSPGLSKAVGIRSAFPTVPGSDAANF
                     EARLDTFKAQTFLPMVQSLKGMGALSDAEGKKLSDAVGALSPKMSEKAFRDSIGKIRN
                     QLESKLSTVKKQFDYQEPVQNTPGQQSPAGSNFSSLWGD"
     gene            complement(6287..>6529)
                     /locus_tag="ACHUPT_003691"
     CDS             complement(6287..>6529)
                     /locus_tag="ACHUPT_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR0821087.1"
                     /translation="GALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSLQTAISG
                     GTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT         1670 a         1618 c         1451 g         1790 t
ORIGIN      
        1 aataaaacct taaaaatatt ctttaaaatc tatgtttcag tcatccttat tgcaatgata
       61 aaattaaact tttttataac ccctcttcct gacatgaact ggtctcaagg agcagaatta
      121 ataaataaag caaaacatgg agaagccgtc tcatttacag tgctccctcc atggttaact
      181 cttgatttaa taaaaaaata aaacttcaca ttaataaata acaatgaatc cgcctcccta
      241 cgtattcccg tagggaggct gtcgtgttta tagtagcctg gtaatgctaa atgcaaacga
      301 actagagatt cctgctccgc taagcgtatt gcgcagggtc accctaaatg tatctacgcc
      361 aatataagta acatcacaac taactgaatc aggcgcagat ataggtgtta tctgcacaat
      421 ataaccgcta gcaactgctg cgagcggatg agaaaagcta acctcataca cacctgtagc
      481 aaccacgtta accgatgaaa caccagtagc aacgtcgata ctaccattgg cgttaatttt
      541 tcccgagcgt gtgactgaca atgcagaagc cgatgttgcc ttactggttg tttctgtaaa
      601 aacacaccca atgtcaacta cctcgcaatt agctcctgtt atccagtatg gtcgatctgc
      661 acttggctga taatcccctg cgctctggaa cgttgtgccg ttgagtatga ctgtaacttt
      721 tcccccacct gagcttgtta cttgaatatt agtgtgcgtg tatcttgcac tgctcacgcg
      781 atgaaaattt ccaccgttta taaccacagt tacagggcgc gtcccagtat tatctatagc
      841 aaggtctgcc cccccggcat tagcctcaaa gtatgggttg tttaatacca aagcgcacga
      901 accgttaagg ccatttatgg ctaattgcgc accacctgta ctggaatccc cctgagtacc
      961 atttccttca caagtcaggc tattgatagt tgtaccagca ccccagcggt ccccgaggat
     1021 accccattca ctgttctgac tgcacgtaag cctatttgca gttatagcgt tcagttcaga
     1081 atgatttacg gagttggtag cacgccaacc gatacgatta ctctgcaagt acacgttcgt
     1141 catgtcgctc gtgagcatcg agtcagtgcg taacccttcc ccgtcagggt ggcggcagat
     1201 aacaatgttt tcaagacgag tatgtgcttt attctgcacc agcataccgt aacttccggg
     1261 attccctttc agcgtcatgt tgccgatgta gtcatatgca gccaatctct gactgttaaa
     1321 cgctgcgtca cctagcattt ttacgttaaa atcagtagct ctacattcca gtattgtctc
     1381 agccatgttc tcgcctgagt aaaaacagcg gacacccgga gcacctacaa agtcggcagg
     1441 gtaatcatca taattaagta ccagaggagt ggtaacatca tacacccctt gctttatttt
     1501 taatgttcca ccgaaaggcc acgatgtaag cgggtcttga tatatttcat tttttattgc
     1561 aaatagctca tcttgaagat ttttcccgct attagtgcca atgtaagtag ccccttcatc
     1621 agattgaagt aactgcttta actgatccgg gtcatacttc agcaaattcg gaaaatagaa
     1681 ctgttgcgaa ccatacgcat catatacagc cattgaatgg ccttgctcag ttacaaattt
     1741 ggcaatctgc ccgttgtaca caggatatcc ggcagcattg ataacaattg gctgagaaac
     1801 agggacgtga gatccgtctt cattctctac ataaacctga atctggtttt caggatttac
     1861 cgggtcagtg tcaattttcc cgatataaat ttttccattg gcatttgctt taaacgagcg
     1921 agctaaagta aataactgac ttggttgtga aacaacaacg ttgtataatt gttctggcat
     1981 aataaacatt ccggagaaaa taatgagtaa gagacgtcac gaggtagtaa ctcgggagcg
     2041 ccttatggag gttctctatt acaataaaga aacaggaatt ttcacttgga aaaagaaatt
     2101 aagcgccaga ggtgctgttg ggaaagttgc tggcacaatt tcttatggat ataacgcaat
     2161 taatattgat ggcgtaagat attttgcaca tagacttgcc tggctgtatg tatatggcga
     2221 atggcccaaa caagagattg accacattga tagaaacaga agaaataacg caatctcaaa
     2281 ccttagggat gtcagcagag tcgtaaatgc attaaatgtc ggacctcgaa atagcaatgc
     2341 tggaatcaag ggggtaactt tctgtcaagc cagaaatcaa tggcaagctc agataaatgt
     2401 ctcaggtaaa aacataaccc ttgggagatt caacaccatt gatgaagcgg ctatagctta
     2461 taaggcagca aacatggttg ctgaccatct gttgagtagg tgatatctga catttcattg
     2521 ctccagacga atgatatgat gcaaccatga tgtgattgca taccgaaatg gtactattga
     2581 gtatttatcc agtaagttac gatgccattc cacccaattg gtgaggcatc aaggatgtac
     2641 agcaaatacg acgaggcgca gtttcacttg agacttccgc atgaactcca cgcgaaaatt
     2701 aagcagcgtg cgaagatgaa taacaggtct ctgaactcag agataattgc agcgattgaa
     2761 gaatcactgg ataaacaaaa ctctgcatca gtttacattg acgatgcaga gcgtatggca
     2821 gaacaacaat ccgatatggt taagaaaatt gtctttgata cgctcaaaga gctatataaa
     2881 aaagacagca gctaaccatc agttacggag gatttatgca aagagatatg ctgaatattg
     2941 cgttctacat atttggtttt tgcacgttcc tggtgtttgc gaagctattc tgacaacgca
     3001 tcagacttgg caccctgagt cagggcgtta atggcctttt gcgcctgctg catggctttc
     3061 tcaaacgctg ttgatccgcg tggggtattt gccattcgga gcattgcatt tctgaatggc
     3121 tcgctctcat aggcgcgagt aagaagtccg tagcttaccg ctgcgccagt tgtcgccggg
     3181 ttcattgccg tcccataccc gataatgaac gggatggttt gctgccctgt tggtgttgtt
     3241 actgccgctt ttgctgcctg ctgagtggac tgaaggtaat ttttcaatcc tttcagataa
     3301 gcagcgtcct gccccttaaa tgtgatgcca gtctggtttt gcaggatgtt aagctgccga
     3361 aggaactggt caggggatcc gccagatttc tccatcgcct ttccaatgat gccattgcgc
     3421 atttgcaccc tgccaacacg accaactgag ttatacagcg tcttaatttc cgatttgttc
     3481 ttgctgaata gcatgttgtt gacaacttcc ggcgtcaggt cgcctttcat gagaacattc
     3541 ttcagcctgg tattctttag tttcgccgct tcgtcagcgt agacggcatt ggcctgctga
     3601 tatttacgga gagtatcgtt gccaagattc tgaccaatgg caccattgat atcgtcggtc
     3661 atcgccttgt aaacgcgctg aatggcagca tcggaacggt ttggtaacac tggtcgctca
     3721 cccttcacgt ccattctgaa ctgactgcgc aggtcgctta attgcttcaa atccagattt
     3781 accggacctt cagggccagc attgcgaaca agctcatcac gatatgactg aagttttgaa
     3841 attgtctcgt tatcagccac cttaccaagc ttctgcaggt tagatatttc tgtatcaatc
     3901 tgctgaattg ctcgcgcagg ctgaatgttt actccagcca tagcattctg aacctgctcc
     3961 agtcgattac cggcggcacg acgaattcct gatgttttcg ctttaaggct gtcaataaca
     4021 accgctggat catactcccc gaatttatca gcaaatctct gcaccaactg gcttctcgct
     4081 tcctgttgcg ttgctctcat tccgcttgtg ccagccagag ggatattttc tgctgtagtc
     4141 tgcgccattt ttccgacgcg ggaagttggt tgtaacaggt ctgtggtgtg cagaggaact
     4201 ccttcacgct ctgcaaatct gatagcctgc tgagcttctg gcgcgatagc accacgaacg
     4261 ccacgataag cagcacctaa tccacgtccg gcagcgttaa tagcaccgcc agcaagtaca
     4321 ccaacgccta aatcggtggc gagtgcttcc gcatcatctt tcgcactatt tgcagcaagt
     4381 gatccaactg cgttttcagc gagaaggcga gttgcaccct gagcaattcg accagcaagt
     4441 gttggtgcct gtgttgccgc tctctcaacg ccggcaggag tgaggtaagg caatgcttca
     4501 gcaaataccc tgccctctgt cgtttgtgga gtcagcgcac cttgctgaag gccaaagtcc
     4561 tgctctaatc catgcgttgt tactcgtggc gctggttgat atgttccatc gccaatgccg
     4621 agtttaccgc cagcccatgc agccgcgctt gttacagcat cggcaaccga tgcaggtatg
     4681 tttgccacgt tcacgccagc ctgcaccagt ccgcgaccag tctcttttac tgcttcgcca
     4741 agatcagaca taaatccact ttgctgtggt tgttgctgtg ctactggttg ctgtgtctcc
     4801 actggctgca cagatggcaa tggataggca gcatagaaag cttgcttagc ctgctctgca
     4861 ttttctccgg cttgcggggc aacgacttca ttgaagtatt gctcctgagc ctgcgctttt
     4921 tgttctggtg ctaacgcctg atactgtgga gatgcgataa catctttcca tgctttagcc
     4981 attaatcacc ccatagtgaa gaaaaattac tgccagcagg agattgttgc cctggcgtat
     5041 tctgcaccgg ctcctggtaa tcaaactgtt ttttaacagt gctcaacttg ctttcaagct
     5101 gatttcgaat ctttccgata gagtcacgaa aagccttttc actcattttg ggacttagag
     5161 caccaaccgc atcggataat tttttaccct cagcatctga aagagccccc atccccttca
     5221 gggactgcac catagggagg aaagtttgag ctttaaaggt gtcgagtctt gcttcaaagt
     5281 tagccgcatc agatccagga actgtcggaa acgctgagcg aataccaact gcttttgaaa
     5341 gtccagggct ttgctctatc tcgttgagag aatcaagcgc agtgctgaac gtatcaacag
     5401 cgccctgagc ggcggcctgt ctgtcagcac gagctatttc agccttttgc cgaacatctg
     5461 cctgtttctg ctttagctct tcaagcttta actgattgct ttctctggct atctgtctgt
     5521 ccagagcctt ttcttgtaat tctgctcttt gtatttcgcg ggaaagagca gcattctgtg
     5581 cgctgatgtt ctggccacgt atctggatgt cctgacctcg tgctgttagt gcctcgccag
     5641 cctgattgct gcggattgtc tctgccagcc tacctcggtc aatctcacga ccagccatct
     5701 tgtcctgaac attgaagtaa tcaatcggac caagagcagc catcccaagg tgatcaacaa
     5761 actcaccaaa tcctgaagga ttctgctgat acatctgagc aacgttgtta ggatcaacgc
     5821 cgacgcgagt cagttccttg gcgttgtttt gcagccatga ttgcattgct tctggagacg
     5881 atgacgcaag gcgtgcgcca gccgctaagg tgccgataga attacgctgc tcttcatcaa
     5941 tgaatcccat gcctttacga acggattcaa tctggtctgg atattgagta gccaactgac
     6001 gcaaagcacc gcgatcacca gacgcataag cattagcgta cgcctgctga aattctttct
     6061 gccgctgagc ctgcttttcc tgctgaaaaa cacccgcaat acctgaaaga ccttgcaaag
     6121 cagtcagccc aacattgtta gcgcctgaac gctcaatatc attgttctgc ctgataagct
     6181 gaagcgtatt gccgatgtca tttacgctcg gagcgtttga gttgacgccg ccgataccag
     6241 ccaacaatcc gccgtttgtt ccttgccaag tagccatgat tcccccttaa aacaacgagc
     6301 caagcaatcc gataccagca ccaataccag cgccccaagg cgttgatgtt cccaaaaggc
     6361 tggcaagacc tgcaccggca atcgcaccag acgtgccacc gctaattgca gtctgaagac
     6421 ttgatggttt attggcatta gcagcggcaa gagctgcgct ttgctgtgca atgctgctca
     6481 tgttgttggc gtacgtctgc ccggcgtttg cctgaccttg cagcgcacc
//
LOCUS       DAZOZX010000175         6523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZOZX010000175 DAZOZX010000000
VERSION     DAZOZX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.178"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..117
                     /locus_tag="ACHUPT_003692"
     CDS             <1..117
                     /locus_tag="ACHUPT_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821088.1"
                     /translation="TLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            441..1829
                     /gene="narU"
                     /locus_tag="ACHUPT_003693"
     CDS             441..1829
                     /gene="narU"
                     /locus_tag="ACHUPT_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0821089.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1911..5651
                     /gene="narZ"
                     /locus_tag="ACHUPT_003694"
                     /pseudo
     CDS             1911..5651
                     /gene="narZ"
                     /locus_tag="ACHUPT_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5648..>6523
                     /gene="narH"
                     /locus_tag="ACHUPT_003695"
     CDS             5648..>6523
                     /gene="narH"
                     /locus_tag="ACHUPT_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0821090.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1559 a         1715 c         1690 g         1559 t
ORIGIN      
        1 accttagatg aaataaacaa tacttactat aataacgatt attacaatca ggcaataaga
       61 gaggaaccga tagactttct ggacagatcg ttttcctcca attcatggcc tttttagtga
      121 ataaatcatc actaattcta taagtgtctg gtcttgttga gaatatagtt aatatgtaac
      181 cctttagatt cacgatctga agggttgaaa catgacatac gttcaaagat taacccccgt
      241 ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta atccccattc
      301 tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc
      361 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat
      421 gtgtgcatat gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
      481 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa
      541 gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta
      601 gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt
      661 tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg
      721 tgcctatatt cggtggacgc cgatggccgg tttttagtac tgcaatcctg attattcctt
      781 gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta
      841 tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt
      901 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
      961 taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct
     1021 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     1081 catggatttg ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     1141 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     1201 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     1261 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     1321 ttggcccatt tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg
     1381 gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt
     1441 tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg
     1501 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     1561 ttcgccagat aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga
     1621 gagaagccgt caccgaaacg gcggcaacgc tgggctttat ctcagccatt ggcgcagtgg
     1681 gcggcttttt tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg
     1741 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt
     1801 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca accatgtcgc
     1861 atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac
     1921 ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac
     1981 aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca
     2041 aaatcgtgcg ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg
     2101 ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg
     2161 acctgcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt
     2221 acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg
     2281 aagccctcaa acaacacagc gatccggtac tggcgtgggc atcgattatg aatgatccgc
     2341 aaaagtgcct gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc
     2401 aggaactaaa ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg
     2461 atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa
     2521 cgcgttatct gtcgctgctt ggcggcacct gttaaagttt ctacgactgg tattgcgacc
     2581 tgccgcccgc ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact
     2641 ggtataactc cagctacatc atcgcttggg ggtctaacgt accgcagaca cgtacgccgg
     2701 acgcccactt ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg
     2761 actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata
     2821 gcgccctggc gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca
     2881 gcgactactt tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg
     2941 agcctcgcga cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg
     3001 atggactggg cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg
     3061 aattggtagt gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc
     3121 tggaatccat tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg
     3181 acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca
     3241 gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg
     3301 atggcaacac ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg
     3361 atcgcgggct ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc
     3421 cagcctgggg tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg
     3481 aatttgccga taccgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg
     3541 ttaaccactg gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct
     3601 gcggttgtgt cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc
     3661 gcccacaaac cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc
     3721 agatgaacag cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct
     3781 ctgctcagga gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg
     3841 atttcaacgt tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta
     3901 acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg
     3961 cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca
     4021 atcatccgcg taatttgttt gtctggcgct ctaacctgct tgactcctcc ggcaaaggcc
     4081 acgagtatat acagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg
     4141 gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca
     4201 agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata
     4261 tcgttctgcc caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc
     4321 catttattca tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg
     4381 aaatctacaa aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag
     4441 aaaccgacgt ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt
     4501 gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga
     4561 atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc
     4621 cattgatgga caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa
     4681 tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc
     4741 tgattgacac cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg
     4801 gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc
     4861 atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc
     4921 gtaaaattat ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg
     4981 cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc
     5041 tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc
     5101 ctatcgacac ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa
     5161 aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa
     5221 acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg
     5281 cccgagaact gaccattgtc gataacgact gggtggaagt atttaacgcc aacggcgcgc
     5341 tgactgcccg cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg
     5401 cccaggaacg cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc
     5461 ataactcggt cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc
     5521 tggcctgggg ctttaactac tacggcaccg tcggttcgaa ccgcgacgaa ttcatcatga
     5581 tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg
     5641 cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt aacctcgata aatgtatcgg
     5701 ctgccatacc tgttcggtga cctgtaacaa cgtctggacc ggacgcgaag gcatggagta
     5761 cgcatggttt aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga
     5821 tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct
     5881 gggcagcaag atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagcagat
     5941 tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagca caccggaagg
     6001 caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga tggacaaagt
     6061 gatctggggc ccaaactggg aagaactgct gggcggcgag tttgaaaaac gtgcccgcga
     6121 ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca ccttcatgat
     6181 gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga cctgcccaag
     6241 cggcgctatc tacaaacgcg aagaagacgg cattgtgctg attgatcagg ataaatgccg
     6301 tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca actggaaaag
     6361 cggcaagtca gaaaaatgta tcttctgtta tccgcgaatt gagtcaggcc aacccaccgt
     6421 gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt acgacgccga
     6481 ccgcatcgag gaagcgtcga gcaccgagcg cgaagttgac ctc
//
LOCUS       DAZOZX010000176         6480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZOZX010000176 DAZOZX010000000
VERSION     DAZOZX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.179"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            158..505
                     /locus_tag="ACHUPT_003696"
                     /pseudo
     CDS             158..505
                     /locus_tag="ACHUPT_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            558..953
                     /locus_tag="ACHUPT_003697"
     CDS             558..953
                     /locus_tag="ACHUPT_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0821091.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            994..1737
                     /gene="cmoA"
                     /locus_tag="ACHUPT_003698"
     CDS             994..1737
                     /gene="cmoA"
                     /locus_tag="ACHUPT_003698"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0821092.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1734..2705
                     /gene="cmoB"
                     /locus_tag="ACHUPT_003699"
     CDS             1734..2705
                     /gene="cmoB"
                     /locus_tag="ACHUPT_003699"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0821093.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="ACHUPT_003700"
     CDS             complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="ACHUPT_003700"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0821094.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5324..6424)
                     /locus_tag="ACHUPT_003701"
     CDS             complement(5324..6424)
                     /locus_tag="ACHUPT_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0821095.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1475 a         1700 c         1543 g         1762 t
ORIGIN      
        1 ataatgggct tttaatccta tcgtttattg cttgcatagt agaatctaac catccatcac
       61 tcatagctaa ccctcggaaa tcttacattt ctgtctatca taaccactag catcaccttt
      121 aggaaccatc tgaaacggga ggtgtattta gaccttgcta atcttagaca gccgcccggt
      181 tcatgcatca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
      241 tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga
      301 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
      361 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
      421 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
      481 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
      541 ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa
      601 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg
      661 gtggttttag cgaactgcaa agcgctattt gcattcatgg taacgcggtg gaatatattc
      721 ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
      781 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
      841 gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
      901 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
      961 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     1021 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtcgctga agtcttcccg
     1081 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     1141 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     1201 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     1261 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     1321 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     1381 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa
     1441 atttatcaag gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     1501 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     1561 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     1621 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg
     1681 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     1741 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     1801 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt
     1861 ccaacacggt ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg
     1921 taaccgccga aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta
     1981 tgcggaacct gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     2041 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
     2101 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
     2161 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     2221 cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac
     2281 agcttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc
     2341 gttcgccgct ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg
     2401 tgctggaaac gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt
     2461 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     2521 agaagtgtgg ttttgtagat atccgcattg tggatgtgtg cgttaccact actgaagagc
     2581 agcgacgcac tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc
     2641 cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
     2701 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc
     2761 ttgctaattc aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag
     2821 ttttacgttt gcataacctc agcgcccgtt tccgggcgct attcacttct tacgcctgaa
     2881 cagcaggctt atcaaatgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca
     2941 ccagcgcaga gttaccggca caggcatttg cccgctgcga gctggggata tccgccgtta
     3001 acacgttcgc actgccgttt ttacacaagc cgttttccag atctggccat gcaccttcat
     3061 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac
     3121 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaatcga gcagcatctt
     3181 cggtgtgaat agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
     3241 agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct
     3301 cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     3361 tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca
     3421 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct
     3481 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag
     3541 tgacgcgttg cgcacgggcc cctttctgag cggcatcata gaaaaatttc agccacgcca
     3601 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat
     3661 cggcaaacac gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg
     3721 gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg
     3781 tggtgatcgg taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa
     3841 cgacgatcat ctccggtttc tgccaggctt tgatcagacg attagtatcc tggtgatggg
     3901 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac
     3961 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     4021 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     4081 tttccggcag cacgccgcca acacgcgtcg ggttgccacc attggagtaa tgataagaga
     4141 aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     4201 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     4261 tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     4321 tttccgccgt ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     4381 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     4441 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga
     4501 tccaggtggc attatcgcca aagaattcga tggtttcgga gcggatagga tcaatggcaa
     4561 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
     4621 tgctccaggc aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc
     4681 tgttttccag aatcagcggc caggaggcct gctgttcata cacctctaca gaaccgacca
     4741 catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac
     4801 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
     4861 agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct
     4921 catgttgctc atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt
     4981 cgccacgacc tttcgccggt tgcagtggat tgtcgagata gctttttctc accatcggat
     5041 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
     5101 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgctca
     5161 tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga
     5221 gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt
     5281 cacgtcttgt taatgccata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
     5341 tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cggttcgttt tcgctcatgc
     5401 tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg
     5461 cggcaataga ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
     5521 atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac
     5581 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac
     5641 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
     5701 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
     5761 cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga
     5821 aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc agctcatgcg
     5881 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga
     5941 tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg
     6001 aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt
     6061 ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac
     6121 tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa
     6181 ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt
     6241 tctggttcga aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc
     6301 aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc
     6361 cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc
     6421 gcatagattc ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa
//
LOCUS       DAZOZX010000177         6374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZOZX010000177 DAZOZX010000000
VERSION     DAZOZX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.180"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHUPT_003702"
     CDS             <1..378
                     /locus_tag="ACHUPT_003702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821096.1"
                     /translation="IIRWLLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUPT_003703"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUPT_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0821097.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUPT_003704"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUPT_003704"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0821098.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHUPT_003705"
     CDS             complement(1787..2476)
                     /locus_tag="ACHUPT_003705"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0821099.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUPT_003706"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUPT_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0821100.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUPT_003707"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUPT_003707"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0821101.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUPT_003708"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUPT_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0821102.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1621 a         1716 c         1577 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DAZOZX010000178         6326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZOZX010000178 DAZOZX010000000
VERSION     DAZOZX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.181"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            46..399
                     /locus_tag="ACHUPT_003709"
     CDS             46..399
                     /locus_tag="ACHUPT_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0821103.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(436..2514)
                     /gene="flhA"
                     /locus_tag="ACHUPT_003710"
     CDS             complement(436..2514)
                     /gene="flhA"
                     /locus_tag="ACHUPT_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR0821104.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2588..3598)
                     /gene="hypE"
                     /locus_tag="ACHUPT_003711"
     CDS             complement(2588..3598)
                     /gene="hypE"
                     /locus_tag="ACHUPT_003711"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0821105.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3595..4716)
                     /gene="hypD"
                     /locus_tag="ACHUPT_003712"
     CDS             complement(3595..4716)
                     /gene="hypD"
                     /locus_tag="ACHUPT_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0821106.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4716..4988)
                     /gene="hypC"
                     /locus_tag="ACHUPT_003713"
     CDS             complement(4716..4988)
                     /gene="hypC"
                     /locus_tag="ACHUPT_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0821107.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4979..5851)
                     /gene="hypB"
                     /locus_tag="ACHUPT_003714"
     CDS             complement(4979..5851)
                     /gene="hypB"
                     /locus_tag="ACHUPT_003714"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0821108.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5855..6205)
                     /gene="hypA"
                     /locus_tag="ACHUPT_003715"
     CDS             complement(5855..6205)
                     /gene="hypA"
                     /locus_tag="ACHUPT_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0821109.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
BASE COUNT         1439 a         1736 c         1677 g         1474 t
ORIGIN      
        1 agattaagat gtaatataaa tgcatcttaa tgaggtggag gcagcatgtc cggcaagcgt
       61 atctctcgtg aaaaactgac gattaaaaaa atgatcgatc tttatcaagc gaaatgcccg
      121 caggcgtcag cggagccgga gcattacgag gcgttgtttg tttacgcgca aaagcggctg
      181 gataaatgtg tgttcggcga agagaaacca gcctgtaagc agtgtccggt gcactgttac
      241 cagcctgcaa agcgtgaaga gatgaaacag attatgcgtt gggccgggcc gagaatgctg
      301 tggcgtcatc cgatcttaac cgtgcgtcat ctcatcgatg acaagcgtcc ggtgccagaa
      361 ctgccggaaa aatatcgtcc gaaaaaacct cgtgaatagt atgccggaca ggcagatctg
      421 tccggcaatt tgcagttaaa tcaatgccga tttatcaatt cccagccgct tcatccgtga
      481 cagcagggtc gtgcgtttca gccccagacg ttgcgcagcg cctttaggcc cggcaaccac
      541 gctgttggtt tctttcagca cgcgcacaat caactgatat tcatcttcgc cctcctgggc
      601 gacaaccgtt gcggcaggcg gcgtttcagg ctccggtaaa gcaatatctg gcaatgacag
      661 ctgcagcacg ttgccgcgtg ttagcaatac tgcgcgctca atgacgtttt ccagttcgcg
      721 tacgttaccc ggccactcca tgttgctcaa ggtgcgcagc gtctcggcag gaatgctgtc
      781 gatattgcgc cccagacgac gggcaatttt gaaggtaaag gctttcgcca gcagcggaat
      841 atcttccgga cgctcgcgta gtggcggcag gtgaatcggg aatacgttca ggcggtaata
      901 gagatcgcta cggaactctc ggtcggcgac catttttttc agatcgcggt tagtcgcggc
      961 aattaaacgt acgtccgtct gaatgatttt gttgctgccg agacgttcaa actcctgttc
     1021 ctgcaataca cgcagcaact tcggctgtaa ctccagtggc atatcgccca cttcgtcgag
     1081 gaacagagag cttttatccg ccagttcaaa acgaccaatg cgctgggcgt tggcaccggt
     1141 aaaagcccca cgctcatgac caaacagatc gctttccagc aatccggcag gcatcgccgc
     1201 gcagttcatt ttgaccatgc ggcgattatt acgcccactg agattatgga tcgcacgggc
     1261 aatcagctct ttacccgagc cagtttcacc gaggatcagc acggtactgt cactttgcgc
     1321 caccatttca acttgtttaa gcacgctgta catggcttcg ctgcggccaa taatctcgcc
     1381 aaattcacta tcaacattgt tgagctgctc ggtcagggcg aggttttcat caaccagccg
     1441 ttctttcaga cgatggattt cctgataggc gagggcgtta tcgacagcga ttgccacacg
     1501 ttcggcaatc tggcgcagca aattcagatt ggtagtggta aacactttct cttcgcattg
     1561 cgccagtttc agcacgccca gcatggtgtc gccagacatc agcggtaaca ggcacaaggt
     1621 ttgaatctgg ttgccccagg tgtcgaacaa catgcgttca tagggggcta aatcgtcccg
     1681 ctcgtagaga ttgatcagca gcatctcttt acttttgaac acgcgttcgg tgagggttcc
     1741 ggcttcatcg acttcgctct gttcgtgggc gggatgttgt ttatcaagat agtgagtgga
     1801 gtagatgttg agtttgtttt tacggtggct gcgtaagaca atgctgatat cgtcaatgtc
     1861 gaaatagtaa tggatttctt tggcgacttc gctgaccagt tcgtccatat ccaggcggga
     1921 aagcaccgcg ttggtaatgg caaccaggat gcggaagtta tcgcgctccc ggcataacaa
     1981 ctcatagtcg acattgttga cgacgcggct ctggatttgt tcggtgacga cagaaacgat
     2041 ctgcgtaaat gtttgcagac gattgaactc tttttcgctc cagggacgct cgtcataacg
     2101 aataaattca cagccaccaa aaatatgccc tcccgccgcc agtggcatca ggcaatagtg
     2161 accaaatttg gggtatagcc cacctgcggc cagctgcggc caggtttcac aaaattcttc
     2221 gtaactgcaa tgcagcgtat cagggcgcga caaaatgctg cgtaccggac cgtgtgccag
     2281 aacagtttcg tcttcatatt taatgggggt gtctttttca cgcgacgcgt aataagacgc
     2341 acgttgagtc tgcgcttgcc acaacacaat agccgcgttg tcggcgagcg cagaacgctt
     2401 taccagttgc gaaagagcct cacacagcga ggccagatcg ggctgctgca atagtgtccg
     2461 agtgatgtcg aacaaccctt gctgtccgag atcactcatc ggtgtatatg acattttgtt
     2521 cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg actaactata ggagatttag
     2581 aattttatta gcatatacgc ggaagcggtt cggcgtgtgg taaatcgagg gttcgtttca
     2641 cgccatacag accggcaaga cgaacacctt tacgttccac cacttcacca atcagcgccg
     2701 cgtctttccc cagtggatgg gaatgtaacg ctgccagcac ttgctctgcc gcgttgcgtt
     2761 caacggcgat caccagtttg ccttcgttgg caaagttcag ggcgtccagt cccagcaatt
     2821 cgcaaacgcc gcgcacggca ggtttaaccg gcagcgctga ttcagaaatt tcaataccac
     2881 aaccgcaggc tgccgcgaac tcatgaacca ccgcgtttac accaccacgg gtggcatcac
     2941 gcagcgcttt cacgccggga atgtcacgca gcgtctgaat aagcggcgtc agcaccgcgc
     3001 agtcgctgac cagttcgcca tccagcccca gctgctcacg caggttaagg atagtcgccc
     3061 cgtggtcgcc gagtgtaccg ctaaccagca atacatcgcc tgcggttagc gtctgtgcgc
     3121 cccagtgaat attcgccgga attgcgccca tgccagcggt gttgataaac agtttatctg
     3181 ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat ggcaatgcct gccgtgcggg
     3241 cggtttctgc catgctggtc actacggctt tcagtgtctc catcggcaat ccttcttcga
     3301 ggataaagcc acaggagaga tagcgcggaa tagcgccact gcccgcaacg tcattcgcgg
     3361 tgccgcaaat cgccagcttg ccgatattac cgccaggaaa gaacagcggg tcaatgacgt
     3421 aactgtcggt ggagaacgcc agacggtcgc cttccgctac cagctgcgcc agatcaagac
     3481 gtgcctgatc ttcctgctct gccagccacg ggttggcaaa ggcttccata aacaggctgt
     3541 tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc gagttggata ttattcacgc
     3601 ttcactctcc tgctgacgat actgatacca cgcggcgcac gctccttcgg aggagaccat
     3661 cagtgcacca aatgcggttt gaggattaca ggtgttacca aacagcgggc attgatgcgg
     3721 cttacatttg cccgttaata cctcaccaca acgcgcgcgc gggtcatcgc agacctgctg
     3781 cggtgccggg cggaaatgtg cttcggcatc gaatcgttga taatccggcg tcaggtgcac
     3841 gccagaagat tcaatcacgc ctaagccgcg ccattcgctg tcgccgttga cacagaacac
     3901 atcggcaatc gcctgttgcg ccagtaggtt accggcatcc ggtaccaccc gacgatactg
     3961 attctctacc ttgctgtggg ccgctatttt ctgctccacc agcatgacca cgccttgcag
     4021 tagatcaagg ggttcgaaac cagcgaccac cagcggacga tgaaaatcgc tggcgataaa
     4081 attataggca tcagtgccga taaccatact aacgtggccc ggcgcgagga acgcgtcgat
     4141 accgttatcc ggctgttcca gcaaactgcg cagcgtcggg ataagcgtaa tgtgctggca
     4201 gaagaagtaa aaattctgca catcacgcgc tttcgcctgt tgcagagtga tggcggtggt
     4261 cggcatggtg gtttcaaaac ctaagccgaa gaacaccact ttgcgggttg gattctcctg
     4321 cgccagtttc aacgcatcca tcggcgagta aacgatgcgc acatcggcac cgcgtgcttt
     4381 tgcctgcaac agcgatccct gtttccctgg tacgcgcatg gcgtcgccaa aggtacagaa
     4441 gatgacttcc ggatggctgg caatctccac gcaggtgtcg attctaccca tcggcagtac
     4501 gcacaccggg caacccggac cgtggataaa ctcaacgttt tcaggcagta actggtcgag
     4561 gccgaattta aaaatggcgt gggtatgacc gccacacact tccataatcc gcagagggcg
     4621 ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga tgctcaatta actgcatcac
     4681 ctgttccggc gcgcgatatt catcaacaaa acgcattatt tttcctcgcc atacaacagc
     4741 gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg cgtcgagagt gtcgcgtgct
     4801 tcggcttcat taattacgct catggcaaag ccaacgtgaa ccagtaccca ctggcccacg
     4861 cgcggctgac cgttttcatc gcagctgccg actaacgtta aatcgacatc gcgctgaatg
     4921 ccgcagacgt cgactttcgc ctggttgccg tcaatggtgc ggatctggcc gggaacgcct
     4981 atgcacatcg ctgtgtctcc agccagttca gccactggtc catcccttcg ccgctggtgg
     5041 cggaaataag gatgatttca atttctggat tgacttcgcg ggcgcaggcg atgcacttct
     5101 caacgtcaaa gttgagatac ggcaacaggt caactttgtt gagcagcatc agcgaggcgg
     5161 cggcaaacat atgcggatat ttcagcggtt tgtcttcacc ttcggtaacg gaaagcaccg
     5221 ctactttgtg tttttcaccg agatcgaagc tggccgggca tacgaggttg ccaacgtttt
     5281 cgataaacag aataccgtta tcgtccagtg gcagacgcgg tgcggcgtcg gcaatcatct
     5341 gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat cgctggtgta ccggtagcgc
     5401 gaatgcgtgc ggcatcgttc acggtttgct ggtcgccttc aataaccgcg cacggaacgc
     5461 tgtctttcag gcgcattaag gtttccgtca gcagggtggt tttaccggaa ccagggctgg
     5521 aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg cgcgcggttg cgttcagcca
     5581 gacggttgtt tttgtccagc acgtcaattt cgacttccag catccgacgc tggctcatgc
     5641 ccggtgtgtg agtgcccgct tcgccatgac cgtaatgcag gtcgccttct tcagtctggc
     5701 tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat ctgcgggcgt gccgccgggg
     5761 caaatggcgc gctacgaaac gcggaatgag ggttatgttc atcaccctcg atatacaggt
     5821 tgccttcacc gcaaccgcat gttgtacaca tcgctcactc ctggtctatt tctatccgcc
     5881 gaatctgtaa accgtcgtct gccacaatct gtagcatgtc accatgacac tgtggacagc
     5941 ggcggacgcg ctgggtcagt agcgtcacat actgttggca tgtttcacac cagcattcgg
     6001 cctcttgttc ttcgaggtgc agtttacaac cttccgccac gctgccgcgg caaaccagat
     6061 caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa tgcgccaatt ttgagccaga
     6121 ccccagttac gcgttttgcg ccgtgttttg cggcctgctg ttcgatcaat tccagtgccc
     6181 gttggcagag ggttatttcg tgcatatcgc ctcccattaa ctattgccag ctacaagcaa
     6241 taattgcgcc agtgttgatt atccctgcgg tgaataatgt cgatgatgtc gaaatgacac
     6301 gtcgacacgg cgacgaaatt catctt
//
LOCUS       DAZOZX010000179         6267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZOZX010000179 DAZOZX010000000
VERSION     DAZOZX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.182"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUPT_003716"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUPT_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0821110.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUPT_003717"
     CDS             2219..2767
                     /locus_tag="ACHUPT_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0821111.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUPT_003718"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUPT_003718"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0821112.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUPT_003719"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUPT_003719"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0821113.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUPT_003720"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUPT_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0821114.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1568 a         1543 c         1595 g         1561 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttc
//
LOCUS       DAZOZX010000180         6260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZOZX010000180 DAZOZX010000000
VERSION     DAZOZX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.183"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHUPT_003721"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHUPT_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0821115.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHUPT_003722"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHUPT_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0821116.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHUPT_003723"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHUPT_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="ACHUPT_003724"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="ACHUPT_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1663 a         1455 c         1471 g         1671 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgttaa ttaacaaatg atatcttcta tatattttta
     6181 ttgtttatag atggctattt cggtaaaaat gcattttcag aatatcaacc agcggtaaat
     6241 cgaagtaaag ggagtgaatt
//
LOCUS       DAZOZX010000181         6168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZOZX010000181 DAZOZX010000000
VERSION     DAZOZX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.184"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..464
                     /gene="ipgC"
                     /locus_tag="ACHUPT_003725"
     CDS             <1..464
                     /gene="ipgC"
                     /locus_tag="ACHUPT_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR0821117.1"
                     /translation="LNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAYDF
                     YNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKNDY
                     TPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            470..2212
                     /gene="ipaB"
                     /locus_tag="ACHUPT_003726"
     CDS             470..2212
                     /gene="ipaB"
                     /locus_tag="ACHUPT_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR0821118.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            2175..3323
                     /gene="ipaC"
                     /locus_tag="ACHUPT_003727"
     CDS             2175..3323
                     /gene="ipaC"
                     /locus_tag="ACHUPT_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR0821119.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            3374..4372
                     /gene="ipaD"
                     /locus_tag="ACHUPT_003728"
     CDS             3374..4372
                     /gene="ipaD"
                     /locus_tag="ACHUPT_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR0821120.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            4381..>6168
                     /gene="ipaA"
                     /locus_tag="ACHUPT_003729"
     CDS             4381..>6168
                     /gene="ipaA"
                     /locus_tag="ACHUPT_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR0821121.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDI"
BASE COUNT         2287 a         1252 c         1028 g         1601 t
ORIGIN      
        1 ctttaaatat caccgaaaat gaaagcatct ctactgcagt aattgatgca attaactctg
       61 gcgctacact gaaagatatt aatgcaattc ctgatgatat gatggatgac atttattcat
      121 atgcttatga cttttacaac aaaggaagaa tagaggaagc tgaagttttc ttcaggtttt
      181 tatgtatata cgacttttac aatgtagact acattatggg actcgcagct atttatcaga
      241 taaaagaaca gttccaacaa gcagcagacc tttatgctgt cgcttttgca ttaggaaaaa
      301 atgactatac accagtattc catactggac aatgccagct tcggttgaaa gcccccttaa
      361 aagctaaaga gtgcttcgaa ctcgtaattc aacacagcaa tgatgaaaaa ttaaaaataa
      421 aagcacaatc atacttggac gcaattcagg atatcaagga gtaattatta tgcataatgt
      481 aagcaccaca accactggtt ttcctcttgc caaaatattg gcttccactg agcttggaga
      541 caatactatc caagctgcaa atgatgcagc taacaaatta ttttctctta caattgctga
      601 tcttactgct aaccaaaata ttaatacaac taatgcacac tcaacttcaa atatattaat
      661 ccctgaactt aaagcaccaa agtcattaaa tgcaagttcc caactaacgc ttttaattgg
      721 aaaccttatt caaatactcg gtgaaaaatc tttaactgca ttaacaaata aaattactgc
      781 ttggaagtcc cagcaacagg caagacagca aaaaaaccta gaattctccg ataaaattaa
      841 cactcttcta tctgaaactg aaggactaac cagagactat gaaaaacaaa ttaataaact
      901 aaaaaacgca gattctaaaa taaaagacct agaaaataaa attaaccaaa ttcaaacaag
      961 attatccgaa ctcgacccag agtcaccaga aaagaaaaaa ttaagccggg aagaaataca
     1021 actcactatc aaaaaagacg cagcagttaa agacaggaca ttgattgagc agaaaaccct
     1081 gtcaattcat agcaaactta cagataaatc aatgcaactc gaaaaagaaa tagactcttt
     1141 ttctgcattt tcaaacacag catctgctga acagctatca acccagcaga aatcattaac
     1201 cggacttgcc agtgttactc aattgatggc aacctttatt caactagttg gaaaaaataa
     1261 tgaagaatct ttaaaaaatg atctggctct attccagtct ctccaagaat caagaaaaac
     1321 tgaaatggag agaaaatctg atgagtatgc tgctgaagta cgtaaagcag aagaactcaa
     1381 cagagtaatg ggttgtgttg ggaaaatact tggggcactt ttaactatcg ttagtgttgt
     1441 tgcagcagct ttttctggag gagcctctct agcactggca gctgttggtt tagctcttat
     1501 ggttacggat gctatagtac aagcagcgac cggcaattcc ttcatggaac aagccctgaa
     1561 tccgatcatg aaagcagtca ttgaaccctt aatcaaactc ctttcagatg catttacaaa
     1621 aatgctcgaa ggcttgggcg tcgactcgaa aaaagccaaa atgattggct ctattctggg
     1681 ggcaatcgca ggcgctcttg tcctagttgc agcagtcgtt ctcgtagcca ctgttggtaa
     1741 acaggcagca gcaaaacttg cagaaaatat tggcaaaata ataggtaaaa ccctcacaga
     1801 ccttatacca aagtttctca agaatttttc ttctcaactg gacgatttaa tcactaatgc
     1861 tgttgccaga ttaaataaat ttcttggtgc agcgggtgat gaagtaatat ccaaacaaat
     1921 tatttccacc catttaaacc aagcagtttt attaggagaa agtgttaact ctgccacaca
     1981 agcgggagga agtgtcgctt ctgctgtttt ccagaacagc gcgtcgacaa atctagcaga
     2041 cctgacatta tcgaaatatc aagttgaaca actgtcaaaa tatatcagtg aagcaataga
     2101 aaaattcggc caattgcagg aagtaattgc agatctatta gcctcaatgt ccaactctca
     2161 ggctaataga actgatgttg caaaagcaat tttgcaacaa actactgctt gatacaaata
     2221 aggagaatgt tatggaaatt caaaacacaa aaccaaccca gattttatat acagatatat
     2281 ccacaaaaca aactcaaagt tcttccgaaa cacaaaaatc acaaaattat cagcagattg
     2341 cagcgcatat tccacttaat gtcggtaaaa atcccgtatt aacaaccaca ttaaatgatg
     2401 atcaactttt aaagttatca gagcaggttc agcatgattc agaaatcatt gctcgcctta
     2461 ctgacaaaaa gatgaaagat ctttcagaga tgagtcacac ccttactcca gagaacactc
     2521 tggatatttc cagtctttct tctaatgctg tttctttaat tattagtgta gccgttctac
     2581 tttctgctct ccgcactgca gaaactaaat tgggctctca attgtcattg attgcgttcg
     2641 atgctacaaa atcagctgca gagaacattg ttcggcaagg cctggcagcc ctatcatcaa
     2701 gcattactgg agcagtcaca caagtaggta taacgggtat cggtgccaaa aaaacgcatt
     2761 cagggattag cgaccaaaaa ggagccttaa gaaagaacct tgccactgct caatctcttg
     2821 aaaaagagct tgcaggttct aaattagggt taaataaaca aatagataca aatatcacct
     2881 caccacaaac taactctagc acaaaatttt taggtaaaaa taaactggcg ccagataata
     2941 tatccctgtc aactgaacat aaaacttctc ttagttctcc cgatatttct ttgcaggata
     3001 aaattgacac ccagagaaga acttacgagc tcaataccct ttctgcgcag caaaaacaaa
     3061 acattggccg tgcaacaatg gaaacatcag ccgttgctgg taatatatcc acatcaggag
     3121 ggcgttatgc atctgctctt gaagaagaag aacaactaat cagtcaggcc agcagtaaac
     3181 aagcagagga agcatcccaa gtatctaaag aagcatccca agcgacaaat caattaatac
     3241 aaaaattatt gaatataatt gacagcatca accaatcaaa gaattcgaca gccagtcaga
     3301 ttgctggtaa cattcgagct taatatatcc aagagccata ataatatatg gctcttcctg
     3361 taaggaaata accatgaata taacaactct gactaatagt atttccacct catcattcag
     3421 tccaaacaat accaacggtt catcaaccga aacagttaat tctgatataa aaacaacgac
     3481 cagttctcat cctgtaagtt cccttactat gctcaacgac acccttcata atatcagaac
     3541 aacaaatcag gcattaaaga aagagctttc acaaaaaacg ttgactaaaa catcgctaga
     3601 agaaatagca ttacattcat ctcagattag catggatgta aataaatccg ctcaactatt
     3661 agatattctt tccaggcacg aatatccaat taataaagac gcaagagaat tattacattc
     3721 agctccgaaa gaagccgagc ttgatggaga tcaaatgata tctcatagag aactgtgggc
     3781 taaaattgca aactccatca atgatattaa tgaacagtat ctgaaagtat atgaacatgc
     3841 cgttagttca tatactcaaa tgtatcaaga ttttagcgct gttctttcca gtcttgccgg
     3901 ctggatctct cccggaggta acgacggaaa ctccgtgaaa ttacaagtca actcgcttaa
     3961 aaaggcattg gaagaactca aggaaaaata taaagataaa ccgctatatc cagcaaataa
     4021 tactgttagt caggaacaag caaataaatg gcttacagaa ttaggtggaa caatcggcaa
     4081 ggtatctcaa aaaaacgggg gatatgttgt cagtataaac atgaccccaa tagacaatat
     4141 gttaaaaagc ttagataatc taggtggaaa tggcgaggtt gtgctagata atgcaaaata
     4201 tcaggcatgg aatgccggat tctctgccga agatgaaaca atgaaaaata atcttcaaac
     4261 tttagttcaa aaatacagta atgccaatag tatttttgat aatttagtaa aggttttgag
     4321 tagtacaata agctcatgta cagatacaga taaacttttt ctccatttct gaggtgcgcc
     4381 atgcataatg taaataatac tcaagcgcca acattcttat ataaggcaac ttcaccatca
     4441 tcaacagaat acagcgagtt aaaaagcaaa atatccgata tccatagttc gcaaacttct
     4501 ctaaaaacac cagcatcagt gtctgaaaaa gaaaactttg caacgtcttt taatcagaaa
     4561 tgtcttgatt ttttattttc ttcctcaggg aaagaagatg tgttaagaag catttattcc
     4621 aactcaatga atgcgtatgc caaaagcgag attctcgaat tttcaaatgt tttgtactcc
     4681 ttagtacatc aaaatggtct taattttgaa aacgaaaagg gacttcaaaa aattgtcgca
     4741 cagtattcgg aactaattat aaaagataaa ttatcccaag attctgcctt tggaccatgg
     4801 tcggcaaaga ataagaaact ccatcaatta cgacaaaaca ttgagcacag acttgcacta
     4861 ttagcacaac aacacacatc tggtgaagct ttatcattgg gacaaaaact cctcaatact
     4921 gaagtatcat catttatcaa gaataatatt cttgctgaat taaagttaag taatgaaact
     4981 gtttcatctc tcaaactaga tgatttagtt gacgcacagg caaaacttgc ctttgatagt
     5041 ttgcgcaatc aacgtaaaaa tactattgat agtaaaggat ttggtatagg taaactgtca
     5101 agagacttaa atacagtagc cgtgtttcct gagctgttga gaaaagtcct taatgatatt
     5161 ttagaagata taaaagattc gcatcctatc caagatggcc tccctacacc tcccgaagat
     5221 atgccagatg gcggaccaac ccccggagcc aatgagaaaa catcccaacc tgtaattcac
     5281 tatcatataa ataatgataa tagaacttac gataatagag tttttgacaa cagagtatat
     5341 gacaatagct atcacgagaa cccagaaaat gatgcacagt ctcctacttc tcagacaaac
     5401 gatctattat cccgtaacgg aaactcatta ctaaatccac aaagagcact agttcaaaaa
     5461 gtaacttccg ttctaccaca ctctatatca gatactgtcc agacatttgc aaataattca
     5521 gctttagaaa aggctttcaa ccatactcca gataattcgg atggaatagg ttcagacctg
     5581 ttaactacga gtagtcaaga aagatctgca aataactctc tttctcgggg acacaggcct
     5641 ctgaacatac agaactcttc aaccaccccc cctctccacc cggaaggagt gacaagcagt
     5701 aatgataact catcagatac aactaaaagt agcgcttctc tttctcatag agtagcttcg
     5761 caaatcaata aattcaactc aaacactgat tcaaaagtac ttcagactga ttttttatca
     5821 agaaatggag acacatattt aacacgggaa acgatatttg aagcttcaaa aaaagtaaca
     5881 aactccctaa gtaatcttat atctctcatt ggaactaaat caggaacaca agaacgagag
     5941 ttacaggaaa aatcaaagga cattacaaaa tccacaacag aacatagaat aaacaacaaa
     6001 ttaaaagtta cagatgcaaa tataagaaac tacgtaacag aaaccaacgc agatacaatt
     6061 gataaaaatc atgcgatcta tgaaaaggca aaagaagtat ctagcgccct cagcaaggta
     6121 ttgtcaaaaa ttgacgatac ctctgcagaa ttacttacag atgatata
//
LOCUS       DAZOZX010000182         6105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZOZX010000182 DAZOZX010000000
VERSION     DAZOZX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.185"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..84
                     /locus_tag="ACHUPT_003730"
     CDS             <1..84
                     /locus_tag="ACHUPT_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821122.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHUPT_003731"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHUPT_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="ACHUPT_003732"
     CDS             complement(1611..2201)
                     /locus_tag="ACHUPT_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0821123.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHUPT_003733"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHUPT_003733"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0821124.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="ACHUPT_003734"
     CDS             3179..3715
                     /locus_tag="ACHUPT_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0821125.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="ACHUPT_003735"
     CDS             complement(3756..4616)
                     /locus_tag="ACHUPT_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0821126.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHUPT_003736"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHUPT_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0821127.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHUPT_003737"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHUPT_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0821128.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1567 a         1544 c         1512 g         1482 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcggaaa gtgaagaaat taaca
//
LOCUS       DAZOZX010000183         6080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZOZX010000183 DAZOZX010000000
VERSION     DAZOZX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.186"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUPT_003738"
     CDS             complement(<1..143)
                     /locus_tag="ACHUPT_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR0821129.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHUPT_003739"
     CDS             379..651
                     /locus_tag="ACHUPT_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR0821130.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHUPT_003740"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHUPT_003740"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0821131.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1749
                     /locus_tag="ACHUPT_003741"
     CDS             1111..1749
                     /locus_tag="ACHUPT_003741"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0821132.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHUPT_003742"
                     /pseudo
     CDS             complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHUPT_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3132..3785)
                     /locus_tag="ACHUPT_003743"
     CDS             complement(3132..3785)
                     /locus_tag="ACHUPT_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0821133.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3785..5256)
                     /locus_tag="ACHUPT_003744"
                     /pseudo
     CDS             complement(3785..5256)
                     /locus_tag="ACHUPT_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5229..6080)
                     /locus_tag="ACHUPT_003745"
                     /pseudo
     CDS             complement(5229..6080)
                     /locus_tag="ACHUPT_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1428 a         1623 c         1609 g         1420 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggttt gcgtggtttg cgtggatctt cggtgcgttg
     1681 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     1741 cgtcagtaat tgcaatgtta tttgctactg cccgggccgc gttcaccggt tgcaaccgga
     1801 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     1861 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     1921 gaaacttgct gatctggttt gatattccat gctttccaca tagcggtaat atcatcggcg
     1981 ctacgcatgg tgccatccgg gttatagaaa tcttccatat gcgtcgagtc gctaccagcg
     2041 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     2101 gtaccgataa attctggcca cgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     2161 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     2221 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     2281 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     2341 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     2401 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     2461 agcggttcgc tttccacttc gttggtatcg atgtagtcag cgccaggaat atggctgata
     2521 aggtaaagct taggagcgcc ctaggccgct tcaatgactt tccagtcacc gacaggtttc
     2581 gccgtaacct ctttaccttg ttgcaggtca tgcagccatt gcggataaac cagctgctca
     2641 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
     2701 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt caacatcttt gccattaccg
     2761 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     2821 tcggtgctca ttttgtccag ccagctagca gagaggttta aggcggcagg ttcatgacca
     2881 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     2941 gctttgccat tttgttgctg aagttggtca agtgtaagag gcttcgccag ttcagcggcc
     3001 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     3061 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     3121 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     3181 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagg
     3241 gcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     3301 ctgaatggct catccaggag taacgctttt ggctgggcga gaagggcgcg tagtagagca
     3361 acgcgcgctc gctgaccgcc agacaaagtg gcaggatcct gatggaaagc tccgtcaagg
     3421 cctgaacgct caagtgcatc attcacggca ttacgtcggg cattcccctt aagtgtcgcc
     3481 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctga
     3541 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     3601 catagctcac ctgtacaaga aaactgtccg gccagtgcac caatcatcca tgaaaacaga
     3661 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     3721 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     3781 agcattagcg gattccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     3841 caaaaataat aagcggtaac aacagttgcc ataacgcctg ggcggcgaga ataccgttgc
     3901 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     3961 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     4021 gcacagggcg caacatgagt gggcatttca cgtaaaagaa gattttggcc cgcgaccagc
     4081 ccagtgtttg cgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     4141 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     4201 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     4261 atagaatggg caaccatagc cacaactggc gacgctgtgc cccccattcc agccacagca
     4321 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     4381 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     4441 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     4501 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccaaaacgta ggcggcgagt
     4561 aacagcatca gcagcagact ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga
     4621 gttaaccact gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc
     4681 gccacatcca cgaccgaaag cgaccaggca acaatcgcca gcatcgccat tgccagcggc
     4741 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg ctcaatactg
     4801 ccactaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     4861 tccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     4921 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     4981 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     5041 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     5101 ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt gtcaacgaca acgcaggcga
     5161 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     5221 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     5281 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     5341 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacaacg
     5401 ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac taccttcgcc
     5461 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     5521 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     5581 gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     5641 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     5701 tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     5761 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     5821 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     5881 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     5941 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc tccccgcacc
     6001 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     6061 aaattggcct cttttaaggt
//
LOCUS       DAZOZX010000184         5889 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZOZX010000184 DAZOZX010000000
VERSION     DAZOZX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.187"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHUPT_003746"
     CDS             <1..244
                     /locus_tag="ACHUPT_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821134.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHUPT_003747"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHUPT_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUPT_003748"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUPT_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0821135.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUPT_003749"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUPT_003749"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0821136.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUPT_003750"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUPT_003750"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0821137.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUPT_003751"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUPT_003751"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0821138.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="ACHUPT_003752"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="ACHUPT_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1399 a         1454 c         1598 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaa
//
LOCUS       DAZOZX010000185         5843 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZOZX010000185 DAZOZX010000000
VERSION     DAZOZX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.188"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            221..1129
                     /locus_tag="ACHUPT_003753"
     CDS             221..1129
                     /locus_tag="ACHUPT_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR0821139.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1175..3166)
                     /gene="tkt"
                     /locus_tag="ACHUPT_003754"
     CDS             complement(1175..3166)
                     /gene="tkt"
                     /locus_tag="ACHUPT_003754"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0821140.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3444..4202
                     /gene="loiP"
                     /locus_tag="ACHUPT_003755"
                     /gene_synonym="yggG"
     CDS             3444..4202
                     /gene="loiP"
                     /locus_tag="ACHUPT_003755"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0821141.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4408..5328)
                     /gene="speB"
                     /locus_tag="ACHUPT_003756"
     CDS             complement(4408..5328)
                     /gene="speB"
                     /locus_tag="ACHUPT_003756"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0821142.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5464..>5843)
                     /locus_tag="ACHUPT_003757"
     CDS             complement(5464..>5843)
                     /locus_tag="ACHUPT_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821143.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1406 a         1544 c         1527 g         1366 t
ORIGIN      
        1 tcaactttgt atttgttatg ttcatcacaa aatcaacatt ttgtttgtta ttgtatttgt
       61 gatgcatatc tctatttatt tattgaatgt tcgaaaacat acttttctaa tgcaatgttt
      121 tttcgctata cccctttata gattctgaat tctcacctgt cggctgtttt ctcctcatct
      181 catgtgacat attcccatac tttactcaag gaagattgtc atgaaagcac ctgtttcatt
      241 aacctcattt cgcccacaaa aatctcttgc tatcgccatt ggcgtgcttg ccgtggttgt
      301 gctgccattt ctctcgtatt acaccgttaa tgaaggtgaa cgcggcattt tgctgcgcta
      361 cggaaaaatc gtaaaagttg ccgaaccggg gctgggtttt aagatccctt ttatggaaag
      421 cgtggagaaa atttccacgc gtaatcaggc tgtggtctat cagggattac aggcctacag
      481 ccgcgaccag caaccggcac aaatgacagt atcagtcagc ttccacatta aaccttcaga
      541 agcaggcgcg gtttatacca cctacaacac tattgaagca ttaaaagatc gtctaattgt
      601 ccgtcagttg ccaactcagc tggagaacat tttcggccaa tataccgcca tcagcgccgt
      661 acaggatcgt acaaaattgg ttcaggatct gcaaaatgcc atgcgcaaag ccgttgttgg
      721 gccagtggta attgacgggg tccagattga aaatattgat ttttctgacg cttatgaaaa
      781 gtcgattgaa aaccgaatga aagcagaagt cgccattgcc acgcgtaagc aaaatctgga
      841 aacagagaaa atccaggcgc aaatagcggt aactcaggcc caggcagaag cagacagtaa
      901 actggcggca gccaaagcag aagcggaaac tattcgcgta agaggcgcgg cagaggctga
      961 aactattcgt ttaaaaagtg cggctgaagc agaagcaatc cgcctgcgcg gtgaagctct
     1021 gcgggataat cccggacttg tagccctcac cactgccgaa cgttgggacg gtaaactgcc
     1081 cgataccatg atccccggca gcacagttcc gtttatctct actaagtaaa aaaagggccg
     1141 ctttcgcggt ccttctcaca aaacgtctta ctgattacag cagcgctttt gctttcgcaa
     1201 caacgttatc aacagtgaag ccgaactctt caaacagcag ctctgccgga gcagattcac
     1261 cgaaggtggt catgccgacg atagcgccgt tcaggccaac atacttgtac cagtagtcag
     1321 caatacccgc ttctaccgca acgcgtgcag taaccgcttt cggcagtacg gattcacggt
     1381 aagccgcatc ctgcttgtca aatgcgtcgg tagacggcat ggacaccacg cgcgctttca
     1441 cgccttcggc agtcagtttt tcgtaggcag caaccgccag ttcaacttca gaaccggtgg
     1501 cgatgaagat cagttccggc tgaccagcac agtctttcag cacataacca ccgcgcgcga
     1561 tgtttgccag ctgctcttca gtacgttcct gctgcgccag gttctgacgg gagaggataa
     1621 gcgcagtcgg gccgtcctga cgctcaacgc cgtatttcca cgcgaccgcg gattcaacct
     1681 ggtcacacgg acgccatgta gacatgttcg gggtcacgcg cagagaagcg acctgctcta
     1741 ccggctggtg agtcgggcca tcttcgccca gaccgatgga gtcgtgggtg taaaccatca
     1801 cctggcgctg tttcatcagc gcagccatac gtacggcgtt acgtgcgtat tccacgaaca
     1861 tcaggaaggt ggaggtgtac ggcaggaagc caccgtgcag ggagataccg ttagcaatcg
     1921 cggtcatacc gaactcgcga acaccgtagt ggatgtagtt acctgcagca tcttcgttga
     1981 ttgctttaga accagaccac agggtcaggt tagacggtgc caggtcagca gagccgccga
     2041 ggaattcagg caacagcggg ccgaacgctt cgatagcatt ctgcgacgct ttacggctgg
     2101 cgattttcgc cggattagcc tgcagtttag cgataaactc tttcgctttg gcgtcgaagt
     2161 cagacggcat ttcgcctttc atacggcggg taaattcagc cgcttcctgc ggataagctt
     2221 tcgcgtaagc cgcaaacttc tcattccatg cagattcttt cgcctggcct gcttctttcg
     2281 catcccactg agcatagatt tcagacggga tttcgaacgg tgcatatttc cagcccagct
     2341 gttcgcgggt cagggcaatt tcagcgtcgc ccagcggcgc accgtgggag tcgtgagtac
     2401 cggctttgtt cggggaaccg aaaccgatga tggttttgca catcagcagg gacggtttgt
     2461 cagtcactgc gcgcgcttct tcaactgcgc gtttgatgga tgccgcgtca tgaccgtcga
     2521 tgtcgcgaat aacgtgccag ccgtaagctt cgaaacgcat tgctgtgtcg tcggtgaacc
     2581 agccttcaac gtgaccatcg atagaaatac cgttgtcatc gtagaacgca atcagtttac
     2641 ccagcttcag cgtacccgcc agagagcaaa cttcgtggga gatgccttcc atcatgcagc
     2701 cgtcgcccat gaaggcgtag gtgtagtggt cgacaatgtc gtggcccgga cggttaaact
     2761 gcgccgccag cgttttttct gcaatcgcca taccgactgc gttggcaata ccctgaccca
     2821 gcggaccggt ggtggtttcc acaccagcgg tataacctac ttccggatgg cccggagttt
     2881 tagagtgcag ctgacggaag tttttcagtt cttccatcgg cagatcgtaa ccggtgaggt
     2941 gcagcaggct gtagatcagc atggagccgt ggccgttgga cagcacgaag cggtcacggt
     3001 cagcccagga cggattctgc gggttgtgtt tcaggaaatc acgccacagg acttcggcaa
     3061 tgtcagccat acccataggg gcacccgggt gaccggattt ggctttctgt actgcgtcca
     3121 tgctcagcgc acgaatagca ttggcaagct ctttacgtga ggacattttg actccagatc
     3181 ggatgatgaa gggcacgccc ttaacgactt gacgacagcg cgttttgggc tacgccggaa
     3241 aatttgccaa caatttaccg caagccgcgc gtcatgtaca tggaacatcc ttttgccgct
     3301 tcagaaatct ctggatcatg ctcgcatgtt gcgcaatctg ctcgcccgtc cgctgcgctt
     3361 ttccttatac tgagactgag cgtcgattca cctgcaaacg gcgcattttt agaataatcc
     3421 tgaccttgtg cggaagagaa aacatgaaaa ttcgcgcctt attggtagca atgagcgtgg
     3481 caacggtact gaccggttgc cagaatatgg actccaacgg actgctctca tcaggagcgg
     3541 aagcttttca ggcttacagt ttgagtgatg cgcaggtgaa aaccctgagc gatcaggcat
     3601 gtcaggagat ggacagcaag gcgacgattg cgccagccaa tagcgaatac gctaaacgtc
     3661 tgacaactat tgccaatgcg ctaggcaaca atatcaacgg tcagccggtg aattacaaag
     3721 tgtatatggc gaaggatgtg aacgcctttg caatggctaa cggctgtatc cgcgtctata
     3781 gcgggctgat ggatatgatg acagataacg aagtcgaagc ggtgatcggt cacgagatgg
     3841 ggcacgtggc gttaggccat gtgaagaaag ggatgcaggt ggcgcttggt acaaatgccg
     3901 tgcgagtagc tgcggcctct gcgggcggga ttgtcggcag tttatctcaa tcacaacttg
     3961 gtgatctggg cgagaaatta gtcaattcgc aattctccca gcgccaggaa gcagaagccg
     4021 atgattattc ttacgatctt ctgcaccaac gcggcatcag cccggcaggt cttgccacca
     4081 gctttgaaaa actggcgaag ctggaagaag gtcgtcaaag ctcaatgttt gacgaccatc
     4141 ctgcatccgc cgaacgtgcc cagcatattc gcgatcgcat cagcgcggat gggattaagt
     4201 aaagcctagt ggtgtcgaac gtaggtcaga taaggcgttc acgccgcatc tgacattaat
     4261 ggcacatttg ttaccttgtg cgcattgccg gatgcgatgc tggcgcatct tatccggcct
     4321 acgggtgccg aacgtaggtc ggataaggcg ttcacgccgc atccgacatt aatggcacgt
     4381 tttacccgtg cgcatcgcat ctggtgctta ctcgcccttt ttcgccgcct gaatatacag
     4441 catctccagc gccagcgtcg ccgcagccag agcggtgatt tccgactgat cgtatgccgg
     4501 agccacttcc actacgtcca tcccaacgat gttgagatct ttcaggccgc gtaccagttt
     4561 aatggcgcga tcggaggtca ggccgccaat cactggcgta ccggttcccg gcgcgaaagc
     4621 aggatccagg cagtcgatat caaaagtcag gtaaaccggc atatcaccca caatctgttt
     4681 cacctgggcg ataacgtcat ccacgctgcg atcgttcacc tggcaggcgt ccagcacggt
     4741 aaagccgttg tctttatcaa actcggtacg aataccaatc tgcacggaat gattcgggtc
     4801 gatcagacct tctttcggcg cggtatagaa catagtgccg tggtcaaatt cacaaccgtt
     4861 cgcataggta tcggtgtggg cgtcaaagtg taccagcgcc attttgccga aatgcttcgc
     4921 atgagcacgc agcagcggca gcgtaacaaa gtggtcacca ccgaaggaga gcatacgctt
     4981 accggcagcc agcagcttct cggcgtgcgc ctgcagcttt tcgctcatct cacgggcatc
     5041 gccaaaggca tataccagat cgccgcagtc cacgacgttc agacgctcac gcatgtcgaa
     5101 attccacggg aagcggttgt gttcccaggc cagattcgtc gaaacctgac ggatcgctgc
     5161 cggaccgtgg cgaccacccg cacgaccaga agtggccata tcgaacggca cgccagtaat
     5221 cacccagtct gcatcgctgt catacggctg gaagttcatc ggcaggcgta aaaaaccaaa
     5281 ggcattggaa accagtgagt tatcgtattg atgacctaag gtgctcatgg atggactcct
     5341 attacaaagt cgatataaaa aaacccttcc gcgtcgttag gcccgacgag gaagggttgg
     5401 atttgcgaaa cacaagtttg tgctaattat cgccttaaat gggcgaggca tcaagcgagg
     5461 ttgctatttc cgacacggtg gcggcgcata gccattgtta tctgccggaa aggcttgtcc
     5521 agagcacggc ggcagcgcgt tttgcgccgg gccttgtaat tcaaaggcgg caaagccgat
     5581 taccccggta ttacgcacat cgccttgcgc cgaattggct gcgtaggaat cagcaacgtt
     5641 ggcgaactga aaagccgctt cagtatgttt atctttgcgg aatcctttaa tcactaacga
     5701 atctccggca ttcacaatat agccgttgtt attcagcgaa ccttgcttgc cgttcagcac
     5761 atccaggcca tcagccgtag caacgatctc gtagttggtg ttacgactgt aattacggac
     5821 atataattga taattcattc cga
//
LOCUS       DAZOZX010000186         5776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZOZX010000186 DAZOZX010000000
VERSION     DAZOZX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.189"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHUPT_003758"
     CDS             <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHUPT_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0821144.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
     gene            1196..2236
                     /gene="pstS"
                     /locus_tag="ACHUPT_003759"
     CDS             1196..2236
                     /gene="pstS"
                     /locus_tag="ACHUPT_003759"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0821145.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2323..3282
                     /gene="pstC"
                     /locus_tag="ACHUPT_003760"
     CDS             2323..3282
                     /gene="pstC"
                     /locus_tag="ACHUPT_003760"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0821146.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3282..4172
                     /gene="pstA"
                     /locus_tag="ACHUPT_003761"
     CDS             3282..4172
                     /gene="pstA"
                     /locus_tag="ACHUPT_003761"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0821147.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4264..5037
                     /gene="pstB"
                     /locus_tag="ACHUPT_003762"
     CDS             4264..5037
                     /gene="pstB"
                     /locus_tag="ACHUPT_003762"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0821148.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5052..>5776
                     /gene="phoU"
                     /locus_tag="ACHUPT_003763"
     CDS             5052..>5776
                     /gene="phoU"
                     /locus_tag="ACHUPT_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0821149.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1426 a         1390 c         1515 g         1445 t
ORIGIN      
        1 tcgggatatc ttattaccgc gaacgaaaat aaggttggaa cgacatttaa caccactgta
       61 actaatggat cctcatatct tggacgctgt cattgtgata ctggtaacgt aggagaattc
      121 ccctatatct attatacgtc aaaaataaac caggcattaa cttatgctgg ggttcactct
      181 aatattaatt attatgatct taatccgaac cttgatgttg ggattgctat agatattctt
      241 ggggttgggt atgtgaatgc accttttgaa tatcatgcta acaacccgtc tggtaataca
      301 aagtacaatt gcaaccgcat tgaaccttta agtatatcca gtggggctaa agcgatagta
      361 tatttttata tcaagaagac atttgcagga aaattgatta ttcctgaaac aaagatagtg
      421 acattgtatg gaacaattag ccgtgacacc ccggtggatt actcacagcc gatggctgat
      481 gtttatattc ggggcgatat tacagctccg caaagttgcg aaataaataa tttaaagcca
      541 gtttgttttg attttaaaga aataccagct gcagattttt catctgttgt tggaagtgcg
      601 gtaacaacac ataaaattac taaaacagtc actattgagt gtgaaaacct ggggatactg
      661 aataccgatg atatcagtac gtctttttat gctaccgaac ccaatacgga caattcaatg
      721 gtcgtaactt caaactctaa cgtggggata aaaatttacg acaaaaataa taaggaaatc
      781 aaagtgaacg gtggtgagtt gccaacagac atggataaat caacagtgta tggtgaaaaa
      841 tcaggtagtg taactttttc agctgctcct gcgagcctta ctggagctcg cccagccccg
      901 gggcaattta ctgcaacagc gacgataact gttgagattg tgcgctaata ggtctgacaa
      961 cgattgttca tcacatcgtt gtcatctcac ccatttcttt atcactttta aatgcatcat
     1021 gacaaaaaga tggcatgtgg aattttcatc ttcacattta aaatcagtaa gttatttggt
     1081 aattcctttc tgtcataaaa atgtcatatt ccttatatat aactgtcacc tgtttgtcct
     1141 attttgcttc tcgtagccaa caaacaatgc tttatgaatc ctcccaggag acattatgaa
     1201 agttatgcgt accaccgtcg caactgttgt cgccgcgacc ttatcgatga gtgctttctc
     1261 tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc cggtgtatgc
     1321 caaatgggct gacacttacc agaaagaaac cggtaataaa gttaactacc agggtatcgg
     1381 ttcttccggt ggcgtaaaac agattatcgc taataccgtt gattttggtg cctctgacgc
     1441 gccgctgtct gacgaaaaac tggctcagga aggtctgttc cagttcccga ccgtgattgg
     1501 cggcgtggtg ctggcggtta acattccagg gctgaagtct ggcgaactgg tgctggatgg
     1561 taaaaccctc ggcgacatct acctgggcaa aatcaagaag tgggatgatg aagccatcgc
     1621 caaactgaat ccgggtctga aactgccttc acaaaacatt gctgtagtac gccgcgcaga
     1681 tggctccggg acttccttcg tcttcaccag ctacctggcg aaagtgaacg aagagtggaa
     1741 aaacaacgtt ggtactggct ctaccgtaaa atggccgatc ggtctgggcg gtaaaggtaa
     1801 cgacggtatc gccgcgttcg ttcagcgtct gccgggtgca attggttatg ttgaatatgc
     1861 ttacgcgaag cagaacaacc tggcgtacac caaactgatc tccgctgatg gtaaaccggt
     1921 tagtccgacc gaagaaaact tcgctaatgc agcaaaaggt gcagactgga gcaaaacctt
     1981 cgctcaggat ctgaccaacc agaaaggcga agatgcatgg cctattacct ctaccacgtt
     2041 cattctgatc cacaaagatc agaagaaacc agaacaaggc acagaggtgc tgaaattctt
     2101 cgactgggcg tacaaaaccg gggctaaaca ggcgaacgac ctggattacg ccagcctgcc
     2161 ggatagtgta gttgaacagg ttcgcgctgc gtggaagacc aatattaaag acagtagcgg
     2221 taagccgctg tactaataaa actccaggcc gggtacggtg ttttacgccg catccggcat
     2281 tacaaaatga ctttgtaaac gcgtttaact gaagagtaac ttatggctgc aaccaagccc
     2341 gcttttaacc caccgggtaa aaagggcgac ataattttca gcgtgctggt aaaactggcg
     2401 gcgctgattg tgctattgat gttgggtggc attattgtct ctctgatcat ctcctcctgg
     2461 ccgagcattc agaaatttgg tctggctttc ctatggacca aagagtggga tgcaccgaac
     2521 gatatctacg gggcgctggt gccgatctac ggtacgttgg tgacttcgtt tatcgcgctg
     2581 ctgatcgccg tcccggtgag tttcggtatc gccctgttcc tgactgagct tgcgcctggc
     2641 tggctgaaac gcccgctggg tatcgccatt gagctgctgg cagccattcc aagtatcgtt
     2701 tacggcatgt ggggcctgtt tatctttgcg ccgctgttcg ccgtttactt tcaggagccg
     2761 gtcggcaata tcatgtcgaa tatcccgatt gttggcgcgc tgttctctgg ccccgcattt
     2821 ggtatcggta tcctcgcggc aggcgtgatc ctcgccatca tgattattcc gtacattgcg
     2881 gcggtaatgc gtgatgtgtt cgaacaaacc ccggtgatga tgaaagagtc ggcctacggt
     2941 attggctgca ccacctggga agttatctgg cgtatcgttc ttccgttcac caaaaatggt
     3001 gttatcggcg gcatcatgct ggggctgggc cgcgcgctcg gtgaaaccat ggcggtgacc
     3061 tttatcatcg gtaacaccta ccagctcgac agcgcctcgc tgtacatgcc gggcaatagt
     3121 atcacctctg cgctggcgaa cgaatttgcg gaagcggaat ccggtctgca cgttgccgca
     3181 ctgatggaac tgggcctgat cctgtttgtg attaccttca tcgtcctcgc cgcatcgaag
     3241 tttatgatta tgcgcctggc taagaatgag ggggcacgct aatggctatg gttgaaatgc
     3301 aaaccactgc ggcgctggct gaatctcgcc gcaaaatgca ggcgcgtcgc cgcctcaaaa
     3361 accgtattgc gctgacgctc tcgatggcga cgatggcctt cggcctgttc tggctgatct
     3421 ggattttaat gtccaccatc actcgcggta tcgacggtat gtcgctggcg ctgttcactg
     3481 aaatgacgcc gccgcccaat acggaaggtg gtggtctggc gaacgccctg gcgggtagcg
     3541 ggctgttaat tttgtgggcc acggtattcg gtacgccgct gggcattatg gcggggattt
     3601 atctggcgga atatggtcgt aaatcctggc tggcagaagt gattcgcttc attaacgaca
     3661 ttctgctctc cgcgccgtcg attgtggttg gtctgtttgt ctacaccatc gtggtggcac
     3721 agatggagca cttctccggc tgggcgggcg taattgcgct ggcgctgttg caggtgccga
     3781 tcgttatccg caccactgag aacatgctga aactggtgcc ggacagcctg cgtgaagcgg
     3841 cttatgcgct gggtacaccg aagtggaaga tgatctccgc gattacgctg aaagcgtcgg
     3901 tgtccgggat tatgaccggt atcctgctgg cgattgcccg tattgccggt gaaaccgcgc
     3961 cgctgctgtt taccgcgctc tccaaccagt tctggagcac ggacatgatg cagccgatcg
     4021 ccaacctgcc ggtgacgatc tttaagtttg cgatgagccc gtttgcggaa tggcaacaac
     4081 tggcctgggc cggggtattg atcattaccc tgtgcgtcct gctgctgaac attctggcgc
     4141 gcgttgtttt tgcgaagaat aaacacggtt aacacttttc cggatgcggc gtgaacgcct
     4201 tacccggccc tacggtaagc ctgattagcg aaaagcatca ggtacgatga ggaaaagatt
     4261 gcaatgagta tggttgaaac tgccccgagt aaaattcagg ttcgtaattt gaacttctac
     4321 tacggcaaat tccatgccct gaaaaacatc aacctggata tcgccaaaaa ccaggttacg
     4381 gcgtttatcg ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt caacaaaatg
     4441 tttgaactgt acccggagca gcgtgcggaa ggtgaaattc tgcttgatgg cgacaacatc
     4501 ctgaccaact ctcaggatat cgccctgctg cgtgcgaaag tgggcatggt gttccagaaa
     4561 ccgacgccgt tcccgatgtc catctacgac aacatcgcct ttggcgttcg tctgtttgag
     4621 aagctctccc gtgccgacat ggacgagcgc gtgcagtggg cattgaccaa agccgcattg
     4681 tggaacgaaa ccaaagataa attgcaccag agcggttact ctctctctgg tggtcagcaa
     4741 cagcgtctgt gtattgcgcg tggtatcgcc attcgcccgg aagtgctgct gctcgacgaa
     4801 ccgtgttcgg cgctcgaccc catctctacc gggcgtattg aagagctgat caccgaactg
     4861 aagcaggatt acaccgtggt gatcgtcacc cacaacatgc agcaggctgc gcgttgttcc
     4921 gaccacacgg catttatgta cctgggcgaa ttgattgagt tcagcaacac ggacgatctg
     4981 ttcaccaagc cagcgaagaa acaaacagaa gactacatca ccggtcgtta cggttgattc
     5041 aggagtgcgt tatggacagt ctcaatctta ataaacatat ttccggccag ttcaacgccg
     5101 aactggaaag tatccgcacg caggtgatga ccatgggcgg catggtggag cagcagcttt
     5161 ctgatgcaat caccgcgatg cataaccagg acagcgatct ggcgaagcgc gtgatcgaag
     5221 gcgacaagaa cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg cgcattatcg
     5281 ccaaacgtca gccgacggcg agcgacctgc gactggttat ggtgatcagt aaaaccattg
     5341 ccgagctgga gcgtattggc gacgtggcgg acaaaatctg ccgtactgcg ctggagaaat
     5401 tctcccagca gcatcagccg ttgctggtaa gtctggagtc gctgggccgt cataccatcc
     5461 agatgctgca cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa gcggtacgta
     5521 tttatcgtga agataaaaaa gtcgatcagg aatacgaagg tattgttcgt caactgatga
     5581 cctacatgat ggaagattcg cgcactattc cgagcgtact taccgcgctg ttctgtgcgc
     5641 gttcaatcga acgtatcggc gaccgctgcc agaatatttg tgagtttatc ttctactacg
     5701 tgaagggtca ggatttccgt catgtaggcg gtgatgagct ggataaactg ctggcgggaa
     5761 aagatagcga caaata
//
LOCUS       DAZOZX010000187         5744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZOZX010000187 DAZOZX010000000
VERSION     DAZOZX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.190"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..173
                     /locus_tag="ACHUPT_003764"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACHUPT_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACHUPT_003765"
     CDS             complement(489..827)
                     /locus_tag="ACHUPT_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821150.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACHUPT_003766"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACHUPT_003766"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0821151.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACHUPT_003767"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACHUPT_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACHUPT_003768"
     CDS             complement(2165..2650)
                     /locus_tag="ACHUPT_003768"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0821152.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACHUPT_003769"
     CDS             complement(2906..3886)
                     /locus_tag="ACHUPT_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR0821153.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACHUPT_003770"
     CDS             complement(3886..5085)
                     /locus_tag="ACHUPT_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0821154.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACHUPT_003771"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACHUPT_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACHUPT_003772"
     CDS             complement(5407..>5744)
                     /locus_tag="ACHUPT_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0821155.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZOZX010000188         5738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZOZX010000188 DAZOZX010000000
VERSION     DAZOZX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.191"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHUPT_003773"
     CDS             <1..241
                     /locus_tag="ACHUPT_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821156.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHUPT_003774"
     CDS             complement(238..1179)
                     /locus_tag="ACHUPT_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR0821157.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHUPT_003775"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHUPT_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHUPT_003776"
     CDS             complement(1684..2904)
                     /locus_tag="ACHUPT_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0821158.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHUPT_003777"
     CDS             complement(2908..3651)
                     /locus_tag="ACHUPT_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0821159.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHUPT_003778"
     CDS             complement(3542..5008)
                     /locus_tag="ACHUPT_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR0821160.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHUPT_003779"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHUPT_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZOZX010000189         5705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZOZX010000189 DAZOZX010000000
VERSION     DAZOZX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.192"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..411
                     /locus_tag="ACHUPT_003780"
                     /pseudo
     CDS             1..411
                     /locus_tag="ACHUPT_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACHUPT_003781"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACHUPT_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHUPT_003782"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHUPT_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR0821161.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1400 a         1430 c         1360 g         1515 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tagggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
     5701 ttatt
//
LOCUS       DAZOZX010000190         5702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZOZX010000190 DAZOZX010000000
VERSION     DAZOZX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.193"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUPT_003783"
     CDS             complement(<1..170)
                     /locus_tag="ACHUPT_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821162.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHUPT_003784"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHUPT_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0821163.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUPT_003785"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUPT_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUPT_003786"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUPT_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0821164.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUPT_003787"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUPT_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHUPT_003788"
     CDS             5484..>5702
                     /locus_tag="ACHUPT_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR0821165.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZOZX010000191         5590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZOZX010000191 DAZOZX010000000
VERSION     DAZOZX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.194"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHUPT_003789"
     CDS             complement(1..279)
                     /locus_tag="ACHUPT_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821166.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUPT_003790"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUPT_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0821167.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHUPT_003791"
     CDS             complement(1254..1670)
                     /locus_tag="ACHUPT_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821168.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHUPT_003792"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHUPT_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHUPT_003793"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHUPT_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHUPT_003794"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHUPT_003794"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUPT_003795"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUPT_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0821169.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHUPT_003796"
     tRNA            3996..4071
                     /locus_tag="ACHUPT_003796"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHUPT_003797"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHUPT_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZOZX010000192         5586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZOZX010000192 DAZOZX010000000
VERSION     DAZOZX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.195"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..92)
                     /locus_tag="ACHUPT_003798"
     CDS             complement(<1..92)
                     /locus_tag="ACHUPT_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0821170.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCT"
     gene            complement(102..877)
                     /locus_tag="ACHUPT_003799"
                     /pseudo
     CDS             complement(102..877)
                     /locus_tag="ACHUPT_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(895..1947)
                     /locus_tag="ACHUPT_003800"
     CDS             complement(895..1947)
                     /locus_tag="ACHUPT_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0821171.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(1978..2820)
                     /locus_tag="ACHUPT_003801"
     CDS             complement(1978..2820)
                     /locus_tag="ACHUPT_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0821172.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(2807..3688)
                     /locus_tag="ACHUPT_003802"
     CDS             complement(2807..3688)
                     /locus_tag="ACHUPT_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0821173.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(3709..4992)
                     /locus_tag="ACHUPT_003803"
                     /pseudo
     CDS             complement(3709..4992)
                     /locus_tag="ACHUPT_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(5006..>5586)
                     /gene="ycjM"
                     /locus_tag="ACHUPT_003804"
     CDS             complement(5006..>5586)
                     /gene="ycjM"
                     /locus_tag="ACHUPT_003804"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR0821174.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         1365 a         1508 c         1395 g         1318 t
ORIGIN      
        1 gtgcagtagt acgaagcatg aaccttatcc gccacctggc ccgcgcctat tatcgcgacc
       61 cgcagcggag agcttgtcat tgcacttttc acaaagacct cttagcaggt acgcaaccag
      121 gccaacgaat cacggtacgc ctgggcagga tcttctgccc ggatacgccc ttcatacacc
      181 acataaccct gatagttatc ggcgcgcagc tgttcaaaca gcgcgtggaa atcgagggtg
      241 ccactgcccg gctggtaacg atggttatcg gcaatatgca catgaccgag caagtcacgg
      301 ttgtcgtgca gcgcctgcgc caggttatct tcttcgatgt tcatgtgata gaaatcgccg
      361 ataatctgca catgtttaag attgttttcg acgatgtaac gacgagcatc ggcgagggtg
      421 ttgatcatat gatcctgata gcggtttaac ggttcgagat acaccacggt tccggtacgc
      481 gcggcgacct gttccagtac gcgcagggaa tcactcacca gtttgcggtc gccgtccagg
      541 ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc tgccggaacc
      601 cccccacttc tgccagcgcc tcgagaatgc gttcgatctg ctttaagcca ttaagacgac
      661 gctcttcgat aaagtcacca atccagccgt catagccgcc gcaggcggtg gtcactggta
      721 aaccggtttc tttgatcgcc gctttgactt cttcgatgtt gttgaccagc agtttgccgt
      781 caatctcaaa accatcgaag cccatctctt tgatataacg aaatctctcg cggatatttt
      841 ccggaaagaa tgcctgattc tgtgtgccga ttttcatttt ttcattcctt tgaattaaaa
      901 cgtaacgccc attttgatgc tctgttccgg atgctgatcg acatactgca tgtagctttc
      961 cgggctgttg gcaaaggtca ctaccgggtc gattaaatct tcgcaattga gataaccgtt
     1021 catcagcagt tcccagcagg tttcttcaat acgcttacgg ctccagcgcg gataatccgg
     1081 gttcggttcg ctgcacgcgc gggagaaaac aattttggcg ttattgaaat gcgcttcgcg
     1141 tccgaggtta aaaccttcag caaatggttt ggcaaacgcg acataggaga tggtgccgcc
     1201 gtaggccagg ccgcgcagcg ccgattgcag cgcgtcggcg taaccgctgg tttcgataat
     1261 cacatcagcc ccctgcttgc cggtcagcgt tttgatctct ttgcctacgt cagtgccaat
     1321 ggggttaagg cagaaatcag caccgtggcg acgggcaata tcacagcgat gggcgatagg
     1381 atcgacgcca attaccaccg atgcgccagc gcgtttcgcc agttggatgg caatttgacc
     1441 gatcgcgcca agccctacca ccacgacaaa atcccctacg cgtacgttgg catcgcgcac
     1501 gccgctcatg gcaaactgcg ccgggtcgta acagacggcg tttttccagg agctgccttg
     1561 cggcattttg cgcagcttgt agttattcac tgcgttaatg atgaccgtct cggagagcgg
     1621 gccgtagccg catacgctgt cgcccaccgc gtagtctgta acgtcgctgc cgcactcgat
     1681 aatgtcgcca accaccatgt tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc
     1741 atctgccgga cgcggggtga acatctgcca ctcgccgtta aagtcttcat cgataaacgg
     1801 gctggcggcg cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg
     1861 gatcttcact tcattggcta aaatcgcccg atcttcatac tcaaccagcg cagcaacacg
     1921 cggtgctgtg gctactaact ttttcatgta aaactccttg taaagaaact ggccgcttta
     1981 gcccttcacg ccaccggcgg tcaaaccact cttaataaaa cgttccaaaa gcgcatacat
     2041 aatcaccacc ggcaatgcgg tcaccagcga ggccgccatc atccgtcccc agatgtaatc
     2101 aggcgtactg aacagcgcgt tcaggcccac tggtaaggtg aaattgctgg cgctggagag
     2161 gaaaatcgac gcaaacagat agtcgttcca cgccaccata aagcagtaga cgaacaccga
     2221 tatcagcccg gacatcgcca ggggcacggt gatacggaag atgatttgca gacggttgag
     2281 gccatccatc atcgccgctt cttcgatctc atcagggatg gtgtcgaagt aacttttcag
     2341 cataaacacg gcggtgggca gggtttgcgt caccatggta atgatcagcg ccatctcggt
     2401 gtcgtaaata ccaagcgcgg tgatgatttt gaacagaggc accaccagca aaatgccaga
     2461 gaacatatac accgtgtaaa agctggcgtt gatggtcatc cggcctttaa agcgcaggcg
     2521 ggaaagcgcg taagctccga gaatgccgag aaataccgcc accacggagg aaaccaccga
     2581 caccaccatg ctgttacgga agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc
     2641 gacgtaatgc tccagcgtcc actgttgcgg cagcagcgta ggatgcagcg atatcgcctc
     2701 tttcgcgctt ttgaacgagg tcatcagcat cacaaaaaac ggaaacagcg tgatgatgag
     2761 aaacagcgcc agcccgcagt aaaaaccgat acgactgagc gtgcgtttat ttgttgccat
     2821 tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg aagagcacca
     2881 ccgagatcgc cgccgctttc cctaagtcat taaaggcaaa ggcggttttg tagagataga
     2941 caccaagaat atcgaccttg gttgtcagca gataaacatc agcgaacatg tagaacatcc
     3001 agatggtgcg cagggtcacc accgtcgcca ggaccggcat aattgcgggc agcgtgacga
     3061 tgcgaaaccg ctgccaggca ttagcgccat ccatctctgc cgcttcgtac agcgatttgt
     3121 cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag cgccagatgg
     3181 cgaacagcac caccagcaca aagctactgc ccggattatc aaaccacagc ggtgcctgct
     3241 catagagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg ttgttgaaca
     3301 tgtatttcca ggcaaacacc agcgaaatgg acggcgttac gtaggagagg atcaccagcg
     3361 aacgcgcggt tttgcgcagg cggaactcgc ggttgaaaaa catcgccacg gccagcccga
     3421 gcacggtgct gcccgccacc accagcgccg tataccagac cgtcatccac agcgagtgcc
     3481 agaagccggg atcggagaga atgcgcacat agttactcac cccaacaaac gttgactcga
     3541 tgttgggatt gagcggcaga cgtaaaaaac tgatttcgat attcgacacc atcggccacg
     3601 ccaccagacc gcccagcagt aataagctgg gcgcgagaag cagcagcgca aacggcatat
     3661 cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt agtgctgttc
     3721 gatcagttca tccagctttt tctggctcgc ttgcagcgtg ccagggagat cggctttacc
     3781 cacggtgacg ttatgcacca tggagctcac cacaccagaa cccgtcacat cgcccatgcg
     3841 ggtaaagttt ttatccccta ctgcgccaaa gacctgaata tttggcagtt caccgattag
     3901 ctgattcggt agttcaccca gcgccttaat aacgtcgttg tctttccagg tggcggtagt
     3961 gacaaccgct ttattcaccg gcagcgcagc acctggcgac atcatcaccc agtcggcaat
     4021 gttgtccgcc tgctccataa aggtgacaaa tttttctgct gcttcggtct tttgtccggc
     4081 ggtaatggtc agcgaggtca acatgccgta gaccgcagag tttttctcgg ttggcaccac
     4141 gaaaccgacg tttttcgggt cgccttcttt aatcacagcc ggaaggatat aggtggagta
     4201 aatcgccatc ggcgcggtgc cgttcataaa ggcgtctttc acttccatga tgtcgttaga
     4261 acccggcatg gtgttggcag caaggtcgcg gtaataggtc agcgcctgca tcatctctgg
     4321 tgtatcaagg gtgattttgc cttcggcgtt aaagacgttg ccctagttgg ataacgcaaa
     4381 ctgggagaag gattgttccg tcagcacgct ttctgccgta ggcagcgcaa taccgtattt
     4441 tttattcgcc gggtcattca gtttctgtgc aacgtccagc agctgttgcc agtttttcgg
     4501 ctcctccagc cccgcttttg ccagcacatc tttgcgatac cagatgccgc caatccaggc
     4561 gctgacagga acaccggtcc atgcgctacc atcttcggta cgcacaatac gcagtacgcc
     4621 atcgtaaaac gcgccttcac caacgttgct gatgaccgtg gcaaccgctt tgcgatcgat
     4681 aagctgctct ttatccataa ctttggcgta gtcatggctg gtttcgatca cttccggcag
     4741 cgagccgcta cgtgaaaggg tgatgacttt ggtgttatga gcgtcttctt ccacgggcac
     4801 ttgtttaacg gtgatacctg ggttttcttt ttcaaaacgg gcaatcaatt tgctgataac
     4861 tgcctgacgc tcctgctcca ccgaagaatg cataaattcg atggatacat gggttttatt
     4921 ttcttcttta cagcctgaaa tcagggcgca ggaaaccagt gctgataaca gcacgatttt
     4981 tgatttaatc atgagatgtt cctttttatt taatccacat aacctgccag gggcgcagcg
     5041 ttatttcatt acccaatata tcaacttcac taatcagatc ccgaccatgc agatcagtaa
     5101 tatttacatg ctgaatattt ttactgacat taaacaatcc agtcagacaa ttaccatcag
     5161 cgttacttct ttgaatacgc attacggatg aattaaccgt atcgatggta aaattattat
     5221 ccggatgaaa ctcgttatgg ctgcgacgaa gtgtaattaa acgcgacaac tcatgatata
     5281 ccgcatgcct taatgtagct tcatcgttca gttctcgtgt tatctcttca ctgtgatatt
     5341 ttttacggtt aatcgcacgg ttatatccga gtttttcgac acctgcgtaa tcattacgcg
     5401 agccaagaat actttgaata tatatcgcgg gtacaccggg gaaacttaac aaaatcgcat
     5461 gggcaaggat aaacctggtg caacgttctt catcgctact ctcgcggcgg cttaacgcat
     5521 tcatataggt gacattcatc tcatagggac tgcgcgtgcc gtcgggatta tttttccagt
     5581 ttacta
//
LOCUS       DAZOZX010000193         5580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZOZX010000193 DAZOZX010000000
VERSION     DAZOZX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.196"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUPT_003805"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUPT_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR0821175.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHUPT_003806"
     CDS             2739..4208
                     /locus_tag="ACHUPT_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0821176.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>5580
                     /locus_tag="ACHUPT_003807"
     CDS             4208..>5580
                     /locus_tag="ACHUPT_003807"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR0821177.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADM"
BASE COUNT         1645 a         1315 c         1328 g         1292 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
//
LOCUS       DAZOZX010000194         5558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZOZX010000194 DAZOZX010000000
VERSION     DAZOZX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.197"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..642
                     /locus_tag="ACHUPT_003808"
     CDS             <1..642
                     /locus_tag="ACHUPT_003808"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR0821178.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACHUPT_003809"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACHUPT_003809"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0821179.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="ACHUPT_003810"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="ACHUPT_003810"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0821180.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1540 g         1282 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagatat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagcat ttccttaa
//
LOCUS       DAZOZX010000195         5545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZOZX010000195 DAZOZX010000000
VERSION     DAZOZX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.198"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..694
                     /locus_tag="ACHUPT_003811"
     CDS             <1..694
                     /locus_tag="ACHUPT_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0821181.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHUPT_003812"
     CDS             complement(1033..1803)
                     /locus_tag="ACHUPT_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821182.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHUPT_003813"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHUPT_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHUPT_003814"
     CDS             complement(2294..2515)
                     /locus_tag="ACHUPT_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821183.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHUPT_003815"
     CDS             complement(2516..3199)
                     /locus_tag="ACHUPT_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0821184.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHUPT_003816"
     CDS             complement(3263..3574)
                     /locus_tag="ACHUPT_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821185.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHUPT_003817"
     CDS             complement(3571..4473)
                     /locus_tag="ACHUPT_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0821186.1"
                     /translation="MYFPSEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5545
                     /gene="parM"
                     /locus_tag="ACHUPT_003818"
                     /pseudo
     CDS             4913..5545
                     /gene="parM"
                     /locus_tag="ACHUPT_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
BASE COUNT         1407 a         1520 c         1410 g         1208 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac taggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaataca cctatgattt gggaagcact
     5101 gctgtcatag gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata
     5161 catcatgcac tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta
     5221 cctgtgaccg aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa
     5281 aaggcaaatg ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa
     5341 gttaatgtaa tgcctgaatc tttgccagct gcatttgaat cactaaaaaa agataaagta
     5401 aacaaacttg aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta
     5461 attctcggtg catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc
     5521 cgcatcactc ataccgtcat gaatg
//
LOCUS       DAZOZX010000196         5506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZOZX010000196 DAZOZX010000000
VERSION     DAZOZX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.199"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUPT_003819"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUPT_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0821187.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUPT_003820"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUPT_003820"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0821188.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHUPT_003821"
     CDS             complement(3736..4092)
                     /locus_tag="ACHUPT_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0821189.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHUPT_003822"
     CDS             complement(4096..4512)
                     /locus_tag="ACHUPT_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0821190.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUPT_003823"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUPT_003823"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0821191.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1290 a         1456 c         1444 g         1316 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttg
//
LOCUS       DAZOZX010000197         5506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZOZX010000197 DAZOZX010000000
VERSION     DAZOZX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.200"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..248)
                     /locus_tag="ACHUPT_003824"
     CDS             complement(<1..248)
                     /locus_tag="ACHUPT_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821192.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="ACHUPT_003825"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="ACHUPT_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="ACHUPT_003826"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="ACHUPT_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="ACHUPT_003827"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="ACHUPT_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHUPT_003828"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHUPT_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR0821193.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="ACHUPT_003829"
     CDS             complement(2964..3278)
                     /locus_tag="ACHUPT_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821194.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="ACHUPT_003830"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="ACHUPT_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHUPT_003831"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHUPT_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR0821195.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="ACHUPT_003832"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="ACHUPT_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="ACHUPT_003833"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="ACHUPT_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1484 a         1213 c         1119 g         1690 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaa
//
LOCUS       DAZOZX010000198         5485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZOZX010000198 DAZOZX010000000
VERSION     DAZOZX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.201"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1630)
                     /locus_tag="ACHUPT_003834"
     CDS             complement(<1..1630)
                     /locus_tag="ACHUPT_003834"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0821196.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHUPT_003835"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHUPT_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHUPT_003836"
     CDS             complement(2541..3146)
                     /locus_tag="ACHUPT_003836"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0821197.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACHUPT_003837"
     CDS             complement(3317..>5485)
                     /locus_tag="ACHUPT_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR0821198.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAZOZX010000199         5479 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZOZX010000199 DAZOZX010000000
VERSION     DAZOZX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.202"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            523..1512
                     /gene="araF"
                     /locus_tag="ACHUPT_003838"
                     /pseudo
     CDS             523..1512
                     /gene="araF"
                     /locus_tag="ACHUPT_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1582..3096
                     /gene="araG"
                     /locus_tag="ACHUPT_003839"
     CDS             1582..3096
                     /gene="araG"
                     /locus_tag="ACHUPT_003839"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0821199.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3111..4097
                     /gene="araH"
                     /locus_tag="ACHUPT_003840"
     CDS             3111..4097
                     /gene="araH"
                     /locus_tag="ACHUPT_003840"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0821200.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4264..5064
                     /gene="otsB"
                     /locus_tag="ACHUPT_003841"
     CDS             4264..5064
                     /gene="otsB"
                     /locus_tag="ACHUPT_003841"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0821201.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            5039..5479
                     /locus_tag="ACHUPT_003842"
                     /pseudo
     CDS             5039..5479
                     /locus_tag="ACHUPT_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1352 a         1268 c         1467 g         1392 t
ORIGIN      
        1 tttgaattca tgatgaagtg atgattttat aaaacgtttt ttcatttttg cgagatggct
       61 ctcattatac gtgttctgtt aattaatgag cacagtgata attaatggcg aaatgaatga
      121 cgcgcatttt ccacatcttt gagttgtggt tattacacca tttcaaaaac tcaacgccag
      181 gtaatgcggc ctattgactg gttaaaaaga agacatcccg catgggtacc gaagacaaca
      241 aggatttcca ggctaatctt atggattaat ctgctgtgca ttcaacaatt tgtctgacaa
      301 attggctttt ccttatgtct tttcccgcta aatttatgca cgttctcact gtaattctgc
      361 gatgtgatat tgctctccta tggagaatta atttctcgct aaaactatgt caacacggtc
      421 acttatcttt tagataaaag gtaatgcttt gttttccgat taatttaacg aatgtcattc
      481 gtttttgccc tacacaaaac gacactaaag ctggagagaa ccatgcacaa atttactaaa
      541 gccctggcag ccattggtct ggcagccgtt atgtcacaat ccgctatggc ggagaacctg
      601 aagctcggtt ttctggtgaa gcaaccggaa gagccgtggt tccagaccga atggaagttt
      661 gccgataaag ccgggaagga tttagggttt gaggttatta agattgccgt gccggatggc
      721 gaaaaaacat tgaacgcgat cgacagcctg gcagccagtg gcgcaaaagg tttcgttatt
      781 tgtactccgg acccgaaact cggctctgcc atcgtcgcga aagcgcgtgg ctacgatatg
      841 aaagtcattg cagtggatga ccagtttgtt aacgccaaag gtaagccaat ggataccgtt
      901 cctctggtga tgatggcggc gactaaaatt ggcgaacgtc agggctagga actgtataaa
      961 gagatgcaga aacgtggctg ggatgtcaaa gaaagcgcag tgatggcgat taccgccaac
     1021 gaactggata ccgcccgccg ccgtacaacg ggttctatgg acgcactgaa agctgcagga
     1081 ttcccggaaa aacaaattta tcaggtacct accaaatcta acgacatccc gggggcattt
     1141 gacgctgcca actcaatgct ggttcaacat ccggaagtta aacattggct gatcgtcggt
     1201 atgaacgaca gcaccgtact tggcggcgta cgtgcaaccg aaggtcaggg ctttaaagca
     1261 gccgatatca tcggtattgg tattaacggt gtggatgcag taagcgaact gtctaaagca
     1321 caggcaaccg gcttctacgg ttccctgctg ccaagcccgg acgtacatgg ctataaatcc
     1381 agcgaaatgc tttacaactg ggtagcaaaa gacgttgaaa cgccaaaatt taccgaagtt
     1441 acagacgtgg tactgatcac gcgtgacaac tttaaagaag aactggagaa aaaaggttta
     1501 ggcggtaagt aatttgccgg aaaaattccc ctctgcatga tgcagagggg gtgtgaacga
     1561 ccagtgattc acggagacgt tatgcaacag tctaccccgt atctctcatt tcgcggcatc
     1621 ggtaaaacgt ttcccggcgt taaggcgctg acggatatta gttttgactg ctatgccggt
     1681 caggttcatg cgttgatggg tgaaaatggc gcaggaaaat caactctctt aaaaatcctc
     1741 agcggcaact atgcgccaac cacgggttct gtagtgatta atgggcagga aatgtccttt
     1801 tccgacacga ccgcagcact taacgcgggt gtggcgatta tttaccagga actgcatctc
     1861 gtgccggaaa tgaccgtcgc ggaaaacatc tatctcggcc agctgccgca taaaggcggc
     1921 attgtgaatc gctcattgct gaattatgag gcgggtttac aacttaaaca tcttggtatg
     1981 gatattgacc cggacacgcc gctgaaatat ctcgccattg gtcagtggca gatggttgaa
     2041 atcgccaaag cgctggcgcg taacgctaaa attatcgcct ttgatgagcc aaccagctcc
     2101 ctttctgccc gcgaaatcgg caatcttttc cgcgttattc gtgaactgcg aaaagagggg
     2161 cgggtgatct tatacgtttc tcaccgtatg gaagaaatat ttgccctcag cgatgccatc
     2221 actgtcttta aagatggacg ttatgtcaaa acctttaccg atatgcagca ggttgaccac
     2281 gacgcgctgg tgcaggcgat ggtcgggcgc gacattggcg atatctacgg ctggcaacct
     2341 cgtagttatg gcgaggagcg cctgcgtctt gatgctgtga aagcactagg cgtgcgtacg
     2401 ccaataagtc tggcggttcg cagtggtgaa attgttgggc tgtttggtct ggtaggggcg
     2461 gggcgtagcg aattaatgaa aggcttgttt ggcgggacgc aaatcaccgc cggtcaggtt
     2521 tatatcgacc aacagccgat cgatattcgt aaaccgagcc acgccattgc cgcaggcatg
     2581 atgctctgcc cggaagctcg caaagcggaa ggcattattc ccgtgcactc cgttcgcgac
     2641 aatatcaaca tcagtgccag acgcaaacat gtgctcggcg gttgtgtaat caacaacagt
     2701 tgggaagaaa acaatgccga tcagcacatt cgttcgctca acatcaaaac gccgggcgcg
     2761 gagcaactga tcatgaatct ctcaggcgga aatcagcaaa aagccattct gggccgctgg
     2821 ttatcggaag agatgaaggt cattttgctg gatgaaccta cgcgcggcat tgatgttggc
     2881 gctaagcacg aaatatataa cgtaatttat gcgctggcgg cgcagggcgt ggcggtgctg
     2941 tttgcctcca gcgacttacc tgaagtcctc ggcgttgccg accggattgt ggtgatgcgg
     3001 gaaggcgaaa tcgccggtga attgttacac gagcaggcag atgagcgtca ggcactgagc
     3061 cttgcgatgc ctaaagtcag ccaggcagtt gcctgagtaa ggagagtatg atgtcttctg
     3121 tttctacatc ggggtctggc gcacctaagt cgtcattcag cttcgggcgt atctgggatc
     3181 agtacggcat gctggtggtg tttgcggtgc tctttatcgc ctgtgccatt tttgtcccaa
     3241 attttgccac cttcattaat atgaaagggt tgggcctggc aatttccatg tcggggatgg
     3301 tggcgtgtgg catgttgttc tgccttgctt ccggtgactt tgacctttct gtcgcctccg
     3361 taattgcctg tgcgggtgtc accacggcgg tggttatcaa cctgactgaa agcctgtgga
     3421 ttggcgtggc agcgggtttg ttgctgggcg ttctctgtgg cctggtcaat ggctttgtta
     3481 tcgccaaact gaaaataaat gctctgatca cgacactggc aacgatgcag attgttcgag
     3541 gtctggcgta catcatttca gacggtaaag cggtcggtat cgaagatgaa agcttctttg
     3601 cccttggtta cgctaactgg ttcggtctgc ctgcgccaat ctggctcacc gtcgcgtgtc
     3661 tgattatctt tggtttgctg ctgaataaaa ccacctttgg tcgtaatacc ctggcgattg
     3721 gcgggaacga agaggctgcg cgtctggcgg gtgtaccggt tgttcgcacc aaaattatta
     3781 tctttgttct ctctggcctg gtatctgcga tagccggaat tattctggct tcacgtatga
     3841 ctagtgggca gccaatgacg tcgattggtt atgagcttat tgttatctcc gcctgcgttt
     3901 taggtggcgt ttctctgaaa ggtggcatcg gaaaaatctc atatgtggtg gcgggtatct
     3961 taattttagg caccgtggaa aacgccatga acctgcttaa tatttctcct ttcgcgcagt
     4021 acgtggttcg cggcttaatc ctgctggcag cggtgatctt cgaccgttac aagcaaaaag
     4081 cgaagcgcac tgtctgatgc ttttttccac aacaacttaa cgttttttcc caccacagcc
     4141 agccgccaca acggttggct gttcttcgtt gcaaatggcg acctccgtca cactgtctat
     4201 acttacatgt ctgtaaagcg cggtctgcgc aacacaataa gaaaagagaa ggaggagagc
     4261 cgggtgacag aaccgttaac cgaaacccct gaactatccg cgaaatatgc ctggtttttt
     4321 gatcttgatg gaacgctggc ggaaatcaaa ccgcatcccg atcaggtcgt cgtgcctgac
     4381 aatattctgc aaggactaca gctactggca accgcaagtg atggtgcatt ggcattgata
     4441 tcagggcgct caatggtgga gcttgacgca ctggcaaaac cttatcgctt cccgttagcg
     4501 ggcgtgcatg gggcggagcg ccgtgacatc aatggtaaaa cacatatcgt tcatctgccg
     4561 gatgcgattg cgcgtgatat tagcgtgcaa ctgcatacgg tcatcgctca gtatcccggc
     4621 gcggagctgg aggcgaaagg gatggctttt gcgctgcatt atcgtcaggc tccgcagcat
     4681 gaagacgcat taatgacatt agcgcaacgt attactcaga tctggccaca aatggcgtta
     4741 cagcagggaa agtgtgttgt cgagatcaaa ccgagaggta ccagtaaagg tgaagcgatt
     4801 gcagccttta tgcaggaagc tccttttatc gggcgaacgc ccgtatttct gggcgatgat
     4861 ttaaccgatg aatctggctt cgcagtcgtt aaccgactgg gcggaatgtc agtaaaaatt
     4921 ggcacaggtg caactcaggc atcatggcga ctggcgggtg tgccggatgt ctggagctgg
     4981 cttgaaatga taaccgccgc attacaacaa aaaagagaaa ataacaggag tgatgactat
     5041 gagtcgttta gtcgtagtat ctaaccggat tgcaccacca gacgagcacg ccgccagtgc
     5101 cggtggcctt gccgttggca tactgggggc actgaaagcc gcaggcggac tgtggtttgg
     5161 ctggagtggt gaaacaggga atgaggatca gccgctaaaa aaggtaacat tacgtgggcc
     5221 tcttttaacc tcagcgaaca ggaccttgac gaatactaca accaattctc caatgccgtt
     5281 ctctggcccg cttttcatta tcggctcgat ctggtgcaat ttcagcgtcc tgcctgggac
     5341 ggctatctac gcgtaaatgc gttgctggca gataaattac tgccgctgtt gcaagacgat
     5401 gacattatct ggatccacga ttatcacctg ttgccctttg cgcatgaatt acgcaaacgt
     5461 ggggtgaata atcgcattg
//
LOCUS       DAZOZX010000200         5398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZOZX010000200 DAZOZX010000000
VERSION     DAZOZX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.203"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..497
                     /locus_tag="ACHUPT_003843"
     CDS             <1..497
                     /locus_tag="ACHUPT_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR0821202.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUPT_003844"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUPT_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHUPT_003845"
     CDS             2500..3114
                     /locus_tag="ACHUPT_003845"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0821203.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHUPT_003846"
     CDS             3114..3443
                     /locus_tag="ACHUPT_003846"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0821204.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUPT_003847"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUPT_003847"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0821205.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHUPT_003848"
     CDS             4529..4840
                     /locus_tag="ACHUPT_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0821206.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHUPT_003849"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHUPT_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZOZX010000201         5347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZOZX010000201 DAZOZX010000000
VERSION     DAZOZX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.204"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHUPT_003850"
     CDS             <1..171
                     /locus_tag="ACHUPT_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821207.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHUPT_003851"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHUPT_003851"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0821208.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUPT_003852"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUPT_003852"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0821209.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUPT_003853"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUPT_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0821210.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUPT_003854"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUPT_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0821211.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1319 a         1188 c         1400 g         1440 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tattttt
//
LOCUS       DAZOZX010000202         5340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZOZX010000202 DAZOZX010000000
VERSION     DAZOZX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.205"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUPT_003855"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUPT_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0821212.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHUPT_003856"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHUPT_003856"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0821213.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUPT_003857"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUPT_003857"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0821214.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUPT_003858"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUPT_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0821215.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAZOZX010000203         5278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZOZX010000203 DAZOZX010000000
VERSION     DAZOZX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.206"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(58..1341)
                     /gene="aroA"
                     /locus_tag="ACHUPT_003859"
     CDS             complement(58..1341)
                     /gene="aroA"
                     /locus_tag="ACHUPT_003859"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0821216.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1412..2500)
                     /gene="serC"
                     /locus_tag="ACHUPT_003860"
     CDS             complement(1412..2500)
                     /gene="serC"
                     /locus_tag="ACHUPT_003860"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0821217.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2699..3325)
                     /locus_tag="ACHUPT_003861"
                     /pseudo
     CDS             complement(2699..3325)
                     /locus_tag="ACHUPT_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3455..5215
                     /gene="ycaO"
                     /locus_tag="ACHUPT_003862"
     CDS             3455..5215
                     /gene="ycaO"
                     /locus_tag="ACHUPT_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0821218.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1346 a         1358 c         1263 g         1311 t
ORIGIN      
        1 acaatgtgcc gacgttttga taaagaaaga tttggctatt tattgcccgt tgttcattca
       61 ggctgcctgg ctaatccgcg ccagctgctc gaaataatcc ggaaatgttt tggccgtgca
      121 tttgggatca agaatcgtca ctggtgtatc tgacaacgcc accagcgaga aacacatcgc
      181 catccggtga tcattgtatg tcgcgatctc ggcaaagttc agtttttccg gaggagtgat
      241 acgaatgtaa tcgtgcccct cttccacttc cgcgccgact ttacgcagtt ctgttgccat
      301 cgcaaacagg cgatcggtct ctttaacacg ccagttatag atattgcgca gcgtggtggt
      361 gccttttgca aataacgccg ccgtggcaat ggtcatcgcc gcatcaggaa tatggttcat
      421 atccatatca atagcgttca gttcaccacg cgtgcaggaa atataatcat cgccccagca
      481 aatggtcgcg cccatttttt ccagcacatc agcaaagcga atatcaccct gcatactgtt
      541 acgtccaata ccggtcactt ttacagtgcc gcctttgatt gctgctgctg ccaggaagta
      601 agaagccgaa gatgcatcgc cttcgaccaa ataagtaccc ggagactgat aagactgccc
      661 gccttttacg acaaattgtt gatagtgctg attttcaatt tcaacaccaa acgtcttcat
      721 cagattgagt gtgatgtcga tataaggttt agaaaccaga tcgcctttaa tacgaatcac
      781 cgtatcttcc ggcgcaagag gcgcagtcat taacagtgcg gtgaggaatt ggctggaaac
      841 ggagccatca acgtcaacgt tgccgccagt aaagccgccc tgtaaacgca acggcggata
      901 attttcttgt tccaggtaag tgatcttcgc cccgcccagg cgcagcgcat ccaccagatg
      961 accaatcggg cgctctttca tacgcggctc accggtcagc acaatatcat tgctatccag
     1021 acaaagagct gccgccagcg gacgcattgc cgttccggcg ttaccgagga acaactccag
     1081 ggcaccttct gcgtgtaatg gaccgccgtt accgataatt tcgcaacgcg tacgatcggc
     1141 tgaaagcgta tagcttaacc ctaacgctgt taatgcattc agcatatggc gcacgtcgtc
     1201 gctatccagc agattggtta atactgtttt gccgtgtgct aatgccgcaa gcaataaagc
     1261 gcggttagaa acgctcttgg aaccgggcag attaatagtg ccatcgacac gagcgatggg
     1321 ttgtaacgtc agggattcca tgaactcaac tctctacaac agaaataaaa accccacagg
     1381 ctggctgtgg ggattaagca aaatttcggc attaaccgtg acggcgttcg aactcaacca
     1441 tgaagtctgt cagcgcttta acgccttcca gcggcatggc attataaata gacgcgcgca
     1501 ttccgccgac cacacggtgt cctttcagtg catgaaggcc tgcagcaaaa gactcttcaa
     1561 ggaacaattt gtcaagcgca ctgtccgcca actggaacgg cacgttcatc cgcgaacggt
     1621 tagctttcgc cacgtcattg cggtagaaat cgctgttatc aatcacccca tatagcagtt
     1681 ctgctttttg ctgattgatt ttatccattt cagctacacc gccgttcgct ttcagccatt
     1741 taaagaccag accagataga taccaggcaa atgtcggcgg cgtgttaaac atggagccgt
     1801 tatcgttgag gatggaataa tccagaatcg acggacacgc gatattcgct ttgcccagca
     1861 aatcttcacg aacgatgacg attgtcaggc cagccgggcc gatatttttc tgcgcgccag
     1921 cgtaaattac accataacgg ctgacgtcaa tcggacggga aagaatggtt gaagagaagt
     1981 cggcggcgac caccacatct ttgccgaagt ctggcgtctc gtcgatggcg ataccgtcga
     2041 tggtttcatt cgggcagtaa tgcatataag ccgcattatc agagagttgc cattcacgca
     2101 ttggcttaac cgcgcgcaga ccatcaacag tcactttggc gtcaaagaca ttaggcgtgc
     2161 agtatttttt cgcttcttta atggcacttg ccgcccagta accggcatca acataatctg
     2221 cggtggtttt atcaccgaga atattcagcg gtaccgcagc aaactgaccg cgaccaccgc
     2281 catggcagaa taataccttg tagttggagg ggacattaag aagatcgcga aaatccttct
     2341 cggcttcctc tgcaacctga atgaactctt tgccacggtg actcacttcc atcaccgacg
     2401 taccaagacc gttccagtcg cgcagttcct gttgagcctg tttaagcacc tctgccggta
     2461 gcattgccgg accagaacta aaattgaaga tttgagccat ttcccctcac cacgttgcgt
     2521 tgctattaac ccgcggtcaa tcgattgcga ccacaggtaa tacattattc ctgtggctat
     2581 cggttttatc attcagtgac acacatcgca atgagtaaaa ccgttgaggg caaaaatgtg
     2641 gcttgcatca cacaaaagaa tctctcggat tattagcaaa aagccgatgt ttttactgtc
     2701 aggtcacccg ttttgcccga cttgccttcg tccattctgg atttgcacag cgcggacata
     2761 attgtttttc ccccgcgttc atatgaatga tttcactaca acgaatgcag gcatagtccg
     2821 ccgactccgg caccactttg taacgctgcg tacaatcact gggtaaaata agtaaacccg
     2881 gtttcacctt gtcatcttca aagaaataga cactgatttg cccgttggtt tcgagaatcg
     2941 ccagacgtac ctgccccagc tgctccacgc cacgcaatcg tagctccata aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaacc agtacaaagg
     3241 tataaagact acgtagtgca acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca
     3301 tacggtggag atcgaacgcc ttcattttta tgctcttaac gtggtttatc cgctaaaagc
     3361 atagtcaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa aacccgtatt
     3421 attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta tccccggcaa
     3481 agatgccgct ctggaagatt ccatcgctcg cttccagcaa aaactttcag acctcggctt
     3541 tcagattgaa gaggcctcct ggctgaatcc cgtgcctaac gtctggtctg tacatattcg
     3601 cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaga aagcggcgct
     3661 ggcttctgca ctcggtgaat atttcgagcg tctctcaacc aactactttt ttgctgattt
     3721 ctggctgggc gaaaccatcg ccaacggtcc atttgtgcat tatcccaacg aaaaatggtt
     3781 cccactgagc gaaaatgacg atgtgccaga agggctgctc gatgaccgtc tgcgcgcatt
     3841 ttacgatccg gagaatgaat tgaccggcag tatgctgatt gacctacaat ccggtaacga
     3901 agatcgtggt atttgcggtc tgccgtttac gcgccagtcc gacaatcaga ccgtttatat
     3961 tccgatgaat atcattggta acctgtacgt ttctaacggt atgtccgctg gcaatacccg
     4021 caacgaagca cgcgttcagg ggttgtccga agttttcgaa cgctacgtga aaaaccgcat
     4081 tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcgc gttacccagc
     4141 ggtggttgaa gcgatcgaaa cactggaaac agaaggtttc ccgatcttcg catatgatgg
     4201 ttcccttggc ggccagtatc cggtgatttg cgtggtactg tttaatcctg caaacggcac
     4261 ctgctttgcc tctttcggtg cgcatcctga ttttggcgta gcactggaac gtaccgtgac
     4321 cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg tttactccgc caaccttcga
     4381 tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac tttatcgatt ccagcggttt
     4441 aatctcctgg gacctgttca agcaggatgc cgattatccg tttgtggact ggaatttctc
     4501 cggcaccacg gaagaagagt ttgctacgct gatggctatc ttcaacaaag aagataaaga
     4561 agtttatatt gccgattacg agcatctggg cgtttatgct tgccgtatta tcgtgcctgg
     4621 catgtccgat atttatccgg ctgaagatct gtggctcgcg aataacagta tgggcagcca
     4681 tttacgtgaa acgattcttt cgctaccagg cagcgagtgg gaaaaagaag attacctgaa
     4741 cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt
     4801 gggtctggcg accgggtcgg ataacggttg gtacaccctg cgtatcggtg aattaaaagc
     4861 catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg aatggacgat
     4921 ggagtttaac tcatcagtat ttagtccgga acgcgccaac tattatcgct gcctgcaaac
     4981 gttgttatta ctggcacagg aagaagatcg ccagccgctg caatatctga atgcgtttgt
     5041 tcgcatgtac ggcgcagatg ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc
     5101 gttttacggc ctgcaaccgg tagatagcga tctgcacgcg tttgctgcac atcagtcgtt
     5161 gttgaaggcc tacgaaaagc tgcagcgcgc caaagcagca ttctgggcaa aataaaatca
     5221 aatagcctac gcaatgtagg cttaatgatt agtctgagtt atattacggg gcgttttt
//
LOCUS       DAZOZX010000204         5266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZOZX010000204 DAZOZX010000000
VERSION     DAZOZX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.207"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..435)
                     /locus_tag="ACHUPT_003863"
     CDS             complement(<1..435)
                     /locus_tag="ACHUPT_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821219.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGV"
     gene            complement(503..2452)
                     /locus_tag="ACHUPT_003864"
     CDS             complement(503..2452)
                     /locus_tag="ACHUPT_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0821220.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2537..2872)
                     /locus_tag="ACHUPT_003865"
     CDS             complement(2537..2872)
                     /locus_tag="ACHUPT_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0821221.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2872..3228)
                     /locus_tag="ACHUPT_003866"
     CDS             complement(2872..3228)
                     /locus_tag="ACHUPT_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0821222.1"
                     /translation="MAKIAGICFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3228..4724)
                     /locus_tag="ACHUPT_003867"
     CDS             complement(3228..4724)
                     /locus_tag="ACHUPT_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0821223.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4721..4882)
                     /locus_tag="ACHUPT_003868"
     CDS             complement(4721..4882)
                     /locus_tag="ACHUPT_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR0821224.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4892..5251)
                     /locus_tag="ACHUPT_003869"
                     /pseudo
     CDS             complement(4892..5251)
                     /locus_tag="ACHUPT_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1243 a         1548 c         1140 g         1335 t
ORIGIN      
        1 cactcccgac tcgccattaa cattaactcc agctctgata ttcccgtttt cttttacatc
       61 tacaaaaaac tggcctcttg gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc
      121 aaaatcatca tctttatata ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt
      181 tgcaaaatca aatacaccta tacttttgcc gaattcataa gaagcttttc tcaatataat
      241 cgcaccgctg gcagaagggg atgggcacga tatcctcagt tgtctgaatc catctgtatc
      301 caagtcaacg tcattatctg cagatgtttt aacgttacta ataaatcgtg aaacaatctc
      361 ctcatttgtt agtattttcg cctgagcaca gcaagaggtt aatattgcaa ccgtcattaa
      421 aaatggcttt atcacaaaac aaactccatt gttagcatgt tacgcgataa ttttgcctca
      481 tctttcacca caaaaaaaga actcaccttt taaatggaga atacccaaca tcatgggtga
      541 ttttcataaa gggatcgcct gttttcggca aatcgataac gcgcattcca ggcggcgcgt
      601 tgtcaaacgt caccttaagt tcgctgcggg ttgccggtga agatgcaagg cttaacagct
      661 gattcctttc cagtgataca ttcggggtgt agcgtttatc gggagataaa tacccatcac
      721 cacgtggcat ctgccatccg gtcatgtcat agacaaaatc atggaactgt ttgccccact
      781 cgtcgaactt atcattcaga ccaaatccac tgttcagtgc atcagccaca aaatttttaa
      841 caagccaggg atgttctttt tcaaactcct gcgccatcat gcccagttca agcaatccac
      901 caatcagact gatctttccg aggcccttta atttcagccc tgtttttcct gccgccgttg
      961 tccattgatt ctgggcgact gttgccgcac gaatacttgc caccgccttc acaccaatat
     1021 acagggcaga aaccgttgct attgttttta ctgcctgttc ccatcccccc attgctctga
     1081 caacctgatc aacctcctgc cagacttttt tcacaaccgg accgaccgtt tcccagttat
     1141 caattatgag atatgcacca ccgacgagaa gcgcaattaa tccctttgcc ggcgtcatat
     1201 tcatcacgct acccatgatt tttgttatgc gtgtcaatgt gccaatcgcc acgcccatcg
     1261 tcagtaatgc cgcaccagtt ttcgcgatgg tttttacgac ctcaggattt tctctgacaa
     1321 acgtgcgcac ttcttccaga aaaggcttca tctcttttat gccttcattg agtgaaggca
     1381 ggaaggtttc ccccagcgtg gaagaaatcg cattgatctg gttctgcagc agcaacaact
     1441 gattttctgt cgtcgcggca cgggcggcat attctttctg cattgagccg ccatactgct
     1501 gcgcatccgc cacacgatta aagttggttc gcaacaaatc cagattcgtg agcagcggcg
     1561 caatggcccc cagggattcc ttcccgaaca gcgctttcag cacagcagcc tgtttttctt
     1621 tcggcacttt tgccagagaa tccagtacgt gcagcatggc cccgcgggcg tctttctgca
     1681 tatccgccgc cagttttttc gggctaatgc gcaacgagcg gagcactttt ttctgcgatt
     1741 tggtggcaga atccctcgct gtcagcgaca acataaaatt ctttatcccc gtcgcagcaa
     1801 tttctgactc cacccccatt ccggcaatgg ttgcccccat tgcggcaatc tctccggagg
     1861 ccacacccgc aacactgcct aaagggccaa tacgggtcac aacatcagaa atctttttcg
     1921 cacttgcagg accggtattg ccaagatagt taatcttatc cgcaagccct gccacctctc
     1981 cctgtgtcag tttaaaggca gtgcgccact gcgccatcat ctgaccggat tcttctgccg
     2041 tggtgtcaaa ggccacgccc atcttcacgg catcgtcagt aaactgcatc agctcgtcac
     2101 gggcgatacc agcctgacca cccgccgcca caatttcggc gataccttcc gccgacatgg
     2161 gcagttctgt tgacaggtcg cgcacctgct ccgtcattgc cttaaacgct tccggcgtat
     2221 ccagaccatc caccactttc cggacatccg ccatttttga ttcaagggca atggcagatt
     2281 tgaccgggag cgccagcgcc cccagtactg cggttccggc acccgctgcg cccagtgaca
     2341 ggctggcaaa ctccttttta aaacctttca gctggcgctg cattcccttc agcggtgccg
     2401 ataccttatc cacggcagtg atgatcgcct tcagctgaaa actgtcagcc atgcttcatc
     2461 tcctcgttaa tgcggacggc ctcggcttcc agttctgtaa actgcgaaac agccacccgt
     2521 cgcagctcca gtggattcag tttccagaac caggcaacat tgtagagtcg tttccggagg
     2581 tgcttcccgt ctccgactgg gtaaaaaaac gcaggatctg catgctggtc ttaaaaatat
     2641 ccagttttgc catctgcgcc gccgatgaac acgggatccc cgccagcaac gggatatatt
     2701 tcagtgccac ctggctgtcc agtttaatac tgccttcacc ggaaataatg aaaggaaaac
     2761 ccagcgcctc gatttcgtca tacgtgggct cgcgcaactc cagcacatgc agcatttcgt
     2821 tatgcgctgt caccggtttt tttaaaacta tttctgtaac gttcattgcc attactgata
     2881 ccctccctct tcgccgtgga attcaagatc tgccgtgcct tcttcggcat tatgattagc
     2941 ctctccgtgc agccatgccg ccgaaagcac atacaccatg ccgtttgcca gctcagcggt
     3001 aatcgtcatc tggtctgagg tggtaatttt gtcgaccgga aagtttttgg gcactttaaa
     3061 cgtgccctta acgtaaggtg accgccaggt ctccttgtaa tccacatccc ctgccatgcc
     3121 gacgatgtca tcccggacat tggtgttcat cggcacttca atgccacctg tcagcgataa
     3181 ctgctgacca tccactttaa aaaaacagat tccggcgatc tttgccatta tgctgactcc
     3241 tctgaatact gaagacggaa ctggttaacc accgcaaaga cacgtaactg attaacataa
     3301 tccggcggga acagcgtatt cagtcggttc gggttatctg catcacgctc aactatcaga
     3361 tactgtttaa acagatcgta tttttccaca ataccggcac gctccatctg acgatatgtc
     3421 gccagaagtt cccctttgat aacggcagga gtgacaatcg cctgccccgg accaaaacgg
     3481 gtaccatcat ttgccagctt gtgacgtccg tacttgcttg tgatgaccga tttcagtttg
     3541 cgaagaacat atgcactggt atgcagagtt tcactgtcca gatagctgtt gtctgccacg
     3601 ccatacgcat tctttttgta ggtggttacg gaacgctgga tctgcaacgt gccgccttcc
     3661 acataagccg tcgccacgcc gtgagataaa agggtctgct gctctgtcat gatgaatcgc
     3721 ttacctttcg gtgccggaag catccccacc agctcccccg tctgtgtcgg acgggcagga
     3781 tcattccgga taaataccgc ttcacgggca aggcgactgg caaccagttc atcgacaggc
     3841 gactgggttt ctttttcgta accggcaagc gtgatatgtt gctgattatg catatctccg
     3901 gcaccaacca gctctgacag cgttcccagt tttgcggtat agacatgccc gtataactgg
     3961 cgcgcataac tccagcgacc gctgctgtca ttcatttcgg tcatcatcat attgatggag
     4021 gcggcatcgt tgaacggcag accgataaag tcgaatgcct catcgcccat agcagcaaca
     4081 gcggcggtaa gatcaggcgc tccgctaccg gcagcaccga cttccgtcac cacccgaagc
     4141 cctgctggca aaatctcacc accaccagaa ccataataat tcagacagac cggtaactca
     4201 ttaccataca gccccttatg acgggcagtc agcgtcacca cgcctgcatc agatgacgcc
     4261 gtaaacggca gggtgataac cccgtttacc gcttccttaa tcgcggtggc aaccgcagtg
     4321 gcatcatcgc cattcaccac aggcacctgt acacgggagc gcccgacata cagactcagg
     4381 gtgccgcttt cctctgcttc tccggcaacc gtcaccctga ccgtcgccgc cgcccctctg
     4441 gcttccggta ctgcaataac atacagttca ccgaaaggat ctgtctgacg gtagacttcc
     4501 accatacgcg ccagctggct ccccgcccca caaatctggc gggcataatc tgccgatggc
     4561 atcagcacca ggctgttaac ctcaatggct gcatcgttgc tggcatgccc gatcagtaat
     4621 gcaggcgcgc tggtcaccgc tgtatttgcc gcagaattgt tcatctcggc gtagaacaac
     4681 ggcaccagcg tattcgacgg aacagtatta aaacttatcg tcatgatttt ttagccttat
     4741 cctgtttaac acgtaccaca tcacccgccg ctatacggcg gaaccagtag ctgctttctt
     4801 ccacatttcg cccttcggaa ggcaaaaggt ctccacgggc agggtcagga accgaccgcc
     4861 ctttcagggg tttcacaaac atgagggact cttaattctg agggaaacgc atttccagat
     4921 ggtgctcaat gtcaccatcc gggccagtac caggatcgat gtaatccaca tcaatactca
     4981 gcagggataa atccggcagg gcattcacgt cctctgcctg tcgcgtgtct tcttccgtga
     5041 tttcatactt cgccgtaaaa tcaaactggt aatacagttc gtaacggttc agatccagca
     5101 gggtgccacc gacataaaca atctcgcccc cttgcgggtc cggcatccat ccaagcaggg
     5161 ctttccagag ttctctccgg acatcatgaa cggcgtcata ggctgcccac tgcccttttt
     5221 catcacgctc attgaggtgt actggcaata gcggacacta ccattt
//
LOCUS       DAZOZX010000205         5183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZOZX010000205 DAZOZX010000000
VERSION     DAZOZX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.208"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..746
                     /locus_tag="ACHUPT_003870"
     CDS             <1..746
                     /locus_tag="ACHUPT_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0821225.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHUPT_003871"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHUPT_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0821226.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="ACHUPT_003872"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="ACHUPT_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0821227.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="ACHUPT_003873"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="ACHUPT_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0821228.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5183
                     /locus_tag="ACHUPT_003874"
     CDS             5041..>5183
                     /locus_tag="ACHUPT_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR0821229.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1481 a         1103 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tac
//
LOCUS       DAZOZX010000206         5165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZOZX010000206 DAZOZX010000000
VERSION     DAZOZX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.209"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..222
                     /locus_tag="ACHUPT_003875"
     CDS             <1..222
                     /locus_tag="ACHUPT_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821230.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="ACHUPT_003876"
     CDS             complement(394..723)
                     /locus_tag="ACHUPT_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0821231.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="ACHUPT_003877"
     CDS             complement(728..913)
                     /locus_tag="ACHUPT_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0821232.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="ACHUPT_003878"
     CDS             complement(910..3549)
                     /locus_tag="ACHUPT_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0821233.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="ACHUPT_003879"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="ACHUPT_003879"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0821234.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4857..>5165
                     /gene="hslJ"
                     /locus_tag="ACHUPT_003880"
     CDS             4857..>5165
                     /gene="hslJ"
                     /locus_tag="ACHUPT_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0821235.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1308 a         1323 c         1204 g         1330 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattagccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaaccagca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga
     4861 agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca
     4921 aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg
     4981 gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga
     5041 tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga
     5101 cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg
     5161 ataac
//
LOCUS       DAZOZX010000207         5150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZOZX010000207 DAZOZX010000000
VERSION     DAZOZX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.210"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            189..446
                     /locus_tag="ACHUPT_003881"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHUPT_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUPT_003882"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUPT_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0821236.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUPT_003883"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUPT_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0821237.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUPT_003884"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUPT_003884"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0821238.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHUPT_003885"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHUPT_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHUPT_003886"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHUPT_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHUPT_003887"
     CDS             complement(3604..4419)
                     /locus_tag="ACHUPT_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0821239.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5150
                     /locus_tag="ACHUPT_003888"
     CDS             4516..>5150
                     /locus_tag="ACHUPT_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0821240.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1225 g         1376 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct
//
LOCUS       DAZOZX010000208         5107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZOZX010000208 DAZOZX010000000
VERSION     DAZOZX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.211"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..390
                     /locus_tag="ACHUPT_003889"
     CDS             <1..390
                     /locus_tag="ACHUPT_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0821241.1"
                     /translation="APFTVTPEMLEAAREARRQEIEAWRAEQEAKLFTFEWNGRIWNA
                     GPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDE
                     IYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(591..1448)
                     /gene="sitD"
                     /locus_tag="ACHUPT_003890"
     CDS             complement(591..1448)
                     /gene="sitD"
                     /locus_tag="ACHUPT_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0821242.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1445..2302)
                     /gene="sitC"
                     /locus_tag="ACHUPT_003891"
     CDS             complement(1445..2302)
                     /gene="sitC"
                     /locus_tag="ACHUPT_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0821243.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2299..3126)
                     /gene="sitB"
                     /locus_tag="ACHUPT_003892"
     CDS             complement(2299..3126)
                     /gene="sitB"
                     /locus_tag="ACHUPT_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0821244.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3126..4040)
                     /gene="sitA"
                     /locus_tag="ACHUPT_003893"
     CDS             complement(3126..4040)
                     /gene="sitA"
                     /locus_tag="ACHUPT_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0821245.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4472..>5107)
                     /locus_tag="ACHUPT_003894"
     CDS             complement(4472..>5107)
                     /locus_tag="ACHUPT_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0821246.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1352 a         1250 c         1231 g         1274 t
ORIGIN      
        1 gccccattca ctgtgacacc agaaatgctg gaagcggcca gagaggccag acgtcaggaa
       61 attgaagcat ggcgcgcaga acaggaggcg aagctgttca cgtttgaatg gaacggtcgt
      121 atctggaatg ctggtcccga ctcactgggc cgcctgtccc cggtagtcat gctggcaaaa
      181 tctgtcacag cacaaacaca tatggcgtgg agcgatgccg ataatcagca ggtgaaactg
      241 tcgatgccgg aactggaaga actggcggca gcaatggtgc aggcgcaggt cgatcgcaac
      301 gatgagattt atcgccgtca gcgggagctg aaggaagagc tgaatagtct gaaggatttg
      361 aattcggtta ggaattttat cgtggaataa cagaagctgc ggcacgtcgt atgcaggaac
      421 gtgccgcggt tggctggtaa actttcgata gtgcgagtat tgaatgattt ctaggcgtta
      481 tcgattttac gtattttttg catgatagaa ttcgtacctc ctcccaacga ccttccatgg
      541 ctttccggac ttctgtaggc tggcggacga tgctgtcctc ggatgttgtg ctaattgggt
      601 gataacagat cctgtgtatg ggcgtttccc tttgtgcgtg cgttgatgct ggtaacggta
      661 aacgtcatga tgaacacgac cgcgaataag acgacaatcg tcggagctgg tgcgctgtcg
      721 ataaaaaagg acagccagac accagtcatt gacactatta ccgatatgcc agtcgccagt
      781 aggagcgcaa tatgaaagcg ttgtgtcagg agtactgcaa tggcgccagg cgcaatgagc
      841 agagaaatag agagaatgat acctactgct ttcagcgtcg ccacaatggt cagggaaacc
      901 atgcacagca gcccataatg cagccatctc gtatgcaaac cgcttacctg cgcctgttga
      961 taatcaaagc tgaacagcaa aaaatcccgc cattttacgc taataacaag tgtaaccagc
     1021 ccggcaataa tcaccgtctg gattatgtcg ccgatggtta tcccgagcat gtcgccgaaa
     1081 agaatatggt caagatgtac gtctggttta attgcaatat acaagatcag acctgcggca
     1141 aacatgccag aaaaaacaat ccccataact gtatcttgct tgattcggct gttgtctttc
     1201 aggtatcccg tcgccaccgc gcagaatact ccggcgacga atgcccctgt agccagaggt
     1261 aaccccagaa tccaggtaag cacgatgcca ggaaaaaccg catgactcat cgcgtcaccc
     1321 atcagtgccc aacctttcag gaccagaaag accgacagca gcgcacaggg tatggaaact
     1381 accagtgaaa tcagcagtgc gtgactcata aaggtgaact gcagtggctc cagtaataat
     1441 gccatcatga gcgatcctcc agcgctttat gcgcacgccg acgatttgcc aaaagtccat
     1501 gggtgggcgc aaatacaaat gctaaaagaa acagcagtgt ctgcgccacc acgatgattc
     1561 cacctgtcgc gccgtccaga aagtaactga cccacgcgcc gaggaaactg gttacactgc
     1621 caatagttac agcaattatg agcaggcggg gaaaacgatc ggttaacaac catgctgttg
     1681 caccgggagt caccaccaga cagatcacca gaaaggcacc aaccgtttgc agcgccgcaa
     1741 cggtcgaaac cgacagcagg gtgaagaaga ggatttttaa tctgcccgga tgtaatccaa
     1801 tagcacgggc gtgattctcg tcgaaaaagg tcaccataag atctttccat ttgaaaaaaa
     1861 gcacgatgat cgagagaatg ccaatgatag tgagttgcag gatatccgcc ggatcgattg
     1921 ccagaatatt gcccaggacg atggtctgaa tgttcacgga tgtcgggttc aatgacacca
     1981 taaacagccc caggccaaaa aaggacgaga agatcaggcc gatgatggca tcttctttta
     2041 agcgggtgcg ttggttaaga aacagcatgc tgcccgctgc cagtccgccg gaaaagaagg
     2101 ctcccagaga aaacggaagc cccagcatat atgcccccgc aacacccggg acaatagagt
     2161 gcgacagcgc atcgccaatc agcgaccagc cttttagcat cagatagcat gacaggaaag
     2221 cgcagagacc gccgaccatt gccgagaccc acatcgcatt gagcatatat tcatagctga
     2281 agggttccag tagcacgttc atcttgcttc cctctgcacc gctgacggtc gatgtgccac
     2341 aaaagggcgt tcatcgtcag taataatgct ttcttccgag ccgttaagtg tgacatggcg
     2401 cagtacgccg ctaaaagcca gctccaggtt ctcggctgta aaagtggtgt ctgtcggccc
     2461 gcttgccagg acggtgcctt tgaccatgac cgtataatcg caaaatgtcg tgactgagcc
     2521 gagattgtgc gttgagacaa gcatcgtttt gccttccgcg cgtaactccc tcaacaggct
     2581 gatgattttc gcttcggttt tcacatcaac gccggtaaaa ggttcatcca gcaggatcac
     2641 atcaccctgc tgtgcaattg ctctcgccag aaagacgcgt ttcttttgtc caccggaaag
     2701 ctcgccgatt tgacgatggc gaaaatccac catatcgaca cgttccagcg catcggtgac
     2761 aatctgacga tccctctttt tggctatacg caacataccc atatgcccat agcggcccat
     2821 catcaccaca tcttccacca gtacaggaaa tgaccagtca acctcttctg actgagggac
     2881 gtaggcaacc aggtttttct gtagcgcctg tcgcgtggga atacccagaa cagatatctt
     2941 tccgctggtc agacgcacaa atcccataat cgctttaaac aacgtcgatt ttccggaacc
     3001 gtttacccca accagagcgg caatagatcc acttggtact gtgaaggatg catcgcgcag
     3061 tgctgtgtgc ccgttacgcc aggtgacagt aacatcatta acgacaatgc ctgcagattg
     3121 catcattatt tctccctttt cccggcttta attccctgta ccagcgtact ggtagtaacc
     3181 ttaagaaggt cgatatacgt aggtaccggg ccgttttctg tgctcaggga atcgacatag
     3241 agcacaccgc cgtagtgtgc gccggtttca cgcgcaacct gacgcgctgg tttatcggaa
     3301 atcgtactct cgctaaagac tgccgggata tgatttttct taactatatc aacaacctta
     3361 cgtacctgct gcggtgttcc ttgttgatcg gcattaatcg gccacagata aagctctttt
     3421 agccccaaat cgcgtgcgag ataagaaaaa gccccttcac tggtgaccat ccatcgctga
     3481 ttctcaggga gttccgtaat ctgcttacgc aggggggcaa gggtttgggt aatcttggct
     3541 ttataagtat cggcattacg ttggtaggtt tgtgcatttg ccggatcgta ttttatcaac
     3601 gcatcacgaa tattatcgac gtaaatcaga gcattatctg gcgacatcca tgcatgggga
     3661 ttaggtttgc cctcataggg tccttcggtg atccctactg gcgtcacacc cgaagagaca
     3721 attacttctg gaaccccatt gagatgctgg taaaagcgtt ggaaccacaa ttccagattc
     3781 ataccattgg cgagaatcag ttgtgccccc tgcgcacgtt taatatcgcc aggggtaggc
     3841 tgatactcat gaatttctgc accaggcttg gttatggatg agacttctgc agcatctcca
     3901 gccacgtttt tggccatatc cgcgatgatg gtaaatgttg taatgacctt gaatttttca
     3961 gttgcacttg cctgaaatgc gatcgagcag gtgagggcga ggcaccccaa gagcatggtt
     4021 acttttttta ttgagtgcat aatttagtac ctatagtgag caaaattaga acaaacatag
     4081 caacggctat gttttatagc ataacctata caaaatgaaa ataattatca ttaacagctt
     4141 tgaggcctaa aacagggatt aggttgtttc ctgctggcga catgatattg aggaaaaaag
     4201 tagagcagct attaggcttt gaaatgactt tatgaggaaa attgttgatg gcaaaaatgg
     4261 ggcaaaaaag agtgggttat cgtagcttat tgttgtcgct gataatgttt aacatattga
     4321 aaaataaata aaatacttat gagtcagata gttgtgattt ttgcccttac ttgttcaggt
     4381 tgtattgttc tttctttgta atttgttgat tttcttgcat tatttcagtt ctctggtact
     4441 aaatggggca aattgggggc aaactttgca actacgataa ccgcgcattc aacatggcta
     4501 tctgttcgtc gttcatgtca tcaatccaca taccgtaaat ttcatacacc atttgcgcag
     4561 tttcatgccc catctggctg gctataaatg ccgggttcgc tcctgccgtc aacagccagc
     4621 aggcaaaagt atgtcgcgta tggtacggat tacggcggcg aataccagca cgttttactg
     4681 ctgcattcca tcttgtaccc aaactgctta ccgagtaata aggtttctgt ttttcgttac
     4741 acaccctggg catgaaaaca aaatgcagtt tttgtttttc agttctgccg tactcccgat
     4801 gataaaaagt gatttcgctt ttgcgatgat gcccggtcag tttgtattgc tccttcagtg
     4861 cttcaagagc cggctgcagt aatgctaccg tccggatccc ggcatttgtt tttgggggac
     4921 cgaacatatc aagtatcgtc aggtttcttc tgacattcac aattcccttc tcgaaatcca
     4981 catcctccca cgccagagct gccagttccc cgtgacgaag cccggagtaa acggcaaatt
     5041 tccacaagtt ttggctctgt cctttttcac tttccattaa tgcattgaat tctgttttag
     5101 ataacgg
//
LOCUS       DAZOZX010000209         5035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZOZX010000209 DAZOZX010000000
VERSION     DAZOZX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.212"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            307..1251
                     /gene="leuO"
                     /locus_tag="ACHUPT_003895"
     CDS             307..1251
                     /gene="leuO"
                     /locus_tag="ACHUPT_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0821247.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1406..1450)
                     /locus_tag="ACHUPT_003896"
     CDS             complement(1406..1450)
                     /locus_tag="ACHUPT_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821248.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1569..3293
                     /gene="ilvI"
                     /locus_tag="ACHUPT_003897"
     CDS             1569..3293
                     /gene="ilvI"
                     /locus_tag="ACHUPT_003897"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0821249.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3296..3787
                     /gene="ilvN"
                     /locus_tag="ACHUPT_003898"
     CDS             3296..3787
                     /gene="ilvN"
                     /locus_tag="ACHUPT_003898"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0821250.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3799..3885
                     /locus_tag="ACHUPT_003899"
     CDS             3799..3885
                     /locus_tag="ACHUPT_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0821251.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3967..4971
                     /gene="cra"
                     /locus_tag="ACHUPT_003900"
     CDS             3967..4971
                     /gene="cra"
                     /locus_tag="ACHUPT_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0821252.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1234 a         1139 c         1316 g         1346 t
ORIGIN      
        1 tgcattaaat atataaatta attattaaat aagcacattt aatccatttt gtagatgatt
       61 gagtattcgc ggtagttatg attagattgt tttcgcaaca aaacattatg gattattatg
      121 ctgtggtaaa tgactcattc cacggcaatg gattctgttt ttatcagaac ccgtatcttt
      181 atgttttccg aattttactc attttgcttt ttcttatttt atatgcatga taaatcatat
      241 tcttcaggat tatttctctg cattccaata agggaaaggg agttaagtgt gacagtggag
      301 ttaagtatgc cagaggtaca aacagatcat tcagagacgg cggagttaag caagccacag
      361 ctacgcatgg tcgatctcaa cttattaacc gttttcgatg ccgtgatgca ggagcaaaac
      421 attacccgtg ccgctcatgt tctgggtatg tcgcaacctg cggtcagtaa cgctgttgca
      481 cgcctgaagg tgatgtttaa tgacgagctt tttgttcgtt atggccgtgg tattcaaccg
      541 actgctcgcg catttcaact ttttggttca gttcgccagg cattgcaact agtacaaaat
      601 gaattgcctg gttcaggttt tgaacccgcg agcagtgaac gtgtatttca tctttgtgtt
      661 tgcagcccgt tagacagcat tctgacctcg cagatttata atcacattga gcagattgcg
      721 ccaaatatac atgttatgtt caagtcttca ttaaatcaga acactgaaca tcagctgcgt
      781 tatcaggaaa cggagtttgt gattagttat gaagacttcc atcgtcctga atttaccagc
      841 gtgccattat ttaaagatga aatggtgctg gtagccagca aaaatcatcc aacaattaaa
      901 ggcccgttac tgaaacatga tgtttataac gaacaacatg cggcggtttc gctcgatcgt
      961 ttcgcgtcat ttagtcaacc ttggtatgac acggtagata agcaagccag tatcgcgtat
     1021 cagggcatgg caatgatgag cgtacttagc gtggtgtcgc aaacgcattt ggtcgctatt
     1081 gcgccgcgtt ggctggctga agagttcgct gaatccttag aattacaggt attaccgctg
     1141 ccgttaaaac aaaacagcag aacctgttat ctctcttggc atgaagctgc cgggcgcgat
     1201 aaaggccatc agtggatgga agaacaatta gtctcaattt gcaaacgcta actgaatgca
     1261 gaataggcca gacatgaatg tctggtttat tctgcatttt ttattgaatg tagaatttta
     1321 ttctgaatgt gtggactctc tattttagga ttaattaaaa aaatagagaa attgctgtaa
     1381 gttgtgggat tcagccgatt tattatcaat ttaatccttc gtaatggagg attttatcgt
     1441 ttcttttcac ctttcctcct gtttattctt attaccctgc gtttatgtct ctggctgcca
     1501 attgcttaag caagatcgga cggttaatgt gttttacaca ttttttccgt caaacagtga
     1561 ggcaggccat ggagatgttg tctggagccg agatggtcgt ccgatcgctt atcgatcagg
     1621 gcgttaaaca agtattcggt tatcccggag gcgcagtcct tgatatttac gatgcattgc
     1681 ataccgtggg tggtattgat catgtattag ttcgtcatga gcaggcggcg gtgcatatgg
     1741 ccgatggcct ggcgcgtgcg accggggaag tcggcgtcgt gctggtaacg tcgggtccaa
     1801 gggcgaccaa tgcgattacc ggcatcgcca ccgcttatat ggattccatt ccattagttg
     1861 tcctttccgg gcaggtagcg acctcgttga taggttacga tgcctttcag gagtgcgaca
     1921 tggtggggat ttcgcgaccg gtggttaaac acagttttct ggttaagcaa acggaagaca
     1981 ttccgcaggt gctgaaaaag gctttctggc tggcggcaag tggtcgccca ggaccagtgg
     2041 tcgttgattt accgaaagat attcttaatc cggcgaacaa attaccctat gtctgccctg
     2101 agtcggtcag tatgcgttct tacaatccca ctactaccgg acataaaggg caaattaagc
     2161 gtgctctgca aacgctggta gcggcaaaaa aaccggttgt ctacgtaggc ggtggggcaa
     2221 tcatggcggg ctgccatcag cagttgaaag aaacggtgga ggcgttgaat ctgcccgttg
     2281 tttcctcatt gatgggaatg ggggcgtttc cggcaacgca tcgtcaggca ctgggcatgc
     2341 tgggaatgca cggtacctac gaagccaata tgacgatgca taacgcggat gtgattttcg
     2401 ccgtcggggt acgatttgat gaccgaacga cgaacaatct ggcaaagtac tgcccaaatg
     2461 ccactgttct gcatatcgat attgatccta cttccatttc taaaaccgtg actgcggata
     2521 tcccgattgt gggggatgct cgccaggtcc tcgaacaaat gcttgaactc ctgtcgcaag
     2581 aatccgccca tcaaccgctg gatgagatcc gcgactggtg gcagcagatt gaacagtggc
     2641 gcgctcttca gtgcctgaaa tatgacactc acagtgaaaa gattaaaccg caggcggtga
     2701 tcgagactct ttggcggttg actaatgggg acgcttacgt gacgtccgat gtcgggcagc
     2761 accagatgtt tgctgcgctt tattatccat tcgacaaacc gcgtcgctgg atcaattccg
     2821 gcggtctcgg cacgatgggt tttggtttac ctgcggcact gggcgtcaaa atggcgttgc
     2881 cagaagaaac cgtggtttgc gtcactggcg acggcagtat tcagatgaac atccaggaac
     2941 tgtctaccgc gttgcaatac gagttgcccg tactggtggt gaatctcaat aaccgctatc
     3001 tggggatggt gaaacagtgg caggacatga tctattccgg ccgtcattca caatcttata
     3061 tgcaatcact acccgatttc gtccgtctgg cggaagccta tggtcacgtc gggatccaga
     3121 tctctcatcc gcatgagctg gaaagcaaac ttagcgaggc gctggaacag gtgcgcaata
     3181 atcgcctggt gtttgttgat gttaccgtcg atggcagtga gcacgtctac ccgatgcaga
     3241 ttcgcggggg cggaatggat gaaatgtggt taagcaaaac ggagagaacc tgattatgcg
     3301 ccggatatta tcagtcttac tcgaaaatga atcaggcgcg ttatcccgcg tgattggcct
     3361 tttttcccag cgaggctaca acattgaaag cctgaccgtt gcgccaaccg acgatccgac
     3421 attatcgcgt atgaccatcc agaccgtggg cgatgaaaaa gtacttgagc agatcgaaaa
     3481 gcaattacac aagctggtcg atgtcttgcg cgtgagtgag ttggggcagg gcgcgcatgt
     3541 tgagcgggaa atcatgctgg tgaaaattca ggccagcggt tacgggcgtg acgaagtgaa
     3601 acgtaatacg gaaatattcc gtgggcaaat tatcgatgtc accccctcgc tttataccgt
     3661 tcaattagca ggcaccagcg ataagcttga tgcattttta gcatcgattc gcgatgtggc
     3721 gaaaattgtg gaagttgctc gctctggtgt ggtcggactt tcgcgcggcg ataaaataat
     3781 gcgttgagaa tgatctcagt gcgcaatttg cagcccaaca tgtcacgttg ggcttttttt
     3841 gcgaaatcag tgggaacctg gaataaaagc agttgccgca gttaattttc tgcgcttaga
     3901 tgttaatgaa tttaacccat accagtacaa tggctatggt ttttacattt tacgcaaggg
     3961 gcaattgtga aactggatga aatcgctcgg ctggcgggag tgtcgcggac cactgcaagc
     4021 tatgttatta acggcaaagc gaagcaatac cgtgtgagcg acaaaaccgt tgaaaaagtc
     4081 atggctgtgg tgcgtgagca caattaccac ccgaacgccg tggcagctgg gcttcgtgct
     4141 ggacgcacac gttctattgg tcttgtgatc cccgatctgg agaacaccag ctatacccgc
     4201 atcgctaact atcttgaacg ccaggcgcgg caacggggtt atcaactgct gattgcctgc
     4261 tcagaagatc agccagacaa cgaaatgcgc tgcattgagc accttttaca gcgtcaggtt
     4321 gatgccatta ttgtttcgac gtcgttgcca cctgagcatc ctttttatca acgctgggct
     4381 aacgacccgt tcccgattgt cgcgctggac cgcgccctcg atcgtgaaca tttcaccagc
     4441 gtggttggtg ccgatcagga tgatgccgaa atgctggcgg aagagttacg taagtttccc
     4501 gccgagacgg tgctttatct tggcgcgctg ccggagcttt ctgtcagctt cctgcgtgaa
     4561 caaggtttcc gtactgcttg gaaagatgat ccgcgcgaag tgcatttcct gtatgccaac
     4621 agctatgagc gggaggcggc tgcccagtta ttcgaaaaat ggctggaaac gcatccgatg
     4681 ccgcaggcgc tgttcacaac gtcgtttgcg ttgttgcaag gagtgatgga tgtcacgctg
     4741 cgtcgcgacg gcaaactgcc ttctgacctg gcaattgcca cctttggcga taacgaactg
     4801 ctcgacttct tacagtgtcc ggtgctggca gtggctcaac gtcaccgcga tgtcgcagag
     4861 cgtgtgctgg agattgtcct ggcaagcctg gacgaaccgc gtaagccaaa acctggttta
     4921 acgcgcatta aacgtaatct ctatcgccgc ggcgtgctca gccgtagcta atccgcgaac
     4981 aaaaatacgc gccaggtgaa tttccctctg gcgcgtagag tacgggactg gacat
//
LOCUS       DAZOZX010000210         4973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZOZX010000210 DAZOZX010000000
VERSION     DAZOZX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.213"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUPT_003901"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUPT_003901"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR0821253.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHUPT_003902"
     CDS             1020..1703
                     /locus_tag="ACHUPT_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR0821254.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHUPT_003903"
     CDS             1700..2161
                     /locus_tag="ACHUPT_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR0821255.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUPT_003904"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUPT_003904"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0821256.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUPT_003905"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUPT_003905"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR0821257.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUPT_003906"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUPT_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR0821258.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUPT_003907"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUPT_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR0821259.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHUPT_003908"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHUPT_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821260.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZOZX010000211         4889 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZOZX010000211 DAZOZX010000000
VERSION     DAZOZX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.214"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            185..1720
                     /gene="lsrA"
                     /locus_tag="ACHUPT_003909"
     CDS             185..1720
                     /gene="lsrA"
                     /locus_tag="ACHUPT_003909"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR0821261.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            1714..2742
                     /gene="lsrC"
                     /locus_tag="ACHUPT_003910"
     CDS             1714..2742
                     /gene="lsrC"
                     /locus_tag="ACHUPT_003910"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR0821262.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            2742..3734
                     /gene="lsrD"
                     /locus_tag="ACHUPT_003911"
     CDS             2742..3734
                     /gene="lsrD"
                     /locus_tag="ACHUPT_003911"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR0821263.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            3746..4769
                     /gene="lsrB"
                     /locus_tag="ACHUPT_003912"
                     /pseudo
     CDS             3746..4769
                     /gene="lsrB"
                     /locus_tag="ACHUPT_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            4796..>4889
                     /locus_tag="ACHUPT_003913"
     CDS             4796..>4889
                     /locus_tag="ACHUPT_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR0821264.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1112 a         1184 c         1345 g         1248 t
ORIGIN      
        1 tatctgtgat ggcaaccaca gtttgactct acgagcatga acaaacgcaa ccgtgaaaat
       61 caaaatagca taaattgtga tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta
      121 tgaacaaatt aaaagtagaa atacatttgt tcaaaactca cctgcaaaac tgaacggggg
      181 aaatatgcaa acgagtgata cccgcgcgtt accgctactt tgcgcccgct cggtttataa
      241 acagtattca ggggtcaatg tcctgaaagg catcgatttt acgttgcatc agggggaggt
      301 ccacgccctg ctcggcggca atggtgccgg taaatcgacg ttaatgaaga ttattgccgg
      361 tattacccct gctgatagcg gtacgctgga gattgggggc aacaactacg ccagattaac
      421 gccagttcat gctcatcagc tgggcattta tctcgttccc caggaaccgc tgcttttccc
      481 aagcctgtcg ataaaagaaa acatcctgtt tgggctggca aaaaaacagc tctccatgca
      541 gaaaatgaag aacttgctgg cggcgctggg ctgccagttt gatctgcata gtctggcagg
      601 atcgctggat gtcgccgatc gccaaatggt ggaaatcctc cgcgggctga tgcgcgactc
      661 gcggattctg atcctcgatg aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt
      721 gtttagtcgc ttgcaagagc tgcttgctac tggcgtgggt attgttttta tctcgcataa
      781 gctgccggaa attcgccaga ttgccgatcg aattagcgtg atgcgcgacg gaaccatcgc
      841 cttaagcggc aaaaccagcg aactgtctac cgacgacatt attcaggcca tcaccccagc
      901 ggtacgggaa aaatcgctct ctgccagcca aaaattatgg ctggagttac ctggtaaccg
      961 cccacaacat gccgtcggaa cgccggtgct gacactggaa aatctgactg gcgaaggctt
     1021 caggaatgtc agcctgacgc tcaatgccgg agaaattctg ggcctggctg ggctggtggg
     1081 agccggacgc acagaactgg ccgagacgct ctatggtctg cgtactttgc gtggcggacg
     1141 cattatgctg aatggtaaag agatcaataa attatccact ggagaacgtt tactgcgcgg
     1201 tctggtttat ctgccggaag atcgccagtc atccggactg aatctcgatg cttcgctggc
     1261 ctggaacgtc tgcgccctta ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga
     1321 taatgccacc ctggaacgtt atcgtcgggc gctgaatatt aaatttaacc aaccggaaca
     1381 agctgcacgg acattatccg gtagcaacca gcaaaaaatc ctcattgcca aatgcctgga
     1441 agcttcgccg caagcattga ttgtcgatga gccgacgcgc ggcgtggatg tctcggcccg
     1501 taatgatatc taccagctgt tgcgcagcat cgccgcacag aatgtggctg tgctgtttat
     1561 ctcctctgat atggaagaga tcgaactgat ggcagatcgt gtgtatgtga tgcatcaggg
     1621 cgaaattacc cactctgcac tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt
     1681 tgccttcggc gatagtcagc gtcaggaggc atcatgctga agtttattca gaacaaccgt
     1741 gaaatcacgg cattgctggc ggtggtgctg ctgtttgtat tacccggttt tctcgaccgc
     1801 cagtatttaa gtgtgcaaac gctgaccatg gtttatagca gcgcgcaaat cctgatcctg
     1861 ctggcaatgg gcgcgacgct ggtaatgctt acgcgcaata ttgatgtttc agtgggttcg
     1921 attaccggaa tgtgcgcggt gctgttgggg atgttactga acgcaggata ttcactacct
     1981 gttgcctgtg tcgcgacttt actgcttggt ttgctcgcgg gatttttcaa cggtgccctg
     2041 gtcgcgtggc taaagatccc tgccattgtt gccacccttg gcacgttagg gttgtacaga
     2101 ggcatcatgt tgctgtggac tggcggtaaa tggattgaag ggttacccgc cgaactgaaa
     2161 cagctctccg ccccgctgct gcttggcatt tcagcaattg gttggttgac gataattctg
     2221 gtggcattta tggcctggct gctggcgaag acggcgtttg gacgcagttt ttatgccacg
     2281 ggcgataatt tacagggcgc tcgtcaactg ggcgttcgta cggaagccat tcgcattgtg
     2341 gcattttcgt tgaacggctg catggcggca ctggcgggaa ttgtgtttgc ttcgcagatt
     2401 ggttttatcc tcaaccagac cggtaccggg ctggagatga aagcaattgc agcctgcgtg
     2461 ctgggcggca ttagtttgct cggtggttcc ggtgcgatca tcggtgcggt actcggcgca
     2521 tggttcctga cgcagatcga tagcgtactg gtgctgttgc gcattccggc atggtggaat
     2581 gattttatcg cgggtctggt tctgctggcg gtgctggtgt ttgatggacg cctgcgttgt
     2641 gcgctggaac gtaatctacg gcggcaaaaa tatgcccgct ttatgacgcc accgccatcc
     2701 gttaaacccg cttcgtcagg taaaaaacgg gaggccgcat aatgcgtatt cgctacggtt
     2761 gggaactggc tcttgccgca ctgctcgtta ttgagattgt cgcatttggt gcaattaacc
     2821 cgcgaatgtt agatctcaat atgttgctgt tcagcaccag tgactttatc tgcattggca
     2881 ttgtcgctct accgctgacg atggtgattg tcagtggcgg gatcgatatt tcgtttggtt
     2941 cgaccatcgg cctctgcgcc attgcattgg gcgtactgtt tcaaagtggt gtgccgatgc
     3001 cgctggcgat actcctgacc ttactgctcg gcgcattgtg cgggctgatc aacgccggat
     3061 taattatcta taccaaagtt aacccgctgg tgattacgct tggcacgctg tatctgtttg
     3121 ccggaagcgc tctgctgctt tccggtatgg ccggagcgac ggggtacgaa ggtattggtg
     3181 gattcccgat ggcgtttaca gatttcgcta acctggatgt gctgggactc cccgttccgc
     3241 tgattatctt cctgatatgt ctcctcgttt tctggctctg gctgcataaa acccatgccg
     3301 gacgtaatgt gtttttgatt gggcaaagcc cgcgcgtggc gctttatagc gcgattccag
     3361 ttaaccgcac cttatgtgcg ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg
     3421 tgctgctggt atcgtatttt ggttcagcac gttccgatct cggtgcgtcg tttctgatgc
     3481 ccgccatcac cgccgtggtg cttggcgggg ccaatattta tggtggttcc ggttccatta
     3541 tcggcaccgc cattgcggtt ttattagtgg gatatttgca gcaaggtttg caaatggcag
     3601 gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct gcttatcgtc gttgtcgtag
     3661 gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata
     3721 acccattgcc ataaaggata tcttcatgac acttcatcgc tttaagaaaa tcgccttact
     3781 tagcgctctt ggcattgccg caatctctat gaatgtgcag gccgcagagc gtattgcatt
     3841 tattcccaaa ctggttggcg tgggattttt taccagcggt ggcaacggcg cacaacaagc
     3901 gggtaaagag ctgagcgttg atgtgaccta cgacgggccg acagaaccca gtgtttctgg
     3961 tcaggtacag ttgattaata acttcgtcaa tcaaggttat aacgccatta ttcgtttctg
     4021 cggtttcgcc tgatggcttg tgtccggcac tgaaacgcgc catgcaacgt ggtgtgagag
     4081 tgctgacctg ggactctgat actaaaccgg agtgccgctc ttactacatt aatcagggaa
     4141 cgcccgccca gttgggaggt atgttggtgg atatggcggc gcgtcaggtg aataaagaca
     4201 aagccaaagt cgcgtttttc tactcaagcc ccaccgttac ggaccaaaac cagtgggtga
     4261 aagaagcgaa agcgaaaatc gccaaagagc atccaggctg ggaaattgtc actacgcagt
     4321 ttggctataa cgatgccact aaatcgttac aaaccgcaga aggaatatta aaagcgtata
     4381 gcgatctcga cgccattatc gcccccgatg ccaacgccct gcccgctgcc gcacaagccg
     4441 cagaaaacct gaaaaatgac aaagtagcga ttgtcggatt cagtacgcca aatgtgatgc
     4501 gcccgtatgt agagcgcggc acggtgaaag aatttggact gtgggatgtg gttcagcaag
     4561 gcaaaatttc agtgtatgtc gcggatgtat tattgaaaaa aggatcaatg aaaacgggcg
     4621 acaagctgga tatccagggc gtaggtcagg ttgaagtctc gccaaacagc gttcagggct
     4681 atgactacga agcggatggt aatggcatcg tactgttacc ggagcgcgtg atattcaaca
     4741 aagagaatat cggcaaatac gatttctgat gtgcattact taaccggagt aagttatggc
     4801 agatttagac gatattaaag atggtaaaga ttttcgtacc gatcaaccgc aacaaaatat
     4861 cccttttacc ctgaaaggtt gcggtgcgc
//
LOCUS       DAZOZX010000212         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZOZX010000212 DAZOZX010000000
VERSION     DAZOZX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.215"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUPT_003914"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUPT_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0821265.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHUPT_003915"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHUPT_003915"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR0821266.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUPT_003916"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUPT_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUPT_003917"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUPT_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0821267.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHUPT_003918"
     CDS             4169..4342
                     /locus_tag="ACHUPT_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821268.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUPT_003919"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUPT_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0821269.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUPT_003920"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUPT_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0821270.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHUPT_003921"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHUPT_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZOZX010000213         4744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZOZX010000213 DAZOZX010000000
VERSION     DAZOZX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.216"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            57..623
                     /locus_tag="ACHUPT_003922"
     CDS             57..623
                     /locus_tag="ACHUPT_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0821271.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            627..1202
                     /locus_tag="ACHUPT_003923"
     CDS             627..1202
                     /locus_tag="ACHUPT_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0821272.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1244..2296)
                     /locus_tag="ACHUPT_003924"
     CDS             complement(1244..2296)
                     /locus_tag="ACHUPT_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0821273.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2521..3441
                     /gene="lpxL"
                     /locus_tag="ACHUPT_003925"
     CDS             2521..3441
                     /gene="lpxL"
                     /locus_tag="ACHUPT_003925"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0821274.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3613..3798
                     /locus_tag="ACHUPT_003926"
                     /pseudo
     CDS             3613..3798
                     /locus_tag="ACHUPT_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3834..>4744
                     /locus_tag="ACHUPT_003927"
     CDS             3834..>4744
                     /locus_tag="ACHUPT_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0821275.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT         1209 a         1144 c         1150 g         1241 t
ORIGIN      
        1 attcattctc cgacacgcca ttttctttct ctcgcaaatt cgtattggat gcacttatgt
       61 cattcacaaa tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg
      121 caattattgt ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg
      181 atggctggta tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg
      241 ggctggttat tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt
      301 attcgcccat gactcgtctt gccgcaaaag ctggacatct cgcattgtgc ctattgctgt
      361 tcgccatcgg catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt
      421 ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg
      481 gtgccctgca tttttggctc gcgtggagcg tcgtagtact gtctgttatg cacggattta
      541 tggccctgaa gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt
      601 cgtcatctga ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt
      661 ccctgatgtt ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc
      721 agcacgcctt tgttaacttc cgcatacagc atcttggcta tagctggtta tatggcacct
      781 ttaaagattt cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga
      841 atgtgacaat taacaccacc agcgtcgata ctaaccacgc cgaacgcgat aaacatcttc
      901 gcagtgcgga ttttctcaat accacaaaat atccacaggc aacattcacc tccaccagcg
      961 tgaagaaaga cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca
     1021 aacccgtcac gctggaagcg aaattaattg gtcagggcga cgacccatgg ggcggtaaac
     1081 gtgcaggctt tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt
     1141 taggtccagc ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt
     1201 aagagtgaaa tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag
     1261 gaatacccag tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt
     1321 cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa
     1381 tctcactaca acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt
     1441 ggcagccatc atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat
     1501 gcgcgataat ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc
     1561 caataaaacg caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc
     1621 cctcgatatg ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac
     1681 aacgaatgcc gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct
     1741 cgactgcttt tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa
     1801 agtgccccac ttcatactca tagtggttac gcatgtcgat aaatagtgca tcgggatcgt
     1861 caagcatggc gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt
     1921 gaggatcgtc aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga
     1981 aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga
     2041 aggcatagag ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt
     2101 taatgccctc atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat
     2161 ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg
     2221 tggttcgcgg ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt
     2281 tgtgtaacac tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat
     2341 gatatagcaa ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa
     2401 attatttgtt atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg
     2461 cgggcgaaaa aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat
     2521 atgacgaatc tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg
     2581 ttgggtattg gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc
     2641 ggttgtggat taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat
     2701 cgcaacctgg aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg
     2761 aagaatttcg aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg
     2821 gaccgccgaa tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag
     2881 gcgcaaaaac gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg
     2941 cggcagtttg gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg
     3001 attgactggc tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa
     3061 gatttaaaag gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccagat
     3121 catgattacg gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg
     3181 accacgaccg gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt
     3241 ccacgccgta agccagatgg caaagggtat caattgatta tgctgccacc agagtgttct
     3301 ccgccactgg atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc
     3361 atcatgatgg caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa
     3421 ggcgttcctt cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt
     3481 accacagcaa atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt
     3541 cgggcgcata attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa
     3601 cagcggattg ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg
     3661 atcgtcgcct ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc
     3721 ttacccctct acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc
     3781 ggtattgtct tcagtataat gggttcctaa acacatcatt ttaggtattg aatatgagac
     3841 gggcaatcag ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc
     3901 gacaatctaa gctggttact gactggcttg ataagaaccc tgagttttac ttggattcat
     3961 ccttgagctt taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc
     4021 taggtgactt tttaaccgct atcgagaaag ggcttgttaa agctggtgat acgctactaa
     4081 tcgagagttt agaccgtctg tcacgtcagg atattgatat tgcttctgag ctattacgcc
     4141 gtatcttaag ggctggtgta gatgtagtca cactgagcga tggagaacat tacacacgag
     4201 aaagtttaaa agacccgtta gccctgatta aaagcattct gataatgcag cgggcgcatg
     4261 aggaaagttt gagaaagtct gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca
     4321 tctccgaagg tatcaaggta tctaggcgtt gtcctgcatg gttaaggctg aatgatgacc
     4381 gtcgaacatt cacaatcatt cctgataaag tggaagttgt taaacgagca tttgacctga
     4441 gattacaggg tttaagcttt tgggctatca ccagaacttt aaacgatgaa ggtcacttgt
     4501 cattgaatca atacacaccc aaacaaaagg ggtggtctga tactgctgta aagaaactgc
     4561 tcagaaatcg tgctgtgatt ggatgtttca cacctgctgg acgtgaagaa gttcagggtt
     4621 actaccccgc aataatcagt gagtcactat tctatcgagt acagcaactg aacacagggc
     4681 aatatggtag ggcttctgta tcaagtaatc cgctatcagt caacttgttc aggggtatca
     4741 tcaa
//
LOCUS       DAZOZX010000214         4603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZOZX010000214 DAZOZX010000000
VERSION     DAZOZX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.217"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHUPT_003928"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHUPT_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0821276.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHUPT_003929"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHUPT_003929"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0821277.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHUPT_003930"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHUPT_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0821278.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHUPT_003931"
     CDS             complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHUPT_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0821279.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAZOZX010000215         4477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZOZX010000215 DAZOZX010000000
VERSION     DAZOZX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.218"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            192..1049
                     /gene="focA"
                     /locus_tag="ACHUPT_003932"
     CDS             192..1049
                     /gene="focA"
                     /locus_tag="ACHUPT_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0821280.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1104..3386
                     /gene="pflB"
                     /locus_tag="ACHUPT_003933"
     CDS             1104..3386
                     /gene="pflB"
                     /locus_tag="ACHUPT_003933"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0821281.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3578..4318
                     /gene="pflA"
                     /locus_tag="ACHUPT_003934"
     CDS             3578..4318
                     /gene="pflA"
                     /locus_tag="ACHUPT_003934"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0821282.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4400..>4477)
                     /locus_tag="ACHUPT_003935"
     CDS             complement(4400..>4477)
                     /locus_tag="ACHUPT_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821283.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1143 a         1104 c         1082 g         1148 t
ORIGIN      
        1 gaaaaaattc aactcatttg ttaattttta aaatttattt ttatttggat aatcaaatag
       61 ttactccgta tttgcataaa aaccatgcga gttacgggcc tataagccag gcgagatatg
      121 atctatatca atttctcatc tataatgctt tgttagtatc tcgtcgccga cttaataaag
      181 agagagttag tgtgaaagct gacaaccctt ttgatctttt acttcctgct gcaatggcca
      241 aagtggccga agaggcgggt gtctataaag caacgaaaca tccgcttaag actttctatc
      301 tggcgattac cgccggtgtt ttcatctcaa tcgcattcgt cttctatatc acagcaacca
      361 ctggcacagg cacaatgccc ttcggcatgg caaaactggt tggcggcatt tgcttctctc
      421 tggggctgat tctttgtgtt gtctgcggag ccgatctctt tacttccaca gtgttgattg
      481 ttgttgctaa ggcgagtggg cgcatcacct ggtgtcagtt ggctaaaaac tggctaaatg
      541 tctattttgg caacctggtc ggcgcactgc tgtttgtact tttaatgtgg ctttccggcg
      601 agtatatgac cgcaaatggt caatggggac taaacgtcct acaaaccgcc gaccacaaag
      661 tgcaccatac ttttattgag gccgtctgtc ttggtatcct ggcaaacctg atggtatgtc
      721 tggcagtatg gatgagttat tctggccgca gcctgatgga caaagcgttc attatggtgc
      781 tgccggtcgc gatgtttgtt gccagcggtt ttgagcacag tatcgcaaac atgtttatga
      841 tcccgatggg tattgtaatc cgcgacttcg catctccgga attctggacc gctgtcggtt
      901 ctgcaccgga aaatttttct cacctgaccg tgatgaactt catcactgat aacctgatcc
      961 cggttacgat cggtaatatt atcggcggtg gtttgttggt tgggttgaca tactgggtca
     1021 tttacctgcg tgaaaacgat caccattaat ggttgtcgaa gtacgcagta aataaaaaat
     1081 ccacttaaga aggtaggtgt tacatgtccg agcttaatga aaagttagcc acagcctggg
     1141 aaggttttac caaaggtgac tggcagaatg aagtaaacgt ccgtgacttc attcagaaaa
     1201 actacactcc gtacgagggt gacgagtcct tcctggctgg cgctactgaa gcgaccacca
     1261 ccctgtggga caaagtaatg gaaggtgtta aactggaaaa ccgcactcac gcgccagttg
     1321 attttgacac cgctgttgct tccaccatca cctctcacga cgctggctac atcaataaag
     1381 cgttggaaaa agttgttggt ctgcagactg aagctccgct gaaacgtgct cttatcccgt
     1441 tcggtggtat caaaatgatc gaaggttcct gcaaagcgta caaccgcgaa ctggacccga
     1501 tgatcaaaaa aatcttcact gaataccgta aaactcacaa ccagggcgtg ttcgacgttt
     1561 acactccgga catcctgcgt tgccgtaaat ccggtgttct gaccggtctg ccagatgctt
     1621 atggccgtgg ccgtatcatc ggtgactacc gtcgcgttgc gctgtacggt atcgactacc
     1681 tgatgaaaga caaatacgct cagttcacct ctctacaggc tgatctggaa aacggcgtaa
     1741 acctggaaca gactatccgt ctgcgcgaag aaatcgctga acagcaccgc gctctgggtc
     1801 agatgaaaga aatggctgcg aaatacggct acgacatctc tggtccggct accaacgctc
     1861 aggaagctat ccagtggact tacttcggct acctggctgc tgttaagtct cagaacggtg
     1921 ctgcaatgtc cttcggtcgt acctccacct tcctggatgt gtacatcgaa cgtgacctga
     1981 aagctggcaa gatcaccgaa caagaagcgc aggaaatggt tgaccacctg gtcatgaaac
     2041 tgcgtatggt tcgcttcctg cgtactccgg aatacgatga actgttctct ggcgacccaa
     2101 tctgggcaac cgaatctatc ggtggtatgg gcctcgatgg tcgtaccctg gttaccaaaa
     2161 acagcttccg tttcctgaac accctgtaca ctatggggcc gtctccggaa ccgaacatga
     2221 ccattctgtg gtctgaaaaa ctgccgctga acttcaagaa attcgccgct aaagtgtcca
     2281 tcgacacctc ttctctgcag tatgagaacg atgacctgat gcgtccggac ttcaacaacg
     2341 atgactacgc tatcgcttgc tgcgtaagcc cgatgatcgt tggtaaacaa atgcagttct
     2401 tcggtgcgcg tgcaaacctg gcgaaaacca tgctgtacgc aatcaacggc ggcgttgacg
     2461 aaaaactgaa aatgcaggtt ggtccgaagt ctgaaccgat caaaggcgat ctcctgagct
     2521 acgatgaagt gatggagcgc atggatcact tcatggactg gctggctaaa cagtacatca
     2581 ctgcactgaa catcatccac tacatgcacg acaagtacag ctacgaagcc tctctgatgg
     2641 cgctgcacga ccgtgacgtt atccgcacca tggcgtgtgg tatcgctggt ctgtccgttg
     2701 ctgctgactc cctgtctgca atcaaatatg cgaaagttaa accgattcgt gacgaagacg
     2761 gtctggctat cgacttcgaa atcgaaggcg aatacccgca gtttggtaac aatgatccgc
     2821 gtgtagatga cctggctgtt gacctggtag aacgtttcat gaagaaaatt cagaaactgc
     2881 acacctaccg tgacgctatc ccgactcagt ctgttctgac catcacttct aacgttgtgt
     2941 atggtaagaa aactggtaac accccagacg gtcgtcgtgc tggcgcgccg ttcggaccgg
     3001 gtgctaaccc gatgcacggt cgtgaccaga aaggtgctgt agcgtctctg acttccgttg
     3061 ctaaactgcc gtttgcttac gctaaagatg gtatctccta caccttctct atcgttccga
     3121 acgcactggg taaagacgac gaagttcgta agaccaacct ggctggtctg atggatggtt
     3181 acttccacca cgaagcatcc atcgaaggtg gtcagcacct gaacgttaac gtgatgaacc
     3241 gtgaaatgct gctcgacgcg atggaaaacc cggaaaaata tccgcagctg accatccgtg
     3301 tatctggcta cgcagtacgt ttcaactcgc tgactaaaga acagcagcag gacgttatta
     3361 ctcgtacctt cactcaatct atgtaattag atttgactga aatcgtacag taaaaagcgt
     3421 acaataaagg ctccacgaaa gtggggcctt ttttagcacg agagcctttt ttgtcagcta
     3481 tctatacttt aaggtgactg ccaaaacaga ctcgacgtag ccttcgagct gcgcaccaac
     3541 acggcctcag atgggccaca tctggagaaa caccgcaatg tcagttattg gtcgcattca
     3601 ctcctttgaa tcctgtggaa ccgtagacgg cccaggtatt cgctttatca cctttttcca
     3661 gggctgcctg atgcgctgcc tgtattgtca taaccgcgac acctgggaca cgcatggcgg
     3721 taaagaagtt accgttgaag atttgatgaa ggaagtggtg acctatcgcc actttatgaa
     3781 cgcttccggc ggcggcgtta ccgcatccgg cggtgaagca atcctgcaag ctgagtttgt
     3841 tcgtgactgg ttccgcgcct gtaaaaaaga aggcattcat acctgtctgg ataccaacgg
     3901 ttttgttcgt cgttacgatc cggtgattga tgaactgctg gaagtaaccg acctggtaat
     3961 gctcgatctc aaacagatga acgacgagat ccaccaaaat ctggttggag tttccaacca
     4021 ccgcacgctg gagttcgcta aatatctggc gaacaaaaat gtgaaggtgt ggatccgcta
     4081 cgttgttgtc ccaggctggt ctgacgatga cgattcggca catcgcctcg gtgaatttac
     4141 ccgtgatatg ggtaacgttg agaaaatcga acttctcccc taccacgaac tgggcaaaca
     4201 caaatgggtg gcgatgggtg aagaatacaa actcgatggt gttaaaccac cgaagaaaga
     4261 gaccatggaa cgcgtgaaag gcattcttga gcagtacggt cataaggtaa tgttctaatt
     4321 tcctcgtaaa gcggcaactt tattgagttg ccgctttttt attccgcgct tcattctgct
     4381 acagcgtttt taacacatct tatagtgcat ctaccatatc ccgtacctga gataataacg
     4441 actgataata gctcgcgtga agttcttccc catcgaa
//
LOCUS       DAZOZX010000216         4381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZOZX010000216 DAZOZX010000000
VERSION     DAZOZX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.219"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(265..1011)
                     /gene="cutC"
                     /locus_tag="ACHUPT_003936"
     CDS             complement(265..1011)
                     /gene="cutC"
                     /locus_tag="ACHUPT_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0821284.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(1025..1591)
                     /locus_tag="ACHUPT_003937"
     CDS             complement(1025..1591)
                     /locus_tag="ACHUPT_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0821285.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1807..3540
                     /gene="argS"
                     /locus_tag="ACHUPT_003938"
     CDS             1807..3540
                     /gene="argS"
                     /locus_tag="ACHUPT_003938"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0821286.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3582..3626)
                     /locus_tag="ACHUPT_003939"
     CDS             complement(3582..3626)
                     /locus_tag="ACHUPT_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821287.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3717..4205
                     /locus_tag="ACHUPT_003940"
     CDS             3717..4205
                     /locus_tag="ACHUPT_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR0821288.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         1170 a         1075 c         1052 g         1084 t
ORIGIN      
        1 acaatgtaat cattaattgc taaggaataa cccagttgct atttagaata tatatttatc
       61 agttttagca atttaaatcc cataatcaat gtgaatataa aaaatatttt tagcacaacg
      121 ctatgcgcac tgtcacccgc tcaacagatc acggttatcg ttcgtttttt atactgctca
      181 gggataaaaa aaggcccctg ttgaaattgc aggggcctgg tacgagcaag catcatattg
      241 ggcgacatga tgcaacggta aaaatcattt ggcctgatgg cgttcaatga ttcctttcat
      301 ttcagcaacc gccgccccgt ctacgatata acgcgaatac tcgtccgcgt gttcatctga
      361 tgacatggac aatccttgat tacgataacg catcggtgag gcttgccacg ctcccgcgga
      421 gctatggact tccagcactc cggcatcgag gaagtggtgc aggttttctg cacggactcc
      481 tgctccagcc ataatgattg gagcatcacg atgggcaata agttccataa tttttgataa
      541 accttgcagt gcgtctgatt tttgccctga tgtcagtacc cgggcaatgc ccaattccgc
      601 aagattattg agtgtatata aagggttagc gcacatatcg aaggcgcgat gaaaagtcac
      661 tgccagcgga ccggcagcag ccattatttt ttccattcgt ggcatatcga cattcccgtc
      721 aacatcgaga acgcccgtca ccagtccagg aaaacctaat tcgcggaccg tgcgcacatc
      781 ctcaagaatg gcggcaaact caccgtcgct gtagcaaaaa tcaccaccgc gtgggcgaat
      841 tatcggatgc acagggatcg tcacccgctg gcgcacggat ttcagtaccc ccaacgacgg
      901 cgttaagccc ccctcttttg gggctgcgca taattcaact ctgtctgcgc cgttttgctg
      961 cgccgttagt gcacattcca tgctgtaaca gcaaatttcc agtaatgcca tttttgctcc
     1021 ttaattacgc tgactgctcg ctggcaacga tctcttcaat ggaccacgga tgaaacttaa
     1081 tagttgtttt gccatcggtg accgccagcg tcggattcgg taatcgctca tgctcacctt
     1141 ttggtgaact ggttttaact gagatccccg gtaggctgag tccttcatca gaaagcaatg
     1201 ccagcgcacg ggcattcagc gtttctggat cacccggcag aacgatttca atatgttccc
     1261 aaccttcgtg tgggtaacgt ttttccccgg gccacggtag ctccacattg gaaaactgcc
     1321 agtgcgcaac ctgtaccggt tcatgcaatt taaacagaca aatcggtctg ccattgatca
     1381 ttttttctga caaaagctcg ccacactgtt caaaaccgcg acgccagcgc tcagcggtga
     1441 cgttttgatg gcaacgcaaa gaaatgtgat cggcagtcag cggagtgata tccaacccca
     1501 gacggcggga aagttcatct aatgcgtggg taaatcgcgg taaatccgat gcaatatcct
     1561 gcagctcgtc gatagattgc cagttcgcca taatcactct tcgtctttca gtaaaagcgt
     1621 taatttaccc tgttgccctg tgccaaccaa ccgctgattt cacgccgctt ctgatgcaat
     1681 agtgaaaacg gcaatacgcc acgcgcacgt tgctgacgaa aacagccatt tgcagtatac
     1741 tcccgcccta atttctttaa ctggtgcggg caatttttgc tcgcttcatc aatgtaaggt
     1801 attccggtga atattcaggc tcttctctca gaaaaagtcc gtcaggccat gattgcggca
     1861 ggcgcgcctg cggattgcga accgcagatt cgtcagtcag caaaagttca gttcggcgac
     1921 tatcaggcta acggcatgat ggcagttgct aaaaaactgg gtatggcacc gcgacaatta
     1981 gcagagcagg tgctgactca tctggatctt aacggtatcg ccagcaaagt tgagatcgcc
     2041 ggtccaggct ttatcaacat tttccttgat ccggcattcc tggctgaaca tgttcagcag
     2101 gcgctggcgt ccgatcgtct cggtgttgct acgccagaaa aacagaccat tgtggttgac
     2161 tactctgcgc caaacgtggc gaaagagatg cacgtcggtc acctgcgctc taccattatt
     2221 ggtgacacag cagtgcgtac tctggagttc ctcggtcaca aagtgattcg cgcaaaccac
     2281 gtcggcgact ggggcactca gttcggtatg ctgattgcat ggctggaaaa gcagcagcag
     2341 gaaaacgccg gtgaaatgga gctggctgac cttgaaggtt tctaccgcga tgcgaaaaag
     2401 cattacgacg aagatgaaga gtttgccgag cgtgcacgta actacgtggt aaaactgcaa
     2461 agcggtgacg aatatttccg cgagatgtgg cgcaaactgg tcgacatcac catgacgcag
     2521 aaccagatca cttacgatcg tctcaacgtg acgctgaccc gtgatgacgt gatgggcgaa
     2581 agcctctaca acccaatgct gccaggaatt gtggcggatc tcaaagccaa aggtctggca
     2641 gtagaaagcg aaggggcgac cgtcgtattc cttgatgtgt ttaaaaacaa ggaaggcgaa
     2701 ccgatgggcg tgatcattca gaagaaagat ggcggctatc tctacaccac cactgatatc
     2761 gcctgtgcga aatatcgtta tgaaacactg catgccgatc gcgtgctgta ttacatcgac
     2821 tcccgtcagc atcaacacct gatgcaggca tgggcgatcg tccgtaaagc aggctatgta
     2881 ccggaatccg taccgctgga acaccacatg ttcggcatga tgctgggtaa agacggcaaa
     2941 ccgttcaaaa cccgcgcggg tggtaccgtg aaactggcag acctgctgga tgaagccctg
     3001 gagcgtgcac gtcgtctggt ggcagaaaag aacccggata tgccagctga cgagctggaa
     3061 aaactggcta acgcggttgg tattggtgcg gtgaaatatg cggatctctc caaaaaccgc
     3121 accacggact acatcttcga ctgggacaac atgctggcgt ttgagggtaa taccgcgcca
     3181 tacatgcagt atgcttacac gcgtgtattg tccgtgttcc gtaaagcaga aattaacgaa
     3241 gagcaactgg ctgcagctcc ggtaatcatc cgtgaagatc gtgaagcgca actggcagct
     3301 cgcctgctgc agtttgaaga aaccctcacc gtggttgccc gtgaaggcac gccgcatgta
     3361 atgtgtgctt acctgtacga tctggccggt ctgttctctg gcttctacga gcactgcccg
     3421 atcctcagcg cagaaaacga agaagtgcgt aacagccgtc tgaaactggc acaactgaca
     3481 gcgaagacgc tgaagctggg tctggatacg ctgggtattg agactgtaga gcgtatgtaa
     3541 tcgatttttc gtgagagtga agcctgatca gggttagccg atcaggcttt tttattgcca
     3601 tctaaatgta ttctgaaaac ggacatgcca ttgttttctc actgttgagt aagaggccag
     3661 aagcgtaata tccaggccca ggaaaacgat aacggttcaa tttaaggaat accgaagtgt
     3721 ttaaatttct tgtattaaca ttaggcatta tctcttgcca ggcttacgca gaagatacgg
     3781 ttatagtaaa cgaccatgac acttcagcca tcaaagattg ttggcaaaaa aattcagatg
     3841 atgatactga cattaacgtg atcaaatcat gcctgcgaca agaatacaat ctcgtcgatg
     3901 cgcaattaaa taaagcctat ggtgaagctt atcgttatat agaacaagtg ccacgcacag
     3961 gtgcaaaaaa acttgatact gaacaactta acttgcttaa aaaatcacag cgagcctggc
     4021 tggattttag ggacaaagaa tgtgaattaa tcctttcaaa tgaggatgtt caggatttaa
     4081 gtgaccccta ttctgaatca gaatggctat catgtatgat catacagacc aatacgcgaa
     4141 ctcgccagtt gcagctatac cgtaactctg aagattttta tccaagccct ttgacaagag
     4201 gataattcac atctttttgg catgttttgt tgcaagctat tcctgataaa tgattgcaac
     4261 aaaacatcca gccttttgac tgagttatta aacatactcg cgagcgcgta attttttgtc
     4321 tttcagcgat aattcacaat cacttcattc cgttgtacct ttagcggtgg aattaaccgc
     4381 c
//
LOCUS       DAZOZX010000217         4358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZOZX010000217 DAZOZX010000000
VERSION     DAZOZX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.220"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            473..3781
                     /gene="icsA"
                     /locus_tag="ACHUPT_003941"
                     /gene_synonym="virG"
     CDS             473..3781
                     /gene="icsA"
                     /locus_tag="ACHUPT_003941"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR0821289.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3925..4243
                     /locus_tag="ACHUPT_003942"
                     /pseudo
     CDS             3925..4243
                     /locus_tag="ACHUPT_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1325 a          744 c         1025 g         1264 t
ORIGIN      
        1 ttgggaacat aataaaatgg aatgttattc ttctctttaa gaaaacgata catttccact
       61 aaaaacaatt atgcagttat tgcattggag agtctggtag gaaagttgtg atatgaatac
      121 attcatgtta ttggctttgg ggattaacat caaggggcag atagaaccat tgggtgtata
      181 gaaaaacaaa gtggttaaac tttgggcaag cgtacagaaa gaactgaaaa gttgcggtct
      241 gaagcagact atcatagtaa aattattaat ttattatcga acatatagct ttccccctct
      301 ttttttcaaa gcaagacaca ggtaaatttc tcccgttgca ttgatatata acacagctct
      361 catgttttgg ttgaggcttt gtttaatatg tttttgcata tatcgtccct ttattccgga
      421 taaaacggaa tcttttcagg ggtttatcaa ccacttactg ataatatagt gcatgaatca
      481 aattcacaaa tttttttgta atatgaccca atgttcacag gggggggccg gagaattacc
      541 tacggtaaag gaaaaaacat gcaaattgtc tttttctcct tttgttgttg gtgcatccct
      601 gttgctcggg gggccaatag cttttgctac tcctctttcg ggtactcaag aacttcattt
      661 ttcagaggac aattatgaaa aattattaac acctgttgat ggactttctc ccttgggagc
      721 tggtgaagat ggaatggatg cgtggtatat aacttcttcc aacccctctc atgcatctag
      781 aactaagcta cggattaact ctgatattat gattagcgca ggtcatggtg gtgctggtga
      841 taataatgat ggtaatagtt gtggcggtaa tggtggtgac tctattaccg gatctgactt
      901 gtctataatc aatcaaggca tgattcttgg tggtagcggc ggtagcggtg ctgaccataa
      961 cggtgatggt ggtgaggctg ttacaggaga caatctgttt ataataaatg gagaaattat
     1021 ttcaggtgga catggtggcg atagttatag tgatagtgat ggggggaatg gaggtgatgc
     1081 cgtcacagga gtcaatttac ccataatcaa caaagggact atttccggtg gtaatggagg
     1141 taacaattat ggtgagggtg atggcggtaa tggaggtgat gccatcacag gaagcagcct
     1201 ctctgtaatc aataagggca cgttcgctgg aggcaacgga ggtgctgctt acggttatgg
     1261 ttatgatggc tacggtggta atgctatcac aggagataac ctgtctgtaa tcaacaatgg
     1321 agctatttta ggcggtaatg gtggacattg gggggatgct ataaatggta gcaatatgac
     1381 cattgctaat agcggatata taatttcagg taaagaagat gatggaacac aaaatgtagc
     1441 aggtaatgct atccacatca ctggtggaaa caattcatta atactccatg aaggttctgt
     1501 cattactggt gatgtacagg ttaacaattc atccattctg aaaattatca acaatgatta
     1561 cactgggacc acaccaacta ttgaaggtga tttatgtgct ggtgattgta caactgtttc
     1621 actatcaggt aacaaattca ctgtttcagg tgacgtttct tttggtgaga acagttcttt
     1681 aaatttagct ggaatcagta gtctggaagc ttctggaaat atgtcatttg gcaacaatgt
     1741 aaaagtggag gctattataa ataactgggc gcagaaggac tataaactgc taagtgcaga
     1801 taaagggata acaggtttca gtgtttctaa tatatctatc atcaatccgt tactcactac
     1861 tggtgctatt gactatacaa aaagctatat cagtgaccag aataaattga tctacggttt
     1921 gagctggaat gatacagatg gcgacagtca tggagagttc aatctgaaag aaaacgctga
     1981 acttactgtt agtactattc tggcagataa tctcagccat cataatataa atagctggga
     2041 cggaaaatcc ctaacaaaat caggggaggg aactctcatt ttggcggaaa aaaataccta
     2101 ctctggtttc accaacatca atgcaggcat tctaaaaatg gggacagttg aagctatgac
     2161 acgtaccgct ggtgttattg ttaataaagg tgctaccttg aatttttcag gcatgaacca
     2221 aactgttaac actttattaa atagtgggac tgtgctaatc aataatatta atgccccttt
     2281 tttgcctgac cccgtcattg tcacaggtaa catgactctg gagaaaaacg gtcatgttat
     2341 tctcaataat agttcgtcaa atgtcggtca gacctatgtt cagaaaggta attggcatgg
     2401 aaagggcgga atattatctt tgggcgcggt tctcggcaat gacaacagta aaactgaccg
     2461 gctggaaatt gcaggccatg cgtctggtat tacctatgtt gcagtgacaa atgagggagg
     2521 ctctggagat aaaactcttg aaggtgttca aattatttcg acagattctt ctgataagaa
     2581 tgcttttatt cagaaaggcc gtattgttgc tggtagttat gactatcgcc tgaaacaggg
     2641 cactgtatct ggactgaata ccaataagtg gtatctaact agtcagatgg ataatcaaga
     2701 atcaaaacag atgagcaatc aagagtctac tcaaatgagt agtcgccgag ctagttcaca
     2761 gcttgtatct tcacttaatt tgggtgaagg tagtattcac acatggcgcc ctgaagctgg
     2821 cagttatatt gctaacctga tagcaatgaa cacgatgttt agtccttctc tctatgaccg
     2881 acacggtagc actattgttg atcctactac aggtcagctc agcgaaacca ccatgtggat
     2941 tcgtactgtt ggtggacata atgagcataa tttagctgat agacaattaa aaaccacagc
     3001 taacaggatg gtttatcaga ttggtggaga tattttgaag acaaacttca ctgatcatga
     3061 tggcttgcat gtgggtatta tgggagctta tggatatcag gatagcaaaa ctcataataa
     3121 gtatactagt tatagttcac gaggaactgt gagcggttat actgccggtt tgtacagttc
     3181 ttggtttcag gatgaaaaag aacgaacagg tctatatatg gatgcttggt tgcagtacag
     3241 ttggtttaat aatacagtca aaggagatgg gttaactggt gagaaatatt ccagcaaagg
     3301 aataacagga gctttggaag ctggctatat ctacccaacc atacgctgga ctgctcataa
     3361 taatattgac aacgcattgt atctcaatcc acaagtccag ataactaggc atggggtaaa
     3421 agcaaacgac tatattgaac acaatggcac tatggtcaca tcctctgggg gcaataatat
     3481 tcaagcaaaa ttgggattgc gtacatcctt aattagtcag agttgtatcg ataaggagac
     3541 tcttcgtaag ttcgaaccat ttttggaagt gaattggaaa tggagctcaa agcaatatgg
     3601 tgtaattatg aatggcatgt caaatcacca gataggcaac cgtaatgtga ttgaactcaa
     3661 aactggtgtg ggggggcgtc ttgcagataa cctaagcatc tggggaaacg tatctcagca
     3721 attgggtaat aacagttaca gagacaccca aggtattttg ggtgtgaaat ataccttctg
     3781 ataataaaaa tgcagggcgt gttgatgtcc tgcatttctc tgggacccac cgtgtaaaag
     3841 ctcggtgggt ttttgtgcac atgatggtat gcctgcgatc aatgccaaac gtgtttacca
     3901 gatcatgcgc cagaatgcgc tgttgcttgg cgttacacag gaattgcatt agaaagcgat
     3961 gaacgaatgc taccgccaaa tattcgtggt tatgcgcctc agattactgg tattgcagaa
     4021 actaatgcac gtgttgttgt atctcaacaa ggtcgagtaa tatatgactc tactgtacca
     4081 gctggaactt tttctattca ggacttgagt agttctgtac gaggaatatt ggatgttgaa
     4141 atttttgagc aaaatggtaa aagaaaacat ttccaagtag aaatgtgcag gtgtgccttt
     4201 cttatccaga cttggtcaga gtaggtataa attagtaaca ggtctaccta aaacaaataa
     4261 taaagcaact ggtgatgcat ttttttcggt gaagatccta cggggtcctg agcccggtga
     4321 agtggcgagg caaatcacat ggggaagcgt acagcctg
//
LOCUS       DAZOZX010000218         4209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZOZX010000218 DAZOZX010000000
VERSION     DAZOZX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.223"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUPT_003943"
     CDS             complement(<1..209)
                     /locus_tag="ACHUPT_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0821290.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHUPT_003944"
     tRNA            complement(324..399)
                     /locus_tag="ACHUPT_003944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHUPT_003945"
     tRNA            complement(406..480)
                     /locus_tag="ACHUPT_003945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHUPT_003946"
     tRNA            complement(597..681)
                     /locus_tag="ACHUPT_003946"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHUPT_003947"
     tRNA            complement(690..765)
                     /locus_tag="ACHUPT_003947"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUPT_003948"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUPT_003948"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0821291.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUPT_003949"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUPT_003949"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0821292.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUPT_003950"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUPT_003950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0821293.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZOZX010000219         4198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZOZX010000219 DAZOZX010000000
VERSION     DAZOZX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.224"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHUPT_003951"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHUPT_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR0821294.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHUPT_003952"
     CDS             complement(995..1690)
                     /locus_tag="ACHUPT_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0821295.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHUPT_003953"
     CDS             complement(1683..3110)
                     /locus_tag="ACHUPT_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0821296.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHUPT_003954"
     CDS             complement(3121..3840)
                     /locus_tag="ACHUPT_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0821297.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZOZX010000220         4186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZOZX010000220 DAZOZX010000000
VERSION     DAZOZX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.225"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..193
                     /locus_tag="ACHUPT_003955"
     CDS             <1..193
                     /locus_tag="ACHUPT_003955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821298.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(351..998)
                     /gene="ychE"
                     /locus_tag="ACHUPT_003956"
     CDS             complement(351..998)
                     /gene="ychE"
                     /locus_tag="ACHUPT_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0821299.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1029..1082)
                     /gene="ychE"
                     /locus_tag="ACHUPT_003957"
     CDS             complement(1029..1082)
                     /gene="ychE"
                     /locus_tag="ACHUPT_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR0821300.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1475..4150
                     /gene="adhE"
                     /locus_tag="ACHUPT_003958"
                     /gene_synonym="adhC"
     CDS             1475..4150
                     /gene="adhE"
                     /locus_tag="ACHUPT_003958"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0821301.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          960 g         1036 t
ORIGIN      
        1 ttcaattcaa tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag
       61 tagtgaaatg cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg
      121 cagtggaatt actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc
      181 gaaaataaca tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt
      241 gagaagaata tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa
      301 ataatgacga agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc
      361 aggccgggga aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc
      421 aatagcccca taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc
      481 cacggtgcca tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag
      541 aaaccaaaca gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc
      601 gcccctggcc ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc
      661 gcggtttctg atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc
      721 gacatcgcta tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata
      781 ccaaaaagtt gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca
      841 gacaggttgg ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg
      901 ctgataaaga cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat
      961 ttgaagtaaa cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc
     1021 ggataatgtt agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc
     1081 atttttcacc tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca
     1141 aaacatgcta atgtagccac caaatcatac tacaatttat taactgttag ctataatggc
     1201 gaaaagcgat gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt
     1261 acccagaagt gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact
     1321 ctaatgttta aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt
     1381 ttcatcggtt aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt
     1441 tactaaaaaa gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact
     1501 taacgcactc gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga
     1561 gcaagtagac aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact
     1621 cgcgaaaatg gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa
     1681 ccactttgct tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct
     1741 gtctgaagac gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg
     1801 tatcgttccg accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa
     1861 gacccgtaac gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc
     1921 ggctgatatc gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg
     1981 gatcgatcaa ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct
     2041 gatcctcgcg actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc
     2101 tatcggtgta ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg
     2161 tgcagttgca tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga
     2221 acagtctgtt gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca
     2281 cggcggctat ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa
     2341 tggtgcgctg aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg
     2401 cttctctgta ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag
     2461 cgaaccgttc gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt
     2521 cgaagacgcg gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc
     2581 ttgcctgtac actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat
     2641 gaaaacagct cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta
     2701 taacttcaaa ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat
     2761 ctctgaaaac gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga
     2821 aaacatgttg tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat
     2881 cgcgctggat gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt
     2941 cctgttcaac aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga
     3001 aactgaagtc ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc
     3061 agaactggca aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat
     3121 ggacgccgcg aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct
     3181 ggcgctgcgc tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa
     3241 agcgaaaatg atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc
     3301 ggttgtaact gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc
     3361 ggatatggcg attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt
     3421 cggtggtctg gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga
     3481 gttctctgat ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc
     3541 ctaccacgaa gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat
     3601 cgcgggtatc gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact
     3661 gggttcccag ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat
     3721 tcgctacaat gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc
     3781 gcaggctcgc cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga
     3841 ccgtactgct gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact
     3901 gggtattccg aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt
     3961 ggataaactg tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc
     4021 gctgatctcc gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga
     4081 agatgaaact gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa
     4141 atccgcttaa tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DAZOZX010000221         4182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZOZX010000221 DAZOZX010000000
VERSION     DAZOZX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.226"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHUPT_003959"
     CDS             complement(<1..186)
                     /locus_tag="ACHUPT_003959"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0821302.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUPT_003960"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUPT_003960"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0821303.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUPT_003961"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUPT_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0821304.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHUPT_003962"
     CDS             3863..4144
                     /locus_tag="ACHUPT_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0821305.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZOZX010000222         4168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZOZX010000222 DAZOZX010000000
VERSION     DAZOZX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.227"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUPT_003963"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUPT_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR0821306.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHUPT_003964"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHUPT_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHUPT_003965"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHUPT_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACHUPT_003966"
     CDS             3787..>4168
                     /locus_tag="ACHUPT_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR0821307.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZOZX010000223         4116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZOZX010000223 DAZOZX010000000
VERSION     DAZOZX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.228"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(28..285)
                     /locus_tag="ACHUPT_003967"
     CDS             complement(28..285)
                     /locus_tag="ACHUPT_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821308.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            741..1892
                     /locus_tag="ACHUPT_003968"
                     /pseudo
     CDS             741..1892
                     /locus_tag="ACHUPT_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            complement(1922..2350)
                     /locus_tag="ACHUPT_003969"
                     /pseudo
     CDS             complement(1922..2350)
                     /locus_tag="ACHUPT_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2865..3908)
                     /locus_tag="ACHUPT_003970"
     CDS             complement(2865..3908)
                     /locus_tag="ACHUPT_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0821309.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         1150 a          898 c          951 g         1117 t
ORIGIN      
        1 gtagatacat acgcagtcag agtacactta ctttttatta tttcctgcaa tggcgattac
       61 ggctattgac agtaatgaag ttattagtga accaagacct aaagtagttc cgggaccaac
      121 accgttgtcg cttacggtta ctgacaggaa actgattgta ccagcgctta ttgtttgagt
      181 aaaacctgta atagatgcgg caaatcctgt ttttgaacca tttatttgta atgcgataaa
      241 attggctatt gggtaataaa aaccataggt tggataattt ttcaatcttt gataattgat
      301 ttccgattat gaaaaataaa actgagaagg agaaaataat tgagtaacta aaagtactca
      361 ggccataagt atgcatgaaa attgatggtg atattgatat gtagcaaaat agagaactga
      421 gtattaatga gacaagtata gtgccgcaga taaactcttt ggtcacaaag gatgcactgt
      481 tttgacatat tactttctca tttgcatgag ttatctttgg tgataataac attattataa
      541 tactgaacag catcataaca agaaatataa cattccagtt ataatattca ataatcaatg
      601 cgcgaggtgc tactaatggt attactgcgc ttacagatga tatatcagaa agcgcccgtt
      661 ttagttttaa tggagggtga tcttacccac caatagtgga cacaggacta agtgagtaaa
      721 ctctcaacca gaggtgactc atgacaaaac cagtatcaat cagcaagaag ccccgtaaac
      781 aacatacgcc tgaatttcgt aacgaagccc tgaaactcgc tgaacgcatc ggtgtggccg
      841 ccgcagcccg tgaactcagc ctgtatgaat ctcagcttta tgcctggcgc agtaaacagc
      901 agcaacaaat gagttcgtca gagcgcgaaa gcgaactggc cgctgaaaat gtccgcctta
      961 aacgacaact ggcggagcag gctgaggaac tgtccatcct ccaaaaggcc gcgacatact
     1021 tcgcgaagcg cctgaaatga agtatgtctt tatcgaaaat catcgggcag agttcagcat
     1081 caaagcgatg tgtcgtgtac ttcgggttgc ccgcagcggc tggtatgtct ggctcaggcg
     1141 tcgtcaccag atgagcctgc gccaacagtt tcggctcacc tgcgatgccg ctgtccataa
     1201 ggcattcttt gaggcaaagc agcgatacgg tgctccccgc cttgctgacg aactgccgga
     1261 gttcaatatt aaaaccattg ccgccagcct gcgtcgtcag gggctgcggg cgaaagccag
     1321 ccggaagttc agcccggtca gctaccgtgc acatggcctg cccgtattgg agaatctgct
     1381 ggagcaggac ttcagcgcca gtggcccgaa accagaagtg ggcgggtgac atcacgtact
     1441 tgcgtaccga tgagggctgg ttgtatctcg cagtagtcat cgacctgtgg tcacgcgccg
     1501 tcattggctg gtcgatgtca ccgcgaatga cagcacaact ggcctgtgat gcactgcaaa
     1561 tggcgttgtg gcggagaaga cgcccggaaa gcgtcattgt tcatacggac cgtggtggtc
     1621 aatactgttc aggggattat caggcaatgc tgaagcgaca caacctgcgt ggcagtatga
     1681 gtgcgaaagg ctactgttat gacaatgcct gtgtggaaag cttctttcat tcgctgaagg
     1741 tggaatgtat ccacggggaa cgctttagca gccgggaaat aatgcgggca acggtgttta
     1801 attatatcga gtgtgattac aatcgctggc gtcgtcacag tgcctgtggc ggtctcagcc
     1861 cggaacaatt tgaaaaccat aatctcgctt agggccgtgt ccacattacg tgggtaggat
     1921 cagtatgggt cgtcttgtat ttgtcatact gcccaatctc accaatcgaa aatgctcctt
     1981 ttggcaaagg ttcaagcagg tctctgggaa tggaggtcgc tggcgtcgga tataaaaaac
     2041 cctcacggct tagcaaggct ggcgtcacta tcggtgtaac aatatcaggc gtgcctgcgg
     2101 aacggtcctt accatgctgc agtaaccgga aataaacttt tgggcgtaag atgacattga
     2161 cagtttcgac atcgtctttt tctcccttaa aaaatttatt gacaatcgct tcgtcaagac
     2221 gtcccgccgc tatcccatgc cagtgagtga aattttgggc gtctttccgt tcaaaacagc
     2281 cctttcctga ctcagcatct gcaagcgagt tgcgccagta tgaggcaaac cttaaagcat
     2341 tttcatccat taccagtcct gtgactcaat gaaatccatt gatttatcga atataaaacc
     2401 accgtatgga caactattgt ccatattaaa aaagttggat gcaatgtttt gtttataatt
     2461 cgatatcaaa tgtactggtt gtacgaggtt cagaacacaa gcacattcaa cagtacgtca
     2521 ggactattga tttcataaca gcgaatacaa ttttttactg tttataatgg ctgcctgtgt
     2581 tggacgggga taccagttca accgaaaacg ccagaggagg ggattacccg ctggcagggt
     2641 aaatctgtgg tttttgatgc cagctataac gtgtttgacg ataaagtatt acgaaagaac
     2701 tttaatgagt tcatggagta attcgttaaa gtaatttaca acgctatcct tttgcaaagg
     2761 aaactttcag aaaataaccc cagggtaatc acctggggaa ggaacacagt gttattattg
     2821 gataataaat tattacttgt tttaaagtta cactaatgca atatctactg atactggatg
     2881 cttgaatgtt tcatccctgc tgcggaaaag ggcaatatcc agggccagat atattcaccg
     2941 ttcatatttc tgcatttctg tacaatttcc tcagaaagtg tcttgtcaat tcttttccac
     3001 aggtgctcct gctgtcttaa aatactttcg catatttcga aatcatcttc ctgaatcagc
     3061 atttcggcgc atttaatgat ttctgatcgt tcatgcagca cggaggaatg gttatagagc
     3121 cagtttacaa acagcaatgc ttccggatat tgtccccata atggtgagta tgttgcatag
     3181 taatcttctg ccgtatccag agagccggag aagtcatgcc acacagcaag tggaaatgga
     3241 tgcagatttc cgcacagcat ttctgcgatg taagtgtttg tggcaaagcc atggcgaaaa
     3301 gctgtggcgg ccttaacatg ttccccgttc agaaaatgca ggagaccgag ttcataccag
     3361 taaggtgaaa attcatcagc atgctctttc agtacactga aggctcgctc atgatcgccg
     3421 gtgcgtaaca gctctgaacc caacaaccag cgagccccct gattatcttc aggattgtat
     3481 gccagtattt tatcagtgag cataaccgca tcctgatggc gctgtaagtg cacatttgcg
     3541 agaattgctg cataaagcgc gcgcagataa ggccggtttt cgggatgcat ccagattatt
     3601 tcaccacaga aactttccgg gatgatacga ttacctgccg ccagtccttt aagagcggcg
     3661 ttcagtgcct tacgcggggc attctgttcc agaaatgcat atgccagatg agcgtgtata
     3721 tcaataaagt ccggctcccg ttgagcaagg cgtcggagtt tgttgatgta actcgtctcg
     3781 ctgatttcat cagcgtcgta tgcatcaagc acttcttcaa actcccccag caatgggcca
     3841 aaccaggtat ccggaaatac gaaataaccc ttgttatcgt gcacttcaaa gcataatcta
     3901 tcgttcattt tctgagcctg taaaacatgt ggtgatatag tctgagagca taaaagttga
     3961 gattctgtgt atcaagtcct gtcttggcac cgaatgaata ttgcttacga agccccttca
     4021 tgctttcctt gagaacaata attttatctt tatgtgaatc tcatttttta taatgatgtc
     4081 agatgattga aatgctaaat tatatctgaa tcaact
//
LOCUS       DAZOZX010000224         4114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZOZX010000224 DAZOZX010000000
VERSION     DAZOZX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.229"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..158)
                     /locus_tag="ACHUPT_003971"
     CDS             complement(<1..158)
                     /locus_tag="ACHUPT_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR0821310.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHUPT_003972"
     CDS             complement(308..469)
                     /locus_tag="ACHUPT_003972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821311.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHUPT_003973"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHUPT_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..1249
                     /locus_tag="ACHUPT_003974"
     CDS             914..1249
                     /locus_tag="ACHUPT_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0821312.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="ACHUPT_003975"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="ACHUPT_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="ACHUPT_003976"
     tRNA            complement(2161..2237)
                     /locus_tag="ACHUPT_003976"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="ACHUPT_003977"
     tRNA            complement(2339..2414)
                     /locus_tag="ACHUPT_003977"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="ACHUPT_003978"
     CDS             complement(2559..3380)
                     /locus_tag="ACHUPT_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0821313.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="ACHUPT_003979"
     CDS             complement(3395..3751)
                     /locus_tag="ACHUPT_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0821314.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="ACHUPT_003980"
     CDS             complement(3764..>4114)
                     /locus_tag="ACHUPT_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0821315.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAZOZX010000225         4113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZOZX010000225 DAZOZX010000000
VERSION     DAZOZX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.230"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            375..578
                     /locus_tag="ACHUPT_003981"
                     /pseudo
     CDS             375..578
                     /locus_tag="ACHUPT_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            597..3983
                     /locus_tag="ACHUPT_003982"
     CDS             597..3983
                     /locus_tag="ACHUPT_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0821316.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VGSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
BASE COUNT         1022 a          938 c         1278 g          875 t
ORIGIN      
        1 gttgttaata aaaagcttat agatttatag ttgataatta aaacgcatcc gatatttcag
       61 ctttttctat gcaagataca attttataag gtgtccattt ttatatggag gcggtagcgt
      121 gtctgttact agtggtttta tttgcatagt gaatatattg ggattgttaa atacataact
      181 atgtatccgc tctggtcgtt gtaaatattg ctaattgctt aatattatta gtgattatga
      241 ctggaaagat aaagtgtgct gtaaagcggt gatgggtatt acattcatga ttaaaaaaac
      301 agaacctgtc aataaataat tcctttatta aaacggacta tttacattat gacggaatat
      361 tatctcaacg aggcggtttg gaaatacaga gccagtttct ccggggtaac gagcaggtgt
      421 accagtgtca ggtggcattt gtattgccgg gtgagcgggt catgatggcc ttcacctacg
      481 ccagaaccac accgctgacc ccggcagata tgacgcgctg ggctgaaatt aaaaagaatc
      541 tgcgcttcag gatgcggcag gaagtcagga cgaattaatc atacagggaa cgggacatgg
      601 aagaggcatt ctgggcggca agacagggtg atgccctgct gcatacgtcg tttatggcag
      661 atgtgctggg ggcggtgctg gaggtggccg caaatgtcgt tattgatgcc ctcattgtcg
      721 gcacaacctc gttactggcg acgtcagtgg ggatcacgat ggggtgcacg gccgtcgtgc
      781 tgaccggttt tgtggcgggt gtggcgatgg tgtacaccgg cgtgacagaa aaggtcagcg
      841 aagcctgttc agccctcgca aatatgcttt tcccgcctca gattgaaggt tacatcctca
      901 ccggctctgg tgacacatgg atcaacagca aacctgccgc gcgggcagcg gcgactgcgg
      961 catcgcggca ggacatagag gcgcaggaag cgcaggcgaa ggcagaacag gaagaagcgc
     1021 agcgtcagga ggatgccaga acgttccggg atgtggccgg ggaatatctg gaaatggcgg
     1081 gggccatagc cctggcggtt aacccggtga cagggcctgt gctgatgtcc cgggctctgc
     1141 acgaccagct cagtacggaa gaagggcgtt ctgaaatggt ggatggcgtg acacattttg
     1201 tgtcggaact atggcagccc actgtggcca gtgcggcccc gggatccacc ccgacccctg
     1261 acgataaaat cgactgccat aaacatcctt cctcactgac gcagtttctc gcacaaaaaa
     1321 aagccgcctt tctggatgcc cccctgggaa cggcccttaa cctgctgaat ccgggcggta
     1381 tgctggaaac cgcctttcag ggagtgaatg cggttattgg cagcatcagt aatctgttca
     1441 aaggcgatga cgagccgccc gcagcggaat atatcgcgga aggcgcgcgg gatgtgcgta
     1501 tcaacagcca gccggcgaca cgcagcgggg cacgctgtac ctgcgaggcc agggtggtga
     1561 atgagccggg gaacggggcg tttgtgtcac cggatgtgcg cattggcggt ccgttactgg
     1621 tggtcaggga tatcagaagc ggcaggtcgc agataaccct ggtggcaacc gtggcgctga
     1681 tgtttcttcg cccgggcaaa atgctttcta aaatcgcctg ttttgctgcc agtgtcggga
     1741 tgagcatgct gacgcagaag gccaccagtg cccttccgca tccggtcaat gccgccaccg
     1801 gggccaaata cctggccgat gacgatgact tcgatttcag tctgccgggc cactttccgc
     1861 tggactggca gcgcgtgtac agcagtcgtg acgaacgtac ggaaggaatg tttggtcagg
     1921 gctggagtgt aatgtatgaa gtcagcctcg tgtgcacacc gggtagcgca gacgaaaact
     1981 gcatgacctt tgtgtcgggc atggggcgac gcttggatat ggaagccgta ctgccggggg
     2041 gcgggtttta cagccccggt gaagggctgg cggtaaggcg tggtgagcag gggcactggc
     2101 ttatcagcag cgatgacggg cagtgttttc tgtttgaggc tgacccgcac cacccgcagc
     2161 gccagcggct gaaaatgctg ggcgaccgta acagcaactg tctgaatctg tactacgacg
     2221 accggggccg cattgtggaa atcagcggcg aacagcagcg tccgtgcatc cgtctgtatt
     2281 acgaactggc ggcgcatccc cggcgggtga cacagattta tcagcatttc cctgagactg
     2341 cacctttact gctgcgtcgt tacagctatg acgaggccgg ccacctgaac ggcgtgtacg
     2401 acagcacggg gcacctgctg cgtgagtttg cttacgacga aaaccactgc atgacgctgc
     2461 accgccagcc gggcggcgaa gggtattatt accagtgggg ctggtatgaa ggtccggatg
     2521 acgcggcatg gcgggtgacg gggcaccaca cggacagcgg tgaacagtac cggctggcgt
     2581 gggacctggc gcagcgcagg ctgtgcgtga cggacggact ggggcgcaca cgttatcacc
     2641 agtgggatgc gcagaatcag gtgacggcgt accaggacga agccggacag gtgacgacat
     2701 tccgctggag cgacgaagaa cgactgttgc tggggatgac cgaccctcag ggcggcaaat
     2761 ggcgctatgt gtatgacctg caggggcata tcacggagac gcacgacccg ctggggcggg
     2821 tggagcaggc gcagtggcac ccggtgtggc accagccgga aacggaggtg gacgcggcgg
     2881 gaaacctgct ggggaggcgc gcgggggaaa gggccacggc agaaaacagc gtggtgccgt
     2941 tcaaccggct gatgtcttac cgtggtgtgc actaccgtta tgacgaattt ggccgtgcgg
     3001 tggaaaagga gggccggagc ggaacgcaga gttaccgtta tgatgcggaa caccggatgg
     3061 tggaagtgac gacagcgcgg gggacatacc ggtatgtgta cgatgcactg gggaggcgga
     3121 cggaaaaaca gcacatcagc cctgacggta agccgtacaa ccggacaaaa tttttatggg
     3181 acggaatgcg gctggcgcag gagagcaggc cggagggaac aggcagcctg tacatctaca
     3241 gggaccaggg gagttatgaa ccgctggcgc gggtggataa ggcgggcaaa gagggcccga
     3301 accggatact gtattttcac acggatgtga acggcgcgcc ggaagagatg acggacagcg
     3361 acgggaaaat cgtgtgggag acggggtatc aggtctgggg taacacgata caggagaagg
     3421 accacggcgg ggtggagcag aacctgcgct accagggaca gtacctcgac agggaaacgg
     3481 ggctgcacta caatctgcac aggtactatg atccggatgt cgggcggttc atggtaactg
     3541 acccgatagg gttgagaggc ggtttaaatc tctacagtta tgcacctaat cctctaaaat
     3601 atgctgatcc acttggatta actccatgtg ctgttagtaa ccaaaaagcg aatcgtttat
     3661 tagatagtag tgaaaccaaa gttactgttg ggtctagaag tgacgctgaa caattgttta
     3721 tggatagata tttaggtcat aattataaaa atatgacagg tgagtcaggc cccagcacta
     3781 agaacttaat ggaatatctt actgaaaata aaacaaaagc aggttcatat cattgggatg
     3841 atataaaaga tccttctgta acaaaacctt catatagagt gtcaggtcat gggccaggaa
     3901 atcctgatgg tgatttacca catttacaag ttcatcaaca tggtggaagt gttagacata
     3961 tatttttccc gtgggagaca taataatgaa caatctcaat acattattaa gtggaaaggt
     4021 tttgctcaaa gaaattagtc cattaatgcc tggatataga acatggattg aaatttcatt
     4081 gatcgatgaa ttaaaaccat cttatccttt tag
//
LOCUS       DAZOZX010000226         4057 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZOZX010000226 DAZOZX010000000
VERSION     DAZOZX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.231"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUPT_003983"
     CDS             complement(139..3654)
                     /locus_tag="ACHUPT_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0821317.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1049 a         1027 c          843 g         1138 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaa
//
LOCUS       DAZOZX010000227         4054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZOZX010000227 DAZOZX010000000
VERSION     DAZOZX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.232"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /gene="bglB"
                     /locus_tag="ACHUPT_003984"
     CDS             complement(<1..1025)
                     /gene="bglB"
                     /locus_tag="ACHUPT_003984"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0821318.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1044..2921)
                     /gene="bglF"
                     /locus_tag="ACHUPT_003985"
     CDS             complement(1044..2921)
                     /gene="bglF"
                     /locus_tag="ACHUPT_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0821319.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3055..3876)
                     /gene="bglG"
                     /locus_tag="ACHUPT_003986"
     CDS             complement(3055..3876)
                     /gene="bglG"
                     /locus_tag="ACHUPT_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0821320.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT         1081 a         1061 c          888 g         1024 t
ORIGIN      
        1 aatccaaccg gatcaatttg ccacccccac tctgaacttt tcagatgcgg attggggatc
       61 atattcagta tgttgccctg cgcattttta ttaatgcttt cgtcgtggga aacacaacca
      121 gtcatgtaat aactaaaaga gatgaaatct acagtatgtt ttaaatcttc tgcgtcactt
      181 tcagtcatct caatggtgat attgtggtcg cggaagaaac gctgcatata gccgggatat
      241 tggccacgcg cctgaacatc accaaagaac atccagcgcc ggttctcttc catggcctgc
      301 aacatatcct gtggctggca ggtgaggggg taaaccagcc caccgagaag catattgccg
      361 attttcgctt cggggatcag gctatgatag gctttaactg cccgcgcact ggcaaccagt
      421 tgatgatgga tcgcctgata aacttccgcc tcgccactct cttctgccag ccccacgccc
      481 gtgaatggcg cgtgtaacga catattgatt tcattaaacg tcagccataa cgctacttta
      541 tgttggtagc gggtaaagac cgtgcgggcg taatgctcga agtgatcgat gaccgcccga
      601 ttagcccaac cgccgtagtt tttcaccagc ccatatagca tttcgtaatg ggataacgtt
      661 accagcggct tgatccccgc ctgcgccatt tcatcaaaca gccgatcgta aaacgctaac
      721 cccgcttcat tcggttcggc ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa
      781 atacgcagac aggtgaagcc catctcggca aataacgcga tatcttccgg gtaacggtaa
      841 taaaaatcga tggcgacatc tttgatattc tctttcccca ggatgcgcgg ttccattttt
      901 cccattacgc catgaggctg taaatctgag gtcgtgatcc ctttgccatc ttccagccag
      961 gcaccttcca cctgattggc agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct
     1021 ttcataatta actcctttta tcgttagcga atgatggata acagcggttc acctgcgctt
     1081 atctgcgccg tgccgtgggg taatacgtct gtaaaatcat cgctattact gattaatacc
     1141 ggcgtcgtca gatcaaatcc ggcctcgcga atagcaggga tatcaaaaga aatcagccga
     1201 tcgcctgtat tgaccttgtc acccacgttg acgtgagcgg aaaagaattt gccgtccagt
     1261 tttacggtgt cgataccgac atgaatcagg atctccacac catcatctga ctcaatgcca
     1321 atggcgtgta atgtggcgaa caacgaagca attcgacccg caaccggaga acgcacttca
     1381 ccaaccgagg gcagaatggc gataccttta cccaacaggc cactggcaaa cgtggtatca
     1441 gcgacgtgaa tgagcggcac aatctctccc gtcatcggtg aacagatacc gccctgctca
     1501 ggtggtgtaa taacctctgg tgttttctct tgcggggcac cctgcgctgg ctgacgttta
     1561 ctggtgatga aatgaagcat caacgtacca acaaatgcgc aacctatggc aatgacaccg
     1621 ccaataacgc tggcccagac ggtgaaatca atccccgttg acgggatggt ctgcatgaag
     1681 gtgaaaatac ttggcaaacc aaaggagtaa actttcgttt gcgcgtagcc aatgatggtg
     1741 gcccccaatg ctccactgat acaggcgata acaaaggggt acttacgcgg caggttgacg
     1801 ccatataccg ctggttcggt gataccaaac agacccgtca acgccgctga tcccgccacc
     1861 acttttttct gcgcatcgcg ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc
     1921 ataatggcgg gcattaacag cgggatcatg gtgtcgtagc ccagcacggt gaagttattg
     1981 atacacagcg gcaccaggcc cctgtgcagt ccgaacatga cgaagatttg ccagaagccg
     2041 cccattaccg cgcccgcaaa tgcaggaacc gcctgataaa gccagagata accggcggca
     2101 atcatttcgc ttatccaggt tgatagctgc cccaccagca gaaaggtgac gggtgtgata
     2161 accatcagac atagcaatgg tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta
     2221 agtcggcgtt ccagaatgct gcacaaccag gcagaaaaaa taatgggaat aaccgatgac
     2281 gagtaattca acaatgtgac cggaataccc aggaaatcca gccccggcgc atccgctttt
     2341 tgcccgttct cgaaggcagt cagaattaat ggatgcacta acgctccacc aatcaccatg
     2401 gcagtaaatg gattaccgcc gaagcgtttg cccgcggtgt atcccaggat tatcgggaag
     2461 aacccaaaca aggcatcact ggcgctgaat aaaattaaat aagtaccact ttgttcggtc
     2521 gtccactgaa aagtgagcgc cagagccagc atacctttca ggatcccggt tgccgccatc
     2581 agaccgatca gaggcgtaaa aatacctgaa ataacataaa caaagcggtt tagcagatta
     2641 cctttctcat cattttccgg tgcctgttgc gctttttcgc caaggcctgc cacgctgtta
     2701 accgccagga agacatcggc cacatggtta cctatgacca cctgaaactg accaccgctt
     2761 tccaccacca taataatacc gggggtcttt ttcagtacct cagcttgcgc tttgctttca
     2821 tcctttaact taaaacgtaa tcgcgttgcg caatgcatca gactcacaat gttatctgcg
     2881 cccccgactc ctgcgactat ttttctggct aactccgtca taacttgccc tctaccgctt
     2941 tgcggcaaaa ctccaaaaaa aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt
     3001 tttcaggttt tgcccgctta gtgcggtaac aatcctttac tcagtaataa tatttcagtg
     3061 ttctttgcgc acgcgctcta tatttatggc taaaaacata atctctgcgg gtgaaatttt
     3121 acgttgatac tgcaaaccaa taaaaatggc gatccgttcc gcacattgcc atgcttgcgg
     3181 gtaattttgt tttactgctt gttgtaatga ttcatcacta tcgttaattg aagcatgttc
     3241 aagaatacgc caggataaaa acttcagatg tgtaaccagt cgctgataac tcaagctttc
     3301 ctcctggtaa ttaaggctga actgaaattt tattaattgc agcatttcgc gcattaactg
     3361 cgtgacacct gcaacatcct ccatatttcc gctcatttgg gcactgacca gatgcatggc
     3421 aataaagccc acttcatctt tcggtaactg cacgcccaac cgtttatcaa taatggttaa
     3481 cgcttcttcc cctagctgga actctttcgg gtaaagccgc tggatatccc acagcaacac
     3541 gttttgctga aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga tataaatact
     3601 gtcctgtaat tttcccaagc gctcctgcgc taaagagata agacgatcac aggttgccat
     3661 cacctcaaga ggaatatgac ttaagagttc gcttaatcgc ccgttcagtt catgactgct
     3721 caaggcatac tctttttcta ttccacttga gttaattctt tcgccagcgc gtttttgaaa
     3781 gccaattccg cgccccatga cgactttttc ccgctgttga tcatcaataa ccaccacaac
     3841 attattgttg agaattttgg tgatttgcat gttcatagca aggacctttt ttataaacaa
     3901 aaaaaaaccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta
     3961 acaatccagt catttattaa tggtttttat aacgaacatc caggttcgga aattaattta
     4021 attgcgtgca gagaaaatag caatgcgcta ttga
//
LOCUS       DAZOZX010000228         4042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZOZX010000228 DAZOZX010000000
VERSION     DAZOZX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.233"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(126..284)
                     /locus_tag="ACHUPT_003987"
     CDS             complement(126..284)
                     /locus_tag="ACHUPT_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0821321.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHUPT_003988"
     CDS             complement(886..1077)
                     /locus_tag="ACHUPT_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0821322.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHUPT_003989"
     CDS             1142..1465
                     /locus_tag="ACHUPT_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0821323.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHUPT_003990"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHUPT_003990"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHUPT_003991"
     CDS             2143..2673
                     /locus_tag="ACHUPT_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0821324.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHUPT_003992"
     CDS             2680..2913
                     /locus_tag="ACHUPT_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0821325.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHUPT_003993"
     CDS             complement(3459..3851)
                     /locus_tag="ACHUPT_003993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821326.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZOZX010000229         3931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZOZX010000229 DAZOZX010000000
VERSION     DAZOZX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.234"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            72..302
                     /locus_tag="ACHUPT_003994"
     CDS             72..302
                     /locus_tag="ACHUPT_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821327.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            280..780
                     /locus_tag="ACHUPT_003995"
     CDS             280..780
                     /locus_tag="ACHUPT_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR0821328.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            780..2198
                     /locus_tag="ACHUPT_003996"
     CDS             780..2198
                     /locus_tag="ACHUPT_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR0821329.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            2198..3046
                     /locus_tag="ACHUPT_003997"
     CDS             2198..3046
                     /locus_tag="ACHUPT_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR0821330.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            3046..3501
                     /locus_tag="ACHUPT_003998"
     CDS             3046..3501
                     /locus_tag="ACHUPT_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR0821331.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            3504..>3931
                     /locus_tag="ACHUPT_003999"
     CDS             3504..>3931
                     /locus_tag="ACHUPT_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR0821332.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
BASE COUNT         1035 a          956 c         1013 g          927 t
ORIGIN      
        1 gttggtctgc ctaaccagac cgcgtgataa ccagagggag cttcggctcc cgtttttatc
       61 tggagacaag catgacacac atgatctttc gtcatggcga catgaagaag tggaaaggcg
      121 ttggctacga ctttgaaatc gtgaaagccg aagagcttca ggaatatctg gatgctggct
      181 ggttttcaca tcctgatgac cttttgaagg atgttgcaga gccagagcca gaagaaaaac
      241 agcgtaaaaa gcctggtcga aaacctaagg cggcagcaga tgaacctgac aacgaagggt
      301 gatttagttc ttgcggcatt acgtaagctc ggtgttgcat caaatgccac gttaaccgat
      361 gtcgaaccgc agtctatgga agacggcgtc aacgaccttg aaatgatgat ggctgaatgg
      421 cttggcggtg atgcgtcacc tggtatcaac gttggctaca tttttgctga tgcagatgtt
      481 gctccggatc cgggcgatga gcacggttta tcaaataacg ctatcaatgc cgtcattttc
      541 aaccttgcct gccgaattgc tccagattat gcgctggaag cgtctgcaaa acttataacc
      601 actgccagat acgggaaaga gcgactcgtc aaactgtctg caatggacag ggcaaaagca
      661 gctaaatgta agtccggtta tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg
      721 aagtggaatg gttggaatta cttccaccga aaggaacctt gcgataacgg gagcgaataa
      781 tgcctattca gcaacttccg cttatgaaag gtgtcggcaa agacttccga aacgccgact
      841 atatcgacta tctgccagtg aatatgctgg ctacacccaa agaaatcctg aacagcagcg
      901 gatatcttcg ctcattcccg ggcattgcca aacgttctga cgtgaacggt gtatctcgcg
      961 gcgtcgagta caacatggcg cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca
     1021 aaggagaaag tgaagtcggt gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc
     1081 ggacatcaca ggcggtaggc gttaatggtc aactggtcga gtatcgctat gatggcacgg
     1141 tcaaaaccgt ctcaaactgg cctacagaca gtggattcac tcagtatgag ttaggttcag
     1201 ttcgcgacat tacgcgctta cgtgggcgtt atgcgtggtc aaaagacgga actgattcat
     1261 ggtttatcac tgaccttgaa gacgaatctc atcctgaccg ttacagcgca caatatcgtg
     1321 ccgagtctca gcctgacggc atcataggta tcgggacatg gcgagacttc atcgtctgct
     1381 ttggttcatc gactattgaa tatttctccc tgactggcgc aaccaccgtt ggtgccgctt
     1441 tgtatgttgc acaaccatcg ctgatggtgc agaaaggcat tgccggaact tactgcaaaa
     1501 cgccattcgc tgattcgtat gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt
     1561 atatcatcga ctccggtcag gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc
     1621 gctcctacac tgctgatgaa ctggctgatg gtgtgatgga atcgctgcga tttgatgctc
     1681 atgagttgct gattatccac cttccgcgtc acgtcctcgt gtacgacgca tcttcaatcg
     1741 ccaatggccc acaatggtgt gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta
     1801 tcgacttcgt ttacgaaggc aatcagataa cgtgcggcga taagctggaa tctgttaccg
     1861 ggaaactgca attcgatatc agcagccagt acgacaagca acaggaacac ctgctgttta
     1921 ctccactctt caaagcggat aacgccagag tgttcgacct tgaggttgaa tcgtcaactg
     1981 gcgttgcgca gtacgccgac cgcctttttc tctctgcaac cactgactgc atcaattacg
     2041 ggcgtgagca gatgattgag cagaatgaac cgttcgttta cgacaagcgt gttttgtgga
     2101 agcgagtagg gcgcatcagg aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac
     2161 ctgtcactct gtctggcgct cagataagga ttgagtaatg gctgattcga atctcaatgt
     2221 gccggtaatc atccaagcta cgcggctcga tacatcagtc cttccacgca atatcttctc
     2281 gcagtcgtat ctgctttacg ttatcgcaca gggcactgat gttggtaacg tggctaacaa
     2341 agccaacgag gccggacagg gcgcttatga tgcacaggtc aggaacgatg agcaggatgt
     2401 gattctcgct gaccatgagc agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga
     2461 ggagcgaatc agacaggcag aatcaactct tcaggaccat gaaacacgaa tagctcagaa
     2521 tgaaagcgat atttcgtcgc ttgacacaag agttcagtcg ctggagtcgc aggtttcaga
     2581 ccatgaaacg cgcatcgatg ctctggagta tgcaaccaca cgcaagaagt cagaggttgt
     2641 ttactctggt gtatctgtaa ccatcccgac agcgccgacc aaccttgtta gcctgctgaa
     2701 aacgctcaca ccgtcatccg gctcgttggc accattcttc gacaccgtta acaacaagat
     2761 ggttgtgttc aacgagaaca aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc
     2821 cagcggaacc gccaacaggt caatgcagct aaccttttcc ggctctgttc ctgacacact
     2881 ggtcagcagt cgtaatgcgg cgacaacaac cgacaacatc ctgttagcta cgttcttcag
     2941 cgtggataaa gacggctttc ttgccacaaa tggcagtacg ttaaccattc agtcgaatgg
     3001 tgcggcgttt actgccacaa ccatcaagat aatcgcggag cagtgatgat tacattcaaa
     3061 ccaacgcgaa acatcgacct gatagaagca gtcggaaatc accctgacat tatcgccggg
     3121 agcaacaacg gtgatggata cgactacaaa cctgaatgcc gttactttga ggtgaacgtg
     3181 cacggtcagt ttggcggcat tgtttactat caggagattc agccgctgac attagattgc
     3241 cacgccatgt acctgccaga gattcgcggc ttcagcaagg aaattgggct ggcgttctgg
     3301 cgatacattc tgactaacac caccgttcag tgcgtaacat cgttcgctgc acgcaaattc
     3361 cgacacggtc agatgtactg cgcaatgatt ggccttaagc gtgtaggaac catcaagaaa
     3421 tacttcaaag gcgtggatga cgtgactttt tacagcgcca cacgcgaaga actaatcgac
     3481 ttcctgaatc acgggagata gccatgttat atgcatttaa gctgggcaga aaactgcgcg
     3541 gcgaggaacc ttattgccct gaaaaaggcg ggaaaggtgg cagttctgat aaaagcgcaa
     3601 agtatgcagc agaagcccag aagtatgctg cagacctgca aaatcagcag tggcagacga
     3661 tcatgaaaaa ccttgctccg ttcacgcctc ttgcggagca gtatgttaac cagcttcaga
     3721 acctttccag tttagaaggt caggggcagg cacttaatca gtattacaac tctaagcagt
     3781 ataaagacct tgcagggcag gcgcgttacc agagtcttgc tgctgcggag gcgacgggtg
     3841 gacttggttc gacagccaca agcaatcaac tggctacgat cgcgccgaca ctcggtcagt
     3901 cttggttatc aaatcagatg agcaattaca a
//
LOCUS       DAZOZX010000230         3883 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZOZX010000230 DAZOZX010000000
VERSION     DAZOZX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.235"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHUPT_004000"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHUPT_004000"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0821333.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACHUPT_004001"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACHUPT_004001"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0821334.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          984 a         1033 c          976 g          890 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gatttttaat cgt
//
LOCUS       DAZOZX010000231         3876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZOZX010000231 DAZOZX010000000
VERSION     DAZOZX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.236"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            69..763
                     /locus_tag="ACHUPT_004002"
     CDS             join(69..315,315..763)
                     /locus_tag="ACHUPT_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0821335.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(882..1280)
                     /locus_tag="ACHUPT_004003"
     CDS             complement(882..1280)
                     /locus_tag="ACHUPT_004003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821336.1"
                     /translation="MNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLH
                     PVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGTLQ
                     INTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1277..1690)
                     /locus_tag="ACHUPT_004004"
     CDS             complement(1277..1690)
                     /locus_tag="ACHUPT_004004"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFR0821337.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKN"
     gene            complement(1724..3184)
                     /gene="gadC"
                     /locus_tag="ACHUPT_004005"
     CDS             complement(1724..3184)
                     /gene="gadC"
                     /locus_tag="ACHUPT_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR0821338.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(3291..3876)
                     /locus_tag="ACHUPT_004006"
                     /pseudo
     CDS             complement(3291..3876)
                     /locus_tag="ACHUPT_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006663458.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT         1230 a          812 c          753 g         1081 t
ORIGIN      
        1 aatgctggat gggtgatgcc tctaattagt tgaatctgat gtataatacg ggcttttgag
       61 gttatctcat gaccagcgtt aatattcatt gtccccgttg tcagtcagct caggtttacc
      121 gccatggtca gaaccctaaa ggccgtgaca gacttcgctg ccgtgactgc caccgtgtgt
      181 ttcagttcac ttatacttat caagcacgta agccgggtat gaaagagctg ataactgaaa
      241 tggcctttaa tggtgccggg gttcgcgata ccgccaggac actgaaaatt ggtagtaaca
      301 ccgtcatccg gactttaaaa aactcgcgcc aaagcgaata acttcttcgc ctgttgccca
      361 tgctgatgtg gcgtttatct gcgagcttga tgagcaatgg agctacgttg gcagtaaagc
      421 ccggcaacat tggctcgggt acgcgtacaa caccaaaaca ggcggtgtac tggcctacac
      481 ttttggtccc cgaaccgatc aaacgtgccg ggagctactg gcactgctta cacccttcaa
      541 catcggcatg ctgaccagcg atgactgggg cagctatggc cgggaggtgc cgaagaataa
      601 gcatctgacc ggcaaaatat tcacccaacg cattgagcgt aataatctga cgctacgcac
      661 ccgcattaag cggttgggtc gtaaaacaat ctgcttctca cgctcagttg agatccacga
      721 aaaagtgatc ggagcgttta tcgaaaaaca catgttctac taattggatg cactacccag
      781 tatgtaactg ggtttgtcca taaaatgcac cttactcagt tggtgtccaa cttttggggt
      841 gcagttcaga aggacgggat tttacggtgt accggataaa tttaaaactg cctgatactg
      901 aatgtatgag gtttcgtaat gctcaattcc cctagtaccg ttttgtagat ctccatactt
      961 ccactagtat ttatttgcaa agttcctata tttgaggagt ttaataaatt tagctgtccc
     1021 aagtgatttt taatagagat agcctgatca tgtgcaggat taataaattt tatatccggt
     1081 gcaactgctt caaaaaaatt ctgaacaatt ggatagtgag tacagccgag aataatttca
     1141 tttactggat ggagggaaat taaatggtta acatgttttt tgatgtcatt caagattgca
     1201 ggagcattaa gattaccttc ctcaattaac attgcaagat ttttgcttgg ttctccaaat
     1261 acatttattg ttgaattcaa ttttttatta gctctttgtg acaccccgtt ttaatagtaa
     1321 actcggtagc gataatacca acatttttat attgctggcg aatcacatct tctactgcag
     1381 gttcaatgat ggataagata ggaaacggaa agctcttagc atatccgctg tattcttcgg
     1441 attccaaagt tgaggatata gtattacatg cgacagcaac tagtttgaca ttccttgact
     1501 gtaaaaaagt cagcattttt ttagtcaaac tgatgatatc atcggcagaa cggttgccat
     1561 aaggtgcatt catattatct ccacaataga taatatcttc accaggaagc aaattaatta
     1621 tttcctttac cgatgtcaat ccaccgacac ctgagtctat tacaccaatt ggagattcag
     1681 cactaaacat atttaacctc atcttttgta gcttatggaa aatttaatta ctagttcttt
     1741 tttcccaaga aaatggttcg aaatcagagt ttggatcttt ccagtcagat tttcggaagg
     1801 cgtaaattaa tattggaatt aataaaaata gggcaactaa tactaacaaa taaagaacat
     1861 agctggttgg actaccagtt gggatttgct cgggcggaat aaaacttaat acaaaagcca
     1921 gaaaagaccc taagaatcca agtgttgccc agaaaaataa agcaggggct cgatatgggc
     1981 gttttgtatc aggttgagaa tgtcgtagat agatagcact agcgaacatg agcatataca
     2041 taactaaata gagcataact gttaactgtg ataaaatctg aaaagctgct tgaactgaag
     2101 gcattatcac aaaagtaaca gaaagaactg agacaattat accttgtaca aacattagat
     2161 ggtgccccat tccatgacga ttggtttttt ggaaaaactt aggtaaataa ccagctttcg
     2221 cgatcgctaa tacacctgta ttaggccctg caatccaagt aaccacccca cccagtacgc
     2281 caattgctag cataaatgcc atcacagagc caagccatgg cactccagcc cattcgaaca
     2341 gcatatcgta ggcaaccaac aaactttgag ttagactaat tttatctgta ggaataataa
     2401 aggcgacccc taaagtactg agcaggaata ttgcaatagt acctatagaa gcgatggtaa
     2461 ttgcaattgg gtatttttta gttggattat ctaaatcgtt aacatggacc gcattcattt
     2521 ccatccctgc ataagcaaga aatatgctgg cggcaagaac aatattctgg aagttagcta
     2581 tattagggaa aagatcaccc caacctaatg ggatcaccgc tggtttaccg ctgaaccaca
     2641 gataggagaa accaagaata acaaggataa tagctggaat aattgtccct ataataccgc
     2701 accacttaga tacctgcgag aaaataccaa cgccacgtaa tgcaacacaa atagctcccc
     2761 agtaaacaaa tagtacgaaa aataaaatat accatttatt actggcaatt gattctgcca
     2821 cccccatatc aacatcaata aatgcaaccg agactgcacc aaatgtcagt actgttggaa
     2881 agtaggggat gacttgtagc caggtcataa gcattcctac aaaaccccag cgagccccaa
     2941 atgcttctcc tatccatcga aatgcccccc ctttttgtgg atatgttgta gctagctcag
     3001 ccgcaacaaa ggcaacaggt actaaaaaca taattgcagc aaaggtatag taaaaaatgg
     3061 cacctaagcc gtattctgct tcagaaggta taaatcgtag acttactaca gcagttatat
     3121 tcatgatagc gagtgtcccc actgtcattt tcttactttt gggtgcgata ttagcattag
     3181 acatttttat actctcgaac atttttaaaa tagaaacata taattacgat gtaaatataa
     3241 aaactaatat taactttaac acttatctta aaaacactaa cgcaatctgc tttacaaaaa
     3301 taagtaatac ctaaagtcgt aacgacacca atagtgtttt catcgacatg cttcaaaacc
     3361 tctttagcat tcattatcag gtgatgatca tcaagtggaa tttcacacaa tttcacatca
     3421 cagtaacggc agaatttatg ccagcatatt tgcacaggat cgcaaatcag gttcggttga
     3481 tttaccttgt acttcgcgtt ttttacacca ctgccatttt aacgccaagc ggccgagcat
     3541 agcagcttca gatgaaccaa tggttgagca acctaaggtg tgtcgcgaat cggggaaact
     3601 ccacgaatcg gaaagcatac gcacacagcg accttcaggt tccgccattt gtggatattc
     3661 atctttataa atcatgtttt tatcaatagc taaattaagc aactcacgga tttcatcatc
     3721 aagccatgtt tgacagaaag tagctaagtt ttgtcgtgaa tccccatcaa ggatgagctc
     3781 atcacgtatg gcgctgaata catttcgaga tttcttctca tctacacgca aatgatattt
     3841 atgcaatata aatgaaagct caagtgagct atgaat
//
LOCUS       DAZOZX010000232         3831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZOZX010000232 DAZOZX010000000
VERSION     DAZOZX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.237"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            52..1110
                     /gene="tsuA"
                     /locus_tag="ACHUPT_004007"
     CDS             52..1110
                     /gene="tsuA"
                     /locus_tag="ACHUPT_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0821339.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1124..1351
                     /gene="tsuB"
                     /locus_tag="ACHUPT_004008"
     CDS             1124..1351
                     /gene="tsuB"
                     /locus_tag="ACHUPT_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0821340.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1394..2818)
                     /gene="sbcB"
                     /locus_tag="ACHUPT_004009"
     CDS             complement(1394..2818)
                     /gene="sbcB"
                     /locus_tag="ACHUPT_004009"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0821341.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            3000..>3831
                     /locus_tag="ACHUPT_004010"
     CDS             3000..>3831
                     /locus_tag="ACHUPT_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0821342.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
BASE COUNT          915 a          901 c          997 g         1018 t
ORIGIN      
        1 ccaccagtca tgaaaaattt ccccattaac attcatctgg ttaattaatt tatgttttca
       61 atgatattaa gcgggctaat ttgtggtgct ctgctgggat ttgtcatgca gcgtgggcgt
      121 ttttgcctga ctggtggttt tcgcgatatg tatatcgcga aaaataatcg catgttttac
      181 gccttgctga ttgcaatttc ggcacaaagc gtgggggttt ttgcgttaat tcaggcgggc
      241 ctactgactt acgaagccgg ggcgttcccg tggcttggta ctgttatagg tgggtatctt
      301 ttcgggctgg gaattgttct ggcgggtgga tgtgccaccg ggacctggta tcgcgcgggt
      361 gagggattga tcggcagttg gatcgcgctt ttcacttata tggtgatgag tgcggtgatg
      421 cgttctccac acgccagtgg tttaaatcaa accttgcagc actacactac tgaacataac
      481 tctattgctg agacttttaa tttgtctgtg tggccgttgg ttaccgtttt gctggtgata
      541 acgctctggg tggtgatgaa agagttgaag aagccaaaac tcaaagtcgc gaccttaccg
      601 ccgcgccgaa ccgggatcgc tcatattctg tttgaaaaac gctggcatcc ctttgtcact
      661 gctgtactca tcggtttgat tgcgctttta gcctggcccc tgagtgaagc aaccggacgc
      721 atgtttgggt taggagtcac ttccccaacg gccaatattc ttcaatttct ggtcgcgggt
      781 gacgtgaaat acattaactg gggcgttttc ctggtgttag ggatcttcgt ggaggcattt
      841 attgcagcca aagcgagccg tgagttccgc gttcgcgcag ctgatgcaca aacaacatta
      901 cgtagcgggt taggtggtgt actgatgggc ttcggtgcca gtattgcagg tggttgctct
      961 atcggtaatg gactggttat gactgcaatg atgacctggc agggctggat tggccttgta
     1021 tttatgattc tcggagtctg gactgcgtcc tggcttgtgt atgttcgacc gcagcgtaag
     1081 gcgcgactgg caactgctgc ggcaaattaa gtaaggtgta aagatggcga ttaaaaagct
     1141 ggatgtggtg acgcaggtct gtccgtttcc actcatagaa gcaaaagcag cactggcaga
     1201 aatggccagc ggcgatgagt tggtaattga gtttgattgc acccaggcaa ctgaagcaat
     1261 cccacagtgg gcggcagaag aaggacatgc catcaccgat tatcagcaga ttggcgatgc
     1321 agcgtggagc atcaccgttc aaaaagcctg actcaacatt gtcctccgcc gtaccagcgg
     1381 cggcggcttc aaattagacg atctcttccg cgtactgcca aagtgctttt aacagcgcca
     1441 ctttctcttt gtcatcggca tattgttgta ccagcatctg caattcatca gcataaccct
     1501 gcaaaaactc tggcgtgaag acctggtggc ggtgctccag ccagcgttgc tgctcggcat
     1561 aatccagcgt ccccgggaag ttgcgtgccc gataattgaa caacaacttt tcaattcgtt
     1621 tatcaacaaa agtgatatcc agtgccggta aattacgcgg ctcggtttcc agcacaattt
     1681 tcattgctgc acgatcggcg tcactgaaaa agccattata aagctgtgca tccacattat
     1741 ctgaaggtgt aaacggttcg gcttccgcga atatcgctac cactttttca cgcacttgcg
     1801 gattttcacg cagaattttt aggttatcga ggcaatgctg acgattaatt cccagtcggt
     1861 cggcatcttc cgggcgtagc gtattcgcct gggccagtac cggacattta ttgatatgca
     1921 ccagcttaac cggaacggcg gcgttatcgc caagatcggc ttttgcggta tataaacgct
     1981 cgcgcaatgt gtcgatatcc agctccagta atggcgaaat gtctcctgcc aaatccgcca
     2041 taattacggc attgcgattt tcaggatgcc acgccagcgg tgccacccag ctggtattgc
     2101 cacgccatgc cccaaacatt ccggaaacgt gagccagggg tttcatctgc ggaacatcaa
     2161 tcaacgccat cagtttgtgt ttattacgat gggtaaagag ataatcaaac aggcgtggct
     2221 gacgcgtttt taccagtttc gccatcgcaa tagtggcgta cacatcagcc atcgcatcgt
     2281 gggcgttgct atgttcaata ccattcgctt tggttaaatg ctcaaggcga aagctcggta
     2341 gaccgtcatc attttcaggc cagtttattc cttccgggcg cagggcataa caggcacgca
     2401 taacatccag taaatcccag cgcgagttat catgctgcca gctccaggcg taaggatcgt
     2461 agaaattacg ataaaaaacg ttgcgtgtga cttcgtcgtc gaaacgcaca ttgttgtagc
     2521 ccagaataca ggtcttcggt acggtaaaaa gcgagtgaat acgggcggca aacgcggctt
     2581 cgttttctcc tttcgcccgt gcctcctgcg gggtaatacc ggtaattaat actgctccag
     2641 gccggggtaa atagtcatcc gcgggcttgc agtaaaagac ttcgggttcg ccgatgacat
     2701 tgaattcgtc atcggtgcga atggctgcga actgtgcagg gcgatctaac gcggggtgcg
     2761 tgccaaaggt ttcgtaatcg tgaaagagga aggtaggttg tttatctgtg tctgtcattg
     2821 tctagcattg tccactgtag ttaattaact gtctgttata actcgaaaag caaccctttt
     2881 ttcacctgag tatgtctacg aacgtcaaat atagttgttt actgtgcgct gttgtccatg
     2941 tgatattgtg taccagatgt gtgccaaact attatttctg agcgtaccaa atattattta
     3001 tggctataag cgacacaaaa ctgcgtacta tttatggtaa accatattcg ggcccacaag
     3061 aagtggctga tgccgatggc ctcagcgtac gaatttcacc gaagggggtc atccagttcc
     3121 agtaccgcta tcgctggcat ggcaagccta atcgacttgg gcttggtcga tacccatccc
     3181 tgtctttgaa ggatgccaga cagatcactg ctgacttgcg aaagctctat ttctcaggaa
     3241 cggatccacg cacctatttt gaagagaagg tggagaactc catgacggtc gcccagtgtc
     3301 tcgactactg gttcgacaac tacgtctcta caactctcag agaaaagacc caggcacttt
     3361 atcgatcaac ggttatgaag cgcatgcatg acgccttccc taatcgtccg gcatcttcta
     3421 tcacggttaa gcaatgggtt gacctgctta ccgaagaaga aaaagataat ccacgccgag
     3481 caaggcaggt gctaagtcaa ctaagatcag caattagttg gtgcatgcgg cgtcagttga
     3541 tagatagttg cgcaattatg agcatccagc caagggactt cggctcccgc gctgaggtag
     3601 gggatcgggt actgtcgtat cacgaactgg ctaagatttg gcttgctatt gaaagaagcc
     3661 gtgcgtctac gtcaaataag ctccttcatc agatgcttat gctgtggggg gcgaggctct
     3721 cagagcttag gctggcaaca aagacagaat ttgacctgct ggacaacgta tggaccgtac
     3781 cgaaagagca tagcaagatg ggtaatgtta tccgccgtcc aatcttcgaa c
//
LOCUS       DAZOZX010000233         3807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZOZX010000233 DAZOZX010000000
VERSION     DAZOZX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.238"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            437..2090
                     /gene="ushA"
                     /locus_tag="ACHUPT_004011"
                     /pseudo
     CDS             437..2090
                     /gene="ushA"
                     /locus_tag="ACHUPT_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
     gene            complement(2101..2271)
                     /locus_tag="ACHUPT_004012"
                     /pseudo
     CDS             complement(2101..2271)
                     /locus_tag="ACHUPT_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2404..2523)
                     /locus_tag="ACHUPT_004013"
                     /pseudo
     CDS             complement(2404..2523)
                     /locus_tag="ACHUPT_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2597..3596)
                     /locus_tag="ACHUPT_004014"
                     /pseudo
     CDS             complement(2597..3596)
                     /locus_tag="ACHUPT_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1053 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tcataattgg ttatgatgca atggccgtcg
      781 gtaatcatga atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat
      841 ttcctttcct ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat
      901 ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata
      961 ctgcgaaaac aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg
     1021 aggcgaggag tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg
     1081 caacacatat gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg
     1141 aaatggctcg cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag
     1201 ctcctgtctg catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct
     1261 gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg
     1321 tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac
     1381 acccagttaa tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt
     1441 acacccccga aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata
     1501 aaggcaaagc acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca
     1561 gaagtaaagt tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag
     1621 aacgtattga cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga
     1681 gtggtaacat cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa
     1741 gtattgattt gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac
     1801 cggattccgg agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa
     1861 tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa
     1921 catttagctt taatgctact ggtggggatg gctatccacg tattgataat agacccggat
     1981 acataaatac cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac
     2041 cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt
     2101 gacgatcatc acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg
     2161 tatgtttgcg atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt
     2221 gctgaattta accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt
     2281 cgctgattaa agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat
     2341 tgatatttac taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc
     2401 cggtcagtcc tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt
     2461 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga
     2521 accccgccgg agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc
     2581 gtcatacggc tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct
     2641 tcggatactg tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt
     2701 atttagactg catccactta agcaaacgtg actgcattgc actccacagt cgatagctga
     2761 agtttctgga ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc
     2821 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
     2881 tttctgttat tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt
     2941 cgcctttgaa attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca
     3001 tcatcggcgt acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag
     3061 gcatagtgca ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct
     3121 cgaccccgtg ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca
     3181 atgtacatca ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca
     3241 tgatcgatat catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag
     3301 cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac
     3361 gagtctgcat ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg
     3421 gcgatccggt ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga
     3481 atgtccacac gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt
     3541 ttgtagagat tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct
     3601 gccgccccac ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca
     3661 ggaaatggct ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc
     3721 gccgcaccat cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc
     3781 gctactacgg tttactccgt ccctgta
//
LOCUS       DAZOZX010000234         3799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZOZX010000234 DAZOZX010000000
VERSION     DAZOZX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.239"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            32..295
                     /locus_tag="ACHUPT_004015"
                     /pseudo
     CDS             32..295
                     /locus_tag="ACHUPT_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            341..550
                     /gene="hha"
                     /locus_tag="ACHUPT_004016"
     CDS             341..550
                     /gene="hha"
                     /locus_tag="ACHUPT_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR0821343.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            589..1179
                     /locus_tag="ACHUPT_004017"
     CDS             589..1179
                     /locus_tag="ACHUPT_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0821344.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1419..1679
                     /locus_tag="ACHUPT_004018"
     CDS             1419..1679
                     /locus_tag="ACHUPT_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0821345.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1903..1977
                     /gene="tap"
                     /locus_tag="ACHUPT_004019"
     CDS             1903..1977
                     /gene="tap"
                     /locus_tag="ACHUPT_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0821346.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1970..2827
                     /gene="repA"
                     /locus_tag="ACHUPT_004020"
     CDS             1970..2827
                     /gene="repA"
                     /locus_tag="ACHUPT_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0821347.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3528..3719
                     /locus_tag="ACHUPT_004021"
     CDS             3528..3719
                     /locus_tag="ACHUPT_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821348.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1036 a          925 c          947 g          891 t
ORIGIN      
        1 gctcattatt agctccttct gatgccattc tatttcagga aggagtgtcc gttaaactca
       61 ggctacctca tattttcagc gagatcgtta tggtcgtatt ctcgggcagg tttatgcacc
      121 agacggaatg aatattaatc agtttatggt ccgggccggg gctgcctggg tatatgagca
      181 gtacaacaca gatcccgtgc tacctgtcct tcagaacgaa gccagacaac aaaaacgtgg
      241 gctctggtca gatgctgatc cggttccccc ctggatatgg aggcaccgta aataaagcga
      301 tggcaatcct atcattcatc tgttctcagc gggaataaaa atggcaaaaa caaaacagga
      361 atggctgtat cagttacggc gatgttcatc agtcaatacg ctggaaaaaa ttattcataa
      421 aaacagagac agtttatcaa acagtgaacg tgaatcattt aattcagcag cagaccaccg
      481 gctagcggaa ttaatcaccg gaaagttata tgacaggata ccaaaagaga tatggaaata
      541 tgtaaggtaa taattcagca ataattaatt gccaggagca gataaataat gaagttgatt
      601 atttttattc tcattgttct gattattgca gcgctactta tccggattat cctcagaagt
      661 gtaaatcagc attctccttt gctgatgcaa ttgcatgcgg caggtatacg gactggtgat
      721 gcagagcgaa tactgtccag cggtgaatac tggcaacgtc agaagacgct gctgacagaa
      781 agggaagtca gttttatgaa aggactgttc agaattgtgg atatgaagcg gtggtatctg
      841 tgtccgcagg tacgggtagc ggatatcgtc cagctgaacg ggaatatccg gccacgatca
      901 cgccagtggt ggcagttatt caggatggtg tctcagtggc atgttgatgt ggttatcgtt
      961 gagctgcgtt cgttcagtat tgttgcagca gtagagctgg atgatgccag ccatttacga
     1021 ccggaacgca gacgccggga tattcttctg gaagaggttc tgaggcaggc tggtattccg
     1081 ttgctcagaa gccacgatgc cagaaaactg ctgcagatga ccggagaatg gctgaataca
     1141 acaggggctg atcagcagtc accggaacat cgtagctgac gccttcgcgt tgctcagttg
     1201 tccaaccccg gaaacgggaa aaagcaagtt ttcctcgctc ccggcgtttc cataactgaa
     1261 aaccatacta tttcacagtt taaatcacat taaacgacag taatccccgt tgatttgtgc
     1321 gccaacacag atcttcgcca caattctcaa gtcgctgatt tcaaaaaact gtagtatcct
     1381 ctgcgaaacg atccctgttt gagtattgag gaggcgagat gtcgcagaca gaaaatgcag
     1441 tgacttcctc attgagtcaa aagcggtttg tgcgcagagg taagcctatg actgactctg
     1501 agaaacaaat ggccgctgtt gcaagaaaac gtcttacaca caaagagata aaagtttttg
     1561 tcaaaaatcc tctgaaagat ctcatggttg agtactgcga gagagagggg ataacacagg
     1621 ctcagttcgt tgagaaaatc atcaaagatg aactgcagag actggatata ctaaagtaaa
     1681 gactttactt tgtggcgtag catgctagat tactgatcgt ttaaggaatt ttatggctgg
     1741 ccacgccata aggtggcagg gaactggttc tgatgtggat gtacaggagc cagaaaagca
     1801 aaaaccccga taatcttctt taactttggc gagtcagaaa gattaccggg gcccacttaa
     1861 accgtatagc caacaatcaa gctatgcggg gagtatagtt atatgcccgg aaaagttcaa
     1921 gacttctttc tgtgctcgct ccttctgcgc attgtaagtg caggatggtg tgactgatct
     1981 tcaacaaact tattaccgcc aggtaaagaa cccgaatccg gtgttcacac cccgtgaagg
     2041 tgcccggacg cttccgttct gcgggaaact gatggaaaag gcggtgggct tcacctcccg
     2101 ttttgatttc gccattcatg tggcacatgc ccgttcgctt ggtctgcgtc ggcgcatgcc
     2161 accggtacta cgtcgccggg ccattgatgc gctgctgcag gggctgtgtt ttcactatga
     2221 cccactggcc aaccgcgtcc agtgttccat caccacgctg gccattgagt gcggactggc
     2281 gacggagtct gctgccggaa aactctccat cacccgggcc acccgggccc tgacgttcct
     2341 tgcagagctg ggactgatta cctaccagac ggaatatgac ccgcttatcg ggtgctacat
     2401 tccgaccgat atcacgttca catctgcact gtttgctgcc ctcgatgtat cagaggaggc
     2461 agtggccgcc gcgcgccgca gtcgtgtgga atgggaaaac agacagcgca aaaagcaggg
     2521 gctggatacc ctgggtatgg atgaactgat ggcgaaagcc tggcgttttg ttcgtgagcg
     2581 tttccgcagt taccagacag agcttaagtc ccgtggaatg aagcgtgccc gtgcgcgtcg
     2641 tgatgcggac aggcagcgtc aggatattgt caccctggtg aaacggcaac tgacgcgtga
     2701 aatctcggaa gggcgcttca ctgccagtcg tgaggcggta aaacgtgaag tggagcgtcg
     2761 tgtgaaggag cgcatgattc tgtcacgtaa ccgtaattac agccggctgg ccacagcttc
     2821 cccctgaaag tgacctcctc agaataatcc ggcacgcgcc ggaggcattc gcacgcctga
     2881 agcccgccag tgcacaaaaa atcagcgcta catacaaaaa acaacctcat catccacctt
     2941 caggtgcatc cggtcccccc tgtttttgat acaaaacacg cctcacagac ggggaatttt
     3001 gcttatccac attaaactgc aagggacttc accataaggt tacaaccgtt cacgtcataa
     3061 agcgccagac gtcaacccta cagggtgcaa tgtatctttt aaacacctgt ttatatctcc
     3121 tttaaactac ttaattacat tcatttaaaa agaaaacctg ttcactgcct gtcctgtgga
     3181 cagacaaata tgcacctccc accgcaagcg gcgggccccc aaccgaagcc ggtttagtta
     3241 caacatgcaa acataaaccc ctaaaaaacc caagtccaac gcaaaactga aaccacaacg
     3301 cccccctcat aactgaaaag cgccgccgcc cccgcccaaa gggcgggaac agagtcgctt
     3361 ttaattatga atgttgtaac tacatcatca tcgctgtcag tcttctggct ggacgtatcg
     3421 aatacacgct cgtaagcggc cctcacggcc cgctaacgcg gagatacgcc ccgacttcgg
     3481 gtaaaaccct cgtcgggacc actccgaccg cgcacagaag ctctatcatg gctgaaaacg
     3541 ggtatggctt ggcagggctg gggatgggta aggtgaaatc tgtcaatcag taccggctga
     3601 cgccgggctt cggcggtttt actccggtat cacatgtaac cacagcgtgc cgccttccat
     3661 gccgctggcg cggcatcaga ataatacaag cagcatttaa tgcatttgca aaagtgtgag
     3721 taatattaag tggtcgtttc agggatatcg ttaagacttg agcgatagga attaaaaccc
     3781 caaaaagatt aaaaaaaca
//
LOCUS       DAZOZX010000235         3787 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZOZX010000235 DAZOZX010000000
VERSION     DAZOZX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.240"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            2..256
                     /gene="yoeB"
                     /locus_tag="ACHUPT_004022"
     CDS             2..256
                     /gene="yoeB"
                     /locus_tag="ACHUPT_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0821349.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            339..1163
                     /locus_tag="ACHUPT_004023"
     CDS             339..1163
                     /locus_tag="ACHUPT_004023"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0821350.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1209..2192
                     /locus_tag="ACHUPT_004024"
     CDS             1209..2192
                     /locus_tag="ACHUPT_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0821351.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            2353..2415
                     /gene="yoeI"
                     /locus_tag="ACHUPT_004025"
     CDS             2353..2415
                     /gene="yoeI"
                     /locus_tag="ACHUPT_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0821352.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            2405..3763
                     /gene="plaP"
                     /locus_tag="ACHUPT_004026"
     CDS             2405..3763
                     /gene="plaP"
                     /locus_tag="ACHUPT_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0821353.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT          890 a          896 c          999 g         1002 t
ORIGIN      
        1 agtgaaacta atctggtctg aggaatcatg ggatgattat ctgtactggc aggaaacaga
       61 taagcgaatt gttaaaaaga tcaatgaact tatcaaagat acccgcagaa caccatttga
      121 aggtaagggg aaaccagaac ccctgaaaca taatttgtca ggtttctggt cccgacgcat
      181 tacagaggag caccgtctgg tatacgcggt tactgatgat tcactgctca ttgcagcatg
      241 tcgttatcat tattgaacag attcattcat agaatgaatt gtcagttttg atacgctagc
      301 gtagccttat agatctaagg tgtatcagga gataacggat gaaaaaggtc gcaattgtcg
      361 ggttagggtg gttaggtatg ccgctggcga tgtcactttc agcgcgaggc tggcaagtca
      421 ccgggagtaa aaccacacaa gatggtgtcg aagcggcccg aatgagtggc attgatagct
      481 atctgcttcg catggagcct gagttagttt gcgattctga cgatctggat gcccttatgg
      541 atgccgatgc gctggtcatt acgcttccgg cacgtcgtag cggccccggc gatgagttct
      601 atttacaagc ggtacaagag ttagtggata gcgcgctggc ccatcgtatt ccccgaatta
      661 tttttaccag ctcaacatct gtctatggcg acgcgcaagg cacggttaaa gaaactaccc
      721 cgcgtaatcc ggtaaccaac agtggacgag tattagaaga actcgaagac tggctgcaca
      781 atttacccgg tacttcggtc gatattctgc gtcttgcggg cctggtcgga ccgggacgtc
      841 atccgggacg cttctttgcc ggaaaaaccg cccctgatgg tgaacatggt gttaatttag
      901 tccatttaga agatgttatt ggcgctatca ctctgttgtt acaggcacct aaaggcggac
      961 acatctataa tatatgtgcg ccagctcacc ctgcgcgtaa tgttttctat ccgcagatgg
     1021 cccgtttact ggggctgcaa cctccgcagt tcagaaatag tctggacagc ggcaaaggta
     1081 agattattga tggcagtcgg atttgtaatg aactgggatt tgaataccag tatcccgatc
     1141 cgctggtaat gccgttggag taaacgatca cgccagcagg acgccgcatg cactgcaagg
     1201 gggcgtgtat gaaaccactg ctggatgtgt tgatgatcct cgatgccata gaaaaagagg
     1261 gcagttttgc ggcggcgtca gccaaactct acaagacgcc gtccgctctg agttacaccg
     1321 ttcacaagct ggaaagcgac ctaaatattc aattgcttga tcgtagcgga caccgcgcta
     1381 aattcacccg caccggaaaa atgttattag agaaagggcg ggaagttctg cataccgtgc
     1441 gagaactgga aaagcaggcg atcaaactgc atgaaggctg ggaaaacgag ctggtgattg
     1501 gcgtggacga cacgtttcct ttttctctgc ttgcgcctct cattgaagct ttttatcaac
     1561 atcatagcgt gacgcgcctg aagtttatca atggcgtact cggtggttcc tgggatgccc
     1621 tgactcaggg gcgagcggat attatcgttg gcgcgatgca tgagccacca tcttccagtg
     1681 agtttggttt ctcgcggttg ggcgatcttg aacaagtctt tgcggtcgcg cctcatcatc
     1741 cgttagccca ggaagaagaa ccgttaaacc gtcgtatcat taaacgatat cgggcgattg
     1801 tggtggggga tactgcgcag gcaggcgctt ctacggcctc acaacttctc gacgaacaag
     1861 aagccattac cgttttcgat tttaaaacca aactggagct gcaaattagc ggccttggct
     1921 gcggctattt accacggtat ctggcgcaac gttttctcga tagtggcgcg ttaatcgaga
     1981 agaaagtggt cgcccaaact ctctttgaac ctgtctggat tggctggaat gaacagaccg
     2041 cagggcttgc cagcggctgg tggcgagatg aaattttagc aaatagtgcg atcgccggtg
     2101 tttatgcaaa atctgatgac ggaaaatcag ccattcaaag aaaaattatt atgacaagcc
     2161 tctcattctc ttgtcatttc ccccccattt aggcacaatg cgccgctgtc aaaaaatgat
     2221 taaaaaccga cgtttaatta gcgtcggtta ttttttgctt caaaccaatc attcatacca
     2281 agaggccggg cttcgtaccg gatagatatt tactaaaaat cgacagttgt tgtcgctgag
     2341 gaatccaaaa aaatggggca attttttgct tacgcgacgg ttatcaccgt aaaggagaat
     2401 aaccatgtcg cataacgtta ctccaaacac ctctcgcgtg gaattgcgta aaacgcttac
     2461 gttagttccg gttgtaatga tgggtctagc ctatatgcag ccgatgacgc tgtttgatac
     2521 atttggtatc gtttcaggcc tcacggatgg tcatgtgccg acagcctatg cgttcgcatt
     2581 gattgcgatc ctgtttacgg ctctgagcta cgggaagctg gttcgccgct atccttctgc
     2641 tggctctgca tacacttacg cccagaaatc cattagcccg actgtcggct ttatggtggg
     2701 ttggtcttct ctgctcgact atctgttcgc gccgatgatc aacattctgc tggcgaaaat
     2761 ctattttgaa gctctggtgc cttccattcc atcgtggatg tttgtggtgg cgctggtggc
     2821 ctttatgacc gcctttaacc tgcgtagtct gaaatccgta gcgaacgtca acactgtaat
     2881 cgtcgtgttg caggtagtgc tgatcgcggt gattctgggc atggttgttt atggcgtatt
     2941 tgaaggtgaa ggcgctggta cgctggcgag cacgcgtccg ttctggtctg gcgatgcgca
     3001 tgttatcccg atgattaccg gggcgacaat cctgtgcttc tcctttaccg gctttgacgg
     3061 catcagcaat ctgtcggaag aaaccaaaga tgcagagcgc gtgatcccgc gtgcgatttt
     3121 cctgaccgcg ctgattggcg gcatgatctt catctttgca acttacttcc tgcagctgta
     3181 cttcccggat atctctcgct ttaaagatcc ggatgcgtca cagcctgaaa tcatgctgta
     3241 cgttgcgggt aaagcgttcc aggttggcgc gctgatcttc tccaccatta ccgtactggc
     3301 gtccggtatg gcggcgcacg caggcgtagc gcgtctgatg tacgtaatgg gtcgtgacgg
     3361 cgtgttcccg aaaagcttct tcggttatgt acacccgaaa tggcgtactc cggcgatgaa
     3421 catcatcctg gttggcgcga ttgccctgct ggcaatcaac ttcgacctgg taatggcaac
     3481 ggcgctgatt aactttggtg cgctggtggc gttcaccttt gttaacctgt cggtcatctc
     3541 gcagttctgg atccgtgaga agcgtaacaa gacgctgaaa gatcacttcc agtatctgtt
     3601 cctgccgatg tgtggtgcgc tgaccgttgg tgcgctgtgg gttaacctgg aagaaagctc
     3661 aatggttctg ggtctgatct gggctgctat cggtctgatt tacctggctt gcgtcaccaa
     3721 gagcttccgt aatccggttc cgcagtacga agacgtagca taatcatctt ttctaataat
     3781 aaaccgg
//
LOCUS       DAZOZX010000236         3691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZOZX010000236 DAZOZX010000000
VERSION     DAZOZX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.241"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            108..2105
                     /locus_tag="ACHUPT_004027"
     CDS             108..2105
                     /locus_tag="ACHUPT_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR0821354.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="ACHUPT_004028"
     CDS             2168..3445
                     /locus_tag="ACHUPT_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR0821355.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          891 a          839 c          931 g         1030 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg t
//
LOCUS       DAZOZX010000237         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZOZX010000237 DAZOZX010000000
VERSION     DAZOZX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.242"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..475
                     /locus_tag="ACHUPT_004029"
     CDS             <1..475
                     /locus_tag="ACHUPT_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR0821356.1"
                     /translation="VLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMMAEWLGGDVS
                     PGINVGYIFADADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAPAKLITTARY
                     GKERLVKLSAMDRAKAVKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEPCDNGGE"
     gene            476..1894
                     /locus_tag="ACHUPT_004030"
     CDS             476..1894
                     /locus_tag="ACHUPT_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR0821357.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            1894..2742
                     /locus_tag="ACHUPT_004031"
     CDS             1894..2742
                     /locus_tag="ACHUPT_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR0821358.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            2742..3197
                     /locus_tag="ACHUPT_004032"
     CDS             2742..3197
                     /locus_tag="ACHUPT_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR0821359.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            3200..>3627
                     /locus_tag="ACHUPT_004033"
     CDS             3200..>3627
                     /locus_tag="ACHUPT_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR0821360.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
BASE COUNT          940 a          895 c          943 g          849 t
ORIGIN      
        1 agttcttgcg gcattacgta agctcggtgt ggcatcaaat gccacgttaa ccgatgtaga
       61 accgcagtct atggaagacg gcgtcaacga ccttgaaatg atgatggctg aatggcttgg
      121 cggtgatgtg tcacctggta tcaacgttgg ctacattttc gctgatgcag atgtcgctcc
      181 agaaccgggc gatgagcacg gtttatcaaa taacgctatc aatgccgtca ttttcaacct
      241 tgcctgccgc attgctccag attatgcgct ggaagcgcca gcaaaactta taaccactgc
      301 cagatacggg aaagagcgac tcgtcaaact gtctgcaatg gacagagcaa aagccgttaa
      361 atgtaagtcc ggttatccaa accgtatgcc tgttggtagc ggtaaccagt tggcgaagtg
      421 gaatggttgg aattacttcc accggaagga accttgcgat aacgggggcg aataaatgcc
      481 gattcagcaa cttccgctta tgaaaggtgt cggcaaagat ttccgaaacg ccgactacat
      541 cgactatctg ccagtgaata tgctggctac acccaaagaa atactcaaca gcagcggata
      601 tcttcgctca ttcccgggca ttgccaaacg ttctgatgtg aatggtgtat cgcgcggtgt
      661 cgagtacaac atggcgcaga atgctgttta tcgcgtttgc ggtggcaagt tgtataaggg
      721 agaaagcgag gtcggtgatg ttgccggaag tggtcgcgta tcaatggcgc atggtcggac
      781 atcacaggcg gtaggcgtta atggtcaact ggtcgagtat cgctatgatg gcacggttaa
      841 aaccgtctca aactggccta cagacagtgg attcactcag tatgagttag gttcagttcg
      901 cgacattacg cgcttacgtg ggcgttatgc gtggtcaaaa gacggcactg attcatggtt
      961 tatcactgac cttgaagatg aatcgcatcc tgaccgctac agtgcacaat atcgtgccga
     1021 gtctcagccg gacggcatca tcggcatagg tacatggcga gacttcatcg tctgctttgg
     1081 ctcatcgacg attgaatatt tttccctgac aggtgcaacc accgttggtg ccgctttgta
     1141 tgtcgcacag ccatcgctga tggtgcagaa aggtattgcc gggacttact gcaaaacacc
     1201 attcgctgat tcttatgcgt tcatcagcaa tccggcaaca ggtgcgccgt ctgtatacat
     1261 catcggctcc ggtcaggtgt caccaatcgc cagcgcgagc attgagaaaa tcctccgctc
     1321 ctacactgct gatgaactgg ctgatggcgt gatggaatcg ctgcggtttg atgcgcatga
     1381 gttgctgatt atccaccttc cgcgccatgt actggtgtac gacgcatctt caagcgccaa
     1441 tggtccgcaa tggtgtgtgc tgaaaacagg cctgtatgac gatgtgtacc gcgctatcga
     1501 cttcatttac gaaggcaatc aaataacgtg cggcgataag ctggagtccg tgaccgggaa
     1561 attgcaattc gacatcagca gccagtacga caagcaacag gaacacctgc tgtttactcc
     1621 actgttcaaa gcggataacg ccagagtgtt cgaccttgag gttgaatcgt caactggcgt
     1681 tgcgcagtat gctgaccgcc tttttctctc tgcaaccact gacggcatca attacgggtg
     1741 tgagcagatg attgagcaga atgaaccgtt cgtttacgac aaacgcgttt tgtggaagcg
     1801 agtagggcgc atcaggaaaa atgtcggctt caaattgcgc gttatcacga agtcacctgt
     1861 cactctgtct ggctgccaga taaggattga gtaatggcgg attcgaatct caatgtgccg
     1921 gtaatcattc aggctacacg gctcgacaca tcagtccttc cacgcaatat cttctcgcag
     1981 tcgtatctgc tttacgttat cgcacagggc actgatgttg gtaacgtggc caacaaggcc
     2041 aacgaggccg gacagggcgc ttatgacgca caagtcagga acgatgagca ggatgtgatt
     2101 ctcgctgacc atgagcagcg aatttctgct gcggaagcaa cgcttgttaa tcatgaggag
     2161 cgaatcagcc aggcagaatc aactcttcag gaacatgaaa cgcgaatcgc tcagaatgaa
     2221 agcgatattg cgtcgcttga taccagagtt cagtcgctgg aatcgcaggt ttcagaccat
     2281 gaaacgcgca tcgatgctct ggagtatgcc actactcgca agaagtcaga ggttgtttac
     2341 tctggcgtat ctgtaaccat cccgacagcg ccgaccaacc ttgttagcct actgaaaacg
     2401 ctcacgccgt catccggcac gttggcacca ttcttcgaca ccgttaacaa caagatggtt
     2461 gtgttcaacg agaacaaaac cttgttcttc aagctgtcga tcgtcgggac gtggcccagc
     2521 ggaaccgcca acaggtcaat gcagctaacc ttttccggct ctgttcctga tacgctggtc
     2581 agcagtcgta atgcggcgac aacaaccgac aacatcctgt tagctacatt cttcagcgtg
     2641 gataaagacg gatttcttgc cacaaatggc agtacgttaa ccattcagtc gaatggtgcg
     2701 gcgtttactg ccacaaccat caagataatc gcggagcagt gatgattcag ttcaaaccaa
     2761 cgcgaaacat cgacctgata gaagccgtgg gaaatcaccc cgacattatc gccgggagca
     2821 acaacggtga tggatacgac tacaagcctg aatgccgtta cttcgaggtg aacgtgcacg
     2881 ggcagttcgg cggcattgtt tactatcagg agattcagcc tttgaccttt gattgccacg
     2941 ccatgtacct gccagaggtt cgtggattca gcaaggaaat cgggctggcg ttctggcgat
     3001 acattctgac taacaccacc gttcagtgcg tcacatcgtt cgctgcgcgc aaattccgcc
     3061 acggtcagat gtactgcgca atgattggcc ttaagcgagt aggaaccatc aagaaatact
     3121 tcaaaggcgt ggatgacgtg acattttaca gtgctacacg cgaagaacta atcgacttcc
     3181 tgaatcacgg gagatagcca tgttatatgc atttaagctg ggcagaaaac tgcgcggcga
     3241 ggaaccttat tgccctgaaa aaggcgggaa aggtggcagc tctgataaaa gtgcaaagta
     3301 tgcagcagaa gctcagaagt atgccgcaga cctgcaaaat cagcagtggc agacgatcat
     3361 gaaaaacctt gctccgttca cgccgcttgc ggagcagtat gttaaccagc ttcagaacct
     3421 ttccagttta gaaggacagg gtcaggcact taatcagtat tacaactctc agcagtataa
     3481 agaccttgca ggtcaggctc gttaccagag tcttgctgct gcggaggcta cgggaggact
     3541 tggttcgaca gccacaagca atcaactggc tacgattgct ccgactctcg gtcagtcgtg
     3601 gttatcaaac cagatgagca attacaa
//
LOCUS       DAZOZX010000238         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZOZX010000238 DAZOZX010000000
VERSION     DAZOZX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.243"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..628)
                     /locus_tag="ACHUPT_004034"
     CDS             complement(<1..628)
                     /locus_tag="ACHUPT_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0821361.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCV"
     gene            874..1635
                     /locus_tag="ACHUPT_004035"
                     /pseudo
     CDS             874..1635
                     /locus_tag="ACHUPT_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1742..2254)
                     /locus_tag="ACHUPT_004036"
     CDS             complement(1742..2254)
                     /locus_tag="ACHUPT_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821362.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(2393..3541)
                     /locus_tag="ACHUPT_004037"
     CDS             complement(2393..3541)
                     /locus_tag="ACHUPT_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0821363.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         1103 a          797 c          641 g         1086 t
ORIGIN      
        1 gcacacaggg taaatgcttt ttcgaaattt cagcggcctc tatttgagat ttagcgatgc
       61 caatctcgtt aagattctct gaaagatata aatcacaggt gtcaatctta cctgatttaa
      121 ccaatggata atccgttata ctgtaggtat ctttaaaagc cacggcaaac atttcaataa
      181 taacaattac taccacaagc gaaatcaaaa tgcgaaaaag aggaatactc cttttgcgct
      241 ctactttttc aacaacatct tcttgtaaat gagaatcatc ataagattgt ttacttattg
      301 ttatgttttc actgatgtaa aagcctactt tgggtatcgt aagaatcaca ttttcttcaa
      361 caccaatatt tttaaaactt ttgcgtaaca gggatatata ttggttcaat gtgttgtttg
      421 acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg tcaaccgctc
      481 caccattact ttccagtaac actgccaaaa tacaccccgc cgtagctgga aaatttatct
      541 catcctgggt atttacattc gagagcgagc atttgttcgg atcaaaaata atcgcgtcat
      601 tgataatgaa aaaacatctc cgctccatgc aggcaacaac acaaaataag attttgtcat
      661 tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata accctgaaaa
      721 agtcatataa aacttatatc cacagaataa aaaatcaaac atgtcagttt tatcaatata
      781 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
      841 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
      901 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
      961 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
     1021 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
     1081 tcataaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
     1141 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
     1201 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
     1261 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
     1321 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
     1381 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
     1441 gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
     1501 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
     1561 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
     1621 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
     1681 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
     1741 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
     1801 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
     1861 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
     1921 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
     1981 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
     2041 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
     2101 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
     2161 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
     2221 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
     2281 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
     2341 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
     2401 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
     2461 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
     2521 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
     2581 tgctataact aatcacagcg ccgaagggga tgggtttacc ctctttctgt tttaaggtta
     2641 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
     2701 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
     2761 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
     2821 tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
     2881 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
     2941 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
     3001 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
     3061 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
     3121 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
     3181 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttccgat
     3241 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
     3301 tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg
     3361 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
     3421 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
     3481 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagaca
     3541 atagactggc cccctgaatc tccagacaac cagtatcact tatttaagtg atagtcttaa
     3601 tactagtttt tagactagtc attggag
//
LOCUS       DAZOZX010000239         3548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZOZX010000239 DAZOZX010000000
VERSION     DAZOZX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.244"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUPT_004038"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUPT_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0821364.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHUPT_004039"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHUPT_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0821365.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUPT_004040"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUPT_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0821366.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIKNYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          819 g          951 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tttataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAZOZX010000240         3542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZOZX010000240 DAZOZX010000000
VERSION     DAZOZX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.245"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            12..1163
                     /gene="yiaY"
                     /locus_tag="ACHUPT_004041"
     CDS             12..1163
                     /gene="yiaY"
                     /locus_tag="ACHUPT_004041"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR0821367.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1328..2866
                     /gene="aldB"
                     /locus_tag="ACHUPT_004042"
     CDS             1328..2866
                     /gene="aldB"
                     /locus_tag="ACHUPT_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR0821368.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3085..3228)
                     /locus_tag="ACHUPT_004043"
     CDS             complement(3085..3228)
                     /locus_tag="ACHUPT_004043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821369.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3411..>3542
                     /locus_tag="ACHUPT_004044"
     CDS             3411..>3542
                     /locus_tag="ACHUPT_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0821370.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          882 a          845 c          936 g          879 t
ORIGIN      
        1 aggagtgtgt tatggcatct tcaactttct ttattccttc tgtgaatgtc atcggcgctg
       61 attcattgac tgatgcaatg aatatgatgg cagattatgg atttacccgt accttaattg
      121 tcactgacaa tatgttaacg aaattaggta tggcgggtga tgtgcaaaaa gcactggaag
      181 aacgcaatat ttttagcgtt atttatgatg gcacccaacc taaccccacc acggaaaacg
      241 tcgccgcagg tttgaaatta cttaaagaaa ataattgcga tagcgtgatc tccttaggcg
      301 gtggttctcc acacgactgc gcaaaaggta ttgcgctggt ggcagccaat ggcggcgata
      361 ttcgcgatta cgaaggcgtt gaccgctctg caaaaccgca gctgccgatg atcgccatca
      421 ataccacggc gggtacggcc tctgaaatga cccgtttctg catcatcact gacgaagcgc
      481 gtcataacaa aatggcgatt gttgataagc acgtgactcc gctgctttct gtcaatgact
      541 cctctctgat gattggtatg ccgaagtcac tgaccgccgc aacgggtatg gatgccttaa
      601 cgcacgctat cgaagcgtat gtttctattg ccgccacgcc gatcactgac gcttgtgcac
      661 tgaaagccgt gaccatgatt gccgaaaacc tgccgttagc cgttgaagat ggcagtaatg
      721 cgaaagcgcg tgaagcaatg gcttatgccc agttccttgc cggtatggcg ttcaataatg
      781 cttctctggg ttatgttcat gcgatggcgc accagctggg cggtttctac aacctgccac
      841 acggtgtatg taacgccgtt ctgctgccgc atgttcaggt attcaacagc aaagtcgccg
      901 ctgcacgtct gcgtgactgt gccgctgcaa tgggcgtgaa cgtgacaggt aaaaacgacg
      961 cggaaggtgc tgaagcctgc attaacgcca tccgtgaact ggcgaagaaa gtggatatcc
     1021 cggcaggcct acgcgacctg aacgtgaaag aagaagattt cgcggttctg gcgactaatg
     1081 ccctgaaaga tgcctgtggc tttactaacc cgatccaggc aactcacgaa gaaattgtgg
     1141 tgatttatcg cgccgcaatg taatcatcat tccaacaacg gctggcaaat tgtcagccgc
     1201 tttttcaact atctctgtaa tccttgcccg taaattcgtg atagctgtcg taaagctgtt
     1261 accgactggc gaagatttcg ccagtcacgt ctacccttgt tatacctcac accgcaagga
     1321 gacgatcatg accaataatc ccccttcagc acagattaag cccggcgagt atggtttccc
     1381 catcaagtta aaagcccgct acgacaactt tattggcggt gaatgggtag cccctgccga
     1441 tggtgagtat taccagaatc tgacgccggt gaccgggcag ctgctgtgcg aagtggcgtc
     1501 ttcgggcaaa cgagacatcg atctggcgct ggatgctgcg cacaaagtga aagataaatg
     1561 ggcgcacacc tcggtgcagg atcgcgcggc gattctgttt aagattgccg atcgaatgga
     1621 acaaaacctc gagctgttag cgacagctga aacctgggat aacggcaaac ccattcgcga
     1681 aaccagtgct gcggatgtac cgctggcgat tgaccatttc cgctatttcg cctcgtgtat
     1741 tcgggcgcag gaaggtggga tcagtgaagt tgatagcgaa accgtggcct atcatttcca
     1801 tgaaccgtta ggcgtggtgg ggcagattat cccgtggaac ttcccgctgc tgatggcgag
     1861 ctggaaaatg gctcccgcgc tggcggcggg caactgtgtg gtgctgaaac ccgcacgtct
     1921 taccccgctt tctgtactgc tgctaatgga aattgtcggt gatttactgc cgccgggcgt
     1981 ggtgaacgtg gtcaatggcg caggtgggga aattggcgaa tatctggcga cctcgaaacg
     2041 catcgccaaa gtggcgttta ccggctcaac ggaagtgggc caacaaatta tgcaatacgc
     2101 aacgcaaaac attattccgg tgacgctgga gttgggcggt aagtcgccaa atatcttctt
     2161 tgctgatgtg atggatgaag aagatgcctt tttcgataaa gcgctggaag gctttgcact
     2221 gtttgccttt aaccagggcg aagtttgcac ctgtccgagt cgtgccttag tgcaggagtc
     2281 aatctacgaa cgctttatgg aacgcgccat ccgccgtgtc gaaagcattc gcagcggtaa
     2341 cccgctcgac agcgtgacgc aaatgggcgc gcaggtttct cacgggcaac tggaaaccat
     2401 cctcaactac attgatatcg gtaaaaaaga gggcgctgac gtgctcacag gcgggcggcg
     2461 caagctgctg gaaggtgaac tgaaagacgg ctactacctc gaaccgacga ttctgtttgg
     2521 tcagaacaat atgcgggtgt tccaggagga gatttttggc ccggtgctgg cggtgaccac
     2581 cttcaaaacg atggaagaag cgctggagct ggcgaacgat acgcaatatg gcctgggcgc
     2641 gggcgtctgg agccgcaacg gtaatctggc ctataagatg gggcgcggca tacaggctgg
     2701 gcgcgtgtgg accaactgtt atcacgctta cccggcacat gctgcgtttg gtggctacaa
     2761 acaatcaggt atcggtcgcg aaacccacaa gatgatgctg gagcattacc agcaaaccaa
     2821 gtgcctgctg gtgagctact cggataaacc gttggggctg ttctgatata agaagctggt
     2881 cgcattgggt attcattgcc tgatgcgacg cttacgcgtc ttatcatgcc tacgggaacc
     2941 tgaccgtagg ccggataagg cgtacacgcc gcatccggca taaacaaagc gcacgttgtt
     3001 acaatttacg ttttaaacct acgtgcgcaa taacccagac tacgccaaca gaagtgcgtt
     3061 tctggggcgt gttttgtcag gagtttaatc tcgcgtttcg atggtgagcg ggcgaaccag
     3121 gccgtaaatc accattggtg atccgaggcc aatcagcaat aaacggtaga taattgaagg
     3181 tttaatacgc ttaatctgta ctgtggatgt tttactggag ttattcatta gtttggccaa
     3241 tagttccact atttcaatat gataaacctc gtcctgttga aaaaccagac agcacatttc
     3301 agaaagtata taaaaaacaa tatgtcgtta tataaataag gaaattgcac tgcatcatgg
     3361 catttcataa tatgcgttaa ttgttctttt ttatattctt tgagggaaat atgttcctgg
     3421 actattttgc actgggagtg cttatttttg tatttctggt gatattctat gggattatta
     3481 ttttacatga tattccctac ctgattgcta aaaaacgtaa tcatcctcat gccgacgcca
     3541 tt
//
LOCUS       DAZOZX010000241         3527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZOZX010000241 DAZOZX010000000
VERSION     DAZOZX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.246"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHUPT_004045"
     CDS             339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHUPT_004045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0821371.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1394..2518
                     /locus_tag="ACHUPT_004046"
     CDS             1394..2518
                     /locus_tag="ACHUPT_004046"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0821372.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHUPT_004047"
     CDS             2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHUPT_004047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0821373.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          937 a          756 c          848 g          986 t
ORIGIN      
        1 tttgacttaa atagcaattt acccgccaat tcttgttgaa catctatact ttttttgtat
       61 ttttatgcga ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga
      121 aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg
      181 cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc
      241 agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct
      301 tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa
      361 ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt
      421 ctctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc
      481 cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa
      541 aagcctcaga aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt
      601 atgaccttat cgtcaatctt accgatcaat ggatggttgc tatactggtt cgcttattaa
      661 atgctcgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa
      721 gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc
      781 tgacaccatt gggacttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa
      841 gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat tatgtggtta
      901 tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga
      961 ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca gataaagacg
     1021 atctggcctg cgtcaatgaa attgcgcagg gatgccagac gccaccagta acggcgctgg
     1081 ctggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg
     1141 gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt
     1201 ttggcgcaac agatcatatt ttctggcgtc cctggtcaaa taacatgatt caattctggg
     1261 cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt
     1321 cggttattcc ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga
     1381 caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt
     1441 gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt
     1501 ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaagtgatca aagtgccggt
     1561 taaatcgcat accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt
     1621 acgcgaacat cccgtcgata aagtcgttgg attcaacaaa atgccggggc tggacgttta
     1681 ttatgccgct gatgtttgtt atgccgagaa agtagcgcag gaaaaaggct ttttctatcg
     1741 cctgacgtca cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg aacagggcaa
     1801 gccgacacag ctgctgatgc tgacagataa gcaaatcgcc gatttccaga aacattatca
     1861 gactgaagcg gagcgttttc atattctgcc accggggatt tatcctgatc gtaaatatag
     1921 ccagcagcca gccaatagcc gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca
     1981 atatttattg ttgcaggtcg gttcagactt cacgcgtaaa ggtgccgatc gttccattga
     2041 agcacttgct tcgttaccgg attcgctgcg ccacaacaca ttgctatatg ttgttgggca
     2101 ggataaaccg cgaaaatttg aggcactggc agaaaaacgc ggcgtgcgca gtaatgttca
     2161 cttcttctcg gggcgcaacg atgtctcgga attaatggcg gcggcggatt tattactgca
     2221 tcctgcctac caggaagcgg cgggaattgt gctgctggaa gcgattactg caggattacc
     2281 ggtactaaca actgccgttt gtggctatgc gcattatatt gtcgacgcta attgcggcga
     2341 ggctattgct gagccattcc gccaggaaac attgaatgag attttacgca aagcgttaac
     2401 gcaatcttca ttgcgccagg cttgggcgga aaatgcgcga cattatgctg atacacaaga
     2461 tttatacagt ctgccagaga aagcggcgga tatcataacg ggtggtctgg atggttgaac
     2521 ttaaagagcc gtttgccacg ttatggcgcg gcaaagatcc ttttgaggaa gttaaaacct
     2581 tgcagggtga ggtatttcgt gaactggaaa ctcgccgtac tctgcgcttt gaaatggcgg
     2641 gaaaaagcta ttttctcaaa tggcatcgcg gcacgaccct gaaagagata atcaaaaatt
     2701 tactctcatt gcggatgcca gtattaggcg cagaccgcga atggaatgcg attcatcgac
     2761 tgcgggatgt cggcgttgat actatgtatg gggtggcatt cggcgaaaaa ggcatgaatc
     2821 cgctgaccag aacctcgttt attattaccg aagatctgac accaaccatc agtctggaag
     2881 attactgtgc tgactgggcg accaacccac cagatgttcg cgtaaagcgt atgcttatta
     2941 agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg cattaaccat cgtgactgtt
     3001 atatctgtca tttcctgctg cacttgcctt tttccggtaa ggaagaggag ttaaaaattt
     3061 cggtaattga cctacaccgg gcgcagcttc gcacgcgcgt tccaggtcgt tggcgcgata
     3121 aagatcttat agggctttat ttttcttcaa tgaatattgg cctgactcag cgggatatct
     3181 ggcgctttat gaaggtgtat tttgccgccc cgcttaaaga cattctcaag caggaacaag
     3241 gactgctgtc gcaagcagaa gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat
     3301 cgttgtaacc cgtaacttat ttttgccaaa attttggata cagaataaat atgtctcaac
     3361 tcaatgatag tgacatcatc ctttttgagt ataattttca ttatcaaaat ataagatcta
     3421 aaaatactct tgatatagca tttggtattg acagaaattt tctttttgga tgtggtgtag
     3481 ccatcgcatc tattctatta aacaatagag aaatctcttg tgaattt
//
LOCUS       DAZOZX010000242         3497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZOZX010000242 DAZOZX010000000
VERSION     DAZOZX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.247"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..312
                     /locus_tag="ACHUPT_004048"
     CDS             <1..312
                     /locus_tag="ACHUPT_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR0821374.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            469..1218
                     /locus_tag="ACHUPT_004049"
     CDS             469..1218
                     /locus_tag="ACHUPT_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0821375.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1240..1806
                     /gene="yieF"
                     /locus_tag="ACHUPT_004050"
                     /gene_synonym="chrR"
     CDS             1240..1806
                     /gene="yieF"
                     /locus_tag="ACHUPT_004050"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR0821376.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="ACHUPT_004051"
     CDS             complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="ACHUPT_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0821377.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3362..>3497
                     /locus_tag="ACHUPT_004052"
     CDS             3362..>3497
                     /locus_tag="ACHUPT_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0821378.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          961 a          908 c          865 g          763 t
ORIGIN      
        1 gcactggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
       61 gaattcgagc agtcactgtt tatggcagcg caacccgaca atctgctact ggcgaccgcg
      121 ccgcgctact gtcagtacta caatcaactc catcaactgc cgttggttgc tcttcctctc
      181 ccgtttgacg aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa
      241 cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg
      301 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag
      361 ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
      421 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
      481 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
      541 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
      601 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
      661 ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat
      721 gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
      781 ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
      841 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
      901 attacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
      961 gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
     1021 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa
     1081 aagctcagtg tctgggagtt agatggcaaa cacagctgga aaagcctgcc ggatattcac
     1141 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
     1201 tctttttcgc caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg
     1261 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
     1321 ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc
     1381 ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg
     1441 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
     1501 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc
     1561 ggtaaaccgg tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
     1621 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt
     1681 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt
     1741 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
     1801 atctaaataa aaaacccgcc agtaatcatg catggcgggt ttttaatgcg ctatcgattt
     1861 taatgagcgt cgataaatac aatcttcagg ataaacagca gcgcaacgat gattacgcac
     1921 gggctaaggt cacgcagacg cccggtacca atcttcatca cgcagtagga gataaagccc
     1981 agcgcaatac cttcggtaat cgaaaagctg aacggcatca tcacggcggt aataaacgcc
     2041 ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg
     2101 ccaacgtaga tcaacgcgcc agctgcagcg tagtctggca ccattcccgc cagcggcgac
     2161 agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg
     2221 ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac
     2281 gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaatttc
     2341 cccttctcat ccgccagacc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
     2401 aacaagttga ccaacatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg
     2461 gctaaatcta catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg
     2521 tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc
     2581 gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga aaaagccgag gatacccaga
     2641 agtacgctgt gagaagtcag attaccgatg ctcaccagcg tttccgagtt agcgacaatc
     2701 acacctgcgt ttttcagccc catcatgccg ataaacaggc cgataccgct ggtaataccc
     2761 acacgcagac ttaccggaat gttagcaatc atccagtaac gaacgcggaa aatggtcaaa
     2821 agaagcagtc cgacagcgcc ccagaagatt gcgcccatac cgatctgcca cggcagcccc
     2881 attgcctgga caaccacaaa agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc
     2941 accggaaggt ttgcaaacaa tcccatcata atgctaccga aagcagcaat cagacaggtt
     3001 gttacgaaga cggcactggt gtccatgcca gcaacgccca gaatttgtgg gttaacaaat
     3061 acgatgtaga ccatcgtcag gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta
     3121 gtaccatgtt cgcgcagttt aaaaacacgt tccagcatcc tctgaccaga agtctgggtg
     3181 gtatgttgat gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
     3241 accaaaatgc gacaataggc gcgtttgtga gagacttttt tattggattt acttatacgg
     3301 caacgattgc gttgcgcaaa tcggctctac gaaaacgtta aactgattaa aaaggaaagg
     3361 catgtcccgg atagaagcgg tatttttcga ctgcgacggt acgctggtcg acagtgaagt
     3421 catttgctcc cgcgcatatg taacgatgtt tcaggaattt ggtattacgc tcgatcctga
     3481 agaggtattc aaacgtt
//
LOCUS       DAZOZX010000243         3456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZOZX010000243 DAZOZX010000000
VERSION     DAZOZX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.248"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(226..429)
                     /locus_tag="ACHUPT_004053"
     CDS             complement(226..429)
                     /locus_tag="ACHUPT_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821379.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHUPT_004054"
     CDS             complement(431..586)
                     /locus_tag="ACHUPT_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0821380.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHUPT_004055"
     CDS             complement(789..1196)
                     /locus_tag="ACHUPT_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821381.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHUPT_004056"
     CDS             1273..1500
                     /locus_tag="ACHUPT_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0821382.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHUPT_004057"
     CDS             1484..1906
                     /locus_tag="ACHUPT_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0821383.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHUPT_004058"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHUPT_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACHUPT_004059"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACHUPT_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZOZX010000244         3424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZOZX010000244 DAZOZX010000000
VERSION     DAZOZX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.249"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            87..803
                     /gene="nanC"
                     /locus_tag="ACHUPT_004060"
     CDS             87..803
                     /gene="nanC"
                     /locus_tag="ACHUPT_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0821384.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            823..1929
                     /gene="nanM"
                     /locus_tag="ACHUPT_004061"
     CDS             823..1929
                     /gene="nanM"
                     /locus_tag="ACHUPT_004061"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0821385.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1994..2974
                     /locus_tag="ACHUPT_004062"
     CDS             1994..2974
                     /locus_tag="ACHUPT_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0821386.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3084..3289
                     /locus_tag="ACHUPT_004063"
                     /pseudo
     CDS             3084..3289
                     /locus_tag="ACHUPT_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1002 a          727 c          802 g          893 t
ORIGIN      
        1 tggcaaaaaa atattacaca acataaatgc taattgttta tgcgggcttt gtattgcttt
       61 ctgtatccta caaatgagtg aaatttatga aaaaggctaa aatactttct ggcgtattat
      121 tactgtgctt ttcgtcccca ttaatttctc aggctgcgac actggacgta cgtggtggat
      181 atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga tggcaaaatg
      241 gatggtgggc aagcatggaa agtaatacct ggaataccat tcatgataat aaaaaggaaa
      301 atgccgcact caatgatgtt caggttgaag ttaattacgc aattaaactt gatgatcaat
      361 ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc tacggaccct
      421 acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc tatcgttacg
      481 actggaaagc ttaccgacaa caagacttat ccggtgatat gtctcgtgat aacgttcatc
      541 gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca tggcaaacga
      601 cgctatacag caaacagaat gattatcgct atgcaaacca caagaaatgg gcgacggaaa
      661 atgcctttgt tctacaatac catatgacgc ccgatattac gccatacata gaatatgact
      721 accttgaccg ccagggtgtt tacaacggca gagataattt atcggaaaac agttatcgca
      781 ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa aactataatg
      841 gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct
      901 gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat tggtttaggt
      961 agcgcaggta cagcatggta caagctagat acacaggcca aagataaaaa atggactgcg
     1021 ttagctgcat tccctggtgg accgagagag caagcaacct ctgcatttat tgatggcaat
     1081 ctgtatgtgt ttggcggcat tggcaaaaac agcgagggat tgactcaggt atttaatgac
     1141 gtacacaaat acaaccccaa aaccaatagc tgggttaaat tgatgtcgca cgcgccgatg
     1201 ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg cttatgttac tggcggtgtt
     1261 aaccagaata tcttcaatgg ctattttgaa gatctcaacg aggctggaaa agattcaacc
     1321 gctatagata aaatcaacgc ccactatttt gacaaaaaag cagaagatta tttcttcaat
     1381 aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg cgaatcgccc
     1441 tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg gcttattaat
     1501 ggcgaagcca aaccaggatt gcgaacggat gccgtatttg aacttgattt caccggtaat
     1561 aatttaaaat ggaataagct tgatcccgtc tcatcaccag atggcgtcgc tggcggtttt
     1621 gcggggataa gcaatgattc tcttatattt gctggagggg ccggattcaa aggttcacga
     1681 gaaaattacc agaacggtaa gaactatgcg catgaaggcc tgaaaaaatc atatagcact
     1741 gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg
     1801 gcctacggag tatcattgcc ctggaataat agtttattaa ttattggcgg tgaaactgca
     1861 ggcggcaaag cggtgacgga ttcagtttta atttctgtga aggataataa agtcacagta
     1921 caaaactaac gcttcagggc cccggtaaag gggccctgct atcaatttgt catttgtatt
     1981 acgcgaaaat tatatgaacg caataatatc acccgattat tactatgttc ttaccgttgc
     2041 tggtcagtct aatgccatgg cgtatggcga aggactgcca ttaccggaca gggaagatgc
     2101 gcctcattcc agaattaaac aattagcgag atttgcgcat acgcatcccg gaggcccgcc
     2161 atgtcacttt aacgacatta ttccactgac tcactgccca cacgatgttc aggatatgca
     2221 gggttatcac catcctctgg caacgaatca tcaaacacag tacggcaccg ttggccaggc
     2281 actgcatatt gcacggaaat tactgccctt cattcctgat aatgcaggga ttcttatcgt
     2341 tccgtgttgc cgtggcggat cggcttttac cgcgggcagc gaagggacat attcagaacg
     2401 gcacggagcc agccatgatg cttgtcgttg gggaacggat actccgctgt accaggattt
     2461 agtcagcaga acgcgagccg cactggcaaa aaatccgcag aacaaattcc tcggcgtatg
     2521 ctggatgcaa ggcgaatttg acttaatgac cagtgactac gcgtcacacc ctcaacactt
     2581 taatcatatg gttgaagcct ttcgtaggga tctaaaacaa taccattctc agcttaataa
     2641 tattactgac gcgccgtggt tttgcggcga taccacctgg tactggaaag aaaatttccc
     2701 gcattcgtat gaagctattt atggcaatta tcaaaataat gttttagcca atattatttt
     2761 cgtcgacttc cagcaacaag gtgaaagagg actgacgaac gcgcctgatg aagacccgga
     2821 cgatttaagc acgggatatt acggttcagc gtaccggtca ccggagaact ggacgacggc
     2881 actgcgaagc agtcatttca gcacggcagc ccgtcggggg attatttctg acagatttgt
     2941 agaagcaatt ttgcagtttt ggcgcgaaag gtgagcgggg agtttattca tgggggatgt
     3001 ccttgttcgt tctcagagta gacaacaaaa aacaaatgag atcaaggtaa tatctcttca
     3061 cttgcgggct cgaacagcag gcaattgcca atataataat ctggcacaag ggtgaaaaac
     3121 gcgcaccgca taaaccgttg ttattgctta cagattagcc ggatgcctta atagacatcc
     3181 gcgccttttc gattatggct cggaaatcta cgaacatcta cacagtttac tggaacgttt
     3241 tgggccacag cgttcgcagc accgtcctga tatgccgttc tggcgattat aagtcgacgg
     3301 attctggcaa atagacgatg atgatatgct taatgatgtt caaagaaatt aggccattat
     3361 tatattattg cttgcatttt ctttcattcc taaaagcacc cgccattaat cccaggcgca
     3421 ttat
//
LOCUS       DAZOZX010000245         3414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZOZX010000245 DAZOZX010000000
VERSION     DAZOZX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.250"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..238
                     /locus_tag="ACHUPT_004064"
     CDS             <1..238
                     /locus_tag="ACHUPT_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR0821387.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="ACHUPT_004065"
     CDS             248..886
                     /locus_tag="ACHUPT_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0821388.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="ACHUPT_004066"
     CDS             889..2052
                     /locus_tag="ACHUPT_004066"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0821389.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="ACHUPT_004067"
     CDS             2136..>3414
                     /locus_tag="ACHUPT_004067"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0821390.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAZOZX010000246         3412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZOZX010000246 DAZOZX010000000
VERSION     DAZOZX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.251"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="ACHUPT_004068"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="ACHUPT_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0821391.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /gene="dfrA1"
                     /locus_tag="ACHUPT_004069"
     CDS             complement(687..1160)
                     /gene="dfrA1"
                     /locus_tag="ACHUPT_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0821392.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2469
                     /gene="intI2"
                     /locus_tag="ACHUPT_004070"
                     /pseudo
     CDS             1492..2469
                     /gene="intI2"
                     /locus_tag="ACHUPT_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(2657..2896)
                     /locus_tag="ACHUPT_004071"
     CDS             complement(2657..2896)
                     /locus_tag="ACHUPT_004071"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0821393.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          943 a          756 c          720 g          993 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag cttaggtgtt
     2701 ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg cgtttctgaa
     2761 gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct tggagtaaca
     2821 agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga tcggagttat
     2881 agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt attattgtag
     2941 ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg tatattaatt
     3001 tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat caagataact
     3061 gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat acgggatcac
     3121 tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact gatgaaagga
     3181 ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct agattagtta
     3241 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3301 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3361 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagctgc cg
//
LOCUS       DAZOZX010000247         3407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZOZX010000247 DAZOZX010000000
VERSION     DAZOZX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.252"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            233..568
                     /locus_tag="ACHUPT_004072"
     CDS             233..568
                     /locus_tag="ACHUPT_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0821394.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            726..1169
                     /gene="yjdN"
                     /locus_tag="ACHUPT_004073"
     CDS             726..1169
                     /gene="yjdN"
                     /locus_tag="ACHUPT_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR0821395.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1302..2090
                     /gene="phnC"
                     /locus_tag="ACHUPT_004074"
     CDS             1302..2090
                     /gene="phnC"
                     /locus_tag="ACHUPT_004074"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0821396.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            2115..3131
                     /gene="phnD"
                     /locus_tag="ACHUPT_004075"
     CDS             2115..3131
                     /gene="phnD"
                     /locus_tag="ACHUPT_004075"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0821397.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3237..>3407
                     /locus_tag="ACHUPT_004076"
     CDS             3237..>3407
                     /locus_tag="ACHUPT_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR0821398.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
BASE COUNT          878 a          943 c          863 g          723 t
ORIGIN      
        1 aaaatatcat gtgaataaaa aaagaacaag tagagcatta acattatctt aaataataaa
       61 tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg atcgtcgtct
      121 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
      181 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
      241 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
      301 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
      361 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
      421 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
      481 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
      541 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
      601 tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg tcttatcagg
      661 cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca
      721 ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac gcgattgcct
      781 attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccga
      841 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
      901 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt
      961 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
     1021 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
     1081 ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
     1141 atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc
     1201 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
     1261 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg
     1321 tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt gatctgaaca
     1381 ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt
     1441 tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat atcgagctac
     1501 tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa agccgcgcca
     1561 acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg
     1621 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
     1681 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
     1741 atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt gcccgtgcgc
     1801 tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg gacccggaat
     1861 cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg
     1921 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
     1981 gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa cgttttgacc
     2041 atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga tatccccatc
     2101 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
     2161 gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg
     2221 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
     2281 atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac tacgcgggca
     2341 ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac ctgtcggcga
     2401 tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg gcggatggat
     2461 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg
     2521 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
     2581 gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc agcgacttca
     2641 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaagtgg
     2701 atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
     2761 ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg atcgtctggc
     2821 gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg aactacggaa
     2881 aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca
     2941 gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata caaagcgtga
     3001 aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag attcaggcgc
     3061 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
     3121 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgtagg
     3181 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
     3241 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
     3301 ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
     3361 aagacggcgg caacatggca accttcgcgg taatgactcc aacttac
//
LOCUS       DAZOZX010000248         3373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZOZX010000248 DAZOZX010000000
VERSION     DAZOZX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.253"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1202
                     /locus_tag="ACHUPT_004077"
     CDS             <1..1202
                     /locus_tag="ACHUPT_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR0821399.1"
                     /translation="TKDGQRLRNMIMSFNADIVARSPKKKPTFFPEQIEGYEYMYGGN
                     DDYPYYLQNRTDENGNDLPIGPISYMENPEVPQANAYMLEAATNAVKEVASLGVDAQA
                     ANGQVAFDTVNQLNMRADLETYVFQDNLATAMRRDGEIYASMVNDIYDVPRHVTLTLE
                     DGSEKDVQLYAQVVDYQSGNVVTLNDIRGRYECYTDVGPSFQSMKEQNRAEIQELLTK
                     VPQGTPEFQMLMLQYFTLLDGKGVEMMREYANKQLVMMGLKKPETPEEMEMVQQAQQQ
                     PQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMD
                     LDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQTHSQRMDFANLMRQVQIPSG
                     GVAETPQ"
     gene            1216..2127
                     /locus_tag="ACHUPT_004078"
     CDS             1216..2127
                     /locus_tag="ACHUPT_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR0821400.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            2127..>3373
                     /locus_tag="ACHUPT_004079"
     CDS             2127..>3373
                     /locus_tag="ACHUPT_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR0821401.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACA"
BASE COUNT          927 a          872 c          872 g          702 t
ORIGIN      
        1 tgacgaaaga cggtcaacgc cttcgtaaca tgattatgtc gttcaacgcc gatattgttg
       61 ctcggtcacc gaagaagaaa ccgaccttct tccctgagca aatcgaaggc tacgaataca
      121 tgtacggcgg aaatgatgac tatccgtact atctgcagaa caggactgat gaaaacggta
      181 acgacctgcc gattggtcca atctcctaca tggaaaaccc tgaagtgccg caagccaacg
      241 cttacatgct tgaggctgcc accaacgcag tgaaagaagt ggctagtctt ggcgtggatg
      301 cgcaggcggc aaatggtcag gtcgcttttg ataccgtcaa tcaactgaac atgcgggcag
      361 accttgagac atacgtgttt caggataacc tggctaccgc aatgcgacgt gatggcgaga
      421 tttatgcctc aatggtcaac gatatttatg acgttcctcg tcatgtaacg ctgacacttg
      481 aagatggaag cgagaaagac gttcaactct atgcgcaagt tgtcgattac cagtccggca
      541 atgtggtcac actcaacgac attcgcggtc gatatgagtg ctatacagac gttgggccat
      601 ccttccagag tatgaaggaa cagaaccgcg cagagattca ggagttgctc accaaggttc
      661 cgcaaggtac tccagagttc cagatgctga tgctgcaata cttcacgctg cttgacggta
      721 aaggcgtcga gatgatgcga gagtacgcga acaagcaact ggtgatgatg gggctgaaga
      781 aaccagaaac acctgaagag atggagatgg tgcagcaggc acaacaacag ccgcagcagc
      841 catcagcaga gcaaattcag gcgcagggta tccttctgca aggtcaggct gaattgctca
      901 aggcagagaa ccaacaggcg cagattcagg ttgaagctgc caaggttgaa gcccaaaacc
      961 aactcaacgc cgcgaagatt gcagaaatat tcaacaatat ggacctcgac aagcaggcag
     1021 aactgcgtga gtacctcagg ctcgtaggtc aattccagca acagcgcagc aaagatgctc
     1081 gtgctaacgc tgagctgctt cttaaagatg cagaccagac tcattcacaa cgcatggatt
     1141 tcgcgaatct tatgcgtcaa gttcaaatcc cctccggcgg agtagccgag acacctcaat
     1201 aagagagagt taatcatgga ccaaaccacc gacattcagg cttctgaaga attaaccctg
     1261 cccggcaatc atgcagcggc atctgctgat ggcttagttg tcgataatgc caacgacagc
     1321 gcaggtcagg aagaaggctt tgagattgtc cggaaagacg atgagaaacc aaaacaagac
     1381 ccggcaacta atgctgaatt tgcccgtcgc cgcatcgaac gcaaacgcca gcgtgagctt
     1441 gagcagcaga tggaagcggt taagcgtgga gagttgccgg agcacctgcg ggtgaaccct
     1501 gagttaccaa aacaaccaga ccctaacgat tatctttccg aagatgcact ggctaagtac
     1561 gactatgacc agagccgcgc actggctgcc ttccagcagg caaacagtga atggcagatc
     1621 aaggctatgg acgcacgaag ccaggctgtc gccgagcagg gtcgcaaaac tcaggagttc
     1681 acccagcaat cagcgcaata cgtcgaggcg gcccgtaagc actacgaggc agcggaaaag
     1741 ctcaatatcc ctgactatca ggagaaagag gatgcattca tgcaactggt gccgccagca
     1801 gtcggtgccg acatcatgcg cctcttcccg gagaaatccg ccgctctcat gtatcacctt
     1861 ggtgctaatc ctgagaaaac acgccagttg ctggcgatgg acgggcaatc cgcgctgatt
     1921 gaactcactc gactgtcaga acgtttaact ctcaagcctc gagccaagcc tgtttcagaa
     1981 gccccgttac ctgatgaacc cattcaggga catgctgttg ctgcaaatat ctctgcgatt
     2041 gaaaagcaga tggaggcggc agcaaacaaa ggggatgtag agacgtaccg caagctcaag
     2101 gcgcaactga ataaaggaat tcgataatgg cattaaatga aggtcaactg gtcacgtatg
     2161 ctctggatga aatcatcgga accgtccaga acctgacgcc aatggcgtca aaagtgacaa
     2221 aatacccccc tccggcagaa tccatgcagc gttcaagcaa caccgtgtgg atgcctgttg
     2281 agcaggaagc gccaacccag actggctggg atttaactgg caacgcaacc gggattctgg
     2341 aactctccgt gaagtgcaac atgggcgatc cggataacga tttcttcgag cttcgtgcag
     2401 atgacctgcg tgatgagcgt tcttaccgtc gccgcatcca ggcatccgcc aaaaaactgg
     2461 cgaataacat tgagtcagcg attgccaaac aggcaactga aatgggctcg cttgttgttc
     2521 acgatacccg cgcaattggt ccatcgactg gcctgcctgg ctgggatttt gtgtctgatg
     2581 cagagcgact gatgttctcc cgtgagctaa accgcgacat gggcatcagt tacttcctga
     2641 accctgacga ttaccgcaaa gcaggccgca acctggtaga tggtgacatc ttcgggcgcg
     2701 ttcctgaaga agcatatcga aacggtacta ttcagcgtca gattgctggc tttgatgaaa
     2761 ttcttcgctc accgaaactt ccggcagtta ccaagtcaac cgcgactggt gtaactgttt
     2821 ctggtgcgca gaagtttaag ccgcaggcat acacccttga taccgatggt aacaaagaga
     2881 acgtcgacaa ccgtgttgca acggtgaccg tatcctccac caccggattt aagcgcggcg
     2941 acaaaatcag cttcattggt gtgaagttcc tgtctcagat ggcgaagaat gtgctgactg
     3001 atgacgcgac tttctcaatc acccgtgtga tcgatggtac tcacatcgaa atcacgccga
     3061 agccgattgc gctggatgac gcgtcactga caaaagaaga gaaggcttac gctaacgtaa
     3121 acacctctct tgctgatacc actccggtaa acgttctgaa cgtggcaaca accaccgcta
     3181 acgtgttctg ggctgatgac tcaatccgcc tgctgtctca gccgatcccg gtaacccatg
     3241 aactgtttgc tggcatgaaa acgtcttcct tcagcattcc aggtattggt gttaacggca
     3301 tcttcgcaac gcagggtgat atcaacactc tgtctggtaa gtgccgtatt gctgtgtggt
     3361 attcagcatg tgc
//
LOCUS       DAZOZX010000249         3348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZOZX010000249 DAZOZX010000000
VERSION     DAZOZX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.254"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            19..897
                     /locus_tag="ACHUPT_004080"
     CDS             19..897
                     /locus_tag="ACHUPT_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0821402.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHUPT_004081"
     CDS             894..2285
                     /locus_tag="ACHUPT_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0821403.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHUPT_004082"
     CDS             2282..2539
                     /locus_tag="ACHUPT_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821404.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHUPT_004083"
     CDS             2634..2834
                     /locus_tag="ACHUPT_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR0821405.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHUPT_004084"
     CDS             2827..3207
                     /locus_tag="ACHUPT_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0821406.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAZOZX010000250         3344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZOZX010000250 DAZOZX010000000
VERSION     DAZOZX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.255"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(2..298)
                     /locus_tag="ACHUPT_004085"
     CDS             complement(2..298)
                     /locus_tag="ACHUPT_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR0821407.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACHUPT_004086"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACHUPT_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0821408.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHUPT_004087"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHUPT_004087"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0821409.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACHUPT_004088"
     CDS             complement(1784..2323)
                     /locus_tag="ACHUPT_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0821410.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACHUPT_004089"
     CDS             complement(2353..3096)
                     /locus_tag="ACHUPT_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0821411.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="ACHUPT_004090"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="ACHUPT_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT          962 a          826 c          795 g          761 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccag
//
LOCUS       DAZOZX010000251         3336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZOZX010000251 DAZOZX010000000
VERSION     DAZOZX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.256"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            274..549
                     /locus_tag="ACHUPT_004091"
     CDS             274..549
                     /locus_tag="ACHUPT_004091"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR0821412.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
     gene            747..2111
                     /locus_tag="ACHUPT_004092"
     CDS             747..2111
                     /locus_tag="ACHUPT_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0821413.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2161..2370
                     /locus_tag="ACHUPT_004093"
                     /pseudo
     CDS             2161..2370
                     /locus_tag="ACHUPT_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2464..2655)
                     /locus_tag="ACHUPT_004094"
                     /pseudo
     CDS             complement(2464..2655)
                     /locus_tag="ACHUPT_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2696..3193)
                     /locus_tag="ACHUPT_004095"
                     /pseudo
     CDS             complement(2696..3193)
                     /locus_tag="ACHUPT_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          819 a          738 c          790 g          989 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     2041 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg
     2101 aaaaagagta attttctcag gaaaaacata aaattactga ctaaatattt attttttgaa
     2161 tcgcagggca ttccggataa ggttgatatc gtgtcacggc tgaagaccta tggatacagt
     2221 atttctggtg tggagacgga tgacggatat aaggcgcttg tccgcgcttt tcagcttcat
     2281 ttccggcaga aaaattatga cggcataatg gatgctgaaa ccgctgccat cctgtatgca
     2341 ttgctggaga agtatttccc gggtaaataa ggagtgagtc gtcagagctg cgatatatct
     2401 ccttcctcca tgggctgtct cttatacaca aatccccacc cggggatttg tgatgtcaga
     2461 tgtccccagt aagactatgt tttcattgcg ctctatgaag ctgagtgagc gtaacgactg
     2521 gagctgcttc tgcggtgctc cggtggcgaa cgtgaagtca tactcttcga acattttcac
     2581 cgccgggaag gctgccattc gggtatacat cgcctgttta cgttgatgac gtgccagttt
     2641 ttcttcatga agcatgatgc tccaagtaaa cgttcaccga acaccgtatt gacgattatc
     2701 ctgatggtag caccaccttc catggcagga ggtcgtcaac tcggttggaa ggccattccg
     2761 gcagtacgct cagaatatgg cgcagatacg cttccggatc gataccgttc agacggcagg
     2821 tgccgatcag cccgtacagc agtgcaccac gctcgccgcc gtgatcgcta ccgaagaaca
     2881 cgtaattttt ctttccgaga cagactgcac gaagcgctct ttccgctgtg ttattgtccg
     2941 cctccgccag accgtcatca ctgtaataac agagggcgtc ccactgattc agtacatagc
     3001 tgaatgcttc gcccagtctg gattttttcg acagcgtgcc attcttctcc accatccatt
     3061 catgcagcga cgtcagtaac gctttgcttc gctgctgcct gactgcaaga cactctgact
     3121 ctggtaatcc ccgtatttca tcctcgatgg cgtacagttc actgattcgc ttcagggctt
     3181 cttctgccgt cgcacgcaag taatccgggg cccgctatgc cgcgctctat cgggcgggac
     3241 ggggctggcg ctttgaaccg ccccgggttt cctggagagt gttttatctg tgaactcagg
     3301 ctgccagatc atcgtttccg atggaagcat aataag
//
LOCUS       DAZOZX010000252         3325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZOZX010000252 DAZOZX010000000
VERSION     DAZOZX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.257"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHUPT_004096"
     CDS             <1..208
                     /locus_tag="ACHUPT_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821414.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACHUPT_004097"
     CDS             269..457
                     /locus_tag="ACHUPT_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821415.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACHUPT_004098"
     CDS             454..1281
                     /locus_tag="ACHUPT_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0821416.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACHUPT_004099"
     CDS             1325..2074
                     /locus_tag="ACHUPT_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0821417.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACHUPT_004100"
     CDS             2071..2640
                     /locus_tag="ACHUPT_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821418.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACHUPT_004101"
     CDS             2637..2759
                     /locus_tag="ACHUPT_004101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821419.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACHUPT_004102"
     CDS             2764..3006
                     /locus_tag="ACHUPT_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR0821420.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACHUPT_004103"
     CDS             3010..3156
                     /locus_tag="ACHUPT_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821421.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHUPT_004104"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHUPT_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR0821422.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZOZX010000253         3314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZOZX010000253 DAZOZX010000000
VERSION     DAZOZX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.258"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..98
                     /locus_tag="ACHUPT_004105"
     CDS             <1..98
                     /locus_tag="ACHUPT_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0821423.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACHUPT_004106"
     CDS             156..512
                     /locus_tag="ACHUPT_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821424.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="ACHUPT_004107"
     CDS             605..823
                     /locus_tag="ACHUPT_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0821425.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="ACHUPT_004108"
     CDS             825..1190
                     /locus_tag="ACHUPT_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0821426.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="ACHUPT_004109"
     CDS             1187..1852
                     /locus_tag="ACHUPT_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821427.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="ACHUPT_004110"
     CDS             1852..2217
                     /locus_tag="ACHUPT_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0821428.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2672..2827
                     /gene="hokD"
                     /locus_tag="ACHUPT_004111"
     CDS             2672..2827
                     /gene="hokD"
                     /locus_tag="ACHUPT_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0821429.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3103..>3314
                     /locus_tag="ACHUPT_004112"
     CDS             3103..>3314
                     /locus_tag="ACHUPT_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821430.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          960 a          673 c          855 g          826 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcagacag agaaaagccc cagttaacat tacgcgtcct
     2581 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     2641 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     2701 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
     2761 cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcacagctt acgaacctga
     2821 ggagtaagag accaggcggg ggagaaatcc ctcgccacct ctcatgtgtc aggcatcctc
     2881 aacgcacccg cactaaaccc gcttcggcgg gttttttgtt gcgtgctgaa tacgcagggt
     2941 gaaaaataac catatatttg attatataca caacaaaaaa taaaagtcat tgtacttgca
     3001 cattaaacaa tcaaatatac ggcgtgaaat aaatatttat cagattaata tttttgtctc
     3061 tatgtggata taaccgtttg tacttataaa cctgggggca tcgtggaaaa aataaagaaa
     3121 ctatttagta gcaaatacgc agtcatacgt cgtgatgacc tgtcagttat agtcgaaatg
     3181 gattacttcc ctgaaacccc aaaatcaata atgtatcgta atggtcgaaa ggcaattttt
     3241 ttaccgatga gggtaagtga cattatggga aatgataaac tgctggatga attgcgagtc
     3301 agagcatcct gtta
//
LOCUS       DAZOZX010000254         3310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZOZX010000254 DAZOZX010000000
VERSION     DAZOZX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.259"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            95..2248
                     /locus_tag="ACHUPT_004113"
     CDS             95..2248
                     /locus_tag="ACHUPT_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR0821431.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..>3310
                     /locus_tag="ACHUPT_004114"
     CDS             2301..>3310
                     /locus_tag="ACHUPT_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0821432.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFL"
BASE COUNT         1202 a          591 c          603 g          914 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct
//
LOCUS       DAZOZX010000255         3299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZOZX010000255 DAZOZX010000000
VERSION     DAZOZX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.260"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(139..993)
                     /locus_tag="ACHUPT_004115"
                     /pseudo
     CDS             complement(139..993)
                     /locus_tag="ACHUPT_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="ACHUPT_004116"
     CDS             1101..1379
                     /locus_tag="ACHUPT_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821433.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="ACHUPT_004117"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="ACHUPT_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0821434.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="ACHUPT_004118"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="ACHUPT_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0821435.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          940 a          731 c          703 g          925 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtggttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaa
//
LOCUS       DAZOZX010000256         3284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZOZX010000256 DAZOZX010000000
VERSION     DAZOZX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.261"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(265..1689)
                     /gene="patD"
                     /locus_tag="ACHUPT_004119"
     CDS             complement(265..1689)
                     /gene="patD"
                     /locus_tag="ACHUPT_004119"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0821436.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1711..2505)
                     /locus_tag="ACHUPT_004120"
     CDS             complement(1711..2505)
                     /locus_tag="ACHUPT_004120"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0821437.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2495..2842)
                     /locus_tag="ACHUPT_004121"
                     /pseudo
     CDS             complement(2495..2842)
                     /locus_tag="ACHUPT_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2870..3115
                     /locus_tag="ACHUPT_004122"
                     /pseudo
     CDS             2870..3115
                     /locus_tag="ACHUPT_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          846 a          920 c          788 g          730 t
ORIGIN      
        1 tgaaggtaag ggttttattt ttatcttccg tgaatttgta caattgaact ttcattgtta
       61 atacttctac ttataaagta ttagtttaat aacgatactc taaagcctta tgctaatcaa
      121 ctgtgacagg atgaaaaatc gccatcaaac atttagccta atttttacgc cagttattaa
      181 tgtaatcatt ctaatactta accgccatta aactcagaac ctaaaacgat agtggccgtc
      241 ctataacagc cactaacatc taacttaatg tttaaccatg acgtggcgga cgacggtgta
      301 atcctccagc ccataaagtg acatatcctt gccgtaacca gaaagtttct gcccaccgtg
      361 cggcatttca cttaccagca tgaaatgggt attgacccag gtacaaccat attgcagccg
      421 tgcgctgacg cgatgcgccc tgcccacatc tttcgtccat accgaagatg caagtccgta
      481 ctggctgtca ttcgcccagt tcaccacctg ttcttcgttg tcgaagggcg taacactcac
      541 tactggacca aatacctctt tttgcacgat ggcatcgtcc tgtaatgcgc cagccagcag
      601 cgtcggcgca tagtaatagc cattaccctt gcgcttttca ccgccagtga tcactttgat
      661 gtgccctgtc gctttcgcct cttctactgc cttgctgacg cgttcgagat gcgccagcga
      721 gcttaaaggt ccaagctccg tagactcgtc atccggcgca ccagatttta acgttgccac
      781 cgcagcaccc agtttttcca ccagcgtatc gtaaatgcct ttttgcgcgt agatccgaca
      841 agccgcagta caatcctgtc cagcattgta atagccaaat gtacgtacac cttcgaccac
      901 tgcttcaata tccgcatcat caaaaacaat cactggcgct ttgccaccaa gttccatatg
      961 agtacgctta atggacgacg cggtatggct gatgatgtgc tcgccggtgg cgatagagcc
     1021 cgtcagcgac accatccgca ctttgggatg accggtcagc ggatcaccca ccgttttgcc
     1081 tctgccaaac agtatgttaa tcacgcctgc cgggaagata tctttcgcca gctctgccaa
     1141 cttcaacgcg gtcagcgggg taatttctga tggtttaagc actacgcagt tccctgccgc
     1201 cagcgccgga gcaagtttcc acgcggccat catcagcgga taattccacg gtgcgataga
     1261 agccacgacc cccaacggat cgcgacggat catcgaagta tgaccttcaa gatattcacc
     1321 tgccgccaga ccattcagac agcgcgccgc acccgcgaaa aagcgaaaaa catcgacaat
     1381 cgccgggatt tcatcattga acgcactatg cagcggtttg ccacaattac gggattccag
     1441 ttcggcaaaa acctgaccat tttcttcgat aacatccgcc agtttcagca ggcattccgc
     1501 acgcgctttc ggtgtggttt gcccccattc agcaaatgct gcatctgccg cacgcacagc
     1561 agcatcgacc tgctctgcgg atacctcggc aatctccagt aaaacgtccc ccgttgccgg
     1621 attatagaca ggctgttttt ccccttcgcc gctaacccgt tctccgttaa tcagtaactt
     1681 atgttgcata gcttattcct gtatcagtgg ttattgacca ttgtcgcctt cgcgggttag
     1741 ccaccaggcc cccaggatcg gcaaggttgt taccaacata accagcagtg ccaccacgtt
     1801 ggttaccggt acatcacgcg gtcgcccaag ctgattgagc aaccacaacg gtaacgttcg
     1861 ttcatgacct gccgtaaagg tcgtaacgat gatttcatcg aacgacaagg caaacgccag
     1921 cattcctcct gccagtaacg ccgaactgag attcggcaac actacgtagc ggaaggtttg
     1981 ccagccattg gccccaagat ccattgacgc ctcaaccaga ctccacgagg tgcggcgaaa
     2041 acgggcgatg acattgttaa acaccaccac tacgcaaaaa gtcgcatgac cgaccacgat
     2101 ggtgaaaaat cccggctcca gattgatggt tttaaaggcg gttaatagcg ccagaccagt
     2161 gacaatgccc ggcagcgcaa tgggcagcag caataacagc gaaatggcgt ttttgccgaa
     2221 aaagtctcgt ctccacagcg cggcagctgc cagcgtccct aacaccagcg caattaatgt
     2281 cgccagcgcc gccactttaa gtgacagcgt cacggcatca agaatatcac tacgctgtgc
     2341 tgccacgcta aaccagcgca gcgtcaggcc ctgcggtgga aaactaaacg ccgcatcttc
     2401 agtgttaaac gcataggcgg cgattatcag gatgggaaaa tgtaggaaaa caacgccgcc
     2461 ccaggccgcc agtttgagga aaaacggtgc gcgttcagag tgcatcgaac gctcccagac
     2521 gtttcacgaa cgccaggtac agtgcgatga gaataatcgg caccagggtg aatgccgccg
     2581 ccatcggcat attgccaatc gccccctgct gggaataaac catattgccg ataaaatatc
     2641 ctggaggacc aaccagctgc gggacgataa aatcgcccag tgtgagtgag aaggtaaaga
     2701 tagagccagc ggcaataccc gggattgcca gcggcagcac cacatagcga aaggtttgtc
     2761 gtggacgtgc gccgagatca gccgacgcct gcaacaatga cggcggcaaa cgctcaagcg
     2821 ccgcctgaac gggcaggatc atcgagtagg cagcctgacg gctgcggctt gtcatggtct
     2881 gcaattaccg ttataaaaac aggcaatgtc attgtctttc aggtggttat atggcccgtt
     2941 cagctaaacc ccgtaaacgt aaacccgcct ctcaaagttc ctgagtcgtg acccgttcga
     3001 atgcgtgctg tgtggcggcc ggatggtata ccgccgggcc attgcggggc tgaatgtgga
     3061 ggggctgaag aaaaacgcgc gggatatcag tctgctgagg tatatgccgg cctgacacag
     3121 gctaagtgtg cccgggaatc ggtgcaagta taaaaaacag cctgttgcgg tataagaatc
     3181 gaccatgcag agagagaaaa caccggagtg gagggaaaag cagaaaagct cacgtggcat
     3241 aaggcgtgga cagcggtatc gtctggtttt tcaatttcct atac
//
LOCUS       DAZOZX010000257         3280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZOZX010000257 DAZOZX010000000
VERSION     DAZOZX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.262"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..263
                     /gene="yobA"
                     /locus_tag="ACHUPT_004123"
     CDS             <1..263
                     /gene="yobA"
                     /locus_tag="ACHUPT_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0821438.1"
                     /translation="AQVTAAPQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNE
                     QDQKQLIVPLADSLKPGTYTVDWHVVSVDGHKTKGHYTFSVK"
     gene            267..1139
                     /gene="copD"
                     /locus_tag="ACHUPT_004124"
                     /pseudo
     CDS             267..1139
                     /gene="copD"
                     /locus_tag="ACHUPT_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            1152..1493
                     /locus_tag="ACHUPT_004125"
     CDS             1152..1493
                     /locus_tag="ACHUPT_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0821439.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1886..2963)
                     /locus_tag="ACHUPT_004126"
                     /pseudo
     CDS             complement(1886..2963)
                     /locus_tag="ACHUPT_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2929..3210)
                     /locus_tag="ACHUPT_004127"
     CDS             complement(2929..3210)
                     /locus_tag="ACHUPT_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0821440.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT          783 a          750 c          751 g          996 t
ORIGIN      
        1 acgcgcaagt gacagctgcc ccgcaggcaa tcactttaaa cttctcggaa ggcgttgaaa
       61 caggattcag tggtgcaaaa atcacggggc caaaaaacga aaatattaaa acattaccag
      121 cgaagcggaa tgagcaggac caaaagcaac tgattgttcc actggccgat tcgctgaaac
      181 ccggaaccta caccgttgac tggcatgttg tttcggtgga cgggcataaa acgaaaggac
      241 actacacctt tagcgtgaaa taaatcatgc tggcgtttac ctggatcgcg ctgcgattta
      301 tccatttcac ctcgttgatg ctggtctttg gctttgcaat gtatggtgcc tggctggccc
      361 ccttaatgat tcgtcgcttg ctgacgaagc gttctctgcg tttacaacag cacgctgccg
      421 tctggagttt gatcagtgcc actgcaatgc tcgccgttca gggcgggttg atgggaacgg
      481 gatggacaga tgtattttcg ccaaacattt ggcaggctgt tttgcaaacg cagtttggtg
      541 gcgtctggtt atggcaaatt gttctcgccc tcgtaacgtt gatcgtcgcc cttatgcaac
      601 cgcggaatat gccacgtttg ctgtttatgc tcactaccgc gcaatttatt ctgctggcgg
      661 gagtggggta tgcgacgctg aatgaagggg taacagcgaa aatccatcag actaatcacg
      721 cgattcacct gatttgtgcg gcggcgtggt ttgggggatt actgccggtg ctctggtgta
      781 tgcaactcat caaaggccgt tggcgacact aggctattca ggcgctgatg cgtttttcct
      841 ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg cgtgcttaat gcattgttga
      901 ttaccggatt tccccccacg cttacgactt actggggcca actcttattg ctcaaagcca
      961 tcctggtgat gattatggtg gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca
     1021 tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg
     1081 cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc gactctcgaa cctttttgat
     1141 ggattacgaa aatgatgaaa aaaagtattc tggcgtttct gttactcacc agttctgcag
     1201 cggcgctggc ggctccacag gtgataaccg tgagtcgttt tgaagtgggt aaagacaaat
     1261 gggcgtttaa tcgcgaagag gtaatgctga cttgccgacc gggtaatgct ttgtatgtca
     1321 tcaacccaag taccctcgtg cagtatcctt taaacgatat cgcacaaaag gaagttgcca
     1381 gtgggaagac caatgcccaa cccatttcgg tgattcagat tgatgatcct aacaatcccg
     1441 gcgaaaaaat gagtctggca ccgtttatag aacgagctga aaaactctgt taattaccta
     1501 aaatagcctt ttgatttcca ataaaaaaac cgcctcagtt ctttcaccag aacgggcggt
     1561 ttttaacatt taagctgatg agcaccgcgc tttttattga ccattttgca cgcaaactgg
     1621 aaaacctggc gtcgtcatct attcttaaag ggcaaggcaa ctaagcctgc attaatgcca
     1681 acttttagcg cacggctctc tcccaagagc catttccctg gaccgaatac aggaatcgta
     1741 ttcggtcttt ttttaattgt atttaaaatc aattagttgc aaacgtctcc ccgaaattcc
     1801 ccgaaattta ctcgaatttc tgtattccgg tcttttttgg ttatatcaca accaaaatac
     1861 atttaacaat ccatttacgt taaaatcaga gcagtaagta cgttttttct ctctcatcaa
     1921 gatacatttt tgttgtcttc tccgatgtgt ggccaagtag acgctgagca aattcttctc
     1981 cacatatttc tttgtacaat cgtccagcca ggcttctgat ttcgtgaaaa gttggtgggt
     2041 tttcactgaa cagtgatacc tgttaatttt ctggctgcga caaatttctt tgtcaggccg
     2101 tccgggtgaa tgctgccgtc agggctgttt tttctaatcc cggcactgat tagataatcc
     2161 cccctactta ccatgcggca ctgttcaact actgcaccaa gtcgtaaacc agcgacagga
     2221 aggctgagtg acagggggat agcaatcatc attcctgtct taatttgcct gatgtggaga
     2281 cgatcatcat aaatatcact gaaccgcata ttcgttatgt cttcgcgacg ttgtcctgtt
     2341 acaagggcta agtccatagc taatgggaac catgccggaa gttgatctgc tgcctccctg
     2401 atgcagttgt atgtctttag tttcagccgt tctcttgtaa ctactatttt cggtgctctt
     2461 gttggcgtta ctggattttg agatatacgt ccttcaacaa tggcttcgcg gaacatatca
     2521 gataacacag aacgcattga tcctgccatc gtgtttttcc ctccttcaat ctacaaatca
     2581 agaaactcgg cgatatggcg agtggttatt tctgtcagta aaacccctcc taatttttct
     2641 tttattgtct ccagttgatt tacccgagtt ttataagtat ttctggacgc ttttctcctt
     2701 ataagaatcg ttttgtaacg ttcaatccag cctgccatag taaatgagtc gaccccttta
     2761 agcttttcaa ttagggcagc aggagagtag tttttgtata tataatgatt tgcttcaatt
     2821 gcctgcgcta ctgcatctct tcttgaaatt ttacctagtg taaattcttc tttcgtcaga
     2881 gggttgcgcc agtaatatgc tttgtctctc cttcgatatg ttaagtttct aggcaaattg
     2941 ggatcgtatt ttttccgctg catgttttaa cttctccatt aacggactgt ctctcccttg
     3001 tcgcccatta ggctgatggt gtgttatatc ggtatcaacc ttatttgggt tgatatagaa
     3061 agcctccgga accaccctgt aactcctccc gtgtagttca ggtgcaggat aaatgtttcc
     3121 attccttgcc catcgtctca gcgttgatat tgatggtggg ttatccggat atctgagttt
     3181 tccccacgtt ttgagtgtca caagattcat tgccatacct ctcacgatat gaccgccagt
     3241 aaatatacag aatactggcg ggtgtggttg atttttaata
//
LOCUS       DAZOZX010000258         3179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZOZX010000258 DAZOZX010000000
VERSION     DAZOZX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.263"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPT_004128"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPT_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0821441.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHUPT_004129"
     CDS             complement(1772..1906)
                     /locus_tag="ACHUPT_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0821442.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHUPT_004130"
     CDS             complement(2552..2722)
                     /locus_tag="ACHUPT_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0821443.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZOZX010000259         3170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZOZX010000259 DAZOZX010000000
VERSION     DAZOZX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.264"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(8..634)
                     /gene="dsbA"
                     /locus_tag="ACHUPT_004131"
     CDS             complement(8..634)
                     /gene="dsbA"
                     /locus_tag="ACHUPT_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0821444.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(651..1637)
                     /gene="srkA"
                     /locus_tag="ACHUPT_004132"
     CDS             complement(651..1637)
                     /gene="srkA"
                     /locus_tag="ACHUPT_004132"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0821445.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1714..1983)
                     /locus_tag="ACHUPT_004133"
     CDS             complement(1714..1983)
                     /locus_tag="ACHUPT_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0821446.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2053..2637
                     /gene="mobA"
                     /locus_tag="ACHUPT_004134"
     CDS             2053..2637
                     /gene="mobA"
                     /locus_tag="ACHUPT_004134"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0821447.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2619..3146
                     /gene="mobB"
                     /locus_tag="ACHUPT_004135"
     CDS             2619..3146
                     /gene="mobB"
                     /locus_tag="ACHUPT_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0821448.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          771 c          762 g          805 t
ORIGIN      
        1 cattgaatta tttttcctcg gacagatatt tcaccgtatc agcatactgc tgaacaaaaa
       61 catccatatt gctggtatcc ataccctgcg gattcagctg atatttaccg ttaacaaaca
      121 tcgccggaac accacgcaat tgcacatcag ctgcggcttt ttcctgctga gcgaccagag
      181 atttcaccac gaagctgttc cacgccgcgt cgtactcttc acctttaata cctgcattga
      241 taaatacatc gcggatatca gatgctgaac gaatggtctg ggttttctgt acgccttcaa
      301 acagcggaac agtcactttg tcttccacgc ccagcgccat cgccacagcc catgcctgag
      361 tcagatcttt gcccaggtca ccacccatga agttgacgtg gtatttagtc atcttcacgc
      421 cttccggcag ttttttcttc acattatcag aaatatgcag aacttcttca aactgatagc
      481 agtgcgggca gaagaaagag aaaaactcca gcacttgcgg cgcgccagct accggttttt
      541 ccagggtagt gtactgttta ccatcttcat actgcgccgc cgatgcgcta aacgctaaaa
      601 ctaaaccagc cagcgccagc caaatctttt tcatgatcta ctctctccga ttaatacata
      661 ggtgttaatt gcaaaggggg ttcttgtaga acttttgcct gttctataaa agtcgccgtc
      721 tgtcgtagcc agtaatcttc cccggttaac cacgggaaat ttttcgggaa cgcgggatca
      781 gcccaatgcc gcattagcca ggcaagataa taaaccaaac gcatggcgcg taaaggttca
      841 atcagtccga tttcagcggt gtcgaactcg ctaaattctt cataagcttc aataatagtt
      901 tccagttgca tccgctgctg ggctttatcg ccattgagca acatccacaa atcctgaata
      961 gctggaccat tacgtgcatc atccagatca acaaacattg gaccatcgcg ccagagaata
     1021 ttcccggcgt ggcagtctcc atgtagccgc agaacggtga aatcttcccg ccagtgtgct
     1081 gtaacggcgg cgatcagctc atctgtcgct ttcagaaatg ccgctttcaa cccggaaggt
     1141 atcagtgtag cgtcctcaaa cagcttgcga ggctcaatga gatattcatt cagaccgatg
     1201 gtcgggcgat gaataaaaag ctgtttgcgc cccgtctggt gcatacgccc taaataacgc
     1261 ccaaccgctt ccatctgatc gatattatca gcttcgaact ggcgaccacc gacgcttgga
     1321 aaaacagcga aataaaatcc ctgatgattc aataaagtct gaccgttaaa ggccacaggt
     1381 gctgcgaccg gaacttcatc atttaccagc tgcaacgcaa attgatgttc ttcgaggatt
     1441 tgatcggctg tccaacgttc agggcgataa aatttgacga caaaacgtcg acgctcttcg
     1501 tcctgaaatt gatagacacg gttttcatag ctgttaagcg gggtaagacc ggaatccacc
     1561 cggatccctt gcttaaacag agcgtccatg atggtatccg ggtgtagtgt ctggaaagta
     1621 aaagcgctgt tgttcatccc atcatccgga aaacattacg aatgatgaag gatatcattt
     1681 cacgatgatt ttggtggcgc cgcttacaag cttttactct ttaattacgc cgcgagcgcg
     1741 taacagcgcc gttttgaagt cttcttcata atctttctgc aaacccggga tcaccgcatc
     1801 tttcgcggaa tcacgcattt tcaggtgata gatcagaatg tcatccgtca aatccgccag
     1861 ttcgccgtca aaacctgact ctttcgccag tttctgcaaa aattgcaaca agttaaagtc
     1921 tggctctttt tgccaggctg gctggaggag ttcaataact tcattcagac gtttacattt
     1981 catagtaatg ctccttactc ttgagacaga cacgttagca gggtcaatcc cacaataaaa
     2041 gaggcgatat cggtgaatct aatgacgacg ataacaggcg ttgtgctggc aggcggtaaa
     2101 gccagacgaa tgggcggcgt agataaagga ttgctggact taaatggcaa accgctgtgg
     2161 caacatgtcg ctgacgcgct taagacgcag ctctctcacg tcgtggttaa tgctaatcgt
     2221 catcaggaaa tctatcaggc aggcggtctg aaagtgattg aagattcact ggcggattac
     2281 ccaggccctc tggcaggaat gctttcagta atgcagcagg aagctggtga gtggtttttg
     2341 ttttgtccgt gcgatacgcc ttacattccc catgatttag ccgcccggct taatcatcag
     2401 cgcaaagatg cgcctgtcgt gtgggtccat gacggtgaac gcgatcaccc gactattgct
     2461 ctggtaaacc gcgctattga gcctttatta ctggaatatc tgcaagcagg agaacgccgg
     2521 gtaatggcat ttatgcgtct ggctggcggt catgcggttg atttcagcga tcataaagat
     2581 gcatttgtta acgtgaatac gccagaggag cttgcccgat ggcaggaaaa acgatgatac
     2641 cgttactcgc ctttgccgcg tggagtggta ccggaaaaac tacgcttttg aaaaaactga
     2701 tcccggcatt atgcgccaga gggatccgtc cggggctgat taagcatacg caccatgata
     2761 tggatgttga taagccaggc aaagatagct atgagctgcg caaggctggc gcggtacaaa
     2821 ccatcgttgc cagccagcaa cgatgggcct tgatgacgga aacaccagac gaagaagagc
     2881 tggatctaca ttttctcgca agtcgaatgg atacctcaaa gctggatttg attctggtcg
     2941 aagggtttaa gcatgaagag atcgcaaaga ttgtgctgtt tcgcgatgga gccggacatc
     3001 gacctgaaga attagtgata gacaggcatg ttattgctgt agccagtgat gtgccgctta
     3061 atcttgatgt cgcattactg gatattaatg atgttgaggg gctggctgat tttgtggtgg
     3121 agtggatgca aaagcagaac ggataagcaa gagtaggctg gatcagacgc
//
LOCUS       DAZOZX010000260         3124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZOZX010000260 DAZOZX010000000
VERSION     DAZOZX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.265"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            202..534
                     /gene="hdeA"
                     /locus_tag="ACHUPT_004136"
     CDS             202..534
                     /gene="hdeA"
                     /locus_tag="ACHUPT_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0821449.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            650..976
                     /gene="hdeB"
                     /locus_tag="ACHUPT_004137"
     CDS             650..976
                     /gene="hdeB"
                     /locus_tag="ACHUPT_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0821450.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            1040..1686
                     /locus_tag="ACHUPT_004138"
                     /pseudo
     CDS             1040..1686
                     /locus_tag="ACHUPT_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(1728..2258)
                     /locus_tag="ACHUPT_004139"
     CDS             complement(1728..2258)
                     /locus_tag="ACHUPT_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0821451.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2414..2980)
                     /gene="slp"
                     /locus_tag="ACHUPT_004140"
     CDS             complement(2414..2980)
                     /gene="slp"
                     /locus_tag="ACHUPT_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0821452.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          918 a          609 c          676 g          921 t
ORIGIN      
        1 aaacgcgtct aagaatgcag tcgatttaat aaaaatttcc taattgcagt atctgatgca
       61 tctgtaactc attgtattga aataaaaata tctgattttg atattttcca tcaacatgac
      121 atatacagaa aaccaggtta taacctcagt gtcgaaattg attcgtgacg gctctttcac
      181 tttatagttg aggatattac gatgaaaaaa gtattaggcg ttattcttgg tggtctgctt
      241 cttctgccag ttgtgagcaa tgcagcggat gcgcaaaaag cagctgataa caaaaaaccg
      301 gtcaactcat ggacctgtga agatttcctg gctgtggacg aatccttcca gccaactgca
      361 gttggttttg ctgaagcgct gaacaacaaa gataaaccag aagatgcggt tttagatgtt
      421 cagggtattg caaccgtaac cccagctatc gttcaggctt gtactcagga taaacaagcc
      481 aactttaaag ataaagttaa aggcgaatgg gacaaaatta agaaagatat gtaattccgg
      541 gaatgcgtta catcgtactt ccttgcatat tgaacaggcc ggaatatctt ctttaaaagc
      601 agctattcct cctgttcata tataatctct atattgaatg ggttacaaaa tgaatatttc
      661 atctctccgt aaagcgttta tttttatggg cgctgtagcg gctttgtcac tggtgaacgc
      721 acaatctgcg ttggcagcca atgaatctgc taaagatatg acctgccagg aatttattga
      781 tctgaatcca aaagcaatga ccccggttgc atggtggatg ctgcatgaag aaacagtata
      841 taaaggtggc gataccgtta ctttaaatga aaccgatctc actcaaattc ctaaagtgat
      901 cgaatactgt aagaaaaacc cgcagaaaaa tttgtatacc ttcaaaaatc aagcatctaa
      961 tgacttgccg aattaatgag gtacaagtaa aaaggagtag caagttgagc catcttgctg
     1021 ctcctttttg catttttata tgacagcaga atttattata cgtcttatac tcgcggcaat
     1081 tgcctgtggc gctattggca tggaaaggca aatgcgcggc aaaggagcag ggttacgcac
     1141 acatgtatta attggcatgg gaagcgccct gtttatgatt gtttcgaaat atggttttgc
     1201 tgacgtgctg tctttagatc acgtcggact cgaccccagc cgtattgctg ctcaggtggt
     1261 gacgggcgtc gggtttatcg gtgcaggtaa cattctcgtt cgtaaccaaa atattgtagg
     1321 tctgacgacg gcagcggata tctgggtgac cgccgcatag gtatggttat tggcagcggt
     1381 atgtacgaac tgggtattta tggttcagtg atgaccttgt tggtgctgga agtcttccat
     1441 caattaacct tccgcctgat gaacaaaaat taccatttac agctgacatt agtgaacggt
     1501 aatacggtat caatgctcga ctggtttaag cagcaaaaaa taaaaactga tttagtttcg
     1561 ttgcaggaaa atgaagatca tgaagtggtc gccattgata tccagcttca tgccacaacg
     1621 ttgatagaag acctgttgcg attattaaaa ggtatggcag gggtgaaagg cgtttcaatt
     1681 agctaatctg tgtggcagcg tagccagact caccgtaagc ctgaaattca caccagataa
     1741 tcaatatgct gatggcgtac cagctcattg atccgcttaa gattgaggat catcatgata
     1801 ttgtgtttat ggcagtagaa cgtttttagc gatattttca gtttctccag aatatcttca
     1861 gttgtcattt gattcgcaat gcagctcaga acctgatgtt gcttgcgcga aagtgacaca
     1921 ttcgcttcag gcacagagca gtaactgcca ttgatgagtg cgacaaaatc aatgatagtg
     1981 atagtccgcg gaacaaaggt caccggtact ttccccagac agcgtttaat attaaagggg
     2041 gagaaaacaa tgacatggac aggttttaat cgttcaattt gagtgagaaa attagaatga
     2101 aatacattat tcattaatgt atcaataatg acgaaggcat tctgatgcaa cattacgtcg
     2161 atctcttctt cgttctgtat atgaacgaca ttacctttac tcagaatgtt tttcatcgcg
     2221 gtgaagaaca tcgtatccct ggtaattata agaaacataa ccgactcctg gtgcggacat
     2281 taactttata atcagtttgc caaaggtaat taacaggaca gggcttataa taataatagt
     2341 tagcttactg tatattaatt ttatgtaata catgatgcag atgctgagaa agcatctgca
     2401 tctttcggtg gtgttatttg accagctcag gtgttacctg actcaccgca ttggtgtagt
     2461 aaggcgcacc ccagcccggt tccggccaga atgcaccata gccgtaatcc cacaggttat
     2521 aggtggtatt aaccacttct ctcaaatgcc acacctggat gccctgcata ttcacttcca
     2581 ggaagttata cgggacttta ttgataaagc ctggttgttc accctgaatg gtaccgagga
     2641 tggtaacaaa gtgattacga tagttcactg gatcaaggaa gccgctttgt ctggcgagca
     2701 gtcggccctg atagttggct tcaatatcag gcttcgcata gctatccaac ggtaatacag
     2761 cgatttctaa caacgtatcc gttttgccat tgataacgtt gataaccttc ccaccaaagc
     2821 gcgcttgttg accaacatat aaccccggct ggttatgaac agcaacaaaa cttttttgaa
     2881 tatcaggttg gttattgcct ttgatatttt gcggaattga actacatgcg gcaagcaaaa
     2941 atgaaaggct gaggatgagt gcaccttttg tcatgttcat gttactatcc ttatcaacaa
     3001 atattaaaac cataatagat atttctttat agttttcatc tgattctgag gtgatatgcc
     3061 tgatagtaac gtaaacctgt ctgatgttta tatcccttct gtttagttca attccgttat
     3121 tttg
//
LOCUS       DAZOZX010000261         3106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZOZX010000261 DAZOZX010000000
VERSION     DAZOZX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.266"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(71..190)
                     /locus_tag="ACHUPT_004141"
                     /pseudo
     CDS             complement(71..190)
                     /locus_tag="ACHUPT_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            434..806
                     /locus_tag="ACHUPT_004142"
                     /pseudo
     CDS             434..806
                     /locus_tag="ACHUPT_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1368..2315)
                     /gene="icsP"
                     /locus_tag="ACHUPT_004143"
     CDS             complement(1368..2315)
                     /gene="icsP"
                     /locus_tag="ACHUPT_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR0821453.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          914 a          671 c          603 g          918 t
ORIGIN      
        1 caactacaaa gttaattgat cctacctaca taatatagac acagtaccta aagtaccgtg
       61 gagatcatcc ggggaagccc cgggtctgat accagtgcag gtagaccgct atcttgttcc
      121 ggtaatgttc tgccgttctt tacggcatca cctctagcag aaacatcgcg taactgcgcc
      181 aagtatgatg aacttctctt accgctgcga tctggttcaa caatatgcag atggtacaca
      241 acctctttaa ccagcccatc cggattattc agagccgctt ccgcaatccg gaataaaagt
      301 gctgttttac cgtgaaccct ctgaaaatca gcgagcagtg ttttaaccag ccgacgttca
      361 gagcgtactg acagacggtg gacaatttga ataaacagtt caatcagacc gtagatgctc
      421 tcccgttggc gacatgctgg gagcggtgga acaccgcttc ggcaacgagc ttccggcgtc
      481 tccagtgaag tgactgacgg ataatggttc atgctaccga gctaatgaaa cacgccagtt
      541 cacccggatg ttgggacttt gttgaaccga agaacacggc ggtgcggagt ccggagagta
      601 acagaatagc aaagagcttc gtgaaaacaa taaagtgtga ctacatcagt atcatgccca
      661 aacctgacgg gttaacggca gcaaagaacc ttgcagaggc attcgagcat tataacgaat
      721 ggcatccgca tagtgcgctg gattatcgct cgccacggga atatctgcgg cagcgggcca
      781 atgataacag gtgtctggaa atataggggc aaagccaccc acgaacgatc cagcatataa
      841 tcaagaaatt agatttcatt taacataaga tagattatgt acaccaagag gcgtttcctt
      901 aatttctgaa tttacaggct cttttgctct ctttgctatg ctgtggattt ggaaagatag
      961 agtgctggct gattatttag gtattttcat cagcatgtcc ctgatagcac tgttccatca
     1021 gcgctttcag gcgataatag tagaccttgg gtagcctctg cccatgtgta acttgatacc
     1081 ccgagaacag ccacgatgat ctaagcggtt cttatttctc cggtctcagg gtcggtaatg
     1141 ccaacagtaa gtccacagta atcaacgaag agttttttga accgccccgg aaatcctgga
     1201 gactaaacgc cctgagaaag aggtaaacag aatgactaaa aatacacgtt tttcccccga
     1261 agtccgtcaa cgggcagttc gtatggttct ggaaagtcag ggcgaatatg actcacaatg
     1321 ggcggcaatt tttagccatg aaaacgtaat caactcggtg aaccaattca aaaaatatac
     1381 tttatacctg cggaagtgag aaaaccaata tattcaatac ttgcactatt tttaatggtg
     1441 ccagatatat tggtactttg ctcataaaga gatgtatcac cttttttatt agaaatgtaa
     1501 tttctggagc cttctatata aaattttgca ctgggggtga catagtatcc aatattcgca
     1561 gcaacagagc agtaattttg gttttttatt tcatctttaa aaattgtctc agtctgatag
     1621 tgtttatctg tatcagatga aagaacccaa ccactatatt tcagttctgc tccaaactca
     1681 aaattctcat ggcggtaatt agcagttaat ccaatataag gtattttgaa gagctgtttg
     1741 taacctattg ttctttcacc actaggatgt gccccttttt tattcctgct ccccccgttt
     1801 tcactataaa tataactccc tcccattgca ttaaaactgt aacgactctc ttggtaacct
     1861 gctattagtc caagtcgata atccaaatta tttaataacc aacctttaag attcagctcg
     1921 aattcgttag catcacggag atgcgtattg ggatgccagc tctggtcggt ccatacctga
     1981 ggggtattgg aattgttcca gtctttatca accatgcttg ctttctggtt acctaaagta
     2041 gtccaaccgg aaactccgaa agacactttt ggaattagct tccaatcgat gccacctcta
     2101 acaatagtcg cattactgta tttccagtcc agttgactaa tttttctccc cccttccttg
     2161 ggatgataaa cgcgttcttt tgttttgcca ctcagagatc ctagactaat atccgtactg
     2221 atgttgtccg gtatgaaaag tggatagtta gtggtagcat gtgtagtaaa gatcgcaggt
     2281 acacaaagtg caagtacaaa gaattttaat ttcatcgaga actctacttt tttggttgaa
     2341 atgtccatga tcttcttact ttataagata aacgtcaatg ccaagcatta ttttaattaa
     2401 atatttgttt atgttaccaa gaacatatct tttattataa tatttattga acttttgtaa
     2461 aaataaaaaa atacctcatt gtgcgaataa agtaacgggg gccatagatt tctctaccta
     2521 ccttttggtg ttcatacctg aggctgatag tttcaggatt ccgcgacaat tctactcacc
     2581 ttcttgttct ctcgccaata tgaaatcaat cagaaaagcg ttccataaga acaacgataa
     2641 gacaataaat tgtcatcacg gctgcaatag ccaacgtgcc tttcagtacc aacatgacaa
     2701 cctcctgtat ttgacgtaac gtgtgctgat aaatatgtat ttgtatctca actgactcaa
     2761 tacaacactt aagtatagtt tttcagattt tgtttttccc gtgaaatatt atggaaatgg
     2821 cagaagcaat acattgaatt ccgattcgag tgaatacggc tagcgggcac ctcactttag
     2881 cactgaagcc atcaactttt atttgactta tttcaatgga aagctttctc actatcagta
     2941 caggtaattc ccatcctttt aaagtcgaga gtcagactgc gactaataca gttaaagtga
     3001 ccaaactcag ttatcctcta cacattttat ctcatgttta cttggacaat tcattggtgc
     3061 ttttataaaa gaataaatat tctactaatt taatgcatca cctaaa
//
LOCUS       DAZOZX010000262         3070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZOZX010000262 DAZOZX010000000
VERSION     DAZOZX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.267"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..725)
                     /locus_tag="ACHUPT_004144"
     CDS             complement(<1..725)
                     /locus_tag="ACHUPT_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR0821454.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLA"
     gene            918..1421
                     /locus_tag="ACHUPT_004145"
     CDS             918..1421
                     /locus_tag="ACHUPT_004145"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0821455.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1467..1883)
                     /gene="rraB"
                     /locus_tag="ACHUPT_004146"
     CDS             complement(1467..1883)
                     /gene="rraB"
                     /locus_tag="ACHUPT_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0821456.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            2045..3058
                     /gene="argF"
                     /locus_tag="ACHUPT_004147"
     CDS             2045..3058
                     /gene="argF"
                     /locus_tag="ACHUPT_004147"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0821457.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          918 a          707 c          672 g          773 t
ORIGIN      
        1 gcgagaaaag gtaataaagc taaaaatgcg aggatggcat attttataca atacgcagta
       61 tctaatttca ccttgaaacg atgtataccg aaactggtat tgctccagag aaaactcatt
      121 accaggctat ataaagtacc attaaaaata ccaatggaaa caataccaac tattgccatc
      181 agaaccacag atataataac actacttgaa ctattggcat gtagcatctt tgtcgagata
      241 aagaaaacag tccccatccc aatggccatt aaaatcggca agaaaaaggt tacccaccag
      301 aaccctttca aagagcaatt aaaactaaat cttacgccgt tgagactggt cattaaggcc
      361 tgataacgca gtccttttgc tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa
      421 acacagccaa caagcggcat atctgctgac actgtcatta agattgcgaa atagaaaaaa
      481 acaaaaacaa gacaactaac aaaaacattc ccaccggtaa ttccataaga aaatcgttgt
      541 ccgttaactt ccatattagc ataaagataa cgcttacatt tcattaatgc ccatggtaaa
      601 tagataccta gcgttataat cgttaacaac acattcacca cacaaataag aaaatatctc
      661 tcacctgtac catgaaaaac aaaagaatgg gacggaacat ccatttcatt aataacttga
      721 gccataaaaa tcccctgaat taaaaaatcc attgatataa tttatagata cagacagtaa
      781 aatgttctgc cacgttacaa tactcttatc agtaataggc tggcaaaaac tttattgctt
      841 tataagacgc ttgcgcacgt aagaagagag tggtattaaa caaatgtata attactttat
      901 ttttattggg ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga
      961 cgctgcagga taatcctgct atcgccagcg tcatccgtca ggtttacgca gagtatggtc
     1021 ttaccgccga taaaggctac accgttgccg atccgaatct cgacgaactg tatcaagtgt
     1081 acagccagcc tggccatgct tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg
     1141 gcgggattgc gccattaaca ggtagtgaag cggacatttg cgaactgcag aaaatgtact
     1201 ttcttcccgc tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc
     1261 aggcacgaga gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag
     1321 ccattgcgct ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg
     1381 gccatgtcga ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg
     1441 tgctatcggg cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca
     1501 aaatcttcat cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg
     1561 tactcaacgt caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga
     1621 tcggcattca acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca
     1681 acttccagct cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct
     1741 ttttccagag tttccagatc gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc
     1801 ggatcgctgc catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct
     1861 tccagttgtt ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt
     1921 gtcacacacc actgacattg cctccacctt tgtgacaaag atttatgctt tagacttgca
     1981 aatgaataat aatacacata aattgagttt taattcattg aggcgttagc cacaggaggg
     2041 atctatgtcc gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga
     2101 actcaacagc ctgctgcagt tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga
     2161 agaagccaaa ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgactcgtac
     2221 ccgatgctct ttcgaagttg ccgcatatga ccagggggct cgcgttactt atcttggccc
     2281 aagcggtagt cagattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg
     2341 catgtatgac ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga
     2401 atacgctggc gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagttgct
     2461 ggcggatctc ctcaccatgc aggagcattt acccggtaaa gcattcaacg aaatgacgct
     2521 ggtctatgca ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcggcgct
     2581 taccggtctg gatttgcgtc tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt
     2641 tacggaatgc cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt
     2701 agctaaagga gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga
     2761 agcaaaagaa aaatgggctg aacgcattgc attactgcgt gattatcagg tgaacagcaa
     2821 gatgatgcag ttgaccggta acccggaggt caaattcctc cattgcctgc ccgcgtttca
     2881 tgacgaccaa acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat
     2941 ggaagtgact gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa
     3001 ccgcatgcat accatcaaag cggtgatggt cgcgacgctc agtaaattaa ataattaata
     3061 tctctataaa
//
LOCUS       DAZOZX010000263         3069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZOZX010000263 DAZOZX010000000
VERSION     DAZOZX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.268"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHUPT_004148"
     CDS             <1..498
                     /locus_tag="ACHUPT_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0821458.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHUPT_004149"
     CDS             578..1273
                     /locus_tag="ACHUPT_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0821459.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHUPT_004150"
     CDS             1302..>3069
                     /locus_tag="ACHUPT_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0821460.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZOZX010000264         3026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZOZX010000264 DAZOZX010000000
VERSION     DAZOZX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.269"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..2850
                     /locus_tag="ACHUPT_004151"
     rRNA            <1..2850
                     /locus_tag="ACHUPT_004151"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2946..>3026
                     /gene="rrf"
                     /locus_tag="ACHUPT_004152"
     rRNA            2946..>3026
                     /gene="rrf"
                     /locus_tag="ACHUPT_004152"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          799 a          667 c          943 g          617 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg acccgtgatc
     2161 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
     2221 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
     2281 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
     2341 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
     2401 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
     2461 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     2521 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     2581 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     2641 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     2701 ctaagcacga aacttgcccc gagatgagtt ctccctgacc ctttaagggt cctgaaggaa
     2761 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     2821 cggtactaat gaaccgtgag gcttaacctt acaacgccga agatgttttg gcggattgag
     2881 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2941 attagcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     3001 aacgccgtag cgccgatggt agtgtg
//
LOCUS       DAZOZX010000265         3014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZOZX010000265 DAZOZX010000000
VERSION     DAZOZX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.270"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            32..625
                     /gene="tetC"
                     /locus_tag="ACHUPT_004153"
     CDS             32..625
                     /gene="tetC"
                     /locus_tag="ACHUPT_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR0821461.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(738..1943)
                     /gene="tet(B)"
                     /locus_tag="ACHUPT_004154"
     CDS             complement(738..1943)
                     /gene="tet(B)"
                     /locus_tag="ACHUPT_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR0821462.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2025..2648
                     /gene="tetR(B)"
                     /locus_tag="ACHUPT_004155"
     CDS             2025..2648
                     /gene="tetR(B)"
                     /locus_tag="ACHUPT_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR0821463.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2626..>3014)
                     /locus_tag="ACHUPT_004156"
     CDS             complement(2626..>3014)
                     /locus_tag="ACHUPT_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0821464.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
BASE COUNT         1029 a          619 c          570 g          796 t
ORIGIN      
        1 catactgacg gtatgttatt ttaaatctat catggaaaat aaaaatcatc aacaagaaaa
       61 ttttaagagt acctatcaat cactggttaa ctcagcacga atattgtttg ttgaaaaagg
      121 ctatcaagct gtttcaatag atgagatctc gggaaaagcg ttggtgacca aaggtgcctt
      181 ttatcatcac tttaaaaata aaaaacaatt actcagtgcc tgttataagc agcaattaat
      241 tatgattgat gcctacatca caacaaaaac tgatttaaca aatggttggt ctgccttaga
      301 aagtatattt gaacattatc ttgattatat tattgataat aataaaaacc ttatccctat
      361 ccaagaagtg atgcctatca ttggttggaa tgaacttgaa aaaattagcc ttgaatacat
      421 tactggtaag gtaaacgcca ttgtcagcaa attgatccaa gagaaccaac ttaaagctta
      481 tgatgatgat gtgcttaaaa acttactcaa tggctggttt atgcatatcg caatacatgc
      541 gaaaaaccta aaagagcttg ccgataaaaa aggccaattt attgctattt accgcggctt
      601 tttattgagc ttgaaagata aataaaatag ataggtttta tttgaagcta aatcttcttt
      661 atcgtaaaaa atgccctctt gggttatcaa gagggtcatt atatttcgcg gaataacatc
      721 atttggtgac gaaataacta agcacttgtc tcctgtttac tcccctgagc ttgaggggtt
      781 aacatgaagg tcatcgatag caggataata atacagtaaa acgctaaacc aataatccaa
      841 atccagccat cccaaattgg tagtgaatga ttataaataa cagcaaacag taatgggcca
      901 ataacaccgg ttgcattggt aaggctcacc aataatccct gtaaagcacc ttgctgatga
      961 ctctttgttt ggatagacat cactccctgt aatgcaggta aagcgatccc accaccagcc
     1021 aataaaatta aaacagggaa aactaaccaa ccttcagata taaacgctaa aaaggcaaat
     1081 gcactactat ctgcaataaa tccgagcagt actgccgttt tttcgcccca tttagtggct
     1141 attcttcctg ccacaaaggc ttggaatact gagtgtaaaa gaccaagacc cgctaatgaa
     1201 aagccaacca tcatgctatt ccatccaaaa cgattttcgg taaatagcac ccacaccgtt
     1261 gcgggaattt ggcctatcaa ttgcgctgaa aaataaataa tcaacaaaat gggcatcgtt
     1321 ttaaataaag tgatgtatac cgaattcgat tgcgtctcaa cccctacttc ggtatctgta
     1381 ttatcacgtg tatttttggt ttcacggaac caaaacataa ccacaaggaa agcgacaata
     1441 tttagcaacg cagcgataaa aaagggacta tgcggtgaaa tctctcctgc aaaaccacca
     1501 ataataggcc ccgctattaa accaagccca aaacttgccc ctaaccaacc gaaccacttc
     1561 acgcgttgag aagctgaggt ggtatcggca atgaccgatg ccgcgacagc cccagtagct
     1621 cctgtgatcc ctgaaagcaa acggcctaaa tacagcatcc aaagcgcact tgaaaaagcc
     1681 agcaataagt aatccagcga tgcgcctatt aatgacaaca acagcactgg gcgccgacca
     1741 aatcggtcag acatttttcc aagccaagga gcaaagataa cctgcattaa cgcataaagt
     1801 gcaagcaata cgccaaagtg gttagcgata tcttccgaag caataaattc acgtaataac
     1861 gttggcaaga ctggcatgat aaggccaatc cccatggcat cgagtaacgt aattaccaat
     1921 gcgatctttg tcgaactatt catttcactt ttctctatca ctgataggga gtggtaaaat
     1981 aactctatca atgatagagt gtcaacaaaa attaggaatt aatgatgtct agattagata
     2041 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     2101 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     2161 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     2221 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     2281 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     2341 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     2401 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     2461 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     2521 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     2581 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     2641 ggtcttaaaa gcagcataac ctttttccgt gatggtaact tcacggtaac caagatgtcg
     2701 agttaaccac cctttagatt cataaagcga aaataatgcg gctccaacgt acccacctaa
     2761 atggaaacgg cgttcactcc aatctaaaca cgcacaacag attttacgtg aatgtttgga
     2821 aggaacgtca attcccattt catgaaaata ttgaatacca cttaatgtga tcattgaacc
     2881 attttcagtg atccattgct gttgacaaag ggaatcatag atcttaacgg caacttcgcc
     2941 agctaaatga tcatagcaag tacgtgcttt tcgtaaatgc actggcgtgg aaactttggc
     3001 atgtacgcca tggt
//
LOCUS       DAZOZX010000266         2999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZOZX010000266 DAZOZX010000000
VERSION     DAZOZX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.271"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUPT_004157"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUPT_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0821465.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHUPT_004158"
     CDS             348..641
                     /locus_tag="ACHUPT_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0821466.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHUPT_004159"
     CDS             713..1372
                     /locus_tag="ACHUPT_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0821467.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHUPT_004160"
     CDS             1464..1910
                     /locus_tag="ACHUPT_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0821468.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..2999)
                     /gene="hlyE"
                     /locus_tag="ACHUPT_004161"
                     /pseudo
     CDS             complement(2117..2999)
                     /gene="hlyE"
                     /locus_tag="ACHUPT_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          782 a          647 c          718 g          852 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatc
//
LOCUS       DAZOZX010000267         2951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZOZX010000267 DAZOZX010000000
VERSION     DAZOZX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.272"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHUPT_004162"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHUPT_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0821469.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHUPT_004163"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHUPT_004163"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0821470.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHUPT_004164"
     CDS             2603..>2951
                     /locus_tag="ACHUPT_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0821471.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZOZX010000268         2932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZOZX010000268 DAZOZX010000000
VERSION     DAZOZX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.273"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHUPT_004165"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHUPT_004165"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0821472.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUPT_004166"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUPT_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0821473.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUPT_004167"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUPT_004167"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR0821474.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHUPT_004168"
     CDS             2756..>2932
                     /locus_tag="ACHUPT_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821475.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZOZX010000269         2898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZOZX010000269 DAZOZX010000000
VERSION     DAZOZX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.274"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUPT_004169"
     CDS             complement(<1..312)
                     /locus_tag="ACHUPT_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0821476.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHUPT_004170"
                     /gene_synonym="yehT"
     CDS             complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHUPT_004170"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0821477.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHUPT_004171"
     CDS             complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHUPT_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0821478.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          750 a          774 c          658 g          716 t
ORIGIN      
        1 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
       61 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
      121 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
      181 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag
      241 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
      301 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
      361 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      421 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      481 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      541 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      601 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      661 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      721 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      781 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      841 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      901 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      961 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     1021 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     1081 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     1141 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     1201 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     1261 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1321 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1381 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1441 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1501 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1561 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1621 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1681 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1741 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1801 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1861 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1921 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1981 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     2041 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     2101 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     2161 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     2221 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     2281 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2341 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2401 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2461 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2521 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     2581 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     2641 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     2701 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     2761 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     2821 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     2881 aataaaatca ccctatag
//
LOCUS       DAZOZX010000270         2891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZOZX010000270 DAZOZX010000000
VERSION     DAZOZX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.275"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="ACHUPT_004172"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="ACHUPT_004172"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR0821479.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="ACHUPT_004173"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="ACHUPT_004173"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR0821480.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="ACHUPT_004174"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="ACHUPT_004174"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR0821481.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="ACHUPT_004175"
     CDS             complement(1897..2811)
                     /locus_tag="ACHUPT_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR0821482.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          773 g          691 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc t
//
LOCUS       DAZOZX010000271         2862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZOZX010000271 DAZOZX010000000
VERSION     DAZOZX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.276"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUPT_004176"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUPT_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0821483.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUPT_004177"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUPT_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0821484.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUPT_004178"
     CDS             complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUPT_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR0821485.1"
                     /translation="SANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAAS
                     RITSLFLGLKIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFG
                     FHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGG
                     NIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGR
                     FKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFT
                     VWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          833 a          745 c          727 g          557 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag ag
//
LOCUS       DAZOZX010000272         2835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZOZX010000272 DAZOZX010000000
VERSION     DAZOZX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.277"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..593)
                     /locus_tag="ACHUPT_004179"
     CDS             complement(<1..593)
                     /locus_tag="ACHUPT_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0821486.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUPT_004180"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUPT_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR0821487.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHUPT_004181"
     CDS             complement(1422..1961)
                     /locus_tag="ACHUPT_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0821488.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUPT_004182"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUPT_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR0821489.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          735 a          705 c          622 g          773 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagat
//
LOCUS       DAZOZX010000273         2826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZOZX010000273 DAZOZX010000000
VERSION     DAZOZX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.278"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUPT_004183"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUPT_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0821490.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHUPT_004184"
     tRNA            complement(621..695)
                     /locus_tag="ACHUPT_004184"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHUPT_004185"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHUPT_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUPT_004186"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUPT_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0821491.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          676 a          690 c          682 g          778 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggaga
//
LOCUS       DAZOZX010000274         2807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZOZX010000274 DAZOZX010000000
VERSION     DAZOZX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.279"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUPT_004187"
     tRNA            6..81
                     /locus_tag="ACHUPT_004187"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUPT_004188"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUPT_004188"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUPT_004189"
     CDS             complement(299..1057)
                     /locus_tag="ACHUPT_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0821492.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUPT_004190"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUPT_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0821493.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACHUPT_004191"
     CDS             complement(2178..>2807)
                     /locus_tag="ACHUPT_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR0821494.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZOZX010000275         2756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZOZX010000275 DAZOZX010000000
VERSION     DAZOZX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.280"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..651
                     /gene="mxiA"
                     /locus_tag="ACHUPT_004192"
                     /gene_synonym="virH"
     CDS             <1..651
                     /gene="mxiA"
                     /locus_tag="ACHUPT_004192"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR0821495.1"
                     /translation="IDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLDQFENRY
                     PDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVITLVEHVR
                     ASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDEVMETLA
                     HALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINVLKTI"
     gene            664..1065
                     /gene="spa15"
                     /locus_tag="ACHUPT_004193"
                     /gene_synonym="spaK"
     CDS             664..1065
                     /gene="spa15"
                     /locus_tag="ACHUPT_004193"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR0821496.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            1069..2361
                     /gene="spaL"
                     /locus_tag="ACHUPT_004194"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             1069..2361
                     /gene="spaL"
                     /locus_tag="ACHUPT_004194"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR0821497.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            2454..>2756
                     /gene="spa13"
                     /locus_tag="ACHUPT_004195"
                     /gene_synonym="spaM"
     CDS             2454..>2756
                     /gene="spa13"
                     /locus_tag="ACHUPT_004195"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR0821498.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDE"
BASE COUNT          890 a          394 c          590 g          882 t
ORIGIN      
        1 attgatgcta aaattaaaag tgctcaagat gaattttatc accagttgtc acaagcttta
       61 ttaaacaaca taaatgagat ttttggtata caagaaacaa aaaatatgtt agatcagttt
      121 gaaaatcggt atcctgatct attaaaggaa gtcttccgac atgtgactat acagagaatt
      181 tctgaggtat tacaaagatt gcttggagaa aatatttctg ttcgcaattt aaaacttatt
      241 atggagtctt tggcgctttg ggctcccaga gaaaaagatg tcataacatt agttgaacat
      301 gtccgtgcat cactttctag gtatatttgt agtaaaatag ctgtttctgg tgagattaaa
      361 gttgtgatgc tttccggata tattgaggat gcaataagaa aagggataag gcaaacctct
      421 ggtggctctt tcttgaatat ggatatagag gtttcggatg aggtaatgga aactttagca
      481 catgctttga gagaattgag aaatgcaaaa aaaaatttcg ttcttttggt atcagtagat
      541 atacgtaggt ttgttaaaag acttatagat aacagattta agagtatact cgttatatcg
      601 tatgctgaga ttgatgaagc atataccatt aatgtattaa agactattta gtgaggttta
      661 aatatgagta acattaattt agttcaatta gttagagata gtcttttcac gattggttgt
      721 cctccatcaa ttattacaga tttagacagc cattcagcga ttaccatttc tttggatagt
      781 atgcctgcta ttaatatagc tcttgtcaat gagcaggtta tgttatgggc taattttgat
      841 gcaccaagtg atgtaaaact tcagtcatct gcttataaca tattaaattt aatgttaatg
      901 aactttagtt atagcattaa tgagctggta gagcttcatc gttctgatga gtacttacag
      961 ttaagagttg ttattaaaga tgactacgtt cacgatggga ttgtttttgc agagattctg
     1021 catgagtttt atcagaggat ggaaatctta aatggggtct tataatcaat gagctataca
     1081 aaattgctca ctcaattatc ttttcctaat agaatctcgg ggccaatctt ggaaacaagt
     1141 cttagcgatg tttcgattgg tgagatttgt aacattcagg ctggaattga aagtaatgaa
     1201 attgttgcaa gagctcaggt tgtaggattt catgatgaaa aaacaatatt aagcttgatt
     1261 ggaaattctc gtggactttc acggcaaacg ttgattaagc ccactgccca gtttcttcat
     1321 acgcaagtcg gccgtggatt attgggagca gtagtcaatc ctttagggga ggttactgat
     1381 aagtttgctg ttacagataa cagtgaaatt ctttatcgac ctgtagataa tgctcctccg
     1441 ctatatagtg aaagggctgc aattgagaag ccttttttaa caggtattaa ggttattgat
     1501 tctttactca cgtgtggtga aggacagcga atggggattt ttgcgtcagc tggttgtggc
     1561 aaaacttttc tcatgaatat gctcattgaa catagtggtg ctgatatata tgttattggg
     1621 ttaattggtg agcgaggtcg agaggttact gaaacggttg attatttgaa aaactctgag
     1681 aaaaaaagca ggtgtgtttt agtatatgca acttcggatt actcttcggt tgatcgttgt
     1741 aatgctgcat atatagccac tgctatagcc gaatttttta ggactgaagg acataaagta
     1801 gcgcttttta ttgattcatt aacaaggtat gccagagcat tacgtgatgt ggccttagcc
     1861 gctggagaat cacctgccag aagaggctat ccggtttcgg tttttgatag cttacccaga
     1921 cttcttgaaa ggccaggaaa gttaaaggca ggtggctcta ttactgcatt ttacactgtt
     1981 cttttggagg atgatgattt tgctgatcca ttagctgaag aggtaagatc cattttagat
     2041 ggacatatat atttgagcag aaatctagcc caaaaaggac aatttcctgc aattgattcc
     2101 ttaaaaagta taagcagggt atttacacag gttgttgatg aaaaacatcg tattatggcc
     2161 gctgcatttc gggagctact ttctgaaata gaagagctaa ggacaattat tgactttggt
     2221 gaatacaaac cgggggagaa tgcctctcag gataaaatct acaacaaaat atctgttgtt
     2281 gagagttttc tgaagcaaga ttatcgactc ggttttactt atgagcagac aatggagctt
     2341 attggtgaaa caattagata aggttttaaa aatcaaagat aaatatcaac ggtctgttaa
     2401 attaattgaa gcacacatat taacactaaa aaaaaaactc tttgtatcga gatgtggagg
     2461 cattagataa aaggattata tacttcctgc aactggagaa tgacttagaa cctgtaggcg
     2521 ctcagagtgt tagtcagctt ttcaatacaa gacgtaagat cgctatagta aaaaagcata
     2581 ttatccaata tcagtctgaa cgaattcttc taaagggaag aattgaagaa attcaaaaag
     2641 atattgatga ggcaaatgct tctaaaagaa aactgcttca taaagagagt aaaatatgta
     2701 aacggattgg cttgattaaa agaaataact ttgccaaaca gttgatactt gatgag
//
LOCUS       DAZOZX010000276         2737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZOZX010000276 DAZOZX010000000
VERSION     DAZOZX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.281"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(358..1137)
                     /gene="ipaJ"
                     /locus_tag="ACHUPT_004196"
     CDS             complement(358..1137)
                     /gene="ipaJ"
                     /locus_tag="ACHUPT_004196"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR0821499.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1334..2143)
                     /locus_tag="ACHUPT_004197"
                     /pseudo
     CDS             complement(1334..2143)
                     /locus_tag="ACHUPT_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2490..>2737
                     /locus_tag="ACHUPT_004198"
     CDS             2490..>2737
                     /locus_tag="ACHUPT_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821500.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          818 a          688 c          442 g          789 t
ORIGIN      
        1 acttataata tccatacccc tcatagggct tacagtttaa attgcgggta taaaatacat
       61 ctattttcaa ataaaataaa gcatactcca attacaacga gtgactaaaa taaaaatatc
      121 atccaagtca atcactgttt gctctgcaaa atatgtcagc cttctcaaac ttaccagcac
      181 ttaaatattt aagcacacaa tcattccttg agggagttca cttatttcaa gagaaacatt
      241 tcgtcttccc acacaactaa tccgcccaat atagctaaat ttgtactcag taatatcatt
      301 tgttatgccc ccccacccca aaatattcaa gctccttcac agataaaaaa cgacaattta
      361 caaagcctca ttagttataa ctatggaaat acctatttta gtatatccta taaagttaga
      421 attacttctt cttgcaccta attccatagt tgaaaaacaa gaataatttt ctcctacagc
      481 tggatccata tagcttccat caggtctaca caacacccag tggaggccga caggaactcc
      541 aataaaggat acagcaacag cttccaaaac tctttggtta gaagataaca catctcgttg
      601 atgtactata ttacatccca tgccaattaa taagtccctt gcatcgggat aaataaaact
      661 aattacttgc gaaaaaatgt tactttcttc aacgacataa gcattcaatc caagaagcct
      721 cgtagccatg actatcttat caggcataga ataaccggca tccgcgatat tatctttgtg
      781 tattctcgga ttataattac cactagttat taagtataaa tcccgctcac aacgattatc
      841 caaatcaaga gaactcttcc ttgtcatttc ggacatagac cctttgtatt gtggtatttt
      901 atcaacccct aattcctttg cagcacagag taaactggca gctccacaag aattgtcata
      961 catctgcttc ctcgccaaaa aacaaccttg ttcgtttact ctattctcat ttaccctcac
     1021 atcagtctga tgagatatca tatattctct tggtatagcc ccctgcaata aaaccccata
     1081 taacataaca cctgtatgaa tacatccccg tttgcaaggc ttccgttgtt ccgacatcga
     1141 cgagtaattg cagcacagta ttgcatccag caatgcgaac tactatgaga attagctgac
     1201 cgggtcctta ttccccgaac gaactttaaa tccaaaacct catactcttt tccgtcgtca
     1261 tcacgtccgt caccataaca acctttcagc atccaccctt ttattaagag ttaatgtcca
     1321 tcagcatcag caattaatac cgcctgatac tgacatattt ttgtcgtatt tacgtccata
     1381 atcggttatt gtgatacagg tcacaccagc ttatttgcta ttgttacaat aaccaccttg
     1441 gttgagagct gttttttcaa ctttgttatc aactccccca actccttggt cagtaaggct
     1501 gccgaccgag cacagtgaat agatagtatc cgcaggtacc accatgctta cttaatactc
     1561 agaaggcgaa gtgctctcca attaactctg ccgcactatg cttataagct gcttttcaat
     1621 gccgtctatc aacatgtcca attcattcaa tctgttgtac tggtaatcca acgcagtaat
     1681 gatatatgca ggtattcttt catccactcc agtaacgttt catgcaaagc attaatttac
     1741 gcgctacgga acttcaccag ttgactgagc aaacgatgtg ggaccagtac tgcctgcagt
     1801 tcttctattg ttacagcgat ttctttacca aacagctgaa ccaccatcaa gataaagata
     1861 gcctgaacat tcatcgcacc attttgttac caatggcaaa agatttaacg aaccgtgcct
     1921 gcatcaggcg gactttttat ccctgctttg ttagctccct aaccttgtac agagagcctc
     1981 cataagctcc catatcaagc agacatggtt cgcagttact gacatacacc agaaaactct
     2041 gacagcagac tcacttatcc tccacttcac tcatatcctt attggtgaga tgaacctgaa
     2101 tgacatgttt tgcgacataa acgccaacca gtgtatattt cacctgtaga gattctacaa
     2161 tctgtagcgt tatgcatcta atgttaggct ctatgatgtc agaaatctgc gagactctac
     2221 accttctgca tttaccttta tttatcacca tgctcctcag ttaacggaga acgtgttcat
     2281 tgcattttgt cacttacatt tcgcctttgg tatcgggggg atataggttc caacgttccg
     2341 catgagaacc tgaaccggct tcacgccacc ttgataccag atgccgcaca ggtagtcaac
     2401 aagtgtcacg aacggtgcaa tagtgatcca cacccaacgc ctgaaatcag atccaggggg
     2461 taatctgctc tcctgattca ggagagctta tggtcacttt tgagacagtt atggaaatta
     2521 aaatcctgca caagcaggga atgagtagcc ggacgattgc cagagaactg gggctctccc
     2581 gcaataccgt taaacgttat ttgcaggcaa aatctgagcc gccaaaatat acgccgcgac
     2641 ctgctgttgc ttcactcctg gatgaatacc gggattatat tcgtcaacgc atcgccgatg
     2701 ctcatcctta caaaatcccg gcaacggtaa tcgctcg
//
LOCUS       DAZOZX010000277         2734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZOZX010000277 DAZOZX010000000
VERSION     DAZOZX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.282"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            88..153
                     /locus_tag="ACHUPT_004199"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHUPT_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACHUPT_004200"
     CDS             381..629
                     /locus_tag="ACHUPT_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0821501.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="ACHUPT_004201"
     CDS             complement(745..1014)
                     /locus_tag="ACHUPT_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0821502.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="ACHUPT_004202"
     CDS             1071..1739
                     /locus_tag="ACHUPT_004202"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0821503.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>2734
                     /locus_tag="ACHUPT_004203"
     CDS             2055..>2734
                     /locus_tag="ACHUPT_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0821504.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          835 a          589 c          538 g          772 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgtt
//
LOCUS       DAZOZX010000278         2714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZOZX010000278 DAZOZX010000000
VERSION     DAZOZX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.283"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            52..2574
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPT_004204"
     CDS             52..2574
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPT_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0821505.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2585..>2714
                     /locus_tag="ACHUPT_004205"
     CDS             2585..>2714
                     /locus_tag="ACHUPT_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0821506.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          585 c          679 g          723 t
ORIGIN      
        1 agtgacccga taaaaatgac gctgtaagca agggataagg ataggcgtat tatgatgacg
       61 accagaatag tggttggcct cacggcaggg acgtgtctga ttttctcgca aaacctgatg
      121 gccgaggtca gtgtattcaa tccggcgctt ctggaaatca accatcaatc cggagtcgat
      181 attcgccagt ttaatcgggc aaacctgatg cccccaggtg tttatagcgt tgatattttt
      241 atcaacggta aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct
      301 gatctgcacg cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt
      361 gatgcgctca aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt
      421 attgaaggct catcctggca gtttgacagt gataaattgc agctgaatat atccattcac
      481 caaatctaca tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg
      541 attaatgcgc tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat
      601 ggttcacaag agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg
      661 tggcggctac gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc
      721 attagtacct ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat
      781 acgtggacgg cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact
      841 gataacgata tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca
      901 aagtccaacg ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt
      961 ccacaaggtg cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac
     1021 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg
     1081 gcgattctta aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat
     1141 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
     1201 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
     1261 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
     1321 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt
     1381 gacgacacag acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat
     1441 accctcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct
     1801 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
     1861 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
     1921 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
     1981 tacagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     2041 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     2101 gccgccaggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     2161 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     2221 tcgaagtcga atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca
     2281 gtggttaaag cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat
     2341 gcaaatggta aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca
     2401 gattcaggaa ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga
     2461 caggttacgt tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcattt
     2521 tcgacaccag aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat
     2581 ttttatgaac aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag
     2641 tgtagcactt gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacagtac
     2701 gcttgatttc tcgg
//
LOCUS       DAZOZX010000279         2692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZOZX010000279 DAZOZX010000000
VERSION     DAZOZX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.284"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..893
                     /locus_tag="ACHUPT_004206"
     CDS             <1..893
                     /locus_tag="ACHUPT_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR0821507.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..2624
                     /locus_tag="ACHUPT_004207"
     CDS             894..2624
                     /locus_tag="ACHUPT_004207"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0821508.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT          757 a          661 c          707 g          567 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg
     2341 aagcaatcag gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa
     2401 aagatgctct tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc
     2461 tgatgaaacg aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa
     2521 aaattgatgc gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga
     2581 tgaaaaacct tgaccctatt gaaaagagag ttgaggtagc ctgagtttaa cggacactcc
     2641 ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc ca
//
LOCUS       DAZOZX010000280         2665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZOZX010000280 DAZOZX010000000
VERSION     DAZOZX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.285"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..357
                     /locus_tag="ACHUPT_004208"
     CDS             <1..357
                     /locus_tag="ACHUPT_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0821509.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="ACHUPT_004209"
     CDS             619..981
                     /locus_tag="ACHUPT_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR0821510.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1907
                     /locus_tag="ACHUPT_004210"
     CDS             978..1907
                     /locus_tag="ACHUPT_004210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0821511.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1904..>2665
                     /gene="gtrII"
                     /locus_tag="ACHUPT_004211"
     CDS             1904..>2665
                     /gene="gtrII"
                     /locus_tag="ACHUPT_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR0821512.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYN"
BASE COUNT          773 a          527 c          547 g          818 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggagaat atataggtag aatctatatt gaagtaaaaa
     1861 atagacccaa atacatcata aaaaaatctc atcgaggtaa cccatgatta aaattaattt
     1921 atttaaaaat gcaaatctcc ttgccttcat atcatgtttt gcaatatcaa tttattgtta
     1981 ttgggggtgg ttgtacgatg gaacacttaa tattgatggc gagtttacaa ataattttta
     2041 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccctga
     2101 gcctttttca ctttatataa caccattaat agccttatct tttatcatca tttcagcatt
     2161 tataatctgc agatcgctaa agctagaatc ttatgaatta ttgataggta tgttagtatt
     2221 tattaccttc cctcagatct cctatcaatt agagtttctt aaccaagctg atactgtggg
     2281 aattgctttt ctactggcag cgatatcagc aattattttt cactcgcaaa aaaataggat
     2341 tgtgatattt tctggtatag tactgtccat tctttcaatg gcaatctacc aaacattcgt
     2401 aacatatatt attgcattcg tcattgggtt gcagataaat tcgataatac gaaatgagaa
     2461 aaatattcgt gaatcttttt atagttcatg tttatctcta tccctcatag ctttatctac
     2521 cttaatttac ctgctattaa ccaaagctat caagcattat ttttcgcttg aatcgaacga
     2581 gtacatctca aattatatac aaaatgcaag cgatattaaa tggcttgtta aatcagccat
     2641 agataatata tataacttct ataac
//
LOCUS       DAZOZX010000281         2653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZOZX010000281 DAZOZX010000000
VERSION     DAZOZX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.286"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            137..1102
                     /gene="dusB"
                     /locus_tag="ACHUPT_004212"
     CDS             137..1102
                     /gene="dusB"
                     /locus_tag="ACHUPT_004212"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0821513.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1128..1424
                     /gene="fis"
                     /locus_tag="ACHUPT_004213"
     CDS             1128..1424
                     /gene="fis"
                     /locus_tag="ACHUPT_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0821514.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1510..2394
                     /gene="yhdJ"
                     /locus_tag="ACHUPT_004214"
     CDS             1510..2394
                     /gene="yhdJ"
                     /locus_tag="ACHUPT_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0821515.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2478..>2653
                     /locus_tag="ACHUPT_004215"
     CDS             2478..>2653
                     /locus_tag="ACHUPT_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0821516.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
BASE COUNT          733 a          623 c          649 g          648 t
ORIGIN      
        1 ttatcgcacc acgctcattt taaatgcaat tctttgatcc atctcagagg attggtcaaa
       61 gtttggcctt tcatctcgtg caaaaaatgc gtaatatacg ccgccttgca gtcacagtat
      121 ggtcatttct taactcatgc gcatcggaca atatcagctc agaaatcgcc tgatcgcagc
      181 gcccatggct ggcattacag acagaccttt tcggacgttg tgctacgaga tgggagccgg
      241 attgacagta tccgagatga tgtcttctaa cccacaggtt tgggaaagcg acaaatctcg
      301 tttacggatg gtgcacattg atgaacccgg tattcgcacc gtgcaaattg ctggtagcgc
      361 tccgaaagaa atggcagatg cagcacgtat taacgtggaa agcggtgccc agattattga
      421 tatcaatatg ggttgcccgg ctaaaaaagt gaatcgcaag ctcgcaggtt cagccctctt
      481 gcagtacccg gatgtcgtta aatcgatcct taccgaggtc gtcaatgcag tggacgttcc
      541 tgttaccctg aagattcgca ccggctgggc accggaacac cgtaactgcg aagagattgc
      601 ccaactggct gaagactgtg gcattcaggc tctgaccatt catggccgta cacgcgcctg
      661 tttgttcaat ggagaagctg agtacgacag tattcgggca gttaagcaga aagtttccat
      721 tccggttatc gcgaatggcg acattactga cccgcttaaa gccagagctg tgctcgacta
      781 tacaggggcg gatgccctga tgataggccg cgcagctcag ggaagaccct ggatctttcg
      841 ggaaatccag cattatctgg acactgggga gttgctgccc ccactgcctt tggcagaggt
      901 taagcgcttg ctttgcgcgc acgttcggga actgcatgac ttttatggtc cggcaaaagg
      961 gtaccgaatt gcacgtaaac acgtttcctg gtatctccag gaacacgctc caaatgacca
     1021 gtttcggcgc acattcaacg ccattgagga tgccagcgaa cagctggagg cgttggaggc
     1081 atacttcgaa aattttgcgt aaacagaaat aaagagctga cagaactatg ttcgaacaac
     1141 gcgtaaattc tgacgtactg accgtttcta ccgttaactc tcaggatcag gtaacccaaa
     1201 aacccctgcg tgactcggtt aaacaggcac tgaagaacta ttttgctcaa ctgaatggtc
     1261 aggatgtgaa tgacctctat gagctggtac tggctgaagt agaacagccc ctgttggaca
     1321 tggtgatgca atacacccgt ggtaaccaga cccgtgctgc gctgatgatg ggcatcaacc
     1381 gtggtacgct gcgtaaaaaa ttgaaaaaat acggcatgaa ctaattcagg ttagctaaat
     1441 gcttgattaa aaaggcgcta ctcggcatgg ggaagcgcct tttttatagg tgtcacaaag
     1501 ggagtgacca tgagaacagg atgtgaaccg acccggtttg gtaatgaagc taagaccatt
     1561 attcacggtg atgcctttgc cgaacttaaa aagctaccta ctgaaagcgt cgatctgctc
     1621 tttgccgacc caccgtataa catcggtaaa aattttgatg gtctgatcga agcctggaaa
     1681 gaagatctgt ttatcgactg gctgtttgaa gtgattgcag agtgccaccg cgttctgaaa
     1741 aagcagggca gcatgtacat tatgaacagt acggaaaaca tgccctttat cgatctccag
     1801 tgccgcaagc tttttaccat caaaagtcgc atcgtctggt catatgacag ttctggagta
     1861 caggcgaaaa aacactacgg ctccatgtac gaacccatcc tgatgatggt gaaagacgca
     1921 aagaactaca cattcaacgg tgatgctatt ctggtagaag ccaaaaccgg atcgcagcgc
     1981 gcgttgatcg attatcgcaa aaatcctcca cagccataca atcatcaaaa agtaccgggt
     2041 aacgtctggg attttccgcg cgtgcgttat ttgatggatg aatatgagaa ccacccgacg
     2101 caaaaaccga aagccttact gaaacgcatt attctcgcct cttccaaccc aggcgatatc
     2161 gttctcgacc cgtttgccgg tagctttact accggtgccg tagccatcgc cagcggacga
     2221 aaattcattg gtattgagat caacagcgag tacatcaaaa tggggcttcg acggctggat
     2281 gtcgcgtcgc attactccgc ggaagaactg gcgaaagtga aaaaaagaaa gaccggcaac
     2341 ctgtcaaaac gaagccggtt aagcgaagtt gaccccgatc tcattgcaaa gtaaaggaag
     2401 tgtaagccta gttttcagat tattcatttc gtgtatattt cctgccagac ttggttaaac
     2461 atgcacaggc tcaggtaatg attcgcaagt attggtggct cgtcgttttc gctgtcttcg
     2521 tttttctgtt tgatacttta ctgatgcagt ggattgaact gctgacaaca gaaacagaca
     2581 aatgccgtaa tatgaactca gttaatccac taaaactggt taactgtgac gaactgaatt
     2641 ttcaggacag aat
//
LOCUS       DAZOZX010000282         2650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZOZX010000282 DAZOZX010000000
VERSION     DAZOZX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.287"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHUPT_004216"
     CDS             <1..197
                     /locus_tag="ACHUPT_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0821517.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACHUPT_004217"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACHUPT_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR0821518.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUPT_004218"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUPT_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR0821519.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="ACHUPT_004219"
     CDS             1821..2576
                     /locus_tag="ACHUPT_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR0821520.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          648 a          620 c          666 g          716 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga
//
LOCUS       DAZOZX010000283         2599 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZOZX010000283 DAZOZX010000000
VERSION     DAZOZX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.288"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            101..1531
                     /locus_tag="ACHUPT_004220"
     CDS             101..1531
                     /locus_tag="ACHUPT_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0821521.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLAYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1572..2504)
                     /locus_tag="ACHUPT_004221"
     CDS             complement(1572..2504)
                     /locus_tag="ACHUPT_004221"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0821522.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
BASE COUNT          816 a          535 c          571 g          677 t
ORIGIN      
        1 aataaatata aatacattct gataatgcat cctgccgctg gacattattt cagcataagt
       61 ggatattcaa agttttgctg ttttatcagg gaatatttat atgatacgta agtcagctac
      121 aggtgttatt gttgcgttag ccgtaatctg gggtggtggc acatggtaca caggtacgca
      181 aattcagcct ggtatcgaaa agtttattaa agattttaac gatgcgaaaa agaaaggtga
      241 acatgcctac gatatgacgt taagttataa aaactttgac aaaggctttt ttaattcccg
      301 ttttcaaatg caaatgacat tcgataacgg tgcacccgat ctcaatatca agccaggcca
      361 gaaagttgta tttgatgtgg atgttgagca cggtccgttg cccatcacaa tgttaatgca
      421 tggtaatgtc atcccagcac tggcagcggc aaaagtgaac ttagtgaata atgaactgac
      481 acaaccgcta tttatcgccg cgaaaaataa atcgcccgtg gaagcgacat tgcgattcgc
      541 gtttggtggc tcattctcta cgacattaga tgttgcccct gcagagtatg gaaaattttc
      601 ttttggtgag ggccagttta cttttaatgg tgatggtagt tcattgtcta acctggatat
      661 tgaaggcaaa gtcgaagata ttgttctgca attatcacca atgaacaaag taacggcaaa
      721 aagttttacc attgattctc tggcgcgatt agaagaaaag aaatttccgg ttggtgaaag
      781 cgagtcgaaa tttaatcaga ttaacattat caatcacggg gaagacgttg cccaaatcga
      841 tgctttcgtt gcaaaaacca ggctggatcg cgttaaagac aaagattata tcaatgtcaa
      901 tctggcctac gaacttgata agttaacaaa agggaatcag caactcggta gtggtgagtg
      961 gtcattgatt gctgaatcta ttgatccctc agcagtgcgc caatttatca tccagtataa
     1021 cattgcgatg cagaagcagc ttgctgcaca ccctgagtta gcaaacgatg aagttgctct
     1081 gcaagaagtg aatgctgcat tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc
     1141 gaccattaaa caaccggtaa gatggaagaa cgcactcggc gaactaaatg ccaatctgga
     1201 tatcagtatt gccgacccag ccaaatcttc atcatccaca aacaaagata tcaaatcgct
     1261 caattttgat gtgaagttac cgcttaatgt cgtcacagaa accgcaaaac agcttaattt
     1321 atctgaagga atggatgcgg aaaaagcgca aaagcaggct gataaacaaa tcagcgggat
     1381 gatgacatta ggtcagatgt ttcagttaat cacgattgac aacaataccg cctcgctgca
     1441 gctgcgttat acaccgggta aagttgtttt taacggacag gagatgagcg aagaagaatt
     1501 tatgtctcgt gccggacgtt ttgttcatta aaatactcac tcgcctctgc tcattcagag
     1561 gcgagtaaaa cttacttatt ctgacgctgt gatgacatga gagacgtcag aagccggtct
     1621 ttttcctgcc acaatgagtt cagccattgc tgaaaatggc gcttaaagct tttgtcattg
     1681 atgtagtcgc catgtaactc atcggcaata ggctgtaaat ccacatgtac gacaatccgc
     1741 gtcagtttac cgcttaacat atcgaagaac ggctgacggt tattgtccgg ataacacagc
     1801 gtaacattaa gcagtttatc gaattgttta cccagtacat ttagcgccat cgcaatgcct
     1861 gccgcctttg gtggcaacaa gttttgaaaa gtggagtggg tttgctgatg tttttcttgc
     1921 gtgaaacggg agccttcaac gaaattgaca atggtggtgg gatgcaggcg aaacttttca
     1981 caagaacgcc gagtggtttc aacatcttga ccacgtcgct ccggatgacg taataaataa
     2041 gcgcgggaat aacgcttcat aaacggcata tccagcgccc agcacgccag gccaagaaat
     2101 ggcacccagg ccagctgttg cttgaggaaa tatttattca tcggaatgtg cttacgaaac
     2161 agcacgcaca gtacgacaat atctgcccaa ctacggtggt tacaaataag cagataccag
     2221 ttcttcttac ttaacccttc cagcccgtga acttcccatt gcaggtgtgg gttcagatgc
     2281 agtaataccg ccagaccttc acaccagcaa tacatcataa aatcacaaaa acgcgaaacc
     2341 tttcgccaaa tgactggcac aggtagcaat agttttacaa tcccggcaat gatgatcggg
     2401 acggaacaaa aaatagtgac caaaatggtc aatacgatac tcagcaaaag ggttatcgca
     2461 gcgagtattc tcgtcataat gaatttattc aaaaggttag ccatagacag tgcactaaag
     2521 aagcgcaagg cgcagatttt agcagaaaat gacccaaata acggatgatc cttaaggaga
     2581 aaaataattc atatctatc
//
LOCUS       DAZOZX010000284         2563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZOZX010000284 DAZOZX010000000
VERSION     DAZOZX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.290"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            77..1177
                     /gene="ompC"
                     /locus_tag="ACHUPT_004222"
     CDS             77..1177
                     /gene="ompC"
                     /locus_tag="ACHUPT_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0821523.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1341..1826
                     /locus_tag="ACHUPT_004223"
     CDS             1341..1826
                     /locus_tag="ACHUPT_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0821524.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            1936..>2563
                     /locus_tag="ACHUPT_004224"
     CDS             1936..>2563
                     /locus_tag="ACHUPT_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0821525.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDI"
BASE COUNT          840 a          514 c          471 g          738 t
ORIGIN      
        1 tatgtcattt ataaatgacg ccgtttaaaa ttcgtttaga aaatacgttt aacgataatt
       61 aaaaggattc tgttaaatga aattaaaaat agttgcggtg gttgtaactg gtttgttagc
      121 tgcgaacgta gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta
      181 tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac
      241 ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg
      301 tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaacaa
      361 gacccgtctg gcctatgctg gtttaaaaat tagtgaattt ggctctctgg actatggtcg
      421 taactacggt gtcggctacg acattggttc atggaccgat atgttgccag aatttggtgg
      481 cgatacctgg agtcagaaag atgtcttcat gacataccgt accaccggtc tagcaaccta
      541 tcgcaactac gatttctttg gcttaattga agggctgaac tttgccgcgc aatatcaagg
      601 caaaaataaa cgtactgaca acagtcatct ttatggtgct gacgacacgc gtgccaacgg
      661 tgacggtttc ggtatctcct caacttatgt ttatgatggc tttggtatcg gtgcggtata
      721 taccaaatcc gatcggacaa atgcgcagga aagagccgca gctaatcctc tcaatgcctc
      781 cggtaagaat gcagaactat gggccacagg tataaagtat gatgccaaca acatctactt
      841 tgcagctaat tacgatgaaa cattaaacat gaccacctat ggggatggtt atatctctaa
      901 caaagcacaa agttttgaag tggtggcgca atatcaattc gacttcggct tgcgcccatc
      961 actcgcttac ctgaaatcga aaggcagaga tctgggccgc tacgccgacc aggacatgat
     1021 tgagtatatc gacgttggtg cgacgtattt cttcaacaaa aatatgtcga cctatgttga
     1081 ttataaaatc aacctgattg atgaaagcga ctttacccgt gccgtagata ttcgcaccga
     1141 taacatcgtc gctacgggca ttacctatca gttctaacgt cctctcctga ccagcccttc
     1201 tctgggctgg tttttgattt aagtgctgct gtatgactcc ttaagtaaaa atgagcaatt
     1261 cttgatattt ctggtcattc acctcaatta atattattcc atatcctccg gggacatctt
     1321 actataatat ttattgatga gtgattatga ccaatattaa tacagcttgt gtaaaaaata
     1381 atgccagtta tcaacttaat aacgcattac ctaacaaaga aaccatctcc agcaattttt
     1441 gtgaacgact agaacaatgg ggtaataaat cggttaataa tggtgaagaa agagcaattg
     1501 ccgtggagcg aattaaagaa gcttacaatt cgaacatggc atctcttgat ttgtcatatc
     1561 ttgatttgag tgaattaccg cctatcccct cgacagttaa tacgttaaat ctggaaaaca
     1621 attgtctaac ttgtcttgac ttagctgaca atgccagtct cgttaatatc aacctcagcg
     1681 ttaataaaat taacacgata acctttccaa aagaaccaaa actggaaaac atttatatta
     1741 atcataacaa tctggaaagt ttagatttaa aaaatcagca ttcattggtt aatctggaag
     1801 cgcaaaataa tagcctaaaa aaatgatatt tctgatagtt ataaactgaa atttcttaat
     1861 cttgattata acaagctagc atcgctggat gtctcccggc aagaatctct gattgagtta
     1921 agtgcccatc acaatatgat cactgacctt atattacaca atcatcccag gatgaaaaca
     1981 atcactttaa acgacaacca tattgcacat ttaaacgcca aaaacactac aaaactggaa
     2041 tatttaaact taagcaataa caatttactg ccaaccaatg acattgatca actaatatca
     2101 tcaaaacatc tttggcatgt attagttaac ggcatcaaca atgatccact tgcacaaatg
     2161 cagtactgga ctgcagtaag aaatataatt gatgacacta atgaagtgac cattgattta
     2221 tcaggactta atttaaccac tcaaccacca gggctgcaaa acttcacctc tatcaatctt
     2281 gataataacc aactcacaca ttttgatgca accaactacg atagactcgt aaagctaagt
     2341 ctgaatagta atgctcttga gtcaataaat tttcctcaag gcagaaatgt aagtattaca
     2401 catatatcta tgaataataa tgctctcaga aatattgata tagataggct ttcatcagtt
     2461 acttatttta gtgcggcaca taatcaacta gagtttgtgc aattagaatc ttgcgaatgg
     2521 ctgcaatacc tgaatctcag ccataatcaa ttaactgata ttg
//
LOCUS       DAZOZX010000285         2543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZOZX010000285 DAZOZX010000000
VERSION     DAZOZX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.291"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            351..494
                     /locus_tag="ACHUPT_004225"
     CDS             351..494
                     /locus_tag="ACHUPT_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0821526.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            507..716
                     /gene="cspE"
                     /locus_tag="ACHUPT_004226"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             507..716
                     /gene="cspE"
                     /locus_tag="ACHUPT_004226"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0821527.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            882..1691
                     /gene="rlmA"
                     /locus_tag="ACHUPT_004227"
     CDS             882..1691
                     /gene="rlmA"
                     /locus_tag="ACHUPT_004227"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0821528.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1688..2254)
                     /gene="mntP"
                     /locus_tag="ACHUPT_004228"
     CDS             complement(1688..2254)
                     /gene="mntP"
                     /locus_tag="ACHUPT_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0821529.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2251..2373)
                     /locus_tag="ACHUPT_004229"
     CDS             complement(2251..2373)
                     /locus_tag="ACHUPT_004229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821530.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     regulatory      complement(2386..2476)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          677 a          603 c          616 g          647 t
ORIGIN      
        1 ttgatagccg ttaactgact gttttatgag aaaaagtgat ataacttttt attcattgca
       61 tagcagaaaa tgtgatattg cacgcactat gtaataactt ctccctctgg cctggaacaa
      121 ctgaacttat tgaactatgt tagaaactac gccagtttaa gtatctgcct gaactggcaa
      181 ggttaagcac aatgatatat cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga
      241 agacgtatcg agatttgtgc gtctgatcga gatatgttta aaaatggctt gccataatta
      301 acgttgtatg tgataaaaga tttcgggtta aacgaggtac agttctgttt atgtgtggca
      361 ttttcagtaa agaagtcctg agtaaacacg ttgacgttga ataccgcttc tctgccgagc
      421 cttatattgg tgcctcatgc agtaatgtgt cagttttatc tatgttatgc ctgcgggcga
      481 agaaaacaat ctaaggaatt tttcaaatgg caaagattaa aggtcaggtt aagtggttca
      541 acgagtctaa aggttttggc ttcattactc cggctgatgg cagcaaagat gtgttcgtac
      601 acttctccgc tatccagggt aatggcttca aaactctggc tgaaggtcag aacgttgagt
      661 tcgaaattca ggacggccag aaaggtccgg cagctgttaa cgtaacagct atctgatcga
      721 atccactgat ctgaagtgtg aatacgcttc aatctcgcta taaagcctcg tcgaatgcga
      781 ggctttttac tatgctttat cttcgctcct ggcgttcgga tatttgcccg ccgcgtgatt
      841 cgcgttacac ttgcggcctt tagtatcctg ccggagttgt catgtcgttt tcctgtccac
      901 tttgccatca gcctctttcg cgtgaaaaaa acagctatat ctgtccccaa cgacatcagt
      961 ttgatatggc gaaagaaggg tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg
     1021 atccgggcga cagcgcggaa atgatgcaag cacgccgcgc attcttagat gccggacatt
     1081 atcagccgct gcgtgatgca attgtcgccc aactgcggga acggcttgat gaaaaggcca
     1141 cggcggtgct ggatattggc tgtggtgaag ggtattacac acacgcattt gccgatgcgt
     1201 tgcccgaaat caccacgttt ggcctggatg tctcgaaggt agcgataaaa gcggcggcga
     1261 aacgctatcc gcaggtcact ctttgtgtcg cttccagcca ccgtttgccg ttttccgata
     1321 ccagtatgga cgccataata cgtatttacg cgccgtgtaa agcagaagaa ttagcacgag
     1381 tagtgaagcc cggcggctgg gtcattactg ccacgccggg accgcgacat ttgatggagc
     1441 tgaaggggct gatttacaat gaagtacatc ttcatgcacc tcatgcagaa caactggaag
     1501 gttttacatt acagcagagt gcggagttgt gttatccgat gcgtcttcgc ggtgatgaag
     1561 ccgtcgcatt attgcagatg acgccgtttg cctggcgtgc gaagccagaa gtctggcaaa
     1621 cactggcagc aaaagaagtg ttcgactgcc agacggactt taatattcac ctctggcagc
     1681 gttcttatta accgtggaag tgcgtccaga ggatctggac gccgatgccg atcagcacca
     1741 gcccgccgag aatttccgct tttttcccaa taattgagcc gataaagcga ccaaccatca
     1801 tccctaatgt cgacataatc aaggttgcac aaccaatggc caatgcggtc gcgataatgt
     1861 tgacctgaag gaaagcaaga ccaacaccca cagccatggc atccaggctg gtggcaatcg
     1921 cggtggttac cagtagccag aaaccgtgcc gacggcgcgg ctcttcatct tcatcatctg
     1981 cgccacgaaa accctcaata atcattcgcc cgccgaggaa tatcagcagc acaaacgcaa
     2041 tccagtgatt ccattcaagg acaaaccggc tggctaacat gcccattccc cagccgatca
     2101 gcggcgtcag ggtttcgacg gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt
     2161 tcggtttatg gaggggggca cctttaccga ttgatgcagc aaatgcatcc atcgacatac
     2221 caaacgcaag aagaacagta gcagtgatat tcatgacaac gtcctgaccg gggtatccat
     2281 aaaacacatc atcgccccca gcaaacatgc atacatgaac aacaagccgc gatgggtttt
     2341 cgcgttcatg caggcatagc gattgatgtg catatggtct cgcctgactg aaagtcttca
     2401 gtccgtacgt gccacgtttt gcaacgagta tgttgacacg tacatttccg gaatctggaa
     2461 atcggctact ccccaaatga gggcgcaacc ttaacatatt ttgagaatat aaaacaactg
     2521 ttggaatata ttatttcatt gct
//
LOCUS       DAZOZX010000286         2491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZOZX010000286 DAZOZX010000000
VERSION     DAZOZX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.292"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(220..384)
                     /locus_tag="ACHUPT_004230"
     CDS             complement(220..384)
                     /locus_tag="ACHUPT_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821531.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACHUPT_004231"
     CDS             1286..1570
                     /locus_tag="ACHUPT_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0821532.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACHUPT_004232"
     CDS             1558..2043
                     /locus_tag="ACHUPT_004232"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0821533.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            complement(2216..>2491)
                     /locus_tag="ACHUPT_004233"
     CDS             complement(2216..>2491)
                     /locus_tag="ACHUPT_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFR0821534.1"
                     /translation="ASKSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANS
                     IKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          648 a          529 c          688 g          626 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataaggg ctgagggatc cccacaaaat tactgctaat
     2221 aaaccagcac catatttcgg taggttctgg acagatggtt tagaactgtg ctcagtactg
     2281 ttcgtcttaa aataagtggc gagtgtcgaa gaacattctt cgctaacgtc agatatgaga
     2341 taacccgccg ggatttaatg ctgttagcct gatatttcag gtgtaaccct ttattttcag
     2401 cgtgataacc gagtaaccac attacgatag tgctcaacgt tgccagcaga ctcagaacca
     2461 gaattcttcc tgttgaacga cttttgctgg c
//
LOCUS       DAZOZX010000287         2487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZOZX010000287 DAZOZX010000000
VERSION     DAZOZX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.293"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>2487
                     /locus_tag="ACHUPT_004234"
     CDS             <1..>2487
                     /locus_tag="ACHUPT_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0821535.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DAZOZX010000288         2452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZOZX010000288 DAZOZX010000000
VERSION     DAZOZX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.294"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUPT_004235"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUPT_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUPT_004236"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUPT_004236"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0821536.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHUPT_004237"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHUPT_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT          687 a          631 c          529 g          605 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aa
//
LOCUS       DAZOZX010000289         2450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZOZX010000289 DAZOZX010000000
VERSION     DAZOZX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.295"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(38..583)
                     /gene="ygeQ"
                     /locus_tag="ACHUPT_004238"
                     /pseudo
     CDS             complement(38..583)
                     /gene="ygeQ"
                     /locus_tag="ACHUPT_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
     gene            complement(1245..2234)
                     /locus_tag="ACHUPT_004239"
     CDS             complement(1245..2234)
                     /locus_tag="ACHUPT_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821537.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2318..>2450)
                     /locus_tag="ACHUPT_004240"
     CDS             complement(2318..>2450)
                     /locus_tag="ACHUPT_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821538.1"
                     /translation="REQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          653 a          560 c          589 g          648 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacag
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctgag ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg agcctacgac aaatcgaaga
      841 caaccagcta acctattgtt tcttaaagac aggtgaatcc agttggttgt agtgatagtc
      901 acacatttag agatagaagg tcaagacttg aagcacgtaa tattccctct aaaggaatag
      961 aactcacggt ctgactaccc ggcccttttg gtctggctca ggtcaggcta tgggggcttt
     1021 tggctcgctc tttatattag atgaggtctc acatttttca gtcaaaactc atcgtctaat
     1081 agcccatagc cagaccacca gtcccagctt tagcaggaag accacgagat agactttgtg
     1141 taggtccatt tggccctcct actttctgta ttcacaaact agattactag tacaaaataa
     1201 aggccagaca tccagttcgg aagtctgacc aatagtcgtt attgttagtt agcttcttta
     1261 gtgaagctca ggcgtttgac tacaggcagg acagcagtag ccgagccagt acccatcttg
     1321 atggtcgtag cagcttcagc tacaacaaac accttgccga gaatctcaag gtcgtgagac
     1381 tgggagtctc catcacgtga aaggaccaca gtaatgctct cgccatcgtg acggaagcaa
     1441 ccgaagctca gagccgtaaa acgcttagcc atcatgaaca cgccacggtc ttcaccgttg
     1501 taataggcga cgtcagtccc cagcatatcg acgatagcgt cttcaccacc gaagccctgc
     1561 ttagcagcca gacgggagag agccttgagg tgtgactcat gggcgataat gccgtagtca
     1621 gaaacctcag aaccgaaaga agactccgtg ttctcgtaca gggcatccat gatagcgtca
     1681 gccttagcgg tcaggctacc agtcagggcc ggaagttcta cggtcttcag cagctttgag
     1741 gactccagca cgtcaaccat cttagagtta atggtacgaa tcacgttatc gcagatgttc
     1801 agtgcgtcct gctggagcgt cccctgagtg aaaccagtgg tcgacttctg gtcccaaagc
     1861 tttgtttggt gagacacgag gatcgcctgt tcaatcggct caacggtgat ttcagtgcca
     1921 gacggagcgg tcacaacttc aggtcgctta ccaccacgga cagggaccag cacagagcgg
     1981 tcagccagag tgtgagcaat gattggagtc ggtaagctgt tacgggtcgg caggacttct
     2041 acagggaacg ggatgttatc ttgagcgtct ttaagtacac cgttcagcat agccttaaca
     2101 gtcttagcta actggagggc atagacctga gggtcggcgt tagtcggcag accttcattg
     2161 acctgaatgt tgtcggtatc ggtgatgcgt actggctcaa tgccaaactg gaaagcgttt
     2221 ttagtcatat tcattataat taatctcctt gagtcaaata cttgaagtcc ctaacaggca
     2281 gtcagggacc gtgtgttctc agatgaggga cgcttgctta caagctgtcc atccagttac
     2341 cgcctgactc aaggtcagcg acgatagcgg cacgtgagcg accagcggaa tgagtagcgc
     2401 tgcggaggtt ttcaccatag gtccggcgtt cagctttgac ctgttcgcgg
//
LOCUS       DAZOZX010000290         2439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZOZX010000290 DAZOZX010000000
VERSION     DAZOZX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.296"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..565
                     /locus_tag="ACHUPT_004241"
     CDS             <1..565
                     /locus_tag="ACHUPT_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821539.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="ACHUPT_004242"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="ACHUPT_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0821540.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHUPT_004243"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHUPT_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0821541.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="ACHUPT_004244"
     CDS             2167..2403
                     /locus_tag="ACHUPT_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0821542.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          534 c          627 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DAZOZX010000291         2436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZOZX010000291 DAZOZX010000000
VERSION     DAZOZX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.297"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHUPT_004245"
     CDS             <1..360
                     /locus_tag="ACHUPT_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0821543.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHUPT_004246"
     CDS             361..726
                     /locus_tag="ACHUPT_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0821544.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHUPT_004247"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHUPT_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHUPT_004248"
     tRNA            1605..1680
                     /locus_tag="ACHUPT_004248"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHUPT_004249"
     tRNA            1682..1758
                     /locus_tag="ACHUPT_004249"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACHUPT_004250"
     tRNA            1761..1836
                     /locus_tag="ACHUPT_004250"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACHUPT_004251"
     tRNA            1840..1914
                     /locus_tag="ACHUPT_004251"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="ACHUPT_004252"
     CDS             2207..2422
                     /locus_tag="ACHUPT_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0821545.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          520 c          653 g          623 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcatta
//
LOCUS       DAZOZX010000292         2400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZOZX010000292 DAZOZX010000000
VERSION     DAZOZX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.298"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..237)
                     /gene="rfbG"
                     /locus_tag="ACHUPT_004253"
     CDS             complement(<1..237)
                     /gene="rfbG"
                     /locus_tag="ACHUPT_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR0821546.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGK"
     gene            complement(230..1120)
                     /locus_tag="ACHUPT_004254"
     CDS             complement(230..1120)
                     /locus_tag="ACHUPT_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR0821547.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1150..>2400)
                     /gene="wzx"
                     /locus_tag="ACHUPT_004255"
     CDS             complement(1150..>2400)
                     /gene="wzx"
                     /locus_tag="ACHUPT_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR0821548.1"
                     /translation="IIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIAL
                     VGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSDGI
                     VNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSSCI
                     AGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLFFF
                     GGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPR
                     LSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNILNV
                     LLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISW
                     SIRVILDALLLQWLYTRMCSVYEN"
BASE COUNT          845 a          460 c          338 g          757 t
ORIGIN      
        1 tttacctgcc cccccaaact tattgacgct tggaagtata tttataattg agggatatga
       61 taattgaaat tcttcacaaa tatttaatgt attatcagtt gataaatcat ctgatataaa
      121 aattgagatg tctacatctt tttgttcaat aatacttttt aattgttcct caatccagca
      181 ttcgccatta tacgtagcca taagtacagc tactcttttt tgatcagaac tatgcattat
      241 aacgaccgcc cccagtaatt cctcttattc catccagcgt agccaaaata tgttttttca
      301 tatattcaat tttatttttt tgagttgcga taattaaagt cgtatgaatt aaagaaaaaa
      361 caacttccct aatagagagt aaaagaggca catgattttt tctaagcaaa agaaatgcat
      421 ttcttactcg ataataacga cgtactggcg agtgaattgg tatgttaatc ccacagattt
      481 ttactgactt atccccaata gcatgaacca tttctatatc aggaatgata tgaactaata
      541 tgccatagtt taagcatcta agacaccatt ctgtatcgac ataatcaata aaaagttcct
      601 catccatcat tccaacaatc tcaaaaactt cttttgaaac catggttccg gatgagattg
      661 ttactgaact tttaaacggt gtctgtcctt cagtgacatg aattttctct cgaagaccat
      721 tttttttgat attacaaata ggataataat gtgacttatc tctatcaata aaaactgggc
      781 cagctatttt tgcattttca ttatccatat agcaggaaag ttttttgata aactcattgc
      841 gaatacggct atcttgatca aaaaaaatta tcttctctgc ccctttttca agagaatatc
      901 gaatcccaat attttgtgct tttgctatac cttcattcct accaagacaa attaaatgaa
      961 ctttttcaat gtctgctaat ttatctttca aggtgaaatt agtcctatta tcgactacta
     1021 caacgtaaca attttgctcc tttagttcag tgatcagtgc attcagattt ttaagttcgg
     1081 gattatatgt cacaatgaca gcgtaaatat tactattcat tatccaagtg actcagtaat
     1141 tggttaattt tagttttcat atacagaaca cattctagta taaagccatt gtaataacag
     1201 agcatctaaa attacacgta tgctccagga tattgcggca cccagtaacc catatttttc
     1261 gaccataaaa tacaaaaggg ctaaataagg aaccaattca gcacaatgaa tcaaagcagt
     1321 aattttagat tttcctaatg cctggattgc agagaatggt atcattgcca gcgcattgaa
     1381 gaaaaaaccg atcaataaca catttaatat attaaaggaa ttaatttgat aattctcacc
     1441 aaaccatatt ttcaatacaa gccctgaata taacaagcct atgattatca ccggcaaaca
     1501 gattagaaac attaaaagca atgatttatt tacattacgt ttaaattctt taaagtcttt
     1561 gatgttactt aaccttggaa acactgccct cccgattgct gcaggtatta ttcctaattt
     1621 tagaataacc tccgctggtg cagaataaaa tgcaacttta tctgccccca taatatttga
     1681 cactataaat cgatcaaaat acaccatgac cggacttatg atattactta ctgtcatcca
     1741 accgccaaag aaaaagagtc gtttaaaggt tttataacaa aaatgaaccc ccgaaatttt
     1801 aatatcattc ctgacataat aggcagagac taaaatcgaa ataactcttg caaatattaa
     1861 accagcaacc gctgccgaca atgtagcact ataaaaaaca aatatggccg gaattcccgc
     1921 gatgcaagag cttgatatag atttttgaat atttacaatg ccaaattttt catccccctc
     1981 aagaatggct gaccataatt gatttagaat aaataatgga atgcaaatag ccaacagttt
     2041 aaatgctagt tgtatatcac tatgttcaac accagaaatt tttagataat taacaattcc
     2101 atcagagaaa atcaataata aaaaagcacc aaagcatgaa aatagcacca aaaaagatgt
     2161 acttgttgaa attaccttgg ttctttcatg atgattatca cgatgaattg caatttctct
     2221 aataacagag cgagtcaggc ctacatcaaa aattccagca tacccaacaa gtgcaattgc
     2281 aattgtataa acaccgaaac cttcaggccc caaacctcga gcgagaaatc ctagcgaggg
     2341 gatagcaatt aaagttggta ttgcatagcc aaaaaggttc cagacactat tttttattat
//
LOCUS       DAZOZX010000293         2379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZOZX010000293 DAZOZX010000000
VERSION     DAZOZX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.299"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(662..2266)
                     /locus_tag="ACHUPT_004256"
     CDS             complement(662..2266)
                     /locus_tag="ACHUPT_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0821549.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          624 a          562 c          513 g          680 t
ORIGIN      
        1 taattaaatc ttttatttaa tacttaaagt taatctctaa ttgtgtcttt aagttaactt
       61 taagtaatac tataagttaa tctttaattg tgtctttaag ttaactttaa gttaagcccc
      121 tgtagggact agagaccata attaagatga ttgtgtctta ttgctattca cttatcgact
      181 gacccagagc acacaggaag cattcacttt aagtagaacg cttggtgtct gtaattggtc
      241 atcccgcccc tgaaaggatg cgataagtta cggttagttg tcctagaaga gaaaactttt
      301 tgtgtccctt ctccctatag tgagtcgtat taattatcct tgacaagtag agaaaaccat
      361 gagtcaaaaa catgaccaca caggtctgta gtaagtgtca tcctagaaga gaaaactttt
      421 tgtgtccctt ctccctatag tgagtcgtat taattacccc ttgacaagca gagaaaacta
      481 tgagtcaaaa acatgaccgc acaggtctgt agtaagtgtc atcctagaag agaaaacttt
      541 ttgtgtccct tctccctata gtgagtcgta ttaattatcc cttgacaagt agagaaaact
      601 atgagtcaaa aacatgacca cacaggtctg tagtaagtgt catcctagac tatggtctcc
      661 tctaggtctc cttataaaca tcagccatca cattgaccag cctatcaacc tcgacctctt
      721 tagcataacg gtcgtacgta atagaccctg aactatgccc catgatcgcc tgtgcatacg
      781 ccaactcgac cccatgttgt tttaactggg ccgcaactgt atgtctaaac gagtgaaacg
      841 ctaagccgct acggtctacg aggactttag gcaacaacgt ccgattaaac catttactca
      901 cctgctcacc gtaaccgttc ttcatgagac gcagaccatc gaacaccata gcgtcatcac
      961 cgtcagcgcc tctacgggtc tctacaagcg ccataaagtc agccaatata aaaccatagg
     1021 ccccatcgac cagaggcaca cagcggtcac tatgagcgtt cttgatgctc ttaccgggta
     1081 gactactatc gtcttcattg atatgaatat aaacggttcc cgcctcagtg gtcctgacgt
     1141 ctttgacctg tagttgggct atttcattga gtctcgcccc agtgatagca gccaaggcgg
     1201 taacataata atgatacggc ttaccagagg tcttctgaga gtatgcctta gctgcgacca
     1261 gtagttgccc cacctgctcc gtagagaagg cgttacgggc ccctgaggct ttacgctgag
     1321 ggactttaag ctccagaccc tcagtcatgt tctttttaat caagtcattg cgcacagccc
     1381 acctaaagac agcagccatc ttaataagat acttgttgtt gatggtaacg acatccagac
     1441 aatctgtctt atcttcacgg ctcaacaggt cagcaagtgg gacatccttg aagcgctgct
     1501 tacggttcct aggtaactgc tggagcacat cacggaccct aagcatgtca gccctgttag
     1561 cgtctgcatt gagacccaga tggtcgaata tttcgattaa cgcagcgtgt gacgccttgt
     1621 tttcccttaa ggttgcaggc ttccagttct gatagttctc cgcttcgtat tgttcaaaga
     1681 ggctcgctat ggtaatgcta ggcgtaactt ctggttttac ttctggagca ttaacagata
     1741 gggacacaga gtctgcttgg ttatcttgcc tatcaatatc attaaaaaga tcgacgtaat
     1801 ctatcagagg ctgactgtct cccgcttcga tacgcctcat acaggctttt agcaccttca
     1861 gggcctcatt gatgtaacga tgctggtcaa tgcttagcgg ctcactagcc agagcatcgg
     1921 taaggctctc accaagttct ccgtattggt cccggtagat atccctcatg tcaggctcaa
     1981 agagatctga acggcccata gacagttcac actcagcgat gtccttaagg tgttccctca
     2041 tttcctcata ggtagcgttg ggtctgtctg cgtgtattgc cttgagtgct gcctttatgt
     2101 gccttgagta agccatagcg gtgcgtctat ccttggtccg taatgatatt gaggtcattc
     2161 tatcattgtt tgattgtctg accctaagac ggaagtagta gacgccgtta cgctggtaga
     2221 gatagtagac tgctggaagt ttgtgactac tggatagctg aatcatatgt ctgcatgccc
     2281 tgagagggtt acacagtggg ttacagtaag taactctgca aggaggctta aagccgtatc
     2341 ccttaggatt cagtaggttg gagtacttta agaaaggtc
//
LOCUS       DAZOZX010000294         2374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZOZX010000294 DAZOZX010000000
VERSION     DAZOZX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.300"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..415
                     /gene="torD"
                     /locus_tag="ACHUPT_004257"
     CDS             <1..415
                     /gene="torD"
                     /locus_tag="ACHUPT_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0821550.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(508..813)
                     /gene="cbpM"
                     /locus_tag="ACHUPT_004258"
     CDS             complement(508..813)
                     /gene="cbpM"
                     /locus_tag="ACHUPT_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0821551.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(813..1733)
                     /gene="cbpA"
                     /locus_tag="ACHUPT_004259"
     CDS             complement(813..1733)
                     /gene="cbpA"
                     /locus_tag="ACHUPT_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0821552.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1994..>2374
                     /locus_tag="ACHUPT_004260"
     CDS             1994..>2374
                     /locus_tag="ACHUPT_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821553.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSE"
BASE COUNT          615 a          559 c          584 g          616 t
ORIGIN      
        1 cacgctgaca gtacgtgacg atgcccgtct ggaactggcc gctgactttt gcggcctgtt
       61 tctgatgacc gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac aggacgagca
      121 agagattaaa cgcttgttag ttgaggcagg gatggaaacc agtggcaatt tcaacgaacc
      181 ggcagatcat ctggcgatct atctcgagtt gttgagttat ctgcattttt cgctgggaga
      241 ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa aaaacactga cggcgctgcg
      301 gcaatggtta ccagagtttg ctgcgcgttg tcgacagtat gacagctttg gtttttacgc
      361 ggcactaagc cagttattgc tggtgttagt ggagtgcgac caccaaaaca gataacgtcg
      421 tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc attatggcgc agttgccgga
      481 tgcgtcatca catccggcaa acacacatca cggatgagct acaaaccggg aaagccgctg
      541 gcgtagcagg cggttttcct gcttcaggtg cgcaatatca tccattaacg tcagcgccac
      601 cgcgatcccc ggccagtcca gagccagttc atgacgcagg cgtaccgcgc gttgcaccac
      661 aatggcggca tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg gttcaaccac
      721 ccccaaaccg acaatttcat tcaactcctc ttcagagatg ccggtatgca ggcaaaattc
      781 ggtaatagta aaagtcaccg taacattagc cattatgctt tcccccaatc tttacgtgga
      841 tcaaaagacg actgggcgtc tgccagttgc tgccacagcg cggcagtgtt ttcatccggt
      901 ttcggcggca tcacgatttt cagtaccgca tacagatcgc cggtctgttt tttgctcacc
      961 agacctttgc ctttaacgcg caatcgttgc ccggcctggc tgcctggcgg gatagtcagc
     1021 aaaatgcttt ctttcagtgt tggaacggtg actttagtac ccagcgccgc ttcccacggg
     1081 ctaaccggca ccacaatttc cagatcctgg ccgacaatat cgaacagcgg atgtggcgca
     1141 atatgaatca ccagccacaa atcgccattt ggaccgccgt tttcgcccgg cgtcccctgg
     1201 cctttcaggc ggatgcgttg accattgccg acgcctgccg ggatcttcac attcagcgtt
     1261 ttcgggattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt atagctgatg
     1321 gtacgcttat gctcagtaag cgtttcttcg aggaataccg ccacttcgat ttcaatatcg
     1381 tggccacgtg cggcggggcg ttgacggctc tggcgggcat gctgaccgaa aattgacgag
     1441 aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt cgccatgatg gaactgacgg
     1501 ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg acgttgttca
     1561 tcacttaaca cttcccaggc ttcagcgacc tctttgaagc gggcttcggc atccggttct
     1621 ttgctgacat caggatggta tttacgggcc agtcgacgat aggcggtctt gattgtcttg
     1681 agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc catagcgtta
     1741 tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta taagtgtagg
     1801 gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat cgcggagagg
     1861 aggtcgccat caagatgggt tgctgaacat attttaaaca ggtgaaaaag ggtgagcgat
     1921 ttttgatagt tgaaccaggc actttaagtt taactagggc gtcattattt attaaatttt
     1981 atagacgcta tatatgggta gtaatataca tggaattagt tgcactgcaa ataattattt
     2041 gaaacaggcc tggaacgata taaaaaatga gtacgaaaaa aatcaaacat attcaatcac
     2101 gctttttgaa aacacactgg tgtgttttat gcggttgtac aatgaactca gacgtaaagt
     2161 aaatgaagag gatactccat gtctggaatg tgaatcacta gaaaaagaat ttgaggaaat
     2221 gcagaatgat aatgatctat cattatttat gagaacattg cgtactaatg atacacaaat
     2281 ttattcaggg gtttcaggag gtattacata tactatacaa tatgttcaag atgttgatat
     2341 tgttagagtg tccttgccgg gcagaggttc agag
//
LOCUS       DAZOZX010000295         2353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZOZX010000295 DAZOZX010000000
VERSION     DAZOZX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.301"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="ACHUPT_004261"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="ACHUPT_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0821554.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="ACHUPT_004262"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="ACHUPT_004262"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0821555.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="ACHUPT_004263"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="ACHUPT_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0821556.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          530 a          606 c          553 g          664 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac atcggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat
     2281 agtgtctgga agtattattt ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt
     2341 gattgccagc gaa
//
LOCUS       DAZOZX010000296         2325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZOZX010000296 DAZOZX010000000
VERSION     DAZOZX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.302"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHUPT_004264"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHUPT_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0821557.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHUPT_004265"
     CDS             282..452
                     /locus_tag="ACHUPT_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR0821558.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHUPT_004266"
     CDS             527..802
                     /locus_tag="ACHUPT_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821559.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHUPT_004267"
     CDS             902..>2325
                     /locus_tag="ACHUPT_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR0821560.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZOZX010000297         2311 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZOZX010000297 DAZOZX010000000
VERSION     DAZOZX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.303"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..433)
                     /gene="mxiG"
                     /locus_tag="ACHUPT_004268"
     CDS             complement(<1..433)
                     /gene="mxiG"
                     /locus_tag="ACHUPT_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR0821561.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLS"
     gene            complement(442..900)
                     /gene="ipgF"
                     /locus_tag="ACHUPT_004269"
     CDS             complement(442..900)
                     /gene="ipgF"
                     /locus_tag="ACHUPT_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR0821562.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(900..1262)
                     /gene="ipgE"
                     /locus_tag="ACHUPT_004270"
     CDS             complement(900..1262)
                     /gene="ipgE"
                     /locus_tag="ACHUPT_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR0821563.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(1272..>2311)
                     /locus_tag="ACHUPT_004271"
     CDS             complement(1272..>2311)
                     /locus_tag="ACHUPT_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0821564.1"
                     /translation="CASTRESDHIANMWLSKVVDDEGKEIFSGIRHGVISAYGLKKNS
                     SERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKIVSTSLLTPTSLTGGEESML
                     KDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVVTFNFGVNELALKMGLGWRN
                     VDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKNDVIYLANQIKEIVTKKLQK
                     NDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQDAEIKREIIRKHETGQFSQL
                     NSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKKLPLSSLELSYSERIGDPKI
                     WNMVKGYSSFV"
BASE COUNT          689 a          529 c          296 g          797 t
ORIGIN      
        1 aagagaggga aaaaattata attaacacaa caataacagc aaagaaaaaa aacaccgagt
       61 ggttcttata cattccgttt agtatatggc ctctcgattt atcctccctc atatttttaa
      121 ggtgaaaaga aatcccgtca tattcaaatg aattaaattg tgaattgata tcaatttgct
      181 cacctttttc atcactaaat tgacagatat tatctttgct tatggtcaaa taaattatac
      241 catccgattt actatctgtt acaggaataa tattttctgg gaaattgtcg tttccaaact
      301 caagtgtttc tatcgtccgt ccgagaacaa ttaagttatt cccataatat aacggaaact
      361 catccccgac accgtttgtg agtttcacta ataacctgaa tggtgcaaga tttgagttct
      421 ttgcctcaga cataaatcct cttatatcct tcgattattc tgcttgctct ctgctgctat
      481 ccgcaaatag cgtctataga tatcctctgc gtactttaat ctttcttttt tctttttcgg
      541 agacgttcct gcattatagg ctccaacagc ctcccaccct cttccataca tcatcataaa
      601 ctcatttaat aactttgctg cataatgtac agaaagacat ggatgactaa ttagcatttc
      661 ctcagaatat cccatttctc taagtctttt agaatgaaag tcatttattt gcattatacc
      721 ataatcttta cttccattgt tattaacatt gactgcagat ttattaaatc cggattcttt
      781 ttccgcaatc gcttttaaca aacttgatgg aatattgtac ctttcaccag ccttatccca
      841 acaatcagct ctccctaaat atgggataaa acacagcaaa ataaatacga aacgggacat
      901 taatacccct tcattcttcg cgcaaattca tccttcagcc aacgcacacg cgaaatataa
      961 tattctgtat taacatatac atcctcgaat tcatttatcc cggtaagatc taatcgcgca
     1021 attagatttc cgccttcatc atcagtagct aagcatatct tttctgagta atttaggctc
     1081 aacgcatata taagatcatt tatattttca ggcaatgcac aaaaagggca taataaattt
     1141 attgaatctc cttctttttc aatatatatt agcacatcat catcaagcat gattgcttga
     1201 tcatcgatat caattaaagg gatacccaag gctcggcaaa taacatctgc taaatcttcc
     1261 atatattcct cttatacaaa tgacgaatac cctttcacca tattccatat ttttgggtcc
     1321 cctattcttt cagaataaga tagctctaac gaagatagcg gcaatttttt catgacttta
     1381 tttccgggca caccagtatt catctcttgg atttccatat taccactatt cattagaata
     1441 gtagaaaata atcttttctc ttctgaggat aatttactat ttaattgaga aaactgacct
     1501 gtttcatgtt ttcttattat ttctctttta atttcagcat cctgcatacc tgttctgtcc
     1561 ttcccactct tacaattcca gcaaggtaca gcacctatag tataagccaa tagagtcatt
     1621 ctttgtgaca atttatacgg ctctccatta tcattttttt gtaacttttt ggttacaatc
     1681 tcttttatct ggttagccag atatatcaca tcattcttac atggtggatt tttttctatt
     1741 gcttctgcag cccaacctcc aatcacacca tttttgagga aattatcccc caacaaagaa
     1801 catattgatt catcattaag cttgtcaaca ttcctccagc ctaagcccat ttttagcgct
     1861 aattcattta caccaaagtt aaatgtcacc acttttagat taacgcttac ttctttcaga
     1921 agaccatcac tattccgaat taataatttc gttggcttcc cccttttaga gtttaaccct
     1981 tttaatgcgt tcacctgatc ttttaacata ctctcctctc ccccggttaa actggtcggc
     2041 gtcagaagag aagtcgaaac aatctttaaa tctactgttt taccagacaa agcctgtgat
     2101 aataactctg gtctgctata taatgctgcg cttactaact cctcagcttt attacgagca
     2161 gcaacagctc tttcagatga gttttttttc aatccatagg ctgatataac tccatgtctg
     2221 atacccgaaa aaatttcctt cccctcatca tccaccactt ttgaaagcca catattcgcg
     2281 atatgatcac tctctctcgt agaagcacaa c
//
LOCUS       DAZOZX010000298         2269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZOZX010000298 DAZOZX010000000
VERSION     DAZOZX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.304"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..94
                     /locus_tag="ACHUPT_004272"
     CDS             <1..94
                     /locus_tag="ACHUPT_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0821565.1"
                     /translation="NVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(183..695)
                     /locus_tag="ACHUPT_004273"
     CDS             complement(183..695)
                     /locus_tag="ACHUPT_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0821566.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            890..1654
                     /locus_tag="ACHUPT_004274"
     CDS             890..1654
                     /locus_tag="ACHUPT_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821567.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1605..>2269
                     /locus_tag="ACHUPT_004275"
     CDS             1605..>2269
                     /locus_tag="ACHUPT_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0821568.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          649 a          495 c          573 g          552 t
ORIGIN      
        1 aaacgttctc ggtatccggg acggaatcat cagcgaccag acagcattga gaatgcttca
       61 ggagtacatc aggatccaat gccttggggg gtagcggtaa ttttactcat tatccttcaa
      121 atcagattct gttgtcagag gaatggggga agctggattc gcaagagctt tttatgggca
      181 gtttagctaa aaaaaggcaa cacgaagcta aaactaccaa cataaagtgc tgaccagcta
      241 gcagctgaaa tcagactcac tgtatacact ttcactggat gaagttgcaa cattcctgcc
      301 accaaaggag caatgtaacg cagcacggcg ataaagcgcg aagtgaaaag tattgacaca
      361 gagttatttt gcaactgaag gcgaacccgt tctattgtat tggaacggct cgatattata
      421 catgcaagtt gagggatatg cccaattatt ataccaagat ggtaattaac gatagtaccg
      481 caccatgcgc cactcatgac cgtgattcca gcttcccatg gtgaaagagt cgtcttgctg
      541 actgtaatta cagtggccat cataattgat gctggtggca atatcgccga aatgagtaaa
      601 gtggattttg tgaacgctat tataaaaaga agcccccata ggcgatatgg atgaagtaca
      661 aaatagttca tcaaagcatt tatccactcc atcaatgtct ttccttctgt gaatttttct
      721 ttgctattta accgtgatac gacttaattt aggcttaaac accttacatt tacaaggtac
      781 tcctgtgggg ggccctgcca cggagcgtcg caggagtgag tgatgaggaa gaattaaagc
      841 aacctattgt gacaggcagc gataacagta aatcacagga agcataatca tggcaaaacc
      901 ggactgggag gccatcgaat cggcataccg ggccggagtc cttagcctcc gtgatatagg
      961 cgagaaatac ggcgttacag aaggggctat caggaagagg gccaaaaagc ttgattgggc
     1021 acgcagtggc ggtacgcagg tttgcaaaaa tggtacgcaa aaaaggaaag tgcgtaccag
     1081 cagaaagcct gccattactg gccttacaca aaaaagtacg caactaaaaa cagaatctac
     1141 accggatacg aaaccgatac gcggaatgcg taccgatccc ccgactaacc cattccaacc
     1201 cggtaaccag caggcattaa aacacggtgg ttatgcccgt cgccttctgc tcaaagatga
     1261 ggtgatagag gacgctaaag cgttgacgct cgaggacgaa ttatttcgcc ttcgtgctaa
     1321 caaccttgtc gctgcagaga atattggtcg gtggttggtg tcgctggaag atgctaatgg
     1381 ggaccaggaa aggaagatgc tgattgaaaa tatcagcgcc gccgagaaag caatgatgcg
     1441 caatacagtt cgtattgagt ccatcgttgg cacgcttgcg acggtaggaa aaatatttgc
     1501 tgatacagcc taccgcaagg ccgccactga taaggtgtct ctggaggctg atcgtcttcg
     1561 ccgtgatgca ggtattgatg atggcaatgg agagcgtgac ctcaatgact tctactctga
     1621 catccaaacc gacgctgaat ccggctttac gtagtttctg gactatgcgg gcacgtaaca
     1681 aagtgcttta tggtggtcgg tcatcgtcaa aatcatggga tgccgctggc attgccatat
     1741 ttctgtcgaa taaatacacc ctgcgttttt gttgtgcccg tcagatccag aataaaatcg
     1801 aagagtcggt gtataccctg ctcaaaattc agatagacag gtttggtctg cggcaccgtt
     1861 tccgtattct gaacaacaaa atcattaacc gggttactgg ctcggaattt gttttttatg
     1921 gattatggcg caacatcgaa gaaattaagt cactggaggg gatcgatatg ttgtggttgg
     1981 aagaagccca cgcactgacg gaataccagt ggaaaattct ggagccaacg atccgtaaag
     2041 agggttcgga atgctggttc atattcaacc ccggacttgt tactgatttc gtctggcgca
     2101 acttcgttgt tgatccgccc gaaggcactc tcatccgcaa aattaactat gacgaaaatc
     2161 cgtttctgtc tgacaccatg cttaaggtta tcgacgcggc gcgacgccgt gatccggatg
     2221 gttttaaaca tgtgtatgag ggcgttccgg agtctgatga tgatgcggc
//
LOCUS       DAZOZX010000299         2259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZOZX010000299 DAZOZX010000000
VERSION     DAZOZX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.305"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            130..1212
                     /locus_tag="ACHUPT_004276"
     CDS             130..1212
                     /locus_tag="ACHUPT_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR0821569.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1701..2259)
                     /locus_tag="ACHUPT_004277"
                     /pseudo
     CDS             complement(1701..2259)
                     /locus_tag="ACHUPT_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          668 a          458 c          453 g          680 t
ORIGIN      
        1 atagggatct acttcacaat ttaaatgaga aactaaaact tacatcttga aataatctca
       61 ttgattggat gaatatttgt agtgcagtga catcattttt aaataatagt tcaaataaaa
      121 gggctcacga tgaaaaaatt aacagtggca atttctgctg tagctgcatc agtactgatg
      181 gcgatgtctg ctcaggcagc agagatttac aataaagaca gtaacaagct ggatctgtac
      241 ggaaaagtta atgctaagca ctacttctct tctaacgatg cagatgatgg cgacacaact
      301 tatgttcgtc tgggcttcaa aggcgaaact caaatcaacg atcaactgac tggtttcggt
      361 cagtgggaat atgaattcaa aggcaaccgt gctgaatctc aaggttcttc caaagataaa
      421 acccgtcttg ccttcgctgg cctgaaattc ggtgactacg gctccatcga ttatggccgt
      481 aactacggtg tagcatacga tatcggtgca tggaccgacg ttctgccaga attcggtggc
      541 gatacctgga cccaaacaga tgtgttcatg actggtcgca ccacaggtgt tgcaacctat
      601 cgtaacaacg acttctttgg tctggttgat ggtctgaact ttgctgctca gtatcagggt
      661 aaaaatgacc gcactgacgt aactgaagct aacggtgatg gtttcggttt ctccactact
      721 tatgagtatg aaggattcgg tgtaggtgca acctatgcta aatctgaccg cactaatgat
      781 caggttatct acggtaacaa cagcctgaat gcatctggtc aaaatgctga agtatgggca
      841 gctggtctga aatatgatgc gaacaacatc tatctggcta ccacctattc tgaaacccag
      901 aacatgactg tttttggtaa taaccatatt gccaacaaag cacaaaactt cgaagtagtt
      961 gcacaatatc agttcgactt cggtctgcgt ccgtccgttg cttacctgca atctaaagga
     1021 aaagacttgg gtgcgtgggg tgatcaggac ctgattgaat atattgatgt aggtgcaacc
     1081 tattacttca acaaaaatat gtccactttt gttgattaca aaatcaacct gattgataag
     1141 agcgatttca cgaaagcatc tggcgttgct accgatgata tcgttgctgt aggattggtt
     1201 taccagttct aatctgatta ctcaaagata tgttgtggga ggctttgcct ccccaacata
     1261 taagtggcgc tctcaagcca ctttccttag aagcactatc ttgcttctga ctatataaat
     1321 taccctttat tttctgggac gtttcaacgc cccattttta ataatactca gtgaacaatt
     1381 aacgtatcaa ttagatttaa taacaacgat atccgtacct aacctgatat ctaatgtcat
     1441 aagcatccct ttaattatcc cctcaacctc ttgtaaattt cccactcttg agctccacat
     1501 agcccaaacg ccggatgcaa aaaaaatcat acgtgcatct ctgaaattgt gatggaggaa
     1561 gtgagtatca attatatgtg cttataaacg ttcccgattg gcacagaagt caccggattg
     1621 ttcaggctcc aataggttga gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt
     1681 tgctccttaa taaaatactt ttactctctg gacgcttttc ttagctctct tggtgttata
     1741 ttaaaacgat tatggaacct ttcagtaaaa cgagaaggac acttgtaacc attttccctg
     1801 gcaatctcgc tgatgggttt tactgtcgtt tgtatagcag gacaacgcat tatttaacct
     1861 cacgtcgtcc aatatacttt gaaaacttat tccctcgctt gccagacggc gatgcaatgt
     1921 ggaaacagaa atgtagagat atctcgctac cttgtttgct gtccattttg ttctaggttc
     1981 ggatagcatt aggttataac aacgacttat taatgattgt ttactatctg ataaaagtaa
     2041 acaattaaca tgatgtgctc ctaatgaaag tagaacgccc attgctaagt gttcctgaat
     2101 ttgcgttgag aagcctcggg aaatagatgt ttttagttgc tcccaacaat atattaactc
     2161 ggggttctga ggtaagcaga aacctgtcgt gttacgaatt tgatcagtta tcgcataaaa
     2221 tttttgaaac ttttcaatta gatcaatggg ataataaag
//
LOCUS       DAZOZX010000300         2254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZOZX010000300 DAZOZX010000000
VERSION     DAZOZX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.306"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            147..908
                     /locus_tag="ACHUPT_004278"
     CDS             147..908
                     /locus_tag="ACHUPT_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0821570.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            982..2254
                     /locus_tag="ACHUPT_004279"
                     /pseudo
     CDS             982..2254
                     /locus_tag="ACHUPT_004279"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          664 a          401 c          541 g          648 t
ORIGIN      
        1 aataacaaaa ttaagaaaag ccatatttac gatatgggca acataaatat aatctaaaaa
       61 agagtaaggc gttgtaggtt ggttccgcgt tcattacctt gttagtgaac gcggatttat
      121 cagttgtcga aatggttttt ataataatga aaaattttaa tgtactaacc aaagtcgtat
      181 tttgcttgtc gtttttatca agcgcatttc ccacctatgc tgcggatgat gtcaaagtga
      241 ctcaaaacac ttcagttttt aaactgaagc ttagtgcaac gcgattaatc tatgaacaag
      301 ggtcggatgg cgcagcgata actgtgacaa atccgcagga ttggccgatg cttgtgcaga
      361 gtaaagtctt tgaagaagat aaagtaactg cagcgccctt tattgtcact ccgccactgt
      421 tcaggcttaa tccaggccag caaaataaaa tgtgggttgt gcagactgcg ccagctttac
      481 aagacaggga aaaattacac tggctatgcg ccaccggcat tacgcctaaa aagagtgatg
      541 tttgggccga gaaagataaa gaaaaaattg ctgaaaatgt gaacctgaac atccaggtta
      601 acgtacatac ctgcataaaa cttattacca ggccatctgg tattgaaggc aagatggtta
      661 atgcgagtga gaagttatcg tggaaaaagc agggtgataa aattgtcgcg gtcaatcagt
      721 cgccgttcta catcaacctt tcttctttac aggttggcgg tagcaagatt gagaaactag
      781 attatattgc gccgttttcg caacatgagt ttgttatgcc aaaaactagc ggccaggata
      841 ttaaatggtc tgtgattact gatgaaggtg gtgagagtcg tttgtttata tctcatttgc
      901 aatcataatg tattacattt ttattgaata tttttatgcc atgcatattc catgttttat
      961 cgactgtcat taattttgat aatggcctta cttgcgggtc agttaagtgc ccaggagtgg
     1021 tcatttgatt cttcacagtt ggaaggaaac gttagtgcgg acactgtggc aatgtttaat
     1081 cagggagagc aactccccgg aaattatcgt gtggaaattt atcttaacgg tgaaaaagtt
     1141 gatgttgggg agtttccctt tcatcgtccg gaatcccctg aagaaaaaga gcttgttcca
     1201 tgtttaaccg ttgatgacct tatccactat ggcattaaaa ttgataaaag cagcagtgat
     1261 actgacaata aaaagaatca gtgttttaaa tggaactcta ttgaaggttt gaaagtaaat
     1321 tatgattttg atagccagcg cgtacagata accgttccac aactgtatct gcaagataaa
     1381 aaaagtagtt tagcaccagt gtcgttatgg aatgagggtg tagctgcatt ccgcatggtg
     1441 tatcagacaa atattgatat ttccaaacaa aatgataatc aatctaccac gcgtaattct
     1501 cgttatggca ggttcactcc agggtttaat cttggtgcct ggaggtttcg tagttcagtg
     1561 acatggagca aggaacttgg ccagtcagaa agatggcaaa ggggttacat gtggtttgag
     1621 cgaggcataa atgcgattaa aagccgatta acgcttggag aatcttatac gtcgtctgag
     1681 gtgtttgaca gcataccttt tcgcggcggg atgttagcga ccgacgatgc gatgactccg
     1741 ccggaggaca gctactacac acctgtggtg catggaatag cacaaagtga ggcgcaggtt
     1801 attattaaac aaaatgggca aattattttt acccgttctg tgccgccagg gccttttgcg
     1861 ctggataatt taccaacgct ggccgttggc ggggagcttg atgttacagt gagagagagt
     1921 aatggcgagg agcagtattt ttccgtgcca tttcaaacgc cggccatcgc tctgcatgaa
     1981 ggatatttca aatatagtgt gatggggggg aatataaaga agaaggtata aaaagctccc
     2041 ctgtctccgt gggcgaaatc acggcgatgt acgggcttcc ttatggcgtc acggtgtatg
     2101 gagggattca aaacgccact cattttaatg caatatctac gggtattggc atttctctgg
     2161 ggctgttagg gagtttatcg acagatatta cgcgttcgat tgcgaattta tattatggga
     2221 ataaatatcg tattcgttat agtaaatcca ttag
//
LOCUS       DAZOZX010000301         2250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZOZX010000301 DAZOZX010000000
VERSION     DAZOZX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.307"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUPT_004280"
     CDS             complement(<1..242)
                     /locus_tag="ACHUPT_004280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821571.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHARPELRA"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="ACHUPT_004281"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="ACHUPT_004281"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0821572.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          553 c          666 g          457 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DAZOZX010000302         2240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZOZX010000302 DAZOZX010000000
VERSION     DAZOZX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.308"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUPT_004282"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUPT_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR0821573.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHUPT_004283"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHUPT_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0821574.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..1969
                     /locus_tag="ACHUPT_004284"
                     /pseudo
     CDS             740..1969
                     /locus_tag="ACHUPT_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1973..2132
                     /locus_tag="ACHUPT_004285"
                     /pseudo
     CDS             1973..2132
                     /locus_tag="ACHUPT_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          545 a          596 c          622 g          477 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgctgt
     1441 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
     1501 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
     1561 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa tgcctccggc
     1621 ggtctggttc gcgtactcac cggaccggaa aggtatccat ccacagaatc atctggccgg
     1681 ttacagcgga gtgcttcagg ccgatgctta cggtggttac cgggcgttgt acgaatccgg
     1741 cagaataacg gaagccgcat gtatggctca tgtccggaga aaaatccacg atgtgcatgc
     1801 aagagtgcca accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat
     1861 cgaggcagaa gtccggggat gttcagcaga acagcgtctg gcggcaagaa aagccagagc
     1921 cgcgccactg atgcagtcac tgtatgactg gatacagcaa cagatgaaaa ttcattcgct
     1981 gaaaatggaa tgtctccacg gagaacacta ttatccatcg ggaaatagtg cgggcaacag
     2041 tgtttagtta tatcgagtgc gattacaatc gctggcgacg gcaccgttgg tgtggtggtc
     2101 tcagtccgga acaatttgaa aaccagagcc tcacttacga ctgtgtccat attatgtggg
     2161 taggatcaat aaagacgttt tatatgtgtc tgcttgcagc cagaaaattt ataccctgca
     2221 ggtatcataa acatgcttga
//
LOCUS       DAZOZX010000303         2239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZOZX010000303 DAZOZX010000000
VERSION     DAZOZX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.309"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(121..546)
                     /gene="arsC"
                     /locus_tag="ACHUPT_004286"
     CDS             complement(121..546)
                     /gene="arsC"
                     /locus_tag="ACHUPT_004286"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR0821575.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(559..1848)
                     /gene="arsB"
                     /locus_tag="ACHUPT_004287"
     CDS             complement(559..1848)
                     /gene="arsB"
                     /locus_tag="ACHUPT_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0821576.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1902..2237)
                     /gene="arsR"
                     /locus_tag="ACHUPT_004288"
     CDS             complement(1902..2237)
                     /gene="arsR"
                     /locus_tag="ACHUPT_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0821577.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          595 a          615 c          560 g          469 t
ORIGIN      
        1 agtaagttgg cagcattacc tgaatttgca cggttttgta ggtcggataa gacgcgttag
       61 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      121 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      181 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      241 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      301 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      361 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      421 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      481 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      541 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      601 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      661 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
      721 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
      781 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      841 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      901 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      961 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
     1021 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1081 tgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
     1141 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1201 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1261 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1321 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     1381 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1441 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1501 aagcgccgcg ccatcattgg caaacagggc cgcaacggcg gcaccgagca ggacaatcca
     1561 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
     1621 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     1681 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
     1741 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1801 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     1861 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
     1921 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
     1981 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
     2041 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2101 gccagatggc gggatatctt gggctgcgac tgatcgaggg cagtgcagag atcgcagacg
     2161 cataactctc ccagttcgct aagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2221 attttgaaca attggatgg
//
LOCUS       DAZOZX010000304         2231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZOZX010000304 DAZOZX010000000
VERSION     DAZOZX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.310"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            2..955
                     /gene="lsrR"
                     /locus_tag="ACHUPT_004289"
     CDS             2..955
                     /gene="lsrR"
                     /locus_tag="ACHUPT_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR0821578.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            1034..1861
                     /locus_tag="ACHUPT_004290"
                     /pseudo
     CDS             1034..1861
                     /locus_tag="ACHUPT_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
BASE COUNT          542 a          525 c          617 g          547 t
ORIGIN      
        1 tatgacaatc aacgattcgg caatttcaga acagggaatg tgtgaagaag aacaggtcgc
       61 gcggatcgcg tggttttact atcacgacgg gctgacccag agcgagatca gcgatcgtct
      121 cggcctgacc cgtttgaaag tgtcgcgctt gctggagaaa gggcatcagt ccggcattat
      181 tcgcgtacag attaattctc gctttgaagg ctgtctggaa tatgaaactc aattacgtcg
      241 tcagttttcg ctgcaacatg tccgggtgat ccctgggctt gcggatgctg atgtcggcgg
      301 gcgactaggg ataggcgcgg cgcatatgtt gatgagttta cttcaaccac aacagatgct
      361 ggcgattggt tttggcgagg caaccatgaa tacgctgcaa cgcttaagtg gttttatttc
      421 gtcacagcaa attcgcctgg tcacgctctc cggtggcgtc ggttcttata tgacgggtat
      481 cgggcagctt aacgcggcgt gcagtgtgaa tattattccg gctccgttgc gggcatcctc
      541 cgctgacatt gcccgtacgc taaaaaatga aaattgcgtc aaagatgttc tgctagccgc
      601 gcaagcagcg gatgtggcga ttgtcggcat tggagccgtg agtcaacaag acgacgcgac
      661 aatcattcgc tccggttata tcagccaggg cgaacagtta atgattggcc gaaaaggggc
      721 ggttggcgac attttaggct acttttttga tgcaaaaggt gacgttgtca cggatatcaa
      781 aatacataac gaactgattg gcttaccttt aagcgcgctg aagaccatac ccgtccgggt
      841 tggcgtggca gggggagaaa ataaagctga agcaattgcc gctgcaatga aaggcggtta
      901 tatcaacgca ctggttaccg atcaggacac agcagcggcg attttacgta gttaaatttg
      961 catgacctta ttaccgggaa tgatggagaa cgtatagagg aacagacaga acgcataagc
     1021 cgaggataat ctaatggctc gactctttac cccttcagaa tcaaagtact acctgatggc
     1081 gctggatgca ggcaccggaa gtattcgggc tgtgatattc gacctggaag gcaatcaaat
     1141 agcagtggga caggcggagt ggcggcatct ggcagtaccg gacgttcctg gttctatgga
     1201 atttgatctc aacaaaaact ggcaactggc gtgtgagtgt atgcgccagg cgctgcacaa
     1261 cgccggcata gacccggagt atatcgctgc cgtttcggca tgttctatgc gtgaaggcat
     1321 tgttttatat aacaatgaag gaacaccgat ctgggcctgc gccaatgtgg atgccagagc
     1381 ggcacgcgaa gttagcgaac ttaaagaact gcacaacaat acctttgaaa acgaagttta
     1441 tcgagcgacc ggacaaacac tggctttaag tgccatcccc agattacttt ggctggcgca
     1501 ccatcgttcc gatatttacc gtcaggcatc aaccatcacc atgatcagcg actggctggc
     1561 ctatatgctc agcggcgaac tggcggtgga tccctctaac gctggcacca cgggacttct
     1621 tgatctaacc acccgtgcct ggaaacctgc attgctggat atggctggcc tgcgtgccga
     1681 tattctttct cctgtcaaag aaaccggcac attgctgggc gtggtaagtt cacaagcggc
     1741 ggaactctgc ggtctgaagg cgggcactct ggtggtcgtt ggaggaggcg acgtgcagct
     1801 tggttgcctt gggttaggcg ttgtgcgtcc ggcacaaacc gcggttcttg gcggcacgtt
     1861 cacggataat aagcagtgca cgctcagcga ggcccggcat cagttgccga ttactccgtt
     1921 ccgtatgccg tcatgagtcc catcctttga aagcagtgat cgctgtggtg agactgtagc
     1981 gcggacatgt ctatttagtc agagacggac atctcaattt agctactaca atattactac
     2041 gcataatgta tattatgtta aatcatgtct gagtgatgcg aagtgctttt ctggtagtcg
     2101 ttattcgttc agggttcaag aacttaagcc tctcctcttt cataattggc ctgtgcatgg
     2161 agaaatagcc tggttattcc ctgccccatg ctaagagcgc agtgtctgtc gcatagacac
     2221 tgcatacgaa a
//
LOCUS       DAZOZX010000305         2208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZOZX010000305 DAZOZX010000000
VERSION     DAZOZX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.311"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            376..1566
                     /locus_tag="ACHUPT_004291"
     CDS             376..1566
                     /locus_tag="ACHUPT_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR0821579.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(1956..>2208)
                     /locus_tag="ACHUPT_004292"
     CDS             complement(1956..>2208)
                     /locus_tag="ACHUPT_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR0821580.1"
                     /translation="SMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEK
                     IYPIVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT          620 a          455 c          484 g          649 t
ORIGIN      
        1 ttaatcaacg agttcaatat taatttatta tttttcaaag tcgcactata taggcattta
       61 cttccgcagt accaccgata gtatcgatat caaatcagcc tgcggccgcg ataactttcg
      121 taacattctg ccagcggctt tgttgaataa atcaggttca agataatcgt ctctgcatca
      181 ggttagagtt tcaggcaata catcctcttt ctggcatacc tgccactctc gcaccatggt
      241 catagccttt acgatctgca tactcaccag accagagccc gctccctttt aggctgagga
      301 atccccagaa aagaagacag gcatagagtt ttgtgatcta ctgattgcgc taacaaattc
      361 aatgagatca cctctatgcc tgcccgtaaa gtatgccaga cttttttccg taatgcttta
      421 gcttccacgc atcagtatcg acaaaatgcc attattgatt ctgctgctgc tttagtcggt
      481 ggcgcatctc tttctcttac cagcattggc cggcatttac ccggtcctgc tcgcgtgaaa
      541 gataaaataa aacgcgttga ctgtctgttg ggtaataaac gacttcataa tgatatccct
      601 ctgatattca aaaatattac atccatgatg acaaataaac tgtcgtggtg tgttatcgct
      661 gttgactgga gcggttaccc ttttcaggaa tatcatgtcc ttcgtgccag cctcctctgc
      721 gacggtcgct ctatccccct gatgagtcag gtcttcccgt caaaaaaaaa taacgaggcg
      781 gtggaaatcg cttttctgga tgcgctttcc ggggccattt ctccccggac tcgtgttgtt
      841 attgttactg atgctggttt tcaaagtgca tggttccgtc atatcaaatc acagggatgg
      901 gattttattg gtcgtatcag gggggtagtg aagttcaggc tggatagtga caaagataag
      961 tggctggaca taaaaatgtg cagggggagt tcagaggcaa aatacctggg gacgggaacc
     1021 ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt atctttataa acattcacct
     1081 aaaggcagaa aaagcaggcg tgccagaggg aggccgggct taccaacaac ggaaaaagaa
     1141 caaaaagccg caggcagaga accatggtta atattcagca atacagctga gtttaatgcc
     1201 aaaaaaatca tgaaactgta cagtcgaaga atgcagatag agcaaaactt tcgggatgaa
     1261 aaaagtgaac gctttggttt tggccttcgg gcgagcagaa gtcggagggg agagcgtttt
     1321 ctggtattaa gtctactggt tacactggcc agtatcgtac tctggctact tggttatcat
     1381 tttgaaaata aaggatttca tttaaagtat caggctaata gtctcaaaaa acgaagagta
     1441 ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta accctgaact tctacgacga
     1501 gcacaacctg aaaagatatt gtgccggttg gccagcactt accgaagtat ggtgctggct
     1561 tactgatgaa aattttctgg ggatccctca gcctttcggg gaggaatgag ggctctgata
     1621 ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat ttggcctgag tgacccatgc
     1681 ctcccaaaag cagagtcgct agtgttatgt aattaggcgg ttaaacttca attctcagtg
     1741 ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt tttaattgac taatattaga
     1801 aacggttagt gtgattgtat atagatattt gagtgtaagg tatttaaagg ggcattcagc
     1861 ccctccgtct atttgtgatt gcatccattg atgtgtgtat tattttgagc ttgaaaaagg
     1921 gaggcgctct ttaataataa ataaatctat caaagctaaa tattaaatgg aagccaccct
     1981 tttttctctg atagttttat tatcaccaaa ccatttattt cagctatcga atctggattc
     2041 ggccaggaag gtaaactctt cacaattgga taaatcttct ctatttgttc tttatttgct
     2101 ggtttgcaat tacagattcc atagtaattc ataaatgcaa ccatcctaaa atagttgcca
     2161 ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc tcatggag
//
LOCUS       DAZOZX010000306         2184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZOZX010000306 DAZOZX010000000
VERSION     DAZOZX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.312"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="ACHUPT_004293"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="ACHUPT_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0821581.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="ACHUPT_004294"
     CDS             578..1027
                     /locus_tag="ACHUPT_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0821582.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /gene="ydcY"
                     /locus_tag="ACHUPT_004295"
     CDS             complement(1028..1261)
                     /gene="ydcY"
                     /locus_tag="ACHUPT_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0821583.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="ACHUPT_004296"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="ACHUPT_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0821584.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="ACHUPT_004297"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="ACHUPT_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0821585.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          535 a          493 c          530 g          626 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atct
//
LOCUS       DAZOZX010000307         2183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZOZX010000307 DAZOZX010000000
VERSION     DAZOZX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.313"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUPT_004298"
     CDS             <1..203
                     /locus_tag="ACHUPT_004298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821586.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHUPT_004299"
     CDS             293..694
                     /locus_tag="ACHUPT_004299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821587.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHUPT_004300"
     CDS             696..1301
                     /locus_tag="ACHUPT_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR0821588.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHUPT_004301"
     CDS             1288..>2183
                     /locus_tag="ACHUPT_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0821589.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZOZX010000308         2181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZOZX010000308 DAZOZX010000000
VERSION     DAZOZX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.314"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="ACHUPT_004302"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="ACHUPT_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /gene="yniD"
                     /locus_tag="ACHUPT_004303"
     CDS             1128..1235
                     /gene="yniD"
                     /locus_tag="ACHUPT_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0821590.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="ACHUPT_004304"
     CDS             complement(1288..2046)
                     /locus_tag="ACHUPT_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0821591.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          655 a          484 c          455 g          587 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt c
//
LOCUS       DAZOZX010000309         2176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZOZX010000309 DAZOZX010000000
VERSION     DAZOZX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.315"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..495)
                     /locus_tag="ACHUPT_004305"
     CDS             complement(<1..495)
                     /locus_tag="ACHUPT_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0821592.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNY"
     gene            809..>2176
                     /gene="icsB"
                     /locus_tag="ACHUPT_004306"
     CDS             809..>2176
                     /gene="icsB"
                     /locus_tag="ACHUPT_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR0821593.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSI"
BASE COUNT          762 a          421 c          322 g          671 t
ORIGIN      
        1 ataatttttc ccatgatgac taatattctt gtcgaccggt ccccaattct tattatttaa
       61 ataatcagta aatatttgtt ttattttcgt ttgcaattcc tttttatttt taatgcctag
      121 ctgtgctctt atctcttcac ttattacttt attaagctca ttaatatgtc ttttattaac
      181 ttcaccaagt ttataggtat ctaatcgttc catcatggct ttaatttttg acgtatcacc
      241 acctgccaac tttattgcct cgcaaaaaac ttcagctctg tcacgcgcaa acaaaaggct
      301 ttttcctgtt aagcttgttt ggttctgtaa atataatcga ttaagcgctt cgatcccctt
      361 atttctttct ccatttttaa ctctttgata tgaaatcacg cttacacctt tgtgctttcc
      421 ggtttcttct gcgcctttat aggaatctcc gctttgaaat gaaacctgat gcaatcccaa
      481 attagttatg tgcatttaat tatcctcagt cataaatggg gcattgattg cagttttgta
      541 cctcgtttta caacaaaaaa gatacttatt caacagggtt ctctcttttt ctataacact
      601 ccgagcctga tacatcaggc actttatctt gtgggatttc atgatgaaac gagcactaca
      661 tatgctcacg aggtacatat tatgtacagc aaaagaaaat ttgattatgt taacaggctc
      721 aagtttctaa atttattaat tgaatatatt atataccaaa tacattttac atccccacaa
      781 tcaccaagta atggagagtt aataaagtat gatcctcaaa attagcaatt tcattgacgc
      841 aagcaataca aaagggccta tacgcgttga agatacagag catggaccta tattgatagc
      901 acagaaattc aacctaaagg atctcttttt cagaacatta agcactatta atgcaaagat
      961 aaattcacag atattaaatg aacaactaaa aaattatcga ctagagaatc aaaaatcctt
     1021 attgcttttc ctcaatacat tagctagcga aaaatctgca gaaagtgcgt ttgccgccta
     1081 tgaagcagct aaaaactcaa ttcaacactc tttcacaggt agagacatca aattaatgtt
     1141 gaataccgca gagcgttttc atggcatcgg tacagccaaa aatcttgaaa gacatttagt
     1201 ttttcgttgc tggggaaata gaggcataac ccatttaggg catactagta tcagtataaa
     1261 aaacaattta ctacaggaac caactcatac ctatctcagc tggtatcccg gcgggaatgt
     1321 tacaaaagat acagaaataa actacctttt tgaaaaacgc tcaggttaca gtgtcgatac
     1381 ctataagcaa gacaaactaa atatgatttc agaccaaaca gccgaaagac ttgatgcagg
     1441 ccaagaggtt agaaacttat taaattcaaa acaagatcaa aataacaaca aaaaaatatt
     1501 ttttcccaga gctaatcaaa aaaaagaccc ctatggttat tggggggtca gtgctgataa
     1561 agtatacatt ccattatcag gtgataataa aacaaaggat ggtaaaatca gccataacct
     1621 ttttggcctt gatgaaacaa acatgtcaaa atttatatgc aaaaagaaag cagatgcttt
     1681 cagacaattg gcaaattata aattaatcag taaatctgaa aactgtgctg gtatggcact
     1741 taatgttctt aaagcaggaa attctgaaat atactttcca ctccctgacg ttaaattggt
     1801 tgctacacct aacgatgtat acgcgtatgc aaataaagtt aggcaacgca ttgaaagtct
     1861 caatcagtct tataacgaaa taatgaagta tattgaatct gattttgatc tttccagatt
     1921 gactcaatta cgacgcagct atcttaaaag tttcaataaa attaacctta tccacacacc
     1981 aaaaacattc aagcctttat caatttcact ttacaaacat cctactgaaa atgtatcttc
     2041 agaagacttt gatgcggtca tcaacgcttg ccactcatac ttagtaaaat cagcacccag
     2101 caatatgact cgtgtattaa acgaattaaa aaccgaagca acagataaaa aagaagaaat
     2161 catcgaaaaa tcaata
//
LOCUS       DAZOZX010000310         2164 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZOZX010000310 DAZOZX010000000
VERSION     DAZOZX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.316"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHUPT_004307"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHUPT_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR0821594.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHUPT_004308"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHUPT_004308"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR0821595.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..>2164
                     /locus_tag="ACHUPT_004309"
     CDS             1849..>2164
                     /locus_tag="ACHUPT_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0821596.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYI"
BASE COUNT          587 a          482 c          511 g          584 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atct
//
LOCUS       DAZOZX010000311         2134 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZOZX010000311 DAZOZX010000000
VERSION     DAZOZX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.317"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHUPT_004310"
     CDS             complement(<1..104)
                     /locus_tag="ACHUPT_004310"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR0821597.1"
                     /translation="MCHPQPKPPAPPAWAMMPPSNSLQLLDETFSVSGT"
     gene            complement(64..399)
                     /locus_tag="ACHUPT_004311"
                     /pseudo
     CDS             complement(64..399)
                     /locus_tag="ACHUPT_004311"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(396..929)
                     /locus_tag="ACHUPT_004312"
     CDS             complement(396..929)
                     /locus_tag="ACHUPT_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0821598.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(995..1624)
                     /locus_tag="ACHUPT_004313"
     CDS             complement(995..1624)
                     /locus_tag="ACHUPT_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821599.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1628..1843)
                     /locus_tag="ACHUPT_004314"
     CDS             complement(1628..1843)
                     /locus_tag="ACHUPT_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0821600.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          546 a          567 c          471 g          550 t
ORIGIN      
        1 gtcccggata ccgagaacgt ttcgtccagc aactggagag agttcgacgg tggcatcatt
       61 gcccatgccg gaggcgctgg aggtttcggc tgaggatggc acagggcatt ttcctttgac
      121 gagcacccga ccaccattat caagcttgcg ccgaagagca tcattttcag ctttcgcatc
      181 agctaactcc ttcgtgtatt tagcatcgag tgcatcagca tcacgctggc gctgctgcat
      241 gtcagtaatg gttgcgttcg ccagcttcag ttctccggcg tttttgtcgc gctgctcttt
      301 gtaggtaatg gcgttatcac ggtaatgatt aacagcccat gacaggcaga cgatgatgca
      361 gataaccaga gcggagataa tcgcggtgac tctgctcatt gatctatccc ccaacaggct
      421 aatgcgcttt cctggtcacg acgaataacc tgtccatagc agttatttga acgtatgcgg
      481 caatcgcgcc caacatcttt tatccaccag cgaatcgcct cgcatgcgcc cttacgatca
      541 ccggcattca gccgcttata aaacgtcgac gggaaacact taccggggcc aatgttatag
      601 gggcaaaatg acgctatacc cgctttctgg ggttcggtca gaggtacttt aatattgcgc
      661 tccacccatg ccagcgcctt atcacgttca atagcgttaa cctggtcaca tttttccttc
      721 gacagcttca ttcccggtat gacaggctta ccatccacca ttgtggcacc acgacagatg
      781 gtccatatgc cagaaccatc acggtatgcc gttgtgtgat taccctcttt ttcgttcaga
      841 aactggtcaa gtatctgggg agcagacgcg cctgcggcaa tcagcgccag aacagcagcc
      901 gacaggccgt atttgatttt tgcgctcatg gatatttatc aggatttatc ggcaacaaat
      961 aacgagccaa cttatatacg ccttttaaga taagtcagcc ccggctggat ccagtcatca
     1021 ggctctttct taaaggggga gtattgaaat cacgaagaag agcctcccgc accgcagcat
     1081 ccatgtctgc accactggca agtgcttcaa tctctgccgc cacctgcaaa taccccatgc
     1141 aacggccaat gcgtttcata actctggtgt tttcgtcttt caggcatctg atagcgagat
     1201 taataatttt caggcagtga tgatgcgatg cccggacagg ttttccgttc tcataaacga
     1261 cgaactcacc ctcttcacag gaaaccctga aaggccgatg gggttcctgg tctttatccg
     1321 gtgcagacgc aataaaatca cttttatgct cttcctctcc ggcactgtta accgtaatga
     1381 cgtactcagg ccgcaggtaa gccgcctctt tttctgacaa tgcggccggt aaaaactttt
     1441 cctgcaaaaa cttcataaaa gtctcgtgag cgactttgtg gcagtaatcg taaatcgccg
     1501 cataatgctc atcgcggcgt ttgttttcat cttcagaact caccaacgcc gacaattttt
     1561 tattaagctc agtgatttca ttttccaggt gattgaatcg ctgattcatt tcttcatgct
     1621 tcattattta ctctccccgt gcagccttac gcttgtcttc tcttattttg aaatacagat
     1681 tcgtcagata agtcagaagc cccagaagca gacttcccag cacaccaatc gcagcccact
     1741 gtgatggcct gacctgatcc aaccactgca aaaaccagta gccggcactg ccggcggagg
     1801 tgccgtaggc aatgcccgtt gaaattttgt ccatggattt catagcctca cctccgcaaa
     1861 taacggatgg cgtagtttta cactgagaaa tgaaagggat ttgaaaagaa aaaaacgcaa
     1921 aagcgggcga aacgatatat acagtaagga aagcactcta tccaacaaac cacccacagt
     1981 taatcggaat aaaagcagag tgcttatgaa tgatcgcacg cccgaaggtt agtatttctg
     2041 cacagcaatt ttgcaaaaaa aggcgatcat tcataactta aacgtctttc agtcactccg
     2101 ggatttccca tcatcgcaga ctgaaagact ctga
//
LOCUS       DAZOZX010000312         2132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZOZX010000312 DAZOZX010000000
VERSION     DAZOZX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.318"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..132
                     /locus_tag="ACHUPT_004315"
     CDS             <1..132
                     /locus_tag="ACHUPT_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0821601.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACHUPT_004316"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACHUPT_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACHUPT_004317"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACHUPT_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            977..1555
                     /locus_tag="ACHUPT_004318"
     CDS             977..1555
                     /locus_tag="ACHUPT_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821602.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1664..1922)
                     /locus_tag="ACHUPT_004319"
                     /pseudo
     CDS             complement(1664..1922)
                     /locus_tag="ACHUPT_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1906..2132
                     /locus_tag="ACHUPT_004320"
                     /pseudo
     CDS             1906..2132
                     /locus_tag="ACHUPT_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          670 a          378 c          522 g          562 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat
     1021 atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc
     1081 aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga
     1141 caatgagata acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc
     1201 cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat
     1261 tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac
     1321 tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat
     1381 tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg agtctactgg
     1441 cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga
     1501 aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg tttaatttta
     1561 gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc
     1621 tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga
     1681 ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg
     1741 ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct
     1801 gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct
     1861 caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc
     1921 atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa
     1981 ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt
     2041 cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa
     2101 tccaggcagg aaaagctgat atggctgcgt gc
//
LOCUS       DAZOZX010000313         2118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZOZX010000313 DAZOZX010000000
VERSION     DAZOZX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.319"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            37..498
                     /gene="hycA"
                     /locus_tag="ACHUPT_004321"
     CDS             37..498
                     /gene="hycA"
                     /locus_tag="ACHUPT_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0821603.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            623..1234
                     /gene="hycB"
                     /locus_tag="ACHUPT_004322"
     CDS             623..1234
                     /gene="hycB"
                     /locus_tag="ACHUPT_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0821604.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            1231..2118
                     /locus_tag="ACHUPT_004323"
                     /pseudo
     CDS             1231..2118
                     /locus_tag="ACHUPT_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT          440 a          564 c          606 g          508 t
ORIGIN      
        1 taaagctggc atctctgtta aacgagtaac ctgacaatga ctatttggga aataagcgag
       61 aaagccgatt acatcgcaca gcggcatcgt cgcctacagg accagtggca catctactgc
      121 aattcgctgg ttcaggggat cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc
      181 gcgccggaca aagaactctg tttcgtcctt tttgaacatt ttcgtattta cgtcaccctg
      241 gcggatggct ttaacagcca caccatcgag tattacgtcg aaacaaaaga tggcgaagac
      301 aaacagcgga ttgcgcaggc gcaactgagc attgacggca tgattgatgg caaggtcaac
      361 atccgcgatc gcgaacaggt tctggaacac tatctcgaaa aaatcgctgg cgtttacgac
      421 agcttataca ccgccattga aaacaatgtg ccggtgaatt taagccaact ggtaaaggga
      481 caaagcccgg cagcatgagc tgaggctttg cccgttttgc aggcgttacg cctgtttggg
      541 gatgggcgtg tcgatgagtg tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg
      601 acaatcacct gaggaatgcc tggtgaatcg ttttgtaatt gccgactcca cgctctgtat
      661 cggctgccac acctgtgagg ccgcctgttc agagacgcat cgccagcacg gcctgcaatc
      721 aatgccgcgc ctgagagtga tgctcaatga aaaagaatct gcgccgcagc tctgtcacca
      781 ctgtgaagat gcaccctgcg cggtggtctg cccggttaac gccatcaccc gcgttgatgg
      841 ggccgtgcag ttgaatgaaa gtctgtgcgt aagctgcaaa ctgtgcggca tcgcctgccc
      901 gtttggcgca attgaatttt ccggcagccg tccgctggat attccggcaa acgccaatac
      961 cccgaaagcg ccaccggcac cgcctgctcc ggcgcgtgtc agcacattgc ttgactgggt
     1021 gccaggtatt cgcgcgatcg ccgtcaaatg tgacctttgt agctttgatg aacaaggtcc
     1081 ggcctgtgtg cggatgtgcc cgactaaagc cctgcatctg gtggataaca ccgatatcgc
     1141 ccgcgtcagc aaacgtaagc gtgagctgac ctttaacacg gactttggcg atctcacctt
     1201 gtttcagcag gctcaaagtg gagaggctaa atgagcgcaa tttccctgat caatagcggc
     1261 gtggcatggt ttgtcgccgc cgctgttctg gcatttctct tttcaaaaag cgctaagtgg
     1321 ctggatagcc ggaattggcg gcgcggttgg tagtctgtat acggcagccg cgggcttcac
     1381 tgtactgact ggcgcggttg gcgtgagcgg tgcgctgtcg ctggtaagct acgatgtgca
     1441 aatctccccg cttaacgcga tttggctgat taccctcggc ctttgcggtc tgtttgtcag
     1501 cctctataac attgactggc atcgccacgc gcaggtgaag tgcaacggct tgcagatcaa
     1561 tatgttgatg gctgccgccg tctgcgccgt catcgccagc aacctcggca tgttcgtggt
     1621 aatggccgaa gtcatggcct tgtgtgcggt gttcctcacc agcaacagca aagagggcaa
     1681 actgtggttt gcgctgaggc gtcttggcac tctgctgctg gtgattgctt gctggctgct
     1741 gtggcagcgt tatggcacgc tggatctgcg cctgctggat atgcgtatgc aacagctgcc
     1801 gctcggttcc gatatctggc tgctcggtgt gattggcttt ggcctgctgg ctgggattat
     1861 tccgctgcac ggctgggtgc cgcaggcaca tgctaacgcc tctgcgccag ctgccgcgtt
     1921 gttttctacg gtagtcatga aaattggcct gctgggcatg ttaaccctgt cactgctggg
     1981 cggtaatgca ccgctgtggt gggggatcgc gctgctggtg ctcggcatga tcaccgcgtt
     2041 cgtcggtggt ctgtatgcgt tgatggaaca caacatccag cgcctgctgg cttaccacac
     2101 cctggaaaat atcggcat
//
LOCUS       DAZOZX010000314         2094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZOZX010000314 DAZOZX010000000
VERSION     DAZOZX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.320"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..581)
                     /gene="spa29"
                     /locus_tag="ACHUPT_004324"
                     /gene_synonym="spaR"
     CDS             complement(<1..581)
                     /gene="spa29"
                     /locus_tag="ACHUPT_004324"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR0821605.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVL"
     gene            complement(581..841)
                     /gene="spaQ"
                     /locus_tag="ACHUPT_004325"
     CDS             complement(581..841)
                     /gene="spaQ"
                     /locus_tag="ACHUPT_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR0821606.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(856..1506)
                     /gene="spa24"
                     /locus_tag="ACHUPT_004326"
                     /gene_synonym="spaP"
     CDS             complement(856..1506)
                     /gene="spa24"
                     /locus_tag="ACHUPT_004326"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR0821607.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            complement(1511..>2094)
                     /gene="spa33"
                     /locus_tag="ACHUPT_004327"
                     /gene_synonym="spaO"
     CDS             complement(1511..>2094)
                     /gene="spa33"
                     /locus_tag="ACHUPT_004327"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR0821608.1"
                     /translation="TFRITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSL
                     HHLLEFCLGSSNVRLATLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINL
                     SESNGESEHTEVSLALFNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKH
                     VNIKVNGSLVGHGELVSIEDGYGIEISSWMVKE"
BASE COUNT          827 a          396 c          309 g          562 t
ORIGIN      
        1 aacaatacta tcataacagg actggctaaa ataacagcct gacttgccaa taaagtcaga
       61 aatgttaaga tatttgagac acggaaagaa cattgagaaa ataacgggca tatattataa
      121 gacaattgta tggattctaa aataaagacc ataccaccac tgtatagaaa tacaactgca
      181 gaaaaaagat tgaaaaattt tgccaactca gacgtatcaa caccattggc aggatcaatt
      241 gaactactaa gcgttgcccc acgctggttg tcaataatgc taccaacagc atgaaatatc
      301 caaaagggaa gagacaagca aaaagagaga aggaggccaa caattatttc cttgaacata
      361 aggaaataaa catgttcaaa aacagaagaa cctatgtcta ccttaccaga agtaattaat
      421 cctgatgcaa caagaaaaat cacaggtatt ctaatagatg gagaaattat accgttattt
      481 aaaaatggaa gaaagaaaaa taatggagcc aatctaaaaa aaacgccgtt caggagtatt
      541 aaaaacacat gaatactttc gaaccagctt gaaatgtcca tcaaacccca ctcttaatta
      601 aaaacattat ttcatgacaa aaagacaata aaacctcacc ataccatcca gaaagaagta
      661 gcaaagatat tgagacacct ataagcttta taccaaaagg aagtgtctgc tcttggagtt
      721 gagtcactgt ctgtaacaaa ccaatagata atccaataac cgtagctata cctactggcc
      781 ataaggaaaa gataaggatt aaataaagag ccttattacc catataaact atatcagaca
      841 cattacctcc atcatctaag caggaatatt gatatattgt tcaatcaagg ccttagacag
      901 gataccccac ccatcaagcg caacaaataa tacaagtttt attggtactg atattgtgat
      961 tggactcatc atcatcatac caagtgcaag taaaatgctt gaaataacaa gatcaacaac
     1021 aacaaatggc aaatataaat aaaaacctat tttgaacgca tctttgattt cacttagcgc
     1081 atatgcaggc aagagggaaa acaatgaata atcattattc tctgcactct ttaaatcagc
     1141 attttcttct tcagaccttt ggaaaaaacg tgctaactca agatctgtat gctttttcaa
     1201 gtattgctta tattccatca agccagaatc agaaaaacgg acaatatcag atattgtatc
     1261 aaatttttga gggccattta gataattttc ataaccagct tcaataatgg gtttcatcac
     1321 gaacaaagcc ataatcaatg caataccatt gagagtcata tttgatggaa cctgctgcaa
     1381 accaagtgca tttcgtacca ttacaaaaac aatagaaaac tttatataac aggtaccagc
     1441 agcaactaaa aatggtaata gcgtaaaaaa agaaagtgta gcgatgaggg acatgtcact
     1501 cagcatgaga ttactccttt accatccaag aactaatctc gataccataa ccatcctcaa
     1561 tagaaacaag ttccccatgc ccaaccaaag aaccatttac tttaatattt acatgtttaa
     1621 ctatgtcatc gggaaacttg aataattcgt tttctacata catttttagt tcattgattg
     1681 tcatattttt ttctaaaaga ataaagtcca cttttacatt gatatcatca taattgaata
     1741 atgccaaaga aacttctgtg tgttctgact caccattact ttctgacaga ttaatttttg
     1801 cctcattatt atcattcaca atataatccc caataataac ttgattacac aataataaat
     1861 tataaagttt ctgaactatg attatatcac cagtgcgaat tctttttaaa gtagccagcc
     1921 ttacattaga tgaacctaag caaaactcta aaagatgatg caatgagtca agagaaactt
     1981 gattacgtaa taccggcatc ttgtcaaaat aaataatacc taaagaagag gaaaaacagc
     2041 ataaatgact cccagtagtt gcatactgga tttcattcgt gatccggaaa gtaa
//
LOCUS       DAZOZX010000315         2092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZOZX010000315 DAZOZX010000000
VERSION     DAZOZX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.321"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHUPT_004328"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHUPT_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0821609.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHUPT_004329"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHUPT_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0821610.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZOZX010000316         2090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZOZX010000316 DAZOZX010000000
VERSION     DAZOZX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.322"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..797
                     /locus_tag="ACHUPT_004330"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACHUPT_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACHUPT_004331"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACHUPT_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACHUPT_004332"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACHUPT_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          505 c          632 g          437 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagcagctt
//
LOCUS       DAZOZX010000317         2087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZOZX010000317 DAZOZX010000000
VERSION     DAZOZX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.323"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..100)
                     /locus_tag="ACHUPT_004333"
     CDS             complement(<1..100)
                     /locus_tag="ACHUPT_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821611.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYL"
     gene            complement(227..1876)
                     /locus_tag="ACHUPT_004334"
     CDS             complement(227..1876)
                     /locus_tag="ACHUPT_004334"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0821612.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          504 a          549 c          531 g          503 t
ORIGIN      
        1 cgaggtaatc tcccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat
       61 caagagcctg tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt
      121 acacagccac tggatcggtc aactgatcct taactgatcg gcattactcc tcctgaagag
      181 gataatttta cagccagcag gaagttagcg gctggctgta aaaatattat ggttcgccgt
      241 acaaaccaat taaacggcgt accacaccgt tagtccagcc aaacccatcc tgcaacggat
      301 actcgccacc gccgccttcg cggggaacac catcggcaat atggtatttc tcgatcattt
      361 tgtgctgttc cagatagaac tgattcaccg ttttcagcca gctgcgcgcg atttcatcac
      421 ccagaaggtc atcgccatac attttaaatc cctgaattgc catccattgt aacggtgccc
      481 agccgttggg tttatcccac tgttcaccgg tttcgtactc gcttgccaaa atcccgccag
      541 gtgtcagtaa ccggctgcgt acggcgtttg ccagacgatc ggcctgttca tggttcgcca
      601 tgccgacata gagcggcaca atggcggcag cggaaaacag cgccagttgt tcgcgtcgcc
      661 agtcgtaatc gcggtagatg ccgttttcat catcccagag gtaacggttt accgcatcgc
      721 gacgggcact ggccttctgg cggaacagcg cttctgtctc tttctcgcct ttcagcgccg
      781 agatgttggc gatggcgctc tccagtttaa acaggaaggc attcagatcg atggggatga
      841 actgggtggt acgaatgctc gccagacgac cagtatcacg cagccaacgg gaagagtaat
      901 cccaaccgga ggccgctccc gcgcgtaaat cgcggtacac ctcgttgggc gggcgaccag
      961 aatgtttcgc ggtttcaacg tcctcaagcc aggattcgtc acgcggcgtg tcgcgatcgt
     1021 cccagtaacg gttgagcagc gatccgtccg gcatccgcac aacatggcga taggcctgat
     1081 tagggatcaa cgattctgca ccgtccatcc agaaggcata ttccatttta aggtggtcga
     1141 gatagcggcg cgcaccgcgt acaccatctt cttcaaacaa ctccaccatc agcgcaaaaa
     1201 ccggtggttg cgagcggctc aaataatagg tgcggttgcc gttggggatg tgaccgtagt
     1261 tttcgatcat ccaggcgaag ttatcggcca tgcatttcag caaatcttcc cgaccacttt
     1321 ccgccagtcc cagcatggtg aaataggaat cccaatagta ggtttcgcta aaacggccgc
     1381 ccgggacaat atatgactgc ggtagcgcca gcagagaaga ccacggaatg tgatcctgtg
     1441 gttcgcgggt tagcaccggc cacagctggt cgatatgctc tttcagggaa ttttgcgggt
     1501 ccgatacata ctcgctggag tagacctccg gcagccagaa gtgattttca acaaacttgc
     1561 gcaagtcaaa atcacgatga cggcgcactt tacggtagcg gattaaaata tccagcgggt
     1621 ccattttggg tgcgcagtcg ggaaaggttt tactgtcggg gaaaattttc gccgactgaa
     1681 catgctcgaa cagttcgaga tagcgatcgg caggcgtcag cgcatcagag gcgggcagcc
     1741 cttcaatcat ctcgggttcc ggttctgcct cgatcatctc atccagtttt aattcatacg
     1801 ggtccagttc atagcagaga tcgacctcga tcatcagttc gtctggatta gggttttgaa
     1861 ttttctgatt gagcataagg atccaacctc cgaaattcgt cgctaattat cgggcttttc
     1921 tttaagggta gtcattcgct ccgcctggtg ggcgacaaag cgtgcggtgg atcacgttta
     1981 ttaacttgtc aaaaaatata acgcactaat atcaatggaa tttataaaaa tacggatttg
     2041 gtgttgtttt tcattttcag aacattcaat cctgctctcg ccgcaat
//
LOCUS       DAZOZX010000318         2077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZOZX010000318 DAZOZX010000000
VERSION     DAZOZX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.324"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUPT_004335"
     CDS             complement(60..1025)
                     /locus_tag="ACHUPT_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0821613.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1867..>2077)
                     /locus_tag="ACHUPT_004336"
     CDS             complement(1867..>2077)
                     /locus_tag="ACHUPT_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821614.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          574 a          548 c          432 g          523 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga acccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc aggatagaca
     1801 gagcactaga gattgcatgc aatctcgtac ctatcacctc agcctgccgc tggctctttt
     1861 ccagaactaa gaatgcgttc ttaaaaatcc aaacttgata ccgtttaaat ctattttcac
     1921 aaaaagagtt tttaccctct cgtttgtttt ctgaatatat aaaattaatt catcctcaaa
     1981 accttcgtgc ggatgaaaac caaatgtgcc aaaacttaat ccatcaataa gcaaaataag
     2041 gtttttaaac ctgtcaggaa tcaccgcttc acttctt
//
LOCUS       DAZOZX010000319         2073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZOZX010000319 DAZOZX010000000
VERSION     DAZOZX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.325"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..951
                     /gene="virK"
                     /locus_tag="ACHUPT_004337"
     CDS             1..951
                     /gene="virK"
                     /locus_tag="ACHUPT_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0821615.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1016..1960
                     /gene="lpxM"
                     /locus_tag="ACHUPT_004338"
                     /gene_synonym="msbB"
     CDS             1016..1960
                     /gene="lpxM"
                     /locus_tag="ACHUPT_004338"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0821616.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          609 a          397 c          462 g          605 t
ORIGIN      
        1 atgttttcta taagtaactt atcatttatc ggtttcctta aaaggattat tttttcctca
       61 gattcactac cggggaagtg ggaacacaga aaatttcggt tcatgtacat tttgcgatgt
      121 tctataaatc cggttgtcag tattcgatat tattatgaac tgcgttcctt gccgtgcatt
      181 gaggatattc tggcaataca ccccacgttg ccagcaagaa ttcatcgccc ttatttgcat
      241 aaaggggggc gcgcctggac ccgtggacaa tatattcttg agcattatcg ctttgtgcaa
      301 aatttgccag aaaaatattc taaatttttg tttccacaaa aatcggtatc attagtccaa
      361 ttcatcggga aagatggaga gaattttgat atccagtgtt ctcccagtgg ttttgaccgg
      421 gaaggagagt tgatgttgtc attgttttac aataaaacag ttattgcccg gttaacattt
      481 tcagtcattc tgactcaaaa tggacacatt gcttttattg gcggattaca gggagcccct
      541 aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg gattttccca
      601 aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgcaatat ctctgagtgt
      661 ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta tcagaaacgc
      721 aaaacctttg ttgcggtcta tagtgatttc tgggagtctg tagcaggaaa aacctgtggt
      781 gactggtaca gattaccaac ccaggtgatt cgaaagccgc taagcgatat agccagtaaa
      841 aaacgctctg ggtaccgaaa acgatacgct ttgctggatt atatccatga aactactatc
      901 cgctctttgg atgcatatcc tgtacactca gaacatcaga atttaaatta aggtttgatg
      961 tattctctga ataaaacagt aatgataatt ttggtagagg cattcgcact aaataatgaa
     1021 aaaatataaa tctgagttta ttcctgaatt taagaaaaat tacctttccc ctgtttactg
     1081 gtctacatgg ttccttttgg gaatgattgc aggtatttca atgtttcccc cttcattcag
     1141 agatcctgtc ttggcaaaaa tagggcgttg ggcgggcaga ttgagcaaaa aagctcgtcg
     1201 cagggcgacg attaatttat cgctttgttt cccagaaaag agtgatacag aacgggaaat
     1261 aattgtcgac aaaatgtttg tcacagcatt gcaatccata gtgatgatgg cagaactggc
     1321 aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg aaattcttga
     1381 ggaaattcga cacaataata gaaatgtgat ttttctggtt ccccatggct ggagcgtgga
     1441 tattcctgca atgttgctgg cagctcaggg aaaaaaaatg gccgccatgt ttcatcagca
     1501 acgaaatcca gtgattgatt atgtctggaa ttcagtacgg cgtaaattcg gggggcgctt
     1561 acatgcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg gatactgggg
     1621 atattacctc ccagatcagg atcatggtcc tgaatacagt gaatttgctg atttttttgc
     1681 gacctataaa gcgacattac caatcattgg acgtctgatg aacatcagtc aggctatgat
     1741 tataccactt ttcccggttt atgatgaaaa aaaacatcta ctgactattg aaattcggcc
     1801 accaatggat gcttgcattg ccagcgcgga caataaaacg attgcccgac aaatgaacaa
     1861 aacagtggaa attttggtgg ggccacatcc ggaacagtat gtctgggttt taaaattgtt
     1921 aaaaacacgc aaatcaaacg aagcggaccc gtacccttga aattataaag tacaagtatt
     1981 tccactagtt gtttgtaagc gtacagcctg aaccgtctgg tcagaatctg acgaattaga
     2041 caaagtggtg tccaccaaat aagtagtggg aac
//
LOCUS       DAZOZX010000320         2065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZOZX010000320 DAZOZX010000000
VERSION     DAZOZX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.326"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1154
                     /locus_tag="ACHUPT_004339"
     CDS             <1..1154
                     /locus_tag="ACHUPT_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821617.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACHUPT_004340"
     CDS             1833..2051
                     /locus_tag="ACHUPT_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR0821618.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
BASE COUNT          644 a          374 c          472 g          575 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcat
//
LOCUS       DAZOZX010000321         2040 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZOZX010000321 DAZOZX010000000
VERSION     DAZOZX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.327"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(13..901)
                     /locus_tag="ACHUPT_004341"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACHUPT_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          475 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
//
LOCUS       DAZOZX010000322         2029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZOZX010000322 DAZOZX010000000
VERSION     DAZOZX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.328"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1824)
                     /locus_tag="ACHUPT_004342"
     CDS             complement(<1..1824)
                     /locus_tag="ACHUPT_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR0821619.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHUPT_004343"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHUPT_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821620.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZOZX010000323         2028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZOZX010000323 DAZOZX010000000
VERSION     DAZOZX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.329"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(71..805)
                     /locus_tag="ACHUPT_004344"
     CDS             complement(71..805)
                     /locus_tag="ACHUPT_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0821621.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            complement(805..>2028)
                     /locus_tag="ACHUPT_004345"
     CDS             complement(805..>2028)
                     /locus_tag="ACHUPT_004345"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0821622.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
BASE COUNT          567 a          522 c          441 g          498 t
ORIGIN      
        1 tattactaac ctcaccacgc atgctcagcg atttcgtcat cgaaaaagag agttatctat
       61 gacgcatcaa ttatactttt tgctttgttc gcatattgtt acatctttca taccttgacg
      121 catctcttcc caacgcgggt atcgcctgtt gtataagttg agcattacgt actaaccaga
      181 cgtactcact tttattcctg gtgcctaatc gttctcgaat tgcggactca tgggcgctaa
      241 cggtcttgag gccaatacca cagatttctg caattttacg tgtgctaatc cccttcgcgc
      301 ggagtaaaag aaccctccat tgcactcttc ccaggcgttt tataaacaaa ggaccaggca
      361 aacaatgagc atttaacacc ttccgtaatg aagataataa aatatccact tttgcactcc
      421 catgagtaag ccatactcgg gaaagaggcc cgatatcatt cgtcattcct tcaggaatag
      481 catcgctaat aacgagttgc cgcatcagac ggtatcgacg acaaaccttc acaatatgtc
      541 cggctaattc aggcaaacca tcgtgacgca tttttaccca tatcacgatg ctactgtcag
      601 tcagaacatt catatcagtg ggtaaggaag tgaaaacctg aagtttcact cccccaacac
      661 gcttgagcaa actgcacacg ccgcaaatgg caaaaagatc caggccaatc accgtgacat
      721 ttgtccatgt ggtattttct ggcgagagaa tattacagtt gatgtttgtg ctttctaatt
      781 tcttatacgt cagatcgtta accattagtt tcccccgcga gtaaaacgtt cgtcatttac
      841 aaatacccga gctgcgcgtt ttcttatttt ttcagctgtc agataatcac cgcgcctgtg
      901 cgcaatgaat atcattgcag cgcttacgtc tgcatcattg tggattgcca accatttatt
      961 tccccacaca gtgaaaagag acatgctttc cgtatcgcga acgaaccccg gtttattcgc
     1021 aataagcaga tgctgatcgc gctcaagacg ttctgcctgc gtcaggtatt tccagctatg
     1081 ttccggaagg cgagggataa atccttcgtt catatcagct tcagctctgg tcagttcccg
     1141 ctgtgcatca aatcccgcta ccaggatgac cagcgcggcg gtcataatga gcacaatgac
     1201 aggcagaata cccaaccgga ctgttttagg tatacgtacc tgattcatca cgttttccgg
     1261 cattccgatg cggaaaagga gcagcaacag catcaaatgg ggaacagacg tatagagagg
     1321 atattcggtc atcatatgaa tgactaaagg caaccccatt aaggcaataa accacttttc
     1381 acattgatgg tgcgcgttat tgtttgtcat tttcaggcgg catatacacg gccacagcca
     1441 aacgcctgat agcagcaaaa aaccaaacaa ggcgacgatg ccaccttcag ccgttacgaa
     1501 cagcagttcg ttatggggat gcgtagccgt atcactctct atgtttgtca cgcccacatc
     1561 ctctaaggct tgcgggaagg tcgcttcaaa gctgcctaac ccccaaccgg ttaaggggcg
     1621 ctgttgaata agacttatgg cacctttcag aatacgtata cgctggtcat tcgatgatga
     1681 atgcgtaaag tcagccgttg tctcccctgg catggcatag tttgagatac cgacacacca
     1741 gcacagcaag gaggcgacga agatcaagcg caacagaccg ctaaggggac gtccgcgcca
     1801 gcggggtctg tccgcgagag cggccagcac gatgacgcaa aataaaccaa gaaggccgac
     1861 acggctttgt gtaaggacta aagctgtaga cagagggtat aacgacaaca aacatatcaa
     1921 cgtcagagaa cgagtacgcg ccgttaaagc cagatacaac gctacgccgc aacctgttgc
     1981 cagccagctc gcaagaacat ttcgctgctg gaaaatgcca tacggaaa
//
LOCUS       DAZOZX010000324         2025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZOZX010000324 DAZOZX010000000
VERSION     DAZOZX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.330"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..334
                     /locus_tag="ACHUPT_004346"
     CDS             <1..334
                     /locus_tag="ACHUPT_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR0821623.1"
                     /translation="YRFNFYDQHGFPGFWGATPEYKAAFKALKVRQRLTIQMNFIAFF
                     CSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVAAYVAVNTNKWFYEKEV
                     KGLNTWSL"
     gene            complement(375..1325)
                     /gene="corA"
                     /locus_tag="ACHUPT_004347"
     CDS             complement(375..1325)
                     /gene="corA"
                     /locus_tag="ACHUPT_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0821624.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1477..1533)
                     /gene="ysgD"
                     /locus_tag="ACHUPT_004348"
     CDS             complement(1477..1533)
                     /gene="ysgD"
                     /locus_tag="ACHUPT_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0821625.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1695..>2025
                     /locus_tag="ACHUPT_004349"
     CDS             1695..>2025
                     /locus_tag="ACHUPT_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821626.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          537 a          491 c          491 g          506 t
ORIGIN      
        1 ataccgattt aatttttatg atcaacatgg ctttcctgga ttctggggag caacaccaga
       61 atataaagca gcatttaaag cattaaaagt aagacaacga ttaactattc aaatgaattt
      121 cattgctttc ttctgttcct ggatctattt atttgttctg ggcttgtgga aaaaagcaat
      181 tatagttcta ttacttggca ttctatctct atttgttggt gcattaattg gcgttaacat
      241 tctgggaatt gccgtagcag cctatgttgc tgttaacacg aacaaatggt tttatgagaa
      301 agaggtaaaa gggttaaata cctggagcct ttgaataaca ggtggcttag ccaggctaag
      361 ccaccgcctt ctttttacaa ccagttcttc cgcttaaagt acagatacgg tgccaggccc
      421 gcgaggatca taaagataat cgcgccaggg tagccgaaac tccacttcag ttctggcata
      481 aactcaaagt tcatcccgta gctggaagca accagcgttg gcggcaggaa cacgacggat
      541 accaccgaga aaattttgat gatgcggttc tgctcgatgt tgataaaacc cattgccgcc
      601 tgcatcagga agttcacctt ctggaacagg gattcgttat gcggcagcag ggattcgata
      661 tcgcgcagga tttcacgcgc ctgctccagt tgcccacccg gtaaacgcgc tttacgcacc
      721 aggaagttga gagcgcgctg ggtatccatc agacacaggc gaactttcca gccgatatct
      781 tccagttccg ccagcgtgga gagcgcctcg tcgtactcat cgccctgatg cccttccata
      841 atcacccggc tcaactgctc caggtcgcta tagatatttt caatttcatc tgccaactgt
      901 tcgattttgg tttcgaacag atccagcagc aactcgtagg cgttaccgtc taccatcgac
      961 tggctacggg cacgcatacg atacagacga aaagcgggca gttcacgctc acgcagagta
     1021 aacagacgac catcacggat ggtaaatgcc acagtggagt taccggcgtg atcttccgca
     1081 tcttcaaaga agaagaagga gtgaatatgc aggccgtcgt cgtcttcaaa gaaacgtgcc
     1141 gatgcttcga tgtcttccag ttccgggcgg gttgccaggc tctggccaag ttcagattgt
     1201 acgcgcagtc gctcgtcgtc gtccggttcg acaagatcaa tccatactgc atttacaagg
     1261 ggttgtgact cttcgacttc cagccgggtc agtcggttat tttccagttg aaatgcgctc
     1321 agcatgaccg ggactcccaa tgcttaaaat atcggacagt tcggtgggca cacagaaaca
     1381 aattgggttt cagaccatta aacagcctga ctcagcgcga cgggaaaaat tgaggtcgct
     1441 gacaaccgct aaggctatca gcaaaaaggg atagccttag gagttgatcc tggatgacag
     1501 gatagtgagc cagtatctac tgggtgtgtc caaggcgaat gtcctcttag agtgatcgtg
     1561 cgcgcatgtt acgccagcaa aattttgccg tcaacacgca acagaacacc acagaacaat
     1621 agttgaactt cgcgcttttt ggtactcgtc aaggtcgcta acaggataaa aaaatcccac
     1681 atctaggacc tataatggcg catcggctac tcattggtaa aggaatgatc accttgaatc
     1741 tcaaacggat ttttctcacc ctcaccttac ttcccttgtt tgctgttgcc gctgatgatt
     1801 gcgcactctc agatccgacg ctgaccgtac aggcgtatac cgttaatcct caaacagagc
     1861 gggtgaaaat gtactggcaa aaagccaatg gcgaagcgtg gggaacgtta catgctctgc
     1921 tggcggatat taatagtcag ggtcaggtgc agatggcgat gaacggcggc atctatgatg
     1981 aaagctatgc gccgctcggt ttgtacatcg aaaacggtca gcaga
//
LOCUS       DAZOZX010000325         2022 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZOZX010000325 DAZOZX010000000
VERSION     DAZOZX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.331"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            31..1554
                     /locus_tag="ACHUPT_004350"
     CDS             31..1554
                     /locus_tag="ACHUPT_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0821627.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            1686..1905
                     /gene="ymgF"
                     /locus_tag="ACHUPT_004351"
                     /pseudo
     CDS             1686..1905
                     /gene="ymgF"
                     /locus_tag="ACHUPT_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
BASE COUNT          536 a          434 c          452 g          600 t
ORIGIN      
        1 tgtattaaac cctttggccg ggacaagcaa atgcgcaata cactcatacc catcctcgtt
       61 gcgatatgct tgtttattac cggtgtcgct attttgaaca tccagctctg gtattccgca
      121 aaagcagagt acctggcggg agcaagatat gccgccaaca atatcaatca tatacttgaa
      181 gaagcgtcac aggcgactca aacagcggtt aacattgccg ggaaggaatg cgacctcgag
      241 gagcaatatc agcttggcac tgaagcagct ctgaaacctc acctgcgcac aatcatcatt
      301 ctcaaacagg gaatagtctg gtgtacatcc ctgcctggga atcgggtcct gctgtctcgt
      361 attcctgttg tcccggacag taatttactg ttggctccgg caatcgacac cgttaataga
      421 ttacctatcc tgctctatca gagccaattt gcagatacgc gcattttggt tacgataagt
      481 gatcaacata ttcgcggggc gcttaatgta cccttgaaag gggtaaggta tgtattacgc
      541 gtggcggatg acattattgg accaacggct gatgtgatga cgcttaatgg acattatccc
      601 tataccgaga aggttcactc cacaaaatat catttcacta ttatctttaa cccgccgcca
      661 ctctttagct tctacagact tatcgataaa ggctttggga ttttaatatt tattctgtta
      721 atcgccagcg ccgctgcctt cctgcttgat agatatttca ataaaagcgc aacgcctgaa
      781 gagatcctgc gacgggcaat aaataatggg gagatcgtcc ctttttacca acctgtggta
      841 aatggtcggg aagggacatt gcggggagtt gaggtgttag cccgctggaa acaacctcac
      901 ggtggatata tttcacccgc ggcatttatt ccacttgctg aaaaatccgg attaatcgtt
      961 ccgcttacac aaagcctgat gaatcaggtt gccagacaga tgaacgctat cgcgagtaaa
     1021 ctgccggaag gttttcatat tggaattaat tttagcgctt cgcatattat ttcgccgacg
     1081 tttgtcgacg agtgtttaaa tttccgtgac agttttaccc gccgcgattt aaaccttgtt
     1141 ctggaagtca ccgagcgtga gccattgaat gttgatgaaa gtctggttca gcggttgaac
     1201 attctgcatg aaaatggttt tgtcatcgcg ctggatgatt tcggtactgg ctactcaggg
     1261 ctttcttatc ttcatgacct gcatattgat tatatcaaaa ttgatcacag tttcgttggc
     1321 cgcgtaaacg cagacccaga atcaacccga attctggatt gtgtattgga tctggcgcgt
     1381 aaactttcga tcagtatcgt cgctgaaggt gtcgaaacga aagaacaact tggctatctg
     1441 aaccaaaata atatcacatt tcagcagggt tattatttct ataaacctgt tacatacatc
     1501 gacctggtca aaattatcct ttctaaaccg aaggtgaagg ttgtggttga gtgaaaattg
     1561 atcagtaagg ccatagtgcg gtgtaattat agacagctaa ttagctcgtt gcctcttgtt
     1621 actattgttc attattttgt ttgctataat tgtttgaaag ttttgacagg attgccatta
     1681 gtagcatgaa taatagtaat aatctggatt atttcactct ctatatcatc ttttccattg
     1741 catttatgct gatcaccctc ctggtcatcc ttattgcaaa acccagtacc gggctgggag
     1801 aagtgcttgt gacgataaat ttgcttaatg cccttgtttg gctggcgatc aatctggtta
     1861 atcgatttaa gagaaagact cgttagccac agggatcagc aataatcttt cagtttctca
     1921 ctgtcagtat gcggctgaat gggttgctgg cagtgaacgc ctggatcatt gaaggaaagg
     1981 aattattgcg caaatagttg tcaaccttgg tgttatcaca gt
//
LOCUS       DAZOZX010000326         2014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZOZX010000326 DAZOZX010000000
VERSION     DAZOZX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.332"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(295..369)
                     /locus_tag="ACHUPT_004352"
     tRNA            complement(295..369)
                     /locus_tag="ACHUPT_004352"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Gly,seq:tcc)
     gene            complement(373..448)
                     /locus_tag="ACHUPT_004353"
     tRNA            complement(373..448)
                     /locus_tag="ACHUPT_004353"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(413..415),aa:Thr,seq:tgt)
     gene            complement(451..527)
                     /locus_tag="ACHUPT_004354"
     tRNA            complement(451..527)
                     /locus_tag="ACHUPT_004354"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Arg,seq:tct)
     gene            complement(529..604)
                     /locus_tag="ACHUPT_004355"
     tRNA            complement(529..604)
                     /locus_tag="ACHUPT_004355"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(569..571),aa:Ile,seq:cat)
     gene            complement(781..1470)
                     /locus_tag="ACHUPT_004356"
     CDS             complement(781..1470)
                     /locus_tag="ACHUPT_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR0821628.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1467..1826)
                     /locus_tag="ACHUPT_004357"
     CDS             complement(1467..1826)
                     /locus_tag="ACHUPT_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0821629.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1826..2014)
                     /locus_tag="ACHUPT_004358"
     CDS             complement(1826..2014)
                     /locus_tag="ACHUPT_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821630.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          509 a          551 c          437 g          517 t
ORIGIN      
        1 cgagcgctgt acaaaaaacc gccagccaag gcagttaaga gtgtgttccc ggggtttgct
       61 tagaatattt tgtaagttgt ccgaagagat atttacaaca ccagaatgat gaatcatcag
      121 tcccctgcca gaggttcact acgtaacctg accgacaatg caccattctg gtgctgaaaa
      181 aagagcactg gaactgcaac aaaacaacat cacagaacag gagatggtaa ggagtcagac
      241 attcccacac aatattgtgt cagtgcaaat aacaaccttc gtctcaaatc tcgctggagc
      301 gggcagcggg aatcgaaccc gcatcatcag cttggaaggc tgaggtaata gccattatac
      361 gatgcccgca tatggtgccg actaccggaa tcgaactggt gacctactga ttacaagtca
      421 gttgctctgc ctgctgagct aagtcggcgc tggcccacca ccgaggactc gaacctcgga
      481 ccgtcaactt agaaggttga tgctctatcc ggatgagcta gtggtggttg gtggcccttg
      541 ctggacttga accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag
      601 ggccggaggc agaataataa ccatatgtca tcacatctgc aaactcatct gaccaccagc
      661 acgtttaacg tcctgtgcca tttttcaggc ataaaaaaac cgctcggcgg cgggttttgc
      721 ttactttacc atcgcgtaca aaatcggcaa agtatcagat ttgcatgaga tatatgcctt
      781 ttaatctact tttgcaatac tttgctttga aaatgccgct ttttgttctg aacgtgttct
      841 catcacaaac aataaagcct cactatccag tcggtgaaaa atgtgtttca ttacaaccca
      901 gtgaccagta aatgttttgg accagttttt ggttgttact cccgccagta atgccagctc
      961 ctgatattca taaccttccc caccaaaaag ttctgctttt accgcctgcg ccgccagcca
     1021 gattaatttt ttcaggcgtt cctgtgtttt ctctgcaatt ttcctagtgc cagaatgagc
     1081 cttaaactct tcccacgccc agtgggttat ggtgatctga tactcccagc aaatacttcc
     1141 gctgtaacac cacaacaacc aggctttata gtgctcttca agagacagaa cagcccgccg
     1201 ccacgatgat gtcgaaaacg caacctgact gaccagagga attgacgccc ccttcgccag
     1261 tgattgtttt cccgggattg gtggattatc ccgcgttatc atttttccgg tcacctcatc
     1321 gcggtaccgg atttttttac gcctgtaacg ccctgtatcg aacatggcat tctcctgcca
     1381 ggcttcaagc tgaccttttg tcgccccact caaatcagcg gtggcgataa tgagctgctc
     1441 acgaacaaac tgtaaatact ggttattcat gcacactcca gctctgtgat ttttatcccc
     1501 aaccgaccac caggaacagg cagtccgcgc acaatattgg tttcatcaaa ctgctcgtcg
     1561 tcgataagca accccgcatg tgtcagtgca tccagtggtg ctttcaggat attgtccagg
     1621 tcacgacgcc gcttatccgg tggctctgca ataatcttta tcgccagtct tccggacagg
     1681 cttaatttca gtcgctgctg acggacaata agcgccacat cccggcgata acgctcaccg
     1741 gcttttgata caaaatatgt gctgccacga cgtcgccagt aagtattcac cgtcggcgga
     1801 taaggtaaaa taaactcatg gcgcatcagc gcagcacctc ctgcaccagc ttttcaaact
     1861 ttccgactct ggtttccagc tctgccacac agtccaccag ctcatctact gctttctgtg
     1921 cgcgatgctt cgcctgcatc agttcccgaa gcgcgggtac catattgagg tagcctgagt
     1981 ttaacggaca ctccttcctg aaatagaatg gcat
//
LOCUS       DAZOZX010000327         1969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZOZX010000327 DAZOZX010000000
VERSION     DAZOZX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.333"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHUPT_004359"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHUPT_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHUPT_004360"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHUPT_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHUPT_004361"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHUPT_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR0821631.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACHUPT_004362"
     CDS             1708..>1969
                     /locus_tag="ACHUPT_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0821632.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTACGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          433 c          462 g          514 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgcctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAZOZX010000328         1926 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZOZX010000328 DAZOZX010000000
VERSION     DAZOZX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.334"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUPT_004363"
     CDS             <1..203
                     /locus_tag="ACHUPT_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821633.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACHUPT_004364"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACHUPT_004364"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0821634.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          476 a          477 c          448 g          525 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaat
//
LOCUS       DAZOZX010000329         1913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZOZX010000329 DAZOZX010000000
VERSION     DAZOZX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.335"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(854..1252)
                     /locus_tag="ACHUPT_004365"
                     /pseudo
     CDS             complement(854..1252)
                     /locus_tag="ACHUPT_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1255..1621)
                     /locus_tag="ACHUPT_004366"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="ACHUPT_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1913)
                     /locus_tag="ACHUPT_004367"
                     /pseudo
     CDS             complement(1623..1913)
                     /locus_tag="ACHUPT_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          536 c          459 g          493 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgcggacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tga
//
LOCUS       DAZOZX010000330         1903 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZOZX010000330 DAZOZX010000000
VERSION     DAZOZX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.336"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            57..293
                     /locus_tag="ACHUPT_004368"
     CDS             57..293
                     /locus_tag="ACHUPT_004368"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821635.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            268..1899
                     /locus_tag="ACHUPT_004369"
                     /pseudo
     CDS             268..1899
                     /locus_tag="ACHUPT_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          513 a          416 c          524 g          450 t
ORIGIN      
        1 cgaattattt gttttggtaa ccctttatca tacgcaatat ggttggtgtg cgaaagatga
       61 gtaacgattg gcatcgggcc gacgtgattg cggccctcaa aaaaacagga acccctctgg
      121 caaaattgtc cagacgttca ggttattcaa ccgggacgct tgcaaatgca ttagtcaggc
      181 catggcctaa aggtgaacgc attattgctg acgccttaaa catgaaacct gaggaaattt
      241 ggcctagtag atatctaagg gatcacaatg agagagtgga tatcagcaat tgaatgcatg
      301 ggtttatctg gtttacctgg ttcattaatg ggggttcaca aaagagctca gagagaagga
      361 tggctcaaga gaagaagaga aggaattaga ggggttgctt acgaatacca tatatcttct
      421 cttcctaaca ttgctcgagc tgaagttttg ctccaacaag gaaaatttaa aaccagtcag
      481 ggatgttttg aaatcgcccg ccccacgctg gaagctcatg attatgatcg tgaggcacta
      541 tggagcaaat gggataaagc cagcgattcc cagcgcagcc tggctgaaaa atggttgccc
      601 tcaattcagg ccacagacga aatgctgaac caggggattt caacgaaaac ggcttttgcg
      661 accgttgcag ggcattacca ggtcagcgca tccactttgc gggacaagta ttaccaggta
      721 cagaagtttg cgaagcctga ctgggcggct gcacttgttg atggacgtgg agcatcccgt
      781 cgcaatgttc acaaaagtga atttgacgag gatgcctggc agtttctgat tgcagattat
      841 ctgcgaccgg aaaaacccgc cttccgcaaa tgttatgagc gtctggaact ggcagcccgc
      901 gagcatggct ggagtattcc ctcccgtgcc acggcctttc gccggattca gcaactggac
      961 gaggcaatgg ttgttgcctg tcgtgaaggt gaacatgcac tgatgcatct gataccggca
     1021 cagcagcgaa ctgtggaaca cctggacgcc atgcagtgga tcaacggcga cggttatctg
     1081 cataacgtct ttgtacgctg gtttaacggt gatgtgatcc gtccgaaaac atggttctgg
     1141 caggatgtga aaacccgaaa aattctgggc tggcgctgcg atgtgagcga gaacattgat
     1201 tcaattcgcc tctcgttcat ggatgttgtg actcgctaca gtatcccgga ggattttcac
     1261 atcaccattg ataacacccg tggtgctgcg aataaatggc tgacgggagg cgcgcccaat
     1321 cgctaccgct ttaaggtaaa agaggacgat ccaaaaggac tgtttttact gatgggtgcg
     1381 aaaatgcact ggacaagcgt tgttgccggt aaaggctggg gccaggcaaa acctgttgaa
     1441 cgtgctttcg gtgttggtgg gcttgaggaa tacgttgata agcatccggc actggctggc
     1501 gcatatacgg ggccaaatcc gcaggcaaaa cctgataact atggcgacca cgctgttgat
     1561 gcagagctgt ttctgaaaac ctttgccgaa ggtgtggcga tgttcaatgc cagaacaggc
     1621 cgtgaaacag aaatgtgcgg gggcaaactc tcgtttgatg atgttttcga gcgtgaatac
     1681 gccagaacga ttgtgcgtaa gccaaccgaa gaacaaaaac ggatgctgtt actgcctgcc
     1741 gaggcggtga acgtttcacg caaaggcgag tttacgctta aagttggcgg ctcccttaaa
     1801 ggcgcgaaaa acgtttatta caacatggca ttaatgaatg ccggagtgaa aaaagttgtg
     1861 gtcagatttg atccacagca gttacacagc acggtttatt gag
//
LOCUS       DAZOZX010000331         1872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZOZX010000331 DAZOZX010000000
VERSION     DAZOZX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.337"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            198..377
                     /locus_tag="ACHUPT_004370"
                     /pseudo
     CDS             198..377
                     /locus_tag="ACHUPT_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(394..1084)
                     /locus_tag="ACHUPT_004371"
                     /pseudo
     CDS             complement(394..1084)
                     /locus_tag="ACHUPT_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1163..1255
                     /locus_tag="ACHUPT_004372"
                     /pseudo
     CDS             1163..1255
                     /locus_tag="ACHUPT_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1278..>1872)
                     /locus_tag="ACHUPT_004373"
     CDS             complement(1278..>1872)
                     /locus_tag="ACHUPT_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR0821636.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          463 a          435 c          459 g          515 t
ORIGIN      
        1 tatttatgga ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac
       61 tgaattcaac attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat
      121 catattttag tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa
      181 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg
      241 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
      301 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
      361 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
      421 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
      481 ccgcttgatg cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg
      541 ccggtcaggt gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc
      601 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
      661 cgtggcccgc aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc
      721 cagtgttgtt gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg
      781 gcaacatcag cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg
      841 tccgtatgac ggtattgatg ccgattttca gtgtcctggc ggtatcacgg ccccctgcgc
      901 cgttatgtgc catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt
      961 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
     1021 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
     1081 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
     1141 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
     1201 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taatctaaat
     1261 agctgcgctt aataccgcta cacttttgcc agcccatgtt tgcctccggg gaatgggctg
     1321 acggtttcca taaaatggcg aacttttttc aacagttgcc acattgagcg gcactgatga
     1381 ttacgcgtta ttgtgtcgtg aagtgcctgc catagccgtt caacatgatt cacccatggc
     1441 gagtaaaccg gctgataaat gcccctgaac ttcgggttct ccttcagcca gctctgtgtt
     1501 tcccggcttt tgtggataat gtagttgtcc acgatcagcg tgatggtttt cgcacgacgg
     1561 tatgtcgctt taagccgctt cagcaggctg atgaacagcg ccgaactttt gctgttgccg
     1621 cccacatagc tgactttacc tgtcccgctg tgcagcgctc cggccagata atatttttca
     1681 ttctgtcccg gcgtgaccac ccgtttttgc tgtccgcgca gttgccagtc cgcaccgatt
     1741 ttgggatgaa gatggatatc cacttcatct tcataaaaga ccggatgctc tgcgctgcat
     1801 tcgtccagtg ctttatggat tgctgccatc ttttcatctt tatgcgggtc acggatacgc
     1861 agagttggcg ca
//
LOCUS       DAZOZX010000332         1865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZOZX010000332 DAZOZX010000000
VERSION     DAZOZX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.338"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..448
                     /locus_tag="ACHUPT_004374"
     CDS             <1..448
                     /locus_tag="ACHUPT_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0821637.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
     gene            798..1160
                     /locus_tag="ACHUPT_004375"
     CDS             798..1160
                     /locus_tag="ACHUPT_004375"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HFR0821638.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1157..>1865
                     /locus_tag="ACHUPT_004376"
     CDS             1157..>1865
                     /locus_tag="ACHUPT_004376"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0821639.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFV"
BASE COUNT          541 a          377 c          430 g          517 t
ORIGIN      
        1 agagagtaaa tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt
       61 gaaaaaacaa atcggaaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa
      121 accagacgga acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg
      181 gaaagcggcg ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac
      241 ctgggcaagt tggctggttc aagccggagt cccgttatca gtgttacagg aaatgggagg
      301 ctgggagtct atcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga
      361 acacgcacgg caaatagact tgattctgaa cccatcggtc ccaaatttgt cccagtcaag
      421 aaataaggaa ggtactaatg atgtgtaact tattgattta aatggtgccg ataataggag
      481 tcgaacctac gaccttcgca ttacgaatct gtagcaccaa tcataactat ctgttttagc
      541 aagcattaac cgcattcact aagatatagt tgatggcaca aacagaaagt tgatgaatga
      601 tgttgccata tatatgtcac aaatacggca caacgatctt caaacatata gccacacatt
      661 cacagaagag cacaaagcct tgcaatccag tgcaaagatt tgtgtgccag agtttttctc
      721 atcaactacc gcaagtatcg atcgattgag acttggatga tagacttcat gcctttgatt
      781 attagctgat agaagaaatg ttaaagctat ttgcaaagta cacatctata ggcgtgctga
      841 acacacttat acactgggtg gtttttggtg tatgtatcta tgccgcgcat accaatcagg
      901 ctatggcaaa cttcgcaggt ttcgttgtgg ctgtgagttt tagcttcttt gcgaatgcaa
      961 aattcacatt caaagcatca actacaacga tgcgctacat gttatatgtc gggttcatgg
     1021 gaacactgag tgctactgtt ggatgggttg ctgatagatg ttcacttcct ccaattgtca
     1081 ctcttgttac cttctccgcc atcagcttgg tgtgcggttt cgtctattca aagttcattg
     1141 tctttaggga tgcgaaatga agatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1201 gataccaatt ttttataaaa cggtacgtga attcgaagag ttgaagccat atgaagtaga
     1261 aattgttttc ataaatgacg gaagcaaaga cgctacggag tcaatcatta acgctctggc
     1321 tgtttctgat ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1381 attgtttgca gggttagacc atacaaccgg cgatgccgtg attccaattg atgttgacct
     1441 gcaagacccg attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgacat
     1501 ggtgcttgct aaacgttcag accgctcaac tgatggacgg ctgaaacgta agacagctga
     1561 gtggttctat aaattacaca acaaaataag caccccaaaa atagaggaaa atgtcggaga
     1621 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctattgcctg agcgcaatct
     1681 tttcatgaaa ggcatactga gctgggttgg tggtcagaca gatgtcgttg aatatgtacg
     1741 cacagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1801 ggaaggtatc acaagttttt caacctttcc tcttcgcgta tggacttata taggcttgtt
     1861 tgttg
//
LOCUS       DAZOZX010000333         1849 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZOZX010000333 DAZOZX010000000
VERSION     DAZOZX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.339"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACHUPT_004377"
     CDS             complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACHUPT_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR0821640.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="ACHUPT_004378"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="ACHUPT_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR0821641.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="ACHUPT_004379"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="ACHUPT_004379"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR0821642.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="ACHUPT_004380"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="ACHUPT_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0821643.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /gene="rem"
                     /locus_tag="ACHUPT_004381"
     CDS             1253..1504
                     /gene="rem"
                     /locus_tag="ACHUPT_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR0821644.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="ACHUPT_004382"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="ACHUPT_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          467 g          465 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcggtt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaa
//
LOCUS       DAZOZX010000334         1847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZOZX010000334 DAZOZX010000000
VERSION     DAZOZX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.340"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..355)
                     /locus_tag="ACHUPT_004383"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="ACHUPT_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(355..1275)
                     /locus_tag="ACHUPT_004384"
     CDS             complement(355..1275)
                     /locus_tag="ACHUPT_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0821645.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1413..>1847
                     /gene="pgrR"
                     /locus_tag="ACHUPT_004385"
     CDS             1413..>1847
                     /gene="pgrR"
                     /locus_tag="ACHUPT_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0821646.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          461 a          454 c          426 g          506 t
ORIGIN      
        1 ggcacccccc acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta
       61 ctgcataatc gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac
      121 gaggctaccc accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt
      181 tttcagccaa tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag
      241 gatggacgct gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa
      301 tattggcagc aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag
      361 agtttcctca aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc
      421 ataaagatcc atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc
      481 ccgatcgtac aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg
      541 catcggctga gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt
      601 aaggctgcgc aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta
      661 atactcccag gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag
      721 tggcataatg gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc
      781 aatgtgtggc agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga
      841 accaatattg accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc
      901 attggctgta tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac
      961 ataagaaagc gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg
     1021 gttttctaac tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat
     1081 agtgacaaat cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac
     1141 gcccccacct gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg
     1201 agggaaatag atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac
     1261 tttattattc atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata
     1321 tcactataga tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt
     1381 taaatgatta atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga
     1441 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca
     1501 tggtgcagtc agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc
     1561 ttctgacgcg aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg
     1621 ttcttggccc tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga
     1681 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
     1741 taccagccat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg
     1801 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtc
//
LOCUS       DAZOZX010000335         1835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZOZX010000335 DAZOZX010000000
VERSION     DAZOZX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.341"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..362)
                     /locus_tag="ACHUPT_004386"
     CDS             complement(<1..362)
                     /locus_tag="ACHUPT_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0821647.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUPT_004387"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUPT_004387"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR0821648.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          550 a          474 c          377 g          434 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aataccctca aaaaccacct gaataattaa
     1801 ccattattgt taaaacaaca cgttgaataa tcaca
//
LOCUS       DAZOZX010000336         1834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZOZX010000336 DAZOZX010000000
VERSION     DAZOZX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.342"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHUPT_004388"
     CDS             complement(<1..182)
                     /locus_tag="ACHUPT_004388"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821649.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(323..877)
                     /locus_tag="ACHUPT_004389"
     CDS             complement(323..877)
                     /locus_tag="ACHUPT_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0821650.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(874..1164)
                     /locus_tag="ACHUPT_004390"
     CDS             complement(874..1164)
                     /locus_tag="ACHUPT_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0821651.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1164..1763)
                     /locus_tag="ACHUPT_004391"
     CDS             complement(1164..1763)
                     /locus_tag="ACHUPT_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0821652.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          422 g          471 t
ORIGIN      
        1 tggttatggg tgatcgggga gagagtttca acagagattt gagtggtcat aacgataact
       61 ccgtacattt ggaaattatc gccaccgacg acgccaatcg aactggtggc gaactgtgca
      121 gggttggcgt aaccgggtac ggaaaccggc gagcctttcg gctcccccac acagcccgcc
      181 ataaatcgcg aatgtgactg tgcaaacgat atgaaaaaag acgcgggcgc gtctcatatc
      241 gctccgtaaa catccgggac gccaatcccg acgccagatt ttgctggcgc gtgaggaata
      301 tagccccgga taacagattg agtcaacaaa cggtttttag atccccggaa gagaatgcat
      361 cacgcatcgg tagatagagc atatattcag caagatttag ccatacatct acccgactgc
      421 agcacgtccg gaaacaccac tcagggtgtg cgtcattcag caattcagcc attttacgct
      481 tagtcatccc ccgcccttca taccgttgcc ggaggacact aatcaatcca ggatgctctg
      541 ccagcacctc acttatgact ctatcaatac ataacgcctc tgcatcagta caatgcgcca
      601 gccaggtctt ttgcttgcca ttgatcatct ctcgcaaaaa cgcttccagc tcaggtttct
      661 ctattcccgc ttttttcatc ctgcgcaggg cttcattgac ggctgttttc gtcaattttt
      721 tggatgccag caactggtta aacatatttc ccgtcttacc gccgccaata tacgaccagc
      781 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      841 gttccccgct tttgcctgta tttgttgggt aaatcataaa taaccttcct ttctccagat
      901 ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      961 gctggttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa tcgctgcctg
     1021 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
     1081 agaagtttcg ggattgtgat tgcagtagcc aggaattctg acggtgcaca tctgcccccg
     1141 cgccgcttta cgtaaatcca ccattacgca aactccagta actgcgcggc cacattttcg
     1201 acttcctccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagtaca
     1261 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
     1321 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
     1381 acccactcgc ggaaagcgtc aaacgacttt agcaatgccg tatcccgggt tctgcatgtc
     1441 gcaactgtat taaggtattg ctctgcggca tcactcaggg ctggagtgtg ttcccgacct
     1501 actgattcgc acaggtaatc aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc
     1561 gccccaccga ccggagtcca gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg
     1621 tggaatgcgt agttacgcac acgcttaaag tctgcgtgta tccactcacc tattttgatt
     1681 tgatgcaaaa aatcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1741 ttgaccagtt gtatatgcgc catcgtagtt ctccgctggc gcagtagaat gggtgttcag
     1801 cccgttatgt agtataccag aattaatgcc aata
//
LOCUS       DAZOZX010000337         1826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZOZX010000337 DAZOZX010000000
VERSION     DAZOZX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.343"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..310
                     /locus_tag="ACHUPT_004392"
     CDS             <1..310
                     /locus_tag="ACHUPT_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821653.1"
                     /translation="GRIFSVSCFSDIPFVREHRIKLIMAGFLVYFWFFSFLVGWYKPQ
                     LKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRFLIFNTEQSGFYEINDVYVRK
                     "
     gene            544..672
                     /locus_tag="ACHUPT_004393"
                     /pseudo
     CDS             544..672
                     /locus_tag="ACHUPT_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            862..1266
                     /locus_tag="ACHUPT_004394"
     CDS             862..1266
                     /locus_tag="ACHUPT_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821654.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1329..1826
                     /locus_tag="ACHUPT_004395"
                     /pseudo
     CDS             1329..1826
                     /locus_tag="ACHUPT_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          511 a          406 c          454 g          455 t
ORIGIN      
        1 tggacggatc ttttctgtat catgtttttc agatatccct tttgttagag aacaccgcat
       61 taagttaatt atggctggtt ttttggttta tttttggttt ttttcctttc ttgttggctg
      121 gtataaacct caattaaaaa aagaatatca gatgctttgt tataataata gttggtatta
      181 tgttcttgct cgttatgatt cacggctcgt tttatctagt tcatttaaag atgacagtaa
      241 cagattctta atatttaata cagaacagtc gggattttat gaaatcaatg atgtttatgt
      301 cagaaagtaa taatcctctc ccacaaagtg aattaaatga atatataatg cgttcgaagt
      361 atatgtattg agctgtcata ctgagaaaca aaccttaggg cagcacaatt aaagccagaa
      421 acaactatgc aagaaagtta ctaaataagt gctcggaaca gtaaaacact caggggggac
      481 tcagtccgga ttatgtaatc agataaattg ccaaacatca caaatcacca gccggactga
      541 gtgatgccga gcattgcacc aataccccgt gatgaacgat gcctgatgca gaaagctccc
      601 tataaaacaa agaacaaaaa ctacgaccgg cgattcaccg tcatactgat gctgcatcgt
      661 ggtgacactg tctgtcactt tcatgttgac tgaacaactc tgctgcgaag aaggtgtacc
      721 gccggcggga tttgattgtc cggcgtctag cttccacttc cctgatggaa ctgaaagttg
      781 ttatggccgg tcagcttgcc ctgtccgcag gacatgaggt gctgtgacgg acagcaccag
      841 taacggaaag tgacaatgaa aatgaatact gcgctgatag ttgcactaat gtgtatgtgg
      901 tatgcggtac ccgctgctgc aaaagagact ttactggcta tgccacgtaa ttcaacagaa
      961 cattgctatg cggagattaa tgtgcacgga ccatatgggg tgtatttccg agtggtcccg
     1021 catccccccg ggggaaagag ctgggtcgaa tgtaacagtg actactatta ctctgataaa
     1081 ccaccgggag tacagatact cggaacccgt gcagggtgtc gggtatacgg aatctgcggg
     1141 accacctcga cactgcatgt ggctggaaga ggggttgtgt gtattaaaaa tatatgcagt
     1201 ccacgtggga tgataattca caggatacgg aaacgtcctg tagtggctgt aagcgatgag
     1261 atgtgatctt acccagcaat agtagacacg cggctaagtg agtaaactct cagtcagagg
     1321 tgactcacat gacaaaaaca gtatcaacca gcaaaaaaac acgtaaacag cattcgcctg
     1381 aattttgcag tgaagccctg aagcttgctg aacgcatcgg tgttgctgcc gcagcccgtg
     1441 aactcagcct gtatgaatct cagctctatg cgtggcgcag caaactgcag caacagatga
     1501 cctcgtctga gcgtgaaagt gaactgcccg cctgaagcgg caactggcgg agcaggcaga
     1561 ggaactggct atcctccaaa aggccgcgac atactttgcg cagcggctgg tatacgtggt
     1621 gtcagcgtcg gacaggaata agccctcgtc agcagttccg tcagcactgc gacagcgttg
     1681 tcctcgcggc ggcctttacc cggtcaaaac agcgttacgg tgcaccacgc ctgacggatg
     1741 aactgcgtgc tcagggttac cactttaacg taaaaaccgt ggcggcaagc ctgcgccgtc
     1801 aggggctgag ggcaaaggcc tcccgg
//
LOCUS       DAZOZX010000338         1809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZOZX010000338 DAZOZX010000000
VERSION     DAZOZX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.344"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            173..1198
                     /locus_tag="ACHUPT_004396"
     CDS             173..1198
                     /locus_tag="ACHUPT_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0821655.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHUPT_004397"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHUPT_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZOZX010000339         1797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZOZX010000339 DAZOZX010000000
VERSION     DAZOZX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.345"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1146)
                     /gene="mxiA"
                     /locus_tag="ACHUPT_004398"
                     /gene_synonym="virH"
     CDS             complement(<1..1146)
                     /gene="mxiA"
                     /locus_tag="ACHUPT_004398"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR0821656.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIE"
     gene            complement(1159..>1797)
                     /gene="mxiC"
                     /locus_tag="ACHUPT_004399"
     CDS             complement(1159..>1797)
                     /gene="mxiC"
                     /locus_tag="ACHUPT_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR0821657.1"
                     /translation="KIINEIISGSEKSVNAGINSAIQAKLFGNKMKLEPQLLRACYRG
                     FIMGNISTTDQYIEWLGNFGFNHRHTIVNFVEQSLIVDMDSEKPSCNAYEFGFVLSKL
                     IAIKMIRTSDVIFMKKLESSSLLKDGSLSAEQLLLTLLYIFQYPSESEQILTSVIEVS
                     RASHEDSVVYQTYLSSVNESPHDIFKSESEREIAINILRELVTSAYKKELSR"
BASE COUNT          636 a          369 c          252 g          540 t
ORIGIN      
        1 ttcaataaga ccttccatat catttgcatt tatcttattt tcggcaaata ataaaattaa
       61 aggaacggtt tctgaactaa tagcatccag attttctatc attgccaaag atgagtcatg
      121 gctattatca atatcaaatg taccagtata gccactagaa tcagactcag acagactttt
      181 ttctttttct acgacctttt tataatagaa taaagccgtc aaagtaactg ctatcaggaa
      241 aaaaacaaaa aacggaaaac ctggcaacat ccctatcgcc aaggcaagag ctgatgtaac
      301 aatgagaaca aaaggattcc caaatatctg ggacattatg ttacgtccca tattatcgct
      361 atcaccatta actcttgtaa cgataaatcc tgcacttata gaaattaaca gagcaggaat
      421 ctgagagacc aatccgtcac caatagttag tatggtataa gtagataatg caccagatag
      481 ggacattcca tgttgactca taccaacaga aatacctcct attaaattga caaaaataat
      541 aatgatgcca gcaattgcat cacctttaat gaacttcatt gctccatcaa atgaaccata
      601 caattgactc tcacgttcca gaatactgcg tctttcttta gctcctgcgg catcaataat
      661 tccagccttt aaatccgcat caatgctcat ttgtttcccc ggcataccgt caagagaaaa
      721 gcgagcagct acctccgcaa ccctttcaga tccttttgtt ataacaatga actgcacaac
      781 agttactatt gaaaaaataa caaaacctac agccagacta tcaccaatga caaactggcc
      841 aaatgtagta ataattttac ccgcatccgc atcaactaaa attagtcgac tagtacttat
      901 tgataaagcg agacgaaata atgtcgttat caacaaaacc gaagggaatg tagaaaaact
      961 caatattctt tcaatataaa atgatcccat aaaaacgaga atagcaagta caatattaag
     1021 ccctattaaa aaatcaacaa ggtatgtagg taatggtatg atcaacatag caatgatcat
     1081 aaccataagc actaatataa ttaattcagg cttagtactt acttgcttaa gaaaagactg
     1141 gatcactttt atctcctgtt atctagaaag ctctttcttg tatgcacttg tgacaagctc
     1201 tcgtagaata ttgatcgcaa tttctctttc actttcactt ttaaatatat catgaggact
     1261 ttcattaaca gaagatagat atgtttgata cactacagaa tcctcatgac tggctcgtga
     1321 tacttctata acagaagtaa gaatttgctc actttcactt ggatattgaa aaatatataa
     1381 taaagttagc aatagctgct ctgcacttaa actgccatct tttagcaagc ttgaggattc
     1441 cagtttcttc ataaaaatta cgtctgaagt tctaatcatc ttaattgcaa ttaatttaga
     1501 taacacaaaa ccaaactcat aagcattaca gctcggtttc tcagaatcca tgtctacaat
     1561 cagtgactgc tctacaaaat tcacaattgt atgtctgtga ttaaaaccaa aattaccaag
     1621 ccattctata tactgatctg ttgttgatat gttccccatg ataaaaccac gataacatgc
     1681 acgcaaaagc tgtggttcaa gtttcatttt gttgccaaat aatttagcct gtatagctga
     1741 atttattcca gcattaacac ttttttcact acctgatatt atctcattaa taatttt
//
LOCUS       DAZOZX010000340         1788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZOZX010000340 DAZOZX010000000
VERSION     DAZOZX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.346"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            292..1290
                     /locus_tag="ACHUPT_004400"
                     /pseudo
     CDS             292..1290
                     /locus_tag="ACHUPT_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1377..>1788)
                     /locus_tag="ACHUPT_004401"
     CDS             complement(1377..>1788)
                     /locus_tag="ACHUPT_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821658.1"
                     /translation="ALWKARDGSTTSLECTSTLTRNAKTDHSLYYSANLSVILKKDGS
                     GSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGNAKAKVSGLENQIKDENFRLNFL
                     DASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
BASE COUNT          473 a          372 c          366 g          577 t
ORIGIN      
        1 gtcttttact aaataatgga aaacaatcca tccctggaag acaattgtaa aaaaaaacaa
       61 cagaataaga attaatctta ggataaatat ttatttatca tggccttttg accgctaaaa
      121 aatggcactc ggcacagtaa accccgctat agttatgcta tgcatttgtc tttaatgata
      181 tctcgtcgtt atattaacag gctgaaatta tcattaatgc acatttcaat attagcaggg
      241 atacctgagg agtttaaagt gaacatatat atcgggtagc ttttcaaatt aatccctttg
      301 attataggct taatttgcat cgcgttaggt ggctttgtgc tggaaagttc agggcaacgt
      361 cacgggtcat gtgctgattt ctctggcggc catatgcctg gcattattca ctaccgcatt
      421 tattatcatt tcgcaactca cgcgcggcgt taatacgttt tacaatacat tgttccccat
      481 tattggctat gcggggtcaa ttatcaccat gatatggggt tgggcactgt tagcaggtaa
      541 tgatgtgatg gcagacgagt ttgttgccgg ccatgttatt tttggcgttg gtatgattgc
      601 cgcatgtgta tcgacggtgg cagcgtcgtc cggtcacttt ctgctcattc ccaaaaatgc
      661 agcggggagc aagagcgacg gaacaccggt acaggcttat tcttcattaa tcggtaattg
      721 cctcattgcc gttcccgttt tactcaccct gctcggtttc atttggtcta ttacgctgtt
      781 acatagtgcc gacataactc cgcattatgt cgccttgggt taaccgcaat ctgtgcctgt
      841 ctaattggcc tagtggccac aatcgtccat caaacacgta atacgttctc atccaaagag
      901 cactggctgt ggtgttattg ggttattttt ctcggctcag tcacggtgct gcaggggata
      961 tacgtcttag tcagttccga tgcaagcgcc cgactggctc ccggcattat tctcatttgc
     1021 ctcggaatga tctgttacag catattctca aaagtctggc tactggcact ggtctggaga
     1081 cgtacctgtt cgttagccaa cagaataccg atgataccgg tatttacctg tctgttttgc
     1141 ttgtttttgt catcattcct cgcggaaatc gcacaaactg agctggacta ctttattcca
     1201 tctcgggttc tggttggatt aggcgcagta tgttttacat tgttctctat tgtttcaata
     1261 ttagaggccg gttctgcgaa aaaaaattaa aatttacata atcccagcgt tattcatatc
     1321 ttaacgctgg ttttttacga acttagtgaa ataatgtaga atataaaatt ataattttat
     1381 attggagcac atgtgttaat gatcaacccc gggatactaa aaatatagac attattaaac
     1441 ttgcttagcc tcgcattgcc tttacctgtt aaagatgcat cgagaaaatt gagtctgaag
     1501 ttctcatcct ttatctgatt ttccagtccc gaaactttgg ccttagcatt acctgagatc
     1561 cgtccgttac tatctatttt gtaggtaaaa aaatacgaat gggaaaattt cctcggtgta
     1621 tcttcatcag ttaagccaac aatcgtaaaa ctaccgctac catctttttt taaaatgact
     1681 gaaaggttag cagagtagta taaagaatga tctgttttag catttcttgt taacgtcgac
     1741 gtacattcaa gagacgttgt gctaccatca cgagccttcc agagagca
//
LOCUS       DAZOZX010000341         1786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZOZX010000341 DAZOZX010000000
VERSION     DAZOZX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.347"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            311..505
                     /locus_tag="ACHUPT_004402"
     CDS             311..505
                     /locus_tag="ACHUPT_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821659.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHUPT_004403"
     CDS             866..994
                     /locus_tag="ACHUPT_004403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821660.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHUPT_004404"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHUPT_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          541 a          393 c          377 g          475 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattat
//
LOCUS       DAZOZX010000342         1760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZOZX010000342 DAZOZX010000000
VERSION     DAZOZX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.348"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..264)
                     /locus_tag="ACHUPT_004405"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="ACHUPT_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHUPT_004406"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHUPT_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR0821661.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1164..1349)
                     /locus_tag="ACHUPT_004407"
     CDS             complement(1164..1349)
                     /locus_tag="ACHUPT_004407"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0821662.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1374..1646)
                     /locus_tag="ACHUPT_004408"
     CDS             complement(1374..1646)
                     /locus_tag="ACHUPT_004408"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR0821663.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          434 c          319 g          505 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
     1501 ggttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg
     1561 aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga atgacagcgc
     1621 ggacaatgaa cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac
     1681 ttctgtcgat tgaagacatc acccgaagtt tgaattcatt cgaatacttt ttacgaggtt
     1741 cttttcgcca gtcctgtaat
//
LOCUS       DAZOZX010000343         1747 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZOZX010000343 DAZOZX010000000
VERSION     DAZOZX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.349"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUPT_004409"
     CDS             complement(<1..140)
                     /locus_tag="ACHUPT_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0821664.1"
                     /translation="MAKIDVVCHRYSETQGGIRNGHSSSGGLLYRCKQCLKTFQFDYR
                     YNG"
     gene            216..>1747
                     /gene="ospD3"
                     /locus_tag="ACHUPT_004410"
     CDS             216..>1747
                     /gene="ospD3"
                     /locus_tag="ACHUPT_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR0821665.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNG"
BASE COUNT          587 a          330 c          338 g          492 t
ORIGIN      
        1 ccgttgtagc ggtagtcgaa ctggaaggtc ttcaggcact gcttacagcg atagagcagc
       61 ccccctgaac tggaatggcc gttccggata cccccctgag tttcagagta acggtgacaa
      121 accacatcaa tctttgccat caatcatcca aagggcaaag agtacaacaa cactaagtct
      181 gcgtcacaac ccatcaatga aaggaatata tacatatgcc atcagtaaat ttaatcccat
      241 caaggaaaat atgtttgcaa aatatgataa ataaagacaa cgtctctgtt gagacaatcc
      301 agtctctatt gcactcaaaa caattgccat atttttctga caagaggagt tttttattaa
      361 atctaaattg ccaagttacc gatcactctg gaagacttat tgtctgtcga catttagctt
      421 cctactggat agcacagttt aacaaaagta gtggtcacgt ggattatcat cactttgctt
      481 ttccggatga aattaaaaat tatgtttcag tgagtgaaga agaaaaggct attaatgtgc
      541 ctgctattat ttattttgtt gaaaacggtt catggggaga tattattttt tatattttca
      601 atgaaatgat ttttcattcc gaaaaaagca gagcactaga aataagtaca tcaaatcaca
      661 atatggcatt aggcttgaag attaaagaaa ctaaaaatgg gggggatttt gtcattcagc
      721 tttatgatcc caaccataca gcaactcatt tacgagcaga gtttaacaaa tttaacttag
      781 ctaaaataaa aaaactgact gtagataatt ttcttgatga aaaacatcag aaatgttatg
      841 gtcttatatc cgacggtatg tctatatttg tggacagaca tactccaaca agcatgtcct
      901 ccataatcag atggcctgat aatttacttc accccaaagt tatttatcac gcgatgcgta
      961 tgggattgac tgagctaatc caaaaagtaa caagagtcgt acaactatct gacctttcag
     1021 acaatacgtt agaattactt ttggcagcca aaaatgacga tggtttgtca ggattgcttt
     1081 tagctttaca aaatgggcat tcagatacaa tcttagcata cggagaactc ttggaaactt
     1141 ctggacttaa ccttgataaa acggtagaac tactaactgc ggaaggaatg ggaggacgaa
     1201 tatcgggttt atcccaagca cttcaaaatg ggcatgcaga aactatcaaa acatacggaa
     1261 ggcttctcaa gaagagagca ataaatatcg aatacaataa gctgaaaaat ttgctgaccg
     1321 cttattatta tgatgaagta cacagacaga tacccggact aatgtttgct cttcaaaatg
     1381 gacatgcaga tgctatacgc gcatacggtg agctcattct tagcccccct ctcctcaact
     1441 cagaggatat tgtaaatttg ctggcctcaa ggagatatga caatgttccc ggacttctgt
     1501 tagcattgaa taatggacag gctgatgcaa tcttagctta tggtgatatc ttgaatgagg
     1561 caaaacttaa cttggataaa aaagcagagc tgttagaagc gaaagattct aatggtttat
     1621 ctggattgtt tgtagccttg cataatggat gtgtagaaac aattattgct tatgggaaaa
     1681 tacttcacac tgcagacctt actccacatc aggcatcaaa attactggca gcagaaggcc
     1741 caaatgg
//
LOCUS       DAZOZX010000344         1746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZOZX010000344 DAZOZX010000000
VERSION     DAZOZX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.350"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            453..1301
                     /locus_tag="ACHUPT_004411"
     CDS             453..1301
                     /locus_tag="ACHUPT_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0821666.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1318..1599
                     /locus_tag="ACHUPT_004412"
                     /pseudo
     CDS             1318..1599
                     /locus_tag="ACHUPT_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1635..>1746
                     /locus_tag="ACHUPT_004413"
     CDS             1635..>1746
                     /locus_tag="ACHUPT_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821667.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGK"
BASE COUNT          498 a          398 c          411 g          439 t
ORIGIN      
        1 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
       61 tgagaacttc agtaaagata ccaccaaagc ccggaagtgg tgaaataaaa ccgggcacaa
      121 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgtgaaa tttaaatggt
      181 gggagtgcgc ttccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      241 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      301 cggctatgcg cacgcggcac agttaaaagt catgttagtc cttattggtt tgggtgggaa
      361 agccaactgt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcaaca
      421 aagttcactt cggtgatgaa aggaaagaga aaatgttgaa tgtagctatt gaaaaccaga
      481 acgggtggaa ttatagtgca cctgcacctc ataaaacggg tgccgggatt gctaccccga
      541 cgatgactac tgcgcataac cgcgctcagg cggttttttt atgcgtcaag cacagccaca
      601 ttcagattat ggtggggcgt gcagggcagc cgcaaggctg gccggtttcg gtagtcaccg
      661 gttgtagcaa ccctgtgcgt ctcaccaccc atgagattgc tacctctggt ggtgagtcat
      721 ttaaactgac tatcgaggct gccatcatgg ctactatcct taccctttct caccctgacg
      781 caaccatcga aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga
      841 aaatgcacaa aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc
      901 ggcttaattt taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc
      961 aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg
     1021 cattcaagga agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata
     1081 acaccccacc tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg
     1141 acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc
     1201 tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa
     1261 ttaaaaatat gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg
     1321 aaccctatgc ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga
     1381 atcaaaaatt tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta
     1441 attccctgtg cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgct
     1501 gaacttattt acatgcacgg aaccatgatg aggaaaaaga aacgcaaata ttgtagtgaa
     1561 atctgcgccg aaaaagacca gatggcacac gaactttaat taactgacta ttcgaaactg
     1621 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgaaca
     1681 ccaattatga agccactgtt gcaactactg ataacattgt tcacgaggtt tacctggaag
     1741 gaaagc
//
LOCUS       DAZOZX010000345         1744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZOZX010000345 DAZOZX010000000
VERSION     DAZOZX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.351"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHUPT_004414"
     CDS             complement(<1..353)
                     /locus_tag="ACHUPT_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0821668.1"
                     /translation="MNRHTQIRQSVLACLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHUPT_004415"
     CDS             complement(350..940)
                     /locus_tag="ACHUPT_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0821669.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHUPT_004416"
     CDS             complement(927..1298)
                     /locus_tag="ACHUPT_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0821670.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUPT_004417"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUPT_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0821671.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          527 c          407 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcatgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZOZX010000346         1733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZOZX010000346 DAZOZX010000000
VERSION     DAZOZX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.352"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..143
                     /locus_tag="ACHUPT_004418"
     CDS             <1..143
                     /locus_tag="ACHUPT_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0821672.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHUPT_004419"
     CDS             127..486
                     /locus_tag="ACHUPT_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821673.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHUPT_004420"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHUPT_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR0821674.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHUPT_004421"
     CDS             707..1141
                     /locus_tag="ACHUPT_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821675.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1733
                     /locus_tag="ACHUPT_004422"
     CDS             1063..>1733
                     /locus_tag="ACHUPT_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0821676.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQINMQRRV
                     AWINP"
BASE COUNT          569 a          379 c          428 g          357 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataaa tatgcagcgc cgggtggcat ggataaaccc gga
//
LOCUS       DAZOZX010000347         1719 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZOZX010000347 DAZOZX010000000
VERSION     DAZOZX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.353"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..498)
                     /locus_tag="ACHUPT_004423"
     CDS             complement(<1..498)
                     /locus_tag="ACHUPT_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821677.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTI"
     gene            complement(669..1607)
                     /locus_tag="ACHUPT_004424"
                     /pseudo
     CDS             complement(669..1607)
                     /locus_tag="ACHUPT_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          640 a          346 c          245 g          488 t
ORIGIN      
        1 gatagtaaat agtaggattg tagcaataat gcctggataa taagttttcg taatttccca
       61 tgaacagcca acaagtccac aggctattac aaaaaaacag atatttgcta aaaactgagc
      121 taaccttaca tctcctaact gtaaacctaa agccattaaa agatctgcat tttgcgggaa
      181 cagaggaaaa cgcaaatatt gttcaacaac aattgcatgg tgctcaacta atgaacgagc
      241 caaaggtaag tgatacatag tatcatccca atcaccggga gctcgcaggg acgataacaa
      301 tgtaaccaca aaaatcaaaa caagaaataa aataacaaac agcgtactga actcaatatt
      361 atttattgcc agccatctaa attgctgacg tttaataatg cttaaaaccc aacacaccgt
      421 aaaaattaaa aagatagcaa tcttattaaa ttgcccgacc atgcccagaa agtaaattat
      481 caaaatattt aaagacatcc ctatgcatag gcaataaaaa ataaagaaaa tttgttaagt
      541 cctgccaccc cctccttaaa aaaagatgac aatacaatat taccaatgac aaaaaatgtc
      601 attccaaaag caaaacaata cacaacatac aaatacaacg gcaattcaat catagtaaaa
      661 tactctattt acattatcag tttatagttt ttttcagaaa gataaaataa tgccttcatt
      721 tcacgtctag ttgttgtatt tgaatgcaaa ataatgccac atacaaatgc cattattgac
      781 gataacatga tggaggcagt taaaaaggca gtcggaaatc ttctcaccaa acctgtatca
      841 atataatcaa caatgatggg caatcctaaa attatagaca aagcagcaag taaaacgccg
      901 ataatactaa aaaagtatag tggccgttca agcgagtaaa gtttaattat tgttttcaga
      961 atcttgaacc catcactcca agttgatagt ttgctcacac tcccttcaga tcgctcgcca
     1021 tattttgtat taacctcacc atacttcata cgtaattcta aagcatgaat tgtaagctca
     1081 gtttcgattt caaatcccct tgatagacat ggaaaagatt taacatagcg tctggaaaat
     1141 acacgatacc ccgacaacat atcagtaaaa ttccctttaa agatagtctg aacagtacca
     1201 gtcaacaatt tatttcctca gcgatgccct tttctgtatg tattttgatc tccattctct
     1261 tgacgacatc ctacaaccat atccaattga ttttttatga gatggttaat catttctgga
     1321 gcagaacttg catcataagt gtcatctcca tcaaccatta gataaatatc cgcatctaca
     1381 tctgagaaca tacgtcttac gacattcccc ttgcctttca gaggaacaga gtgaactgtt
     1441 gctccagcat cctctgccac ttgtgctgta ctgtcagtcg aattattatc aaatacatat
     1501 atagaagcag ttggaatatt cgttttgaaa tcatttatga cttttgctat tgccttctgc
     1561 tcattataac aaggaataat tacagcgata ttaaaagagt gatacataag cccctcatat
     1621 aaactattta gctaatgtgc aagtaattgt gtttttaaca aatcgtttac taacgaaaaa
     1681 tgaaatattt tgatgcgcaa tatcctaaaa ccaaactaa
//
LOCUS       DAZOZX010000348         1717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZOZX010000348 DAZOZX010000000
VERSION     DAZOZX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.354"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(110..577)
                     /locus_tag="ACHUPT_004425"
     CDS             complement(110..577)
                     /locus_tag="ACHUPT_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0821678.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACHUPT_004426"
     CDS             complement(643..882)
                     /locus_tag="ACHUPT_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR0821679.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACHUPT_004427"
     CDS             complement(1017..1622)
                     /locus_tag="ACHUPT_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0821680.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAZOZX010000349         1675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZOZX010000349 DAZOZX010000000
VERSION     DAZOZX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.355"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1189)
                     /gene="ospC4"
                     /locus_tag="ACHUPT_004428"
     CDS             complement(<1..1189)
                     /gene="ospC4"
                     /locus_tag="ACHUPT_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFR0821681.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNIILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFT"
BASE COUNT          506 a          286 c          289 g          594 t
ORIGIN      
        1 ttgtaaaatt aaatttagat agtaaataaa gtgccatatc agattttgca ttgcctagag
       61 aataagcaag agcttgatga gccatttttt tatcagtcac cttattgtta atttcttcat
      121 aattggatgc tttaatggca tcctccaaag atatatctct gagtcttatc tttttgaagt
      181 tatctgtgct tagcattctt ggtatatgat actcaagctg aaacacaaag tttataattc
      241 tatccagact aacaggatca atatttttgt tataacaaaa ctctctgaat ccttgatctt
      301 tactctttct tataaaatca ataagatgca acccaattcc aagcctcata tctttagtgt
      361 taaatattgg aacatcagtt ttcccctgat taccatgcac atacctgcta acttcatcca
      421 caacctcttt aaagttatct aagaaaaatg attggtgctc atggtaaaaa acaggtggaa
      481 tagcattatc aaagtgatcg gttaatgtca tatatccata tggagagtca tgatcaatga
      541 tatacgcatt ggcgccaaaa tctacagtat gatgtgtttt atttagtggg agtttttctt
      601 gatgattgga aatttcgacc ccaaaaaaaa caaaatcgtg gttagagatt tctttaatat
      661 cctcagagta tgtattctta tgtgtttgta tattttcaga ctgaatttta tcaaaggatt
      721 taatattaag aataatattt gacagattag agttactttg atgtctgaag ttaaaattag
      781 aattaataat aatagaaagt aagtgacgtt cgtctgaaga aagaggatga actttttgta
      841 ttatctcaga gatatagtgc ctggacacct tatcgagatg ctcaagtgac cgaaatgatg
      901 accgtgctcc agatggcgct tcaagagaaa gatttagtga tttgaaactg gtcccttgag
      961 aaaacatttt gctataactt tgcgctgcta tagacttcct taaaaagttt ttcacagtat
     1021 tagcacagtg atctaaatgt ttttcactta tgccagaaga agatgagttt gttacgcgta
     1081 atacattttt ttgtaatgct tttgttgctt tattcggatt cgtttttcca tcaacaagat
     1141 caatattatt ctgaacatta atatgattta ctgcttcagg tattttcata aaacatcccc
     1201 cattatgtgc gtttttatct gtggttattt agataacatt atctaactgg acactttctt
     1261 gtatgtatta tagctaaatg aacagatttt aatatgttgt atggttttca ctctgaaatc
     1321 ccctgaattt aatcacgttt cctttactga aggcaatttt aaccgtagta tcaattatta
     1381 acccttgggc ttgatatccc tctcctcttc tgggcacggg ggatgtcaga ttacttatta
     1441 cagaaaccaa aagaattgta agcgtcatct cagtgtcgtc tatttcaatg tctgatgatt
     1501 ggcgatgata gagtcctctt ataacgttaa tggactctat caatgtcaga ttctcttcgc
     1561 ccccgcagga ttcgtgcctc ttactctatg actctatgga gtttaagctt gatcttattg
     1621 aaaaatctta ccagctaggg gcctgcgtgg cccaactggc cagagagtac gggat
//
LOCUS       DAZOZX010000350         1658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZOZX010000350 DAZOZX010000000
VERSION     DAZOZX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.356"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(6..509)
                     /locus_tag="ACHUPT_004429"
     CDS             complement(6..509)
                     /locus_tag="ACHUPT_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0821682.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(554..1517)
                     /locus_tag="ACHUPT_004430"
                     /pseudo
     CDS             complement(554..1517)
                     /locus_tag="ACHUPT_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          472 a          358 c          335 g          493 t
ORIGIN      
        1 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
       61 cacaaggcat aaaacatcat taccactcca cttaatggca gaatgatata tacgtatccc
      121 atcggtattt gcatagcggc agatacctgt gatgttgcca gtgttttatc aattaatttc
      181 aggccaccag tcacaatgac cgaaccggca aaacctaaaa tgagaacgtt aatgactata
      241 ctaagaagta attgtttgcg cttattcagg gcgagagcaa ataaatcaat agagagatgt
      301 cgctgcgcgc cgacggtata tgcagcacct aataatccta cccagatcat caaaaagcgt
      361 gccagctcat cggttaatgt acttggctgg ttaagtacgt agcggctaaa tacttgccag
      421 actacgcata caaccagagc aagcattacg gcgacagaaa acgtggcaat gacgcggtct
      481 accgcaagtt tcaggcggtt aaatatcatt gtaagcatcc tggaaaatca gggccagagg
      541 ctggcccgga atattattga gcagcgttgt cgaatttctc taacaaagca gcttgttttg
      601 gatctttttt gaagtcgtca aacagaggtt gcacggcttt acggaatggc gctttatcca
      661 cttttacaat ttcacccccc atagctttcg cctgagcacg tgtgtcagcg tcaatttttt
      721 cccacaattt ttgttgataa gcttcggatt tcttcgccgc tgtttcgagg atttgttgct
      781 gttctggggt taatttattc catgttttgg tggagataac caaaaagtca ggaattgagg
      841 cgtgttcatc ttcagaaaaa actttggcaa tttcaatatg tcgagtctgc atccaggaag
      901 gcacgttatt ttctgcgcca tctactacac cttgctgcat cgcagtataa acttcgccaa
      961 atgaaattgg agtgggtgaa ccacccatca gttcaatcat tttgatggtg gtcggacttg
     1021 gctgaacacg aattttcagt cctttaagat cttctggctt tgtaatcggt tttttcgcat
     1081 agaaactgcg agtacccgct acataagctg ataaagcaaa aaagcctttg tcttttgttg
     1141 agtccataat ttctttacca acttcaccaa aaacaactta ataagaacgg caaattatag
     1201 atagcataaa tattatcaaa agactccaga tcactggcgg aacctttggt catatccagt
     1261 gcgccatttt gcaacaattc catagtttct cgcgcattac ccatctggct gctgggataa
     1321 atacggatca ccatattacc ttttgacagc tgttttacct ctttcgccag ctcttcaaaa
     1381 ctctgatgta caacatgact tctttccagg ttgtgtgcta actttaacgc cactttttct
     1441 gcggcgttta cgccagtgct aaccagagcc agcaaggatg cgcagactaa tgagcgggta
     1501 aatgacctca ttttcataaa aaaactccct gttgaaggat ggaaattggt tgatatctaa
     1561 ttgtgcttat cagtgatttg tgcgacaaat tggttaacct gtctgacaac ttgtctatgt
     1621 ttatcacaac ttgaaataaa gtcagaaatt aaatatta
//
LOCUS       DAZOZX010000351         1604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZOZX010000351 DAZOZX010000000
VERSION     DAZOZX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.357"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(335..481)
                     /locus_tag="ACHUPT_004431"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="ACHUPT_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="ACHUPT_004432"
     CDS             535..717
                     /locus_tag="ACHUPT_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0821683.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="ACHUPT_004433"
     CDS             complement(818..1147)
                     /locus_tag="ACHUPT_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0821684.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1604)
                     /locus_tag="ACHUPT_004434"
     CDS             complement(1319..>1604)
                     /locus_tag="ACHUPT_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821685.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          378 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atgg
//
LOCUS       DAZOZX010000352         1593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZOZX010000352 DAZOZX010000000
VERSION     DAZOZX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.358"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            7..657
                     /locus_tag="ACHUPT_004435"
     CDS             7..657
                     /locus_tag="ACHUPT_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821686.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHUPT_004436"
     CDS             629..1063
                     /locus_tag="ACHUPT_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0821687.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          450 a          325 c          377 g          441 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atg
//
LOCUS       DAZOZX010000353         1581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZOZX010000353 DAZOZX010000000
VERSION     DAZOZX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.359"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUPT_004437"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUPT_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR0821688.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHUPT_004438"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHUPT_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR0821689.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZOZX010000354         1580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZOZX010000354 DAZOZX010000000
VERSION     DAZOZX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.360"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            13..1215
                     /gene="virA"
                     /locus_tag="ACHUPT_004439"
     CDS             13..1215
                     /gene="virA"
                     /locus_tag="ACHUPT_004439"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR0821690.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            1394..>1580
                     /locus_tag="ACHUPT_004440"
     CDS             1394..>1580
                     /locus_tag="ACHUPT_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0821691.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          557 a          327 c          270 g          426 t
ORIGIN      
        1 aggagaaatc aaatgcagac atcaaacata actaaccatg aaagaaatga ctcatcatgg
       61 atgtcaactg tcaaatcaac tacagaagta agttggaata aattgagttt ttgtgacatt
      121 ttactgaaaa taataacttt cggcatatat tctccacacg aaactctagc cgaaaaacat
      181 agcgaaaaaa aactaatgga tagtttctca ccatcactat cacaagataa aatggacgga
      241 gagtttgccc acgcaaacat agatggcatt tcaatacggc tatgccttaa caaaggcata
      301 tgttcggttt tttatcttga tggagataaa atacaatcca ctcaactttc atcaaaagaa
      361 tataataact tgttaagttc actccccccc aaacaattca acttaggaaa agtacatact
      421 attacagctc cggtatcagg gaattttaaa actcacaagc ctgcaccaga agttattgaa
      481 acagccatta attgctgtac atcgataata ccaaacgatg attacttcca cgttaaagac
      541 actgatttta atagtgtttg gcatgatatt tacagagata ttcgagcatc tgacagcaat
      601 agtacaaaaa tatatttcaa caatattgaa ataccactaa aattaattgc agatctaatt
      661 aatgagttag gaattaatga attcattgat agcaaaaaag agcttcaaat gctaagttac
      721 aaccaagtaa ataaaataat caattcaaac ttcccccaac aagacctatg ttttcaaaca
      781 gaaaaacttt tgtttacttc actctttcaa gaccctgcat ttatatctgc actaacatct
      841 gcattctggc aatctcttca catcacgtct tcctctgtgg aacacatata tgcccaaatt
      901 atgtccgaaa acatagaaaa caggcttaat ttcatgcctg aacaacgagt tattaacaat
      961 tgtggacata ttataaaaat caacgcagtt gtccctaaaa acgacactgc aatctctgcc
     1021 tctgggggga gggcttacga agttagctca tcaatattac catcgcacat aacatgtaat
     1081 ggtgttggta ttaataaaat agaaacaagc tatctcgttc atgctggaac acttccaagc
     1141 agtgaagggc ttcgcaatgc tatccctcca gaaagtcgtc aagtatcatt tgccatcata
     1201 tctcctgatg tttaagccaa tattatattg gctgccagac tgtaaaaaaa cgttcttcag
     1261 aacagctcta taaactatta aaccaaagct aaaaggatgg ggaccaatag aatctcagcc
     1321 catccttttt atatcattat gaaccgcccc ggaaatcctg gagactaaac tccctgagaa
     1381 agaggtaaac aggatgacta aaaatacacg tttttcccct gaagtccgtc aacgggcagt
     1441 tcgtatggtt ctggaaagtc agggcgaata tgactcacaa tgggcagcga tttgttccat
     1501 tgccccaaag attggctgta tactggaaac tctgcgtgtc tgggtacgcc agcatgagcg
     1561 ggataccggg ggcggtgagg
//
LOCUS       DAZOZX010000355         1551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZOZX010000355 DAZOZX010000000
VERSION     DAZOZX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.361"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..294
                     /locus_tag="ACHUPT_004441"
     CDS             <1..294
                     /locus_tag="ACHUPT_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR0821692.1"
                     /translation="SAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFE
                     SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            300..1397
                     /locus_tag="ACHUPT_004442"
     CDS             300..1397
                     /locus_tag="ACHUPT_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0821693.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          480 a          351 c          383 g          337 t
ORIGIN      
        1 agcgcttatc cgatttttga tcgtattgaa gaaatggcat gggctcgtca ctaccagcag
       61 atcgttcgtg aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag
      121 cacctgtttg aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca
      181 atcagccacg catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg
      241 tacatggctg aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta
      301 tggcgctttt tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt
      361 ttgctggcga cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta
      421 tgcggcaacg gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag
      481 gctctgactg ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa
      541 cccgctcgtt tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa
      601 tgatcatcga ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc
      661 gaaaccgtaa acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac
      721 gctttaccga tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat
      781 atgagtatga ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa
      841 aaatgaaagc agaaacagaa accggttatg aaccctccat ccggatccag atggagaagc
      901 aaatgaacat agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca
      961 cccgtattga tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg
     1021 agtctcttaa cctgggaggt gaacaccccg gcatcgatac ctcacgggat aacagtgaat
     1081 tatttgccaa cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgcac
     1141 ttgatgaaat tatcgagttg ctcaacaagc accatggggg caccagtaac gacgccaaac
     1201 gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa agaatcaaag
     1261 gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa ctggaaggcg
     1321 ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca ggatacgatg
     1381 agaatattcc ggtgtaaaac caagtgcatt ttgggaatga accgccccgg gaatcctgga
     1441 gactaaactt cctgagaaag aggtaaacag gatgactaaa aatactcgtt tttcccccga
     1501 agtccgtcaa cgggcagtcc gtatggttct ggaaagtcag ggcgaatatg a
//
LOCUS       DAZOZX010000356         1497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZOZX010000356 DAZOZX010000000
VERSION     DAZOZX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.362"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            117..521
                     /gene="stpA"
                     /locus_tag="ACHUPT_004443"
     CDS             117..521
                     /gene="stpA"
                     /locus_tag="ACHUPT_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0821694.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(568..1092)
                     /gene="ygaP"
                     /locus_tag="ACHUPT_004444"
     CDS             complement(568..1092)
                     /gene="ygaP"
                     /locus_tag="ACHUPT_004444"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0821695.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1102..1401)
                     /locus_tag="ACHUPT_004445"
     CDS             complement(1102..1401)
                     /locus_tag="ACHUPT_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0821696.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          413 a          344 c          351 g          389 t
ORIGIN      
        1 aaataatctc gcgcaggact gtaaatagat taaattttgt ggaaatataa taagtgatcg
       61 cttacactac gcgacgaaat acttttttgt tttggcgtta aaaggttttc tttattatgt
      121 ccgtaatgtt acaaagttta aataacattc gcaccctccg tgcgatggct cgcgaattct
      181 ccattgacgt tcttgaagaa atgctcgaaa aattcagggt tgtcactaaa gaaagacgtg
      241 aagaagaaga acagcagcag cgtgaactgg ctgagcgtca ggaaaaaatt agcacctggc
      301 tggagctgat gaaagctgac ggaattaacc cggaagagtt attgggtaat agctctgctg
      361 ctgcaccacg tgctggtaaa aaacgccagc cgcgtccggc gaaatataaa ttcactgatg
      421 ttaacggtga aactaaaacc tggaccggtc agggccgtac accgaagcca attgctcagg
      481 cgctggcaga aggtaaatct ctcgacgatt tcctgatcta attactgagg ccggatgtcg
      541 ctgctagggc gcgtccggcg tcgcttctca agctcgttgg ttccacggca tcttatctaa
      601 caaccttgcc attccacaaa aaccgctgat tcctgcaaac agtaacccgg caccaacaaa
      661 gccacttaat aagaagaaac cgctatttac ggtataaccc agtacaacgc cgtttaatat
      721 taaaccaccc gcagcgatct gcacctggcg cattaacggc aagggttgag atttatttac
      781 cgctactggc aatccagctc ttttccagcc atcaatccca tcttcgagta aaaatatttc
      841 tgcgggggcg gcaatcgctg ctaatttatc ggcgttatta ctggtgcgtt tacctgcctg
      901 gcagtggaat ataatttgct cgcgacgtaa tttaggtgga agacctgact gttccagcac
      961 ggataatgga gccagatctg cttcaggaat atgttcacga agatattcat cagcgtcacg
     1021 aatatcgatt aactttgcgc cgcgtgcgat taattcttgt gcatcatgcg gcgaaatggt
     1081 tgtcaaagcc atagtgactc cttacggaca atagacattt ttcagggtgg cgataatggc
     1141 atttaccgcc tcatttttaa tggaatatag aatgcgttgg gcatcccgtt ggctgttgat
     1201 aagcccttcg tcccgcatac gagcgagatg ctgtgatgtc gcagaggcgc tcagtccggt
     1261 aatgcgcgtc agctctcccg cgctggtgcc gggggaaccg ctaagcatgc acagaatcag
     1321 caaccgttta gggtggctca ttgctttcaa taaggccgct gcctgttcgg tactggcctg
     1381 taattgcgcg agttcagtca tatttattta agtattttct aaattaagta aactctaaac
     1441 taaaaatgca acatatacca gcctcagcag cgtaaatgag agtaaaagcg taagctg
//
LOCUS       DAZOZX010000357         1476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZOZX010000357 DAZOZX010000000
VERSION     DAZOZX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.363"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            844..1446
                     /gene="fimB"
                     /locus_tag="ACHUPT_004446"
                     /pseudo
     CDS             844..1446
                     /gene="fimB"
                     /locus_tag="ACHUPT_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          441 a          253 c          329 g          453 t
ORIGIN      
        1 ttagaatcta aacaaagaaa gctttgcaag ctaactcagt gagagtggtg aaaataaggg
       61 tttactcgcc atcaggctga gcataattct catcatgaaa tatgtttctt ggtttgtggc
      121 ttgtaactgg tcactcctga tgtcgatctg gagaggcttg ttgatgttgg tgttttcagg
      181 atgatgtttc acttagtttg tttgccgtat cgcccggcga atagctgtga ttgaggaagg
      241 cttaagtcgt agtgaccaaa gctatattta ccaacggatg tagatgaaaa agcgtcgcct
      301 gcgttccccc atatctccag gataaaaagg aatgtaacaa tctcatggcc taagctgacg
      361 aaccaggcag gaataatcgc tagggatcta agaattagca tgataatagc cactaagaaa
      421 ttactgcgtt ccatgaaata gccattttgt ggcaaatgca gttaactaat aatgtcatat
      481 ttgagacggc tagtttaaca aatattaaat tttgctgaat tttttatgtt gattttactt
      541 gttacagaac atatcacatg atatatagat aagattagtt gcattaatga tgagggttat
      601 tattagattc gtatcagatt gataaatata taaaggtaca tagcatgcaa gagcatggcg
      661 tttgtatggc aaagttatta taagtaacta tcgctactcc atttaagttc gctcagaaga
      721 actggtccac ttacgtaagt tattaagcaa acgttcgctt ttataaacat aatcaggata
      781 aaaatgttgg attattgcta acccagcaca gctagtgcgc gtctgtaatt ataagggaaa
      841 acgatgagga ataaggctga taacaaaaaa aggaacttcc ttacccatag tgaaatcgag
      901 tcactcctta aagcagcaaa taccgggcct catgcaacac gtaattattg tctgattttg
      961 ctttgtttta ttcatggttt ccgggcgagt gaaatttgtc gattgaggat ttcggatatt
     1021 gatcttaaag caaagtgtat atatatccat cgattaaaaa aaggcttttc aacaacgcac
     1081 ccgctattga acaaagaagt tcaggcttta aaaaactggt tgagtatccg tacttcttat
     1141 ccgcatgctg agagcgagtg ggtattttta tcacgtaagg ggaatccgct ttctcggcaa
     1201 cagttttacc atattatctc gacttctggt ggtaatgccg ggttgtcact ggagattcat
     1261 ccgcacatgt tacgctattc gtgtggtttt gctttggcga atatgggaat agatacgcga
     1321 cttatctagg attatcttgg gcatcgcaat attcgtcata ctgtctggta taccgccagc
     1381 aatgcagggc gtttttacgg catctgggat agagccagag gacgacagcg tcatgctgtt
     1441 ttatagagat agttgagata ccagggatgg tgttaa
//
LOCUS       DAZOZX010000358         1460 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZOZX010000358 DAZOZX010000000
VERSION     DAZOZX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.364"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..246)
                     /locus_tag="ACHUPT_004447"
     CDS             complement(<1..246)
                     /locus_tag="ACHUPT_004447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821697.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEK"
     gene            698..982
                     /locus_tag="ACHUPT_004448"
     CDS             698..982
                     /locus_tag="ACHUPT_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR0821698.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            982..1257
                     /locus_tag="ACHUPT_004449"
     CDS             982..1257
                     /locus_tag="ACHUPT_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0821699.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          397 a          366 c          360 g          337 t
ORIGIN      
        1 tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg caaaaacagc
       61 agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa caagtcccat
      121 tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca gccctaacag
      181 aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca cataaacagg
      241 ttccatacca gtcctgaccg catcagaacg ccagtaaata aaatctcgta gtccaccggc
      301 atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact ggtgtgagcg
      361 gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg cccgctaacg
      421 cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc gcgcacagag
      481 gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac tggtgatatt
      541 cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc tacatgtaac
      601 caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg tctgaaagta
      661 atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa acaataccgc
      721 acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata aagtcgacca
      781 gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc tttctgcatg
      841 gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg atgtgacatc
      901 cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca gtactgacca
      961 tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact ttctgatttg
     1021 gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag aacggtgcag
     1081 tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga acagttgttt
     1141 cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat ccgttacgaa
     1201 cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa caggtagatt
     1261 gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat tgttatcagg
     1321 ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt acgatgaaat
     1381 aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc aaataaccca
     1441 tcagaaacgt tattgccaga
//
LOCUS       DAZOZX010000359         1434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZOZX010000359 DAZOZX010000000
VERSION     DAZOZX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.365"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            431..600
                     /locus_tag="ACHUPT_004450"
                     /pseudo
     CDS             431..600
                     /locus_tag="ACHUPT_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1434
                     /locus_tag="ACHUPT_004451"
     CDS             746..>1434
                     /locus_tag="ACHUPT_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0821700.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          311 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggt
//
LOCUS       DAZOZX010000360         1424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZOZX010000360 DAZOZX010000000
VERSION     DAZOZX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.366"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..950
                     /locus_tag="ACHUPT_004452"
                     /pseudo
     CDS             1..950
                     /locus_tag="ACHUPT_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          336 a          373 c          404 g          311 t
ORIGIN      
        1 ccctgtatcg ccagcagcac attttctccc gaatgggtgt ggagctcccc gtcagtacaa
       61 tggcggatat ggtcggtgtt gccggagctg cactggcccc gctggcgaaa ctgctacgcc
      121 atgagcttct gacacgggat gttattcatg cagatgaaac gtccctgagg ctccttgata
      181 cccggaaagg cggtaaatcc tgctccggct ggctgtgtgc ttacgtcagc ggtgaaagaa
      241 gcggtccgcc ggtcgtctgt tttgactcgc agaccggacg ggccctcaga taccctgaga
      301 cctggctgca gtgctggtgt ggcagtacgc tggtgagtga cggatacagc gtgtacaaat
      361 ctctggcgga taatcacccc ggcatcacat cagcctgttg ctggagtcac gcagggcgcg
      421 gcttcgcaaa tctgtataag gccagcaggg agccccgtgc aggcgtggag ctgagaaaaa
      481 tcgcgggtct ttaccgcatt gagaaactca tccgtgagcg ccctgtggaa aaaatacggc
      541 agtggcgata acggtactcc cggccgatag tgaatgacct gtttgcctgg cttgaagagc
      601 aggagccgtg ctgtccgccc gacgggccat taaataaggc gattaactat attctgaacc
      661 ggcgcgatga actgagctgc ttcctgggtg acggagctgt gcccctggac aacaatatct
      721 gcgagcgtgc cattcgcccg gttgttatgg gacggaaagc gtggctgttc gccggttcac
      781 tgatggccgg aaaccgggcg gcacagataa tgagccttct ggaaacagca aaacgtaatg
      841 gtctggagcc acatgcctgg ctgacggacg tcctgacgcg tctgccggaa tggccggagg
      901 agcgactggc agagttgctg cctcttgagg gatttacctt caccgggtga gtgatacctg
      961 ccgtcaggtg ttcgtgaacc gggctataac ctgcagtcgg ttgttgaacg cctgacaaca
     1021 ggaaatcagc caggccagca accacgcgga ctgacagaga atcagaacct gaatcagcct
     1081 gaaatcatac ccggggcagt acttcatcac cagtcaccaa tacccgtgtc agtacttcgc
     1141 ggcctgacgc gatcacccga ctcagtactt ctgagcccgc ctccggtacc attctctgtg
     1201 ggcccgtaca caacaacaga aaagggtgcc cggcccgggg cgcaaccgta ttacacttcc
     1261 ggaaaatcgt tatacgcaga aaaatcggat cacgctgtgc ctgctttcat gtaatttact
     1321 tacggtgtta taagtatgct aattatagta taaaaaaatc gcagcagaaa aaattgtatt
     1381 aaaaacttat agcgaattaa tggtgtactt tttatggata ttga
//
LOCUS       DAZOZX010000361         1404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZOZX010000361 DAZOZX010000000
VERSION     DAZOZX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.367"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..167
                     /locus_tag="ACHUPT_004453"
     CDS             <1..167
                     /locus_tag="ACHUPT_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR0821701.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHUPT_004454"
     CDS             152..790
                     /locus_tag="ACHUPT_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR0821702.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="ACHUPT_004455"
     CDS             1018..>1404
                     /locus_tag="ACHUPT_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821703.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAZOZX010000362         1399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZOZX010000362 DAZOZX010000000
VERSION     DAZOZX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.368"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..490)
                     /locus_tag="ACHUPT_004456"
                     /pseudo
     CDS             complement(1..490)
                     /locus_tag="ACHUPT_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(512..1052)
                     /gene="yhaB"
                     /locus_tag="ACHUPT_004457"
                     /pseudo
     CDS             complement(512..1052)
                     /gene="yhaB"
                     /locus_tag="ACHUPT_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            complement(1308..>1399)
                     /locus_tag="ACHUPT_004458"
     CDS             complement(1308..>1399)
                     /locus_tag="ACHUPT_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR0821704.1"
                     /translation="TGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          431 a          251 c          261 g          456 t
ORIGIN      
        1 ctaaatcgac attacgtaag tccagagagg atagatcaac tatatctcca gtcccacaac
       61 taaagattac gttcaacctc ctgatttcat tgtaaatatc ttttaatggg caattaacat
      121 tgatattgcc tgtagatatt tcctagtttt ttaacggttc attaatggta agttcgcagg
      181 tattgtccga tcgttgaata atttgggtgt tataacctaa aaaatccaga cgaagagacg
      241 agtcattata aagaattaca ttttgtaagt acgctaattc tttttgaaaa tcaccagtga
      301 taatgcattt tccacttggt tgtaacgcgt taagtaaagt gaccatttga atagaggtct
      361 gttttagttt ttctatacct ggtgctagtg cttcgaggtt atttcctatg gggctttcag
      421 gaaggtcatt catttttcga cggactaaat cactactaat atcattacca attgatgaaa
      481 cagggaacat tattttatcc tttataaata atcaataaaa atcattatta aaatgatcgg
      541 gtgaaaaaaa gagaatttgc caacaatctg aagtaatgca ggttttatta aagtgcctag
      601 ggggtgttaa gatggcttct ttttgggtga actttgcgca atacttagaa atcgtctcct
      661 taatacattc cagctctttt ttattgacgg ctgagcaagt acattgttca ccaaaaggtg
      721 caaaagtgcc tacatctaat gtttgtatga tataatcatc atgttctcct gtagaaatat
      781 ctatgatgaa tggactcata ttttccggtt ggattcggaa tcatgacaga aggaacatcg
      841 ccttgataga aattcaatgc agtaattgca gataatactc tccggagttc attacttata
      901 ctgtaaattt tattaacgtc attggtggag acaatttctg caaactttct ttttaccgtc
      961 caataatcaa tatttctatt gtgattgtta accactgcgt gcattggcgg gattgaaggg
     1021 tgaaaaatat gtgcaattgg gaagcctttc attgatgcct gccttgtcgt catgacaaac
     1081 aataatagat gcttattttc ctcgcacgac acttacgcac tatcaaaaaa cgctcattta
     1141 cccggcgtta cagtaaacga agcaacattc tgtgtcctac gctgatgtaa agcacagtga
     1201 aaatccccct tgttgacttc gtctgacctt atcgcggcga ccagcaatta ttcgttttgt
     1261 gatcctcaac acaaaatgct attcgtcgcc tatgagggga gaagaattta tggaaaacga
     1321 ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt tgacgttaat caattgttcg
     1381 agatgatcaa tacctgtat
//
LOCUS       DAZOZX010000363         1359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZOZX010000363 DAZOZX010000000
VERSION     DAZOZX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.369"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..659)
                     /locus_tag="ACHUPT_004459"
                     /pseudo
     CDS             complement(1..659)
                     /locus_tag="ACHUPT_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            667..1093
                     /gene="tnpA"
                     /locus_tag="ACHUPT_004460"
                     /pseudo
     CDS             667..1093
                     /gene="tnpA"
                     /locus_tag="ACHUPT_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          352 a          343 c          304 g          360 t
ORIGIN      
        1 cggcttaact gtcgctgaag tctcgccagc gtcttctggt ttttctgaaa actgtttaca
       61 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
      121 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
      181 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
      241 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgagcg
      301 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
      361 aacgccgagc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
      421 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
      481 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
      541 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
      601 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
      661 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
      721 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
      781 gacgcgacag aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa
      841 ctggttgaaa tggatggcga accagatcac gtccatctgt taattaacta tccccccaaa
      901 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
      961 acgggatcga ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
     1021 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
     1081 acagcaaaca cctggtcaga tgaaaaaacg agccttatat ccccgccctg aaggacgggc
     1141 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact
     1201 atgccccgta catgctccat cacatcctca acatcaaagt attagagcct tgcaaaccat
     1261 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
     1321 cgctatattg cgtattaaat aaaaccacct caattacat
//
LOCUS       DAZOZX010000364         1359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZOZX010000364 DAZOZX010000000
VERSION     DAZOZX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.370"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            134..853
                     /locus_tag="ACHUPT_004461"
     CDS             134..853
                     /locus_tag="ACHUPT_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR0821705.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          409 a          255 c          262 g          433 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgttcggag aggaccctta cgcttacata cattacatat
       61 aatgtatcgt tttataaaag atgataaaca atcaatataa gataatatat ctattttata
      121 gaggacgttt tctatgccca taaaaaagcc ctgtctaaaa ctaaatttag attctttgaa
      181 tgttgtgagg tctgaaatac cgcagatgct ttctgcgaat gaaagattga aaaataactt
      241 caatatattg tacaaccaaa tacggcaata cccagcgtac tatttcaaag tagcatcgaa
      301 cgtgccaact tactctgata tatgtcaatc tttttctgtc atgtaccaag gattccagat
      361 tgtgaaccat agtggagatg tgtttattca tgcatgtcgt gaaaatcctc agagtaaggg
      421 ggattttgtt ggggacaagt ttcatattag tatagctagg gagcaagttc ctctggcatt
      481 ccaaattttg tctggtttat tattttcaga ggacagtcct atagataaat ggaagataac
      541 tgatatgaat cgagtttctc agcaatctcg tgtggggata ggagctcagt ttacgctata
      601 tgtaaaatca gatcaggaat gctcgcaata tagtgcttta ttacttcata aaatacgaca
      661 atttataatg tgtctcgagt ctaatctatt aagaagcaaa atcgctcccg gggagtatcc
      721 ggcgtcagat gttagacctg aagactggaa atatgtcagc tatcgtaatg aactacgaag
      781 cgatcgagat ggaagtgaaa ggcaagagca aatgttacga gaggaaccat tttatcgttt
      841 gatgatagag tagagaagat gccagctaca ccaccttgta gcttgaaaac tggttagaat
      901 aactacgatt agatttggca ctgcgtatgc agcctgattc ctgcccgtga cagagcattc
      961 ggctcataaa ctgctgagct actcatctga aatccgaaca gttgtccacc atttcagcgt
     1021 gcatctcctg tttgagtcgt ttgattgctt ttcgggatat taacctgtcg gattttgcat
     1081 tagttaatgc tcccatcagg ccttcatctt ttccagtgcc tattttcctg ttctcctttt
     1141 gcacaatata atcagatcat ctgtatcgtt gacgagctcg ctatcttata ggagcattct
     1201 gaataagcta cctccttgta aggaggcttt tactattagt aactgcaatc taatagtatt
     1261 ataacatctc tatggttgtc ttctatgccg ttaaataaaa cattttcatc tagtatattt
     1321 tcaacaaaaa attcgttatc aacagatatg tctgttaac
//
LOCUS       DAZOZX010000365         1353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZOZX010000365 DAZOZX010000000
VERSION     DAZOZX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.373"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..226)
                     /locus_tag="ACHUPT_004462"
     CDS             complement(<1..226)
                     /locus_tag="ACHUPT_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0821706.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGDDGGLTTAERQRLKE"
     gene            303..845
                     /locus_tag="ACHUPT_004463"
     CDS             303..845
                     /locus_tag="ACHUPT_004463"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR0821707.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            1072..>1353
                     /locus_tag="ACHUPT_004464"
     CDS             1072..>1353
                     /locus_tag="ACHUPT_004464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR0821708.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          373 a          284 c          329 g          367 t
ORIGIN      
        1 gctctttcag acgctgacgt tcagcggtgg tgagccctcc atcatcgccc ccggtatccc
       61 gctcatgctg gcgaacccag acacgcagag tctccggcgt acagccaatc tttggggcaa
      121 tggaacaaat tgtcgcccat tgtgagtcat attcgccctg actttccaga accatacgga
      181 ctgcccgttg acggacttcg ggggaaaaac gagtattttt agtcatcctg tttacctctt
      241 tctcaggaag tttagtctcc aggattcccg gggcggttca ggataacacc aaaggcgatg
      301 ccatgattgg cgtcaagcag cctttaccaa aagcagtttt aagaactcag catgacaaaa
      361 ataaagaagc aataagtatc ctggattttg gtgttattga tgatggtgtg acagataatt
      421 accaggcaat acaaaatgct atagatgccg ttgcttcact accctccggc ggggagctgt
      481 ttatccctgc gagcaaccaa gcggtggggt atattgttgg atccactttg cttattcctg
      541 gcggtgttaa catcagagga gttggtaagg catcgcaact ccgagcaaaa agcggactta
      601 caggatctgt gttaaggctg tcttatgatt cagacactat cggccgttat ctgagaaata
      661 tacgagtaac tggtaataac acctgcaatg gtattgacac aaacattaca gcagaagact
      721 ctgtcatcag acaggtttat ggctgggtat ttgataatgt aatggtgaat gaagttgaaa
      781 ccgcattatt taatgcaagg gctctggcac tcaaaattta tagcatgtca ggctggaacc
      841 tgtagagtcg gtcttcactt tttaggccag tgcgtaagtg ttagtgtcag ctcctgccat
      901 ttcagcagag gaaattattc tgctgatgaa agctttggca tcaggattca gcctcaaacg
      961 tatgcgtggt cgtcagaggc agttaggtca gaagcaataa ttttagacag tgagaccatg
     1021 tgcattggtt ttaaaaatgc cgtctatgtt catgattgcc ttgatttgca tatggaacaa
     1081 ctggatttag attattgcgg ctcaacaggc gtggttatag agaatgtaaa cggaggattt
     1141 tctttctcaa actcatggat agcagcagat gccgatggca ctgaacaatt tacggggata
     1201 tattttagaa cgccggcctc aacgcagtca cataaaattg tcagtggtgt tcatatcaac
     1261 actgcaaaca aaaacacggc tgcaaacaat caaagtatag cgatagaaca gtcggcgatc
     1321 ttcgtctttg taagtggttg tacgttaact ggt
//
LOCUS       DAZOZX010000366         1345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZOZX010000366 DAZOZX010000000
VERSION     DAZOZX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.374"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPT_004465"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPT_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUPT_004466"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUPT_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0821709.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          370 a          310 c          284 g          381 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atata
//
LOCUS       DAZOZX010000367         1337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZOZX010000367 DAZOZX010000000
VERSION     DAZOZX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.375"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(208..363)
                     /gene="hokD"
                     /locus_tag="ACHUPT_004467"
     CDS             complement(208..363)
                     /gene="hokD"
                     /locus_tag="ACHUPT_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0821710.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(591..1256)
                     /locus_tag="ACHUPT_004468"
     CDS             complement(591..1256)
                     /locus_tag="ACHUPT_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0821711.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          350 t
ORIGIN      
        1 tccatccctc ttctccctgc gcgctactta agtgagtcga ttctatctgc gaaggtgcgc
       61 gaatcaaatt caccggaaat aaacaacaaa aaacccgccg aaacgctgag agatcacagc
      121 gatgggcggg ttaagtgcgg gtgcgttgag gatgcctgac acatcagagg tgggcgggga
      181 tgggatcagc tccccacccg gtcactctta cttcctggat tcgtagtcta cgaagacagc
      241 gacctccgtc tggccggttc ggattcgtac ctcacagagg tctttcctcg ttaccagtgc
      301 cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt
      361 catagcctgc ttctccttgc ctttcggcat gtaagaggct aacctacatg tgcaaagcat
      421 gaaattggcc tcagattaat gttaagcgtc ttgccggacg cgtaatgtta actggggctt
      481 ttctctatct gccttttggt gttcatgcct gaagcagata gcctcaagca cccgcagtca
      541 ttctacttac ctcgccaata tgaaatcaat cagaaaggtg ccccataaaa tcactccttc
      601 tcttctttac cgtagtggag ttgaccaatt ttgataagag ggcgtccctg agatttgcgg
      661 tgtagattgg tatcgcgcag agaatacaca cagccacaat attcctgctg atagaatttt
      721 tcgcgcttgc tgatttcaat catacgggac gagccgccct gcttgcgcca gttataatcc
      781 cagtacacca tgcccggata atgcgcgacg gctcgccgcc cacagtcgtt aacctgctgc
      841 atatttttcc agcgtgaaat gcccagtgaa ctgctgatca cactgaaacc attttcagcg
      901 gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca tggtgcaacg gatccctcgt
      961 tcgggctccc attccattcc tttggctcgt tcaaaccagt tgtctgtgtc gtaatcagca
     1021 tcaataaacg gcacgccgtg ttgttcagca aagcgaatat tctcatcctt acgaattaaa
     1081 tactctttct gaggatgaat gttcgggttg tagaaaaaga tggtgtagtc gatttccgag
     1141 gcctgaagcg cctccatcac ctcaccggaa catggagcac agcaagagtg cagtagtagt
     1201 ttgtttgccc cgtttgggag ctccaatttg ggccgtttga aatcagcaac tgtcataaat
     1261 atgtttattg aggtcataaa aatatcaaag agtgtagcat tagaacgggg ctatcggaaa
     1321 cagatgtgtg actgctc
//
LOCUS       DAZOZX010000368         1334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZOZX010000368 DAZOZX010000000
VERSION     DAZOZX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.376"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(3..485)
                     /locus_tag="ACHUPT_004469"
     CDS             complement(3..485)
                     /locus_tag="ACHUPT_004469"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0821712.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="ACHUPT_004470"
     CDS             complement(476..778)
                     /locus_tag="ACHUPT_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0821713.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="ACHUPT_004471"
     CDS             1050..1322
                     /locus_tag="ACHUPT_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821714.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          392 a          334 c          284 g          324 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccg
//
LOCUS       DAZOZX010000369         1333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZOZX010000369 DAZOZX010000000
VERSION     DAZOZX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.377"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..283
                     /locus_tag="ACHUPT_004472"
     CDS             <1..283
                     /locus_tag="ACHUPT_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFR0821715.1"
                     /translation="ELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEI
                     STFSGMNRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            283..678
                     /locus_tag="ACHUPT_004473"
     CDS             283..678
                     /locus_tag="ACHUPT_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0821716.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            861..1241
                     /locus_tag="ACHUPT_004474"
     CDS             861..1241
                     /locus_tag="ACHUPT_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR0821717.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT          472 a          259 c          259 g          343 t
ORIGIN      
        1 tgagttaata aaaaacagac acgataatga atatctccag acattaataa atgatgtagc
       61 tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg cagaaagcat
      121 caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg gcggaggagc
      181 cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga acaaggtgga
      241 tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag cagtgatcca
      301 aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca ggacaacctg
      361 acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta cagacaatcc
      421 cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct aatcagcgtt
      481 tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca aacaacagga
      541 tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc atcttcagaa
      601 aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag acgcaatctt
      661 tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc tcttctttta
      721 tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa tgcaacagac
      781 aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca ttattgtcag
      841 tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt gatgatgata
      901 taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact caggctattg
      961 cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc acctcaattg
     1021 taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa gctcttgcag
     1081 tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa gcaaagctaa
     1141 tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa ataggtcttc
     1201 tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag ttatagtaac
     1261 tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat gaggcatgga
     1321 ccatcatcaa gcc
//
LOCUS       DAZOZX010000370         1330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZOZX010000370 DAZOZX010000000
VERSION     DAZOZX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.378"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(41..100)
                     /locus_tag="ACHUPT_004475"
     CDS             complement(41..100)
                     /locus_tag="ACHUPT_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0821718.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHUPT_004476"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHUPT_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZOZX010000371         1310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZOZX010000371 DAZOZX010000000
VERSION     DAZOZX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.379"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUPT_004477"
     CDS             <1..347
                     /locus_tag="ACHUPT_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0821719.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
     gene            344..439
                     /locus_tag="ACHUPT_004478"
                     /pseudo
     CDS             344..439
                     /locus_tag="ACHUPT_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            702..950
                     /locus_tag="ACHUPT_004479"
     CDS             702..950
                     /locus_tag="ACHUPT_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0821720.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          391 a          317 c          283 g          319 t
ORIGIN      
        1 cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg
       61 acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt
      121 tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg
      181 gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag
      241 acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc
      301 gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct
      361 accgcaatgg tcacatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
      421 ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg cccaagagca
      481 tgtgttttct ttagaataat caaagcgttg gattgccatg tcatatgaaa atcggcaaag
      541 ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc
      601 cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat ccatacagtt
      661 aaaaataata ctgtataaaa acacagtgca gagggatttt tatgcgtttt gaaatctgca
      721 tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta aaggaagaat
      781 taacccgacg catcagtaaa cgttatgacg atgtagaggt gatcgtaaaa gccaccagca
      841 acgatggcct ttctgttacg cgcaccgccg ataaagattc agctaaaact tttgttcagg
      901 aaactctgaa agatacctgg gagtctgctg acgagtggtt tgttcactaa ttagcacgta
      961 aaatcagtaa cggctggaaa tcattcaata ctcgcactat cggaagttca ccagccagcc
     1021 gcagcacgtt cttgcatacg acgtgtctgc ggtctcaact ccgcccgccc ccgccaatca
     1081 tgattgggca gtaccagtac agcactagca aagacaggaa attagcagtc tcagcaaaac
     1141 accgaccaga cggtgagaag acaaaaagat acgcaaagat gccgcggttc cagaagaata
     1201 aaaaaccgct aatggacagc ctccttaaca cagtttttta tatacatgat tcactgatac
     1261 cagtggcatt aatagctatt atatagcttc agctcctttt ttcatacctt
//
LOCUS       DAZOZX010000372         1284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZOZX010000372 DAZOZX010000000
VERSION     DAZOZX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.380"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(318..>1284)
                     /locus_tag="ACHUPT_004480"
     CDS             complement(318..>1284)
                     /locus_tag="ACHUPT_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0821721.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          383 a          275 c          276 g          350 t
ORIGIN      
        1 tatttttgtg atgttttaga tgtgtaataa tgaaccaggt taaagtgtat ttaaaaatgt
       61 gttgtattag cgactgaaat ctgcctttta aaaaggagga gggtactgag acaaagagtc
      121 attgcttctc cgataaaatg tcagagaagc atgattacgt ggttattgtc tgttgatgac
      181 ttgttgaccg agccgtcatc attctcaatg cattagttaa atcatttgtg atttttaagg
      241 attatcctgt ctatgatttt ctacttagaa ggatatggca ctaactatgc atcttatagt
      301 ttactgctgg catctattta aaatagcaat tgagtcaaat agcccatcaa gatagccata
      361 cctaatataa ccgtcaggaa tgcagctatc atcggcattc tgaacattga tttaagtaat
      421 atcacttccg tcaggcttgc gccggcactg ccgatgatta atgccattaa tgctcccaga
      481 cccattcctt ttgtcatcag aacagatgcc agagggataa ccgcctcagc ccggatatac
      541 agcggaatac cgacaacggc gcttaatggg atggcgaagg gattatctgc tcctgcatga
      601 gcggcaatcc acgctgaggg aataaagcca taaataaaag atcctattaa tactcctaac
      661 aaaagatatg gcagtacatc tttaaactgt tgcaacgcat ctttggtggc tagttttatg
      721 ggattaatac ttactacagc tttggtactg caacaggtaa ctacggggtt ctcaatggct
      781 ttagccgctg gactacaaca actcactttt atgggcgtat atgtcgttcc cggcgaagtt
      841 tttgctggag cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac
      901 cccagagaat ccaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac
      961 aacaaggtga ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt
     1021 aatggtgaaa caaataaaaa agtgagagtc ggaccaaacc cggctttcgc tgacaacaat
     1081 ccacgtaaca tgggtattgt cgagcaacta cagaacgggg taacggctcc caacagagca
     1141 gccaggagat agcctctccc ttttctggcc cccatcatct gctggatttt atggtctggc
     1201 accttttgtc ttatcaggct gacaccggca ctaatcacta taaacagcaa cgaaagctcg
     1261 acggcgagaa acacaaacat ctct
//
LOCUS       DAZOZX010000373         1279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZOZX010000373 DAZOZX010000000
VERSION     DAZOZX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.381"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUPT_004481"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUPT_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR0821722.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          359 a          230 c          286 g          404 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactatctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAZOZX010000374         1251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZOZX010000374 DAZOZX010000000
VERSION     DAZOZX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.382"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..924
                     /gene="virB"
                     /locus_tag="ACHUPT_004482"
     CDS             <1..924
                     /gene="virB"
                     /locus_tag="ACHUPT_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR0821723.1"
                     /translation="DLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTFVN
                     QKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAGAD
                     LEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENLSR
                     AKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILKEE
                     IKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTNRT
                     LIFTLSKINKTVQREIDEAIRDIISRHLSSS"
BASE COUNT          480 a          225 c          211 g          335 t
ORIGIN      
        1 gatttgtgca acgacttgtt aagtataaag gaaggccaaa agaaagagtt tacactccat
       61 tctggtaata aagtttcctt tatcaaagcc aagattcctc ataaaaggat ccaagattta
      121 accttcgtca accaaaaaac gaatgtacgc gatcaagaat ccctaacaga agaatcatta
      181 gccgatatca taaaaactat aaagctacaa caattcttcc ctgtaatagg aagggagatt
      241 gatggtagaa ttgaaattct ggatggcact cgtagaagag catctgcaat atatgcagga
      301 gcagatcttg aagttctata ttcaaaagaa tatatatcta ctcttgatgc cagaaaacta
      361 gcaaacgata tacaaacagc aaaagagcat agcatccgag aacttggtat tggtcttaat
      421 tttctgaaag tatcagggat gtcctataaa gacatagcca aaaaagagaa tctgtctcgc
      481 gcgaaagtca ctcgtgcctt tcaggcagca agcgttccac aggaaataat atctctattt
      541 ccaatcgcgt cagaacttaa ctttaatgac tacaagatat tattcaatta ttataaagga
      601 cttgaaaagg ctaatgaatc tcttagttct acactaccaa tattaaagga agaaataaaa
      661 gaccttgata caaatttgcc cccggacata tataaaaaag aaattttaaa catcataaag
      721 aaaagcaaaa acagaaagca aaacccttcg ctaaaagttg actcgttatt tatttctaaa
      781 gacaaacgga cttacataaa aagaaaagag aataaaacaa acagaactct aatatttaca
      841 ttatctaaaa taaacaaaac agttcagaga gaaatagatg aagctattcg ggatataatt
      901 tctcgccatc tatcgtcttc ataaacatcg aacactgatg taaactgccc aattcagata
      961 ataaaaatct tcgttgaact tgactttcag ccggtggggt aattccgtcg gctatctgtt
     1021 attcaatccc cacgcactgt gtttccttaa aattacggtg aaaactcata ccctgctgac
     1081 cacgagaaaa ttatctggtg aaattagtaa tattcatatc attgaaaatg aattgtgcaa
     1141 ctaagggatg cggcatgatg gtcaaaattt tatgtgaata tgagtgactg gccgaaaata
     1201 ttcagcgtat ttttaatcgt ttcaataaaa acagcgagga ttagcagtca a
//
LOCUS       DAZOZX010000375         1248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZOZX010000375 DAZOZX010000000
VERSION     DAZOZX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.383"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            114..>1248
                     /gene="pdeF"
                     /locus_tag="ACHUPT_004483"
     CDS             114..>1248
                     /gene="pdeF"
                     /locus_tag="ACHUPT_004483"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0821724.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          280 a          279 c          281 g          408 t
ORIGIN      
        1 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
       61 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
      121 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
      181 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
      241 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
      301 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
      361 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
      421 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
      481 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
      541 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
      601 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
      661 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
      721 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
      781 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
      841 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
      901 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
      961 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
     1021 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
     1081 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
     1141 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
     1201 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaat
//
LOCUS       DAZOZX010000376         1229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZOZX010000376 DAZOZX010000000
VERSION     DAZOZX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.384"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..308
                     /locus_tag="ACHUPT_004484"
                     /pseudo
     CDS             1..308
                     /locus_tag="ACHUPT_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            341..742
                     /locus_tag="ACHUPT_004485"
                     /pseudo
     CDS             341..742
                     /locus_tag="ACHUPT_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          356 a          262 c          306 g          305 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatag
      301 cgttctaagc cttcccacca tgatcgggga agccccagat atgaacaggc acattcagag
      361 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      421 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      481 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      541 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gccccccccc ggtttaatct
      601 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      661 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      721 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      781 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      841 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      901 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      961 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
     1021 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
     1081 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
     1141 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
     1201 attctgcaga gtattttgat atgataaat
//
LOCUS       DAZOZX010000377         1225 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZOZX010000377 DAZOZX010000000
VERSION     DAZOZX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.385"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..482)
                     /gene="ospZ"
                     /locus_tag="ACHUPT_004486"
     CDS             complement(<1..482)
                     /gene="ospZ"
                     /locus_tag="ACHUPT_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HFR0821725.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRAEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFSKENSDFLYIIVVFRNDSSQGELR
                     A"
     gene            538..690
                     /locus_tag="ACHUPT_004487"
                     /pseudo
     CDS             538..690
                     /locus_tag="ACHUPT_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          376 a          239 c          219 g          391 t
ORIGIN      
        1 gcccgtagtt ctccttggga agaatcattt ctgaatacaa caattatata caaaaaatca
       61 ctattttctt tagagaagat cttacaacta atctctggga gctcacataa tgcatgtaag
      121 tcaggatggt acaaactatc cacagaaaat tgccccatag gacgttttcc agatataact
      181 gtcaaccagc ttccttccgg tgtactggct tcggctctgg aaataggctg aaaatcttcc
      241 ggacatggag aatacattgt cttcatataa ttaagtcgtc tggatttctc attaataaac
      301 tcctctattt ttaattgtgc catacttctg gttataccag agatatactt cttgtcatat
      361 atgtttggtg attcattaaa gtaaccatgt tcaactctag gataaatatt tctcactata
      421 tattcagtat ttgcagtatt tattggaaat acgttcttaa tattcttgat gggactaatc
      481 attttaatct ctatactcaa atataaacat taccatgaac aatataattg ttcctaagtg
      541 gaatgtctcc acggagaaca ctttatctat cgggaaatag tgcgggcaac agtgtttaat
      601 tatatcgagt gcaattacaa tcgctggcga cggcaccgtt ggtgtggcgg cctcagtccg
      661 gaacaatttg aaaaccagaa cctcgcttag gcctgtgtcc atattacgtg ggtaggctca
      721 cagagcctca cttacgactg cgtccatatt atgtgggtag gatcaataaa gacgttttat
      781 atgtgtctgc ttgcagccag aaaatttata ccctgcaggt atcataaaca tgcttgataa
      841 cttaattggg gctccctctt ccttttatgg tgtaattgtt tgattgaata cttccgggga
      901 tttcagtatg aaatgaagta tatttaatat actttacaaa aagtttgtat tagtggaata
      961 aggacaatag cttatccaaa ctgccaagcc tctttattat aagtaagatc tggcactgga
     1021 atattctcaa aaggactaac atcatcacca cgaccttcaa ccaaccattt tattgcattt
     1081 aaatcccaga atagttcagt agtggtcaat ttgggaaacg cacaatattg tacggttacg
     1141 ttatcgcaat agagtgaact ctcgccctat acggcgtttt caaggattag tccttaatcg
     1201 gacaaccaca ttaaagcata ataaa
//
LOCUS       DAZOZX010000378         1218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZOZX010000378 DAZOZX010000000
VERSION     DAZOZX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.386"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(279..353)
                     /locus_tag="ACHUPT_004488"
     tRNA            complement(279..353)
                     /locus_tag="ACHUPT_004488"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(318..320),aa:Gly,seq:tcc)
     gene            complement(357..431)
                     /locus_tag="ACHUPT_004489"
     tRNA            complement(357..431)
                     /locus_tag="ACHUPT_004489"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Thr,seq:tgt)
     gene            complement(434..510)
                     /locus_tag="ACHUPT_004490"
     tRNA            complement(434..510)
                     /locus_tag="ACHUPT_004490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(474..476),aa:Arg,seq:tct)
     gene            complement(512..588)
                     /locus_tag="ACHUPT_004491"
     tRNA            complement(512..588)
                     /locus_tag="ACHUPT_004491"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(552..554),aa:Ile,seq:cat)
     gene            complement(778..>1218)
                     /locus_tag="ACHUPT_004492"
     CDS             complement(778..>1218)
                     /locus_tag="ACHUPT_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0821726.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          325 a          323 c          256 g          314 t
ORIGIN      
        1 aaccgccagc caaggcagtt aagagtgtgt tcccggggtt tgcttagaat attttgtaag
       61 ttgtccgaag agatatttac aacaccagaa tgatgcatta tcagtcccct gccagaggtt
      121 cactacgtaa cctgaccgac aatgcaccat tctggtgctg aaaaaagagc actggaactg
      181 caacaaaaca acatcacaga acaggagatg gtaaggagtc agacattccc acacaatatt
      241 gtgtcagtgc aaataacaac cttcgtctca aatctcgctg gagcgggcag cgggaatcga
      301 acccgcatca tcagcttgga aggctgaggt aatagccatt atacgatgcc cgcatatggt
      361 gcgactaccg gaatcgaact ggtgacctac tgattacaag tcagttgctc tgcctgctga
      421 gctaagtcgg cgctggccca ccaccgagga ctcgaacctc gcaccgtcaa cttagaaggt
      481 tgatgctcta tccggatgag ctagtggtgg ttggtggccc ttgctggact tgaaccagcg
      541 acctggcgat tatgagtcgc tcgctctgac caactgagct aaagggccgg aggcagaata
      601 ataaccatat gtcatcacat ctgcaaactc atctgatcac cagcacgttt aacgtcctgt
      661 gccgtttttc aggtataaaa aaaaccgcat aaagcgggct ctttcaaatg tccatgtctg
      721 ctattcgcct cgcggtacag ctttgcgaag cgtacaagaa ttgaagcagt ttttacgtca
      781 aaaagcaata acttttttct ctataccaaa agccataacc attggtttgt acaaaataaa
      841 ttctgccacc tttagccaat gctcaatgcg tctttcacag gttcttaaac tccatttcgg
      901 gtgtgcatca ttcagcagtt cagccatttt gcatttggtc atcccccgcc cctcataccg
      961 ttgccgaagg acgctaatca atcctggatg ctctgccagc acctcactta tgacccgatc
     1021 aatacataac gcctctgcat cagtacaatg cgccagccag ctcttttgct taccgttaat
     1081 catatcccgc aaaaaagcct caagttcaga cttgttcaga cctgcttttt tcatcctccg
     1141 gagcgcctcg ttaattgccg tttttgtcag ctttttagag gtcaacatca ggttaaacat
     1201 attccccgtc ttaccgcc
//
LOCUS       DAZOZX010000379         1217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZOZX010000379 DAZOZX010000000
VERSION     DAZOZX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.387"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(108..479)
                     /locus_tag="ACHUPT_004493"
     CDS             complement(108..479)
                     /locus_tag="ACHUPT_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821727.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(540..1037)
                     /locus_tag="ACHUPT_004494"
     CDS             complement(540..1037)
                     /locus_tag="ACHUPT_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821728.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1066..1217)
                     /locus_tag="ACHUPT_004495"
                     /pseudo
     CDS             complement(1066..1217)
                     /locus_tag="ACHUPT_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase"
BASE COUNT          286 a          316 c          290 g          325 t
ORIGIN      
        1 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
       61 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
      121 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
      181 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
      241 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
      301 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
      361 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
      421 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
      481 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
      541 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
      601 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
      661 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
      721 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
      781 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
      841 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
      901 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
      961 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
     1021 ttcaacagat cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg
     1081 aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     1141 cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     1201 tgtccaagat aagcctg
//
LOCUS       DAZOZX010000380         1202 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZOZX010000380 DAZOZX010000000
VERSION     DAZOZX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.388"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..1202
                     /gene="sat"
                     /locus_tag="ACHUPT_004496"
                     /pseudo
     CDS             1..1202
                     /gene="sat"
                     /locus_tag="ACHUPT_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          465 a          187 c          237 g          313 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga agtgttctgt
      361 ttaaggataa tttggatttt ggttgcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagagattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa agaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 ca
//
LOCUS       DAZOZX010000381         1171 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZOZX010000381 DAZOZX010000000
VERSION     DAZOZX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.389"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..1103)
                     /locus_tag="ACHUPT_004497"
     CDS             complement(<1..1103)
                     /locus_tag="ACHUPT_004497"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0821729.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT          284 a          307 c          303 g          277 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagtgcat cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt attttcgtcg caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgcac cggaccgcac accaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt gttaatggat aaatccatca acttgtggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctgaaatgc gacatcgtcg cgcatttcgt gcagcggaaa acgttttgat
     1021 tctgcgatag tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa
     1081 tccgttactt gcttcttatc cattttaaac tccttaaaat gattggatcg cattaaaaaa
     1141 gtaggattta tcgataaagt aagcaagtgc a
//
LOCUS       DAZOZX010000382         1166 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZOZX010000382 DAZOZX010000000
VERSION     DAZOZX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.390"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..758)
                     /locus_tag="ACHUPT_004498"
     CDS             complement(<1..758)
                     /locus_tag="ACHUPT_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR0821730.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKR"
     gene            1092..>1166
                     /gene="ytiD"
                     /locus_tag="ACHUPT_004499"
     CDS             1092..>1166
                     /gene="ytiD"
                     /locus_tag="ACHUPT_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFR0821731.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLR"
BASE COUNT          355 a          196 c          219 g          396 t
ORIGIN      
        1 atgcgtttga gtggcgcatc ttcatcaccg gggataacgt tatatcccat ttttgaatgg
       61 atctccgcgg taccaggata ggcttctata ccaatgattg ggtcatttcc catctcaata
      121 ttagcattaa gtaatgcggc aagggcgaat ccggaagaat ttgctacccc tgagacagtt
      181 attatctgat caatatagat atcaattctt ggctgctcat tggtccatct ttttgcctga
      241 gaagctggaa gggttgaaat atatcctatc gtggttccgg cataaccagg aacattgcaa
      301 ccagggccta atgttatttc atgaactgta tagtcctcaa caggatcgaa agggctcttc
      361 ataaataaat ttagccgtaa accttctgct aactctgtag cagcatgcgc ctttttacat
      421 ataccaccat atatttcgtc taaagctgag gtgattttca agtttcgata gagcatacgt
      481 cctgttgttg taataccatt gaattctctg catgtatctg tcacataatt aaatggccag
      541 gactgtttgc caatattaag ttttctaatt tcgacaacgc tttttccatc gtaattatgg
      601 ttattgccac gagcaagagg cagaagatag atattttccg tatccaggcg tgcatattca
      661 tatcgacgtg ttaactcact gtcgactttt ggatgatgaa ggaagtgatt atttgtattg
      721 aattggttat tagcggcatt gcttaatggc atagacataa tagttacctt ttatgagtat
      781 ttaactgatg tttagaaaaa tagataaatt tttctgtagt aaaaagagaa gtaaacaaat
      841 gacatgcatg tttctgttgt agtgatatca actctacaca gtgatattaa agggtaggaa
      901 acactctaaa gtatcaaaaa acgctcattt aaaattattt gcatgcaatt taaaagcata
      961 tcttattact aattggaatt tgatattgct atattgaggt ctatattaat aatgcctgtg
     1021 aatggtattt ttgatgtatt tgatatgtta tcaatttata ttatttacaa actaattgtt
     1081 tcaaataata catagctgat tatgcggaaa taaataattt cccaccggaa ttaagtagca
     1141 gcggtgacaa gtattttcat ctacgt
//
LOCUS       DAZOZX010000383         1164 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZOZX010000383 DAZOZX010000000
VERSION     DAZOZX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.391"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..238
                     /locus_tag="ACHUPT_004500"
     CDS             <1..238
                     /locus_tag="ACHUPT_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR0821732.1"
                     /translation="IPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKI
                     NSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            459..800
                     /gene="symE"
                     /locus_tag="ACHUPT_004501"
     CDS             459..800
                     /gene="symE"
                     /locus_tag="ACHUPT_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR0821733.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(847..>1164)
                     /locus_tag="ACHUPT_004502"
     CDS             complement(847..>1164)
                     /locus_tag="ACHUPT_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR0821734.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT          341 a          287 c          223 g          313 t
ORIGIN      
        1 cattccattt cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc caaaacttaa
       61 ttctgacagt ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat
      121 atttgataag ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat
      181 ttatcataaa acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt
      241 ccctcgccct cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa
      301 tttatatttc acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt
      361 atttactgat gatgtatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca
      421 ccgctaatca caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg
      481 cacaaccgtt agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg
      541 cgagtcgtta cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg
      601 aagccgccgg ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg
      661 tcctcaccgc ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg
      721 tgtgtaagct gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag
      781 gtaaacagaa agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc
      841 gccgcgttat accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat
      901 aatgtaagga agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg
      961 cccaacattt gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat
     1021 attaagcgac aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc
     1081 tttaaacgca atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac
     1141 ttcatcgcga agatgatcga ataa
//
LOCUS       DAZOZX010000384         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZOZX010000384 DAZOZX010000000
VERSION     DAZOZX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.392"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            116..>1160
                     /gene="cadB"
                     /locus_tag="ACHUPT_004503"
     CDS             116..>1160
                     /gene="cadB"
                     /locus_tag="ACHUPT_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0821735.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          256 c          298 g          360 t
ORIGIN      
        1 ctttaggatt aatccttttt tcgtgagtaa tcttatcgcc agtttggtct ggtcaggaaa
       61 tagttataca tcatgacccg gactccaaat tcaaaaatga aattaggaga agagcatgag
      121 ttctgccaag aagatcgggc tatttgcctg taccggtgtt gttgccggta atatgatggg
      181 gagcggtatt gcattattac ctgcgaacct agcaagtatc ggtggtattg ctatctgggg
      241 ttggattatc tctattattg gtgcaatgtc gctggcgtat gtatatgccc gactggcaac
      301 aaaaaacccg caacaaggtg gcccaattgc ttatgccgga gaaatttccc ctgcatttgg
      361 ttttcagaca ggtgttcttt attaccatgc taactggatt ggtaacctgg cgattggtat
      421 taccgctgta tcttatcttt ccaccttctt cccagtatta aatgatcctg ttccggcggg
      481 tatcgcctgt attgctatcg tctgggtatt tacctttgta aatatgctcg gcggtacctg
      541 ggtaagccgt ttaaccacta ttggtctggt gctggttctt attcctgtgg tgatgactgc
      601 tattgttggc tggcattggt ttgatgcggc aacttatgca gctaactgga atactgcgga
      661 taccactgat ggtcatgcga tcattaaaag tattctgctc tgcctgtggg ccttcgtggg
      721 tgttgaatcc gcagcagtaa gtactggtat ggttaaaaac ccgaaacgta ccgttccgct
      781 ggcaaccatg ctgggtactg gtttagcagg tattgtttat atcgctgcga ctcaggtgct
      841 ttccggtatg tatccgtctt ctgtaatggc ggcttccggt gctccgtttg caatcagtgc
      901 ttcaactatc ctcggtaact gggctgcgcc gctggtttct gcattcaccg cctttgcgtg
      961 cctgacttct ctgggctcct ggatgatgtt ggtaggccag gcaggtgtac gtgccgctaa
     1021 cgacggtaac ttcccgaaag tttatggtga agtcgacagc aacggtattc cgaaaaaagg
     1081 tctgctgctg gctgcagtga aaatgactgc cctgatgatc ctcatcactc tgatgaactc
     1141 tgccggtggt aaagcatctg
//
LOCUS       DAZOZX010000385         1159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZOZX010000385 DAZOZX010000000
VERSION     DAZOZX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.393"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..747
                     /gene="phoC"
                     /locus_tag="ACHUPT_004504"
     CDS             <1..747
                     /gene="phoC"
                     /locus_tag="ACHUPT_004504"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR0821736.1"
                     /translation="KRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAIDS
                     LALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFGS
                     PITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSRN
                     GSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIVG
                     SAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(802..906)
                     /locus_tag="ACHUPT_004505"
                     /pseudo
     CDS             complement(802..906)
                     /locus_tag="ACHUPT_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(917..>1159)
                     /locus_tag="ACHUPT_004506"
     CDS             complement(917..>1159)
                     /locus_tag="ACHUPT_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0821737.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          307 a          264 c          253 g          335 t
ORIGIN      
        1 aaaagacagc tttttactct tagtattgtc ggggtatttt ctttaaatac ctttgcatca
       61 ttccctccgg gaaatgatgt gacaacaaaa cctgaccttt actacctgac aaacgataat
      121 gctattgaca gtctggcatt attaccgcct ccaccacaaa tcggaagtat tgccttcctg
      181 aatgaccagg ccatgtatga aaaggggcgt ttattgcgaa atactgaaag agggaagctg
      241 gcagctgagg atgccaatct cagtagtggt ggcgtggcaa atgtattttc tgctgctttt
      301 ggttctccga taacggctaa agattcacca gaattgcata aattactgac aaatatgatt
      361 gaggatgccg gagacctggc aacgcgttca gcaaaagaat attatatgcg tattcgacct
      421 tttgcttttt atggtgtttc tacgtgtaat acaaaagaac aggatacatt atccagaaat
      481 ggctcttatc catcaggtca tacatcgatt ggttgggcaa cagcacttgt attgtccgaa
      541 ataaatccgg cacgccagga taccattctg aaacggggtt atgaactggg ggacagcagg
      601 gttatttgcg gttatcactg gcaaagtgat gttgacgcag cacgtattgt tggctctgct
      661 attgtagcaa cactgcactc aaatcctgtg ttccaggcac aattacaaaa agcgaaagat
      721 gaattcgcta acaatcagaa aaaataatgc ttttagccag tacgccttat aaccccgacc
      781 ttcagggcta atgccagtca gttaagcaac tgactggctc tttttcaggg ctgtggggta
      841 tttccagtgc ctctccttta ccactctcgg aaaggccctt tcccttcttg tcggtaattt
      901 cactaggctg tgtcccttaa ttacgtaagc gttgataaaa gagtcggatg cagcccagtt
      961 tcactatcgc caggtaattt ctcgccgttt tgtcgtaacg cgtggcgatg cggcgatatt
     1021 ctttcagccg cccaaagcac cgttcaacga cattacgatt gcagtacgca tcacgatcaa
     1081 gctgtgcacg tccatccgat gccattttct catttgactt tcgggggatt actgccttta
     1141 taccgttatt tttcagctc
//
LOCUS       DAZOZX010000386         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZOZX010000386 DAZOZX010000000
VERSION     DAZOZX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.394"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..730
                     /locus_tag="ACHUPT_004507"
     CDS             <1..730
                     /locus_tag="ACHUPT_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR0821738.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHUPT_004508"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHUPT_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZOZX010000387         1122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZOZX010000387 DAZOZX010000000
VERSION     DAZOZX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.395"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..72
                     /locus_tag="ACHUPT_004509"
     CDS             <1..72
                     /locus_tag="ACHUPT_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mxiK"
                     /protein_id="HFR0821739.1"
                     /translation="LSRLNEGMQYAKRHFTGIQTSCL"
     gene            83..709
                     /gene="mxiN"
                     /locus_tag="ACHUPT_004510"
     CDS             83..709
                     /gene="mxiN"
                     /locus_tag="ACHUPT_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR0821740.1"
                     /translation="MKRIEKELCKTIKDRDTESKKKAICVIKEATKKAESLRIDAVCD
                     GYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYITSLLSENLHDERIISTLLEQWLS
                     SLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDVKIILKYSEGNNYIFCSGNQVVEF
                     SPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQIAEDLLKENPVND"
     gene            702..1109
                     /gene="mxiM"
                     /locus_tag="ACHUPT_004511"
     CDS             702..1109
                     /gene="mxiM"
                     /locus_tag="ACHUPT_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFR0821741.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
BASE COUNT          428 a          139 c          214 g          341 t
ORIGIN      
        1 ttgtcccgat taaatgaagg tatgcaatat gcaaaaaggc actttaccgg tatccagaca
       61 tcatgcctat gatggtgttg ttataaagag aattgaaaag gaattatgta aaaccataaa
      121 agatagagac actgagagta agaagaaagc aatttgtgtt ataaaggagg ctacgaaaaa
      181 agctgaatca ctgcgtatag atgctgtatg tgatggttat caaattggta tacagactgc
      241 ttttgagcat attattgact atatttgtga atggaagtta aagcaaaatg aaaatagaag
      301 aaatattgag gactatataa ctagtttgct ttcagaaaat ttacatgatg agaggatcat
      361 tagcacttta ttggagcagt ggcttagttc gttgcgtaat acagtaacag aattaaaagt
      421 ggttttgccc aagtgtaatt tagcactgag gaaaaagtta gaacttgatc tccataaata
      481 tcgctcagat gtcaagatta ttttaaaata ttctgaaggg aataattata tattctgtag
      541 tggtaatcaa gtagtggagt ttagtcctca ggatgtaata tcaggtgtta aaatagaact
      601 tgcggaaaaa ttaacaaaaa atgataaaaa atatttcaaa gagttagcac ataagaaact
      661 gagacaaatt gcagaagact tgttaaaaga gaatcctgtt aatgattaat caaataaatg
      721 caagcaatgc tctgcagcaa agattaaata gtgaagaagt tgttaattta aatgagagat
      781 tatcatcttc gcaatcattt gatgaggata taatctatga aataatgcaa tatttctcac
      841 agagtgaatt aaattccata gataatgatg aactgcacaa taagattgaa caattattca
      901 attcacgttt tccttatctt acagctgcac aaaaaagttc acttcttaat aaactcatag
      961 atgcaaatca atatgtcgat ttgcatgagg gattttatgc atcattgtcc atatataaca
     1021 atattgattt ctatattaag actacaacct tcgattcatt aattagtgtc tttgaagcag
     1081 ggagagaggc agatgattcg acatggtagt aataagttga aa
//
LOCUS       DAZOZX010000388         1109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZOZX010000388 DAZOZX010000000
VERSION     DAZOZX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.396"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..1019
                     /locus_tag="ACHUPT_004512"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACHUPT_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="ACHUPT_004513"
     CDS             992..>1109
                     /locus_tag="ACHUPT_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821742.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZOZX010000389         1105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZOZX010000389 DAZOZX010000000
VERSION     DAZOZX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.397"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(201..323)
                     /locus_tag="ACHUPT_004514"
     CDS             complement(201..323)
                     /locus_tag="ACHUPT_004514"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821743.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(396..>1105)
                     /gene="phoN2"
                     /locus_tag="ACHUPT_004515"
     CDS             complement(396..>1105)
                     /gene="phoN2"
                     /locus_tag="ACHUPT_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR0821744.1"
                     /translation="ATNMIFIPSANALKAEGFLTQQTSPDSLSILPPPPAENSVVFQA
                     DKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTPILYQLLTQVLQ
                     DSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHASFGWAVALILA
                     EINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLHNTPEFTKSLSE
                     AKKEFEELNTPTNELTP"
BASE COUNT          292 a          241 c          247 g          325 t
ORIGIN      
        1 attaaaaaat ggagcggtat caggcaaacg atttgagagg tgacctaaaa tatatttagg
       61 ttgtgccctg aatctgaagt tgtcatttct gttatattga tcgacatagg ggatggtgca
      121 gaccgttccc ctgcaacacc acattgcatg tggtgaacgg aaccgccccg agtttcctag
      181 agaatgtttt atctgtgaac tcaggttgcc agatcatcgt ttccgatggc agcataataa
      241 gctttttctg cttctgccgg agggatatgt cccagccttt ccagtaatcc cttaaattca
      301 ggggtaaaac gtggtgtgcc catagactcc tcctatgctc atatagggta gatttgtcta
      361 tgggccctcc atagcctgat acaggctgtc cagctttatg gggtcagttc attggtagga
      421 gtatttaatt cttcaaactc ttttttggct tcgctaaggc ttttggtaaa ttcaggtgta
      481 ttatgaagta ctgcaacaac cgatgctccc attaaacgcc cagcctctac atcgctttgc
      541 caatgcgcac cgcagatgac ccgactttct ccaaactcat atccacgtcg aagtatttcc
      601 gctttacgtt gaggattaat ctccgcaagt atcagtgcta ctgcccaacc aaaggatgca
      661 tgaccagagg gataagagcc agtgatagcc attttctcat ctttatcagg tgtacaggtt
      721 gcgtctttat aaataacgaa tggacgaact cttttataat attctttggc gttacgcacg
      781 gcgtaatcat ggctatcctg tagtacttgt gttaacaact gatataagat tggggtattt
      841 tcccttgaaa tatccatgcc ataagcatct gaaaatgcaa gaccaaaatt ctcgtagtat
      901 gcatcttcgc tagcgagacg tacacgatta gcatcccgga gcgagcggcc gaattcataa
      961 tgagctttgt cagcctgaaa tactactgaa ttctctgccg gaggcggcgg aagtattgac
     1021 aaactgtctg gtgaagtttg ttgagtgaga aaaccttctg ccttcagagc atttgctgag
     1081 gggataaaaa tcatatttgt agcaa
//
LOCUS       DAZOZX010000390         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZOZX010000390 DAZOZX010000000
VERSION     DAZOZX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.398"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHUPT_004516"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHUPT_004516"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0821745.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACHUPT_004517"
     tRNA            complement(986..1062)
                     /locus_tag="ACHUPT_004517"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAZOZX010000391         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZOZX010000391 DAZOZX010000000
VERSION     DAZOZX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.399"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1038
                     /locus_tag="ACHUPT_004518"
     CDS             <1..1038
                     /locus_tag="ACHUPT_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR0821746.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
BASE COUNT          297 a          264 c          288 g          236 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgc
//
LOCUS       DAZOZX010000392         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZOZX010000392 DAZOZX010000000
VERSION     DAZOZX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.400"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHUPT_004519"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHUPT_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR0821747.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          301 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
//
LOCUS       DAZOZX010000393         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZOZX010000393 DAZOZX010000000
VERSION     DAZOZX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.401"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(226..651)
                     /locus_tag="ACHUPT_004520"
     CDS             complement(226..651)
                     /locus_tag="ACHUPT_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR0821748.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(648..1031)
                     /locus_tag="ACHUPT_004521"
     CDS             complement(648..1031)
                     /locus_tag="ACHUPT_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR0821749.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          307 c          265 g          243 t
ORIGIN      
        1 tcgcgtatgg ttgattgaca ccacagtgcg aattcggcga atgcgagatc acggtacgat
       61 tccgcaccac actaccaatc aataccccgg gttttattca ggtaagcagg actgcggata
      121 tccggtgtcg cgcctttgtg tcacgaacgg ggtgggtgcg aaacaccaga taaaatacag
      181 gttggcaata cctctgaacg ccctgagcag gtcgggcatt tcggattaag tactcgcacc
      241 tccgcagtcc tcaaacaagt ctggctggta gctgtaaaca gacttcgtac ataccgctct
      301 gcaataaatc cccgtaccac aggcctcaca gaacttcttc ccatgaaaaa actgctcaca
      361 catcacacaa tgccactcca gcacgaccgg taatggcgat aaaaacatcg ccatatcctc
      421 aatgtaaggg tgggacttct ccggattcag caccacacag tccgccttct gttgctcact
      481 cagggcatgt aaatcatgct caaaccacgc cccctgagca tctgtctgca gaatcaaccg
      541 gccatggcag gaaaggcaga aacactgccg gatatttctg ctgagcgcag agtcggcact
      601 gataatgtgc tcacccggcg tgatccccag ccccgttttt ataccgttca ttctgctccc
      661 ccctcctcac tgaagcgccc ggtctggcag tgtgtgcagt actgctcccc ataataatcg
      721 tggtgacatt gcctgcagtg ccagctggct ttacgtacta cgggtaaggt atccgggacg
      781 aattgctgca gacgtttaat caaccgtatt tctctgcgtt ccggcctgac ataagggcac
      841 tcatgaccgt gctccgtcag cccttcgtca gcgtgttcaa accagggccg ttcagtgtcg
      901 tattgcgggt ggtatctgag tgcactgccg caaaggtggc aggtgtagct atcgtaaggt
      961 gcagtctgtg cggtacgggc accggtcaga cgtccgttgc catcaagtgc gagaaaagat
     1021 tttgcgtaca tagtatatgt tcctcagccg ccagacgaca cgcaggca
//
LOCUS       DAZOZX010000394         1066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZOZX010000394 DAZOZX010000000
VERSION     DAZOZX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.402"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(570..749)
                     /locus_tag="ACHUPT_004522"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACHUPT_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACHUPT_004523"
     CDS             843..>1066
                     /locus_tag="ACHUPT_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0821750.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAZOZX010000395         1036 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZOZX010000395 DAZOZX010000000
VERSION     DAZOZX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.403"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..219
                     /gene="mxiH"
                     /locus_tag="ACHUPT_004524"
     CDS             <1..219
                     /gene="mxiH"
                     /locus_tag="ACHUPT_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR0821751.1"
                     /translation="LSSLSETFDDGTQTLQGELTLALDKLAKNPSNPQLLAEYQSKLS
                     EYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            232..525
                     /gene="mxiI"
                     /locus_tag="ACHUPT_004525"
     CDS             232..525
                     /gene="mxiI"
                     /locus_tag="ACHUPT_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR0821752.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            531..>1036
                     /gene="mxiJ"
                     /locus_tag="ACHUPT_004526"
     CDS             531..>1036
                     /gene="mxiJ"
                     /locus_tag="ACHUPT_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR0821753.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKR"
BASE COUNT          355 a          159 c          205 g          317 t
ORIGIN      
        1 ttgagttcat tatctgaaac ttttgatgat ggaactcaaa cattacaagg tgaactaaca
       61 ttggcactag ataaattagc taaaaatcct tcgaatccac agttgctggc tgaataccaa
      121 agtaaattat ctgaatatac attatatagg aacgcgcaat ccaatacagt gaaagtgatt
      181 aaggatgttg atgctgcaat tattcaaaac ttcagataat agggagcatt catgaattac
      241 atttatccag tcaatcaggt tgatattatc aaagccagtg attttcaatc tcaagagata
      301 tcaagtctgg aagacgtcgt gtcggctaaa tatagtgata ttaagatgga tacagatatt
      361 caagtatcac aaataatgga gatggtaagc aatccagaat cattaaaccc agaatctttg
      421 gccaagttac agacgacgct ctcaaattat tcaataggag tatcattagc tggcacgtta
      481 gcaagaaaaa cagtttcggc tgttgaaact ttattaaagt cttaatttat atgattaggt
      541 ataaaggttt tattttattc ttgttgctga tgttgattgg atgtgagcaa cgtgaagagt
      601 taatttctaa tttatctcaa agacaggcaa atgaaataat atctgtgcta gaacgccata
      661 atattactgc tagaaaagtt gatggaggta aacaggggat ctcggtacaa gtcgaaaagg
      721 ggacatttgc atcggcagtt gatttgatgc gcatgtacga tttgccaaat ccggagagag
      781 ttgatatctc acaaatgttt cctacagatt cattagtgtc ttctccaaga gctgaaaagg
      841 cccgtttata tagtgctatt gagcaacggc tggaacagtc tttagtttct attggtggtg
      901 ttatttcggc aaaaatacat gttagctatg atcttgaaga aaaaaatata tcttcaaaac
      961 cgatgcatat atcagtaatc gctatatatg actcaccgaa agagtctgaa ctattagtta
     1021 gtaatattaa gcgatt
//
LOCUS       DAZOZX010000396         1029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZOZX010000396 DAZOZX010000000
VERSION     DAZOZX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.404"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            207..>1029
                     /locus_tag="ACHUPT_004527"
     CDS             207..>1029
                     /locus_tag="ACHUPT_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0821754.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT          345 a          247 c          149 g          288 t
ORIGIN      
        1 tttttttaca ttaaaccgat ccagtttagg atagtgtaaa gcagaagttc ttcaatatat
       61 aacatatgga gttaatatgc ttacacaaac tatatatccg cctcctccag aagttcctgt
      121 ctactgctga agggaaccaa taacattgca aagcagataa aaaagaatcc aacacctctc
      181 aacataaaga atacaccctg atatttatgc tcccgacaaa taacaatcac agattaattt
      241 caaattcgtt ctccacttat tcaatcgaca ctagccgcgc atatgaaaat tatctaaccc
      301 attggactga atggaaaaat aaccgcatac aagaagaaca acgagacatc gcttttcagc
      361 gactagtatc atgtctacaa aaccaagaga cgaacctgga cttgtctgaa ttaggcctga
      421 caacattacc tgaaatcccc ccgggaatta aatcaattaa tataagtaaa aataatttaa
      481 gcttaatctc cccattgcct gcgtccctta cacaacttaa tgtcagctat aacagactta
      541 ttgaactgcc tgctttgcct caaggactta aattattgaa tgcgtcccac aatcaactaa
      601 tcacactacc cacactcccc atatctttga aggagcttca tgtctcaaat aatcaattat
      661 gttctcttcc tgttttacca gaactactgg aaacattaga tgtatcatgt aatgggctgg
      721 cagttttacc acctttacca ttttctttac aagagattag cgcaataggg aatcttctta
      781 gtgaactccc ccctctacct cacaacattc actccatatg ggcaatcgac aatatgttaa
      841 ccgatattcc atacctgccg gaaaatttaa ggaacggtta ttttgacata aatcagataa
      901 gtcatatccc ggaaagcatt cttaatctga ggaatgaatg ttcaatagat attagtgata
      961 acccattgtc atcccatgct ctgcaatccc tgcaaagatt aacatcttcg ccggactacc
     1021 acggcccgc
//
LOCUS       DAZOZX010000397         1007 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZOZX010000397 DAZOZX010000000
VERSION     DAZOZX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.405"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHUPT_004528"
     CDS             complement(<1..104)
                     /locus_tag="ACHUPT_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR0821755.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHUPT_004529"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHUPT_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0821756.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT          276 a          241 c          180 g          310 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccg
//
LOCUS       DAZOZX010000398          990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZOZX010000398 DAZOZX010000000
VERSION     DAZOZX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.406"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            82..>990
                     /locus_tag="ACHUPT_004530"
     CDS             82..>990
                     /locus_tag="ACHUPT_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR0821757.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAZOZX010000399          980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZOZX010000399 DAZOZX010000000
VERSION     DAZOZX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.407"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(2..133)
                     /locus_tag="ACHUPT_004531"
     CDS             complement(2..133)
                     /locus_tag="ACHUPT_004531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821758.1"
                     /translation="MARSAKSRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFD"
     gene            215..408
                     /locus_tag="ACHUPT_004532"
                     /pseudo
     CDS             215..408
                     /locus_tag="ACHUPT_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            551..772
                     /locus_tag="ACHUPT_004533"
                     /pseudo
     CDS             551..772
                     /locus_tag="ACHUPT_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(793..855)
                     /locus_tag="ACHUPT_004534"
                     /pseudo
     CDS             complement(793..855)
                     /locus_tag="ACHUPT_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          292 a          233 c          227 g          228 t
ORIGIN      
        1 ttcaatcaaa gcgcagaact tctgcgtctt cttcgtcaaa aaaatcatct tcgtgaagct
       61 tcacgacata gcggggaagt ttgcttcttt gagaggcggg tttacgttta cgggatttag
      121 ctgaacgggc catataacca cctgaaagac aatgacattg cctgttttta taacggtaat
      181 tgcagaccat gacaaaccgc agccgtcagg ctgcctactc gagtcacaag gctctttgtg
      241 tccgatatgc cggcagcgca ttacccatga aactggctgg aacgtacacc ataaggtgaa
      301 gaaagttatg ggggcggtga agaattatct aaccttgtct tgttacatcc aaattgccat
      361 cgacagttac acagttgtga agccggctct ctcaaaaagg gcttataaag gcttgagcgg
      421 tgtaccggga aaccggtatg caccgttctt aggggagggc agtccagcaa tgaactgtcc
      481 ttacccgaca aacatccaaa acgaacggaa cgtgctggaa tccgcatata acccgttgta
      541 aagtgaggaa atggacacct cacttgctca tgagaacgcc cgcctgcggg cactgttgca
      601 gacgcaacag gacactatcc accagatggc tgaatacaac cgcctgctct cacagcggat
      661 ggcggcttat gcttccgaaa tcaaccgact gaaggcgctg gttgcgaaac tgcagcgtat
      721 gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga ttccattttc
      781 acgggcgatc tacgccacac aggctctggg ttgcagtgac tgctccatta aggcgatttt
      841 gaactcataa gggaagtaac aaaaaaactg caaaagtaca ataattatat atggccaaac
      901 taacctctat taaaaaacat cagtttttca cacttagttg agcttcatga aaaaatcatc
      961 gaggatttca ttgcagaata
//
LOCUS       DAZOZX010000400          977 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZOZX010000400 DAZOZX010000000
VERSION     DAZOZX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.408"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..506)
                     /gene="mxiE"
                     /locus_tag="ACHUPT_004535"
     CDS             complement(<1..506)
                     /gene="mxiE"
                     /locus_tag="ACHUPT_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR0821759.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSA"
     gene            complement(636..>977)
                     /gene="mxiM"
                     /locus_tag="ACHUPT_004536"
     CDS             complement(636..>977)
                     /gene="mxiM"
                     /locus_tag="ACHUPT_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR0821760.1"
                     /translation="SNSEKEWHIVPVSKDYFSIPNDLLWSFNTTNKSINVYSKCISGK
                     AVYSFNAGKFMGNFNVKEVDGCFMDAQKIAIDKLFSMLKDGVVLKGNKINDTILIEKD
                     GEVKLKLIRGI"
BASE COUNT          326 a          199 c          118 g          334 t
ORIGIN      
        1 gctgaagtga ttgtttgatt gtgcaaaaat acatctaata aaccatttac taaccgccaa
       61 gtgttcagtt gttctttgac ttttgcccct aaagcttttc tacataaaga tctaaaataa
      121 gcctccgaca caccataatg ctctgtcaga ctctttattc ttacctcctt ttctttctca
      181 atagttctca gaagataaag aaccaaatga tatgactcag tcattcgtat catagccagt
      241 gcattactta cattctggtc agatgaaaag atgaatctta acattaactt atcaagcatc
      301 gattcggtat tacaactatc ccgaagatca ttctcatgat agaaatgaat acatttttca
      361 gcatggctat gctctactag agcaccactg aaatagtccc aaaaagcaag aaattttgca
      421 caactattaa tgttaaagcg ataatgatag ttcacattat cactgaattt tatattttga
      481 tttcggacaa aaaaaaaccc ttccatgctg gagccagctc aattatgtta cgttctttat
      541 cttttacaag ctcaacgtta actggttcat gtccactaga gtaaatgtaa aacatattac
      601 ttgtatttag acctttatat ttactcacaa tacaattata tccctcgaat taattttaat
      661 ttaacttccc catccttctc tataaggatg gtatcattta tcttattacc ttttaaaaca
      721 accccgtctt tcagcataga aaatagttta tctatagcta tcttttgtgc atccatgaag
      781 cacccatcta cttccttaac attaaagttg cccatgaatt tacctgcatt aaaactataa
      841 accgccttac cactaataca tttagagtaa acatttatac ttttattggt tgtattaaac
      901 gaccataata aatcatttgg aatagaaaaa taatccttac ttacaggaac aatatgccat
      961 tctttttcag aattaga
//
LOCUS       DAZOZX010000401          971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZOZX010000401 DAZOZX010000000
VERSION     DAZOZX010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.409"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..78)
                     /locus_tag="ACHUPT_004537"
     CDS             complement(<1..78)
                     /locus_tag="ACHUPT_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0821761.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
     gene            482..>971
                     /gene="ospC2"
                     /locus_tag="ACHUPT_004538"
     CDS             482..>971
                     /gene="ospC2"
                     /locus_tag="ACHUPT_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR0821762.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIK"
BASE COUNT          335 a          179 c          155 g          302 t
ORIGIN      
        1 gcgctgatgt ccggcagtgc ttttgccgtt acgcaccacg ccttcagtag cggagcagga
       61 aggacatctg atggaaatgg aagctacgca agcaccttaa aatcaccatc atacactaaa
      121 tcagtaagtt ggcagcatta ccctcttttt gttgagcgcc catgattctt tcatttattg
      181 ttactaattt tttatgttat catcatgtta tgagcatgcc ctgttttgcg tctctatttt
      241 atctatcata ttctgagcat taactacata acaaggcgca agatatatat ccaaaaacaa
      301 ataatactgt tgcaataatt cctttcagta aaggaagtgt gattaaactc aggggatttc
      361 agagtgaaaa tcgtacaaca tattaaaata tgttcgttat aatacataca agaaaatatt
      421 aagttagata atattattct aaataactgc agataaaaac gcacataatg ggggatgttt
      481 tatgaaaata cctgaagcag taaatcatat taatgttcag aacaatattg atcttgttga
      541 tggaaaaata aatccgaaca aagatacaaa agcattacaa aaaaacatat catgcgtaac
      601 aaactcatcc tcttctggca taagtgaaaa acatttagat cactgtgctg atactgtgaa
      661 aagcttttta aggaagtcta tagcagcgca aagttatagt aaaatgttct ctcaagggac
      721 cagtttcaaa tcattaaatc tttctattga agcgccatct ggagcacggt catcatttcg
      781 gtcacttgag cacctcgata aggtgtccag acactatctt tctgaaataa tacaaaaaac
      841 tcatcctctt tcttcggatg aacgtcactt gctctctatt attattaatt ctgattttaa
      901 cttcagacat caaagtaacg ctaacctgtc aaataatact cttaatatta agtcctttga
      961 taaaattaag t
//
LOCUS       DAZOZX010000402          951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZOZX010000402 DAZOZX010000000
VERSION     DAZOZX010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.410"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            103..>951
                     /locus_tag="ACHUPT_004539"
     CDS             103..>951
                     /locus_tag="ACHUPT_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0821763.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          321 a          193 c          163 g          274 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct gacaacactt
      781 ccggaaagtg tcctgagatt agctcagaat gccttcgtaa atgttgcagg taatccattg
      841 tctggccata ccatgcgtac actacaacaa ataaccaccg gaccagatta ttctggtcct
      901 cgaatatttt tctctatggg aaattctgcc acaatttccg ctccagaaca c
//
LOCUS       DAZOZX010000403          934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZOZX010000403 DAZOZX010000000
VERSION     DAZOZX010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.411"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(56..244)
                     /locus_tag="ACHUPT_004540"
     CDS             complement(56..244)
                     /locus_tag="ACHUPT_004540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821764.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(541..779)
                     /locus_tag="ACHUPT_004541"
                     /pseudo
     CDS             complement(541..779)
                     /locus_tag="ACHUPT_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          246 a          181 c          217 g          290 t
ORIGIN      
        1 accgcgctga tggttcttat ccgttttatt gaatcggaga acaaactgtg agctatcagc
       61 ctggtggtct ggttttgcct gatttaagcc gccgtgggta tttgtccaag aatgcttcaa
      121 gctccaatcg ttttggcctg gtgaatggag atgggttaag caaagactct cgttgctggt
      181 gtactttttc gcgctggcgc tcaagcatgg ataatgcgat tgcctgtagt tcttcgttag
      241 tcattgtctg cacctggaaa gcaatttacc ggagtacctc acactacggc aaaaatgttt
      301 tattgttgca acttgcgtaa ttattcaggt cagtttggaa cgcactgtat aaacataaat
      361 aacctgatac tgttgtgtgt tttcaaagaa aacacaggta tttttcacat aaaccttccc
      421 cttttcatat tcgaccactt aatcaaaaaa cggttttata cttgttgccc ccactggggt
      481 taaatgtgag acatgtcgca ctttgtgcca tgtgtcctcg ctgattttag gcgcggttca
      541 ttcattcttt ctgttggcat aaagtatggt atcggttggg cgaatgaaag ggactcgggt
      601 catggtcaga caatacctga ggcgttaatt cgttgaaaga gattgtgaat gggggaataa
      661 ctgagtctta actgctgtgc gatagcccgg acagatagtt gctcgtcgaa tctgagacgt
      721 aatgattctg gcaaataagt gcgaaagccg gtgatatcta gtgttgtttg agagttcatc
      781 gttcgcgtcc attaatacag atggacgcga ttgttgccag acaggataat tttcacaaga
      841 catcgcagat agagtactta cgataatttt ttattttata caacagatgc ccacgaaaag
      901 aattattttc aatagacacc tgcattacac aaag
//
LOCUS       DAZOZX010000404          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZOZX010000404 DAZOZX010000000
VERSION     DAZOZX010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.412"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..200
                     /locus_tag="ACHUPT_004542"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHUPT_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACHUPT_004543"
     CDS             300..>923
                     /locus_tag="ACHUPT_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR0821765.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZOZX010000405          922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZOZX010000405 DAZOZX010000000
VERSION     DAZOZX010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.413"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(1..246)
                     /locus_tag="ACHUPT_004544"
                     /pseudo
     CDS             complement(1..246)
                     /locus_tag="ACHUPT_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            324..611
                     /locus_tag="ACHUPT_004545"
     CDS             324..611
                     /locus_tag="ACHUPT_004545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821766.1"
                     /translation="MNRTAFRAIHAVGVDTGRNCRKFPLRAAGNFRIPFVINTYKPED
                     QFFVSGIHKERNIQVCATLNGIVKIIKAQNLSFFVTGSFKPSGLFPPAPQK"
     gene            642..>922
                     /locus_tag="ACHUPT_004546"
     CDS             642..>922
                     /locus_tag="ACHUPT_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0821767.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYESASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT          221 a          277 c          203 g          221 t
ORIGIN      
        1 gtctccgtta tggcgtacac cacatcttcc agtaatcgcc gcttcaccgg actcaggacc
       61 ccgtatcacc atccggaacc ccttatccgc cacatgccag gagaaccttt ccataaactc
      121 gtcgccactc atcacaaggt tttcttctcg ttttgtccgg tggctgttgt aacgcaggcc
      181 aatttcatcc tgaccggcat aatgttccag gcggctcatc tgcaccggct gcttctccag
      241 ccagttaagc catcggtttc cctgctgaaa aatatcggca aaacggggaa gcatcagaaa
      301 ggcggaataa cgccatccgg ccagtgaacc gtaccgcatt cagggcaata catgccgtcg
      361 gcgttgatac cggcaggaat tgtcgcaaat tcccgctccg tgcagcgggc aatttccgga
      421 tacccttcgt catcaacacg tacaaaccag aagaccagtt ttttgtttcc ggcatccaca
      481 aagaacggaa tattcaggtc tgcgcaacac tcaacggcat cgtcaaaatt atcaaagcgc
      541 agaacctcag cttcttcgta accggctcat ttaaaccgtc tggtctgttt cctccggctc
      601 cacaaaaata atgtccatca ttttgaagtg gacactatcg tatggaatac cggacctgga
      661 tcactgaagc tttacgcctt catttcgaag aacatttacc acgggttgta gccggacgtc
      721 gcctgggcgt accaaaatca actgtctgtg gtatgttcgt gcgcttccgc aacgctggcc
      781 tttcatggcc tctgcccgca ggtatgtcgg agcaggaact tgatgccctt ctttacgaaa
      841 gtgcctccac agtacctgtc gtacttactg aaagcaccgt tatgcccaaa ctcccggtgg
      901 taaaaaaacg tccccggcgg cc
//
LOCUS       DAZOZX010000406          914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZOZX010000406 DAZOZX010000000
VERSION     DAZOZX010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.414"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(140..541)
                     /locus_tag="ACHUPT_004547"
                     /pseudo
     CDS             complement(140..541)
                     /locus_tag="ACHUPT_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          243 a          209 c          192 g          270 t
ORIGIN      
        1 acattatagt cggctaaatg aagtttactg taaaaaaacg atactttttt cgtaagcgat
       61 gagatgtgga gccagaaaaa tggctgcgtt acgtcattga actgtccttg acccacgata
      121 ttcagacagt cgttgtatct taagttgaca ttacccgctg acgtcgatat tcccttggtg
      181 agagataacc caacgcacta tgcggatggt tttcattgta atgctcgatc gccactgcaa
      241 ggttatgcaa tgccgttctt ggtttcggca tgaacgcgat gcagtcttcc ttcatcgttt
      301 tcatgaaccg ttctgctatt ccattactct gagggctact gactgccgtc gtgcagggct
      361 ccagattcaa ctctctggcg aactgccgtg tttcatgagc cgtataagcc gaaccgttat
      421 cggccactgg attggccgtt ccggtccttt atcgccatat cgctcctgac atgacatcct
      481 gcaccgttgc tttgtcataa tctcccgtgc ctgctgccca gtctatggct tcacgatcgc
      541 aatatggaac gtaacgctgt catcgggaga atcgtcaatg cggcattcgg ttaacgctta
      601 ccaatatcaa caccaaccgg atttgtggag cctccagtct gaaggggctt tatgcgttcc
      661 gtataggata ttatgatgct ggagttttgc gaagctccac tcctttccga acatttacac
      721 agatattgcc taagaaaagt gaaaccataa tatggttata gtccctttca gtgcaaatac
      781 ttagcttgtt gcacagagaa atagaagctg cataagctct ttcttcaaaa aatgtaaata
      841 aagttaaata taggaaaaat tacttaatct atcttaataa cggaaagatt ttgtatacaa
      901 tcactgttac agaa
//
LOCUS       DAZOZX010000407          905 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZOZX010000407 DAZOZX010000000
VERSION     DAZOZX010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.415"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(231..326)
                     /locus_tag="ACHUPT_004548"
                     /pseudo
     CDS             complement(231..326)
                     /locus_tag="ACHUPT_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(362..667)
                     /gene="ccdB"
                     /locus_tag="ACHUPT_004549"
     CDS             complement(362..667)
                     /gene="ccdB"
                     /locus_tag="ACHUPT_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR0821768.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(669..887)
                     /gene="ccdA"
                     /locus_tag="ACHUPT_004550"
     CDS             complement(669..887)
                     /gene="ccdA"
                     /locus_tag="ACHUPT_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR0821769.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          235 a          222 c          192 g          256 t
ORIGIN      
        1 tggctttgtt aaaagaaata agtaggagtt tttatgaagt tcattccatg aaatgaactt
       61 cagcactttt ttattataat aaggataaac aaaattggct gtaaaaaaac gatactgttt
      121 cagttaaccg tgaatggcct gtttgcaccg tattgttctt gtttttcggt tagttaaaca
      181 tcgtacttca tatttgaacg gtaagcggct cgtcagaacc gtattgatat ttactgagag
      241 gtcagatcaa ctttccaagg caacagatcg cgtacccggt ttgccggcca gtcctggata
      301 tgctcaatga cgtaacgcag ccattttgtc aacgccacga tttttgaccc tagcctgaca
      361 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
      421 gatcagccac ttcttccccg ataacgaaga tcggcacact ggccatatca gtggtcatca
      481 tgcgccagct ttcatccccg acatgcacca ccgggtaaag ctcacgggag actttatctg
      541 acagcaggcg ggcactggcc agggggatca ccatccgtcg tccgggcgtg tcaataatgt
      601 cactctgtac atccacaaac agacgataac gactctctct tttataggcg taaaccttaa
      661 actgcatttc accagtccct gttctcatcg gcaaaagagc cgttcatttc aataaaacgg
      721 gcgatctcag ccatcccctg ctgattttca gcctgccagc gttccgcacg cagacggcgg
      781 gcctcttttt gcatggccgt atttaccagg cctgagatat tcacgtttgc ggatttaagt
      841 aactgatagc tgtcactgtc tatagtgaca gtaatacgct gcttcatgat aagccctcaa
      901 aaaca
//
LOCUS       DAZOZX010000408          881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZOZX010000408 DAZOZX010000000
VERSION     DAZOZX010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.416"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..778
                     /locus_tag="ACHUPT_004551"
     CDS             <1..778
                     /locus_tag="ACHUPT_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821770.1"
                     /translation="DIIQVYGKILNMCNLSQETILTLLAAVGANNVSGLCMSFMNGHV
                     DTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFFALQNGHADSIRMFGSLLNKKM
                     LSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKILPPHQEYIDELLWIKNPNGTS
                     GLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSATNKDGIPGVFVSVVNRDKETI
                     LEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFNHFL"
BASE COUNT          315 a          134 c          167 g          265 t
ORIGIN      
        1 agacattatt caagtatatg gtaagatatt aaatatgtgc aatcttagtc aagaaacaat
       61 acttacattg cttgctgcgg taggggcaaa caatgtgtcg ggtttatgca tgtcttttat
      121 gaacggacat gtagacacaa taaaagccta tggggaaata gtctttaaaa ccccgttaac
      181 ttccgataag agattatatc tattagctgc gaaagacagc catgatttac ctgggttatt
      241 ttttgcattg cagaatggac atgcagatag tattcgtatg ttcggaagtc tgcttaataa
      301 gaaaatgctt agtagtgaac aaattaaaga attattgaaa gtaaagcatg ggttgtttat
      361 ggcacttcaa aatgggcaca caaaggccat tatggcgtat ggagatatac taaaaatact
      421 cccccctcac caggaatata tagatgaatt attatggata aaaaatccca acggtacatc
      481 aggacttttt atggcatttt ataatggcca taccgaaaca atacgtgcct tttgtaatat
      541 tctaaaaaac tatagtttta ccacaagacg attggtcgaa atgctgtccg caactaataa
      601 agatggtatt cctggtgtat ttgtatctgt agtaaataga gacaaagaaa caatattaga
      661 gtattgtaga ataataaaag agaacaatct ggaaccggat actatagcag aacaattttc
      721 aaagaagatg aaaaaaacgt ttattgaaat aataaataga tttaatcatt ttttatgaga
      781 ctgtaattta aattgtgtaa ttacctgttt ttgatatgtt cactccaaca aggtgtagtg
      841 accgggtaac tgccaacttt caggcaacca cctcaattgg c
//
LOCUS       DAZOZX010000409          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZOZX010000409 DAZOZX010000000
VERSION     DAZOZX010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.417"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHUPT_004552"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHUPT_004552"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0821771.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          238 a          215 c          189 g          235 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcagat
      781 ttaaagaaaa atacgaaaca tatcgttaat aattaaaggg agtaacgtat tatgtcagaa
      841 gaaaataaag aaaatggatt taatcatgtc aaaacat
//
LOCUS       DAZOZX010000410          873 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZOZX010000410 DAZOZX010000000
VERSION     DAZOZX010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.418"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            144..860
                     /locus_tag="ACHUPT_004553"
     CDS             144..860
                     /locus_tag="ACHUPT_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0821772.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          296 a          140 c          180 g          257 t
ORIGIN      
        1 ataaaattaa atataagatt atggctttag aatattttta tttctaatag acgagatttt
       61 tcctgttatg atataatatg ccgaattaac acatgttaac gatttaccag taatgtaaat
      121 aaattttcga ggagatcatt ccagtgggac gtaaatgggc caatattgtt gctaaaaaaa
      181 cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt cggtgtagaa atctatgctg
      241 ctgccaaaca aggtgaaccc gatccagaat taaacacatc tttaaaattc gttattgaac
      301 gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa agcaattgat aaagccaaag
      361 gcggcggaga tgaaacgttc gtacagggac gttatgaagg ctttggtcct aatggctcaa
      421 tgattattgc tgagacattg acttcaaatg ttaaccgtac gattgctaac gttcgcacaa
      481 ttttcaataa aaaaggcggc aatatcggag cggcaggttc tgtcagctat atgtttgaca
      541 atacgggtgt gattgtattt aaaggaacag accctgacca tatttttgaa attttgcttg
      601 aagctgaagt tgatgttcgt gatgtaactg aagaagaagg taatattgtt atttatactg
      661 aacctgctga ccttcataaa ggaatcgcgg cgctaaaagc agctggaatc actgagttct
      721 caacaacaga attagaaatg attgctcaat ctgaagttga gctttcccca gaagatttag
      781 aaatctttga agggcttgtt gatgcccttg aagatgacga cgatgtacaa aaagtttatc
      841 ataacgtcgc aaatctctaa ttatatttta aag
//
LOCUS       DAZOZX010000411          872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZOZX010000411 DAZOZX010000000
VERSION     DAZOZX010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.419"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(219..701)
                     /gene="sseA"
                     /locus_tag="ACHUPT_004554"
                     /pseudo
     CDS             complement(219..701)
                     /gene="sseA"
                     /locus_tag="ACHUPT_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          205 a          242 c          209 g          216 t
ORIGIN      
        1 taccgaacac ggcggaaagg gccggtgtcg ataatacgct aaataaacaa taattactct
       61 cttttgcttg acaaaaagag agttactggt gagtattgtt ttgctgtgaa gcacctgcgt
      121 tgctcatgta acacaagtca acctgctgga aaggccgcga accagaccag caataaaaaa
      181 acgccaaatt cggcggtttt ttattgcctg taatatactt atttcactgg ctcaaccggt
      241 aaatctgccc gcgcgcccca ttcactccat gcgccgtcgt acagtttcac gtttggcaca
      301 tccagcgtcg cgagtgctaa caaaaccacg gctgccgtta caccagagcc gcagctgacg
      361 ataatcggtt tgtcatagct gacgccgcga ccaaaaaata tcgcatccag ttcatcggtc
      421 gtttttagtt cgccttcgcg caccagttcc gtccacggaa cgttcaatgc accgggaata
      481 tgtccgcgac gtaaacctgg gcgaggttca tcaacttctg cgttaaaacg tgcagccggg
      541 cgggcatcaa taatttgcgc cgtattttca tggcttgcca ataacacatc ggttactttc
      601 accactgctt caggattaaa cgcggcgtta aactctcctt ccggcagctc tactgcacct
      661 tcttctaata gcaggtcatc gcgctgccag cctgcaagtc cccgttactt tattcgtacc
      721 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
      781 atccgggggg ggatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      841 tcaggcactt ttgccgggac cgtctgtaac gt
//
LOCUS       DAZOZX010000412          872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZOZX010000412 DAZOZX010000000
VERSION     DAZOZX010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.420"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..662)
                     /locus_tag="ACHUPT_004555"
     CDS             complement(<1..662)
                     /locus_tag="ACHUPT_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821773.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TG"
BASE COUNT          238 a          142 c          163 g          329 t
ORIGIN      
        1 tgccctgtca tgttgaaatc aataaagtcg catccagcaa gggcttccat cctattttca
       61 tttttactgt gctcaatata cagcttcgcc atttttaagt acaagctttg aatgtctctt
      121 atatcagttg gtaagtcagc tcgttgccca gcaaaatttt tattatcgga agaaacggtt
      181 tggacactga atctttcggg tattccttct tctacgagaa ttttagttat gattggattg
      241 ttgggttctg gattaacgac aacaatgatt tgacatacag aatccttatc cttttcgtat
      301 ctaatgttat gaatctgatt aagtattgat tgtgtctgtt gcgtaagtgg tgtcgaactt
      361 atcgtttcaa tgttattaat aagacttaca agttgaacca caacttttga gtagtctgca
      421 tttgtatatg tggtgttcgt gtggcatgca ttaatttgtt ggagttgttg ttgcgctaaa
      481 tcaaattgtt cagggttgta tccttctcca gagaatgtgg aattaatggg ttgttgttct
      541 tcccaattat cacaaggcca cttcggtgtg tctggcgttt taacattatc ggtatttgtt
      601 gtgcagttat aaatatcgcc gtgatatgta tttattatac ctggggtaaa gtcaattgac
      661 ataacattcc ttattcatca tctgttttat gctaatgttt ttaaattgga taattagaac
      721 aggaatggat tttcttctac acacaataaa tataatgcgt tatatcttcc tgatataatt
      781 acctttaaat tccctttcat ttccacccgg taatatttgt gtataaaaaa agggcagtga
      841 tagtatcgta aaggaaaacg ttaacaacta tc
//
LOCUS       DAZOZX010000413          870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZOZX010000413 DAZOZX010000000
VERSION     DAZOZX010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.421"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>870
                     /locus_tag="ACHUPT_004556"
     CDS             <1..>870
                     /locus_tag="ACHUPT_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0821774.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZOZX010000414          855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZOZX010000414 DAZOZX010000000
VERSION     DAZOZX010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.422"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..241
                     /gene="ipgA"
                     /locus_tag="ACHUPT_004557"
     CDS             <1..241
                     /gene="ipgA"
                     /locus_tag="ACHUPT_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR0821775.1"
                     /translation="NEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTK
                     KHYKKYLSPDELIESLYEFLFCIKLTIANITSEVN"
     gene            257..>855
                     /gene="ipgB1"
                     /locus_tag="ACHUPT_004558"
     CDS             257..>855
                     /gene="ipgB1"
                     /locus_tag="ACHUPT_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR0821776.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKA"
BASE COUNT          319 a          154 c          131 g          251 t
ORIGIN      
        1 taatgaacat gaacttttta cagaaaatat tattaatcca gaacacatac tcatccttaa
       61 ctactctttg agtagagatt atggttcttc tatctgtctt ctccctgata ctcatcaatg
      121 tgttttgacc aagaaacact acaaaaaata tctttcacct gatgaactca ttgaatcttt
      181 gtatgaattt ttattttgta ttaaactaac cattgcaaac atcacttcag aagtgaacta
      241 acatataggg ggtatcatgc aaattctaaa caaaatactt ccacaggttg aattcgcgat
      301 tcctcgccca tcatttgatt cgttaagtcg caataagctt gtaaagaaaa tattatctgt
      361 ttttaactta aaacaacgct ttcctcagaa gaattttgga tgcccggtga atataaataa
      421 aatcagggac agcgtcatag acaaaataaa agatagtaat tcggggaatc aactattttg
      481 ttggatgagc caagagcgaa ccacttatgt ctcttcaatg ataaacagaa gtatagatga
      541 aatggccata cacaatggag ttgttttgac ctcagataac aaaaaaaaca tatttgcagc
      601 tattgaaaaa aaattcccgg atataaaact tgatgaaaaa tcagcacaaa catctatcag
      661 tcatacagca ctaaacgaga ttgcctcatc tggcctcaga gcaaagattt taaaacgcta
      721 tagcagtgac atggatttgt ttaacacaca aatgaaggat cttacaaatc tagtatccag
      781 ttccgtctac gataaaatat ttaatgaatc aacaaaagtt ttacaaatag aaataagcgc
      841 agaggttcta aaggc
//
LOCUS       DAZOZX010000415          852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZOZX010000415 DAZOZX010000000
VERSION     DAZOZX010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.423"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>852)
                     /locus_tag="ACHUPT_004559"
     CDS             complement(<1..>852)
                     /locus_tag="ACHUPT_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR0821777.1"
                     /translation="ARTEATNDLYFSRVSQWDDWLSNYTTLQYRGQFDVVRPEVRKLV
                     AEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMRHNTAKIAVNVGVREQIESGVGAW
                     RLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDANSKQMDKSDAKHCTVINALSRNGW
                     KEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAEYYEVEEEKEKVFIYRDPLTG
                     EPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRVYKSIITCTQILKDREKIAGEHIP
                     IVPVYGEW"
BASE COUNT          179 a          237 c          207 g          229 t
ORIGIN      
        1 ccattcgcca tacactggaa cgattggaat atgctctccg gctatcttct cgcggtcttt
       61 cagtatctgc gtacaggtga tgatcgactt atacacacgc cgacgcttca ccttgcgctc
      121 tgctacctta atgaatccac gattagccag gtcgtcgatg acgtctttga tatcctgctg
      181 gtaatagctg accggctcac ctgtcagcgg gtcgcggtag atgaagacct tctccttctc
      241 ctcttctacc tcgtaatact cagcgacgta gacgacatca ttcgacaccc acgggaatag
      301 ccatgtatcg ttaggattct ggaaagatgg cagggtatcc gggtcaatac cgtaatcctc
      361 tgcgaactct ttccagccat tgcgcgacaa ggcgttaatc accgtgcagt gcttagcgtc
      421 gctcttatcc atctgcttgc tgttggcgtc ccatatgacg tgtgagcagg cttcatggat
      481 tggcaggcgt cggattacct gattgttgct tgttggatcg ttgtcttcgt actgcgtgac
      541 cagacgccat gcaccaacgc ctgactctat ctgctcacga acgccaacgt taacggcaat
      601 ctttgccgtg ttatggcgca tatcagtacg atacatcccc atcaatacat cggctgcatc
      661 aggattagcg ccgtctttgg gtcggaagag aacgtcgaca gggttccggc gcatctctgc
      721 gaccagtttc ctgacctccg ggcgaacaac atcgaattgt ccgcgatatt gcagggtagt
      781 gtagtttgat agccagtcat cccattgcga cactcggcta aaatacaggt catttgtcgc
      841 ctcggttctg gc
//
LOCUS       DAZOZX010000416          831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZOZX010000416 DAZOZX010000000
VERSION     DAZOZX010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.424"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHUPT_004560"
     CDS             <1..326
                     /locus_tag="ACHUPT_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0821778.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHUPT_004561"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHUPT_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR0821779.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAZOZX010000417          828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZOZX010000417 DAZOZX010000000
VERSION     DAZOZX010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.425"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            211..684
                     /locus_tag="ACHUPT_004562"
     CDS             211..684
                     /locus_tag="ACHUPT_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821780.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          257 a          172 c          183 g          216 t
ORIGIN      
        1 gagcaggtac cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct
       61 attagcggtt accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac
      121 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
      181 aagtaacggg ggggagatga accctatgca atgtcctggt taatatctca gtgtgctcat
      241 cagtgcactg ataataaaaa gacggagaca gatgccattt acgataaagt tcggagttct
      301 tatttgcttt catgtattct gaaaaaaaac aaaaatgttg ggcttattct tcatgcacca
      361 tcatttgttt ctgtgtctga aaaaatcgcc agaattgtga tggcaaatta ctcccgtaac
      421 tggagtaatt ccgagttggc cagtgcggtt ttaatgagtg aatcttcttt aaaaagaaga
      481 atgtacaaag aggtcggttc tataagtaca tttgtacata aaataaagct tacagaagct
      541 atcaggaaat tacgaaggac aaatactccc atttctgtca tatcatctga gttaggttac
      601 agttcaccat cgtatttttc taaagtcttc tttaaatatt taaagacata ccctcaaaac
      661 ataaggaaga aaaatggtcg gtaatgtcac caattaggag gacttgcaga gccgcttttt
      721 tacgatactg agatcaaaca gacataatct atgcggagat ttacagcagg gcatggttca
      781 caaatctgac agcgatgaat tatcggcact gcgggcagaa aatgctcg
//
LOCUS       DAZOZX010000418          827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZOZX010000418 DAZOZX010000000
VERSION     DAZOZX010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.426"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(282..357)
                     /locus_tag="ACHUPT_004563"
     tRNA            complement(282..357)
                     /locus_tag="ACHUPT_004563"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
BASE COUNT          254 a          184 c          135 g          254 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaatagg
//
LOCUS       DAZOZX010000419          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZOZX010000419 DAZOZX010000000
VERSION     DAZOZX010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.427"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            46..803
                     /locus_tag="ACHUPT_004564"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACHUPT_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZOZX010000420          799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZOZX010000420 DAZOZX010000000
VERSION     DAZOZX010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.428"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>799
                     /locus_tag="ACHUPT_004565"
     CDS             <1..>799
                     /locus_tag="ACHUPT_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0821781.1"
                     /translation="SKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISE
                     NIDKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFE
                     TKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEV
                     FMSLPVPVATMVREDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVN
                     ESILKKCCFENGSLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLK"
BASE COUNT          278 a          108 c          146 g          267 t
ORIGIN      
        1 tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact ttaacaaaga
       61 acaatatata tcctttataa aggacgcatt tattcatttt tatgattata gcaggcgata
      121 tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat tgatgagcac
      181 tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta cagaagaaga
      241 gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg ctgatgataa
      301 ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg cctttcaaga
      361 taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata tttgtagcgt
      421 aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga atgaagaagt
      481 tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa gtaatttagt
      541 caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt ttaatgacgt
      601 ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg tgaatgaaag
      661 catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa taacaaatag
      721 caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa ttaataacgt
      781 aaatactcat tctttaaaa
//
LOCUS       DAZOZX010000421          782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZOZX010000421 DAZOZX010000000
VERSION     DAZOZX010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.429"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..666)
                     /locus_tag="ACHUPT_004566"
     CDS             complement(<1..666)
                     /locus_tag="ACHUPT_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR0821782.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>782)
                     /locus_tag="ACHUPT_004567"
     CDS             complement(656..>782)
                     /locus_tag="ACHUPT_004567"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR0821783.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          151 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 tt
//
LOCUS       DAZOZX010000422          772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZOZX010000422 DAZOZX010000000
VERSION     DAZOZX010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.430"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..243
                     /locus_tag="ACHUPT_004568"
     CDS             <1..243
                     /locus_tag="ACHUPT_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0821784.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            358..>772
                     /locus_tag="ACHUPT_004569"
     CDS             358..>772
                     /locus_tag="ACHUPT_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0821785.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          206 a          198 c          201 g          167 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc
      301 ggattgtgcg atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg
      361 ccgatcatag caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa
      421 acacacgata aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac
      481 cgtgtcagcg acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt
      541 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
      601 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      661 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      721 ataaccggct gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tg
//
LOCUS       DAZOZX010000423          764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZOZX010000423 DAZOZX010000000
VERSION     DAZOZX010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.431"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>764
                     /locus_tag="ACHUPT_004570"
     CDS             <1..>764
                     /locus_tag="ACHUPT_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0821786.1"
                     /translation="KSAITIGDKNAYIDNLAGKNITNNGFDFKQTISTNLSIGETKFT
                     GGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTKGIDISGELT
                     MMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGNIYADDAATITLGQPE
                     TETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAIWHLNSQSSINRLETKD
                     SMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          255 a          156 c          154 g          199 t
ORIGIN      
        1 taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg cagggaaaaa
       61 tattactaat aatggttttg acttcaaaca aactatcagt actaatctat ccataggaga
      121 aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag gtgatcaagc
      181 tgtagttaca cttaatggtg caacctttct gaataatact cctataagta tagataaagg
      241 agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata tctccggtga
      301 actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg gtctccacta
      361 cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca gaaatatggc
      421 atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg gacagcctga
      481 aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc ttttgtatgg
      541 ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta gcatgaataa
      601 tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa aagacagtat
      661 ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca accttactat
      721 agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcag
//
LOCUS       DAZOZX010000424          759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZOZX010000424 DAZOZX010000000
VERSION     DAZOZX010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.432"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>759
                     /locus_tag="ACHUPT_004571"
     CDS             <1..>759
                     /locus_tag="ACHUPT_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0821787.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAZOZX010000425          720 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZOZX010000425 DAZOZX010000000
VERSION     DAZOZX010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.433"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..570)
                     /gene="fimE"
                     /locus_tag="ACHUPT_004572"
     CDS             complement(<1..570)
                     /gene="fimE"
                     /locus_tag="ACHUPT_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR0821788.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLIN"
BASE COUNT          222 a          178 c          144 g          176 t
ORIGIN      
        1 gtttatgaga ttatttcttt cccataatcc ggcaaaacga gcagcattac tggcggtata
       61 acgcacagta tggcgaatat ttcgatgccc gagataatcc tgaattaaac gggtatctgc
      121 accacgctcc gccagttcat aaccgcaagc atgccttaac atatgaggat gagtctgcgt
      181 tacggttcca gcttcaatac cggcatcgcg aataatgcga taggcctgct ggcgagaaag
      241 ccgactcccg cggcgagaaa taaatatagc gtcggtccgg tcagcgcctt tccagttagc
      301 acgttcctgg gtccagcgtt ccacggcttc acgctcatca aaacgtaacg ggtgaacggt
      361 agaaaatccg ttcttcagtc ggcgaatatt tattcgacct tcattaaggt caaggtcctg
      421 ataatgcaga tcaagcagtt cactaatacg catcccatgc cgatatgcca acagaataag
      481 acaataatct ctggctcccg ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc
      541 tttaccggta agataacgac gtttactcac aatagtagta ctcctaactg agatatattt
      601 gaatgaatac ctataggaaa cctcaatcgg tcaaaattag cccaatagat aaaaagataa
      661 ccacacacga agttatgtgg ctatcagtca attacattaa acatcataca aaaataaaat
//
LOCUS       DAZOZX010000426          714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZOZX010000426 DAZOZX010000000
VERSION     DAZOZX010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.434"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>714)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUPT_004573"
     CDS             complement(<1..>714)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUPT_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR0821789.1"
                     /translation="FYNTISGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNS
                     DELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVLP
                     PALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFLTELPA
                     FSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSSHALQALQRLTSSPD
                     YHGPRIYFSMSDGQQNTLHR"
BASE COUNT          196 a          123 c          180 g          215 t
ORIGIN      
        1 gcgatggagt gtattctgtt gtccgtcact catggagaag taaatccgtg ggccgtggta
       61 gtccggcgaa gaggttaatc tttgcagggc ttgcagagca tgggatgata atgggttatc
      121 actaatatgt attgaacatt cattcctcag attaagaatg ctttccggga tatgacttat
      181 ctgatttcta tcaaaaaaat actctctgac aacgggatta tttccctcag aaaatgctgg
      241 tagctcagtg aggaaattac gggtcgctct gagattttta agagcctgtg ggagcgatgg
      301 taaggagact atttcgttat agctgatatt catagtcaat aacgaatcgg gtaaagctgg
      361 caggttttcc agctcattgt gttgtacctg aagtgactcc agcgcaggag gtagcacggg
      421 caattctgat aatttattgc tggcggaata taattttttt agcgtaacag gcagttcagg
      481 taggttagtt aattgattat atgatacatt gagcagcgtt atctgagctg gtaagttgtc
      541 aggtagcgag gacagattta aacggtccag tcgaagttca tcggaattat tgataagaca
      601 ttctttaagt cgggagacag cctcatcacg ctcttcaccg gggagcgctt gtttttccca
      661 tttatcccat gctgaaaagt aatcagcata tgtaccggaa atagtgttat aaaa
//
LOCUS       DAZOZX010000427          711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZOZX010000427 DAZOZX010000000
VERSION     DAZOZX010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.435"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(48..178)
                     /locus_tag="ACHUPT_004574"
                     /pseudo
     CDS             complement(48..178)
                     /locus_tag="ACHUPT_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            176..259
                     /gene="tnpB"
                     /locus_tag="ACHUPT_004575"
                     /pseudo
     CDS             176..259
                     /gene="tnpB"
                     /locus_tag="ACHUPT_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          199 a          140 c          178 g          194 t
ORIGIN      
        1 tgaagtttac tgtaaaaaaa cgatactttt tcgggtggcg cttccaatca gtagaatatg
       61 tgtttttcaa tgaaggagcc gatgactttt cgtggatttc cacggaacgc gagaagcaga
      121 ttgttttacg tgccagccgc ttgatgcggg tgcggagggt caggttgtta cgttcaagat
      181 atagcatgtg ctggatgaag atcccttttc tggtcacctg tttatctttc gtggccgacg
      241 gggtgacact gttaaaatgt aatgaacgca tcccacacaa ttaaacacgg agtagtatga
      301 tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag ctttccggca tcatactgcc
      361 caatacggga tgcataaagc gtcctcagac tggagactcc acaaatgaaa tatacaccgg
      421 ttggcgttga tatggcaaaa catgtcattc agattcactt catcaatgag cacatgtcag
      481 cgccagtgat ataagacggt aatggggatg cggacgattt cctggactct cagaagatga
      541 aacatgttta ctgaagaaca aaaaattcgc gctattgagc tttatttcaa atatggtaag
      601 aagcttgagg tgtactggca atagcggaca ccaccatttg ttctttttct aagcagtcat
      661 ctgataatat tttatcctga aggctgccgg ggagatattc cccagacgag a
//
LOCUS       DAZOZX010000428          704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZOZX010000428 DAZOZX010000000
VERSION     DAZOZX010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.436"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..443)
                     /locus_tag="ACHUPT_004576"
     CDS             complement(<1..443)
                     /locus_tag="ACHUPT_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR0821790.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZOZX010000429          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZOZX010000429 DAZOZX010000000
VERSION     DAZOZX010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.437"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            32..493
                     /locus_tag="ACHUPT_004577"
     CDS             32..493
                     /locus_tag="ACHUPT_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0821791.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>696)
                     /locus_tag="ACHUPT_004578"
     CDS             complement(534..>696)
                     /locus_tag="ACHUPT_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0821792.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          160 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatg
//
LOCUS       DAZOZX010000430          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZOZX010000430 DAZOZX010000000
VERSION     DAZOZX010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.438"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..673
                     /locus_tag="ACHUPT_004579"
     rRNA            <1..673
                     /locus_tag="ACHUPT_004579"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAZOZX010000431          693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZOZX010000431 DAZOZX010000000
VERSION     DAZOZX010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.439"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            2..>693
                     /locus_tag="ACHUPT_004580"
     CDS             2..>693
                     /locus_tag="ACHUPT_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0821793.1"
                     /translation="MYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVAGTV
                     NAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAAS
                     GAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETV
                     GATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHD
                     LLSASLFEVTQW"
BASE COUNT          220 a          139 c          142 g          192 t
ORIGIN      
        1 aatatactcc cttaaatata gtgctgccac tggcggactc attgctgttt ctgaattagc
       61 gaaaagagtt tctggtaaaa caaaccgaaa acttgtagca acaatgttgt ctctggctgt
      121 tgcaggtaca gtaaatgcag caaatattga tatatcaaat gtatgggcga gagactatct
      181 tgatcttgca caaaataaag gtattttcca gcccggagca acagacgtaa caatcacttt
      241 aaaaaacgga gataaattct ctttccataa tctctcaatt ccggattttt ctggtgcagc
      301 agcgagtggc gcagctaccg caataggagg ttcttatagt gttactgttg cacataacaa
      361 aaagaaccct caggccgcag aaactcaggt ttacgctcag tcttcttaca aggttgttga
      421 cagaagaaat tccaatgatt ttgagattca gaggttaaat aaatttgttg tggaaacagt
      481 aggtgccacc ccggcagaga ccaaccctac aacatattct gatgcattag aacgctacgg
      541 tatagtcact tctgacggtt caaaaaaaat cataggtttt cgtgctggct ctggaggaac
      601 atcatttatt aatggtgaat ccaaaatctc aacaaattca gcatatagcc atgacctgtt
      661 aagtgctagt ctatttgagg tcacccaatg gga
//
LOCUS       DAZOZX010000432          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZOZX010000432 DAZOZX010000000
VERSION     DAZOZX010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.440"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(146..>690)
                     /locus_tag="ACHUPT_004581"
     CDS             complement(146..>690)
                     /locus_tag="ACHUPT_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0821794.1"
                     /translation="PLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGF
                     IVFSITAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIA
                     KIICGNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVY
                     VSDKIGHWLSRTVGGPFLIK"
BASE COUNT          239 a          118 c          113 g          220 t
ORIGIN      
        1 gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa aaccgtaaca
       61 tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat atctataaaa
      121 aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg actgtcctcg
      181 atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat atgattgcgc
      241 agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata gggataaata
      301 atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct cttacaataa
      361 agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag cctaccgtgg
      421 tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca ttgaatattg
      481 agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc attatagcgg
      541 taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca cgtgttaatt
      601 ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg gagtctatgt
      661 tccaaaacca ttgaggatct agtaatgggt
//
LOCUS       DAZOZX010000433          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZOZX010000433 DAZOZX010000000
VERSION     DAZOZX010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.441"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            52..685
                     /locus_tag="ACHUPT_004582"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHUPT_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZOZX010000434          679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZOZX010000434 DAZOZX010000000
VERSION     DAZOZX010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.442"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHUPT_004583"
     CDS             complement(<1..251)
                     /locus_tag="ACHUPT_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR0821795.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>679)
                     /locus_tag="ACHUPT_004584"
     CDS             complement(272..>679)
                     /locus_tag="ACHUPT_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0821796.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          152 g          216 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtacttcggt aatgactcca acttactgat agtgttttat
      661 gttcagataa tgcccgatg
//
LOCUS       DAZOZX010000435          678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZOZX010000435 DAZOZX010000000
VERSION     DAZOZX010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.443"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            2..678
                     /locus_tag="ACHUPT_004585"
                     /pseudo
     CDS             2..678
                     /locus_tag="ACHUPT_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          213 a          108 c          133 g          224 t
ORIGIN      
        1 tattaaaagc ctggctttcg actcttttaa agaaaggctt gagtctacca tggcggcact
       61 cactttcttt atatcaacgg gtcaatgtga ctgcgatgaa atagcagagt cgaacttcaa
      121 tcgtatgatt gcctatatgt ctaacattaa ctatgatgcc tcaaagctag gagcgccagc
      181 acttagtttt gatacgtatc tgcaggacaa tgttaaatat agagtgataa ttaataatct
      241 ttatggcagt gagtttcgga taagagatat taatgagaat tttgttggga tggattttac
      301 ctgtgaattc cgacccgaaa aaatgacgaa tttgatcaat ataaaaaata ggttggttat
      361 tcattattta aggactatat actatgaaaa aaattatata catcctgccg ggagcatttt
      421 tgctgcgatt caaaaaaaat gagagtctcc taaagtttcc ttcgataata tcaatgctta
      481 atatcaatct tctttttaat ccactaaatc ttccgggaat gggaagtggt gtgcttgaag
      541 atattatgag tattccagat agttcactta gaaaacgtct cgggtatgag gttttaagct
      601 tctcactaca ggcacattca ttgagccagg aatgtattga taagttggac atttttttcg
      661 ctgacgatct gtttaaat
//
LOCUS       DAZOZX010000436          672 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZOZX010000436 DAZOZX010000000
VERSION     DAZOZX010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.444"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..199
                     /locus_tag="ACHUPT_004586"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACHUPT_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          204 a          146 c          130 g          192 t
ORIGIN      
        1 cccacctgcc gggctaatcc accactccgc tcgaggttca cagtactgcg catacgatta
       61 ccgggtcata caggagcagt ctggtctgaa aacatcaatg tcgcgtaaag gtaactgtta
      121 cgacaacgct ccgatggaaa tcttctgggg aacgctgaaa aatgaaagcc tgagccacta
      181 tcgttttaag agccgggata tttcgtcagc atacgggaaa actgattgag gtcggcctca
      241 gttttgaggc ctttagccag ttcagccgca agggctttaa gcttcttttc gtccataatt
      301 tacctgtctc cgttgttgga gtaaacatat caaaaacagg caattacaca atttaaatta
      361 cagtctcaaa acaaacccaa cggtcaatat agtttaccag caagcttact agcaaagtgc
      421 taatcatttg gtatctttcg tgctattctc cttttacgta agtacttaag gggaacgtat
      481 ttactacttt taaagaaagc tccgttacta tctgaaatgc aatgagataa taaacgatta
      541 ctctcggtta gtatctcatg atcatttaca gactttgcat aatcgcgaat actatataac
      601 agagttttaa cattacagga tgaatagtct ctcttatcaa ctcctaataa aagaaggatt
      661 atggtcagtc gt
//
LOCUS       DAZOZX010000437          665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZOZX010000437 DAZOZX010000000
VERSION     DAZOZX010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.445"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(160..>665)
                     /locus_tag="ACHUPT_004587"
     CDS             complement(160..>665)
                     /locus_tag="ACHUPT_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0821797.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          175 a          165 c          146 g          179 t
ORIGIN      
        1 caccagcgcg tttaacgccc tgtgccattt ttcaggcaca aaaaaacccg cataaagcgg
       61 gttctttcaa gtgtccatgt ctgctattcg cctcgcgata cagctttgcg aagcttaccg
      121 taattgaagc agtttatgtg caaaaatgca agaacttttt taaagctgca tcaacctttc
      181 caccagttta tctctgcgaa caacaaacca accattggct ctcgccagtt ccagccatga
      241 ctcaagggaa ataacaatat catcatcccg taactgaatt gtggaaacag tgacaccgcc
      301 tctctgataa cagagaactc gcgtgtcgta acttttctgg catgaaactg gcgttgacgg
      361 atccctttga ctgaaatagc agtcttccag tttttcgaac acatcccacg cctgatcggt
      421 ttcgagcatt ttgccgtggc gtgctgcgcc tcgttctgtc cagaggatga gggagcgggc
      481 tttgggtgaa actggatttt gtgagtagtt taaagcgacc cgcaattctt taaggtcatt
      541 accaacaact ttgaaaaagt gtttcccttc aacgaagcgt actttgttct catgatgatt
      601 ctggcgaata cgcaccggct cagtgccgta aagctgcgcc aaaagttcgg tggtaataac
      661 aggaa
//
LOCUS       DAZOZX010000438          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZOZX010000438 DAZOZX010000000
VERSION     DAZOZX010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.446"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHUPT_004588"
     CDS             <1..>656
                     /locus_tag="ACHUPT_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821798.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZOZX010000439          654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZOZX010000439 DAZOZX010000000
VERSION     DAZOZX010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.447"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..534
                     /locus_tag="ACHUPT_004589"
                     /pseudo
     CDS             1..534
                     /locus_tag="ACHUPT_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          156 a          177 c          160 g          161 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attt
//
LOCUS       DAZOZX010000440          648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZOZX010000440 DAZOZX010000000
VERSION     DAZOZX010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.448"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            130..636
                     /gene="mug"
                     /locus_tag="ACHUPT_004590"
                     /pseudo
     CDS             130..636
                     /gene="mug"
                     /locus_tag="ACHUPT_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT          157 a          145 c          185 g          161 t
ORIGIN      
        1 gggccatggt agcggattat aaagtaactc cgtgccgcaa tccagccatt accgcgcgcc
       61 tgctgttttt ataaacaatg tattttcaat ccgttatact tttcctggtg atgttagaaa
      121 ggagtaaata tggttgagga tattttggct ccagggttac gggtcgtgtt ttgcggtatc
      181 aaccctgggc tttcatccgc cgggactggt tttccctttg ctcatccggc aaatcgcttc
      241 tggaaggtga tatatcaggc cgggtttacc gaccgtcagt tgaagccgca ggaggcacag
      301 catctgctgg attatcgttg tggcatcacc aaactggtag accgtccaac ggtgcaagcc
      361 aatgaagttt caaagcagga gctgcacgca ggtgggcgta agctgattga aaaaattgaa
      421 gattatcagc cgcaggcgtt ggctattctg ggcaaacaag catatgaaca gggattcagc
      481 tagcgcggtg cacagtgggg gaaacaaacg ctcaccattg gttcgacgca gatttgggtg
      541 ctgccaaatc ccagcggttt aagtcgcgtt tcactggaga aactggttga agcgtatcgc
      601 gagctggacc aggcgctggt agtgcgtggg cgataaaaaa cgtcaccg
//
LOCUS       DAZOZX010000441          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZOZX010000441 DAZOZX010000000
VERSION     DAZOZX010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.449"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUPT_004591"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUPT_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFR0821799.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHUPT_004592"
     CDS             complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHUPT_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0821800.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          146 c          155 g          174 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta ctgaggtgta ctggcaatag cggacactac catttgttct tttttaagca
      601 gccatctgat gatatttttc cctgaaggct gccggggaga t
//
LOCUS       DAZOZX010000442          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZOZX010000442 DAZOZX010000000
VERSION     DAZOZX010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.450"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..131
                     /gene="ospE1"
                     /locus_tag="ACHUPT_004593"
     CDS             <1..131
                     /gene="ospE1"
                     /locus_tag="ACHUPT_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFR0821801.1"
                     /translation="ELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            521..>640
                     /locus_tag="ACHUPT_004594"
     CDS             521..>640
                     /locus_tag="ACHUPT_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821802.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEF"
BASE COUNT          228 a          118 c          106 g          188 t
ORIGIN      
        1 acgaattaca aatacctgct gccagccgga cgaaaacgtt gaaattcact gctacagaaa
       61 tgcaatggtt gacaaagata aatgaagccg gaattgatga aaaacaaagc caacggtatt
      121 ctgatttctg aagttaattt cagactcgtt taagctccat tttcaaaaaa aatatatcag
      181 ggtttgttac taataacttc tgaaaacaga aaaaaataga gcagtaattc tatttcagag
      241 aggagtaact ccatctatcc tcgatacgta ctgaacgtta tcgtgatata ttcatcatgc
      301 tttcttcacc attattcgag tatagggaga gacgcaatga ccttaaatac ctttttatgg
      361 ctgaattacc attctcagga ataacagcag actccttccc atagacggct tagggggaaa
      421 gcaatacccg gagaaagggt acaaagcaaa ctgttttaaa attaataagc catattctat
      481 ttaactagaa ttcttatcgt tgataagaat tctagttata atgattaaat caaccaatat
      541 acaggcaatc ggttctggca ttatgcatca aataaacaat gtatactcgt taactccatt
      601 atctttacct atggaactga ctccatcttg taatgaattt
//
LOCUS       DAZOZX010000443          633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZOZX010000443 DAZOZX010000000
VERSION     DAZOZX010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.451"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(109..633)
                     /locus_tag="ACHUPT_004595"
                     /pseudo
     CDS             complement(109..633)
                     /locus_tag="ACHUPT_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          173 a          152 c          128 g          180 t
ORIGIN      
        1 gactgcttca gttttaaact cttcgggata acgcttaccg ctcatgggca cctctcttta
       61 agccatctta aatgactctg aggtgtctgt taaacccgtg gcgattcagg tctgcatagc
      121 attatcaaca ttcggatgtg caactacagc taatgcaaca tcatcccagc ttgcaatagt
      181 gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt taccactata
      241 atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag tccatgaacc
      301 agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc tgctgtttac
      361 gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag tcaacgtaat
      421 acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca gagtaatagc
      481 cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt aagtgccgtt
      541 atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca gcgccagacg
      601 gtcatggtag taatcatccg cacgatcagc aca
//
LOCUS       DAZOZX010000444          630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZOZX010000444 DAZOZX010000000
VERSION     DAZOZX010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.452"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>630
                     /locus_tag="ACHUPT_004596"
     CDS             <1..>630
                     /locus_tag="ACHUPT_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFR0821803.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQY"
BASE COUNT          147 a          160 c          178 g          145 t
ORIGIN      
        1 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
       61 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      121 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      181 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      241 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      301 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      361 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      421 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
      481 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
      541 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
      601 ggcaatggag cgcctgccgg cccagtatca
//
LOCUS       DAZOZX010000445          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZOZX010000445 DAZOZX010000000
VERSION     DAZOZX010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.453"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHUPT_004597"
     CDS             complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHUPT_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0821804.1"
                     /translation="RNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQ
                     NALISLPELPASLEYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINA
                     DNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHS
                     EETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          164 a          106 c          168 g          181 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgat
//
LOCUS       DAZOZX010000446          591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZOZX010000446 DAZOZX010000000
VERSION     DAZOZX010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.454"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>591
                     /locus_tag="ACHUPT_004598"
     CDS             <1..>591
                     /locus_tag="ACHUPT_004598"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR0821805.1"
                     /translation="STMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGGNH
                     PLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGI
                     AIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLP
                     DNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEV"
BASE COUNT          190 a           84 c          123 g          194 t
ORIGIN      
        1 ttcaacaatg cctgtgaaaa atttctccag tgtcggcgct ttttcatttc cgacctgcca
       61 tttttcaggg aatatacgta taggtcgatt ttgtagtatt gcatcaaatg taaaaataat
      121 gggtggaaat catcctttga atcgttttac tactcacatg atgacttata acggtgaatt
      181 tgataagttt gctatgagtg aatttgaacg tagctggaca ttgaaaccat tcattaccaa
      241 acctgaaaat ccgattatag gaaatgatgt ctggatagga aatgatgttg tattgaaagg
      301 tggcatcgca ataggtgatg gtgctgttat tgctgccaat tccgttgtta ccaaagatgt
      361 gcccccatat gccatagttg caggagttcc tgcaaaaata atcagattta ggtttgattc
      421 aaatgttatt gatgaactgt tgagaataaa atggtggaat tataactact cagatcttcc
      481 ggataataat aagtgtgatg atataaatta ttttgttgaa gagatgaatc gattgatatc
      541 aaatggaaat attcaagaga gggactataa aaaattcaat ctttctgagg t
//
LOCUS       DAZOZX010000447          580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZOZX010000447 DAZOZX010000000
VERSION     DAZOZX010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.455"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHUPT_004599"
     CDS             <1..318
                     /locus_tag="ACHUPT_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821806.1"
                     /translation="FSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGH
                     EMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNV
                     ASI"
     gene            321..>580
                     /locus_tag="ACHUPT_004600"
     CDS             321..>580
                     /locus_tag="ACHUPT_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0821807.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRR"
BASE COUNT          178 a          122 c          149 g          131 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg
//
LOCUS       DAZOZX010000448          570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZOZX010000448 DAZOZX010000000
VERSION     DAZOZX010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.456"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>570)
                     /locus_tag="ACHUPT_004601"
     CDS             complement(<1..>570)
                     /locus_tag="ACHUPT_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0821808.1"
                     /translation="SRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDV
                     IRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRA
                     KSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGG
                     LIIAAYQGEADGDKVIRLVRGQSYRGSRLG"
BASE COUNT          127 a          158 c          143 g          142 t
ORIGIN      
        1 tcccagtcgt gagcctctgt atgactgccc cctgacaagt ctgatgactt tatcaccgtc
       61 agcttctccc tggtacgcag caataatcag cccgccaatg tcagggtctc cccatctgtt
      121 gcggacagag ctcgccacga ttctgtaaat aatatcttct gtggttatat ttattttcac
      181 ccagccagtc agtctggata tgggccagta gcagtagcgg gtgtgataaa tggggccgtt
      241 aatgccgtaa aaagtaaggg atttggctct gtaccgcggt tctgttgtct cagggcgtgc
      301 atcagtccac cggatgctga gcaccccttc aaaccgtgtg tcgggtatga tgatgccgta
      361 ggggccagca acggaatatt cacctggcag cgcattcctt acccaggcca ggaaatcact
      421 cttagtgtca aaacggataa catcttcagg cagaaaagca cacccaaagc cgaatgcgcc
      481 gggtatcgcc agacggcctt ttgtccggtc gtaaatgtcg ctctgtgctt ccatcgtggc
      541 cgcacttttc agccccagat tatcccggga
//
LOCUS       DAZOZX010000449          568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZOZX010000449 DAZOZX010000000
VERSION     DAZOZX010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.457"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACHUPT_004602"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACHUPT_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0821809.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>568)
                     /locus_tag="ACHUPT_004603"
     CDS             complement(405..>568)
                     /locus_tag="ACHUPT_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0821810.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          105 g          145 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaatg
//
LOCUS       DAZOZX010000450          554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZOZX010000450 DAZOZX010000000
VERSION     DAZOZX010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.458"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>554)
                     /locus_tag="ACHUPT_004604"
     CDS             complement(<1..>554)
                     /locus_tag="ACHUPT_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR0821811.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          120 c          135 g          165 t
ORIGIN      
        1 cccatttaat atcaatctgt tgaaggtgta ttattacatc agtagcatat tccagactgc
       61 cttcctggac gatctgatcc ataacatcct cacgcacttt atatcttgtg catttcattt
      121 caagtgccgt gagtacagcc attactggtg atggtagctg tgttgttttc aggccgtcga
      181 tctgactgat cgcttgggcg acggtgtttt gccattctaa agaacaggtg ctgaagtcaa
      241 atgtggctgt tttattatcc cgttgcgaca cgctacaggc taagcgacgt aattgggtca
      301 atttgtctgc ttgctgaaca ggaactccgg gaaagcggcg atgacttaac gtggtgtgta
      361 caacttcggg cggaaaaacg acagcaatat ctttcgccag ccaaggtaaa acagcatctg
      421 tatttttgtc agccgcacgg gtttctgcta cgaaagaggg ttgttcctga ataatggtgg
      481 ataagtccgc taccttgaga gagtccgtta ccgtttggga aggttgcaaa gaattatctt
      541 tcgcttgctc caca
//
LOCUS       DAZOZX010000451          554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZOZX010000451 DAZOZX010000000
VERSION     DAZOZX010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.459"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>554
                     /locus_tag="ACHUPT_004605"
     CDS             <1..>554
                     /locus_tag="ACHUPT_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0821812.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEM"
BASE COUNT          125 a          134 c          162 g          133 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgtt
//
LOCUS       DAZOZX010000452          537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZOZX010000452 DAZOZX010000000
VERSION     DAZOZX010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.460"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..487
                     /locus_tag="ACHUPT_004606"
                     /pseudo
     CDS             1..487
                     /locus_tag="ACHUPT_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          124 a          118 c          136 g          159 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgtga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatc
//
LOCUS       DAZOZX010000453          532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZOZX010000453 DAZOZX010000000
VERSION     DAZOZX010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.461"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            381..>532
                     /locus_tag="ACHUPT_004607"
     CDS             381..>532
                     /locus_tag="ACHUPT_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0821813.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          131 a          140 c          115 g          146 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatagg tcttctctat ggtctctcta taggtctctc
       61 tagggtctcc tctaggcatt acccccagaa gctaactcta tggccctcta tggtcctcta
      121 tggccctgta cgtcattctt taggatgccc tgtagtttcg tatagcctga tgcctagaga
      181 gaccctagag agaccctaga gtggctccta gagagaccct cccagtagct ccgcacagct
      241 acatcgtcat catcatagta ttcaatctca aagtcattct gtttgcatcc tgttgtcctg
      301 ctggtctccc ttcgcttggg gactttgagt aggtcagtgg gggtgcgact tcagggtgca
      361 aacaaaaagg aaatcaagcg atgtctcaac aaccacaaca accacaacaa gaaatcagca
      421 tcattaacct cgaccagctt gtgtccatga cctcagttga aattgctgag ctgacaggca
      481 aggagcaccg aaatgtcctt cgtgatattc gtaagatgct cgaagaactt aa
//
LOCUS       DAZOZX010000454          531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZOZX010000454 DAZOZX010000000
VERSION     DAZOZX010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.462"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHUPT_004608"
     CDS             <1..228
                     /locus_tag="ACHUPT_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR0821814.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          183 a          111 c           84 g          153 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac t
//
LOCUS       DAZOZX010000455          529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZOZX010000455 DAZOZX010000000
VERSION     DAZOZX010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.463"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(21..173)
                     /locus_tag="ACHUPT_004609"
     CDS             complement(21..173)
                     /locus_tag="ACHUPT_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821815.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            191..382
                     /locus_tag="ACHUPT_004610"
     CDS             191..382
                     /locus_tag="ACHUPT_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0821816.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          141 a          124 c           91 g          173 t
ORIGIN      
        1 gaattattac gtttatcatg ttaattcatc attattacat catcattgta aataattaaa
       61 ttaacttcca taacattaaa atatgtatcc actgacgctt ttttacataa cgaagaattg
      121 accattttgt cctgttgtgc cttaatgtaa gtaccgtcca cagcgtggga catacttcaa
      181 ggaacctttt gtgagtcagg caactagtat gcgaaaacga caccgattta acagtcgcat
      241 gacccgtatc gtactgctca tcagctttat cttcttcttt ggccgtttta tctactcgtc
      301 cgtcggtgcc tggcagcacc atcagagcaa aaaagaagct cagcaatcca cactctccgt
      361 cgaatcaccg gtacaacgtt agcggttacc ttctccactt tcacagaaca taacggcact
      421 tcgctgtcgg atgcttttgc tctttgggat tatcaaagcg gcagatattc tttcatctta
      481 aattttacgt ctttgtcctg actgatgttt atcctgtttg gctgcgaaa
//
LOCUS       DAZOZX010000456          512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZOZX010000456 DAZOZX010000000
VERSION     DAZOZX010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.464"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            8..163
                     /locus_tag="ACHUPT_004611"
     CDS             8..163
                     /locus_tag="ACHUPT_004611"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821817.1"
                     /translation="MPVHLKAGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSR
                     YMEKTSC"
     gene            157..512
                     /locus_tag="ACHUPT_004612"
                     /pseudo
     CDS             157..512
                     /locus_tag="ACHUPT_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          147 a          119 c          123 g          123 t
ORIGIN      
        1 tccgctattg ccagtacacc tcaaggcagg cttaaagcct gacactatgc gtaacgtatt
       61 tcgctgccat ataccacgat acgaagcaat cattgctaga tatttggata tggaaccagc
      121 ccagatttgg ccttctcggt atatggagaa aacatcatgt taacccattg ggtaacagcg
      181 caagaatgcg taggaatgtc atgctttcct aaacatgagg tgagtattcg ccgcaatctt
      241 gaaaagcttg cagccaatca tccagaacta cgtcgcaagc gtacgggttc aaaggctttg
      301 gaatttcata tatcagttct accagcggca gcacgtgctg agttgttatt aaaatctgag
      361 caaatagaaa caagctaggg gtattttgaa atcgcccgcc ccacgctgga agcgcacgat
      421 tacgatcgtg aggcactgtg gagcaaatgg gataaagcca gcgattccca gcgcagactt
      481 gctgaaaaat ggttgcctgc ggttcagact gc
//
LOCUS       DAZOZX010000457          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZOZX010000457 DAZOZX010000000
VERSION     DAZOZX010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.465"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHUPT_004613"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHUPT_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZOZX010000458          504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZOZX010000458 DAZOZX010000000
VERSION     DAZOZX010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.466"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>504
                     /gene="mxiD"
                     /locus_tag="ACHUPT_004614"
     CDS             <1..>504
                     /gene="mxiD"
                     /locus_tag="ACHUPT_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR0821818.1"
                     /translation="QGTASFGDSFGASFNMSSSASISTLDGNKFIASVMALNQKKKAN
                     VVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFSSRGQIEM
                     SLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIGGYTHETNSNE
                     IISIPFLS"
BASE COUNT          163 a           80 c          107 g          154 t
ORIGIN      
        1 gcaaggaacg gcttcatttg gtgattcgtt tggtgcgtca tttaatatga gctcatcagc
       61 atcgataagc actttggacg gcaataagtt tattgcttca gttatggcat taaatcagaa
      121 aaagaaagca aatgtagttt caaggccggt gattttgaca caagagaata ttccggctat
      181 atttgataat aacaggactt tttatgtgtc attagttggt gagcgcaatt cttcgttgga
      241 gcatgttacg tatggaacat tgataaatgt tatacctcgc ttttcatctc ggggacaaat
      301 tgagatgtct ttaacgatag aggatggaac aggaaatagt cagtcaaatt acaattataa
      361 taatgaaaat acatctgtcc ttccagaagt tggaaggacc aaaatcagta caatagcgag
      421 agttccacaa ggcaaaagtt tgttgatagg tggttatacg catgaaacga actctaacga
      481 aataatatct attccttttc tctc
//
LOCUS       DAZOZX010000459          503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZOZX010000459 DAZOZX010000000
VERSION     DAZOZX010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.467"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..345)
                     /locus_tag="ACHUPT_004615"
     CDS             complement(<1..345)
                     /locus_tag="ACHUPT_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821819.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNILH"
BASE COUNT          161 a          107 c           76 g          159 t
ORIGIN      
        1 atggagaatg ttatcattgc tacaccctgt gcttaataac tgcaactctc cgccgttcag
       61 cgatgctttg ataattctgt tcccatagat atcgttatca aaataaagcg taaatatcat
      121 atttccgtaa ctgttatcat ctcttttttt gcacgtccga ataaacgcat tcagtaaatt
      181 actctctcta agaaaataaa tatcttcatt gttgttaatg acattaataa cgtcgttctt
      241 aaaatcctca aagtcttgtt gcggtaactt ctggttacgc tgcttactat cagcaagcgg
      301 tgaatccgga cgacaacaac ataggttttg tataaccccc agcattcttt tcacctccgt
      361 taacagtata ttcacgacac tgaaacgaga gaatgctgat aattttctcg gcgagatatc
      421 aaaaatcgct cattataaaa caagtaactt caacgtcgcg taaccgtgga cgataaaaaa
      481 acaataatgt cattcatatt taa
//
LOCUS       DAZOZX010000460          496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZOZX010000460 DAZOZX010000000
VERSION     DAZOZX010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.468"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>496)
                     /gene="rfbG"
                     /locus_tag="ACHUPT_004616"
     CDS             complement(<1..>496)
                     /gene="rfbG"
                     /locus_tag="ACHUPT_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR0821820.1"
                     /translation="DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQS
                     QTQFDYFFEAAGPGCTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYS
                     WYIDRKPTMLYRQHENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVN
                     NQLGK"
BASE COUNT          147 a           99 c           64 g          186 t
ORIGIN      
        1 ctttacccaa ttgattattc acaaagctat catcagctaa ggcattagct attttagtta
       61 cttcttttcg ataccacttg tttctcacca aaccaagccg tttgtatttt gctttaaaac
      121 taatgtttgc tcccacttgg ttgttttcat gctgtctgta cagcattgtt ggttttctat
      181 caatatacca cgaataattt ctcgtccgcg caaatgaata taaaaaccaa tcatgcagac
      241 agatatcttg tgcagcattt ttattattta tgatgaactt cttaaattca ataagcgttt
      301 ctttttttat aacatatgta caccccggac ctgctgcttc aaaaaaataa tcgaattgag
      361 tttgcgactg agccttatca actaaatttt ttcgccctga tggataatat gcaattacat
      421 tgctggaata acaattcgca ttattaaata ccaagcagtc aattgcattt tttattttat
      481 ctttatacca aatatc
//
LOCUS       DAZOZX010000461          491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZOZX010000461 DAZOZX010000000
VERSION     DAZOZX010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.469"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>491
                     /gene="ospB"
                     /locus_tag="ACHUPT_004617"
     CDS             <1..>491
                     /gene="ospB"
                     /locus_tag="ACHUPT_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR0821821.1"
                     /translation="NDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSH
                     QLGLGSELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILN
                     SLPFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPA
                     DPE"
BASE COUNT          154 a           88 c           91 g          158 t
ORIGIN      
        1 taatgatgat gacgcattga atgctgctgc ttatttttct gtaaaaaaag cgaaaataaa
       61 aacagtaaac gatactgatt ttaaagagta taataaggtt tatattcttg ggcacggtag
      121 tcctggttct catcaattag gccttggttc ggaacttatt gatgtacaaa caatcatttc
      181 aagaatgaaa gactgtggta ttctaaatgt gaaagatatc cgttttactt catgcggctc
      241 cgctgataaa gtggctccta aaaattttaa caatgcccct gctgaaagtc tttcttgtat
      301 ccttaactct ctgccttttt ttaaggaaaa agaatctttg ctagagcaga taaaaaaaca
      361 ccttgaaaac gatgagtcat tgagtgatgg tctaaaaata tccggctatc atggatatgg
      421 agttcactat ggtcaagagc tttttcccta ctcacattat cgttcaactt caattcctgc
      481 tgatccggag c
//
LOCUS       DAZOZX010000462          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZOZX010000462 DAZOZX010000000
VERSION     DAZOZX010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.470"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..347)
                     /gene="mxiC"
                     /locus_tag="ACHUPT_004618"
     CDS             complement(<1..347)
                     /gene="mxiC"
                     /locus_tag="ACHUPT_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR0821822.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYF"
     gene            complement(366..>489)
                     /locus_tag="ACHUPT_004619"
     CDS             complement(366..>489)
                     /locus_tag="ACHUPT_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821823.1"
                     /translation="NTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY"
BASE COUNT          142 a           96 c           68 g          183 t
ORIGIN      
        1 ttaaaatatc tctttagtct gtctataaaa tcccgatcta gaggaaggtt atcttttaac
       61 gttctcttta aatcaaaaat cttgtgatta atttcatctt cttccccatc taaaatacgt
      121 tcctgactat cactatttgt tcctttcagt ttctcaaggt ctcttctgtt tataaatgac
      181 gaaagagcgg aagccatctc atcagatgag tcaatatact tgctattagc caagccagaa
      241 tcaagctctg catcagcagt ctcatctcca tcatctgaat ttgtcattgc attcaactta
      301 tcaatgaatg cagagctaaa aactcctgta tttttaacat caagcataca tcacctactt
      361 tatagttagt aatttaagta tgaaaccaat gatttttcgt cttccagcaa agtagtttca
      421 gatgggatta tttgtgtagt cttttgtatt tctctttcag atatgaggct tttatattct
      481 gcagtatta
//
LOCUS       DAZOZX010000463          487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZOZX010000463 DAZOZX010000000
VERSION     DAZOZX010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.471"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>487
                     /locus_tag="ACHUPT_004620"
     CDS             <1..>487
                     /locus_tag="ACHUPT_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR0821824.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KR"
BASE COUNT           97 a          163 c          131 g           96 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgg
//
LOCUS       DAZOZX010000464          485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZOZX010000464 DAZOZX010000000
VERSION     DAZOZX010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.472"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..485
                     /locus_tag="ACHUPT_004621"
                     /pseudo
     CDS             1..485
                     /locus_tag="ACHUPT_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          178 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaaa
//
LOCUS       DAZOZX010000465          484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZOZX010000465 DAZOZX010000000
VERSION     DAZOZX010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.473"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..362
                     /locus_tag="ACHUPT_004622"
     CDS             <1..362
                     /locus_tag="ACHUPT_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0821825.1"
                     /translation="GLTEAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENM
                     YLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTS
                     SIKAKKNAITILNTLNL"
BASE COUNT          183 a          101 c           87 g          113 t
ORIGIN      
        1 acggattaac agaagcatgg ctcgaaagta gcttaaaaat ttcaccagaa gaacaaattc
       61 aattcctgcg taaaattatt aatcacaatc tcccagttaa aaactcagcc atagaaaaca
      121 ccatagagaa catgtatcta caagatctgg ataatagtac aaaactgtat gggaaaactg
      181 gtgcaggatt cacagcaaat agaaccttac aaaacggatg gtttgaaggg tttattataa
      241 gcaaatcagg acataaatat gtttttgtgt ccgcacttac aggaaacttg gggtcgaatt
      301 taacatcaag cataaaagcc aagaaaaatg cgatcaccat tctaaacaca ctaaatttat
      361 aaaaaatcta atggcaaaat cgcccaaccc ttcaatcaag tcgggacggc caaaagcaag
      421 cttttggctc ccctcgctgg cgctcggcgc cccttatttc aaacgttaaa catcatgagg
      481 gaag
//
LOCUS       DAZOZX010000466          483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZOZX010000466 DAZOZX010000000
VERSION     DAZOZX010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.474"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>483)
                     /locus_tag="ACHUPT_004623"
     CDS             complement(<1..>483)
                     /locus_tag="ACHUPT_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0821826.1"
                     /translation="EIAAVVAERDIEWWQNRASVLTTPQLASGYFNAGFLLINIDEWN
                     LNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKV
                     DNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYC
                     "
BASE COUNT          148 a          101 c           76 g          158 t
ORIGIN      
        1 cacaataccg atactgattg ctatttacag gtttaagtaa atcttcttta ctccacggag
       61 aagctgcttt ggcgatcaaa aaactacgtg aaaccggata atcagcccac tcatgccagg
      121 gttttgtagg tcccacatag tgtataaaca cggtgtcatc attgactgga ttatcaactt
      181 tgtcttttaa ttcatagtta atactatatc gggtattata tttctccgaa ataaacttca
      241 ctttaccatt caataataca ttcagtacat cttgatcaag gtgggtgatt ttacttaccc
      301 aatctgggtc acgcaacatt tcaatagctt ttgacgaaat gttatttaga ttccactcat
      361 caatatttat cagtaaaaaa ccagcattga aatatccaga agctaactgt ggtgtagtta
      421 ataccgaggc tcgattttgc caccattcaa tatctctttc agctacaact gcggcaattt
      481 cat
//
LOCUS       DAZOZX010000467          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZOZX010000467 DAZOZX010000000
VERSION     DAZOZX010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.475"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..473
                     /locus_tag="ACHUPT_004624"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACHUPT_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAZOZX010000468          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZOZX010000468 DAZOZX010000000
VERSION     DAZOZX010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.476"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            3..>472
                     /locus_tag="ACHUPT_004625"
     CDS             3..>472
                     /locus_tag="ACHUPT_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR0821827.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZOZX010000469          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZOZX010000469 DAZOZX010000000
VERSION     DAZOZX010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.477"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..361
                     /locus_tag="ACHUPT_004626"
     CDS             <1..361
                     /locus_tag="ACHUPT_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0821828.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAZOZX010000470          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZOZX010000470 DAZOZX010000000
VERSION     DAZOZX010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.478"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHUPT_004627"
     CDS             <1..>465
                     /locus_tag="ACHUPT_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0821829.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DAZOZX010000471          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZOZX010000471 DAZOZX010000000
VERSION     DAZOZX010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.479"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..461
                     /locus_tag="ACHUPT_004628"
                     /pseudo
     CDS             1..461
                     /locus_tag="ACHUPT_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          108 a          133 c          135 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc a
//
LOCUS       DAZOZX010000472          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZOZX010000472 DAZOZX010000000
VERSION     DAZOZX010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.480"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>459)
                     /locus_tag="ACHUPT_004629"
     CDS             complement(<1..>459)
                     /locus_tag="ACHUPT_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR0821830.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZOZX010000473          449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZOZX010000473 DAZOZX010000000
VERSION     DAZOZX010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.481"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..382)
                     /locus_tag="ACHUPT_004630"
     CDS             complement(<1..382)
                     /locus_tag="ACHUPT_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821831.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          112 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcggt
//
LOCUS       DAZOZX010000474          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZOZX010000474 DAZOZX010000000
VERSION     DAZOZX010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.482"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            213..>443
                     /locus_tag="ACHUPT_004631"
     CDS             213..>443
                     /locus_tag="ACHUPT_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR0821832.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZOZX010000475          437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZOZX010000475 DAZOZX010000000
VERSION     DAZOZX010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.483"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>437)
                     /locus_tag="ACHUPT_004632"
     CDS             complement(<1..>437)
                     /locus_tag="ACHUPT_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821833.1"
                     /translation="VILIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGG
                     NIFGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNK
                     ILWVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLQVAISIAS"
BASE COUNT          163 a           84 c           69 g          121 t
ORIGIN      
        1 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
       61 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
      121 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
      181 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
      241 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
      301 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
      361 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
      421 ttcgctatca atataac
//
LOCUS       DAZOZX010000476          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZOZX010000476 DAZOZX010000000
VERSION     DAZOZX010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.484"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            35..434
                     /locus_tag="ACHUPT_004633"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHUPT_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZOZX010000477          427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZOZX010000477 DAZOZX010000000
VERSION     DAZOZX010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.485"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..384
                     /gene="istB"
                     /locus_tag="ACHUPT_004634"
     CDS             <1..384
                     /gene="istB"
                     /locus_tag="ACHUPT_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR0821834.1"
                     /translation="VRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYL
                     PFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHV
                     VQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          122 a          103 c          107 g           95 t
ORIGIN      
        1 gttcgcttca caacagcagc agatctgtta cttcagttat ctacggcaca acgtcagggc
       61 cgttataaaa cgacgcttca gcgtggagta atggcccccc gcctgctcat cattgatgaa
      121 ataggctatc tgccgttcag tcaggaagaa gcaaaactgt tcttccaggt catcgctaaa
      181 cgttacgaaa agagcgcaat gatcctgaca tccaatctac cgttcgggca gtgggatcaa
      241 acgttcgccg gtgatgcagc actgacctca gcgatgctgg gccgtatctt acaccactca
      301 catgtcgttc aaatcaaagg agaaagctat cgactcagac agaaacgaaa ggccggggtt
      361 atagcagaag ctaatcctga gtaaaacggt ggatcaatat tgggccgttg gtggagatat
      421 aagtgga
//
LOCUS       DAZOZX010000478          409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZOZX010000478 DAZOZX010000000
VERSION     DAZOZX010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.486"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            155..340
                     /locus_tag="ACHUPT_004635"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACHUPT_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAZOZX010000479          407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZOZX010000479 DAZOZX010000000
VERSION     DAZOZX010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.487"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..269
                     /locus_tag="ACHUPT_004636"
     CDS             <1..269
                     /locus_tag="ACHUPT_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0821835.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>407
                     /gene="tnpB"
                     /locus_tag="ACHUPT_004637"
     CDS             266..>407
                     /gene="tnpB"
                     /locus_tag="ACHUPT_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0821836.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFR"
BASE COUNT           99 a          109 c          114 g           85 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggg
//
LOCUS       DAZOZX010000480          403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZOZX010000480 DAZOZX010000000
VERSION     DAZOZX010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.488"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>403)
                     /locus_tag="ACHUPT_004638"
     CDS             complement(<1..>403)
                     /locus_tag="ACHUPT_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821837.1"
                     /translation="DSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCS
                     ENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQV
                     DDAKFRRDNVKEIISTLFANKVDVDHPENKYK"
BASE COUNT          109 a           80 c           70 g          144 t
ORIGIN      
        1 tttgtattta ttttcaggat ggtcaacgtc aactttgtta gcgaataaag tagaaattat
       61 ttcttttaca ttatcgcgtc taaattttgc atcgtccact tgaaaattat atcttattgc
      121 tcgacctaaa cgtttaaaaa cttcgttagg ttctctcagt ctttccccgt tgagctcgtt
      181 tacaacctga tgatcggaac cagttgcaaa tgattctgcc atgatagctt tactgctttc
      241 aaaccacgct gaagcactcc tggcgttttc actacaatat tgggtgagac tatcaccatt
      301 aatacgaatt cctctttgaa tgtggtagtc gtcctgatcg tagtaaccga gagataagta
      361 atcatttccg tttttatttt taattacaat agcgagagag tcg
//
LOCUS       DAZOZX010000481          390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZOZX010000481 DAZOZX010000000
VERSION     DAZOZX010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.489"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(42..>390)
                     /locus_tag="ACHUPT_004639"
     CDS             complement(42..>390)
                     /locus_tag="ACHUPT_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0821838.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAZOZX010000482          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZOZX010000482 DAZOZX010000000
VERSION     DAZOZX010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.490"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHUPT_004640"
     CDS             <1..>375
                     /locus_tag="ACHUPT_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0821839.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZOZX010000483          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZOZX010000483 DAZOZX010000000
VERSION     DAZOZX010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.491"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHUPT_004641"
     CDS             <1..>374
                     /locus_tag="ACHUPT_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0821840.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAZOZX010000484          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZOZX010000484 DAZOZX010000000
VERSION     DAZOZX010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.492"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..>372)
                     /locus_tag="ACHUPT_004642"
     CDS             complement(<1..>372)
                     /locus_tag="ACHUPT_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0821841.1"
                     /translation="QESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDG"
BASE COUNT          116 a           70 c           60 g          126 t
ORIGIN      
        1 ccatcagagc ttctcgtggc cagaaggttc accagtttag attgagaaat ttcaccattt
       61 ttgtattttt ccatcagtat aaataatact tctgtcagat tctttgttat cgcatgatat
      121 ataactctgg aattgatcaa ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa
      181 ttatgcctat cttcaaatat tacctgcccg ggttgctcca gaccataaat tttccaataa
      241 aaatcatcaa aaaaatcctt agcggataat tgactcagtt cgaaatggct atcacaagta
      301 aatacagtac gtttatgagt aacagttcga ttaggatcga aaaactgaac aacccatttt
      361 tcactttctt gt
//
LOCUS       DAZOZX010000485          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZOZX010000485 DAZOZX010000000
VERSION     DAZOZX010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.493"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHUPT_004643"
     CDS             complement(<1..311)
                     /locus_tag="ACHUPT_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0821842.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZOZX010000486          369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZOZX010000486 DAZOZX010000000
VERSION     DAZOZX010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.494"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
BASE COUNT          115 a           66 c           79 g          109 t
ORIGIN      
        1 aaattggctg taaaaaaacg atactgtttc agttaactgt gaatagcttg cttgcaccgt
       61 attgttcttg tttttcggtt agttaaacat cgtacttcat atttgaacgt tctgccggaa
      121 tgcattatca atagaggtaa agtcgcaacc ccaaatcgta aaggaaaccg tagcacgtcg
      181 tatgcaagaa cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt tgaatgaaaa
      241 tcagccggag atgattttac ataattgcta cggaattatt caatacagga attgcttgcg
      301 tctgcatgga ttgacctgaa atattcccga aaatttctct aaaaaactcg aaaaaaatgg
      361 taactggtt
//
LOCUS       DAZOZX010000487          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZOZX010000487 DAZOZX010000000
VERSION     DAZOZX010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.495"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            37..336
                     /locus_tag="ACHUPT_004644"
                     /pseudo
     CDS             37..336
                     /locus_tag="ACHUPT_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013200692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          115 a           56 c           80 g          116 t
ORIGIN      
        1 ttaagatatg atgataagca gtttcataca acggttatca aagatgttct attatggatt
       61 gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc gggttatacc
      121 aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc tagctatatt
      181 cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa aactatcctt
      241 gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg atttaagtac
      301 atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt ggaggcaatg
      361 cactgag
//
LOCUS       DAZOZX010000488          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZOZX010000488 DAZOZX010000000
VERSION     DAZOZX010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.496"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHUPT_004645"
     CDS             <1..>365
                     /locus_tag="ACHUPT_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR0821843.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAZOZX010000489          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZOZX010000489 DAZOZX010000000
VERSION     DAZOZX010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.497"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
BASE COUNT          119 a           71 c           55 g          120 t
ORIGIN      
        1 tttaaataaa cagagatcca gattaaatac ctgagtataa aatctcttct gatttttaat
       61 tgatttgaat attcgtaagt tatatcactt actcaaccca ttttacccag agtcataaaa
      121 tagagtgtct gttgatctca ttttcatatt cctttcagac tgaagtgcat tttcatcacc
      181 cgatgtgaaa gaactgaagt gatgtttgcc atagattacg agatgcccag tctcattgac
      241 tatcgtggtg catctttata cctgttatac cagatcaaaa atcacgcaat ccatacaaca
      301 aaaccagatt tgcaattcgc gtcacaaaat atgtcgatct ttttctaaga agaagatgcc
      361 atgtg
//
LOCUS       DAZOZX010000490          362 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZOZX010000490 DAZOZX010000000
VERSION     DAZOZX010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.498"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            88..159
                     /gene="ysaE"
                     /locus_tag="ACHUPT_004646"
     CDS             88..159
                     /gene="ysaE"
                     /locus_tag="ACHUPT_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR0821844.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(136..288)
                     /gene="hokA"
                     /locus_tag="ACHUPT_004647"
     CDS             complement(136..288)
                     /gene="hokA"
                     /locus_tag="ACHUPT_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0821845.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
BASE COUNT          116 a           70 c           66 g          110 t
ORIGIN      
        1 ttttattttt tttcaggaaa taaataatcg atcgcgtaat aaaatctatt attatttttg
       61 tgaagaataa atatgggtgc aatgagaatg cgcaacgccg taagtaaggc gggaataatt
      121 tcccgtcgaa gactcttact ctttcaattt gtaggctaaa aacgccgcca gctcataact
      181 ctcctgttta atatgcaatt cacacagtga atctcttatc atccaggtga aaaataaaag
      241 cgtgaaacaa atcactatta aagaaagtaa tctatatttc tgcggcattc ccagccccct
      301 gtgttgattt ccaacgagta agaggctaac cttatggtgt tcaggcatag ggtagcctca
      361 ct
//
LOCUS       DAZOZX010000491          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZOZX010000491 DAZOZX010000000
VERSION     DAZOZX010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.499"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..264
                     /locus_tag="ACHUPT_004648"
     CDS             <1..264
                     /locus_tag="ACHUPT_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0821846.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="ACHUPT_004649"
     CDS             288..>353
                     /locus_tag="ACHUPT_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0821847.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAZOZX010000492          345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZOZX010000492 DAZOZX010000000
VERSION     DAZOZX010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.500"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            1..345
                     /locus_tag="ACHUPT_004650"
                     /pseudo
     CDS             1..345
                     /locus_tag="ACHUPT_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           74 a           90 c          106 g           75 t
ORIGIN      
        1 cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg ccgggcaacc
       61 gcccgcatta tgggcgttgg cctcaacacg attttccgcc atttaaaaaa ctcaggccgc
      121 agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg
      181 aacagtgggg atacgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca
      241 ggctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg acgctggggc
      301 gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatga
//
LOCUS       DAZOZX010000493          345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZOZX010000493 DAZOZX010000000
VERSION     DAZOZX010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.501"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="ACHUPT_004651"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="ACHUPT_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFR0821848.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAZOZX010000494          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZOZX010000494 DAZOZX010000000
VERSION     DAZOZX010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.502"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            2..172
                     /gene="xisR"
                     /locus_tag="ACHUPT_004652"
     CDS             2..172
                     /gene="xisR"
                     /locus_tag="ACHUPT_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR0821849.1"
                     /translation="MMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            174..>342
                     /locus_tag="ACHUPT_004653"
     CDS             174..>342
                     /locus_tag="ACHUPT_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR0821850.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           65 c           83 g           86 t
ORIGIN      
        1 cctgatgctt cgcactggtc tgggtgccag acaaattgaa gcataccgcc agaactgttg
       61 ggtggaaggc ttccacttca aacgagtatc tcctttaggg aagccagaca gtaagcgagg
      121 gattatctgg tacaactatc cgaagataaa tcagtttatc aaagactcat gatatgtcta
      181 aattaccaac aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag
      241 gaaaacgatg tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg
      301 ccgggaattt aagagcgttg atagttcatc aaatcagttc cg
//
LOCUS       DAZOZX010000495          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZOZX010000495 DAZOZX010000000
VERSION     DAZOZX010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.503"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
BASE COUNT          114 a           69 c           50 g          109 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagctcgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aa
//
LOCUS       DAZOZX010000496          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZOZX010000496 DAZOZX010000000
VERSION     DAZOZX010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.504"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>338
                     /locus_tag="ACHUPT_004654"
     CDS             <1..>338
                     /locus_tag="ACHUPT_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0821851.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLAD"
BASE COUNT           93 a           79 c           78 g           88 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcggatt
//
LOCUS       DAZOZX010000497          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZOZX010000497 DAZOZX010000000
VERSION     DAZOZX010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.505"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>335
                     /locus_tag="ACHUPT_004655"
     CDS             <1..>335
                     /locus_tag="ACHUPT_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0821852.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKER"
BASE COUNT          100 a           57 c          108 g           70 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agagg
//
LOCUS       DAZOZX010000498          333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZOZX010000498 DAZOZX010000000
VERSION     DAZOZX010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.506"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(43..330)
                     /locus_tag="ACHUPT_004656"
     CDS             complement(43..330)
                     /locus_tag="ACHUPT_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821853.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgt
//
LOCUS       DAZOZX010000499          333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZOZX010000499 DAZOZX010000000
VERSION     DAZOZX010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.507"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..206
                     /locus_tag="ACHUPT_004657"
     CDS             <1..206
                     /locus_tag="ACHUPT_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0821854.1"
                     /translation="VYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGL
                     KRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT           83 a           70 c          111 g           69 t
ORIGIN      
        1 tggtgtacat cataacggag acagtgagaa aaacggcgat gcaaatcacc tggagaggga
       61 tgtatcagag gttactgaag gttgacccgc tgaagtgcgt gctgtgcggg agtcagatgc
      121 gttttacggg gctgaagcgg ggctaccgtc tggcagagca ggtcctgatg catgagccgc
      181 tggcccggat gcggtggtgc ggctgagagc cgcagagggg aagttgcgtc cattttgagg
      241 gaaacggagc aaaaaaccgc cattcatacc ctgaatcaat cagagccatc cagtttaatc
      301 gtcggttccg ttcatatgga gcaaaagtgg tgt
//
LOCUS       DAZOZX010000500          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZOZX010000500 DAZOZX010000000
VERSION     DAZOZX010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.508"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            51..>332
                     /locus_tag="ACHUPT_004658"
     CDS             51..>332
                     /locus_tag="ACHUPT_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0821855.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAZOZX010000501          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZOZX010000501 DAZOZX010000000
VERSION     DAZOZX010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.509"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>332
                     /locus_tag="ACHUPT_004659"
     CDS             <1..>332
                     /locus_tag="ACHUPT_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0821856.1"
                     /translation="QYLLSLVPYCPWQHIVFTLPCQYWSLVFHNRWLLAEMSRIAADV
                     ILEICHQADVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMS
                     MWRYRITR"
BASE COUNT           71 a           88 c           95 g           78 t
ORIGIN      
        1 tacagtatct gctgagcctg gtcccctact gcccgtggca gcatattgtg ttcacacttc
       61 cctgccagta ctggtccctg gtgttccaca accggtggtt actggcagag atgagccgca
      121 ttgcagcgga tgtgatactg gaaatctgcc atcaggcaga tgtggagccg gggatattca
      181 cggtgatcca cacatggggg cgtgaccagc agtggcatcc gcatatccat ttatcgacaa
      241 ctgccggtgg tgtgacgtcg ggccacacct ggaaaaatct tcatttttac gcccgtaagg
      301 tgatgagcat gtggcgctac cggataacac gg
//
LOCUS       DAZOZX010000502          327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZOZX010000502 DAZOZX010000000
VERSION     DAZOZX010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.510"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>327
                     /locus_tag="ACHUPT_004660"
     CDS             <1..>327
                     /locus_tag="ACHUPT_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0821857.1"
                     /translation="SAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWVSE
                     MADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSL
                     PAAVWF"
BASE COUNT           82 a           76 c           99 g           70 t
ORIGIN      
        1 ccagtgcagg gttacttgca cggatcctgg tcagcaaata tatggaacat atccctttat
       61 atcgccagtc agaaatatac gcgcgacagg gcgtggagct gagccgtaat accatggtgc
      121 gctgggtatc agaaatggca gacaaactcc gtcctctgta tatagcgctg aatgactatg
      181 ttctggaggc aggaaaggtg cacgcagatg acactccggt gaaagtactg gccccgggga
      241 acggaaagac gaaaacgggt cgtctgtggg tatacgtcag ggatgatcgt aatgcgggtt
      301 catccctgcc ggcagccgtc tggttcg
//
LOCUS       DAZOZX010000503          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZOZX010000503 DAZOZX010000000
VERSION     DAZOZX010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.511"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            103..>325
                     /locus_tag="ACHUPT_004661"
     CDS             103..>325
                     /locus_tag="ACHUPT_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0821858.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAZOZX010000504          319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZOZX010000504 DAZOZX010000000
VERSION     DAZOZX010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.512"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            complement(<1..259)
                     /locus_tag="ACHUPT_004662"
     CDS             complement(<1..259)
                     /locus_tag="ACHUPT_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821859.1"
                     /translation="MNAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNS"
BASE COUNT           98 a           54 c           49 g          118 t
ORIGIN      
        1 cactattgta aaaccatatt ttctccatct gaagactatg ttcatttacc ctccagtgac
       61 catgttgcag gaaatagatg atatgcatgc caaaatcgtt atctatttgc gaatcattat
      121 ccagcttatc aatttcaact atctttgaac gatgtgtttt atcaaccttg ctatggtgtt
      181 gatgattatt aactttatct gaatgcttat ctttatggtt tgaagggatg tacttaaata
      241 tattgtcaat tgcgttcata aataacccgc gatatttttg acctgttagt aaaaaataaa
      301 tgatacatgt ctgaaagac
//
LOCUS       DAZOZX010000505          314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZOZX010000505 DAZOZX010000000
VERSION     DAZOZX010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.513"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
BASE COUNT           83 a           70 c           74 g           87 t
ORIGIN      
        1 ttaaaaaatg gagcaatatt aggcaaacga tttgagatat gacctaaaag ctcggccaaa
       61 taataatgtc gtcgattatc gaagtcatca tgagtgattc tgtccccaca gtgcagcatc
      121 agcatgacag tgaattgtct ggcgtggtcc atttaatgag atggagatgg tccctctttc
      181 ctaaatagct gtgcctaata ccgctacact tttgccagcc catgtttgcc tccggggaat
      241 gggctgacgg ttccttcaaa atccattgca cgcagttatg tcggagcggg aacttgactc
      301 ctgtctttac ggga
//
LOCUS       DAZOZX010000506          305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZOZX010000506 DAZOZX010000000
VERSION     DAZOZX010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.514"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
BASE COUNT           82 a           57 c           52 g          114 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tattt
//
LOCUS       DAZOZX010000507          293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZOZX010000507 DAZOZX010000000
VERSION     DAZOZX010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.515"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            132..>293
                     /locus_tag="ACHUPT_004663"
     CDS             132..>293
                     /locus_tag="ACHUPT_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0821860.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGIT"
BASE COUNT           90 a           57 c           56 g           90 t
ORIGIN      
        1 actgatatta ttcatcaaat aataacaaaa tagccattgc atcgggttgg acaatttaca
       61 aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata taaaatgatg
      121 gtcagattaa tatgcggatt atctttctac gcaaggagta tttatctcta ctcccgtcaa
      181 tgattgcatc tcttttctct gctaacggtg tcgcggcggc cattgattta tgccagggat
      241 atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt acg
//
LOCUS       DAZOZX010000508          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZOZX010000508 DAZOZX010000000
VERSION     DAZOZX010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.denovo.516"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..>289
                     /locus_tag="ACHUPT_004664"
     CDS             <1..>289
                     /locus_tag="ACHUPT_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0821861.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DAZOZX010000509         7240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.guided.168, whole genome shotgun sequence.
ACCESSION   DAZOZX010000509 DAZOZX010000000
VERSION     DAZOZX010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.guided.168"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..1062
                     /locus_tag="ACHUPT_004665"
     CDS             <1..1062
                     /locus_tag="ACHUPT_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR0821862.1"
                     /translation="GEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQ
                     AFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPV
                     RPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGE
                     QATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIY
                     RTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMA
                     RKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGG
                     EADAADKAEAANNKEKANG"
     gene            1055..1789
                     /gene="frdB"
                     /locus_tag="ACHUPT_004666"
     CDS             1055..1789
                     /gene="frdB"
                     /locus_tag="ACHUPT_004666"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0821863.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1800..2195
                     /gene="frdC"
                     /locus_tag="ACHUPT_004667"
     CDS             1800..2195
                     /gene="frdC"
                     /locus_tag="ACHUPT_004667"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0821864.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2206..2565
                     /gene="frdD"
                     /locus_tag="ACHUPT_004668"
     CDS             2206..2565
                     /gene="frdD"
                     /locus_tag="ACHUPT_004668"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0821865.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2628..3760
                     /locus_tag="ACHUPT_004669"
                     /pseudo
     CDS             2628..3760
                     /locus_tag="ACHUPT_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            3849..4382
                     /gene="blc"
                     /locus_tag="ACHUPT_004670"
     CDS             3849..4382
                     /gene="blc"
                     /locus_tag="ACHUPT_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0821866.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4379..4696)
                     /gene="sugE"
                     /locus_tag="ACHUPT_004671"
     CDS             complement(4379..4696)
                     /gene="sugE"
                     /locus_tag="ACHUPT_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0821867.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(4872..5018)
                     /gene="ecnB"
                     /locus_tag="ACHUPT_004672"
     CDS             complement(4872..5018)
                     /gene="ecnB"
                     /locus_tag="ACHUPT_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0821868.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(5129..5254)
                     /gene="ecnA"
                     /locus_tag="ACHUPT_004673"
     CDS             complement(5129..5254)
                     /gene="ecnA"
                     /locus_tag="ACHUPT_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0821869.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(5306..5872)
                     /gene="efp"
                     /locus_tag="ACHUPT_004674"
     CDS             complement(5306..5872)
                     /gene="efp"
                     /locus_tag="ACHUPT_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0821870.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            5914..6942
                     /gene="epmB"
                     /locus_tag="ACHUPT_004675"
     CDS             5914..6942
                     /gene="epmB"
                     /locus_tag="ACHUPT_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0821871.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1818 a         1858 c         1872 g         1692 t
ORIGIN      
        1 ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg
       61 ggcccggaaa ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac
      121 aaagtttctc aggccttctg gcacgaatgg cgtaaaggca acaccatctc cacgccgcgt
      181 ggtgatgtgg tttatctcga cctgcgtcac ctcggcgaga aaaaactgca tgaacgtctg
      241 ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt
      301 ccggtacgtc cgactgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa
      361 acccgcatta aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca
      421 aaccgtctgg gctccaactc cctggcggaa ctggtggtct tcggccgtct ggccggtgaa
      481 caagcgacag agcgtgcagc aactgccggt aatggcaacg aagcggcaat tgaagcgcag
      541 gcagctggcg ttgaacaacg tctgaaagat ctagttaacc aggatggcgg cgaaaactgg
      601 gcgaagatcc gcgacgaaat gggcctggca atggaagaag gttgcggtat ctaccgtacg
      661 ccggaactga tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc
      721 gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc tgctctacac cattgaactg
      781 agccacggtc tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc
      841 cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caactttctc
      901 aaacacaccc tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg
      961 aagattacta cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat
     1021 aaggcggaag cagccaataa taaggagaag gcgaatggct gagatgaaaa acctgaaaat
     1081 tgaggtggtg cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt
     1141 gccttatgac gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc
     1201 accggacctg agttaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat
     1261 ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat
     1321 gaaggttgaa gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca
     1381 cttcatcgaa agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga
     1441 tcagggtact aacatccaga ccccggcgca gatggcaaag tatcaccagt tctccggttg
     1501 catcaactgt ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat
     1561 cggtccggct gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa
     1621 gaaggagcgt atggcgcagt tgaacagcca gaacggtgta tggagctgta ctttcgtggg
     1681 ctattgctcc gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa
     1741 agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca
     1801 tgacgactaa acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc
     1861 cgttttatcg cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca
     1921 ttgaactgat tttcgcgctg tttgccctga aaaatggccc ggaagcctgg gcgggattcg
     1981 tcgacttttt acaaaacccg gttatcgtga tcattaacct gatcactctg acggcagctc
     2041 tgctgcacac caaaacctgg tttgaactgg caccgaaagc ggccaatatc attgtaaaag
     2101 acgaaaaaat gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca
     2161 ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat
     2221 ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc
     2281 gcaattattg cgccggtgat gatcctgctg gtgggtattc tgctgccact ggggctgttt
     2341 ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc
     2401 gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac
     2461 gcgatgcacg atctgaaaat ccacgtgcct gcgggcaaat gggttttcta cggtctggct
     2521 gctatcctga cagttgtcac gctgattggt gtcgttacaa tctaacgcat cgccaatgta
     2581 aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga
     2641 cgctctgcgc cttattaatt accgcctctt actccacatt tgctgcccct caacaaatca
     2701 acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg
     2761 cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg
     2821 ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat
     2881 ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatcccg
     2941 caacaaaata ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc
     3001 ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc ggatgaagtg aaatcctcaa
     3061 gcgacttgct gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc
     3121 tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga
     3181 gttttgagca ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga
     3241 ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg atatcgcgaa ggtaaggcag
     3301 tgcatgtttc gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag
     3361 atatggcccg ctgggtgcaa agcaatttac cccccttgat atcaatgaga aaacgcttca
     3421 acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct
     3481 gggctgggaa atgctggact ggccggtcaa tcctgacagc atcattaacg gcagtgacaa
     3541 taaaattgca ctggcagcac gcccggtaaa agcgattaag cccccaactc ctgcagtacg
     3601 cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg tcgcgtttat
     3661 tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca atccagcgag
     3721 agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt
     3781 ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg
     3841 aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg
     3901 cctgtagttt tcctacgccg ccgcgtggcg tgaccgtagt gaataatttc gacgccaaac
     3961 gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa cgtggactgg
     4021 aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc attaataaag
     4081 gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac tttaccggcg
     4141 caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg
     4201 ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac cgcgactacc
     4261 tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag
     4321 tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag cccggtagtt
     4381 agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga ctcgccagac
     4441 gcatcgggtt agcggactca ccgagcagca caatgccggt gatggccgcg ccgactgcgc
     4501 caatacccgt ccacacggca taagccgtcc ctaccggtaa cgatttcatc gcccaggcaa
     4561 gtagcgccat actgactatc atcgccgtca cggtaataac actcggcgtc aaacggctaa
     4621 agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta
     4681 agataatcca ggacatatca ggctccggaa atttggggcc gtccccggtg aaaagaggcg
     4741 tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa
     4801 tttcaactat tacctttatg acgatcgcag aaaaaacaaa cggcacgaca ctgctgtcgt
     4861 accgttattg cttattgctg cgctttcgtt gctgcgccag aaatcgcgtt accgccatca
     4921 gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc
     4981 agaacagaaa agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg
     5041 ttatttatgt ttgcctatag caaattaata atagacaaca tcgccaaaaa tgatggaaat
     5101 ctgatgattt ttcggaagag aagaaaaatt agctggcagc gcgagagatg gagttaccga
     5161 gatgtttgat gtcttcgccg aaaccgcaag cggtgttaca gcccgtgagc agcgtgctgg
     5221 caagcaaaac aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca
     5281 gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg
     5341 tatccacttt gatgacttcg ccgatttgta cgaacagcgg aactttaacc acagcgccag
     5401 tagacagggt agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg
     5461 tatcaacgat ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc
     5521 acagagttac gatacactct gcctgatcca gcagccattt agcattgtcg ccaattgctt
     5581 ttgcatcagc agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt
     5641 cgttgtacag gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag
     5701 atttgaaggt tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa
     5761 atgcctggcc tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt
     5821 ctaacatgat tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct
     5881 ctgaaatttg ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc
     5941 ccatccagag aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt
     6001 ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag
     6061 ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac
     6121 gatcctcttt tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gtccggattc
     6181 tccaccgacc cactggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac
     6241 aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt
     6301 cgccacttcc cctatgccga aaatcagggc aacaagcgta actggcagac tgcacttgag
     6361 tatgttgctg cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg
     6421 gcgaaagagc acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa
     6481 cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg
     6541 gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc
     6601 aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc
     6661 ctgcttaacc agagcgttct gttacgtggt gtgaacgata acgcacaaac gttggcaaac
     6721 ctgagtaatg cgttgttcga tgccggcgtg atgccgtatt acctgcatgt gctagataaa
     6781 gtacagggcg cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag
     6841 ttgctgacat tggtgtcggg atatctggtg ccgaaactgg cgcgagaaat tggcggcgaa
     6901 cccagcaaaa cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt
     6961 taaacattaa aattgcctga tgcgctacgc ttatcaggcc tacatcgtgc attgaaattg
     7021 aatggatttg caatgaactg taggccggat aaggcgttta cgccgcatcc ggcaagagca
     7081 acaaacgcaa gtacagcaac cactgagaaa tttaatcttt gcacgttgca taacctttaa
     7141 ttattcatat gcattgctgt tgttactttt tatatagcta acaccaataa ttaacctttc
     7201 ttttcggtaa tgactccaac ttactgatag tgttttatgt
//
LOCUS       DAZOZX010000510         7053 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.guided.172, whole genome shotgun sequence.
ACCESSION   DAZOZX010000510 DAZOZX010000000
VERSION     DAZOZX010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.guided.172"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            59..1102
                     /locus_tag="ACHUPT_004676"
     CDS             59..1102
                     /locus_tag="ACHUPT_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0821872.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUPT_004677"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUPT_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUPT_004678"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUPT_004678"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0821873.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUPT_004679"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUPT_004679"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0821874.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUPT_004680"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUPT_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0821875.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="ACHUPT_004681"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="ACHUPT_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0821876.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHUPT_004682"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHUPT_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0821877.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1716 a         1735 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DAZOZX010000511         7021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.guided.174, whole genome shotgun sequence.
ACCESSION   DAZOZX010000511 DAZOZX010000000
VERSION     DAZOZX010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.guided.174"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHUPT_004683"
     CDS             263..922
                     /locus_tag="ACHUPT_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR0821878.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHUPT_004684"
     CDS             complement(1123..1500)
                     /locus_tag="ACHUPT_004684"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0821879.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="ACHUPT_004685"
     CDS             complement(1567..4533)
                     /locus_tag="ACHUPT_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR0821880.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="ACHUPT_004686"
     CDS             complement(4536..5096)
                     /locus_tag="ACHUPT_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0821881.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="ACHUPT_004687"
                     /pseudo
     CDS             complement(5222..5572)
                     /locus_tag="ACHUPT_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHUPT_004688"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHUPT_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0821882.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
BASE COUNT         1449 a         1966 c         2084 g         1522 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 c
//
LOCUS       DAZOZX010000512         4295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.guided.221, whole genome shotgun sequence.
ACCESSION   DAZOZX010000512 DAZOZX010000000
VERSION     DAZOZX010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.guided.221"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHUPT_004689"
     CDS             558..797
                     /locus_tag="ACHUPT_004689"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0821883.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUPT_004690"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUPT_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUPT_004691"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUPT_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0821884.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUPT_004692"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUPT_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0821885.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHUPT_004693"
                     /pseudo
     CDS             3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHUPT_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAZOZX010000513         4292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.guided.222, whole genome shotgun sequence.
ACCESSION   DAZOZX010000513 DAZOZX010000000
VERSION     DAZOZX010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.guided.222"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHUPT_004694"
     CDS             558..797
                     /locus_tag="ACHUPT_004694"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0821886.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUPT_004695"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUPT_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUPT_004696"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUPT_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0821887.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUPT_004697"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUPT_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0821888.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHUPT_004698"
                     /pseudo
     CDS             3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHUPT_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAZOZX010000514         2588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.guided.289, whole genome shotgun sequence.
ACCESSION   DAZOZX010000514 DAZOZX010000000
VERSION     DAZOZX010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.guided.289"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            41..430
                     /locus_tag="ACHUPT_004699"
                     /pseudo
     CDS             41..430
                     /locus_tag="ACHUPT_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHUPT_004700"
     CDS             complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHUPT_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR0821889.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHUPT_004701"
     CDS             1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHUPT_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR0821890.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2431..>2588)
                     /locus_tag="ACHUPT_004702"
     CDS             complement(2431..>2588)
                     /locus_tag="ACHUPT_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR0821891.1"
                     /translation="LKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGF
                     LLSLKDK"
BASE COUNT          671 a          503 c          549 g          865 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc aaccatggcg tacatgccaa
       61 agtttccacg ccagtgcatt tacgaaaagc acgtacttgc tatgatcatt tagctggcga
      121 agttgccgtt aagatctatg attccctttg tcaacagcaa tggatcactg aaaatggttc
      181 aatgatcaca ttaagtggta ttcaatattt tcatgaaatg ggaattgacg ttccttccaa
      241 acattcacgt aaaatctgtt gtgcgtgttt agattggagt gaacgccgtt tccatttagg
      301 tgggtacgtt ggagccgcat tattttcgct ttatgaatct aaagggtggt taactcgaca
      361 tcttggttac cgtgaagtta ccatcacgga aaaaggttat gctgctttta agacccactt
      421 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
      481 ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
      541 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg
      601 caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
      661 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
      721 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      781 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      841 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      901 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      961 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
     1021 aatctagaca tcattaattc ctaatttttg ttgacactct atcattgata gagttatttt
     1081 accactccct atcagtgata gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg
     1141 taattacgtt actcgatgcc atggggattg gccttatcat gccagtcttg ccaacgttat
     1201 tacgtgaatt tattgcttcg gaagatatcg ctaaccactt tggcgtattg cttgcacttt
     1261 atgcgttaat gcaggttatc tttgctcctt ggcttggaaa aatgtctgac cgatttggtc
     1321 ggcgcccagt gctgttgttg tcattaatag gcgcatcgct ggattactta ttgctggctt
     1381 tttcaagtgc gctttggatg ctgtatttag gccgtttgct ttcagggatc acaggagcta
     1441 ctggggctgt cgcggcatcg gtcattgccg ataccacctc agcttctcaa cgcgtgaagt
     1501 ggttcggttg gttaggggca agttttgggc ttggtttaat agcggggcct attattggtg
     1561 gttttgcagg agagatttca ccgcatagtc ccttttttat cgctgcgttg ctaaatattg
     1621 tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa aaatacacgt gataatacag
     1681 ataccgaagt aggggttgag acgcaatcga attcggtata catcacttta tttaaaacga
     1741 tgcccatttt gttgattatt tatttttcag cgcaattgat aggccaaatt cccgcaacgg
     1801 tgtgggtgct atttaccgaa aatcgttttg gatggaatag catgatggtt ggcttttcat
     1861 tagcgggtct tggtctttta cactcagtat tccaagcctt tgtggcagga agaatagcca
     1921 ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat tgcagatagt agtgcatttg
     1981 cctttttagc gtttatatct gaaggttggt tagttttccc tgttttaatt ttattggctg
     2041 gtggtgggat cgctttacct gcattacagg gagtgatgtc tatccaaaca aagagtcatc
     2101 agcaaggtgc tttacaggga ttattggtga gccttaccaa tgcaaccggt gttattggcc
     2161 cattactgtt tgctgttatt tataatcatt cactaccaat ttgggatggc tggatttgga
     2221 ttattggttt agcgttttac tgtattatta tcctgctatc gatgaccttc atgttaaccc
     2281 ctcaagctca ggggagtaaa caggagacaa gtgcttagtt atttcgtcac caaatgatgt
     2341 tattccgcga aatataatga ccctcttgat aacccaagag ggcatttttt acgataaaga
     2401 agatttagct tcaaataaaa cctatctatt ttatttatct ttcaagctca ataaaaagcc
     2461 gcggtaaata gcaataaatt ggcctttttt atcggcaagc tcttttaggt ttttcgcatg
     2521 tattgcgata tgcataaacc agccattgag taagttttta agcacatcat catcataagc
     2581 tttaagtt
//
LOCUS       DAZOZX010000515         1357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.guided.371, whole genome shotgun sequence.
ACCESSION   DAZOZX010000515 DAZOZX010000000
VERSION     DAZOZX010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.guided.371"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..393
                     /locus_tag="ACHUPT_004703"
     CDS             <1..393
                     /locus_tag="ACHUPT_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0821892.1"
                     /translation="QDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPVK
                     NSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVSA
                     LTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            506..1344
                     /gene="aadA1"
                     /locus_tag="ACHUPT_004704"
                     /pseudo
     CDS             506..1344
                     /gene="aadA1"
                     /locus_tag="ACHUPT_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          402 a          317 c          328 g          310 t
ORIGIN      
        1 caagacttct ctggagataa agaaagaaac aacggattaa cagaagcatg gctcgaaagt
       61 agcttaaaaa tttcaccaga agaacaaatt caattcctgc gtaaaattat taatcacaat
      121 ctcccagtta aaaactcagc catagaaaac accatagaga acatgtatct acaagatctg
      181 gataatagta caaaactgta tgggaaaact ggtgcaggat tcacagcaaa tagaacctta
      241 caaaacggat ggtttgaagg gtttattata agcaaatcag gacataaata tgtttttgtg
      301 tccgcactta caggaaactt ggggtcgaat ttaacatcaa gcataaaagc caagaaaaat
      361 gcgatcacca ttctaaacac actaaattta taaaaaatct aatggcaaaa tcgcccaacc
      421 cttcaatcaa gtcgggacgg ccaaaagcaa gcttttggct cccctcgctg gcgctcggcg
      481 ccccttattt caaacgttaa acatcatgag ggaagtggtg atcgccgaag tatcgactca
      541 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      601 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      661 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      721 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      781 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      841 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      901 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      961 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     1021 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1081 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1141 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1201 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgttcacta
     1261 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg
     1321 acgccgcttc gcggcgcggc ttaactcaag cgttaga
//
LOCUS       DAZOZX010000516         1354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2007734
            SAMN43277559-rid23486353.guided.372, whole genome shotgun sequence.
ACCESSION   DAZOZX010000516 DAZOZX010000000
VERSION     DAZOZX010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277559
            Sequence Read Archive: SRR30464346
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396697.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396697.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,134
            CDSs (with protein)               :: 4,134
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 483
            CDSs (without protein)            :: 483
            Pseudo Genes (ambiguous residues) :: 0 of 483
            Pseudo Genes (frameshifted)       :: 229 of 483
            Pseudo Genes (incomplete)         :: 158 of 483
            Pseudo Genes (internal stop)      :: 168 of 483
            Pseudo Genes (multiple problems)  :: 68 of 483
            Pseudo Genes (short protein)      :: 1 of 483
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277559-rid23486353.guided.372"
                     /strain="M2007734"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-28"
                     /collected_by="FSS"
     gene            <1..393
                     /locus_tag="ACHUPT_004705"
     CDS             <1..393
                     /locus_tag="ACHUPT_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0821893.1"
                     /translation="QDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPVK
                     NSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVSA
                     LTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            506..1341
                     /gene="aadA1"
                     /locus_tag="ACHUPT_004706"
                     /pseudo
     CDS             506..1341
                     /gene="aadA1"
                     /locus_tag="ACHUPT_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          399 a          317 c          328 g          310 t
ORIGIN      
        1 caagacttct ctggagataa agaaagaaac aacggattaa cagaagcatg gctcgaaagt
       61 agcttaaaaa tttcaccaga agaacaaatt caattcctgc gtaaaattat taatcacaat
      121 ctcccagtta aaaactcagc catagaaaac accatagaga acatgtatct acaagatctg
      181 gataatagta caaaactgta tgggaaaact ggtgcaggat tcacagcaaa tagaacctta
      241 caaaacggat ggtttgaagg gtttattata agcaaatcag gacataaata tgtttttgtg
      301 tccgcactta caggaaactt ggggtcgaat ttaacatcaa gcataaaagc caagaaaaat
      361 gcgatcacca ttctaaacac actaaattta taaaaaatct aatggcaaaa tcgcccaacc
      421 cttcaatcaa gtcgggacgg ccaaaagcaa gcttttggct cccctcgctg gcgctcggcg
      481 ccccttattt caaacgttaa acatcatgag ggaagtggtg atcgccgaag tatcgactca
      541 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      601 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      661 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      721 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      781 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      841 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      901 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      961 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     1021 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1081 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1141 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctaggc aggcttatct
     1201 tggacaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg ttcactacgt
     1261 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt caagccgacg
     1321 ccgcttcgcg gcgcggctta actcaagcgt taga
//
